LOCUS       DASTYP010000001         6252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_182377, whole genome shotgun sequence.
ACCESSION   DASTYP010000001 DASTYP010000000
VERSION     DASTYP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182377"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFV59_00005"
     CDS             <1..426
                     /locus_tag="VFV59_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5324248.1"
                     /translation="GAGMPPMAGRMWAYLAICEPPQQTAAEIAARLRASRGSISSMAR
                     LLEHIGLVQRGTRPGDRREYFSIPPGGAHGLMERSAQQLRASREVADAGLALMADRPP
                     ESRQRLQDLRDLYAFFEREWPTILERLRQSDGTGKEPHQ"
     gene            423..1331
                     /locus_tag="VFV59_00010"
     CDS             423..1331
                     /locus_tag="VFV59_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324249.1"
                     /translation="MTAVIETEGLAKYYGKRRGLESLDLEVRSGEIYGFLGPNGAGKT
                     TTIRLLLDLLRPTSGRATVLGTEPHAGGAALRRRIGYLSGDFVVDGGQTGGELLAHLG
                     HLRGGVARERIDALADRLGLDPRVRIRSLSKGNRQKLGLVQAFMHEPELLILDEPTSG
                     LDPILQQTFLDLVEEAKVAGQTVFMSSHVLSEVQRSADRVGIIRDGRMVDVEAVDVLR
                     RRAVRHVELHFDEPVAIEAFAGLPGVSDVTLDGRVLRCRLAGRADALVKMAARYPVES
                     VVVEEPDLEELFLTYYRTDGEATDAA"
     gene            1321..2115
                     /locus_tag="VFV59_00015"
     CDS             1321..2115
                     /locus_tag="VFV59_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5324250.1"
                     /translation="MLRSVFGKSLWDQRRGILGWSLAVAAVGVLYAGFYPSMNNPEMA
                     AAIRAFPQGLLEALGMTDITSPAGYLGSTTYGILGPVLMVIFAGTLGVRAIAGDEEAG
                     RLDVLLAHPVERWAVVVQRAAAMAVALAVAGALLFVAMLVASGPAQFEEVGAGNLAAA
                     TVQLVLLALLFGALGLAAGALTGSRGLAWGVVAGVAVLTYLANTMGPTVDVLAWSQDV
                     SPWFYYSGGRPLANGFQAVDAAVLAATTLVLVVIAVIGFRRRDIAV"
     gene            2168..3085
                     /locus_tag="VFV59_00020"
     CDS             2168..3085
                     /locus_tag="VFV59_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324251.1"
                     /translation="MTSIIETEQLTKFYGTHRGIVDVDLTVTEGEAFGFLGPNGAGKT
                     TMIRTLLDHIRPTRGRARIFGIDTTEDPVAIHRRLGYLPGEFALYDKLTGGQTIEYFA
                     NLRGGVDRAYQQELMDRLEVDPSRRFREYSKGNKQKIGLVVALQHRPELLLLDEPTSG
                     LDPLIQQEFYSVIREAKAEGRTVFMSSHILSEVERTCDRVAIIRDGRLVRVDRVGALR
                     DMAHHTVEIRFTGAVPEAEFRSLPGVSDLVADDHVLRMRVTGGITPVVQAAARHELSD
                     FVSREPTLEETFLAEYGQEPVAEEAAHGR"
     gene            3075..4778
                     /locus_tag="VFV59_00025"
     CDS             3075..4778
                     /locus_tag="VFV59_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324252.1"
                     /translation="MAAELTRTPMRTPAAPTSAGPSLRARLLGLGSVFGKTIRDSRRA
                     MLIVGGISAVLVMVIAAAVVTEFSTPESRREIARVIAAVPPIMQGLAGSGFNVETLGG
                     YIQYKYGGFFPLVTGLWSILALSGTLAAEIRRGSLDFVAAGPVGRRRIAIEKVLAHAT
                     MVFVAMALIMGALWVAGTAFAVLPGDEIPFSAAIGYALWLGLMALAAGSVAFALAPFL
                     GRGAALGIAGGLMFLGFILNGYRTAIPELGQFADLTWFGWTNDHIPLASKYDWASVGV
                     TAAFAVGMLAIGVEAFARRDLGSTIALPMPRMPHALVGLRGPFGRSLSERLPAAISWG
                     VGIGFFGLMMASVGDTFTDQLAQAPGFRAALETLFPDVDIASTGGFLELVFIEFGLIL
                     AGLAGASLVSGWASDETSGRLELLLATPLQRTRWSVASGLGVLAGVVIVMAFTAVGIA
                     IGGATAGGDVGTAVLGSGAVGLYGGALAGVGLAIGGIFGAGVAGTAVVAITILTWLLD
                     FLVPSLDLPSWLQELALSSHMGQPMLGVWDVPGIVLCLVLAVGGVVLGAVTFARRDLG
                     G"
     gene            complement(4775..5608)
                     /gene="nth"
                     /locus_tag="VFV59_00030"
     CDS             complement(4775..5608)
                     /gene="nth"
                     /locus_tag="VFV59_00030"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU5324253.1"
                     /translation="MTAPPKRRRGPSPQKLAADRRKRRRLADVVLERLGERYEHPRWG
                     GARLDAVSELVLTILSQNTADTNSFRAFTALQERYATWEAVLAAPTDELEDVIRPGGL
                     APTKSRRIQHVLAEVHETTNGTWDMSFLGTWPLQHARDWLISLPGIGRKTAAIILLFN
                     FGRPQLPVDTHVHRVATRLGMLPPRTPLERAHDLLEEVLQPEEMYPFHVELIRHGRDT
                     CRAPRPICGLCPLTDVCAYYADAIRGRAPMAPVPRTPRSSEAEPRYHDELIHGWMPPD
                     A"
     gene            complement(5605..5949)
                     /locus_tag="VFV59_00035"
     CDS             complement(5605..5949)
                     /locus_tag="VFV59_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324254.1"
                     /translation="MVPVVRVLVGVASVAALLIGLVVLVSRSSDAPGGLWLMVLGAVG
                     LIGVTFERLRYRSETAERSGEPAGAAGVESGPPDPRFRPTEERFVDPTTRARLRVWVD
                     PASGERRYRLDE"
     gene            complement(5958..>6252)
                     /locus_tag="VFV59_00040"
     CDS             complement(5958..>6252)
                     /locus_tag="VFV59_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU5324255.1"
                     /translation="KGQAFGLVVRSRGLAGNISAGLRSLIGGEIHEYTELLEDTRRQA
                     LDRLVQNASLMGANAVISMRFDSSELNNSMSEIVAYGTAVVIEPDASAPQPVE"
BASE COUNT          816 a         2185 c         2285 g          966 t
ORIGIN      
        1 ggcgcgggca tgccgccgat ggccgggcgg atgtgggcct acctcgcgat ttgcgagcca
       61 cctcaacaga cggcggccga gattgccgcg cggctcaggg cgagccgcgg atccatcagc
      121 tccatggcgc gcctgctcga gcacatcgga ctcgtgcagc gcggtaccag gccgggtgac
      181 cgacgcgagt acttcagcat cccgcccggc ggtgcccacg gcctcatgga gcgcagcgcg
      241 cagcagctgc gcgcttcccg cgaggtggcc gatgcgggcc tcgcgctgat ggcggaccgg
      301 ccgcccgagt cgcgtcaacg gctccaggac ctgcgcgacc tctacgcgtt cttcgagcgc
      361 gagtggccga cgatcctcga gcgcctccgt caatccgatg gaaccggaaa ggaaccacac
      421 caatgaccgc ggtcatcgag accgagggcc tcgccaagta ctacggcaag cgccgtgggc
      481 tcgagagcct ggacctggag gtccgatcgg gcgagatcta cggcttcctc ggcccgaacg
      541 gggccgggaa gacgaccacc atccggctgc tcctcgacct gctgcgaccg accagtgggc
      601 gcgccacggt cctgggaacc gagcctcacg ccggcggcgc cgcgctgcgg cggcgcatcg
      661 gctacctgtc cggcgacttc gtcgtcgacg gtggccagac cgggggtgag ctgctggccc
      721 atctcggcca cctgcgcggt ggcgtggcca gggagcggat cgacgcgctg gcggatcggc
      781 tgggcctcga cccgcgcgtg cgcatccggt cgctctcgaa gggtaaccgg cagaagctcg
      841 gcctggtgca ggcgttcatg cacgagccgg agctgctgat tctggacgag cccacgtccg
      901 gcctcgaccc gatcctgcag cagaccttcc tcgatctggt cgaggaggcc aaggtggccg
      961 ggcagaccgt cttcatgtcg tcgcacgtcc tctcggaggt gcagcgctcg gccgaccggg
     1021 tgggcatcat ccgtgacggg cgcatggtgg acgtggaggc cgtcgacgtc ctgcgacggc
     1081 gcgccgtgcg ccacgtcgag ctgcacttcg acgagcccgt cgccatcgag gcgttcgccg
     1141 gccttccggg cgtcagcgac gtgacgctgg acggccgcgt gctgcgctgc cgcctggcgg
     1201 gacgggccga cgcgctggtg aagatggcgg cccgctaccc ggtcgagagc gtcgtggtcg
     1261 aggagccgga cctcgaggag ctgttcctca cctactaccg gactgacggg gaggccaccg
     1321 atgctgcgta gcgtgttcgg caagtcgctg tgggaccagc gccgcggcat cctcggctgg
     1381 tcgctcgccg tcgcggcggt gggtgtcctg tacgccggct tctatccgtc gatgaacaac
     1441 ccggagatgg cggccgccat ccgcgccttc ccgcagggcc tgctcgaggc cctcggcatg
     1501 accgacatca ccagtccggc cggctacctg ggcagcacga cgtacggcat cctcggcccg
     1561 gtgctgatgg tgatcttcgc gggcaccctg ggcgtgcggg ccatcgccgg cgacgaggag
     1621 gcgggccggc tcgacgtgct gctcgcccac ccggtcgagc gatgggccgt cgtcgttcag
     1681 cgagcggccg ccatggcggt cgcgctcgcc gtggcgggcg cgctgctctt tgtcgcgatg
     1741 ctcgtcgcgt ccgggccggc ccagttcgag gaggtcggcg ccggcaacct cgccgcggcg
     1801 acggtgcagc tggtcctgct ggcgctgctg ttcggcgcgc tcggcctcgc cgccggcgcc
     1861 ctcaccggca gccgcgggct ggcctggggc gtcgtggccg gcgtggcggt gctcacctac
     1921 ctggcgaaca cgatgggccc gaccgtcgac gtcctcgcct ggagccagga cgtctccccg
     1981 tggttctact actccggtgg ccggccactg gcgaacggct tccaggccgt cgacgccgcc
     2041 gtgctggcgg cgaccacgct cgtgctggtc gtcatcgccg tcatcggctt ccggcggcgc
     2101 gatatcgccg tctgagcagg accgatacgc cgatgtgtct gcatcggcgg ggaaaggaca
     2161 cgcattcgtg acctcgatca tcgaaaccga gcagctgacc aagttctacg ggacgcaccg
     2221 cgggatcgtg gacgtcgacc tcaccgtcac cgagggcgag gccttcggct tcctcgggcc
     2281 caacggcgcc gggaagacga ccatgatccg gacgctgctc gaccacatcc gcccgacccg
     2341 gggtcgggcg cgcatcttcg gcatcgacac gaccgaggac cccgtcgcca tccatcggcg
     2401 cctcggctac ctgccgggtg agttcgcgct gtacgacaag ctcaccggcg gtcagacgat
     2461 cgagtacttc gccaacctgc gtggcggggt ggaccgggcc tatcagcagg agctgatgga
     2521 tcggctggag gtggacccga gccgtcgatt tcgcgagtac tcgaagggca acaagcagaa
     2581 gatcgggctg gtggtggcgc tgcagcatcg gccggagctg ctgctgctcg acgagccgac
     2641 gtcgggcctg gaccccctga tccagcagga gttctactcg gtcatccggg aggcgaaggc
     2701 cgagggccgg accgtgttca tgtccagcca catcctgtcg gaggtcgagc gcacctgcga
     2761 ccgggttgcg atcatccgcg acgggaggct ggttcgcgtc gaccgcgtcg gcgccctgcg
     2821 ggacatggcg caccacacgg tggagatccg cttcacgggg gcggtgccgg aggccgaatt
     2881 ccggtcactt cccggcgtca gcgacctcgt cgccgacgac cacgtcctgc ggatgcgcgt
     2941 gaccggtggc atcacgccgg tcgtccaggc cgcggctcgc cacgagttga gcgacttcgt
     3001 cagccgcgag ccgacgctcg aggagacctt cctggccgag tacggccagg agccggtcgc
     3061 cgaggaggcc gcgcatggcc gctgagctga cccgcacacc gatgcggacg ccggccgcgc
     3121 cgacgtccgc agggccgtcg ctgcgggcgc gcctgctggg actgggcagc gtcttcggca
     3181 agacgatccg cgactcgcgg cgcgccatgc tcatcgtcgg cggcatcagc gcggtcctgg
     3241 tcatggtcat cgcggccgcc gtcgtcaccg agttctccac cccggagtcc cgcagggaga
     3301 tcgcccgcgt catcgccgcc gtcccgccca tcatgcaagg gctggcgggg agcggcttca
     3361 acgtcgagac cctgggcggc tacatccagt acaagtacgg cggcttcttc ccgctggtca
     3421 ccggcctgtg gtcgatcctc gccctgtccg ggacgctggc cgccgagatc cggcgtggct
     3481 cgctcgactt cgtcgcggcc gggccggtcg gtcggcgacg aatcgcgatc gagaaggtgc
     3541 tcgcccacgc caccatggtg ttcgtggcga tggccctgat catgggcgcg ctgtgggtgg
     3601 ccggcaccgc gttcgccgtc ctgcccggcg acgagatccc gttctcagcc gccatcggct
     3661 acgcgctgtg gctcgggttg atggcgctgg ccgccggttc cgtcgccttc gcgctggccc
     3721 cgttcctggg ccgcggtgcc gcgctcggca tcgccggagg cctgatgttc ctcggcttca
     3781 tcctgaacgg ctaccggacg gccatcccgg agctcggcca gttcgcggac ctgacgtggt
     3841 tcggctggac caacgaccac atcccgctcg cgtcgaagta cgactgggcg tccgtcggcg
     3901 tgacggcggc cttcgcggtc gggatgctcg ccatcggcgt cgaggcgttc gcgcgccggg
     3961 acctcggcag cacgatcgcc ctgccgatgc cacgcatgcc gcacgccctg gtcgggctgc
     4021 gcggtccctt cggtcgcagc ctgagcgagc gtctgccggc ggcgatctcg tggggcgtcg
     4081 gcatcggctt cttcgggctc atgatggcct cggtgggtga caccttcacc gaccagctgg
     4141 cccaggcgcc gggcttccgg gccgcgctcg agacgctctt ccccgacgtt gacatcgcct
     4201 ccaccggtgg cttcctggag ctcgtgttca tcgagttcgg gctgatcctc gccgggctcg
     4261 ccggagcgtc gctcgtgtcc ggctgggcgt cggacgagac gtccggacgc ctggagctgc
     4321 tcctggcgac accgctccag cgcacacggt ggtccgtcgc gagcggactg ggcgtgctgg
     4381 ccggcgtggt gatcgtgatg gccttcacgg cggtcggcat cgccatcggc ggcgcgaccg
     4441 ccggcggcga cgtcggaacg gcggtcctgg gcagcggcgc ggtcgggctg tacggcggcg
     4501 cgctggccgg ggtcggcctg gccatcggcg gcatcttcgg cgccggcgtc gccgggaccg
     4561 cggtcgtcgc catcaccatc ctgacctggc tgctcgactt cctggtgccc agcctcgacc
     4621 tgccgtcctg gctgcaggag ctggcgctgt cgtcgcacat gggccagccg atgctcggcg
     4681 tgtgggacgt ccccggcatc gtgctgtgct tggtcctcgc cgtcggcggg gtcgtgctcg
     4741 gggcggtcac gttcgcgagg cgcgacctgg gaggctaggc gtccggtggc atccagccgt
     4801 ggatgagctc gtcgtggtac cgcggctccg cctcggagga tcgaggcgtc cgcgggaccg
     4861 gcgccattgg cgcgcggccg cggatggcat cggcgtagta ggcgcacacg tcggtcagcg
     4921 ggcagaggcc gcagatggga cgaggagcgc ggcaggtgtc acgcccgtgg cggatgagct
     4981 cgacgtggaa cgggtacatc tcctccggct gcagcacctc ctccagcagg tcgtgggcgc
     5041 gctcgagcgg tgtacgcggc ggcagcatgc cgagccgcgt cgcgacgcgg tggacgtggg
     5101 tgtcgacggg cagctgcggc cggccgaagt tgaagaggag gatgatcgcc gccgtcttgc
     5161 ggccgatccc cggcagcgag atcagccagt cgcgggcgtg ctgaagcggc cacgtcccga
     5221 ggaacgacat gtcccacgtg ccgttggtcg tctcgtgcac ctcggccagc acgtgctgga
     5281 tccgcctcga cttggtgggc gcgaggccgc ccggccggat gacgtcctcc agctcatcgg
     5341 tcggcgcggc cagcaccgcc tcccaggtcg cgtagcgctc ctgcagcgcg gtgaaggcgc
     5401 ggaacgagtt ggtgtccgcg gtgttctgcg acaggatggt gaggacgagc tcgctgaccg
     5461 cgtccagccg agcgccaccc cagcgcgggt gctcgtagcg ctcgccgagc cgctccagga
     5521 ccacgtcggc caggcggcgg cgcttccgcc gatccgccgc gagcttctgc gggctcggcc
     5581 cgcgacggcg cttcgggggg gcggtcactc gtcgaggcgg tagcgtcgct cgccgctcgc
     5641 cgggtcgacc cagacgcgca gtcgggcgcg cgtggtcggg tcgacgaagc gttcctcggt
     5701 ggggcggaac cgtgggtcgg gtggcccgct ctcgacgccg gcggcaccgg ccggctcgcc
     5761 ggatcgctcg gccgtctcgg atcggtagcg caggcgctcg aaggtcacgc cgatcagtcc
     5821 gaccgcgccg aggaccatga gccacaggcc accgggcgca tcggacgaac gcgacacgag
     5881 cacgaccagg ccgatcagca gggcggcgac ggaggcgacg ccgaccagga cacggacgac
     5941 ggggaccacg gcgtcgccta ctcgaccggc tgcggcgcgg aggcgtccgg ctcgatgacc
     6001 acggccgtgc cgtaggcgac gatctcgctc atcgagttgt tcagctcgga gctgtcgaac
     6061 cgcatgctga tgacggcatt ggcgcccatc aggctggcgt tctggacgag gcgatccagt
     6121 gcctggcggc gggtgtcttc cagcagctcg gtgtactcgt ggatctcgcc gccgatcagc
     6181 gagcgcaggc cggcactgat gttgccggcc agcccgcggc tgcggacgac gaggccgaag
     6241 gcctgcccct tc
//
LOCUS       DASTYP010000002        11998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1639616, whole genome shotgun sequence.
ACCESSION   DASTYP010000002 DASTYP010000000
VERSION     DASTYP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11998
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639616"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..141
                     /locus_tag="VFV59_00045"
     tRNA            66..141
                     /locus_tag="VFV59_00045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Arg,seq:cct)
     gene            complement(189..1193)
                     /locus_tag="VFV59_00050"
     CDS             complement(189..1193)
                     /locus_tag="VFV59_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324256.1"
                     /translation="MRRTVAVIIAVAALVAACGGGATSPAISSSPTEMHSPTALVTPT
                     AQPTPEPSFVEPPGTATPSAARVRQWAADALAFGETFDAAYADPEAVFMAFAEDARTR
                     DPSNGDYLFDGKKAIVSAWEGLVRGWPDLDAHPTAAYVSSDGALIATDVDNLAPDRLH
                     ELRRYRWNGNRVTEFELWYRVDQLGGCQPGTCEADARQLADRYLAAWSGDTADAVAAM
                     YRDDATLSDTLIGVDAVGSAAIAETAEQRFGTAAPACQAIDLYGLTNGGIQIIGLAIH
                     AQCVPSGAPSSAVESVFLLRFGSYAGDGFDVDADGLITSEEVYHDADSLAASGLLGDE
                     "
     gene            1417..3438
                     /locus_tag="VFV59_00055"
     CDS             1417..3438
                     /locus_tag="VFV59_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5324257.1"
                     /translation="MTDSEHGGHAAHDKHAGHDPEVFRRQFWIVLLLTIPVVVWSHDV
                     QEWLGYTAPAFPGSDWLTAVLGTIVFVYGGRVFLNGARTELADRQPGMMTLISLAIVV
                     AFVTSWAATLGFFEVDVWWELATLITVMSLGHWLEMRSIMQARGALAALAELLPDEAE
                     RVTDGGTETVKTADLRRGDVVLVRPGGRVPADGEVAEGIADVDESMITGESRPVSKEP
                     GDRVVAGTVAAGGSLRVAVTAVGEETALSGIMRLVAQAQASNSRAQALADRAAAILFY
                     IALAAGTLTLIAWTVLGDPEQALIRTATVLVIACPHALGLAIPLVIAISTTLGARNGL
                     LVKDRLALERARELDVVIFDKTGTLTRGEPVLAGVASVDGVRDDELLALAAAVEADSE
                     HPLARAIVDGARARQVQPLTASGFENLAGRGAKARIGGSEVHVGGPRLLQELDLQPPQ
                     ALSSETQGWADAGRTVMHVVRDGTVIGAVSVEDEIRPESVEAVRHLHEMGLRVAMITG
                     DAQSVAENVAQRIGIDEVAAQVLPADKADAVKRFQTGGRRVAMVGDGVNDAPALAQAD
                     VGIAIGAGTDVAVEAAGIVLVRDDPRDVAGAIELSRATYRKMIQNLAWATGYNALAIP
                     VAAGVLAPIGFVLPMSVGAVAMSLSTIIVAANAQLLRRLRLRPSEAQAP"
     gene            complement(3457..3900)
                     /locus_tag="VFV59_00060"
     CDS             complement(3457..3900)
                     /locus_tag="VFV59_00060"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEU5324258.1"
                     /translation="MRLLRALILVSLGVFAGFAGAAAALRGWLAWRGAEPRSEDELEV
                     MAIFDGVELQNRSAALRGGRVLAWFGGVTLDLSQATLAPGAELDLRALFGGLRVIVPS
                     GCRVDAETRSILGGVAVSAPTPSDPDAPVLLVRAIGVFGGISVEG"
     gene            complement(3947..4678)
                     /locus_tag="VFV59_00065"
     CDS             complement(3947..4678)
                     /locus_tag="VFV59_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5324259.1"
                     /translation="MTDVSILTAIAAGALSFLSPCVLALLPVYLAFLGDAAALPALPA
                     PAAGGPGARQPAMTAVLPQALLFVAGFSLLFIVVFTSVAAVGAPLLRIPEVRQAAGVA
                     VIVLGVLTTGVFGPVLDRFRIGVSADALPAGRSARALALGGLVAIGWTPCIGPVLGAI
                     FTMGASSGSVPVVALLLAAYSIGLAIPFLMAAVAFPRVRPLVEWLRRHHRAVQVASGM
                     LIVAIGVLIFFNAFARLATLFTFAL"
     gene            complement(4710..6242)
                     /locus_tag="VFV59_00070"
     CDS             complement(4710..6242)
                     /locus_tag="VFV59_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324260.1"
                     /translation="MSRRLAAGAATFLVGAAWLLAPSAATAHALGGTFQLPVPLWLYL
                     AGAAFAVAASFVVTAILARERAERAGYPTRPIPEGVAVAARYALRVIGLVWWYGAIVV
                     GFLVGDISPLPAVLLWIGIWVGLPIAAVLIGNPWPSLSPFRTTWAALEWVGRWLGARS
                     LDLGLPYPRTLGRWPAFVLLAAGIWIELILPGREVAFAVALLMTAYTVLTLLGMLAFG
                     QIAWLRNAELLEVELAWFGRVGPIGRRSVSVELCGGCDDGCEVERCIDCPECSTAADD
                     AERAPVLRPWVAGLTDVTRAGWSDAAFIVLALAGVSFDGLQETAFGAWLLDVFLPPVQ
                     AVLGTTATTFLLVDTLAFASVFAAFFAAFTLVVTVTHRIGDHGRTSVGSTAGVYAATL
                     LPIAGGYLIAHYLTLVIQGAVWLPELVADPLNSLAPELSWIPMSGVWYLSVGSIVAGH
                     VAAIVLAHRLALRDAADRAVRAGLPMVGLMVGYTILSLWIIAQPIVVEPGGGGAGAGF
                     LP"
     gene            complement(6232..6615)
                     /locus_tag="VFV59_00075"
     CDS             complement(6232..6615)
                     /locus_tag="VFV59_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324261.1"
                     /translation="MSRRIPLLLLLVTAAALAGCVTDNRPAESACAAASVQLDLTLTA
                     EALDPSDPGVCRGQEVTMVVHSQVDGVIHLHGYDEQVDATPVSAGEDTTLEFTAATSG
                     QFPIELHAEDDPRGVEVGIFTVHEP"
     gene            complement(6612..7151)
                     /locus_tag="VFV59_00080"
     CDS             complement(6612..7151)
                     /locus_tag="VFV59_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEU5324262.1"
                     /translation="MRLSRLLASVTAAVALLALPAAVMAHADLESSTPEAGADVESAP
                     SEVVLTFSGELGPDSAFVVTDAAGGTVGEGGLDLDVAVRNVLSGSVDITQPGIYTVSW
                     SAVSIDGHAEEGSFEFGFQSDAAAAAGAPPDDAGSPDTAMPASGAPGAPGALSAAGLA
                     LLAAGMVVAGRRALVVHRA"
     gene            complement(7169..9463)
                     /locus_tag="VFV59_00085"
     CDS             complement(7169..9463)
                     /locus_tag="VFV59_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEU5324263.1"
                     /translation="MPTIAHRTTFHLRRLLAATLLVVIGTVARPAGAWAHAELLSSDP
                     AANATLTESPAAVVLTFTEAVDPATVSVELLDEAQDAVAGVGDPTVDGGVTVRASLPT
                     LEPGVYTVSYQVVSSIDGHATAGIFAFLVDPTGTEAAPTGSGSETSPSVDVTTILARW
                     LGLLGSLIALGSLLLWWTAGRRALTDLGLAERRPPWGTVASAASVAVAGTALYLWLAA
                     RPIVDAAARSGVAPPSTGIPLDVAGPFGWTPFSIAMRVAVAALLATSVVAVLGAVRGR
                     GGDGRALPLAGTILLLVALGAMSQAAHAAAAGGPLFGALDWVHLVAVAAWLGALPAAF
                     VLAARARRFRPALASILRRHGAVALVAGPVVVLTGVANSPLVLGTPRDVVGSDYGNLV
                     LAKAALVSVALGLGAVNHFALRGRGRAATATLVGAELVVAALAVAAAATMVAIQPAAA
                     RAPRLVAAEVNPAHLYGAAGPSTVHAAVNLPAPGNQQYEVAVNDAETGSPRLDVQKVF
                     LDFTPPAGTDLPSERIEMTPSGTVPGLYTARGAYTPLDGTWRLDVVVRRHGALDESVS
                     FALPVSSPGPAEEAPPEDSGIGVPAPLAALWPILPPGPLAWLLVPAALLLAWFLGRDP
                     RGQPLRTVLRAALVVVALAAGLGAGSRDLVAAANAPPSAVSASPSASPPAGAESVARG
                     ERLYQANCLACHGRDGLGDGPVRTLPGAGDLPAAVRGASAAELDYRMANGVAGTAMPA
                     FIGQLTPGERQDLVAYLRSRWGGP"
     gene            9618..10082
                     /locus_tag="VFV59_00090"
     CDS             9618..10082
                     /locus_tag="VFV59_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5324264.1"
                     /translation="MASVVESIDVKVPVRTAYDQWTQFEEFPRFMEGVKSVTQMGDTN
                     LHWVAEVGGKEKEWDAKITEQKPDERVAWTNTSGAENAGVVTFHRLDDETTKVTLQMD
                     VDPEGMVENVGTALGFLDRRVKGDLERFKEFIENRGVETGAWRGEVEAGAQR"
     gene            10079..10477
                     /locus_tag="VFV59_00095"
     CDS             10079..10477
                     /locus_tag="VFV59_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324265.1"
                     /translation="MTDRESTGGGGLTGATGDLTPADTDAAFDPGERREFGDPAVQAE
                     MTAAQGARADAQQGQIGRPEDQPVGGPTNLADRDMGYGSEHGLDPEDPAYRMESHPPA
                     ARAATDESEDASDRSTRLGGDELLEEEPHF"
     gene            complement(10498..11361)
                     /locus_tag="VFV59_00100"
     CDS             complement(10498..11361)
                     /locus_tag="VFV59_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU5324266.1"
                     /translation="MRPIWKGAISFGMVTIPVKLYAATESKSIQFRLLCKEHEAPIEE
                     KRVCTEGGEELAWDDLVRGFQVSKNEYVILDPEEIDAAKPESSTTIDIGDFVEAVEID
                     PVYFEKSYFLEPTDVGGKAFSLLKKALEETERVALARVTIRTRERLATLRAYDDTLIL
                     ETMFWPDEIRSTGSLDLPEGAEAKVRAKELDMARSLVESLADRFRPDDYTDDYRVALE
                     ELIEQKMRGEVRNAKRRRPEPKVIDLMEALRASVSEAKGARTKAGGKSAPTKETKNRP
                     ARKRAARRSAA"
     gene            complement(11384..11635)
                     /locus_tag="VFV59_00105"
     CDS             complement(11384..11635)
                     /locus_tag="VFV59_00105"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEU5324267.1"
                     /translation="MSELDAKDRDKLDRDEFAYVDSDGGEHLPINDESHVRNAIARYN
                     QTEFESKAAKERARKKILSAADKYGIEVDEDDNVRQSVG"
     gene            11670..>11998
                     /locus_tag="VFV59_00110"
     CDS             11670..>11998
                     /locus_tag="VFV59_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU5324268.1"
                     /translation="MARRVPIRGSVSVAVRVGTSGWQYRHWAGDFYPAELPTSEWLAY
                     YVERFDTVELNASFYRLPRAEAFAAWGRRVPDGFRFAVKASRYLTHVKRLKDPDEPLD
                     RFWTRVRR"
BASE COUNT         1828 a         4220 c         4410 g         1540 t
ORIGIN      
        1 gcacggacgt agagggtcgg acgcgcgggc atgggccgat gatacggtag gcttcgttcg
       61 gcgccgcccc cgtagctcag tggatagagc gccgccgtcc taaggcgggc gtcgggggtt
      121 cgaatccctc cgggggcgcc atggcaccgc catcgtcgat gcgaggcagc cacgctgagg
      181 cgaccggcct attcatcgcc gagcagcccc gatgccgcca gtgagtccgc atcgtggtag
      241 acctcttcgc ttgtgatcag gccgtctgcg tcgacgtcga acccgtcgcc ggcgtatgag
      301 ccgaagcgga ggaggaagac actttccacc gcgctggaag gcgcgccgct gggaacgcat
      361 tgcgcatgga tcgccagacc gatgatctgt atgccaccgt tggtcaggcc gtacaggtcg
      421 atggcctggc acgccggcgc ggccgttccg aaccgctgct cggccgtctc cgcaatggcg
      481 gccgatccga cggcatccac gccgatcagg gtgtccgaga gcgttgcgtc gtcgcggtac
      541 atcgccgcaa cggcgtcagc ggtgtcaccc gaccacgccg cgaggtaccg gtcggccagc
      601 tgtcgggcgt ccgcctcgca cgtccccggt tggcagccgc ccagctgatc gacgcggtac
      661 caaagctcaa actccgtgac gcgattgccg ttccaccggt agcggcgaag ttcgtgcagc
      721 ctgtcggggg ccaggttgtc gacgtccgtg gcgatcagcg caccgtcgga ggagacgtag
      781 gccgcggtcg gatgcgcgtc gaggtcgggc cagcctcgca ccagcccttc ccaggcactg
      841 acgatggcct tcttcccgtc gaagaggtag tcgccgttcg atgggtcacg cgtcctggcg
      901 tcttccgcga acgccatgaa gacggcttcg gggtcggcat aggcggcatc gaacgtttct
      961 ccgaaggcaa gcgcgtcggc cgcccattga cggacccgcg cggccgaggg ggtggccgtc
     1021 ccgggaggct cgacgaacga cggctccggg gtcggctgcg ccgttggtgt gacgagcgcc
     1081 gtcggactgt gcatctcggt gggcgacgac gagattgcgg gcgacgttgc tccgccgccg
     1141 caggcggcca ccagagccgc gacagcgatg atgaccgcga cggtgcgacg cacgatccca
     1201 ggcctcaagg cgtggagaca caagcgtcgc tgcgcgcggt tgaggcgtcg agtgccgatc
     1261 ggcccgaggg tcggcgttcg cgagataggc cgcggggtac catcggccga tgagccgccg
     1321 cctggtctgg aagtcgccgt gacctcacca cgcgacgcgc acggagggca cgggactgat
     1381 cagtccgcgg cgccggcaga tccgcaccat gcgcccctga cggacagcga gcatggcggc
     1441 cacgccgcac acgacaagca tgccggccac gaccctgagg tcttccgacg gcagttctgg
     1501 atcgtgctgc tcctcaccat cccggtggtg gtgtggagtc acgacgtgca ggagtggctc
     1561 ggctacacgg cgccagcgtt ccccggctcg gattggctga cggcggtcct cggcaccatc
     1621 gtcttcgtct acggcggtcg agtcttcctg aacggcgccc ggacggagct cgccgatcgt
     1681 cagcccggga tgatgacgct gatcagcctg gccatcgtcg tggccttcgt cacgtcgtgg
     1741 gcggcgaccc tcggcttctt cgaggtcgac gtgtggtggg agctcgccac gctgatcacg
     1801 gtcatgagcc tgggtcactg gctcgagatg cgctccatca tgcaggcccg cggcgcgctg
     1861 gccgcgctgg cggagctcct ccccgacgag gcggagcgcg tcacggacgg cgggaccgag
     1921 acggtcaaga cggccgacct gcgcaggggt gacgtggtgc tggttcggcc gggtggtcgg
     1981 gtgccggccg acggcgaggt ggcggagggc atcgccgacg tggacgagtc catgatcacg
     2041 ggcgagtcgc ggccggtgtc caaggagccg ggcgaccggg tggtggcggg cacggttgcg
     2101 gcgggcggca gcctgcgagt tgcggtcacc gccgttggag aagagacggc gctctccggc
     2161 atcatgcggc tggtggcgca ggcccaggcg tcgaactcgc gggcccaggc gctggccgac
     2221 cgagccgcgg cgatcctctt ctacatcgcg ctcgcggccg ggaccctgac gctgatcgcg
     2281 tggacggtcc tcggcgaccc cgagcaggcg ctgatccgga cggccaccgt cctggtcatc
     2341 gcctgcccgc acgcgctggg cctcgcgatc cccctcgtga tcgcgatcag cacgacgctg
     2401 ggtgcgcgca acgggctgct ggtcaaggat cggctcgcgc tggaacgtgc ccgggagctg
     2461 gacgtcgtca tcttcgacaa gaccggcacc ttgacccgcg gtgagccggt gctcgccggg
     2521 gtcgcatccg tcgacggggt gagggacgac gagctcctcg cgctggctgc ggcggtcgag
     2581 gcggacagcg agcacccgct ggcccgggcg atcgtggacg gagcgcgcgc ccgccaggtc
     2641 cagcccttga ccgcttccgg gttcgagaac ctggccggtc gcggggccaa ggcgcgcatc
     2701 ggcgggagcg aggtgcacgt cggcgggccg cggctcctgc aggagctgga cctgcagccg
     2761 ccgcaggcgc tctcgtccga gacgcaggga tgggccgatg cagggaggac ggtgatgcac
     2821 gtcgtgcgcg acggcaccgt catcggtgcg gtgagcgtgg aggacgagat ccgaccggaa
     2881 tccgtcgagg cggttcgcca cctccacgag atggggctgc gggttgcgat gatcaccggc
     2941 gacgcgcagt cggtggccga gaacgtcgcg cagcgcatcg ggatcgacga ggtggccgcg
     3001 caggtgctgc cggcggacaa ggccgatgcg gtcaagcgct tccagacagg tgggcggcgg
     3061 gtggcgatgg tcggcgacgg cgtcaacgac gcgccagcgc tggcccaggc cgatgtcggg
     3121 atcgcgatcg gcgcggggac cgatgtcgcc gtcgaggcgg ccggcatcgt gctggtccgc
     3181 gacgacccgc gggacgtggc cggcgcgatc gagctcagcc gcgcgaccta tcggaagatg
     3241 atccagaacc tggcctgggc gacgggctac aacgccctcg ccattccggt ggcggccggc
     3301 gtcctggcac ccatcggctt cgtgcttccg atgagcgtcg gcgcggtggc catgagcctc
     3361 tcgaccatca tcgtggcggc caacgcccag ctcctgcgcc gtctgcggct gcgaccctcc
     3421 gaagcgcagg cgccgtgacg gctgcccgcc tcgcgctcag ccctcgacgg agatgccgcc
     3481 gaacacgccg atggcgcgca cgaggaggac cggcgcgtcg ggatccgacg gcgtcggggc
     3541 gctgaccgcg acgccgccga ggatcgagcg cgtctccgca tcgacgcggc agccggacgg
     3601 aacgatcacg cgcaggccgc cgaagagcgc gcggaggtcg agctccgcgc ccggcgccag
     3661 cgtcgcctgg ctcaggtcga gggtgacgcc gccgaaccag gcgaggacgc ggccaccgcg
     3721 cagggcggcg gaccggttct ggagctcgac tccgtcgaag atggccatca cctcgagctc
     3781 gtcctcggac cgcggctccg cgccgcgcca ggcgagccag ccgcgcagcg cggcagcggc
     3841 gccggcgaac ccggcgaaga caccgaggct gaccaggatc agggctcgga gcagacgcat
     3901 catccggtca gcctagcacc gcgccgaccc tcgtcccgca gacccgctac agcgcgaacg
     3961 tgaacagggt tgccagacgc gcgaaggcgt tgaagaagat gagcacgccg atggcgacga
     4021 tgagcatccc ggatgcgacc tgcaccgccc gatggtggcg ccgcagccac tcgaccagcg
     4081 gccgcacgcg tgggaaggcc acggcggcca tcaggaacgg gatggccagc ccgatcgagt
     4141 aggcggccag cagcagcgcg acgaccggga ccgatccgct cgaggcgccc atggtgaaga
     4201 tggcgcccag gaccggaccg atgcacggcg tccagccgat cgcgaccagg ccgcccaggg
     4261 ccagcgcccg tgccgagcga ccggccggga gcgcatcggc cgagacgccg atccggaatc
     4321 ggtcgaggac cggcccgaag accccggtgg tcaggacgcc gagcacgatc accgccacgc
     4381 cggccgcctg gcgcacctcg gggatgcgga gcaggggagc gcccacggcc gccaccgagg
     4441 tgaagacgac gatgaacagc aggctgaacc cggcgacgaa gagcagggcc tgcggcagga
     4501 cggcggtcat cgccggctgc cgtgcgccgg gcccgccagc ggccggagcg ggcagcgcgg
     4561 ggagcgcggc cgcgtcgccg aggaaggcca ggtagaccgg cagcagggcg agcacgcacg
     4621 gcgacaggaa cgagagggcg ccggccgcga tggctgtcag gatgctgacg tcggtcacgc
     4681 tgcgagccta gccgacgccg acgcgagcgt caggggagga agccggcgcc cgcgccaccg
     4741 ccgccgggct cgaccacgat cggctgcgcg atgatccaga gcgacaggat cgtgtagccg
     4801 accatcagcc ccaccatcgg caggccggcc cggacggcgc ggtccgccgc gtcgcgcagg
     4861 gcgaggcgat gggcgaggac gatggccgcc acgtggccgg cgacgatgga gcccacgctc
     4921 aggtaccaga cgccgctcat cggaatccag ctcagctcgg gcgccaggct gttcagcggg
     4981 tcggccacca gctccggcag ccatacggcc ccctggatga ccagggtcag gtagtgcgcg
     5041 atgaggtaac cacccgcgat cggcagcaac gtggccgcgt acaccccggc ggtggacccg
     5101 acggaggtgc gcccgtggtc gccgatgcgg tgcgtcaccg tgacgaccag cgtgaaggcg
     5161 gcgaagaagg cggcgaagac cgatgcgaag gcgagcgtgt cgaccagcag gaaggtggtc
     5221 gccgtggtcc ccagcaccgc ctggaccggt ggcaggaaga cgtcgagcag ccacgccccg
     5281 aacgccgtct cctgcaggcc gtcgaagctg accccggcca gggccagcac gatgaacgcg
     5341 gcgtccgacc agccggcccg cgtgacgtcg gtgaggccag ccacccacgg ccgcagcacg
     5401 ggcgcgcgct ccgcatcgtc ggccgcggtg gagcactccg ggcagtcgat acagcgctcg
     5461 acctcgcacc cgtcgtcgca tcccccgcac agctccacgc tgaccgaacg gcggccgatg
     5521 ggccccacgc ggccgaacca cgcgagctcg acctcgagta gctccgcgtt gcgcagccag
     5581 gcgatctgcc cgaacgccag catgccgagc agcgtgagca cggtgtaggc ggtcatgagc
     5641 agggcgacgg cgaacgccac ctcgcggccg gggaggatca gctcgatcca gatgccggcg
     5701 gccaggagca cgaacgccgg ccagcgtccc aacgtgcgcg ggtaggggag tcccaggtcg
     5761 aggcttcggg cccccaacca ccgccccacc cactcgagcg ccgcccaggt cgtccggaac
     5821 gggctcagtg atggccacgg attgccgatc aggaccgcgg cgatcgggag tcccacccag
     5881 atgccgatcc acagcagcac cgccggcagc ggcgagatgt cgccgaccag gaatccgacc
     5941 acgatcgcgc cgtaccacca gaccaacccg atgacgcgga gggcgtagcg ggcggccact
     6001 gccacgccct ccggaatcgg tcgcgtcggg taccccgcac gctccgcacg ctcccgggcc
     6061 aggatcgcgg tgacgacgaa cgaggccgcc accgcgaagg ccgcgccggc caggtacagc
     6121 cacaggggga ccggcagctg gaaggtcccg cccagcgcgt gcgcggtcgc cgccgacggt
     6181 gccagcagcc aggccgcgcc gacgaggaag gtcgccgcgc cggcggcgag cctacggctc
     6241 atgcacggtg aagatgccga cctcgacgcc gcgcggatcg tcctcggcgt gcagctcgat
     6301 cgggaactgg ccgctggtcg cggcggtgaa ctccagcgtg gtgtcctcgc cggcgctcac
     6361 cggcgtggcg tcgacctgct cgtcgtagcc gtggaggtgg atgacgccgt cgacctgcga
     6421 gtggacgacc atggtcacct cctggccgcg gcacacgccc ggatccgacg ggtcgagcgc
     6481 ttcggcggtc agcgtcaggt cgagctgcac cgatgccgcg gcgcacgcgc tctcggccgg
     6541 ccggttgtcg gtgacgcaac ccgcgagggc cgcggcggtt accagcagga gcagcagtgg
     6601 gatgcggcgg ctcatgcccg atgcaccacc agcgcgcgcc ggccggcgac gaccatcccc
     6661 gcggccagca gcgccaggcc ggccgcgctc aacgcgccag gcgcaccagg tgcgcccgac
     6721 gccggcatgg ccgtgtccgg cgatccggcg tcgtcggggg gtgcaccggc cgcggcggcc
     6781 gcatcggact ggaagccgaa ctcgaagctg ccctcctcgg cgtgtccgtc gatcgacacc
     6841 gcgctccacg agacggtgta gatgcccggc tgcgtgatgt cgaccgagcc gctcaggacg
     6901 ttccggacgg cgacgtccag gtcgagcccg ccctcgccga cggtgccgcc ggccgcatcg
     6961 gtcacgacga aggcgctgtc ggggccgagc tcgcccgaga aggtgaggac gacctcgctc
     7021 ggcgccgact cgacgtccgc cccggcttcg ggcgtcgacg attcgagatc cgcgtgcgcc
     7081 atgactgccg ccgggagggc gaggagagcg acggcggcag tgacggacgc caggagcctg
     7141 ctgagacgca acgtgttcct ccgcgtgttc aggggccgcc ccaccgcgag cgcaggtacg
     7201 ccacgaggtc ctggcgctcg ccgggtgtca gctggccgat gaaggccggc atggccgtcc
     7261 cggccacgcc gttcgccata cggtagtcca gctcggcggc cgatgcgccg cggaccgccg
     7321 ccggcaggtc gcctgcgccg ggaagcgtcc ggaccggccc gtcgccaagg ccgtcgcgtc
     7381 cgtggcacgc gaggcagttc gcctggtaca gccgctcacc gcgcgccacg gactcggcgc
     7441 cggccggcgg cgaggcggac gggctggccg agacggctga cggcggcgcg ttggccgccg
     7501 cgaccaggtc gcgggagccg gcgccgaggc cagccgccag cgccaccacc acgagcgcgg
     7561 cacgcaggac cgtccgcagg ggctgtccgc gcgggtcacg gccgaggaac cacgccagga
     7621 gcagcgccgc gggcacgagc agccaggcca gcggcccggg ggggaggatc ggccacagcg
     7681 cggcgagggg tgcgggcaca ccgatcccgg agtcctccgg cggcgcctcc tccgcggggc
     7741 cggggctgct caccggcagg gcgaagctga ccgattcgtc gagcgcgccg tgccgccgga
     7801 ccaccacgtc cagtcgccac gtgccgtcca gcggcgtata ggcgccgcga gccgtgtaga
     7861 ggccgggcac ggtgcccgac ggcgtcatct cgatccgctc gctcggcaga tcggtgcccg
     7921 cgggcggcgt gaagtccagg aagaccttct gcacgtcgag ccgtggcgag ccggtctccg
     7981 cgtcgttgac cgcgacctcg tactgctggt ttcccggggc gggcaggttg acggcggcgt
     8041 gcaccgtcga cggtcccgcc gcgccgtaca ggtgcgcggg attcacctcc gcggctacca
     8101 ggcgcggcgc ccgcgcggcg gccggctgaa tcgcgaccat ggtcgcggcg gccgccacgg
     8161 caagggcggc caccacgagc tcggcgccga ccagggtggc ggtggccgcg cggcctcggc
     8221 cgcgaagggc gaagtggttc accgcgccca gacccaacgc cacgctcacg agggccgcct
     8281 tggccaggac gaggttcccg tagtcggagc cgacgacgtc ccgcggcgtg ccgagcacca
     8341 gcggggagtt ggcgacgccc gtcaggacca ccaccggccc ggcgaccagg gccacggcgc
     8401 catgccgccg caggatggac gccagtgcgg gccggaaccg ccgagctcgc gccgcgagga
     8461 cgaaggccgc cggcagggca cccagccagg ccgcgaccgc gaccaggtgg acccagtcca
     8521 gggcgccgaa caacggcccg cccgcggcgg ccgcgtgcgc cgcctggctc atggcgccga
     8581 gcgcgacgag aagcaggatc gtgcccgcga gcggcagcgc acgcccgtcg cctcctcgcc
     8641 cgcggacggc accgagcacc gcgacgacgc tcgtggccag cagggccgcc accgcgacgc
     8701 gcatggcgat cgagaatggc gtccacccga acgggccggc cacgtccagc gggatgcccg
     8761 tggacggggg agcgacgccc gagcgcgccg cggcgtcgac gatcggccgc gctgcgagcc
     8821 agaggtagag cgcggtgccc gcgacggcga ccgacgccgc gctggcgacg gtgccccacg
     8881 gcggccgtcg ctcggccagg cccaggtccg tgagcgcgcg gcggccggcc gtccaccaca
     8941 gcagcaggct gccgagggcg atgagcgatc ccagcaggcc gagccaacgg gcaagaatcg
     9001 tcgtgacgtc gacggacggt gaggtctccg acccgctgcc ggtgggtgcc gcctccgtgc
     9061 ccgtcggatc caccaggaac gcgaaaatcc ctgccgtggc gtggccatcg atgctcgaca
     9121 ccacctggta gctgacggtg tacacgccgg gctccagcgt ggggagggat gcgcggacgg
     9181 tcacgccacc gtcgaccgtc gggtcgccga cccccgccac ggcatcctgt gcctcgtcca
     9241 ggagctcgac cgacacggtg gcggggtcga cggcctccgt gaaggtcagg accacggccg
     9301 ccggtgactc ggtcagcgtc gcgttggctg ccggatcgga tgagagcagc tcggcatggg
     9361 cccatgcgcc ggccgggcga gcgaccgtgc cgatgaccac gaggagcgtc gccgccagga
     9421 gccggcggag gtgaaacgtg gtgcgatgag cgatggtcgg caacgcggac tccggagggc
     9481 accgcggcgt ggcgcggcgg cgacggggcg gtgccgagta tacgtccggc gtcgcagagc
     9541 gccgcgtcgg tcgcaggact ggcccaccgc ttgcatccat ggtgggcatc acaccaccaa
     9601 cgcgaaggag gagttcgatg gcttccgttg tcgaaagcat cgacgtcaag gttccggtcc
     9661 gcacggccta tgaccagtgg acccaattcg aggagttccc gcgcttcatg gagggcgtga
     9721 agtccgtcac gcagatgggc gacacgaacc tccactgggt cgccgaggtc ggcggcaagg
     9781 agaaggagtg ggacgccaag atcaccgagc agaagccgga tgagcgcgtc gcctggacca
     9841 acacgtccgg cgccgagaac gccggcgtcg tgaccttcca ccgcctcgac gacgagacca
     9901 ccaaggtcac gctccagatg gacgtcgacc ccgagggcat ggtcgagaac gtcgggacgg
     9961 cgctcggctt cctggatcgc cgtgtcaagg gcgacctcga gcgcttcaag gagttcatcg
    10021 agaaccgcgg cgtcgagacc ggcgcctggc gcggcgaggt cgaggccggc gcccagcggt
    10081 gaccgaccgc gagtcgaccg gtggcggcgg gttgaccggc gctaccgggg acctcacccc
    10141 ggctgacacc gatgcggcgt tcgatcccgg cgagcgccgc gaattcggcg accccgcggt
    10201 ccaggcggag atgaccgccg cccagggcgc cagggccgat gcgcagcagg gccagatcgg
    10261 ccgccccgag gaccagccgg tcggtggccc gacgaacctg gccgatcgcg acatgggcta
    10321 cggctcggag cacggtctcg acccggagga cccggcctat cgcatggagt cgcacccgcc
    10381 ggcggcccgg gcggcgaccg atgagagcga agacgcctcc gatcgctcga cccggctggg
    10441 tggtgacgag ctcctcgagg aggagccgca cttctgacgt cgggccccag cccgcggtca
    10501 ggcggcggat cgacgagcgg cgcgctttcg ggcgggccgg ttcttcgtct ccttcgttgg
    10561 ggctgacttg ccgcccgcct tcgtccgagc acccttcgcc tcggagacgc tggcgcgcag
    10621 cgcctccatc aggtcgatga ccttcggctc cggccgcctt cgcttcgcgt ttcggacctc
    10681 gccgcgcatc ttctgctcga tgagctcctc gagggcaacg cggtagtcgt cggtgtagtc
    10741 atccggcctg aatcggtcgg ccaggctctc gaccaggctg cgggccatgt ccagctcctt
    10801 ggcgcggacc ttcgcctccg caccctcggg caggtccaat gagccggtcg accgaatctc
    10861 gtccggccag aacatcgtct cgaggatcag ggtgtcgtcg taggcgcgga gggtggcgag
    10921 ccgttcacgg gtccggatgg ttacgcgcgc cagggcaacg cgctcggtct cctcgagcgc
    10981 cttcttcagg aggctgaacg ccttgccccc gacgtcggtg ggctcgagga agtagctctt
    11041 ctcgaagtac accgggtcga tctcgacggc ctcgacgaag tcaccgatgt cgatggtggt
    11101 gctgctctcc ggcttggccg cgtcgatctc ctccggatcc aggatgacgt actcgttctt
    11161 cgagacctgg aagccgcgca ccaggtcgtc ccatgcgagc tcctcgccgc cctcggtgca
    11221 gacgcgcttc tcctcgatcg gcgcctcgtg ctccttgcag aggaggcgga attggatgct
    11281 cttggactcc gtggccgcgt acagcttcac ggggatggtg accatcccga agctgatcgc
    11341 gcccttccag atcggacgca tcggtcctcc ttcgatcccc gacctagccg accgactggc
    11401 ggacgttgtc gtcctcgtcg acctcgatgc cgtacttgtc ggcggcggag agaatcttct
    11461 ttcgggcgcg ctccttggcg gccttgctct cgaactcggt ctggttgtag cgcgcgatcg
    11521 cgttgcggac gtgcgattcg tcgttgatcg gcaggtgctc cccgccgtcc gagtccacgt
    11581 acgcgaactc gtcacgatcc agcttgtcac ggtccttggc gtcgagctcg ctcatgaggg
    11641 tctcctcggc tgcgttgtac cacccactca tggcacgccg cgtgccgatt cgcggctccg
    11701 tgagcgtcgc tgtgagggtc ggaacgtccg gatggcagta ccgccattgg gcaggcgact
    11761 tctacccggc tgagttgccc acctccgagt ggctcgcgta ctacgtggag cggttcgaca
    11821 ccgtggagct caacgccagc ttctaccgcc ttccacgggc ggaggcattc gcggcgtggg
    11881 gacgccgggt gccggacggt ttccggttcg cggtgaaggc cagccgctac ctgacccacg
    11941 tcaagcgcct gaaggacccc gatgaaccgc tggaccgctt ctggacgcgc gtgcggcg
//
LOCUS       DASTYP010000003         9023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1676111, whole genome shotgun sequence.
ACCESSION   DASTYP010000003 DASTYP010000000
VERSION     DASTYP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676111"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFV59_00115"
     CDS             <1..734
                     /locus_tag="VFV59_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HEU5324269.1"
                     /translation="LELGAARVILLADDISSELEAMKAGPFAALADAHAWLVTFALSA
                     LDLPPERLVFVPTDYDGLGSPYLETLGEELPPGVEIMWTGTDVFVGTMTAEEADTVAR
                     VMRREPLVWDNYPVNDDPREHDLRIGPIRGRDADLDRHVSGVLANPALESEASLVPLL
                     TWAEYLADPSAYDADAAWRRALIRVTGNEVDAEAVAVVARALDRSALDQGWERPAPAE
                     LAAAVRSLDRLQNRRLADDLRRFLD"
     gene            1124..2377
                     /locus_tag="VFV59_00120"
     CDS             1124..2377
                     /locus_tag="VFV59_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5324270.1"
                     /translation="MNATGELFAYGFGYETGDIIAKTRVDVFNEQYSDVDVTFSESGF
                     EDQGFLTALASGDAPDVVNLPRNSIGTYIARGVLQPLTDCMDQAGLDPNVFYEGARNQ
                     VTVDGEMYALPEFFNTRVWIINNAAFEEANLDPESVDFSDWDAIAQANEALVKNDGGL
                     TRIGIDPKLPEFLAMWVRAAGGQVISDDGMESLLDTPEVAEAVDFGKSLIDANGGINE
                     FSDFRNTWDFFGENNQVAADQIGAWPMEQWYLNVLAQNSPDAEITVKPFVDRDGNTLT
                     LQDGNAWAITTDTDNPEAACAFITTMVAKDTWVAAAQARADEREADGLPNTGVYTANS
                     EADDVIFNDIVDLSDYPSLQEAVQVVLDGQETAFGFPPSPAGAEFQQALTDAVNRVLS
                     GEADAQQAMADADAEAQDAIDAAAR"
     gene            2423..3382
                     /locus_tag="VFV59_00125"
     CDS             2423..3382
                     /locus_tag="VFV59_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5324271.1"
                     /translation="MATMVQGKEVRIVGGVPWTPNTKRETLWALVFLSPWIVGLLIFT
                     AGPMLWSLYLSFTDYDPLRPDITFIGLDNYVSMLGDRRVSTSLFNTAFYTVFHVPLSM
                     GIALFLALLLNRIGGKMAGFFRTAFYLPNVTPAVAIGTLFLLLLNGSNGLVNQALGVI
                     GIDGPSWLVDPAWIKPGIILMSLWTVGNTVIIYFAAIRNVPVELYEAARIDGAGGWSQ
                     FRNITLPMISGALFFTLIINTIASLQMFSEVYTIFFGQQQGQSAGAGEAALFYVIYLF
                     RNAFEFFKMGYASALAWVLFVVILIITVIQVRGSRRFVYYEGE"
     gene            3385..4299
                     /locus_tag="VFV59_00130"
     CDS             3385..4299
                     /locus_tag="VFV59_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5324272.1"
                     /translation="MATDQHARSVDARAIDRPDVDVKEPIDWGKVAFKVGFGLVLVLF
                     TILFLYPLVWLFFASVKPGSEVFSGNLLPSVWRWDNYPRLIEQQPVLQWAWNSALVTV
                     LASVTVTISSALVAFAFAYFRFPFRGVVFGIVLASMMLPGAVTMIPNYLIWDFLGFNN
                     TLVPLWAGNLFASPFYIFMLRQFFLGLPRELFDAAKVDGANYLRMWWSVAVPLTRAAL
                     VVVFIFELKVAWTDLVRPLIFLRDIATFTLPRGLKAIIDNPSIGGEKHFELLAVGGIL
                     ITVPMIIVFFLGQRYFIEGIATTGSTGR"
     gene            4308..5015
                     /locus_tag="VFV59_00135"
     CDS             4308..5015
                     /locus_tag="VFV59_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324273.1"
                     /translation="MLVRDEAGAEPPPQPSLPVALRTGGMKLFEEIFPVLVTNLAWAM
                     LAGALVYVAGLVPLALLLLPLIAPLTTGLARVAVLAARDRVVPLRAFVDGVTDRFWLK
                     LGLGALQAVVLLVALMNVVVAPSMGGLFGAISMVLSVYVAAATFGYGLVFWTLLSDPD
                     RATMPVRRVGRLAMAVVLTRPLPVAFLLILAALAIGVMASLIVPLLFLPSFVLITVAA
                     YVLPAADLLQATARRTP"
     gene            complement(5025..6128)
                     /locus_tag="VFV59_00140"
     CDS             complement(5025..6128)
                     /locus_tag="VFV59_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEU5324274.1"
                     /translation="MTDLDDVLRDPIPLSPNRIPRFYRGGRMLAAFAGEPNAADDGRP
                     EDWVGSATRTWTPPGAGRSDLGLSAVDVGGRRTTLAQLLVEAPEALVGPTWAAVVPST
                     LGVLVKLLDAGERLPVHAHPTRNAAARLLGSAFGKTEAWIILGVRDGSVPRVWAGFRE
                     PIGHDQLGDWIARQDVEAMLGALVEHAVAPGDVILVPGGQPHAIGAGVFLLELQEPTD
                     FSVVAETRGFPIDPADASLRLGWEAAIEFFATEASGQLRQRPSEVAPTVTNLFGPAAD
                     AFFRALRHAPAAGQTTEPPFEPGFAVGVVTGGRGVVRGARREVPLERGVTFALPAAAS
                     SSTISAASGGLELIWCLPPSQPPAQAHVEGLPA"
     gene            6262..7269
                     /locus_tag="VFV59_00145"
     CDS             6262..7269
                     /locus_tag="VFV59_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5324275.1"
                     /translation="MTPAPMLGIDIGGTKLAVGVATPDGSLLAERVEPSRAHEGPDAM
                     ISRAVDLARAATRDAGVPVAEIGAVGIACGGPLDPWRGVVRNALNNPGWIDIPIVRLV
                     SDALGRPTYLDNDANAAALGEHRFGAGRGVDHLVYLTISTGVGGGVILDGRLYRGANG
                     NAAELGHISIDHRGRPCHCGSIGCIEACCSGTNIAARTREALAATEEASRLRAVPLDR
                     LTAADVTAAVRDGDPLAERIWGETTTMLGAGIVSIIHAFNPQLVILGGGVTRAGDLLF
                     DPVRRVVEERAMPWLREAVRVVPAELGERSGILGAVAVALDGPDLAATAPSSDTERTH
                     A"
     gene            7262..7855
                     /locus_tag="VFV59_00150"
     CDS             7262..7855
                     /locus_tag="VFV59_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5324276.1"
                     /translation="MPDVDTAALEREWQEHVRVVTDAAALVPRAAELADRLCAALERG
                     GKLITFGNGGSAADAQHFAGELLGRFRATRRPLPAMALSTDPSVVTCIANDFSYEDLF
                     ARQVTALATADDVAIGITTSGRSENVVRGLRAAREAGALAVAWTGADPGPAGEAADLV
                     LAAPSTTTARIQEVHTLLMHTICVAIDAWDAAREPRP"
     gene            7852..>9023
                     /locus_tag="VFV59_00155"
     CDS             7852..>9023
                     /locus_tag="VFV59_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU5324277.1"
                     /translation="MTVGEPTEARRVVDPRTATLHMIGNAHIDAVWLWPWEEGFAEVK
                     ATFQSALDRMEEYPEFVFTCSSAAYYAWVEANAPSMFEQIRRRVAEGRWRITGGWWVE
                     PDCNIPSGESFVRQALLGQHYFASRFGRIATVGYNPDAFGHAGSLPQLLRKARLTGYT
                     FLRPEPWEKELPGNLFWWESADGSRVLTFHINFGYTVWGDLEEHVRKHLDRFVDGTDA
                     LLCFYGVGNHGGGPTKANIETIRSLNQLDELPALRHSWPSAFFNAVDVPDDLPVVRDE
                     LQHHAVGCYAAVSGIKRRNRQAEELLGTAERWASIAAREGLQPYPDDLSGAWRNVLFN
                     QYHDILAGTSIEPAYDDARSQLGESMAVADRALTNGVQAISWAVDTRGPAGARPIVV"
BASE COUNT         1289 a         3185 c         3132 g         1417 t
ORIGIN      
        1 acctcgagct gggggccgca cgcgtcatcc tgctggccga cgacatctcc agcgagctcg
       61 aggccatgaa ggccggaccg ttcgccgccc tggccgatgc gcacgcctgg ctggtgacct
      121 tcgccctgtc cgcgctcgac cttccgcccg agcgcctggt cttcgttccc acggactacg
      181 acggccttgg ctcgccgtac ctggaaacgc tcggcgagga gctgccgccg ggggtcgaga
      241 tcatgtggac cgggacggac gtcttcgtcg gcaccatgac cgccgaggag gccgacaccg
      301 tcgcgcgcgt catgcggcgc gagccgctgg tatgggacaa ctacccggtc aacgacgacc
      361 cgcgcgagca tgatttgcgc atcgggccga tccgtgggcg cgacgccgac ctggaccgcc
      421 acgtgagcgg cgtgctcgcc aacccggcgc tcgagtcgga ggccagcctg gtcccgctcc
      481 tcacttgggc cgaatacctg gccgatccgt ccgcctacga cgccgatgcc gcctggcgcc
      541 gagcgctcat ccgggtgact gggaacgagg tggacgcgga ggcggtcgcc gtcgtcgcgc
      601 gcgcgctcga tcgatcggcg ctggaccagg gatgggagcg cccggctccg gcggagctgg
      661 ccgcggccgt ccgttcgctg gaccggctgc agaaccggcg gctcgcggac gacctgcgtc
      721 gcttcctgga ctaggcagca cgcatctctg ccggcgtcag cacctggccg cgtcgctacg
      781 gtctcggacg attctggcag ccgttgtgca gtcatgggtc aggccaccgg gcgccgtgcg
      841 agacgggcgc gcttctcggc ggcgagacgc taggcttcgg attccgcaac caaccgagtc
      901 agcgtcgccc atccaggggg accgattgtt gcgcatggga ggtaggcacc gaatgaccag
      961 atggtcacga ctcgtcccac tcctcgccat cgtcatgctc gtcttggcag cctgtggccc
     1021 ctccggggag tcctccagtg ctgccgcgtc cgagcctggc gggagccagc cagccgggtc
     1081 cgagccggcg gcgagcggga gtggcggcga gggaaccggc ggcatgaacg ccaccggcga
     1141 gctcttcgcg tacggttttg gctacgagac cggcgacatc atcgccaaga cgcgcgtcga
     1201 cgtcttcaac gagcagtact ccgacgtcga tgtcacgttc agtgagagcg gcttcgagga
     1261 ccagggcttc ctgacggcac tcgccagcgg cgacgcgccg gacgtggtca acctgccgcg
     1321 caacagcatc ggcacgtaca tcgcccgcgg cgtcctgcag ccgctgaccg actgcatgga
     1381 ccaggcgggc ctcgatccga acgtcttcta cgagggcgct cggaaccagg tcaccgtcga
     1441 cggcgagatg tacgcgctgc cggagttctt caacacgcgg gtctggatca tcaacaacgc
     1501 cgccttcgag gaggcgaacc tcgatccgga gagcgtcgac ttcagtgact gggatgcgat
     1561 cgcccaggcc aacgaggccc tggtcaagaa cgacggcggg ctgacccgca tcggcatcga
     1621 tcccaagctg cccgagttcc tggccatgtg ggtccgcgcg gccggcggcc aggtcatcag
     1681 cgacgacggg atggagtccc tgctcgacac gccggaggtt gccgaggccg tcgacttcgg
     1741 caagagcctg atcgacgcca acggtggcat caacgagttc agcgacttcc gcaacacctg
     1801 ggacttcttc ggtgagaaca accaggtcgc ggccgaccag atcggcgcgt ggccgatgga
     1861 gcagtggtac ctgaacgtcc tggcgcagaa ctcgccggat gcggagatca ccgtcaagcc
     1921 gttcgtcgat cgcgacggca acacgctgac cctgcaggac ggcaacgcct gggcgatcac
     1981 gaccgacacc gacaaccccg aggcagcatg cgccttcatc accaccatgg tggcgaagga
     2041 cacgtgggtt gccgccgctc aggcccgcgc cgacgagcgc gaggctgacg gtctgccgaa
     2101 caccggcgtc tacacggcga acagcgaggc ggacgacgtg atcttcaacg acatcgtcga
     2161 cctgtcggac tacccgtcgc tgcaggaggc ggtgcaggtg gtgctggacg gccaggagac
     2221 cgcgttcggc ttcccgccga gcccggccgg tgccgagttc cagcaggcac tgaccgatgc
     2281 ggtcaaccgc gtgctgtcgg gtgaggcaga tgcgcagcag gcgatggccg acgccgacgc
     2341 cgaggcgcag gacgcaatcg acgcggcggc ccgctagggg tcgctcgcgt cggaccggag
     2401 ggtagagcgg gagggttctc gaatggccac gatggtccag ggcaaggagg tccgaatcgt
     2461 gggcggcgtc ccgtggacgc cgaacacgaa acgcgagacg ctgtgggcgc tcgtcttcct
     2521 ctcgccctgg atcgtcgggc tcctcatctt cacggccggc ccgatgctgt ggagcctgta
     2581 cctctccttc accgactacg acccgctgcg cccggacatc acgttcatcg ggctcgacaa
     2641 ctacgtctcg atgctgggcg accggcgggt ctcgacttca ctcttcaata cggccttcta
     2701 caccgtcttc cacgtgccgc tgtcgatggg gatcgcgctc ttcctggcgc tgctcctcaa
     2761 ccggatcggc gggaagatgg ccggcttctt ccgcaccgcc ttctacctgc cgaacgtgac
     2821 cccggcggtc gcgatcggca cgctgttcct gctgctcctc aacggctcga acggcctcgt
     2881 gaaccaggcg ctgggcgtca tcgggatcga cgggccgagc tggctggtcg acccggcgtg
     2941 gatcaagccg ggcatcatcc tgatgagcct ctggacggtg gggaacaccg tcatcatcta
     3001 cttcgcggcc atccgcaacg tgcccgtcga gctctacgag gcggcacgca tcgacggggc
     3061 cggtggctgg agccagttcc gcaacatcac gctgccgatg atctcggggg cgctcttctt
     3121 caccctgatc atcaacacca tcgcctccct gcagatgttc agcgaggtct acacgatctt
     3181 cttcggacag cagcagggac agtcggcggg agccggtgag gcggccctct tctacgtgat
     3241 ctacctgttc cggaacgcgt tcgagttctt caagatgggc tatgcctcgg cgctggcgtg
     3301 ggtgctcttc gtggtgatcc tgatcatcac ggtcatccag gtgcggggca gccgccgctt
     3361 cgtgtactac gagggcgagt gacgatggct actgatcagc acgctcgctc ggtcgacgct
     3421 cgcgccatcg accgtcccga cgtcgacgtc aaggagccca tcgactgggg caaggtggcc
     3481 ttcaaggtcg gcttcggcct cgtcctcgtc ctcttcacga tcctgttcct ctacccgctg
     3541 gtgtggctgt tcttcgcctc ggtgaagccg gggtccgagg tcttcagcgg gaacctgctg
     3601 ccctccgtct ggcggtggga caactacccg cggctgatcg agcagcagcc ggtcctccag
     3661 tgggcctgga acagcgcgct cgtgacggtc ctggcgtccg tcaccgtgac gatcagctcg
     3721 gcactggtcg ccttcgcgtt cgcctacttc cgcttcccgt tccgcggggt cgtgttcggc
     3781 atcgtcctcg ccagcatgat gctgcccggc gccgtgacca tgatcccgaa ctacctgatc
     3841 tgggacttcc tgggcttcaa caacacgctg gtgccgctgt gggcgggcaa cctgttcgcg
     3901 tcgccgttct acatcttcat gctgcgccag ttcttcctgg gcctgccgcg cgagctgttc
     3961 gacgcggcca aggtggacgg ggcgaactac ctgcgcatgt ggtggtcggt ggccgtgcca
     4021 ctgacccgcg ccgcgctggt ggtggtcttc atcttcgagc tgaaggtcgc ctggaccgat
     4081 ctggttcgac cgctgatctt cctgcgcgac atcgccacct tcacgctgcc gcgtggcctg
     4141 aaggcgatca tcgacaaccc ctcgatcggc ggtgagaagc acttcgagct gctggccgtc
     4201 ggcggcatcc tgatcaccgt gccgatgatc atcgtcttct tcctgggcca gcggtacttc
     4261 atcgagggca tcgccacgac cggctcgacc ggccgctgac gatccacgtg ctcgttcgtg
     4321 acgaagcggg ggccgagccg cccccgcagc cgtcgctgcc cgtcgccctc cgcaccggcg
     4381 gcatgaagct gttcgaggag atctttccgg tcctggtcac gaacctggcg tgggccatgc
     4441 tggccggggc cctggtctac gtcgccgggc tcgtgccgct cgcgctcctg ctgctgccgc
     4501 tgatcgcgcc gctgaccacc gggttggcga gggtcgccgt gctggccgcg cgcgaccgcg
     4561 tggttccgct gcgcgccttc gtcgacggcg tgaccgaccg cttctggctg aagctcgggc
     4621 tcggcgccct gcaggcggtg gtgctgctcg tcgcgctcat gaacgtcgtc gtcgccccgt
     4681 cgatgggggg gctgttcggg gcgatctcga tggtcctgtc ggtctatgtc gcggccgcga
     4741 ccttcgggta cgggctcgtg ttctggacgc tgctgtcgga tccggaccgc gcgaccatgc
     4801 cggtccgccg cgtcggtcgg ctcgcgatgg ccgtggtgct gacccggccg ttgccggtcg
     4861 ccttcctgct gattctcgcg gccctcgcca tcggcgtcat ggcgtcgctg atcgtgccgc
     4921 tgctgttcct gccgtcgttc gtgctgatca ccgttgccgc gtacgtgctg ccggcggccg
     4981 acctgctcca ggcgaccgcg cgtcggacgc cctgacgctt cgcctcaggc cggcagcccc
     5041 tcgacgtggg cctgcgccgg cggctgggag ggcggcaggc accagatgag ctcgagaccg
     5101 ccggatgcgg ccgagatcgt cgagctggag gcggccgccg gcagggcgaa cgtgacgccg
     5161 cgctcgagag gcacctcgcg ccgcgcgccg cggacgactc ctcggccgcc agtgaccacg
     5221 ccgaccgcga agccgggctc gaacggcggc tcggtcgtct ggccggccgc gggagcgtgg
     5281 cgaagcgctc ggaagaaggc atccgcggcc gggccgaaca ggttcgtgac ggtgggcgcg
     5341 acctccgacg gccgctggcg cagctggccg gacgcttcgg tggcgaagaa ctcgatggcc
     5401 gcctcccacc ccaggcgcag ggacgcatcg gcggggtcga tcgggaagcc gcgcgtctcg
     5461 gccacgaccg agaagtcggt cggctcctgg agctcgagca ggaagacgcc ggcgccgatg
     5521 gcgtgcggct gcccgccggg gaccaggatc acgtcccccg gcgcgaccgc gtgctccacc
     5581 aacgccccga gcatcgcctc cacgtcctgc cgtgcgatcc agtcacccag ctggtcatgg
     5641 ccgatcggct cccgaaagcc ggcccacacg cgcggcaccg atccgtcgcg cacgcccagg
     5701 atgatccacg cctcggtctt gccgaacgcc gagcccagca gccgggcggc cgcgttccgc
     5761 gtcgggtgcg cgtgcaccgg cagccgctcg ccggcgtcga gcagcttgac cagcacaccg
     5821 agcgtcgacg ggacgacggc agcccacgtc ggcccgacca gcgcctcggg tgcctcgacc
     5881 aggagctgcg ccagcgtcgt gcggcgaccg cccacgtcga cggcgctgag gccgaggtcg
     5941 gagcgtccgg cgcccggggg cgtccaggtc cgcgtcgcgg atccgaccca gtcctcgggt
     6001 cgaccgtcat cggcggcgtt cggctcgcca gcgaacgcgg ccagcatgcg cccgccacgg
     6061 tagaagcgcg ggatgcggtt gggggacagg ggaatggggt cgcggagcac gtcgtccaga
     6121 tcggtcatca ctggctctcg acggtagcag cgggcgcaac ttctccgcga ccagcctcta
     6181 cgataggtcg acgatcgtgc tcgccctcga ccccctcgct ccccgtaccg cgcgcctcgt
     6241 ccccgccgag gtcgttcgcc gatgaccccg gcgcccatgc tcggcatcga catcggcggg
     6301 acgaagctcg ccgtcggcgt ggcgacgccc gacgggtcgc tcctcgccga acgggtcgag
     6361 cccagccgcg cgcacgaggg gcccgacgcg atgatctcgc gcgccgtgga cctggcgcgc
     6421 gccgcgaccc gcgacgccgg cgtgccggtg gccgagatcg gggcggtcgg catcgcctgc
     6481 ggtgggccgc tggatccgtg gaggggcgtc gtccgcaacg cgctcaacaa cccgggctgg
     6541 atcgacatcc cgattgtccg gctggtgagc gatgccctcg gtcgcccgac gtacctcgac
     6601 aacgacgcca atgccgccgc gctcggcgag catcggttcg gtgccggtcg cggcgtcgac
     6661 cacctcgtct acctcaccat ctcgaccggc gtcgggggcg gagtgatcct cgatggccgc
     6721 ctctaccgcg gggccaacgg gaacgccgcg gagctggggc acatctcgat cgaccatcgc
     6781 gggcggccgt gccactgcgg gtccatcggc tgcatcgagg cctgctgctc ggggacgaac
     6841 atcgccgctc ggacgcgtga ggcactggcg gcgacggagg aggccagccg cctgcgggcc
     6901 gtcccgctcg accggctcac cgcggccgat gtgacggcgg cagtgcggga cggtgacccg
     6961 ctcgccgaac gcatctgggg cgagacgacc acgatgctcg gcgcggggat cgtcagcatc
     7021 atccacgcct tcaacccgca gctcgtcatc ctgggcggcg gcgtcacgcg tgccggcgac
     7081 ctgctgttcg acccggtccg gcgcgtggtc gaggagcgcg ccatgccgtg gctgcgcgag
     7141 gccgtccggg tggtcccggc ggaactgggc gagcgcagcg ggatcctggg cgccgtcgcc
     7201 gttgccctgg atggcccgga ccttgcggcc accgcaccat cgtccgatac agagaggacc
     7261 catgcctgac gtcgacacgg ccgcgctcga gcgggaatgg caggagcacg ttcgcgtggt
     7321 gaccgatgcg gcggcactcg tgccgcgcgc cgcggagctg gccgaccggc tgtgcgccgc
     7381 gctcgagcga ggcggcaagc tcatcacgtt cggcaacggg ggcagcgccg ccgacgccca
     7441 gcacttcgcc ggcgagctgc tgggccggtt ccgggcgacg cgccggccac tgccggcgat
     7501 ggccctgtcc acggacccgt cggtggtgac ctgcatcgcc aacgacttca gctacgagga
     7561 cctgttcgcg cggcaggtga ccgcgctggc cacggcggac gacgtcgcga tcggcatcac
     7621 caccagcggt cgcagcgaga acgtggtgcg tggcctgcgc gccgcgcggg aggccggggc
     7681 gctggcggtg gcctggaccg gcgccgatcc gggacccgcc ggcgaggcgg ccgatctggt
     7741 cctggccgct ccctcgacca ccaccgcgcg catccaggag gtgcacacgc tgctcatgca
     7801 caccatctgc gtcgccatcg acgcctggga cgctgcccgg gagcctcgcc cgtgacggtg
     7861 ggggagccga ccgaagcgcg gcgggtcgtg gacccgcgaa ccgcgaccct gcacatgatc
     7921 ggcaacgcgc acatcgatgc ggtgtggctg tggccgtggg aggagggctt cgccgaggtc
     7981 aaggcgacgt tccagtcggc gctcgaccgg atggaggagt acccggagtt cgtcttcacc
     8041 tgctcgtccg ccgcgtacta cgcgtgggtc gaggccaacg cgccatcgat gttcgagcag
     8101 attcgccggc gcgtggccga gggccgctgg cggatcaccg gcggctggtg ggtcgagccc
     8161 gactgcaaca tcccctccgg ggagtcgttc gtgcgccagg cgctgctcgg gcagcactac
     8221 ttcgcgtcgc gcttcggccg gatcgccacc gtgggctaca acccggacgc cttcggtcat
     8281 gccggttcgc tgccgcagct gctgcgcaag gcgcgcctca ccggctacac cttcctgcgg
     8341 ccggagccgt gggagaagga gctgccgggc aacctgttct ggtgggagtc ggccgacggc
     8401 tcgcgcgtcc tgaccttcca catcaacttc ggctacaccg tctggggcga cctggaggag
     8461 cacgtccgca agcacctcga ccgcttcgtg gacgggaccg atgcgctgct ctgcttctac
     8521 ggtgtcggca accacggcgg cggtccgacc aaggcgaaca tcgagaccat tcggtcgctg
     8581 aaccagctcg acgagctgcc ggcgctgcgc cactcgtggc cgtccgcgtt cttcaacgcg
     8641 gtcgacgtcc cggacgacct gccggtggtg cgcgacgagc tccagcatca cgcggtcggc
     8701 tgctacgcgg cggtgtccgg catcaagcgc cggaaccgac aggcggagga gctgctggga
     8761 accgcggagc gatgggcgtc cattgccgcc cgggagggac tccagcccta ccccgacgat
     8821 ctgtcgggag cgtggcgcaa cgtcctgttc aaccagtacc acgacatcct tgccggcacg
     8881 agcatcgagc cggcctacga cgacgcgcgc tcgcagctcg gcgagtccat ggccgtcgcc
     8941 gaccgggcac tcaccaacgg cgtgcaggcg atcagctggg cggtcgacac gcgcggaccc
     9001 gcgggggcac gcccgatcgt cgt
//
LOCUS       DASTYP010000004        22052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_219009, whole genome shotgun sequence.
ACCESSION   DASTYP010000004 DASTYP010000000
VERSION     DASTYP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 22052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22052
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219009"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VFV59_00160"
     CDS             <1..1300
                     /locus_tag="VFV59_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5324278.1"
                     /translation="NGQRQIGINVVLNQDISDAVVRDISRFGRVNDTFPQIDALTMKV
                     READLAAVQALPYVAAAAPDAKRIGKPVPTAPMDDFAAGISTWDHDAINVTDFGASRT
                     IGYDGEGVYVAVLDTGLLSSWRYYFGEERIADEFAIAFGGGGGERGSVSTQPNKWQTD
                     QDSHGTHVTSTILGYNLRDTAVNGVAPMATVIPVKVLNNNGSGWSSMVAAGIVYVADL
                     KASGALGDAPVVINMSLGGPVQEPVEKAAVDYAISKGVILVASAGNEGEAGMGYPGAY
                     APVISAAASGWVGEWAPGAGSWWQRDVPDPTDVDDFYITDFSSRAINAQQDLDVAAPG
                     SWIVGPYQVNGQLSYYYLGGTSMASPHVAGTVALMAQKDPSLTATQAESILEGSAIAL
                     AAGCRDVIDPNVGDYVEVCWGSDATGHGLMDSAAAVAATP"
     gene            complement(1353..2486)
                     /locus_tag="VFV59_00165"
     CDS             complement(1353..2486)
                     /locus_tag="VFV59_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324279.1"
                     /translation="MGGAPPPPELGVRSLTGITDLPARLAALNGPARERADRLLDASL
                     VHGRTVPPPELLSWLQRTFGSVDAVRKQTIVKVTNLATLEAALFAPLRAHRPIDGPAH
                     RDDLAAQLATTGEDPFCDPETGTPGNIFGRVTGPRITTGANAALADAHHGVLVFHAHD
                     PLAFDAELVAEVISTGRAWADLARAEDPSAANYLLIWNCLWRAGGSIVHGHAQALLGG
                     GPEYARLARFQRDAIAYRASFDASLVDDLVALHRDLELAADRAGGVSVLAHLTPIKER
                     EVLVIGRPGMDERDPAFADAVASTLVAYRDRMGVRSFNLALWRPPVGATPDGPWRDIP
                     PIVRMVDRGDPLQRASDIGAMELYGTPIVGSDPYEVIAALSQA"
     gene            complement(2441..3031)
                     /locus_tag="VFV59_00170"
     CDS             complement(2441..3031)
                     /locus_tag="VFV59_00170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5324280.1"
                     /translation="MSSLPERRPIIRGEQVYLRPSERTDLPTFVRWFNDAEVTHNLAM
                     RAPMSEPSETEWYEGMLARQGKTDYHFVICLLADDRPIGTIGLHGIDWRSGTAEFGIA
                     IGEKSEWNHGYGTDALRAICDFGFGELRLERISLHVYDGNDRARRSYEKAGFRHEATL
                     RHAHFARGEHLDVHVMGLLRDEWAALPHRRSWEYGA"
     gene            3065..3517
                     /locus_tag="VFV59_00175"
     CDS             3065..3517
                     /locus_tag="VFV59_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEU5324281.1"
                     /translation="MAATRTATVTWTGTLAQGSGTVSAGTSELFTDLPVSWASRTEEA
                     NGQTSPEELLAAAHASCFSMALSGALGRAGTPPEHLHVSATVTFDKVGEGWGVTTSEI
                     DVVGVVPGLDEAAFHEAARGAGDNCPISKALAGNVRITVTAALEDPHD"
     gene            3615..4124
                     /locus_tag="VFV59_00180"
     CDS             3615..4124
                     /locus_tag="VFV59_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324282.1"
                     /translation="MTRPSTIRGAALATGALLVAAGVAFAMPGPDRTDAEDDVAAASA
                     SPVATFGAELDSPSPSPTASAADDDGLEDEDAAEASSSPELEELFEDESESPSASPED
                     EDGEDVDEDDASASDEAEASPDDGDDDEAAGDDHAEASPDDGQDHDSGADGEDDDSGS
                     GSDDSAHDD"
     gene            4176..4718
                     /locus_tag="VFV59_00185"
     CDS             4176..4718
                     /locus_tag="VFV59_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5324283.1"
                     /translation="MDDALIAALRAGDRDAFAALVERETGPIYRTCLRILGVGADAED
                     VAQETFVIAYRSLRGYRGAGSLRGWLMRIASRQAFRRLQQRRPSDPLEGVAEERIADR
                     GADPARLTVARERHGTVRGSVASLPEPYREVVVLRFFGELSLLEIAEATGRPLNTVKT
                     HLRRGLQRLRPLLGDEEVVS"
     gene            4715..5272
                     /locus_tag="VFV59_00190"
     CDS             4715..5272
                     /locus_tag="VFV59_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324284.1"
                     /translation="MTRFPFGSEELGRTDPELDRIGAQLEGYADETRSTPPEGLASRI
                     LAAVDAEPEPRVGAWARFSAALVGLRRPAQALAAVAVLGAAVVGALALAEIADRTRTD
                     GFGSSSSPLASPAVTPSPSATPSPTLAPTPSPTPTASPTPTLSPTPEAVQTPDASADQ
                     ETPEPSASDDDGGDDHSGSGGGGDD"
     gene            complement(5280..5651)
                     /locus_tag="VFV59_00195"
     CDS             complement(5280..5651)
                     /locus_tag="VFV59_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5324285.1"
                     /translation="MEHGDFTHIELPADEPERAKRFYAELFDWRFSDAPGFDGYHLFT
                     TPSGEQGVGGAIGKRGEMAPESMRTYINVRSVDDVLARVPELGGRVIAGKQEVPGMGW
                     FGVFADSEGNELAVWEALPRS"
     gene            complement(5908..6336)
                     /locus_tag="VFV59_00200"
     CDS             complement(5908..6336)
                     /locus_tag="VFV59_00200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5324286.1"
                     /translation="MTDAPLPIDPWRFTSRYSVRQHELDLLGHVNNAVYLHWIEQVAI
                     DHVEAMGVGRAWSLDRGGAWVVREHHVTYRQPVVYGDIVLVTTLPQEISGVRGLRRTE
                     IRRESDGALTTEALTHWVWVRLSDGRPTRVPPDILERYRR"
     gene            6488..7879
                     /locus_tag="VFV59_00205"
     CDS             6488..7879
                     /locus_tag="VFV59_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5324287.1"
                     /translation="MDAAVTLHLAESPTDLDAFARSLRGTVVRPDDEAYDTDRLVWNV
                     AHQGNPSVIVRAADAADVAAAISFACRNDLEIAVRSGGHSLAGHSSGDGTLVIDVRDM
                     KGIHIDPDQGTVWAGAGLTAGELTNALAAHGLAVPFGDTGSVGLGGITLGGGIGWLVR
                     KHGLTVDSLVAVEMVTASGEVVTASETENADLFWALRGGGGNFGVVTRFRYRTHRLDG
                     ILGGAIFLPPTREVFRSLVPIAGSAPEELTTIGFVMPIPPLPFVPEEHHGKPALALMF
                     VYAGDPEAGRAAIAPFTRVATPYAVAAMPMPYPGIYEFTAEGSVAHPSRTRSVFMETF
                     DEAAVETLVSAIETAPPGSMVQFRVLGGAMARVAGDATAFAHRSAAVQVVIVNAFPEP
                     GMEEAGVAWNESLFRALEPRASGVYVNFLEDEGDARIRDAYPNGTYERLAAVKRRYDR
                     YNIFHRNQNVRPA"
     gene            complement(7950..8025)
                     /locus_tag="VFV59_00210"
     tRNA            complement(7950..8025)
                     /locus_tag="VFV59_00210"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7990..7992),aa:Gly,seq:ccc)
     gene            complement(8151..8381)
                     /locus_tag="VFV59_00215"
     CDS             complement(8151..8381)
                     /locus_tag="VFV59_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324288.1"
                     /translation="MATKAYDERLAHAARIRQVARDRHDEQRPFVPQAHRHITVARVA
                     AAATAVALTVAGAATAVAAHPTLGGGAHILVR"
     gene            8686..9381
                     /locus_tag="VFV59_00220"
     CDS             8686..9381
                     /locus_tag="VFV59_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5324289.1"
                     /translation="MHDPSEVVEGLRRCALFAHVDEEGLRSLAGRMRRRRFKRNEVIF
                     HQGDIGDSLQVVGAGGVKIVLPSPEGEEAIIASLKPGDFFGELALLDGAPRSTTATAI
                     EPTETLTLPREEFLRLLSEDPRLVRALLHALAEELRRLTGHVEELHFLDLSGRLAMRL
                     VRLARETEPNATADVRLDWPFTQSDLAAMVGGTRQSVNRLLSGLVDDGVIEIERDTLV
                     IRDLDELEARTVR"
     gene            9615..9953
                     /locus_tag="VFV59_00225"
     CDS             9615..9953
                     /locus_tag="VFV59_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324290.1"
                     /translation="MSDDRNLDTEGHIRRRTDDESDVEGHGFSLGRATEDDDTEGHGF
                     SLGRATEDDDTEGHGYSLGRATEDEPDTEGHGYSLGRATEDDDTEGHGFSLGRATEDD
                     DDTEGHIFVR"
     gene            9957..10736
                     /locus_tag="VFV59_00230"
     CDS             9957..10736
                     /locus_tag="VFV59_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5324291.1"
                     /translation="MADQPPAAALAALVAAARRSAIGRRVQGDVETRLLQSIVDATAR
                     LFDAEASSIALFERDPDRLEFRVAAGAQGAGAIGLTVPPTKGIVGFVFSTGQAIALSD
                     VTSDPRFDKTAAQRTGYVPRSIAAVPLLDEGDPVGVLQVLDKRGSPTFSLQDMELLGV
                     FAGQATVAINAARVQRDTTRLMRAVLTAIGPDLAPDDIEAVVSSVAAELDVDEEAPFW
                     RVVDQVARLRSLSDREAALVADILEVLSAHAARAQRGRGSR"
     gene            10774..11625
                     /locus_tag="VFV59_00235"
     CDS             10774..11625
                     /locus_tag="VFV59_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5324292.1"
                     /translation="MSEPLLPAWSEPFADRQRGLRRRARLRGVTRDWAWGGATGAGIR
                     VGIIDSGLENDHPRLRGRVVQNVAVEMGPDGPVVVDDEPRDLFGHGTACGSIVLDFAP
                     EAELVSIRVLGADLRGKGTAFAAGLEWAIEQGLHVCNLSLSSKSESLYPVFHDLVDRA
                     YFASIALVSAANNVPAPSYPSLFASVFSVAAHGEPDPWRIYYNPSPPVEFGAWGVDVP
                     IAWKDGGSTVATGNSFAAPHVAGIVARIRSKHPELTPFEIKAILASVADNPGRRHPWR
                     HRTPSAG"
     gene            11622..13244
                     /locus_tag="VFV59_00240"
     CDS             11622..13244
                     /locus_tag="VFV59_00240"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324293.1"
                     /translation="MKLARTYLDLFRRNPSLARLLAGEFISGIGDWLYLVAILVVVYA
                     ESESPVLLGIVGAARILPYVLLSVPAGMAADRFDRRMVLLVTDIARGILMVVLVVLVI
                     VGAPTLAIIAVSVLAACFSTFFGPAIASLLPMLVDERDLGPANSAWATLDNIAFIVGP
                     AVAGVLLASGGLEFAFALNAVSFAVVAIVLWRLEVPQRKVPTATAPSDEAPPPQQGFR
                     DLLRPLAGPFVLDAATSIVGGGISVMTVVIAVDVLGAGEAGTGYLNAATGVGGVVAGV
                     AGGALLARRLHVPLLLGGIVGGAGLAALALTGDLALAMVAIGVAVAGLLLLEIVNTTL
                     IQRIVPDQLRGRAMGILQTSSAVLYAGGSLVMPLLADAIGVTPVLVGAGALVLLGVVG
                     ALLLSREAEEAAPIGVERSRLLEHPIFAGLPTPRLEAAARDLGEVPMAAGDVVVRQGD
                     AADRFYLIAEGTLRVTQTRDGKQVELRTLGPGDVFGEVGLLNRSPRTATVTALTDGRL
                     LALDGEDFLELVSSGPGLSSRLLDLYRGAISR"
     gene            13298..14632
                     /locus_tag="VFV59_00245"
     CDS             13298..14632
                     /locus_tag="VFV59_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5324294.1"
                     /translation="MGHLRSRRLVPLLLLALAGCASGTAQPTPVSFEADPSAIRNRLA
                     GLQEIADANDGVRTVGTVGYEASVDRLAADLVELGYAVDTPQVDFTGFSDEGSRVEVG
                     NRTFWSPDEVKALIYAAAGDVTGPVAILDGSGCDPGDFAGVPEGAIVVTTGSGCYRRQ
                     QAMNAADAGAVAFVVGYPDRGAAEIYRPTLIDPGGITLPVVSATGDAVQALRRAGGET
                     ARVVTDTRRDPSTFRNVVATLGSGSSVLVIGAHLDSVLDGPGINDNGSGVAAVLEVAR
                     GLQQHGVPDGWRVVIGLWGAEEFGDIGSRAYADAAGDGVEAYLNLDMAGSLNGATLVY
                     DDADAAPGSERLTELYGTWLTAQGEPYQPVDLSGSSDHASFTALGIPTGGLFSGASET
                     GPASRPGASASGPVPDPCYHLACDDLDNVDIERVVLFADATYAVALQLMTGE"
     gene            complement(14680..15606)
                     /gene="rsgA"
                     /locus_tag="VFV59_00250"
     CDS             complement(14680..15606)
                     /gene="rsgA"
                     /locus_tag="VFV59_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU5324295.1"
                     /translation="MTETASGLVLRSRSGFVTVLTDDGERVEARLRGRLKKERQSADI
                     VVIGDRVRVEHLPDGTGAIASVEPREAVFSRRQPGPRGSWREDVLVANPDLVAAVFAC
                     AHPPPNPRLIDRFLVVAEYNEVPTLIVANKVDLVGRGAAEEIFGAYQRIGYDVVYTSA
                     KARIGLDELHQRLAGRLSIVTGPSGVGKSTLLNAMQPGLRLATGEVSEVLHKGRHTTT
                     HAELLPIDDAAGGYVADTPGIRELGLWEIPSTELAWCFPEFRAHLGGCAFNDCTHLHE
                     PRCSLRAAVGAGEVSEDRYDSYRRMLTGDLAS"
     gene            complement(15614..16483)
                     /locus_tag="VFV59_00255"
     CDS             complement(15614..16483)
                     /locus_tag="VFV59_00255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5324296.1"
                     /translation="MPATGMKLRDATPDDLPALAALRESVGWGVQDWALRAVLESTRG
                     IYVVAEDADGAVIASGSGMVYGRLGVVGNMIVAPEHRRRGLGAQILEAILGHLSAAGC
                     VRQELYATTDGRPLYQRYGFALVEPGSHVRLPRAAFEADATDDGPNAIRRGGPADLAR
                     LAAYDEPRFGADRSELLVRMLADPERPVAIAERDEGIAGYAWLRPDGERVGPLLADDP
                     TIAQGLVRDAFARAPQATELAINLPTSNRPGMAWLSQLGIGVDPWDGRMARGEELPRR
                     QDTVYASVVGALG"
     gene            16518..17681
                     /locus_tag="VFV59_00260"
     CDS             16518..17681
                     /locus_tag="VFV59_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5324297.1"
                     /translation="MEREIDVLLVGAGVASARCARTLRRNGFDGSILLVGDEPMPPYN
                     RPPLSKELLREDLPPELLMAEPASFYERRAVEVRTGVTVTSLDAETRRATLEDGSTLR
                     YGRCLLATGAEPLRLPVPGGDLGLPLRTVADAQRLRARAQDADPGTTVAIVGGGLIGV
                     EVASGLAALGLRPTILERAGALWAGSLGHELAAWAAARLADAGVVTRTDASVTGIEPG
                     AVLVGEERMEARFSVVGIGVRPRVAMAAAAGLVVDDGIVVDAEQRTSDERIWAAGDVA
                     RTAGRRVEHWHAAREAGERAALSMLGRPVPPLPVPWTFTEVAGTPVDILGTPDDWDEE
                     RWIGGGRVLAYRSGAHVVQLAVIDAALDPGRARALVAAPASVAEVEREFTANS"
     gene            17821..19233
                     /gene="sufB"
                     /locus_tag="VFV59_00265"
     CDS             17821..19233
                     /gene="sufB"
                     /locus_tag="VFV59_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5324298.1"
                     /translation="MALPNPALSQIPTGYKYGWNDPEAKSINIARKGLSADVVEEISR
                     FKGEPEWMMKLRLKAYRHFEARPMPQWGADLSGIDFQDIYYYLAPTDRAFDSWDDVPD
                     YIRRTYDRLGIPEAEKKYLAGVGGQYDSEVVYHNIREDLEKQGVIFLGTDEGLKRYPE
                     LFREHFGTVIPANDNKLAALNTAVWSGGSFIYVPKGVHVELPLQAYFRINSENMGQFE
                     RTLIIADEGSSVHYVEGCTAPTYSTASLHSAVVEIIVKKGARVRYTTIQNWSQNVYNL
                     VTKRAVAHEDAVMEWVDGNLGSKVTMKYPSIYLLGERAHGEVLSIAFAGDGQHQDAGG
                     KIIHAAPNTTSLITSKSISKGTGRTSYRGLVKVYPGCEKSKSTVRCDALILDEAARSD
                     TYPYMEIDEEDVTIGHEATVSKVGQEQLFYLMSRGLSEAEASAMIVNGFIEPIVKELP
                     MEYAVEMNRLIQLQMEGAVG"
     gene            19230..20486
                     /locus_tag="VFV59_00270"
     CDS             19230..20486
                     /locus_tag="VFV59_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEU5324299.1"
                     /translation="MTTATTPMPTAAALDAAAIFRISDLYGEPDWLRELRAAWWERAS
                     STPPPTGQEEEWRRTELAGLPRDGELLSDPPRVQITVSPAAAEAGVTVMDLRDAVRDR
                     PELVRRFLGRGDAQESHAHFWALAQAAWTGGLFCHVPAGVVLDETIVARTELPVGAHT
                     FLPVTLVVAEENSRVTLLEEIVSADGDATWYGGIAEIVAEPASEIRYARLQQLGDAAW
                     NIGAQRVVVGQDAVVTTLDAEVGSRVTKVGLDAQMTGKGGTSALLGLLAAGDDQHIDI
                     NAHQDLAADHTTSDLLYLSALYDRARAAFYGVTKVEPRTKQTSSYQECRNLLLSPNAG
                     AEPIPVLEIEANDILRCGHGATAGAIDPVQLFYAQTRGLDHDEAERLIVRGFFEQVVT
                     KVHDETIRSRVLAALARRIGTAEVEA"
     gene            20483..20827
                     /locus_tag="VFV59_00275"
     CDS             20483..20827
                     /locus_tag="VFV59_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEU5324300.1"
                     /translation="MTALDQRASGFVAVAHTGDVEPGTVRVVEVDGRSLCLGLTEDGE
                     WGAIDNVCTHDGGTLGEGELDDTAVECPRHGARFDLFTGRVLALPAVRPVRAYETVVD
                     GDEVRVRVEAGA"
     gene            20824..21309
                     /locus_tag="VFV59_00280"
     CDS             20824..21309
                     /locus_tag="VFV59_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5324301.1"
                     /translation="MKISTRAEYGIRVLVTLARTDDDRCLSLAEIARREKLPHAYLEQ
                     LVGDLRRAGLVTATRGQAGGYRLARAAEEIAMTDAVRALDGPLLEMPCAGTTDMEHCD
                     RPQPCSVHEVFQRVYESLSGTLAATTLAEVAATAGGPPYPPEVRRRRAATNTTLQASQ
                     S"
     gene            21330..>22052
                     /gene="sufC"
                     /locus_tag="VFV59_00285"
     CDS             21330..>22052
                     /gene="sufC"
                     /locus_tag="VFV59_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU5324302.1"
                     /translation="MTDQLVISGLEVSIAGKKILKGLDLTVAQGEVHALMGPNGSGKT
                     SLAYTLMGHPDYTVDAGSVTWRGGDLLTLSPDKRARLGLFLAFQYPSAIPGVTVASFL
                     RNIYNAVHHPKPAEAEGEAATKYTGIPLAKFRRLMEEKMALLRMDPSFATRYVNEGFS
                     GGEKKRLEMLQMAVLAPEMAILDETDSGLDIDALKNVAEGINASLSPEMGVLLITHYQ
                     RMLNYVKPQYVHVLMNGRIVMSG"
BASE COUNT         3169 a         7799 c         7902 g         3182 t
ORIGIN      
        1 gaacgggcag cgacagatcg gcatcaacgt cgtcctgaac caggacatca gcgacgccgt
       61 cgtgcgcgac atctcgcgct tcggccgcgt caacgacacc ttcccgcaga tcgacgcgct
      121 gaccatgaag gtgcgcgagg cggatctcgc ggccgtgcag gcgctgccct acgtggcagc
      181 tgcggcgccc gatgccaagc gcatcggcaa gccagtcccg acggccccga tggacgactt
      241 cgcggccggc atctccacct gggatcacga cgccatcaac gtcaccgact tcggtgccag
      301 tcggacgatc ggctacgacg gcgagggcgt gtatgtcgcg gtgctggata ccggcctgct
      361 gagcagctgg cgctactact tcggtgagga gcgcatcgcc gacgagtttg ccatcgcctt
      421 cggtggtggt ggcggtgagc gcggcagcgt gtccacccag cccaacaagt ggcagaccga
      481 ccaggacagc cacgggaccc acgtcaccag cacgatcctc ggctacaacc tccgcgacac
      541 ggccgtgaac ggggtggcgc cgatggccac tgtcatccca gtcaaggtcc tgaacaacaa
      601 cggatccggc tggtcgtcca tggttgccgc cggcatcgtg tacgtcgccg atctgaaggc
      661 gagcggtgca ctgggcgacg cgcccgtggt catcaacatg agtctcggcg gtcccgtcca
      721 ggagccggta gagaaagccg ctgtcgatta cgccatctcg aagggcgtca tcctcgtggc
      781 atccgctggc aacgagggtg aggccggcat gggatacccg ggcgcctatg ccccggtgat
      841 ctctgccgcc gcgtcaggct gggtcggcga gtgggcgcca ggcgccggct cgtggtggca
      901 gcgcgacgtc cccgatccga ctgacgtcga cgacttctac atcaccgatt tctcgagccg
      961 cgccatcaac gctcagcagg atctcgacgt cgccgctccg gggtcgtgga tcgtcggccc
     1021 gtatcaggtc aacggccagc tgagctacta ctacctcggc ggaacctcga tggccagccc
     1081 gcacgttgcg ggcaccgtcg ccctcatggc ccagaaggac ccgtcgctga cggcgacaca
     1141 ggcggagtcc atcctggaag gcagtgccat cgcgctcgcc gcgggctgtc gagacgtcat
     1201 cgatccgaac gtcggggact acgtcgaagt ctgctggggc agcgacgcca ccggacatgg
     1261 cctgatggac agcgctgccg ccgtggcggc cacgccgtag tcatctctcc cacgccgact
     1321 cgggaggccc cggctccggc cggggcctcc ggctatgcct gagacagcgc ggcgatgacc
     1381 tcgtacggat cggagccgac gatcggcgtg ccgtacagct ccatggcgcc gatgtccgag
     1441 gcccgctgca gcggatcccc gcggtcgacc atgcgcacga tcggggggat gtcgcgccac
     1501 ggtccgtccg gcgtggcacc cacgggcgga cgccacagcg cgaggttgaa cgatcggacg
     1561 cccatgcgat cgcggtaggc gaccagggtc gaggcgaccg catcggcgaa ggccgggtcg
     1621 cgttcgtcca tgccgggccg accgatcacc agcacctccc gctccttgat cggggtgagg
     1681 tgcgccagga ccgatacgcc tccggcgcgg tccgccgcca gctccaggtc acggtgcagg
     1741 gccacgaggt cgtccacgag tgacgcgtcg aacgaggcgc ggtaggcgat ggcgtcgcgc
     1801 tggaaacgag cgaggcgcgc gtactccgga ccacccccca acagcgcctg ggcgtggccg
     1861 tggacgatcg acccgccggc gcgccagagg cagttccaga tcagcaggta gttggccgcc
     1921 gacgggtcct ccgcgcgcgc caggtcggcc cacgcgcgac cggtggagat gacctcggcg
     1981 acgagctcgg catcgaaggc cagtgggtcg tgggcgtgga acacgaggac gccgtgatgg
     2041 gcatcggcga gggcggcgtt cgcgccggtc gtgatccgag gcccggtcac gcgcccgaaa
     2101 atgttccccg gcgtgcccgt ctccgggtcg cagaagggat cttccccggt cgtggcgagc
     2161 tgcgccgcca ggtcgtcacg gtgcgcgggt ccgtcgatcg gtcgatgggc gcggagcggt
     2221 gcgaacagcg ccgcctcgag cgtggcgagg ttcgtgacct tcacgatggt ctgcttccgg
     2281 accgcatcga ccgacccgaa cgtccgctgc agccagctga gcagctccgg cggcgggacg
     2341 gttcggccgt ggacgaggct cgcatccagc agccggtcgg cgcgttcgcg tgctgggccg
     2401 ttcagcgccg ccaggcgcgc cggcagatcg gtgatgccgg tcaggctccg tactcccagc
     2461 tccggcggtg ggggagcgcc gcccattcat cgcgaagcag gcccatgacg tggacgtcga
     2521 gatgctcacc ccgggcgaag tgggcgtgcc gcagtgtcgc ctcgtgccgg aaccccgcct
     2581 tctcgtagga ccggcgcgcc cggtcgttgc cgtcgtacac gtggagggag atccgctcca
     2641 gccggagctc gccgaagccg aagtcgcaga tggcccgcag cgcatcggtc ccgtagccgt
     2701 ggttccactc ggacttctcg ccgatggcga tgccgaactc ggcggtcccg ctgcgccagt
     2761 cgatgccgtg caacccgatg gtcccgatcg gccgatcgtc cgccaggagg cagatcacga
     2821 agtggtagtc ggtcttgccc tggcgggcga gcatgccctc gtaccactcg gtctcggatg
     2881 gctcgctcat cggcgcacgc atggccaggt tgtgggtcac ctcggcgtcg ttgaaccagc
     2941 gcacgaaggt cgggagatcg gtccgctcgg acgggcgcag gtagacctgc tcgccacgga
     3001 tgatcgggcg ccgttccggt aggctggaca tcggcccgag tctaccgaag gagaagtcca
     3061 ccccatggct gccacccgca ccgccaccgt cacctggacc ggcacgctcg cccagggctc
     3121 cggcaccgtc agcgccggca cgagcgagct gttcaccgat ctccccgtct cctgggcatc
     3181 acgcaccgag gaggcgaacg gtcagacctc gcccgaggag ctgctcgccg ccgcccacgc
     3241 ctcgtgcttc tcgatggccc tctccggagc gctgggccgc gccggcacgc cgccggagca
     3301 cctgcacgtc tcggcgaccg tcaccttcga caaggtgggc gagggatggg gcgtgaccac
     3361 gtccgagatc gacgtcgtgg gcgtggtgcc gggactcgac gaggccgcgt tccacgaggc
     3421 ggcccgcggc gcgggcgaca actgcccgat ctcgaaggcg ctcgccggca acgtccggat
     3481 cacggtcacg gcggcgctcg aggatccgca cgactgactc cccgcccggg gtgaaacctt
     3541 cgggctcctg gctgcctcgt tgaagatgcc ggccgctgcc gccggccaac gatcagatca
     3601 caggagcccg atccatgacc cgaccgtcca ccatccgcgg cgccgccctc gccaccggcg
     3661 cgctgctcgt cgccgccggc gtcgccttcg ccatgcccgg cccggaccgc accgatgcgg
     3721 aggacgacgt cgctgcggca tccgcctcgc cggtcgcaac cttcggtgcc gagctcgact
     3781 ccccgtcgcc gtccccgacc gcatccgcgg ctgatgacga tgggctcgag gacgaggacg
     3841 cggccgaggc gtcgagctcg cccgagctgg aggaactctt cgaggacgag tcggagagcc
     3901 cgtccgcgtc gcccgaggac gaggacggcg aggacgtcga cgaggacgac gccagcgcct
     3961 cggacgaggc cgaggcctct ccggacgacg gcgatgatga cgaggctgca ggcgacgatc
     4021 acgccgaggc ctcccccgac gacggtcagg atcacgattc cggcgctgac ggggaggacg
     4081 atgactcggg ctccggctcg gacgacagcg cccacgacga ctgaccgcct gcgtcggtgg
     4141 accgagccgc gagctcggcg tggcacgatg agggcgtgga cgacgccctc atcgccgcgc
     4201 tccgcgccgg ggatcgtgac gccttcgcgg cgctcgtcga gcgcgagacg ggaccgatct
     4261 accggacctg cctccgcatc ctgggcgtcg gtgcggatgc cgaggacgtc gcccaggaga
     4321 ccttcgtgat cgcgtaccgc tcgctgcgcg gctaccgggg cgccgggtcg ctgcgcggct
     4381 ggctgatgcg catcgccagc cgccaggcgt tccgccggct gcagcagcga cggccgtcgg
     4441 acccgctcga gggcgtggcc gaagagcgga tcgccgaccg aggtgccgac ccggcccgcc
     4501 tcacggttgc ccgggagcgg cacgggacgg tgcgcggcag cgtggcttcg cttcccgagc
     4561 cgtaccgaga ggtcgtggtg ctgcgcttct ttggtgagct ctcactgctg gagatcgccg
     4621 aggcgacggg ccggccgctc aacaccgtga agacgcacct gcgccggggc ctccagcgcc
     4681 tgcggccgct cctcggcgac gaggaggtgg tctcatgacc cggttcccgt tcggctccga
     4741 agagctgggt cgcaccgacc cggagctcga ccggatcggg gcgcagctcg agggctacgc
     4801 ggacgagacc cggtcgacgc caccggaagg cctggcgagc cggatcctgg ccgcggtgga
     4861 cgcagagccg gagccccggg tcggcgcctg ggcgcggttc agcgccgcgc tggtcgggct
     4921 ccggcgtccg gcgcaggcgc tggccgcggt ggcggtgttg ggggcggccg tcgtcggtgc
     4981 tctcgcgctt gctgagatcg ccgatcgcac ccggacggac ggcttcggtt cgtcatcgag
     5041 cccgctcgca tcgccggcgg tcacgccgtc gcccagcgcc acgccatcgc cgacgctggc
     5101 acccaccccc tcgccgacgc cgaccgccag cccgacgccc acgctgagcc cgacgccgga
     5161 ggcggtccag acgcccgacg cctccgccga tcaggagacg cccgagccat cagcctcgga
     5221 cgatgatggc ggcgacgacc acagcgggtc cggtggcggg ggcgacgact aggccgcctt
     5281 cagctccggg gaagggcctc ccagaccgcc agctcgttgc cctcgctgtc cgcgaagacg
     5341 ccgaaccagc ccatgccggg cacctcctgc ttcccggcaa tcacacgacc gccgagctcg
     5401 gggacccggg ccaggacgtc gtcgacggag cgcacgttga tgtacgtgcg catcgattcg
     5461 ggcgccatct cgccacgctt gccgatggcc ccgccgacgc cctgctcgcc gctgggcgtg
     5521 gtgaacaggt ggtagccgtc gaagccgggc gcgtcgctga agcgccagtc gaatagctcc
     5581 gcgtagaagc gcttggcgcg ctcgggctca tcggcgggga gctcgatgtg ggtgaagtcg
     5641 ccgtgttcca tgggtctctc cttgcgtcgc gtgccggcga cggtagcgcg gtcgtcacgc
     5701 gcgaagaagg gccaatgcgc cgaattcgtc gcactcggga gacggggact ggccactcgc
     5761 aggacgtgcg tcccggccac cacgacgcca gaaccgattc gatccggacg gcgtctatgg
     5821 cgcatgccga acgccgagct tcccttcctg attctcgccg tgctcgtctg gatcgtcgcg
     5881 ctcggcactc tgctgccgga acgctgatca gcgccgatac cgctcgagga tgtcgggcgg
     5941 gactcgcgtc gggcgcccgt cggagaggcg cacccacacc cagtgcgtca gcgcctccgt
     6001 cgtcagggcg ccgtccgact cccgacggat ctcggtgcgc cgcaggccgc ggacgccgct
     6061 gatctcctgc ggcagcgtgg tgaccagcac gatgtcgccg tagaccaccg gctggcggta
     6121 ggtgacgtgg tgctcgcgga cgacccaggc gccgccgcgg tcgagcgacc aggcgcgccc
     6181 gacccccatc gcctcgacgt ggtcgatcgc cacctgctcg atccagtgca ggtagaccgc
     6241 gttgttcacg tggcccagca ggtcgagctc gtgctggcgc acggagtagc gggaggtgaa
     6301 gcgccaggga tcgatgggca gaggggcatc ggtcacggtg tcgaggatat ccgccatacg
     6361 acgtagattc gacaggaccg atgcgctgcg gcgcggaccg ccgcagcgcc gatggcgaag
     6421 gacggtcgcg cacggacgat gacggtgaca ttcacccact ccacgctcac aggagtcatc
     6481 gctcgccatg gacgccgccg tcaccctgca cctcgccgag tcgccgaccg acctcgatgc
     6541 gttcgcgcgc tcgctgcgcg ggaccgtcgt gcgtcctgac gacgaggcct acgacaccga
     6601 tcgcctggtc tggaacgtcg cccaccaggg caacccgagc gtgatcgttc gggctgccga
     6661 tgcggccgat gtggcggccg ccatctcctt cgcctgtcgc aacgacctcg agatcgcggt
     6721 ccgttccggc gggcacagcc tggccggcca cagcagcggc gatggcaccc tggtcatcga
     6781 cgtgcgcgac atgaagggca tccacatcga ccccgaccag ggcacggtgt gggctggcgc
     6841 cggcctcacg gccggtgagc tgaccaacgc cctcgcggcg cacgggctgg cggtgccctt
     6901 cggcgacacc ggctccgtgg ggctcggcgg gatcacgctg ggcgggggca tcggctggct
     6961 ggtgcgaaag cacgggctga ccgtcgactc gctcgtggcg gtcgagatgg tcaccgcatc
     7021 cggcgaggtc gtcaccgcct cggagaccga gaacgccgac ctgttctggg ccctgcgcgg
     7081 cggtggtggc aacttcggcg tggtcacccg attccgctac cggacgcatc ggctcgacgg
     7141 catcctgggt ggcgcgatct tcctgccccc gacccgcgag gtgttccgca gcctggtgcc
     7201 gatcgccggc agcgcgcccg aggagctgac caccatcggc ttcgtcatgc cgatcccgcc
     7261 gctgcccttc gtcccggagg agcaccacgg gaagccggcg ctggcgctga tgttcgtgta
     7321 cgcgggcgat cccgaggccg gacgggcggc gatcgcgccg ttcacgcggg tcgcgacccc
     7381 gtacgccgtg gccgccatgc cgatgccgta cccgggcatc tacgagttca ccgccgaggg
     7441 atcggtggcg cacccgagcc ggaccaggtc ggtgttcatg gagacgttcg acgaggccgc
     7501 ggtcgagacc ctggtctcgg cgatcgagac ggcaccgccc gggtcgatgg tccagttccg
     7561 cgtcctcggt ggcgccatgg cgcgcgttgc tggtgacgcg accgccttcg cgcatcggtc
     7621 cgcggcggtg caggtcgtca tcgtcaacgc cttcccggag ccggggatgg aggaggcggg
     7681 ggttgcgtgg aacgagtccc tcttccgggc cctcgagccg agggcgagcg gcgtctacgt
     7741 gaacttcctg gaggacgagg gtgacgcgcg gattcgcgac gcctatccga acggcacgta
     7801 cgagcgactg gccgcggtca agcgccgcta cgaccggtac aacatcttcc accgcaacca
     7861 gaacgtgcgg ccggcctgaa ccggccacct caccccggac acgacgacgg cccggcggcg
     7921 atgccgggcc gttgctcatg cgcggaaggt ggagcgggaa acgggtctcg aacccgcgac
     7981 cttctgcttg ggaagcagac gctctgccaa ctgagctatt cccgcgcggt gcggctgatc
     8041 ctaccgcgac tgtcgcccgg ctgccaacgc ccacgccctc cgggtgccgg acagcgaacg
     8101 acgccggggc tcggactccc ggcgtcggtc gtagctgtcg gtggcgagcg tcagcgcacc
     8161 aggatgtgcg cgcctccacc gagggtggga tgggcggcga ccgcggtcgc ggcgccggcg
     8221 acggtcaggg cgaccgcggt cgcggctgcc gccacgcggg cgacggtgat gtgccggtga
     8281 gcctgcggga cgaagggccg ctgctcgtcg tgccggtccc gggcgacctg gcggatccgg
     8341 gccgcgtgcg ccaggcgctc gtcgtatgcc ttggttgcca tcagtcgcgt ggtctcgttc
     8401 gggccgagca tcggaacctc ctcttcgagc ctccccggct cgctgtgcgt tgggatgact
     8461 atcggcccga tccacgcaca ccacgatcgc gtgcgggaca tcggcccggt cattgatccg
     8521 atgcggccgc tgtcgcctcg cgctgcgttc gcctgtcgcc gcggcgacag gttgcgcgat
     8581 agttcacggg cggattgcac gaaacgcctt gacgccgcgc atcccgaggc gccacccttg
     8641 gcgccgctgc caacgcccgg tgcccactcg aggggaaatc agcagttgca cgacccgtcc
     8701 gaggttgtcg agggcctgcg tcgatgcgcg ttgttcgcgc acgtcgacga ggaagggctg
     8761 cgctcgctcg ccgggcggat gcgtcggcga cgcttcaagc gcaacgaggt catcttccac
     8821 cagggcgaca tcggtgactc gctccaggtg gtcggtgccg gaggcgtgaa gatcgtgctt
     8881 ccatcgccgg agggcgagga ggcgatcatc gccagcctga agcccggcga cttcttcggt
     8941 gagctcgcgc tcctggatgg ggccccgcgc tcgacgaccg cgacggcgat cgagccgacc
     9001 gagacgctca cgctgccgcg cgaggagttc ctgcggctgc tgtccgagga tccgcggctc
     9061 gtgcgcgcgt tactgcatgc cctcgccgag gagctgcgtc ggctcaccgg tcacgtcgag
     9121 gagctgcact tcctggacct ctcgggtcgt ctggcgatgc ggctggttcg actggcgcgg
     9181 gagaccgagc cgaacgcgac agccgatgtg cgcctggact ggccattcac gcagtcggac
     9241 ctcgccgcca tggtcggcgg cacgcgtcag tcggtgaacc ggctgctcag cggcctggtc
     9301 gacgatggcg tgatcgagat cgagcgcgac acgctggtga tccgtgacct ggacgagctc
     9361 gaggcgcgaa cggtccgatg acgtcggccc ggcggtgtca ccgatttgac agcggagctg
     9421 tccgcgcgcc gatgccgtcg ttggcacgcc ggcccataca ttccgaccca acagccatct
     9481 ggcaggccgc tggaaacaag gagatctgaa tggaccaata ccggcgacgc ccgccgatgg
     9541 aagggttctc gaggccggga tccctcggcg gagaggcttg ggacgatagt gatgacacag
     9601 tggagggtca catcatgagc gacgatcgca acctggacac cgagggccac atcaggcgcc
     9661 gcaccgacga cgagtcggac gtcgagggcc acggtttctc gctgggtcgg gcgaccgagg
     9721 acgacgacac cgagggccac ggcttctcgc tgggtcgggc gaccgaggac gacgacaccg
     9781 agggtcacgg ctacagcctg ggtcgtgcga ccgaggacga gccggacacc gagggtcacg
     9841 gctacagcct gggtcgggcg accgaggacg acgacaccga gggccacggc ttctcgctgg
     9901 gtcgtgccac cgaggacgac gacgacaccg agggccacat cttcgtgagg taaggcgtgg
     9961 ccgatcagcc acccgcagcc gcgctcgccg ccctcgtggc ggcggcgcgg cgttctgcca
    10021 tcgggaggcg cgtccagggg gacgtcgaga cccgcctcct ccagagcatc gtggacgcca
    10081 ccgcccgact gttcgacgcg gaggcgtcct cgatcgcgtt gttcgagcgt gatccggatc
    10141 ggctcgagtt ccgcgtcgcc gccggagcgc agggcgccgg cgccatcggc ctgaccgtgc
    10201 cgcccaccaa ggggatcgtg gggttcgtct tctcgaccgg ccaggcgatc gcgctctcgg
    10261 acgtcaccag cgacccgcgc ttcgacaaga ccgccgcgca gcgaaccggc tacgtcccac
    10321 gctcgatcgc cgcggttccg ctgctcgacg agggcgaccc ggtcggcgtc ctgcaggtgc
    10381 tcgacaagcg cggctcgccg accttctcgc tccaggacat ggagctgctc ggcgtcttcg
    10441 ccgggcaggc caccgtcgcg atcaacgccg cgcgggtcca gcgagacacc acgcggctga
    10501 tgcgcgccgt gctcacggcc atcggtcccg acctggcgcc cgacgacatc gaggcggtcg
    10561 tctcgtccgt cgcggccgag ctggacgtcg acgaggaggc gcccttctgg cgcgtcgtgg
    10621 accaggtcgc tcggctgcgg tccctcagcg accgtgaggc ggcgctcgtc gccgacatcc
    10681 tggaggtgct ctccgcccac gccgcccggg cgcagcgcgg ccgcggctcg cgatgaccgt
    10741 gctcggtgag cgcgccggga tgagcgtgcc cgaatgagcg agcccctgct tcccgcctgg
    10801 tcggagccct tcgcggaccg acagcgcggc ctgcgccgcc gagcgcggct gcgtggcgtc
    10861 acgcgtgact gggcctgggg cggcgcgacg ggcgccggga tccgcgtcgg gatcatcgac
    10921 tccggcctcg agaacgacca cccacgcctt cgcggccgcg tggtccagaa cgtggccgtc
    10981 gagatggggc ctgatggacc ggtggtggtg gatgacgagc cccgcgacct gttcggccac
    11041 gggaccgcct gcggttccat cgtgctcgac ttcgcgccgg aggccgagct ggtctcgatc
    11101 cgcgtgctcg gtgccgacct ccgcggcaag ggcacagcct tcgccgccgg cctggagtgg
    11161 gccatcgagc aggggctgca cgtctgcaac ctcagcctct cgtcgaagag tgagtccctg
    11221 tatccggtct tccacgacct ggtggatcgg gcctacttcg cgtcgatcgc gctggtgagt
    11281 gccgcgaaca acgtccccgc cccgagctac ccgtcgctgt tcgcatcggt cttctccgtg
    11341 gcggcccatg gggagccgga tccgtggcgg atctactaca acccgtcccc accggtcgag
    11401 ttcggggcgt ggggcgtgga cgtgccgatc gcctggaagg acgggggcag caccgtcgcg
    11461 accggcaaca gcttcgccgc gccccacgtc gcggggatcg tggcgcgcat ccggtcgaag
    11521 cacccggagc tcaccccgtt cgagatcaag gccatcctcg ccagcgtcgc cgacaacccc
    11581 gggcgccggc atccgtggcg ccaccggacg ccgtccgccg gatgaagctg gcgcggacct
    11641 acctggacct gttccggcgc aacccgtcgc tcgcgcggct gctggccggc gagttcatct
    11701 cgggcatcgg cgactggctg tacctcgtcg ccatcctggt cgtggtctac gcggagagcg
    11761 agtcgccggt cctgctgggc atcgtcgggg ccgctcgcat cctcccgtac gtgctgctct
    11821 cagtcccggc cggcatggcg gcggatcggt tcgaccggcg catggtgctg ctcgtgaccg
    11881 atatcgcccg cggcatcctg atggtcgtgc tcgtcgtgct ggtgatcgtc ggggcgccga
    11941 cgctcgccat catcgccgtc tcggtcctgg ccgcctgctt ctcgaccttt ttcggacccg
    12001 ccatcgcgtc cctgctgccg atgctggtcg acgagcgcga tctggggccc gcgaactcgg
    12061 cgtgggccac gctcgacaac atcgcgttca tcgtcggacc ggccgttgcc ggcgtcctgc
    12121 tggcgagcgg cgggctcgag ttcgccttcg cgctcaacgc cgtctcgttc gcggtcgtcg
    12181 ccatcgtgct gtggcggttg gaggtgccgc agcgcaaggt gccgacggcg accgccccgt
    12241 cggacgaggc cccacccccg cagcagggat tccgcgacct gctgcggccg ctggcgggtc
    12301 cgttcgtcct ggacgccgcc accagcatcg tgggcggcgg gatcagcgtg atgacggtcg
    12361 tgatcgccgt cgacgtgctc ggcgccggcg aggccgggac gggatacctg aacgccgcga
    12421 ccggggtggg tggcgtcgtg gccggggtgg caggcggcgc cctgctcgcg cgccgcctgc
    12481 acgtgccgct gctgctgggt gggatcgtcg gtggcgcggg gctggcggcg ctcgccctga
    12541 ccggcgacct ggccttggcc atggtggcca tcggcgtggc ggtcgccggg ctcctgctgc
    12601 ttgagatcgt caacaccacg ctcatccagc gcatcgtccc ggaccagctg cgcgggcgtg
    12661 ccatgggcat cctgcagacc agctccgcgg tgctctacgc cgggggatcg ctcgtcatgc
    12721 cactcctggc cgatgccatc ggcgtcaccc cggtcctcgt cggggccggc gcgctggtcc
    12781 tgcttggcgt ggtcggcgcc ctgctcctgt ctcgcgaggc cgaggaggcc gcgcccatcg
    12841 gcgtcgagcg ctcacggctg ctcgagcatc cgatcttcgc cggcctgccg acgccgcgcc
    12901 tcgaggctgc ggctcgcgac ctgggcgagg tgcccatggc cgccggcgac gtggttgtcc
    12961 gccagggtga cgcggccgac cgcttctacc tgatcgcgga ggggacgctg cgcgtgactc
    13021 agacgcgcga cggcaagcaa gtcgagctgc gcacgctcgg tccgggcgac gtcttcggtg
    13081 aggtcggcct gctgaaccga tcgccacgca cggcgacggt cacggcgctc accgacggcc
    13141 ggctgctggc cctcgatggc gaagacttcc tggagctggt gagctccggt ccagggctgt
    13201 cgagccggct cctggacctg taccggggtg ccatcagtcg ctgacggcgt ccggctggga
    13261 ttgaacgcgc gagtcgttgc gcgcgtatcg gtcacggatg ggtcacctcc gttcgcgacg
    13321 cctcgtcccg ctgctgctgc tggcgctcgc cggctgtgcg tcgggcacgg cccaaccgac
    13381 cccggtctcc ttcgaggccg atccatccgc catccggaac cgccttgccg ggttgcagga
    13441 gatcgccgac gcgaacgacg gcgtccgcac ggtcgggacg gtggggtacg aggcatcggt
    13501 cgatcgtctc gcggccgacc tcgtcgagct cggctacgcc gtggacacgc cgcaggtcga
    13561 cttcaccggc ttctccgacg agggctcacg cgtcgaggtt ggcaatcgga cgttctggag
    13621 cccggacgag gtgaaggccc tcatctacgc ggcggccggt gacgttaccg ggccggtcgc
    13681 catcctcgat gggagcggtt gtgacccggg tgacttcgcg ggcgtgccgg agggcgccat
    13741 cgtcgtcacc accggcagcg gctgctatcg gcggcagcag gccatgaacg cggccgacgc
    13801 gggggccgtg gccttcgtgg tcggctaccc ggaccgggga gcggcggaga tctaccgccc
    13861 cacgctgatc gacccgggcg ggatcacgct tccggtggtc agcgcgaccg gcgacgccgt
    13921 gcaggccctg cgccgggccg gaggggagac ggcgcgcgtc gtaaccgaca cccggcgcga
    13981 cccgtcgacg ttccgcaacg tggtggcgac actcgggagc ggctcgtcgg tcctcgtcat
    14041 cggtgcgcac ctcgactcgg tcctcgacgg ccccgggatc aacgacaacg gcagcggcgt
    14101 ggcggccgtg ctcgaggtgg cccgcgggct ccaacagcac ggcgttccgg atggctggcg
    14161 ggtcgtgatc ggcctgtggg gcgccgagga gttcggcgac atcggctctc gcgcctacgc
    14221 cgacgccgcg ggcgacggcg ttgaggcgta cctgaacctg gacatggcgg ggtcgctcaa
    14281 cggcgccacc ctggtctacg acgacgccga tgccgcgccc ggctccgagc gcctcaccga
    14341 gctctacggc acctggctca cggcgcaggg cgagccgtac caaccggtcg atctgagtgg
    14401 ttccagcgac cacgccagct tcacggcgct gggcatcccg accggcgggc tcttctccgg
    14461 cgcctcggag accgggccgg cctcgcgtcc gggtgcatcg gcgtcgggcc cggtgccgga
    14521 cccctgctac cacctggcct gcgatgacct cgacaacgtc gacatcgagc gcgtcgtcct
    14581 cttcgccgac gcgacctacg cggtggcgtt gcagctcatg acgggcgagt gatcaccccg
    14641 ccggcggcat ccggcgcatc ggtgaagcca tccgcagcgt caggaggcca ggtcgccggt
    14701 gagcatgcgg cggtaggagt cgtatcggtc ctcggagacc tcgcccgctc ccaccgcggc
    14761 gcgcagtgag cagcggggct cgtgcaggtg cgtgcagtcg ttgaaggcac agccgcccag
    14821 gtgtgcgcgg aactcgggga agcaccacgc cagctcggtg gaggggatct cccacagtcc
    14881 gagctcgcgg atgccgggcg tgtcggccac gtagccgccg gctgcgtcgt cgatcggcag
    14941 cagctcggcg tgcgtggtcg tgtggcggcc cttgtgcagc acctcgctga cctcgccggt
    15001 cgccaggcgc aatccgggct gcatggcgtt gagcagcgtc gacttgccga cgccggaggg
    15061 tccggtgacg atcgagaggc ggcccgccag ccgctggtgc agctcgtcca ggccgatccg
    15121 cgccttggcg ctggtgtaga cgacgtcgta gccgatgcgc tggtaggcgc cgaagatctc
    15181 ttcggcggcg ccgcgtccga cgaggtcgac cttgttcgcc acgatgagcg tgggcacctc
    15241 gttgtactcg gccaccacca ggaaccggtc gatcaggcgc gggttcggcg gcgggtgggc
    15301 gcacgcgaag acggcagcca ccaggtccgg gttggccacc agcacgtctt cgcgccacga
    15361 cccgcgcggg ccgggttggc ggcggctgaa caccgcctcg cgcggctcga ccgacgcgat
    15421 cgcgccggtc ccgtcgggga ggtgctcgac cctcacccgg tcgccgatga cgacgatgtc
    15481 cgcgctctgg cgctccttct tgagccggcc gcgcagccgc gcctcgacgc gctcgccgtc
    15541 atccgtcagg accgtgacga accccgatcg ggatcgcagc accaggccgc tggcggtctc
    15601 ggtcacacgc cggtcatccg agtgcgccca ccacgctggc gtagacggtg tcctggcggc
    15661 ggggaagctc ctcgccgcgg gccatccgac cgtcccacgg gtcgacgccg atgccgagct
    15721 gggacagcca cgccatgccg ggccggttgc tggtgggcag gttgatggcg agctcggtcg
    15781 cctgcggcgc gcgcgcgaag gcatcgcgga ccagcccctg ggcgatggtc gggtcatcgg
    15841 ccaggagcgg cccgacgcgc tcaccgtccg gccggagcca ggcgtagccg gcgatgccct
    15901 cgtcgcgctc cgcgatcgcg acgggtcgct ccgggtcggc cagcatgcgg accagcagct
    15961 cagaccgatc tgcgccgaat cgcggctcgt cgtaggccgc caggcgcgcg aggtcggccg
    16021 ggccgccacg ccggatcgcg ttgggaccgt cgtcggtcgc atccgcctcg aaggcggcac
    16081 ggggcagccg gacgtgcgaa ccgggctcga ccagggcgaa cccgtagcgc tggtacagcg
    16141 gacgaccgtc cgtcgtggcg tacagctcct ggcgcacgca tccagcggct gagaggtggc
    16201 cgaggatggc ctccaggatc tgcgcgccga ggccccgacg ccggtgctcc ggggcgacga
    16261 tcatgttgcc gacgacgccc aggcgcccgt agaccatccc ggagccggag gcgatgacgg
    16321 caccgtcggc gtcttccgcc acgacgtaga tgcctcgcgt gctctccagg acggcgcgga
    16381 gggcccagtc ctgcacgccc cacccgaccg actcgcgcag ggcggcgagc gccggcaggt
    16441 cgtccggcgt cgcatcgcgc agcttcatcc cggtcgcagg cacgtcgccg agtctacccg
    16501 ccggtagcat gcggctcgtg gaacgcgaga tcgacgtgct gctggtgggg gccggggtgg
    16561 cctcggcccg gtgtgcgcgc acgctgcgcc gcaacggctt cgacggctcc atcctgctgg
    16621 tgggcgacga gcccatgccg ccgtacaacc gcccgccgct gagcaaggag ctgctgcgcg
    16681 aggacctgcc cccggagctg ctgatggcgg agccggcgtc gttctacgag cgccgggcgg
    16741 tcgaggtgcg caccggcgtg accgtgacgt ccctggacgc cgagacacgc agggcgacgc
    16801 tcgaggacgg cagcaccctg cgctacggcc gctgcctgct cgcgaccgga gcggagcctc
    16861 tgcggctgcc ggtcccaggc ggcgatcttg gcctgcccct tcgcaccgtg gcggacgcgc
    16921 agcggctccg cgcccgcgcg caggatgcgg acccggggac gaccgtggcg atcgtcggcg
    16981 gtggcctgat cggggtcgag gtggcgtccg gcctcgcggc gctggggctg cggccgacga
    17041 tcctggagcg ggcgggcgcc ctgtgggccg gttccctggg ccacgagctg gcggcgtggg
    17101 cggccgcccg gctggccgac gccggcgtcg tcacccgcac cgacgcctcg gtcaccggga
    17161 tcgagcccgg cgcggtgctc gtgggcgagg agcggatgga ggcgcgattc agcgtggtcg
    17221 gcatcggcgt gcgaccgcgt gtcgcgatgg cggcggcggc cgggctggtg gtggacgacg
    17281 gcatcgtggt cgacgcggag cagcgaacct cggacgagcg catctgggcc gccggcgacg
    17341 tggcgcgcac ggccgggcgt cgggtcgagc actggcacgc ggcccgggaa gcgggggagc
    17401 gcgcggccct ctcgatgctc ggtcggcccg tgccgccgct tcccgtcccg tggaccttca
    17461 ccgaggtcgc cgggacgccg gtcgacatcc tgggcacgcc cgacgattgg gacgaggagc
    17521 gctggatcgg tggcggccgg gtgctggcgt atcggtccgg cgctcacgtc gtgcagctgg
    17581 cggtcatcga cgccgccctg gatcccgggc gcgcgcgcgc actggtggcg gcgcccgcat
    17641 cggtcgcgga ggtggagcgc gagttcacgg cgaacagctg aggctggcag acggcaggcg
    17701 gttcggcccg cttcggcggg cccttcggtc ggttgaccga ccctcgacgc tgcgggacaa
    17761 tggcggcggt aaaccgacca aatcagtcgg gattgtgtcc cgaagacccg cgaggagcgc
    17821 atggcccttc ccaatcctgc cctcagtcag atcccgaccg gctacaagta cggctggaac
    17881 gaccccgagg cgaagtcgat caacatcgcc cgcaagggcc tctcggccga cgtcgttgag
    17941 gagatcagcc gcttcaaggg cgagccggag tggatgatga agctgcgcct caaggcgtat
    18001 cgccacttcg aggcgcgccc gatgccgcag tggggcgccg acctgtcggg gatcgacttc
    18061 caggacatct actactacct ggcgccgacg gatcgggcct tcgactcctg ggacgacgtg
    18121 ccggactaca tccggcgcac gtacgaccgc ctcggcatcc ccgaggccga gaagaagtac
    18181 ctcgccggcg tcggcgggca gtacgactcg gaggtcgtgt accacaacat ccgggaggac
    18241 ctcgagaagc agggcgtcat cttcctgggc accgatgagg gcctgaagcg ctacccggag
    18301 ctgttccgcg agcacttcgg cacggtcatc cccgcgaacg acaacaagct ggccgcgctc
    18361 aacaccgccg tctggtcggg cggctcgttc atctacgtgc cgaagggcgt gcacgtggag
    18421 ctgccgctgc aggcgtactt ccgcatcaac agcgagaaca tgggccagtt cgagcggacg
    18481 ctgatcatcg ccgacgaggg ctcgagcgtg cactacgtcg agggctgcac cgcgccgacg
    18541 tactcgaccg cgtcgctgca ctcggccgtg gtcgagatca tcgtcaagaa aggcgcgcgg
    18601 gtccgctaca ccaccatcca gaactggagc cagaacgtct acaacctggt caccaagcgt
    18661 gcggtggccc acgaggacgc cgtcatggag tgggtcgacg gcaacctcgg ctccaaggtg
    18721 acgatgaagt acccctccat ctacctgctg ggtgagcggg cgcacggcga ggtgctgagc
    18781 atcgcgttcg ccggcgacgg ccagcaccag gatgcgggcg gcaagatcat ccacgcggca
    18841 ccgaacacga cgagcctgat cacctcgaag tccatctcca agggcaccgg ccgcaccagc
    18901 taccgcggcc tggtcaaggt ctaccccggc tgcgagaagt cgaagtcgac ggtccgctgc
    18961 gacgcgctga tcctcgacga ggcggcgcgc tcggacacgt acccgtacat ggagatcgac
    19021 gaggaggacg tcaccatcgg ccacgaggcg accgtctcca aggtcggcca ggagcagctc
    19081 ttctacctga tgagccgcgg cctgtcggag gccgaggcca gcgccatgat cgtcaacggc
    19141 ttcatcgagc cgatcgtgaa ggagctgccg atggagtacg cggtcgagat gaaccgcctg
    19201 atccagctcc aaatggaggg ggcggtcggg tgacgacggc caccacaccg atgccgaccg
    19261 cggccgccct cgacgcggcc gcgatctttc gaatcagcga tctctacggc gagcccgact
    19321 ggctgcgcga gctgcgcgcc gcgtggtggg agcgcgcgtc ctcgacaccg cccccgaccg
    19381 ggcaggagga ggagtggcgc cgcaccgagc tggcggggct tccgcgcgac ggtgagctgc
    19441 tgagcgaccc gccccgcgtg cagatcacgg tcagcccggc cgccgcggag gccggcgtga
    19501 cggtcatgga cctgcgcgac gccgtgcggg accgcccgga gctcgtccgg cgtttcctgg
    19561 gccgcggcga cgcccaggaa tcccacgcgc acttctgggc gctcgcccag gcggcctgga
    19621 ccggcggcct cttctgccac gtcccggctg gcgtggtgct cgacgagacg atcgtcgcgc
    19681 gcaccgagct gccggtcggg gcgcacacct tcctgccggt caccctcgtc gtggccgagg
    19741 agaacagccg cgtcacgctg ctggaggaga tcgtttccgc cgatggtgac gccacgtggt
    19801 acggcgggat cgccgagatc gtggcggagc ccgcatccga gatccgctac gccaggctgc
    19861 agcagctcgg cgacgcggcc tggaacatcg gcgcgcagcg cgtcgtggtc gggcaggacg
    19921 cggtggtgac gacgctcgat gccgaggtcg gctcgcgcgt cacgaaggtc ggcctggacg
    19981 cccagatgac gggcaagggc ggcaccagcg cgctgctggg cctcctggcc gccggcgacg
    20041 accagcacat cgacatcaat gcgcaccagg acctggcggc cgaccacacg accagcgacc
    20101 tgctgtacct ctccgccctc tacgaccgcg cgcgcgccgc cttctacggc gtgaccaagg
    20161 tcgagccgcg gacgaagcag acgagctcct accaggagtg ccggaacctc ctgctctccc
    20221 cgaatgccgg cgcggagccg atcccggtcc tggagatcga ggccaacgac atcctgcgct
    20281 gcggtcacgg ggcgacggcg ggcgccatcg atccggtcca gctgttctac gcccagaccc
    20341 gcgggctcga ccacgacgag gcggagcgcc tgatcgtgcg cggcttcttc gagcaggtcg
    20401 tcaccaaggt ccacgacgag accatccggt cgcgcgtcct cgccgcgctg gcgcggcgca
    20461 tcggtaccgc cgaggtggag gcatgacggc gctggaccag cgggccagcg gcttcgtcgc
    20521 ggtcgcccac accggcgacg tggagcccgg caccgtccgc gtggtcgagg tcgacgggcg
    20581 ctcgctgtgc ctgggcctga ccgaggacgg ggagtggggc gccatcgata acgtctgcac
    20641 ccacgatggc ggcacgcttg gcgaggggga gctggacgac accgccgtcg agtgcccccg
    20701 ccacggcgcc cgcttcgacc tcttcaccgg tcgcgttctg gcgctgccgg ccgtgcgacc
    20761 ggtccgcgcc tacgagaccg tggtggacgg tgacgaggtg cgggtgcggg tggaggcggg
    20821 cgcatgaaga tcagcacccg cgccgagtac ggcatccggg tcctggtgac gctcgcccgg
    20881 accgatgacg atcggtgcct ctcgctggcg gagatcgcgc ggcgcgagaa gctgccgcac
    20941 gcctacctcg agcagctcgt gggcgatctg cgccgggccg gtctggtcac cgccacccgc
    21001 ggacaggccg gtggctaccg gctggcgcgc gcggccgagg agatcgccat gaccgacgcc
    21061 gttcgcgccc tcgacggccc gctgctcgag atgccgtgcg ccgggaccac cgacatggag
    21121 cactgcgacc gaccgcagcc gtgcagcgtc cacgaggtct tccagcgcgt ctacgagtcg
    21181 ctctccggga cgctggcggc caccacgctg gccgaggtcg ccgccacggc gggcgggccg
    21241 ccgtaccctc ccgaggtccg tcgccgacgc gcagccacga acacgaccct ccaggcatca
    21301 cagtcctgac gcagcacacg aaggaaccca tgaccgacca actcgtcatc tccgggctcg
    21361 aggtgagcat cgcggggaag aagatcctca aggggctcga tctgaccgtc gcgcagggcg
    21421 aggtccacgc cctcatgggc ccgaacggct ccggcaagac cagtctggcc tacacgctca
    21481 tgggccaccc cgactacacg gtcgacgccg gcagcgtcac gtggcgcggg ggcgacctgc
    21541 tgaccctctc gccggacaag cgtgcgcggc tgggcctctt cctggcgttc cagtacccgt
    21601 ccgccatccc gggcgtcacc gtcgccagct tcctgcgcaa catctacaac gccgtgcatc
    21661 acccgaagcc cgccgaggcg gagggcgagg cggccacgaa gtacaccggc atcccgctgg
    21721 ccaagttccg ccggctgatg gaggagaaga tggcgctgct gcgcatggac ccctccttcg
    21781 ccacccggta cgtcaacgag ggcttctcgg gcggtgagaa gaagcgcctg gagatgctcc
    21841 agatggcggt cctggcgcct gagatggcga tcctggacga gaccgactcg ggcctcgaca
    21901 tcgacgcgct gaagaacgtg gccgagggca tcaacgccag cctctcgccg gagatgggcg
    21961 tgctgctgat cacccactac cagcgcatgc tcaactacgt gaagccgcag tacgtgcatg
    22021 tgctgatgaa cggacggatc gtgatgagcg gc
//
LOCUS       DASTYP010000005         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_146164, whole genome shotgun sequence.
ACCESSION   DASTYP010000005 DASTYP010000000
VERSION     DASTYP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146164"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VFV59_00290"
     CDS             <1..1429
                     /locus_tag="VFV59_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5324303.1"
                     /translation="LAAIAVMDGGQDLPPGAAALDDLVSADVPPEWHDRWQARWRALT
                     RDDVATIVHTSGTTDNPKGAILTHGNLVAVCEGSPQALPFLESDIAMSVLPLSHIAER
                     AGGQFVPLAIGASVTFAEPIIERWPANLAEVRPTAMVTIPQFFSRMYRRVQDTLEAGP
                     AWKRRAFDWATGLGVQRYANHLAGRADSPWLRLQLWLAGRLVFRPLKERVGGRLRFFL
                     CGAAPLNPDIGRLFYAMDMLVLEAYGLTETASAVCMNTPQNFQFGTVGTPFPDMEIRI
                     DAESGEVLIRGPQVFRGYLNLPEETAAALDGEGWFHTGDVGQLDDEGRLTITDRIKNI
                     LVLSNGKKVMPGPMENALDASPFIEQSVILGDHHAQTGVLVVPDAAYLAAWAAKQGIK
                     ESSLEALSRSEAVADLIEAEVRRLLGDFAAWERPRRVVVLPRAFTEEDDELSLTRKPK
                     RRVIVEHFPEQVARLFEGERMEATA"
     gene            1426..2796
                     /locus_tag="VFV59_00295"
     CDS             1426..2796
                     /locus_tag="VFV59_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324304.1"
                     /translation="MTAQVRDRSALPLADKLLYATACIGNNAMFFAQVTLVAVFYSGT
                     ANAPERLAPTLVAIALGIGKFIELFDDPLIGWWSDATKSRWGRRIPFILLGTPIIGIS
                     FWLLWTPPPPDAGWLNLVWFVVVIEVFFLARTVVEAPYEALQAEITTSPTERVSLGAY
                     KVAFSFVGAALGMVAAPLLVELVGYSGMGLVLAVVSVTAIYTMLFGLWRRGTLDTGTG
                     PVERPPLFGSLVGTLRNANFRALAASFLFFSLGFQLLTLMLGYFVTRVLGVSDGAIGA
                     FTAGVIVFAVIALPMLNRLARRFTKRVLYAGAMLLLSAYLLVASIGIFVQILPGVMFF
                     TQTLILISLAGLGFGAMWIYPGAMVADIIDEDRRQYGEGRSAVFYGMYKTLEKFAQAG
                     ALIVGGISFALFGDTSANPLGIQLLMPLAAGAIFLGFVAVWIGYHVRESPGARPGLTE
                     AAVAAG"
     gene            complement(2793..4196)
                     /locus_tag="VFV59_00300"
     CDS             complement(2793..4196)
                     /locus_tag="VFV59_00300"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5324305.1"
                     /translation="MTDALRPHLDLDACITHLRDLIRIPSVNPPTGGDDVAAGRDPAG
                     GETAAATYCADVLRDAGIEAEVLEPTTPGRGSCVARLRAEGPASEPPLILLSHIDVVP
                     VDAEAWTRDPFGAELVDGVVWGRGAVDMKNMVAMELGVMLALARSGVPLRRDVIFATV
                     ADEEAGGEHGARALVEQRPDLFADAQGRPAAAAINEVGGYSMTIGGRRFYTVQVAEKG
                     IAWTRLRSTGTPGHGSMPHPDNAATRLAAAVTALVEDQRQRPARVHPVVGAFLSRLGL
                     DEVAAMVEDDPAGASVRLEALVEDPVLRRSIEAMLRDTVTPNVMRAGKKVNVIPSSGE
                     AEVDVRTLPGTDQDAFLRHLQEVVGDLATVEPVVQLPSVECDPDAEIVTLMHDALRRA
                     DPDATSVPMMITPGTDAKALATLGIPCYGFAPLQLAPDVPFLSLFHGHDERVPVSAIA
                     FGLPVLAEVVERYVVRP"
     gene            complement(4202..>4769)
                     /locus_tag="VFV59_00305"
     CDS             complement(4202..>4769)
                     /locus_tag="VFV59_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324306.1"
                     /translation="EQGWFGVPAGNFYSWLFVTLAFSLFTRWLRGRAHTRPMLDWLQL
                     LVPIPAFGVLIASIGAFAIVKPMTDPTRGGGLAVFWVVLAAFVAISVWAVLGPDRGRA
                     DGAADAILDLRLAFASRLAIHLVFLAALVWLGLAVSEPVLLLVALAMLALELPLGIAA
                     HRRGLTAAASARSAPREATVRTASEPLP"
BASE COUNT          665 a         1680 c         1709 g          715 t
ORIGIN      
        1 gctcgcggcg atcgcggtga tggacggcgg tcaggacctg ccgcccggcg ccgctgcgct
       61 ggacgacctc gtctccgcgg acgttccccc cgaatggcac gatcgctggc aggcgcggtg
      121 gcgagcgctc acccgcgacg acgtggccac catcgtccac acctccggca cgacggacaa
      181 cccgaagggc gcgatcctga cccacggcaa cctcgtggcg gtgtgcgagg gatcgccgca
      241 ggcgctgccg ttcctcgagt cggacatcgc catgtcggtg ctcccgctga gccacatcgc
      301 cgagcgggcg ggcgggcagt tcgtgccgct cgccatcggc gcatcggtca cctttgccga
      361 gccgatcatc gagcgctggc cggcaaacct ggccgaggtc cggccgacgg ccatggtcac
      421 catcccgcag ttcttctccc gcatgtaccg ccgggtgcag gacacgctcg aggccgggcc
      481 ggcgtggaag cggcgcgcgt tcgactgggc cacggggctc ggcgtgcagc gctacgccaa
      541 ccacctcgcg ggacgtgcgg actcgccgtg gctgcggctg cagctgtggc tcgcgggccg
      601 cctcgtcttc cggccgctca aggagcgcgt cggcgggcgg ctgcgcttct tcctgtgcgg
      661 cgctgccccg ctcaacccgg acatcggccg gctgttctac gccatggaca tgctcgtgct
      721 ggaggcgtac gggctgaccg agacggcatc ggcggtgtgc atgaacacgc cccagaactt
      781 ccagttcggg accgttggca cgccgttccc ggacatggag atccggatcg acgccgagag
      841 cggcgaggtg ctcatccgcg gcccgcaggt cttccgcggc tacctcaacc tccccgagga
      901 gacggccgcg gcactcgacg gcgagggctg gttccatacc ggcgacgtcg gccagctcga
      961 tgatgagggg cggctgacga tcaccgaccg gatcaagaac atcctggtgc tgtcgaacgg
     1021 caagaaggtg atgcccgggc cgatggagaa cgcgctcgac gcctcgccgt tcatcgagca
     1081 gagcgtgatc ctcggcgatc accacgccca gacgggcgtc ctggtcgtcc ccgatgccgc
     1141 gtacctcgcc gcgtgggcgg ccaagcaggg gatcaaggag tcgagcctcg aggcgctgag
     1201 ccgaagcgag gccgtggcgg acctcatcga ggcggaggtg cggcgcctgc tgggcgactt
     1261 cgccgcgtgg gaacgcccgc gccgggtggt ggtgctgccg cgcgccttca ccgaggagga
     1321 cgacgagctg agcctgaccc ggaagcccaa gcgtcgggtg atcgtcgagc actttcccga
     1381 gcaggtcgcc cgcctgttcg agggcgaacg aatggaggcc accgcatgac cgcgcaggtc
     1441 cgcgaccgct cggcgctgcc gctcgccgac aagctcctct atgccacggc gtgcatcggc
     1501 aacaacgcga tgttcttcgc gcaggtgacg ctggtcgccg tcttctactc cgggacggcc
     1561 aatgcgcccg agcgactggc gccgacgctg gtcgccatcg cgctcgggat cgggaagttc
     1621 atcgagctgt tcgacgaccc gttgatcggc tggtggagcg acgcgacgaa gtctcgctgg
     1681 ggccgacgca tcccgttcat cctgctcggc accccgatca tcgggatcag cttctggctg
     1741 ctgtggacac cgccgccgcc cgacgccggc tggctgaacc tggtgtggtt cgtcgtggtc
     1801 atcgaggtct tcttcctggc ccgcaccgtg gtcgaggcgc cgtacgaggc gctccaggcg
     1861 gagatcacga ccagccccac cgagcgtgtc agcctgggcg cctacaaggt ggccttctcg
     1921 ttcgtcggcg ccgcgctcgg catggtggcg gcgcccctcc tcgtcgagct ggtcggctac
     1981 agcggcatgg gcctcgtgct ggcggtcgtc tcggtgacgg ccatctacac gatgctgttc
     2041 ggactgtggc gtcgcgggac cctcgacacc gggaccggcc ccgtcgagcg gccgccgctg
     2101 ttcgggtccc tggtcggcac gctccgcaac gccaacttcc gtgcgctcgc ggcgagcttc
     2161 ctgttcttca gcctcgggtt ccagctgttg acgctgatgc tgggctactt cgtgacgcgg
     2221 gtgctcggcg tgagcgacgg cgccatcgga gccttcaccg cgggcgtcat cgtcttcgcc
     2281 gtcattgcct tgccgatgct gaaccggctg gcccggcgct tcaccaagcg cgtgctgtac
     2341 gccggggcga tgctgctgtt gtccgcctac ctgctggtgg catccatcgg catcttcgtc
     2401 cagatcctgc ccggcgtgat gttcttcacg cagacgctga tcctcatctc gctcgccggt
     2461 ctcggcttcg gcgccatgtg gatctatccc ggcgccatgg tggcggacat catcgacgag
     2521 gatcggcggc agtacggcga ggggaggtcg gcggtcttct acggcatgta caagacgctc
     2581 gagaagttcg cgcaggccgg cgcgctgatc gtcggtggca tctcgttcgc gctgttcggc
     2641 gacacctcgg cgaacccgct gggcatccag ctgctcatgc cgctggctgc cggcgccatc
     2701 ttcctcggct tcgtggcggt gtggatcggc tatcacgtcc gggagtcgcc gggtgcgcgg
     2761 ccgggactga ccgaggcggc ggtcgcagcc ggctagggcc ggacgacgta ccgctcgacg
     2821 acctcggcca ggaccggcaa cccgaaggcg atggcgctga ccggcacccg ctcgtcgtgg
     2881 ccgtggaaga gggacaggaa gggcacgtcg ggcgcgagct ggaggggcgc gaagccgtag
     2941 cacgggatgc cgagggtggc cagcgccttc gcatcggtgc ctggcgtgat catcatcggc
     3001 accgacgtgg cgtccgggtc ggcacggcgc agcgcgtcgt gcatgagcgt cacgatctcg
     3061 gcgtcgggat cgcactcgac cgatggaagc tggaccaccg gctccacggt cgcgaggtcg
     3121 ccgacgacct cctggaggtg acgcaggaag gcgtcctggt ccgtgcccgg gagcgtgcgc
     3181 acgtcgacct ccgcctcacc ggagctgggg atgacgttga ccttcttccc ggcgcgcatg
     3241 acgttcggcg tgacggtgtc gcgcagcatg gcctcgatcg accggcgcag gaccgggtcc
     3301 tcgacgagcg cctccagccg gaccgatgcg ccagccgggt cgtcctcgac catggccgcc
     3361 acctcgtcca ggcccagcct cgacaggaac gcgccgacga cggggtggac gcgcgccggc
     3421 cgctgccgct ggtcctcgac cagggcggtc accgcggccg cgagcctggt ggcggcgttg
     3481 tccgggtgtg gcatggaccc gtggcccggc gtgccggtcg accgcagccg cgtccaggcg
     3541 atgcccttct ccgcaacctg gacggtgtag aaccgacgac ccccgatcgt catcgagtag
     3601 ccgccgacct cgttgatcgc ggcagcggcc gggcgaccct gggcatcggc gaagaggtca
     3661 ggacgctgct cgaccagggc gcgggcgccg tgctcgccgc ccgcctcctc gtcggccacc
     3721 gtggcgaaga tcacgtcacg ccgcagcggg acgccggagc gggccagggc gagcatcacg
     3781 ccgagctcca tggccaccat gttcttcatg tcgaccgcgc cgcgccccca caccacgccg
     3841 tccaccagct ccgcgccgaa cgggtcgcgg gtccaggcct cggcgtcgac cggcaccacg
     3901 tcgatgtggc tgagcaggat cagcggcggt tcgcttgccg gtccctccgc gcgcaggcgc
     3961 gccacgcagg agccacggcc gggcgtggtg ggctccagca cctccgcctc gatgccggcg
     4021 tcgcgcagca cgtccgcgca gtaggtggcg gccgccgtct cgccgccggc cgggtctcgt
     4081 ccggccgcga cgtcgtcacc cccggtcggc gggttgacgc tcgggatgcg gatcaggtcg
     4141 cgcaggtgcg tgatgcaggc gtcgaggtcc aggtgcggcc gaagggcgtc ggtcatgcgg
     4201 gctacggtag cggctcgctc gccgttcgca cggtcgcctc tcgcggggcg ctcctggcgg
     4261 acgccgccgc cgtcagccct cgccggtgcg ccgcgatccc gagcggcagc tcgagcgcca
     4321 gcatggcgag ggcgaccaac agcagcaccg gctcgctcac cgcgaggccg agccacacca
     4381 gggcggccag gaagaccagg tggatcgcga ggcggctggc gaaggccagg cgcaggtcca
     4441 ggatcgcatc ggcggcgccg tcggcgcgtc cgcggtcggg accgaggacg gcccacaccg
     4501 agatcgcgac gaaggcggcc agcaccaccc agaagaccgc cagcccgccg ccgcgggtcg
     4561 gatcggtcat cggcttgacg atcgcgaagg cgccgatgct ggcgatcagc acgccgaagg
     4621 ctgggattgg caccagcagc tggagccagt cgagcatcgg tcgggtgtgc gctcgcccgc
     4681 gcagccagcg cgtgaagagg ctgaaggcga gggtgacgaa cagccacgag tagaagttgc
     4741 cggccggcac gccgaaccag ccctgctcg
//
LOCUS       DASTYP010000006         5546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1239260, whole genome shotgun sequence.
ACCESSION   DASTYP010000006 DASTYP010000000
VERSION     DASTYP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239260"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFV59_00310"
     CDS             <1..620
                     /locus_tag="VFV59_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5324307.1"
                     /translation="KGITALQMDIKVRGITSEIMQDALAQAREGRMFIMGKMLAAIER
                     PRTELSAYAPRITKISINPDRIRDVIGPGGKMIRKIVDETKCTVDVSDDGTVLIGSTN
                     ADYAKKAIDWIEKLTKDVEAGTIYTGKVTRLMAFGAFVEILPGKEGLVHISQLADYRV
                     PRVEDVVSIGDELMVVVTEIDRMGRVNLSRRAAMERHMAKAGGNS"
     gene            637..1326
                     /locus_tag="VFV59_00315"
     CDS             637..1326
                     /locus_tag="VFV59_00315"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5324308.1"
                     /translation="MEGAGGSVGGAAALAQVAAEVRGCTRCPLSARRTRAVPGEGNVL
                     SDVLLVGEGPGAREDSTGRPFVGPAGQLLTELLRSIGWEREDVFITNVVKCRPPGNRD
                     PEPGEVGACGTYLDRQERALDPAVVVTLGRHSLQRYLPGARISAIHGRLRRSPSGQHV
                     FPMYHPAAALHQASLRETLFRDVRELPAALLVAREALDRERIEAAGAEEAVAPGPPEP
                     PADDAQQMTLF"
     gene            1335..2999
                     /locus_tag="VFV59_00320"
     CDS             1335..2999
                     /locus_tag="VFV59_00320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5324309.1"
                     /translation="MPDSLRIIPLGGVGEVGKNITVVEAGGEILVVDCGLAFPEPEML
                     GIDLVIPDVTYLEEHRDRVRGIILTHGHEDHTGALPYVLPKIPGTPIWATKLTEGLVQ
                     GKLKEHKLLDSTPLHVVEPGREFAVGPFRITPFRVNHSIPDGVGYAIDTPLGTVVHTG
                     DFKFDHTPPDGQRADLGLIAEIGNRGVQFLLSDSTRAEKEGYTLSEATVGDSLHRLVG
                     EAPGRVIVATFASNIGRVQQVIDAAAAHGRKMVALGRSMEQNIAIALERGYLDDRDGT
                     LVKKDQLQRLNHAELVVMTTGSQGEPMSGLTRMSNRDHRNITIEPGDTVIVSASPIPG
                     NEEAVAKTIDNLFKEGAMVHYEPLVHCHVSGHGSREELKLMLGLVKPRHFIPVHGEYR
                     MLVQHGLLAEGAGVDVDKIFVLENGQVVEFDGQKARMSSTVSAGAVLVDGIAAIDGID
                     GVVLRDRRMLASDGVVMVALTVDAKTGQVLGEPDIVSRGFLSDPDDPVIGEAREHLVS
                     ALRQPRHDEHTAEPGYLKTKVRDTLSRYFHQRTKRRPMILPIVMEV"
     gene            3006..5435
                     /locus_tag="VFV59_00325"
     CDS             3006..5435
                     /locus_tag="VFV59_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU5324310.1"
                     /translation="MATRRRTTTRRRTTRRRRQQTPFVVSGRFLREGLALVLLFLAII
                     SVIALFAPNAGAIVQPWHDLLTTLLGWGIAFAAPLLAGFAVMLWMKTMPAERWMAATG
                     AALVALALLGMFHLAAGGTAELVGSGEGGGAIGFAVAGLLKGAVGDAGAWIMLVILAV
                     VGLLLYFNMTIGDLVAAYLAERDERREMEEEAARKAAGRRRPADAAGRSPEPAAAPEA
                     RPGLLDRVRDRLAGGHDAEDEPPLIVRRERPAPATNGGARPANAAIPPPAPMPMASIG
                     TSGLADGETPADEARAGTGTDDLEHGEEARLAAAAREEAADAALESTQRSWDLPGLEL
                     LADAPESSAAQMDLTAKGQRIRETLAHFGIGVKVARIQEGPVVTQYALEVEPGIKLSR
                     IEGLSDNLALALSARSIRIEAPIPGEPYVGVEIPNSAFDLVTLKEVLASRNFAEASDR
                     SSLAFALGQDVAGQPFSADLSKMPHLLIAGATGSGKSVCVNAIIGSFLMNTTPAEVKL
                     ILIDPKRVELAQYKGIPHLLTEVIVEPDKAVNALKWTVGTMESRYAEFAARGVRNIAG
                     YNEALRPGEPRMPYIVVVIDELADLMMISAYEVEATITRIAQLARATGIHLVVATQRP
                     SVQVITGLIKANIPSRIAFAMTSGIDSRTILDTTGAEDLLGRGDMLYQPIDAPRAVRL
                     QGVLVTDPEIDAVARHWRAQGGPQYRPEITAPKRDGKAGGRPGEDEEEDDADPLLTQA
                     VDIVRRSDKASASLLQRRLRIGYARAARILDQMEDRGIVGPADGSRFREVLVTPDGWG
                     GDIAPDEDDMS"
BASE COUNT          886 a         1970 c         1952 g          738 t
ORIGIN      
        1 agaagggcat caccgcgctc cagatggaca tcaaggtgcg cgggatcacc agcgagatca
       61 tgcaggacgc cctggcgcaa gcgcgcgagg gccggatgtt catcatgggc aagatgctcg
      121 cggcgatcga gcgcccgcgc accgagcttt cggcgtacgc tccgcgcatc accaagatct
      181 ccatcaaccc ggaccgcatc cgcgacgtca tcggccctgg tggcaagatg atccgcaaga
      241 tcgtggacga gacgaagtgc accgtggacg tctcggacga cggcacggtc ctgatcggct
      301 cgaccaacgc cgactacgcg aagaaggcga tcgactggat cgagaagctg accaaggacg
      361 tcgaggccgg caccatctac accggcaagg tgacccggct gatggcgttc ggcgccttcg
      421 tcgagatcct gcccggcaag gagggcctgg tccacatcag ccagctcgcc gactaccggg
      481 tgccgcgggt cgaggacgtg gtcagcatcg gcgacgagct gatggtcgtg gtcacggaga
      541 tcgaccgcat gggtcgcgtc aacctgagcc gccgcgcggc gatggagcgg cacatggcga
      601 aggcgggcgg caacagctga gcccggcccg cttgcgatgg agggggcggg cggatcggtc
      661 ggaggcgcgg cggcgctggc gcaggtcgcg gctgaggtcc gcggctgcac ccgctgtccg
      721 ctctccgcgc gccgtacccg cgccgtcccg ggagaaggca acgtcctgag cgacgtgctc
      781 ctggtggggg agggacccgg tgcccgggaa gattccaccg gccgcccgtt cgtcgggccg
      841 gccggccagc tgctcaccga gctgctgcgc agcatcggct gggagcggga ggacgtcttc
      901 atcaccaacg tggtgaagtg ccgtccgccc ggcaaccgcg acccggagcc cggcgaggtg
      961 ggggcctgtg gcacctacct ggatcgacag gagcgagcgc tcgatccggc cgtggtcgtg
     1021 acgctcggcc ggcactccct ccagcgctac ctgccgggtg cccgcatcag cgccatccac
     1081 ggccggcttc gtcggtcacc ctcgggccag cacgtcttcc cgatgtacca tccggctgcg
     1141 gcactgcacc aggcctcgct gcgcgaaacg ctcttccggg acgtgcgcga gctgcccgcg
     1201 gcgctgctgg tcgctcgcga ggcgctcgac cgtgagcgaa tcgaggcggc tggggccgag
     1261 gaggcggtgg cgccgggacc accggagcca ccggccgacg acgcccaaca gatgacactc
     1321 ttctaggagc ttccatgccc gattccctgc gcatcatccc gctcggcggg gtgggcgagg
     1381 tcggcaagaa catcaccgtg gtcgaggccg gcggcgagat tctcgtcgtc gactgcggcc
     1441 tcgccttccc cgagcccgag atgctgggca tcgacctcgt gatccccgac gtgacctacc
     1501 tcgaagagca ccgggaccgc gtgcgcggca tcatcctgac ccacggccac gaggaccaca
     1561 ccggcgccct gccgtacgtt ctgccgaaga tccccggcac gccgatctgg gcgaccaagc
     1621 tcaccgaggg cctggtccag ggcaagctga aggagcacaa gctcctcgac agcacgccgc
     1681 tgcacgtggt cgagccgggg cgcgagttcg ccgtcggccc gttccggatc acgccgttcc
     1741 gcgtcaacca ctccatccct gatggcgttg gctacgccat cgacacgccg ctgggcaccg
     1801 tggtccacac cggcgacttc aaattcgacc acacgccgcc cgacggccag cgcgccgacc
     1861 tgggcctcat cgccgagatc ggcaaccgcg gcgtgcagtt cctgctgtcc gactccacgc
     1921 gggccgagaa ggagggctac accctctcgg aggcgacggt gggggacagc ctgcatcggc
     1981 tggtgggtga ggcgcccggg cgcgtgatcg tcgcgacctt cgccagcaac atcggccgcg
     2041 tccagcaggt gatcgacgcc gccgccgcgc acggccgcaa gatggtggcg ctcggccgga
     2101 gcatggagca gaacatcgcc atcgccctgg agcgtggcta cctcgacgac cgcgacggaa
     2161 ccctggtcaa gaaggaccag ctgcagcgcc tgaaccacgc cgagctggtc gtcatgacga
     2221 ccgggtcgca gggcgagccg atgtcgggcc tgacccggat gagcaaccgc gaccaccgca
     2281 acatcaccat cgaacccggt gacacggtga tcgtgtcggc ctcgccgatc cccggcaacg
     2341 aggaggcggt ggccaagacc atcgacaacc tcttcaagga gggggcgatg gtccactacg
     2401 agccgctggt gcactgccac gtctcgggcc acgggtcccg cgaggagctc aagctcatgc
     2461 tcgggctcgt caagccgcgg cacttcatcc cggtccacgg ggagtaccgc atgctggtcc
     2521 agcacggcct gttggccgag ggagccggcg tcgacgtcga caagatcttc gtcctcgaga
     2581 acggccaggt cgtcgagttc gacggccaga aggcccgcat gtcgagcacg gtcagcgccg
     2641 gggccgtgct cgtggacggc atcgcggcca tcgacgggat cgacggcgtc gtgctgcgcg
     2701 accgacgcat gctcgcctcc gatggcgtgg tgatggtcgc cctcaccgtc gacgccaaga
     2761 ccggccaggt cctgggagag cccgacatcg tcagccgcgg cttcctctcc gatccggacg
     2821 accccgtcat cggcgaggct cgcgagcacc tggtctccgc gctgcggcag ccgaggcacg
     2881 acgagcacac cgccgagccg ggctacctca agaccaaggt gcgcgacacg ctcagccgat
     2941 acttccacca gcgcaccaag cgccggccca tgatcctgcc gatcgtgatg gaggtctgac
     3001 ccggcgtggc cacccgacgg cgcacgacca cccgtcgccg caccacccga cgccggcgtc
     3061 agcagacgcc gttcgtcgtc agcggccgct tcctgcgcga agggctggcg ctcgtcctcc
     3121 tcttcctggc gatcatcagc gtcatcgcgc tgttcgcccc gaacgcggga gcgatcgtcc
     3181 agccctggca cgacctcctg acgaccctgc tcgggtgggg gatcgcgttc gccgccccgc
     3241 tgctcgccgg gttcgcggtc atgctgtgga tgaagaccat gcccgcggag cgctggatgg
     3301 cggccaccgg cgccgcgctg gtggcgctgg cgttgctcgg gatgttccac ctggccgcag
     3361 gcgggaccgc cgagctggtc gggtccggcg aaggtggggg agccatcggc ttcgcggtag
     3421 ccggcctcct caagggcgcg gttggcgacg ccggtgcctg gatcatgctc gtgatcctcg
     3481 cggtcgtcgg gctgttgctc tacttcaaca tgaccatcgg cgacctggtg gcggcctacc
     3541 tcgccgagcg cgacgagcgc cgcgagatgg aggaagaggc cgcgcgcaag gccgctggtc
     3601 gccgccgacc ggcggacgcc gccggtcggt ccccggagcc ggcagcggcg cccgaggcac
     3661 gtcctggcct gctggaccgc gtccgcgacc gcctggccgg cggacacgac gccgaggacg
     3721 agccgccgct gatcgtgcga cgtgagcgtc ccgcaccggc gaccaacggc ggcgcccggc
     3781 cggcgaacgc agccatcccc ccgccggccc cgatgccgat ggcttcgatc ggcacttcgg
     3841 gcctcgcgga tggagagacg ccggcggacg aagcccgggc cggcaccggc accgacgacc
     3901 tggagcacgg cgaggaggca cgcctcgccg cggccgcgcg ggaggaggcg gcggacgcgg
     3961 cgctcgagtc cacccagcga tcatgggacc tgccgggact cgagctgctc gccgacgccc
     4021 ccgagtcgag cgctgctcag atggacctca ccgccaaggg ccagcgcatc cgcgagacgc
     4081 tggcgcactt cggcatcggc gtgaaggtgg cgcgcatcca ggaggggccg gtcgtcacgc
     4141 agtacgccct ggaggtcgag ccgggcatca agctcagccg gatcgagggg ctgtcggaca
     4201 acctggccct ggcgctgtcg gcgcgcagca tccgcatcga ggcgccgatt cccggcgagc
     4261 cgtacgtcgg ggtcgagatt ccgaactcgg cgttcgacct ggtgaccctc aaggaggtgc
     4321 tggcgagccg caacttcgcg gaggcatcgg atcggtcctc gctggccttc gcgctcgggc
     4381 aggacgtggc cggtcagccg ttcagcgccg acctgtcgaa gatgccgcac ctgctcatcg
     4441 cgggcgccac cggatcgggc aagagcgtgt gcgtcaacgc catcatcggc agcttcctga
     4501 tgaacacgac gccggcggag gtgaagctca tcctcatcga ccccaagcgg gtcgagctgg
     4561 cgcagtacaa gggcatcccg cacctgctga ccgaggtcat cgtcgagccg gacaaggcgg
     4621 tcaacgccct gaagtggacg gtcggcacca tggagagccg atacgccgag ttcgccgccc
     4681 gcggcgtgcg caacatcgcc ggctacaacg aggcgctgcg tccgggcgag ccgcgcatgc
     4741 cgtacatcgt cgtggtcatc gacgagctcg cggacctgat gatgatcagc gcctacgagg
     4801 tcgaggcgac catcacgcgc atcgcgcagc tggcccgtgc caccggcatc cacctggtcg
     4861 tcgcgacgca gcgcccgagc gtgcaggtca tcaccgggct catcaaggcc aacatcccgt
     4921 cgcgcatcgc gttcgccatg acctccggca tcgactcgcg caccatcctg gacaccacgg
     4981 gggcggagga tctgctgggc cgcggcgaca tgctctacca gccgatcgac gcgccccgcg
     5041 ccgtccgcct gcagggcgtg ctggtcaccg acccggagat cgacgcggtc gcgcgccact
     5101 ggcgcgccca gggcggcccg cagtaccgcc cggagatcac cgcgccaaag cgcgacggga
     5161 aggccggcgg gcggccgggc gaagacgagg aggaggacga cgccgatccg ctgctgacgc
     5221 aggcggtcga catcgtgcgg cgctcggaca aggcgtcggc gtccctgctg cagcggcgcc
     5281 tgcgcatcgg ctacgcgcgc gcggcgcgca tcctggacca gatggaggac cgcggcatcg
     5341 tgggacccgc ggacgggagc cggttccgcg aggtcctggt cacgccggac ggctggggcg
     5401 gcgatatcgc gcccgacgag gacgacatgt cgtgagcgac cgggtccacc agctcgggga
     5461 ggtgctgcgc accgcccgcg aggcgaaggg cgtggacctg gcccgggtcg agcgagacac
     5521 gaagatccgg gagcggtacc tctcgg
//
LOCUS       DASTYP010000007        13570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1093581, whole genome shotgun sequence.
ACCESSION   DASTYP010000007 DASTYP010000000
VERSION     DASTYP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093581"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VFV59_00330"
     CDS             complement(<1..1176)
                     /locus_tag="VFV59_00330"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324311.1"
                     /translation="MTAPISASWLGTQRWFRAKQRPIAAVHEADRAPIRDRAALVVAE
                     VGYVDGGQADRYLLVEVDGGEPADGDGAWRSLVRAMAQADELSAARGAFACAPTRALE
                     GLLPASAESAATLTERRLRVEQSNTSVVLGDRLILKLFRLLEPGDNPDLEISAFLADA
                     GFTDTPALAGSITYRPDGAPPAAAAMLQAFVPSRGDAWAAMLEALATEPGRGIAMAAE
                     IADLTRRMHGALASRPDDPDFPARTATVDETSSWRASAERQLAQAVTAVGGADHDRLV
                     GLAPAVRARFADTFGRATGEATVSRIHGDYHLGQLLAREGGGFSVIDFEGEPARPLEE
                     RRHPSSPLRDVAGLLRSLDYAARTAADGRHASGFDADAWLAEARAAFLEAYGEIGPED
                     "
     gene            complement(1229..1786)
                     /locus_tag="VFV59_00335"
     CDS             complement(1229..1786)
                     /locus_tag="VFV59_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5324312.1"
                     /translation="MATSEGPMVLRAATAADAATLGDISARAWEATYRGMIPDAELDE
                     WIATAPDAWREGLAKQAAGSASRAWVAERAGSAIGFATTAPGSDRWLPPPEGAGELTN
                     LYLDPDVIGQGVGRALYDHAVGDLAARGFDPLVVWAFRDNHRARRFYEGRGLVMDVAE
                     HAWVLAGVACPIVRFRGDLAPVARG"
     gene            complement(1773..3419)
                     /gene="treS"
                     /locus_tag="VFV59_00340"
     CDS             complement(1773..3419)
                     /gene="treS"
                     /locus_tag="VFV59_00340"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEU5324313.1"
                     /translation="MRDDPHWYKDAIIYQVHVKSFFDSTGDGMGDFAGLTQKLDYIAD
                     LGVTAVWLLPFYPSPLKDDGYDIAEYTRVHPDYGTLRDFRSFVREAHRRGLRVITELV
                     VNHTSDQHPWFVESRSSRRNPKRDWYVWSDTDQTYADARIIFTDTETSNWTWDRESQQ
                     YYWHRFFSHQPDLNYDNPQVVRAVLRVLRFWLDLGVDGLRLDAVPYLVEREGTSCENL
                     PETHAILQRLRAEVDARYTGRMLLAEANQWPPDVLPYFGTPERPECHMAFHFPLMPRI
                     WIALRREDRYPIVEIMGQTPEIPDENQWALFLRNHDELTLEMVTDEERDFMYTEYARD
                     ARMRINVGIRRRLAPLVDNSRRRLELLNSLLLSMKGTPIVYYGDEIGMGDNVYLGDRN
                     GVRTPMQWTGDRNAGFSTADVHALYAPLVADPVYGFQAVNVEAQQRIPGSLLNWMKRI
                     IRVRKQYPVFGRGTLEFLHPENRHVLAYLREHEGVTILCVANLSRFAQYVELDLRRYD
                     GRQPVELIGRVAFPRIGELPYLLTLGPHDFLWFELTDGDV"
     gene            complement(3535..5616)
                     /locus_tag="VFV59_00345"
     CDS             complement(3535..5616)
                     /locus_tag="VFV59_00345"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEU5324314.1"
                     /translation="MTTTKQGLRRAVAAALEHAEPGPGAVGTRRTIIIERIAPELDGG
                     RYPVKRVVGDQLLVTADIFADGHDLLDAALLLRAEDDERWREAPMRPIDNDRWSGHLE
                     LTRNAWHRYAIEAWRDVFGTWRHGLDKKVEAGVAVPSELEEGRLLLEAAIARAERAET
                     ADDVRLLRDALTAFRRARAEATRVAALSGSDLLAAMRRHPDRRFATRSPELPLVVDRE
                     RARFGAWYELFPRSQGRDPKRPTPTTLAEATWRLADIAAMGFDVVYVPPVHPIGRTNR
                     KGKNNTLDATDADVGSPYAIGSVAGGHDAVAPELGGTNAFEAFRREVERHGMELAIDL
                     AIQASPDHPYVTQHPEWFRHSPDGSIKYAENPPKKYQDIYPIFFEGEDHAAREALWHE
                     WKRVFGVWIDRGVRIFRVDNPHTKPIPFWAWLIREIQRDHPEVIFLSEAFTKPKMMRQ
                     LAKVGFTQSYTYFTWRETAADLRDYLTELTRSEMREYYRGNLFTNTPDINPHHLDHGR
                     PAFVARTVLAATLSSSYGIYSGWELTEAARLGEREEYLDSEKYEIRARDWNAPGNIKL
                     LIGELNRLRHERRALQLYDNLRFEPVTAERSLFYRKALPHGKPDPLTGEPQRWRDPVY
                     VAVNCDPQVGERAILHPDLPAVGIGWDEPYRLTDLMTGRTTQERGADLQVDLDPAIES
                     FRIFTIAPLER"
     gene            5914..6957
                     /locus_tag="VFV59_00350"
     CDS             5914..6957
                     /locus_tag="VFV59_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5324315.1"
                     /translation="MRDPIGIAPAPDGWLLVQERAGRLLAVPPGGGTPRVAADLTDRV
                     LGEGERGLLGVALNPGFPDDPRAFVHYTDRDGNTVVSTFSAIQDADGAPLLDVASEQR
                     LLSLQQPYANHNGGQLAFGPDGMLYLGLGDGGSGGDPLGNGQDPSTLLGSILRLDVGG
                     PPQGQAGQPYDIPDDNPFVGGGGAPEVFAFGLRNPWRFSFDRVTGELWIADVGQNAYE
                     EVNRLDLAAAAGANFGWNVMEATHCFAAEDCSSDGLVLPLVEYSHDLGCSVVGGYVYR
                     GSAIEGLAGWYLFSDYCSGTLFGIASDAAGSGLAPRALLETGGSVTSFGEASDGELYL
                     ADIGGTVYRLVGG"
     gene            complement(6936..7631)
                     /gene="radC"
                     /locus_tag="VFV59_00355"
     CDS             complement(6936..7631)
                     /gene="radC"
                     /locus_tag="VFV59_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU5324316.1"
                     /translation="MNTDPIPLRPARSRAAGSVLERLGPEHCEAEELVAAILGDGAGA
                     RRTARRLLDRFGSLRALSRAGLVELRVAGLSEVAAGRLVAAAEVGRRAIREWPAGEWV
                     VRMPADAAEPLVDAMGPLEREELRVLLLDTKNVVVGERTVYRGNLAGSSVRVGEVFRD
                     AVRSCAAAVVVVHNHPSGDPSPSGEDLRITTELAEAGRLLDIELLDHLIIGHGRWASL
                     RALGAVGQPPTSR"
     gene            complement(7690..8631)
                     /locus_tag="VFV59_00360"
     CDS             complement(7690..8631)
                     /locus_tag="VFV59_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU5324317.1"
                     /translation="MLGPNTVRMPSWPGPVLAIGGAILAVGAASARLMVASGQLDADL
                     RLLGWLALAGAVAFVAGLVYAATRQLRVRRYLPPERYRGPAVLLLLALVVVVATIFQI
                     PFGNDILALTGEGGELTPLGAAVVLLSTPVALLLVSWWFVLRPRALAGLPSWMGPDPV
                     RALGIGVLAGIGMWVLGNLVGAAVIALLQALGIGVDTQLAERAVETLDPWLVVLVVIF
                     LAPVAEELFFRGVVFNAWLREGGVRWAFIGSTLLFALIHLSIAAFVPILLLGFALAWI
                     YRRTQSLLAPIALHATFNGISVVLVLLTRFDILKPPV"
     gene            complement(8708..9310)
                     /locus_tag="VFV59_00365"
     CDS             complement(8708..9310)
                     /locus_tag="VFV59_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5324318.1"
                     /translation="MDAILEPIGEFWNGFLSNPIVQLASQLIVIYVVLLWLGTAYWAF
                     RDMQARTENPILPYFAAALIIFFTPLLFVFAVVLYLIVRPRETLAEVYERSLAEESLL
                     AEVEKNELCPVCRDRVDADWLVCPNCRTRLHRVCPSCNRLADPTWPLCAYCGREFERP
                     TRAPRRAAAARPAEEPVTLATDEATAEHTPLRRATNTTNT"
     gene            complement(9370..9960)
                     /gene="purN"
                     /locus_tag="VFV59_00370"
     CDS             complement(9370..9960)
                     /gene="purN"
                     /locus_tag="VFV59_00370"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU5324319.1"
                     /translation="MRLGVLVSGRGSNLEAVLTARIADLEPVLVISNRPGVRALEVAA
                     AHGVPALVLRRRDFADAAARDAEVGRALADAGVDLALLAGYDQLLQPSYFAAFGGSTI
                     NIHPSLLPAHGGAGMMGMAVHRSVLACGDRETGVTIHHVTPELDGGPVIAQAPVPVLP
                     GDDADTLAARVLAEEHRLLVTTLATLAAEAPRHVAC"
     gene            complement(9957..10973)
                     /gene="purM"
                     /locus_tag="VFV59_00375"
     CDS             complement(9957..10973)
                     /gene="purM"
                     /locus_tag="VFV59_00375"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU5324320.1"
                     /translation="MARTYRQAGVDVAEAERAVERIARAAASTATPAVLAGVGGFAGL
                     FRFNPSTHPDPVLVSSTDGVGTKLKVALGLGRHDTIGVDLVNCCLNDILVTGADPLFF
                     LDYLALGDLRADVVESIVGGVAAGCAAAGIPLIGGETAQMPDVYRDDDYDLAGFIVGV
                     VGRSELIDGSRVRAGDVVLGLPSSGLHTNGYTLARRVLPETVWRDPMPHGGGTIGDAL
                     LEPHRSYLDEVRALRRAVDVRAMAHITGGGWEGNIPRTLPEGLGAEIDTGSWPVPPIF
                     NLLQSRGDIDDTEMVRTFNVGIGMTVVVAPDHAGAALDAVPEARRIGRVVDVIGGEPR
                     VRFA"
     gene            complement(10977..12476)
                     /gene="purF"
                     /locus_tag="VFV59_00380"
     CDS             complement(10977..12476)
                     /gene="purF"
                     /locus_tag="VFV59_00380"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5324321.1"
                     /translation="MDPSIVPPLISIDELPPTYAPHGPREECGVVGAWSPGAPVARLA
                     FLGLHALQHRGQESAGIAVSDGEHLSLHKRLGLVAGVFDAETLLRLEDGGQGRPPVAA
                     IGHTRYSTTGSNTLPNVQPVYAQSDRGELMLGHNGNLTNARWLRDELSEQGIDFASTS
                     DTEVLARLIAHAPGRTWGQRVDHAFHRATGAFTFTMLTADALVAGRDPVGFRPLALGR
                     LPNGGWAIASESTALDAMGAQLVRDIEPGEVVTIDAEGVHSHRSSLATPQRERLCVFE
                     YVYLARPDSLIGGRLLYEARLEMGRVLAREQPAEGADLVIAVPDSAIPAAIGYAEESG
                     LPYREGLIKSRYVGRTFIQPAQDGREEAVRLKFNPLPELLSGKRVVVVDDSIVRGTTT
                     GPIVNLLRRAGAAEVHVRIHSPEMRHPCYMGVDTGRRGELIAATHSTEAIRRVIGADS
                     LGFLSREGLLAAVRGANGAGEGGHCTACFDGRYPTAVPMELDKLAMERR"
     gene            12711..>13570
                     /locus_tag="VFV59_00385"
     CDS             12711..>13570
                     /locus_tag="VFV59_00385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5324322.1"
                     /translation="MLTFVRALGFLAPFAIAAVLTIGLGIAAQTEPDQRFKLVAVLVT
                     AAAAGMTTMALLAWSYVGWRLNRIARALEGTLENDEPVELKVSGIPAERRLARAFNAT
                     AGAFFQTEARATHDRLTGIPNRETLLATLNVEIERAARHYKPLSVAFIDIDRFKPIND
                     TYGHKSGDAVLRQVATIIADAIRISDTFGRYGGEEFMLILPETTPDDAVGLAEELRAL
                     VMQEPLRVADGQTIQVTISAGIAGGRGSELQLDMLVDRADAAMYAAKSLGRNRTYQFR
                     DVDEEAPVRRA"
BASE COUNT         1936 a         4834 c         4947 g         1853 t
ORIGIN      
        1 atcctccgga ccgatctcgc cgtacgcctc caggaacgcc gcacgcgcct ccgcgagcca
       61 ggcatccgcg tcgaagcccg acgcgtggcg gccgtcggcg gcggtgcgtg ccgcgtagtc
      121 gaggctgcgc aggaggccgg ccacgtcgcg cagcggcgat gacgggtggc ggcgctcctc
      181 gagcggacgc gccggctcgc cctcgaagtc gatcacgctg aagccgccgc cctcgcgggc
      241 gagcagctgt cccaggtggt agtcaccgtg gatccggctg acggtcgcct cgccggtggc
      301 gcggccgaag gtgtccgcga atcgggcgcg cacggcaggg gcgaggccga ccagccggtc
      361 gtgatcggca ccgccgaccg ccgtgacggc ctgtgccagc tgccgctcgg ccgacgcgcg
      421 ccagctcgac gtctcgtcga cggtggcggt ccgcgccggg aagtccgggt cgtcgggtcg
      481 gctggcgagg gccccgtgca tccggcgcgt caggtccgcg atctcggccg ccatggcgat
      541 cccgcgtccg ggttccgtgg cgagggcctc gagcatcgcc gcccacgcgt cgccgcgcga
      601 gggcacgaag gcctggagca tcgcggccgc ggcgggcggt gcgccatcgg ggcggtaggt
      661 gatgctgccg gccagggccg gcgtatcggt gaagccggca tcggccagga aggcgctgat
      721 ctcgaggtcc ggattgtctc cgggctcgag caggcggaac agcttgagga tcagccggtc
      781 gccgagcacg accgaggtgt tcgactgctc gacgcgaagg cgccgctcgg tgagggtcgc
      841 cgccgattcc gccgatgccg gcaggagccc ctcgagcgca cgcgtcgggg cgcaggcaaa
      901 ggcgccgcgc gccgcactca gctcgtcggc ctgggccatg gcccggacca gcgagcgcca
      961 ggcaccgtca ccgtccgcgg gctcgccgcc gtccacctcc accagcaggt agcggtccgc
     1021 ctggccaccg tcgacgtacc cgacctccgc cacgaccagc gcggcgcggt cgcgaatcgg
     1081 cgcgcggtcg gcctcgtgga ccgcggcgat cgggcgctgc ttggcgcgga accagcgctg
     1141 ggtccccagc caggacgccg agatcggtgc cgtcacccgg cgcgccaggt acgccggggt
     1201 cgcgatggcc ggcgcggcgg aaccctcgtc atcctcgggc caccggcgcc aggtcgccgc
     1261 ggaagcggac gatcgggcac gcgacgccgg cgagcaccca cgcgtgctcg gccacgtcca
     1321 tcaccaggcc gcgaccctcg tagaagcgac gggctcggtg gttgtcacgg aacgcccaga
     1381 ccaccagcgg atcgaaccca cgcgcggcga ggtcccccac ggcgtggtcg tacagcgcgc
     1441 gtccgacgcc ctgcccgatg acgtcaggat cgaggtacag gttcgtcagc tcgccggcgc
     1501 cctccggcgg cggcagccac cgatcgctcc cgggggcggt ggtcgcgaag ccgatcgcgc
     1561 tccccgcccg ctccgcgacc cacgcccggg acgcggaacc cgccgcctgc ttcgccagtc
     1621 cctcgcgcca ggcatcggga gcggtcgcga tccactcgtc cagctccgcg tcgggaatca
     1681 tgccgcggta ggtcgcctcc caggcccggg cgctgatgtc gccgagcgtg gccgcatcgg
     1741 cggccgtcgc ggcgcggagg accatcggcc cctcagacgt cgccatcggt cagctcgaac
     1801 cagaggaagt cgtgcgggcc gagggtcagc aggtagggca gctccccgat gcgcgggaag
     1861 gcgacccgtc cgatcagctc gaccggctgc cgcccgtcgt agcggcgcag gtccagctcc
     1921 acgtactggg cgaaccgcga gaggttcgcg acgcacagga tggtgacgcc ctcgtgctcg
     1981 cgcaggtagg cgaggacgtg gcggttctcg gggtgcagga actcgagcgt gccgcggccg
     2041 aagaccgggt actgcttgcg cacgcggatg atccgcttca tccagttgag gagcgagccc
     2101 gggatgcgct gctgcgcctc gacgttgacc gcctggaagc cgtagaccgg atcggccacc
     2161 agcggcgcgt agagggcgtg gacgtcggcg gtgctgaagc cggcgttgcg gtcgccggtc
     2221 cactgcatcg gtgtgcgaac gccgttccgg tcgcccaggt agacgttgtc gcccatgccg
     2281 atctcgtcgc cgtagtacac gatcggcgtg cccttcatgc tcagcagcag cgagttgagc
     2341 agctcgaggc ggcggcgcga gttgtcgacc agcggggcca ggcgccgtcg gatgccgacg
     2401 ttgatgcgca tccgggcgtc ccgcgcgtac tcggtgtaca tgaagtcgcg ctcctcgtcg
     2461 gtgaccatct cgagcgtcag ctcgtcgtgg ttgcgcagga agagcgccca ctggttctcg
     2521 tccgggatct cgggcgtctg gcccatgatc tcgacgatcg ggtagcggtc ctcgcgtcgc
     2581 agcgcgatcc agatgcgcgg catcagcggg aagtggaacg ccatgtggca ctcgggccgc
     2641 tccggcgtcc cgaagtaggg cagcacgtcg ggtggccact ggttcgcctc ggccagcagc
     2701 atgcgcccgg tgtagcgggc gtcgacctcg gcccgcagcc gttgcaggat ggcgtgcgtc
     2761 tcgggcaggt tctcgcagct cgtaccctcg cgctcgacca ggtacggcac ggcgtccagg
     2821 cgcagcccgt cgacgcccag gtccagccag aagcgcagca cgcggagcac ggcgcgcacg
     2881 acctgcgggt tgtcgtagtt gagatcgggc tggtggctga agaaccgatg ccagtagtac
     2941 tgctgcgact cgcggtccca cgtccagttg ctggtctcgg tgtcggtgaa gatgatccgc
     3001 gcgtcggcgt acgtctggtc ggtgtcgctc cacacgtacc agtcgcgctt cgggttgcgg
     3061 cggctggacc gcgactccac gaaccacggg tgctggtcgc tggtgtggtt gacgaccagc
     3121 tcggtgatga cgcgcaggcc tcgccgatgc gcctcgcgga cgaagctgcg gaagtcgcgc
     3181 agcgtcccgt agtcggggtg gacgcgggtg tactcggcga tgtcgtagcc gtcgtccttg
     3241 aggggcgagg ggtagaacgg gagcagccag acggcggtga ctccgaggtc ggcgatgtag
     3301 tccagcttct gggtcaggcc ggcgaagtcg cccatgccgt cgccggtgga gtcgaagaac
     3361 gacttcacgt ggacctggta gatgatcgcg tccttgtacc agtgcgggtc gtcgcgcagg
     3421 agctggggcg cgggcgcgtc cgcgcggtcg acgacgaggc cccccggctc cgacgacggt
     3481 gctctggtca cgtggtctcc cacctccgat gcagcggtgc cgccgccggt cggctcagcg
     3541 ctccagcggc gcaatcgtga agatccggaa cgactcgatc gccgggtcga ggtcgacctg
     3601 gaggtccgcg ccgcgctcct gcgtggtacg gccggtcatc aggtcggtga ggcggtacgg
     3661 ctcgtcccag ccgatgccga cggcgggcag atcggggtgc aggatcgcac gttcgccgac
     3721 ctgcgggtcg cagttgaccg ccacgtacac cgggtcgcgc caccgttgcg gctccccggt
     3781 cagcggatcg ggctttccgt gcggcagggc cttgcgatag aagaggctgc gctcggcggt
     3841 caccggctcg aagcgcaggt tgtcgtagag ctgcagggcg cgacgctcgt gtcgcaggcg
     3901 gttcagctcc ccgatcagca gcttgatgtt gcccggcgcg ttccagtcgc gagctcggat
     3961 ctcgtacttc tcgctgtcga ggtactcctc ccgctcgccg aggcgggcgg cctccgtcag
     4021 ctcccagccc gagtagatcc cgtagctcga gctgagcgtc gccgcgagca cggtgcgggc
     4081 cacgaaggcc ggccggccgt ggtccaggtg gtgcggattg atgtccggcg tgttggtgaa
     4141 caggttcccg cggtagtact cccgcatctc cgaccgcgtc agctcggtga ggtagtcgcg
     4201 aaggtcggcg gccgtctcgc gccaggtgaa gtaggtgtag ctctgggtaa agccgacctt
     4261 cgccagctgc cgcatcatct tcggcttggt gaaggcctcg gagaggaaga tcacctcggg
     4321 gtgatcacgc tggatctccc ggatgagcca cgcccagaac gggatcggct tggtgtgcgg
     4381 gttgtccacc cggaagatgc gcaccccgcg gtcgatccag actccgaaga cccgcttcca
     4441 ctcgtgccac agcgcctcgc gcgccgcgtg gtcctcgccc tcgaagaaga tcgggtagat
     4501 gtcctggtac ttcttcggcg ggttctccgc gtacttgatc gatccgtccg gcgagtggcg
     4561 gaaccactcg gggtgctggg tgacgtacgg gtggtccggg gacgcctgga tggcgaggtc
     4621 gatggccagc tccatgccgt gccgctccac ctctcggcgg aaggcctcga acgcgttggt
     4681 gccgcccagc tcgggcgcga cggcgtcgtg acctcccgcg actgagccga tggcgtaggg
     4741 cgacccgacg tcggcatcgg tcgcgtcgag cgtgttgttc ttccccttgc ggttggttcg
     4801 accgatgggg tggaccggcg ggacgtacac gacgtcgaac cccatggcgg cgatgtccgc
     4861 cagccgccac gtggcctcgg ccagggtggt cggcgtgggc cgcttcgggt cgcgcccctg
     4921 cgaccgcggg aagagctcgt accacgcccc gaagcgcgcc cgctcgcgat cgaccaccag
     4981 cggcagctcg ggcgagcgcg tcgcgaagcg acgatccggg tggcgccgca tggcggcgag
     5041 caggtcgctg cccgagaggg ccgcgacgcg ggtcgcctcg gcacgggcgc ggcggaaggc
     5101 ggtgagggcg tcgcgcagga gccgcacgtc gtcggcggtc tcggctcgct cggccctcgc
     5161 gatggcggcc tccagcagca gccgtccctc ctcgagctcg ctgggcacgg cgacgccggc
     5221 ctcgaccttc ttgtcgagac catggcgcca cgtgccgaag acgtcgcgcc acgcctcgat
     5281 ggcgtagcgg tgccacgcgt tccgcgtcag ctcgaggtgg cccgaccagc ggtcgttgtc
     5341 gatcgggcgc atcggggcct cgcgccaccg ttcgtcgtcc tcggcgcgga gcagcagcgc
     5401 ggcatcgagc aggtcgtggc cgtcggcgaa gatgtcggcg gtgaccagca gctggtcacc
     5461 gaccacccgc ttcaccgggt atcggccgcc gtccagctcc ggtgcgatgc gctcgatgat
     5521 gatcgtccgg cgcgtgccga ccgcccccgg accgggctcg gcgtgctcca gcgccgcggc
     5581 gaccgcccgg cgcaatccct gcttcgtcgt ggtcatggct gaagcgcaac cctacccgat
     5641 cgtttgacaa tggcgcccat gaaccgcgtc gggctgcccg cgctctccat gctctccgtg
     5701 ctcatggccg ccaccctggc tgcatgtggc acgccatccc cgaccccggc gactgaaggc
     5761 tccgcgacgg cttcgggctc accatcgacc ccgacacgtg cggcgacccc ctcggcctcc
     5821 tccacggcgg cggcgtcgcc aagcgtcgct gagaccgttc caccggtcag cgccgaacct
     5881 ccggcgctcg ccctcgaggc cgtcgcgacc ggggtgcgtg accccatcgg gatcgccccc
     5941 gcaccggatg gctggctgct ggtccaggag cgcgccggcc gcctgctcgc cgtgcctccg
     6001 ggcggcggga cgccgcgcgt cgcggcggac ctgaccgacc gcgtgctcgg tgagggcgag
     6061 cgcggactgc tgggcgtcgc gctgaacccc ggcttccccg acgatccgcg cgccttcgtg
     6121 cactacaccg accgcgatgg caacacggtg gtctcgacct tctccgccat tcaggacgcg
     6181 gacggggcgc cgctcctgga tgtcgcctcc gagcagcgcc tgctcagcct ccagcagccg
     6241 tacgcgaacc acaacggtgg acagctggcc ttcggcccgg acggcatgct gtacctcggc
     6301 ctgggggacg gcggctccgg tggcgatccg ctgggcaacg gccaggaccc atcgacgctg
     6361 ctgggctcga tcctgcgcct cgacgtcggc gggccgccgc agggccaggc cgggcagccc
     6421 tacgacatcc cggacgacaa cccgttcgtc ggtggcggcg gagcccccga ggtgttcgcc
     6481 ttcggccttc ggaacccgtg gcgcttcagc ttcgaccgcg tcaccggcga gctgtggatc
     6541 gccgatgtcg gccagaacgc ctacgaggag gtcaaccgcc tggacctcgc ggccgccgca
     6601 ggcgcgaact tcggctggaa cgtcatggag gcgacgcact gcttcgcggc cgaggactgc
     6661 tcctcggacg gcctggtgct gccgctcgtc gagtactcgc acgacctggg ctgctcggtg
     6721 gtcggcggct acgtctaccg cggctcggcc atcgagggcc tggcggggtg gtacctgttc
     6781 agcgactact gcagcgggac gctgttcggc atcgcgtcgg acgcggcggg gagcgggctc
     6841 gctccccgcg cactcctgga aactggcggt tccgtgacga gcttcggcga ggcatccgac
     6901 ggggagctct acctggccga catcggtggg acggtctacc ggctggtggg cggctgaccc
     6961 acggcgccga gtgctcgcag gctggcccac cgtccgtggc cgatgatgag gtggtccagc
     7021 agctcgatgt cgagcagccg tccggcctcg gccagctcgg tggtgatgcg gaggtcctcg
     7081 ccggacggtg aagggtctcc ggacgggtgg ttgtggacga cgacgaccgc ggccgcacag
     7141 ctgcggaccg cgtcgcggaa cacctcgccg acacgaaccg acgaaccggc caggttgccg
     7201 cggtagaccg tccgttcgcc gaccacgacg ttcttcgtgt cgagcagcag cacgcgcagc
     7261 tcctcgcgct cgagcgggcc catggcgtcg accagcggct cggcggcgtc ggccggcatg
     7321 cggacgaccc actccccggc gggccactcc cgaatcgcac gtcggccgac ctcggcggca
     7381 gcgaccaggc ggccggccgc gacctcagag agtcccgcga cgcgcagctc caccaggccg
     7441 gcgcgcgaca gcgcgcgcag cgatccgaat cggtccagca gccggcgggc cgtccgccgt
     7501 gcgccggcac cgtcacccag gatggcggcc accagctcct cggcctcgca gtgctccgga
     7561 ccgagccgct ccagcacgga gccggcggcg cgtgaccgcg ccggtcgcag gggaatcgga
     7621 tcggtgttca tcggcctcga cgatacggat cgacgatgac cgaccgctta ccgccggctt
     7681 accgcgggcc tagaccggtg gcttgaggat gtcgaaccgc gtcagcagca cgaggacgac
     7741 cgagatgccg ttgaaggtcg cgtgcagcgc gatcggcgcc agcaggctct gcgtgcgtcg
     7801 atagatccac gccagcgcga agccgagcag gaggatcggc acgaaggccg cgatcgagag
     7861 gtggatcagg gcgaagagca gcgttgagcc gatgaaggcc cagcggacgc cgccctcccg
     7921 cagccaggcg ttgaacacga cgccgcggaa gaacagctcc tccgcgaccg gcgccaggaa
     7981 gatgaccacg agcacgacca gccacggatc gagcgtctcg acggcgcgtt cggcgagctg
     8041 cgtatcaacc ccaatgccca aggcctgcag cagggcaatg accgccgcgc cgaccaggtt
     8101 gccgaggacc cacatgccga tgccggccag cacgccgatg cccagggcgc gcaccgggtc
     8161 ggggcccatc caggagggca gcccggccag cgcgcgcggg cggagcacga accaccagct
     8221 gaccagcagc agcgccacgg gggtggagag cagcacgacg gccgcgccca gtggcgtgag
     8281 ctcgccgccc tcgccggtca gggccaggat gtcgttgccg aacgggatct ggaagatcgt
     8341 ggcgacgacg accacgaggg cgagcagcag cagcacggcc gggccgcggt agcgctcggg
     8401 cggcaggtag cgccggacgc ggagctgtcg cgtggccgcg tagaccaggc cggccacgaa
     8461 ggcgacggct ccggcgaggg cgagccagcc gagcaggcgc aggtccgcgt ccagctggcc
     8521 cgaggcgacc atgaggcgcg cactcgccgc gccgaccgcc aggatcgcgc cgccgatggc
     8581 gagcaccggt cccggccacg agggcatgcg gaccgtgttc gggccgagca cggggtgggg
     8641 tccggtggga agcgtgccgc gaccagcggc tggatccggc cccacgaacg gcggctcgct
     8701 cagccgctca ggtgttggtc gtgttcgtcg cgcgccgcag cggggtgtgc tcggcggtgg
     8761 cctcgtcggt ggccagcgtc acgggctcct cggcgggacg agccgcggcg gcccgtcgcg
     8821 gggcacgagt cggtcgctcg aactcgcggc cgcagtaggc gcacagcggc caggtcggat
     8881 cggccaggcg gttgcacgag ggacagacgc ggtgcagccg ggtccggcaa ttcgggcaga
     8941 cgagccagtc ggcatccacg cggtcacggc acaccggaca gagctcgttc ttctcgacct
     9001 cggccagcag gctctcctcg gcgagggagc gctcgtacac ctcggccagc gtctcgcgcg
     9061 gccgcacgat caggtacagc accaccgcga agacgaacag gagcggcgtg aagaagatga
     9121 tcagcgccgc cgcgaagtac ggcaggatcg ggttctcggt gcgcgcctgc atgtcgcgga
     9181 aggcccagta ggcggtgccg agccacagga gcacgacgta gatcacgatc agctggctgg
     9241 ccagctgcac gatcgggttg ctcaggaagc cgttccagaa ctcgccgatc ggttcgagaa
     9301 tcgcgtccat ctaccttcgc gggagcctcg tctgcggggg gagcgcgccg tcaggcgcga
     9361 gagggcatgc tagcacgcca cgtgccgtgg agcctccgcg gccagggtcg cgagggtcgt
     9421 caccagcagc cgatgctcct cggccagcac gcgagcggcc agcgtgtcgg catcgtcgcc
     9481 cgggagcacc gggacgggag cctgggcgat gaccggcccg ccgtcgagct ccggcgtcac
     9541 gtggtggatg gtcaccccgg tctcgcggtc gccgcacgcc agtaccgacc gatgcaccgc
     9601 catccccatc atcccggctc caccgtgcgc cgggagcagg ctgggatgga tgttgatggt
     9661 cgacccgccg aaggcggcga agtacgacgg ctgcaacagc tggtcgtagc cggcgagcag
     9721 cgccaggtcg acgccggcat cggcgagggc gcggccgacc tccgcgtcgc gcgcggcggc
     9781 atcggcgaag tcgcggcgcc ggagaaccag ggccggcacc ccgtgggctg ccgccacctc
     9841 cagcgcccga acgcccggcc ggttgctgat cacgagcacc ggctccaggt cggcgatgcg
     9901 cgcggtgagc accgcctcca ggttggaccc gcgccccgag accaggacgc cgagtctcat
     9961 gcgaagcgaa cgcgcggctc gccaccgatg acgtcgacca cgcggccgat gcgccgcgcc
    10021 tccggcacgg cgtcgagcgc ggcccccgcg tggtccggcg cgaccaccac ggtcatcccg
    10081 atcccgacgt tgaacgtgcg gaccatctcg gtgtcgtcga tgtcgccgcg cgactgcagc
    10141 aggttgaaga tcggcgggac gggccacgac cccgtgtcga tctcggcccc caggccctcg
    10201 ggaagcgtgc gcgggatgtt gccctcccag ccaccgccgg tgatgtgcgc catggcccgc
    10261 acgtcgacgg cgcgccgcag cgcgcggacc tcgtccagat aggaccgatg cggctcaagc
    10321 agcgcgtcgc cgatggtgcc gccgccgtgc ggcatcgggt cccgccagac ggtctccggc
    10381 aggacgcggc gcgcgagggt gtagccgttg gtgtgcaggc ccgacgacgg gaggccgagc
    10441 acgacgtcgc cggcgcggac ccgcgagccg tcgatcagct cggagcgccc caccaccccg
    10501 acgatgaagc cggccaggtc gtagtcatcg tcccggtaca cgtcgggcat ctgggcggtc
    10561 tcgccgccga tcaacgggat gccggccgcg gcgcagccgg cggcaacgcc accgacgatc
    10621 gactccacca cgtccgcgcg caggtcgccg agggccaggt agtccaggaa gaagagtgga
    10681 tccgcgccgg tgaccaggat gtcgttgagg cagcagttca ccaggtcgac gccgatggtg
    10741 tcgtgacggc ccaggccgag cgccaccttc agcttggttc ccacgccgtc cgtgctgctg
    10801 accagcaccg gatcggggtg ggtcgacggg ttgaaacgga agagccccgc gaagccgccg
    10861 acgccggcga gcaccgcggg cgtcgcggtc gacgccgcgg cccgcgcgat gcgctccacc
    10921 gcgcgctcgg cctcggccac gtccacgccg gcctgccggt acgtgcgcgc catcggtcag
    10981 cggcgctcca tggccagctt gtcgagctcc atcggcacgg cggtcggata gcggccatcg
    11041 aagcaggcgg tgcagtgccc gccctcgccc gcgccgttcg ccccgcgcac cgccgcgagc
    11101 aggccctccc gggacagaaa cccgagcgag tcggcaccga tgacccggcg gatcgcctcc
    11161 gtcgagtgcg tcgcggcgat gagctcgccc cggcgtccgg tgtcgacgcc catgtagcac
    11221 gggtgccgca tctccggcga gtggatgcgg acgtgcacct ccgccgcgcc ggcccggcgc
    11281 agcaggttga cgatcggacc ggtggtcgtg ccacgcacga tcgagtcgtc cacgaccacc
    11341 acccgcttcc cggagagcag ctcggggagc gggttgaact tcagccgcac cgcctcctcg
    11401 cgtccgtcct gcgcgggctg gatgaaggtt cggccgacgt accgtgactt gatgaggccc
    11461 tcgcggtacg gcagcccgct ctcctcggcg tagccgatgg cggccgggat ggccgagtcc
    11521 ggcacggcga tgaccaggtc ggcgccctcc gcgggctgct cgcgggccag gacgcgaccc
    11581 atctcgagcc gcgcctcgta cagcagccgg ccgccgatca ggctgtccgg ccgggccagg
    11641 tagacgtact cgaagacgca cagccgctcg cgctgcggcg tcgccaggct ggaccgatgc
    11701 gagtgcaccc cctcggcgtc gatggtgacc acctcgccgg gctcgatgtc gcgcaccagc
    11761 tgcgcgccca tggcgtcgag cgccgtgctc tccgacgcga tggcccaccc accgttcggg
    11821 agccgcccga gggcgagcgg ccggaagccg acggggtcgc gcccggccac gagcgcatcg
    11881 gccgtgagca tggtgaaggt gaaggcgccg gtcgcgcgat ggaaggcgtg gtcgacgcgc
    11941 tgcccccacg tgcggcccgg cgcgtgggcg atgagccggg cgaggacctc cgtgtcggac
    12001 gtcgacgcga agtcgatccc ctgttccgac agctcgtcgc gcagccagcg cgcgttggtg
    12061 aggttgccgt tgtgcccgag catcagctcg ccgcggtccg actgggcgta caccggctgg
    12121 acgttcggca gggtgttcga gccggtcgtg ctgtaccggg tgtgcccgat ggccgccacc
    12181 ggcggacgcc cctggccgcc gtcctcgagg cgcagcaacg tctcggcgtc gaagacgccg
    12241 gccaccaggc ccaggcgctt gtggagtgag aggtgctccc cgtcgctcac ggcgatgccg
    12301 gcggactcct ggccgcggtg ctggagcgcg tggagtccca ggaacgcgag gcgggcgacc
    12361 ggggcgcccg gcgaccaggc gccgacgacg ccgcactcct cgcgcggacc gtggggagcg
    12421 taggtgggcg gaagctcgtc gatcgagatc aggggcggga cgatcgacgg atccacgggc
    12481 tggtcagggg acgcggtgcg gggacggcac gacggcatga tatccgaggc ggcgctgccg
    12541 gcgacggcgc gcttcggcgg cgcggagggc ttcacgccga cggtaccccg cgggtactgc
    12601 actcctccgc accaggcgcc cggtggtgct cgaccgcgcc ggtgcgagga cgcactccgg
    12661 gaccatcgac ccatggtgaa ggatgacctc ggcggcccag actcgtcgtc gtgctcacct
    12721 tcgtccgtgc cctgggcttc ctggcaccct tcgccatcgc ggccgtgctg accatcggcc
    12781 tgggcatcgc cgcccagacg gagcccgacc agcgattcaa gctcgtcgcc gtcctggtga
    12841 cggccgccgc ggccggcatg accaccatgg cgctgctggc ctggagctac gtgggctggc
    12901 gcctgaaccg catcgcccgc gccctggagg ggacgctcga gaacgacgag ccggtcgagc
    12961 tgaaggtctc cggcatcccg gccgagcggc gcctggcgcg cgccttcaac gcgaccgccg
    13021 gcgccttctt ccagaccgag gcccgcgcca cccacgaccg gctgaccggc attccgaacc
    13081 gcgagacatt gctcgcgacc ctcaacgtgg agatcgagcg ggccgcgcgc cactacaagc
    13141 cgctgagcgt cgccttcatc gacatcgacc gcttcaagcc gatcaacgac acctacggcc
    13201 acaagtcggg agacgcggtc ctgcgccagg tggcgaccat catcgccgac gccatccgca
    13261 tcagcgacac gttcggccgg tacggcggtg aggagttcat gctcatcctg cccgagacca
    13321 cgcccgacga cgcggtcggc ctggccgagg agctgcgcgc gctggtcatg caggagccgc
    13381 tgcgcgtggc cgacggccag accatccagg tcaccatcag cgccggcatc gccggcggac
    13441 gaggatcgga gctgcagctc gacatgctgg tcgaccgggc cgatgcggcc atgtacgccg
    13501 ccaagtcgct cgggcgcaac cgcacctacc agttccgcga cgtcgacgag gaggcgcccg
    13561 tccggcgcgc
//
LOCUS       DASTYP010000008         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_765729, whole genome shotgun sequence.
ACCESSION   DASTYP010000008 DASTYP010000000
VERSION     DASTYP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765729"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /gene="thrS"
                     /locus_tag="VFV59_00390"
     CDS             complement(<1..1548)
                     /gene="thrS"
                     /locus_tag="VFV59_00390"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5324323.1"
                     /translation="MAIDADEIGQDASAALASGREDHLAAMRHSAAHMMAAAVLELFP
                     DAKLGIGPAIKDGFYYDFDLPRPLTPDDLQQIEDKMRGQQAAALAFERSRDLPRDEAI
                     ALNRERNQPFKIEIIEDLPESEGPTVSFYRHGSFEDLCRGGHLDSTGDLGAFKLLHTA
                     GAYWRGDETRPMLQRIYGTAWDTQDELDRYLWRIEEARKRDHRKLGRELDLYTFHPES
                     PAAPFLHPRGMSIWRALEEWSREVRRAAGFHEVRTPSLVRKELWETSGHWANYQDNMF
                     VLDDYDHVSGLKPMNCPEEILIYRTRGRSYRDLPMRFADYSTLFRKERTGVLAGMFRV
                     RQLTQDDSHVFCRDDQVDDELRLALSLVRKQYAPFGFEPKAKLATRPEKRIGSDEFWE
                     RAEARLRDRLDEDGMVYSLDEGGGAFYGPKIDIFIEDALGREWQMATIQADYQLPQRF
                     DLEYRAADGGFERPVIVHYAIYGSFERFIGIITEEYAGAFPLWLAPVQVMIVPIADRH
                     LQYAEQVR"
     gene            complement(1597..1833)
                     /locus_tag="VFV59_00395"
     CDS             complement(1597..1833)
                     /locus_tag="VFV59_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324324.1"
                     /translation="MINRDNPQGPGQGPLDRPARDAGDPIGDDAYPGGSGMLPPTADM
                     DDPIPETGMPGEPRAEPDQLEEEEELGPEDEPLP"
     gene            complement(1899..2414)
                     /gene="paaD"
                     /locus_tag="VFV59_00400"
     CDS             complement(1899..2414)
                     /gene="paaD"
                     /locus_tag="VFV59_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEU5324325.1"
                     /translation="MTPATAARRTEEVWAALREIADPEIPAISVVDLGVIGDVDVTDE
                     RVRVELLPTFVGCPAIGVMEAQIGERLRTLGVAPEVEVRVVFDPPWTSDRISPEGRER
                     LRQSGFAPPVAIAGADVGGLDELAILPIAECPYCGSRNTTLDNAFGPTLCRAIYHCAD
                     CRQPFEQFKRV"
     gene            complement(2411..>2602)
                     /locus_tag="VFV59_00405"
     CDS             complement(2411..>2602)
                     /locus_tag="VFV59_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324326.1"
                     /translation="LPAGLAELHRTWDERMRPILEPLTGPLPNATPRTDGRTQRTDDF
                     RWLHREFTSVARSQEGATW"
BASE COUNT          367 a          906 c          918 g          411 t
ORIGIN      
        1 gcgcacctgc tcggcgtact ggaggtgacg gtcggcgatc ggcacgatca tcacctggac
       61 cggcgccagc cacagcggga aggcccccgc gtactcctcg gtgatgatgc cgatgaagcg
      121 ctcgaagctg ccgtagatcg cgtagtgcac gatgaccggg cgctcgaatc ccccatcggc
      181 cgcgcggtat tcgaggtcga accgctgcgg cagctggtag tccgcctgga tggtcgccat
      241 ctgccactcg cgaccgagcg catcctcgat gaagatgtcg atcttcgggc cgtagaaggc
      301 accgcccccc tcgtcgaggc tgtagaccat gccgtcctcg tcgagccggt ctcgcagccg
      361 ggcctcggcg cgctcccaga actcgtcgga gccgatgcgc ttctccggcc gggtcgccaa
      421 cttcgccttc ggctcgaagc cgaacggcgc gtactgcttc cggacgagcg agagggcaag
      481 gcgcagctcg tcgtcgacct ggtcgtcgcg gcagaagacg tgcgagtcgt cctgggtcag
      541 ctgtcggacc cggaacatcc cggcgaggac gcccgtgcgc tccttccgga acagggtcga
      601 gtagtccgcg aagcgcatcg gcaggtcgcg atacgagcga ccccgggtgc gatagatgag
      661 gatctcctcg gggcagttca tcggcttcag gccgctgacg tggtcgtagt cgtcgaggac
      721 gaacatgttg tcctggtagt tcgcccagtg gcccgacgtc tcccacagct ccttgcggac
      781 caggctgggc gtgcggacct cgtggaagcc ggcagcgcgt cgcacctcgc gcgaccactc
      841 ctcgagcgcc cgccagatgg acatcccacg cgggtggagg aacggggcgg ccggcgactc
      901 gggatggaag gtatacaggt ccagctcgcg gccgagcttg cggtggtccc gcttgcgcgc
      961 ttcctcgatg cgccacaggt agcggtccag ctcatcctgg gtatcccagg cggtgccgta
     1021 gatgcgctgc agcatgggtc gggtctcgtc gccgcgccag taggcgccgg cggtgtgcag
     1081 cagcttgaac gcgccgagat cgccggtgga gtcgaggtgg ccgccgcggc acaggtcctc
     1141 gaacgagccg tggcggtaga agctgacggt cggaccctcg gactcgggca ggtcctcgat
     1201 gatctcgatc ttgaacggct ggttccgctc gcggttcagc gcgatcgcct cgtcgcgggg
     1261 caggtcgcgg gatcgctcga acgccagcgc cgcggcctgc tggccgcgca tcttgtcctc
     1321 gatctgctgg aggtcgtcgg gggtcagcgg acgcggcagg tcgaagtcgt agtagaagcc
     1381 gtccttgatg gccggaccga tgccgagctt cgcgtcggga aacagctcga gcacggccgc
     1441 cgccatcatg tgggcggcgc tgtggcgcat ggccgccagg tgatcctcgc ggcccgaggc
     1501 gagcgccgcg ctcgcgtcct ggccgatctc gtcagcgtcg atagccatgc ggcgaagggt
     1561 agcgcatgga ggcggtcgcg cggctcgtcg ggctcctcag gggagcggct cgtcctccgg
     1621 ccccagctcc tcttcctcct ccagctggtc cggctccgcg cgcggctcac ccggcatccc
     1681 cgtctcgggg atgggatcgt ccatgtcggc ggtcggcggc agcatcccgg agccgccggg
     1741 gtaggcgtcg tcgccgatgg gatcgccggc gtcgcgcgcc ggccgatcca gcgggccctg
     1801 gcccggaccc tgggggttgt cgcggttgat catgccgctg accgatgcaa tcggcgggcc
     1861 atccaccgcg ctggcctgcg gctacctccg gccgaccgtc acactcgctt gaactgctcg
     1921 aacggctgcc ggcaatcggc acagtggtag atggcccggc acagggtcgg cccgaacgca
     1981 ttatcgaggg tcgtgttgcg ggaaccgcag taggggcact cggcgatcgg caggatggcc
     2041 agctcgtcca gcccgccgac gtccgccccg gcgatcgcta ccggcggtgc gaagccgctc
     2101 tggcgcaacc gttcccgacc ctcgggcgag atgcggtccg aggtccacgg cggatcgaag
     2161 acgacgcgca cctccacctc cggtgcgacg cccagcgtcc gcaggcgctc cccgatctgc
     2221 gcctccatga cgccgatggc cgggcagccg acgaacgtcg gcagcagctc gacgcggacg
     2281 cgctcatcgg tcacgtccac gtcaccgatg acgccgaggt cgaccacgct gatcgccggg
     2341 atctccggat ccgcgatctc gcgcagcgcc gcccagacct cctcggttcg tcgggcggcg
     2401 gtcgccggcg tcaccacgtc gcaccttcct gcgaccgcgc aacactggtg aactcgcggt
     2461 gcagccagcg gaagtcgtcc gtgcgctgcg tccggccgtc ggtgcggggc gtggcgttgg
     2521 gcagcggtcc ggtgagcggc tccaggatcg gccgcatccg ctcatcccac gtccgatgca
     2581 gctcggccag ccctgccggc ag
//
LOCUS       DASTYP010000009         6264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1057293, whole genome shotgun sequence.
ACCESSION   DASTYP010000009 DASTYP010000000
VERSION     DASTYP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057293"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFV59_00410"
     CDS             <1..927
                     /locus_tag="VFV59_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324327.1"
                     /translation="TYYLSALAVAIGFRMNLFNIGVDGQYRLAAFCAAVVGGWVELPK
                     PLHILVIVVTAMLVGAMWAGVAAALKVTRGVSEVISTIMLNAIATALIAFMLREVAVE
                     IAGSNNIGTEPISESGAFPGLPLVPGSSLDVFGFIIVAALAGATYWLVLGRTRFGFDL
                     RATGRSESAAVASGVNVKQMILVSMLLSGAVAGLVGMPQLLGGDTRTYSLDFPAGLGF
                     TGIAIALLGRNNAIGMAFSALLFSFLDASSIILDINDISKEIVLIMQGTIVLSVVVAY
                     ELVRRYSIVAEQRRVSRELAEEHEAGREEVSA"
     gene            924..2213
                     /locus_tag="VFV59_00415"
     CDS             924..2213
                     /locus_tag="VFV59_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324328.1"
                     /translation="MSLLGPQPTAGTEQPGGPQPVSRTRRLPRAGAPRILLALAVLIV
                     VLSVVSALAGAEDLTSSGTIGAALALAVPIGMAGLGGLWAERAGVVNIGLEGMMILGT
                     WGAGWAGYQWGPWTGVLVGVLMGAIGGLVHALATVTFGVDHIVSGVAINILGLGVTQF
                     LSVVAFEGVTGGGESQSPPIADIAEISIPGVAEPLLELQEKQIFLISDIAGIARGLFT
                     GVSLLTVVAILLVIGTYFVLWRTAFGLRLRSCGESPIAAESLGVNVYLMKYVAVIASG
                     AFAGIGGAFLAIVAGNGYREGQTAGRGFIGLAAMIFGNWRPGGLAAGAGLFGYTDALQ
                     LRRGGENVHALLLLVGVLLVGAAIWLAVRRQLLPAVILSGAGALFLVWFLFTDELPEQ
                     FTTMTPYVTTLLVLALASQRLRMPAADGQPYRRGEGG"
     gene            2206..2610
                     /locus_tag="VFV59_00420"
     CDS             2206..2610
                     /locus_tag="VFV59_00420"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5324329.1"
                     /translation="MAEPRVDWAALRAAAVDVMGRAYAPYSEFRVGAAGLVDDGRIVA
                     GCNVENAAYGVGLCAECGLVSALHASGGGRLVAAVCVDAHGEPLMPCGRCRQLLWEQG
                     GPDMLLETVRGVRPMSEVLPDAFGREDLERRS"
     gene            2607..3905
                     /locus_tag="VFV59_00425"
     CDS             2607..3905
                     /locus_tag="VFV59_00425"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEU5324330.1"
                     /translation="MSGFDAVEVIRAKRDRAPLTDAQIDWVIAAYTRGEVADEQMSAL
                     AMAILLNGMDRREISRWTDAMIASGIRMSFPAEALGGRPTTDKHSTGGVGDKITLPLA
                     PLVAACGAAVPQLSGRGLGHTGGTLDKLESIPGWRATLSREEMLAQLGEVGAVVCAAS
                     EDLAPADKKLYALRDVTGTVEAIPLIASSIMSKKIAEGTGALVLDVKVGSGAFMKDLD
                     SARELARTMVALGADHDVRTVALLTNMATPLGLTAGNALEVRESVEVLAGGGPPDVVE
                     LTLTLAREMLAAAGLDGEDPAQRLADGSAMDVWRRMIRAQGGDPDATLPTAQETQVVA
                     APADGVLVHLDAYAVGVAAWRLGAGRARKEDPVQAGAGVEMHAKPGATVRGGEPLFTL
                     HTDEPERFARAEEVLADAVTIAPDDSRPDLPALVLDRVAE"
     gene            3923..4282
                     /locus_tag="VFV59_00430"
     CDS             3923..4282
                     /locus_tag="VFV59_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEU5324331.1"
                     /translation="MPQLIQRPTRIPVPGGKVIHEYVGRVTSGTSSVSVAHMKAPAGW
                     EEPAQTPEFDEVTLVLMGTVVVEHDGGTLEVPAGQAVLTRSGERVRYRVGDEGAEYVA
                     VCLPAFDPDLAHRDPEG"
     gene            complement(4293..4697)
                     /locus_tag="VFV59_00435"
     CDS             complement(4293..4697)
                     /locus_tag="VFV59_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324332.1"
                     /translation="MGAREDVRIIRPAAILDERAAAQVLQQLRRLDVSAGGVWNATSS
                     LWQRYDAPWDGVGGTRGEAQLLGSIAVMYDSPHRHEITIYKVTVTENGLDQGWTVESL
                     CDDALIWAGLTIATCPRAELGSPPEADPFRRR"
     gene            4767..5870
                     /locus_tag="VFV59_00440"
     CDS             4767..5870
                     /locus_tag="VFV59_00440"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU5324333.1"
                     /translation="MTNSLTDTLLQQAPKVLLHDHLDGGLRPATVVDLARETGYGALP
                     TTDVDKLGRWFVAAASSGSLEDYLETFAHTVGVMQTGDALARVAAECAEDLAADGVVY
                     AEVRYAPELHVTAGLSLEEVVSAVNEGFREGERRAAAAGNRIRVGALLTAMRHAARSM
                     EIAELVVRYRDEGVVGFDIAGAEAGYPPTRHLDAFEYLQRENAHFTIHAGEGFGLPSI
                     WQAIQWCGADRLGHGVRIIDDIEGDLTGPASGVRLGRLAAYVRDKRIPLEMCPTSNVM
                     TGAAKSIEEHPIGLLRRLNFRVTINTDNRLMSGTSMTREFRQLAQAFDYHLDDFRWFT
                     INAMKSAFIPFDERLAFIDDVIKPRYAALARSY"
     gene            complement(6066..>6264)
                     /locus_tag="VFV59_00445"
     CDS             complement(6066..>6264)
                     /locus_tag="VFV59_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324334.1"
                     /translation="ADHGDMFLTDCRACSRRELRGTRSIELLVNTDHGIGLVYRCTGC
                     GASNVLRSSSTSAAPSPLVAA"
BASE COUNT          836 a         2214 c         2275 g          939 t
ORIGIN      
        1 acctactacc tgtccgcgct ggccgtcgcc atcggcttcc ggatgaacct gttcaacatc
       61 ggcgtggacg gccagtaccg cctcgcggcc ttctgcgccg cggtcgtggg cggctgggtc
      121 gagctgccca agccgctgca catcctcgtc atcgtcgtca ccgcgatgct ggtcggcgcg
      181 atgtgggcag gggtcgccgc cgccctgaag gtgacccgcg gggtcagcga ggtcatctcg
      241 accatcatgc tgaacgccat cgccaccgcg ctcatcgcgt tcatgctgcg cgaggtcgca
      301 gtcgagatcg ccggcagcaa caacatcggc accgagccga tctccgagtc cggcgccttc
      361 cccgggctgc cgttggttcc aggcagctcg ctggacgtct tcgggttcat catcgtcgcg
      421 gcactcgccg gggcgaccta ctggctggtc ctcggccgta cgcggttcgg cttcgacctg
      481 cgcgccaccg gacggtccga gagcgcagcc gtggccagcg gcgtcaacgt caagcagatg
      541 atcctggtct cgatgctgct ctccggcgcg gtggccggcc tggtcggcat gccccagctg
      601 ctcggcggtg acacccgcac ctacagcctc gacttccccg caggactggg cttcaccggc
      661 atcgcgatcg ccctgctcgg ccgcaacaac gcgatcggca tggccttcag cgcactgctg
      721 ttctcgttcc tcgacgcgtc atcgatcatc ctcgacatca acgacatctc caaggagatc
      781 gtcctgatca tgcagggcac catcgtgctg tccgtcgtcg tcgcgtacga gctggtgcgc
      841 cgctactcga tcgtcgccga gcagcgtcgc gtcagccgtg agctcgcgga ggaacacgag
      901 gccgggcgag aggaggtcag cgcatgagtc tcctcggacc ccagccgacc gccggcactg
      961 agcaaccagg cggcccgcag ccggtctcgc gcactcgtcg cctgccgcgc gccggagcgc
     1021 cgaggatcct cctcgcgctc gccgttctca tcgtcgtgct cagcgtggtg agcgccctcg
     1081 ccggtgccga ggacctgacg tccagcggca ccatcggcgc ggcgctggcg ctggccgtgc
     1141 ccatcggcat ggccggcctg ggcggactct gggccgagcg tgccggcgtg gtcaacatcg
     1201 gcctcgaggg catgatgatc ctcgggacgt ggggtgccgg ctgggccggt taccagtggg
     1261 ggccgtggac cggagtcctg gtcggcgtcc tgatgggcgc catcggcggg ctggtccacg
     1321 cgctcgcgac cgtgaccttc ggcgtcgatc acatcgtctc cggcgttgcg atcaacatcc
     1381 tcggcctggg cgtgacccag ttcctctcgg tcgtcgcctt cgaaggcgtg acgggcggtg
     1441 gcgagtcgca gtcgccgccg atcgccgaca tcgccgagat ctccatcccc ggcgtcgccg
     1501 aaccgctcct ggagctgcag gagaagcaga tcttcctgat ctccgacatc gccgggatcg
     1561 cgcgcggtct cttcaccggg gtgtcgttgc tcaccgtcgt cgcgatcctg ctcgtgatcg
     1621 ggacctactt cgtcctgtgg cgcacggcct tcggcctgcg gctgcggtcc tgcggtgaga
     1681 gcccgatcgc ggccgagtcg ctcggcgtga acgtctacct gatgaagtac gtcgcggtga
     1741 tcgcatccgg ggcgttcgcc gggatcggcg gtgccttcct cgccattgtg gccggcaacg
     1801 gctaccgcga gggacagacc gcgggtcgcg gcttcatcgg cctcgcggcg atgatcttcg
     1861 gcaactggcg accgggcggg ctcgcggccg gcgcgggtct gttcggctac accgacgccc
     1921 tccagctgcg ccggggcggc gagaacgtgc atgccctgct gctgctcgtc ggcgtcctgc
     1981 tcgtcggcgc cgcgatctgg ctggccgtgc gccgccagct gttgcctgca gtgatcctgt
     2041 ccggcgccgg cgcgctgttc ctggtgtggt tcctcttcac cgacgagctg ccggagcagt
     2101 tcaccacgat gacgccgtac gtcaccacgc tgctcgtcct cgccctcgcc tcccaacgac
     2161 tacgcatgcc ggcggccgac ggacagccct accgccgggg cgagggtggc tgagccccgg
     2221 gtcgactggg ctgcgctgcg cgccgctgcc gtcgacgtga tgggcagggc gtacgcgccg
     2281 tactccgagt tccgcgtcgg cgcggcgggc ctggtcgacg acggccgcat cgtcgcgggg
     2341 tgcaacgtgg agaacgcggc gtacggcgtg ggcctctgcg ccgagtgcgg tctggtctcc
     2401 gcgctgcacg cgagcggggg cgggcgtctg gtggccgccg tgtgcgtcga cgctcacggc
     2461 gagccgctga tgccctgcgg ccgctgccgg cagctgctct gggagcaggg cggcccggac
     2521 atgctgctcg agaccgtgcg cggcgtacgg ccgatgagcg aggtgctgcc cgacgcgttc
     2581 ggccgcgagg acctggagag acgttcgtga gcggtttcga cgcggtcgag gtcatccgcg
     2641 ccaagcgtga ccgggcgccg ctcaccgacg cgcagatcga ctgggtcatc gcggcctaca
     2701 cccggggcga ggtggccgac gagcagatgt cggcactcgc gatggcgatc ctgctcaacg
     2761 gcatggaccg ccgcgagatc tcccgctgga ccgacgcgat gatcgcctcg ggcatccgga
     2821 tgtccttccc cgcggaggcc ctgggcggga ggccgacgac ggacaagcac tcgaccggcg
     2881 gcgtgggcga caagatcacg ctcccgctgg ctccgctggt cgccgcctgc ggtgctgccg
     2941 tgccgcagct gtccggccgc ggcctcggcc acaccggcgg caccctggac aagctcgagt
     3001 cgatccccgg ctggcgggcc acgctgtccc gcgaggagat gctcgcccag ctcggcgagg
     3061 tcggtgcggt ggtctgcgcc gccagcgagg acctcgcccc ggcggacaag aagctctacg
     3121 cgctgcgcga cgtcaccggc acggtcgagg cgatcccgct catcgccagc tcgatcatga
     3181 gcaagaagat cgccgagggg acgggcgcgc tcgtcctcga cgtcaaggtc gggtccggcg
     3241 ccttcatgaa ggacctcgac tcggcccgtg agctcgcccg cacgatggtc gcgctcggcg
     3301 ccgaccacga cgtacgcacg gtggcgctgc tcaccaacat ggcgacgccg ctcgggctga
     3361 ccgccggcaa cgcgctcgag gtgcgcgagt cggtggaggt gctcgccggc ggcggtccgc
     3421 cggacgtcgt ggagctcacc ctgaccctgg cgcgcgagat gctcgccgcc gcgggcctcg
     3481 acggcgagga ccccgcacag cggctggccg acggatcggc gatggacgtc tggcggcgga
     3541 tgatccgtgc acaggggggc gacccggacg cgacgctgcc gacggcccag gagacccagg
     3601 tggtcgccgc gccggccgac ggcgtgctgg tccacctcga cgcgtacgcc gtgggcgtcg
     3661 ccgcctggcg gctcggggcg ggccgggccc gcaaggagga cccggtgcag gccggcgccg
     3721 gcgtcgagat gcacgcgaag ccgggagcga ccgtccgcgg cggcgagccg ctgttcaccc
     3781 tgcacaccga cgagcccgag cggttcgccc gggccgagga ggtgctggcc gacgcggtga
     3841 ccatcgcgcc ggacgactcg cgacccgacc tgcccgccct ggtcctcgac cgcgtcgccg
     3901 agtagccgga ggaggcggcc ccgtgcccca gctgatccag cgccccaccc gcatcccggt
     3961 cccgggcggc aaggtcatcc acgagtacgt cgggcgcgtc acgtccggca cgtcgtcggt
     4021 gtcggtggcg cacatgaagg cgccggccgg ctgggaggag ccggcgcaga ccccggagtt
     4081 cgacgaggtc acgctcgtgc tcatgggcac cgtggtggtc gagcacgacg gcggaacgct
     4141 ggaggtcccg gcgggccagg cggtcctcac ccgatcgggt gagcgggtgc gctacagggt
     4201 gggggacgag ggcgcggagt acgtcgcggt gtgcctgccc gccttcgacc cggacctcgc
     4261 ccaccgcgat ccggagggct gaccggagcg gctcagcggc ggcggaacgg gtcggcctcg
     4321 ggcggagagc ccagctcggc ccgggggcag gtggcgatcg tgaggccggc ccagatcagc
     4381 gcgtcgtcgc acaggctctc gacggtccag ccctggtcca ggccgttctc ggtgaccgtg
     4441 accttgtaga tcgtgatctc gtggcggtgc gggctgtcgt acatcacggc gatggagccg
     4501 agcagctggg cctcgccgcg cgtgcccccc accccgtccc acggcgcgtc gtagcgctgc
     4561 cagagcgacg acgtcgcgtt ccagaccccg ccggcggaca cgtcgaggcg ccgcagctgc
     4621 tggagcacct gcgcggcggc ccgctcgtcc aggatcgccg cagggcggat gatccggacg
     4681 tcctcgcgtg cacccaccct catagcctct ttcttcgtgt tcagggaccc cgaagttgag
     4741 agtctgtcag acggtaacgt gcgctcgtga ccaactcttt gactgacacc ctgttgcagc
     4801 aggctcccaa ggtgctcctg cacgaccacc tggatggcgg cttgcgcccg gccacggtgg
     4861 tggacctggc ccgggagacg gggtacggcg ccctgccgac gaccgatgtc gacaagctgg
     4921 gtcgatggtt cgtcgcggcc gcgagctcgg ggtccctcga ggactacctg gagacgttcg
     4981 cccacaccgt cggggtcatg cagaccgggg acgccctggc ccgggtggcc gccgagtgcg
     5041 cggaggacct cgccgccgac ggggtggtgt acgccgaggt gcgctacgca ccggagctgc
     5101 acgtgacggc cggactgtcc ctggaggagg tcgtctccgc cgtcaacgag ggcttccgtg
     5161 agggcgagcg gcgggctgcc gctgcgggca accggatccg ggtcggcgcg ctgctcaccg
     5221 cgatgcggca cgcggcgcgg tccatggaga tcgccgagct cgtcgtgcgc taccgcgacg
     5281 agggtgtcgt gggcttcgac atcgccgggg cagaggccgg ctacccgccg acccgtcacc
     5341 tggacgcgtt cgagtacctc cagcgggaga acgcccactt caccatccac gcgggggagg
     5401 gcttcgggct gccgtcgatc tggcaggcga tccagtggtg cggcgccgac cggctcggtc
     5461 acggcgtacg catcatcgac gacatcgagg gggacctgac cggtccggcc tcaggcgtgc
     5521 ggctcgggcg cctcgcggcg tacgtccggg acaagcggat cccgctggag atgtgcccga
     5581 cctccaacgt gatgaccggg gcggcgaagt cgatcgagga gcacccgatc gggctgctgc
     5641 gccggctgaa cttccgcgtg acgatcaaca ccgacaaccg cttgatgagc gggacctcga
     5701 tgaccaggga gttcaggcag ctggcgcagg cgttcgacta ccacctcgac gacttccggt
     5761 ggttcaccat caacgcgatg aagtcggcgt tcatcccgtt cgacgagcgt ctcgcgttca
     5821 tcgacgacgt gatcaagccc cggtacgccg cgctggccag gagctactga ccgggcgccg
     5881 cgcgcgccgg gcaccctccg aagcggaccg gctgcacgtc gcggcacgcc gcgccgggcc
     5941 ggtccacttc gaagcggacc ggctggacgt cgcggcacgc cgcgcgccgc gcaccctccg
     6001 aagcggaccg gctggacgtc gcggcacgcc gcgccgggcc ggtccgcttc gctgggtgac
     6061 cggggtcagg ccgcgacgag tggggacggc gcagccgagg tgctggagga gcggagcacg
     6121 ttgctggccc cgcagccggt gcagcggtag accaggccga tgccgtggtc ggtgttgacg
     6181 aggagctcga tggagcgggt accccgcagc tcgcggcggc tgcaggcccg gcagtcggtg
     6241 aggaacatgt ctccatggtc ggca
//
LOCUS       DASTYP010000010        23566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1494334, whole genome shotgun sequence.
ACCESSION   DASTYP010000010 DASTYP010000000
VERSION     DASTYP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 23566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23566
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494334"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(495..1184)
                     /locus_tag="VFV59_00450"
     CDS             complement(495..1184)
                     /locus_tag="VFV59_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324335.1"
                     /translation="MINRLKGLGIVLALIGIAFIGAGAFAYLKVQEGTASLQKFSAAQ
                     NVTLSYNEDGQLVDRGETAGAEAIMGLLKDDWGYPVVASELDPNDPVVNTASEYMYQM
                     ATVAFHTLHGTQTVVLEEDFTAPDGTVYTAGTPYEIPVDGRYWAAFDRSNPIDAAVRA
                     QAWTGVAHALIGELGVGTVTAQALQLGLALAGLFAGVGATFILAGGGLVWASRGAEVP
                     AFETKRASVIA"
     gene            complement(1442..1993)
                     /locus_tag="VFV59_00455"
     CDS             complement(1442..1993)
                     /locus_tag="VFV59_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324336.1"
                     /translation="MNDHRDESLIDKIKNAFGMGDDGTDSPADDFRDDVPTTAATGTA
                     TVSPEASAPQAGFSEPFEEDLGDPANRPTGPDYGATDGPVETGFGTAATRVDDFGAGL
                     DEETFEGTAATDTGPRVTPAGYDDPYETVAGPGPMGAGSTADADAGDAWTRGEAASGT
                     SPFEGDQRREGEVIEQERRETGI"
     gene            2209..3237
                     /locus_tag="VFV59_00460"
     CDS             2209..3237
                     /locus_tag="VFV59_00460"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5324337.1"
                     /translation="MLILAACSQNAGSSASEEPSQAPASEAPASEGAESEAPAASEEP
                     ERSGSARIGIGGWSIAYVPTAIVIDRMNELGYDIEAVETSGTSAQMQAATQGELDITS
                     VSAAATMTAIDAGLDSKFFLTRNLNEFVFVAKSEFDSCESLDGQRVAIHAQTDITGLL
                     TINWFEKNCPDANPQIQIVEGSENRLAGLLQDQLDASPVDIGDAIQLERERPGEFTVL
                     ASFVDEFPVLQGVYAASPSWLAENEQLVKDFIQLHLEVWDEFYADPTSILPKAEELLP
                     EIDPEVLAQLAEEYADKEIFPPDGDLAEEETQYTLDFFSEAAGFENVGSYEDIADRQY
                     LDAVLEAQ"
     gene            3340..4143
                     /locus_tag="VFV59_00465"
     CDS             3340..4143
                     /locus_tag="VFV59_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324338.1"
                     /translation="MTTLVARPRTFARVQGGLKKVLLRSAILVVLIVLWQMGGNDEVR
                     LGLPTFVGTMRALGEMLADGSLLEALWITNQALVVGYLLAIVVSLPLGVAMGSSPAFE
                     RVAGPYLIILQAAPMIALVPIVQAGLGLTFAARVAIVFLFSFIYMTINTMVGVRSVDP
                     ALKEMSRSFLASASQRMRFVILPAAVPGIMAGVRLGLGRAMIGMVVAELALIGAGVGS
                     LILDYQVRFQPAYVFAIILVVVLEGVLLMEIARRLELRLSQWKGSTVLE"
     gene            4136..4939
                     /locus_tag="VFV59_00470"
     CDS             4136..4939
                     /locus_tag="VFV59_00470"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324339.1"
                     /translation="MSKPAIEIRGLGKMYRESDGDAAFRALGDVNLTIGDGEFVSLIG
                     PSGCGKTTLLKIIDGLIPFDEGELLVKGQPIRGPGRERAVVFQNFALLPWLNVRDNIA
                     FGLKLQGTDAAAMRQTADELISMVGLDGFADRYPRQLSGGMQQRVGLARALAVDPAIL
                     LMDEPFGALDAQTRNILQGDLLDIWDRSRKTVVFVTHAMDEAVFLSDRVVLMGTHPGR
                     VHEVIDIDLPRPRTDETRSDPRFVELSSYVWEVLRTMIGHPRRQVAAAS"
     gene            4936..5736
                     /locus_tag="VFV59_00475"
     CDS             4936..5736
                     /locus_tag="VFV59_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324340.1"
                     /translation="MTQGAAVIGQPAPRRALVPRRWLAHNGPFLLSFVLVMATWELLG
                     RTLDLLMLPPISAIFAALWQFTIDGTLADQLFVSLLTLFIGMVISILLGIAIGAAMGL
                     SRTVESALDVYVNAMMAAPMVAFVPLFILLFGLGYPTRVLTVIIFAIFPIIVNAFAGL
                     RNIDRSTVEMARSFGATKRQLLWQVRIPGAFPYLLAGIRLGTARGVKGLINGEVLVTV
                     VGLGGLVNQYGTAFTMDRLYAIIFFIIALALVLVRGVEWISRRLVPQA"
     gene            5755..7452
                     /locus_tag="VFV59_00480"
     CDS             5755..7452
                     /locus_tag="VFV59_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5324341.1"
                     /translation="MTRYGSDIVVDVLVDLGIGHVAFNPGASFRGLHDSLVDRLDDGG
                     PRLTMTLHEEIAVAAAHGYAKATGRPMAVALHDVVGLQHACMALFNAWCDRVPMLVLG
                     ATGPVDAARRRPWIDWIHTANVQGSHVRDFVKWDDQPMSVPAAVESVQRAYLLATTPP
                     QAPTYVCLPVEVQEDAVEAHAELPPVRPVGRAAPDPSAIDDVLGLIADSRRPIIVADR
                     AADRPGAFEALGELALAIGAPLLDGGARFNVATTHPNDLTGDERQVLASADLLLAFEV
                     ADLAAVLAASPEPVTAQIVDVGLSHNLVGSWAADYQRLVPVRVVLPADAGLTARALID
                     GWGRRGRARDAAEGRNREIVARHHALREEWSAVAAGSSARTPISTAWLAHEIGRAIGG
                     RDAVLSNGTLNGWARRLWDLDRPRAMLGGNGGGGIGYGIGASVGAGLAARDEGRMVVN
                     LQPDGDLLYAPSALWTMAHEALPVLTVVWNNGGYRNSEEHAERVALARSRSVDRAGIG
                     NRIEAPAVDIAVLARAYGIPAEGPVESPGDLRAALGRAVSAVADGGPALVEVRAAAR"
     gene            complement(7457..8194)
                     /locus_tag="VFV59_00485"
     CDS             complement(7457..8194)
                     /locus_tag="VFV59_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5324342.1"
                     /translation="MLNPALIVLMFAAGLVNGWTALGFAMIAAAGFALAVDPKTAVLL
                     IAVTTPFISSMQLMNHKTSYPGWRRLAPIALGGFIGVPLGAVLLGVLHADAIALLLGA
                     MAVLFVVSTFANRGPRIPAAAEPIASPVAGVAAGVANGTVGVSGPVLGIYLIALGLSP
                     AAFGFTISLLFFAMGLVRLVSLAALGQLTWAIVGTGVGLVIPALIGQQIGFRLHHWVK
                     PTHARQGALVLIGIAGITLILRGLNLS"
     gene            8260..9123
                     /locus_tag="VFV59_00490"
     CDS             8260..9123
                     /locus_tag="VFV59_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU5324343.1"
                     /translation="MTDGAAPHPAPSVSDDSGGHRDAGRTPFRGEHLVSQSAAEAIAG
                     QVRGALIEGRLQPGDRLATEPEMAEDFGVSRSTVREAIRILRGQGFVQTVRGAKGGHF
                     VVTPQTDVVAESVGETLGLWFEAGTVSVAEVDEARNLVEAACVRLAAERRTEEDLRAM
                     EDVLADAAVDDQPLRAFLDHDVRFHRCIARAARNRLLELPMTAIHLIRPRTNVLLRRH
                     DRETVRAQHRQLYEAIRDGDADAAEAAFRDHASYIAEERAAAAEERQRRPTEIAVREL
                     PELSDGEHSAT"
     gene            complement(9120..9881)
                     /locus_tag="VFV59_00495"
     CDS             complement(9120..9881)
                     /locus_tag="VFV59_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5324344.1"
                     /translation="MRLDGRCIVITGGAHGIGRAYARRCAAEGASVVIADIDGDAARQ
                     VADGIVADGGAAVGMAADVRDLESMERVVAHAEQAFEGVDGLINNAGMLTIIPISRVG
                     FESIDEREWDAVLDTNLKGTWLATRAAVPAMRRRGGGSIVNIGSGTFFLGSPTRAHYV
                     ASKGGVIGLSRVLSRELAPDNIRVNVIVPGSTLSEADPSDDVITMRSAPIANRSIKRI
                     EMPDDLTGAAVFFLSEDSAYVTGQTLVVEGGGILH"
     gene            complement(9896..10852)
                     /locus_tag="VFV59_00500"
     CDS             complement(9896..10852)
                     /locus_tag="VFV59_00500"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEU5324345.1"
                     /translation="MSPRIAVVATGGTIQNRISERVPVEAVWERIATYDGEPLPDVDL
                     EVHDILRAGSETFGPAEWQTIAETVQAACDDASVDAVLVTHGTFTAEETAYLLNLVVD
                     SSKPVVVVVSQRKHRMAGNDGDRNLLDGIRLCLTPDARGLGVLLVLNEEVHAAREVTK
                     ENQRPGGFTSAPFGPLGTIELDQPTLYRASLRRHTGRSELRRTPLASLPRVDIVSAYA
                     GADAVGVKAVVAAGAEAIVLAGFAYSGRGSDPQMETLEHVARSGVPVIFASRGRGGRI
                     PRVDGSWWVRADNLTPQKARVLTAVALAAGRGRDLQTLFDTH"
     gene            complement(10849..11718)
                     /locus_tag="VFV59_00505"
     CDS             complement(10849..11718)
                     /locus_tag="VFV59_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5324346.1"
                     /translation="MSFFSDGYRLDAELHLAVDAAHAPTVVFCPGSRVSKRTPYYHEY
                     IPRLVGAGTSVLLMDYRGWGESEGPRGQLYPWQQVADIRNAVTYLETDQRIDAGRIGV
                     FGVSFGGALGVAAAAHDARIRAVVTVLSPMDGAMMLRGSRREYEWVELLAQLAEDRRR
                     RVTTGSGGVTEHFSPPTPERSKTSALSQEAIPPIPLACLEAIAEFRPLDLVARIAPRA
                     VLLIAATLDPTCPVEHSRLAYEAAGSPKRLVEIDSTEHYGTYVKHMDLILDEATAWFD
                     RHLGPEAPRVLEA"
     gene            11822..12784
                     /locus_tag="VFV59_00510"
     CDS             11822..12784
                     /locus_tag="VFV59_00510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5324347.1"
                     /translation="MTIEFGLLLPHFSDSTTWDRLFGFAPRAEELGYESLWARDNLSF
                     HGHGFELPGDHFVDPFTTLAGVAARTERAKIGTAVLTPFRHPLITAQLVGGLDYLSKG
                     RFVLGIGPGTPRKPWEALGRQHEERVVATKETVEVLRLVSSGEDVSYEGEQFSFTDVK
                     IDPPPSPDMMVWYGGASNASIRAILDYCDGMLPGRCPFTRYDVAAERLKAGAAEIGKQ
                     VHLGSIPTVSIAPTRQEAIDKIPMQALLDTASERWKRPFEKPEDLAGAVVVGSPSDCI
                     EQVQAFVDRGIELFVVDLRLLMNEFEEAAELFAAEVIPAFRSAS"
     gene            12791..14188
                     /locus_tag="VFV59_00515"
     CDS             12791..14188
                     /locus_tag="VFV59_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5324348.1"
                     /translation="MVAEIPVYNASTGEVLQHVPEAGGEGVAAAVERARSASREWRSI
                     GAVARGQAVADFGRAVADHAEELAGIDARNAGNPITGMRRGAQQGARTLEYFAGLALE
                     LTGETIPATPDHLHYTLREPFGVVGVITPFNHPTLFATARTAAPLVAGNAVVLKPAHQ
                     TPLSALRLQELADEHLPPGVFTVVTGGGATGSALVRHPEVRRIGFTGGVETARRIASD
                     AADSGTIKRISYELGGKNAMVILPDVDIDVAAAAAVEGMNLTRVAGQSCGSTSRLFVH
                     SSICDDLRDRVVKRLEQLRFGPPEEESTQIGSLVSEQQRERVERYVRAGREEGAQLVL
                     GGERPTEPPFDRGFYFPPTLFERVRSSMTIAREEIFGPVLCMISWTDEAEMLAAVNDS
                     RYGLTASVYTNDLRAAHRLAGAIEAGYIWINDVEKRWIGVPFGGQKDSGTGTEYSIEE
                     LYSFTQNKSISVSLR"
     gene            14185..14607
                     /locus_tag="VFV59_00520"
     CDS             14185..14607
                     /locus_tag="VFV59_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5324349.1"
                     /translation="MSERVARVIRADERRPSELRYGRGSVEKIVDGAIGADRVDLHLN
                     RIRAGSAPGPYHLHTEAENVYLVLAGDVVVRIDGVDHRLGAGDAAFIPPGVPHSASNV
                     GTTDAELIEIYAPAAVDFVEVADPGAVTPTEERRPTNG"
     gene            14600..15976
                     /locus_tag="VFV59_00525"
     CDS             14600..15976
                     /locus_tag="VFV59_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5324350.1"
                     /translation="MADHATRIAGGDVLVRGQLVRADVLIDDGKIVAVVAPDVSAEAA
                     ETIDATGRVVLPGIIDTHAHTREPGYTHKEDFLTASQAAAAGGVTTMVDMPNVEPPTD
                     TVERLLEKRELADRKSIIDWGHFAAGTKLEEIPRLAEAGATGFKVFQVSGEYPHDPRL
                     AMNDEGQLLSAMRAVAETGLPLVIHPFNQSLFEKLTEEAFAAGKPNNWYTFGEVYTTE
                     AIWHTAVNTLMNLQALTGARVHLVHTHSGGSLRLIREAKERGQRVSCSVDPKYYHLTR
                     DDLMRLKGRACPGGFITEDADRMAEIWRSLRDGTIDSIDADHAPHTLEELDVMEEDAW
                     HAAMGSPQYDWMYSITLTDVHAGHFSLARAVEMLAEAPARMLGLFPKKGAILPGSDAD
                     LVLVDLDREVTLSDEGLYTKVGWTPYLGRTIKGVVTMTMLRGCVIARDRQVIGTPGFG
                     QYIAGVAQ"
     gene            15989..17017
                     /locus_tag="VFV59_00530"
     CDS             15989..17017
                     /locus_tag="VFV59_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEU5324351.1"
                     /translation="MTERPTVVVTGPWSDPGWDAALRAAGVGVIMGPSIDERPATPVD
                     EATLCEMVARADAVLVSSRERITARVLDAAPRLRLVAKATIGVERIDVEAATERGILV
                     VNSPAPENVVGLAEATIGLIVALAKQLLVKEQRIRDGGWRDATTDGLVLAGRTVGIIG
                     LGRVGSGVARRLVGWDVDVLACDPYIPVERFAAAGAGPVDLPTLLSQSDIVTLHVPLT
                     AETRGMIDAEALAAMPVGALLVNTSRGAVVDQAAIAQALQDGHLGAAALDVFETEPLP
                     PDDPIRAVPRDRVLLTPHSIGSSHASRATGTRMAIAAVLDALGGRVPPNVVNAAALHR
                     WRRRFGAE"
     gene            17140..17562
                     /locus_tag="VFV59_00535"
     CDS             17140..17562
                     /locus_tag="VFV59_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5324352.1"
                     /translation="MSLQRIKNLCVADLMTIDPIVIGPKASVSEAEELMAAKGVSGLP
                     VVDDHGRLIGVISQTDLLYLTRPAVRALMTRWETPLTVAEVMTSPPVTVRTTISLENA
                     ARVMSEHDVHRVVAVDRKGRPVGVLSASDFVQLAAIGC"
     gene            17654..18268
                     /locus_tag="VFV59_00540"
     CDS             17654..18268
                     /locus_tag="VFV59_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324353.1"
                     /translation="MSAERWICQVCWTSNRPRDEVCWRCKAPSGLAREEADAQRAARS
                     ARGEAPEPVPDLLVALPVVVFRTYARVWLRGGIGMLAVPLLMGLAGVVDVGWLVLSIG
                     LAVGLVVFGIVAGEVSDGMREREVWAFLTGIVLAVVGVIGSILAFATFAPELISPTAV
                     RWGSLLVFGGAGVAAVAGLVLLVRADRRPTDVGPGQPGPGASGA"
     gene            complement(18201..18881)
                     /locus_tag="VFV59_00545"
     CDS             complement(18201..18881)
                     /locus_tag="VFV59_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5324354.1"
                     /translation="MATTQPAPAAEPQVRRATPADISALASVLSRAFDGDPYYRFLAG
                     DAPERHARMYEGWLGILRFASAGLRETWTADDRAGVAVWLPPGDRGPSVLQGFRLLPS
                     MARLAGWRRLRAVSSATDALERRRRHHAPGPHFYLSVLGVDPDRQGEGLGTALLAPVL
                     ERCDAERIPAYLETAVARNVLLYERLGFDVVEEMVLPRTDIHGWLMRRTPRDPAGRDR
                     RRSDGGRP"
     gene            18933..20171
                     /locus_tag="VFV59_00550"
     CDS             18933..20171
                     /locus_tag="VFV59_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324355.1"
                     /translation="MTLPPTGRMLPVHVDPMREANAQRDRLVTPAFVVLAVAELAYFL
                     ASGMAIPLTPRFATGPLGADALGSGIAYGAFGVTALLLRPFAGRLSDRYGRRPFLIGG
                     AVLCSVAFAAHAVAPTLGVLVVLRLVLGVAEAFFFVAGFAMLADLAPPRRIGEALSYN
                     SLALYLGVAFGPAAGEALLDIGGFRLAWLGAAALAAVATILALVLPERHDAPRAGQPL
                     PPLLHRGAIGPSMALFVGVAGMAAFFASLSLYVPRVGLGGAGPVLLVFGLVVVGCRIA
                     FARLPDRLPPYVLGTAALVGIGAGLLVMSVVATASGLFAGAALAAAGVAFITPAMFRA
                     VVERASLSERGAALGTTSAFIDLGFASPMLFGLVAAAGGPAAAFAAAAGLALAGAAGT
                     ARAAFADRRPMQPAVADPDQ"
     gene            complement(20165..20458)
                     /locus_tag="VFV59_00555"
     CDS             complement(20165..20458)
                     /locus_tag="VFV59_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324356.1"
                     /translation="MDRERHEGAEDAEDTPLADGTGMERPNDLPAMESGDEESREPEA
                     GGDEKTAVEGASGIAATHRSFGAGSITATGGIGTDVPTDEDEEMTGSTDPRGY"
     gene            complement(20528..21190)
                     /locus_tag="VFV59_00560"
     CDS             complement(20528..21190)
                     /locus_tag="VFV59_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324357.1"
                     /translation="MPKRTAEPPPDPDRLVRQSAGTYRTEDDRFEVRSGDTGWFVVDS
                     EQTNEFGQELMHGPFATLKAAREAVPGARSPKVTALRRPRPRPRQRKGDEADAPPARR
                     KPRTWLDDLATADARRARALIRALDRRGFTDADGLVRQDREGFFPVVAARLVEDEVAR
                     LVDDWPEATRPEARALVARVLAIVTQRGGEVDAPLPGWRLVEVPHDGAGEREERPIEL
                     GE"
     gene            complement(21308..22216)
                     /locus_tag="VFV59_00565"
     CDS             complement(21308..22216)
                     /locus_tag="VFV59_00565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5324358.1"
                     /translation="MLPRLRTYAGDDDFWSVRRLFRRLLAEADFHPATWGVPRWDYWR
                     WHGVLNCGSPPPEELVTLVEDEADDLIGVVNAEDRGEAFLQLDPRCRSAWLEAAMLEA
                     AEERLANGDGLLVWTPASDERWAALLCRRGYARTSEDEHVRMRSLRDPLPAPELAPGY
                     RIRALAEGVEDFPARGDISLRVFHPVPDGNTAMTGEDYHNIQRCPLYRRDLDLVAEAD
                     DGSLAAFVTVWFDDVLRTATIEPAGTDAPHRRRGLGRALLLEGMARARWMGATRAYVG
                     SYGPGAHALYESVGLRTVEHLVGWRR"
     gene            22361..23239
                     /locus_tag="VFV59_00570"
     CDS             22361..23239
                     /locus_tag="VFV59_00570"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEU5324359.1"
                     /translation="MALYRLDEDPQLDAPVLVAAFEGWVDAGTAGTTAAAQLANDGRQ
                     VASFDSDAIYDYRARRPTLDIVDGRPVRLEWPELRLTATRIGERDLLVLAGPEPDYRW
                     RELGDEVVRLAKRLGVATWVSLGAIPAAVPHTRPVPILATASAPGLLPEGIRQGPDGL
                     LRVPSAALSMLEMSAASNGIPAIGFFAQVPHYVNATYPAASIALLNALGRYLGADLPV
                     GALASRALERRNLLDAAAATDDDTKAYVTRLEALADESRLPEGDELIADIERFLREGG
                     EEGGDGGDSGGGSRLH"
     gene            complement(23240..>23566)
                     /locus_tag="VFV59_00575"
     CDS             complement(23240..>23566)
                     /locus_tag="VFV59_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5324360.1"
                     /translation="ALSMRECVGRFPRADDGRPLRIRIGLNAGPVVAGVIGRRRFLYD
                     LWGDAVNTASRMESHGIPNEIMLTRAMYELLANRFVCEPHGPIEVKGKGVVETWLLRA
                     ERPSGA"
BASE COUNT         3334 a         8273 c         8424 g         3535 t
ORIGIN      
        1 cggtcgatgg ggtgacggtg ccggcctgga ggctcgactc atgcgaggcg ccattgcagc
       61 tggcagccgc caccggcgtc gctgcgggca gcagcgcgac gaggaacgcg acgagcgacg
      121 cccccagtct cagcttcttc atctgaccga tcgtgtggag tggagccgtg ccgcagtggc
      181 cgtgctgtcc tgggcgaacg tagcaccggc ttgctcgcct tcccgatggc ccgaaggtcc
      241 cggtcgcgcc cggacccccc ccgcctgttc caccccggtg atgcataggc ctgatcatgc
      301 ggcgcgggag cggacgctca cgtgccgccc gatcaacggc atgaatcggg cgctgcgcca
      361 acgccccttg gcgcgcactg tgatcggtgg gagctatcgc ccgccgagtt cctgcacatg
      421 acgaaacagg gagcccgggt gggctccctg ttcgggtccg tggtggtgga gggtgacccc
      481 tccgggatcg gtccttacgc gatgaccgac gctcgcttgg tctcgaaggc cgggacctcc
      541 gcgcctcggc tggcccagac caggccgcca ccggcgagga tgaaggtcgc gcccactccg
      601 gcgaacaggc cggccagggc gagcccgagc tgcagggcct gcgcggtcac cgtgccgaca
      661 cccagctcac cgatgagggc atgggccacg ccggtccagg cctgtgcgcg gaccgccgcg
      721 tcgatcgggt tgctgcggtc gaaggctgcc cagtagcggc cgtcaaccgg gatctcgtac
      781 ggcgtgccag cggtgtacac cgtgccgtca ggggcggtga agtcctcctc gaggacgacc
      841 gtctgcgtgc cgtgcagggt gtggaaggcc acggtggcca tctggtacat gtactcgctg
      901 gccgtgttca cgaccgggtc gttcgggtcg agctcggagg cgacgaccgg gtagccccag
      961 tcatccttca gcaggcccat gatggcctcg gcgccggcgg tctcgccgcg gtcgaccagc
     1021 tggccatctt cgttgtagct cagggtgacg ttctgcgccg cgctgaactt ctgcagcgac
     1081 gcggtccctt cctggacctt caggtaggcg aatgcaccgg cgccgatgaa ggcgatgccg
     1141 atgagggcga ggacgatgcc cagacccttc agacgattga tcacgacggt gtccctctcg
     1201 tgctaatgct gacggagtag gtttggtttc catggacact gtcgtggttg agccgatgca
     1261 ctcgcgtgtg ccacccggac cgatgtattc cccaagtgac ccgggcaggg ccgggacatc
     1321 gggcccgggc cgttcggccc gccacccgcg gcggtccggc cgagaggacc tccgggcacg
     1381 cgaaagccgg ccccgcgccg agggaacccg cggcgcgtga ccggcccggt gacgcggcga
     1441 cctagatgcc ggtctcgcgc cgctcctgct cgatgacctc gccctcgcgg cgctggtcgc
     1501 cctcgaaggg ggacgtcccg gacgccgcct cgccgcgggt ccaggcatcg ccggcatccg
     1561 cgtcggccgt gctcccggca cccatgggac ccgggcccgc cacggtctcg tacgggtcgt
     1621 cgtagccggc cggcgtgaca cgcggaccgg tgtccgtcgc cgcggtgccc tcgaacgtct
     1681 cctcgtccag gccggcgccg aagtcgtcga cgcgcgtcgc ggccgtgccg aagccggtct
     1741 cgaccggtcc gtcggtggca ccgtagtcgg gtccggtcgg ccggttcgcg gggtcgccga
     1801 ggtcctcctc gaacggctcg ctgaagccgg cctgcggggc cgaggcttcc gggctgaccg
     1861 tggccgtgcc ggtggctgcg gtcgtcggga cgtcgtcgcg gaaatcgtcc gcgggcgaat
     1921 cggtcccgtc gtcacccatc ccgaatgcgt tcttgatctt gtcgatcagg ctctcgtcgc
     1981 ggtggtcgtt cacgtttggt gtgcctccag tggggcgtcg atttgccgcc accgaatgca
     2041 attgccgggc cgtggaccgc acgttcggag cgtcgcgcca ccgattccgc gcgaatggtg
     2101 gtcacaaacg tcttaggtat ggtagtttcc cccgatccgt ccacgggtga cgttgctgga
     2161 ggaaggtaga tgcaactacg gcggcttatg gttcccctgg tgctcctgat gctcatcctc
     2221 gctgcgtgct cgcagaacgc gggttcaagc gccagtgagg agccctcgca ggctccggca
     2281 tcggaggcac ccgcgtccga gggtgccgag tccgaggcgc cggcggcgtc cgaggagccc
     2341 gagcgatccg gctcagcacg catcggtatc ggtggctggt ccatcgccta tgttccgacc
     2401 gcgatcgtga tcgatcgcat gaacgagctg ggttacgaca tcgaggcggt cgagacgagc
     2461 ggcacctccg cccagatgca ggcggctacc cagggtgagc tcgacatcac gtcggtgagc
     2521 gcggcggcga cgatgacggc catcgacgcc ggtctcgact cgaagttctt cctgacgcgc
     2581 aacctcaacg agttcgtgtt cgtggccaag tcggagttcg actcgtgcga gtcgctcgac
     2641 ggccagcgcg tcgccatcca cgcccagacc gatatcaccg gcctgctcac catcaactgg
     2701 ttcgagaaga actgccccga cgcgaacccg cagatccaga tcgtcgaggg ctcggagaat
     2761 cgcctggccg gcctgctgca ggaccagctg gacgcgagcc cggtcgacat cggtgacgcc
     2821 atccagctcg agcgcgagcg gcccggcgag ttcaccgtgc tggcctcgtt cgtggacgag
     2881 ttcccggtcc tgcagggcgt ctacgcggcg agcccgtcgt ggctggccga gaacgagcag
     2941 ctggtgaagg acttcatcca gcttcacctc gaggtgtggg acgagttcta cgccgatccg
     3001 acctccatcc tgcccaaggc cgaggagctc ctgccggaga tcgatcccga ggtgctcgcc
     3061 cagctggcgg aggagtacgc tgacaaggag atcttcccgc cggacggcga cctcgcggag
     3121 gaggagacgc agtacacgct cgacttcttc tctgaggcag ccggcttcga gaacgtggga
     3181 agctacgagg acatcgctga ccgccagtac ctcgacgcgg tgctggaagc gcagtagtcc
     3241 cggcaatccc ggaccgggcg gccgtccatc ggccgcccgg tcccgtcgca ccgcccttca
     3301 ccgattgacc ccaacgcccg accgagaagc agagcctcga tgacgaccct ggtcgctcgc
     3361 ccccggacgt tcgcccgcgt gcagggcggc ctgaagaagg tcctgcttcg ctcggcgatc
     3421 ctcgtcgtgc tgatcgtgct ctggcagatg ggcggcaacg acgaggtccg gctcggcctc
     3481 ccgaccttcg ttggcaccat gcgcgcgctc ggcgagatgc tcgccgacgg ctcgctgctc
     3541 gaggcgctgt ggatcaccaa ccaggcgctg gtcgtcggct acctgctcgc gatcgtcgtc
     3601 agcctgcccc tgggagtggc gatgggctcc agcccggcgt tcgagcgagt cgccggcccg
     3661 tacctgatca tcctgcaggc cgcgccgatg atcgcgctgg tgccgatcgt ccaggccggc
     3721 ctcggcctga ccttcgccgc tcgtgtcgcg atcgtgttcc tcttctcgtt catctacatg
     3781 acgatcaaca ccatggtcgg cgtccgcagc gtcgaccccg cgctgaagga gatgtcgcgc
     3841 agcttcctgg cgagcgcgtc gcagcgcatg cgattcgtca tccttccggc cgccgtgccg
     3901 gggatcatgg ccggcgtccg gctcgggctg ggtcgggcca tgatcggcat ggtcgtcgcc
     3961 gagctcgcgc tcatcggcgc cggggtcggc agcctcatcc tcgactacca ggtccggttc
     4021 cagccggcct acgtgttcgc catcatcctg gtcgtggtgc tggagggcgt gctcctcatg
     4081 gagatcgccc gccggctgga gctgcggctg agccagtgga aggggagtac cgtccttgag
     4141 taagcccgcc atcgagatcc gcggcctcgg gaagatgtac cgcgagtccg acggagacgc
     4201 cgcgttccgc gccctcggcg acgtgaacct caccatcggg gacggcgagt tcgtcagcct
     4261 catcgggccc agcggctgcg gcaagacgac gctgctgaag atcatcgacg ggctcatccc
     4321 gttcgacgag ggcgagctcc tggtcaaggg tcagccgatc cgaggcccgg ggcgcgagcg
     4381 agccgtcgtg ttccagaact tcgctctcct gccgtggctg aacgttcgcg acaacatcgc
     4441 cttcggcctg aagctgcagg gaaccgatgc ggcggccatg cgccagaccg ccgacgagct
     4501 gatctcgatg gtcgggctcg acggcttcgc cgaccgctat ccgcgacagc tgtcgggtgg
     4561 catgcagcag cgcgtcggcc tggcccgcgc gctggcggtg gacccggcca tcctgctcat
     4621 ggacgagccc ttcggcgccc tcgatgcgca gacgcgcaac atcctgcagg gcgacctgct
     4681 ggacatctgg gatcggtcac gcaagacggt cgtcttcgtg acccacgcca tggacgaggc
     4741 tgtcttcctg tcggaccggg tcgtgctgat gggcacgcat cctggccgcg tccacgaggt
     4801 gatcgacatc gatctgccgc ggccgcgcac ggacgagacg cgcagtgacc cgcgcttcgt
     4861 cgagctgtcc tcctacgtgt gggaggtgct gcgcacgatg atcgggcatc cgcgtcgcca
     4921 ggtggccgcg gcttcatgac gcagggcgca gcggtcatcg gtcagccggc accccggcgc
     4981 gcgctggtcc cgcgccgctg gctggcgcac aacgggccgt tcctgctgtc gttcgtgctg
     5041 gtcatggcga cgtgggagct tctcggccgc acgctcgacc tgctgatgct gccgccgatc
     5101 tccgccattt tcgcggcgct gtggcagttc accatcgatg gcacgctggc tgaccagctc
     5161 ttcgtcagcc tgctgaccct cttcatcggg atggtgatct cgatcctgct gggcatcgcg
     5221 atcggggcgg cgatgggcct gtcccgaacc gtcgagtcgg cgctggacgt atacgtgaac
     5281 gcgatgatgg cggcgccgat ggtggccttc gtgccgctct tcatcctgct gttcgggctc
     5341 ggctacccga cgcgcgtcct gacggtgatc atcttcgcca tcttcccgat catcgtgaac
     5401 gcgttcgcgg gcttgcggaa catcgaccgc agcacggtcg agatggcgcg cagcttcggc
     5461 gcgacgaagc ggcagctgct gtggcaggtg cgaatcccgg gcgccttccc gtacctgctg
     5521 gccggcattc ggctcggcac cgcgcgcggc gtcaaaggcc tgatcaacgg cgaggtgctg
     5581 gtgacggtcg tcggcctggg cgggctggtg aaccagtacg gcaccgcctt caccatggac
     5641 cgcctctacg cgatcatctt cttcatcatc gcgctcgcgc tggtgctggt gcgcggcgtg
     5701 gagtggatca gccggcgcct cgtcccgcag gcgtgaaccg gccgtgagcg cctcgtgacc
     5761 cgctacgggt cggacatcgt cgtcgacgtg ctggtcgacc tggggatcgg gcacgtcgca
     5821 ttcaatcccg gcgcgtcgtt tcgcgggctg cacgactcgc tcgtcgatcg cctggacgac
     5881 gggggaccgc ggctgacgat gaccctgcac gaggagatcg ccgtggcggc ggcgcacgga
     5941 tatgccaagg ccaccgggcg gccgatggcg gtggcgctgc acgacgtcgt tggcctgcag
     6001 cacgcatgca tggcgctgtt caacgcctgg tgcgaccgcg tccccatgct cgtcctgggc
     6061 gccaccgggc ccgttgacgc cgcacggcgt cgcccgtgga tcgactggat ccacacggcc
     6121 aacgtgcagg gctcccatgt gcgcgacttc gtgaagtggg acgaccagcc gatgagcgtg
     6181 ccggccgccg tcgagtcggt gcagcgcgcc tacctgctag cgaccacgcc gccgcaggca
     6241 ccgacgtacg tatgcctgcc ggtcgaggtg caggaggatg cggttgaggc tcacgcggag
     6301 ctgccgccgg tgcgccccgt cggccgcgcc gcgcctgacc cgagcgccat cgacgacgtc
     6361 ctgggcctca tcgccgacag ccgacggccg atcatcgtcg cggaccgtgc ggcggaccgg
     6421 cccggagcat tcgaggcgct cggcgagctg gcactcgcca tcggcgcgcc gctgctcgat
     6481 gggggagccc ggttcaacgt ggccacgacc cacccgaacg acctcacggg cgacgagcgc
     6541 caggtgctcg cgtcggcgga cctgctgctc gccttcgagg tcgccgacct ggccgccgtc
     6601 ctcgcggcgt cgcccgagcc cgtgacggca cagatcgtcg acgtcgggct gtcgcacaac
     6661 ctggtcggct cgtgggcggc cgactaccag cgcctggtgc ccgtgcgcgt cgtgctgcca
     6721 gcggacgcgg gcctcaccgc acgggcgctg atcgacgggt gggggcgacg aggccgtgca
     6781 cgagacgccg ccgagggccg caaccgcgaa atcgtcgccc gccaccacgc cctgcgcgag
     6841 gagtggtcag ccgtggccgc cggatcgagc gcgcgcaccc cgatctcgac cgcgtggctc
     6901 gcacacgaga tcggtcgtgc gatcggtgga cgtgacgccg tgctcagcaa cggcacgctg
     6961 aacggatggg cgcgtcgcct gtgggacctc gaccgccccc gcgccatgct gggcgggaac
     7021 ggcggaggag gcatcggcta cgggatcggc gcgagcgtcg gcgctgggct cgcggcacga
     7081 gatgaggggc ggatggtggt caacctccag cccgacggcg acctgctgta cgcgccgtcc
     7141 gccctgtgga ccatggcgca cgaggcgttg ccggtcctga cggtggtgtg gaacaacggc
     7201 ggataccgga actccgagga gcacgccgag cgcgtcgccc tggcgcggtc tcgctcggtc
     7261 gaccgcgcgg gcatcggcaa ccggatcgag gcgccggcgg tcgacatcgc ggtgctggcc
     7321 cgcgcctacg gcatcccggc ggagggcccc gtcgagagtc ccggggacct gcgtgcggca
     7381 ctcggccgag cggtatccgc ggtggcggac ggcggcccgg cgctggtgga ggtccgagcc
     7441 gctgcccgct gacgtgctac gacaggttga ggccgcgcag gatcagcgtg atgcccgcga
     7501 tgccgatgag caccagcgcg ccctggcgcg cgtgcgtcgg cttcacccag tggtgcagcc
     7561 ggaagccgat ctgctggccg atcagggccg gaatgaccag tccgaccccc gtcccgacga
     7621 tggcccacgt cagctgaccg agcgccgcca gcgacacgag gcgcaccaga cccatcgcga
     7681 agaagagcag cgagatcgtg aagccgaagg cggccgggct cagccccagc gcgatgaggt
     7741 agatgcccaa caccggccca ctgaccccga cggtgccgtt cgcgacgccc gccgcaacgc
     7801 cggcgaccgg cgacgcgatg ggctcagccg cggccgggat gcgagggccg cggttggcga
     7861 aggtcgagac cacgaagagg acggccatgg cgcccagcag gagggcaatg gcgtcggcgt
     7921 ggaggacgcc cagcagcact gccccgagcg gcacgccgat gaatccgcca agggcgatgg
     7981 gcgcaagtcg ccgccagccg ggatagctgg tcttgtggtt catcagctgc atggagctga
     8041 tgaacggcgt ggtgacggca atcagcagga cggccgtctt cgggtccacg gccagcgcga
     8101 agccggccgc ggcgatcatg gcgaacccca gcgccgtcca gccgttcacg agaccggccg
     8161 cgaacatgag gacgatcagc gccgggttca gcatggtcta atgacgttgg acgtttgttc
     8221 ggtcgcgcct gcggcgcgag tagccgaggg gaatggaaga tgaccgatgg ggcagcgccg
     8281 catcccgcgc cgagcgtcag cgatgacagc ggcgggcatc gggatgcggg gcgaacgccg
     8341 ttccgggggg agcatctggt ctcgcagtcg gccgcggagg ccatcgccgg ccaggtccgt
     8401 ggcgccctca tcgagggcag gctccagccg ggcgaccggc tcgccaccga gccggagatg
     8461 gccgaggact tcggcgtcag ccggtcgacg gtgcgcgaag ccatccgcat cctgcgcggg
     8521 cagggcttcg tgcagacggt gcgcggggcg aagggcggcc acttcgtggt gacaccgcag
     8581 accgacgtcg tcgcggagtc cgtcggggag acgctgggcc tctggttcga ggccggcacc
     8641 gtctcggtcg ccgaggtcga cgaggcccgc aacctggtcg aggccgcctg cgtccgcctc
     8701 gccgccgagc ggcgtacgga ggaagacctg cgtgcgatgg aggatgtcct ggccgacgcg
     8761 gccgtcgacg accagccgct gcgcgccttc ctcgatcacg atgtcaggtt ccatcgctgc
     8821 atcgcgcgcg ccgcgcgcaa ccgcctgctt gagctgccga tgacggccat ccacttgatc
     8881 cggccgcgga cgaacgtcct gctgcggcga cacgatcgag agacggtgcg cgcccagcat
     8941 cgacagctgt acgaggccat ccgcgacggg gacgccgatg ccgccgaggc ggcgttccgc
     9001 gaccatgcgt cgtacatcgc cgaggagcgg gccgcggccg ccgaggagcg gcagcgccga
     9061 ccgaccgaga tcgcggtgcg ggagctgcct gagctgagcg acggagagca ctcggcgacc
     9121 tagtgcagaa tgccgccgcc ctcgaccacg agggtctggc cggtgacgta agcactgtcc
     9181 tcggagagga agaagacggc ggcgccggtg aggtcgtcgg gcatctcgat ccgcttgatg
     9241 gaccgattcg cgatcggtgc cgagcgcatg gtgatcacgt cgtcggaggg atcggcctcg
     9301 ctgagggtgc tccccggcac gatgacgttg acccggatgt tgtccggcgc cagctcgcgc
     9361 gagaggacgc gcgagaggcc gatgacgccg cccttcgagg cgacgtagtg agcgcgcgtc
     9421 ggactgccca ggaagaacgt tcccgacccg atgttgacga tgctgccgcc gccgcgccgg
     9481 cgcatggcgg ggaccgccgc ccgggttgcg agccacgtgc ccttcaggtt ggtgtcgagg
     9541 acggcgtccc actcgcgctc gtcgatgctc tcgaatccga cgcgcgagat ggggatgatc
     9601 gtgagcatcc cggcgttgtt gatgaggccg tcgacgccct cgaaggcctg ctcggcatgg
     9661 gctacgaccc gctccatcga ctccaggtcg cgcacgtcgg cggccatgcc gaccgcggcc
     9721 ccgccgtcgg cgacgatccc gtcggcgacc tgcctggcag cgtcgccgtc gatatcggcg
     9781 atgacgacgc ttgcgccctc cgcggcacaa cggcgcgcgt aggcgcgacc gatgccgtgg
     9841 gcgcccccgg tgatgacgat gcagcgacca tccaggcgca tgctccggct ccttctcagt
     9901 gcgtgtcgaa gagggtctgg agatcgcggc cgcgtccggc ggccagcgcg acggccgtca
     9961 gcacacgcgc cttctgcggc gtcaggttgt cggcgcggac ccaccacgag ccgtcgacgc
    10021 gagggatgcg cccgccgcgc ccacggctgg cgaagatcac ggggactccg ctccgcgcga
    10081 cgtgctcgag cgtctccatc tgcggatcgc ttccacgccc gctgtacgcg aagccggcca
    10141 ggacgatggc ttcggcaccg gcggccacaa ccgccttaac tcccacggca tccgcgccgg
    10201 cgtaggcgct gacgatgtcg acgcgcggca acgacgcgag cggcgtccgg cgcagctccg
    10261 accggccggt gtgccggcgc agcgacgcgc ggtacagcgt cggctggtcc agctcgatcg
    10321 tgccgagcgg gccgaacggt gctgaagtga accccccggg ccgctggttc tccttcgtca
    10381 cctcgcgggc ggcatgcacc tcctcgttca gcacgaggag gacgccgagt ccccgcgcat
    10441 ccggcgtgag gcacaggcga atcccgtcga ggaggttccg atcgccgtcg ttgcccgcca
    10501 tccggtgctt gcgctgcgag acgaccacga cgaccggctt ggaggagtcc accaccaggt
    10561 tgagcaggta cgccgtctcc tcggcagtga aggtgccgtg ggtgaccagg accgcgtcga
    10621 ccgatgcgtc gtcgcaggcc gcctgcacgg tctccgcgat ggtctgccac tcggcggggc
    10681 cgaacgtctc ggagcccgca cgcaggatgt cgtgcacctc gaggtcgacg tcgggcagcg
    10741 gctcgccgtc gtaggtcgcg atccgctccc agaccgcctc gaccgggacg cgttcggaga
    10801 tgcgattctg gatggtgccg ccggtcgcca cgaccgcgat tcgcggactc acgcctcgag
    10861 cacccgtggc gcctcgggac cgaggtggcg gtcgaaccac gcggtcgcct cgtccaggat
    10921 gagatccatg tgcttgacgt aggtgccgta gtgctccgtg ctgtcgatct cgaccagccg
    10981 cttcgggctt ccggccgcct cgtacgccag ccgtgagtgc tcgaccgggc aggtcgggtc
    11041 cagcgtggcc gcgatgagca gcacggcgcg cggcgcgatc cgcgccacga gatcgagcgg
    11101 ccggaactcg gcgatcgcct ccaggcaggc gagcgggatg ggcggaatgg cctcctggct
    11161 gagggccgag gtcttcgatc gttcgggcgt tggcggagag aagtgctcgg tgacgccgcc
    11221 gctgccggtc gtcacacgac ggcgtcggtc ctcagcgagc tgcgcgagca gctcgaccca
    11281 ctcgtactcg cggcgcgagc cgcgaagcat catcgcgccg tccatggggg agagaacggt
    11341 caccaccgcc cggatgcggg cgtcgtgcgc ggcggccgcg acgcccagtg ctccaccgaa
    11401 gctgacgccg aagacgccga tgcgtccggc atcgatgcgc tgatccgtct cgaggtacgt
    11461 gaccgcgttg cggatgtcgg cgacctgctg ccacgggtag agctgtcccc gtgggccctc
    11521 cgactccccc cacccccggt agtccatcag caggaccgat gtcccggcgc caacgagccg
    11581 cgggatgtac tcgtggtagt acggcgtccg tttgctcacg cgagaaccgg ggcagaagac
    11641 gacggtggga gcgtgggccg cgtcgaccgc caggtgcagc tccgcgtcga ggcggtatcc
    11701 gtcgctgaag aagctcacgg gttccggaga tggcatcggt gctgactccc tgacgctata
    11761 ctcggccgcg ctaaacgttc atacgttagt ctaagccagc caccggtcat cgggagaggg
    11821 aatgacgatc gagtttgggc tcctgcttcc gcacttcagc gattccacca cctgggatcg
    11881 gctcttcgga tttgctccgc gagccgagga gctgggctac gagtcgctgt gggcccgcga
    11941 caacctgtcg ttccacgggc acggcttcga gcttcccggc gaccacttcg tcgacccctt
    12001 cacgacgctt gccggcgtcg cggcacgaac ggagcgcgcc aagatcggga ccgccgtcct
    12061 gacgccattc cgccatccgc tcatcaccgc gcagctggtc ggcggcctcg actacctgtc
    12121 gaagggccga ttcgtgctcg gcatcggccc gggcacaccg cgcaagccct gggaggcgct
    12181 cggtcggcag cacgaggagc gcgtcgttgc caccaaggag accgtcgagg tcctgcgcct
    12241 cgtgtcatcc ggcgaggacg tctcgtacga gggtgagcag ttcagcttca ccgacgtcaa
    12301 gatcgatcca cccccgagcc ccgacatgat ggtgtggtac ggcggcgcct cgaacgcctc
    12361 gatccgcgcc atcctggact actgcgacgg gatgcttccg ggtcggtgcc cgttcacgcg
    12421 ctacgacgtc gccgcggagc gcctcaaggc tggcgccgcc gagatcggca agcaggtgca
    12481 cctcggctcg attccgaccg tcagcatcgc gccgactcgc caggaggcga tcgacaagat
    12541 cccgatgcag gccctgctgg acaccgcctc cgagcgctgg aagcgtccgt tcgagaagcc
    12601 cgaggacctc gcgggcgccg tcgttgtcgg ctcaccgagc gactgcatcg agcaggtgca
    12661 ggccttcgtc gaccggggta tcgagctgtt cgtcgtcgac ctgcgcctgc tgatgaacga
    12721 gttcgaggaa gccgcggagc tgttcgcggc ggaggtcatc ccggccttcc gcagcgccag
    12781 ctgaggaccg atggtggcgg aaatcccggt ctacaacgcg tcgacaggcg aggtgctgca
    12841 gcacgtgccg gaggctggcg gcgaaggcgt ggccgccgcg gtcgagcgtg cgcgcagcgc
    12901 gagccgagag tggcgctcga tcggcgcggt ggcacgcggc caggcggtcg ccgacttcgg
    12961 ccgagccgtg gccgaccacg ccgaggagct cgcggggatc gatgcgcgca atgcagggaa
    13021 tcccatcacc ggcatgcggc ggggtgccca gcagggcgct cgcacgctgg agtacttcgc
    13081 ggggctggcg ctcgagctca ccggcgaaac gattcccgca acgccggacc acctccacta
    13141 cacgctgcgg gagccattcg gcgtggtcgg ggtgatcacc ccgttcaacc atcccacgct
    13201 gttcgcgacc gcccgaacgg ccgctccgct cgtggccggc aatgcggtcg tcctgaagcc
    13261 ggcgcaccag acgccgctct ctgcgctacg gctccaggag ctggccgatg agcacctgcc
    13321 gccgggtgtc ttcaccgtcg tgaccggtgg cggcgccacc gggtcggcgc tggtgcggca
    13381 ccccgaggtg cggcgcatcg gcttcaccgg cggcgtcgag acggctcgcc ggatcgcctc
    13441 cgatgccgcc gacagcggca ccatcaagcg catcagctac gagctgggcg gcaagaacgc
    13501 catggtgatc ctccccgacg tcgacatcga cgtggccgca gcggccgcgg tggaggggat
    13561 gaacctgacg cgcgtggcgg gccagtcatg tggctcgacg tcgcggctct tcgtgcactc
    13621 gtcgatctgt gacgacctgc gggaccgtgt cgtcaagcgc ctcgagcagc tgcggttcgg
    13681 gccgccggag gaggagagca cgcagatcgg ctcgctggtc tcggagcagc aacgcgagcg
    13741 cgtggaacgg tacgtgcgcg ccggccgcga ggaaggcgcg cagctggtgc tcggtgggga
    13801 gcggccgacg gagccgccgt tcgaccgggg cttctacttt ccgccgacgc tgttcgagcg
    13861 ggtgcgctcg tcgatgacga tcgcgcgcga ggagatcttc ggtcccgtcc tctgcatgat
    13921 cagctggacc gatgaggcgg agatgctggc ggcggtcaac gacagccggt acggcctcac
    13981 ggcgagcgtc tacaccaacg acttgcgtgc ggcgcatcgg ctcgccggcg cgatcgaggc
    14041 cggctacatc tggatcaacg acgtggagaa gcgctggatc ggtgtgccct tcggcggcca
    14101 gaaggactcc ggaacgggga ccgagtactc catcgaggag ctgtacagct tcacccagaa
    14161 caagtccatc agcgtgagcc tccgatgagc gagcgcgtcg cgagggtcat ccgggccgac
    14221 gagcggcggc cgtccgagct gcgctacggg cggggcagcg tcgagaagat cgtcgacggc
    14281 gccatcggtg cggatcgcgt cgaccttcac ctcaaccgca tccgggcggg cagcgcgccc
    14341 ggcccgtacc acctgcacac cgaggcggag aacgtgtacc tggtgctcgc cggcgacgtc
    14401 gtcgtgagga tcgacggcgt cgaccatcgg ctcggcgccg gggatgcggc cttcatccca
    14461 cccggagttc cgcactcggc gtcgaacgtg gggaccacgg acgccgagct gatcgagatc
    14521 tacgcgccgg ccgccgtcga cttcgtcgag gtggcggatc ccggcgccgt gacgcccacc
    14581 gaggagagga gaccgaccaa tggctgacca cgcaacgagg atcgcgggag gggacgtgct
    14641 cgtgcgcggt cagctcgtgc gtgcggacgt gctgatcgat gacgggaaga tcgtggccgt
    14701 cgtggccccg gacgtcagcg cagaggccgc ggagacgatc gatgccaccg gccgcgtcgt
    14761 cctgccgggg atcatcgaca cgcatgcgca cacccgcgag ccgggctaca cgcacaagga
    14821 ggacttcctc acggcctcgc aggccgcggc cgccggcggc gtgacgacca tggtggacat
    14881 gccgaacgtc gagccgccca ccgacacggt cgagcgtctg ctggagaagc gcgagctcgc
    14941 cgatcggaag tcgatcatcg actggggcca cttcgcggcg ggtaccaagc tcgaggagat
    15001 ccctcgtctg gccgaggccg gagcgaccgg cttcaaggtc ttccaggtat cgggcgaata
    15061 cccgcacgac ccgcggctgg ccatgaacga cgagggccag ctgctgagcg ccatgcgagc
    15121 cgtggccgag acggggctcc cgctggtgat ccaccccttc aaccagtcgc tgttcgagaa
    15181 gctcaccgag gaggcgttcg ccgccggcaa gccgaacaac tggtacacct tcggcgaggt
    15241 ctacaccacg gaggcgatct ggcacacggc ggtcaacacg ctgatgaacc tgcaggcgct
    15301 gactggcgcc cgggtccacc tggtccacac gcattccggc ggcagcctgc gcctgatccg
    15361 ggaggccaag gagcgcgggc agcgcgtcag ctgctcggtc gaccccaagt actaccacct
    15421 gacgcgggat gacctgatgc ggctgaaggg ccgggcgtgc cccggcggct tcatcaccga
    15481 ggatgccgac cgaatggccg agatctggcg gtcgctccgc gacggcacga tcgacagcat
    15541 cgacgccgat cacgcgcccc acacgctcga ggagctcgac gtgatggagg aggacgcgtg
    15601 gcacgccgcc atgggctcgc cgcagtacga ctggatgtac agcatcaccc tgaccgatgt
    15661 ccacgccggc cacttcagcc tggcgcgcgc cgtcgagatg ctcgccgagg cccccgcgcg
    15721 catgctggga ctcttcccga agaagggcgc catcctgccc ggatccgatg cggacctggt
    15781 cctcgtcgat ctcgaccgtg aggtcaccct gagcgatgag ggcctgtaca ccaaggtcgg
    15841 ctggacgccg tacctcggcc gcaccatcaa gggcgttgtg acgatgacga tgctgcgtgg
    15901 gtgcgtcatc gcccgtgatc gccaggtcat cggcacgccg ggcttcggtc agtacatcgc
    15961 cggcgtggcg cagtagccgt cgcgccccgt gacggagcga ccgaccgtcg tcgtcacggg
    16021 gccgtggtcc gaccccggct gggacgccgc gctgcgtgcc gccggggtcg gcgtcatcat
    16081 ggggccctcg atcgacgaac ggccggcgac gccggtggac gaggcgacgc tgtgcgagat
    16141 ggtcgcccgc gccgatgccg tcctcgtgtc gtcacgcgag cgcatcacgg cccgcgtcct
    16201 tgatgcggcc ccacggctgc gcctcgtggc caaggccacc atcggcgtgg agcggatcga
    16261 cgtcgaggcg gccaccgagc gcggcatcct ggttgtcaac agcccggcgc ccgagaacgt
    16321 cgtcgggctg gccgaggcca ccatcgggct gatcgtcgcg ctggccaagc agctgctggt
    16381 caaggagcag cggatccggg acggtggctg gcgcgacgcc accaccgacg gcctcgttct
    16441 cgccgggcgg acggtgggca tcatcggcct cgggcgcgtc gggagtggag tcgcgcgacg
    16501 gctcgtcggg tgggacgtcg acgtcctcgc ctgcgatccg tacatccccg tcgagcgctt
    16561 tgcggcggcc ggcgccgggc cggttgacct cccgacgctg ctctcccaat cggacatcgt
    16621 cacgcttcat gtgccgctga ctgccgaaac gcgcggcatg atcgacgccg aggcgctggc
    16681 cgccatgccc gtcggtgcgc tcctggtcaa cacgtcgcgg ggcgcggtcg tggaccaggc
    16741 ggcgatcgca caagcgctgc aggacgggca cctgggtgcc gcggcgctcg acgtgttcga
    16801 gaccgagccg ctgccaccgg acgacccgat ccgcgccgta cctcgcgatc gcgtgctgct
    16861 gacgccacac tccatcggct cgagccacgc gtcccgcgcg accgggacgc gcatggcgat
    16921 cgcggccgtc ctcgacgcgc tcggcggccg cgtcccaccg aacgtcgtga acgccgcagc
    16981 cctgcacagg tggcgtcggc gattcggcgc cgagtgaccc gcggccctcg gacgagcgac
    17041 aaacggaccc gcgggccggt ccgttcggaa ctcgaagcgc ggcgccgcgg ttcgcaggat
    17101 tggagcaccc atagcgacca ccgaggagtg cgaccgatca tgagcctgca acggatcaag
    17161 aacctgtgcg tggcggatct catgaccatc gacccgatcg tcatcgggcc gaaggcatcg
    17221 gtcagcgagg ccgaggagct gatggcggcc aagggcgtca gcgggctgcc cgtggtggat
    17281 gaccacggtc ggctcatcgg ggtcatcagc cagacggacc tgctgtacct cacgcgtccg
    17341 gcggtgcgcg ccctgatgac gcgatgggag acgccgctca cggtggcgga ggtgatgacc
    17401 agtccgccgg tcaccgttcg cacgaccatt tcgctcgaga acgcggcgcg tgtcatgagc
    17461 gagcacgacg tgcatcgcgt ggtggcggtc gaccgcaagg gtcgcccggt cggcgttctc
    17521 tcggcatccg acttcgtcca gctggcggcg atcggctgct gaccgatgcg cgcctccggc
    17581 gcgctgatgg cgctcgccgc gaacgatgac cgcggcgtag gatgcggcgc accacggagg
    17641 gaggggaacg ccgatgagcg ccgagcgctg gatctgccag gtgtgctgga cgtcgaaccg
    17701 tccacgcgac gaggtgtgct ggcgatgcaa ggcgccgagc ggcctcgctc gcgaggaggc
    17761 cgacgcacaa cgcgcggccc gcagcgcccg tggtgaagcg cccgagcccg ttcccgacct
    17821 gttggtggcc ctccccgtcg tcgtgttccg aacctacgcc cgcgtgtggc tgcgcggcgg
    17881 catcggcatg ctggccgtcc cgttgctgat gggcttggcc ggcgtcgtgg acgtcgggtg
    17941 gctggtgctc agcatcggcc tggcggtcgg gctggtcgtc ttcggcatcg tcgccggcga
    18001 ggtgagcgac gggatgcgcg aacgcgaggt gtgggccttc ctcaccggca tcgtgctggc
    18061 ggtcgtcggc gtcatcggct cgatcctggc gttcgccacc ttcgcgccgg agctcatcag
    18121 cccgacggcc gtgcgctggg gaagcctcct ggtgttcggc ggtgccggcg tggcggccgt
    18181 cgccggcctc gtgctgctcg tcagggccga ccgccgtccg accgacgtcg gtcccggcca
    18241 gccgggtccc ggggcgtccg gcgcatgagc cagccgtgga tgtccgtccg cggcagcacc
    18301 atctcctcca cgacgtcgaa tcccaggcgc tcgtagagca gcacgttccg cgcgaccgcc
    18361 gtctcgaggt aggccgggat ccgctccgcg tcgcagcgct ccagcaccgg cgcgagcagc
    18421 gcggtgccca ggccctcacc ctgccgatcg gggtcgacgc cgaggacgga caggtagaag
    18481 tgagggccgg gcgcgtggtg gcgccggcga cgctccaggg cgtcggtcgc ggagctcacc
    18541 gcgcggagcc gccgccagcc ggcgagccga gccatggacg gcagcagccg gaagccctgg
    18601 aggacgcttg ggccccgatc gcccggcggc agccagaccg ccacgccggc gcggtcgtcg
    18661 gcggtccagg tctcgcgcag cccggctgac gcgaaccgca ggatcccgag ccagccctcg
    18721 tacatccgcg cgtggcgttc cggggcgtcg ccggccagga agcgatagta cggatcgccg
    18781 tcgaaggcgc gcgagagaac gctggcgagg gccgagatgt ccgccggcgt cgctcgtcga
    18841 acctgcggtt cggcagccgg cgctggttgc gtggtggcca tcggtccttc gtttatcgcc
    18901 gcgctgtcaa ccgctccaga ggtggattct gcttgacgct tccaccgacg ggacggatgc
    18961 tgcccgtcca cgtggacccg atgcgggagg cgaacgccca gcgcgaccgg ctcgtgacgc
    19021 cggcctttgt cgtgctcgcc gtcgccgagc tcgcctactt cctggcgtcc ggcatggcga
    19081 tcccgctgac gccgcgcttc gccaccggtc cactcggcgc ggacgcgctc ggatcgggca
    19141 tcgcctacgg cgcgttcggc gtcaccgcgc tcctgctccg gccgttcgcc ggacggctca
    19201 gcgatcggta cggtcgtcgc cccttcctga tcggcggcgc cgtgctgtgc agcgtggcgt
    19261 tcgcggccca cgcggtggcc ccgaccctgg gcgtgctggt cgtgctgcgc ctggtgctcg
    19321 gcgtcgcgga ggcgttcttc ttcgtcgccg gcttcgcgat gctcgccgac ctggcgcccc
    19381 ctcgccggat cggcgaggcg ttgagctaca actcgctcgc gctgtacctg ggcgtggcct
    19441 tcggaccggc tgccggcgag gcgctcctgg acattggcgg tttccggctg gcgtggctcg
    19501 gggcggcggc gctcgcggcg gtggccacga tcctggcgtt ggtactcccc gaacggcacg
    19561 acgcgccgag agccgggcaa ccgctcccgc cgctgctgca ccgcggggcc atcggtccgt
    19621 cgatggcgct cttcgtcggc gtggcgggca tggccgcttt cttcgcctcg ctgtccctct
    19681 acgtcccccg cgtcgggctg ggcggtgctg ggccggtgct gctcgtgttc gggctcgtcg
    19741 tcgtcggctg ccgcatcgcc ttcgcccggc tgcccgaccg actgccgccc tacgtgctcg
    19801 ggacggccgc attggtcggc atcggtgctg gattgctggt catgagcgtg gtggccacgg
    19861 catcgggcct gttcgccgga gcggccctgg cggcggccgg cgtcgccttc atcaccccgg
    19921 ccatgttccg cgccgtcgtg gagcgagcga gcctgagcga gcggggcgcc gcactcggaa
    19981 cgacgtcggc attcatcgac ctcggcttcg cctcaccgat gctgttcggc ctggtggcgg
    20041 ccgccggcgg accggccgcg gccttcgccg cggctgcggg cctcgccctg gccggtgcag
    20101 cagggaccgc ccgcgccgca ttcgccgacc ggcggcccat gcagccggcg gtcgcggatc
    20161 cggatcagta gccccgcgga tcggtcgagc cggtcatctc ctcgtcctcg tcggtcggca
    20221 catccgtccc gatgccgccg gtcgcggtga tcgaaccggc gccgaacgag cggtgggtcg
    20281 ccgcgatgcc ggacgcgccc tcgacggcgg tcttctcgtc gccgccggcc tccggctccc
    20341 ggctctcctc gtcgccgctc tccatggcgg gcaggtcgtt gggtcgctcc atgccggtgc
    20401 cgtcggccag cggcgtatcc tcagcgtcct ccgcgccctc gtggcgttcg cgatccatgg
    20461 gtctggtcct cgtgtggcgc ttgtcgccac cacgccgatg caggaacggg gccgccgcgt
    20521 gcgctggcta ctcgcccagc tcgatcggtc gttcctcgcg ctcgccggcc ccgtcgtgcg
    20581 ggacctcgac caggcgccac cccggcaggg gagcgtcgac ctcgccgccg cgctgcgtga
    20641 cgatggcgag cacccgcgcg accagcgctc gggcctcggg ccgcgtggcc tccggccagt
    20701 cgtcgaccag ccgggccacc tcgtcctcga ccagccgggc agcgaccacc gggaagaacc
    20761 cctcgcggtc ctggcgcacc aggccatcgg cgtccgtgaa tccgcgccga tcgagggccc
    20821 ggatcagggc gcgcgcgcgt cgcgcatcgg ccgtcgccag gtcgtccagc caggtgcgcg
    20881 gcttgcggcg ggcgggcggt gcgtcagcct cgtcgccctt gcgctgacgt ggccgcggcc
    20941 ggggacggcg cagggcggtg accttggggc tgcgggcccc gggaaccgcc tcccgcgcgg
    21001 ccttgagcgt cgcgaacggc ccgtgcatca gctcctgccc gaactcgttg gtctgctccg
    21061 agtcgaccac gaaccagccc gtatcgccgc tgcgcacctc gaaacggtcg tcctcggtgc
    21121 ggtacgtccc agcggactgc ctgacgaggc gatcgggatc cggcggaggc tccgccgtgc
    21181 gcttcggcat ggtcccattg tgcgccacgg gccgccgaag ccgactgggc ggagatcgtg
    21241 cgccgcaatc tcccccagcc ggacgagcgg gcactggatg ctggcggccg aacgctgcga
    21301 gcgccggtca ccggcgccag ccgacgaggt gctcgaccgt tcgcagcccg accgactcat
    21361 acagggcgtg ggcacccggc ccgtacgagc cgacgtacgc acgcgtcgcg cccatccatc
    21421 gtgcccgcgc catcccttcc agcagcagcg cgcgccccag gccccgccgc cggtgcggcg
    21481 catcggtccc cgctggctcg atggtggcgg tccgcaggac gtcgtcgaac cagacggtga
    21541 cgaacgcggc cagcgacccg tcatctgcct ccgcgacgag gtcgaggtca cgacggtaga
    21601 gcgggcagcg ctggatgttg tggtagtcct cgccggtcat ggcggtgttc ccgtccggga
    21661 ccggatggaa gacgcgcagc gagatgtcgc cgcgggctgg gaagtcctcg acgccttcgg
    21721 cgagcgcccg gatgcggtag ccgggcgcga gctccggcgc ggggagggga tcgcgcaggg
    21781 accgcatgcg cacgtgctcg tcctcgctcg tgcgtgcgta gccgcggcgg cacagcaacg
    21841 cggcccagcg ttcgtcggac gccggcgtcc agacgagcag gccatccccg ttcgccaggc
    21901 gctcctcggc ggcctcgagc atggcggcct ccagccaggc gctccgacat cgcgggtcga
    21961 gctgcaggaa cgcctccccg cggtcctcgg cgttgacgac gccgatgagg tcgtcggcct
    22021 cgtcctcgac gagcgtcacg agctcctcgg gaggtggtga gccgcagttc aggacgccgt
    22081 gccatcgcca gtagtcccag cgcggcacgc cccacgtggc gggatggaag tcggcctcgg
    22141 ccaggaggcg gcggaacagg cggcggactg accagaagtc gtcgtcgccg gcgtacgtgc
    22201 gcagtcgcgg cagcatgagg gcaggctcgg cgctccgtgc cggcgcgaac agggccacgt
    22261 ggcctccgcg gagcgtgacg tcgttccggg ccgtccggcg gctgcggctc ctgcgcctcc
    22321 gaacccggga tcggacgcgc gcgacgtaaa cttgcgggcc atggcactgt accggctcga
    22381 cgaggacccg cagctcgacg cccccgtcct ggtggcggcc ttcgagggct gggtcgacgc
    22441 cgggacggcg ggaacgacgg ccgctgcgca gctggcgaac gacggccggc aggtggccag
    22501 cttcgacagc gacgccatct acgactaccg ggcgcgccga cccacgctcg acatcgtcga
    22561 cggacggccg gtgcgcctcg agtggccgga gctccgcctg accgccacgc gcatcgggga
    22621 gcgcgatctg ctggtcctgg cgggtcccga gcccgactac cgatggcgcg agctcggcga
    22681 cgaggtggtg cggctcgcca agcgcctggg cgtggcgacg tgggtgtcgc tcggcgccat
    22741 cccggccgcg gtcccgcaca cgcggccggt gccgatcctg gcgacggcat cggcccccgg
    22801 cctgctgccc gagggcatcc gccagggacc tgacgggctc ctgcgcgtgc cgtcggccgc
    22861 cctctcgatg ctcgagatga gcgcggcgag caacgggatc ccggccatcg gcttcttcgc
    22921 ccaggtgccg cactacgtca acgcgacgta cccggcggcg tccatcgcgc tgctcaacgc
    22981 gctcggccgg tacctcggcg cggaccttcc ggtcggcgcc ctggccagcc gtgccctgga
    23041 gcggcgcaac ctgctcgatg ccgcggccgc gaccgacgac gacaccaagg cgtacgtcac
    23101 ccgactggag gcgctcgccg acgagtcacg gctgcccgag ggcgacgagc tgatcgccga
    23161 catcgagcgc ttcctgcgcg agggcgggga ggaaggcgga gacggcggcg attccggtgg
    23221 gggctcgcgc ctccactgat cacgcgccgg acgggcgctc cgcgcgcagg agccaggtct
    23281 cgacgacgcc cttgcccttg acctcgatcg gcccgtgggg ctcgcacacg aagcggttcg
    23341 ccagcagctc gtacatcgcg cgcgtgagca tgatctcgtt cggaatgccg tgggactcca
    23401 tccgcgacgc cgtgttcacg gcgtcacccc acaggtcgta gaggaaccgg cgacgaccga
    23461 tgacgccggc caccaccggg ccggcattga ggccgatgcg aatcctcagc ggccgcccgt
    23521 cgtccgcccg cgggaatcgc ccgacacact cacgcatgga cagcgc
//
LOCUS       DASTYP010000011         6595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_37114, whole genome shotgun sequence.
ACCESSION   DASTYP010000011 DASTYP010000000
VERSION     DASTYP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37114"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFV59_00580"
     CDS             <1..865
                     /locus_tag="VFV59_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEU5324361.1"
                     /translation="LVENETQIEYPVVQLGEHPQTGAPVVVRIGRFGPYVQVGEGDDA
                     INASVPDDVAPADFTLQDAIALVESRAKGPASLGEDPKTKLPVYVMTGRFGPYVQLGE
                     TPEKGSGEKPRRASLTAADDPDQLTLERALELLSLPRVLGRDPETGEEVVANFGRFGP
                     YVKRASEFRSLASDDEVFSVTLDQALELLRQEKPTRRGASRKVLRELGAHPGSGAAVR
                     LLEGRYGPYVTDGATNASLPKDANAEAVTLDDAVALLKAREGQPSRGRGGRSGASRGT
                     SRRAPARRPRG"
     gene            900..1862
                     /locus_tag="VFV59_00585"
     CDS             900..1862
                     /locus_tag="VFV59_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEU5324362.1"
                     /translation="MTPAIGPPLPSAAADALDQLLTGLRVRNASVGTLREYRRHAGEF
                     LAFLAGRGIDWRRPDRAAIRSYLASLADRGLAPSSVAGRLAAVRALYRHALRNGRIDA
                     DPLVGVRAPRRPARLPRVLSVDEAAALVTAPRRGGRQEEALTLRDAALLELLYATGMR
                     ISELAGLSMDRLDVDRRRLRAFGKGSKERDLVFGAPAASALRAYLAQARPVLAGRSAP
                     SAAVFLNARGAPLSARGARLVIERWVGASGMPARTSPHTLRHSFATHMLEGGADLRVV
                     QELLGHANLQTTQIYTHLSDAALRSAYREAHPRAGRARDGRGGR"
     gene            1859..3478
                     /gene="murJ"
                     /locus_tag="VFV59_00590"
     CDS             1859..3478
                     /gene="murJ"
                     /locus_tag="VFV59_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU5324363.1"
                     /translation="MSDAPAPPAPDSTTPRPGSTGRVLLTASLILTVAALASRLLGWV
                     RLVVIGSQFGASREVDAYFAAFRIPDAIFQLVVAGALSAALIPVFQSYRARGQDAEAW
                     ELASSVINLVLIGLAGLSLLMVIFAPVFVPIVAPGFDAPTTELTIRMTRVMLLSPVLI
                     GMGAVVTGILNSYQQFTVPAIAPLLYNVAIILAAILLAPIMGVEGLAVGVAVGSLAHL
                     VVQLPNLARVGQRYDLTVSLRHPGVRRVAWLMGPRTLGLAAGQLNFLVSTVLASGLPE
                     GSLTAYNYAFQLSQLPVGVVGVSIAVALFPTMSQDAALGRIGEIRRQVANAIRVLVFV
                     AAPLTAIMVVLREPLTSVFYQYGLFGQSATDRTASTLLFFSLGLVGHIVVHVLTRAFY
                     AMQDTKTPVLWAIVAVAINVPLMAALVGPMGVEGLALALSISAVLEVIGLLWSLHRRI
                     ESVEEATIGRSVARAFVAGVAAALLMLGGLTVVEGSLAGWLDNPVGRLLALLVLSSAG
                     IAIFLIVAAALRAPELDQLRSLLRRRTRRRA"
     gene            3475..4554
                     /locus_tag="VFV59_00595"
     CDS             3475..4554
                     /locus_tag="VFV59_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEU5324364.1"
                     /translation="MIDFRPATDADATTWQSLLEATATGDFLHDWAWAEVAAFDGQPQ
                     RRYVIEQAGEVVGLAAAQERPLVAGRSFWYVPHGPVLDCAHPDAAARLRAMLLGLRAA
                     ARHHRAVAVKLEPRLAADDPGVALFARLEGRSLRRTDEKLQVGQTRTVRLADDDALIG
                     GFDKDTRYGVRRAEREGVVVRVVEDATDASAIDALHELVGETQRRAGFPMPSRERYRI
                     AWRSLAGAGRAAILEASREGVLLASGMVVVEGDRSFYLFSGSRREEPGEPKRYASYAL
                     QWEMMRYARGRGVRLHDLWGIAPAGAGSDHPWHGVGLFKKGFGGSEVEWAGSWDVVAD
                     STLYRVRAALGVARGWLRGLRRLRP"
     gene            4551..6050
                     /locus_tag="VFV59_00600"
     CDS             4551..6050
                     /locus_tag="VFV59_00600"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5324365.1"
                     /translation="MTQPTPHQMGLGALTDAIAPEQVIGIPVGSVTALAYDSRRVTPG
                     TVFFAVPGVHVDGHDYALDAARAGAIAAVVERPIDGLGIPQLVVDRSRRALADAADAW
                     FGHPSKRLEVIGITGTDGKTTTAFLAAEVLRAGGRRPGLIGTVAVDVGDERRSNDERT
                     TTPEALELQALLADMVAAGNDSVVMEATSHGLALERTRNCRFDVGVVTTVTSEHLEFH
                     GSVEAYRAAKARLVEEAPLAILNADDGTFTYYRDRARNRLLTYAIDADADLRATDVRA
                     DASGSRFRLQAPGWSGDVHLPMPGRFNVSNALAALAVAHAEQVPLDAAAGALERTAGV
                     PGRMERIDAGQPFAVVVDYAHTADSLAKVLRMLRPVTTGRLVAVFGSAGERDPTKREP
                     MGRVAAELADLVVVTDEDPRLEDPRVINEQIADGARAAGAREGERLWVIDDRREAIGR
                     AIGMARPGDVVLLAGKGHEASIFYGTEKRPWDDRSAAREALAAAGWGTA"
     gene            6047..>6595
                     /locus_tag="VFV59_00605"
     CDS             6047..>6595
                     /locus_tag="VFV59_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEU5324366.1"
                     /translation="MTSTDLRAFRVTDAARWNAFVESAPYHAFPQLWEWGEVRAMGGW
                     RPIRLAVGPSQDEPVAGVQLLLRPVPLVDWHLAYAPRGPIGRLDDPETRVALVKALRV
                     LGREEKIATVRADPEATRQTHYGQALLSVPWRAAPKVQPPTTRVVDLTVGEEELRSNL
                     KRKHRQYVNKAERAGVTVERFDG"
BASE COUNT          843 a         2392 c         2415 g          945 t
ORIGIN      
        1 gctggtcgag aacgagaccc agatcgagta cccggtcgtg cagctcgggg agcatcccca
       61 gacgggcgcg ccggtggtcg tgcgcatcgg tcgattcgga ccgtacgtgc aggtcggcga
      121 gggcgacgac gccatcaacg cttccgttcc cgatgacgtc gcgccggccg acttcacgct
      181 gcaggacgcg attgcgctgg tcgagtcccg cgccaagggt cccgcgtcgc tcggcgagga
      241 tccgaaaacc aagctgcccg tgtacgtcat gaccggccgg ttcggtccgt acgtccagct
      301 gggcgagacg ccggagaagg gcagcggcga gaagccccgc cgcgcgtcgc tgacggccgc
      361 cgacgatccc gaccagctga cgctggagcg cgcgctcgag ctgctctcgc tgccgcgcgt
      421 cctcggccga gatccggaga ccggcgagga ggtcgtggcg aacttcgggc gcttcggacc
      481 gtacgtcaag cgcgcctcgg agttccggtc actggcgtcg gacgacgagg tcttcagcgt
      541 cacgctcgat caggcgctcg agctcctgcg ccaggagaag ccaacccgtc gcggggccag
      601 tcgcaaggtc ctgcgggagc tcggggcgca tcccggctcc ggagccgccg tccggcttct
      661 cgaaggccga tacggtccgt acgtgaccga tggcgccacg aacgcctcgc tgcccaagga
      721 cgccaacgcg gaggctgtca ccctcgacga cgcggtggcg ctcctgaagg cgcgtgaggg
      781 ccagccgtcg cgcggccgtg gagggcgcag cggcgcctcg cgcgggacct cgcgccgggc
      841 gcccgccaga cgcccgcgtg gctgacgacc ggcccggtgt ggacgacgca ctcggtgcgg
      901 tgacaccggc gatcggcccc ccgctgcctt ctgcggcggc cgacgccctg gatcagctgc
      961 tcaccggtct ccgcgtgcgc aacgcatcgg tggggacgct gcgcgagtac cggcgccacg
     1021 ccggcgagtt cctggcgttc ctcgcggggc ggggcatcga ctggcgccgt ccggaccgag
     1081 ccgcgatccg atcctacctg gcatccctgg cggaccgcgg gctcgcgccc agctcggtgg
     1141 ccggtcgcct cgcggctgtc cgggcgctct accggcacgc cctgcgcaac gggcgcatcg
     1201 acgccgatcc gctcgtcggc gtgcgtgcgc cccggcgacc ggcgcgcctg ccccgcgtgc
     1261 tgtcggtcga cgaggctgcg gcgctcgtga ccgccccccg gcgtggcggt cgccaggagg
     1321 aggcgctcac gctgcgcgac gccgcgctgc tggagctgtt gtacgcgacc ggcatgcgca
     1381 tcagcgagct cgccggcctc tccatggacc ggctcgacgt cgaccgccgt cgcctccgcg
     1441 cgttcggcaa gggcagcaag gagcgtgacc tcgtcttcgg cgcgccggcg gcctcggcgc
     1501 tgcgcgcgta cctggcccag gcgcgtcccg ttcttgcggg gcgttcggcg ccgtcggccg
     1561 cagtcttcct caacgcgcgc ggcgcgccgt tgagtgcgcg cggcgcccgg ctcgtgatcg
     1621 agcgctgggt cggcgcttcc ggcatgcccg ctcgcaccag tccgcacacc ctgcggcact
     1681 cgttcgccac gcacatgctc gagggcggcg ccgacctgcg ggtcgtgcag gagctcctgg
     1741 gtcacgcgaa cctccagacg acccagatct acacccacct ctccgacgcg gctctgcgca
     1801 gcgcctaccg cgaggcgcac ccacgcgccg ggcgcgcgcg tgacggtcgg ggcggccggt
     1861 gagcgacgcg ccggcgccgc ccgctcctga ttcgacgacg cctcgtcccg ggagcacggg
     1921 tcgcgtcctc ctcaccgcca gcctgatcct gaccgtcgcg gcgctcgcga gccgcctgct
     1981 cggctgggtt cgcctggtgg tcatcggctc gcagttcggc gccagccgcg aggtggacgc
     2041 gtacttcgcg gccttccgca taccggacgc catcttccag ctggttgtcg ccggcgccct
     2101 gagcgcggcc ctgatcccgg tgttccagag ctaccgcgcc cggggacagg acgcggaggc
     2161 ctgggagctc gccagcagcg tcatcaacct ggtcctcatc ggcctggccg gcctgagcct
     2221 gctgatggtg atcttcgcgc cggtcttcgt gcccatcgtc gcgcccggat tcgacgcgcc
     2281 gaccaccgag ctgaccatcc gcatgacacg ggtcatgctg ctgagcccgg tgctcatcgg
     2341 catgggggcg gtcgtcacgg ggatcctcaa cagctaccag cagttcaccg tgcccgccat
     2401 cgcgccgctg ctctacaacg tcgcgatcat cctcgccgcc atcctgctcg cgccgatcat
     2461 gggcgtcgag ggcctggcgg tgggggtggc ggtcggctcg ctggcgcacc tggtcgtgca
     2521 gctgccgaac ctggcgcgcg tcgggcagcg ctacgacctg accgtcagcc tgcgacatcc
     2581 cggggtgcgt cgggtggcgt ggctgatggg cccgcgcacc ctgggcctcg ccgcgggcca
     2641 gctcaacttc ctggtcagca ccgtcctcgc ctccggcctc cccgaaggca gcctcacggc
     2701 ctacaactac gcgttccagc tgtcgcagct acccgttggc gtggtggggg tcagcatcgc
     2761 ggtcgccctg ttcccgacga tgagccagga tgccgcgttg gggcgcatcg gtgagatccg
     2821 gcgacaggtc gcgaatgcca tccgggtgct cgtcttcgtc gccgccccgc tgaccgcgat
     2881 catggtcgtg ctgcgcgagc cgttgacctc ggtcttctac cagtacggcc tcttcggcca
     2941 gagcgcgacc gaccgcaccg cctcgacgct gctgttcttc tcgctgggcc tggtcgggca
     3001 catcgtggtg catgtgctga cccgcgcctt ctacgccatg caggacacga agacgccggt
     3061 cctatgggcg atcgtcgcgg tggccatcaa cgtgccactg atggcggcgc tggtcgggcc
     3121 gatgggggtg gagggcctgg cgctcgcgct gtcgatcagc gcggtgctgg aggtcatcgg
     3181 cctgctgtgg agcctgcacc ggcggatcga gtcggtggag gaggcgacca tcggacgttc
     3241 cgttgctcgt gccttcgttg ccggggtcgc cgcggccctg ctgatgctcg gcggcctcac
     3301 cgtggtggag gggtcactcg ccggatggct ggacaacccc gtcggtcggc tgctggcact
     3361 gctcgtgctc agctcggccg gcatcgccat cttcctcatc gtcgcggcgg cgctgcgcgc
     3421 tcccgagctg gaccagctgc gctcactgct gcggcgtcgc acccggaggc gcgcgtgatc
     3481 gacttccgac cggcgaccga tgcggacgcc accacctggc agtccctcct cgaggcgacg
     3541 gccacgggcg acttcttgca cgactgggcg tgggccgagg tcgcggcctt cgatggccag
     3601 ccgcagcgcc gatacgtgat cgagcaggcg ggcgaagtcg tgggcctggc ggcagcacag
     3661 gagcgccccc tcgtcgccgg ccgcagcttc tggtacgtgc cgcacgggcc cgtgctcgac
     3721 tgcgcgcatc cggacgcggc cgcccgtctg cgcgccatgc tgctgggcct gcgggccgcg
     3781 gcgcgccacc accgcgcggt ggcggtgaag ctcgagcccc ggctggctgc tgacgacccc
     3841 ggcgtcgcgc tcttcgcccg cctggagggc cgaagcctgc ggcggactga cgagaagctg
     3901 caggtcgggc agacgcggac ggtgcggctg gccgacgacg acgcgctgat tggcggcttc
     3961 gacaaggaca cgcgctacgg cgtgcgccgc gccgagcggg agggcgtggt cgtgcgcgtg
     4021 gtcgaggacg cgacggatgc gagcgccatc gacgccctcc acgagctcgt cggcgagacc
     4081 cagcgccggg ccggcttccc gatgccctcg cgggagcgct accgcatcgc ctggcgatcc
     4141 ctcgccggag cgggacgggc cgcgatcctc gaagcgagcc gcgagggcgt gctcctggcg
     4201 tcgggcatgg tggtcgtcga gggcgaccgc tccttctacc tgttcagtgg ctcgcggcgc
     4261 gaggaacccg gggagccgaa gcgctacgcg agctacgcgc tccagtggga gatgatgcgg
     4321 tacgcccgcg gtcggggcgt ccggctccac gatctgtggg gcatcgcgcc tgccggtgcc
     4381 ggatccgatc acccatggca cggggtgggc ctgttcaaga aggggttcgg cggcagcgag
     4441 gtggagtggg cgggatcctg ggacgtggtc gccgattcga cgctgtaccg ggtgcgcgcg
     4501 gcgctcggcg tcgcgcgcgg ctggctgcgc gggctgcggc ggctccggcc atgacgcagc
     4561 cgaccccgca ccagatgggc ctcggggcgc tgaccgatgc catcgcgccc gagcaggtga
     4621 tcggcatccc ggtcgggagt gtcacggcgc tcgcctacga ctcgcgccgc gtgaccccgg
     4681 gcacggtctt cttcgcggtc cccggcgtcc acgtcgacgg ccacgactac gccctcgatg
     4741 ccgcgcgggc cggcgccatc gcggcggtgg tcgagcgccc catcgatggc ctcgggatcc
     4801 cccagctggt cgtcgaccgc agccgccggg cgctggcgga cgccgccgac gcgtggttcg
     4861 gccatccatc aaaacgcctg gaggtcatcg gcatcaccgg aaccgacggc aagaccacca
     4921 ccgctttcct ggccgcggag gtgctgcgcg ccggtggacg gcggccgggg ctcatcggga
     4981 ccgtggccgt ggacgtcggc gacgagcggc gcagcaacga cgagcgcacc accacgcctg
     5041 aggcgctcga gctccaggca ctgctggccg acatggtggc ggccggcaac gacagcgtgg
     5101 tcatggaggc gacgtcgcac gggctggcgc tggagcggac gcggaactgc cgcttcgacg
     5161 tcggcgtggt caccacggtc accagcgagc acctggagtt ccacggcagc gtcgaggcct
     5221 accgtgccgc caaggcacgc ctggtcgagg aggcgccgct ggccatcctc aacgccgatg
     5281 atggcacctt cacctactac cgcgaccgag cccgcaaccg gctcctgacc tacgccatcg
     5341 acgccgatgc cgacctccgg gcgaccgacg ttcgggcgga cgcgtcgggc agccggtttc
     5401 gcctgcaggc gcccggctgg agcggcgacg tccacctgcc gatgccgggg cgcttcaacg
     5461 tcagcaacgc gctggcggcg ctggccgtgg cccacgccga gcaggtgccg ctcgacgcgg
     5521 cggcgggagc gctggagcgg acggccggcg ttccgggacg catggagcgc atcgatgccg
     5581 ggcagccgtt cgccgtcgtg gtcgactacg cgcacaccgc cgactcgttg gccaaggtgc
     5641 tgcgcatgct ccgtccggtg accacgggcc ggctggtggc ggtcttcggc tccgcgggcg
     5701 agcgggaccc gaccaagcga gagccgatgg gccgcgtcgc ggccgagctg gcggacctcg
     5761 tggtggtcac ggacgaggac cctcgcctgg aggacccgcg cgtcatcaac gagcagatcg
     5821 ccgacggcgc tcgcgccgcg ggtgcccgcg agggggagcg gctgtgggtc atcgacgatc
     5881 ggcgggaggc gatcggccgc gccatcggga tggccagacc cggcgatgtc gtcctgctgg
     5941 ccggcaaggg ccacgaggcc agcatcttct acggcaccga gaagcgaccc tgggacgacc
     6001 ggagcgccgc acgagaggcg ctggctgcgg caggatgggg gacagcatga cgagcaccga
     6061 tcttcgagcc ttccgcgtca cggacgcggc caggtggaac gccttcgtcg agtccgcgcc
     6121 gtaccacgcc ttcccgcagc tgtgggagtg gggcgaggtc cgggccatgg gcggctggcg
     6181 tccgatccgc ctggccgtcg gcccatccca ggacgagccc gtggccggcg tccagctcct
     6241 gctgcgaccg gtgccgctcg tcgactggca tctcgcctac gccccacgcg ggccgatcgg
     6301 ccgcctcgac gatcccgaga cgcgcgtcgc gctggtcaag gcgctgcggg tcctcggccg
     6361 cgaggagaag atcgccaccg ttcgcgcgga cccggaggcc acgcggcaga cgcactatgg
     6421 ccaggcgctg ctgtccgtgc cgtggcgggc cgcccccaag gtgcagccgc cgaccacccg
     6481 cgtcgtcgat ctgacggtgg gggaggagga gctgcggtcg aacctgaagc gcaagcaccg
     6541 ccagtacgtc aacaaggcag agcgggccgg cgtcaccgtc gagcggttcg atggc
//
LOCUS       DASTYP010000012         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1239514, whole genome shotgun sequence.
ACCESSION   DASTYP010000012 DASTYP010000000
VERSION     DASTYP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239514"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /gene="coxB"
                     /locus_tag="VFV59_00610"
     CDS             complement(<1..554)
                     /gene="coxB"
                     /locus_tag="VFV59_00610"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU5324367.1"
                     /translation="MLAAAGVFMLVVGLLAWSIVRYRGQPGRDVIVPDPSRGNVPLEI
                     AWWAAPTALVVVLVILTAGVLSQVDARAEEPDLTVQVQGFQWGWRFSFPDAGVEVTGT
                     PADPARIELPVGETIAFEITSRDVIHSFWIPPFLIKRDAVPGHPNRFDVTIEETGTYS
                     GQCGEFCGLLHSRQLFEITAVRPD"
     gene            complement(671..829)
                     /locus_tag="VFV59_00615"
     CDS             complement(671..829)
                     /locus_tag="VFV59_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324368.1"
                     /translation="MPPSLNRQGRYHALTLQIAEDGTTWQVTADQPFETRQEARSWAE
                     RLAAETPG"
     gene            961..1554
                     /locus_tag="VFV59_00620"
     CDS             961..1554
                     /locus_tag="VFV59_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324369.1"
                     /translation="MTTTPSTTMTGGTQGTTDEHPLAEATRDVGQSASEVVDRAANVG
                     FQRADQGKQQVAEGLGQLAGTIRRVSTDMESEQPMIANVAQTAADQTERIATFLRDTD
                     ARELLHTVEDVARRQPLLFLGGAFLLGAAASRFLKAGASGSAQPARGDRGSMRSGYRS
                     GYGSQYRTGYADATESYRATGPGASSGINGTLTDEGL"
     gene            1557..1952
                     /locus_tag="VFV59_00625"
     CDS             1557..1952
                     /locus_tag="VFV59_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5324370.1"
                     /translation="MAVREESRGIGDLLGDLGRQVSTLVRREIDLARVEVTSGLGRFG
                     RGAATTGIGGVLVYAGVLFVLAAIALGLIEAGLDAWLATLIVGAVVLVIGAVMASMGI
                     GQIRDARLAPTQTVETVREDVEYVKEQMK"
     gene            1955..2821
                     /locus_tag="VFV59_00630"
     CDS             1955..2821
                     /locus_tag="VFV59_00630"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEU5324371.1"
                     /translation="MERNDGVRLAATDAEQADLTVEDVEQADLDTDAETAETQEIRAD
                     IEETRVEMGGTLRELGDRLDPGNLMNEAKENVREATIGRVEETAKGMSEMVMETIRRN
                     PVPAAMAGAGLALLWMNRSNESRNGNGDAYRRYGAYDARYGDGYGYRGSGQGYSTESG
                     TGIADRAREAAAGVGENVSGAVGQVGETIGEVGQNVGETVGQGVEQASWRMERLMESS
                     PLAMGAIALGAGAVIGALIPETPHEERMLGEASRAVGETVRDTVAEATDRAESEMDRL
                     EREQSAPSPVGG"
     gene            complement(3121..>3462)
                     /locus_tag="VFV59_00635"
     CDS             complement(3121..>3462)
                     /locus_tag="VFV59_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324372.1"
                     /translation="DHTPLEVDVLDFSYRSMARLSRMKYSLCVLDARATDPLAGCNEL
                     ARRLSASSRSRQPILVLAASERDLRGPIRELAGRPNARIVSREAGPDGLRSAIDVLVG
                     AASATGPAMTA"
BASE COUNT          556 a         1204 c         1262 g          440 t
ORIGIN      
        1 tcgtccggcc ggacggcggt gatctcgaac agctgtcgcg agtgcaacag cccgcagaac
       61 tcgccgcact ggccgctgta ggtgcccgtc tcctcgatcg tcacgtcgaa gcggttcggg
      121 tgccccggca ccgcgtcacg cttgatgagg aacggcggga tccagaacga gtgaatgacg
      181 tcgcgcgagg tgatctcgaa ggcgatcgtc tcgccgaccg gcagctcgat gcgcgcgggg
      241 tcggccggcg tcccggtcac ctcgacgccg gcatcgggga acgagaagcg ccagccccac
      301 tggaacccct gcacctgcac cgtcaggtcc ggctcttccg cgcgcgcgtc cacctgcgac
      361 aggacacccg cggtgaggat caccagcacc accaccaggg ccgtcggtgc tgcccaccac
      421 gcgatctcca gcgggacgtt gccacgcgac gggtccggca cgatcacgtc gcgcccgggc
      481 tggccgcggt agcggacgat cgaccacgcc agcagcccga cgaccagcat gaagacgcca
      541 gcggccgcga gcatgaacca gttgtagagc tcgctgacct ggcggccgcg atcggtgacg
      601 ccttcgggga cgcaggcggc cgtggcgagg ccgatccccg ccagcgcgat gacggcgcgc
      661 gcgcgccggc tcagcccggt gtctccgcgg cgagccgctc ggcccaggag cgtgcctcct
      721 gccgggtctc gaaaggctgg tcggcggtca cctgccaggt cgtgccgtcc tccgcgatct
      781 gcagcgtgag ggcatggtat cggccctgtc gattgaggga gggcggcacg ccttggagat
      841 cctgcacggt cacgcgtgca gcagcgcgcg tgccacgccg gtcacgggca aaacctcgag
      901 cgcgtggaac gggcggtgca tcggagggct gcccacacca acttcgcaac gggggtcacc
      961 atgaccacga caccttccac caccatgacc ggcgggacgc agggcacgac cgacgagcat
     1021 ccgctcgccg aggccacgcg tgacgtcggc cagagcgcga gcgaggtcgt cgaccgcgcc
     1081 gccaacgtcg gcttccagcg cgccgaccag ggcaagcagc aggtcgccga agggctcggc
     1141 cagctggccg gcaccatccg ccgcgtttcc acggacatgg agtcggagca gccgatgatc
     1201 gcgaacgtcg cgcagacggc ggcggatcag acggagcgca tcgcgacctt cctgcgcgac
     1261 accgatgccc gtgagctgct tcacacggtc gaggacgttg cgcggcgcca gccgctgctc
     1321 ttcctcggcg gggcattcct cctcggtgca gccgcctcac gcttcctgaa ggcgggcgcc
     1381 tccggctcgg cccagcctgc gcgcggcgac cggggctcga tgcgcagcgg ctaccgctcc
     1441 ggctacggat cgcagtaccg caccgggtac gcagacgcca ccgagtcgta ccgggcaacg
     1501 ggcccgggcg ccagctcggg gatcaacggc acgctcacgg acgagggcct ctagtcatgg
     1561 ccgtccgtga ggagagccga ggcatcggcg atctcctcgg cgacctcggg cgacaggtga
     1621 gcaccctcgt ccggcgcgag atcgacctcg cgcgcgtcga ggtgacgagc ggcctgggac
     1681 ggttcggccg tggtgcggcg acgaccggca tcggcggtgt gctcgtgtac gccggggtcc
     1741 tgttcgtcct ggcggcgatc gccctcgggc tgatcgaggc cggcctggat gcctggctgg
     1801 cgacgttgat cgtcggcgcc gtcgtgcttg tcatcggcgc cgtcatggca tcgatgggca
     1861 tcggacagat ccgggacgcg cggctggcac ccacccagac ggtcgagacc gtccgcgagg
     1921 acgtcgagta cgtcaaggag cagatgaagt gacgatggaa cgcaacgacg gcgtccggct
     1981 ggcggcgacc gacgccgaac aggccgacct gacggtggag gacgtcgagc aggcggacct
     2041 cgacaccgat gccgagacgg ccgagacgca ggagatccgc gccgacatcg aggagaccag
     2101 ggtcgagatg ggcggcaccc tgcgcgagct gggtgaccgg ctggaccccg gcaacctcat
     2161 gaacgaggcg aaagagaacg tccgcgaggc gaccattggt cgcgtcgagg agacagcgaa
     2221 gggaatgagc gagatggtga tggagacgat tcgccgcaac ccggttccgg cggcgatggc
     2281 cggtgccggt ctggcgctgc tgtggatgaa ccgctcgaac gagagcagga acgggaacgg
     2341 cgacgcctac cgccggtacg gcgcctacga cgcccgctac ggcgatggct acggctaccg
     2401 tgggagcggc cagggctaca gcacggagtc cgggaccggc atcgcggacc gggcgcgcga
     2461 ggcagccgcc ggggtcggcg agaacgtctc gggcgcggtc gggcaggtcg gcgagacgat
     2521 cggcgaagtc ggtcagaacg tcggcgagac cgtcggccag ggcgtggagc aggcgtcgtg
     2581 gcggatggag cgcctcatgg agtcgagccc gctggcgatg ggggcgatcg ccctcggtgc
     2641 cggagccgtc atcggggccc tgatcccgga gacgccgcac gaggagcgca tgctcggcga
     2701 ggcgagccgc gcggtcggcg agacggtccg tgacaccgtc gccgaagcca ctgaccgcgc
     2761 cgagtcggag atggacaggc tcgagcggga gcagtcggcg ccgtcgccgg tcggcggctg
     2821 accgcacgca tgagcgaagg gcccgcaggc aaacgcctgc gggcccttcc tcgtgtccgg
     2881 tggtcctacg gaataccgag gcgagccgca ccgaagtgcg gcggccactc atccccgtcg
     2941 tcgtccggcc accacgcttc gtcctgggcc tcaacgcccg gccacggacc ttcgtccgtc
     3001 ggccgcccgg tcactcgcgg cgcggagcgt gcgggcagtg ccccgggcga agcgggcttc
     3061 agcccccgat gcaaaggctc cgattggaga accatctctc ctcccccttc tcggcgtctc
     3121 tcaggcggtc atggccgggc cggtcgcgct ggccgcgcca accagcacgt cgatcgcgct
     3181 gcgcaggccg tcaggccctg cctcgcgcga cacgatgcgc gcgttcggac gcccggccag
     3241 ctcgcgaatc ggcccgcgca gatcgcgctc cgacgccgcc agcaccagga tcggctgccg
     3301 gctgcggctc gacgcggaca gccggcgcgc cagctcgttg cagccggcta gcggatccgt
     3361 ggcacgggcg tccagcacgc acagcgagta cttcatgcgt gagagccgcg ccatggaccg
     3421 atacgagaaa tccaggacat ccacctcgag cggggtgtgg tc
//
LOCUS       DASTYP010000013         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_852, whole genome shotgun sequence.
ACCESSION   DASTYP010000013 DASTYP010000000
VERSION     DASTYP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFV59_00640"
     CDS             <1..823
                     /locus_tag="VFV59_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5324373.1"
                     /translation="VQRYATLEDLERHRTTIEEREEYEPHLEAGRVLYAGVDYEAILR
                     EADVIVWDGGNNDFPFYRPDLFVVVADPLRPGHEQRFHPGETNVRMAHVVVINKVDSV
                     DDATLRDELASIRSLNPNAQIVTARSTLRLEGGEVRGRRVVVVEDGPTLTHGGMTFGA
                     GIAAARRFGAADIVDPRPFAVGSIRDVLAKYPSLDRLVPAMGYGEEQMAELAETLNAT
                     DADLVLAGTPIDLTRVLRLDKPVVRVRYDLQEVGGPPLRDLLAPALAGATPRVPA"
     gene            947..1462
                     /locus_tag="VFV59_00645"
     CDS             947..1462
                     /locus_tag="VFV59_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU5324374.1"
                     /translation="MSVALPPTQPIRSEHAELLPQIEQLRHTAAWMADAPTPVVREKV
                     AHVVEFLEHHLVPHALAEDEVLYEAVEGAMGAPGASATMRRDHVEVERMTEVLAQLRD
                     DLTDPPSEQQRDRLTELLYGLHAVVALHFAKEEELYLPILDQALTVEDGERLMARMEE
                     AAERHRQFTHA"
     gene            1581..1763
                     /locus_tag="VFV59_00650"
     CDS             1581..1763
                     /locus_tag="VFV59_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324375.1"
                     /translation="MRSATIAAASVVLVGAYLAVAGTIGLAPAAIALGVSAIAVAAAV
                     VAVAASGRSDRHTFGA"
     gene            1841..2635
                     /locus_tag="VFV59_00655"
     CDS             1841..2635
                     /locus_tag="VFV59_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEU5324376.1"
                     /translation="MLRTVEITRYVTPLREGSSLPALAEADDGREYVIKFRGAGHGEK
                     ALIAEVIGGEIGRALGLPVPELVLAVLPGEIARGESHDEIRDLLGWSVGLNVGLAFVP
                     SALAADLTRPPAEGPDWAADVVWFDDLIVNPDRTPRNANLVVHEGRTWLIDHGSSLYF
                     HHAWTDPDAHARRPFELIGEHVLLPFASSIADADARLAPRLEPDTIGAIVDAVPDAWL
                     GDTRFDSPDGERAAYRRYLATRLEARPTWLEEADRRREEVQRDAVA"
     gene            2622..>2860
                     /locus_tag="VFV59_00660"
     CDS             2622..>2860
                     /locus_tag="VFV59_00660"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEU5324377.1"
                     /translation="MPSRDAFSYIVLRVVPDIEREEFVNAGVVLFCRPRRFLDAMIEL
                     DADALCALDPSCDLEGIRAQLEMVRAVAAGTLERAP"
BASE COUNT          393 a         1069 c          988 g          410 t
ORIGIN      
        1 cgtccagcgc tacgcgacgc tcgaggacct ggagcggcat cgcaccacca tcgaggagcg
       61 cgaggagtac gagccgcacc tggaggccgg tcgcgtcctg tacgccggcg tcgactacga
      121 ggcgatcctg cgcgaggccg acgtgatcgt ctgggacggc ggcaacaacg acttcccgtt
      181 ctaccggccc gacctgttcg tcgtggtcgc cgatccgctc cgcccggggc acgagcagcg
      241 cttccacccc ggcgagacga acgtccgcat ggcccatgtg gtggtcatca acaaggtcga
      301 cagcgtggac gacgcgaccc tccgcgacga gctcgcctcc atccgctcgc tcaacccgaa
      361 cgcccagatc gtcacggccc gctcgacgct gcggctggag ggtggcgagg tccgcgggcg
      421 ccgcgtcgtg gtcgtcgagg acggcccgac actgacccac ggcgggatga cgttcggcgc
      481 ggggatcgcc gcggcgcgcc gcttcggcgc ggccgacatc gtcgacccgc ggccgttcgc
      541 cgtcggttcc atccgtgacg tgctcgcgaa gtacccgtcg ctggatcggc tggtgcccgc
      601 catgggctat ggcgaggagc agatggccga gctggcggag acgctcaacg cgaccgacgc
      661 ggatctcgtc ctggccggca cgcccatcga cctcacccgg gtgctccgac tcgacaagcc
      721 ggtcgtgcgc gtccggtacg acctgcagga ggtcggcggc ccgccgctgc gcgacctgct
      781 ggcgcccgcg ctggccggcg ccacgccgcg cgtccccgcc tgagggtcag ccagaccgac
      841 gaccagcgac ccgttcgatc ggtcccttcg gcactgcgtg ccgcatgagc cgccggccac
      901 gctgatggcg acggctccgc ggcgagccca ccgagaggtg accgacatga gcgttgccct
      961 cccacccaca cagccgatcc gcagcgagca cgccgagctg ttgccgcaga tcgagcagct
     1021 gcggcacacc gccgcctgga tggcggatgc gcccaccccg gtcgtccgcg agaaggtcgc
     1081 gcacgtcgtc gagttcctgg agcaccacct ggtcccgcac gccctcgccg aggacgaggt
     1141 cctgtacgag gcggtggagg gcgccatggg cgcgcccggt gcatcggcga ccatgcggcg
     1201 agaccacgtc gaggtggagc gcatgaccga ggtgctggcc cagctgcgtg acgatctgac
     1261 cgatccgccc agcgagcagc agcgcgaccg gctgaccgag ctcctctacg gcctgcatgc
     1321 tgtggtggcc ctgcacttcg cgaaggagga ggagctctac cttccgatcc tcgaccaggc
     1381 actcaccgtc gaggatggcg agcggctgat ggcccgcatg gaggaagccg ccgaacggca
     1441 tcggcagttc acccacgcct gaccgcgatc cgcgtcgaat gtcgtaggtg acctcgcgcg
     1501 cctgaccgat gccgccgccc cggcggcggg tcgaccctga gcgacgtcgt tcatcactcg
     1561 tcgttcagga gtcgatccgc atgcgttccg ccaccatcgc cgctgcgtcc gtcgtgctcg
     1621 tcggtgccta cctggccgtc gccgggacca ttgggctcgc cccggcggcc atcgccctcg
     1681 gcgtcagcgc catcgccgtg gctgccgccg tcgtcgccgt cgccgcctcg ggccgctcgg
     1741 accggcacac cttcggagcc tgatctcgcg ccctcgcggt cactccatcg gctggcaccg
     1801 tgcgggcggc tggcccggcg cgcgcactac actcgcgccc atgctccgga ccgtcgagat
     1861 cacgcgctac gtcacgccgc tgcgcgaggg cagctcgttg ccggcgctgg ccgaggccga
     1921 tgacggccgc gagtacgtca tcaagttccg cggggcgggc cacggcgaga aggcgctgat
     1981 cgccgaggtc atcggcgggg agatcggtcg ggcgctcgga ctgccggtcc ccgagctggt
     2041 gctggccgtg ctgccggggg agatcgcccg cggcgagtcg cacgacgaga tccgcgatct
     2101 gctgggctgg tcggtcggac tgaacgtcgg tctcgccttc gtcccgagcg cgctggccgc
     2161 ggacctcacg cggcctccgg ccgaaggacc ggactgggcg gccgacgtcg tctggttcga
     2221 cgacctgatc gtcaacccgg accgcacgcc gcggaatgcg aacctcgtgg tccacgaagg
     2281 ccggacgtgg ctgatcgacc acggctcgtc gctctacttc caccacgcct ggaccgatcc
     2341 ggacgcccac gcccggcgcc cgttcgagct gatcggcgag cacgtgctcc tgcccttcgc
     2401 ctcctcgatc gccgatgcgg acgcgcgcct cgccccgcgc ctggagcccg acaccatcgg
     2461 cgccatcgtc gacgcggtgc cggatgcctg gctgggcgac acccgattcg actccccgga
     2521 cggcgagcgc gccgcctacc gccgatacct ggccaccagg ctcgaggcgc ggccgacctg
     2581 gctcgaggag gccgaccgac gacgcgagga ggtgcagcgc gatgccgtcg cgtgacgcct
     2641 tctcgtacat cgtgctgcgc gtcgtgccgg acatcgagcg tgaggagttc gtcaacgcgg
     2701 gcgtcgtcct cttctgtcgg ccgcgacggt tcctggacgc gatgatcgag ctcgatgccg
     2761 atgcgctgtg cgcgctggac ccctcgtgcg acctcgaggg catccgcgcc cagctcgaga
     2821 tggtccgcgc cgtcgcggcc ggcaccctgg agcgtgcacc
//
LOCUS       DASTYP010000014         4709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_219450, whole genome shotgun sequence.
ACCESSION   DASTYP010000014 DASTYP010000000
VERSION     DASTYP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219450"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFV59_00665"
     CDS             complement(<1..247)
                     /locus_tag="VFV59_00665"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU5324378.1"
                     /translation="MTEWIGGRRPVAEALAAGREAHRLLVSASARPSPELRHITDAAR
                     RMGLPTDRVPADALTRLAGFDGHQGVLLEVGERRFADL"
     gene            complement(244..1467)
                     /gene="ispD"
                     /locus_tag="VFV59_00670"
     CDS             complement(244..1467)
                     /gene="ispD"
                     /locus_tag="VFV59_00670"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU5324379.1"
                     /translation="MSAADRFSAIVVAAGRSSRMGSDKLWADLWGRPAWRWSVDTLLA
                     DAGLDHLAITVPPDAVERFASGLPPDAADRCLVVEGGEGRADSVIAGLWALTRAGHDD
                     ETLVLVHDAARPALAPDLVRSVVTTARATNAAVIPVVPVVDSLKRVRNDRVVGPIDRE
                     EVVSAQTPQAARLGVLRAAIEEAHAWGRPITDDAGALAAAGTPVHVVPGQSTNQKLTT
                     PADLVAVRAHLAGSITPIVPPFGVANLPAGTRCAVGFDAHRLVSGRPMRIGGLDFPDE
                     PRGPEGHSDGDAALHALIDALLGAAGAGDVGTLFPPGDAAWEGADSAELVRRAVARIG
                     ASGWVPASADVAVAVARPAIAPRRAEMAERIGALLGIGADAVSIKGTTSDGLGFAGSE
                     GIAAWAVAAVARHVP"
     gene            complement(1464..2525)
                     /locus_tag="VFV59_00675"
     CDS             complement(1464..2525)
                     /locus_tag="VFV59_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU5324380.1"
                     /translation="MTRIVQLLGALLGTLIGFALGLALLQRAGDLIQDANRPAFLTAF
                     VVATLLFGYLAIPYITIYPVRRAVEKLSEAGAADFTLGVAAIVVGLVMGLLIGVPLSQ
                     VGGPIGSWGPPIVALFLAVMMLAVTMEKRDVLLPAMAGLVPGAARRGAVTRVVIDTSA
                     VIDGRIADVAKTGFVLGTLVVPRFVLDELQRIADSPDAMRRNRGRRGLEMLSALQRDS
                     VTPIEISEATYPEVEEVDAKLMAYARDHGAAILTNDYNLNRVADLQGIRVLNVNELAN
                     AVKAVVHPGEELRVQIIQEGKEAGQGVGYLDDGTMIVVEGGVRFMDQEVPIAVTRVLQ
                     TVAGRMVFAQPRTDADGKE"
     gene            complement(2646..4022)
                     /gene="radA"
                     /locus_tag="VFV59_00680"
     CDS             complement(2646..4022)
                     /gene="radA"
                     /locus_tag="VFV59_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015373719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5324381.1"
                     /translation="MPVRSTIYVCQQCGASSPKWAGQCPSCAAWNTLVETAAAPRQPN
                     RAASASAARAVAGAVAVVPIGEVESAEAARLSSGIGEVDRVLGGGFVPGSIALFGGDP
                     GIGKSTLLLQLAARLARAGHSVLYVTGEESAGQVRGRAERVGAVVDNLHLAATTDLGT
                     ALGAIEAAGPALAVVDSVQTLSSEDLSGPAGSVGQVREVASRLAEVAKAGRTCVALVG
                     HVTKEGTVAGPRTLEHLVDAVIYLEGERLGATRLLRAAKNRFGSTDEVGVLEMRGDGL
                     AEVADASRFFLESEETPTAGAAITIALEGTRPLAVEIQALSAPSAYGSPRRATTGIDV
                     NRVHMLIAVLARHAGLNLSSHDVYVNVAGGLRIEEPAADLAVAMALASSLRDRPVQPA
                     TVLAGEIALSGRLRAATRGERRAIEATRLGFARLVTGPMRGGAAEGSRAITVARDVRE
                     AIGMALQD"
     gene            complement(4115..>4709)
                     /locus_tag="VFV59_00685"
     CDS             complement(4115..>4709)
                     /locus_tag="VFV59_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5324382.1"
                     /translation="LLQILEDGHLTDAKGRRVDFRNTVIIMTSNVGAQQLQRDTSIGF
                     RAGGSEAAQAAADYDRMKDKVLGELKNTFRPEFLNRIDATVVFRQLTREEIREIVDLL
                     LARVKNQLAGQEMELIVTDAAKDAIIVKGYDQAYGARPLRREIQNQIEDPLAERMLQA
                     SFSAGDTVIVDAGPDGGLTIQRQERETPKRQPAKAGR"
BASE COUNT          638 a         1748 c         1703 g          620 t
ORIGIN      
        1 ggaggtcggc gaagcgacgc tcccccacct cgagcagcac gccctggtgg ccgtcgaagc
       61 cggccagcct ggtcagggcg tcagccggca cgcggtcggt gggcaggccc atgcggcgcg
      121 ccgcatcggt gatgtggcgc aactccggcg acggacgggc gctggcggag accagcagcc
      181 ggtgcgcctc gcgcccggcg gcaagcgcct cggcgaccgg acggcggccg ccgatccact
      241 cggtcacggc acgtgccggg cgaccgcggc cacggcccag gcggcaatcc cctcgctgcc
      301 ggcgaagccc aggccgtcgg aagtggtgcc cttgatcgac acggcgtcgg caccgatgcc
      361 gagcagcgcg ccgatgcgct cggccatctc cgcccgacgc ggcgcgatgg cgggccgcgc
      421 gacggccacc gccacgtcgg cactggccgg gacccagccg gacgcgccga tgcgcgccac
      481 ggcccggcgg accagctctg cggagtccgc tccctcccac gccgcgtcgc ccggcgggaa
      541 gagggtcccg acgtcgccgg cgccggcagc gccgagcagg gcgtcgatga gcgcgtgcag
      601 cgccgcgtca ccgtccgagt gcccctcggg gccgcgcggc tcgtcgggga agtcgaggcc
      661 gccgatccgc atcgggcgac cgctgaccag ccggtgggcg tcgaagccga ccgcgcatcg
      721 cgtgccggcg ggaaggttcg cgacgccgaa cggcggcacg atgggcgtga tgctgccggc
      781 gaggtgggcg cggacggcga cgaggtcggc cggcgtggtc agcttctggt tggtggactg
      841 gccgggcacg acgtggacgg gcgtgccggc ggcggccagc gccccggcgt cgtcggtgat
      901 cggccggccc cacgcgtgcg cctcctcgat ggcagcccgc aggacgccga gccgcgcggc
      961 ctgcggggtc tgcgcggaca cgacctcctc gcggtcgatc ggtcccacca cccgatcgtt
     1021 gcgaacccgc ttcagcgagt cgaccaccgg cacgaccggg atgaccgccg cgttcgtcgc
     1081 gcgggcggtg gtgacgacgg agcggaccag gtccggcgcg agggccggcc gggcagcgtc
     1141 gtggacgagc acgagcgttt cgtcatcgtg gcccgcacgg gtcagggccc acaggccggc
     1201 gatcaccgag tcggcgcgac cctcgccgcc ctcaacgacc aggcaacggt ccgcggcatc
     1261 cggcggcagc cctgacgcga agcgctcgac ggcgtccggc gggacggtga tcgccaggtg
     1321 gtcgaggccg gcatcggcca ggagcgtgtc gacgctccag cgccacgccg ggcgtcccca
     1381 gaggtccgcc cacagcttgt cgctccccat ccggctgctg cggccggccg cgacgacgat
     1441 ggcgctgaag cgatcagcgg cgctcattcc ttcccatccg catcggtgcg cggctgtgcg
     1501 aacaccatgc ggccggccac ggtctggagg acgcgggtga cggcgatggg cacctcctgg
     1561 tccatgaagc gcacgccgcc ctcgaccacg atcatcgtgc cgtcgtcgag atacccgacg
     1621 ccctgccccg cctccttgcc ctcctggatg atctggaccc gcagctcctc gccggggtgc
     1681 accacggcct tcaccgcgtt cgccagctcg ttgacgttga ggacgcggat gccctgcagg
     1741 tcggcgacac ggttgaggtt gtagtcgttg gtcaggatgg ccgcaccgtg gtcacgcgcg
     1801 taggccatca gcttggcgtc gacctcctcg acctccgggt aggtcgcctc gctgatctcg
     1861 atcggggtca ccgagtcccg ctgcagcgcg gacagcatct cgagcccgcg ccggccccgg
     1921 ttgcggcgca tggcatcggg cgaatcggcg atgcgctgga gctcgtccag gacgaagcgc
     1981 ggcaccacca gggtcccgag cacgaacccg gtcttcgcga catcggcgat gcgcccgtcg
     2041 atgacggcgc tggtgtcgat caccacccgc gtcaccgcgc cgcggcgagc cgccccgggg
     2101 accaggccgg ccatggccgg cagcaggacg tcgcgcttct ccatggtgac ggccagcatc
     2161 atgaccgcca ggaagagggc gacgatgggc ggtccccagg agccgatcgg tccgcccacc
     2221 tgcgagaggg ggacgccgat gaggagcccc atgacgagcc cgacgacgat cgccgccacg
     2281 cccagcgtga agtcggcggc cccggcctcg ctcagcttct cgacggcgcg ccgcaccggg
     2341 tagatggtga tgtacgggat ggcgaggtag ccgaacagca gcgtggcgac gacgaaggcc
     2401 gtcaggaacg ccggtcggtt cgcgtcctgg atgaggtcac cggcacgctg caggagcgcc
     2461 aaccccagcg cgaagccgat gagggtgccg agcagggcgc ccagcaactg gacgatgcga
     2521 gtcatgtgtt cgatgagtcc gtgatgacgg cgtcgaccgc gggatgattc gatgaatgcg
     2581 gcgacggacc ggtcggccac gttagcacgc gcctccgggg gcgtccacgt tccctcggtc
     2641 gcggctcagt cctgcagcgc catcccgatc gcctcgcgga cgtcgcgcgc cacggtgatc
     2701 gcgcgcgacc cttcggccgc gccaccgcgc atcggcccgg tcaccaggcg cgcgaagccc
     2761 aggcgcgtgg cctcgatcgc ccggcgctcc ccgcgcgtgg cggcgcgcaa ccggccggac
     2821 agggcgatct cgccggcgag gacggtcgcc ggctgcacgg ggcgatcccg gaggctggat
     2881 gccagggcca tggcgaccgc cagatcggcc gcgggttcct cgatgcggag tccgccggcc
     2941 acgttgacgt agacgtcgtg actcgacagg ttcagcccgg catgccgcgc caggacggcg
     3001 atcagcatgt gcacgcggtt gacgtcgatg ccggtcgtcg cccgtcgcgg cgagccgtag
     3061 gccgacggcg ccgacagcgc ctggatctcg acggcgaggg gcctggtccc ctccagcgcg
     3121 atcgtgatgg cggcgccggc cgtcggcgtc tcctccgact cgaggaagaa gcggctcgcg
     3181 tcggcgacct ccgccagccc gtcgccgcgc atctccagca cgccgacctc gtcggtcgac
     3241 ccgaaccggt tcttggcggc gcgcagcagc cgggtggcgc ccagccgctc cccctccagg
     3301 tagatcacgg cgtccaccag gtgctccagc gtcctgggcc ccgcgaccgt gccctccttg
     3361 gtcacatgcc cgaccagcgc cacgcaggtg cgacccgcct tggccacctc cgccaggcgc
     3421 gaggcgacct cccgaacctg gccgacgctc cccgccggcc cgctgaggtc ctcgctgctg
     3481 agcgtctgga cgctgtcgac gacggccagg gccggtccgg ccgcctcgat ggcgcccagc
     3541 gccgtgccca ggtcggtcgt ggcggcgagg tgcaggttgt cgacgacggc gccgacccgc
     3601 tccgcccgac cgcggacctg gcccgcggac tcttcgcccg tcacgtacag caccgaatga
     3661 ccagcacggg ccaggcgcgc cgcgagctgg agcagcagcg tcgacttccc gatgccagga
     3721 tcaccgccga acagggcgat cgagccaggg acgaagccac caccgagcac acggtccacc
     3781 tcgccgatgc ccgaggacaa ccgtgccgcc tcggcgctct cgacctcgcc gatgggcacg
     3841 accgccacgg cgcccgcgac cgcccgcgcc gccgaggcgg aggcggcgcg gttgggctgg
     3901 cgaggtgccg ctgccgtctc caccagcgtg ttccaggccg cgcacgacgg gcactgcccc
     3961 gcccacttgg gcgaggaggc gccgcactgc tggcagacgt agatggtgga tcggacgggc
     4021 acgcggactc ctcgggcgac gtcgggccat cttgccacgg ccgaacggcc gcggcgctta
     4081 ccgccggctt accgcgcggc ggtgccgggg accgtcagcg accggccttc gccggctggc
     4141 gcttgggcgt ctcccgctcc tgtcgctgga tcgtcaggcc gccatccggc ccggcgtcca
     4201 cgatgacggt gtcgccggcg ctgaagctcg cctggagcat gcgctccgcc agcgggtcct
     4261 cgatctggtt ctggatctcg cgacgcagcg gccgcgctcc gtacgcctgg tcgtagccct
     4321 tgacgatgat cgcgtccttg gcagcatcgg tgacgatcag ctccatctcc tggccggcca
     4381 gctggttctt cacgcgagcc agcagcaggt ccacgatctc gcggatctcc tcccgggtca
     4441 gctggcggaa gacgacggtg gcgtcgatgc ggttcaggaa ctcggggcgg aacgtgttct
     4501 tcagctcgcc gagcaccttg tccttcatcc ggtcgtagtc cgcggccgcc tgggcagcct
     4561 cgctgccgcc ggcccggaag ccgatgctcg tgtcgcgctg cagctgctgc gcgccgacgt
     4621 tgctggtcat gatgatgacc gtgttgcgga agtcgacgcg gcggcccttc gcatcggtca
     4681 ggtggccgtc ctcgaggatc tgcagcagg
//
LOCUS       DASTYP010000015        13827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1239575, whole genome shotgun sequence.
ACCESSION   DASTYP010000015 DASTYP010000000
VERSION     DASTYP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13827
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239575"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFV59_00690"
     CDS             complement(<1..674)
                     /locus_tag="VFV59_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324383.1"
                     /translation="MSHHRRQSLAIAFLVLLLVVPWAPRSARAAEPSLGEAVTLADGT
                     VLPPMPAELVAPSVHEEMLIEHGGDAEGAVGGIQSAPETASATDGAVQADGLVVAQAG
                     ATGPAGALPNGLRREVFGFLPYWKLDATTRSALRLDLVSTIAYFGIGVQSDGRLIRGT
                     STGWTGWASSAMTELMNAAHARGVRVVPTITLMSWDGDYTNLSTLLNDPAARQTLVAD
                     VATIIR"
     gene            complement(790..2661)
                     /locus_tag="VFV59_00695"
     CDS             complement(790..2661)
                     /locus_tag="VFV59_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEU5324384.1"
                     /translation="MRDRIRAAFLAAVTLAMSVMVAAPTAVSAAGPKVAVIVGPVGSI
                     TDQYRTMADRVASAATTAGASVVKVYSPNATWANVKAAVNGANVIVYFGHGNGYPNPY
                     TSGTEYTDRVNGFGLNTATTNGDADSWSAGTLVYCGEKALLGTLSSTDGAAQRQYCGG
                     VNGGPITPAAGFTMVYAQAHYAPGFGERYTPETPLTTLDEAKQRVTNYSTPILRLGGT
                     YFATAYGDADQIVSRVLAAGSTTYGQIFSAGRGYSASTLTEVPHPDVSGAVVRVQQTV
                     ISSLHFGDPDYWYAFAGNPNASPGLAAGTPVAPPAPSAPPAQLDVTRLAGGDRYETAA
                     AISAASFAPGVAVAYVAVGSNFPDALAAGAAAARRGGPVLLVTPGGVPASTAAELARL
                     RPSAIKVVGGAGVIGDGVLAALRPYATSGSVSRVAGDNRFATAAAISADTFSPGVPVA
                     YVATGRNFPDALAGVAAAGSRGGPILLTAPSELSPETAAELARLRPATIVVLGGAGVV
                     GDGVLAALRGYATTGSVARLAGQDRYATAVAVSTGTFSASQTVFVATGANFPDALGGG
                     PVAATVPGPLLLVPGSTLPSSVAAELQRLGPDRVVILGGVGVVSDGVIGQIGSLLGG"
     gene            complement(2790..3974)
                     /locus_tag="VFV59_00700"
     CDS             complement(2790..3974)
                     /locus_tag="VFV59_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5324385.1"
                     /translation="MTDTPRRVAIVVHAIFPGDPRVHRQADALVDAGFEVDVLCLRQP
                     GEPAEERDGAVRIVRLPVNRSFVGFAGHIAEYLAFTAIATWRLAREHRSRRYALVQVA
                     TVPDFLAFAAVPEKLGGAPLLLDLHEDMPEFFRDRFASPALRPLLPVVTAATRASAAV
                     ADELITVHEPLRRLSIERGVPPEKISVVMNSADGRLFDPSRHERRAFMADGTLRLIHH
                     SNLQRIYGLDRAIEGLATLRPDLDWRLDVYGDGPWRPQVEAAIERSATADRVTLHGRV
                     PMSDLPGLLAGADIGLVPSLDEPYLRYSLSTKLLEYAAMGVPAIASDLATFRHHFTDA
                     ALRYVPGGDGSALARAVEELVDDPERTVAMGLEAQRQAAAYDWEVQKRRYVAIVRRLI
                     RE"
     gene            complement(3971..5074)
                     /gene="mazG"
                     /locus_tag="VFV59_00705"
     CDS             complement(3971..5074)
                     /gene="mazG"
                     /locus_tag="VFV59_00705"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU5324386.1"
                     /translation="MRAGSRRSLDALLEAARLTPEDGVQIVGARRLPAVAFDPSLPLL
                     VLRGGSEADPLPGRHARRGAVEVLRTLYPDAHQVRRLPDDGTLSLAELTDEAMADDDW
                     FVPPLAPADNLASPHGMAAISARLRAPDGCPWDRKQTHLTLRPYLLEEAYELVDAIEN
                     GTPDDLAEELGDLYLQVVLHAQFAAEEEQFDLTDVYRSIAAKIVRRHPHVFGEVSVDG
                     VEQVISNWEQIKAGEREERGKGHEGAFAGIARALPAVPASREIQERASTLGWDWDAID
                     GVWEKVTEELDELREAATDDERRHELGDVLFALVNLGRWMKLDPEEALRAANHRWVAR
                     YHRVEALAAERGVDLDALSLAEKDELWDEVKGT"
     gene            complement(5079..6815)
                     /locus_tag="VFV59_00710"
     CDS             complement(5079..6815)
                     /locus_tag="VFV59_00710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5324387.1"
                     /translation="MTLRSRPVLDRKHRPRWQDELRTQQLTIIGFAVAIAVALGIFGA
                     AAWNGYWESHFRPVAAVGDTTFDRADLEQRTKILTAEAIARVTELQAQLTGSARDQVI
                     QQQIDQISVAFNSLDTDAAQSLVDQQVLAARADELGISVSDDDVDAALAERYALPERV
                     RARLILVDSLPDDAQPDDEPTDEQKAAAEEAAQAARARVEGGEDFATVATEVSDDFTS
                     SIGGELGWFQADDSAYGEYFDSLADAEVGDLVGPVETERGYAVLELLDRREATSDGGL
                     RQVLADQGVDESTYRDYVRAAALEQAYRDYFSETVVTSPAAQQRVAQIRIAPVSGTVV
                     PQERARHILVQPLPDADDQTQATEAQWEEALAEARDIEAQLKEDGADWWAIAEESSDD
                     PGSASRGGDLGWYDPANPPFVEPFSVELSNLEVGDISDPIRTDFGWHVIQKTAERESP
                     TAEAADLVEQLRADRDSFADVAKDVSEDYETANDGGELGWVAHYQLGAAQEDAIFGLT
                     DVGEISDSVDTGSNGIFIYQLLESSDSREIETDRLDEIRTSGFQRWLDEEVRAGADVW
                     VDPQYATSTATG"
     gene            complement(6919..7710)
                     /locus_tag="VFV59_00715"
     CDS             complement(6919..7710)
                     /locus_tag="VFV59_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEU5324388.1"
                     /translation="MSASAAEAADLPSVGLAAGRGVWVVLPTYNEHDNLEPMLDAILA
                     ALPEASVLVVDDHSPDGTGELADTIAAGEPRVRVLHRSGKEGLGVAYRDGFRWVLEHP
                     ETRAVVQMDADFSHDPADLPRLLAPLMRDADLALGTRYMRGGGTVGWPWYRKLISRGG
                     TIFARTVLLLPYRDLTGGFKAWRRELIDAIRLRETSGSGYGFQIETTWWAHRRGARIV
                     QVPIVFRERVVGTSKMTGGIVREAMLLVLRLRWRSIADALRGGRD"
     gene            complement(7691..8968)
                     /locus_tag="VFV59_00720"
     CDS             complement(7691..8968)
                     /locus_tag="VFV59_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU5324389.1"
                     /translation="MKIAVVGLGHIGLPLAVQYASRGHDVLGVDVADPIVDSINRGES
                     PHRDEQTLIDRVPELHAAGRLRATTWRDAEGVREADVVVVIVPVVVDADRQIDFGPID
                     AATADIARHVKPGALVVYETTLPVGTTRNRFGPMLARGSGLELDRDLFLAFSPERVLV
                     GRVFLDLQRYPKIVGGTSEESTRRAVEFYRAVLDEGTDVWAVTNAETAEMTKLAETTY
                     RDVNIAYANELARFAQRNGIDVSEVIGAANSQPYSHIHQPGVGVGGHCIPVYPHFLFN
                     DDPELQIPPLARQINEGMGEFVVDVIEDRIGSLDEVPVVVLGVAYRGDVKEDAFSSAF
                     RLRDELLAAGAQVYAHDPFFDDDHLRALGFEPYDLNSAQPVKVAILQAAHSAYQDLDP
                     SKLPGLELFVDGRNVLDRERFDRAGVGYVGVGR"
     gene            complement(8965..10017)
                     /locus_tag="VFV59_00725"
     CDS             complement(8965..10017)
                     /locus_tag="VFV59_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5324390.1"
                     /translation="MSEQRPLRAGVIGLGMMGRNHVRVWDEAVAGVELVAVADPDTAA
                     VERATAGRRARGYAEIERMLAEEELDLVSIVAPTSLHLPVALAALRAGVNVLVEKPIA
                     ATRAEAVEMIEAAASAGRLLTVGHIERFNPAVRELKRRLDAGELGRIFQISAQRLGPF
                     PARIRDVGVVVDLGPHDLDIIRYLVGDDPIRIYAETERRIHTEHEDLFNGIMKFGNGV
                     VGVVDINWLTPTKKRQLTVTGERGMYVADYISQDLVFYANPDAPDTWINRGAAGLGAQ
                     VTSVSEGEMVRRSVRRQEPLAVELGEFARAVRDGGPPPVDPHDAMVALLLARKMVESA
                     LSGEVIAGPALQEVLS"
     gene            complement(10014..11120)
                     /locus_tag="VFV59_00730"
     CDS             complement(10014..11120)
                     /locus_tag="VFV59_00730"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5324391.1"
                     /translation="MIPIARPQMGEEEKERVWDAMASGSLAQGPRVHQFEDEFAAMVG
                     AGHAVATSSGTTALHLALLGYGVGEGDEVITVPFTFIASANSILYTGARPVFVDIDER
                     DFTMDPAQVEAAITPRTKAIMPVSLYGQPADLPAIAEIAERHGLALVEDAAQAHGAAV
                     DGRRSGTWGAGTFSFYPTKNMTTGEGGMVTTDDAELAERIRLLREHGMKVRYHHDVVG
                     YNFRMTDIAAAIGLAQLPKLAGYNDRRRAIAARYDAELHGVITPWVRPGVTHVYHQYT
                     IRVNQRDAFAERLKERGVGSAIYYPIPVHRQKPFLALGYGDQSYPVTDRLTEQVLSIP
                     VHPALTDDEVSTVVEAVNATAEEVGPIPAEAARA"
     gene            complement(11117..12235)
                     /gene="wecB"
                     /locus_tag="VFV59_00735"
     CDS             complement(11117..12235)
                     /gene="wecB"
                     /locus_tag="VFV59_00735"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU5324392.1"
                     /translation="MKVLTVVGARPQFVKAAPVSRALREAHTEILVHTGQHYDEAMSA
                     AFFRDLEIPEPDVNLEVGSGSHGAMTGEMMRRLEPVVLEHEPDGVLVYGDTNSTLAAA
                     VVTCKLAYPDGRRPWLAHVEAGLRSFNPRMPEERNRIVADHLAELLLAPTEAAMEHLA
                     REGLADRAELAGDVMVDAHAWAAARADEHLPAVAREHDRYVLLTMHRAENVDHPDRFR
                     RILAGLELDLPVIFPVHPRTRAILERGTHGLPSNVVPVEPAGYLEMVALEARAHAIAT
                     DSGGVQKEAYLSGVPCVTLRSETEWVETVAAGWNRVVDDDPAALRAALGDDDFMSRDK
                     PRPPLYGDGRAAARIVAALERHDARAVTAPMPEEAPAR"
     gene            complement(12232..12840)
                     /locus_tag="VFV59_00740"
     CDS             complement(12232..12840)
                     /locus_tag="VFV59_00740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5324393.1"
                     /translation="MAERAQVRIHPTADVSPEAEIGPGTSIWNQAQVRERARIGSRCI
                     IGKNAYVDFDVVLGDDVKVQNNVSIYHGVTVEDGVFIGPHVCFTNDLIPRAINPDGSL
                     KTDADWEVAPIRVRHGAALGANSTILPGVTVGRWAMVGSGAVVTRDVADYELVVGNPA
                     RRLGSACPCGQPLRDTEDGEPFRGTCPRCGEAFPPSASGAAG"
     gene            complement(12877..13731)
                     /gene="mreC"
                     /locus_tag="VFV59_00745"
     CDS             complement(12877..13731)
                     /gene="mreC"
                     /locus_tag="VFV59_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5324394.1"
                     /translation="MYGPASPHRRSRTGLWFAAFTIASLLMLLASSTEPARTLQHVTT
                     RALDPVRSAIGGVGAGVAGLFGTIGEIDRLRGENAELRRDLNGAEQRIAELTEAAREN
                     AELRDLLGIRDALQMTTLPVRVIRRDPSNFAWEVGVDAGSRDGIRVGMPVVGNADAVG
                     ALAGTIVAVTDDTATVRFVVDTRSRVIALDQASRALGEVRGQPGGQLVMTQIPVTELV
                     AVGDAIVTAGLTTDEEASRFPGGLLIGTVQAVETDANALTQTAFVRPAFDVHSAERLL
                     VVMEFDQG"
BASE COUNT         1869 a         4888 c         5080 g         1990 t
ORIGIN      
        1 tcgcggatga tggtcgccac gtccgcgacc agcgtctggc gcgctgccgg gtcgttcagc
       61 agcgtcgaca ggttggtgta gtcgccgtcc cagctcatga gcgtgatggt cggcacgacc
      121 cgcacgcccc gcgcgtgggc ggcgttcatc agctcggtca tggccgagga cgcccagccc
      181 gtccagccgg ttgacgtgcc gcggatgagg cggccgtcgc tctgaacgcc gatgccgaag
      241 tacgcgatgg tcgataccag gtccagccgc agggccgagc gcgtggtcgc gtcgagcttc
      301 cagtacggga ggaagccgaa cacctcgcgc cgcagcccgt tggggagcgc gcccgccggg
      361 ccggtcgcac cggcctgggc gacgacgagt ccatcggcct ggacggcacc gtcggtggcc
      421 gatgcggttt ccggcgcgga ctggatcccg ccgaccgcgc cttcggcgtc gccgccgtgc
      481 tcgatgagca tctcctcgtg gaccgatggg gccacgagct cggccggcat gggcgggagg
      541 acggttccgt cggcaagcgt gacggcctcg ccgagcgacg gctcggcggc acgtgcgctc
      601 cgcggcgccc acgggacgac gaggagcagg acaaggaagg cgatggcgag ggactggcgg
      661 cggtggtgcg acatgatcac tcgtgtggcg ggctggtggc tcgccttcgg gcgaaccgca
      721 ccgcacccta ccgttccctc accctctccc caatggtacc tctgggggat gctcgtccct
      781 cgcccccgat catccgccga ggagggaccc gatctggccg atgacgccgt ccgacacgac
      841 gccgacaccg cccaggatga cgacgcgatc ggggccgaga cgttgcagct ccgccgcgac
      901 cgaggacggc agcgtgctgc cgggcaccag caggagcgga cccggcaccg tggccgcgac
      961 cggaccgccg ccgagcgcgt cggggaagtt cgcgccggtg gccacgaaga cggtctggct
     1021 cgcgctgaag gtgccggtgg agacggcgac ggccgtcgcg tagcgatcct gtccggccag
     1081 gcgcgcgacc gagcccgtcg tcgcgtagcc gcggagggcg gcgaggacgc cgtcaccgac
     1141 cacgccggcg ccacccagca ccacgatggt cgccggccgc agccgcgcga gctccgcggc
     1201 cgtctcgggg ctcagctcgc tcggcgcggt gagcaggatc ggcccgccgc gcgagccggc
     1261 ggcggcaacg ccggccagcg catcggggaa gttgcggccg gtggccacgt acgccaccgg
     1321 gacgcccggc gagaacgtgt cggcgctgat cgcggctgcc gtcgcgaacc ggttgtcgcc
     1381 ggccacgcgg ctcacgctgc ccgacgtcgc gtaggggcgc agggcggcga gcacgccgtc
     1441 accgatcacc ccggcgccgc cgacgacctt gatggccgac ggccgcaggc gcgcgagctc
     1501 cgcggccgtc gatgcgggga cgccacccgg cgtcacgagg aggaccgggc cgccacgccg
     1561 ggcggcggcc gcaccggcgg ccagcgcatc cgggaagttg ctgccgacgg ccacgtaggc
     1621 caccgcaacg ccgggcgcga agctggcggc actgatggcg gcggcggtct cgtatcgatc
     1681 acccccggcc agtcgcgtca cgtccaattg ggccggcgga gcgctcggcg cgggcggggc
     1741 gaccggcgtg ccggcggcca ggccggggct cgcgttcgga ttgcccgcga acgcgtacca
     1801 gtagtcgggg tccccgaagt ggaggctgct gatgaccgtc tgctgcacgc ggaccacggc
     1861 gcccgacacg tcggggtgcg ggacctcggt gagcgtcgag gcgctgtagc cccggccggc
     1921 ggagaagatc tggccgtagg tcgtcgagcc cgcggcgaga acacgggaga cgatctggtc
     1981 ggcatcgccg taggcggtcg cgaagtaggt tccgccgagg cgcaggatcg gcgtcgagta
     2041 gttggtgacg cgctgcttgg cctcgtcgag cgtggtcagc ggcgtctccg gggtgtagcg
     2101 ctcgccgaag ccgggggcgt agtgcgcctg tgcgtagacc atggtgaagc ccgcggcggg
     2161 agtgatgggg ccgccgttca cgccgccgca gtactggcgc tgtgcggcgc catcggtgct
     2221 gctcagggtg ccgaggaggg ccttctcgcc gcagtagacg agcgtgccgg cggaccagct
     2281 gtccgcgtca ccattcgtcg tcgccgtgtt gaggccgaag ccattgacgc ggtcggtgta
     2341 ctccgtcccg ctggtgtagg ggttcgggta gccgttgccg tggccgaagt agacgatgac
     2401 gttcgcgccg ttgacggctg ccttgacgtt cgcccacgtg gcgttgggtg agtacacctt
     2461 gacgaccgac gcgcccgcgg tggtggccgc gctcgccacg cggtcagcca tggtgcggta
     2521 ctggtcggtg atggacccca cgggcccgac gatgacggcc accttcgggc cggcggcgct
     2581 gacggcggtt ggggcggcga ccatgacgga catggccagg gtgacggcag ccaggaaggc
     2641 ggcgcgaatt cggtctcgca acaggggatc ctcgaggtgg tcgcagggct ggtcgggaag
     2701 gcacgtctcc ccagccgcga tcgtaggaac cgatgcgccc gggccctatg ggtcgatcgt
     2761 ccgatcctcg tctggcccca tcggacccgt cactctcgga tgagccgccg gacgatggcc
     2821 acgtaccggc gcttctggac ctcccagtcg taggcggccg cctggcgctg ggcctcgagg
     2881 cccatggcca ccgtccgctc cgggtcgtcg acgagctcct cgaccgcccg ggcaagggcg
     2941 cttccatcgc cgccggggac gtaccgcagc gccgcatcgg tgaagtggtg gcggaacgtc
     3001 gccaggtcgc tggcgatggc cggcaccccc atggcggcgt actcgagcag cttggtgctg
     3061 agcgagtagc gcaggtacgg ctcgtccagc gagggcacca gaccgatgtc ggcgccggcg
     3121 agcagccccg gcagatcgct catgggaacg cgaccatgga gcgtgacccg gtcggcggtc
     3181 gcgctgcgtt cgatcgcggc ctccacctgc ggccgccacg ggccgtcgcc gtacacgtcc
     3241 aggcgccagt cgaggtccgg ccgcagcgtc gccagcccct cgatggcgcg gtcgaggccg
     3301 tagatgcgct gcaggttgga gtggtggatg aggcgcagcg tgccatcggc catgaaggcg
     3361 cggcgctcgt ggcgggacgg gtcgaagagg cggccgtcgg cgctgttcat gaccacgctg
     3421 atcttctcgg gcgggacgcc tcgctcgatc gagagacgcc gcagcggctc gtggacggtg
     3481 atcagctcgt cggcgacggc cgccgaggcc ctggtggccg cggtcacgac cggcagcagc
     3541 ggccgcagcg cgggtgacgc gaatcggtca cggaagaact ccggcatgtc ctcgtgcagg
     3601 tcgagcagca gcggggcgcc gcccagcttc tccggcaccg ccgcgaaggc caggaagtcg
     3661 ggcaccgtcg cgacctgcac cagggcgtag cgtcgtgacc gatgctcccg cgccagccgc
     3721 caggtggcga tggcggtgaa ggccaggtac tcggcgatgt gcccggcgaa gccgacgaag
     3781 ctgcggttga ccgggagccg cacgatgcgg accgcgccat cgcgctcctc ggccggctcc
     3841 ccgggctggc gcaggcacag cacgtcgacc tcgaagccgg cgtccaccag ggcgtcggcc
     3901 tggcggtgga cccgcgggtc gccgggaaag atggcgtgga cgacgatggc gacgcggcgc
     3961 ggcgtgtccg tcacgtgccc ttcacctcgt cccacagctc gtccttctcg gccagcgaca
     4021 gcgcgtccag gtccaccccg cgctcggcgg ccagcgcctc gacgcggtgg tagcgggcca
     4081 cccagcggtg gttggcggca cgcagcgcct cctcggggtc aagcttcatc cagcgaccca
     4141 gattcacgag ggcgaagagc acgtccccga gctcgtggcg gcgttcgtcg tcggtggccg
     4201 cctcgcgcag ctcgtcgagc tcctccgtga ccttctccca cacgccgtcg atggcatccc
     4261 agtcccagcc gagcgtcgac gcccgctcct ggatctcgcg gctggccggt acggctggaa
     4321 gggcgcgggc gatgccggcg aacgccccct cgtgcccctt cccgcgttcc tcgcgctccc
     4381 ccgccttgat ctgctcccag ttgctgatca cctgctcgac accgtcgacg ctcacctcgc
     4441 cgaagacatg cgggtggcga cgcacgatct tggcggcaat ggaccgatac acgtcggtca
     4501 ggtcgaactg ctcctcctcg gccgcgaact gggcgtgcag caccacctgc aggtagaggt
     4561 cgccgagctc ctcggccaga tcgtcgggcg tcccgttctc gatggcatcg accagctcgt
     4621 aggcctcctc gagcaggtac ggccgcagag tcaggtgcgt ctgcttccga tcccacggac
     4681 agccgtcggg cgcgcgcagc cgcgcggaga tggcggccat gccgtgcggg ctggccaggt
     4741 tgtccgccgg tgcgaggggc ggcacgaacc agtcgtcgtc ggccatggcc tcgtccgtca
     4801 gctccgcgag ggacagcgtg ccgtcgtccg ggaggcggcg aacctggtgc gcatcgggat
     4861 acaacgtccg cagcacctcg accgccccgc gacgagcgtg gcgtccggga agcggatcgg
     4921 cctccgaccc gccgcgaagg accagcagcg gcagcgaggg atcgaaggcg acggcgggaa
     4981 ggcgtcgtgc gccgacgatc tgcaccccgt cctcgggcgt gagccgcgcg gcctccagca
     5041 gcgcgtcgag gctgcgtcgg gagccggcgc gcatcggctc agccggtggc cgtcgaggtg
     5101 gcgtactgcg ggtcgaccca gacgtccgcg ccggcacgaa cctcctcgtc cagccagcgc
     5161 tggaagccgg aggtgcggat ctcgtcgagc cggtcggtct cgatctcgcg gctgtcgctc
     5221 gactcgagca gctggtagat gaagatgccg ttgctgcccg tgtcgaccga gtcgctgatc
     5281 tcgccgacgt cggtcaggcc gaagatcgcg tcctcctgcg ccgcgccgag ctggtagtgc
     5341 gccacccagc ccagctcacc gccgtcgttc gccgtctcgt agtcctcgct cacgtccttc
     5401 gcgacgtcgg cgaaggagtc gcgatcggcg cgcagctgct cgaccaggtc ggcggcctcg
     5461 gccgtcgggc tctcacgctc ggccgtcttc tggatgacgt gccacccgaa gtcggtgcgg
     5521 atcgggtcgc tgatgtcgcc cacttcgagg ttggacagct ccacgctgaa cggctcgacg
     5581 aagggcgggt tggccgggtc gtaccagccg aggtcgccgc cgcggctggc cgatcccggg
     5641 tcgtcgctcg actcctcggc gatcgcccac cagtcggcac cgtcctcctt gagctgtgcc
     5701 tcgatgtcgc gcgcctccgc cagcgcctcc tcccactgcg cctcggtcgc ctgggtctgg
     5761 tcgtcggcgt cgggcagtgg ctggaccagg atgtggcgcg cccgctcctg cggcacgacg
     5821 gtgccgctga ccggcgcgat gcggatctgc gcgacccgct gctgcgcggc cggcgaggtc
     5881 acgaccgtct cggagaagta gtcgcggtag gcctgctcga gcgcggcggc gcggacgtag
     5941 tcgcggtagg tcgactcgtc gacgccctgg tcggccagca cctgccgcag gccgccgtcg
     6001 gaggtcgcct cccggcggtc gagaagctcc aggaccgcat agccgcgctc cgtctcgacc
     6061 ggacccacga gatcgccgac ctcggcgtcc gccagcgagt cgaagtactc gccgtaggcg
     6121 gagtcgtccg cctggaacca gccgagctcg ccgccgatgg aggaggtgaa gtcgtcgctg
     6181 acctcggtcg ccacggtcgc gaagtcctca ccgccctcga cccgagcgcg cgccgcctgg
     6241 gccgcctcct cggccgcggc cttctgctcg tcggtcggct cgtcgtccgg ctgcgcgtcg
     6301 tcgggcagcg agtcgaccag gatgaggcgg gcgcgcaccc gctccgggag cgcgtagcgc
     6361 tcggcgagcg cggcgtccac gtcgtcatcg ctgacgctga tccccagctc gtcggccctg
     6421 gcggcgagga cctgctggtc gaccagggac tgggccgcat ccgtgtcgag gctgttgaag
     6481 gccacgctga tctggtcgat ctgctgttgg atcacctggt cccgcgccga cccggtgagc
     6541 tgtgcctgga gctcggtgac ccgcgcgatg gcctccgcgg tgaggatctt cgtgcgctgc
     6601 tcgaggtcgg cgcgatcgaa cgtcgtgtcc cccacggccg ccacgggccg gaagtgcgac
     6661 tcccagtagc cgttccaggc cgctgcgccg aagatgccga gcgccacggc gatggccacg
     6721 gcgaagccga tgatggtcag ctgctgcgtg cggagctcgt cctgccagcg cggtcgatgc
     6781 ttgcggtcga ggacaggtcg ggatcggagg gtcatcggct cctggtcatg cggcggctcg
     6841 gcgagcggcc cgcgcgggcc gcggcggcgt ggcgaggcga catgctagcc gatggaccgc
     6901 cccggcgcac ggtccggatc agtcccggcc gccgcgcagc gcatcggcga tcgaccgcca
     6961 gcgcaggcgc aggacgagga gcatcgcctc ccggacgatg ccgccggtca tcttggacgt
     7021 ccccaccacg cgctcgcgga agacgatggg cacctggacg atcctggcgc cgcgccggtg
     7081 ggcccaccac gtcgtctcga tctggaagcc gtagccggat cccgacgtct cgcgcagccg
     7141 gatggcgtcg atcagctcgc gccgccacgc cttgaagccg ccggtgaggt cgcggtacgg
     7201 cagcagcagc acggtgcgcg cgaagatggt cccgccgcgg ctgatgagct tgcggtacca
     7261 cggccagccg acggtgccgc cgccgcgcat gtagcgcgtg ccgagggcaa ggtcggcgtc
     7321 gcgcatcagc ggggcgagga gccgtggcag gtccgcgggg tcgtggctga agtcggcgtc
     7381 catctggacc acggcgcgcg tctccgggtg ctcgagcacc cagcggaacc cgtcgcggta
     7441 ggccacaccc aacccctcct tgcccgagcg atgcaggacc cgcacgcgcg gctcacccgc
     7501 cgcgatcgtg tcggcaagct cgccggtgcc gtcgggcgag tggtcgtcga cgaccaggac
     7561 cgatgcctcc ggaagggcgg cgaggatcgc atccagcatg ggctcgaggt tgtcgtgctc
     7621 gttgtacgtg ggcaggacga cccacacgcc acggccagcg gcgaggccga ccgacgggag
     7681 gtcggccgcc tcagcggccg acgccgacat acccgacgcc ggcgcggtcg aagcgctccc
     7741 ggtccaggac gttgcggccg tcgacgaaca gctcgagccc cggcagtttg gacggatcga
     7801 ggtcctggta ggcggagtgc gcggcctgca ggatcgccac cttgaccggc tgcgcggagt
     7861 tgaggtcgta cggctcgaag ccgagcgcgc gcaggtggtc gtcgtcgaag aacgggtcgt
     7921 gggcgtagac ctgcgcgccg gccgccagca gctcgtcacg cagccggaag gcggagctga
     7981 aggcgtcctc cttgacgtcg ccgcggtacg ccacccccag gacgaccacc ggcacctcgt
     8041 ccagcgagcc gatccggtcc tcgatgacgt cgaccacgaa ctcgcccatc ccctcgttga
     8101 tctggcgggc cagcggcggg atctgcagct ccggatcgtc gttgaacagg aagtgcgggt
     8161 agaccgggat gcagtggccg ccgacgccga cgcccggctg gtggatgtgg ctgtacggct
     8221 gcgagttcgc ggcgccaatg acctcgctca cgtcgatccc gttgcgctgc gcgaagcggg
     8281 ccagctcgtt ggcgtaggcg atgttgacgt cgcggtacgt cgtctcggcc agcttcgtca
     8341 tctcggcggt ttcggcgttg gtgacggccc acacgtcggt cccctcgtcc aggacggcgc
     8401 ggtagaactc gacggcgcgt cgcgtgctct cctcgctggt gccgcccacg atcttcgggt
     8461 agcgctggag gtccaggaag acgcgaccga ccagcacgcg ttcagggctg aaggccagga
     8521 acaggtcgcg gtccagctcg agacccgagc cgcgcgcgag catcggtccg aagcggttgc
     8581 gcgtcgtgcc gaccggcagc gtcgtctcgt acacgaccag ggcgccgggc ttgacgtgac
     8641 gcgcgatgtc agcggtcgcg gcgtcgatcg gcccgaagtc gatctggcgg tccgcgtcga
     8701 cgaccaccgg gacgatcacg accacgacgt ccgcctcgcg aacgccctcg gcgtcgcgcc
     8761 acgtggtcgc gcgcagccgg ccggcggcgt ggagctccgg gacgcggtcg atgagcgtct
     8821 gctcgtcgcg gtgcggggac tcgccacggt tgatgctgtc gacgatcggg tccgcgacgt
     8881 cgacgccgag cacgtcgtgc ccgcgcgacg cgtactggac cgcgagcggc agcccgatgt
     8941 ggccgaggcc gaccacggcg atcttcacga gagaacctcc tgcagggcgg gaccggcgat
     9001 gacctcgccg ctgagcgcgg attcaaccat cttccgtgcc aggagcagcg cgaccatggc
     9061 gtcgtgcgga tcgaccggcg gcggaccgcc gtcgcgcacc gcccgcgcga actcgcccag
     9121 ctcgaccgcc agcggctcct ggcgccgcac gctgcggcgg accatctcac cttccgagac
     9181 ggaggtgacc tgggcgccga ggccggccgc accacggttg atccacgtgt ccggcgcatc
     9241 ggggttggcg tagaagacca ggtcctggct gatgtagtcg gcgacgtaca tcccgcgctc
     9301 gccggtaact gtcagctggc gcttcttggt cggcgtcagc cagttgatgt ccacgacccc
     9361 gacgacaccg ttgccgaact tcatgatccc gttgaacagg tcctcgtgct cggtgtggat
     9421 ccgccgctcg gtctccgcgt agatgcggat ggggtcgtcg ccgacgaggt agcggatgat
     9481 gtccaggtcg tgcggcccga ggtccacgac gacgccgacg tcgcggatgc gcgccgggaa
     9541 gggccccagg cgctgcgcgc tgatctggaa gatgcggccc agctcgccgg cgtcgaggcg
     9601 tcgcttgagc tcccgaacgg cggggttgaa gcgctcgatg tggccgaccg tcagcagccg
     9661 cccggcggag gccgcagcct cgatcatctc gaccgcctcg gcgcgcgtgg ccgcgatcgg
     9721 cttctcgacc aggacgttga cgccggcgcg caacgcggcc agcgcgaccg gcaggtgcag
     9781 cgacgtgggg gcgacgatgc tgaccaggtc gagctcctcc tcggccagca tccgctcgat
     9841 ctccgcgtag ccgcgcgctc gacggccggc cgtggcacgc tcgaccgcgg cggtgtccgg
     9901 gtcggcgacg gccaccagct ccacgccggc aacggcctcg tcccagacgc ggacgtggtt
     9961 gcggcccatc atcccgagcc cgatgacgcc cgcccgcagc gggcgctgct cgctcatgcg
    10021 cgcgcggcct cggcgggaat cggcccgacc tcttcggcgg tggcgttgac cgcctcgacc
    10081 accgtcgaca cctcatcgtc ggtcagcgcc gggtggaccg ggatgctcag gacctgctcg
    10141 gtgaggcgat cggtcacggg gtacgactgg tcgccgtagc ccagcgccag gaacggcttc
    10201 tgacggtgca cggggatcgg gtagtagatg gcgctgccga cgccacgctc cttcaggcgc
    10261 tcggcgaagg cgtcgcgctg gttcacccgg atcgtgtact ggtggtacac gtgcgtcacg
    10321 cctgggcgca cccatggggt gatcacgccg tgcagctccg cgtcgtagcg cgccgcgatc
    10381 gcgcgccgtc ggtcgttgta gccggcgagc ttcggcagct gggccaggcc gatggccgcg
    10441 gcgatgtccg tcatccggaa gttgtagccg acgacgtcgt ggtggtagcg gaccttcatg
    10501 ccgtgctcgc gcaacaggcg gatgcgctcc gccagctcgg cgtcatcggt cgtgaccatg
    10561 ccgccctcac cggtcgtcat gttcttggtg ggatagaagc tgaacgtgcc cgcgccccac
    10621 gtcccgctcc tgcggccgtc caccgccgcg ccgtgggcct gcgccgcgtc ctcgaccagc
    10681 gcgaggccgt ggcgttccgc gatctcggcg atcgccggca ggtcggccgg ctggccgtag
    10741 aggctgaccg gcatgatcgc cttcgtccgt ggcgtgatgg cggcttcgac ctgtgcgggg
    10801 tccatcgtga agtcgcgctc gtcgatgtcg acgaagaccg gacgcgcccc ggtgtagagg
    10861 atcgagttgg cgctggcgat gaaggtgaag ggcaccgtga tgacctcgtc gccctcgccg
    10921 acgccgtacc cgagcagcgc caggtggagc gccgtcgtgc ccgaactggt ggcgacggca
    10981 tgcccggcgc cgaccatggc ggcgaactcg tcctcgaact ggtggacgcg cggcccctgg
    11041 gccagcgagc ccgatgccat cgcgtcccag acgcgctcct tctcctcctc gcccatctgc
    11101 gggcgcgcga tcggaatcat cgtgcgggtg cctcctccgg catcggcgcc gtgacggcgc
    11161 gtgcgtcgtg tcgttccagc gccgccacga ttcgggcggc ggcgcggccg tcgccgtaga
    11221 gcggcggccg tggcttgtcg cgggacatga agtcgtcgtc accgagcgcg gcgcgcagcg
    11281 cggccggatc gtcgtccacg acccggttcc agccggcggc gaccgtctcg acccactcgg
    11341 tttccgagcg cagcgtgacg caggggacgc ccgacaggta ggcctccttc tgcacgccgc
    11401 ccgagtcggt ggcgatggcg tgggcgcgcg cctcgagcgc caccatctcc agatacccgg
    11461 caggctcgac ggggacgacg ttggacggga gcccgtgcgt gccgcgctcg aggatcgcgc
    11521 gtgtgcgcgg gtggacgggg aagatgaccg gcaggtcgag ctccagcccg gccaggatgc
    11581 gccggaaccg atcggggtgg tcgacgttct cggcgcggtg catggtcaac agcacgtagc
    11641 gatcgtgctc gcgcgcgacg gcggggaggt gctcgtcggc ccgggcggcg gcccaggcgt
    11701 gggcgtcgac catgacgtcg cccgccagct ccgcacggtc ggccagtccc tcgcgcgcca
    11761 ggtgctccat ggccgcctcg gtcggggcga ggagaagctc ggcgagatgg tccgccacga
    11821 tccggttgcg ctcctccggc atgcgcgggt tgaacgagcg caggccggcc tccacgtgcg
    11881 ccagccaggg acgccggccg tcgggatagg ccagcttgca ggtgacgacc gcggcggcca
    11941 gcgtcgagtt cgtgtcgccg tagacgagca cgccatccgg ctcgtgctcg agcaccacgg
    12001 gctcgaggcg ccgcatcatc tcgccggtca tcgcgccgtg cgagccgctg ccgacctcca
    12061 ggttgacgtc cggctccggg atctcgaggt cgcggaagaa ggcggcgctc atcgcctcgt
    12121 cgtagtgctg gccggtgtgg acgaggatct cggtgtgggc ctcgcgcagc gcacggctga
    12181 ccggcgccgc cttcacgaac tgcggtcggg cgccgaccac ggtgaggacc ttcaccccgc
    12241 ggccccggat gcgctcggcg ggaacgcctc gccgcagcgc gggcaggttc cgcggaaggg
    12301 ctctccgtcc tccgtgtcgc gcagcggctg gccgcacggg caggcgctcc cgagccggcg
    12361 cgccgggttg ccgaccacca gctcgtagtc cgccacgtcg cgcgtgacca cggcacccga
    12421 gccgaccatg gcccagcgcc cgaccgtcac gccgggcagg atcgtcgagt tcgcgcccag
    12481 ggccgcgccg tggcgcaccc ggatcggcgc cacctcccag tccgcatcgg tcttgagcga
    12541 cccgtccggg ttgatggcgc gcgggatgag gtcgttggtg aagcacacgt gcggaccgat
    12601 gaacacgccg tcctcgacgg tcacgccgtg gtagatgctc acgttgttct ggaccttgac
    12661 gtcgtcgccg aggacgacgt cgaagtcgac gtaggcgttc ttgccgatga tgcagcgcga
    12721 cccgatgcgg gcgcgctcac gcacctgcgc ctggttccag atgctggtgc cggggccgat
    12781 ctccgcctcc ggcgagacgt ccgcggtcgg gtggatgcgc acctgcgcac gctcggccat
    12841 gtgatctcct tggggcccga ccattctagc cggggatcag ccctggtcga actccatgac
    12901 cacgagcagg cgctcggccg aatgcacgtc gaatgccggg cgcacgaacg cggtctgggt
    12961 cagggcgttc gcatcggtct cgaccgcctg gacggtcccg atcagcaggc cgccggggaa
    13021 gcgcgacgcc tcctcgtccg tggtcaggcc cgcggtcacg atggcgtcgc cgacggcgac
    13081 cagctcggtc accgggatct gggtcatcac cagctgtccg ccgggctggc cgcgcacctc
    13141 gcccagggca cggctggcct ggtcgagcgc gatcacccgc gagcgggtgt cgaccacgaa
    13201 ccgcacggtc gccgtgtcgt ccgtgaccgc cacgatggtc ccggccagcg cgccgaccgc
    13261 atcggcgttg ccgacgaccg gcatcccgac gcgaatgccg tcccgcgaac cggcgtcgac
    13321 gccgacctcc caggcaaagt tggacgggtc gcggcggatg acccggacgg gcagcgtcgt
    13381 catctgcagc gcgtcgcgaa tgcccaacag gtcccgcagc tcggcgttct cgcgcgccgc
    13441 ctccgtcagc tcggcgatgc gctgctccgc cccgttgagg tcgcgacgca gctccgcgtt
    13501 ctcgccgcgc agccggtcga tctcgccgat ggtgccgaac agcccggcca cgccggcccc
    13561 gacgccgccg atggccgacc gcacggggtc cagggcgcgc gtcgtcacgt gctgcagcgt
    13621 gcgcgccggc tccgtggagc tggcgagcag catgaggagg ctggcgatgg tgaaggccgc
    13681 gaaccacagc ccggtgcggc tgcggcggtg agggctggcc ggcccgtaca tcgaccgtgc
    13741 gtcaggggcg ccgacgtata tcgcgatata cggcggagcg tgggacgccc atcagcgcgg
    13801 ggcgcggtgg tactgctcgc tgaccag
//
LOCUS       DASTYP010000016        16392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1494542, whole genome shotgun sequence.
ACCESSION   DASTYP010000016 DASTYP010000000
VERSION     DASTYP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494542"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VFV59_00750"
     CDS             <1..1134
                     /locus_tag="VFV59_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5324395.1"
                     /translation="KLAEGAIRGMFEYGVADPYSGYMPPQDYAQALGDLSGSFGGIGA
                     EMAVKNVDNPADLAACQQFDERCRLVVVAPLADSPAEAAGIQAGDLVLAVDGVSVDGT
                     TMSDQIAHIRGEAGTEVTLTIQRNGGDPFDVTITRADITLQEVETRLIDGHVGYIALN
                     GFSAPAAEQFKAGLQDLLDQGADQIVFDLRDNPGGYIDAAQKIASQFVDSGLIFTQES
                     AGDEVKEYRSTGDGIATDANLPVVVLVNGGSASASEIVAAALKESGRATLVGEPTFGK
                     NTVQVWGRLENGGGVRITVSRWFTPDHHSVAPDGVQPDVPAARTAETPPEEDPILDAA
                     LAYLADQPVAGDGSGSPAPSGSPSAFMPDAAPVVGIAALRPVC"
     gene            1307..1771
                     /gene="smpB"
                     /locus_tag="VFV59_00755"
     CDS             1307..1771
                     /gene="smpB"
                     /locus_tag="VFV59_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU5324396.1"
                     /translation="MPDETIAVNRRARHEYAIEDTLEAGLVLTGTEIKSIRAHKVNIA
                     EAYVRIDHGEAWLIGAHIAEYAHGNRYNHPPTRTRKLLLHRTQIDELLGLQSAKGLTI
                     VPLRLYLRGGNAKLEIGIARGKKAHDKRRTIAERDMRRELERESKAAGRIKL"
     gene            2063..2430
                     /gene="ssrA"
                     /locus_tag="VFV59_00760"
     tmRNA           2063..2430
                     /gene="ssrA"
                     /locus_tag="VFV59_00760"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2624..3082
                     /locus_tag="VFV59_00765"
     CDS             2624..3082
                     /locus_tag="VFV59_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324397.1"
                     /translation="MDWSRLSSNDKLAVYGAVAVIVGGLVGLGAAAYGVVAVLAAVGA
                     LLVVFLPQLSPATNLPGSKGSLLLVLGAIAAIILVLGLLNVLGALGFLLQFAAISTLF
                     YLIAVAGSVLMAWAGWQAFQAEGGQLRLGTTARDTEGHGTPRDEDAAPRS"
     gene            complement(3084..3473)
                     /locus_tag="VFV59_00770"
     CDS             complement(3084..3473)
                     /locus_tag="VFV59_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324398.1"
                     /translation="MAPDGPWIGFAPALDDTYVLVIGGAGASRREPADADDLLSLALA
                     YFEDGLEAPPEELAATHADIGALVRHLVEVETDASRRASLTEAVDAIDDGLAPDVVVG
                     RLVACLNGEEEAVARLSRRAAAALGGS"
     gene            complement(3541..4644)
                     /gene="jag"
                     /locus_tag="VFV59_00775"
     CDS             complement(3541..4644)
                     /gene="jag"
                     /locus_tag="VFV59_00775"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEU5324399.1"
                     /translation="MTFREFTGKNVEEAIRSAMKEFSADLSDLDIEILSQGKRGMLGV
                     GGEEARILAAPKSAIAAAETVRAEAPAEAPAERPARPAARGRGRGRSVTPDEVPAEAP
                     SAAEPVGSEPAEEATPSPAAEVGEDAPAEAPFAAASDEASLGEDRGERRTRGGRGRGG
                     RGRNGREREYAGREAREPRESREHREPREARGDRPGREPAPFVSGAPVEELSEAERST
                     LETAKTVLEEMLDLMGLKGTVEIASGGETARLNVKGEDLGALIGRRGEKLASLQHIVN
                     LIVGRREGQHHRIAVDVENYRGRREEQLRDVAERAAKRVIQTGKIIQLEAMPAVERRI
                     VHMALVDNPKVRTQSVGVEPNRRIVVLPATQAR"
     gene            complement(4641..5786)
                     /locus_tag="VFV59_00780"
     CDS             complement(4641..5786)
                     /locus_tag="VFV59_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEU5324400.1"
                     /translation="MTPLIGAGFLGIEHFTPPWDVFFLPLFNLLVALYHIPFFDFALA
                     IIIFTVLIRTVLAPLFVRQIRSQKEMQRMQPLIKEVQRKHKGNRQKIYEETQALYREH
                     GVNPAAGCLPVLLQLPILFALYQALIRASGVIQNFTPGNDQQRAAFEVLQNNGLVTAL
                     EKANHYQVAVSGQCNLPEFSTLPQFLPINCQLIDPIKLKEPVDTTIGWLFNLNLADID
                     RTFALVLGGENGFAISALAILAAILQFVQVKMTSPRPNPDDPSSGMTSTMTYTFPLLT
                     VVWGGIFPSGLILYWIVYTTYLVVQQYLIMGWGNLFPLFGWTPGWARSTDADSARSSR
                     PARLSSDDAPSTSQPTPPSAPAQGGGRGGPRPGPRKQGGAKRRGRKR"
     gene            complement(5802..6008)
                     /gene="yidD"
                     /locus_tag="VFV59_00785"
     CDS             complement(5802..6008)
                     /gene="yidD"
                     /locus_tag="VFV59_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU5324401.1"
                     /translation="MKKIGLGLIIAYQKLTRGMPPSCRYSPSCSVYTYQAIEKYGLLR
                     GSWMGAKRIGRCNPFHPGGYDPVR"
     gene            complement(6005..6340)
                     /gene="rnpA"
                     /locus_tag="VFV59_00790"
     CDS             complement(6005..6340)
                     /gene="rnpA"
                     /locus_tag="VFV59_00790"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU5324402.1"
                     /translation="MPALPTIRRRADFEAIGRRGSRRSTPLLVLRSLRTDRADTRIGL
                     STPRTLGGAVHRNRVRRRLRELLRRRLAGIGPGWDLLLIARPPAANASFAELGAAIDA
                     LLRRPLDGK"
     gene            complement(6360..6494)
                     /gene="rpmH"
                     /locus_tag="VFV59_00795"
     CDS             complement(6360..6494)
                     /gene="rpmH"
                     /locus_tag="VFV59_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5324403.1"
                     /translation="MKRTYQPKKRARKREHGFMARMATKGGRRVLARRRAKGRKRLSA
                     "
     gene            7013..8356
                     /gene="dnaA"
                     /locus_tag="VFV59_00800"
     CDS             7013..8356
                     /gene="dnaA"
                     /locus_tag="VFV59_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5324404.1"
                     /translation="MDAKQVWRAALGELQVSLSPANFETWLKDTQLVEMEDNRFRISA
                     PNGFARDWLDNRYRPLISQTLARVVGGSVQVEFVVADAAGPVPEQVDEEAQVTMPPLS
                     AGGNAVNLNSRYTFSNFIVGSANRLAHAAALSVAERPGHAYNPLFLYGGVGLGKTHLM
                     HAIGNAVAAKFPRKRILYATSEKFTNDFINSIREQKMEDFRSRYRRIDVLLIDDIQFI
                     AERERTQEEFFHTFNAIHEAGKQVVLSSDRPPKAITTLEERLRSRFEWGLIADLTPPD
                     LETRIAILRSKAEDQLHLIPSEVIEFIARKVVSNVRELEGALNRVVAYASMSGMPVNI
                     ELASAVLSNVMYNPRKKSITPQRIVRAVADYYGVNLDQLRSSKRDRAIVVPRQIAMFL
                     IREETDISLLRIGAELGGRDHSTVLHACDKINRELNENDELRREISAVREMIYSE"
     gene            8724..9860
                     /gene="dnaN"
                     /locus_tag="VFV59_00805"
     CDS             8724..9860
                     /gene="dnaN"
                     /locus_tag="VFV59_00805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5324405.1"
                     /translation="MNVSVMQENLVRGLQTVSRAVSTRATLPVLANVLLKTENSGVKL
                     TATNLEIGINCWVPGKVTEEGEITVPARLLTDLVASLPNQRIDLVLSPKDRTLKLTCG
                     SNRSSIKGIEADEFPVVAAIGESPATSVDARVLREALGEVVFAAATDESRPILTGVLT
                     RLSGDTMTLAAADNYRIAVRTVKLDRPVSPDVTIVVPGRSYAELMRIMPDAEQPIEIT
                     VTPNKSQVLFHVDGIDLVSRLIEGQFPNYEPVIPTSHTSRAVLDREAFLSGTRRASIF
                     ARDSANIVKIELGGDGGDGEVVGVSITAHAADVGDNADTVEASVEGSPTTIAFNARYL
                     IDVLSNLGSDEAALELSGPLAPGVIRGIGKDDYVHVIMPVRTAS"
     gene            9912..11081
                     /locus_tag="VFV59_00810"
     CDS             9912..11081
                     /locus_tag="VFV59_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU5324406.1"
                     /translation="MELTRLSLEDFRSYAAAELRPDPGLTVVAAPNGTGKTNLLEAIH
                     VAITGRSHRAAVDAELVRHGRPFARVRLELDRGTAEGGATIELVLPGTEAPAGIRKRL
                     TVNGVPRRASTVSETARAVLFRPEEMLLLVGSPSDRRRFLDGILAQRDRRAARDLADL
                     ARVLAQRNALLRAIRAEEAPLDSLAFWDEQLAQVGARVMRERLAVVRELGERIGPLHD
                     AVATSGERGADVRLAYLDGLKEAWPERADPRERLPSVEELTAAHRRRITDVRTKEAWN
                     GVSLVGPQRDDLRAELSGRDVAAHASRGQQRTIILALKLAETDLLGADGPRPIVLLDD
                     VFSELDPERSTRLSELLAGLGQVLVTTADPGTLDAARARGGAIWRIRDGRLEPAP"
     gene            11078..11428
                     /locus_tag="VFV59_00815"
     CDS             11078..11428
                     /locus_tag="VFV59_00815"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEU5324407.1"
                     /translation="MSGREEAGAAVQRALAECMGPGAAAQLAVAQVRVAWAEVVEEAR
                     LQRGPLTSRVSRVTGGTAHVEASDPMLAQELTLRSEALVWAVNERMKGRPGATIVLHG
                     LAVSVGRGGARPTL"
     gene            11466..13691
                     /locus_tag="VFV59_00820"
     CDS             11466..13691
                     /locus_tag="VFV59_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324408.1"
                     /translation="MTNRFTTRIGLPSADERPAGAHDVVAFREPTTGSELRTKGSIFL
                     LAQLTSPTPALERAVTEALEAIQRDYYYDLSGGVLVALARALRAANRRLYHQRRRLGI
                     PRRSGVSVVALVVRGREAHVAKLGPASAVVVREARMYEVPPPPAVEDEDPRVRRRRVA
                     GTLGEALEIEPYTWHGPLAAGDRLALVSRHFAHTVGVEELKSALSGMRPSAAVEHLVQ
                     LFGIRGGSGSDGLLVVGIEELPATATTQHLEPVRPAEPFAGLPDQSPVPLADALGRGL
                     HRAGDAAEAAKAALGRGLLTLVSWILAFVPRRRPEYPRTIPRTAEREAGRRRRIGLAG
                     MVVVAALLAVGSTVAAVPSVRPTDAIPRATVAREAIVEAVDLLDLVGQRVDGADLVDR
                     DPERATDLLADARAALDRAAGAGVRHDQLRPLRSRVDDRLDAIYRVTRIAAPDTVAAL
                     GEDYDDLEPLHMVAASDGSLWVLDGGRGRVIRVDPVDGSSTVVYRAGQALESGDVPGD
                     PWLMATAATDVVVIDRARQAWRMDLTERIPRPMVLAGVEALGSDTTLLGALQHRPPLE
                     IFNLYAVDATTGQVLRWTPPAVIPVNYPDPPEPFLTDEPDLDPRDARDLRVDVNAWLL
                     HAGTVTRVDFGSPRDQVEYSLDPPPDDAVRPTLDYRLLDGATVGDRELLYVYDAASSR
                     IIAFQRADGAFVRQWLAPADGPAAGSLDEVIGLSVTSVADGPPTAYLLTPDGVVRLVL
                     E"
     gene            13711..14268
                     /locus_tag="VFV59_00825"
     CDS             13711..14268
                     /locus_tag="VFV59_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5324409.1"
                     /translation="MADQPFGRVRLVEMVVESVRVHMLSSQHVVILKESERDRYLPIW
                     IGSGEANAIAMRLQGLSAERPLTHDLLVSVIGALNSSVSRVVVTHVTDGTFHARLYLE
                     TANGDEAEVDSRTSDAIAVAVRTGSTIYVDERVLDEAGVEPDSTEETTPEEDERLAVF
                     REFVNSLDVDLPERGPEAGPPPEES"
     gene            complement(14265..15038)
                     /locus_tag="VFV59_00830"
     CDS             complement(14265..15038)
                     /locus_tag="VFV59_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5324410.1"
                     /translation="MTDPERPRLTFFFPAYNEEENVAETVRRALDEVGPLVDDSIEVL
                     VVDDGSTDRTPELADALAAADPRVRVHHQPNRGYGGALRAGFDNARGELIGFSDGDLQ
                     FDLREFSRLLERLDDPKRGRFDAVIGWRIKRRDPPHRIVIAKTYNAIVSLAFGLRVRD
                     IDCAMKVFRREVFDGLNLTAESPFLSAELLIKLKARGERMAQVGVTHYPRAAGTNTGA
                     SFRKILRTFRDIGRLRWALWTRRSETLGPVRAREAGGSA"
     gene            complement(15035..>16392)
                     /locus_tag="VFV59_00835"
     CDS             complement(15035..>16392)
                     /locus_tag="VFV59_00835"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU5324411.1"
                     /translation="GEVAWRMNLLSAVCVALAAGLVTLIAGHLVREPSRSVAAVAAGI
                     GGAAFAFASEPWENSSRADVHAITVFFVALVVWLLFAWGAAERAGARRAGGWLIGAAF
                     AFGLGMGVHPLVGLTAFGIAAWLLVVHGHLWRRWRLVLACAAVLALGIGSYAFIWIRA
                     NIDPAPPLFYAHPDTWERFRYLVFAEQFKSLFHDFRDPLAELSSKWVDAERVLSAQFI
                     GPGWLLVAGGAAVLAVRRPGVLAFLGLVVVANVLYSMNFRDGDIDRYYMTTVVATAPL
                     LAVAVGAIATAGARAVGEMGLRNAMPRRVRRRVTAVVGGTVLAASMLLPAVGLVTGYA
                     SHDQSTNREADQWVASVYEQLPPNAVIISWWSYSTPLWYHRWIDGARPDVTIIDERNI
                     LDDGYRTMRNAIASFYGERPVYVVPPDWEWRRLDRDWEYRNVSTYRGYTDLLLIEGRR
                     R"
BASE COUNT         2463 a         5854 c         5735 g         2340 t
ORIGIN      
        1 aagctggccg agggcgccat ccgcggcatg ttcgagtacg gcgtcgccga tccgtactcc
       61 ggctacatgc caccgcagga ctacgcgcag gcgctcggcg acctgtccgg ctcgttcggc
      121 ggcatcggcg cggagatggc cgtcaagaac gtggacaacc ccgctgacct ggccgcgtgc
      181 cagcagttcg acgagaggtg ccgcctggtc gtggtggcgc cgctggccga ctctccggcc
      241 gaggccgccg gcatccaggc cggcgacctg gtcctggccg tcgacggcgt ctcggtggac
      301 ggcacgacca tgtccgacca gatcgcccac atccgcggcg aggcgggcac cgaggtgacg
      361 ctcaccatcc agcgcaacgg cggcgatccg ttcgacgtga ccatcacgcg ggccgacatc
      421 acgctgcagg aggtcgagac acgcctgatc gacgggcatg tcggctacat cgcgctgaac
      481 gggttctcgg ccccggccgc cgagcagttc aaggccggcc tgcaggacct gctcgaccag
      541 ggcgcggacc agatcgtctt cgacctgcgt gacaacccgg gcggctacat cgacgccgca
      601 cagaagatcg cgagccagtt cgtcgactcc ggtctgatct tcacccagga gtcggccggc
      661 gacgaggtga aggagtaccg atccaccggc gacggcatcg ccaccgacgc gaacctgccg
      721 gtcgtcgtcc tcgtcaacgg cggatcggcc tccgcctccg agatcgtcgc ggcggcgctc
      781 aaggagtccg gccgggccac gctggtgggg gagccgacgt tcggcaagaa cacggtccag
      841 gtgtggggcc ggctcgagaa cggtggcggg gtgcgcatca ccgtgagccg ctggttcacg
      901 ccggatcacc acagcgtcgc gcccgacggc gtccaacccg acgtgccggc cgcacggacc
      961 gccgagacgc cacccgagga ggatccgatc ctggacgccg cactggcgta cctggccgac
     1021 cagccggtcg ccggcgatgg cagcggcagc ccggcaccgt ccggatcgcc ctcggcgttc
     1081 atgccggatg ccgctccggt agtcgggatt gccgcgcttc gacccgtctg ctagcatcgc
     1141 cggcaacgaa aggaggtggt gtgcaggtga gtagttccaa gcacgccacc accgaggcgg
     1201 tgcgataaca cagcttcgcg tggtgtagcg aaggggagag ccgaccgaaa cggtcggctc
     1261 tttccgtgtc cgcccccctt cacgacaaca ggaccgatcc agaccgatgc ccgacgagac
     1321 gatcgccgtc aaccggcgcg cgcgccacga gtacgccatc gaggacacgc tcgaggcggg
     1381 cctcgtgctg accggcaccg agatcaagag catccgggcg cacaaggtca acatcgccga
     1441 ggcgtacgtg cggatcgatc acggcgaggc ctggctcatc ggcgcgcaca tcgccgagta
     1501 cgcgcacggg aaccgctaca accacccgcc gacgcgcacg cgcaagctgc tcctgcaccg
     1561 cacccagatc gacgagctcc tcggcctgca gtcggccaag gggctgacga tcgtgccgct
     1621 gcgcctgtac ctgcgcggcg ggaacgccaa gctggagatc gggatcgcgc gcggcaagaa
     1681 ggcgcacgac aagcgccgca ccatcgcgga gcgcgacatg cggcgcgaac tcgagcgcga
     1741 gtcgaaggcg gcgggccgca tcaagctctg attccggggg cgtcttggtc ggcctgggtt
     1801 cgcgcggcgt ggcgtgcggt cggcctgaag gctccagatt cacatgacct gtgtatccgc
     1861 ttcggtgcac gtgcctggag gcgccccgga gcccgcagac tcacacgacc cgtgaagaag
     1921 gaccgttcca ttgggtggac atcgccttcg gggccgccgc gcgcacgcct ggtgtgaatt
     1981 ggagcaagtc ggcggagcga ggtctcctag aacaggtgtt cacatcggcg catcggtctg
     2041 ctagaatggc ggcctggttc gtggggatgt caggtttcga cagggctcgt gaaccgggaa
     2101 tcgcaggtcg aggttgctcg caggcctcgt aaaacagcgg gcaaaccagt aactggcaac
     2161 gaacgtcagt tcgctctcgc tgcttagtca gtgagcactg acgcggcccc acctccccgg
     2221 gccgcgaagg tgtcatcagg ggaggtgcgg cgtcaccgac tccggtgagt ggcgtcaaag
     2281 agccgagagg cagcaccgga tagtcggtcc gataccccgc cggtcgggga tcgggcaacg
     2341 gcgaaacccc aagtaggccg gaaatacctg taggaagttc tcgggaatct ggttctggac
     2401 ggggagttcg attctccccc atctccacca ccccactccg tcctcgcgcc agcgcgcact
     2461 tcttctccgc gagcgtctcc ggcgggatgg aggccgcgct gccggtgatg aatccaccgg
     2521 atccgtgatc ctgactcggt ggtgtctcgg cgcgccaccc ggctgtgcta cggtccccag
     2581 cggctcaggt gggggatgtc gctagcggag ggtggtctcg ctcatggatt ggtcccgtct
     2641 cagttcgaac gacaagctgg cggtgtacgg tgccgtcgcc gtgatcgtgg gcggcctggt
     2701 ggggctcggc gcggcagcgt acggcgtcgt cgcggtactc gcggcggtcg gcgcgctgtt
     2761 ggtcgtgttc ctcccacagc tctcaccggc gacgaacctg ccgggatcga agggcagcct
     2821 gctgctcgtg cttggcgcga tcgcagcgat catcctcgtc ctcggactcc tgaacgtcct
     2881 cggcgcactc ggcttcctgc tgcagttcgc cgcgatctcc accctcttct acctgatcgc
     2941 ggtcgcgggc agcgtgctca tggcgtgggc gggctggcag gccttccagg ccgagggcgg
     3001 ccagctccgg cttgggacga ccgctcgcga caccgagggc cacggcaccc cgcgcgacga
     3061 ggacgcggca ccccggtcct gactcaggag ccgccgagcg cggccgccgc ccggcgggag
     3121 aggcgggcca ccgcctcctc ctcgccgttg aggcacgcca ccaggcggcc cacgaccaca
     3181 tcgggcgcca ggccgtcgtc gatggcgtcg acggcctccg tcaggctggc gcgccgagac
     3241 gcatcggtct caacctccac caggtggcgg accagcgcac cgatgtcggc gtgcgtggcc
     3301 gccagctcct cgggcggggc ctccagtccg tcctcgaagt aggcgagggc caggctcagc
     3361 aggtcatcag cgtcggccgg ctcacgacgg ctggcgcccg cgccgccgat gacgagcacg
     3421 taggtgtcat cgagcgccgg ggcgaagccg atccacggtc catcgggcgc caaccgcccg
     3481 acagcacacg ccgcgacgtc caccgccgcg cccccgcccc ccgcagcgcc gccgggcgcc
     3541 ctagcgcgcc tgggtcgccg gaagcaccac gatccggcgg ttcggctcga cgccgacgct
     3601 ctgcgtccgc accttcgggt tgtccaccaa cgccatgtgg acgatccggc gctcgaccgc
     3661 gggcatcgcc tcgagctgga tgatcttgcc ggtctggatg acccgcttcg cggcgcgctc
     3721 ggccacgtcg cgcagctgct cctcacgccg gccgcggtag ttctcgacgt ccacggcgat
     3781 gcggtggtgc tgtccctcgc ggcgccccac gatcaggttg acgatgtgct gcaggctggc
     3841 gagcttctcg ccccgacgcc cgatcagcgc accgaggtcc tcgcccttga cgttgagacg
     3901 agccgtctcg ccgcccgatg cgatctcgac cgtgcccttc agtcccatca ggtcgagcat
     3961 ctcctcgagg acggtcttgg ccgtctcgag cgtcgagcgc tccgcctccg agagctcctc
     4021 cacgggagcg cccgacacga acggcgcggg ttcgcggccg ggtcggtcgc cacgcgcctc
     4081 gcgcggctcg cggtgctcgc gggactcccg cggctcgcga gcctcacggc ccgcgtactc
     4141 gcgctcccgg ccgttgcggc cacggccacc gcggccacga ccgccgcgcg tgcggcgctc
     4201 gccacgatcc tcgccgaggc tggcctcgtc ggacgcggcg gcgaacggcg cctcggctgg
     4261 ggcgtcctcg ccgacctcgg cggcgggtga cggcgtcgcc tcctcggctg gctcggaccc
     4321 aacgggctcg gcagctgacg gtgcctccgc gggcacctcg tcgggcgtga cggagcggcc
     4381 gcgaccgcgg ccgcgcgcag cgggacgggc cggccgctcg gcgggtgcct cggcgggcgc
     4441 ctccgcgcga acggtctcgg cggcagcgat ggcggacttg ggggcggcca ggatgcgcgc
     4501 ctcctcgccg ccgacgccca gcatgccgcg cttgccctgg gacaggatct cgatgtcgag
     4561 atcggacaga tccgcgctga attccttcat ggcgctgcgg atcgcctcct cgacgttctt
     4621 accggtgaac tcgcggaagg tcatctcttt cggcctcgtc gcttggcgcc gccctgcttc
     4681 ctggggcctg gccgcggtcc accgcggcct cctccctggg cgggggcgct ggggggagtg
     4741 ggctgggacg tcgagggcgc gtcgtcgctc gagaggcgcg ctgggcgcga gctgcgcgcg
     4801 gagtccgcat cggtggacct ggcccagccg ggcgtccagc cgaacagcgg gaagaggttg
     4861 ccccaaccca tgatgaggta ctgctgcacc acgaggtacg tggtgtagac gatccagtag
     4921 aggatcaggc cgctcgggaa gatcccaccc cacacgacgg tcagcaacgg gaacgtgtac
     4981 gtcatcgtgc tggtcatgcc ggaggacggg tcgtccgggt tcggccgcgg gctcgtcatc
     5041 ttgacctgca cgaactgcag gatggccgcg aggatcgcga gcgcactgat ggcgaacccg
     5101 ttctccccgc caaggacgag cgcgaacgtg cggtcgatgt cggcgaggtt caggttgaac
     5161 agccagccga tggtcgtgtc gaccggctcc ttgagcttga tcgggtcgat cagctggcag
     5221 ttgatgggga ggaactgcgg gagcgtgctg aactccggca ggttgcactg gccgctgacg
     5281 gcgacctggt agtggttggc cttctccagc gcggtcacca gaccgttgtt ctggaggacc
     5341 tcgaaggccg cccgctgctg atcgttgccc ggcgtgaagt tctggatcac gcccgaggcg
     5401 cggatgagcg cctggtagag ggcgaacagg atcggcagct ggagcaggac cggcaggcag
     5461 cccgcggccg ggttgacgcc gtgctcgcgg tagagcgcct gcgtctcctc gtagatcttc
     5521 tgccggttcc ccttgtgctt gcgctgcacc tccttgatca ggggctgcat gcgctgcatc
     5581 tccttctgcg agcggatctg ccgcacgaag aggggagcca gcacggtcct gatcaggacg
     5641 gtgaagatga tgatcgccag cgcgaagtcg aagaagggga tgtggtagag cgcgacgagg
     5701 aggttgaaga ggggcaggaa gaagacgtcc cagggcggcg tgaagtgctc gatgcccagg
     5761 aatccggcgc cgatgagggg ggtcaacgtc ttctcagctc tctagcggac cgggtcgtag
     5821 ccgcccgggt ggaacgggtt gcagcgaccg atgcgcttcg cgcccatcca cgagccgcgc
     5881 agcagcccgt acttctcgat cgcctggtag gtgtacaccg agcacgaggg tgagtagcga
     5941 cagctcgggg gcatcccgcg ggtgagcttc tggtacgcga tgatcagtcc cagtccgatc
     6001 ttcttcattt cccatccagc gggcgtcgca gcagcgcgtc gatcgccgcg ccgagctctg
     6061 cgaagctcgc gttcgccgcg ggaggcctcg cgatcagcag gaggtcccat ccgggaccga
     6121 tgcccgccag tcgtcgtcga agcagctctc gcagccggcg ccgcacccga ttccggtgca
     6181 cggcacctcc gagtgtccgt ggcgtcgaca gcccgattcg cgtgtccgcc cggtcggtcc
     6241 tcaacgatcg gaggacgagg agcggggtcg aacgtctgga gccgcgacgg ccgatcgcct
     6301 cgaagtcggc ccggcgccga atggtcggca gcgcaggcat cgtacccgag actcggacgt
     6361 caggcgctga gccgcttgcg ccccttggcg cgacgacgag cgagcacgcg ccgaccgccc
     6421 ttcgtggcca tgcgggccat gaagccgtgc tcgcgcttcc gcgcgcgctt cttgggctga
     6481 taggtcctct tcatgggtgc gtgccgttcc tcgttggtga cttggcgttc cggaccacaa
     6541 acacacgaac ggctggcgtt ccgccagcct gcgcgcgatc tcggggtggc ccgttcgctc
     6601 tcggcgggat tctagtctcg cgcggcacct cggtcaaacg gcggagggcc tccgttccgg
     6661 ccctccgcag gacccgcgac atcggtccgt tcactgaccc atccatcggt caccgtgcac
     6721 cggccacccc caatccatgc cgggcagccg atgcgtcggt caacccagcg gggcggcggt
     6781 cgcgaacagc tctccggccg agcgtcgtgg ctctgcggag ggaccgaaaa gggcccgaac
     6841 ggcccattcc gacaggacgt ctcgatccgt tgcccggccg attcgggcgc tgctatagtg
     6901 gcccgcaccc ccgccccgga ccgcccgatt cgacgtccga tctcgggtga ggaggacgga
     6961 ctcatcagcc cacgcggcgg cgccgcgaac cggttcggag aaccacgttt tgatggatgc
     7021 gaagcaggtg tggcgcgccg ccctcggcga gctgcaggtc tccctctcac cggcgaactt
     7081 cgagacctgg ttgaaggaca cgcagctcgt cgagatggag gacaaccgct tccgcatctc
     7141 cgccccgaac ggcttcgcgc gggactggct cgacaaccgg taccgcccgc tgatcagcca
     7201 gaccctggcc cgggtcgtcg gcggatcggt ccaggtggag ttcgtcgtgg ccgatgcggc
     7261 cgggccggtc ccggagcagg tcgacgagga ggcgcaggtc accatgccgc ccctctcggc
     7321 cggcggcaac gcggtcaacc tgaacagccg ctacaccttc agcaacttca tcgtcgggtc
     7381 ggcgaaccgc ctggcgcatg ccgccgccct ctccgtcgcc gagcgccccg gccatgccta
     7441 caacccgctc ttcctgtacg gcggcgtcgg cctgggcaag acccacctga tgcatgccat
     7501 cggcaacgcc gtggcggcca agttcccgcg caagcggatc ctgtacgcca ccagcgagaa
     7561 gttcaccaac gacttcatca actccatccg cgagcagaag atggaggact tccgcagccg
     7621 ataccggcgc atcgacgtcc tcctcatcga cgacatccag ttcatcgccg agcgggagcg
     7681 gacgcaggag gagttcttcc acaccttcaa cgccatccac gaggccggca agcaagtcgt
     7741 cctcagctcg gaccggccgc cgaaggcgat caccacgctg gaggagcgcc tccgcagccg
     7801 cttcgaatgg gggctcatcg ccgacctgac cccaccggac ctcgagacca ggatcgccat
     7861 cctgcgatcg aaggcggagg accagctgca cctcatcccg tccgaggtca tcgagttcat
     7921 cgcccgcaag gtggtgagca acgtccgcga gctcgagggc gccctgaacc gggtcgtcgc
     7981 ctacgcgagc atgagcggca tgccggtgaa catcgagctc gcgtcggccg tgctcagcaa
     8041 cgtgatgtac aacccgcgga agaagtcgat cacgccgcag cgcatcgtgc gcgccgtcgc
     8101 cgactactac ggggtgaacc tggaccagct gcgctcctcc aagcgcgacc gcgccatcgt
     8161 ggtgccacgc cagatcgcca tgttcctcat ccgcgaggag acggacatca gcctcctgcg
     8221 catcggtgcg gagctgggcg gccgcgacca ctcgaccgtg ctgcacgcct gcgacaagat
     8281 caatcgcgag ctcaacgaga acgacgagct gcggcgcgag atcagtgccg tgcgggagat
     8341 gatctactcc gagtaggggg tggtgccttc cggcaccgat gcatcggtcg tcgtggataa
     8401 gtggccggtt ctcgtggata tctcggcccc gatgtggaca atccgcacac ccatcgggcc
     8461 gggcgggtac gctccatcca ccgattcggg cgatccatcc gccatccatc cccgccgcgg
     8521 gggccggaca tccacggccg cgcgagggtt atccgcaggg ggtcgcacgc tcggaccacg
     8581 ctcggcgaag gagcctcgtc cacaccgtcc acacgcctac gaagggacga cgacggatga
     8641 gtattcttct gaagaaccga tggagtaccc agatcgtttc gtggatatcg ccacgcgatc
     8701 catgaacgcg cagtgaggac cgcatgaacg tctcagtgat gcaggagaac ctggtccgcg
     8761 ggcttcagac cgtgagccgc gcggtgagca cccgggccac cctgccggtg ctggcgaacg
     8821 tgctactcaa gaccgagaac tcgggcgtca agcttaccgc caccaacctg gagatcggca
     8881 tcaactgctg ggtcccgggc aaggtcactg aggagggcga gatcaccgtg ccggcccgcc
     8941 tgctgacgga cctcgtggcc tcgctgccga accagcgcat cgacctcgtg ctgtcaccga
     9001 aggatcggac gctgaagctc acgtgcggca gcaatcggtc cagcatcaag ggcatcgagg
     9061 ccgatgagtt ccccgtggtg gccgccatcg gcgagtcgcc ggcgaccagc gtggacgccc
     9121 gagtgctgcg cgaggccctg ggcgaggtgg tcttcgccgc ggcgaccgac gagagccgac
     9181 cgatcctcac cggcgtgctg accaggctgt cgggcgacac gatgaccctg gccgcggccg
     9241 acaactaccg catcgcggtc cggaccgtga agctcgaccg gccggtctcg ccggacgtga
     9301 cgatcgtcgt gccgggacgc tcctacgccg agctgatgcg gatcatgccg gatgccgagc
     9361 agccgatcga gatcaccgtc acccccaaca agagccaggt cctcttccac gtcgacggca
     9421 tcgacctggt cagccggctg atcgagggcc agttcccgaa ctacgagccg gtcatcccga
     9481 cctcgcacac ctcacgtgcc gtgctcgacc gcgaggcctt cctgtccggg acgcgccgcg
     9541 ccagcatctt cgcccgcgac agcgccaaca tcgtgaagat cgagctgggc ggcgacggcg
     9601 gcgacggcga agtggtcggc gtctccatca cggcccacgc cgccgacgtc ggggacaacg
     9661 ccgacacggt cgaggcctcg gtcgagggct cgccgacgac catcgccttc aacgcgcgct
     9721 acctgatcga cgtgctcagt aacctcggat ccgacgaggc ggcgctcgag ctctccggtc
     9781 cgctggcgcc cggcgtcatc cgcggcatcg gcaaggacga ctacgtgcac gtcatcatgc
     9841 ccgtccgcac ggcgtcctag tcgccgatgg cgctgcgcgc cgcgccgcgc cctgtcgcga
     9901 gccggccacc catggaactg acccggctct ccctggagga cttccgcagc tacgcagcgg
     9961 ccgagctgcg gcccgacccg ggtctcaccg tcgtcgccgc cccgaacggg acgggcaaga
    10021 cgaacctgct cgaggccatc cacgtggcga tcacggggcg ctcgcaccgg gcggccgtcg
    10081 acgccgagct ggtgcgccac ggccgcccgt tcgcgcgggt ccggctcgag ctcgaccgag
    10141 ggacggccga gggcggcgcc accatcgagc tggtgcttcc cgggaccgag gcgccggcgg
    10201 ggatccgcaa gcggctgacc gtcaacgggg tgccgcgtcg ggcatcgacg gtctcggaga
    10261 ctgcccgagc cgtcctcttc cgccccgagg agatgctcct gctggtcggt tcgccgtccg
    10321 atcgccggcg attcctggac ggcatcctgg cccagcgcga ccgccgcgcg gcgcgcgacc
    10381 tggccgacct ggcccgggtc ctggcccagc gcaacgccct gctgcgcgcc atccgcgccg
    10441 aggaggcgcc actggacagc ctggccttct gggatgagca attggcccag gtcggcgctc
    10501 gcgtcatgcg ggaacggctc gcggtcgtcc gcgagctcgg cgagcgcatc ggtccattgc
    10561 acgatgccgt cgcgaccagc ggcgaacgcg gtgccgacgt gcgcctcgcg tacctcgacg
    10621 gtctcaagga ggcgtggccg gagcgcgccg atccgcgcga gcgcctgccg tcggtcgagg
    10681 agctcacggc ggcccaccgc cggcgcatca ccgacgtgcg caccaaggaa gcctggaacg
    10741 gggtcagcct ggtcggtccg cagcgcgacg atctgcgcgc cgagctgtcg ggccgggacg
    10801 tggcggcgca cgcgagccgc ggtcagcagc gcaccatcat cctggccctg aagctcgcgg
    10861 agaccgacct gctcggcgcc gatgggccgc ggccgatcgt gctgctcgac gacgtcttca
    10921 gcgagctgga tccggagcgc tcgacgcgcc tctcggagct gctggccggc cttgggcagg
    10981 tgctggtcac gaccgccgac cccggaacac tcgacgccgc ccgggcccgc ggcggggcga
    11041 tctggcggat ccgcgacggg cggctggagc ccgcgccatg agcggtcgcg aggaggcggg
    11101 agccgcggtg cagcgcgcgc tggccgagtg catgggtccg ggtgccgcgg cccagctcgc
    11161 cgtggcgcag gtccgcgtgg cgtgggccga ggtggtcgag gaggcgcgcc tgcagcgcgg
    11221 gccgctcacg agccgcgtct cgcgcgtcac cggcggaacc gcgcacgtcg aggcctccga
    11281 cccgatgctg gcccaggagc tgaccctccg ttccgaggcg ctggtgtggg ccgtgaacga
    11341 gcggatgaag gggcggccgg gggcgaccat cgtgctgcac gggctggccg tatcggtggg
    11401 tcgaggcggg gccaggccga ccctataatc gccgccagcg accacacgag cacccgggca
    11461 tcggcatgac gaaccgcttc acgaccagga tcggcctgcc gtcggcggac gagcgacccg
    11521 ccggtgccca cgacgtggtt gccttccgcg agcccaccac cggctccgag ctgcgcacca
    11581 aggggagcat cttcctcctc gcccagctga cgtcccccac gccggccctc gagcgcgccg
    11641 tcacggaggc cctcgaggcg atccagcgcg actactacta cgacctgtcg ggtggcgtgc
    11701 tggtcgccct ggcgcgtgct ctgcgggccg cgaatcgtcg gctctaccac cagcggcgcc
    11761 gcctcggtat cccgcgccgc tccggggtga gcgtcgtcgc gctcgtggtg cgcgggcgcg
    11821 aggcacatgt cgcgaagctg gggccggcca gcgcggtcgt ggtgcgcgag gcgcgcatgt
    11881 acgaggtgcc gccgccgccc gccgtcgagg acgaggaccc tcgggttcgg cgccgccgcg
    11941 tggccggcac gctgggcgag gcgctcgaga tcgagccgta cacctggcat gggcccctcg
    12001 ccgccgggga ccggctcgcg ctggtcagcc gccacttcgc ccacaccgtg ggggtcgagg
    12061 agctgaagag cgccctgtcc ggcatgcggc ccagcgcggc ggtcgagcac ctggtccagc
    12121 tgttcggcat ccgcggtggg tcgggatccg atggcctgct ggtggtcggc atcgaggagc
    12181 tgccggcgac cgccaccacc cagcacctcg agccggtgcg gccggcggag ccgttcgccg
    12241 gccttcccga ccagagcccg gtgccgctgg ccgatgccct gggtcgaggg ctgcaccgcg
    12301 ccggcgacgc tgccgaggcc gccaaggcgg ccctgggccg cggcctgctg acgctcgtct
    12361 cctggatcct ggccttcgtt ccgcgccgcc gccccgagta cccgcgcacc atcccccgca
    12421 ccgccgaacg tgaggcgggc cggcggcgac ggatcgggct ggccggcatg gtcgtcgtgg
    12481 ccgcgctgct ggccgtcggc agcacggtgg ccgcggtgcc atcggtccgg ccgaccgacg
    12541 cgatcccgcg ggccacggtg gcgcgggagg ccatcgtgga ggccgtcgac ctgctcgacc
    12601 tggtcgggca gcgcgtcgac ggcgccgacc tggtggaccg cgacccggag cgcgcgacgg
    12661 atctgctggc cgatgcgcgg gccgcgctcg accgggccgc tggcgcgggc gttcgtcacg
    12721 accagctgcg gccgctgcgc tcgcgcgtcg acgaccgcct ggacgcgatc taccgcgtca
    12781 cgcgcatcgc ggcgccggat acggtggcgg cgctggggga ggactacgac gacctcgaac
    12841 ccctccacat ggtggctgcc agcgacggct cgctgtgggt gctggacggc ggccgcggcc
    12901 gcgtcatccg ggtcgatccc gtcgacggca gcagcaccgt ggtgtaccgc gccggccagg
    12961 cgctcgagtc gggcgacgta cccggcgatc cgtggctcat ggccacggcg gcgaccgacg
    13021 tggtggtcat cgaccgcgcc cggcaggcct ggcgcatgga ccttaccgag cgcatcccgc
    13081 gcccgatggt gctcgcgggg gtcgaggcgc tcggctccga caccaccctg ctcggcgcgc
    13141 tccagcaccg cccgccgctc gagatcttca acctctacgc ggtggacgcg accaccggcc
    13201 aggtcctgcg ctggacgcca cccgccgtca tcccggtcaa ctatcccgat ccacccgagc
    13261 cgttcctgac cgatgagccg gatctcgacc cgcgcgacgc gcgtgacctg cgcgtcgacg
    13321 tcaacgcctg gctcctccac gccggcaccg tcacccgcgt cgacttcggc tcgccgcgtg
    13381 accaggtcga gtactcgctc gacccgcccc ccgacgatgc ggtgcggccg acgctcgact
    13441 accggctgct ggacggcgcc accgtcggcg atcgcgagct gctgtacgtc tacgacgcgg
    13501 cgagctcgcg gatcatcgcc ttccagcgcg ccgacggcgc cttcgtgcgc cagtggctgg
    13561 cgcccgcgga cgggccggcc gcgggctccc tcgacgaggt catcgggctc tcggtgacct
    13621 cggtggcgga cggtccgccg accgcgtacc tgctcacgcc cgacggggtc gttcggctcg
    13681 tcctcgagta gcctcggcta gaatcggccc atggcggacc agcccttcgg gcgcgtgcgg
    13741 ctcgtggaga tggtggtgga gagcgtccgc gtgcacatgc tgtcgagcca gcacgtcgtc
    13801 atcctcaagg agtccgagcg cgatcgctac cttccgatct ggatcggctc cggcgaggcc
    13861 aacgccatcg ccatgcgcct ccaggggctc agcgccgagc gaccgctgac ccacgacctg
    13921 ctggtgagcg tcatcggcgc cctcaactcg agcgtcagcc gggtggtggt cacgcatgtc
    13981 acggacggca ccttccacgc ccgcctgtac ctggagacgg ccaacggcga cgaggccgag
    14041 gtcgactcgc gcacctcgga tgcgatcgcc gtggcggtgc gcaccgggtc gaccatctac
    14101 gtcgacgagc gggtgctcga cgaggctggc gtggagcccg attcgacgga ggagacgacg
    14161 ccggaggagg acgagcggct ggcggtgttc cgcgagttcg tgaactcgct ggacgtcgac
    14221 ctcccggagc gcggccccga ggccggccct ccccccgagg agtcctaggc cgagccgccg
    14281 gcctcgcggg cgcgaaccgg accgagggtc tccgagcgtc gggtccacag cgcccagcgc
    14341 agccgtccga tgtcgcggaa ggtgcgcagg atcttgcgga agctggcccc ggtgttcgtg
    14401 ccggccgcgc gggggtagtg ggtgacgccg acctgcgcca tccgctcgcc gcgcgccttg
    14461 agcttgatca gcagctcggc cgagaggaag ggcgactcgg cggtcaggtt gaggccgtcg
    14521 aacacctcgc gccggaagac cttcatggcg cagtcgatgt cgcggacccg cagcccgaag
    14581 gccagcgaga cgatggcgtt gtaggtcttg gcgatgacga tgcggtgcgg cgggtcgcga
    14641 cgcttgatgc gccagccgat gaccgcgtcg aaccggccgc gcttcggatc gtcgagccgc
    14701 tccagcagcc gcgagaactc gcgcaggtcg aactgcaggt cgccgtcgct gaagccgatg
    14761 agctcgccgc gggcattgtc gaagccggcc cgcagggcgc cgccgtagcc gcggttcggc
    14821 tggtgatgga cacggacgcg cgggtcggcg gcggccagcg catcggccag ctcgggggtc
    14881 cggtcggtcg agccgtcgtc caccaccagc acctcgatcg agtcatccac cagcggtccg
    14941 acctcgtcga gcgcgcgccg cacggtctcc gcgacgttct cctcctcgtt gtaggcgggg
    15001 aagaagaacg tcaggcgcgg acgctcggga tcggtcatcg acggcggccc tcgatcagca
    15061 gcaggtccgt gtagccgcgg taggtgctca cgttgcggta ctcccagtca cgatccagcc
    15121 tccgccactc ccagtcgggc ggcaccacgt agacggggcg ttcgccgtag aaggaggcga
    15181 tcgcgttgcg catcgtacgg tacccgtcgt cgaggatgtt gcgctcgtcg atgatcgtga
    15241 catccgggcg cgcgccgtcg atccaccggt ggtaccacag cggtgtggag tagctccacc
    15301 agctgatgat cacggcgttc ggcggcagct gctcgtacac cgatgcgacc cactggtcgg
    15361 cctcacggtt cgtgctctgg tcgtgcgagg cgtagccggt gacgaggccg acggccggca
    15421 gcagcatcga ggccgcgagc acggtgccac ccaccaccgc cgtcacgcgg cggcggacgc
    15481 gccgcggcat cgcgttccgc aggcccatct cgccgacggc gcgggcaccg gcggtcgcga
    15541 ttgcgcccac cgccacggcg agcagcggtg cggtcgccac caccgtggtc atgtagtacc
    15601 ggtcgatgtc gccgtcgcgg aagttcatcg agtacaggac gttggccacg acgaccagcc
    15661 ccaggaaggc cagcaccccc ggccgtcgca cggcgaggac ggccgcgcca ccggcgacca
    15721 gcagccagcc ggggccgatg aactgggccg acagcacgcg ctcggcgtcg acccacttgc
    15781 tgctcagctc agccagcggg tcgcggaagt cgtgaaagag gctcttgaac tgctcggcga
    15841 acaccaggta gcggaagcgc tcccacgtgt ccggatgcgc gtagaacagc ggcggagcgg
    15901 gatcgatgtt ggcgcggatc cagatgaagg cgtacgagcc gatgccgagc gccaggaccg
    15961 cggcgcatgc cagcaccagc cgccatcggc gccagaggtg accatggacg accagcagcc
    16021 acgcggcgat gccgaaggcg gtgaggccga ccagcgggtg cacgcccatg ccgaggccga
    16081 acgcgaaggc cgcaccgatc agccagccgc ccgcgcgtcg ggccccggcc cgctccgcgg
    16141 cgccccacgc gaacagcagc cacaccacca gcgcgacgaa gaagaccgtg atggcgtgga
    16201 cgtcggcgcg cgagctgttc tcccacggct cggacgcgaa cgcgaaggcg gcgccgccga
    16261 tgccggccgc gaccgccgcg acggaccggc tcggctcccg caccaggtgc ccggcgatca
    16321 acgtcaccag cccggcagcc agcgcgacgc acaccgccga gagcaggttc atgcgccacg
    16381 ccacctcgcc ga
//
LOCUS       DASTYP010000017        15964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_547560, whole genome shotgun sequence.
ACCESSION   DASTYP010000017 DASTYP010000000
VERSION     DASTYP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547560"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFV59_00840"
     CDS             <1..1063
                     /locus_tag="VFV59_00840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5324412.1"
                     /translation="VNASTDREVTVFSAKVPAARAEAALAAVSELALRPMLRRRDLAA
                     EKPVIADEIRMYVDSPSDHVFTLFDELMFGRHPLGREIAGSIGSVRRATHDGVVGHWR
                     AAYRPGAMVLSAAGAIDHDELSATAEGWRAEGPAVGGHRRPTRPPIAIAAPEPAPAGA
                     LRVSHRRLSQANVCLGMPAVARDHPDRWALDLLGAILGDGMGSRLFIELRERRSLVYD
                     VSTFSSLYSDTGVFGVHAGFDPEDAAKVVGAILDQLDRVVTDAPGEEELARARAYTRG
                     RLELRMEDSAAVAGWLGMGETLLPRILTVDEVIERLEAVTREDLLRVARTYLAPDQVR
                     LAALGPFRSRRPFERAMRR"
     gene            1060..1857
                     /locus_tag="VFV59_00845"
     CDS             1060..1857
                     /locus_tag="VFV59_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324413.1"
                     /translation="MSDEAVDDVHVVDLVTDELTLARSQLASGLPGVAEGTLRRRLAR
                     LEAEGAGIGDEADAARAVLAEALWRQHRPRAARAALDQIRPSSPQRRLPMTMLIEAES
                     LAVAGELDRATGTMERVVAAVGIDRADELRAGVPGRLSWPLPADLRPQPPAAGRPPWT
                     PEPSANIDGESAGRPDDERATEARRRLEEARVAYVAGDLERGDTELGIAVRLDPALSA
                     DGVVILEPTLGGQPSAERLLRYGDLLRAAGRQVEAERAYDRAAESRG"
     gene            1922..2383
                     /gene="ndk"
                     /locus_tag="VFV59_00850"
     CDS             1922..2383
                     /gene="ndk"
                     /locus_tag="VFV59_00850"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5324414.1"
                     /translation="MQRTLVLAKPDAVQRGLVGEIIGRFERKGLKLVGLRLLSVPREM
                     AEEHYAVHAGKHFYAGLVEFITSGPVAAFALEGPDAIAVVRKMVGKTMPNEAEPGTIR
                     GDLGVSGLRNLIHASDAPETAEAELALWFGESALVDYTRDVDTWIVAEEPA"
     gene            complement(2509..3219)
                     /gene="lepB"
                     /locus_tag="VFV59_00855"
     CDS             complement(2509..3219)
                     /gene="lepB"
                     /locus_tag="VFV59_00855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5324415.1"
                     /translation="MTYVPDEADAPGAPVPHERAGSRRALGCAFEIVETLVLTLLIYL
                     VIHNFVAQPFEVQQTSMFPTIVNGEYILIDKLTTRFDGYHYGDIIVFEPPGDSGLDTD
                     GVPFIKRIIGMPGDTVSLEHGRVFITRPGEPAVRIEEPYVVTQADGTTAATLPRDAQG
                     TTTWTVGQNEYFVMGDNRPSSQDSRAFGPISKDLILGRAWLRYFPLDRITLFERPSYP
                     ALEGGRTGLRLVSLPAAA"
     gene            3286..3927
                     /locus_tag="VFV59_00860"
     CDS             3286..3927
                     /locus_tag="VFV59_00860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5324416.1"
                     /translation="MGTLILARHSITAASSEGRNLGQRTDPPLADAGVALAAELGRTI
                     VGELAELPHDELRLLSSPARRCRQTAAAIADALALVEEAIEVQAGLLEIDYGEWDGLT
                     PEECHARDPELRTAWEADPYATRCPNGESGADVRARADDVLVPLERWLAEDRARCAVV
                     VAHNHVNRVHLCALFGWPMAEYRDRIVQDPAGYSLVTFGGPIPVIRRMNAAPA"
     gene            4003..5418
                     /gene="rpoN"
                     /locus_tag="VFV59_00865"
     CDS             4003..5418
                     /gene="rpoN"
                     /locus_tag="VFV59_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU5324417.1"
                     /translation="MSLELNQQLQPVMQVKVTPKQIAANYILQMSSVELQEAIAQELD
                     ENPALEMQELPTCPICGNPVVGNYCTECMPRKSEGDGDETRLSADDLAGDGQTRLRDE
                     EDEFDPIARAEADFTLEDHLTWNLHALLPSRLHHVADHVIGALDSNGFLTETDAEIAS
                     ETGVSVADVDEVLGAMKGLEPIGIGSRSITESLLAQVEYLREQNEIEVPPFAEEVIAQ
                     HLSDLGERRFREVASSIGCTQRDVIAVWEFVKANLNPYPTAAFTAAVSGDSSRPIIRP
                     DVIIRDVDGELGVEVVESRRFSLRVAPIYSSLSSKLRSAETTEADKEHVRQYVGRAKF
                     FIDNINRRRATIQRIAEALVDRQRDYLLNGVQHLVPLTRAEVGELIGMHESTVSRATA
                     EKYVMIPSGEVVPFSHFFTASLGIKDQIKRMIEAEDPKHPLSDQEIADALGEEGIALA
                     RRTVAKYRDELQILPARLRHR"
     gene            5439..7481
                     /locus_tag="VFV59_00870"
     CDS             5439..7481
                     /locus_tag="VFV59_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5324418.1"
                     /translation="MRAVVLRSLVVIGIGAVLLAGVLYVASTVDARAPEVIGVRLTQP
                     APDQQAVAQITTSIEVEFNEPVEPDSAAQALRIEPPVPGSVSWSGSTMIFTPAEPLRP
                     ETDYVLSLDAGVRDLSGNEMAQLPDPFAFATVGRPTVSASEPADGDRDVALDATIVLT
                     FSSLMDTASVEAALRLQPAFPHELRWSGDQLEIVPTQPLEAGREYRVRIREGAADVSG
                     ATLARGVSIGFRTIEPGLRAATVVPSDGIAGIAPTSPIAVIFDKPINPGSVERALLTI
                     TPDVAGSLEVVAVPGEERDDGAGRILRFTPSGPLPSTTTFEVTLAAAITGVDGEGLGR
                     EISWSFTTGAPVGTLSNQIAFLSDRAGVTNVWVMNPDGTGQRQLSAELTPVTDYAVAP
                     DGSTLAVTDGRRIVVLRPDGSDRRVLTPDGAIDVDPAYSPNGSELVFARLAAPSDRPT
                     GLWRWEIGAGDPAPIELPVEDGPTTSPTPSPTPSPTTSGGADVTPTGTLRAPRFAPDG
                     RAIAFVDVSGSVGILELEDETLSVAAVAAAAPPVWLPDSSGVLLSPGRVERTETAPVP
                     PLDGAAEGPLEVLRRGRSGTTPADVGGVRVLALAADGRLAVAGADRRLRVADAPDEPA
                     RATSLGAEAVAGVAWAPSEASVLAVTLEAGNLVLFDLEDGSTRQLAQSGASPRWLP"
     gene            7606..9834
                     /locus_tag="VFV59_00875"
     CDS             7606..9834
                     /locus_tag="VFV59_00875"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEU5324419.1"
                     /translation="MELITWVIPIAGLAAIAFALYLAWDVLRADTGTPAMQDIAGTIY
                     EGAVAFIRRQYQTIAMLALGGAVVIGLVIGLVEGREVADTSVFGFDLGWRTALAFLVG
                     AACSMASGIIGMFISVKSNLRTAAAAQHSLVRAVQVAMRGGAVSGFLVVALSLLGVYG
                     IFAAFGGFSNPAEAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIP
                     EDDPRNAGVVADLVGDNVGDCAGRGADLFESTAAENIGAMILGVAVYTIASASGWPNP
                     EAWIFFPLVVRAFGLLATIVAMFFVRGSETEDPMNMLNRGYWVTTILSVAALALTTFV
                     MMNTGGETPANGIPPWVWFFGAGVVGLATSVAFVYITQYYTAGAWRPVKEIATASKTG
                     PATNIISGTAVGFETTAVTAITIGIALFVSHWLGEQAGLIQTLDDGTTRNVGGIFGTA
                     VATMGMLMTTAYILAMDTFGPITDNAGGIAEFSKAEGGAREITDRLDAVGNTTKALTK
                     GYAIASASLAAFLLFSAYIDKVNLILTRQIESGIREAGTLLEAVDLADVNVFIAALIG
                     AMLVYFFSSLAIRAVGTAAQAIIVEVRRQFREMPGIMDYTQRPDYARVVDITTRAALR
                     QMILPGTVAVATPIIVGLLLGHEAVAGLLMVGTIAGVLLATVLNNGGGAWDNAKKFIE
                     AGHLTDDAGNVLGKGSDAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATITLVLAPLF
                     IR"
     gene            9881..10726
                     /locus_tag="VFV59_00880"
     CDS             9881..10726
                     /locus_tag="VFV59_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU5324420.1"
                     /translation="MDLIIQAALVGLLQGLTEFIPISSSAHLELAPWIAGWGSDGLIG
                     SLAFDVFLHLGTFVALVAYFWRDWVRYIAAWFASVRERRIGEDSDRRIAWLLLAATIP
                     AAIIGFALEDFITEAFHGDSDGARLAIAGFLVIGATALWLADRYGRRQRPMENLSTGS
                     ALVIGFSQALALFPGISRSGATITAGLALGLTREAAARFSFLLATPITFGAGVYGSRH
                     LLTEAHTSSEWVAIGVGFAVAALSGMLAIGFLLAWLRRRSVAIFSIYRIAFAALVVVL
                     VVLGR"
     gene            11021..11482
                     /gene="greA"
                     /locus_tag="VFV59_00885"
     CDS             11021..11482
                     /gene="greA"
                     /locus_tag="VFV59_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU5324421.1"
                     /translation="MNTKPVYLTAEGLEKLKAELNELVTLERPRVAARIHEAKLDGDL
                     SENAEYEDAKQEQSFLEGRIATLELQLRNAAVIEKTNGDSVGIGSKVVIKGSDGEETF
                     TIVGSAEAAPRDGRISNESPVGVALMGRKKGDKVVVNAPSGPIEYTVVRVG"
     gene            11491..13098
                     /gene="lysS"
                     /locus_tag="VFV59_00890"
     CDS             11491..13098
                     /gene="lysS"
                     /locus_tag="VFV59_00890"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU5324422.1"
                     /translation="MFWADELAASATGRQVVNDSKTPSGTVHVGSLRGPVVVDAIARA
                     LRDAGREVELRYGVDDLDPMDAQALLTPDAIDRYMGVPLAHVPDPAGDCHASYARHFA
                     GVFVDTFDGLGIRPDTYYWMSEVYASGAMDRYIRAALDAAPTIREIYRRVAKVNHPDE
                     WHPLFVICESCGRIGTTIVTGWDTERGVVRYECRTDLVRWATGCGHAGEVSPFGGRAK
                     LPYNVDWAAKWDLFGVTIEPAGKDLSTAGGSRDRSDAIAREVFGNEPPRNLPYEFLNI
                     GGRKMSTSKGVGAAATEIADVIPPEQLRLLFLRPRPNTAIEFDPYGTDAIPRLFDESD
                     RLAAATAGREVRGELPPDHERVFALTQVDADADVAALAAAYRPAFSHLALLEQIPGVD
                     VEERVAVEKGGPLTSHERDLLEERRRAARAWLEAYAPERARLAVQRDALPAAAAELDD
                     AQRAYLAALADALAGVEWSGEAVQAAIFATATANDLPAGRAFAALYAAFLGRTSGPRA
                     GWLLAALERDFVIGRIRDAASAARVAS"
     gene            13095..14426
                     /gene="serS"
                     /locus_tag="VFV59_00895"
     CDS             13095..14426
                     /gene="serS"
                     /locus_tag="VFV59_00895"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003392668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU5324423.1"
                     /translation="MSVGLQRLRDDADALRRAATDKGEDPALVDLAVALDERRRVLLA
                     EGDALKAERNASSKRIGEAIRGGADPGGPDVADLKVRSTAVGERIRAVDEELARVEAE
                     LDDAMLRIPNPADPDVPIGGEEANLVARSWGEVQARVDDSDPEYGPWERRPHWELAES
                     LGMLDNARGAKVAGSGFPVYLGAGSNLQRALINWFLDVHTREHGMTEVWPPVLVNTDS
                     ARGTGQIPDKEDQMYVVTRDDLYLIPTAEVPVTNLHRDEILDGAMLPLRYAAYSASFR
                     REAGAAGKDTRGILRVHQFDKVEMVSFTRPEESAGELEWLTERAEVLLQRLGLTYRVL
                     LMATREMGFTQARKYDLEVWAPGVERWLEVSSVSNFRDFQARRMGIRFRPDAGARPEL
                     VHTLNGSGLALPRTVAAILETYQRPDGSVEIPEVLRPYFGGQELIGPGLSA"
     gene            14477..14878
                     /locus_tag="VFV59_00900"
     CDS             14477..14878
                     /locus_tag="VFV59_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HEU5324424.1"
                     /translation="MSIPALLATFGVMAVLDGIWLTLVARNFYRDNIGHLLGDATNWP
                     PAILFYVIYTIGAWFFATQPGVDDGSAITAALRGAAFGFVAYATYDLTNHATLRGWPS
                     LVTVVDMAWGTILTATVAGVAAWITLTFLAS"
     gene            complement(14853..15809)
                     /locus_tag="VFV59_00905"
     CDS             complement(14853..15809)
                     /locus_tag="VFV59_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5324425.1"
                     /translation="MTDTTTATILDQPRVPAASIDWLAIGAALLTVLLWASAFVGIRA
                     VADDLSPGSFALGRLLVGALALGVLVALRRPALPGRRRDWLLIAAAGVVWFGTYFVAL
                     NAGEGLVDAGTAAMLVNIGPILIAAFAGIFLHEGFPPRLVAGSAVAFAGTLVIGVASG
                     TASGEASAPIGVLLCLVAALAYALGVTLQKPALKRVPALTVTWIGCTVGAIATLPFGP
                     LLVQELGTAPADAIAWIVYLGIFPTAIAFTTWAFALNRTTAGRLGSTTYLVPPVAIVM
                     AWLLLGEVPPALAFAGGALCIGGVIVARSGGRIGLRRRGTSA"
     gene            15877..15964
                     /locus_tag="VFV59_00910"
     tRNA            15877..15964
                     /locus_tag="VFV59_00910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15911..15913,aa:Ser,seq:tga)
BASE COUNT         2281 a         5813 c         5608 g         2262 t
ORIGIN      
        1 ggtcaacgcc agcaccgacc gcgaggtgac ggtcttctcg gccaaggtcc cggccgccag
       61 ggccgaggcc gcgctggccg ccgtcagcga gctggccctg cgcccgatgc tgcgccggcg
      121 ggacctggcc gccgagaagc cggtcatcgc ggacgagatc cgcatgtacg tcgactcgcc
      181 gtcggaccac gttttcaccc tgttcgacga gctgatgttc ggccgccatc cgctgggccg
      241 cgagatcgcc gggagcatcg gctccgtgcg ccgcgcgacg cacgacgggg tggtcggcca
      301 ctggcgcgcc gcgtatcgcc cgggcgccat ggtcctctcg gccgcggggg ccatcgacca
      361 cgatgagctg tcggccaccg cggaggggtg gcgggccgaa ggtcccgccg tcggcggcca
      421 ccgccgaccg actcgtccgc cgatcgcgat cgcggccccc gagccggctc cggcgggcgc
      481 gctccgcgtg tcgcatcggc gcctgagcca ggcgaacgtg tgcctgggca tgcccgccgt
      541 cgctcgcgac caccccgacc gttgggccct cgacctgctg ggggcgatcc tgggcgacgg
      601 gatggggagt cgactcttca tcgagctgcg cgagcgccgc tcgctggtgt acgacgtgtc
      661 gaccttcagc tccctctact ccgatacggg tgtgttcggg gtccatgccg gcttcgaccc
      721 cgaggacgcg gcgaaggtcg tcggcgccat cctcgaccag ctcgatcgcg tcgtgaccga
      781 tgcaccgggg gaggaggagc tcgcccgagc ccgcgcctac acgcgcgggc ggctggagct
      841 gcgcatggag gacagcgcgg ccgtcgccgg ctggctgggg atgggggaga cgctgctgcc
      901 gcgcatcctg accgtcgacg aggtgatcga gcggctcgag gccgtgaccc gcgaggacct
      961 gctccgcgtc gcccggacgt acctggcgcc ggaccaggtc cgcctcgcgg cgctgggacc
     1021 cttccgcagc cgccgcccgt tcgaacgagc catgcgccga tgagcgacga ggccgtcgac
     1081 gacgtccacg tcgtcgacct ggtcaccgac gagctgaccc tcgcccgctc gcagctggcg
     1141 tcggggctac ccggggtggc ggaggggacg ctgcgacgcc ggctggcgcg gctcgaggcg
     1201 gagggtgccg gcatcggcga cgaggccgac gccgcgcggg cggtgctggc ggaagcgcta
     1261 tggcggcagc atcggccgcg ggcagcccgt gccgcactcg accagatccg accgagcagc
     1321 ccgcagcgac ggctgccgat gacgatgctg atcgaggccg agtcgctggc cgtcgccggg
     1381 gagctggacc gcgccacggg caccatggag cgcgtcgtgg cggcggtcgg catcgaccgc
     1441 gccgacgagc tgcgcgccgg agtgccgggt cggctctcgt ggcccctccc ggccgacctt
     1501 cgaccgcagc ctcctgctgc cggccggccg ccatggaccc ccgagccgtc cgcgaatatc
     1561 gacggcgagt ccgcggggcg tcccgacgac gagcgcgcca ccgaggcgcg gcgacggctg
     1621 gaggaggcgc gcgtggcgta cgtggccggc gacctcgagc gcggcgacac cgagctgggg
     1681 atcgccgttc gcctcgatcc ggcgctgtcg gccgacggcg tcgtgatcct ggagccgacc
     1741 ctcggcgggc agccgtccgc ggagcgtctg ctacgctacg gcgacctcct ccgcgctgcc
     1801 gggcgccagg tcgaggccga acgggcctac gatcgcgccg ccgagtcgcg gggctgagca
     1861 ccccgacgca tcccatcgac ctgaccggtc gccccgaatt cgacaggagc ctcacgtccc
     1921 catgcagcgc acgctcgtcc tcgccaagcc cgacgcggtc cagcgcgggc tcgttggcga
     1981 gatcatcggc cgcttcgagc gcaagggcct caagctcgtc ggcctgcgcc tcctctccgt
     2041 cccgcgcgag atggccgagg agcactacgc cgtccacgcc ggcaagcact tctacgccgg
     2101 cctggtcgag ttcatcacct ccggtccggt ggccgccttc gcgctcgagg ggcccgatgc
     2161 catcgccgtg gtccgcaaga tggttggcaa gacgatgccc aacgaggcgg agcccggcac
     2221 catccgcggg gacctgggcg tcagcggcct tcggaacctg atccacgcct cggacgcgcc
     2281 cgagacggcc gaggcggagc tggcgctctg gttcggcgag agcgcgctgg tggactacac
     2341 ccgggacgtc gacacctgga tcgtggccga ggagccggcc tgagcgccgt ccgctcctgc
     2401 gatggctgac gcgccccacg ggtcacgcga cggccgaagg catcccgaag ccccgctcaa
     2461 cccaccattg acccgtccca cgggtcgctc gaggacggcg gcccttcgtc aggcagccgc
     2521 agggagcgag acgagtcgca ggccggtccg accgccctcg agcgcggggt aggacggccg
     2581 ctcgaagagc gtgatgcggt ccagcgggaa gtagcggagc caggcccgtc ccaggatgag
     2641 gtccttgctg atcgggccga aggcgcgcga gtcctggctc gaggggcggt tgtcgcccat
     2701 cacgaagtac tcgttctgac ccacggtcca cgtcgtggtc ccctgcgcgt cacgcggcag
     2761 cgtggcggcg gtcgtgccgt cggcctgggt gaccacgtac ggctcctcga tccgcaccgc
     2821 cggctcaccc ggccgggtga tgaagacccg gccgtgttcg agcgagaccg tgtcgccggg
     2881 catgccgatg atgcgcttga tgaacggcac accatcggta tccagcccgg agtcaccggg
     2941 cggctcgaac acgatgatgt cgccgtagtg gtagccatcg aaccgcgtcg tcagcttgtc
     3001 gatcaggatg tactcgccgt tgacgatcgt cgggaacatc gaggtctgct gcacctcgaa
     3061 cggctgggcc acgaagttgt ggatgaccag gtagatcagc agggtcagga cgagcgtctc
     3121 gacgatctcg aaggcgcagc ccagcgcgcg acgcgaaccg gcgcgctcgt gaggcacggg
     3181 cgcgccggga gcatcggctt cgtcggggac gtaggtcatc aggcggcagg tgcgggcaac
     3241 ggccgcgaca ggatagcgca gacgcgcggc tagacttgcg ggtccatggg cacgctcatc
     3301 ctggctcgac actccatcac cgccgcctcc tccgaggggc gcaacctcgg gcagcggacg
     3361 gatccgccac tggccgatgc gggcgttgcc ctcgccgcgg agctggggcg cacgatcgtg
     3421 ggagagctgg ccgagctccc gcacgacgag ctgcggcttc tcagcagccc ggcccgacgt
     3481 tgccggcaga ccgccgccgc catcgccgac gcgctcgcct tggtcgagga ggcgatcgag
     3541 gtgcaggccg ggctcctgga gatcgactac ggcgagtggg acgggctgac gccggaggag
     3601 tgccacgccc gcgacccgga gctgcgcacc gcctgggagg ccgacccgta cgcgacgcgc
     3661 tgtccgaacg gcgagagcgg tgccgacgtg cgcgcccgcg ccgatgacgt gctggtcccg
     3721 ctcgagcggt ggctggccga ggatcgggcg cgctgcgccg tggtggtggc gcacaaccac
     3781 gtcaaccgcg tgcacctctg cgcgctgttc ggatggccca tggcggagta tcgggaccga
     3841 atcgtgcaag accctgccgg gtacagcctc gtgacgttcg gcggaccgat cccggtcatc
     3901 cgccgcatga acgcggcgcc cgcctgacgg ccgatccttc gatcggccgc cttcggcgta
     3961 gaatccgagt cccacccgat caccaccaac gcgcgaggtt ccatgtcgct cgagctcaac
     4021 cagcagctgc aaccggtcat gcaggtcaag gtcacgccga agcagattgc ggcgaactac
     4081 atcctgcaga tgagctcggt ggagctgcag gaggcgatcg cccaggagct ggacgagaac
     4141 cccgcgctcg agatgcagga gctgccgacg tgcccgatct gcggcaaccc ggtggtcggc
     4201 aattactgca ccgagtgcat gccccgcaag agcgagggtg acggcgacga aacgcgcctc
     4261 agtgcggacg acctggccgg cgacggccag acccgcctcc gtgacgagga ggacgagttc
     4321 gatccgatcg cccgggcgga ggcggacttc acgctcgagg accacctcac ctggaacctg
     4381 cacgccctgc tgccgagtcg gctgcaccac gtggccgacc acgtcatcgg cgcgctcgac
     4441 tcgaacggct tcctgaccga gaccgacgcg gagatcgcgt cggagaccgg cgtctcggtc
     4501 gccgacgtcg atgaggtgct gggggccatg aagggcctgg agccgatcgg catcggctca
     4561 cgctcgatca ccgagtcgct cctggcgcag gtcgagtacc tccgcgagca gaacgagatc
     4621 gaggtgccgc cgttcgccga ggaggtgatc gctcagcacc tgtccgacct gggggagcga
     4681 cgcttccgcg aggtcgcctc gtccatcggc tgcacgcagc gcgacgtcat cgcggtctgg
     4741 gagttcgtga aggccaacct caacccctac ccgacggcgg ccttcaccgc cgccgtcagc
     4801 ggtgactcgt cgcggccgat catccgcccc gacgtgatca tccgcgacgt cgacggcgag
     4861 ctgggggtcg aggtggtcga gtcgcggcgc ttcagcttgc gcgtggcccc gatctactcg
     4921 agcctatcgt ccaagctgcg cagcgccgag acgaccgagg ccgacaagga gcacgtgcgc
     4981 cagtacgtgg ggcgcgcgaa gttcttcatc gacaacatca accggcgtcg cgcgaccatc
     5041 cagcgcatcg ccgaggccct cgtcgaccgg cagcgcgact acctgctcaa cggcgtccag
     5101 cacctcgtgc cgctgacgcg tgccgaggtg ggcgagctga tcggcatgca cgagtcgacc
     5161 gtcagccgcg ccaccgccga gaagtacgtg atgatcccgt ccggcgaggt ggtcccgttc
     5221 agccacttct tcaccgcctc cctcggcatc aaggaccaga tcaagcggat gatcgaggcg
     5281 gaggacccga agcacccgtt gtccgaccag gagatcgccg acgcgttggg ggaggagggg
     5341 atcgcgctcg cgcgccggac cgtcgccaag taccgcgacg agctccagat cctgccggct
     5401 cgcctcaggc accgctgacg cctcggtcga cgcgtccgat gcgcgccgtc gtcctgcgct
     5461 ccctcgtcgt catcggcatc ggtgccgtgc tgctcgccgg cgtcctgtac gtggcgagca
     5521 ccgtcgacgc gcgggcgccg gaggtgatcg gcgtgcggct cacccagccg gcacccgacc
     5581 agcaggccgt cgcacagatc acgaccagca tcgaggtcga gttcaacgag ccggtcgagc
     5641 cggacagcgc ggcgcaggcc ctgcggatcg agccgcccgt ccccggcagc gtgagctgga
     5701 gcggcagcac catgatcttc accccggccg agccgctgcg cccggagacc gattacgtcc
     5761 tctcgctcga cgccggcgtg cgcgacctgt ccggcaacga gatggcgcag ctgcccgacc
     5821 cgttcgcgtt cgcgaccgtc gggcgcccga ccgtctccgc atcggagccg gccgacggtg
     5881 accgcgacgt ggcgctggac gcgacgatcg tgctgacctt ctcgagcctg atggacaccg
     5941 cctcggtcga ggccgcgctc cgcctgcagc ccgccttccc gcacgagctg cgctggagcg
     6001 gcgatcagct ggagatcgtg cccacgcagc cgctcgaggc ggggcgcgag taccgcgtcc
     6061 ggatccggga gggcgcggcc gacgtgtccg gcgccacgct ggcccgcggc gtgtcgatcg
     6121 gcttccggac gatcgagccg ggcctgcggg cggcgaccgt cgtgccgagc gacggcatcg
     6181 ccggcatcgc gccgacgagt ccgatcgccg tcatcttcga caagcccatc aatcccggtt
     6241 cggtcgagcg cgcgctcctg accatcaccc cggacgttgc cggcagcctg gaggtcgtgg
     6301 cggtgcccgg cgaggaacgc gacgacggcg cggggcggat ccttcgcttc acgccttccg
     6361 gcccgctgcc cagcaccacg accttcgagg tgacgctggc ggccgcgatc accggggtcg
     6421 atggtgaggg cctcggccgt gagatcagct ggagcttcac gaccggcgcg ccggtcggga
     6481 cgctgtcgaa ccagatcgcc ttcctgagcg accgcgccgg cgtcaccaac gtgtgggtga
     6541 tgaaccccga cggcaccggc cagcgccagc tctccgcgga gctgacgccg gtcaccgact
     6601 acgccgtcgc gcccgacggc agcacgctgg cggtcacgga tggccgccgg atcgtggtcc
     6661 tgcgtccgga cgggtcggat cggcgcgtgc tcacgccgga cggcgccatc gacgtcgatc
     6721 cggcctacag ccccaacggg tcggagctcg tcttcgcgcg gctggcggcg ccgtccgacc
     6781 gacccaccgg cctgtggcgc tgggagatcg gcgcgggcga tccggcgccc atcgagctcc
     6841 ccgtggagga cggccccacc accagtccca cgccgagtcc cacgccgagc cccaccacct
     6901 cgggcggggc cgacgtgacg cccaccggaa ccttgcgcgc tccgcgcttc gcgccggacg
     6961 gtcgggcgat cgccttcgtc gacgtctcgg gctccgtcgg catcctcgag ctcgaggacg
     7021 agacgctgtc ggtcgccgcg gtggccgcgg ccgctccgcc ggtgtggctg cccgactcgt
     7081 cgggcgtcct gctctcgccg ggccgtgtcg agcgcacgga gaccgcgccc gttccgccgc
     7141 tcgacggcgc cgccgaggga ccgctcgagg tcctgcgccg cggccgtagc ggcaccacgc
     7201 cggccgacgt cgggggcgtc cgcgtcctcg ccctggccgc agatgggcgg ctggcggtcg
     7261 caggcgccga tcgcaggctg cgcgtcgcgg atgcgcccga cgaaccggcg cgtgccacgt
     7321 cgctcggcgc ggaggcggtc gccggcgtcg cgtgggcgcc gagcgaggca tcggtcctgg
     7381 cggtcacgct ggaggccggc aacctcgtgc tcttcgacct cgaggacggg tcgacgaggc
     7441 agctcgcgca gtcgggcgcg tcgccacgct ggctgcccta gcgcgtgcta gaatccgccg
     7501 gccaccgctg gcgggtcacc tcgtgtccga ccgccggcat ttcgtgcgtc aacccccggc
     7561 cgccatcgcg cctgtctgtg ggtgccggcc gcattcggag gactcatgga gctcatcacc
     7621 tgggtgatcc cgatcgccgg tctggcggcg atcgccttcg ccctctacct cgcctgggac
     7681 gtcctgcgcg ccgatacggg cacgcccgcg atgcaggaca tcgcggggac gatctacgag
     7741 ggtgccgtcg cctttatccg gcggcagtac cagaccatcg cgatgctcgc gctcggtggc
     7801 gccgtcgtca tcggcctcgt catcggcctc gtcgaaggcc gcgaggtggc cgatacgagc
     7861 gtcttcggct tcgacctcgg ctggcgcacg gcgctcgcct tcctcgtggg tgcggcgtgc
     7921 tcgatggcct ccgggatcat cggcatgttc atcagcgtga agtcgaacct gcggaccgcc
     7981 gccgcagcac agcacagcct ggtgcgtgcg gtgcaggtcg ccatgcgcgg tggcgccgtc
     8041 tcgggcttcc tggtggtagc gctcagcctc ctcggtgtct acggcatctt cgccgccttc
     8101 ggcggcttct cgaacccggc cgaggcgccg ttcctcatcg tcggcttcgg cttcggcgcc
     8161 agcttcgtgg ccctcttcgc gcagctcggc ggtgggatct acaccaaggc cgccgacgtg
     8221 ggtgcggacc tggtgggcaa ggtcgaggcg ggcatccccg aggacgaccc gcgcaatgcg
     8281 ggcgtggtgg ccgacctggt cggcgacaac gtcggggact gcgccggccg tggtgccgac
     8341 ctgttcgagt ccacggccgc cgagaacatc ggcgccatga tcctgggcgt cgcggtctac
     8401 acgattgcga gcgcctcggg ctggccgaac ccggaggcct ggatcttctt cccgctcgtc
     8461 gtgcgcgcct tcggcctgct ggcgaccatc gtggccatgt tcttcgtccg cggcagcgag
     8521 accgaggacc cgatgaacat gctgaaccgc ggctactggg tcacgaccat cctctcggtc
     8581 gcggccctcg cgctgaccac gttcgtcatg atgaacaccg gcggggagac gccggcgaac
     8641 ggcattccgc cctgggtctg gttcttcggc gccggcgtgg tcggcctcgc gacgagcgtg
     8701 gccttcgtct acatcacgca gtactacacc gccggcgcgt ggcgcccggt caaggagatc
     8761 gccacggcca gcaagaccgg cccggcgacg aacatcatca gcggcaccgc cgtcggcttc
     8821 gagaccaccg cggtcaccgc catcaccatc ggcatcgcgc tgttcgtcag ccactggctg
     8881 ggcgagcagg ccggcctgat ccagaccctg gacgacggca cgacgcgcaa cgtcggcggc
     8941 atcttcggca cggccgtggc cacgatgggc atgctcatga cgacggcgta catcctcgcc
     9001 atggacacct tcggcccgat caccgacaac gccggtggca tcgccgagtt cagcaaggcc
     9061 gagggcggcg cgcgcgagat cacggaccgc ctggacgcgg tgggcaatac gacgaaggcc
     9121 ctcaccaagg gctacgcgat cgcgtcggcc tcgctggcgg ccttcctcct gttcagcgcc
     9181 tacatcgaca aggtcaatct gatcctgacg cgtcagatcg agagcggcat ccgcgaggcg
     9241 ggcacgctgc tcgaggccgt cgacctcgcc gacgtgaacg tcttcatcgc cgccctcatc
     9301 ggcgcgatgc tcgtctactt cttctcgagc ctggccatcc gcgcggtggg caccgccgcg
     9361 caggcgatca tcgtcgaggt ccgacggcag ttccgcgaga tgccggggat catggactac
     9421 acccagcgcc cggactacgc gcgcgtcgtg gacatcacga cccgcgccgc gctgcggcag
     9481 atgatcctgc cgggcacggt ggcggtcgcg accccgatca tcgtcggcct cctcctgggc
     9541 cacgaggcgg tcgcgggatt gctcatggtg ggcaccatcg ccggcgtcct gctggccacc
     9601 gtgctgaaca acggtggcgg cgcgtgggac aacgcgaaga agttcatcga ggccggccac
     9661 ctgaccgatg acgccggcaa cgtgctgggc aagggctccg acgcccatgc cgcggccgtc
     9721 gttggcgaca cggtcggcga cccgttcaag gacacggcgg gaccgtccct ccacgtcctc
     9781 gtgaagctgc tggcgacgat caccctggtg ctcgcgccgc tcttcatccg gtagcaggtc
     9841 cgatggccgc cggcgtcacg ccggcggccg catcggtctc atggacctca tcatccaggc
     9901 cgcactggtc ggcctcctcc agggcctgac cgagttcatc ccgatcagct cctccgcgca
     9961 cctcgagctg gcgccgtgga tcgctggctg gggctcggac gggctgatcg gcagcctcgc
    10021 cttcgacgtc ttcctgcacc tcggcacctt cgtcgccctg gtcgcctact tctggcgtga
    10081 ctgggtgcgc tacatcgccg cctggttcgc ctccgtgcgg gaacgtcgca tcggggagga
    10141 ttcggatcgg cgcatcgcgt ggctgctgct cgccgccacc atcccggccg cgatcatcgg
    10201 cttcgcgctc gaggacttca tcaccgaggc gttccacggg gacagcgacg gcgcacggct
    10261 ggcgatcgcc ggcttcctgg tcatcggggc gaccgccctc tggctcgcgg accgatacgg
    10321 gcgccggcag cgcccgatgg agaacctctc cacgggctcc gccctcgtca tcggcttcag
    10381 ccaggccctc gcgctcttcc ccggcatcag ccggtcgggc gcgaccatca ccgccggcct
    10441 ggccctgggc ctcacgcgcg aagcggcggc acgcttcagc ttcctgctgg ccaccccgat
    10501 caccttcggc gccggcgtgt acggatcgcg gcacctgctg accgaggccc acacctcgtc
    10561 ggagtgggtg gccatcggcg tcggcttcgc ggtcgccgcg ctgtccggca tgctcgcgat
    10621 cggcttcctg ctcgcctggc tgcggcgtcg ttcggttgcg atcttcagca tctatcgcat
    10681 cgcgttcgcc gccctggtgg tggtcctggt cgtcctcggc cgctgacccg tcatcgccag
    10741 gtcggcgggc gaccgagcgg agtcgagcaa cctttgactt ccccgggggc ccgacctaca
    10801 atgcggcggc cgccgggagg accggccggc atgcctggcc cgcatctctc gagattgggg
    10861 gtacggccag cttcaggaga cgccgcccca ggtcgtttcg acgtcgggcg gggcctggag
    10921 cgttttttgt tatcagcagc agacgtcgtc ggtcggtaac cccaaccggc cgcatgcccc
    10981 caaccggggg acgtcgcagg aggacgaggg gacgtacgcc atgaacacca agccggtcta
    11041 tctcaccgcc gagggtctcg agaagctcaa ggccgagctg aacgagctcg tgaccttgga
    11101 gcgtccgcgg gtcgcggcgc gcatccacga ggcgaagctc gacggtgacc tcagcgagaa
    11161 cgccgagtac gaggacgcca agcaggagca gtcgttcctg gagggccgga tcgccacgct
    11221 cgagctccag ctgcgcaacg ccgcggtcat cgagaagacc aatggcgact cggtcggaat
    11281 cggctccaag gtggtcatca agggcagcga cggtgaggag accttcacca tcgtgggctc
    11341 ggcggaggca gcgccccgcg acggccggat cagcaacgag tcgccggtcg gcgtcgccct
    11401 catgggccgc aagaagggcg acaaggtcgt cgtcaatgcg ccctccgggc cgatcgagta
    11461 cacggtcgtc cgcgtcggct agggacgcac gtgttctggg ctgacgagct cgccgcgtcg
    11521 gccaccggcc gccaggtcgt caacgactcg aagacgccgt ccgggacggt tcacgtcggc
    11581 agcctgcgcg ggccggtggt cgtggacgcc atcgcccgcg ccctccgcga cgcggggcgc
    11641 gaggtcgagc ttcgctacgg cgtcgacgac ctcgatccga tggacgccca ggcgctgctc
    11701 accccggacg ccatcgaccg atacatgggc gtgccgctcg cccacgtccc ggatcctgcc
    11761 ggcgactgcc atgcgtccta cgcccgccac ttcgccggcg tcttcgtcga caccttcgac
    11821 ggcctgggca tccgaccgga cacctactac tggatgagtg aggtctacgc ctccggcgcc
    11881 atggaccgat acatccgcgc cgcgctggac gccgccccca ccatccgcga gatctaccgg
    11941 cgcgtcgcca aggtcaacca cccggacgag tggcacccgc tgttcgtcat ctgcgagtcc
    12001 tgcgggcgca tcgggacgac catcgtcacc ggctgggaca cggagcgcgg cgtcgtccgc
    12061 tacgagtgcc gcacggactt ggtgcgctgg gccaccggct gcgggcatgc cggcgaggtc
    12121 tcgccgttcg gtggccgcgc caagctgccg tacaacgtgg actgggcggc caagtgggac
    12181 ctgttcggcg tcaccatcga gccggccggc aaggacctct ccaccgccgg agggtcgcgc
    12241 gaccgctccg acgccatcgc gcgcgaggtg ttcggcaacg agccgccccg caacctgccg
    12301 tacgagttcc tcaacatcgg cggacggaag atgtcgacct cgaagggcgt cggcgcggcg
    12361 gcaacggaga tcgcggacgt catcccgccg gagcagctgc ggctcctctt cctgcgaccg
    12421 cgccccaaca ccgcgatcga gttcgacccg tacgggaccg atgcgatccc gcgcctcttc
    12481 gacgaatccg atcgcctggc ggccgccacc gccggtcgcg aggtccgcgg cgagctcccg
    12541 cccgaccacg aacgcgtctt cgcgttgacg caggtggacg cggatgccga tgtggccgcc
    12601 ctggccgccg cctaccgacc ggcgttcagc cacctggcgc tgctggagca gatcccgggc
    12661 gtcgacgtcg aggaacgcgt ggccgtcgag aagggcggtc cgctgacctc ccacgaacgg
    12721 gacctgctgg aggagcgccg gcgcgcagcc cgcgcctggc tggaggcgta tgcgcccgag
    12781 cgcgcgcgtc tggcggtcca gcgtgacgcg ctgccggccg cggccgccga gctcgacgac
    12841 gcgcagcgtg cctacctcgc cgcgctggcc gacgcgctcg ccggcgtgga gtggagcggc
    12901 gaggcggtgc aggcggccat cttcgccacc gcgaccgcta acgacctccc ggccggccgg
    12961 gccttcgcgg cgctgtacgc cgccttcctc ggccgcacgt ccgggccgcg cgccggctgg
    13021 ctgctggccg cgctggagcg tgacttcgtc atcgggcgca tccgcgacgc cgcgtcggcg
    13081 gccagggtgg cgtcatgagc gtcgggctcc agcgcctccg cgacgacgcc gatgctctcc
    13141 gtcgtgcggc gaccgacaag ggcgaggacc ccgccctcgt cgacctggcc gtcgcgctgg
    13201 acgagcgtcg gcgcgtgctg ctggccgagg gcgatgccct caaggccgag cgcaacgcga
    13261 gctcgaagcg catcggtgag gccattcgcg gcggcgccga ccccggaggg cccgacgtcg
    13321 ccgacctcaa ggtgcgctcg acggcggtgg gggagcggat cagggcggtc gacgaggagc
    13381 tggcccgcgt ggaagcggag ctcgacgacg ccatgctgcg catcccgaac ccggcggatc
    13441 ccgacgtccc gatcggcggc gaggaggcga acctcgtggc gcggtcgtgg ggcgaggtcc
    13501 aggcgcgggt cgacgactcg gaccccgagt acggcccgtg ggagcgccga ccgcactggg
    13561 agctggccga gtcgctcggg atgctggaca acgcccgcgg cgcgaaggtc gcgggatcgg
    13621 gctttcccgt ctacctcggc gcggggtcga acctccagcg cgcgttgatc aactggttcc
    13681 tggacgtcca cacccgcgag cacggcatga ccgaggtgtg gccacccgtc ctggtcaaca
    13741 cggactcggc gcgcggcacc ggccagatac cggacaagga ggaccagatg tacgtcgtca
    13801 cccgtgacga cctgtacctg atcccgaccg cggaggtccc cgtcaccaac ctccaccgcg
    13861 acgagatcct ggacggcgcc atgctgccgc tccgctatgc ggcctactcg gcctcgttcc
    13921 gccgcgaggc gggcgccgcc ggcaaggaca cgcgcggcat cctgcgcgtc caccagttcg
    13981 acaaggtgga gatggtgagc ttcacccgcc cggaggagtc cgccggcgag ctcgagtggc
    14041 tgaccgagcg ggccgaggtg ctcctccagc ggctggggct gacctaccgc gtgctcctca
    14101 tggcgacccg ggagatgggc ttcacccagg cccggaagta cgacctcgag gtctgggctc
    14161 ccggcgtcga gcgctggctc gaggtcagca gcgtcagcaa cttccgcgac ttccaggcgc
    14221 gccgcatggg gatccgattc cgtcccgacg ccggagcgcg cccggagctg gtccataccc
    14281 tcaacgggtc cggcctggcg ctcccgcgga cggtcgccgc catcctcgag acgtaccagc
    14341 ggccggacgg atcggtcgag atccccgagg tgctgcggcc ctacttcggc ggccaggagc
    14401 tcatcggtcc aggcttgagt gcctgagccc tcggcgaggt gatccggcgc gtggatcggc
    14461 gcgtaccctc gtcaccatgt cgattcctgc gctgctcgcc accttcggcg tcatggccgt
    14521 cctcgacggc atctggctga cgctggtcgc ccgaaacttc taccgcgaca acatcgggca
    14581 cctgctcggc gacgcgacga actggccgcc ggcgatcctc ttctacgtca tctacaccat
    14641 cggcgcctgg ttcttcgcca cgcagccggg cgtggacgac ggctccgcga tcacggccgc
    14701 cctgcggggt gccgccttcg gcttcgtggc ctacgcgacc tacgacctga ccaaccacgc
    14761 caccctgcgc ggctggcctt cgttggtcac cgtcgtcgac atggcgtggg gcacgatcct
    14821 cacggcaacg gtggcgggcg tcgccgcctg gatcacgctg acgttcctcg cctcctgaga
    14881 ccgatgcgcc cgccgctgcg ggcgacgatc acgccgccga tgcacagggc gccgccggcg
    14941 aacgccagcg ctggcggcac ctcgccgagc agcagccagg ccatcacgat cgccaccggc
    15001 ggtacgagat aggtggtgct gcccaaccgc ccggccgtcg tgcggttcag cgcgaaggcc
    15061 caggtcgtga aggcgatggc ggtcgggaag atgccgaggt acacgatcca ggcgatggcg
    15121 tccgcgggcg ccgtccccag ctcctgcacc agcagcgggc cgaacggcag cgtcgcgatc
    15181 gcgcccacgg tgcagccgat ccacgtcacc gtcagcgccg gcacccgctt gagcgccggc
    15241 ttctgcaggg tcacgccgag cgcgtaggcg agggccgcca ccaggcacag gagcacgccg
    15301 atcggcgccg atgcctcgcc cgacgccgtc ccgctcgcga cgccgatgac gagcgtgccg
    15361 gcgaacgcaa cggccgaccc ggccaccagc cgcggcggga agccctcgtg caggaagatg
    15421 cccgcgaacg cggcgatgag gatcgggccg atgttgacca gcatggcggc cgtcccggcg
    15481 tccaccagcc cctcgccggc gttgagcgcc acgaagtacg tgccgaacca gacgacgccg
    15541 gccgccgcga tcaggagcca gtcacggcgc cgtcccggga gtgccggacg ccgcagcgcg
    15601 accagcactc cgagggccag cgcgccgacc agcagccgcc ccagcgcgaa ggagccgggc
    15661 gacaggtcgt cggccacggc gcggatcccc acgaaggccg aggcccacag caggacggtg
    15721 aggagcgccg cgccgatcgc gagccagtcg atgctggcgg ccgggacccg tggctggtcg
    15781 aggatggtgg cggtcgtcgt atccgtcatc ggtgcgtgga acgctggacc cctgttcggt
    15841 gtgagatcgg gaaccggata cgatagcgac ggccgcggag gggtggcaga gcggacgatt
    15901 gcagcggtct tgaaaaccgc cgtggggcaa cccaccgtgg gttcgaatcc caccccctcc
    15961 gcca
//
LOCUS       DASTYP010000018         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_911796, whole genome shotgun sequence.
ACCESSION   DASTYP010000018 DASTYP010000000
VERSION     DASTYP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911796"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VFV59_00915"
     CDS             <1..1519
                     /locus_tag="VFV59_00915"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5324426.1"
                     /translation="VYTQVDPGQTEDGNLANAAALIDQWGIGRAGFDDGLVLMVGLEP
                     DLIHGKASLYGGSGFISQHIDEDGLQSIIDDRFIPKARDGDLNAAALDAIEAVQERMA
                     PDTVPLTVGRVLNAVLGLIVSPLLLLGIVGWAYLRWRREGDDPDLVDSPSVLMAGPPA
                     EMTPPLATVVRNGTADDHSIATLLAELASTGRLGFQNLDRVREVKRDDDPDPLTDPAI
                     EVRTDAAADDERLPRPEREAWQILQRKGSTSGILSRERLWSLNDSMKPVRRSLEEEAV
                     RIGWLTRLPGPAIQRMTWTGVGIAVIGGGAIFLGVTVPMSGAVMLGGALILAGVAVIG
                     FGRAMSQRTQNGAYVEMMLRAYRRTLRKTMDQARSMGEVVEQPEVARLADTPDKAVVW
                     GIALGLHDEVAKVLARGLEHQAQRTGSPAGAYYPYWMGSSPGSAWSGAAGDMAGGVSY
                     GSGSIFSASAIPDIGSMFDALTSVGSTPPSSSSSSGGGGFGGGGSGGGGGGSGSF"
     gene            1818..2420
                     /locus_tag="VFV59_00920"
     CDS             1818..2420
                     /locus_tag="VFV59_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324427.1"
                     /translation="MQLRLDPRRLSALKRLAGEAGTRPGELARQWVEERIDAARTGGD
                     RSLAEQVADLVARVEALEGGRPAPVSQPQSAPEPEPEPQPDAMAAAPEQPMQEAQAPE
                     AFAPEPVAEAPRKRARRNTVAAAPSERVALHDEMIAVLRERGPMSAGELASAIAERGR
                     YAPPRSGKPLDAAMVSQRVSNPTYRSRFTRQGGQIGLAEA"
     gene            complement(2481..2684)
                     /locus_tag="VFV59_00925"
     CDS             complement(2481..2684)
                     /locus_tag="VFV59_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEU5324428.1"
                     /translation="MAVAKVTELSCTSTESFEDAVRQGVDRAQKTLRGVRSAWVKEQR
                     VNIGRDGAVEFQANILVTFVLED"
     gene            complement(2765..>3060)
                     /locus_tag="VFV59_00930"
     CDS             complement(2765..>3060)
                     /locus_tag="VFV59_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5324429.1"
                     /translation="FDPDIVFAAAARFDKAIEVNSRPERLDPPKRLLRLAVEAGCRVA
                     IDSDAHTPGQLFWLGNGCERAYLCGVTVPMIVNALPLDELQAWTAAHDSAGGS"
BASE COUNT          450 a         1086 c         1120 g          404 t
ORIGIN      
        1 ggtctacacc caggtcgatc ccggccagac cgaggacggc aacctggcga acgcggccgc
       61 gctcatcgac cagtggggta tcggccgcgc cggcttcgac gacgggctgg tgctgatggt
      121 ggggctggag cccgacctca tccacggcaa ggccagcctg tacggcggct cggggttcat
      181 cagccagcac atcgacgagg acgggctgca gtcgatcatc gacgatcggt tcatcccgaa
      241 ggcacgtgac ggtgacctca acgcggccgc gctcgacgcg atcgaggcgg tgcaggagcg
      301 catggccccg gacacggtgc cgctgaccgt cggccgggtc ctcaacgcgg tgctcgggct
      361 catcgtgtcg ccactgctcc tgctgggaat cgtgggctgg gcctacctgc gctggcggcg
      421 cgagggcgac gacccggacc tggtcgactc gccctcggtg ctcatggccg gcccgcccgc
      481 cgagatgacg ccgccgctgg ccacggtcgt gcgcaacggg acggcggacg accactccat
      541 cgcgacactg ctggccgagc tcgccagcac cggccgcctc gggttccaga acctcgaccg
      601 cgtccgggag gtgaagcgcg acgacgaccc cgacccgctc accgatcccg ccatcgaggt
      661 tcgcaccgat gccgccgccg acgacgaacg cctcccgcgt cccgagcgcg aagcctggca
      721 gatcctgcag cgcaagggca gcacctcggg gatcctcagt cgcgagcgcc tctggtcgct
      781 caacgacagc atgaagccgg tgcgacgttc gctggaggaa gaggcggtgc gcatcggctg
      841 gctgacgcgg ctcccgggcc ccgcgatcca gcgcatgacg tggaccggcg tgggcatcgc
      901 cgtcatcggc ggcggcgcga tcttcctggg cgtcacggtc ccgatgagcg gtgcggtgat
      961 gctgggcggg gcgttgatcc tggcgggcgt ggcggtcatc gggttcggtc gggccatgag
     1021 ccagcgaacg cagaacggcg cctacgtcga gatgatgctg cgggcctacc ggcgaacgct
     1081 gcgcaagacc atggaccagg cgcgcagcat gggcgaagtg gtcgagcagc ccgaggtggc
     1141 acgcctggcc gatacgccgg acaaggccgt ggtgtggggc atcgccctgg gcctgcacga
     1201 cgaggtcgcg aaggtcctcg cccgcgggct cgagcaccag gcgcagcgca ccggctcccc
     1261 cgccggcgcc tactacccgt actggatggg ctcgtcgccg ggatcggcgt ggtccggcgc
     1321 ggccggggac atggcgggcg gcgtgagcta cggctccggc agcatcttct ccgcctcggc
     1381 catccccgac atcggctcca tgttcgacgc gctcacgagc gtggggtcga cccctccctc
     1441 cagctcgtcg tcgagcggtg gcggggggtt cggcggcggc ggcagcggtg gcggcggagg
     1501 cggatcgggc tcgttctgac tccgcactgg cgcattcgtt taaacgagcg tatcgtttaa
     1561 acgtgagttt caaccgacgc ggcggagatc gccgccccga ccgacccgaa cgcttcgacc
     1621 gcgatcgggg ggagcggccg agctatccac cccgcggcgg agatcgcccg ccgtaccagc
     1681 gcggcggagg tggcgctgac cgcccgccct atcagcgcgg cggaggtggc ggtgaccgcc
     1741 cgccgtacca gcagcgtggc ggcgaccgtc cgccctacca gccccgaccg cgattcaacc
     1801 ccgaggacga gggcgccatg cagctgcgcc tggacccgcg tcgtctgagc gcgctcaagc
     1861 ggctcgccgg tgaggcgggc acgcggcccg gtgagctggc ccgtcaatgg gtcgaggagc
     1921 ggatcgacgc ggcccgcacc ggtggtgatc gctcccttgc ggagcaggtg gcggacctgg
     1981 tcgctcgcgt cgaggcgctc gagggaggcc gaccggcacc ggtgagccag ccgcagtccg
     2041 cgcctgagcc ggagcccgag ccgcagccgg acgccatggc cgcagctccc gagcagccga
     2101 tgcaggaggc gcaggcacct gaggcgttcg cgcccgagcc cgtggccgag gcgccacgca
     2161 agcgtgcgcg gcgcaatacc gtcgcggccg cgccgagcga gcgggtcgcg ctccacgacg
     2221 agatgatcgc cgtcctgcgc gagcgcggac ccatgtccgc cggtgagcta gcctcggcca
     2281 tcgccgaacg cggccggtac gcgccgccgc gcagcggcaa gccgctcgac gcggccatgg
     2341 tcagccagcg cgtcagcaac ccgacctacc ggtcgcggtt cacgcgacag ggcgggcaga
     2401 tcggactcgc ggaggcctag ggaccgggca gcagaacggc cggcgcgcga ggcgccggcc
     2461 gtatcggtcc agcggggatg tcagtcctcg aggacgaacg tgaccaggat gttcgcctgg
     2521 aactctacgg cgccatcccg gccgatgttc acgcgctgct ccttgaccca ggcgctgcga
     2581 acgccgcgca gcgtcttctg tgcgcgatcg actccctgac gaaccgcatc ctcgaagctc
     2641 tcggtcgagg tgcaggacag ctcggtcacc ttggcaacgg ccatcggatt ctcctcatgc
     2701 gggtgggcgt ccagtatgcg cccgacctga ccgatgcacg tcgcgcgcca tccaggcgcc
     2761 gcggtcagga gccgcccgcc gagtcgtgcg cggccgtcca ggcctgcagc tcgtccaggg
     2821 gcagggcgtt gacgatcatc ggcaccgtca cgccgcagag gtaggcgcgc tcgcagccgt
     2881 tgccgagcca gaacagctgc cccggggtgt gcgcgtcgga gtcgatggcg acccggcagc
     2941 cggcctccac ggccaggcgc agcagccgtt tcggcggatc gagccgctcc ggccgcgagt
     3001 tcacctcgat ggccttgtcg aatcgggcgg cggcggcgaa gacgatgtcg gggtcgaacg
//
LOCUS       DASTYP010000019         6472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_766159, whole genome shotgun sequence.
ACCESSION   DASTYP010000019 DASTYP010000000
VERSION     DASTYP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766159"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(568..1683)
                     /locus_tag="VFV59_00935"
     CDS             complement(568..1683)
                     /locus_tag="VFV59_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5324430.1"
                     /translation="MTMTEPTNTPLSRAETLPSRYYLDPDVLELERERIFGRTWQLVA
                     RMDELARVGDFVPVTVVDEPIVITHAQDGELKAFYNVCRHRAGQVALTKGNRKSLQCR
                     YHGWTYGLDGTLRACPEMEDTENFRKEDFGLIPVRVERWGPFVFVNLSADAPPLREVM
                     GAIPAEVERSGYDVDRMRLVERRDYVIECNWKVYVDNYLEGYHLPIAHPQLYKELDYD
                     SYRVEEFRYYSKQHAPIRELRPGEELGRDRRYLRTADGEEDALYYWLFPNTMFNIYQD
                     NMSSNVILPLGPDRTLTIFEWFFAEPGSGPGWESMQQTIAFSDEIQQEDIVICEQVQR
                     GLRSRAYDTGRFSAKRENGVYHFQNLVREFLGDQIAG"
     gene            1807..2793
                     /locus_tag="VFV59_00940"
     CDS             1807..2793
                     /locus_tag="VFV59_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5324431.1"
                     /translation="MNHLRRIALLPVAMLILAACQAGGNGGQSAGESAAESAAESGGA
                     SQPAASGATGGPVCDDYSGDDYLGRICEAGVIVVSTDPEYPPQSYADGDTFAGFDIDV
                     ATEIANRLGVDIEWETPGWEAITAGNWSGRWDMSVGSMTITEDRKEIIDFTQPYYYTP
                     AQMATYSGSGIASVDDLAGETVCVGGGTTYLQWLDGTLTLTEEAGDVTEPPEGVQSTT
                     FDTDINCAEDWRAGRTNFAGWLSSSTTVEGAIANGFEIEKVGDPVFYEPLAVAFDNTV
                     EDNDSLVEAVDEIIGQMHEDGTLSELSRHWYCADGTYPCEDESLALDLTVRD"
     gene            2869..3681
                     /locus_tag="VFV59_00945"
     CDS             2869..3681
                     /locus_tag="VFV59_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5324432.1"
                     /translation="MIESRVEDRPRRYDIEDVERAEQRARWSWRAIFAVVWIGLVLGL
                     GIGLALTGRIDPGFIAEVGPFILEGTPITLLVCVVSISLAIILATLGALGRLSNNPVL
                     NAIASFYVSLIRGTPLIVQILFWYLALPQFGIVLDALPAGIGALAFNYGAYMTEIFRA
                     GVEAVPPGQREAARALGMTEAQVMRRIVFPQALRIVTPAIGNEFIAMIKDSALVYILG
                     VREVLWRADVTGTRFFRSTETLLIAAVVYWVLTIALSFAQERLERRLRRGER"
     gene            3687..4478
                     /locus_tag="VFV59_00950"
     CDS             3687..4478
                     /locus_tag="VFV59_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU5324433.1"
                     /translation="MTPPDAPQLGEAVVRVTGLEKYFGRNHVLRGIDLEVRQREAVMI
                     IGRSGSGKTTLLRCINFLEEPTSGSVDVDGLRVDADPLHARSRRHREQIRQIRLRTGM
                     LFQDFNLFPHMTALGNCIEAPMRVRRIPRAEAVERADYFLEVVGLADKRNEYPARLSG
                     GQKQRVAIARALAMEPKVLLFDEPTSALDPELIGEVLVVMEDLAHSGTTMIVVTHEMH
                     FANEAADRVVFMEEGEIVEQGPPEQILDSPREERTQRFLRRFLKT"
     gene            complement(4493..4963)
                     /locus_tag="VFV59_00955"
     CDS             complement(4493..4963)
                     /locus_tag="VFV59_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324434.1"
                     /translation="MITKRTKRRPVEPADPDLTVTPSADDAATAATVPAASVAPIDDD
                     QLDAAILRFIRQKPNRQVDLSPLADELGVDPFRVQLAAERLGRRRMIVLPFVEPGTAG
                     GAELTAVGLRWLLAREGGTPADQPVALKPARKRVRPEEEAARLPRSQVYGVLRG"
     gene            5004..5891
                     /locus_tag="VFV59_00960"
     CDS             5004..5891
                     /locus_tag="VFV59_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU5324435.1"
                     /translation="MAEIDPYTVLGVSRTAMRTDIARAYRALARRHHPDAGAPQSAEM
                     ARINEAWHILSDVNRRARWDRLHAQPTVAGPHWSPAERVAGPMRRPPPRPAAPPSALD
                     SGWAAAIVVLGALVLVGGVMVALSLAAAPTDDRIRFADPEITFLRPPDWVVAAGDPDD
                     PPAHRVVAHLVTFGLDGDQLCTSFGTVCPLARDDIPSGEASIVVTAYEGATPPVPDPV
                     VSRPFGLDADAMIDGKPAAFEQQVVDERTTLLWWQLSPPGFPERWIEISAVVRGLSLD
                     QADVIAEIDALLETVELPG"
     gene            5994..6308
                     /locus_tag="VFV59_00965"
     CDS             5994..6308
                     /locus_tag="VFV59_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324436.1"
                     /translation="MQYDLGLSGLGILVALALGFGVIAHVIAGRHTTWMWAVGALAAF
                     LGGLYFSEVLFATYTEDEIQPIIDGLALDESMLGGLLVGSAAVLVTWFLTQRRTHGPI
                     AH"
BASE COUNT          987 a         2241 c         2250 g          994 t
ORIGIN      
        1 cgtgaccgag cacgccgcgc tcttcgccag attctccaca cgcggtcgag ccgccctggc
       61 ctgggttcga gagccgatgc tcccggcccc gaccggcctg ctctggttcg ccgagcgggc
      121 ggatgcagga cgcctcgcca ggcgagcgca acgacgcccg tcgagccgtc cgaattcaca
      181 cctggtgtga gcgggtcgca gcgcatggcc gtgcggggct cccggacagg ccgaattccg
      241 ttgtggtgtg aattgggacg cttcagcgcg ccaatgacca tcctcgtcgc gccacatgca
      301 cgcttggtgt gaattgagac gcgtcctgcc cgtcgccgag ccgacatcga tcgccgaaag
      361 gcgcaaattc acaccaaggg gtgcgccgga ccgacccccg cggccgctcg ccgactccga
      421 agaggccgaa tgccgtcctg gtgtgaattg gaacgcgtca gcgcgccgat gaccggcctc
      481 gtcgcgccac atgcacgcct ggtgtgaatt gagacgcgtc agcacgccgg gacgatgtcg
      541 ccggtcaccg ctcgcaacgg ggcagcgtca ccccgcgatc tggtccccga ggaactcgcg
      601 gaccaggttc tggaagtggt agacgccgtt ctcgcgcttc gcggagaagc ggcccgtgtc
      661 gtaggcacgg gaccgcaggc cgcgctgcac ctgctcgcag atgacgatgt cctcctgctg
      721 gatctcgtcg gagaacgcga tcgtctgctg catcgattcc cagccgggac ccgagccggg
      781 ctcggcgaag aaccactcga agatcgtcag ggtccgatcc gggcccagcg gcaggatgac
      841 gttcgacgac atgttgtcct ggtagatgtt gaacatcgtg ttcgggaaga gccagtagta
      901 gagcgcgtcc tcctcaccat cggccgtgcg gaggtagcgg cggtcgcggc cgagctcctc
      961 cccggggcga agctcgcgga tcggcgcgtg ctgcttcgag tagtaccgga actcctcgac
     1021 ccgatacgag tcgtagtcga gctccttgta cagctgcggg tgtgcgatcg gcaggtggta
     1081 gccctcgagg tagttgtcga cgtagacctt ccagttgcac tcgatgacgt agtcgcgtcg
     1141 ctcgaccagg cgcatgcggt caacgtcgta gccggagcgc tcgacctcgg cggggatggc
     1201 ccccatcacc tcgcgcaacg gtggcgcatc ggccgagagg ttgacgaaga cgaagggccc
     1261 ccagcgctcg acgcgaaccg ggatgaggcc gaagtcctcc ttgcggaagt tctcggtgtc
     1321 ctccatctcc gggcaggcac ggagagtgcc gtccagtccg taggtccagc cgtgatagcg
     1381 gcattgaagc gacttgcggt tgcccttggt gagcgccacc tggccggcgc ggtggcggca
     1441 gacgttgtag aaggccttca gctcgccgtc ctgggcgtgg gtgatcacga tcggctcgtc
     1501 gacgacggtg accgggacga agtcccccac ccgcgccagc tcgtccatgc gcgcgaccag
     1561 ctgccacgtc cgcccgaaga tgcgctccct ctcgagctcg aggacgtcgg ggtcgaggta
     1621 gtagcgcgag ggcagggtct cggcgcggct gagcggcgtg ttcgtcggct ccgtcatggt
     1681 catgcagccg agtgtatcgc ccggcaaatc gggcggcacg acacggacga acgtgctaca
     1741 acacggcgac cgagcggatc gtaggcgccg acgtggtcgg cgaggtggag gcgtggagga
     1801 gggcgaatga accacttgcg acgcatcgcg ctcttgccgg tggcgatgct gatcctggcg
     1861 gcctgccagg ctggcggcaa tggcgggcag tccgccggtg aatccgcggc cgagtccgca
     1921 gccgagtcgg gcggcgccag ccagcccgcg gccagcggcg cgacgggggg tcccgtgtgt
     1981 gacgactact cgggcgacga ctacctcggc cgcatctgcg aggccggcgt catcgtggtc
     2041 tccacggatc cggagtatcc gccgcagtcg tatgcggacg gtgacacctt cgccggcttc
     2101 gacatcgacg tcgcgaccga gatcgcgaac cggctgggcg tcgatatcga gtgggagact
     2161 cccggctggg aggcgatcac cgcggggaac tggtccggtc gctgggacat gagcgtcgga
     2221 tcgatgacca tcaccgagga tcgcaaggag atcatcgact tcacgcagcc gtactactac
     2281 acgccggccc agatggcgac gtacagcggc agcggcatcg cgtcggtcga cgacctcgcc
     2341 ggggagacgg tctgcgtggg cggcggaacg acgtatctgc agtggctgga cggaacgctg
     2401 acgctgaccg aagaggcggg cgacgtcacc gagccacccg agggcgtgca gagcacgacc
     2461 ttcgacaccg atatcaactg cgccgaggac tggcgtgccg gccgcaccaa cttcgcgggc
     2521 tggctcagct cgtccacgac ggtggagggg gcgatcgcca acggcttcga gatcgagaag
     2581 gtcggcgacc cggtgttcta cgagccgctt gccgtcgcgt tcgacaacac cgtcgaggac
     2641 aacgactcgc tcgtcgaggc ggtggacgag atcatcggcc agatgcacga ggacggcacg
     2701 ctctccgaac tcagccggca ctggtactgc gccgatggca cctatccctg cgaggacgag
     2761 tcgctggcgc tcgacctgac cgtccgcgac tagcccagct tcgacacggg ccgggtgccg
     2821 gacagttccg gcacccggcc gccttcatct cgcagagaag gggccggcgt gatcgaatcc
     2881 cgggtcgagg atcgcccgcg tcgctacgac atcgaggacg tcgagcgtgc agagcagcgc
     2941 gccaggtggt cctggcgagc catcttcgcg gtcgtctgga tcggcctggt cctcgggctc
     3001 ggcatcggcc tggccctcac cggtcgcatc gaccctggct tcatcgccga agtcgggccg
     3061 ttcatcctgg aaggcacgcc gatcacgctc ctggtctgcg tcgtctcgat ctcgcttgcc
     3121 atcatcctgg cgaccctggg cgcgctcggc cggctctcca acaacccggt cctcaacgcg
     3181 atcgcgtcgt tctacgtgtc gctcattcgc ggcacgccgc tcatcgtcca gatcctgttc
     3241 tggtacctgg cgctgccgca gttcgggatc gtgctggatg cgctgccggc cggcatcggg
     3301 gcgctggcct tcaactacgg cgcctacatg acggagatct tccgcgccgg cgtcgaggcg
     3361 gtgccgcccg gccagcgtga ggcggcccgc gcgctgggca tgacggaggc ccaggtcatg
     3421 cgccgcatcg tcttcccgca ggcgctgcgc atcgtgacgc cggcgatcgg caacgagttc
     3481 atcgccatga tcaaggacag cgcgctggtc tacatcctcg gggtccgcga agtgctgtgg
     3541 cgggccgacg tcaccggcac ccgcttcttc cgctcgaccg agacgctgct catcgccgcg
     3601 gtcgtgtact gggtgctgac catcgcgctc agcttcgcgc aggagcgcct ggaacggcgg
     3661 ctcaggcggg gcgaacgatg acggccgtga caccgcctga cgcgccgcag ctcggcgagg
     3721 ccgtggtccg cgtgacgggg ctcgagaagt acttcgggcg caaccacgtc ctgcgcggga
     3781 tcgacctcga ggtccgccag cgggaggccg tgatgatcat cggtcgttcc ggctcgggca
     3841 agacgaccct cctgcgctgc atcaacttcc tggaggagcc gacgtccgga tcggtcgatg
     3901 tggacgggct gcgggtcgac gctgatccgc tccacgcgcg ttcgcggcga caccgcgaac
     3961 agatccggca gatccgtctc cggaccggaa tgctgttcca ggatttcaac ctgttcccgc
     4021 acatgaccgc gctgggcaac tgcatcgagg cgccgatgcg ggtgcgtcgg atcccgcgtg
     4081 ccgaggcggt cgagcgcgcc gactacttcc tggaggtcgt cgggctcgcg gacaagcgca
     4141 acgagtatcc cgcccgcctg tccggggggc agaagcaacg agtggcgatc gcccgtgcgc
     4201 tggcgatgga gccgaaggtg ctgctcttcg acgagccgac ctcggcgctc gaccccgagc
     4261 tgatcggcga ggtgctggtc gtcatggagg acctcgcgca cagcggaacg accatgatcg
     4321 tcgtcaccca cgagatgcac ttcgcgaacg aggccgccga ccgcgtggtg ttcatggagg
     4381 agggcgagat cgtggaacag ggaccccccg agcagatcct ggactcgccg cgcgaggaac
     4441 gaacgcagcg gttcctgcgg cgcttcctca agacctgagc gttcccggtg gatcagcctc
     4501 gcaggacgcc gtagacctgg gatcgcggca gtcgcgccgc ttcctcctcg ggccgcaccc
     4561 gcttcctggc gggcttcagc gccaccggtt gatccgccgg cgtgccgccc tcccgcgcca
     4621 gcagccagcg caggccaacg gcggtcagct cggccccacc cgcggttccc ggctcgacga
     4681 acggcagcac gatcatccgg cgtcgcccga gccgctcggc ggcgagctgg acacggaagg
     4741 gatcgacgcc gagctcatcg gccaacggtg agaggtcgac ctggcgattg ggcttctggc
     4801 ggatgaaccg caggatcgcg gcgtccagct ggtcgtcatc gatcggggcg accgatgcgg
     4861 ccgggaccgt ggccgcggtc gcggcatcgt cggcggaggg cgtgacggtc agatccggat
     4921 ccgccggctc gaccgggcgt cgcttggtgc gcttggtgat cacgggcgac aggatagcaa
     4981 gggccaccta cactgcggcg gccgtggccg agatcgatcc gtacaccgtc ctgggcgtga
     5041 gccgcaccgc gatgcgcacc gacatcgcgc gggcgtaccg cgccctggcg aggcgccacc
     5101 accccgacgc gggagcgccg caaagcgcgg agatggcccg catcaacgag gcctggcaca
     5161 tcctctcgga cgtcaaccgg cgggcccgat gggaccggct gcacgcgcag ccaaccgtgg
     5221 ccgggccgca ctggagcccg gccgaacggg tcgccggacc gatgcgacga ccgccacctc
     5281 ggcccgcggc accgccatcc gcgctcgact cgggctgggc ggccgccatc gtggtcctgg
     5341 gtgccctggt cctcgtcggt ggggtcatgg tcgccctgtc gctcgcggcg gctcccaccg
     5401 acgaccggat ccggttcgcg gacccggaga tcaccttcct ccgcccgccc gactgggtag
     5461 tcgccgcggg cgaccccgac gatcccccag cgcaccgggt cgtcgcgcac ctcgtcacct
     5521 tcgggctcga cggggaccag ctgtgcacct cgttcggtac ggtgtgtccc ctggcgcgcg
     5581 acgacatccc ctcgggcgag gcctcgatcg tcgtgaccgc ctacgagggc gcaactccgc
     5641 cggtccccga ccccgtcgtt tcgcggccgt tcggcctgga cgccgacgcc atgatcgacg
     5701 ggaagcccgc cgccttcgag cagcaggtcg tcgacgagcg cacgacgctc ctgtggtggc
     5761 agctcagccc gcccggcttc ccggagcgat ggatcgagat cagcgccgtc gttcgcggcc
     5821 tttctctcga tcaggccgac gtcatcgcgg agatcgacgc cctcctcgag acggtcgagc
     5881 tgcccgggta ggacgcgacc cgatgccatc cggcactggc gccgcccgca cgagcgggcc
     5941 agcgtagcgc gcgtgcgcgc gcatcggcgt gcgcggaacg gagggtccct acgatgcagt
     6001 acgaccttgg cctctccggc ctcggcatcc tggttgcgct ggcgctgggc ttcggcgtca
     6061 tcgcgcacgt catcgccgga cggcacacga cctggatgtg ggccgtcggc gccctcgccg
     6121 ccttcctggg cggcctctat ttcagcgagg tgctgttcgc gacgtacacc gaggacgaga
     6181 tccagccgat catcgacgga ctggcgctgg acgagtccat gctgggcggc ctgttggtgg
     6241 gaagcgccgc ggtgctggtc acctggttcc tgacccagcg acgtacgcac gggccgatcg
     6301 cccattgagc ctcaggctgg ccggtccccc gcctccggcc gcagctcgac cgcgaccgcc
     6361 acgccggcgg cgcggcgcag gtcgtcggcc agccgctcgt cccacgccac gcggtccggc
     6421 agccgaacct gccgggtcgc gccggccacc gagagcgaca gcacgaccgg ca
//
LOCUS       DASTYP010000020         9648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_839027, whole genome shotgun sequence.
ACCESSION   DASTYP010000020 DASTYP010000000
VERSION     DASTYP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839027"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFV59_00970"
     CDS             <1..274
                     /locus_tag="VFV59_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225175.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5324437.1"
                     /translation="GWRSHVNLIPLNPTPGSRWSGTSPAGIARFVAILRGGGVAVTVR
                     DTRGREIEAACGQLHAQLRGKSLRDARQARDARQGQPTPEVAVASR"
     gene            274..972
                     /gene="rpe"
                     /locus_tag="VFV59_00975"
     CDS             274..972
                     /gene="rpe"
                     /locus_tag="VFV59_00975"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5324438.1"
                     /translation="MDRPHISASILNADFSRLGDEVRRADAGGVDSIHLDVMDGHFVD
                     NLTMGPVVVESLRSHSRLPFHSHLMIDNPLAYAERFAEAGSDLIVFHVEAEDAPDEVI
                     RAIEAAGRRPGIALNPETPAEAVLPYLERVDLVLAMTVHPGWGGQSFITEVLAKMRAL
                     RDEIQRRALSVEIGVDGGVKTDTIASAWDAGGSVLVVGSGLYSTDGDLAPTVAALRAA
                     ALGRANAAPDTERT"
     gene            1111..2613
                     /locus_tag="VFV59_00980"
     CDS             1111..2613
                     /locus_tag="VFV59_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEU5324439.1"
                     /translation="MTIVTQGTEAPVPGAEVSVEGRSASTGDAGTATLTAMRGGEVEV
                     TADGYDATTATVPDDGGLRVALRSNVVSGTITDEAGEPIAGVRVFVDGADTMVESDED
                     GAYALPGVPEEGTVVYKMPGYRLAELPIDDQMERDVAMQPFEARALYAPAAVFEAPGR
                     LDNFLDLMERTEANAMVIDVKETSGQLYYATDLPEAVEAGAVMDNPIFDLSELLPMLK
                     ERGIYTIARMVVMKDNTTAAARPELAVRNVATGEPWRDNIGGAWLDPSAPGVAEYIAA
                     IAGDLADKGFDEVQLDYIRFFSDGPYDLADTNLPNVQSFRLPAIQRVLRVVSTRLDTT
                     KAFLGADVFPISFIVPDDQGIGQRPEVIMPYVDYFCPMVYPSHYGPGVFGFPVPNEHP
                     YDVIDETLQIMNEQKEGLPVVIRPWIQDFGYGPFPPYTVDQIQAEMQAASDNGAKGWM
                     IWNARATFTEAALGPPREGEDSAPVTSVPPAPSEGAQPSGSPSSSPSSSP"
     gene            2618..3067
                     /gene="dtd"
                     /locus_tag="VFV59_00985"
     CDS             2618..3067
                     /gene="dtd"
                     /locus_tag="VFV59_00985"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU5324440.1"
                     /translation="MRLVVQRVTRASVSAHGEPLGEIGRGAVVLAGIGQDDNIAVVDR
                     MADKLIGLRYFEDEEGRTNRSLADAFGSLLVVSQFTLYADVRRGRRPGFTGAALPDQA
                     VPLLERFVERLRVSGVEVSTGRFGASMAVELVNDGPFTLVLDSDRDA"
     gene            complement(3183..3374)
                     /locus_tag="VFV59_00990"
     CDS             complement(3183..3374)
                     /locus_tag="VFV59_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HEU5324441.1"
                     /translation="MARVCDICGKRPITGWNPQSVGMNRKRALRRWLPNLQPMTVTRD
                     GKVAKVSACSRCRRTAQKV"
     gene            3458..5539
                     /gene="recG"
                     /locus_tag="VFV59_00995"
     CDS             3458..5539
                     /gene="recG"
                     /locus_tag="VFV59_00995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU5324442.1"
                     /translation="MQSSRPLAPDELDRPIRHAIPALARGASALSKLGITTPREALWY
                     LPFRYDDFSDLRTLDALVPDEKQSALVRVDAVRVEPGFGRRPQRVIAQLSDERGSAEA
                     IWFGRRYVERRLRVGDEVVVSGKVSQRGWRFQFTSPEFSPAGHESVHTARVVPVYHLA
                     AGVTQKRLREVLARILDVALPAVEDPLTDAERNGLPRLDVALQTAHFPEETADVTDAL
                     DRLAFDELYALQLTLAQARRAREHLVAPKIAVTHAELAEVVAALPFELTRDQASAVEA
                     IAHDLASERPMRRLLQGDVGSGKTAVAAVALASAVRAGWQAALMAPTEILARQHHHGL
                     APLLDALGVRAEFLSGSLTPARKREIHDAIAGGMAEVVIGTHAVISEAVEFPRLGLAI
                     VDEQHRFGVGQRAALQAKGSGGEPHLLALTATPIPRTLALTVYGDLAISTIRELPPGR
                     QPIRTEIRDRQALPKIEAFLASEAAEGRQSFVVVPLVTESESLQAASAEAEAERLQAA
                     LPELRVGLVHGQQRADARDATMTAFAAGDMDLLVATTVIEVGIDVPNASVMLIEDAER
                     FGLAQLHQLRGRVGRGPHRSFCILLSDVTEPLARERLEVVRGSQDGFAIAEADLRLRG
                     AGNLLGTRQSGLPPLRIASLFEPRHLRLAERARELADTVVGADPTLERRPGLARLQAD
                     FTRLEEEGDAA"
     gene            5539..6081
                     /gene="rsmD"
                     /locus_tag="VFV59_01000"
     CDS             5539..6081
                     /gene="rsmD"
                     /locus_tag="VFV59_01000"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU5324443.1"
                     /translation="MRVIAGTARGVPLHAPRGDATRPITDRVKETLFAILGDRVPGAR
                     VLDLYAGTGAIGIEALSRGAASADFVERDRHAVTALRSNLDRTRLSASASVHATDVER
                     FLGSCGKSTWDLATVDPPYETRDIVAPLRTLVPHLAPDAIVVVKHFWRTEVPVVDRLV
                     AVRQRRFGETMLTFLQLEAP"
     gene            6078..6587
                     /gene="coaD"
                     /locus_tag="VFV59_01005"
     CDS             6078..6587
                     /gene="coaD"
                     /locus_tag="VFV59_01005"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5324444.1"
                     /translation="MTRVAVFPGSFDPMTNAHLDVARRASGLYDALVIGVLNNPKKQP
                     LFDIHERVDLIRACVADLGAHVSVDAFDGLTVDFARRHGAQFIVRGLRAVSDFEAELQ
                     MALTNRKLAPEVDTTFLMTALEYGYVSSSLTKEVARFGGDITPMVPAPVAEALARRLG
                     GGARGEPRV"
     gene            6690..7151
                     /locus_tag="VFV59_01010"
     CDS             6690..7151
                     /locus_tag="VFV59_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEU5324445.1"
                     /translation="MLDQAAVLELIDQLRVSVPEEVRQARRVTEEATRITERARDEGD
                     AIVARAQEQAAQMLEERELVRAAQQRAAEIIEAAQAEARELRRGADEYAAGVLIRLEG
                     ECIKALTSIKRGIDMLDERHRVAPAGDGRQPAANDAIVVDAEDEAPATSRP"
     gene            7148..7711
                     /locus_tag="VFV59_01015"
     CDS             7148..7711
                     /locus_tag="VFV59_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5324446.1"
                     /translation="MTAFNVAGLLHEPPGSTRDIRLRDHYVTLGPDVELAGPLDADLR
                     LQRTNRGILVRGELRAPLRRVCGRCTEPYVDDVRVAVEEEFLPTLDPVTGVPVRTDGS
                     EDETTQWIDEHHEIELDGVFHDELALTEPMLPLCRPDCPGLCVECGERLDRGDHAHGG
                     PEIDPRLAGLAALLNRGDPGEDDPGRN"
     gene            7797..8000
                     /gene="rpmF"
                     /locus_tag="VFV59_01020"
     CDS             7797..8000
                     /gene="rpmF"
                     /locus_tag="VFV59_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5324447.1"
                     /translation="MGVPKRKVSKARQGERRAHLAISAPPLVECEHCHELHRAHHVCP
                     TCGWYNGREAVAIEPVTPTEGQR"
     gene            8007..9062
                     /gene="plsX"
                     /locus_tag="VFV59_01025"
     CDS             8007..9062
                     /gene="plsX"
                     /locus_tag="VFV59_01025"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU5324448.1"
                     /translation="MRIAVDAMGGDHAPREIVRGAIDYATASPDQVILVGDVPRLERE
                     IAAYGKGRPGNLDLVDAPEVIEMGEHPATALRSKRKASIVVATDLVRDGEADAVVSAG
                     STGATMAAAVFRLGRIEGIDRPALPAHMLTATGPVMLLDVGANVDSDPENLVQFAAMG
                     AIFAEHVLHVRNPRIGLLNIGEEPEKGDELARHAHEKLSTLDLHFVGNVEAHDMIAHR
                     ADVVVCDGFVGNVVIKFFEGITSFIFRALREDLQQGPIAPLALLALKPGFDKMKTRFD
                     YERYGGAPLLGVKGVSIVTHGRAKARMIENAIRVGSEAAHARVPSLIEQWTREHPALA
                     HRGVRSRLAARLHRERA"
     gene            9110..9508
                     /locus_tag="VFV59_01030"
     CDS             9110..9508
                     /locus_tag="VFV59_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HEU5324449.1"
                     /translation="MVARRLALLALFEDEFRPGSAVQALDRLASERDVADDVRAHARA
                     IVDGVVEHRSDLDARIAAEAPLIPVPELGRVERTILRSALYEVLYSAATPSGEPMHDA
                     VTLARIYAGDAARRLVNGVLGSVSRSGGGA"
     gene            9513..>9648
                     /locus_tag="VFV59_01035"
     CDS             9513..>9648
                     /locus_tag="VFV59_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5324450.1"
                     /translation="MTDGTTYERLKKIIVEQLGVDEEEVTPEASFVEDLNADSLDLVE
                     L"
BASE COUNT         1399 a         3454 c         3431 g         1364 t
ORIGIN      
        1 cggctggcgc tcccacgtca acctgatccc gctcaatccg acgccgggct cccgatggag
       61 cggcacgtca ccggccggca tcgcccgctt cgtcgcgatc ctgcgcgggg gcggcgtggc
      121 ggtcaccgtg cgcgacaccc gggggcgcga gatcgaggcc gcctgcggcc agctccacgc
      181 ccagctgcgc gggaagtcgc ttcgcgacgc gcgtcaggcg cgcgacgcac gccaggggca
      241 gccgacgccc gaggtcgcgg tggcgagccg ctgatggatc gcccgcacat ctcggcgtcg
      301 atcctgaacg cggacttcag ccgcctggga gacgaggtcc gacgcgccga tgcaggcggc
      361 gtggactcga tccacctcga cgtcatggac ggccacttcg tcgacaacct gaccatggga
      421 ccggtcgtgg tcgagtcgct gcggtcgcac tcgcggctgc cgttccacag ccacctgatg
      481 atcgacaacc cgctggcgta tgcggagcgc ttcgcggagg cggggagcga cctgatcgtc
      541 ttccacgtgg aggccgagga cgcgccggac gaggtgatcc gcgccatcga agccgcgggg
      601 cgtcgtccgg gcatcgcgct gaaccccgag acacccgcgg aggccgtcct cccgtacctc
      661 gagcgcgtgg acctggtgct ggcgatgacc gttcaccccg gatggggcgg gcagtcgttc
      721 atcaccgagg tgctggcgaa gatgcgagcc cttcgcgacg agatccagcg tcgcgcgctg
      781 tcggtcgaga tcggggtcga cggcggggtg aagacggaca ccatcgcatc ggcctgggat
      841 gccggcggaa gcgtgctggt cgtgggcagc ggcctgtaca gtaccgacgg cgacctggcg
      901 cccacggtcg ccgccctgcg tgccgccgcc ctcggccgcg ccaacgccgc acccgacacc
      961 gaaaggacct gaccgctgcc ctcgcgccgc ctgctcccgc tgctcgtcgt cgccctcctc
     1021 gccgtcgtcc tcgccgcctg cagcgacggt cccaacctgc cgttcgtccc gacgccggat
     1081 ccgacccccg agcagccgcg cgaggtcgag gtgaccatcg tcacccaggg gaccgaggcg
     1141 ccggttcccg gggccgaggt gtcggtcgag ggtcggtccg cctcaaccgg cgacgcgggg
     1201 acggccacgc tcaccgccat gcgcggcggg gaggtcgagg tcacggccga cggctacgac
     1261 gccacgacgg ccaccgtgcc ggacgatggc gggctgcgcg tcgcgctgcg ctcgaacgtc
     1321 gtctccggga cgatcaccga cgaggcgggc gagcccattg ccggtgtgcg cgtcttcgtc
     1381 gacggcgccg acacgatggt cgagtcggac gaggacggcg cctacgccct gcccggcgtg
     1441 cccgaggagg gcaccgtggt gtacaagatg cccggctacc gcctggccga gctccccatt
     1501 gacgaccaga tggaacgcga cgtggccatg cagccgttcg aggcgcgggc gctgtacgcg
     1561 cccgccgcgg tcttcgaggc gcccggacgc ctggacaatt tcctggacct gatggagcgg
     1621 accgaggcga acgccatggt catcgacgtc aaggagacgt cgggccagct gtactacgcg
     1681 accgacctgc ccgaggccgt cgaggcgggc gccgtcatgg acaaccccat cttcgacctg
     1741 tccgagctgc tgccgatgct caaggagcgc ggcatctaca ccatcgcccg catggtggtc
     1801 atgaaggaca acacgacggc tgccgcccgg ccggagctcg ccgtccggaa cgtggccacc
     1861 ggtgagccgt ggcgcgacaa catcggcggc gcatggctcg atccgtccgc gcccggcgtc
     1921 gcggagtaca tcgccgccat cgccggcgac ctggcggaca agggcttcga tgaggtgcag
     1981 ctggactaca tccgcttctt cagcgacggc ccctacgacc tggccgacac caacctgccg
     2041 aacgtccaat ccttccgcct gccggccatc cagcgcgtgc tgcgcgtcgt gagcacgagg
     2101 ctcgacacga caaaggcgtt cctgggcgcc gacgtcttcc cgatcagctt catcgtgccg
     2161 gacgaccagg gcatcgggca gcggccggag gtgatcatgc cgtacgtcga ctacttctgc
     2221 cccatggtct acccgtcgca ctacggaccc ggcgtcttcg gcttcccggt gccgaacgag
     2281 cacccgtacg acgtgatcga cgagacgctg cagatcatga acgagcagaa ggaagggctg
     2341 ccggtcgtca ttcgcccatg gatccaggac ttcggctacg gacccttccc gccctacacc
     2401 gtcgaccaga tccaggcgga gatgcaggcg gcgtccgaca acggcgccaa gggctggatg
     2461 atctggaacg ctcgcgcgac cttcaccgag gcggcgctgg gaccaccgcg cgagggcgag
     2521 gattccgcgc cggtgacctc ggttccgccg gcaccgtccg agggagcgca gccctcgggg
     2581 tcgccctcga gctccccctc gagctctcca tagaccgatg cgcctggtgg tccagcgcgt
     2641 gactcgagcc tcggtgtccg cccacggcga accgctgggc gagatcggtc gcggcgcggt
     2701 ggtgctggcc gggatcggtc aggatgacaa catcgcggtg gtcgaccgga tggccgacaa
     2761 gctcatcggc ctgcgctact tcgaggacga ggagggccga acgaaccgct cgctcgccga
     2821 tgccttcggc tcgctgctgg tcgtcagcca gttcacgctc tacgcggacg tgcgccgtgg
     2881 ccggcgtccc ggcttcacgg gcgccgcatt gcccgatcag gcggtgccgc tgctggagcg
     2941 gttcgtggag cggctgcggg tgagcggcgt ggaggtgtcg accggcaggt tcggcgcctc
     3001 catggcggtg gagctggtca acgacggacc gttcacgctg gtgctcgact ccgatcggga
     3061 cgcctgagcg cggctcagga tcagcgagtt gtcatccgtc caccgtggcg atgccaggcg
     3121 ctcgcgcacg cgaacgcccg cgtcggacga cgcgggcgca tcggttcagg ccggggagaa
     3181 ggtcagacct tctgggcggt gcggcggcag cggctgcagg cgctgacctt cgcaaccttg
     3241 ccgtcacgcg tgacggtcat cggctggagg ttgggcagcc atcggcgcag cgcgcgcttg
     3301 cggttcatcc cgaccgactg ggggttccag ccggtgatcg ggcgcttgcc gcagatgtcg
     3361 cagacgcgag ccatggtcga agggtctcca atcacacgaa aaacccggcg ctcgtcgcgc
     3421 cggagcgacg gcgatggtag cacgctagga tcgagccatg caatcgagcc ggccgctggc
     3481 gcccgatgag ctggaccggc cgattcgcca cgcgatcccc gccctggccc gcggtgcgtc
     3541 cgcgctgtcc aagctcggca tcacgactcc ccgcgaggcc ctctggtacc tcccgttccg
     3601 ctacgacgac ttcagcgacc tgcggaccct ggatgccctc gtgccggacg agaagcagtc
     3661 cgcgctggtc cgcgtggacg ccgtccgtgt cgagccaggc ttcggccgcc gcccgcagcg
     3721 cgtcatcgcc cagctcagcg acgagcgcgg ttcggccgag gcgatctggt tcggccgccg
     3781 ctacgtcgag cggcggctgc gcgtcggcga cgaggtcgtc gtcagcggca aggtcagcca
     3841 gcgcggctgg cgcttccagt tcacctcgcc ggagttcagc cccgcgggcc acgagtcggt
     3901 gcacactgcc cgcgtcgttc cggtctacca cctggccgcc ggcgtcacgc agaagcggct
     3961 gcgcgaggtg ctcgcccgca tcctggacgt cgccctgccg gccgtcgagg acccgctgac
     4021 cgatgcggag cgcaacggtc tgccgcggct cgacgtggcg ctccagacgg cccacttccc
     4081 ggaagagacc gccgacgtca ccgatgcgct cgaccgcctc gccttcgacg agctgtacgc
     4141 gctgcagctc acgctggcgc aggcgcgccg cgcccgcgag cacctggtgg cgccgaagat
     4201 cgcggtcacg catgccgagc tggccgaggt cgtcgccgcg ctgccgttcg agctgacccg
     4261 cgatcaggcg agcgccgtgg aggcgatcgc gcacgacctg gcctcggagc ggccgatgcg
     4321 acgcctgctc cagggcgacg tcggcagcgg caagaccgcg gtggcggcgg tggcgctggc
     4381 cagcgcggtt cgcgccgggt ggcaggccgc cctcatggcg ccgaccgaga tcctggcccg
     4441 ccagcaccac cacggccttg cgccgctgct cgacgcgctc ggcgtccggg ccgagttcct
     4501 gtcgggttcg ctgacgccgg cgcgcaagcg cgagatccac gacgccatcg ccgggggcat
     4561 ggccgaggtg gtcatcggca cccatgccgt gatcagcgag gcggtcgagt tcccgagact
     4621 gggcctggcc atcgtcgacg agcagcaccg cttcggtgtc gggcagcgtg ccgcccttca
     4681 ggccaagggg agcgggggag agccgcacct gctggccctc accgccacgc cgatcccgcg
     4741 gacgctggcc ctgacggtgt acggcgacct cgccatctcg accatccgcg agctcccccc
     4801 gggccgccag cccatccgga cggagatccg cgaccggcag gcgctgccga agatcgaggc
     4861 gttcctcgcc tcggaagctg ccgaggggcg gcagtcgttc gtcgtcgtgc cgctggtgac
     4921 cgagtccgag tcgctccagg cggccagcgc cgaggccgag gccgaacggc ttcaggcggc
     4981 gctgcccgag ctgcgcgtcg ggctggtgca cggccagcag cgggccgacg cgcgggacgc
     5041 caccatgacc gccttcgccg ccggcgacat ggacctcctc gtcgccacca ccgtcatcga
     5101 ggtcggcatc gacgtcccga acgcgagcgt gatgctcatc gaggacgccg agcgcttcgg
     5161 cctggcacag cttcaccagc tccgcgggcg cgtcggccgc gggccgcacc gttcgttctg
     5221 catcctgctg tccgacgtga ccgagccgct ggcccgggag cggctcgagg tggtgcgtgg
     5281 ctcgcaggac gggttcgcca tcgcggaggc ggacctgcgg ctgcgcggcg ccggcaacct
     5341 gctcgggacg cggcagtcgg gcctgccgcc actgcggatc gcgtcgttgt tcgagccccg
     5401 acatctccgt ttggccgagc gcgcgcgtga gctggcggac acggtggtcg gtgccgatcc
     5461 gacgctggaa cgacgccccg ggctcgcgcg cctccaggcc gacttcaccc ggctggagga
     5521 ggagggggac gccgcctgat gcgcgtcatc gccgggaccg cccgtggggt tccgctgcac
     5581 gctccccggg gcgacgccac gcggccgatc accgaccgcg tcaaggagac gctgttcgcc
     5641 atcctcggcg atcgtgtgcc gggcgcccgg gtgctcgacc tgtacgccgg aaccggcgcc
     5701 atcggcatcg aggcgctgtc acggggtgcg gcaagcgcag acttcgtgga gcgggaccgg
     5761 catgccgtca cggcgctccg gtccaacctc gatcggacgc ggctgtccgc gtccgcatcg
     5821 gttcatgcca ccgacgtcga gcgcttcctg gggtcgtgtg gcaagtcgac gtgggacctg
     5881 gcgaccgtgg atccgccgta cgagacgcgt gatatcgtgg cgccgctccg cacgctggtg
     5941 ccccatctgg ctcccgacgc gatcgtggtg gtcaagcact tctggcgcac cgaggtgccg
     6001 gtggtggacc ggctcgtggc cgtgcgccag cggcgcttcg gcgagacgat gctgaccttc
     6061 ctgcagttgg aggctccatg acccgggtcg cggtcttccc cggctccttc gatcccatga
     6121 cgaacgccca cctcgacgtc gcgcggcgtg cgtcgggcct gtacgacgcg ctcgtcatcg
     6181 gcgtgctgaa caacccgaag aagcagccgc tgttcgacat ccacgagcgg gtcgacctca
     6241 tccgggcatg cgtggccgac ctcggcgccc acgtcagcgt ggacgccttc gacggcctga
     6301 ccgtcgactt cgctcggcgg cacggggcgc agttcatcgt ccgtgggctg cgcgcggtga
     6361 gcgacttcga ggccgagctg cagatggccc tgaccaatcg caagctcgcg cccgaggtcg
     6421 acacgacgtt cctgatgacg gccctcgagt acggctacgt gagcagctcg ctgaccaagg
     6481 aggtcgcgcg cttcggcggg gacatcacgc ccatggtgcc ggcgcccgtc gccgaggcgc
     6541 tggcgcggcg cctggggggc ggggcgcgtg gtgagccgcg cgtataatcg gccgccaagg
     6601 agggtcgcgc cacggacatc atcttcctcg tcgagcgcct ggagtcgctc gtcgcggcca
     6661 gcaagaagct gccgctgacg aacaacgtgg tgctggacca ggcggccgtg ctcgagctga
     6721 tcgatcagct gcgcgtctcc gtgcccgagg aggtccgcca ggcgcgccgc gtgaccgagg
     6781 aggccacgcg catcacggag cgtgcccgtg acgagggcga cgcgatcgtc gcccgcgcac
     6841 aggagcaggc agcccagatg ctcgaggagc gcgagctggt ccgcgccgcc cagcagcgcg
     6901 cggccgagat catcgaggcc gcccaggcgg aggcccggga gctgcggcgc ggcgcggacg
     6961 agtacgccgc gggcgtgctg atccgcctcg agggcgagtg catcaaggcg ctgacgagca
     7021 tcaagcgggg gatcgacatg ctcgacgagc gccatcgggt ggctcccgcc ggcgatggac
     7081 gacagccggc ggcgaacgac gccatcgtgg tcgacgccga ggacgaggct cccgccacct
     7141 cgcgaccatg accgccttca acgtcgccgg gctgctccac gagccgcccg ggtcgacgcg
     7201 ggacatccgc ctgcgcgacc actacgtcac cctgggtccc gacgtcgagc tggccggccc
     7261 actcgacgcc gatctgcgcc tgcagcgcac caatcgcggc atcctggtgc gcggcgagct
     7321 gcgagcgccg ctgcggcggg tctgcggccg atgcaccgag ccgtacgtgg acgacgtccg
     7381 cgtggcggtc gaggaggagt tcctgccgac cctggatccg gtgaccggcg tgccggtgcg
     7441 caccgatggc tccgaggacg agacgaccca gtggatcgac gagcatcacg agatcgagct
     7501 cgacggtgtc ttccacgacg agctggccct gacggagccg atgctcccgc tctgccgtcc
     7561 cgactgcccc ggcctgtgcg tggagtgcgg tgagcgcctc gaccgtggcg atcacgccca
     7621 cggcggaccc gagatcgatc cacggctcgc cgggctggcg gccctcctga accgtggcga
     7681 ccctggcgag gacgacccgg ggcgcaactg atcgacgccg gggcgcgcat ttcgcatccg
     7741 gcctcgcgcc gggtatactg cggcgtcccc gtcttcatcg gtcaaggagt ttccgcgtgg
     7801 gcgtccccaa gcgaaaggtt tccaaggcgc gccagggcga gcgccgcgca cacctcgcca
     7861 tcagcgcgcc gccgctcgtc gagtgcgagc actgtcacga gctgcatcgg gcccatcacg
     7921 tctgcccgac gtgcggctgg tacaacggcc gcgaggccgt tgcgatcgag ccggtcaccc
     7981 cgaccgaagg tcagcgctaa ctgagcatgc gcatcgcggt cgacgcgatg ggaggcgacc
     8041 acgcccctcg cgagatcgtc cgcggagcca tcgactacgc cacggcctcg cccgaccagg
     8101 tgatcctggt cggtgatgtg ccgcgtctgg agcgcgagat cgcggcgtac ggcaaggggc
     8161 gtccgggcaa cctcgatctc gtcgacgccc ccgaggtgat cgagatgggg gagcacccgg
     8221 ccacggcgct gcgctcgaag cgcaaggcct cgatcgtggt cgccaccgac ctggtgcgcg
     8281 acggcgaggc cgatgccgtc gtctcggcgg gttccaccgg cgccaccatg gccgccgcgg
     8341 ttttccggct gggccgcatc gagggcatcg accgtccggc gctgcccgcg cacatgctga
     8401 ccgccacggg cccggtgatg ctgctggacg tcggcgccaa cgtcgactcc gacccggaga
     8461 acctggtcca gttcgcggcc atgggcgcca tcttcgccga gcacgtgctg cacgtgcgca
     8521 acccgcggat cggcctgctc aacatcgggg aggagccgga gaagggcgac gagctggcgc
     8581 ggcacgccca cgagaagctg agcaccctcg acctgcactt cgtcggcaac gtcgaggctc
     8641 acgacatgat cgcgcaccgt gccgacgtgg tggtctgcga cggattcgtc ggcaacgtgg
     8701 tgatcaagtt cttcgagggc atcaccagct tcatcttccg cgcgctgcgc gaggacctgc
     8761 agcagggacc gatcgcgccg ctggccctgc tggcactgaa gccgggcttc gacaagatga
     8821 agacccgctt cgactacgag cgctacggcg gcgcgccgct gctcggcgtg aagggcgtga
     8881 gcatcgtgac ccacgggcgc gcgaaggcac ggatgatcga gaacgccatc cgggtcggat
     8941 cggaggcggc gcacgcgcgc gttccctcgc tcatcgagca gtggacccgc gagcacccgg
     9001 ccctcgccca ccgcggcgtc cgctcgaggc tggcggcgcg gctccaccgg gagcgcgcgt
     9061 gagcccggcc gcctcgccgt cacgcgcgca tcaccgcgga ggccccggca tggtcgcacg
     9121 acggctggcg ctgctggcgc tcttcgagga cgagttccgg cctggcagcg ccgtgcaggc
     9181 gctggatcgg ctggcctcgg agcgggacgt cgccgacgac gtccgcgctc acgctcgcgc
     9241 catcgtggat ggcgtcgtgg aacatcggtc cgacctggac gcccggatcg ctgcggaggc
     9301 cccgctgatc cccgttccgg agctcggtcg ggtggaacga accatcctcc ggagcgcgct
     9361 gtacgaggtg ctatactccg ccgcgacccc gagcggggag ccgatgcacg atgcggtgac
     9421 cctcgcccgg atctacgccg gcgatgccgc gcgacggctc gtcaacggcg tgctagggag
     9481 cgtttccaga tcgggtggag gagcctgaat tcgtgactga cgggaccacg tacgaacgac
     9541 tcaagaagat catcgtcgag cagctcggcg tcgacgagga agaggtcacg cctgaggcct
     9601 cgttcgtcga ggacctgaac gccgactcgc tggacctggt cgagctca
//
LOCUS       DASTYP010000021         6988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_146744, whole genome shotgun sequence.
ACCESSION   DASTYP010000021 DASTYP010000000
VERSION     DASTYP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146744"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VFV59_01040"
     CDS             complement(<1..1818)
                     /locus_tag="VFV59_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU5324451.1"
                     /translation="MPPIDPTELLNPVNAGYVADLYEQYRSDPTSVDPDWRALFDSGV
                     AGFEPVASPAAEPSGNGQASAPEAGAAPPAPPAAEPMQPTPPAQPAPAGAPAAASVPD
                     GATAIKGPSARLVQNMVASLAVPTATSFREIEVATLERRRKELNGQIAPKKVSFTHLI
                     GWAIVQAASDQRSMSHYFTEADGQAYRVDPGAINLGLAVDVERSSGRFLVVPVIKGAD
                     DMDFAGFHARYEELVEKARTNKLSPDDFVGATITLTNPGTLGTTASVPRLMPNQGTIV
                     ATGTIRDVGPARRMTITSTYDHRIIQGAESGLFLQRVEQLLGGADGFYEDVFGAMGAR
                     EPSEPEPEPEPEPQPEPPSPSSEAATAVSPAEAIGAPPRGATNGEGFERLKAVAAGMA
                     LVKAYRHFGHLAAHLDPLGAEPLGDPALDPVPLGLTPELMSEVPAELLRIYVPGETLA
                     EALPHLQATYSGTMAYEVEHIGSHEERVWLRQVIESGEHGRPMQAEDKVALLERLISV
                     EALERFLHRAYLGQKRFSIEGLDSMVPMLDVVIQDAADQGARKVMIGMAHRGRLNVLA
                     HVVGVSYQAILAEFEAGRAGAEAATGPKGGSDDVKYHLGA"
     gene            1905..2213
                     /locus_tag="VFV59_01045"
     CDS             1905..2213
                     /locus_tag="VFV59_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5324452.1"
                     /translation="MIIRAWRGRVRSDRADAYVDYLRRTGLPDLARTPGNRGVTLLRR
                     PVDGDLVEFDVRSHWESLDDIRAFAGEEVDRARYYPEDADFLLEMSERVEHWEVVDER
                     "
     gene            complement(2227..2874)
                     /locus_tag="VFV59_01050"
     CDS             complement(2227..2874)
                     /locus_tag="VFV59_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324453.1"
                     /translation="MTETRYPEPAPIDPPPAEQPNAAAPPLTPPVAEPARAEGTNPAI
                     GLGAILVVVGLLLFLGQVLDFGLGDMGWPFWVIAAGVAILLVGLMIANERGVVVAGTI
                     VTTVGLVLLYQNSTGHWESWAYAWALVGPAASGLGLAIWGIRSGSAGDVRAGTWGLLG
                     GLAFFAILFLFFEGMIGLSGERLPIDEWVLPALVIVIGGVVLARGLLSRGEPETT"
     gene            2881..3672
                     /locus_tag="VFV59_01055"
     CDS             2881..3672
                     /locus_tag="VFV59_01055"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEU5324454.1"
                     /translation="MLRASRKGHTYHGDVDVNADLGESYGRWTLGDDVALVRHLTSAN
                     LACGFHAGDFSVMERTVALCRDAGVAVGAQPGYPDLLGFGRRPMPFSPEEVESLVRYQ
                     IGALDAFCRAAGVELRHVKPHGALYNQAAADPLLAGAIARAVARFSRELLLFGLASSE
                     PMRSAAADAGLRFVPEAFADRRYLADGSLQPRSQPGSLITDATAAAAQAVRVAVDGEV
                     RAVDGTAVTIDAESICCHGDTPGAVEIAGAVRAALATAGVEVSAP"
     gene            3672..4325
                     /gene="pxpB"
                     /locus_tag="VFV59_01060"
     CDS             3672..4325
                     /gene="pxpB"
                     /locus_tag="VFV59_01060"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEU5324455.1"
                     /translation="MRPFGEAALLVEVADARVAQSVARTLGQLPPISGVVPGRASVLV
                     EFDPATSTEGEVRSLLERALVRRSEGEVAGRQRTIPVVYGGERGPDLEEVAGHLGMTA
                     DEVVAHHATVELEVLFCGFASGFAYLGDLPAELRVPRLATPRTRTPSGSVAIADGMTG
                     IYPADLPGGWPVIGWTPIRLFDADRVPPTYLLPGDRVRFRAVSGADAAGFAGRPEDW"
     gene            4322..5266
                     /locus_tag="VFV59_01065"
     CDS             4322..5266
                     /locus_tag="VFV59_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEU5324456.1"
                     /translation="MSIEILDPGALTLVQDAHGRPGWRHVGVPVGGAADAWSARLANR
                     LVGNDDDAALLEMTLHGATLRFDAPALVALTGGLDARLGGFPVRRGVSVRVHAGATLS
                     VGGGDGLRGYLAVAGGIDVPEVLGSRGTDLRTGFGGHEGRALRAGDRLTVGAADVVGR
                     RRWRAAREAGPIRILPGPHADAVPVAALEDAEWVVGSEADRTGVRLDGSRMTVGVREV
                     PSMGLPLGAIQVPGDGRPIVMLADRPVTGGYAVPAVVIGADVGRVAQLRPGDGLTFGS
                     VSLQEALEADRRAAEELAAIELLGEPDDDELSWVGSLD"
     gene            complement(5286..5603)
                     /locus_tag="VFV59_01070"
     CDS             complement(5286..5603)
                     /locus_tag="VFV59_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324457.1"
                     /translation="MLEITSGAAALLTEIRREQSVPEDHGLRVFPEMTEPGEVAIGLG
                     FTESPAPSDQVTEKDGLRVFVAQELATPLQDAAIDVTQEDGADRLVFRPQGGDSQQPP
                     MQA"
     gene            complement(5784..6647)
                     /locus_tag="VFV59_01075"
     CDS             complement(5784..6647)
                     /locus_tag="VFV59_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5324458.1"
                     /translation="MAGRLMLRAHRQTRDKALARAVISPTYHLQGCRTHNPRVSGWYA
                     AICTLHGMAIDRRRPVDVGALRATELRRWVGRDLRIMRVTGGSPQRRVAARSGISQPF
                     LSVVERGGGGASIEVLSTLAEAVGGRLSMKVIPGDGVSLRDSGQLEIVQLIARLRHPR
                     WRHAIEQPVADPPDRRAADLVLELATEVNMIEVERWWVDHQAQLRALQLKRDALSHRM
                     GRRANLVICVLDTRRNRAALHRFEASVQESFPIRARAVWHALQSGQPIGGDAFVWIRP
                     YALRRAADSSG"
     gene            complement(6680..>6988)
                     /locus_tag="VFV59_01080"
     CDS             complement(6680..>6988)
                     /locus_tag="VFV59_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5324459.1"
                     /translation="PMPIAPTLADGVAGNIERTSITWQLCRRLVDEVVVVDEEAIRDA
                     LRWSLEVPHVLLEGSAVLGVAALRSRAVDVTDRRVAVVATGRNVGFDTVREVLASRPG
                     "
BASE COUNT          937 a         2455 c         2543 g         1053 t
ORIGIN      
        1 cgcgcccagg tggtacttca cgtcgtcgga gccgcccttc ggccccgtgg ccgcctccgc
       61 cccggcgcgt ccggcctcga actcggccag gatcgcctgg tagctgacgc cgaccacgtg
      121 ggccagcacg ttgagccggc cgcggtgcgc catgccgatc atcaccttgc gcgcgccctg
      181 atcggccgcg tcctggatga ccacgtcgag catcgggacc atcgagtcga ggccctcgat
      241 gctgaaccgc ttctgcccca ggtaggcccg atgcaggaag cgctcgagcg cttccacgct
      301 gatcagccgc tccaagagcg cgaccttgtc ctcggcctgc atcgggcgcc catgctcgcc
      361 ggactcgatc acctggcgca gccacacgcg ctcctcgtga gagccgatgt gctcgacctc
      421 gtacgccatg gtgccgctgt acgtggcctg cagatggggg agcgcctcgg ccagggtctc
      481 gccggggacg tagatgcgca gcagctccgc cggcacctcg gacatgagct cgggggtgag
      541 gcccagcggg acgggatcca gcgccgggtc cccgagaggc tcggcgccca gggggtccag
      601 gtgcgcggcc aggtggccga agtgccggta cgccttgacc agtgccatcc cggcggccac
      661 cgccttgagc cgttcgaacc cctcgccgtt ggtcgcgcct cggggcggcg caccgatggc
      721 ctcggccggg ctgaccgcgg tggcggcctc agacgacggc gacggtggtt ccggttgggg
      781 ttcgggttcc ggttcaggct ccggttcgga gggctcgcgc gcccccatgg cgccgaacac
      841 gtcctcgtag aagccatcgg ccccgcccag cagctgctcg acgcgctgca ggaagagccc
      901 cgattcggcg ccctggatga tgcggtggtc gtaggtcgag gtgatcgtca tccggcgcgc
      961 cggcccgacg tcgcgaatgg tcccggtggc aacgatcgtt ccctggttcg gcatcagccg
     1021 cgggacgctc gcggtcgtgc ccagggtgcc ggggttggtc aaggtgatcg tggcgccgac
     1081 gaagtcgtcc ggggacagct tgttggtccg cgccttctcg acgagctcct cgtagcgggc
     1141 gtggaagccg gcgaaatcca tgtcgtcggc gcccttgatg accggcacga ccaggaagcg
     1201 gccgctggat cgctcgacgt ccacggcgag acccaggttg atggcgccgg ggtcgacgcg
     1261 gtacgcctgc ccatcggcct cggtgaagta gtggctcatg gagcgctggt cgctggcggc
     1321 ctgcacgatc gcccagccga tcaggtgggt gaagctgacc ttcttcggcg cgatctgtcc
     1381 gttcagctcc ttccggcgcc gctccagcgt ggcgacctcg atctctcgga agctggtcgc
     1441 cgtcgggacg gccaggctgg ccaccatgtt ctggaccaat cgtgcggacg gacccttgat
     1501 ggcggtcgcg ccgtcaggga ccgatgcggc cgccggcgcg cctgccgggg ccggctgcgc
     1561 cggtggcgtg ggctgcatcg gttcggcggc gggcggtgcc ggaggtgccg cacctgcctc
     1621 aggtgccgat gcctgcccgt tgccggaagg ctcggccgcg gggctggcca ctggctcgaa
     1681 tccggccacg cccgagtcga agagcgcgcg ccagtcggga tcgacgctcg tcggatcgga
     1741 tcggtactgc tcgtagaggt cggcgacgta cccggcgttg accgggttca gcagctcggt
     1801 gggatcgatg ggcggcatgt ccgggtaagg atacggtcct cgccgctgcc tcgcgacgag
     1861 cgcgggtacc ctcgacgggt caccgaagag gaggttcgca tccgatgatc attcgagcct
     1921 ggcggggtcg cgtccgctcc gaccgcgccg atgcgtacgt cgactacctg cgccgcaccg
     1981 gcctgcccga cctggcgaga acgccgggca accgcggcgt cacccttctg cgccggccgg
     2041 tggacggcga tctcgtcgag ttcgacgtcc gatcccactg ggagtcgctc gatgacatcc
     2101 gcgccttcgc gggtgaggag gtcgaccggg cgcgctacta ccccgaggac gccgacttcc
     2161 tgctcgagat gtccgagcgc gtcgagcact gggaggtggt ggacgagcgc tgaggagtcg
     2221 cgggcctcag gtcgtctcgg gctcgccccg tgacaggagg ccgcgcgcca ggaccacccc
     2281 accgatcacg atcaccagcg ccggcaggac ccattcgtcg atcggcagcc gctcgccgct
     2341 gaggccgatc atgccctcga agaagaggaa caggatcgcg aagaaggcga gcccgccgag
     2401 caggccccac gtccccgcgc gcacgtcacc ggcgctgccc gacctgatgc cccagatggc
     2461 caggccgagc ccgctggccg cgggtccgac cagggcccac gcataggccc acgactccca
     2521 gtggccggtc gagttctggt acagcagcac caggccgacg gtggtgacga tcgtccccgc
     2581 gacgacgacg ccgcgctcgt tcgcgatcat caggccgacc agcaggatcg cgacgccggc
     2641 cgcgatgacc cagaacggcc agcccatgtc gcccaggccg aagtcgagca cctgccccag
     2701 gaagaggagg aggccgacga ccaccaggat cgcccccagc ccgatggccg ggttcgtccc
     2761 ttcggctctc gctggctcgg cgacgggagg ggtcagtggc ggcgcggcgg cgttcggctg
     2821 ctcggctgga ggagggtcga tcggcgccgg ctcgggatag cgcgtctcag tcatgagccg
     2881 atgctacgcg cgagccgcaa gggccacacg taccatggcg acgtggacgt gaacgccgac
     2941 ctcggcgaaa gctacggccg ctggacgctc ggcgacgacg tcgcgctggt ccggcacctc
     3001 acgagcgcca acctggcctg tggcttccac gccggcgact tcagcgtcat ggagcggacc
     3061 gtcgcgctgt gccgcgatgc cggcgtcgcc gtcggggctc agccgggcta tccggacctc
     3121 ctcggcttcg gccgccggcc gatgcctttc agtccggagg aggtcgaatc gctggtccgg
     3181 taccagatcg gcgcgctgga cgccttctgc cgggcggccg gcgtcgagct gcgccacgtc
     3241 aagccgcacg gcgccctcta caaccaggcc gccgcggatc ctcttctggc gggcgcaatc
     3301 gcgcgcgccg tcgcgcgctt cagcagggag ctgctgctgt tcggcctcgc ctcctcggag
     3361 ccgatgcgct cggccgccgc cgatgccggc ctccgcttcg tccccgaggc cttcgcggac
     3421 cgccgctacc tggccgatgg ctccctgcag ccgcgctcgc aacccggctc gctcatcacc
     3481 gatgcgaccg ccgcggccgc ccaggccgtg cgcgtggcgg ttgacggcga ggttcgcgcg
     3541 gtcgacggga ccgccgtgac gatcgacgcg gaaagcatct gctgtcatgg cgacacgccg
     3601 ggcgccgtcg agatcgccgg cgcggtgcgg gcggcactcg ccactgccgg cgtcgaggta
     3661 tcggcaccct gatccgtccg ttcggcgagg cggcgctgct cgtcgaggtc gccgatgccc
     3721 gcgtggcgca gtccgttgct cgcacgctcg ggcaattgcc gcccatcagc ggcgtcgtgc
     3781 ccggacgcgc atcggtgctg gtcgagttcg acccggccac ctccaccgag ggcgaggttc
     3841 ggtcgctcct cgagcgcgca ctggtgcgtc ggtctgaggg agaggtcgcc ggtcggcagc
     3901 ggacgattcc cgtcgtgtac ggcggcgagc gcgggcctga cctcgaggag gtggcgggcc
     3961 accttgggat gacggctgac gaggtggtgg cgcaccacgc aaccgtcgag ctggaggtgc
     4021 tcttctgcgg cttcgcctcc ggcttcgcgt acctgggcga cctgccggcg gagctccgcg
     4081 ttccgcgtct cgcgacgccg cgaactcgga cgccgtccgg gtcggtggcc atcgcggacg
     4141 gcatgaccgg catctacccg gctgatctgc ccggcggctg gcccgtgatc ggctggacgc
     4201 cgatccgcct gttcgacgcc gaccgcgttc cgcccaccta cctgctcccc ggtgaccggg
     4261 tgcgcttccg cgccgtatcc ggagccgatg cggccggctt cgccggtcgg ccggaggact
     4321 ggtgagcatc gagatcctgg acccgggcgc cctgacgttg gtccaggacg ctcacggtcg
     4381 tccgggttgg cggcacgtgg gtgtgccggt cggtggggcg gccgatgcgt ggagcgcgcg
     4441 cctggccaac cgactggtcg gcaacgacga cgatgccgcg ctgctcgaga tgacgctcca
     4501 cggggcgacg ctgcggttcg atgccccggc gctcgttgcg ctcaccggtg gcctggacgc
     4561 acgactcggc ggcttcccgg tgcggcgcgg ggtgagcgtc cgggtgcacg ccggcgcgac
     4621 gctgtcggtc ggaggaggcg acggattgcg gggctacctg gcggtggccg gcgggatcga
     4681 cgtgcccgag gtgctcggct cgcgcggcac cgacctgcgg accggcttcg gcggccacga
     4741 gggccgtgcc ctgcgtgccg gggaccggct caccgtcggg gctgccgacg tggtcgggcg
     4801 ccgccgctgg cgcgccgcgc gcgaggccgg ccccatccgg atcctgcccg gcccgcatgc
     4861 cgatgccgtg cccgtggcgg cgttggagga cgcggagtgg gtcgtggggt cggaggcgga
     4921 ccggacgggt gtccgcctgg acggttcgcg catgacggtc ggcgtgcgcg aggtgccgtc
     4981 catggggctg ccgctcggcg ccatccaggt cccgggcgac ggacggccca tcgtcatgct
     5041 ggcggatcgt cccgtgaccg gcggctacgc ggtcccggcg gtcgtcatcg gtgccgacgt
     5101 cggtcgcgtc gcccagctgc ggccgggcga tgggttgacg ttcggatcgg tcagcctgca
     5161 ggaggctctc gaggcggacc ggcgggcagc ggaagagctc gccgccatcg agctcctggg
     5221 ggagccggac gacgacgagc tctcgtgggt cggctcgctc gactgagcga gcctggggat
     5281 cgcggctagg cctgcatcgg cggctgctgg gagtcgccgc cctgcggacg gaagacgagg
     5341 cggtccgcac cgtcctcctg ggtgacgtcg atggccgcgt cctgcagcgg cgtcgcgagc
     5401 tcctgcgcca cgaagacgcg cagcccgtcc ttctcggtga cctggtcgct gggcgccggg
     5461 ctctcggtga agccgaggcc gatcgcgacc tcgccgggct cggtcatctc ggggaagacg
     5521 cgaagtccgt gatcctcggg aaccgactgc tcgcgtcgga tctcggtcag cagcgcagcg
     5581 gcgccgctcg tgatctcgag cattgggtga atctccttgg gtttggtacc cgggggcggc
     5641 ccgcaagacc gataccacgg ctgttgcgat cgcagcgccg atgcatggaa ctgatcacag
     5701 tgcgcatgac tgcgccgtac ggcgttgcct gatgcgtcgg gttgatcggg cggcgcgcca
     5761 ggcaggcgtg cggtgcactg tgatcagccg gagctatctg cggcgcggcg cagggcatat
     5821 ggccgaatcc aaacgaaggc gtcgcccccg atgggctgtc ccgattgcag cgcgtgccac
     5881 acggcacggg cgcggatcgg gaagctttcc tgcacggagg cctcgaagcg gtgcaacgcg
     5941 gcccggttcc gccgcgtgtc gagcacacag atcaccaggt tcgcgcgtcg gcccatgcgg
     6001 tggctgagcg catcgcgctt gagctgaaga gcacgcagct gggcctggtg gtccacccac
     6061 caccgctcga cttcgatcat gttgacctcg gtcgccagct cgagcacgag atcggctgcc
     6121 cggcggtccg gggggtcggc aacgggctgt tcgatcgcat gcctccagcg cggatgacgc
     6181 aggcgagcga tgagctgaac gatctcgagc tggccggagt cgcgcagcga aacgccatca
     6241 cccgggatga ccttcatgct cagtcgaccg ccgaccgcct cggcgagcgt cgacagcacc
     6301 tcgatgctcg cgccgcctcc gccgcgttcc acgacggaca ggaacggctg ggatatgccg
     6361 ctgcgtgcgg caacccgccg ctgcgggctc ccgcccgtca cgcgcatgat ccgaaggtcg
     6421 cgcccgaccc aacggcgcag ctctgtcgca cgcagggcac ccacgtccac ggggcggcgc
     6481 cgatcgattg ccatgccgtg aagtgtgcaa atcgccgcgt accagccgct tacccgtggg
     6541 ttatgggttc ggcacccttg gaggtgatag gtgggactga tcacagcgcg cgccaaggct
     6601 ttgtcgcgcg tctgccgatg cgcccgaagc atcaggcgac ccgccatagt cgccccacgt
     6661 gcactgtgat cagtaggagt tatccgggcc ggctcgcgag cacctctcgc accgtgtcga
     6721 atccgacgtt gcgacccgtg gccaccaccg ccacgcgacg atcggtgaca tccacggcgc
     6781 gggagcggag ggcagccacg cccaagacgg cgctcccctc gagcagcaca tgcggcacct
     6841 ccagggacca gcggagagcg tcccgaatgg cctcctcgtc caccaccacc acctcgtcga
     6901 ccaggcgccg gcacagctgc caggtgatgc tcgtgcgctc gatgttgccg gccacgccat
     6961 cggcaagggt gggggcgatg ggcatcgg
//
LOCUS       DASTYP010000022         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1312481, whole genome shotgun sequence.
ACCESSION   DASTYP010000022 DASTYP010000000
VERSION     DASTYP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312481"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VFV59_01085"
     CDS             complement(<1..1125)
                     /locus_tag="VFV59_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEU5324460.1"
                     /translation="MLGAAAIRLAGLNRPTELVFDEIFYARDACWYVAGSQAVCGITE
                     LTSRSHPPLGKWLIGAGIAAFGYEPFGWRITAALAGTATVALTYLLAWRLLRGWVAGA
                     ALTVGAAAASGLLATDLLHLVQSRVAMLDVFIALFVVGAVLAVVLDRDRDRAKDDVGW
                     RRLALGRPWRLLAGACLGAATATKWSGAYVAPAVIGLVVAWEIADRRRRDPDAGWGAW
                     IGRALRAEALPTVVLLGIVPVLVYVASYTGRMPGELLAVPWDPTSVWNGIWQHQRAML
                     DFHTGLGGDHPYQSPPWSWIALRRPVAYWFSDEGGSYREILALGNPAVWWPAILALIG
                     LVWTWWRSGWQLLRPEPVILAAAVSTYLPWLVLSGGRSQTF"
     gene            complement(1288..1734)
                     /locus_tag="VFV59_01090"
     CDS             complement(1288..1734)
                     /locus_tag="VFV59_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324461.1"
                     /translation="MTDRLRRHRRLLVAPIALLGALAVALTAPMPAVPDDGLTTVRAE
                     VADRLPGWRLDRLEPSWEGGYTVVTSCAGLELDFQLVPGHGLPAEDAWVQPSDAYARS
                     RLRAVSDHWRYLVWYRDPVRPRSLSCSEELARLGTGDLPLVERRFD"
     gene            1917..2357
                     /locus_tag="VFV59_01095"
     CDS             1917..2357
                     /locus_tag="VFV59_01095"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU5324462.1"
                     /translation="MNYELYMAEAIAEARRGAEEGEEPVGAVAVLDDAMIARDHDRSV
                     THGDPTAHAVMLTLQATARKLGTRRLAEVTIFTTHEPCAMCVGAMVESRVRSLVFAMP
                     DPDRGAAGSVIQLARDPALPHQLSVISGVRESEARRLDTESASV"
     gene            2422..2508
                     /locus_tag="VFV59_01100"
     tRNA            2422..2508
                     /locus_tag="VFV59_01100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2456..2458,aa:Ser,seq:cga)
     gene            2520..2607
                     /locus_tag="VFV59_01105"
     tRNA            2520..2607
                     /locus_tag="VFV59_01105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2554..2556,aa:Ser,seq:gga)
     gene            2651..2916
                     /gene="ffs"
                     /locus_tag="VFV59_01110"
     ncRNA           2651..2916
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFV59_01110"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          460 a         1050 c         1052 g          372 t
ORIGIN      
        1 gaacgtctgg ctgcggccgc cggacaggac gagccacggc aggtaggtgg acaccgccgc
       61 cgccaggatc accggctcgg gccgcagcag ctgccagccc gatcgccacc aggtccacac
      121 caggccgatc agcgccagga tcgccggcca ccacacggcc gggttcccga gcgccaggat
      181 ctcgcgatac gagccgcctt cgtcactgaa ccagtacgca acgggccgcc tgagggcgat
      241 ccacgaccac ggcggcgact ggtacgggtg gtcgcctccc aggccggtgt ggaagtcaag
      301 catcgcccgc tggtgctgcc agatgccatt ccagaccgat gtcgggtccc acggcaccgc
      361 gagcagctcg cccggcatcc gcccggtgta gctcgccacg tacaccagca ccggcacgat
      421 cccgagcagc acgacggtcg ggagggcctc cgccctcagc gcccgcccga tccacgcgcc
      481 ccagcccgcg tccgggtcgc gccgtcgccg atcggcgatc tcccacgcca cgacgaggcc
      541 gatgacggcc ggcgccacgt acgcgcccga ccacttcgtc gcggtcgccg cccccaggca
      601 cgccccggcg agcaggcgcc agggccgccc gagcgccagc cgtcgccacc ccacgtcgtc
      661 cttcgcccga tcgcggtccc ggtcgagcac gacggcgagg acggcgccga cgacgaacag
      721 ggcgatgaag acgtccagca tggcgacgcg cgactggacg aggtgcagga ggtcggtcgc
      781 cagcagcccg gaggcggccg cggcgccgac ggtcagggcg gcgcccgcca cccagccgcg
      841 caggaggcgc catgcgagga ggtaggtcag ggcgacggtc gcggttcccg ccagcgccgc
      901 ggtgatgcgc cacccgaagg gctcgtagcc gaaggcggcg atcccggcac cgatgagcca
      961 cttgccgagt ggcggatggg aacgactggt gagctccgtg atcccgcaca ccgcctggct
     1021 gccggcgacg taccagcagg cgtcgcgcgc gtagaagatc tcgtcgaaga cgagctcggt
     1081 tggccgattc aggcccgcga gccggatggc ggcggcgccg agcacgatga cgaggaaggc
     1141 ggcggtgtcg atccacgtcc agcgcggcgc cggacgttcc tcggctgggt cgatggcacg
     1201 atcccctcgg acggacggcg cgcgccggca cccggcaccg cggcgtccga gcgcatccta
     1261 gtcaaggtcg gcggcggatc gccagcctca gtcgaagcgg cgctccacca gcgggaggtc
     1321 gcccgtgccc aggcgcgcca gctcctcgct gcacgagagg ctgcgcggcc gtacgggatc
     1381 gcgataccac accaggtagc gccagtggtc ggagacggcc ctcaggcgag agcgggcgta
     1441 ggcatcgctg ggctggaccc atgcatcctc ggccggcagc ccgtgcccgg gaacgagctg
     1501 gaagtcgagc tccagcccgg cgcagctggt gacgacggtg tagccgccct cccacgacgg
     1561 ctccaggcgg tcgagccgcc acccgggaag gcgatcggcg acttccgcgc gcacggtggt
     1621 gagaccgtcg tcggggacgg ccggcatcgg cgcggtcagc gccacggcga gtgcgccgag
     1681 caacgcgatc ggcgcgacga ggaggcgtcg gtgacgacgc agtcgatcgg tcatgggtgg
     1741 tgggaggatg tggtcctgtg gctggtggag atcggcgtcg aggcgcgagc cacgacgcgg
     1801 tttgatggtc gaacgcttcc cgacgagtgc aggttccgta ccggggaccg gatgctagac
     1861 tcggtcgcag gcccgccact ccaccagccg gcgggaggcg cagcagacgg accgacatga
     1921 actacgagct gtacatggcc gaagcgatcg ccgaggcgcg gcgcggcgcg gaggagggcg
     1981 aggagccggt cggggccgtg gcggtcctcg acgacgccat gatcgcacgg gatcacgatc
     2041 ggtcggtgac gcacggcgac ccgacggcgc acgccgtgat gctgaccctc caggccaccg
     2101 cccgcaagct cggcacgcgc cggctcgcgg aggtcacgat cttcacgacc cacgagccgt
     2161 gcgccatgtg cgtcggcgcc atggtggaga gtcgggtccg gtcgctcgtg ttcgccatgc
     2221 ccgatccgga ccgcggcgcg gctggcagcg tcatccagct ggcacgcgac cccgcgctgc
     2281 cgcaccagct gtcggtcatc tccggcgtgc gtgagtccga ggcacgccgg ctcgacaccg
     2341 agtccgcatc ggtctagtcg tcgttatctc ctggccgcgt tttggccgat gcgaccgtct
     2401 gcgctagact cgctgcggcg cggagaggtg tccgagtggt ttaaggtgcc gctctcgaaa
     2461 agcggtgtgc gatgagcacc gtgggttcga atcccaccct ctccgccact cctcctctcg
     2521 gagaggtcgc ctagaggcct agggcgcggc actggaaatg ccgttcgggg caacccgacg
     2581 tgggttcgaa tcccaccctc tccgccatcc cgtttgaccg cccaccgcgg cggccctacg
     2641 atccccgcgg tcgtgctagg cggggaacta gcggtgccct gtatccgcaa tccgctacag
     2701 cggagctgaa ctcccggtcg aggtccgcgc cgctcgccca ccaccaccgt ggtccgacgt
     2761 cgagagtcgg gtcccacgca gcgtcggtcc gtgaaccccg tcaggtccgg aaggaagcag
     2821 cggtaagcga tcgccggcgg gtgccgtggg acaacctggc tggagccggg cccgtgggtg
     2881 agtgcgagtc gacgcgaatc gacaccgggt gcacgaccga tttcctcccc tcaa
//
LOCUS       DASTYP010000023         9880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1203543, whole genome shotgun sequence.
ACCESSION   DASTYP010000023 DASTYP010000000
VERSION     DASTYP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203543"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..3346
                     /locus_tag="VFV59_01115"
     CDS             20..3346
                     /locus_tag="VFV59_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324463.1"
                     /translation="MLASETANYISLDFVASAPHTYDHSTGGGAFDDRTVGVDDDIVE
                     SLEGGDFACGDTVTFLTEISLDGSQDDPNVARTIQLEYSFLLDTTGQTGLALGPITYV
                     GINYGNVGGDGAGGTDAGMNDDLGSTATLISQSAPAVVKPPYFTAGATNDVVIDVTDL
                     EDSETVILRIDAEINCLFNSSPTGNLQADLQSATVIAIGGQEAADTVPGGAQTVPFKA
                     AGDVVFPGKIIVDKVTDPAGATQSFNFTVTAPGSANADPYSDAFSLTDASTPHNSGEI
                     DPSTAQIGNQTVTIAGNYTVSEINIPSAWQLDTLVCTSDDNGQVYSGTAASTTFSLHE
                     NEIVTCVFTNELQQGTLKVVKYHDLNADGDRDAGEGPLSGWEFFIDNNADGVWNAGDS
                     AKQSTNASGEATFTLNAGTYSVCEVLQATWFNSDPGTATPCESATVVAGQETTKSFGN
                     YQNGTKSGFKYEDEAADGDYDAGADALLEGWNIYAIRDDNGDGQLDAAELSDWTLDET
                     DAVTGAYSFSLSPGKYFICEETGGSAWSQSSPASGQTGFTGECDDQNATANPDLAPGG
                     YYITVTSQSTDTGNEFGNFRQGTKSGTKYHDLNADGDRNAGDVALGGWVIRAYVDDNG
                     DGILQATETTIADSATTDATTGAYELQLDPGDYVVCEVSQAGWTQSAPANTLCDLGAG
                     LEPGGWAITVTSNSTDVGNDFGNFQEVDQTVLKYHDLNADGDRDAGEPVLSGWTFFID
                     ENANEVLDAGEVSALTNASGIATFEDLMPGASYDICEVLTSGWLNSDPSGATLCESTD
                     TIVSGSTPTQLVFGNFQEVDQTVLKYRDLNNSAVRDPGEPVLSGWTFFIDVDGDEILD
                     AGEVSVITGASGTATFNDLMPGASYSICEVLTAGWLNSEPVGSTLCRATGTLVSGVTP
                     GTLEFGNRLPSLVIDKVASTETITISGPNDALVATPSVVTWTLTYTLTEGPVTNAVIT
                     DPVPAGFTFLDASNGGTFANGVVTWNLGTLMSSGSVTFRTTVDPTTISRIAPTVNTAT
                     IDSNETPPDQGQDSVTVSVVPPPLAGNPTPKPSLPNTAAGIGMDGQPVTVPVELLVAF
                     FLGSLGVLTLANVRARSRRQ"
     gene            complement(3506..4360)
                     /locus_tag="VFV59_01120"
     CDS             complement(3506..4360)
                     /locus_tag="VFV59_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324464.1"
                     /translation="MSAARKLAPMTSEERIARAIAAIRTDLEPDPLFQRRLRSEVLGR
                     YVAAREGAVVPERGRPAKEMGRLGRAVLYASFALGLSVTGVMAASQQAVPGDLLYPLK
                     RDIEALRHRVLPAEFEELLARVELKARLDELAAVAERGDAAAVVLLADEVHQSYGAVI
                     EQTADSPQNLVVLDALLERLPERARAAVSDVVLDVDRATHPAPSRPVPGQGRRGESPA
                     YGRDDGSGKGPAAAPPTDASDSTGANPVPTASPEPTESSAEATPPAAAPSADADAQAE
                     NQGADADQ"
     gene            complement(4357..5088)
                     /locus_tag="VFV59_01125"
     CDS             complement(4357..5088)
                     /locus_tag="VFV59_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5324465.1"
                     /translation="MRRRTAAPRLALFADARLWYNYALNRASELPVPNRSLGAVLRTT
                     IVEQEVVTRPSPTVPGALADDATIDELARAATSGDVEAVGRLYDALVGPIHRYVAMRL
                     RRREDAEDVTQLVFERIVAALPRYRHSGRPFAAWAFRIARNAVIDHQRRQRPTEPLGR
                     IAEPADQFEIDARSLQEEELRELRAAIRSLTPDQQEALALRYAAGLSAEEAARVMGRQ
                     AGTVRGLTFRAIEALRRLLVGGRSS"
     gene            complement(5100..6314)
                     /locus_tag="VFV59_01130"
     CDS             complement(5100..6314)
                     /locus_tag="VFV59_01130"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU5324466.1"
                     /translation="MTDTSARTRNPLAFLDAEIDELKAKGLYRRLRVVQSEQKSRCVI
                     DGREVITLSSNNYLGLNTHPRLRQAALDAVERFGAGSGAVRTIAGTMSLHEELEARLA
                     TFKHVEAVLTFQSGFTCNTGVIPILAPEGAVIVSDALNHASIIDGIRLTKAERRIFPH
                     ADMEALRSTLRDVRAADGGADKTILVLTDGVFSMDGDIAKLPEIVEAAEEADAIVYVD
                     DAHSSGVLGRNGRGSVDHFDLHGRVHVQVGTLSKAIGVLGGYVAGSASLRTVLEHKAR
                     PFLFSTSHPPAVAAACIAAIDVLEQEPELIDRLWENARHFKGGLERLGFDTGHSETPI
                     TPVIVGAGALAHRFSDRLFEEGVFAMGIGFPTVPEAKSRVRTIVTAEHTRDELDTCLE
                     VFEKVGRELAII"
     gene            complement(6562..7617)
                     /gene="tdh"
                     /locus_tag="VFV59_01135"
     CDS             complement(6562..7617)
                     /gene="tdh"
                     /locus_tag="VFV59_01135"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU5324467.1"
                     /translation="MSGSAGDPSTMRALAKLTPGPGLELVDRARPTPGPGEVLLRMEA
                     TSICGTDYHLFRWDEWAAENVVPPRILGHELAGTVIGTGAGVSRVREGDLVGVESHVV
                     DWTCAQCRRGQMHLCRNLRVIGVHVDGGFAEEMVIPEANAIESNGLDPAVVALQEPMG
                     NAVHAAFAEPIEGKSVLVTGCGPIGLCSVGIARAAGASLVVATDTEGYRLELARTMGA
                     DLALDARDPETEQRIADATGGDGVDVVLEMSGAAPALDQALRVITRGGSISLLGIFGE
                     PPTVDLSELVIQKGVRLHGIYGRRIYDTWERTQSLLRSGALDVSPILTHRFDLADWRD
                     AFELIRTRHAGKVVLLP"
     gene            complement(7614..8525)
                     /locus_tag="VFV59_01140"
     CDS             complement(7614..8525)
                     /locus_tag="VFV59_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5324468.1"
                     /translation="MKLLAYGRPGSDRSGTRLGLQTDAGIRDLTATLGASDVGEMLRH
                     GIGLDELRDRAVHAELVTGDVVPRAPIARPGKIICVGLNYHDHCREQSIEPPTYPMLF
                     SKFANAVADPGAAVTRPRATEQLDLECELGVVIGRRASRIGRDEAGGVIFGYTIVNDV
                     TMRDLQKEDRQWLRAKGSDGFAPMGPVVVSADELGDPGSVRLRSSVNGETWQDSSTAE
                     MVFDVRTIVAFASRTITLEPGDVIATGTPAGVGHDQSPPRYLADGDVMRCEIEGIGVI
                     ENRVVDETPRSSDHRAAAGVDELFVPG"
     gene            complement(8551..8976)
                     /locus_tag="VFV59_01145"
     CDS             complement(8551..8976)
                     /locus_tag="VFV59_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324469.1"
                     /translation="MTDDAPQPTAPMPLGVRAWFVWAFGLLVFTGLFLGRIVEFIDFS
                     ERAPFSLLGIFMMVELAVLLFGITVALQRKRIAHRFAIVIALIGIPVLAGLPPILLDF
                     PQDATNGWWGVVGVASAIVAIALVVALLRPVARAWFSEP"
     gene            9116..>9880
                     /locus_tag="VFV59_01150"
     CDS             9116..>9880
                     /locus_tag="VFV59_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5324470.1"
                     /translation="MESRVARPRIDDVARHAGVSKTAVSFAFNQPENLNAATRDRILA
                     VASELGYRPSPIARRLAARRTDQLGLVVPQPTHDTFANPFLAELVRGIGDACDAEGFS
                     IVIVPPFHGSIARAVDAALVDGLLLLGLAPTHPDLAVARGTGIAVVGLDIDHRAGGPL
                     DGLDVITVDDEQGAEEAAAHLLGLGHRTFAVVLMAEHPMSSLDERSGISGRRLRGIRR
                     GLGLADDGDASPDGSIRLHLLSAPVTFEGGQQAFDAI"
BASE COUNT         1588 a         3509 c         3335 g         1448 t
ORIGIN      
        1 gccgcttgtc caggagcagg tgctcgcgag cgagaccgcc aactacatct cgctcgactt
       61 cgtggcgtcc gcaccgcaca cctacgacca cagcaccggc ggtggcgcgt tcgacgaccg
      121 caccgtgggc gtcgacgacg acatcgtgga gtcgctggaa ggcggcgact tcgcctgtgg
      181 cgataccgtc acattcctca ccgagatctc cctcgacggg tctcaggacg acccgaatgt
      241 cgctcgcacg atccagctgg agtattcctt cctgctcgat acaaccggcc agaccgggct
      301 cgctctcggt ccgatcacgt acgtcggtat caactacggc aacgttggcg gcgacggggc
      361 gggcggcacg gatgccggga tgaacgacga ccttggaagc acggcgactc tgatctccca
      421 gagcgctccg gcggtcgtca agccgcccta cttcaccgcg ggcgcgacga acgacgtcgt
      481 catcgacgta accgacctcg aagactccga gacggtcatc ctccgcatcg atgcggaaat
      541 caactgcctg ttcaacagca gcccgaccgg aaacctgcag gctgaccttc agagtgcaac
      601 ggtgatcgcc atcggcgggc aggaggctgc ggacacggtt ccgggcggcg cgcagaccgt
      661 gccgttcaag gccgccggcg atgtcgtctt ccccggcaag atcatcgtcg acaaggtgac
      721 cgatccagcc ggtgccaccc agtccttcaa cttcaccgtc acggcgcctg ggtcagcgaa
      781 cgccgatccc tacagcgacg cgttctcact taccgatgcc tcgacaccgc ataacagcgg
      841 agagattgac ccaagcaccg cgcaaatcgg caatcagacg gtgacgatcg cgggcaacta
      901 caccgtctcc gagatcaaca tcccaagcgc ctggcaactc gacacgctgg tgtgcaccag
      961 cgatgacaac ggccaggtct actcggggac ggccgcatcg acgaccttct cgctgcatga
     1021 gaacgagatc gtcacctgtg tgttcaccaa cgagcttcag cagggaacgc tgaaggtcgt
     1081 gaagtaccac gacctgaacg ccgacggcga ccgtgacgcc ggcgagggac cgctctccgg
     1141 ctgggagttc ttcatcgaca acaatgccga tggagtttgg aacgcgggcg actccgcgaa
     1201 gcagtcgacc aatgcaagcg gtgaggccac cttcaccctg aacgccggca cgtactcggt
     1261 gtgtgaagtt cttcaggcca cctggttcaa ctccgacccc ggtaccgcga cgccatgcga
     1321 aagcgcgaca gtcgtcgccg gccaggagac gaccaagtca tttggcaact accagaacgg
     1381 caccaagagc ggattcaagt acgaggacga ggcagccgac ggcgattacg acgccggggc
     1441 cgacgcactt ctcgagggct ggaacatcta tgccattcgt gatgacaacg gtgacggcca
     1501 gctggacgct gccgagctct cagactggac gctggacgag acggatgccg tcacgggcgc
     1561 ctatagcttc agcctgagcc cgggtaagta cttcatctgc gaggagaccg gcggaagcgc
     1621 ctggtcacag tcctcgcctg cgtcggggca aaccggcttc acgggcgagt gcgacgacca
     1681 gaatgcgact gccaatccgg acctcgcgcc cggcgggtac tacatcaccg tcaccagcca
     1741 atcgaccgac acaggcaacg agttcggcaa cttccggcag ggcaccaagt ccggcacgaa
     1801 gtaccacgac ctcaacgccg acggcgaccg taacgcgggc gacgtggcgc tcggcggatg
     1861 ggtcatccgc gcctacgtcg atgacaacgg cgacggcatc ctgcaagcca ccgagaccac
     1921 catcgccgac tcggccacca ccgacgccac taccggcgcc tacgagctcc agctcgaccc
     1981 cggcgactac gtcgtgtgcg aggtcagcca ggccggctgg actcagtcgg cgccggctaa
     2041 cacgctgtgt gatcttggcg caggcctcga gccgggcggc tgggccatca ccgtcaccag
     2101 caacagcact gacgtcggaa acgacttcgg caacttccag gaggtcgacc agaccgtcct
     2161 gaagtaccac gacctcaacg ctgacggcga ccgtgacgcg ggcgagcccg tgctctcggg
     2221 ctggaccttc ttcatcgacg agaacgccaa cgaggtgctc gacgccggtg aggtgtccgc
     2281 gctgaccaac gcctccggca tcgcgacctt cgaggacctg atgcccggcg ccagctacga
     2341 catctgcgag gtcctgacca gcggctggct gaactccgat ccgtccggcg ccacgctgtg
     2401 cgagtcgacc gacacgatcg tgtcgggcag cacgcccacg cagctggtgt tcggcaactt
     2461 ccaggaggtc gaccagaccg tcctgaagta ccgagatctg aacaacagcg cggtccggga
     2521 tcccggtgag ccggtgttgt caggctggac gttcttcatt gacgtcgatg gcgacgagat
     2581 ccttgacgcc ggcgaagtca gcgtcatcac cggcgcgtcc ggtacggcga ccttcaacga
     2641 cctgatgccc ggcgccagct actcgatctg cgaggtcctg acggctggct ggctcaactc
     2701 cgagccggtt gggagcactc tgtgcagggc gaccgggacg ttggtctccg gcgtcacgcc
     2761 tgggacgctc gagttcggca accgcttgcc gagcctcgtg atcgacaagg tggcgagtac
     2821 cgaaacgatc acgatcagcg ggcctaacga cgctctcgtc gcgaccccgt ccgtcgtaac
     2881 gtggacgctg acctacacgc tcacggaggg tccggtcacc aacgcggtga tcaccgaccc
     2941 ggtccctgcg ggcttcacgt tcctcgacgc gtcgaacggc ggcacgttcg ccaacggcgt
     3001 tgtcacctgg aacctcggca cgctgatgtc gagcggaagc gtcacgttcc gcacgaccgt
     3061 ggatcccacg acgatctcgc gaatcgcgcc gaccgtgaac acggcgacga tcgattcgaa
     3121 cgagacgccg ccggaccagg gtcaggacag cgtgaccgtc tcggtcgtgc ctccgccgct
     3181 ggcgggcaat ccgacgccga agccgtcact gccgaacacg gcagccggga tcggcatgga
     3241 cggccagccg gtgacggtgc cggtcgagct cctggtggcc ttcttcctcg gttcgctcgg
     3301 cgtcctgacg ctggcgaacg tgagggcgag gagccgccgc cagtaggccg ttagccgcgc
     3361 ggggccggac gcaccgaccg gtcccgcgca attcatccag cccccctggc agggacggga
     3421 tcgaggttcg cctcggtccc gtcttctggt tcgagcgagg aactcctgcg ggaggcgagc
     3481 gcccggcgcg aggtctccgg gcggactact ggtcggcgtc cgcgccctgg ttctctgcct
     3541 gggcatccgc gtcggcggac ggcgcggcgg ccggcggcgt tgcctccgcg gacgactccg
     3601 tgggctcggg ggaggccgtc ggcaccgggt tcgcccccgt ggagtcggag gcatcggtgg
     3661 gcggagctgc ggccggaccc ttgcccgatc cgtcgtcacg gccgtaggcc gggctctcgc
     3721 cacgccggcc ctggccaggc acggggcgac tcggggcggg atgggtcgcg cggtcgacgt
     3781 ccagcacgac gtcggagacc gcggcgcgtg cccgctcggg gaggcgctcg agcagcgcat
     3841 cgaggacgac gaggttctgc ggcgaatcgg ctgtctgctc gatcaccgct ccgtacgact
     3901 ggtgcacctc gtccgcgagg aggaccacgg ccgcggcgtc tccccgttcg gcgaccgccg
     3961 cgagctcgtc caggcgcgcc ttcagctcga cccgcgcgag cagctcctcg aactccgccg
     4021 gcaggacgcg gtggcgcagc gcctcgatgt cgcgcttgag cggatagagg aggtcgcccg
     4081 gaacggcctg ctgcgaggcc gccatcacgc ccgtcacgct gagacccagg gcaaagctcg
     4141 cgtacagtac cgcccgtccc aggcgtccca tctccttcgc gggacgtcca cgctcgggga
     4201 cgaccgcgcc ctcgcgagcc gcgacgtacc ggccgagcac ctcgctgcga agacgccgct
     4261 ggaagagcgg atccggctcc aggtcggtgc ggatggcggc gatggcgcgc gcgatgcgct
     4321 cctccgacgt catcggcgcc agcttgcggg ccgcgctcac gacgaccgcc ctccgacgag
     4381 caggcgccgc agcgcctcga tcgcacggaa cgtcagtccc cggacggtgc cggcctgccg
     4441 tcccatgaca cgcgccgcct cctccgcgga gaggcccgcc gcatagcgca gggccagggc
     4501 ctcctgttgg tccggggtca gcgaacggat cgccgcccgc agctcacgga gctcctcctc
     4561 ctggagcgaa cgcgcgtcga tctcgaactg gtccgccggc tcggcgatcc ggccgagtgg
     4621 ctccgtcggc cgctgccgtc gctggtggtc gatgacggca ttgcgcgcaa tgcgaaaggc
     4681 ccacgccgcg aagggtcggc cgctgtggcg gtaccggggc agggcggcca cgatccgctc
     4741 gaacaccagc tgcgtgacgt cctccgcgtc ctcgcggcgg cggaggcgca tggcgacgta
     4801 gcggtggatc ggtccgacca gcgcgtcgta caggcgaccg accgcctcga cgtcaccgct
     4861 cgtggcggct cgcgccagct cgtcgatggt cgcgtcatcg gccagcgcac cgggaaccgt
     4921 cggcgacggg cgggtgacga cctcctgctc gacgatggtg gtgcggagga cggcgccgag
     4981 agaacggttg ggcacgggca gctcggatgc tcggttgagg gcgtagttgt accagaggcg
     5041 cgcatcggcg aacagggcca aacgaggggc ggcggtgcgc cgccgcatcg gtccagccgt
     5101 cagatgatgg ccagctcccg cccgaccttc tcgaagacct ccaggcaggt gtccagctcg
     5161 tcgcgggtgt gctcggcggt gacgatggtc cggacccggc tctttgcctc cggcacggtc
     5221 gggaagccga tgcccatggc gaacaccccc tcctcgaaca gccgatcact gaaccgatgc
     5281 gccagggcgc cagcacccac gatcaccggc gtgatcggcg tctccgagtg gccggtatcg
     5341 aagccgagtc gctccagccc gcccttgaag tggcgcgcgt tctcccacag ccgatcgatc
     5401 agctccggct cctgctccag cacgtcgatg gcggcgatgc acgcggccgc gacggcgggc
     5461 ggatgcgagg tggagaagag gaacggccgg gccttgtgct ccaggacggt gcgcagcgac
     5521 gcggatcccg cgacgtagcc acccagcacg ccgatggcct tcgacaacgt cccgacctgg
     5581 acgtggacgc ggccgtggag gtcgaagtgg tcgacgcttc cccggccgtt ccggcccagg
     5641 acgccggacg agtgcgcgtc gtcgacgtag acgatcgcgt ccgcctcctc cgccgcctcg
     5701 acgatctccg ggagcttcgc gatgtcaccg tccatgctga agacgccatc ggtcaggacc
     5761 aggatggtct tgtcggcgcc accatcggcc gcgcgaacgt cgcgcagcgt ggaccggagc
     5821 gcctccatgt cggcgtgcgg gaagatccgc cgctcggcct tcgtcagccg gatgccgtcg
     5881 atgatgctcg cgtggttcag cgcgtcgctg acgatcaccg ccccctccgg ggccaggatc
     5941 gggatgacgc cggtgttgca ggtgaagccg gactggaagg tgagcaccgc ctccacgtgc
     6001 ttgaacgtcg ccagccgcgc ctcgagctcc tcgtgaaggc tcatggtgcc ggcgatcgtc
     6061 cgcaccgccc cgctgccggc gccgaagcgc tcgacggcgt ccagtgccgc ctgccgcagc
     6121 cgcgggtgcg tgttcagccc gaggtagttg ttcgagctga gggtgatgac ctcccggccg
     6181 tcgatcacgc agcggctctt ctgctcgctc tggacgaccc tgagccgccg atacaggccc
     6241 ttcgccttca gctcgtcgat ctcggcgtcc aggaaggcga gtgggttgcg cgtgcgtgcg
     6301 ctcgtatcgg tcatctccgt caggataccg cggctctggc cgcggcttcc gggacacgcc
     6361 ggcgcgtcgc gatggatcgc gtgacctgca cccgaggagc tcgacgtggt ccgatgtcgc
     6421 gatcaatcgc ggagaggtga gctgcggagg ccgcgtcccg gtgcgctcac gtcggcgaac
     6481 tctcgatcca tcgcgatcgc ggcgcacatc cgccgcctgc ggcgagatcg tggcgattga
     6541 tcgcgacgac agcggcctcc ctcagggcag caggaccacc ttcccggcgt ggcgggtgcg
     6601 gatgagctcg aaggcgtcgc gccaatcggc caggtcgaac cgatgcgtca ggatcggtga
     6661 gacgtcgagg gcccccgacc gcagcaacga ctgcgtccgc tcccacgtgt cgtagatgcg
     6721 gcggccgtag atgccgtgga gccggacgcc cttctggatc acgagctcgg agaggtcgac
     6781 ggtcggcggc tcgccgaaga tgccgagaag cgagatggag ccgccgcggg tgatcacgcg
     6841 cagcgcctgg tcgagcgccg gggcggcgcc gctcatctcg agcaccacgt ccacgccgtc
     6901 gccgccggtc gcatccgcaa tccgctgctc cgtctcgggg tcgcgcgcgt ccagggccag
     6961 gtccgccccc atggtccgcg ccagctccag gcggtagccc tcggtgtcgg tggcaacgac
     7021 cagcgacgcc ccggcggcgc gcgcgatccc gaccgagcac agcccgatcg gcccgcagcc
     7081 ggtcacgagc acggacttcc cctcgatggg ctcggcgaag gcggcgtgga ccgcgttgcc
     7141 catcggctcc tggagcgcaa cgacggccgg gtcgagcccg ttcgactcga tggcgttcgc
     7201 ctcggggatg accatctcct ccgcgaagcc accgtccacg tgcacgccga tgacgcgcag
     7261 gttgcggcac aggtgcatct gaccgcgccg gcattgggcg caggtccagt cgaccacgtg
     7321 cgactcgacg ccgacgaggt ctccctcgcg aacccgcgac acgcccgcgc cggtcccgat
     7381 caccgtcccc gccagctcgt ggcccaggat gcgcggcggc accacgttct cggcggccca
     7441 ctcgtcccag cggaagaggt ggtagtcggt gccgcagatg ctggttgcct ccattcgcag
     7501 cagcacctca ccggggcctg gcgtgggacg ggcccggtcc accagctcga gccctggccc
     7561 gggggtcagc ttggccaggg cgcgcatggt gctcgggtcg ccggcgctcc cgctcatccg
     7621 ggcacgaaca gctcgtcgac gcccgccgcc gcccggtggt ccgaggagcg cggcgtctcg
     7681 tccaccaccc ggttctcgat gacgccgatc ccctcgatct cgcagcgcat gacgtcccca
     7741 tccgccaggt agcgcggcgg cgactggtcg tgcccgaccc cggccggcgt gccggtggcg
     7801 atgacgtcgc ccggctcgag ggtgatggtg cgcgacgcga acgccacgat ggttcgcacg
     7861 tcgaacacca tctcggctgt cgacgagtcc tgccaggtct ccccattgac cgaggagcga
     7921 agccgcaccg acccggggtc gcccagctca tcggcgctga cgacgaccgg gcccatcggc
     7981 gcgaagccgt ccgagccctt ggcgcgcagc cactgccgat cctccttctg gaggtcgcgc
     8041 atggtgacgt cgttgacgat ggtgtagccg aagatcacgc cacccgcctc gtcacgaccg
     8101 atgcgtgagg cgcgtcggcc gatcacgacc cccagctcgc actcgagatc cagctgctcg
     8161 gtggcccgcg gcctggtgac ggctgcaccg gggtccgcca cggcgttggc gaacttgctg
     8221 aacagcatcg ggtaggtcgg cggctcgatg gactgctcgc gacagtggtc gtggtaattg
     8281 agcccgacgc agatgatctt gccgggccgc gcgatcggcg cgcgcggcac gacgtcgccg
     8341 gtcaccagct cggcgtgaac cgcccgatcg cgcagctcgt ccagtccgat gccgtggcgc
     8401 agcatctccc ccacatcgct ggcgcccagc gtcgccgtga ggtcgcggat gcccgcatcg
     8461 gtttgcagcc ccagccgggt gccggatcgg tccgatccgg gtcgcccgta ggcgagcagc
     8521 ttcacgcggc cagtatagga atcgggctgg tcacggctcc gagaaccagg cgcgtgccac
     8581 cgggcgcagc agggccacca cgagcgcgat ggcgacgatg gcgctcgcca ccccgaccac
     8641 gccccaccag ccgttggtcg cgtcctgcgg gaagtcgagc aggatgggcg gcagcccggc
     8701 gagcaccggg atcccgatca gggcgatgac gattgcgaac cgatgcgcga tgcgcttgcg
     8761 ctgcagcgcc acggtgatgc cgaacagcag caccgccaac tcgaccatca tgaagatgcc
     8821 gagcaggctg aacggtgcgc gctcgctgaa gtcgatgaac tcgacgatcc gtcccaggaa
     8881 cagcccggtg aagaccagca gcccgaaggc ccagacgaac caggcgcgaa ccccgagggg
     8941 catgggcgcg gtgggctgcg gcgcgtcgtc agtcatcggc gcgcggatgg tagcaagaag
     9001 cgccgaccgg ctcttgacac cgttcggcgg tccccggtac agtccggctc ctaaaccggt
     9061 tcactaaacc ggttcatcga cagggcgatg gcggtggcgc cctgccctgg agcggatgga
     9121 gtcgcgggtg gcccgacctc ggatcgatga cgtcgcacgg catgccggcg tgagcaagac
     9181 ggcggtcagc ttcgcgttca accagcccga gaatctcaat gccgcgacgc gcgaccgaat
     9241 cctggccgtg gcctcggagc tcgggtaccg accctcaccc atcgcccgcc ggctggcggc
     9301 gcgccgcacc gaccagctcg ggctggtcgt gccgcagccg acccacgaca ccttcgccaa
     9361 cccgttcctc gccgagctgg tgcgaggcat cggcgacgcc tgcgacgcgg aaggcttctc
     9421 catcgtcatc gtgccgccgt tccacgggtc gatcgcacgc gccgtggacg cggcgctggt
     9481 cgacgggcta ctgctgctgg gcctcgcccc cacccacccg gatctggcgg tggcccgcgg
     9541 caccggcatc gcggtcgtcg gcctggacat cgaccatcgc gccggcgggc cgctggacgg
     9601 cctcgacgtg atcacggtcg acgacgagca gggcgccgag gaggccgccg ctcatctgct
     9661 gggactcggg caccgtacgt tcgccgtggt cctcatggcc gagcacccca tgtcctcgct
     9721 cgacgagcgc agcggcatct ccggccgccg gctgcgcggc attcgccgcg gcctgggcct
     9781 cgccgatgac ggtgacgcgt caccggacgg cagcattcgc ctccatctcc tgtccgctcc
     9841 cgtgaccttc gagggcgggc agcaggcctt cgacgccatc
//
LOCUS       DASTYP010000024        16464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1203576, whole genome shotgun sequence.
ACCESSION   DASTYP010000024 DASTYP010000000
VERSION     DASTYP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16464
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203576"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFV59_01155"
     CDS             <1..690
                     /locus_tag="VFV59_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5324471.1"
                     /translation="RVTDLVSAEQVRARHDRLLRRLREVADAHGHDPGRLRIVGVTKE
                     HPLEVVAAAVDAGLTRLGESRVQEAEPKSAAFPAIEWHFIGRLQSNKARRAVRSFPVI
                     HSIDSPELLQRVDRIADEEGRRPQVLLEVNVSGEATKAGLRPEDLDEISAPAVAELVG
                     LMTIARFGASEEEARAVFARLRALRDRLQQRLGRELPELSMGMSADAEAAAAEGATYV
                     RIGTALFGPRH"
     gene            710..961
                     /locus_tag="VFV59_01160"
     CDS             710..961
                     /locus_tag="VFV59_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5324472.1"
                     /translation="MTAVLIRFIELLAFVVWFLLIARVVMSWTNPRGGGGLTAFVYQA
                     TEPILAPIRRVLPPSGGVDWSPLIAMLILGVVVRLVAQL"
     gene            973..1245
                     /locus_tag="VFV59_01165"
     CDS             973..1245
                     /locus_tag="VFV59_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEU5324473.1"
                     /translation="MAELQVIVTPRAARDAIEAVHDGALRVRVTRPPAGGEANRSVIR
                     LVATALDVPPSSISIASGQRARHKRLVLASLDEAELRRRLAAIGHD"
     gene            1295..1371
                     /locus_tag="VFV59_01170"
     tRNA            1295..1371
                     /locus_tag="VFV59_01170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1329..1331,aa:Val,seq:cac)
     gene            1396..2130
                     /locus_tag="VFV59_01175"
     CDS             1396..2130
                     /locus_tag="VFV59_01175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEU5324474.1"
                     /translation="MADRAPDAAPRGNRVPASEVRSMFDRIAPIYDAMNTVMTAGLDA
                     RWRRAAAAAAGLGPGMRVLDVACGSGALAREVARAVGPTGEVVGIDLSERMLERARHR
                     PPDAAAAPIRYEVGDALALPEPDATYDATTIAFGLRNVADYAGCVREMRRVVRPGGRV
                     VVLELATPRGGIGRLITRTWFERVVPIVGRLAGGGSAYGYLPSSVRGYPPPEEVAALL
                     RDAGLVDVTWRRLRPGLVTLHVGRRP"
     gene            2127..3320
                     /locus_tag="VFV59_01180"
     CDS             2127..3320
                     /locus_tag="VFV59_01180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5324475.1"
                     /translation="MTGLRVLLTTESYLPHQSGVTVAVDALARGLKARGHDVVVLAPR
                     PARGATLPAVGSPGPAPRYAWLESYQLPAVAPLGYRMPWPLPWSAGLEVARAHRPQVV
                     HAHSPFVTGLMARRLARAFEAPLVFTHHTRYGDYAHYAGPVARLAGWGVDEYLERFWR
                     ACTAVIAPSTDLAAEIRSSAPSLRIEVIPTGVDVAGLGAVDAVDPRPLAGWPDDAVVA
                     ASLGRLAPEKSPHTLLEALAIAVPDVPRLRLLLIGGGPSEADLRRRAAAPDLAGKIAF
                     TGTLPRPEALARLAGADLFVFASQTETQGLVLAEALSVGLPAVALEGPGVRDSVRDGI
                     DGRVVRADDPHQRAAELAQAMASLAADDTARAASAERARAGADRFSIERRIEETEALY
                     RSVLR"
     gene            3384..3650
                     /locus_tag="VFV59_01185"
     CDS             3384..3650
                     /locus_tag="VFV59_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324476.1"
                     /translation="MRVSLVARFVSHPSIDFGSGRRSALRPRLGLSRERTVYHRARRR
                     ESTATIGALRTHLSGFRFPAVGRASHRPARRPAHPFGLPRARRA"
     gene            3659..4117
                     /locus_tag="VFV59_01190"
     CDS             3659..4117
                     /locus_tag="VFV59_01190"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5324477.1"
                     /translation="MPILRSARATSAGGVVYRRTPEAGLEVVLVHRRSPPLWALPKGT
                     PDSGETLEETALRETREESGLEVQLEQPIRAIRYFFVRGTTRFHKTVHFYLMRVTGGS
                     PDDHDAEFDEVRWMPVTEALALLTHATERSVVERAVEMLEAGTGGQEATA"
     gene            4120..5226
                     /gene="gcvT"
                     /locus_tag="VFV59_01195"
     CDS             4120..5226
                     /gene="gcvT"
                     /locus_tag="VFV59_01195"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU5324478.1"
                     /translation="MGELRRTPLDGVHRELGARMVPFAGYEMPVQYTSILEEHRTVRS
                     AVGVFDLSHMGELEVRGEEAMAFLRYALVSDPGTLDIGQAQYSMLCDADGGIIDDLIC
                     YRLGEDRYLVVCNASNRFAVVEQLGSLLERGDFGAELEDRSDRTGLVAPQGPNAAALL
                     AELTDLDLTAIANYHAAEGTVAGIRCLVARTGYTGEDGFELFCPASDTVALWRAVLQA
                     GERHGAKTCGLGARDTLRLEAGMPLYGNELGRDVNPYEVNLGRVVKLEKGEFVGRAAL
                     QAIQQAGPSRKLIGLQMVDNAIPRHGYEVRAGGRAVGSVTSGTLSPTLGTKIAMALVE
                     ADTAGLGNAFEVVVRDRSYAAEQVKLPFYRRPRE"
     gene            5262..5639
                     /gene="gcvH"
                     /locus_tag="VFV59_01200"
     CDS             5262..5639
                     /gene="gcvH"
                     /locus_tag="VFV59_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU5324479.1"
                     /translation="MSYPDDLRYSKEHEWVRVEGTRATIGITSFAADELGDIVFVELP
                     EVGATLSQFATFGVVESVKAVSDLFAPVSGEVLEVNEALRESPELLNSDAFGDGWIAT
                     LELSDPSEVDTLLDAQAYAAETA"
     gene            5653..6996
                     /gene="gcvPA"
                     /locus_tag="VFV59_01205"
     CDS             5653..6996
                     /gene="gcvPA"
                     /locus_tag="VFV59_01205"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEU5324480.1"
                     /translation="MTYSPHTPADRERMLAAIGVGSVDELFDDIPAAVRADPWSLPDP
                     LSEQEVRHELARLANRNRIPDVSFLGAGVYRHLVPAVVGEVIGRSEFATAYTPYQPEV
                     SQGTLQSIYEFQSLICELTGMEVATASHYDGATATAEAALMACRLTRRDRIAVSAGLN
                     PDYRRVLETYCSGPGIEVVTVPVDLAEGGTGTTDASAAEAALGDDVACLVAAQPNFYG
                     QLEPMAELGEAAHAAGALFVAVVEPTSLALLQAPGDYGADIVAAEGQPLGIPVSFGGP
                     YVGLMAARMSSVRQMPGRLVGATRDVDGRRGYVLTLQAREQHIRREKAASNICTNQAL
                     MALAATTYLTAIGPSGLREVAELSTTQARHLAAALEAAGVGRRRFDGPYFAEVTMQVP
                     DASRRHAALVERGIVAGYLPERDEPALRDTLLLAATELTTDEHIERLVSALVITR"
     gene            7071..8642
                     /gene="gcvPB"
                     /locus_tag="VFV59_01210"
     CDS             7071..8642
                     /gene="gcvPB"
                     /locus_tag="VFV59_01210"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEU5324481.1"
                     /translation="MTVVEPLIFESSTPGRRAVRFPSASERARELAGGQPELPASVRR
                     AAPPRLPEVSELDLLRHFNRLSHLNHAIDLGFYPLGSCTMKYNPKVNEWAARLPGLAE
                     SHPLDPEELAQGSLELMWLLSELLKEISGLDAVSLQPAAGAHGELTGMLMTRAFHRAN
                     GEGEQRTKVLIPDSAHGTNPATATMVGYQAVQIRSNERGGIDLDALRAALGSDVAALM
                     ITNPSTLGIFEDQIDQVVAAVKEAGAIAYMDGANLNAIMGRFRAGDAGFDVMHFNLHK
                     TFSTPHGGGGPGAGPVGVSERLAPFLPAPLPLLVDGDPAEVIANAWAGRSTPGARFAL
                     DNDRPQSIGKVRTAYGNFGMFVRAYTYIRSNGGSGLATISEDAVIAANYLRVRLADAY
                     DLPYDRICKHEVVFSGRRQKRDHGVSTLDIAKAILDHGIHPPTIYFPLIVEEALMIEP
                     TETESVETLDRFVDVMHEIAERAASDPASLKAAPTTTPVRRLDEATAARHPDLRYIFK
                     DTAESPRTVASEQTA"
     gene            8874..9464
                     /locus_tag="VFV59_01215"
     CDS             8874..9464
                     /locus_tag="VFV59_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5324482.1"
                     /translation="MEARQPDDLVERMQADDLDAFEEFFNTYKRPVYATAMAITRDPF
                     LAEEVLQDCFVKAYRVRERLDPARSPLPWLQRVAINLCYSRVSRRRAIAEPITSLITN
                     LVVDLAARPDQVVESREVLDVLQRGIDALPPKQQTAIILYYLHGYSLAEAAEIADCNV
                     GTMKSRLHYALKALRARLPVERRLPVGTPAAVRVTE"
     gene            9461..10180
                     /locus_tag="VFV59_01220"
     CDS             9461..10180
                     /locus_tag="VFV59_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324483.1"
                     /translation="MSCRRVCRELLWLARFGEFGPSSQPHLDHLAGCQHCRDEVGFDR
                     ALVQQLRVALAERVQDAAPSPDAFQAILARTRQPERRRWFVSWTTALGRLRFATAMAG
                     TGLAVVLALNMEVVRVDVPDTGPARGGEAVLQQVPRMPPGHTTLVALGQRGDGGPAAS
                     RPDPEAALMWAATIPLAPAPAGGQEEPAEEPAEAPSVELRVAFRPFQIHDPGAGASAP
                     DDPDDAGVVWAPVELSQGEPS"
     gene            10303..12033
                     /locus_tag="VFV59_01225"
     CDS             10303..12033
                     /locus_tag="VFV59_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5324484.1"
                     /translation="MRTWRLFSVFAVLVLVLAACGQQGGASASAGEESEAPPSSSEGA
                     EPSEGAAFEPSAYPEDGPAECGADNNPSNISEIRAEDERTVVFTLCSPDVAFLSKIAF
                     SSFAIDDSAYLEAHGADGSIVDQPNGTGPYRFDTWNRGSDITMTAFEDYWGDAPLSPT
                     VIFRWSAEAAQRLVELQSGTVDGIDNPAPDDFAAIEGDSNLEIFPREGLNVFYIGFNN
                     TYEPFNDEKVRQAIAMGIDRQRIVDNFYPPGSEVATHFTPCAISFGCEGEDWYEFDPE
                     GAKALLAEAGFPDGFDTQIHLRDVVRGYLPLPTAVAADIQAQLQENLGINAEIDVQES
                     TTFIDNADAGNLPGIHLLGWGADYPDPTNFLDYHFGGGASAQFGDKFDDITEALATGA
                     STVDETERAAAYETANNAIKQHVPMVPIAHGGSATVFQAGLEGAHSSPLSNEQMSVIA
                     PGDDDQLVWMQNGEPGGLYCADESDGEALRVCEQIKESLYAYETAGTAAIPALAEECT
                     PNDDLTVWTCTLREGVTFHDGSTLDANDVVLSYAVQWDADHPLHIGRDGSFTYFAALF
                     GGFLNPPPAE"
     gene            12125..13201
                     /locus_tag="VFV59_01230"
     CDS             12125..13201
                     /locus_tag="VFV59_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324485.1"
                     /translation="MLQFILRRLLLSIPVLFGIIFLVFALARLLPGDPCRAALGERAT
                     DAICNAFNARFGLNEPIPVQFVTYLGQLARGDLGDSIRQGRPVSEILLERLPVTLELT
                     LFALLFAVIVGVTLGRLSARRRNSPLDVGTMLVANLGVSIPVFVLGLLLAYLFAVVLR
                     GTPIALPPSGQLSPGVAHQSLQQAWGLGPVDGFLGALLTFVSNMVIFSSLITGQWTPF
                     LDGLKHIVLPMVALGTIPMAIIARMTRSSMLDVLGLDYIRTARAKGAPERLVMGRHAF
                     RNALLPVVTVVGLSLGALLSGAVLTESIFLLPGLGRTLFEAITGRDYIVVQALTLVTA
                     VSYVLINLVVDVSYAYLDPRIRLR"
     gene            13206..14156
                     /locus_tag="VFV59_01235"
     CDS             13206..14156
                     /locus_tag="VFV59_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324486.1"
                     /translation="MATTTFDQADARRAPTSLWRDTLGHVLRQRSAIIGLVLLAIILL
                     GALLADVLTPYGPTENLYSVPGAQRLAGPCIHLLGCPTDQPQFVMGLDQNARDVFARV
                     LHGARSSLQVGIVTVGIAIVLGTLIGSVAGYAGGWVDNLLMRIMDVLLAFPALVLAIF
                     LVTAFQTLDVPFFGDRLFNAMLAIGIVAIPVYARVMRASVLSVRERDFVTASRALGES
                     GRGILMRRILPNALTPLVVQGTLGIATAILEVAALSFLGIGAQEPTAEWGSMIGAVRN
                     QVFSAPHLILFPGGALALSVLAFNLLGDGLRDALDPRLNR"
     gene            14153..15256
                     /locus_tag="VFV59_01240"
     CDS             14153..15256
                     /locus_tag="VFV59_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324487.1"
                     /translation="MTISEMAPPRRRVGEVGERLLEVRGLQTSFHTSDGVVRAVTGVD
                     FHVNRGEVMGLVGESGCGKSVTSLSIMRLVSPPGRVEAGEVIFDGRDLLTLSAKEMRE
                     LRGDRISMIFQQPTSSLNPVYDVGMQLGEVLELHRNMKRRAARARAEELLAMVGIPDP
                     RRRIEAFPHELSGGMAQRVMIAMALACEPELLIADEPTTALDVTIQAQILDLMRVLQR
                     ETNTALILITHDLGVVAEMCDRVAVMYAGEIVEQTDVVELFDAPKHPYTQGLIGSVPV
                     LGQEQEQLATIPGSVPNLIDLPPFCRFAPRCRSRVEFEVELATERHPELLPVDENHSV
                     RCWMYHDADGSLRRPPLNARAPRQAAPMPEEDR"
     gene            15253..16242
                     /locus_tag="VFV59_01245"
                     /pseudo
     CDS             15253..16242
                     /locus_tag="VFV59_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
BASE COUNT         2296 a         6006 c         5743 g         2419 t
ORIGIN      
        1 cgggtgaccg acctcgtctc cgccgagcag gttcgcgcgc gccacgatcg cctgctgcga
       61 cggctccgcg aggtggccga tgcccacggc catgatcccg ggcgccttcg gatcgtcggc
      121 gtcaccaagg agcatccgct cgaggtcgtc gcggccgccg tggacgcggg gctcacccgg
      181 ctgggcgaga gccgggtcca ggaggctgag ccgaagagcg ccgccttccc ggcgatcgaa
      241 tggcacttca tcgggcggct ccagtcgaac aaggcgcgcc gcgccgtgcg ctccttcccg
      301 gtcatccact ccatcgactc gccggagctg ctccagcggg tggaccggat cgcggacgag
      361 gaggggcggc ggccgcaggt gctgctggag gtgaacgtct ccggcgaggc aaccaaagcc
      421 gggctccgac ccgaggatct cgacgagatc tcggcgccgg ccgtggcgga gctggtgggc
      481 ctgatgacca tcgcgcgctt cggcgcgtcg gaggaggagg cgcgtgccgt cttcgcgcgg
      541 ctccgcgcgc tgcgtgaccg gctccagcag cgtctcggcc gtgagctccc cgagctgtcg
      601 atgggcatga gcgccgatgc cgaggccgcc gccgccgagg gggcgacgta cgtccgcatc
      661 ggcaccgccc tgttcgggcc gcggcactga cggctgctat cctccgcgcg tgaccgccgt
      721 cctcatccgc ttcatcgagc tgctcgcgtt cgtcgtctgg ttcctgctga tcgcacgggt
      781 ggtgatgagc tggaccaacc cccggggcgg cggcgggctg accgccttcg tctaccaggc
      841 gaccgagccg atcctggcgc ccatccgccg ggtcctgccg cccagtggcg gcgtcgactg
      901 gtcgccgctg atcgcgatgc tgatcctcgg ggtcgtcgta cggctcgtcg cccagctctg
      961 acggagcgct gcgtggcgga gctgcaggtc atcgtcacgc cccgggcggc acgcgacgcg
     1021 atcgaggcgg tgcacgacgg cgccctccgt gtccgggtga ccaggccgcc ggccggcggg
     1081 gaggcgaatc gatcggtcat ccgcctggtg gccacggcgc tcgatgtccc gccctcctcc
     1141 atctccatcg cgtccggcca gcgcgcgcgc cacaagcggc tcgtcctggc gtcgctcgac
     1201 gaggcggagc tgcggcggcg gctggccgcc atcggccacg attgatggcc ccgcggcccg
     1261 acggctatac tcggtgcccg cggttccgcg ccatgggcga ttggctcagc tggttagagc
     1321 gcacggttca catccgtgag gtcactggtt cgaatccagt atcgcccacc acctcctccc
     1381 cctggacttc gtccggtggc tgaccgcgct cccgatgcgg cgccccgtgg caaccgcgtg
     1441 ccggcgtccg aggtccgcag catgttcgac cgcatcgcgc cgatctacga cgcgatgaac
     1501 acggtcatga cggccggcct ggatgcgcgc tggcgacgag cggcggccgc cgccgcaggg
     1561 ctcggccccg gcatgcgcgt cctcgatgtc gcctgtggca gcggcgccct ggctcgcgag
     1621 gtcgcgcggg cggtgggacc gaccggcgag gtcgtcggga tcgacctgtc cgagcggatg
     1681 ctcgagcgcg cgcggcatcg gccgccggat gcggccgcgg caccgatccg ctacgaggtc
     1741 ggcgacgcgc tggcgctccc cgaaccggac gccacgtacg acgccaccac gatcgccttc
     1801 ggcctgcgca acgtcgccga ctacgcgggg tgtgtccggg agatgcgccg cgtggtgcgt
     1861 cccgggggcc gggtggtggt gctggagctc gcgacgccgc gcgggggcat cggccgcctc
     1921 atcacccgca cctggttcga gcgggtcgtc ccgatcgtcg gccgactggc gggcggaggt
     1981 tccgcctacg gctacctgcc gtccagcgtg cgcggctatc cgccgccgga ggaggtcgcc
     2041 gcgctcctgc gcgatgccgg cctcgtggac gtgacctggc ggcggctgcg ccccgggctg
     2101 gtgacgctcc acgtcggccg ccggccatga cgggattgcg cgtcctgctg acgaccgagt
     2161 cctacctgcc gcaccagtcc ggcgtgacgg tcgccgtcga cgcgctcgcc cgcggcctca
     2221 aagcgcgcgg gcatgacgtg gtggtgctgg cgccgcggcc ggcgcgcggc gccaccctgc
     2281 ccgcggtcgg ctcacccggc ccggcgccac ggtacgcgtg gctggagtcc taccagctgc
     2341 cggcggtggc gccgcttggc taccgcatgc cgtggccgct gccgtggagc gccgggctgg
     2401 aggtggcgcg ggcgcatcgt ccgcaggtcg tccacgccca ctcgccgttc gtcaccggcc
     2461 tgatggcgcg acgactggcg cgcgccttcg aggcgccgct cgtgttcacg caccacacca
     2521 ggtacggcga ctacgcgcac tacgccgggc cggtggcccg gctggccggc tggggcgtcg
     2581 acgagtacct cgagcgcttc tggcgggcct gcacggcggt catcgccccg tcgacggacc
     2641 tggcggccga gatccgctcc agcgccccca gcctgcggat cgaggtcatc ccgaccggcg
     2701 tggacgtcgc cggactgggc gccgtcgacg cggtcgaccc gcggccgctg gccggatggc
     2761 cggacgacgc cgtggtggcg gcctcgctgg gtcgcctggc gccggagaag agcccgcata
     2821 ccctgctcga ggcgctcgcc atcgccgtcc cggacgtccc gcgcctgcgc ctgctgctca
     2881 tcggcggggg tccgtcggag gcggacctgc gacggcgggc ggcggcgccc gacctcgccg
     2941 ggaagatcgc cttcacgggg acgttgcccc gacccgaggc gctcgcccgg ctggcgggcg
     3001 cggacctgtt cgtgttcgca tcgcagaccg agacgcaggg gctcgtgctg gccgaggcgc
     3061 tcagcgtggg actgcccgcc gtggcgctgg agggtcccgg tgtgcgggac tcggtgcgcg
     3121 acgggatcga cggccgcgtc gtccgcgccg atgacccgca ccaacgagcc gccgagctcg
     3181 cgcaggcgat ggcgtcgctc gccgccgacg acacggcacg ggcggcatct gccgagcggg
     3241 cgcgtgcagg agcggaccgc ttctcgatcg agcggcgcat cgaggagacc gaggcgctgt
     3301 atcggtcagt gctccgctga gcgtggtcgg cgctcggtcg gatcggcctt gcaccgccta
     3361 cgacccggcg tacaatgccc ggcatgcgag tgagtctggt ggcccgcttc gtctcccacc
     3421 catcgatcga cttcggttcc ggccgtcgca gcgccctccg gccgcgcctc ggcctgagcc
     3481 gcgagcgcac ggtctaccac cgcgcgcgac gccgggagtc gaccgccacc atcggtgcgc
     3541 tccgcaccca cctgagcggc ttccgcttcc cggcggtcgg acgcgcctcg caccgtccgg
     3601 cccgtcgtcc agctcacccc ttcgggctgc cgcgcgctcg ccgcgcgtag gcgcccgggt
     3661 gcccatcctg cgatccgccc gagccacctc ggccggcggc gtcgtctacc gccggacgcc
     3721 ggaggcgggc ctcgaggtcg tgctggttca ccgtcgctcg ccgccgctct gggcgctgcc
     3781 caagggcacg ccggactccg gggagacgct cgaggagacg gcactgcgcg agactcgcga
     3841 ggagagcggg ctcgaggtcc agctggagca gccgatacgg gccattcggt acttcttcgt
     3901 ccgcgggacc acccggttcc ataagacggt ccacttctac ctgatgcgcg tgaccggcgg
     3961 cagccctgac gatcacgacg ccgagttcga cgaggtgcgc tggatgcccg tgaccgaggc
     4021 gctcgccctg ctgacccacg ccaccgagcg ctcggtggtc gagcgcgccg tcgagatgct
     4081 cgaggcaggc accggcggcc aagaggcgac ggcatgagcg tgggggagct ccgccgtacg
     4141 ccgcttgacg gcgtccatcg cgagctcggc gctcgcatgg tgcccttcgc gggctacgag
     4201 atgccggtgc agtacacctc catcctcgag gagcaccgca ccgtccggag cgccgtgggg
     4261 gtgttcgacc tctcgcacat gggggagctg gaggtgcgcg gagaggaggc gatggccttc
     4321 ctgcgctacg cgctggtgag cgacccgggc accctggaca tcggccaggc gcagtactcg
     4381 atgctgtgcg acgccgacgg cgggatcatc gacgacctca tctgctaccg gctgggcgag
     4441 gaccgatacc tcgtggtctg caatgcctcg aaccgtttcg ccgtggtcga gcagctcggc
     4501 tccctgctgg aacgtggcga cttcggcgcc gagctcgagg accgcagcga tcgcaccggc
     4561 ctggtggccc cccagggtcc caacgcggcc gcgctgctgg ccgagctgac ggacctcgac
     4621 ctgacggcga tcgccaacta ccacgccgcg gagggaacgg tggcagggat ccgctgcctg
     4681 gttgcccgca ccggctacac gggggaggac gggttcgagc tgttctgccc cgcgagcgac
     4741 acggtcgctc tctggcgcgc cgtcctccag gctggggagc gccacggggc gaagacgtgc
     4801 gggctcggcg cccgcgacac tctgcggctc gaggccggca tgccgctgta cggcaacgag
     4861 ctgggccgcg acgtgaaccc gtacgaggtc aacctcggcc gggtcgtcaa gctggagaag
     4921 ggcgagttcg tcggccgcgc ggcgctccag gccatccagc aggcgggtcc gtcgcgcaag
     4981 ctcatcggcc tgcagatggt ggacaacgcc atcccgcgcc acggctacga ggtccgcgcc
     5041 ggcggtcgcg ccgtcggctc cgtgaccagc ggcacgctgt cgccgacgct cggcacgaag
     5101 atcgccatgg ccctcgtcga ggccgatacg gccggcctcg gcaacgcgtt cgaggtggtc
     5161 gtccgcgacc gttcgtacgc ggcggagcag gtaaagttgc cgttctaccg gcgcccgcgc
     5221 gagtagcgcg cgccgaccgc ccaggaggga tcgaatctcc gatgtcctac cccgacgacc
     5281 tgcgctactc gaaggagcac gagtgggtcc gcgtcgaggg cacgcgcgcc accatcggca
     5341 tcaccagctt cgccgccgat gagctcggcg acatcgtctt cgtcgagctg ccggaggtcg
     5401 gcgccaccct gagccagttc gcgaccttcg gcgtcgtcga gagcgtgaag gccgtcagcg
     5461 acctcttcgc ccccgtctcg ggcgaggtgc tcgaggtcaa cgaggcgctg cgcgagagcc
     5521 ccgagctgct gaactccgac gccttcggcg acggctggat cgcgacgctc gagctctccg
     5581 acccgagcga ggtcgacacc ctgctcgacg cccaggccta cgcggcggag accgcctgac
     5641 ggcggcggac gaatgaccta ctcgccgcac acgccggccg atcgcgagcg gatgctcgcc
     5701 gccatcggcg tgggatcggt cgacgagctg ttcgacgaca tcccggcggc ggtgcgggcg
     5761 gacccctggt cgctgccgga tccgctgtcc gagcaggagg tgcgccacga gctcgcccgc
     5821 ctggcgaacc gcaaccgcat ccccgacgtg agcttcctgg gggcgggcgt gtaccgtcac
     5881 ctggtgccgg ccgtcgtcgg cgaggtcatc ggccgatccg agttcgcgac cgcgtacacg
     5941 ccgtaccagc ccgaggtcag ccagggcacc ctccagagca tctacgagtt ccagtcgctc
     6001 atctgtgagc tcaccggcat ggaggtcgcc acggcgtcgc actacgacgg cgccacggcg
     6061 accgccgagg cagccctcat ggcctgccgc ctcacgcgcc gggaccgcat cgcggtgtcc
     6121 gccggcctca acccggacta ccgccgcgtc ctcgagacgt attgctccgg cccggggatc
     6181 gaggtcgtca ccgtgccggt cgacctcgcc gagggtggca cgggcacgac cgatgcgagc
     6241 gccgccgaag cggcgctcgg cgatgacgtc gcctgcctgg tcgcggccca gcccaacttc
     6301 tacgggcagc tggagccgat ggccgagctc ggtgaggcgg cccacgccgc cggtgcgctg
     6361 ttcgtggccg tcgtcgagcc gacgtcgctg gccctgctgc aggcgcccgg cgactacggt
     6421 gccgacatcg tggcggccga gggccagccc ctcggcatcc ccgtgtcgtt cggcggcccg
     6481 tacgtcgggc tgatggccgc gcgcatgtcc tcggtgcggc agatgcccgg acgcctggtc
     6541 ggcgcgacgc gcgacgtcga cggccggcgc ggatacgtcc tgacgttaca ggcgcgcgag
     6601 cagcacatcc gccgcgaaaa ggcggcgagc aacatctgca ccaaccaggc gctcatggcc
     6661 ctggccgcca cgacgtacct caccgccatc ggtccgtccg ggctgcgcga ggttgccgag
     6721 ctgtcgacga cccaggcgcg ccacctggcc gcggcgctcg aggccgccgg cgtcggccgg
     6781 cgacgcttcg acgggccgta cttcgcagag gtcacgatgc aggtgccgga tgcctcgcgc
     6841 cggcacgccg cgctggtgga gcgggggatc gtcgccggct acctgccaga gcgcgacgag
     6901 ccggccctgc gtgacacgct gctgctcgcc gccaccgagc tgacgaccga cgagcacatc
     6961 gagcggctgg tgagcgcgct ggtgatcacc cgatgagcgt cgccgagccg ccggtcgacg
     7021 cctccggccg cgggtccgcc ttcagcggcg caccgaccga tgcgcaggag ctgaccgtgg
     7081 tcgagccgct catcttcgag tcctcgacgc cgggacgtcg ggccgtccgc ttcccatccg
     7141 ccagcgagcg cgcgcgcgag ctggccggcg ggcagccgga gctgcccgca tcggtccgca
     7201 gggctgcgcc gccgcggctg ccggaggtca gcgagctcga cctgctccgc cacttcaacc
     7261 ggctgtcgca cctcaaccac gcgatcgacc tggggttcta cccgctcggg tcgtgcacga
     7321 tgaagtacaa ccccaaggtc aacgagtggg ctgcccggct cccgggcctg gcggagtcgc
     7381 acccgctcga tccggaggag ctggcgcagg gcagcctgga gctcatgtgg ctgctgtccg
     7441 agctgctcaa ggagatcagc ggcctggacg cagtgagtct ccagccggcc gcgggggccc
     7501 acggcgagct gaccggcatg ctcatgaccc gcgccttcca ccgcgccaac ggcgagggcg
     7561 agcagcgcac caaggtgctg attcccgaca gcgcccacgg caccaacccg gcgaccgcca
     7621 cgatggtcgg ctaccaggcg gtccagatcc gctccaacga gcgcggcggc atcgacctcg
     7681 atgcgctgcg ggccgcgctc gggtccgacg tcgcggcgct gatgatcacg aacccctcga
     7741 cgctgggcat cttcgaggac cagatcgacc aggtcgtggc cgccgtcaag gaggcggggg
     7801 cgatcgccta catggatggc gcgaacctga acgccatcat ggggcgcttc cgagccggcg
     7861 acgccggctt cgacgtcatg cacttcaacc tgcacaagac gttcagcacg ccgcatggcg
     7921 gcggtggccc cggagccgga cccgtcggcg tcagcgagcg gctcgcgccg ttcctgcccg
     7981 cgccgctccc gctgctggtc gacggagacc cggcggaggt catcgccaac gcctgggccg
     8041 gccgctcgac gccgggcgcg cggttcgccc tcgacaacga ccgcccgcag agcatcggca
     8101 aggtgcgcac ggcctatggc aacttcggca tgttcgtgcg ggcctacacc tacatccgca
     8161 gcaacggcgg cagcgggctg gcgaccatct cggaggacgc cgtgatcgcg gcgaactacc
     8221 tccgcgtgcg cctggccgat gcgtacgacc tgccatacga ccggatctgc aagcacgagg
     8281 tcgtcttctc cggccgacgc cagaagcggg accacggcgt ctcgaccctc gacatcgcca
     8341 aggccatcct ggaccacggc atccacccgc cgaccatcta cttcccgctg atcgtcgagg
     8401 aggcgctcat gatcgagccg accgagaccg agagcgtgga gacactcgac cggttcgtgg
     8461 acgtgatgca cgagatcgcc gagcgcgcgg ccagcgatcc ggcatcgctg aaggccgcgc
     8521 cgacgacgac gccggtgcgt cgcctggacg aggcgacggc cgcgcgccac ccggacctgc
     8581 gctacatctt caaggacacg gcggagtcgc cgaggacggt cgcctcggag cagacagcct
     8641 gatcatttgg cgggcgcgag ccccacgcgt cccaccgacc gatccatcgt ccagaccaca
     8701 cggaaggcag gcctcggatg cgagttggcc gatccatcgg tcacggcggc cggctgatgc
     8761 tttccatgca tcacactccg acagcgtgag gatgcccgct ccgaattgaa cccggctgga
     8821 ccgccgggcg tactatggga aaccccacgg ccatcgaggc ccaccaggtg ccagtggaag
     8881 cgagacagcc cgacgacctc gtcgagcgca tgcaggccga cgacctggac gcgttcgagg
     8941 agttcttcaa cacgtacaag cggcccgtgt acgccacggc catggcgatc acacgcgatc
     9001 cgttccttgc cgaagaggtc ctgcaggact gcttcgtgaa agcgtaccgc gttcgcgaac
     9061 gcctcgatcc ggctcggtct ccgctgccct ggctccagcg cgtggcgatc aacctctgct
     9121 acagccgcgt cagccgtcgg cgggccatcg ccgagccgat caccagcctg atcaccaacc
     9181 tggtcgtcga ccttgccgcg cggccggacc aggtcgtcga gtcccgcgag gtgctggacg
     9241 tgttgcagcg gggcatcgac gcgctgccgc caaaacagca gaccgcaatc atcctctact
     9301 acctgcacgg ctactccctg gccgaggccg cggagattgc cgactgcaac gtcggcacca
     9361 tgaagtcgcg gctccactac gcgctgaagg cgctgcgggc acggctgccg gtggagcgcc
     9421 ggctgcccgt cgggacgccc gcggcggtgc gggtgaccga atgagctgcc gtcgggtctg
     9481 ccgcgagctg ctctggctgg cgcgcttcgg cgagttcggt cccagctcgc agccgcacct
     9541 ggaccacctg gcggggtgcc agcactgccg ggacgaggtc ggcttcgatc gggccctggt
     9601 ccagcagctg cgggtcgcgc tcgcggagcg cgtccaggac gcggcaccgt cgccggacgc
     9661 cttccaggcc atcctggccc ggacccggca gccggaacgc cgccgctggt tcgtctcgtg
     9721 gacgacggcc ctcggccgcc tgcgcttcgc gaccgccatg gcgggcaccg ggctcgcggt
     9781 cgtgctggcg ctcaacatgg aggtcgtgcg tgtcgacgtg ccggacacgg gcccggcgcg
     9841 tgggggcgag gcggtgctcc agcaggttcc gcgcatgccg cccggccaca cgaccctcgt
     9901 ggcgctcggc cagcgtggag acggggggcc ggcggcctcg cgccccgatc cggaggctgc
     9961 actgatgtgg gcggccacga tcccgctggc accggcgccg gccggtggcc aggaggagcc
    10021 ggccgaggag ccggccgagg cgccgagcgt cgagctgcgg gtcgccttcc gcccgttcca
    10081 gatccatgac ccgggcgcgg gcgcatcggc tccggacgat ccggacgacg ccggggtcgt
    10141 atgggcgccc gtggagctgt cgcagggaga gccttcctga accaaggggg agtgtgcgag
    10201 actacggcac cgtttcgcga ggacgcgccg gaccacccgg gacggcccga gcgagcgagc
    10261 ggtggtggtc cgcgtcagca tcaatttcgt ggaggagact ggatgcgaac ttggagactg
    10321 ttctccgtct tcgccgtcct ggtactcgtg ctggctgcct gcgggcagca aggtggcgcg
    10381 agcgcttcgg ccggtgagga gtcggaggcg ccgccgtcgt cctcggaggg cgcagagcca
    10441 tccgagggag cggcgttcga gccctcggcc taccccgagg acggaccggc cgagtgcggg
    10501 gccgataaca acccgtcgaa catcagcgag atccgcgccg aggacgagcg aaccgtcgtc
    10561 ttcaccctgt gcagccctga cgtcgccttc ctgtcgaaga tcgcgttcag ctcgttcgcc
    10621 atcgacgact cggcgtacct cgaggcgcac ggcgccgacg gcagcatcgt cgaccagccg
    10681 aacggcaccg gcccgtatcg gttcgacacc tggaaccgtg gttccgacat caccatgacc
    10741 gcgttcgagg actactgggg cgacgcgccc ctgagcccga ccgtcatctt ccgctggagc
    10801 gccgaggcgg cccagcgcct ggtcgagctg cagtccggca cggtcgatgg catcgacaac
    10861 cccgcacccg acgatttcgc ggccatcgag ggcgactcga acctcgagat cttcccgcgc
    10921 gagggcctga acgtcttcta catcggcttc aacaacacgt acgagccgtt caacgacgag
    10981 aaggtgcgcc aggccatcgc catgggcatc gatcgccagc ggatcgtcga caacttctac
    11041 ccgccggggt ccgaggtggc gacccacttc accccgtgcg cgatctcgtt cggttgcgag
    11101 ggtgaggact ggtacgagtt cgatccggaa ggcgccaagg cgctcctggc cgaggccggg
    11161 ttcccggatg gattcgatac ccagatccac ctgcgtgacg tcgttcgcgg ctacctgccg
    11221 ctgccgaccg ccgtggcggc cgacatccag gcgcagctcc aggagaacct gggcatcaac
    11281 gccgagatcg acgtccagga gtcgacgacc ttcatcgaca acgcggacgc cggcaacctg
    11341 cccggtatcc acctgctcgg ttggggcgcc gactatccgg atccgacgaa cttcctcgac
    11401 taccacttcg gtggcggtgc gtccgcccag ttcggcgaca agttcgacga catcaccgag
    11461 gccctggcca ccggtgcgtc gacggtcgac gagaccgagc gtgccgccgc gtacgagacg
    11521 gccaacaacg ccatcaagca gcacgtcccg atggtgccga tcgcccatgg cggctcggcg
    11581 acggtcttcc aggccggcct cgagggcgcg cactcgtcgc cgctgagcaa cgagcagatg
    11641 tccgtcatcg ccccgggcga cgacgatcag ctggtctgga tgcagaacgg cgagcctggt
    11701 ggcctctact gcgccgacga gtccgacggt gaggcactcc gcgtctgcga gcagatcaag
    11761 gagtcgctct acgcctacga gaccgccggc acggcggcca tcccggccct ggccgaggag
    11821 tgcacgccga acgacgacct gaccgtgtgg acctgcaccc tccgcgaggg cgtcacgttc
    11881 cacgacgggt cgacgctcga cgcgaacgac gtggtgctct cgtacgcggt ccagtgggat
    11941 gcggaccatc cgctccacat cggccgtgac ggcagcttca cctacttcgc ggccctcttc
    12001 ggtggcttcc tgaacccgcc cccggcggag tagggacgcc cgacgcgacc accggaaggg
    12061 ggcgccgcca cacacggcgg cgcccccttc gcccaccacc ccctcggcta ccatgagccg
    12121 ctagatgctc cagttcatcc tgcgccggct gctcctcagc atcccggtgc tgttcggcat
    12181 catcttcctg gtcttcgcgc tcgcccggct gctgccgggc gatccgtgtc gtgcggccct
    12241 gggcgagcgt gcgaccgatg cgatctgcaa tgccttcaac gcgcgcttcg gcctgaacga
    12301 gcccattccc gtccaattcg tgacctacct cggtcagctg gcacgcggcg accttggcga
    12361 ctcgatccgc cagggccgtc ccgtgtcgga gatcctgctg gagcgcctgc cggtcaccct
    12421 ggagctgacc ctgttcgcgc tgctgttcgc ggtgatcgtc ggcgtgaccc tgggccggct
    12481 ctcggcgcgc cgccgcaact cgccgctcga cgtcggcacc atgctggtcg ccaacctggg
    12541 cgtgtcgatt ccggtcttcg tgctcggcct gctgctggcc tacctcttcg ccgtggtcct
    12601 gcgcggtaca ccgatcgcgt tgccgccgtc cggtcagctg tcgcccggcg tcgcgcacca
    12661 gtcgctgcag caggcctggg ggctcgggcc ggtcgacggc ttcctgggcg cgctgctgac
    12721 gttcgtctcg aacatggtga tcttctcgtc actgatcacc ggccagtgga cgccgttcct
    12781 cgacggcctc aagcacatcg tcctgcccat ggtcgcgctg ggcacgatcc cgatggcgat
    12841 catcgcgcgc atgacccgct ccagcatgct cgacgtgctc ggcctcgact acatccgcac
    12901 cgcgcgcgcc aagggggcgc cggagcgcct ggtgatgggc cgccacgcgt tccgcaacgc
    12961 gctgctgccg gtcgtcaccg tcgtcggcct gtcgctgggg gccttgctgt ccggcgccgt
    13021 cctgaccgag tcgatcttcc tgctgcccgg cctcgggcgc acgctcttcg aggccatcac
    13081 cggccgtgac tacatcgtcg tgcaggcgct cacgctggtg accgccgtgt cgtacgtcct
    13141 gatcaacctc gtggtcgacg tctcgtacgc ctacctcgac ccacgcatcc ggctgcgctg
    13201 acccgatggc caccacgacc ttcgaccagg ccgatgcgcg acgcgcgccg accagcctgt
    13261 ggcgcgacac gctcggccac gtcctgcgtc agcgttcggc gatcatcggg ctggtgctgc
    13321 tcgccatcat cctgctgggt gcgctgctgg ccgacgtgct gacgccgtac ggcccgaccg
    13381 agaacctgta cagcgtcccg ggcgcacagc gcctggcggg accgtgtatc cacctcctgg
    13441 gttgcccgac cgatcagccg cagttcgtga tgggcctcga ccagaacgcg cgcgacgtct
    13501 tcgcgagggt gctgcacggg gcgaggtcgt cgctgcaggt gggcatcgtc acggtcggca
    13561 ttgcgatcgt gctcggcacc ctcatcggct ccgtggccgg ctacgccggc ggctgggtcg
    13621 acaacctgct gatgcgcatc atggatgtgc tgctggcctt cccggccctg gtgctggcca
    13681 tcttcctggt gaccgccttc cagacgctgg acgtgccgtt cttcggcgac cggctgttca
    13741 acgccatgct cgccatcggc atcgtggcga tcccggtcta cgcgcgcgtc atgcgcgcat
    13801 cggtcctgtc ggtgcgtgag cgcgacttcg tgaccgcctc gcgcgcgctg ggggagtccg
    13861 gacgcggcat cctcatgcgc cggatcctgc cgaacgcgct cacgccgctc gtggtgcagg
    13921 gcacgctcgg catcgcgacc gccatcctgg aggtggccgc gctctcgttc ctgggtatcg
    13981 gagcgcagga gccgaccgcc gagtggggct cgatgatcgg cgccgtgcgc aaccaggtct
    14041 tcagcgcacc gcacctgatc ctcttcccgg gcggtgccct ggcgctcagc gtcctggcgt
    14101 tcaacctgct cggtgacggc ctgcgcgacg cgctcgaccc gaggctgaac cgatgacgat
    14161 cagcgagatg gcgccaccgc gacgccgcgt cggcgaggtc ggggagcggc tgctcgaggt
    14221 ccgcgggctg cagaccagct tccacacctc cgacggcgtc gtgcgtgccg tcacgggcgt
    14281 ggacttccac gtcaaccgcg gcgaggtgat gggcctggtg ggcgagtccg gctgtggcaa
    14341 gagcgtcacc agcctctcca tcatgcgcct ggtgtcgccg cccggccgcg tcgaggcggg
    14401 ggaggtgatc ttcgacggcc gtgacctgct gacgctgtcc gcgaaggaga tgcgcgagct
    14461 gcgaggcgac cggatcagca tgatcttcca gcagccgacc agcagcctca acccggtcta
    14521 cgacgtcggc atgcagctgg gcgaggtgct cgagctgcac cgcaacatga agcgtcgcgc
    14581 cgcgcgggcc cgggccgagg agctgctggc gatggtcggc atccccgatc ccaggcgccg
    14641 gatcgaggcc ttcccgcacg agctgtcggg cggcatggcg cagcgcgtca tgatcgccat
    14701 ggcgctggcg tgcgagccgg agctgctcat cgccgacgag ccgaccaccg cgctggacgt
    14761 gaccatccag gcccagatcc tggacctgat gcgggtcctg cagcgcgaga ccaacaccgc
    14821 gctgatcctg atcacccacg acctgggcgt ggtggccgag atgtgcgacc gggtggccgt
    14881 catgtacgcc ggcgagatcg tggagcagac cgacgtggtc gagctgttcg acgcccccaa
    14941 gcacccgtac acgcagggac tcatcggctc ggtgccggtg ctggggcagg agcaggagca
    15001 gctggccacg atccccggca gcgtgccgaa cctcatcgac ctgccgccgt tctgtcgctt
    15061 cgcaccgcgc tgccgctcgc gcgtcgagtt cgaggtcgag ctcgcgaccg agcgccatcc
    15121 cgagctgctg ccggtggacg agaaccacag cgtccggtgc tggatgtacc acgacgccga
    15181 tggcagcctg cgccggccgc ccctgaacgc ccgagcgccc cgccaggcgg cgccgatgcc
    15241 cgaggaggac cgatgacgac ggacggttcc cgcccgctgg tcgaggtcga gaacctggcc
    15301 gagttctttc cgatccacgg tggcgtgctc catcgcaagg tcgccgacgt caaggcggtc
    15361 gacgacgtca gcttcaccat ccgacgcggc gagaccctgg gcctggtggg ggagtccgga
    15421 tcgggcaaga cgacggtcgg ccgggtcctg ctgcggctga tcgagccgac cgccgggcgc
    15481 atcgtgttcg acgggaccga catcaccaag ctgtccgggc cgaagctcaa gtccttccgc
    15541 cggcgcatgc agatcatctt ccaggacccc ttcgccagcc tggatccgcg gcagcagatc
    15601 ggcgagagca tctcggaggg cctccgcatc cacggcctgg gcaacgcctc cgagcgtcgg
    15661 gagcgcgtcc tgcggatcat ggacctggtc ggcctgcagc cgtaccacgc gcgccgctac
    15721 ccgcacgagt tcagtggggg gcagcggcag cgcatcggca tcgcgcgggc gctcatcctg
    15781 gagcctgacc tgatcgtgtg cgacgagccg gtcagcgccc tggacgtcag catccaggcg
    15841 caggtcctca acctcctgcg cggcctccag cgcgacctcg gcctgaccta cctgttcatc
    15901 gcgcacaaca tgggcgtggt cgagcacatc agcgaccgcg tggcggtgat gtacctgggc
    15961 gcgctgatgg agctgtcgga gtcgacagag atctaccgga caccgatgca cccgtacacg
    16021 atcgcgctgc tctcggccgt gccggctcca tcgccgcggg gtcgggagca ccggcggcgc
    16081 atcatcctgc tcggcgacgt cccatcgccc gtcaacccgc cgtccggctg ccggttccac
    16141 acccgctgct ggctgcggcg cgagctcggc aaccccgagc agtgcgtgac ggagcgaccg
    16201 cccctgcggt cgatcaacgg ggcggagcac caggtggcct gccacttcgc ggaggagctg
    16261 ctgccgggtg agcgccgcga cacgctgatc cgcgatgcgg cgcactcgag ctcgccgttc
    16321 gagacgagcg acgacgaggc ggtgacggcc ggtcccacgc ccggcgaccg cggcggtccc
    16381 ttcgacacgc cgaccgacga ccaccgcgac gacgccatga gccggctcta ggaccgcggc
    16441 tcgcgtggag atccggatcg cgct
//
LOCUS       DASTYP010000025         4921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_803043, whole genome shotgun sequence.
ACCESSION   DASTYP010000025 DASTYP010000000
VERSION     DASTYP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803043"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFV59_01250"
     CDS             <1..363
                     /locus_tag="VFV59_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324488.1"
                     /translation="LTLPIVARHADAWHAFFPDTVEEVVPAVEALLAHCAAIGRDPYE
                     IEWSVGVEPTDIGRFLEQDAERYVAMGFTQFTLGFNGPSWSVEGGAAFLAWRDRMNRE
                     RGVAGAAPGAVLVGREYR"
     gene            396..830
                     /locus_tag="VFV59_01255"
     CDS             396..830
                     /locus_tag="VFV59_01255"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5324489.1"
                     /translation="MRITVSTPIDRPVAGVWRWYAIDHVRNHPRWDPDMELEQISDGP
                     IGLGTRIRRRNRHFGEPIEGEMEIVEWEPERAMGVHIRDANADSRGRVTFEALGPNAT
                     RLGIEADFDTLDDSRADQLRPLMERTAANLRRLIESEVEPGA"
     gene            complement(1022..1903)
                     /locus_tag="VFV59_01260"
     CDS             complement(1022..1903)
                     /locus_tag="VFV59_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5324490.1"
                     /translation="MTADSLTDALPRTVARQARGAFHSVWDATMLFVDDQGLSWAGAI
                     GLYLFLSVPPFMVAVTWMAGAFVGNSGAESFVVEQVSKYLPAEQKLLQGVVGSAPGNG
                     AAALASVLLLLFSGSRAFAAMTSAVNVLWRRVDELTFVRRQALRIGMLMVTVVLLALA
                     AMGEALVRAIAGATTGGDGMWLLDWQILPSVLLATFLLIAYKLLPREPVSWWHAAIGA
                     IGATVGIRLAQAGLGVLAQAGTFRTPYGDLAGVALIATWALVVGVIVLWCAALVAALD
                     GKRPGDGERETRFSRAE"
     gene            2013..2630
                     /locus_tag="VFV59_01265"
     CDS             2013..2630
                     /locus_tag="VFV59_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5324491.1"
                     /translation="MADLEAEPLPMPDPELILADVEQLKAIADPLRLRLLELMTDDAQ
                     HPWSAKELAERLGTGQTKLYHHLALLEERGLIRVAGTRLVSGIQEKRYQVSALSFRVD
                     RLLLTGDDTEAALGTALDAIFEKARTEILSSARRGAIRLEEQDPHRRRMALWASHARL
                     SPEGLAQVMQKIEELASIDDHNEPDGTDYGLVLAFYPRPTEEHDR"
     gene            2627..3904
                     /locus_tag="VFV59_01270"
     CDS             2627..3904
                     /locus_tag="VFV59_01270"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324492.1"
                     /translation="MTERSMGVRDVLRIRDYRSIFGGQVVSDIGDGVTFLLLLLVIND
                     LTGSTTALALMAICEALPQFTVGLFAGVYVDRWNRRTVMLAADLLRAVVVIGFAVAAV
                     EGWVPLLYVLAFVQSSISTFFRPARGAMLPRIVPREGLPAANSLAQGSMVIGGVIGTG
                     IAGLLYGTFGVAYLGFAIDAATFLVSMVFVSRIPSIVGRIQREAHDAPQSNVLRSLLE
                     GLAIFRHSRVLSGVLLAAAVSMLGLGAVNVLFVPLIVDDLKVAATWMAGIELAQTSAM
                     ILAAGVVPMLLRRFAPTTIVTGALLGVGLCIGMMSGVSAVWQVIVVLFIVGWMVTPFQ
                     ATVQTIVQTTATDATRGRVVSLLQASMSTASVVSMAVGGVLADLIGIRAVYAGAAVVV
                     LGAAVVAMLMFRGVPRRPLVGATVPVEAEAAAP"
     gene            complement(4078..>4921)
                     /locus_tag="VFV59_01275"
     CDS             complement(4078..>4921)
                     /locus_tag="VFV59_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324493.1"
                     /translation="VLELEVLEHAGAARDEVDVATPADWERIEELARELGRWSQVASA
                     ARVRAVYLAAEDPAAALPGLQQAIEVASAHGLREQAGWADYSRAEALWLLDRCDEALG
                     AGFRALEVAERFAYERLAFRTFVILLAIAADRGDREIADRWDRWWAVAEPHFPATFSP
                     YAVVVHAGIAVWRRLARGEAPAEPDASVVDAIIPMINPHYLAAAESVIGVWADSGRAD
                     LVTAAAQRLAAIAADADATALIRTAAALARAHAGDAGAAREAAEMAASIPAPWWERRA
                     RSLA"
BASE COUNT          714 a         1769 c         1732 g          706 t
ORIGIN      
        1 ctgaccctgc cgatcgtcgc ccgccacgcc gatgcctggc acgcgttctt ccccgacacc
       61 gtcgaggagg tcgtgccggc ggtcgaggcg ctgctcgcgc actgcgccgc gatcggtcgc
      121 gacccgtacg agatcgagtg gtccgtgggc gtggagccca ccgacatcgg acggttcctt
      181 gagcaggatg ccgaacgcta cgtggcgatg ggcttcaccc agttcacgct cgggttcaac
      241 gggccgtcgt ggtccgtcga gggtggcgcg gcattcctgg cgtggcgcga ccggatgaac
      301 cgcgagcgag gggtcgccgg cgcggcgccg ggggcggttc tcgtggggcg ggagtatcgg
      361 tgacgccgct ggacggcgct gatagggttc ggcgcgtgag gatcaccgtc tcgacgccca
      421 tcgaccgccc ggtggccggc gtctggcgct ggtacgccat cgaccacgtc cgcaaccatc
      481 cacgctggga tccggacatg gagctcgagc agatctccga cggaccgatc gggttgggca
      541 cgcgcatccg caggcgcaac cgtcacttcg gcgagccgat cgagggcgag atggagatcg
      601 tggagtggga gcccgagcgg gccatgggcg tccacatccg cgacgcgaac gcggactcgc
      661 ggggccgggt cacgttcgag gcgctcgggc cgaacgctac gcggctcggt atcgaggcgg
      721 acttcgacac cctcgatgac tcgagggccg accagctccg gccactgatg gaacgcaccg
      781 cggcgaacct gcgccgactc atcgagtccg aggtcgagcc gggagcctga gctgccccgc
      841 gcctcctcgg ggcgcggcgc cggttgctcc ccgccggagc taacggcgaa gttgcgccaa
      901 tcttggtccg cttcgtggtc atccgttgcg ccggggcgca acaggcggcc acacagggcg
      961 cggtctgggt cgctgcgtgt ccgttagttc cgctcccggg caaccgcccc cagggctacc
     1021 gctactccgc gcgcgagaac cgcgtctccc gctccccgtc ccccggacgc ttcccgtcca
     1081 gtgccgcgac cagcgccgca caccacagca cgatcacgcc caccaccagc gcccaggtcg
     1141 cgatgagcgc gacgcccgcc aggtcgccgt acggcgtgcg gaaggtgccc gcctgggcca
     1201 ggacgccgag tcccgcctgg gccagccgga tcccgacggt cgcgccgatc gcgccgatgg
     1261 cggcatgcca ccacgacacc ggctcgcgcg gcagcagctt gtaggcgatg agcaggaagg
     1321 tcgcgagcag cacgctggga aggatctgcc agtcgagcag ccacatcccg tcgccgccgg
     1381 tggtcgcgcc ggcgatcgcg cggaccaacg cctcccccat cgcggccagt gccagcagca
     1441 cgaccgtcac catcagcatc ccgatgcgca gcgcctggcg tcgcacgaag gtgagctcgt
     1501 cgacgcgccg ccagagcacg ttgacggcac tggtcatggc ggcgaacgcg cggctgccgc
     1561 tgaagagcag caacaggacc gatgcgagcg cggccgctcc gttgcccgga gcggatccga
     1621 cgaccccctg cagcagcttc tgctcggccg gcaggtactt cgacacctgc tcgaccacga
     1681 acgactcggc gcccgagttg ccgacgaacg cgccggccat ccacgtcacg gcgaccatga
     1741 acggcgggac cgagaggaac aggtacagcc cgatggcgcc cgcccacgac aggccctggt
     1801 cgtcgacgaa gagcatcgtc gcgtcccaga ccgagtggaa ggcaccgcgt gcctggcgcg
     1861 cgacagtgcg cggcagggca tcggtcaggc tgtcggcggt cacgcgggca ggagggcacg
     1921 ccggatgcca gcagggtttc agggcttgac agccacgacc gttcacctgc actatcacgt
     1981 tcatcctttc tcgaacggta gaggtggagt gaatggccga tctcgaggcg gagccgctgc
     2041 cgatgccgga cccggagctg atcctggccg acgtcgagca gctgaaggcg atcgccgatc
     2101 cgctgcgcct gcggctgctg gagctcatga ccgatgacgc ccagcatccc tggtcggcca
     2161 aggagctggc cgagcggctg ggcaccgggc agacgaagct ctaccaccat ctcgccctgc
     2221 tggaggagcg cgggctgatc cgcgtcgcgg gcacgcgcct ggtcagcggc atccaggaga
     2281 agcgctacca ggtcagcgcc ctctccttcc gcgtggaccg gctgctgctg accggcgacg
     2341 acacggaggc ggcgctgggc accgcgctcg acgcgatctt cgagaaggca cgcacggaga
     2401 tcctctccag cgcccgccgg ggcgcgatca ggctcgagga gcaggacccg caccgccggc
     2461 gcatggccct gtgggcgtcg cacgcgcgcc tctcccctga aggcctcgcg caggtcatgc
     2521 agaagatcga ggagctggcc tccatcgacg accacaacga gcccgacggc accgactacg
     2581 gcctggtcct ggccttctat ccccgcccga ccgaggagca cgaccgatga ccgagcgatc
     2641 gatgggggtc cgcgacgtcc tgcgcatccg cgactaccgc tccatcttcg gcggccaggt
     2701 cgtctcggac atcggcgacg gcgtgacctt cctgctcctg ctgctggtga tcaacgacct
     2761 caccggctcg accacggcgc tggccctgat ggccatctgc gaggcgctcc cccagttcac
     2821 ggtcggcctc ttcgcgggcg tgtacgtgga ccgatggaac cgccgaacgg tgatgctggc
     2881 cgccgatctg ctgcgcgccg tggtcgtcat cgggttcgcg gtggcggcgg tcgagggctg
     2941 ggtgccactg ctgtacgtcc tcgccttcgt gcagtccagc atcagcacct tcttccggcc
     3001 cgcgcggggg gcgatgctcc cgcggatcgt gccgcgcgaa ggactgcccg ccgcgaactc
     3061 gctggcgcag ggttcgatgg tcatcggcgg tgtgatcggc accgggatcg ccgggctgct
     3121 gtacgggacg ttcggcgtcg cctatctcgg cttcgcaatc gacgcggcga cgttcctggt
     3181 ctcgatggtc ttcgtgtcgc gcatcccatc gatcgtcggg cgcatccagc gggaggccca
     3241 cgacgcgccg cagtccaacg tgctgcgctc cctgctcgag gggctcgcca tcttccgaca
     3301 ctcccgcgtg ctgagcggcg tgctgctggc ggcggccgtg tccatgctcg gactcggggc
     3361 ggtgaacgtg ctgttcgtgc cgctcatcgt cgacgacctg aaggtggcgg cgacctggat
     3421 ggccggcatc gagctggcgc agacctccgc gatgatcctg gctgccggcg tggtgccgat
     3481 gctcctgcgc cggttcgcgc cgacgaccat cgtgaccggc gcgctcctcg gggtcgggct
     3541 gtgcatcggc atgatgtccg gcgtaagcgc ggtgtggcag gtgatcgtcg tcctgttcat
     3601 cgtcggctgg atggtcaccc ccttccaggc aaccgtgcag accatcgtcc agaccaccgc
     3661 gaccgatgcg acgcgcgggc gcgtcgtctc gctcctgcaa gcctcgatgt cgacggcgag
     3721 cgtcgtctcc atggccgtcg gtggcgtgct cgccgatttg atcgggatcc gcgcggtcta
     3781 cgccggtgcg gcggtggtgg tgctgggcgc ggcggtcgtg gcaatgctca tgttccgcgg
     3841 cgtcccccgg cggccgctgg tcggggccac ggtcccggtg gaggccgagg cggcggcacc
     3901 gtagcggtcg tccggctgtc ggttgcctcc cagcgaagct aacggccaag aagagccgct
     3961 tcccggcaca tccgtgaccg cccgttgcgg cggggcgcaa ccggaggcag cggagacgcc
     4021 gtccaggcag gctgcgcctc cgttagttcc ggccgggagc aactcccagc acggggctca
     4081 ggcgagcgag cgagcgcggc gctcccacca cggcgccgga atggacgccg ccatctcggc
     4141 cgcttcgcgt gccgcaccgg cgtcccccgc atgcgcccga gcgagcgcgg cggccgttcg
     4201 gatgagcgcc gtggcatcgg cgtccgcggc gatggcggca agccgctgag cggccgccgt
     4261 cacgaggtcg gcccgacccg aatcggccca gacgccgatc accgactcgg ccgcggccag
     4321 gtagtgcgga ttgatcatgg ggatgatggc gtcaacgacg gacgcatcgg gctcggcggg
     4381 agcttcgccg cgtgccagcc gacgccagac ggcgatcccg gcgtgcacca cgacggcgta
     4441 tggcgagaag gtcgcgggga agtgcggctc ggcgacggcc caccagcgat cccaacggtc
     4501 ggcgatctcc cggtcacccc gatcggccgc aatggccagc aggatgacga acgttcggaa
     4561 ggcgagccgc tcgtatgcga accgctcggc aacctccagc gcacggaagc cggctccgag
     4621 cgcctcgtcg catcgatcca gcagccacag cgcctccgcc cgcgagtagt ccgcccaacc
     4681 cgcctgctcg cgcaggccat gcgccgatgc gacctcgatc gcctgctgga gtccgggcag
     4741 cgccgccgcc gggtcctctg cggcaaggta gacggcgcgc acgcgagccg ccgatgcgac
     4801 ctgcgaccag cggcccagct cgcgcgccag ctcctcgatg cgctcccagt cggccggggt
     4861 cgcgacgtcg acctcgtccc gggccgcgcc cgcgtgctcg agcacctcga gctccaggac
     4921 c
//
LOCUS       DASTYP010000026         7973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_948778, whole genome shotgun sequence.
ACCESSION   DASTYP010000026 DASTYP010000000
VERSION     DASTYP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948778"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..223
                     /gene="tatA"
                     /locus_tag="VFV59_01280"
     CDS             8..223
                     /gene="tatA"
                     /locus_tag="VFV59_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5324494.1"
                     /translation="MPVPGPWELILLLVIVLIIFGPGKLPDIGNAVGKGIREFRKAST
                     ELEESVRGEKKSTTGDRPASDSADRTS"
     gene            279..986
                     /gene="tatC"
                     /locus_tag="VFV59_01285"
     CDS             279..986
                     /gene="tatC"
                     /locus_tag="VFV59_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5324495.1"
                     /translation="MTDEATMTLLEHLEELRRRIIVIAVAILLASIAGFFLADALIAL
                     IRAPLPDDGASLIQTTIGEAFGVRMQMALMAGVAIAMPVILYEVWAFVTPGLTKHERR
                     LVWPLLGAAIILFAAGLALGYLLIPVAVNFLLDFSLEGVEPLLGLGDYVGFMTTFLLA
                     FGLALEFPVVLYLLARLGILSYAFLAKRRRYAIILIVLFAIVITPGDIVIGSATLAIV
                     MYGLFEITLQLIRMLGR"
     gene            991..1956
                     /locus_tag="VFV59_01290"
     CDS             991..1956
                     /locus_tag="VFV59_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEU5324496.1"
                     /translation="MDAPVWLLTDPAMDRHATHGHPERPERRPAAAAGVRDAAGGDLA
                     EPAVIPAADDAIERIHGAAYVERVREADAAGGSWLDPDTYLVSGSLEAARLAVGATLQ
                     AARAAATGEAEIAFAAVRPPGHHAAARRASGFCLFNNVAVAAAELRAAGLAQRIAIVD
                     WDVHHGDGTQAIFDDDPDLCYASTHQWLLYPGTGDRHDRGVGPAEGTKHNVPLLPGKG
                     DAAFVTAWRDELLPAVEAFGPEAILVSAGYDGHHDDPLAHLEITEDGYEQVARLLGAT
                     AARLGLPGVTLALEGGYDLDALRRSVAATVRGLRHGRRSAHEVAR"
     gene            1998..2936
                     /gene="rapZ"
                     /locus_tag="VFV59_01295"
     CDS             1998..2936
                     /gene="rapZ"
                     /locus_tag="VFV59_01295"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU5324497.1"
                     /translation="MARSASIEGKAPEQAGTPAEAGETAVDHVLVISGLSGAGKSQAA
                     KLFEDLGYFVVDNLPPALLDDFLALRRADPERYRRSALVLDVRSGDPAPAIERAASAL
                     RRDQGRLEVIYLEASDATLISRFSETRHRHPLEGTRSGVQASLAEERELLTPTREMAN
                     HVIDTSGLSIGQLKDRLFALVPRTDQDDALQIDIVTFGFKYGIPLEADLVFDVRFLTN
                     PYWEPHLKPLSGLEAPVRDFVLGQPKAMRFLELVHELLELTVPAYRAEGKGRLRIALG
                     CTGGYHRSIALAEELATSLRDLPGSSVSVFHRELER"
     gene            2933..4252
                     /locus_tag="VFV59_01300"
     CDS             2933..4252
                     /locus_tag="VFV59_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEU5324498.1"
                     /translation="MRLGRLPRWMYPGMHLKRWLLLLFVGIAILGLGAAIFIRDLYRS
                     TDADEIAIVYWLTGAWLEPQIRAALVAALGVVLTVIGMWGLMRSVVSPFVARGDSVLE
                     VLYTKRYLARGPRIVAIGGGTGLSTLLRGLKGYSANITAVVAVADDGGSSGQLRQQLG
                     IIPPGDIRNCIAALADAEPLMTQLMQYRFPAGSGLDNHAFGNLFIAAMTAVTGDFEEA
                     VRESNRVLAVRGQVLPATTVPLNLSARLQSGRILVGQSGIAHAEEPIEQVFIEPNDVR
                     ANGEALERILEADMVVIGPGSLFSSILPNLLIPDVRDALAAAPGLRIYVCNVATQPGE
                     TPDLTASQHLTTLFEHVGDDLIDCVIVNRGSARRPEGWRAQPVEVDVRRLEELPVVIV
                     EEDVIDPANAHRHDPAKLAATLMRLQQEDRVERPRQRRVRRPTATAS"
     gene            4258..5109
                     /gene="whiA"
                     /locus_tag="VFV59_01305"
     CDS             4258..5109
                     /gene="whiA"
                     /locus_tag="VFV59_01305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU5324499.1"
                     /translation="MLVAGEVRGELARIQPARACCRRAELHGLLAGSRDGVLRTLDVA
                     TARTAMQLASAVGVALPAPPRGAASTSRRAGGRHHLAVPLDLDALGAWSWTAAPACDR
                     RSYLRGALLGAGSISFGRSGTHVEFVLADDEAGEALQAALASVDVRALRSTRRGRSVV
                     YLKGQEEVAALLRLVGANRGLLELETHRVGADVRARLNRLLNAEEANLGRTVRAADRQ
                     MAAIARLEADGTLARLGHALRDTAATRRRMPDADLDTLAAALGVSRSAVNHRLRRLVE
                     LATGEED"
     gene            5109..5876
                     /gene="tpiA"
                     /locus_tag="VFV59_01310"
     CDS             5109..5876
                     /gene="tpiA"
                     /locus_tag="VFV59_01310"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5324500.1"
                     /translation="MRTPVIAGNWKMNPPTLEEATALALAIRQLADELPDVTTVVCPP
                     TIWLPDVARAARGGRLHTGAQTMHFEEKGAFTGETSPLMLRAYADCVILGHSERRQFD
                     CETDERVGRKVASAVAHGLRPIAAVGETAEQRRAGETAEVVDRQVRAALGNLERLAGT
                     GLVVAYEPVWAIGTGDAASPDDAQEVAAQIRAILRELDSAAADDIPIQYGGSCTADNA
                     PDFFAMPDIDGALVGGASLKADNFGGILRAAADAVAA"
     gene            5873..7393
                     /gene="gpmI"
                     /locus_tag="VFV59_01315"
     CDS             5873..7393
                     /gene="gpmI"
                     /locus_tag="VFV59_01315"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU5324501.1"
                     /translation="MTVVLVVLDGFGMGDDPKRSAILAARMPNWRRLLAEFPNARLAA
                     SGEAVGLPPGQMGNSEVGHLNLGAGFRVLQDLPRINAAIADGSFFANPVLVSTCRGVL
                     ERGGRLHLMGLVGPGGIHAVDEHLVAMVDLAARVGLPEDRVLFHAFTDGRDTPPRSAD
                     RFLPELEARVAGRARVATVTGRYWAMDRDRRWDRTKRAYDAIVHGQGLRAVDATAAIA
                     VAYARDEGDEFIQPTVLDAAVPMTDGDAAIHLNFRADRARQLTAALTLDEFDGFDRGG
                     RRPDVEVATLTEYQAPDELPVRVAFPPLQIDSLAAHLDRLGLTQLHVAETEKYAHVTY
                     FFNGGVEDPFAGEDRILIPSNRAVATYDQAPEMSAAPITDAIVEGIEGGVYTFLVANY
                     ANPDMVGHTGDWDATVSALEYLDGCLGRVADAALRTSSPLMITADHGNAEEMRDPSGA
                     PQTKHTTSPVPVVLVSAAHGGRTLHDGELRDVAPTLCELLGIPPGPEMTGHSLLDR"
     gene            7464..7685
                     /gene="secG"
                     /locus_tag="VFV59_01320"
     CDS             7464..7685
                     /gene="secG"
                     /locus_tag="VFV59_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU5324502.1"
                     /translation="MQTPLLIAQMILAVALMAAILLQQRGTGLGGAFGGEVTAYRSRR
                     GIERTLFRLTIVLAVLFVAFSLLNLFQQG"
     gene            7755..7841
                     /locus_tag="VFV59_01325"
     tRNA            7755..7841
                     /locus_tag="VFV59_01325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7789..7791,aa:Leu,seq:cag)
     gene            7850..7934
                     /locus_tag="VFV59_01330"
     tRNA            7850..7934
                     /locus_tag="VFV59_01330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7884..7886,aa:Leu,seq:gag)
BASE COUNT         1108 a         2940 c         2757 g         1168 t
ORIGIN      
        1 tgagttcatg ccggttcccg gaccatggga gctcatcctg ctgctggtga tcgtcctgat
       61 catcttcggc cccggcaagc tgccggacat cggcaacgcc gtcgggaagg ggattcgcga
      121 gttccgcaag gcctccaccg agctcgaaga gtcggtgcgc ggcgagaaga agtcgacgac
      181 cggcgaccgg ccggccagcg actccgccga ccgcaccagc tgaccgggcc ccgccccggt
      241 caccgctcca gggcgcccac tgagccgccg cctgcccgat gaccgacgag gccacgatga
      301 ccctcctcga gcacctcgag gagctgcgtc gccgcatcat cgtcatcgcc gtcgcgatcc
      361 tcctcgcctc catcgccggc ttcttccttg ccgatgcgct catcgccctg atccgcgcgc
      421 cgctgcccga tgacggcgcg tcgctgatcc agaccaccat cggtgaggcg ttcggcgttc
      481 ggatgcagat ggcgctcatg gcgggcgtcg ccatcgcgat gccggtgatc ctgtacgagg
      541 tctgggcgtt cgtgacgcca ggcctcacga agcacgagcg ccgcctggtc tggcccctgc
      601 tcggcgccgc gatcatcctg ttcgccgcgg gcctggcgct ggggtacctc ctgatcccgg
      661 ttgcggtgaa cttcctgctc gacttctcgc tggagggcgt cgagccgctg ctcggcctgg
      721 gcgactacgt cggcttcatg accacgttcc tgctggcgtt cggcctggcg ctcgagttcc
      781 cggtcgtcct ctacctgctg gcgcgcctcg ggatcctgtc gtacgcgttc ctggcgaagc
      841 gccgtcgcta cgccatcatc ctgatcgtcc tctttgccat cgtcatcacg cccggggaca
      901 tcgtcatcgg ctccgcgacg ctggcgatcg tcatgtacgg cctgttcgag atcacccttc
      961 agctgatccg catgctgggt cgctgagcgg atggacgctc cggtctggct cctgaccgat
     1021 ccggccatgg atcgccatgc gacgcacgga catccggaac gacccgagcg acgtcccgcc
     1081 gccgcggccg gggtgcgcga cgccgccggc ggtgaccttg ccgagcccgc ggtcatcccg
     1141 gcagccgacg atgcgatcga gcgcatccac ggagccgcgt acgtcgaacg ggtccgggag
     1201 gccgatgcgg ccggcggatc gtggctcgac cccgacacgt acctcgtctc cgggtcgctg
     1261 gaggcggccc gcctcgcggt gggcgcgacg ctgcaggccg ctcgcgcggc agcgaccggg
     1321 gaggcggaga tcgccttcgc cgcggtccgt ccgcccggtc accatgccgc ggcgcggcgc
     1381 gccagtggct tctgcctctt caacaacgtc gccgtggccg ccgccgagct gcgcgcggcc
     1441 ggcctggcgc agcgcatcgc gatcgtggac tgggatgtcc accacggcga cggcacgcag
     1501 gcgatcttcg acgacgatcc ggacctctgc tacgcctcga cccaccagtg gctgctctac
     1561 cccggcaccg gtgaccgcca cgaccgtggc gtcggaccgg cggaggggac gaagcacaac
     1621 gtgccgctgc tgcccggcaa gggcgacgcc gccttcgtca cggcgtggag ggacgagctg
     1681 ctgccggcgg tcgaggcgtt cgggccggag gcgatcctcg tctcggcggg gtacgacggc
     1741 caccacgacg acccgctcgc ccacctggag atcaccgagg acgggtacga gcaggtcgcc
     1801 cgcctcctgg gggcgactgc cgcccggctc ggcctgccgg gcgtcacgct ggccctcgag
     1861 ggcggctacg acctggacgc cctgcggcgg tcggtggccg ccaccgtccg cgggctgcgc
     1921 cacggccggc gctcggcgca cgaggtcgcg cggtgatcga gcgatatact cgacccgggt
     1981 tcacaggagc acgaagcgtg gcacgcagcg cgtcgatcga ggggaaggcg cccgagcagg
     2041 ctggcacgcc ggccgaggcg ggggagaccg ccgtcgacca tgtcctggtc atcagcggcc
     2101 tgtccggcgc cggcaagagc caggcggcga agctgttcga ggacctgggc tacttcgtgg
     2161 tcgacaacct cccgcccgcg ctgctggacg acttcctggc gctgcgtcgc gccgatccgg
     2221 agcggtaccg ccgatccgcc ctcgtcctcg acgtgcgcag cggcgacccg gcgccggcca
     2281 tcgaacgggc cgccagcgcg ctccgccgcg accagggccg ccttgaggtc atctacctgg
     2341 aggccagcga cgcgacgttg atcagccggt tcagcgaaac gcgccaccgc catccgctcg
     2401 agggaacccg cagcggagtg caggccagcc tcgccgagga gcgggagctg ctgaccccca
     2461 ctcgcgagat ggccaaccac gtcatcgaca ccagcggcct gtccatcggc cagctcaagg
     2521 accggctctt cgcgctggtc ccgcgcaccg accaggacga cgcgctgcag atcgacatcg
     2581 tcaccttcgg gttcaagtac ggcatcccgc tcgaggcgga cctggtcttc gacgtgcggt
     2641 tcctgacgaa cccgtactgg gagccgcacc tcaagccgct atccggcctc gaggcgccgg
     2701 ttcgcgactt cgtcctcggc cagcccaagg cgatgcggtt cctggagctc gtgcacgagc
     2761 tgctggagct caccgtgccg gcctaccgcg ccgaggggaa gggccgtctg cggatcgcgc
     2821 tcggttgcac cggcgggtac caccgctcga tcgcgctggc ggaggagctg gccaccagcc
     2881 tgcgtgacct gcccggctca tcggtctccg tcttccaccg ggagctggag cgatgaggct
     2941 cggtcgcctg ccgcgctgga tgtacccggg gatgcacctc aagcgctggc tgctgctgct
     3001 gttcgtcggc atcgccatcc tgggcctcgg cgccgccatc ttcatccggg acctctaccg
     3061 ctcgaccgat gccgacgaga tcgcgatcgt ctactggctg accggcgcct ggcttgagcc
     3121 gcagatccgg gcggcgctgg tggcggcgct cggcgtggtg ctgaccgtca tcggcatgtg
     3181 gggcctcatg cgctcggtcg tctcgccctt cgtcgcccgg ggcgacagcg tgctggaggt
     3241 gctgtacacg aagcgctacc tggcgcgcgg cccgcgcatc gtggccatcg gcggcgggac
     3301 ggggctgtcg accctgctgc gcgggctgaa gggctacagc gcgaacatca cggccgtggt
     3361 ggccgtcgcc gacgacggcg gcagctccgg acagctccgc cagcagctgg ggatcatccc
     3421 gcccggcgac atccgcaact gcatcgcggc cctcgccgac gccgagccat tgatgaccca
     3481 gctgatgcag taccggttcc cggcgggatc gggcctggac aaccacgcct tcggcaacct
     3541 gttcatcgcc gccatgacgg ccgtcaccgg cgacttcgag gaggcggtgc gcgagtccaa
     3601 ccgcgtcctg gccgtccgcg gccaggtgct gccggccacc acggtgccgc tgaacctgtc
     3661 ggcccgcctg cagtccgggc ggatcctggt cggccagtcc ggcatcgccc acgccgagga
     3721 gccgatcgaa caggtgttca tcgagccgaa cgacgtccgc gccaacggcg aggcgctgga
     3781 gcgcatcctg gaggcggaca tggtggtcat cggtccgggc agcctcttca gcagcatcct
     3841 gcccaacctg ctcatcccgg acgtgcgtga tgcgctggcc gccgccccgg gcctgcggat
     3901 ctacgtctgc aacgtggcga cccagccagg cgagacgccc gacctcaccg cctcccagca
     3961 ccttacgacg ctcttcgagc acgtcggcga cgacctgatc gactgcgtca tcgtcaaccg
     4021 cggcagcgct cgtcgacccg agggctggcg cgcgcagccc gtcgaggttg acgtgcggcg
     4081 cctggaggag ctgccggtgg tgatcgtcga ggaggacgtc atcgatcccg ccaacgcgca
     4141 ccgccacgac ccggcgaagc tcgccgccac cctgatgcgc ctccagcagg aggaccgcgt
     4201 cgagcgcccg cgacagcggc gcgtgcgacg cccgacggcc acggcatcct gacgacgatg
     4261 ctcgtcgccg gcgaggtcag gggcgagctc gcccggatcc aacccgcccg tgcctgctgc
     4321 cgccgcgcgg agctccacgg cctgctcgcc ggcagccgcg atggcgtgct gcggaccctg
     4381 gacgtcgcga cggcgcgcac cgccatgcag ctcgcctccg cggtcggcgt cgcgctcccg
     4441 gcgcctcccc gcggcgccgc ttccacgtcg cgccgggcgg gcgggcgcca ccacctggcg
     4501 gtgccactcg acctggacgc gctcggcgca tggtcctgga cagcggcacc ggcctgcgac
     4561 cgtcgctcgt acctgcgcgg ggcgctgctg ggtgcgggct ccatctcgtt cggccgttcc
     4621 ggaacgcacg tcgagttcgt cctcgccgac gacgaggcag gcgaggcgct ccaggcggcg
     4681 cttgcctccg tcgacgtccg cgcgctgcgg tccacgcggc gcggtcgctc cgtcgtctac
     4741 ctcaagggcc aggaggaggt cgccgcgctg ctgcggctcg tcggagccaa ccgcggcctg
     4801 ctggagctcg agacgcaccg ggtgggcgcc gacgtccgtg cgcgactgaa ccgtttgctc
     4861 aacgccgagg aggcgaacct gggccgcacg gtgcgagccg ccgaccgcca gatggcggcg
     4921 atcgcgcggc tcgaggccga cgggacgctg gcgcgcctgg gccacgccct gcgtgacacg
     4981 gcggcgaccc gtcgccgcat gccggacgcc gacctcgata cccttgccgc ggcactcggc
     5041 gtcagccggt ccgccgtgaa ccaccgactg cgacggctcg tggagctcgc cacgggcgag
     5101 gaggactgat gcgcaccccg gtcatcgccg gcaactggaa gatgaacccg cccaccctcg
     5161 aggaggccac cgccctggcg ctcgccatcc gccagctcgc cgacgaactg ccggacgtga
     5221 cgaccgtcgt ctgcccgccg acgatctggc tgcccgacgt cgcgcgcgcg gcccgcggag
     5281 gccggctcca taccggcgcc cagacgatgc acttcgagga gaagggcgcg ttcaccggcg
     5341 agaccagccc gctgatgctg cgtgcgtacg ccgattgcgt gatcctcggc cactccgagc
     5401 gccgccagtt cgattgcgag acggacgagc gcgtcggtcg caaggtggcg agcgccgtcg
     5461 cgcacggcct gcggccgatc gccgcggtgg gggagaccgc cgagcagcga cgggccggcg
     5521 agacggcgga ggtggtcgat cggcaggttc gtgccgcact ggggaacctc gaacgcctgg
     5581 ccggcaccgg actggtcgtc gcctacgagc cggtctgggc gatcggcacc ggggacgccg
     5641 cctcgccgga cgacgcgcag gaggtcgccg cgcagattcg cgccatcctc cgcgagctgg
     5701 actccgccgc ggcggacgac atcccgatcc agtacggcgg cagctgcacg gccgacaacg
     5761 ccccagactt cttcgccatg ccggacatcg acggcgcgct cgtcggcggc gcctccctga
     5821 aggccgacaa cttcggcggg atcctgcggg ctgccgctga cgcggtggcc gcatgacggt
     5881 cgtcctggtc gtcctcgacg gcttcgggat gggggacgac ccgaagcgca gcgccatcct
     5941 cgcggcccgc atgccgaact ggcggcggct cctcgccgag ttcccgaacg cccggctcgc
     6001 ggcctccgga gaggcggtcg gcctcccgcc gggccagatg ggcaacagcg aggtcggcca
     6061 cctcaacctg ggcgccgggt tccgcgtcct ccaggacctc ccgcgcatca acgcggccat
     6121 cgccgacggc agcttcttcg ccaaccccgt cctggtctcg acgtgccgcg gcgtcctcga
     6181 gcgtggtggt cgccttcacc tgatgggcct ggtcggaccc ggcggcatcc acgcggtcga
     6241 cgagcacctg gtggccatgg tggacctggc cgctcgcgtc ggcctgccgg aggatcgggt
     6301 gctgttccac gccttcaccg acggccgtga cacccctccg cggtccgccg accgattcct
     6361 gcccgagctc gaagctcgcg ttgctggccg tgctcgagtg gccacggtca ccggccgcta
     6421 ctgggccatg gaccgcgaca ggcgctggga ccggacgaag cgcgcgtacg acgccatcgt
     6481 ccatgggcag gggctccggg cggtggatgc caccgcggcg atcgcggtcg cctacgcccg
     6541 cgacgagggc gacgagttca tccagccgac cgtgctcgac gccgcggtcc cgatgaccga
     6601 cggcgacgcc gcgatccacc tcaacttccg cgcggatcgg gcacggcagc tgaccgccgc
     6661 gctcaccctc gacgagttcg acgggttcga ccgaggtggt cggcgccccg acgtcgaggt
     6721 ggcgacgctg accgagtacc aggcgccgga cgagctgccg gttcgggtcg ccttcccgcc
     6781 gcttcagatc gactccctgg ccgcgcacct cgaccgcctg ggcctgaccc agctgcacgt
     6841 cgcagagacg gagaagtacg cccacgtgac ctacttcttc aacggcggcg tcgaggatcc
     6901 cttcgctggc gaggaccgca tcctgatccc gtccaaccgc gcggtcgcga cctacgatca
     6961 ggcgccggag atgagcgcgg cgccaatcac cgatgcgatc gtcgagggca tcgaaggtgg
     7021 ggtgtacacc ttcctggtcg cgaactacgc caatccggac atggtcgggc acaccggcga
     7081 ctgggacgcg accgtatcgg cgctggagta tctcgacgga tgcctggggc gcgtcgcgga
     7141 cgcagccctg cgaacatcca gcccgctgat gatcaccgct gatcacggca acgcggagga
     7201 gatgcgcgat ccctccggcg ctccgcagac gaagcacacg acgtcgccgg tgcccgtggt
     7261 gctggtcagc gccgcgcacg gcgggcgcac gctccacgat ggggagctgc gggacgtcgc
     7321 cccgacgctg tgcgagctgc tcggcatccc accggggccg gagatgacgg ggcacagcct
     7381 gctcgatcgg tagcggaggg cggggtcgta tatcggtata tacgccgcgc cgaacacagg
     7441 gaccctgcta gactccggcg gtcatgcaga cccctttgct catcgcccag atgatcctgg
     7501 ccgtggcgct gatggcggcg atcctgctcc agcagcgcgg caccggcctg ggcggcgcct
     7561 tcggaggcga ggtcacggcc taccggagcc ggcgcgggat cgagcggacg ctgttccgcc
     7621 tgaccatcgt gctcgccgtc ctgttcgtcg cgttcagcct gctcaacctc ttccagcagg
     7681 gctgaaaggc ggggccgcgt atcggcctgt gctatgatcc aatcgcgcat tcggatccgt
     7741 ccggatgcgt tcgtgccgag gtggcggaat aggcagacgc gctaggttca ggacctagtg
     7801 accgcaaggt cgtgtgggtt caagtccctc cctcggtacc accctcgatg cccaggtggc
     7861 ggaattggta tacgcgtacg tttgaggggc gtatggggaa acccatccgg gttcaagtcc
     7921 cggcctgggc accaattcag atctgatccc cgcaccgatg cgccgctcgc tcc
//
LOCUS       DASTYP010000027         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_766766, whole genome shotgun sequence.
ACCESSION   DASTYP010000027 DASTYP010000000
VERSION     DASTYP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766766"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFV59_01335"
     CDS             <1..705
                     /locus_tag="VFV59_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5324503.1"
                     /translation="AANMVLNLAAFIAFERISIALTLLVFYLYPAGVALASTMWFGER
                     LDRLRWGALVVSLAGMALVVAGAESLGSVDPVGIGLAFLAAMAQVFYVLAARHGFAHV
                     PGPQAAALTMGGAATLYVLVAFGIGTVGALGQPVASSTAFVPVLLAGVIGAGIPTLAF
                     ILGIRRLGPSQAAILSTLEPVVGTALAAWLLAETPTGVQLVGGVLILGAAVLLQLRRG
                     PRAEHEALDMDAEETA"
     gene            complement(837..1985)
                     /locus_tag="VFV59_01340"
     CDS             complement(837..1985)
                     /locus_tag="VFV59_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324504.1"
                     /translation="MTDAREQVAQLLTTALLRDLGDEVDLVFRYGSTVRGGGTRTHRF
                     SDVDISYVPVHEDTSRALTVMVATTMCDLYPIRWSKLERMAELDDPSASILLDAVVVH
                     HRTDAAAERFARLGARLRENLAPPARARMLTRAHEIFMRTGYPYYLLRSAEQRDHRFA
                     ALFHARQVVGGVLQAVAVANQRCVDTRRLSDVLELDRLPERFSSLLDAAIRASAPAEA
                     LGACEALMASSREFLLAEQREVHRGEASFADALDAAYPELMGDLQHAVIAAEAEDQWR
                     LTVVPMLHELMVAVAEARTGVAHSSFNSPPEYEQDLEALGFPDLLSLAAAHDFEGIRR
                     VAPVFADRLRSYLVENGAALNAFDSVDELRAFLGLPEDGSGTEAPGRD"
     gene            complement(1989..>2987)
                     /locus_tag="VFV59_01345"
     CDS             complement(1989..>2987)
                     /locus_tag="VFV59_01345"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU5324505.1"
                     /translation="DKFGGSVLRRPEDIAAAVDVVARQRAAGLRPVVVVSAFEGVTDT
                     ILGAARVLLPDEGAELRRPGHDADSPLARETDRALATGEALSAALFALGLQARGIPAR
                     SFSGAEAGIRTAAAHLGAEVRRVLSRPLRTALNDGLVPVVAGFQGIGTHGELTTLGRG
                     GTDLSAVVLAIALRAARCELFKDVGGVLEADPHLVPAARFLPAVDALQLELLAELGSE
                     VVHPVAVRRARRGRLHLVVRALEDDAPQTVVRPSTAADDGVLMLAVDRKERMARVSLV
                     GPAALTPEVLPLPAHGEGSFDEGGMRVVWAEVPVASAQSAAQAVHASFMGRRRSEG"
BASE COUNT          400 a         1114 c         1091 g          382 t
ORIGIN      
        1 gcggccaaca tggtcctgaa cctcgcggcg ttcatcgcct tcgagcgcat ctcgatcgcc
       61 ctgaccctgc tcgtcttcta cctctacccc gccggcgtgg cgctcgcctc gacgatgtgg
      121 ttcggcgagc ggctggaccg gctgcgctgg ggcgccctgg tggtctcgct ggccggcatg
      181 gcactcgtcg tcgcgggcgc ggagtcgctg gggtcggtgg acccggtcgg catcgggctg
      241 gcgttcctgg ccgccatggc ccaggtcttc tacgtcctgg cggcgcgcca cggcttcgcc
      301 cacgtccccg ggccgcaggc cgcggccctg accatgggcg gtgcggcgac cctctacgtc
      361 ctggtcgcgt tcgggatcgg aaccgtcggc gcgctcgggc agccggtcgc gtcgagtacg
      421 gcgttcgttc ccgtcctgct ggccggcgtc atcggggccg gcatcccgac cctcgccttc
      481 atcctgggca tccggcgcct tggtccctcg caggcggcca tcctctcgac cctcgaaccg
      541 gtcgtgggca cggcgctggc cgcgtggctg ctggccgaga cgccgaccgg ggtgcagctg
      601 gtgggcggcg tcctgatcct cggggcggcg gtgctgctcc agctgcggcg gggtccccgt
      661 gccgagcacg aggcgctcga catggacgcc gaggagacgg cgtagccctc gctccggcac
      721 ggcgatggct cggatgcatc acagcgcgcg cgaggctgct ttcgcgcgtc ggccgatggg
      781 tgggacggat caggggacgc gcgaacgccg cgtccggggc gctgtgatca accccgtcaa
      841 tcgcgcccgg gagcttcggt ccccgagccg tcctcgggca gtcccaggaa cgcccgcagc
      901 tcgtcgacgg agtcgaaggc attgagggcc gccccgttct cgacgaggta ggaacgcagc
      961 cgatccgcga acaccggcgc gacccgccga atgccctcga agtcgtgggc ggcggcgagc
     1021 gagagcagat ccgggaaccc gagcgcctcg aggtcctgct cgtactccgg tggcgagttg
     1081 aacgacgagt gggcgacacc ggtgcgcgcc tccgcgaccg ccaccatcag ctcgtggagc
     1141 atcgggacga ccgtcagccg ccactggtcc tcggcctcgg cggcgatcac cgcgtgctgg
     1201 aggtcgccca tcagctcggg ataggcagcg tccagcgcgt cggcgaagct cgcctcgccc
     1261 cggtggacct cgcgctgctc ggccagcagg aactcgcgcg acgacgccat cagcgcctca
     1321 caggccccga gcgcctccgc cggcgccgag gcgcggatcg cggcgtccag gagcgacgag
     1381 aagcgctccg gcaggcggtc cagctcgagc acgtcggaca ggcggcgcgt gtcgacgcag
     1441 cgctggttgg ccacggcgac ggcctggagg acgccgccga cgacctgccg ggcatggaac
     1501 agtgcggcga agcggtgatc gcgctgctcg gctgagcgca ggaggtagta ggggtagccg
     1561 gtccgcatga agatctcgtg ggcgcgggtc agcatgcgcg ctcgcgctgg aggcgccagg
     1621 ttctcgcgca ggcgggcgcc gaggcgcgcg aagcgctcgg cggccgcatc ggtccggtgg
     1681 tggacgacca cggcgtcgag caggatcgag gcgctcggat cgtcgagctc ggccatgcgc
     1741 tcgagcttcg accagcggat cgggtagagg tcgcacatcg tcgtggcgac catcaccgtc
     1801 agcgcgcgcg acgtgtcctc gtgcaccgga acgtagctga tgtcgacgtc actgaaccga
     1861 tgcgtgcgcg tgccgccccc tcgcaccgtc gagccgtagc ggaagaccag gtcgacctcg
     1921 tccccgaggt cccgcagcag cgcggtggtg aggagctgcg ccacctgctc ccgagcgtcg
     1981 gtcacgactc agccctcgct gcggcgacgt cccatgaagg aggcgtgcac cgcctgcgcc
     2041 gcggactggg ccgatgcgac gggcacctcg gcccacacga cccgcatacc cccctcgtcg
     2101 aacgaaccct cgccgtgagc ggggagcggc aggacctccg gcgtgagggc ggccggcccg
     2161 accaggctca cccgcgccat ccgctccttg cggtccacgg cgagcatcag cacgccgtcg
     2221 tcggccgcgg tggagggacg gacgacggtc tgcggcgcgt cgtcctccag ggcgcgcacc
     2281 accaggtgca gccgcccgcg acgcgcccgc cgcaccgcca cggggtgcac gacctccgac
     2341 ccgagctcgg ccagcagctc cagctggaga gcatcgacgg cgggcaggaa gcgcgcggcc
     2401 ggcacgagat gcggatcggc ctcgaggacg ccgcccacgt ccttgaacag ctcgcagcgc
     2461 gcggcgcgca gggcgatcgc cagcaccacg gccgacaggt ccgtgccgcc ccggcccagc
     2521 gtggtcagct cgccgtgcgt cccgatcccc tggaacccgg cgaccaccgg gaccagcccg
     2581 tcgttcaatg cggtccgcag cggccgcgag aggacgcgcc ggacctcggc ccccaggtgc
     2641 gcggccgcgg tccggatccc ggcctcggca cccgagaacg aacgcgccgg gatgccgcgc
     2701 gcctgcaggc cgagcgcgaa gagcgcggcc gagagcgcct cgcccgtcgc cagggcgcgg
     2761 tccgtctccc gggccagcgg cgagtcggcg tcatgacccg gccggcgcag ctcggctccc
     2821 tcgtcaggca gcagcacgcg ggccgccccc aggatcgtgt cggtgacgcc ctcgaaggcg
     2881 ctgaccacca ccaccgggcg cagcccggcg gcgcgctgac gcgccacgac gtccacggcc
     2941 gcggcgatgt cctccggccg ccgcaggacg gagccaccga acttgtc
//
LOCUS       DASTYP010000028         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_365964, whole genome shotgun sequence.
ACCESSION   DASTYP010000028 DASTYP010000000
VERSION     DASTYP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365964"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFV59_01350"
     CDS             complement(<1..530)
                     /locus_tag="VFV59_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324506.1"
                     /translation="MSRTIELAAGLVLLVIGVVVLASEPIIGWISGTGVGDEILGWWP
                     ILVVALSLFFLVPAVIGPQSRRLRAGMVIPGALLAGIGGALLYTSLTDRWGAWSSLWA
                     VLPFSFGLGMYAAGWIADAPAFKWIGAGVAIGGVVAYLVFATAFGGEAFRLIAALGIL
                     ALGVALTIGGLAERLSR"
     gene            563..2878
                     /locus_tag="VFV59_01355"
     CDS             563..2878
                     /locus_tag="VFV59_01355"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5324507.1"
                     /translation="MVARPGRRSPLRLLGRRLPPTRGIWRVPGIRAQVSIGRDAHGVA
                     YVEASSAEDAWYGLGFCHGQDRGFQLEILLRAGRGTVAELLGPDALPIDRLTRTLGFA
                     RVAREQLPVLDPDIRASVTAYAAGINAAASVSPRPHELAMLRATRTPWTAEDCLAFMG
                     LQSLALAGNWDSELARVQILLADGPDALARVDPTYGPWLPAVIPVGSAVGEPIGRLAA
                     DLTRLRDLVGGPAASNAWAVAGARTATGAPILANDPHLAPTVPGPWYLAQLRTPEWAV
                     AGCSFVGGPAFPTGHNGRLAWGITAGCTDSADLFWEQIDPDAGTARGPQGPEPIDVIR
                     QEIPVRGAAAEPLEIVVTRRGPVVTPALPGLDVALSLRATWLEPVPVRGLLDVVGARD
                     VGAFRSAFAAWPGPALNVVFADAGGHIGWQLIGTLPRRRAGNGTLPLPAWDGELGWEP
                     EHLPFDAMPSCVDPDAGFVASANHAARTDDPDQPFLGVDWLDGYRAQRIVEVLQARAD
                     WDVASTQALQVDTTSVPWRELRDLLLAGDAHDDRARTARELLAAWDGDVAAGSVAASV
                     FELTVLELATAVAQEAAPTGWEWVLGRGFGMAAPRTSFGARIVSQLVATLRGHPDPRP
                     MVDAALAAAVGTLESVAGPDASGWAWGSVRPLRLLHTFGAQRALAAIFNVGPVPLGGD
                     TNTVPQAGVLPLEPFANPAAIATHRTVIDLGDLEASRYVIAGGQSGNPLSRHYDDLFA
                     RWRRGEGVPIAWSRDAVDAAVRQRLLLVPEA"
     gene            complement(2890..>3836)
                     /locus_tag="VFV59_01360"
     CDS             complement(2890..>3836)
                     /locus_tag="VFV59_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5324508.1"
                     /translation="RVILSVHTGLNSLTLLQWGSEEQKQQYLVPQARGEKLATFGLTE
                     PGVGSDAANLATTARRDGDRYILNGSKIWISLADTADHFLVFATVDRALGHKGITAFI
                     VERSFPGFSSDSLHGKLGVRAGNTGILYFNDCEVPAANRVGEEGEGFTIAMSAIDQGR
                     YTVAAGSVGLAQACLDASVKYAHERQAFGEEIGRHQLVKQMIAKMHQGIEAGRLLVWK
                     AGWKKNRGERNTQETSLAKWFATDHAVASALDAIQIHGAYGYSNEYPVERYLRNSKGS
                     VIYEGTSQIHTLMQADYLLGYRKDKPLRMPQLPFEGEM"
BASE COUNT          495 a         1422 c         1370 g          549 t
ORIGIN      
        1 cggctgagcc gctccgccag cccaccgatg gtcagggcca cgcccagcgc caggatgccg
       61 agcgcggcga tgagccggaa ggcctcgccg ccgaaggcgg tggcgaacac caggtaggcg
      121 accacgccac cgatggcgac gccggcgccg atccacttga acgccggcgc atcggcgatc
      181 catccggccg cgtacatgcc cagcccgaag ctgaagggca gcacggccca cagcgagctc
      241 cacgcgcccc atcggtccgt caggctcgtg tacagcagcg cgccgccgat gcccgccagg
      301 agcgcgcccg ggatgaccat ccccgcccgc aaccggcgtg actgcggacc gatgacggcc
      361 gggaccagga agaagagcga gagggcgacg accaggatcg gccaccaccc caggatctcg
      421 tcgccgacgc cggtgccgct gatccagccg atgatgggct cgctggccag caccacgacg
      481 ccgatgacca ggaggaccag gccggcagcc agctcgatcg tgcgactcac ggcggggagt
      541 atacggtggc atcatcggct ccatggttgc ccgtcccgga cgccgatcgc ccctgcgtct
      601 gctcggccgt cgcctgccgc cgacgcgcgg catctggcgc gtgccgggca tccgcgccca
      661 ggtctcgatc ggtcgtgacg ctcacggcgt cgcgtacgtg gaggcgtcct cggccgagga
      721 tgcctggtac ggcctcggct tctgccacgg gcaggaccgc ggcttccagc tcgagatcct
      781 gctgcgggcc ggacgcggga cggtggccga gctgctcggt cccgacgcgc tgccgatcga
      841 tcggctgacc cgcaccctcg gcttcgcccg cgtcgcccgg gaacagctgc cggtcctcga
      901 ccccgacatc cgcgcctcgg tgacggccta cgcggccggc atcaacgccg ctgcttccgt
      961 ctcgccacgc ccgcacgagc tcgccatgct gcgcgccacg cgcacgccgt ggacggcgga
     1021 ggactgtctc gccttcatgg gcctccagtc cctggccctg gccgggaact gggactccga
     1081 gctggcgcgg gtgcagatcc tcctggcgga cggccccgat gcgctggcgc gcgtcgaccc
     1141 gacctacggc ccgtggctgc ccgccgtcat ccccgtgggg tcggcggtcg gtgagcccat
     1201 cggccggctg gcagcggacc tcacgcgcct gcgagacctc gtcggcggcc cggccgcgtc
     1261 gaacgcctgg gccgttgcgg gcgcgcgcac cgcgaccggc gccccgatcc tggccaacga
     1321 cccgcacctg gcacccacgg ttcccggtcc ctggtacctg gcccagctgc ggacgcccga
     1381 gtgggccgtg gccggctgct cgttcgtcgg cgggcccgcg ttcccgaccg ggcacaacgg
     1441 tcgcctggcg tggggcatca ccgcgggctg cacggactcg gccgacctgt tctgggaaca
     1501 gatcgacccg gacgccggga cggcacgcgg tccacagggg ccggagccga tcgacgtcat
     1561 ccggcaggag atcccggtgc gtggcgccgc cgcggagccg ctggagatcg tcgtgacacg
     1621 ccgcggtccg gtggtgactc cggcgctgcc tgggctcgac gtcgcactct ccctgcgcgc
     1681 cacgtggctc gagccggttc cggtccgcgg cctgctggac gtggtcggcg cccgggacgt
     1741 gggcgcgttc cgcagcgcct tcgccgcgtg gcccgggccg gcgctgaacg tcgtcttcgc
     1801 cgatgccggc ggacacatcg gctggcagct catcgggacg ctgccacggc ggcgcgcggg
     1861 caacggcaca ctgccgctcc ccgcctggga cggcgagctc ggctgggagc ccgagcacct
     1921 gcccttcgac gccatgccgt cgtgcgttga cccggacgcc ggcttcgtcg cctcagcgaa
     1981 ccatgcggcg cggacggacg accccgatca gcccttcctc ggcgtcgact ggctcgacgg
     2041 ctaccgagcg cagcggatcg tcgaggtgct gcaggcccga gccgactggg acgtcgcctc
     2101 gacgcaggcg ctccaggtgg acacgacctc ggtcccgtgg cgcgagctgc gggacctcct
     2161 gctcgccggc gacgcgcacg acgaccgcgc ccggacggcg cgcgagctgc tggcagcgtg
     2221 ggacggcgac gtcgcggccg gctcggtggc cgcatcggtc ttcgagctga cggtgctcga
     2281 gcttgcgacc gccgtcgcgc aagaggcggc gccgaccggc tgggagtggg tgctcgggcg
     2341 cgggttcggc atggccgcgc cgaggacgtc gttcggcgcg cggatcgtga gccagctcgt
     2401 cgcgacgctg cgcgggcacc ccgacccgcg tccgatggtg gacgcggccc tcgccgccgc
     2461 ggtcggcacg ctggagtcgg tcgccggacc ggacgcgagc ggctgggcgt ggggatcggt
     2521 ccgcccgctc cgccttctcc acacgttcgg cgcgcagcgc gcgctggctg ccatcttcaa
     2581 cgtcggcccg gtcccgctcg gtggcgacac gaacacggtt ccgcaggccg gcgtcctgcc
     2641 gctggagccg ttcgctaacc cggccgccat cgccacgcac cgcacggtca tcgacctggg
     2701 cgacctcgag gcgtcgcgct acgtgatcgc cggcggccag tccggcaacc cgctgtcacg
     2761 ccactacgac gacctgttcg cgcggtggcg ccgcggcgag ggcgtgccca tcgcctggtc
     2821 gcgcgacgcc gtggatgccg cggtgcgcca gcgcctgctc ctcgtccccg aggcctgagc
     2881 cgccgggagc tacatctccc cctcgaaggg cagctgcggc atccgtagcg gcttgtcctt
     2941 gcggtacccg agcaggtagt cggcctgcat cagggtgtgg atctgcgacg tgccctcgta
     3001 gatgaccgag cccttggagt tgcgcaggta gcgctcgacg ggatactcgt tcgagtagcc
     3061 gtaggcgccg tggatctgga tcgcgtccag cgcggaggcg acggcgtgat cggtggcgaa
     3121 ccacttcgcc agcgaggtct cctgggtgtt ccgctcgccg cggttcttct tccagccggc
     3181 cttccagacc agcaaccgac ccgcctcgat gccctggtgc atcttggcga tcatctgctt
     3241 gaccagctgg tgccggccga tctcctcacc gaaggcctgg cgctcgtgcg cgtacttgac
     3301 cgatgcgtcc aggcacgcct gcgccagccc gaccgatccg gccgccacgg tgtagcggcc
     3361 ctggtcgatg gcgctcatgg cgatggtgaa gccctccccc tcctcgccga cccggttcgc
     3421 cgcagggacc tcgcagtcgt tgaagtacag gatgcccgtg ttgcccgcac ggacgccgag
     3481 cttgccgtgg agcgaatcgg agctgaagcc cgggaacgag cgctcgacga tgaaggcggt
     3541 gatgcccttg tggccgaggg cgcgatcgac ggtcgcgaag accaggaagt ggtcggccgt
     3601 atcggccagg ctgatccaga tcttgctgcc gttgaggatg tagcgatcgc cgtcgcgccg
     3661 ggccgtcgtt gccaggttgg ccgcatcgga cccgacgccc ggctcggtca ggccgaacgt
     3721 ggcgagcttc tcgccacggg cctgtggcac gaggtactgc tgcttctgct cctcgctccc
     3781 ccactggagc agggtcaggg agttgaggcc ggtgtggacg ctgaggatca cgcgga
//
LOCUS       DASTYP010000029         5925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1495335, whole genome shotgun sequence.
ACCESSION   DASTYP010000029 DASTYP010000000
VERSION     DASTYP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495335"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /gene="uvrB"
                     /locus_tag="VFV59_01365"
     CDS             complement(<1..1820)
                     /gene="uvrB"
                     /locus_tag="VFV59_01365"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU5324509.1"
                     /translation="MPDLKVNAPYEPTGDQPQAIAALADGVEKGLKHQVLLGATGTGK
                     TFAAAKLIEAVQKPTLVMAHNKTLAAQLYQEFKEFFPDNAVEYFVSYYDYFQPEAYLP
                     RTDTYIEKDSSRNEEIDRLRHNATRALFERRDVVIVASVSAIYGLGAPVDYGATVINL
                     KRGGRYRRDGILRHLVDLQYQRNDAALGRAKFRVRGDVLEVQPPHGEYVVRIDFFGDE
                     VERIVEIDDLTGEVLAERTEVNLYPATHYVTPRDKLLAAVEKIEDEMEERVRWLADQG
                     RELEAARLRQRTQFDLEMMREVGYCSGIENYSRHLAGREAGSRPWTLIDYFPTDFLLI
                     VDESHITIPQVHGMYKNDRTRKEILVDFGFRLPSALDNRPLTFEEFEDHMSQVVYMSA
                     TPGPYETERAERVAEQFIRPTGVVDPQITVRPTSGQIDDLLAEIRATVERGERALVTT
                     LTKKMAEDLADYLAELGIKVQWLHSEVDTLERVEILRDLRLGVYDVLVGINLLREGLD
                     LPEVTLVAILDADKEGFLRSGGSLIQVIGRAARNVGGRVIMYADRVTDSMKAAIDETD
                     RRRAKQVAYNEEHGITPETVMKEIRDIHEGLRRVAEERAKV"
     gene            1996..2589
                     /locus_tag="VFV59_01370"
     CDS             1996..2589
                     /locus_tag="VFV59_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEU5324510.1"
                     /translation="MPAVPAGRPRPAERGQAMVEFAAVLLPVLLLIVGIIQFGLIFGA
                     NVTLTNAAREAARAATIERFDTTVSRSVNDQARCTAALGAAVQSFGLMNAASPNFVAS
                     SSCPSGGASDLNGDGLADRWVNGDLTMTLCSSMATPTSPCPTTGTYCGLTDPTGCLVR
                     VTLTYRSDIIVPFIGDILSTDAGGRFVQTASATMVVN"
     gene            2592..4562
                     /locus_tag="VFV59_01375"
     CDS             2592..4562
                     /locus_tag="VFV59_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEU5324511.1"
                     /translation="MATPAPRGQVLVLFAILITILLAFAGITVDIGRQVAERRHVQTA
                     ADAGALAGCRSLIQGDSDATAASVARSVALANLGHSPAGAAATIADPPQYEDEDGNGV
                     IEAHELTSGVVIAGTTVRVAISSVVDTTLARVVGVSTLETGARARCDLQGSPALPIVA
                     RRYASPPGPGSGFVDHLASAATSVNGVVDTSNPRGYDVRTPASEANPGPNFSIYGPSS
                     KANNDSSFRGFVALDVRNFEGLDTRVYYNGVTAGTTDNTLKDIEGAYLVEGYPGPAFP
                     PVSVPPNGTTQVGVLSGNSTSFVVNKFDDTFRVGDRILLGVYDGTVMQIPDFSLTPPA
                     EILLPGTTTAPVDGPSVTVSRNDQFLSTVTLSLGGDIGAAAAGMPEANILPDPPVTPP
                     PSGFMTEPTWSQNVFQPARSGTSVAMEDFQTLDVPAGVYTVWLEGESGNPYYQRRSVP
                     LPVRIQTDANGDGDYNDAGDVKVTRDFSLVNSVLDGSTPTVGGTITLPIRVSTSGQQW
                     DGGPSLETAVSLSWDTSSLTDCSLNPASLASGSIGFSATSVSPGGGQGTLSSLTISTA
                     GMSQGCYLFTIRAHGINGDGQPITRLQRVRFTVATTTSNGQYVDIIGFAVFHVDTVIS
                     NEITGHAVSPISADPGDPALRRAQRPRLVPWN"
     gene            4589..5410
                     /locus_tag="VFV59_01380"
     CDS             4589..5410
                     /locus_tag="VFV59_01380"
                     /inference="COORDINATES: protein motif:HMM:NF028286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HEU5324512.1"
                     /translation="MELEYSDRNSRRSKIYIAVGVIVALLVAATVFLALQASGLTQDR
                     AVEMRDVVVATRDIPARKAIEEGDVQVRSVPVDPTNDTAFASLDEVLGRVVGIPVATG
                     QLVTRNVLASTTEGQTFSILEPGVEFDPEGPHLRAVSVTVAPANAVAGTLVAGQRVDL
                     IATMAINPELGQTPEEAEAQSAQFLPGPSTKVTLQSMTILARNGDIYILRSDVETAEK
                     IAELTAAGATFTMVLRPDQDDRTAETDGSTIDMLIDEFDFPVPVPPQFEERRAGN"
     gene            complement(5407..>5925)
                     /gene="coaE"
                     /locus_tag="VFV59_01385"
     CDS             complement(5407..>5925)
                     /gene="coaE"
                     /locus_tag="VFV59_01385"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5324513.1"
                     /translation="GMLREHGVATIDADVVAREIRHNDADARRAIEARFGTYEAAELG
                     RIVFADPNALADLEAILHPRIRGAIRARLAELAEGNVEVAAVEVIKLFESPLADACDE
                     LWVVRCDEADAIARLAASRGMDEAAARRRLASQSSQEEKVARADVVIDGSAPLEETRR
                     EVESALERLRAR"
BASE COUNT          863 a         2183 c         1964 g          915 t
ORIGIN      
        1 accttcgcgc gctcctcggc gacgcgacgc aggccctcgt ggatgtcgcg gatctccttc
       61 atcaccgtct cgggggtgat gccgtgctcc tcgttgtacg cgacctgctt ggcgcgccgg
      121 cggtcggtct cgtcgatcgc cgccttcatc gaatcggtca cgcggtccgc gtacatgatc
      181 acgcggccac ccacgttgcg ggccgcccgg ccgatgacct ggatgaggga cccgccggag
      241 cgcaggaagc cctccttgtc ggcgtccagg atggccacca gcgtcacctc gggcaggtcg
      301 agtccttcgc gcagcaggtt gatccccacc aggacgtcgt acaccccgag ccggaggtca
      361 cgcaggatct ccacgcgctc cagggtgtcg acctccgagt gcagccactg gaccttgatc
      421 ccgagctcgg ccaggtagtc cgcgagatcc tcggccatct tcttggtgag cgtcgtgacc
      481 agcgcgcgct cgccgcgctc gacggtggcg cggatctcag ccagcaggtc gtcgatctgg
      541 ccgctcgtcg gccgcaccgt gatctgggga tccaccacgc cggtcggccg gatgaactgc
      601 tccgccacgc gctcggcgcg ctcggtctcg tacggtccgg gcgtcgccga catgtagacc
      661 acctggctca tgtggtcctc gaactcctcg aacgtcagcg gacggttgtc gagggccgat
      721 ggcagccgga acccgaagtc gaccaggatc tccttgcggg tccggtcgtt cttgtacatg
      781 ccgtggacct gcgggatggt gatgtggctc tcgtcgacga tcaggaggaa gtcggtcggg
      841 aagtagtcga tcagcgtcca aggccggctc ccggcctcgc gtccggccag gtggcgtgag
      901 tagttctcga tgccggagca gtacccgacc tcgcgcatca tctcgaggtc gaattgcgtg
      961 cgctgccgga gccgcgcggc ctccagctcg cggccctggt cggccagcca gcggacccgc
     1021 tcctccatct cgtcctcgat cttctccacg gcggcgagca gcttgtcgcg cggcgtgacg
     1081 tagtgggtcg cgggatacag gttcacctcg gtgcgctcgg ccagcacctc gccggtcagg
     1141 tcgtcgatct cgacgatgcg ctccacctcg tcgccgaaga agtcgatccg caccacgtac
     1201 tcgccgtgcg gcggctgcac ctccaggacg tcgccgcgca cccggaactt ggcgcgtccc
     1261 agcgccgcgt cgttccgctg gtactgcagg tcgaccaggt gccgcaggat gccgtcacgc
     1321 cgataccgcc cgccgcgctt caggttgatc acggtggcgc cgtagtcgac cggcgccccc
     1381 aggccgtaga tggccgatac cgaggcgacg atcaccacgt cgcgtcgctc gaagagcgcc
     1441 cgcgtggcgt tgtgccgcag gcggtcgatt tcctcgttgc gtgacgagtc cttctcgatg
     1501 tacgtatcgg tccgggggag ataggcctcc ggctggaagt agtcgtagta gctgacgaag
     1561 tactcgaccg cgttgtccgg gaagaactcc ttgaactcct ggtacagctg cgccgccagc
     1621 gtcttgttgt gagccatgac cagcgtcggc ttctgcaccg cctcgatcag cttcgcggcc
     1681 gcgaacgtct tcccggtgcc cgtcgcgccc aacaggacct ggtgcttgag gcccttctcc
     1741 acgccatcgg ccagcgcggc gatcgcctgc ggctgatcgc cggtgggttc gtagggggcg
     1801 ttgaccttca ggtcgggcat ggggactcga ttctagccgc tccatcggtc ccatcctcga
     1861 ccgcccccaa gggcctatat cggtaccagc gggccctcca taggatcggg ttgctgccac
     1921 ccgaacgtcc gcaacccgca agtcgagccc gtgcccttcg tgatccgtca catccggggg
     1981 atccaccctg caccgatgcc ggccgtcccg gccggccggc cgcgcccggc ggagcgaggg
     2041 caggccatgg tggagttcgc ggccgtgctc ctgccggtcc tgctgctcat cgtcgggatc
     2101 atccagttcg gcctgatctt cggcgccaac gtgaccctca ccaacgcggc tcgcgaggcg
     2161 gcgcgggcgg cgaccatcga acgcttcgac accaccgtct cgcgatcggt caacgaccag
     2221 gcgcggtgca ccgccgccct cggcgccgcg gtccagtcct tcggcctcat gaacgccgcc
     2281 tcgcccaact tcgtcgcgtc gagctcgtgc ccgtccggcg gggccagcga cctcaacggc
     2341 gacgggctgg cggaccgatg ggtgaacggc gacctgacca tgacgctgtg ctcgtcgatg
     2401 gcgacgccca ccagcccgtg ccccaccacc ggcacctact gcggcctgac cgatccgacg
     2461 gggtgcctgg tgcgggtcac cctcacctat cggtcggaca tcatcgtccc gttcatcggc
     2521 gacatcctct cgaccgatgc cggcggccgc ttcgtgcaga ccgcgtcggc cacgatggtg
     2581 gtcaactgac catggccacg cccgccccgc gcggacaggt cctggtcctg ttcgcgatcc
     2641 tgatcacgat cctgctggcc ttcgccggga tcaccgtcga catcggccgg caggtcgccg
     2701 agcggcgcca cgtccagacc gcggcggacg cgggcgcgct ggcgggctgc cgctccctga
     2761 tccaggggga cagcgacgcg acggcggcct ccgtcgcgcg ctccgtggcc ctcgccaacc
     2821 tgggccactc cccggccggc gcggcggcga ccatcgcgga cccgccgcag tacgaggacg
     2881 aggacggcaa cggtgtcatc gaggcgcacg agctgacgag cggcgtcgtc atcgccggca
     2941 cgaccgtgcg ggtcgccatc tcctcggtgg tcgacacgac cctcgcccgc gtggtcggcg
     3001 tctccacgct cgagacgggt gcgcgcgctc gctgcgacct ccagggcagc ccggccctgc
     3061 cgatcgtggc gcgccgctac gcgagtcccc cggggcctgg cagcggcttc gtggaccacc
     3121 tcgcctccgc ggcgacgagc gtcaacggcg tcgtcgatac ctcgaacccg cgcggctacg
     3181 acgtgcggac gccagccagc gaggccaacc cggggccgaa cttctcgatc tacggaccct
     3241 cgagcaaggc caacaacgac tcgagcttcc gtggcttcgt ggcgctcgac gtcaggaact
     3301 tcgagggcct ggacacgcgc gtctactaca acggcgtcac ggcgggcacg accgacaaca
     3361 ccctcaagga catcgagggc gcatacctgg tggaggggta cccgggtccc gcgttcccgc
     3421 ccgtgagcgt tccgcccaac ggcacgaccc aggtcggcgt cctgtccggc aactcgacga
     3481 gcttcgtcgt caacaagttc gacgacacct tccgcgtcgg tgaccgcatc ctcctgggcg
     3541 tctacgacgg gacggtgatg cagatccccg acttctcgct gacgccgccc gccgagatcc
     3601 tgctgccggg gaccacgacc gccccggtcg acgggccgtc cgtcacggtg tcgcgcaacg
     3661 accagttcct cagcacggtc acgctgtcgc tgggtggcga catcggtgcc gcggcggctg
     3721 gcatgccgga ggcgaacatc cttcccgatc cgccggtcac gccgccgccg agcggcttca
     3781 tgaccgagcc cacctggtcc cagaacgtgt tccagcccgc caggtcggga accagcgtgg
     3841 ccatggagga cttccagacg ctcgacgtgc cggccggcgt gtacacggtc tggctggagg
     3901 gtgagtcggg caacccgtac taccagcggc gcagcgtccc gctgccggtc cgcatccaga
     3961 ccgatgcgaa cggcgacggc gactacaacg acgccggcga tgtgaaggtc acgcgcgact
     4021 tcagcctcgt caactccgtg ctggacggtt ccacgccgac cgtcggggga acgatcaccc
     4081 tgccgatccg ggtgagcacc agcgggcagc agtgggacgg cgggccgagc ctcgagacgg
     4141 cagtctcgct gagctgggac acgagctccc tgaccgactg ctcgctcaac cccgcctcgc
     4201 tcgccagcgg ctcgatcggg ttcagcgcca cgtcggtgag cccgggcggc ggccagggca
     4261 ccctgtcaag cctcaccatc agcacggccg gcatgtccca gggctgctac ctgttcacga
     4321 ttcgcgccca cggcatcaac ggggacggcc agccgatcac ccgtctccag cgcgtgcgat
     4381 tcaccgtggc caccaccacg agcaacggcc agtacgtcga catcatcggc ttcgccgtct
     4441 tccacgtgga cacggtcatc tcgaacgaga tcaccggcca cgccgtctcc cccatctccg
     4501 ccgatcccgg cgacccggcc ctgcggcgcg cgcagcggcc gcggctcgtc ccctggaact
     4561 gaacccccca caggagcctg actgacccgt ggaactcgag tactccgacc gcaacagccg
     4621 ccgatcgaag atctacatcg ccgtcggcgt catcgtcgcg ctcctggtgg cggcgacggt
     4681 cttcctggcc ctccaggcca gcggcctgac tcaggaccgg gcggtcgaga tgcgcgacgt
     4741 cgtcgtcgcg acgcgcgaca tcccggctcg caaggccatc gaagaggggg atgtccaggt
     4801 gcgctccgtg ccggtggacc ccaccaacga caccgcgttc gcgtcgctgg acgaggtgct
     4861 cggccgcgtg gtcggcatcc cggtcgcgac ggggcagctg gtgacccgca acgtgctcgc
     4921 ctcgacgacg gagggccaga ccttctcgat cctcgagccg ggcgtcgagt tcgaccccga
     4981 ggggccccac ctgcgcgcgg tgagcgtcac cgtggccccg gcgaacgcgg tcgccggcac
     5041 gctggtggcc gggcagcgcg tggacctcat cgcgaccatg gccatcaacc cggagctggg
     5101 gcagacgccc gaggaggccg aggcccagtc ggcgcagttc ctgcccggtc cgtcgacgaa
     5161 ggtgaccctg cagtccatga cgatcctggc gcgcaacggg gacatctaca tcctccgctc
     5221 ggacgtcgag acggcggaga agatcgccga gctgaccgcg gccggcgcca cgttcaccat
     5281 ggtgctgcgg cctgaccagg acgaccggac ggccgagacc gatggctcga ccatcgacat
     5341 gctgatcgac gagttcgact tcccggtgcc cgtgcccccg cagttcgagg agcgacgcgc
     5401 cgggaactag cgagcgcgca gccgctcgag ggcggactcg acctcgcgtc gggtctcctc
     5461 gagcggcgcc gagccgtcga tcacgacgtc ggcgcgggcg accttctcct cctgggatga
     5521 ctggctggcc aggcggcggc gggctgcggc ctcgtccatg ccgcgcgacg cggccaggcg
     5581 ggcgatcgca tcggcctcgt cgcagcgcac cacccagagc tcgtcgcacg catcggccag
     5641 gggtgattcg aacagcttga tcacctcgac cgcggcgacc tcgacgttgc cctcggcgag
     5701 ctcggccagg cgcgcccgga tggcgccgcg gatgcgcggg tggaggatcg cctccaggtc
     5761 ggccagggcg ttcgggtcgg cgaagacgat gcggcccagc tccgcggcct cgtacgtccc
     5821 gaagcgcgcc tcgatcgcgc ggcgcgcatc ggcgtcgtta tggcggatct cgcgcgcgac
     5881 cacgtccgcg tcgatcgttg ccacgccgtg ctcgcgcagc atgcc
//
LOCUS       DASTYP010000030         5815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_110930, whole genome shotgun sequence.
ACCESSION   DASTYP010000030 DASTYP010000000
VERSION     DASTYP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110930"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..594)
                     /locus_tag="VFV59_01390"
     CDS             complement(4..594)
                     /locus_tag="VFV59_01390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5324514.1"
                     /translation="MTHLVRGERVFLRPFEARDNEAYRRWRADARVMATAGFGERAPL
                     SLAQVEKRLEQVTAEQGKDQYLFAICLIDDEHPIGEGSLFGIDRRVGSAELGIFIGEP
                     EEWGEGYGTDAVNALVDFGFGELRLERIWLNVWTENVPARRAYEKAGFVREGRVRHDL
                     YEHGTYTDGDIMSILRDEWLRRVASHEASEFTSPDR"
     gene            complement(617..1780)
                     /locus_tag="VFV59_01395"
     CDS             complement(617..1780)
                     /locus_tag="VFV59_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEU5324515.1"
                     /translation="MIEQPAVRDVVETRLRRAYGFEEVALVPGAVTADPAEVDLSVEI
                     GGHRLEIPFIAAAMDAVADADFAARMSRHGGMAVVNLEGLYARYEDATPIIDRVVAAE
                     TAQQAAAVLAEAYEQPVRDDLIRALITRIKAQGGLAAVATTPAAAWHHAELAADAGAD
                     LLLVQSQVSSARHLSTSGRVLSLADLTGGLRMPVLVGNTVSGEAAYQLMHQGAAGIFV
                     GVGPGAACTTREVLGIGVPQVSATLEVAAARDRFERETGRYVPVITDGGMKTGGDVAK
                     AIASGADAVMLGSPFAGALEAPGRGFNWGMAAPSPTLPRGTRIRIGTRLPLEQILFGP
                     AETTHGTQNLVGALRQSMAALGARTIREMHEVEMVIAPSIVTEGKSWQLSGRE"
     gene            1978..2382
                     /locus_tag="VFV59_01400"
     CDS             1978..2382
                     /locus_tag="VFV59_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324516.1"
                     /translation="MRASGWFSLSRGVYALVGAGALLLSLGGLSDGEALSDPVFPAGL
                     ALGILSLGAAAWADAPERWRAAVTWLGVVAVVVGLATFAWFVFGDPDISADVYPLFLV
                     PAAVLLVATAGVARGRLAAGALGAEPQRSTAR"
     gene            complement(2361..3986)
                     /locus_tag="VFV59_01405"
     CDS             complement(2361..3986)
                     /locus_tag="VFV59_01405"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEU5324517.1"
                     /translation="MPCVVPAEVISSSRRAIVWFRRDLRLDDNRTLESATAHAERVWP
                     VFVADPELLTAHASAPGRVAWFDANIRALDQRLRDAGSGLTVLRGRPEVVLRDFAAQV
                     GAEAVHAGTDEEPVAVARDARVAQVVDLRLVDDQRIVPPGELHAASGEPYRVYSPFRR
                     ALDARIQEAPDDLLRGADARLDRLAPPPAGEARLDGAPAPYELPPAGEAAAAERLRSF
                     LSHDIDAYGNDRDRPALDRTSRLSPYLRVGAISIRACWRAIAADRYSGHHGPQRRSAS
                     RMTEGSDTAEREAHAAAQLTFGLDRPSGHPARAVGLTGELTDPSVTARSATAEGAGRG
                     GETNGGARAWRGELAWREFFAHLLAAHPDVVEGSLRPEFQDLPWVDGAERDELLAAWR
                     IGHTGYPFVDAGMRQLMATGWMHNRARLVTASFLVKDLGIDWRIGESVFMDHLLDGDT
                     AQNNGNWQWVAGVGTDAAPYFRVLNPTLQAKRFDPDGAYVRRWVPEVAHLPAPEIHEP
                     WRSARPPDDYPTPIVDHAEARQRTLARYRAVER"
     gene            complement(4177..5358)
                     /locus_tag="VFV59_01410"
     CDS             complement(4177..5358)
                     /locus_tag="VFV59_01410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5324518.1"
                     /translation="MPTPFSQTVIAVVWDFDKTLIPGNMQSPLFAKYGVDERTFWAEV
                     DALHDFYLAHGARRVADDTLYLNHILEYVRAGAFAGLSNATLQELGADLNFHPGMPEF
                     LDQLRRSVEEDPTFERADITLEHYVVSTGLRPMIEGSAVRPFLTDVWACEFVEAHHPP
                     GYARPTQSQLFPSREITALGYQIDNTSKTRALFEINKGTNKHPEIHVNSHLPAEMRRV
                     PFEHMIYVADGPSDVPAWSVVRANGGLAFGVYQRGSQAQFEQINALLRDGRIHAFAEA
                     DYTPDSTAYLWLTTEVRRIADRIVEDRATALRRAVRAAPRHVDDTPSHTPTDAPMEQP
                     DEGPRQTDAPAEPASPAATSLADAPRPPLVEELRERLRHARIDDEAESSEPSSSSVRS
                     R"
     gene            complement(5445..>5815)
                     /locus_tag="VFV59_01415"
     CDS             complement(5445..>5815)
                     /locus_tag="VFV59_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324519.1"
                     /translation="TGMPIMVGGNGPNVTWRLAARYADELNLDGMTPDEVADALPTIR
                     ARCEEIDRDPDSLSVSVHAWWGTPEWREPGAARQEHLARYAELGLSRVIGLLRSTADS
                     DDALESLAEDARAAGMELAG"
BASE COUNT          763 a         2022 c         2158 g          872 t
ORIGIN      
        1 agatcagcgg tcaggtgacg tgaattcgga cgcttcgtgg gaggcgacgc ggcggagcca
       61 ctcgtcgcgc aggatggaca tgatgtcccc atcggtgtag gtgccgtgct cgtacaggtc
      121 gtggcggacc ctgccctcgc ggacgaagcc cgccttctcg tacgcgcgac gtgccggcac
      181 gttctcggtc cagacgttca gccagatccg ctccagccgc agctcgccga agccgaagtc
      241 gaccagcgcg ttgaccgcgt cggtgccgta gccctcgccc cactcctccg gctccccgat
      301 gaagatgccg agctccgcgc tgccgacgcg gcggtcgatg ccgaacagcg acccctcacc
      361 gatcgggtgc tcgtcgtcga tcaggcagat cgcgaacagg tactgatcct tgccctgctc
      421 ggcggtgacc tgctcgaggc gcttctcgac ctgcgccagg ctcagcggcg cgcgctcgcc
      481 gaagccggcc gtggccatga cgcgggcgtc cgcccgccag cggcgatacg cctcattgtc
      541 gcgcgcctcg aagggacgca ggaagacgcg ctcgcctcgg accaggtgcg tcacgtgcct
      601 gcggcccgga gagcgactac tcgcggccgg agagctgcca ggacttgccc tcggtcacga
      661 tcgacggtgc gatcaccatc tcgacctcgt gcatctcgcg gatcgtccgc gcacccaggg
      721 ccgccatcga ctggcgcagc gcgccgacca ggttctgggt gccatgggtg gtctcggccg
      781 gcccgaagag gatctgctcg agcggcaggc gggtgccgat cctgatgcgc gtcccgcgtg
      841 gcagcgtcgg cgagggggcg gccatgcccc agttgaagcc ccgtccgggt gcctcgagcg
      901 cgccggcgaa cggcgagccg agcatcaccg catcggcgcc ggaggcgatg gccttggcga
      961 cgtcgccgcc ggtcttcatg ccgccatcgg tgatgaccgg gacgtagcgg ccggtctcgc
     1021 gctcgaaccg atcgcgggcg gccgcgacct cgagggtcgc gctcacctgc ggcacgccga
     1081 tgccgagcac ctcgcgcgtc gtgcaggcgg caccgggtcc gacgccgacg aagatgccgg
     1141 cggcgccctg gtgcatcagc tggtaggcgg cctcgccgct caccgtgttg ccgaccagga
     1201 ccggcatgcg caggccaccg gtcaggtcgg ccagggagag gacgcggccg gacgtgctga
     1261 ggtgccgagc tgacgacacc tgcgattgga ccagcaacag gtccgctccc gcatcggcgg
     1321 cgagctcggc gtggtgccag gcggcggcag gcgtcgtggc gaccgcggcc aacccgccct
     1381 gcgccttgat gcgcgtgatc agcgcccgga tcagatcgtc gcggaccggc tgctcgtacg
     1441 cctcggccag gaccgctgcg gcctgttgcg ccgtctcggc ggccacaacc cgatcgatga
     1501 tcggcgtcgc gtcctcgtac cgggcgtaga ggccctcgag gttcacgacc gccatcccgc
     1561 cgtgccggct catgcgcgcg gcgaagtccg catcggccac ggcgtccatg gcggcggcga
     1621 tgaacgggat ctccaggcga tgcccgccga tctcgacgga aagatcgacc tccgccgggt
     1681 cggccgtcac cgcacccggg accagggcga cctcctcgaa cccgtaggcg cggcggagcc
     1741 gggtctcgac gacgtcacgg acggctggct gctcgatcaa cgtggacctc cggcggtacg
     1801 aaaaacccgc gcggcatggc ggcgcgggct gggctcgatg cgggcgacgg tgacggcgcg
     1861 aatcgggtgc acgtgggtgg cggtgcggct ttcgcgaagt atagggcgtc ggggtcttcc
     1921 cagacgaggc cttcacggcg aggatgggcg cctcgtcatc cacggacgga ggaaacgatg
     1981 cgcgcatccg gctggttctc gctgtctcgc ggtgtctatg ccctcgtcgg cgccggggcg
     2041 ctgctgctgt cgctcggcgg gctgagtgac ggtgaggccc tgagcgaccc cgtcttcccg
     2101 gccggcctcg ccctcggcat cctctcgctg ggggcggcgg cctgggccga tgctccggag
     2161 cggtggcgcg cggccgtgac atggctgggc gttgtcgcgg tggtcgtcgg gctggcgacc
     2221 ttcgcctggt tcgtgttcgg cgatcccgac atcagcgccg atgtgtaccc gttgttcctc
     2281 gtccccgcgg ccgtgctgct cgtcgcgacg gccggcgtgg cccgtgggcg cctggcggcg
     2341 ggcgccctcg gggccgaacc tcagcgctcg acagcgcggt agcgcgcgag ggtccgctgc
     2401 cgcgcctcgg cgtggtcgac gatcggcgtg ggatagtcgt cgggtggtcg cgcggagcgc
     2461 cacggttcgt ggatctcggg ggcgggaagg tgcgccactt cggggaccca tcggcgcacg
     2521 tacgctccat cggggtcgaa gcgcttcgcc tgcagagtcg ggttcaggac gcggaagtac
     2581 ggcgcggcat cggtgcccac gccggccacc cactgccagt tcccgttgtt ctgcgccgtg
     2641 tcgccgtcca gcaggtggtc catgaagacc gattcaccga tgcgccagtc gattcccagg
     2701 tccttgacca ggaagctcgc cgtgaccagg cgagcccggt tgtgcatcca gccggtcgcc
     2761 atcagctggc gcattccggc gtccacgaag gggtaccccg tgtggccgat gcgccacgcg
     2821 gccagcagct cgtcgcgctc cgcgccgtcg acccagggaa ggtcctggaa ctcgggacgc
     2881 aggctgccct cgacgacgtc cggatgggcc gccaggaggt gcgcgaagaa ctcgcgccag
     2941 gccagctcgc cgcgccatgc cctcgcccca ccgttcgtct cgcccccgcg cccggcccct
     3001 tcggcggtgg cgctgcgggc ggtgaccgat ggatcggtca gttcgcccgt gaggcccact
     3061 gcccgtgccg ggtgtccgct cggtcggtca aggccgaacg tgagctgcgc cgcggcgtgc
     3121 gcctcacgct ccgcggtgtc cgatccctcg gtcattcggc tcgcggagcg gcgctgcggt
     3181 ccgtggtgac cgctgtatcg gtcggcggcg atcgcgcgcc agcaggcgcg gatgctgatg
     3241 gcaccaaccc gcaggtaggg cgagagccgg ctcgtccggt ccagcgccgg ccggtcgcgg
     3301 tcgttgccgt aggcgtcgat gtcatggctc aggaacgacc gcaggcgctc ggccgccgcc
     3361 gcctccccgg ccggcggcag ctcgtacggt gccggcgcac cgtccagacg cgcctccccg
     3421 gcggggggcg gagcgaggcg atcgagtcgt gcatcggcgc cgcgcaggag gtcgtcgggc
     3481 gcctcctgga tccgggcgtc gagcgcgcga cggaagggcg agtacacgcg gtacggctct
     3541 ccgctggccg catgcagctc accgggcgga acgatgcgct ggtcgtccac gagtcgcagg
     3601 tccacgacct gggcaacccg ggcgtcgcgc gcgaccgcga ccggctcctc gtccgtcccg
     3661 gcgtgcacgg cctccgcgcc gacctgcgcg gcgaagtccc gcagcacgac ctccgggcga
     3721 ccgcggagga cggtcaggcc ggagccggca tccctcaggc gctggtcgag cgcgcggatg
     3781 ttggcgtcga accaggcgac ccgtcccggt gcggaagcgt gagccgtcag cagctcgggg
     3841 tcggcgacga agaccggcca cacgcgctca gcgtgcgcgg tggcggactc gagcgtccgg
     3901 ttgtcatcaa ggcgcaggtc ccgtcgaaac cagacgatgg cgcggcgtga cgaggaaatc
     3961 acttccgctg gtacgacgca gggcatgtgc cggatcttgg tccgcagtct cggcctgtcg
     4021 cgacaccgcg gcgtggcgcg ccccttccgt cacgttcccg ccatccatcg cgacgtgcga
     4081 tgcgcgtgtg cggccaacgg gggccgctca tgcgtcgggg ttgcgatgag tcgcgaggtc
     4141 agcgctggac cacggctccg gcggagtcgc cgcgatctat cgagagcgaa ccgacgagga
     4201 cgagggctcc gacgattccg cctcatcgtc gatccgggcg tggcgcagcc gctcccgcag
     4261 ctcctcaacg agcggcggcc gtggcgcatc ggccagcgag gtcgccgcgg gcgacgccgg
     4321 ctccgccggc gcatcggtct ggcgcggccc ttcgtccggc tgctccatcg gcgcatccgt
     4381 gggcgtgtgg gatggagtgt cgtcgacgtg ccgcggtgcc gctcgcaccg cgcgccgcag
     4441 cgcggtggcc cggtcctcga cgatccggtc cgcgatccgg cggacctcgg tggtgagcca
     4501 caggtaggcg gtcgagtccg gggtgtagtc ggcttcggcg aaggcgtgga tgcggccgtc
     4561 gcgcagcagg gcattgatct gctcgaactg cgcctgcgac ccgcgctggt agacgccgaa
     4621 ggcgaggccg ccgttggccc ggacgaccga ccaggcgggg acgtcggaag ggccgtcggc
     4681 gacgtagatc atgtgctcga acggcacccg gcgcatctcc gccggcaggt gcgagttgac
     4741 gtgaatctcc gggtgcttgt tggtgccctt gttgatctcg aacagcgcgc gcgtcttgct
     4801 ggtgttgtcg atctggtagc ccagcgcggt gatctcgcgg ctcgggaaca gctgggactg
     4861 cgtcgggcgg gcgtagccgg gcgggtggtg cgcctccacg aactcgcagg cccacacatc
     4921 ggtgaggaag gggcggacgg cgctgccctc gatcatcggg cgcaggccgg tgctcacgac
     4981 gtagtgctcg agcgtgatgt cggcgcgctc gaaggtcggg tcctcctcga cggaccggcg
     5041 gagctggtcg aggaactccg gcatgccggg gtggaagttc aggtcggcgc ccagctcctg
     5101 gagcgtcgcg ttcgacaggc cggcgaaggc gccggcgcgc acgtactcga ggatgtggtt
     5161 caggtagagc gtgtcgtcgg cgacgcggcg cgcgccgtgg gcgaggtaga agtcgtggag
     5221 cgcatcgacc tcggcccaga acgtccgctc gtcgacgccg tacttcgcga agagcggcga
     5281 ctgcatgttg cccgggatca gggtcttgtc gaagtcccag accaccgcga tgacggtctg
     5341 gctgaacggt gtcggcatca cgctcctgtg cgcgcggtca acggacggcc agtctagtca
     5401 cgtcggtcaa ccgctccgaa tgggccgatg gtcacgttcg cgcgtcagcc agcgagctcc
     5461 atgccggcgg cccgcgcatc ctccgccagc gactcgaggg cgtcgtcgga gtcggcggtc
     5521 gaccggagca gaccgatgac ccggctcagg cccagctccg cgtaccgggc caggtgctcc
     5581 tggcgagcgg cgccgggctc gcgccattcc ggcgtgcccc accaggcgtg gacgctgacg
     5641 gacagcgagt cggggtctcg gtcgatctcc tcgcagcgcg cgcggatggt cggcagcgcg
     5701 tcggccacct cgtcgggcgt catgccgtcc aggttcagct cgtcggcgta gcgggcggcg
     5761 aggcgccagg tcacgttcgg accgttgccg ccgaccatga tcggcatgcc ggtgg
//
LOCUS       DASTYP010000031        10579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_293400, whole genome shotgun sequence.
ACCESSION   DASTYP010000031 DASTYP010000000
VERSION     DASTYP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293400"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="rlmB"
                     /locus_tag="VFV59_01420"
     CDS             <1..635
                     /gene="rlmB"
                     /locus_tag="VFV59_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU5324520.1"
                     /translation="RHITDAARRMGLPTDRVPADALTRLAGFDGHQGVLLEVGERRFA
                     DLPEILAHARSRAQDPFVLVLEHLQDPTNFGSLLRAAEAAGVHGVVYPERGAAPLTAA
                     AVKASAGASEHLRLARLPTIGEAIHELKLAGLRLVAADQDAPSTAWDADLAGPVAVVV
                     GSEGSGLSGATRRRCDVLVRFPMAGQVGSLNAATAGSLLLFEVVRQRSRA"
     gene            872..946
                     /locus_tag="VFV59_01425"
     tRNA            872..946
                     /locus_tag="VFV59_01425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:904..906,aa:Thr,seq:tgt)
     gene            1042..1127
                     /locus_tag="VFV59_01430"
     tRNA            1042..1127
                     /locus_tag="VFV59_01430"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1076..1078,aa:Tyr,seq:gta)
     gene            1271..1346
                     /locus_tag="VFV59_01435"
     tRNA            1271..1346
                     /locus_tag="VFV59_01435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1304..1306,aa:Thr,seq:ggt)
     gene            1337..2467
                     /locus_tag="VFV59_01440"
     CDS             1337..2467
                     /locus_tag="VFV59_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324521.1"
                     /translation="MGSSLGPPQTLDTLAGGSFARMDPNVVLPFLSSLLSFVFAVMVA
                     DQWVRRRRPYQLVWTVGLLWYGISAGTEFIGGAFGWSEPLYRAWYLIGAFGVAAYLGL
                     GTVFLLEKTRFGYFVAFSIFAGGLFSILSALARAKEGDPVSAGVVPMVVGLSAVAAIV
                     IALLTHYRRSLVAPTTAAIVVAASVAAAILTFTAPVAAPGYHIDVTGVPTGEAFPADL
                     RVLTPPFNIAGAFALVFGAAYSAYIFMPKRRVLRIKGGPPVIGALGRGIAVVVNFFAS
                     LPGTVRALANGSLNSRVPATILIAIGGFIPGWTSGLNRFGITWAFFLGELLGVVFIFL
                     GFLVSIEVFSDVRLPFTRVVLRRRDRGRELAPAEAEAATAAR"
     gene            2574..2738
                     /gene="rpmG"
                     /locus_tag="VFV59_01445"
     CDS             2574..2738
                     /gene="rpmG"
                     /locus_tag="VFV59_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015022386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5324522.1"
                     /translation="MAKAENRPVIALACTVCKERTYSTRKNKKNDPGRLELNKFCPRC
                     RQHQLHRETK"
     gene            2756..2831
                     /locus_tag="VFV59_01450"
     tRNA            2756..2831
                     /locus_tag="VFV59_01450"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2789..2791,aa:Trp,seq:cca)
     gene            2892..3074
                     /gene="secE"
                     /locus_tag="VFV59_01455"
     CDS             2892..3074
                     /gene="secE"
                     /locus_tag="VFV59_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU5324523.1"
                     /translation="MLTGIRRYLVESWAELKKVAWPTRQTVIRLTLLVIGVSAAVGVY
                     IFVLDSIFNTLVEQVL"
     gene            3074..3694
                     /gene="nusG"
                     /locus_tag="VFV59_01460"
     CDS             3074..3694
                     /gene="nusG"
                     /locus_tag="VFV59_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU5324524.1"
                     /translation="MADETTTEAVNEAREAARLSREAPHAGDSADGAKKQWYVIHTYS
                     GYENKVKANLEHRIESMGVEDQIFQVLVPMEEEIEIRNGQRQTVNRKVFPGYVLVEMA
                     MSDESWYVVRNTPGVTSFVGSGNRPLPLDEDEVKKILKQMGVETPKFKVQFAKGQSVR
                     VKDGPFAEFIGTVDEVNPERNKVKVLVSIFGRETPVELDFLQIEKL"
     gene            3782..4207
                     /gene="rplK"
                     /locus_tag="VFV59_01465"
     CDS             3782..4207
                     /gene="rplK"
                     /locus_tag="VFV59_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU5324525.1"
                     /translation="MAKKVRAMLKVQAPAGAATAAPPVGPALGQHGINIMEFIKAFNE
                     RTADLRGYVVPAEITVFEDRSFTFVTKAPLAAALLKRAAGVESGSAAPNKEKVGRVTR
                     DQVREIAELKMADLNANTVEQAMKIIEGTARSMGIEVAS"
     gene            4207..4914
                     /gene="rplA"
                     /locus_tag="VFV59_01470"
     CDS             4207..4914
                     /gene="rplA"
                     /locus_tag="VFV59_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU5324526.1"
                     /translation="MAKHGKKYLAAAKLVDAERRYEIGEAAELLPQLSTSAFDGTVEA
                     HLRLGVDPRHADQLVRGTVVLPHGTGRTTRVIVFAQGEKAQEALRAGADEVGADDLVK
                     KIDDGWFEFDVAVATPDMMGTVGRLGKKLGPRGLMPNPKSGTVTFDIERAVGEVKSGR
                     VEFKVDRAGIVHVPVGRASFTPDQLVANVATLVDAVNRAKPSGAKGTYMRTLTLAPTM
                     GPGVRIDIPSALAAAAG"
     gene            5151..5684
                     /gene="rplJ"
                     /locus_tag="VFV59_01475"
     CDS             5151..5684
                     /gene="rplJ"
                     /locus_tag="VFV59_01475"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU5324527.1"
                     /translation="MPTDEKRQAVAELAELLRSSKAMAVADYRGLRVSDMQSVRRTLR
                     DSGVQLHVAKNRLLKIAADEAGRSELKPMLTGPTALATIDGDEAAMAKALADALRPYS
                     RVVSVRGGMLGDRAIDAAQLTQLATLPSREVLLGKIAGGMAAPLTGMAGVLAANLRNL
                     VGVLSAVADKKQQSEAA"
     gene            5742..6134
                     /gene="rplL"
                     /locus_tag="VFV59_01480"
     CDS             5742..6134
                     /gene="rplL"
                     /locus_tag="VFV59_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU5324528.1"
                     /translation="MDKDQILEAIDSMTVLELSELVKAFEERYGVTAAAPVAVAAGAP
                     AAAGDGAAAGGAAAEEQTEFDAVLTDVGANKILVIKAVRELTGLGLKEAKDLVDAAPK
                     PIKEGVAKEEAEQVKEKLAEAGATVEVK"
     gene            6275..7267
                     /locus_tag="VFV59_01485"
     CDS             6275..7267
                     /locus_tag="VFV59_01485"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU5324529.1"
                     /translation="MARTEAPELPDELSWDEHELLARIAHRWYLDGRTQGAIAEEFGL
                     SRPKVQRLLERARATGVVEIHVEPPQGVALDLEGLLLETFPLTEAIVSSLRPDADSVR
                     AGVARSAARFLERRLHDGMVVAVSHGRDIGEVPRFFRPGGPLDCTFASAMGGSPRIDA
                     PTNPNEICRALAERSGGRAESLFAPAYVESLEIRDRLLEQEAVAHALQVAARADMAMV
                     GIGGTDDDCTMVRSGSLSLEEIAELRSQGAVGDVLGNYVDIDGRLIPAPHDSRLIGLS
                     IDDLRRIGMVVAVASGDEKPRAVLGALRAGIVDVLIVDEANARAVIGLARAGGD"
     gene            7269..8117
                     /locus_tag="VFV59_01490"
     CDS             7269..8117
                     /locus_tag="VFV59_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU5324530.1"
                     /translation="MPVVPTQQIVNQAFERRYGVAAINVVNDLTMEAVLAAAEELRSP
                     LIVQTSVKTVKSIGADVLMAMWRAMTDRISVPVALHLDHCPEREVITECLAKGWNSVL
                     FDGSKLSVEENKRQCIEVVAEARPYGALVEGEIEGFKRVEEMADDEAVEEQTLEVAVD
                     FIRSTGVDVFAPAIGNAHGMYRATPKLDSQRVTDIVEATGIPVALHGGTGMTPEQFSD
                     LIARGCAKVNISTALKIRFMQSGYDYMTEHPGKYDPPSLFDVQRAAVKEMAAEHIRMF
                     GSEGQA"
     gene            8117..8890
                     /locus_tag="VFV59_01495"
     CDS             8117..8890
                     /locus_tag="VFV59_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5324531.1"
                     /translation="MPALIFDCDGVLADTERDGHRVAFNQTFREFGLPIEWSEEVYAE
                     KLRTAGGKERMATDLTPEFLRANGLPDDDEARTDLLARLHKRKTAIYTELVAAGRLPT
                     RPGISRIIAEAQDAGWEVAVASTSAEPSVRAILEQAVGPERVGRFDVVLAGDVVEKKK
                     PAPDIYLLALERLGLPAADVLVIEDSRNGILAAAGAGLRCVMTVNGYTEDEANDEAIL
                     VVSSLGDPGSEPIRVIANRSAATPGEFVTLADLQRCLAS"
     gene            8908..9579
                     /gene="dhaL"
                     /locus_tag="VFV59_01500"
     CDS             8908..9579
                     /gene="dhaL"
                     /locus_tag="VFV59_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEU5324532.1"
                     /translation="MAQPALDDVDLVLKTMARTIVDHADEFAHLDSIVGDGDFGFSLR
                     NGFEVVDAEYDTFDHSSVGAILKKIGLVISGKVGGVSGPIWGTAFLRAGASAGDKTEL
                     STDDVITMLRAAVEGIKQRGQSDVGDKTLLDALVPAIDSLEESFREPIEDHGVAAIQR
                     AADVAVRAAENTKGMLAMRGRAAYTGERSRDSVDAGATAVGVILQDISAAWRAKYSNG
                     QEARA"
     gene            9576..10571
                     /locus_tag="VFV59_01505"
     CDS             9576..10571
                     /locus_tag="VFV59_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU5324533.1"
                     /translation="MKKFVNDPKQFVPEFLEGIALASNGALKYEPKYNLIYRADMPKP
                     DRVSIIQGSGSGHEPAHTMIVGPGMLDAACPGDVFAGPPVEFVTETSKLMDTPAGVLY
                     IVNNYTGDRTAWEMAKEIQEAEGQKIGYLLVNDDVAVKDSTWTVGRRGVAGNFFVIKA
                     AAAMADKGGSLEECVRVGEKVIGNVRTMGIALSSCTPPVRGTPLFDIGDDEMEIGIGI
                     HGEPGRERAKMRPANEIVDILLDAIVPDLPFESGDEVALMVNGLGGTPISELYLLYGI
                     AHKKLEEKGINVFRSYVGEYCTSLEMAGAHLSLLKVDDEIKELLLAPAEVAYRVF"
BASE COUNT         1707 a         3660 c         3679 g         1533 t
ORIGIN      
        1 tgcgccacat caccgatgcg gcgcgccgca tgggcctgcc caccgaccgc gtgccggctg
       61 acgccctgac caggctggcc ggcttcgacg gccaccaggg cgtgctgctc gaggtggggg
      121 agcgtcgctt cgccgacctc ccggagatcc tggcgcacgc ccggtcgcgc gcccaggacc
      181 cgttcgtgct cgtcctggag cacctccagg accccaccaa cttcgggtcg ctgctgcgcg
      241 ccgcggaggc cgccggcgtc catggcgtcg tctacccaga acgcggtgcc gcgccgctca
      301 ccgccgcggc cgtgaaggcc agcgccgggg ccagcgagca cctgcgcctg gcccgactgc
      361 cgaccatcgg cgaggccatc cacgagctga aactggccgg cctgcggctg gtcgccgccg
      421 accaggacgc gccgtcgacc gcgtgggacg ccgacctggc cgggccggtc gcggtggtcg
      481 tgggctccga ggggagcgga ctgtccggcg ccacgcgacg ccggtgcgac gtgctggtcc
      541 gcttcccgat ggccggccag gtggggagcc tcaacgcggc gaccgcgggg agcctgctgc
      601 tgttcgaggt cgtccgccaa cggtctcgcg cctgagcaat cggtccggca tgcgtcatgc
      661 atccgcgcgg ggcgtgagca acgaccgccg acccatccgc gaacctcgga tccgacgctc
      721 ggagctgttg agtgcggcgc cacccggcgc gcgcgtgctg gcggcgctct tcccggcgcc
      781 gcggcacacc gtccccgtct ggagccgccc gcgaccccag ccgacccggc cgccgacgcg
      841 cccctgagtt gctacactcg gccgcacgcc ggccgaggta gctcaatggc agagcatcgg
      901 ttttgtaaac cgacggttgt gggttcgact cccaccctcg gctccagcac aggccagccg
      961 acgggtgccg cttgaagacg acgcggcttc ttggatatac tcgcaccggc cggcgatgca
     1021 ccacgcgtcg gtcgttttcg tgggtaggtt gagcaggtgg tgagctccgc tgactgtaga
     1081 tcagccgtct ttcgactgtg ggggttcgat tccctccctg cccaccatcc ttcccatccg
     1141 aagacctccg cccggtgaat ctcagctcga gaccgaggtt taccgagttt gtcgaacagt
     1201 tgtcgaggat cgatcgatcc gcgacactgc acgcgtccgc cgagaggcgg atggcggtgc
     1261 catcggccgc gcccacgtag ctcagtaggc agagcacttc cttggtaagg aagaggtctc
     1321 cggttcgagc ccggacgtgg gctccagcct cggtccgccg cagaccctcg acaccctggc
     1381 tggaggcagc ttcgctcgca tggatcccaa cgtcgtcctc ccgttcctga gctccctgct
     1441 ctcgttcgtg ttcgccgtca tggtcgcgga ccagtgggtc cggaggcggc ggccgtacca
     1501 gctggtgtgg acggtcggcc tgctctggta cggcatcagc gcggggaccg agttcatcgg
     1561 cggggccttc ggctggagcg agccgctgta ccgggcctgg tacctcatcg gcgcattcgg
     1621 cgtggcggcg tacctgggtc tgggcaccgt cttcctgctc gagaagaccc gcttcggcta
     1681 cttcgtcgcc ttcagcatct tcgccggcgg cctcttcagc atcctctcgg cgctcgcccg
     1741 ggccaaggag ggggatccgg tctccgccgg cgtcgtcccg atggtcgtgg gcctctcggc
     1801 cgtcgcggcc atcgtgatcg ccctgctcac gcactaccgt cggtcactcg tcgcgccgac
     1861 cacggccgcc atcgtcgtgg cggcatcggt cgcggccgcg atcctcacgt tcacggcgcc
     1921 ggtggccgcg cccggctatc acattgacgt caccggcgtg cccaccggcg aggcgttccc
     1981 ggccgatctg cgcgtcctga cgccgccgtt caacatcgcc ggcgccttcg ccctcgtctt
     2041 cggggccgcc tactcggcgt acatcttcat gcccaagcgt cgggtgctgc gcatcaaggg
     2101 cggcccgccg gtcatcggcg ccctcggccg cgggatcgcc gtggtcgtca acttcttcgc
     2161 ctcgctgccc gggaccgtcc gcgcgctggc aaacggctcg ctcaactcgc gcgttccggc
     2221 caccatcctg attgccatcg gcggcttcat cccgggctgg accagcggcc tgaaccgatt
     2281 cggcatcacc tgggccttct tcctgggcga gctgctgggc gtcgtcttca tcttcctcgg
     2341 cttcctggtg agcatcgagg tcttcagcga cgtgcggctg cccttcaccc gcgtcgtgct
     2401 gcgccggcgt gaccggggac gcgagctcgc gcccgcggaa gcggaagccg cgactgcggc
     2461 gcgttgacac cgacgccggt cgcaccgtag aatctcgggg ctttcccccg gtctccaggc
     2521 cctagttcga cgtggccggg ggcgggtgga caacacatcg aaacgagtga tccgtggcca
     2581 aggctgagaa ccgtcccgtg atcgcgctgg cgtgcaccgt ctgcaaggag cgcacgtaca
     2641 gcacacggaa gaacaagaag aacgaccccg gtcgcctgga gctgaacaag ttctgcccgc
     2701 gctgccgcca gcaccagctg caccgagaga ccaagtagga ggcggcgttc gcgatagggg
     2761 tgtagctcaa ttggtagagc actggtctcc aaaaccagcg gttgcaggtt cgagtcctgt
     2821 cgcccctgcc accacccgcg aacgcatccg ccggacccga acccacccgt agcaggaagt
     2881 acgtaccgaa cgtgttgacc ggcatccgcc gatacctcgt cgagtcctgg gccgagctca
     2941 agaaggtcgc ctggccgacg cgccagaccg tgatccgcct gacgctgctc gtcatcggcg
     3001 tctcggccgc ggtcggggtc tacatcttcg tgctcgactc gatcttcaac acgctcgtgg
     3061 agcaggtgct ctgatggccg atgagacgac gaccgaggcg gtgaacgagg cccgtgaggc
     3121 cgcacgcctc tcgcgcgagg cgccgcacgc cggtgacagc gccgatggcg ccaagaagca
     3181 gtggtacgtg atccacacgt actccggcta cgagaacaag gtcaaggcga acctcgagca
     3241 ccgcatcgag tcgatgggcg tggaggatca gatcttccag gtcctggtgc cgatggagga
     3301 ggagatcgag atccgcaacg gccagcgcca gacggtgaac cgcaaggtct tccccggcta
     3361 cgtgctggtc gagatggcga tgagcgacga gtcgtggtac gtcgtgcgca acacgcccgg
     3421 cgtgacctcc ttcgtcggct cgggcaaccg accgctgccg ctcgacgagg acgaggtcaa
     3481 gaagatcctc aagcagatgg gcgtcgagac gccgaaattc aaggtccagt tcgccaaggg
     3541 ccagagcgtt cgcgtcaagg acggcccgtt cgccgagttc atcggcaccg tcgacgaggt
     3601 caacccggag cgcaacaagg tcaaggtgct ggtcagcatc ttcggccgcg agacgccggt
     3661 ggagctcgac ttcctgcaga tcgagaagct gtaggaccga ttccgacggc gggatgacgg
     3721 ccgccgcaac agcagagagg gaggggcgcg aaccgccccg tcagcacctc ggaggaacca
     3781 catggcaaag aaggtccgcg cgatgttgaa ggtccaggca ccggccggtg ccgcgaccgc
     3841 cgcgccgccg gtcgggcccg cgctcggcca gcacggcatc aacatcatgg agttcatcaa
     3901 ggccttcaac gagcgaaccg ccgacctgcg cggctacgtc gtcccggccg agatcacggt
     3961 cttcgaggac cgatccttca ccttcgtcac caaggcgccg cttgccgccg cgctgctgaa
     4021 gcgcgccgcc ggcgtcgagt ccggctcggc ggcgccgaac aaggagaagg tcggtcgcgt
     4081 gacgcgcgac caggtgcgcg agatcgccga gctgaagatg gccgatctga acgccaacac
     4141 cgtcgagcag gcgatgaaga tcatcgaggg taccgcgcgc tcgatgggca tcgaggtggc
     4201 ctcctgatgg cgaagcacgg caagaagtac ctggcggccg ccaagctggt ggacgccgag
     4261 cgccgctacg agatcggcga ggcggccgag ctgctgccgc agctgtcgac cagcgccttc
     4321 gacggcaccg tcgaggcgca cctgcgcctg ggggtcgatc cgcgccacgc cgaccagctc
     4381 gtgcgcggga ccgtggtcct gccgcacggc accgggcgca ccacgcgcgt catcgtcttc
     4441 gcccagggcg agaaggcgca ggaggccctg cgcgccgggg cggacgaggt cggcgccgac
     4501 gacctggtga agaagatcga cgatggctgg ttcgagttcg acgtcgccgt cgcgacgccc
     4561 gacatgatgg gcacggtcgg ccgcctcggg aagaagctgg gaccccgcgg cctcatgccc
     4621 aacccgaagt ccggcacggt gaccttcgac atcgagcgcg ccgtcggcga ggtgaagtcg
     4681 ggccgggtcg agttcaaggt cgaccgggcc ggtatcgtgc acgtcccggt gggccgcgcg
     4741 agcttcacgc ccgatcagct ggtggcgaac gtcgcgacgc tggtcgacgc ggtgaaccgc
     4801 gccaagccgt ccggcgccaa gggcacctac atgcggacgc tgaccctcgc gccgacgatg
     4861 ggccccggcg tccggatcga catcccgtcc gcgctggccg cggccgccgg ctgagaccgc
     4921 cggaggacat cgaggcgcgc atcgggtagg atgcacgccg atcaaccgaa gatccgccga
     4981 agaccgcgat gcgtcccggg ccaccagccc ggaccaattg cggcgcgcaa gcgccgcggc
     5041 acgcgcaggc acgaagaacg aactcgtcat tgaacggcgg ttctcgtgct cacgcgtgca
     5101 cggggccgcc gtttcggcgt tcttcacctg aggagaacga gaaggaaccg atgccaaccg
     5161 acgagaagcg ccaggccgtc gccgagctgg cggagctgct ccgctccagc aaggccatgg
     5221 ccgtggccga ctaccggggc ctcagggtct cggacatgca gtcggtgcgg aggaccctgc
     5281 gtgacagcgg cgtccagctc cacgtcgcca agaaccgcct gctgaagatc gccgccgacg
     5341 aggcgggccg ctccgagctc aagccgatgc tcaccggtcc gaccgcgctg gccacgatcg
     5401 acggtgacga ggcggccatg gcgaaggcgc tggccgatgc cctccgaccg tactcccgcg
     5461 tcgtcagcgt gcggggtggg atgctcggtg accgcgccat cgacgcggcc cagctgacgc
     5521 agctcgccac cctgccgtcc cgcgaggtcc tgctcggcaa gatcgccggc gggatggcgg
     5581 cgcccctcac cggcatggcg ggcgtgctcg ccgcgaacct gcgcaacctc gtgggtgtgc
     5641 tgagtgcggt ggccgacaag aagcagcaga gcgaagcggc ctgacggtcg ccgatcgaca
     5701 cgaacacgac aggagaaccg ataccgatgg cgaccaagac cctggacaag gaccagatcc
     5761 tcgaggcgat cgacagcatg accgtcctcg agctcagcga gctggtgaag gccttcgagg
     5821 agcgctacgg cgtgaccgcc gccgccccgg tggccgtcgc ggccggcgcc cccgcggccg
     5881 ccggtgatgg cgccgctgcc ggtggggcgg cggccgagga gcagaccgag ttcgacgccg
     5941 tcctcaccga tgtcggcgcg aacaagatcc tcgtcatcaa ggccgtccgc gagctgaccg
     6001 gcctgggcct caaggaggcc aaggatctcg tcgacgccgc gcccaagccg atcaaggagg
     6061 gcgtggccaa ggaggaggcc gagcaggtca aggagaagct ggccgaggcc ggcgcgaccg
     6121 tcgaggtcaa gtaaccgacc gtcttccctc cgttcgcgac acgagcgccg gcccttcggg
     6181 gtcggcgctc gtcgctgtcc ggggacgcga aggctcgacg gcggccgggc ggccctgcta
     6241 gactgagcat ctgcgcacat ctgagcaatt gctcatggcg cgcacggagg ctcccgagtt
     6301 gccggacgag ctcagctggg acgagcacga gctgctcgcg cgaatcgcgc accgctggta
     6361 cctggacggc cgcacccagg gcgcgatcgc cgaggagttc ggactctccc gtcccaaggt
     6421 ccagcgcctg ctcgaacggg cgcgggccac gggcgtcgtc gagatccacg tcgagccgcc
     6481 ccagggcgtc gcgctcgacc tggagggcct gctgctcgag acgttcccgc tgacagaggc
     6541 gatcgtcagc tcgctccggc ccgacgccga ctcggtacgg gccggcgtcg cacgaagtgc
     6601 cgcgcgcttc ctggagcggc gcctccacga cggcatggtc gtcgccgtca gccacggccg
     6661 ggacatcggc gaggtcccgc gcttcttccg gccgggcggc cccctggact gcacgttcgc
     6721 cagcgccatg ggcggctcgc cgcgcatcga cgcgccgacc aacccgaacg agatctgccg
     6781 cgcgctggcg gagcgcagcg ggggccgcgc cgagagcctg ttcgcgcccg cctacgtcga
     6841 gagcctcgag atccgcgacc ggctcctgga gcaggaggcg gtcgcgcacg ccctgcaggt
     6901 ggcggcgcgc gcggacatgg ccatggtcgg catcggcggg accgatgacg actgcacgat
     6961 ggtgcgctcc ggcagcctct cgctcgagga gatcgccgag ctccggtcgc agggcgcggt
     7021 cggcgacgtg ctcggcaact acgtcgacat cgatggccgg ctgatcccgg ccccccacga
     7081 cagccggctg atcgggctgt cgatcgacga cctgcgcagg atcggcatgg tggtcgccgt
     7141 cgcctcgggc gacgagaagc cccgcgccgt cctcggcgcg ctgcgcgccg ggatcgtcga
     7201 tgtcctgatc gttgatgaag cgaacgcgcg cgccgtcatc ggtctcgcgc gggcaggagg
     7261 tgattagggt gcccgtggtt cccacccaac agatcgtgaa ccaggccttc gagcgccgct
     7321 acggggtggc ggccatcaac gtcgtcaacg acctcaccat ggaggccgtg ctggccgccg
     7381 cggaggagct tcgctcgccg ctcatcgtcc agacgtcggt caagaccgtg aagtccatcg
     7441 gcgccgacgt gctgatggcc atgtggcgcg ccatgacgga tcggatcagc gtgccggtgg
     7501 cgctccacct cgaccattgc ccggagcgcg aggtcatcac cgagtgcctg gccaagggct
     7561 ggaactcggt gctcttcgac ggctcgaagc tgagcgtcga ggagaacaag cgccagtgca
     7621 tcgaggtcgt cgccgaggcc aggccgtacg gggcgctggt cgaaggcgag atcgagggct
     7681 tcaagcgcgt cgaggagatg gccgacgatg aggccgtcga ggagcagacg ctggaggtcg
     7741 cggtcgactt catccggtcg accggcgtcg acgtcttcgc ccccgcgatc ggcaacgcgc
     7801 acgggatgta ccgcgccacg ccgaagctgg acagccagcg cgtcaccgac atcgtcgagg
     7861 ccaccggcat tccggtggcg ctgcacggcg gcacggggat gacgccggag cagttcagcg
     7921 acctcatcgc gcgcggctgc gccaaggtca acatctcgac cgcgctgaag atccgcttca
     7981 tgcagtcggg ctacgactac atgaccgagc atcccggcaa gtacgaccca ccgtcgctgt
     8041 tcgatgtgca gcgtgcggcg gtcaaggaga tggcggccga gcacatccgc atgttcggca
     8101 gtgaggggca ggcgtgatgc ccgccctgat cttcgactgc gacggcgtcc tggccgatac
     8161 ggagcgtgac ggccaccgcg tcgcgttcaa ccagaccttc cgcgagttcg gcctcccgat
     8221 cgagtggtcc gaggaggtgt atgccgagaa gctgcggacc gcgggcggca aggagcgcat
     8281 ggcgaccgat ctcacgcccg agttcctccg cgccaatggc ctgcccgacg acgacgaggc
     8341 tcggacggat ctcctcgccc ggctccacaa gcgcaagacg gcgatctata ccgagctggt
     8401 ggcggccggg aggctgccga cgcggcccgg catcagccgc atcatcgccg aggcgcagga
     8461 cgcgggctgg gaggtcgccg tcgcgtcgac ctcggccgag ccatccgtcc gcgccatcct
     8521 cgagcaggcg gtcggcccgg agcgggtcgg gcgcttcgac gtcgtcctcg ccggggacgt
     8581 ggtggagaag aagaagcccg cgccggacat ctacctgctc gcgctcgagc ggctggggct
     8641 gccggccgcg gatgtcctcg tcatcgagga ctcgcgcaac gggatcctcg ccgccgctgg
     8701 cgcggggctc cgctgcgtga tgacggtcaa cggatacacg gaggacgaag ccaacgacga
     8761 agccatcctg gtcgtctcgt cgctcgggga tccgggcagc gagccgatcc gcgtgatcgc
     8821 caaccggagc gccgccacgc cgggggagtt cgtgaccctc gcggacctgc agcgctgcct
     8881 cgcgtcgtaa cccgagagga ggttcacatg gcacaacctg ccctcgacga cgtcgacctc
     8941 gtcctgaaga cgatggccag gacgatcgtc gaccacgccg acgagttcgc gcacctcgac
     9001 tcgatcgtcg gcgacggaga cttcggcttc tcgctgcgca acggcttcga ggtggtggac
     9061 gcggagtacg acacgttcga tcactccagc gtcggggcga tcctcaagaa gatcggcctg
     9121 gtcatctccg gcaaggtcgg cggcgtcagc gggccgatct ggggcaccgc ctttctgcgt
     9181 gccggcgcat cggccggcga caagaccgag ctgtcgaccg acgacgtcat caccatgctc
     9241 cgcgccgccg tggagggcat caagcagcgc gggcagtcgg acgtcggcga caagaccctc
     9301 ctcgacgcgc tggtcccagc catcgacagc ctcgaggaga gcttccgtga gccgatcgag
     9361 gaccacggcg tggccgccat ccagcgcgcc gccgacgtcg cggtccgcgc ggccgagaac
     9421 accaagggga tgctcgccat gcgcggccgt gccgcgtaca ccggcgagcg gagccgggat
     9481 tccgtggacg ccggcgcgac agcggtcggc gtgatcctcc aggacatcag cgccgcgtgg
     9541 cgcgcgaagt acagcaatgg acaggaggcc cgagcgtgaa gaagttcgtc aacgacccca
     9601 agcagttcgt ccccgagttc ctcgagggca tcgccctggc gagtaacggc gcgctgaagt
     9661 acgagccgaa gtacaacctg atctaccgag cggacatgcc gaagccggac cgggtctcga
     9721 tcatccaggg ctccggctcg ggacacgagc cggcccacac gatgatcgtg ggcccgggga
     9781 tgctggatgc ggcctgcccg ggcgacgtct tcgccgggcc gccggtcgag ttcgtcaccg
     9841 agacctcgaa gctgatggac acgccggccg gcgtgctcta catcgtcaac aactacaccg
     9901 gcgaccgcac ggcgtgggag atggcgaagg agatccagga ggccgagggc cagaagatcg
     9961 gctacctgct ggtcaacgac gacgtggcgg tcaaggactc gacctggacc gtcggtcggc
    10021 gcggcgtggc gggcaacttc ttcgtcatca aggccgcggc cgcgatggcc gacaagggcg
    10081 gctccctgga ggagtgcgtg cgggtggggg agaaggtcat cggcaacgtg cggacgatgg
    10141 gcatcgccct cagctcgtgc acgccgccgg tgcgcggcac gccgctgttc gacatcggcg
    10201 acgacgagat ggagatcggc atcggcatcc acggcgagcc gggtcgcgag cgcgcgaaga
    10261 tgcggccggc gaacgagatc gtggacatcc tgctcgacgc gatcgttccg gacctgccgt
    10321 tcgagtcggg tgacgaggtc gccctcatgg tcaacggcct gggtggcacc ccgatcagcg
    10381 agctgtacct gctgtacggc atcgcgcaca agaagctcga ggagaagggg atcaacgtct
    10441 tccgcagcta cgtcggcgag tactgcacct cgctcgagat ggcgggcgcg cacctgtcgc
    10501 tgctgaaggt cgacgatgag atcaaggagc tgctgctggc tccggcggag gtcgcctacc
    10561 gcgtcttctg atccgatcc
//
LOCUS       DASTYP010000032         6555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_548476, whole genome shotgun sequence.
ACCESSION   DASTYP010000032 DASTYP010000000
VERSION     DASTYP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548476"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFV59_01510"
     CDS             complement(<1..261)
                     /locus_tag="VFV59_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU5324534.1"
                     /translation="MSRTVSTPFAYEVRRTRIRAPGSGYHGPMITLGIDPGTATCGFG
                     VVALEGGRLRLLDAGCIRTSADESDAVRLARLHLAIGELVAHH"
     gene            344..547
                     /locus_tag="VFV59_01515"
     CDS             344..547
                     /locus_tag="VFV59_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324535.1"
                     /translation="MLATPGVLDTLVRDRQRRLRHRPHRAAASSRPLRVRIGHALIQA
                     GANLSGERVERTTRSAHLSPAAR"
     gene            567..1691
                     /locus_tag="VFV59_01520"
     CDS             567..1691
                     /locus_tag="VFV59_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5324536.1"
                     /translation="MSPTRRPSESPQLTIEVAPAYELLQSIAAAIEGDEADTYDVGPE
                     WFAAVRARAGEDLLERIRTFARGESDTYYHLVGLVYDTPAPRDIPTFLDHLRQTDPDE
                     IRLHLVQFYERETRRSTPASVIRAAVGGDADAQDEFLRTSHPDWEPWTAYLRTVLTAD
                     GEAFKDELLAILETWADRVWKPESPTIMPILERDATAKRELIGELPIEQFITTATNGV
                     EFAPRPGISRLVLVPSFVNRPLVTYYEYGELFIIVYPVADESVSAEIDTPPLRLVRLS
                     KALADEKRLRILRALAEGDRSLQELADMFGVAKTTMHHHMIVLRSAGLITVAVGSKNY
                     RLRHETVPDVGALLSGYLGAGPGPSAAPGSPAPARKRAAG"
     gene            complement(1698..2456)
                     /locus_tag="VFV59_01525"
     CDS             complement(1698..2456)
                     /locus_tag="VFV59_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5324537.1"
                     /translation="MSGHSKWSQIKRQKGANDAKRGALFTKLAREIITAARQGGGDPD
                     ANYRLRMAVDKAKANSMPAENIKRAIERATGSGADAEQYEEITYEGIGPASVAVIVAA
                     MTDNRNRTASEVRSIFNKHGGTLSTVAWQFEQKGIISVPLNGRDPDEVTLAAIDAGAA
                     DVGTPDGGAIVVTTEPQELEAVRRALADAGYEADSAELSMEPTTTVEITDEKAARQVL
                     QFVERLEDLDDVQNVYANFDIPADLLEQLEAQVA"
     gene            2645..4720
                     /gene="fusA"
                     /locus_tag="VFV59_01530"
     CDS             2645..4720
                     /gene="fusA"
                     /locus_tag="VFV59_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5324538.1"
                     /translation="MKPTSPDRIRNIALISHGGAGKTSLAEAMLFDAGAINRLGTVEQ
                     GTTTLDWDPDEQKRTQSINLGIATLEHDGVRITIVDTPGYADFQSDVVEALAAVDAAV
                     VVVDASAGVEVGTSEVWKLADARGLPRLVFVNKMDRENANYDATLDQLKAAFGPKIAP
                     VYLPIGSADAFRGYVDVIEEHANVYEDGTPHEVPIPPELEGIEHSRRDALVEAAAEAS
                     DELMMKYLDGEPFSDEEIEAALHKGTREGSVVPVFVGSATKNIGVRELLSMIAKHVPS
                     PAEVGARTTVDGKEIAPDPSGAFVAQVFKATADPFVGRLTYFRVLSGTLSGQGHVYNV
                     NRREEERVGNLLSVQGKDTETLPKVGPGDIAAVAKLSSTRAGDTLVADRAHAVELPAF
                     AFPAPSLQVAVEPESKADLDKLGQALHRLEEEEPCIRIHREEGTGETVLTAMGDAHVD
                     VIVERLKRKFGASVKTATPRVPYRETIRQAARIDNKFKRQTGGHGQYGHVVIEFEPNP
                     GGFEFGDRIVGGVVPKQYIPAVEKGLREAMTEGVLAGYPVVDLKATLVDGSYHTVDSS
                     EMAFKVAASQALKKAFDAGSPTLLEPILEVDIVVPDEYMGDVMGQITAKRGQVLGMDS
                     ADGMQHLRAQVPQAEMFHYATELRSITQGRGRFSWKLDHYAEVPHNIVDKVVAEAAHN
                     GHGSERAGH"
     gene            complement(4759..5007)
                     /locus_tag="VFV59_01535"
     CDS             complement(4759..5007)
                     /locus_tag="VFV59_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324539.1"
                     /translation="MRLGFLTAALAAGLAGAVMAWLVTWLIALVAGDPLGLVGTITII
                     VLAALLVATATFLAEEVALRRTYARMRGTGEPWAYRDR"
     gene            complement(5004..6296)
                     /gene="tyrS"
                     /locus_tag="VFV59_01540"
     CDS             complement(5004..6296)
                     /gene="tyrS"
                     /locus_tag="VFV59_01540"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5324540.1"
                     /translation="MTTPDARAAVLDDLEWRRMIADHTDLDDLRSSMRAGSVTFYGGF
                     DPTAPSLHFGNLVLLVTMRRLQLAGHRPIGLVGGATGLVGDPSGRTSERTLNEREVVA
                     SWVERIRSQVERYLDFDGDNGALIVNNLEWTGEMSAIDWLRDIGKHFSVNRMLAKEAV
                     SARLEAGGISYTEFSYQIMQAVDFLELYRRHGCTLQLGGSDQWGNLTAGVDLIRRAEG
                     ATAHALATPLITRPDGEKFGKSTGSTLWLDPALTSPFAFYQWFLNTDDSVVGTYLRVF
                     SFRSHGEIAALEAVSAERPEAREAQRTLAAEVTELVHGADAAAAAAEASRALFGQAEL
                     QALDADTLAAAFADLPRAPVSTADGLPAVVDLLVATGLADSRGQARRVVTEGGAYVNN
                     ERVTDPAYAPDRSRLLAGRWLLVRRGKRNLAVAEVEPA"
     gene            6344..>6555
                     /locus_tag="VFV59_01545"
     CDS             6344..>6555
                     /locus_tag="VFV59_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324541.1"
                     /translation="MTRRPPPSGRRDAPRSAGRGAPTRRRPGSTTLPRTVLAIFGVAA
                     VGLFVVLMGFYASLTSGLPDVAEIEN"
BASE COUNT         1008 a         2336 c         2276 g          935 t
ORIGIN      
        1 gtggtgggcg accagctccc cgatggcgag gtgcagacgc gccagtcgga cggcatccga
       61 ctcgtcggcc gaggtgcgga tgcacccggc gtccagcagc cgcaggcgcc caccctcgag
      121 cgccacgacg ccgaagccgc acgtcgccgt gccgggatcg atgccgagcg tgatcattgg
      181 cccatggtag ccactcccag gcgcgcggat gcgagttcga cgaacttcgt acgcgaaagg
      241 ggttgacacc gtacgactca tgtcgtacag tacgcacagt tcgatatgta tcggacatcg
      301 gaggcagttg ggacgacagg aaggagttcg taccgtgatg atgatgctgg cgactcctgg
      361 cgtcctggac accctggttc gggatcgcca gcgacggctt cgccaccgac ctcaccgcgc
      421 agccgcatcg agccggccac tgcgcgtccg catcggccat gcgctcatcc aggccggagc
      481 caacctcagc ggtgagcggg tggagcgcac cacccgctcg gcgcacctct cccccgccgc
      541 gcgctgagcg acaggaggaa ccgcccgtgt cgccgacccg acgcccgagc gagtcaccgc
      601 agctgaccat cgaggtcgcc cccgcctacg agctcctcca gtcgatcgcc gccgccatcg
      661 agggcgatga ggccgacacc tacgacgtcg ggcctgaatg gttcgccgcg gtgcgagcgc
      721 gcgccggcga ggacctcctg gagcggatcc ggaccttcgc acggggcgag tccgacacct
      781 actaccacct cgtgggcctg gtctacgaca cgccggcccc ccgcgacatc ccgaccttcc
      841 tcgaccacct tcggcagacg gatccggacg agatccggct ccacctggtg cagttctacg
      901 agcgcgagac gcggcgctca acgccagcca gcgtgatccg cgccgcggtc gggggcgatg
      961 cggacgcgca ggacgagttc ctgcgcacgt cgcaccccga ctgggagccg tggaccgcgt
     1021 acctgcgcac cgtgctcacc gcggatggcg aggccttcaa ggacgagctg ctcgccatcc
     1081 tcgagacctg ggcggatcgc gtctggaagc ccgagtcccc gacgatcatg ccgatcctcg
     1141 agcgcgacgc gaccgccaag cgcgagctga tcggcgagct gccgatcgaa cagttcatca
     1201 ccacggcgac caacggcgtc gagttcgccc cgcgaccggg catcagccgt ctcgtgctgg
     1261 tccccagctt cgtcaaccgc ccgctggtca cgtactacga gtacggcgag ctgttcatca
     1321 tcgtctaccc ggtcgccgat gagagcgtct cggccgagat cgacacgccg ccactccggc
     1381 tcgtgcggct gtccaaggcc ctcgccgacg agaagcggct gcgcatcctg cgcgcgctgg
     1441 ccgagggcga tcggtcgctc caggagctgg ccgacatgtt cggcgtcgcc aagacgacca
     1501 tgcatcacca catgatcgtg ctgcgctctg ccgggctcat caccgtggcc gtggggtcca
     1561 agaactaccg gctgcggcac gagaccgtcc cggacgtcgg ggcattgctc agcgggtacc
     1621 tcggcgccgg gcctggtccg tccgcggcgc cggggtcgcc cgctcctgca cggaagcgcg
     1681 ccgccggctg acggccgcta ggcgacctgc gcctcgagct gctcgagcag gtccgccggg
     1741 atgtcgaagt tggcgtagac gttctgcacg tcgtccaggt cctcgagccg ctcgacgaac
     1801 tgcagcacct ggcgcgcggc cttctcgtcg gtgatctcaa cggtcgtggt cggctccatc
     1861 gacagctcgg ccgaatcagc ctcgtagccg gcgtccgcga gcgcgcggcg caccgcctcc
     1921 agctcctgcg gctccgtcgt cacgacgatg gccccgccgt ccggcgtccc gacatcggct
     1981 gcgcctgcgt cgatggccgc cagcgtcacc tcgtcggggt cccggccgtt gagcgggacg
     2041 ctgatgatcc ccttctgttc gaactgccac gcgaccgtgg agagcgtgcc gccgtgcttg
     2101 ttgaagatgc tgcgcacctc ggatgccgtc cggttccggt tgtccgtcat cgccgccacg
     2161 atgaccgcca cactggccgg gccgatgccc tcgtaggtga tctcctcgta ctgctccgca
     2221 tccgccccgg agcccgtggc gcgctcgatg gcgcgcttga tgttctcggc cggcatcgag
     2281 ttcgccttcg ccttgtcgac ggccatgcgc agccggtagt tggcatcggg gtcgccgccg
     2341 ccctggcggg cagccgtgat gatctcgcga gcgagcttgg tgaacagcgc gccgcgcttg
     2401 gcatcgttcg cgcccttctg gcgcttgatc tgggaccact tgctgtggcc ggacatcgga
     2461 acctctccgc tggcgggtga acgggaggcc gattctacca gcgcgacctt catgagccga
     2521 tgcagcgccc gggggccggg atcggtccgc cacctataat cgggtcgttc ttccggccaa
     2581 cgaaccgccc cgaggcggac ccgcatcggc ctcgggttct cgagcatccc cgcaaggagc
     2641 accgatgaag ccgacctcgc ccgaccgcat ccggaacatc gccctcatct cccacggcgg
     2701 cgccggcaag acgagcctgg ccgaggccat gctcttcgat gccggcgcca tcaaccgcct
     2761 cggcacggtc gagcagggca ccacgacgct cgactgggac ccggacgagc agaagcgcac
     2821 ccagagcatc aacctcggga tcgcgacgct cgagcacgat ggcgtccgca tcacgatcgt
     2881 ggacacgccc ggctacgccg acttccagtc cgacgtcgtc gaagcgctgg ccgccgtgga
     2941 cgccgcggtg gtcgtggtgg acgcgtccgc cggtgtcgag gtcgggacga gcgaggtgtg
     3001 gaagctggcc gatgctcgag ggctgccgcg actcgtgttc gtcaacaaga tggaccgcga
     3061 gaacgcgaac tacgacgcga cgctcgacca gctgaaggcg gcgttcgggc cgaagatcgc
     3121 gccggtctac ctgcccatcg gctccgccga cgcgttccgc ggctacgtcg acgtgatcga
     3181 ggagcatgcc aacgtctacg aggacggcac gccgcacgag gtcccgatcc cgcccgagct
     3241 cgaggggatc gagcactcgc ggcgtgacgc gctggtcgag gccgcggccg aggcgagcga
     3301 cgagctgatg atgaagtacc tcgacggcga gccgttcagc gacgaggaga tcgaggcggc
     3361 gctgcacaag ggcacgcgcg agggcagcgt ggtgcccgtc ttcgtgggct ccgccacgaa
     3421 gaacatcggc gtccgggagc tgctcagcat gatcgccaag cacgttccct cccccgccga
     3481 agtcggtgcg cggacgaccg tcgacggcaa ggagatcgcc cccgacccga gtggggcgtt
     3541 cgtggcgcag gtgttcaagg cgaccgcgga tccgttcgtc ggccgcctca cctacttccg
     3601 ggtcctgtcc gggacgctca gtgggcaggg ccacgtgtac aacgtcaacc gccgcgagga
     3661 ggagcgtgtc ggcaacctcc tgtcggtcca gggcaaggac accgagacgc tgccgaaggt
     3721 cgggcccggg gacatcgcgg ccgtcgccaa gctgagctcg acccgcgccg gagacacgct
     3781 ggtcgccgac cgcgcgcacg ccgtcgagct tccggccttt gcgttcccgg cgccatcgct
     3841 gcaggtcgcg gtcgagccgg agtcgaaggc cgacctggac aagctcggcc aggcgctgca
     3901 tcggctcgag gaggaggagc cgtgcatccg gatccatcgc gaggagggca ctggcgagac
     3961 cgtgctcacc gccatgggcg acgcccacgt cgacgtcatc gtggagcgcc tgaagcgcaa
     4021 gttcggcgcc tccgtgaaga ccgcgacgcc ccgcgtcccg taccgcgaga cgatccgcca
     4081 ggcggcgcgc atcgacaaca agttcaagcg ccagaccggc gggcacgggc agtacgggca
     4141 cgtggtgatc gagttcgagc ccaaccccgg cgggttcgag ttcggcgacc ggatcgtcgg
     4201 tggcgtggtg ccgaagcagt acatccccgc cgtcgagaag ggcctccgcg aggccatgac
     4261 cgagggcgtg ctggccggct acccggtggt cgatctcaag gccaccctgg tcgacggctc
     4321 gtaccacacc gtcgacagct cggagatggc cttcaaggtc gcggcctcac aggcactgaa
     4381 gaaggcgttc gacgccggga gccccacgct gctggagccg atcctggagg tcgacatcgt
     4441 ggtgccagac gagtacatgg gcgacgtgat gggccagatc accgccaagc gtggccaggt
     4501 gctcggcatg gactccgcgg acgggatgca gcacctgcgg gcccaggtgc cacaggccga
     4561 gatgttccac tacgccacgg agctgcggtc gatcacccag ggccggggcc gcttcagctg
     4621 gaagctcgac cactacgccg aggtgccgca caacatcgtg gacaaggtgg tggccgaggc
     4681 ggcgcacaac ggccacggct cggagcgcgc cggccactga cctgccgtgc ggcgtggcac
     4741 gtgctgcgcg ggcggccctc agcggtcgcg gtaggcccag ggctcgcccg tgccgcgcat
     4801 gcgcgcgtac gtgcgccgca gggcgacctc ctcggccagg aaggtcgccg tcgccacgag
     4861 caacgcggcg agcacgatga tggtgatcgt tccgaccagg cccagcggat cgccggccac
     4921 gagcgcgatc agccaggtca cgagccaggc catcaccgcg ccggccaggc cggcggccag
     4981 cgccgcggtg aggaacccga gcctcatgcc ggttccacct ccgcaacggc gaggttgcgc
     5041 ttcccacgac ggaccaggag ccatcggccg gccagcaggc gggagcggtc gggcgcgtac
     5101 gccgggtccg tgacccgctc gttgttgacg taggcaccgc cctccgtcac cacccgccgc
     5161 gcctggccac ggctgtccgc gaggccggtc gccacgagca ggtcgacgac ggcgggcagg
     5221 ccgtccgccg tcgacaccgg tgcgcggggc agatcggcga aggcggcggc cagcgtatcg
     5281 gcgtcgagcg cctggagctc cgcctggccg aagagggcgc ggctggcctc ggccgcggcg
     5341 gcggccgcat cggcgccgtg caccagctcg gtcacctccg cggccagggt gcgctgcgcc
     5401 tcacgcgcct cggggcgctc ggccgagacg gcctcgagcg cggcgatctc gccgtgagag
     5461 cggaagctga agacgcgcag gtaggtcccc accaccgagt cgtcggtgtt caggaaccac
     5521 tggtagaagg cgaacggcga ggtcagcgcg gggtcgagcc agagggtcga gccggtggac
     5581 ttgccgaact tctcgccatc ggggcgggtg atcagcggcg tggcgagcgc gtgcgccgtc
     5641 gcgccctccg cgcggcggat caggtcgacg ccggcggtca ggttgcccca ctggtcgcta
     5701 ccgccgagct ggagcgtgca gccgtggcga cggtagagct ccaggaagtc gacggcctgc
     5761 atgatctggt agctgaactc ggtgtagctg atcccgccgg cctcgaggcg cgcgcttacg
     5821 gcctccttcg ccagcatgcg gttgacgctg aagtgcttgc cgatgtcccg cagccagtcg
     5881 atggccgaca tctcgccggt ccactccagg ttgttgacga tcagcgcgcc gttgtcgccg
     5941 tcgaagtcga ggtaccgctc cacctgggag cgaatgcgct ccacccagga cgcgacgacc
     6001 tcgcgctcgt tgagcgtgcg ctcgctggtc cgcccgctcg gatcccccac cagcccggtc
     6061 gcgccgccga ccagcccgat cggccggtgg ccggccagct gcaggcgacg catggtgacc
     6121 agtaggacga ggttgccgaa gtgcaggctc ggcgcggtgg gatcgaaccc gccgtagaag
     6181 gtgaccgatc ccgcgcgcat cgacgagcgc aggtcgtcca gatcggtgtg gtcggcgatc
     6241 attcgccgcc actcgaggtc gtcgaggacg gcggcgcgcg cgtcgggggt cgtcatcggc
     6301 ggttgaggat agcaccgaac atccgtgcta ccctgcctcg cggatgaccc gacgaccacc
     6361 cccgagcggc cgtcgcgacg cgccccgctc cgccggccgt ggagcgccga cgcggcgccg
     6421 gcccggctcc accaccctcc cccgcaccgt gctggcgatc ttcggcgtcg cggccgtcgg
     6481 gctgttcgtg gtgctgatgg gcttctacgc gtcgctgacc agcggcctgc ccgacgtggc
     6541 ggagatcgag aactt
//
LOCUS       DASTYP010000033         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_621263, whole genome shotgun sequence.
ACCESSION   DASTYP010000033 DASTYP010000000
VERSION     DASTYP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621263"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /gene="dnaX"
                     /locus_tag="VFV59_01550"
     CDS             <1..1659
                     /gene="dnaX"
                     /locus_tag="VFV59_01550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU5324542.1"
                     /translation="PLMPAPQMPTDVVTDAVPQPHRALYRRFRAQRFAEVIGQDPIVT
                     TLRRAIASDRLAHAYLFVGPRGTGKTSTARILAKAINCTALAEDGEPCDACPSCTSIR
                     DGRALDVIEIDAASHGLVEDARDLVMRALTAPSELRKRVYIIDEVHMLSTHAFNALLK
                     LIEEPPDHVVFILATTDTHKVPPTIISRTQRHDFRRLADAIIAGKLARICATEGVEAD
                     ADALDLVARLADGGMRDAESILDQVLAYVAGRVTAAEVREAVGLADDEAIVALVDAYV
                     AGDAAGALGRLADLADAGRDLAQVAAQAEGEARRRLLASASRPAEAARLAPILRTLAE
                     AAGAGAREGRARLLMELLAVESATASRAAPAPVAAAEAPAPARAPVEIRPAAATQTIP
                     KPAAAPARPDDGVAAPRAAARTTPAPAPVAEPTAGASGDLAAIRGRWADVVERATPVI
                     KPLLKECRPVARDGARLTLAFPEGRDFMRSRISQRSAAIEALLTEMFGGAFAIECVAT
                     NVELEPLTVEQAIAPSDDDPEARALLEGVLKITGGELVDAPEVR"
     gene            1694..2020
                     /locus_tag="VFV59_01555"
     CDS             1694..2020
                     /locus_tag="VFV59_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5324543.1"
                     /translation="MNIGQIAKMAQQMQTQLAQAQEELKETTLEATAGGGAVRVVITG
                     AQEVRSVEIDPSAVDPDEVEMLQDLVMTAVNEAIGRSKELERERMAGIAGGMGLPGMP
                     GLPGFG"
     gene            2028..2639
                     /gene="recR"
                     /locus_tag="VFV59_01560"
     CDS             2028..2639
                     /gene="recR"
                     /locus_tag="VFV59_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU5324544.1"
                     /translation="MAGLIAPVARLIEEFSKLPGIGTKTAQRLTYHVLRGSSDDARGL
                     AAALVAIKEQVAYCSVCCNITEAGVDPCDICADPRRDAAHICVVEEPLDVLAIERTSE
                     YRGRYHVLHGAISPIDGIGPERLRTRELIARVAAGGVEEVILATNPNLEGEATAMYLA
                     DLLAPHVPSVTRIARGLPVGGDLEYADDVTLIRSLQGRRAIER"
     gene            2921..>3275
                     /locus_tag="VFV59_01565"
     rRNA            2921..>3275
                     /locus_tag="VFV59_01565"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          518 a         1186 c         1121 g          450 t
ORIGIN      
        1 cccctgatgc ccgcgcccca gatgcccacc gacgtcgtca ccgacgccgt cccgcagccg
       61 caccgagcgc tctaccgccg ctttcgcgca cagcggttcg cggaggtcat cggccaggac
      121 ccgatcgtga ccaccctgcg acgtgccatc gcgtcggatc ggctggcgca cgcctacctg
      181 ttcgtcggtc cgcgcggtac cggcaagacc tcgacggcgc gcatcctggc caaggcgatc
      241 aactgcaccg cgctcgccga ggacggtgag ccgtgcgatg cctgcccgtc gtgcacgtcg
      301 atccgcgacg gccgcgcgct cgacgtgatc gagatcgacg ccgcctccca cggcctggtg
      361 gaggacgccc gtgacctcgt catgcgcgcg ctcaccgctc cctcggagct gcgcaagcgg
      421 gtctacatca tcgacgaggt ccacatgctc agcacgcacg ccttcaacgc gctgctgaag
      481 ctcatcgagg aaccgcctga tcacgtcgtc ttcatcctgg ccacgaccga tacgcacaag
      541 gtcccgccga ccatcatcag ccgcacgcag cgccacgact tccgtcgcct ggccgatgcg
      601 atcatcgccg gcaagctggc ccgcatctgc gccacggaag gcgtcgaggc cgatgccgat
      661 gcgctggacc tggtcgcccg cctggccgac gggggcatgc gcgacgccga gtcgatcctc
      721 gaccaggtgc ttgcctacgt cgccgggcgc gtgaccgccg ccgaggtgcg cgaggcggtc
      781 ggcctggccg acgacgaggc catcgtcgcg ctggtggacg cctacgtggc cggggacgcg
      841 gccggcgcgc tcgggcgcct ggccgacctg gccgatgccg gccgggacct ggcgcaggtc
      901 gccgcgcagg ccgagggcga ggcgcgccgc cgcctcctcg cctccgccag ccgtccggcc
      961 gaggccgctc gactcgcgcc gatcctgcgc acgctcgccg aggccgccgg ggccggcgcg
     1021 cgagagggcc gtgcccgcct cctcatggag ctcctggcgg tcgagtccgc caccgcctcc
     1081 cgcgctgctc ctgcgcccgt ggccgccgca gaagcaccgg ctcctgcccg cgcgccggtc
     1141 gagatccgcc cggcggctgc cacgcagacc atcccgaagc cggcggccgc accggccaga
     1201 ccagacgacg gcgtcgcagc tccacgtgcc gccgcgcgga caaccccggc gcctgcgccg
     1261 gtcgcggagc ccaccgccgg cgcgtccggg gacctcgccg ccattcgcgg ccgctgggcg
     1321 gacgtggtgg agcgcgccac gccggtcatc aagcccctgc tcaaggagtg ccgcccggtg
     1381 gcgcgtgatg gcgcgcgact caccctcgcc ttccccgagg gccgcgactt catgcggtcc
     1441 aggatctcgc agcgctcggc cgccatcgag gcgctattga ccgagatgtt cggtggcgcc
     1501 ttcgccatcg agtgcgtcgc caccaacgtg gagctcgagc cgctgacggt cgagcaggcc
     1561 atcgcgccat ccgacgacga ccccgaggcg cgcgcgctgc tcgagggcgt cctcaagatc
     1621 accggcgggg agctcgtcga cgcccccgag gtccgctgac ccccgtccca tccccaacac
     1681 ggagaccgat tccttgaaca tcggccagat cgccaagatg gcgcagcaga tgcagaccca
     1741 gctcgcgcag gcgcaggagg agctgaagga gacgaccctc gaggcgaccg ccggtggcgg
     1801 cgcggtgcgg gtcgtgatca ccggcgcgca ggaggtgcgc agcgtcgaga tcgacccgtc
     1861 ggcggtcgac cccgacgagg tggagatgct ccaggacctg gtcatgaccg ccgtgaacga
     1921 ggcgatcggg cgcagcaagg agctcgagcg cgaacgcatg gccggcatcg ccggcggcat
     1981 gggactgccc ggcatgcccg ggctgccggg gttcggctga gccctcggtg gccgggctca
     2041 tcgcgccggt cgcgcgcctg atcgaggagt tcagcaagct ccccggcatc ggcacgaaga
     2101 ccgcccagcg tctcacgtac cacgtcctgc gcggatcgtc ggatgacgcc cgcgggctcg
     2161 cggctgccct ggtggccatc aaggagcagg tggcgtactg cagcgtgtgc tgcaacatca
     2221 ccgaggccgg cgtcgatccg tgcgacatct gcgccgatcc ccgccgcgac gcggcgcaca
     2281 tctgcgtggt cgaggagcca ctcgacgtgc tggccatcga gcgcacctcg gagtaccgcg
     2341 gccgctatca cgtcctgcac ggcgccatca gccccatcga cggcatcggt ccggagcgac
     2401 tgcggacgcg cgagctgatc gcgcgggtcg ccgccggcgg cgtggaggag gtgatcctcg
     2461 ccaccaaccc gaacctggag ggcgaggcga cggccatgta cctcgccgac ctgctcgccc
     2521 cacacgtgcc gagcgtgacg cgcatcgccc gcggcctgcc agtcggcggt gatctcgagt
     2581 acgccgacga cgtgaccctg atccgatcgc tccagggacg gcgcgccatc gagcgctgag
     2641 gctcgcttga cggcctcgaa cgggtgtcct atagtccgtc atcgcgccgg tcgacaggcc
     2701 ggccgatgtg ccacgaacgg cgatcgaacc cgatcgcgcc gcgtgaccat cacgagttca
     2761 cctcaacagg agactcgaat cgaacgacgc cgcgctcgcg gaagcaggcc ggcaacgtgg
     2821 cttaccagct gaagagtgac aggaatccac acgagtcagc gggacaacgt cgaactcgtt
     2881 aaccggtgag gaggcgcaag ccaatccact ggtttcgttt tgtacggaga gtttgatcct
     2941 ggctcaggac aaacgctggc ggggtgcgtg agacatgcaa gtcgaacgag gagcctccga
     3001 gcttgcttgg aggcttccta gtggcggacg gctgagtaac gcgtaggtga cctgcccgga
     3061 agtggggaat aactgcccga aagggtggct aataccgcat gtggtcggcg attcggttcg
     3121 tcgtctaaag gagcaatccg cttccggagg ggcctgcgtc cgattagcta gttggtgggg
     3181 taacggctca ccaaggcgac gatcggtagc tggtctgaga ggatgatcag ccagactggg
     3241 actgagaacg gcccagactc ctacgggagg cagca
//
LOCUS       DASTYP010000034         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1204157, whole genome shotgun sequence.
ACCESSION   DASTYP010000034 DASTYP010000000
VERSION     DASTYP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204157"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="VFV59_01570"
     CDS             <1..1618
                     /locus_tag="VFV59_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5324545.1"
                     /translation="EVRAVLSGGSLANDAVLLNQAGSWTVQGDPTEAAFLVAERKLGT
                     TEQRMQRFRRRGEIPFSSERKLMSTLEEDIARAGEVVVMTKGAPDVLLARCTHERVGS
                     EAVPLTDARRTAIAASVDDLADAALRTLAVAYRPLGTLEAPAADEALERELVFAGVVG
                     MIDPPRTEARDAIAEAMGAGIRVVMITGDHARTASRVAGDLGLAGDAAVVGGQELETL
                     SDAELRERARSASVYARVAPEHKLRIVDALQAEGQIVAMTGDGVNDAPALKAADIGVA
                     MGITGTAVSKEAADMILADDNFATIVAAVREGRAIFANIRRFLRYLLSSNAGEVLTMF
                     LGVVGASVIGLTQVGEGLAAPLLATQILWINLLTDSAPALALGFEPPPADVMRRPPRR
                     LTDRVIDAEMQMDVVLVGLTMAVATLLALDIFLPGGLIAGAGSLDEARTAAFTVLVLA
                     QLYNAFNARSDHQSAFRGLFTNWRLFGAVALSLTLQVLVVHVPFLNHAFGTVPLSGVD
                     WLLCLVLASSVLWVAELRKVVVRRRARTAA"
     gene            1622..2656
                     /locus_tag="VFV59_01575"
     CDS             1622..2656
                     /locus_tag="VFV59_01575"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5324546.1"
                     /translation="MRLDSIVHAASALAREQSRTTPGGYVAMAVGTASEALGSVAFVR
                     GEAVEPAPRSPIASVTKPITATAVMQLVDTGLVDLDTPIRSVIPELAARPVAQAATIT
                     LRHVLSHTSGLHDVSDKQLHELPPSPDAMLAAVCRQRLRFAPGTAFQYASEPWYLLSA
                     LIERISGLAYPAYLSDRVFGPLGMNVTSFDPADARRPSLPPQGSFALPGLSTHEVAVR
                     LRSLEMPGGGLWSTADDLLRFARAMLGGAELDGTRVLGGRTLEAMTRLQTAGLADARS
                     GAAVAYGLGWGLRPGLGASEGAYAHSGATGCLLVVDPERDLAIVYLRNRWAAPMEDAN
                     RAVEAVLAAS"
     gene            2719..>3825
                     /gene="argH"
                     /locus_tag="VFV59_01580"
     CDS             2719..>3825
                     /gene="argH"
                     /locus_tag="VFV59_01580"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5324547.1"
                     /translation="MARPLWGSRVQAPLTPTAARISSSVDIDRPLAAYDVAATRAHVG
                     ELHRLHLVDEGGRDRLLAALDEVAAELEAGTFAWDEAAEDVHMNVERAIRARTGDELA
                     GQLQAGRSRNEEIVADERLWLKAALHRLDDRLLSLQRTLLQRAEEEIETVMPAHTHTQ
                     PAQPVLLAHHLHAYVEIFGRNRQRLTDAAARLDRSPAGSGAAVGSGLPIDRVTVADAL
                     GFSDITANSLDAVASRDYALEVVADAAITFTDLGRLAADLVLWSTPYFAFARIPDGYA
                     TGSSMLPNKRNPDIAELIRGRSARATAALTGMLAILHGLPLGYHRDLQELRGPMLETI
                     ASLELCLAAADEMVAGVSFDREAMADAAQQGHALA"
BASE COUNT          507 a         1396 c         1369 g          553 t
ORIGIN      
        1 cgaggtgcgc gccgtcctgt ccggcggctc gctggccaac gacgcggtcc tgctgaacca
       61 ggccggcagc tggacggtgc agggcgatcc gaccgaggcc gcgttcctcg tcgcggagcg
      121 caagctgggg acgacggagc agcggatgca gcgattccgg cgccgcggcg agatcccgtt
      181 cagcagcgag cgcaagctca tgagcacgct ggaggaggac atcgcccgcg ccggcgaggt
      241 cgtcgtcatg acgaagggcg cgccggacgt gctgctggcg cgctgcacgc acgagcgggt
      301 cggctcggag gccgtcccgc tcaccgacgc tcgccgcacg gcgatcgcgg cgtcggtcga
      361 cgacctggcg gatgcggcgc tgcggacgct ggcggtggcg taccggccgc tcggcaccct
      421 cgaggcgccc gcggcggacg aggcgctcga gcgcgagctg gtcttcgccg gcgtggtcgg
      481 catgatcgac cctccgcgca ccgaagcgcg cgacgccatc gccgaggcca tgggagccgg
      541 catccgcgtg gtgatgatca ccggcgatca cgcgcggacc gccagccgcg tggccggtga
      601 tctcgggctg gccggcgacg cggcggtcgt cggaggccag gagctggaga ccctctccga
      661 cgccgagctg cgggagcggg ctcggagcgc gagcgtgtac gcgcgcgtgg cgccggagca
      721 caagctgcgc atcgtcgacg cgctgcaggc cgagggacag atcgtcgcga tgaccggcga
      781 cggggtcaac gacgcgccgg cgctcaaggc ggcggacatc ggcgtggcga tgggcatcac
      841 cgggacggcg gtcagcaagg aggccgccga catgatcctg gcggacgaca acttcgcaac
      901 gatcgtggcg gccgttcgcg agggccgcgc catcttcgcc aacatccggc ggttcctgcg
      961 gtacctgctc tcgtcgaacg cgggcgaggt gctgacgatg tttctcgggg tcgtcggcgc
     1021 atcggtcatt gggctcacgc aggtcggcga gggcttggcg gcgccgctgc tggccaccca
     1081 gatcctgtgg atcaacctgc tcaccgactc agcgccggcg ctcgccctgg gcttcgagcc
     1141 gccgccggcc gacgtcatgc gccgtccacc acgacgcctc accgaccggg tcatcgatgc
     1201 cgagatgcag atggacgtcg tgctggtcgg cctgaccatg gccgtcgcga ccctgctggc
     1261 gctggacatc ttcctgcccg gtggcctgat tgccggggcc gggtcgctgg acgaggcgcg
     1321 gacggcagcg ttcacggtgc tcgtgctggc acagctgtac aacgccttca acgcgcgatc
     1381 cgaccatcag tcggccttcc gcgggctctt caccaactgg aggctcttcg gcgccgtggc
     1441 actctcgctg acgctccagg tcctggtcgt tcacgtcccg ttcctgaacc acgcgttcgg
     1501 gacggtgccg ctgtccgggg tggactggct gctgtgcctg gtcctcgcca gctccgtgct
     1561 gtgggtggcc gagctgcgca aggtcgtcgt gcggcggcgc gccaggacgg ccgcctgacc
     1621 gatgcgtctc gattccatcg tccacgccgc atccgcgctg gcccgcgagc agtcgcggac
     1681 gacgcccggc gggtacgtgg cgatggcggt gggcaccgct tcggaggcgc tcggatcggt
     1741 ggcgttcgtg cgcggtgagg ccgtggagcc ggccccgcgg tcacccatcg cctcggtgac
     1801 gaagccgatc acggcgacgg cggtgatgca gctggtcgac accgggctgg tcgacctcga
     1861 cacgccgatc cgatccgtga tcccggagct ggccgcacga cccgtcgccc aggcggcaac
     1921 gatcaccctt cggcacgtcc tgtcgcacac gtccggcctg cacgacgtgt ccgacaagca
     1981 gctgcacgag cttccgcctt caccggacgc gatgctcgcg gccgtgtgcc ggcaacgcct
     2041 acggttcgcg cccggcacgg cgttccagta cgcgtcggag ccgtggtacc tgctgtccgc
     2101 gctgatcgag cgcatctccg ggcttgcata tccggcgtac ctgtccgatc gcgtcttcgg
     2161 gccgctgggc atgaacgtaa cgagcttcga cccggccgat gcgcgccgcc cctcgctgcc
     2221 gccgcagggc tccttcgcgc ttcccgggct gtccacgcac gaggtggccg tccggctgcg
     2281 ttccctcgag atgcccggcg gcggtctgtg gtcgaccgcg gacgacctgc tccgcttcgc
     2341 ccgggccatg ctcggaggtg cggagctgga cggtacccgc gtcctcgggg gccgaacgct
     2401 cgaggcgatg acccggctgc agaccgccgg gctggccgat gctcggagcg gagcggcggt
     2461 cgcatacggt ctgggctggg gactgcgacc cggactcggc gccagcgaag gggcgtacgc
     2521 gcactccggc gccaccggct gcctcctggt cgtggatccg gagcgtgact tggcgatcgt
     2581 gtacctccgt aaccggtggg ccgcgcccat ggaggacgcc aaccgtgcgg tcgaggccgt
     2641 cctcgcggct tcctgatcca ctccggacga gggccgcggt tgcatgacaa tgcggtgtga
     2701 gtgcatactc atgcgtccat ggcacgacca ctgtggggca gccgggtcca ggcgcccctg
     2761 actccgaccg ccgcccgtat ctcgagctcc gtcgacatcg accgcccgct ggccgcgtac
     2821 gacgtcgccg cgacgcgcgc ccatgtcggg gagctgcatc ggctgcacct ggtcgacgag
     2881 ggagggcggg accgcctcct ggccgcgctc gacgaggtcg cggccgagct cgaggccggc
     2941 acgttcgcct gggacgaggc cgccgaggac gttcacatga acgtcgagcg cgccatccgc
     3001 gcgcgcaccg gcgacgagct ggccggccag ctccaggcgg gccgcagccg caacgaggag
     3061 atcgtcgccg acgagcggct ctggctgaag gccgcgctcc atcggctgga cgatcggctg
     3121 ctgtcgctgc agcgcacgct cctccagcgc gccgaggagg agatcgagac ggtcatgccg
     3181 gcgcacacgc acacccagcc ggcccagccg gtcctcctcg cccaccacct gcacgcctac
     3241 gtcgagatct tcggccggaa ccggcagcgc ctgaccgatg cggccgcccg cctggaccgc
     3301 tcccccgcgg ggtcgggggc ggccgtgggc agcggcctcc ccatcgaccg cgtcacagtc
     3361 gccgacgccc tgggcttctc cgacatcacg gccaacagcc tcgatgcggt ggccagccgc
     3421 gactacgccc tcgaggtcgt ggcggacgcc gccatcacct tcaccgacct cggtcggctg
     3481 gccgcggatc tggtgctgtg gtcgacgccc tacttcgcct tcgcccgcat cccggatggc
     3541 tacgccacgg gctcctcgat gctcccgaac aagcgcaacc ccgacatcgc cgagctgatc
     3601 cgcggacgct cggcccgggc cacggcggcg ctcacgggca tgctcgcgat cctccacggc
     3661 ctgccgctcg gctaccaccg cgatctccag gagctgcgcg ggccgatgct cgagacgatc
     3721 gcgtcgctcg agctctgcct cgcggccgcc gacgagatgg tcgccggcgt cagcttcgac
     3781 cgcgaggcca tggcggacgc cgcgcaacag ggccacgcgc tcgcc
//
LOCUS       DASTYP010000035         8236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1568645, whole genome shotgun sequence.
ACCESSION   DASTYP010000035 DASTYP010000000
VERSION     DASTYP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568645"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFV59_01585"
     CDS             <1..306
                     /locus_tag="VFV59_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324548.1"
                     /translation="HCVHGKSASVEEILDWRPYDYFTIQNDVRMPLLRPFRTTYELED
                     TPTGTRVTEYTAAGGGFGQRFLLRSASRLMAGQLARGQQALQAAAAKAVEPPSQAPE"
     gene            687..971
                     /locus_tag="VFV59_01590"
     CDS             687..971
                     /locus_tag="VFV59_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324549.1"
                     /translation="MLGFGASAGGAFLLGAILLAELDRRWLWIAGAMLQLMVVWGYFA
                     VAPQRDPAFEVWGLTLRVIQLPLLAALVWLALRPSDPHADALVHLRRTDR"
     gene            985..1530
                     /locus_tag="VFV59_01595"
     CDS             985..1530
                     /locus_tag="VFV59_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU5324550.1"
                     /translation="MKVLVAYATRHGATAGIAERIGSKLREHGLDATVLPAKDVRDVR
                     EYDAFVIGSAAYMYHWLKDATDFVHRHRAALKATPVWIFSSGPLGTDLVDKDGKDVRA
                     TAAPKEFAELRATLDPRGEHIFWGAYDPDAPAIGMGEWMMKKMPGAREALPNGDFRNW
                     AEIEAWATEIAEALVRVAAPA"
     gene            complement(1587..2291)
                     /locus_tag="VFV59_01600"
     CDS             complement(1587..2291)
                     /locus_tag="VFV59_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5324551.1"
                     /translation="MVEPTGASEPTHEPLRVLVVDNDPHVAELVSAILTDEGYEVATL
                     AQADHGSIAAAVGRIEPDCILLDSAEGPEFGGSWAEAAYLSRRARPVPTVMFTAHAED
                     VLEAQEHSSDRALRAEFAGIVPKPFMLDQLLDAVASAAGGSLRFDRSEEGDRGRTAAL
                     VADLEGAGATDIRTSSRREWATFVSPSDHRIYQLYWWQRLGRYMLGRYDDDARMELIG
                     QYFERSEAIAAATPSH"
     gene            2803..3963
                     /locus_tag="VFV59_01605"
     CDS             2803..3963
                     /locus_tag="VFV59_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324552.1"
                     /translation="MAKNDKGGEGRADAAQRRKVTHGYDRGPKQKSQKRPRRTLEEKL
                     RRYESHAEGYEFIPALMHSLQRVVEGPPKGTETAEDRRLAQALKEAGATELIARAVRA
                     HNQIPMELKRQAFSPRLLTRPLDEPVGAEETAAIVQRAATMVNVSVAAITPSRLAPAA
                     ADPKHRGMCCCPDTTTPPRDPEPPKPPPNQYELTFQKLYCVDESDPEWWGSDEPYVVF
                     GIITEEMAESGTAAQAVATPVYEDVDDGDTRPSSGDQALRLFGFTGPRAIASSVLISA
                     TCFEHDLGDVSATTDAVRAALTAVATKAAAAGGYVGWIVAGVAVVGIGVSYLVDLIGA
                     DDKIDGSQVMSLTQADADARTASVNPSVFPPLHFDGGDGDGIYDVYLKLRRV"
     gene            4324..4662
                     /locus_tag="VFV59_01610"
     CDS             4324..4662
                     /locus_tag="VFV59_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324553.1"
                     /translation="MRPLPHVPRVTLAIAALAFAAGYAVAAWRSSPQMATGTAYAAED
                     QISVEADDFTYAIPVDVIWTDANGTVHHGDRPGCLPPSGDPITDVRFAYVEASIEGAS
                     WRPVVWVDCR"
     gene            4673..5101
                     /locus_tag="VFV59_01615"
     CDS             4673..5101
                     /locus_tag="VFV59_01615"
                     /inference="COORDINATES: protein motif:HMM:NF039475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6508 domain-containing protein"
                     /protein_id="HEU5324554.1"
                     /translation="MTDEHLRRIGQHLPTLERPGFRFGEWVPSRRDDGGALHLGWYEP
                     SEAAESFLADVRAWIEPFAWPAWAESAEGQRLLGHPDAVASASAEDLRRLLIVYVRAE
                     RFGDGTLEAAFESGMLTAIVRRAAALAGEADAPGPETAGR"
     gene            complement(5129..5623)
                     /locus_tag="VFV59_01620"
     CDS             complement(5129..5623)
                     /locus_tag="VFV59_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324555.1"
                     /translation="MKTTAKLLATLATISLLLAACAAGPSSPTSPAATPTPTAPSASD
                     GDDPTDEPTGSPAPVGTLTVADGAVIDGPGTPLAEALAGDLSQPVLVRGTLFLDADGN
                     VYLADSLTDATAPTFGDTRIRVENYPTSGPTWDISQGELIGLQEANGILFFEDTKLYG
                     TISS"
     gene            6074..6481
                     /locus_tag="VFV59_01625"
     CDS             6074..6481
                     /locus_tag="VFV59_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5324556.1"
                     /translation="MPYEPDPRVDAYIDALPEWQQRICREVREIAHEADPELTETIKR
                     TVQPYFVLDGNVCALQATKDHVNVFLYDGGIVPDPEGIVTHGHGNETGRQIAIHEGEP
                     INRSALLAYLRQIIADNRAGGWRRLKAARGESR"
     gene            complement(6478..7026)
                     /locus_tag="VFV59_01630"
     CDS             complement(6478..7026)
                     /locus_tag="VFV59_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU5324557.1"
                     /translation="MEMTENPRRSSLDDDTPGSQGEVDPGSEGDFRGEPMKGATPNGD
                     QADANKALVRRFIDEVFVQGRPESVEQLVAPDFVSHTWGMTEDGRAKLRATTERIRGT
                     LTDIAFDVEDVLAEGDRVAVRLTASATPTGDFLGVPAAGKRYRIGEMHWFRLADGRIA
                     EHWHQYDRLGMMQQLGALPAKP"
     gene            complement(7092..>8236)
                     /locus_tag="VFV59_01635"
     CDS             complement(7092..>8236)
                     /locus_tag="VFV59_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324558.1"
                     /translation="KKLRLGASLVAFLVALLPAATPVAAASCNGASHESSLQAGTVTP
                     STVTTGEAVTFSVTYTDTAACAPSKVVVSISGVGTFPLAGSGTDYASGVTYSASLNLP
                     VGTRAYSFTVTSGNGRGTTTVTLTGVSPTNVVVAPPAPTATPVPAPVATPVAAVPPPP
                     PPPPVAAPAAVAPAPGAPTAPPPPPPTTPPSAAPVAPSVVQPPAAASPSPAPTKSAAL
                     LAPMHDSWVGGPVAAAPASSTAPQPRDPEPLGTFLAEQLPLTAGTFVATTAGGLTLFF
                     LLIRRRRPTDPEPQPAAVTESAASATLARLDQDIPPATSSDAPIKLNLPPIRELVPPL
                     DPYLLEDPDERVGPSPDEAGIPRWLRPSVRYARTGPIELRQRNWKA"
BASE COUNT         1198 a         2850 c         3040 g         1148 t
ORIGIN      
        1 cactgtgtgc acggcaagag cgcgagcgtc gaggagatcc tcgactggcg cccgtacgac
       61 tacttcacga tccagaacga cgtccggatg ccgctcctgc gccccttccg caccacgtac
      121 gagctggagg acacgcccac cggcacgcgg gtcacggagt acacggcggc tggtggtggc
      181 ttcggccagc gattcctgct gcgctccgcc tcacgcctca tggcggggca gctggctcga
      241 gggcagcagg cgctccaggc ggcggcggcg aaggctgtcg aaccgccatc gcaggcgcct
      301 gagtagcggc cgctcccgcc cccgcgagtt gaggacggcg gcccgcggca cggccgacgg
      361 atcggccacc gcgacgctca acgggcacgc cgcgttgcgg ttaaggctcg gcattggccg
      421 atgcagcggc cgccaccagg ttgtcgcgag gtgaccaacg gccccgccga cgggggcgga
      481 gcggccgttc gccgccaccc aagggccggc gtacaacatg ggtgccacgg aggaggggcg
      541 gaacccgatg gaggacggag ccatgcggcg tcgcaccatc cgctacgtcg cggccgggct
      601 ggcggccgtg atggcggtca tctacttcct catcggcctg ggcgtgctgc aggtggtcga
      661 gggccaggcc agccagcgat ccgtcgatgc tcggcttcgg cgcatcggcg ggtggcgcgt
      721 tcctgctcgg cgcgatcctc ctggcggagc tcgaccgccg atggctctgg atcgcgggtg
      781 ccatgctgca gctgatggtc gtgtggggct acttcgcggt cgcgccgcag cgagatccgg
      841 cgttcgaggt atggggcctc acgctccgcg tgatccagct gccgctgctg gcggcgctcg
      901 tctggctggc gctgcgcccg tcggacccgc acgccgatgc gctcgtccac ctgcgccgca
      961 ctgaccgttg aaaggagact cgagatgaag gtcctcgtcg cctatgcgac gcggcatggc
     1021 gcgaccgcgg gcatcgcgga gcgcatcggt tcgaaactgc gtgagcacgg gctggacgcg
     1081 accgtgctgc ccgccaagga cgtccgcgac gtgcgtgagt acgacgcgtt cgtcatcggt
     1141 tcggcggcgt acatgtacca ctggctgaag gacgcgaccg acttcgtgca ccgccaccgg
     1201 gccgcgctga aggcgacccc ggtgtggatc ttcagctccg gcccgctcgg caccgacctg
     1261 gtcgacaagg acggcaagga cgtgcgcgca acggccgcgc cgaaggagtt cgccgagctg
     1321 cgcgcgacgc tggatccccg aggcgagcac atcttctggg gtgcctacga ccccgatgcg
     1381 ccggccatcg gcatgggcga gtggatgatg aagaagatgc ccggcgcgcg ggaggcgctg
     1441 cccaacggcg acttccggaa ctgggcggag atcgaggcct gggcgacgga gatcgccgag
     1501 gcgctcgtgc gggtggccgc ccccgcctga ccgtccgcgg caccggggtg gtagccggtc
     1561 gaaccggacc gcgccgcggg acggcgctag tgcgacggcg tggcggccgc aatcgcctcg
     1621 ctgcgctcga agtactgacc gatgagctcc atgcgggcgt cgtcgtcgta gcggccgagc
     1681 atgtatcggc ccaggcgctg ccaccagtac agctggtaga tgcggtggtc gctgggcgac
     1741 acgaacgtcg cccactcgcg tcggctgctg gtgcggatgt cggtcgcgcc cgctccctcc
     1801 aggtccgcca ccagcgccgc ggtgcggccg cggtcgccct cctccgaacg gtcgaagcgg
     1861 agcgagccgc cggccgcgct ggccacggcg tctagcagct gatccagcat gaacggcttg
     1921 ggaacgatgc ccgcgaactc cgcgcggagc gctcgatccg acgagtgctc ctgcgcctcg
     1981 agcacgtcct ccgcgtgggc ggtgaacatc acggtcggca cgggccgcgc acgcctggag
     2041 aggtacgccg cctcggccca gctcccgccg aactcggggc cctccgcgga gtccaggagg
     2101 atgcagtccg gctcgatccg gccgaccgct gccgcgatgg aaccgtgatc ggcctgggcc
     2161 agcgtcgcca cctcgtagcc ctcatcggtg aggatggcgc tgacgagctc cgcgacatgc
     2221 gggtcgttgt cgacgaccag cacgcggagg ggttcatggg tgggctcgga cgcccccgtg
     2281 ggctcaacca tgcgttgtct tctccggctc gggtgcacga cctgtcggtc tcgaaaaaac
     2341 gatcgcgaga aatgcaggca ccatgccagt cgtcggggtg tggcggctcg gttgccgccc
     2401 accggaacta acggccaaga actgtgcgcc gatcgctgct gcggtgccgc tggttgccgg
     2461 agagcgcaac ccccggccga tctgcgtcgc gtaccggccg ttgcgtgtcc gttagctccg
     2521 ctgaggggca accgccgtcc agcgacggtg gcgtgggcgg gaatatcggt cacttcatcg
     2581 ccgctcctcc cgccgcgtgg caaccgacca agcgacgcaa tggacggaag gcccacccag
     2641 gtcctattga gtcccaggtc tagggtgggc gtgttgccca tccggctcgc cgagtcctca
     2701 cgggaggagg cgatgcctat ggtcctcgtg cttcgcgatc cgagttagcg aagtggggcc
     2761 gcgacgccag gaagcgcgcg gtacccggag gaaccgagcg acatggcgaa gaacgacaag
     2821 ggtggcgaag gccgggccga tgcggcccag cgccgcaagg tgacccacgg ctacgaccgc
     2881 gggccgaagc agaagagcca gaagcgaccg cgtcgcaccc tcgaggagaa gctgcgccgg
     2941 tacgagtctc acgcggaggg ctacgagttc attccggcgc tgatgcactc gctccagcgc
     3001 gttgtcgagg ggccgccgaa gggcaccgag accgcggagg atcggcgtct ggcgcaggcg
     3061 ctgaaggagg ccggcgcgac ggagctgatc gcgcgagcgg ttcgcgcaca caaccagatc
     3121 ccgatggagc tgaagcggca ggccttctcc ccgcgcctgc tgacccggcc gctcgacgag
     3181 ccggtcgggg cggaggagac cgccgccatc gtccagcggg cggcgacgat ggtgaacgtc
     3241 agcgtcgcgg ccatcacgcc gagccgtctc gcgccggcgg ccgccgaccc caagcaccgc
     3301 ggcatgtgct gctgtcccga caccaccacc ccgccacgcg accctgagcc gcccaagccg
     3361 ccgccgaacc agtacgagct gacgttccag aagctctact gcgtcgacga gagcgatccc
     3421 gagtggtggg ggagcgacga gccgtacgtc gtgttcggca tcatcaccga ggagatggcc
     3481 gagtccggca ccgcggccca ggccgtggcg acgcccgtct acgaggacgt cgacgacggc
     3541 gacacccgcc cctcctccgg ggaccaggcg ctgcgcctgt tcggcttcac cggaccgcgg
     3601 gccatcgcga gctccgtgct gatcagcgcg acctgcttcg agcacgacct gggcgacgtc
     3661 tccgccacga ccgatgcggt ccgcgccgcc ctgactgccg tggccaccaa ggccgccgcg
     3721 gccgggggct acgtcgggtg gatcgtggcc ggggtggccg tcgtcgggat cggggtgagc
     3781 tacctggtgg acctcatcgg cgccgacgac aagatcgacg gcagccaggt catgagcctg
     3841 acccaggccg atgcggacgc ccgtaccgcc tccgtcaacc cgagcgtctt cccgccgctg
     3901 cacttcgatg gcggcgacgg cgacggcatc tacgacgtgt acctgaagct gcgccgcgtg
     3961 tagcggcgct ggtccacgcc cgggttgccg tggagcggaa ctaacggcga cgaagcgggg
     4021 cagcggggcc cttgctcagc cgctggttgc ccgccggtgc aactggcggc cgcgcagcgg
     4081 cccgtatcgg cggctgcctg tccgttagtt ccgccccggc gcaaccggca gggctgcctc
     4141 cgtaccgcgg gagcctcccc atccgtcccg ccgggcggca cgtacgggca cgctggggcg
     4201 cgaatcggcc ggagctttcc cgttagtgcc cgggcgagaa gaacgagcac ttcctcgccg
     4261 atacgcccgc cgggcggcaa gcggcgcgcg cagaatgcgg ccctacggag ggaggagcca
     4321 cccatgcggc cactgccaca cgtcccccgc gtcaccctgg ccatcgccgc gctcgcgttc
     4381 gccgccgggt acgccgtcgc cgcctggcgg tccagccccc agatggcgac cgggacggcc
     4441 tacgccgccg aggaccagat ctcggtcgag gccgacgact tcacctacgc gatccccgtc
     4501 gacgtcatct ggaccgatgc gaacggcacc gtccaccacg gcgaccgccc gggatgcctc
     4561 cccccgagcg gagatccgat cacggacgtc cgcttcgcct acgtcgaggc ctcgatcgag
     4621 ggcgcatcgt ggcggccggt ggtgtgggtc gactgccggt gacgccgcaa tcgtgaccga
     4681 cgagcatctg cgacggatcg ggcagcacct tccgacgctc gagcggccgg ggttccggtt
     4741 cggcgagtgg gtgccgtcac ggcgcgacga cggcggcgcg ctgcacctcg gctggtacga
     4801 gccgagcgag gcggcggagt ccttcctggc cgatgtccgc gcctggatcg agcccttcgc
     4861 ctggcccgcc tgggccgagt cggcggaggg tcaacggctg ctgggccacc cggacgccgt
     4921 cgcatcggct tcggcggagg acctgcggcg gctgctgatc gtctacgtgc gggccgagcg
     4981 attcggcgac gggacgctcg aggcggcgtt cgagtccggg atgctgaccg ccatcgttcg
     5041 gcgggcagcg gcactggccg gcgaagcgga cgcaccgggg cccgaaacgg ccggccgctg
     5101 aggcgcggcc ggccgctcga acggactcct acgagctgat ggtgccgtag agcttggtgt
     5161 cctcgaagaa gaggatcccg ttggcctcct gcaggccgat cagctcgccc tgcgagatgt
     5221 cccacgtcgg gccgctggtc gggtagttct cgacccggat gcgggtgtcg ccgaaggtcg
     5281 gcgccgtggc gtcggtcagg ctgtcggcga ggtagacgtt gccgtcggcg tcgaggaaca
     5341 gcgttccccg caccagcacc ggctgggaga ggtcaccggc gagcgcctcg gccagcgggg
     5401 tgcccgggcc gtcgatgacc gcgccgtccg cgacggtcag ggtcccgaca ggtgccggcg
     5461 atccggtcgg ctcgtcggtc ggatcgtcgc cgtcgctggc ggacggtgcc gtcggcgtcg
     5521 gggtggccgc gggcgacgtc gggctggagg gcccggcggc acacgccgcg aggaggaggg
     5581 aaatcgtcgc cagcgtggcg agcagcttag cggtggtctt catggtgtcc tgcgaacccc
     5641 caaggggcgg cgcctgggtg ggccgcttca gcgccgatcc tcgcaggtcg tcgagggacg
     5701 gccatctggc atcacgccgg tatggcctac gccatgtgtc cagtcacgca ggacggttca
     5761 ctgtccccgg ccacgcgtgg tgcgaaacct cagccgtgcg gcgacgtcgc gacgtctgcg
     5821 atgcgacggc tgcgagtgcc gatcccgctc gacatgcgga atccgcaggg gcacgggcgc
     5881 gccgcccagc aagagtgccc cgacgttgag aaggacgccc ggaacgtacc gccgaagctg
     5941 tcgccacatc gtcatgacgt actctcagct cgccggacgg tggcggggaa gaggcaatcg
     6001 gcacgtcggg ccgcgatgag gatgctgcgc cacgccagtc accactggca ccagatgcac
     6061 gaggccccca gccatgccgt acgagcccga tccgcgcgtc gacgcgtaca tcgacgcact
     6121 gcccgagtgg cagcaacgca tctgtcggga ggtccgcgag atcgcgcacg aggccgatcc
     6181 cgagctcacc gagacgatca agcgcaccgt ccagccgtac ttcgtgctgg acggcaacgt
     6241 ctgtgcgctc caggcgacga aggaccacgt caacgtgttc ctgtacgacg gcgggatcgt
     6301 gccggacccc gaggggatcg tgacccacgg ccacggcaat gagaccggcc ggcagatcgc
     6361 gatccacgag ggtgagccga tcaaccgatc ggcgctgctg gcctacctgc gccagatcat
     6421 cgccgacaac cgggcgggcg gctggcgtcg cctcaaggcc gcacggggtg agtctcgcta
     6481 gggcttcgcc ggcagcgcgc ccagctgctg catcatcccg aggcgatcgt actggtgcca
     6541 gtgctcggcg atccgaccgt cggccagccg gaaccagtgc atctcgccga tgcggtagcg
     6601 cttcccggcc gccggcaccc ccaggaagtc gccggtcggc gtcgcgctgg ccgtgagccg
     6661 caccgccacc cggtcgccct cggccagcac gtcctccacg tcgaaggcga tgtccgtcag
     6721 ggtgccgcgg atccgctccg tcgtggcgcg cagcttggcg cgcccgtcct cggtcatgcc
     6781 ccaggtgtgc gacacgaagt ccggcgcgac cagctgctcg accgattcgg gccggccctg
     6841 cacgaaaacc tcgtcgatga accggcgcac gagggccttg ttcgcatcgg cctggtcgcc
     6901 gttgggcgtg gcgcccttca tcggctcacc gcggaagtca ccctccgagc cgggatcgac
     6961 ctcgccctgg gacccgggcg tgtcgtcgtc caggctcgaa cgccgcgggt tctcagtcat
     7021 ctccatgtcg ctggttggtg cacggagagt gccacgctcc ggccgccctg cgccggcgga
     7081 atgcgcccgg ctcaggcctt ccagttgcgc tgacgcagct cgatcggtcc ggttcgcgcg
     7141 tatcggacgc tgggccgcag ccagcgcggg atgccagcct catctggtga tggaccgacg
     7201 cgctcgtcgg ggtcctcgag caggtacgga tcgagcggcg gcacgagctc gcggatcggt
     7261 ggcaggttga gcttgatcgg ggcatcggac gacgtcgctg gcgggatgtc ctggtcgagc
     7321 cgagccagcg ttgcgctcgc ggccgactcg gtgaccgccg cgggctgcgg ctcgggatcc
     7381 gtcggtcgtc gcctgcggat gagcaggaag aacagcgtca gacctcccgc cgtcgtcgcg
     7441 acgaacgttc ccgcggtcag cggcagctgc tcggcgagga aggtccccag cggctcgggg
     7501 tcgcgcggct gcggcgcggt cgagctcgct ggcgccgcgg ccaccggacc cccgacccag
     7561 gagtcatgca tcggtgccag cagtgcggcg gacttcgttg gcgccgggct gggggaggcg
     7621 gctgcggggg gctggaccac cgatggggcg acgggagcgg cgctgggcgg cgtcgtcggc
     7681 ggaggtgggg gcggcgcggt cggtgctccg ggagcgggag cgacggccgc cggtgcggcc
     7741 acgggcggcg gaggcggcgg aggcggcacc gcggcgactg gcgtggcgac gggagccgga
     7801 acgggggtgg ccgtgggcgc aggcggagcg acgacgacgt tggtcggcga cacgccggtc
     7861 agcgtcaccg tggtggtccc tcggccgttg ccactggtga cggtgaagga gtacgcgcgg
     7921 gtcccgaccg gcaggttcag cgacgcggag taggtgacgc ccgaggcgta atcggtcccg
     7981 gagccggcga gcgggaaggt gccgaccccg ctgatgctga cgacgacctt cgatggcgcg
     8041 caggcggcgg tatcggtgta ggtgacggag aaggtgacgg cctcgcccgt cgtgacggtc
     8101 gatggggtga cggtgccggc ctggaggctc gactcatgcg aggcgccatt gcagctggca
     8161 gccgccaccg gcgtcgctgc gggcagcagc gcgacgagga acgcgacgag cgacgccccc
     8221 agtctcagct tcttca
//
LOCUS       DASTYP010000036         9488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_220578, whole genome shotgun sequence.
ACCESSION   DASTYP010000036 DASTYP010000000
VERSION     DASTYP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220578"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..819
                     /locus_tag="VFV59_01640"
     CDS             301..819
                     /locus_tag="VFV59_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5324559.1"
                     /translation="MAQSPSEYPRTSDRGNESVIDPHARFNGKYVSDRDLRIEGEAQG
                     EIECQGTLIISPQARVRSAIKAHNVIVNGDYEGDVDCGGRFEIGSTGRVKGNIKTQVL
                     VVKEGAHWEGGVTMTREPGPRPSSPANQGGGNAQSRPASGGGSSIFSKQDREGAPANQ
                     PEREGQPAGQGQ"
     gene            886..1971
                     /locus_tag="VFV59_01645"
     CDS             886..1971
                     /locus_tag="VFV59_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU5324560.1"
                     /translation="MTPPRARRRAQRPATPVLVEVLRGDIVESVHRGDIVVATPGGEV
                     VASVGDPDRLIFWRSSAKPFQLAPFVASGRFDEYQLGSEALAIMAASHSGEDRHVRLV
                     QEILRRAGLSSAVLHNQVHAPYDTETAQRLLRDGEKATVLRGNCSGKHAGMVLFAKAA
                     GWPIEEYWRPQHPVQRQALEVVSAVSDVPIEEIVTAVDGCGVVTFGTPLRGLAIAFAR
                     LADPGGVADPPLRSALLRIRDAMMSHPELVAGERRRIDTALMRAYPKRIVSKGGAEGV
                     LAMGLPPGAVGSRAPFGAAPLGVAAKVEDGNLARRGGDVTSVEVLRQLGLVSDPVAEA
                     LDAFVHPPIVDPRGEETGSVRPAFRLA"
     gene            complement(1981..2715)
                     /locus_tag="VFV59_01650"
     CDS             complement(1981..2715)
                     /locus_tag="VFV59_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEU5324561.1"
                     /translation="MFLTGTLLNVATVLAGTVLGLLLGGRMPRRMQESLTIGLGLFTA
                     LIAMSMGLRIFAEPAQAGDDLAVLAAVLLGVAIGEALRLHDGLEWLGGWFQRRLSSGE
                     RPSRIAEGFVTASLVFCVGPLTILGSLDNGLTGDVRLLAIKSLLDGVAATAFAAALGP
                     GVALSAITVFVVQGGIAAGAFLLRDVMDTATILAITAAGGVILLAVALRLLDLRAVRV
                     ANFLPALLLAPIFIRIADAVRQALSA"
     gene            2776..3363
                     /locus_tag="VFV59_01655"
     CDS             2776..3363
                     /locus_tag="VFV59_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU5324562.1"
                     /translation="MRYVALGDSYTIGTSVRAKDRWPDQLVRRLPSLELIANPAVNGF
                     TSADLIEHELPGLAAWRPELVTVLIGVNDVVRGVDEDEYARNVDVILDRLTERVGASR
                     VVAVAIPDYTVTPRGGDYGDPTERRAAIGSFNAIMAAGCGRRAVAFVADTWEISRRAG
                     EDPSLVAEDGLHPSGAQYRLWVDAIAPVVARLLGD"
     gene            complement(5462..6844)
                     /locus_tag="VFV59_01660"
                     /pseudo
     CDS             complement(5462..6844)
                     /locus_tag="VFV59_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            complement(6875..7357)
                     /locus_tag="VFV59_01665"
     CDS             complement(6875..7357)
                     /locus_tag="VFV59_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324563.1"
                     /translation="MDFRPDDLYDQYPSGEELGYGRDQGYNGFVKLNDRALFRPELLD
                     PDSPDYLPDLAAFVRLGEEPNDDDPFPPISVNFAQFKSSTREWEWALHMPHLAMAGRV
                     EGIDSGIDNFPEPEEARIGTYFINHDRTLAIRKLSTIVMEDGAQCGQMIYKHWLPETE
                     "
     gene            7465..8961
                     /gene="gltX"
                     /locus_tag="VFV59_01670"
     CDS             7465..8961
                     /gene="gltX"
                     /locus_tag="VFV59_01670"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5324564.1"
                     /translation="MSASDDQRPMRVRMAPSPTGPLHIGTARTSLYNFLTARHAGGTY
                     VLRIEDTDTARSTVDFERDIIDNLHWLGISWDEGPQVAGGDDVGPHAPYRQSERMELY
                     AIEAKRLLEAGRAYRCWCTPDELEAVRREQQARREAPRYNGRCLSLTDAERAAFEAEG
                     RTSVIRFRVEPEKVRFDDLIRGEVEFDNALLGDFVIVRNDGVPLYHFVVVVDDQTMEI
                     SHVIRGEDHLSNTPKHIALIRALGYREPRFGHIPLILNPDRSKMSKRKSQTAMTAYRE
                     LGYLPEAMVNFLAFLGWSPGTEEEIFSLDELVQRFEIDKVHKGGAVFDRDRLDHLNGV
                     YIRSLTDEQLALRLRPWVPEAVADADLLRLVPLVKERLVTLGDVVALVGFAWEPDEVV
                     ASWYDRELLYPKKGGPSDARRALEGARTALEDLDDADFSADVLEQRCRQAAEAAGMKA
                     GEFFSPLRVAVTGRTVSPPLFASLELLGRDRSLARVDDALGKLAEVPA"
     gene            8961..>9488
                     /locus_tag="VFV59_01675"
     CDS             8961..>9488
                     /locus_tag="VFV59_01675"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEU5324565.1"
                     /translation="MDRDALAGARAPRLDELIDRELLVPIRSSSGELPDATQLGMEAF
                     RHGTDESGERFLAAFTAPDVFAEFGPPGSDHVRMAARDLFERADAAQERVVIDPGAPT
                     QVEVSTGVLPFLAAGIDPNRPDALRARRPLGELPPLEVPEQVPEPFGRELRTALEELP
                     HVERAWLLRAGTAWTA"
BASE COUNT         1344 a         3407 c         3464 g         1273 t
ORIGIN      
        1 ccttcggcgc gcgcgtcggc gaggcgatcg aggcggcgcg gcgcgcatcg gtccagtcgc
       61 aggggtagag gttggtacgc cacgtgccgt ccaccgcacg gcactatctg ccccggcggc
      121 atgctgctac gctatcgctc cttgcactcg cgcacggacc gtgcccagcc cgtcgccgga
      181 tccgcagctg aggagaagag catggtcttc ggatcgaagc gtgaaaccaa gccggacagt
      241 ctctcgcgcg aggacgttgt cgggaacctg tatccggata gcctgacgag tgaggtcgac
      301 gtggcacagt cacccagcga gtacccccgc acctctgacc gcggcaacga gtcggtcatc
      361 gatccccacg cccgcttcaa cgggaagtac gtcagcgacc gcgatctccg catcgagggc
      421 gaggcccagg gcgagatcga gtgccagggc acgctgatca tcagcccgca ggcgcgcgtg
      481 cgcagcgcca tcaaggcgca caacgtgatc gtcaatggcg attacgaggg cgacgtggac
      541 tgcggcggcc gcttcgagat cggctcgacc ggccgcgtga agggcaacat caagacccag
      601 gtcctggtgg tcaaggaagg cgcgcactgg gagggcggcg tcaccatgac ccgcgagccc
      661 ggcccccgcc cgtcctcgcc ggcgaaccag ggcggtggca atgcgcagtc gcgaccggcc
      721 tccggtggcg gcagcagcat cttcagcaag caggaccgcg agggcgctcc cgccaaccag
      781 ccggagcgcg agggccagcc ggccggccag ggccagtagc cccactccga tccgaatgac
      841 cgaccgggcg ctgcacgacg gcgcccggtc gctctgtgtc cgccgatgac gcctccacgc
      901 gctcgtcgcc gcgcccaacg ccccgccacg ccggtcctgg tcgaggtcct gcgcggcgac
      961 atcgtcgagt cggtgcatcg gggggacatc gtggtggcca cgcccggcgg cgaggtcgtg
     1021 gcgtcggtcg gtgatccgga tcggctcatc ttctggcgct ccagcgccaa gccgttccag
     1081 ctggcgccct tcgtggcctc cgggcgcttc gacgagtacc agctcggttc ggaggccctc
     1141 gccatcatgg cggcgtcgca ctccggcgag gatcgtcacg tccgcctggt gcaggagatc
     1201 ctgcgtcgcg ccgggctgag cagcgccgtg ctgcacaacc aggttcacgc gccgtacgac
     1261 accgagaccg ctcagcggct gctccgcgac ggcgagaagg cgaccgtgct gcgcggcaac
     1321 tgctccggca agcacgccgg gatggtgctg ttcgccaagg cggcgggatg gccgatcgag
     1381 gagtactgga ggccccagca tcccgtgcag cgccaggcgt tggaggtcgt atcggccgtg
     1441 tccgacgtgc cgatcgagga gatcgtgacc gcggtcgacg gctgcggggt cgtcacgttc
     1501 gggacgccgt tacgcggtct ggcgattgcc ttcgcgcgtc tcgccgatcc gggtggcgtg
     1561 gcggatccgc cgctgcgctc ggccctgctc cgcatccgcg acgcgatgat gtcgcacccc
     1621 gagctggtgg ccggggagcg tcgtcggatc gatacggcac tgatgcgtgc ctatcccaag
     1681 cggatcgtct ccaagggcgg tgccgagggc gtgctggcca tgggtctgcc gccgggcgcc
     1741 gtcgggtcgc gggcgccgtt cggggcggcg cccttgggcg tggccgccaa ggtcgaggat
     1801 gggaacctgg cgcgccgcgg cggtgacgtc acctccgtcg aggtgctgcg gcagctcggc
     1861 ctggttagcg atccggttgc cgaggcgctg gacgccttcg tccatccgcc gatcgtcgac
     1921 ccgcgcggcg aggagaccgg gtcggttcgg ccagccttcc ggctcgcctg agcaggccga
     1981 tcacgccgag agcgcctggc gcaccgcatc ggcgatgcgg atgaagatgg gcgccagcag
     2041 cagcgccggc aggaagttcg cgacgcgcac cgcgcgcagg tccagcaggc gcagcgccac
     2101 cgcgagcagg atgacgccgc cggccgcggt gatcgccagg atggtggcgg tgtccatcac
     2161 gtctcgcagc aggaacgcgc cggccgcgat cccgccctgc acgacgaaga cggtgatggc
     2221 cgacagcgcg acgcccgggc cgagcgccgc ggcgaaggcg gtggccgcca cgccgtccag
     2281 cagcgacttg atggccagca gccggacgtc gccggtgagc ccgttgtcga gcgagccgag
     2341 gatcgtcagc ggcccgacgc agaagaccag gctggcggtg acgaatccct ccgcgatgcg
     2401 gctcggccgc tcgccgctcg agagccgccg ctggaaccac ccgccgagcc actccaggcc
     2461 gtcatgcagg cgcagcgcct cgccgatcgc cacaccgagc agcaccgcgg ccagcaccgc
     2521 caggtcgtcg ccggcctgcg ccggctcggc gaagatgcgc agccccatgc tcatggcgat
     2581 cagcgcggtg aagagaccga ggccaatggt gaggctctcc tgcatgcgtc gcggcatgcg
     2641 gccgcccagc agcagcccga ggacggtgcc ggccagcacg gtcgccacat tgagcagggt
     2701 cccggtcagg aacacggtcg agccacgcgc atgggcggta gggtagcggc gccggagagg
     2761 aggacagccg aaccgatgcg ctacgtggcg ctgggcgact cgtacacgat tgggacgtcg
     2821 gtgcgtgcga aggaccggtg gccggaccag ctggtccgcc ggctgccgtc actcgagctg
     2881 atcgcgaacc cggcggtcaa cggcttcacg tcggccgacc tgatcgagca cgagctcccc
     2941 gggctggccg cgtggcggcc ggagctcgtg acggtgctga tcggcgtcaa cgacgtggtg
     3001 cggggcgttg acgaggacga gtacgcgcgc aacgtcgacg tcatcctcga ccgattgacc
     3061 gagcgcgtgg gcgcctcccg cgttgtcgcc gtggcgatcc ccgactacac ggtcacgccg
     3121 cgcggcgggg actacgggga tccgacggag cgccgggcgg ccatcggcag cttcaacgcg
     3181 atcatggcgg ctgggtgcgg ccggcgggcg gtcgcgttcg tcgccgacac gtgggagatc
     3241 agccggcgcg cgggggagga cccgtcgctg gtcgccgagg acgggctgca cccgagcggt
     3301 gcgcagtacc ggctgtgggt cgacgcgatc gcgccggtcg ttgcccgtct gctgggcgac
     3361 tgaccggcca gtcgcccggc aggaggcggc gcagcccgac cgcgccgagc gagccgagca
     3421 attcggacag ccggtcgggt ggctcgtcgc cgagggcgag gtgcgcctgg tccagcaggc
     3481 atagagcgcc cggcagcggc aggtcgaggc cgtcccagag ctcgcgggcg cgcgcgtaca
     3541 ttcgagccgc atctccggag cgcgtgagcg cggccaggcc cgcctcgagc gtcacgaccg
     3601 tcgcctgggc ggcgcggccc cacctgatct cctcgcgcaa gcgatcgatg tcggtggcgg
     3661 ccgtggcggc gtcgcccatc cacagcgcga tgcgcgtggc gatcgcccgt tcgtgggggt
     3721 ggatggcgcc ggctaccgcg gcgaggccct cgcgcgccag gtcgcgcgcc tcgtcgagcc
     3781 gaccatccag gaaggcgcac cacgattgcg ccacacgaac ggacccgacg acctgcgggt
     3841 ccgtcgtcga cggcagcgga tcgaaccggt cgagggcggc gaggggcgcc tcggagccct
     3901 cgagggcgtg gatcgtcgcg gccgtggccg acagcacgat ccgatacgcc tcggggacgc
     3961 cacgctcctc gagcgccgcg atggtctcga gcgcccaatc ccacgccccg gtctcgatcg
     4021 cggcggcgca cgccaggtcg gccagtggca ccgcgacgtc gccgacgccc atctccgtcg
     4081 ccagtgccag gccggcctgc gcgatctcga gggcggcgcg gggatcgtcg gccagcatgc
     4141 cccaggcgag gttgttgcgc gcgcgcagct ccgttcgcac catcccggcc tgctccgccc
     4201 tcccgatcgc gtcccggagg tcggcgaatc ccggctcatg ctcacccagc ccgatgcgcg
     4261 cggtcccccg cgtgatcaac aggtcggtga ccacggccgg caggtcgagt cgctcggccg
     4321 cttcgagggc gatgccgctc caccgcaggg cttcgcgatc gtctccgagc agcgatcgag
     4381 cccgcgcgag ctgggccgcg agctccaccc ccgacggatc gctgcgccag cggcgcatgt
     4441 cgcgcatggc cgcctccagc tcgaggagcg cgggctcgtt cgcctgtccg gtgagcatca
     4501 cgctcgccag ctgcgcgcgc gcccgcgccg ccgagctgcg gtcaccggcg cgccgtcgaa
     4561 gctcgatcag ctcgcgcagg cgggcctccg ccacctcgat ccggccggcc gcgcggccgg
     4621 catccgccgc ctcggccagc agggccggcc gctccacggc cgtcgcgagg ccgagcgcgg
     4681 tgtcgatcag gtccagggag cgcgccggga cgtggagcgc gctcgccgcc cgcgatgcgc
     4741 ggcggagggc gctgaccgcc cggtgggcga tccgttccgc ttcgcgcgga tcggtcgtca
     4801 atcgatgcgc cgcgaccagg tgcgccgcca actgctcggg tggctcgtcg tccagccgat
     4861 cgaggtagcg ggcggccgcc aggtggagcg tgcgacgctc gaggcgggag agggtctggt
     4921 aggcgacctc ccgcacgagg tcctggacga acgtgagctg gccgcggccg ggggagccca
     4981 gctcctcgtc gagggtcagc atctcccgcc gcacgaggcc atcgaggagg tcgcgcaggt
     5041 ctgccgcatc ggcgtcgacc acggcgtgca cggcctccga ccggaagcga ttgccgagga
     5101 cggcggctgc catcagcagg cgccgctcgg caaggggcag ggcatccagc cgggcggcga
     5161 tgatgccgtg cagcgagtcc ggtaccggcc ccccggcctc ctgcgcctgg ccgccggcgc
     5221 cccggcggtc gacgagcatg cgcgcgacct cgacggcgta gaggggaacg ccgccagcgt
     5281 cgcggaggag cggctcgacc agcgtgggag gaagatccgg cgctcggtcc gcgagcagct
     5341 cgcgcatggc ggcctcgtcc aggcgatcca gggtcatggc ggtgaagctg ccgacggccg
     5401 atccccacgt cgggcgcgcg tcgaggagct cggggcgggc gagggtcagg atgaagatcg
     5461 gtctcgttcg tgaccaggtt gccaggtgct cgatgaagtc gaggaggctc ggatcggccc
     5521 agtggaggtc ctcgaacacc atcaccaccg ggctgagctc ggacacgcgc tcgaagaacc
     5581 ggcgccacgc cgcgaacagc tcatcgcggt cgaagtctcc ggccgtctcg ccgctgagca
     5641 gcgccgccag gcggggactc atccagcggc gctcgccctc gtcgcgcacg agtccgtcca
     5701 gcgccgcgcc cagctgtcgc agggccagct ggggaccggc gtcatcggcg atgcccgcac
     5761 gcctccgcac catccgcgcc accgccgaga aggcgacgcc ctcgccgtag gccagggctc
     5821 ggccggcgtg ccaggcgatg acgcgcggat gggcatcgac ccagccctcg agctcccacg
     5881 ccaggcggct cttgccgatc cccgcgatcc cggtgaccga gaccagccgt ccccgtccct
     5941 cggcgatgac cgactccagc agctcggtga tctcccgcag ctcgcggtcg cggccgatga
     6001 ggcgtccggc atgggacgcg ctgcgcaccg tcgcgtcgtc ccgtcggctc gtcacgcgga
     6061 aggtctcgag ccgccccttc cggcccttca ggctcagcga tcccacgcgg gcgaacgcga
     6121 cgaccttcga cgccgcgtcg cgggtcgccc ggtccaccac gacgccgccg gcgggcgctc
     6181 ggccctggag ccgggccgcg acattgacca ggtcccccgc cacgatggcc tggtcgacca
     6241 tgccgaccga gacggccgcc tccccggtcg tgacggcggc gcgcgcctgg agcacccggc
     6301 ccgaggctgc tccccccatg cgctcgaccg cgtagacgat cgcgagggcg gcacggacgg
     6361 cgcgctcggc gtcatcctcg tgtgccgatg gagcgcccca cacggccatc acggcgtcgc
     6421 cgatgaactt ctcgatggtc ccaccgtggc tggccacgat cgaccgggcg acctcgaagt
     6481 agcgtgcctg gacggcacgc acctcctcgg gatccatgtg ctccgccagc gccgtgaagt
     6541 cgacgaggtc gacgaacagg atcgagacgc gccgtcgctc cgccacgccg atgcgaccgg
     6601 aggcggcggg ttgggtggcg ccatccgtcc cgcccgccac cggcgtctcg cccgggctcg
     6661 acgtcgcggt cccgcactgc gagcagaagc ggctgcccgg ggcgacgggc gtcccgcatg
     6721 ccggacagga gcccatcggc tcgagccgca cgccacaggc cggacagaat cgggcgccgc
     6781 gtggcacctc cgccgcgcag gacgggcagg tggccgaggg cctaggcgag tctccggccg
     6841 gcatgcatcg cgactcgagc gaaccccgcg cgctctactc ggtctcgggc agccagtgct
     6901 tgtagatcat ctggccgcac tgcgcgccat cctccatgac gatcgtcgag agcttgcgga
     6961 tggccagcgt gcggtcgtgg ttgatgaagt acgtgccgat ccgggcctcc tcgggctccg
     7021 ggaagttgtc gatcccgctg tcgatgccct cgaccctgcc cgccatggcg aggtgcggca
     7081 tatgcagcgc ccactcccac tcgcgcgtgc tgctcttgaa ctgcgcgaag ttgacgctga
     7141 tcggcgggaa cgggtcgtcg tcgttcggct cctcgcccag tcgcacgaag gcggccaggt
     7201 ccggcaggta gtccgggctg tccgggtcga gcagctcggg gcggaacagg gctcgatcgt
     7261 tgagcttgac gaagccgttg tagccctggt cacggccgta gccgagctcc tccccactcg
     7321 ggtactggtc gtagagatcg tcgggacgga agtccatgct cgggtcctcc tcgtgcgcct
     7381 gcggcgatca taggtccgcc ccagtcgcct gcctaccccg agggaagggc tgctccggcg
     7441 gccgcctgct agcatcggag cccaatgagc gcctccgacg accagcgacc gatgcgcgtg
     7501 cgcatggcgc cgagccccac cggcccgctg cacatcggca ccgcgcgcac gagcctctac
     7561 aacttcctga ccgcgcgaca cgccggcggg acctacgtgc tgcgcatcga ggacaccgat
     7621 accgcccgca gcaccgtcga cttcgagcgc gacatcatcg acaacctgca ctggctgggg
     7681 atcagctggg acgagggtcc gcaggtggcg ggcggtgacg acgtcgggcc gcatgcgccg
     7741 tatcggcaga gcgagcggat ggagctgtac gcgatcgagg ccaagcggct gctggaggcc
     7801 gggcgcgcgt atcggtgctg gtgcacgccg gacgagctgg aggcggtccg acgtgagcag
     7861 caggcccgca gggaggcccc gcgctacaac gggcgctgcc tgagcctgac cgatgcggag
     7921 cgggccgcct tcgaggccga gggtcgcacc tccgtgatcc gcttcagggt cgagcccgag
     7981 aaggtgcgct tcgacgacct gatccgcggc gaggtggagt tcgacaacgc gctgctgggc
     8041 gacttcgtga tcgtccgcaa cgacggggtg ccgctgtacc acttcgtggt ggtggtcgac
     8101 gaccagacca tggagatcag ccacgtcatc cgcggcgagg atcacctgtc gaacacgccc
     8161 aagcacatcg ccctgatccg ggcgctgggc taccgcgagc cgcgcttcgg gcacatcccg
     8221 ctcatcctca acccggatcg gtcgaagatg agcaagcgca agtcgcagac ggccatgact
     8281 gcctaccgcg agctgggcta cctgccggag gcgatggtca acttcctcgc cttcctgggc
     8341 tggtcgccgg ggaccgagga ggagatcttc agcctggacg agctggtgca gcgcttcgag
     8401 atcgacaagg tccacaaggg cggcgccgtg ttcgaccggg atcggctcga ccacctgaac
     8461 ggcgtctaca tccgctccct gaccgatgag cagctggcgc tgcgcctgcg cccctgggtc
     8521 cccgaagcgg tggctgatgc ggacctgctg cgcctcgttc cgctcgtcaa ggagcggctc
     8581 gtgacgctcg gcgacgtcgt cgcgctggtg ggcttcgcgt gggagccgga cgaggtggtc
     8641 gcgagctggt acgaccgcga gctgctctac ccgaagaagg gcggcccgtc cgacgcgcgg
     8701 cgtgcgctgg agggcgcacg caccgccctg gaggatctcg acgacgccga cttctcggcc
     8761 gacgtcctcg agcagcgttg ccgccaggcg gccgaggcgg ccggcatgaa ggccggcgag
     8821 ttcttctcgc cgctccgggt ggcggtgacc gggcggacgg tgtcgccgcc gctgttcgcc
     8881 tcgctcgagc tgctggggcg tgaccgctcg ctggcgcgcg tggacgacgc cctgggcaag
     8941 ctggccgagg tccccgcctg atggaccgtg atgccctggc cggcgcccgc gcgccgcgcc
     9001 tggacgaact gatcgaccgg gagctgctgg tgccgattcg ttcgtcttcg ggagagctcc
     9061 ccgacgccac gcagctcggg atggaggcgt tccgccacgg caccgacgag tccggcgagc
     9121 gcttcctggc ggccttcacc gccccggacg tcttcgccga gttcgggcca ccgggcagcg
     9181 accacgtgcg gatggcggcc cgcgacctgt tcgagcgcgc cgacgccgcc caggagcgcg
     9241 tggtgatcga tcctggggca ccgacgcagg tcgaggtgag caccggggtc ctgccgttcc
     9301 ttgccgccgg gatcgatccg aaccgaccgg acgcgctgcg cgcccgacgg ccgctgggtg
     9361 agctcccgcc cctcgaggtg cccgagcagg tcccggagcc gttcggccgc gagctgcgca
     9421 ccgcgctcga ggagctgccg cacgtcgagc gggcgtggct gctgcgcgcc gggaccgcgt
     9481 ggacggcg
//
LOCUS       DASTYP010000037        32872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_183883, whole genome shotgun sequence.
ACCESSION   DASTYP010000037 DASTYP010000000
VERSION     DASTYP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 32872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32872
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183883"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFV59_01680"
     CDS             <1..525
                     /locus_tag="VFV59_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324566.1"
                     /translation="GIALSAAAVTWSVGAWIQARAAGAGTRGTLVAAGATLIAVGIGL
                     AALVLLPELPVLAATAGWAVGGLGMGLAYSMLAFLTLETAPPGEEGASSASLQLMFTL
                     GTAFGAGAGGAVVALADRGALELVEALGVVDAMMVVVAVTAMVVALRVPRRPAPRTDA
                     LVATPPPVPAAELG"
     gene            582..1559
                     /locus_tag="VFV59_01685"
     CDS             582..1559
                     /locus_tag="VFV59_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5324567.1"
                     /translation="MTPTRRILAIDGGGVRGVIPAALLASMERATGRATRDAFDFVAG
                     TSTGAVLTAGLVAGIPAQRLVSLYVDRAAEVFRSVPLLSFVQRLVAGHQYDTAGLHRV
                     IREELGAPAADWRLNDAPVDLLVTAKRLSDGMPWYFVRDDPANAQRAGAIRLADAVTA
                     SAAAPTFFAPWTIGGIGELVDGGVGVAGNPVYQACVEAFEYTDRYLPGETVVVSLGTG
                     RVLQRERPTWLGSWIGWLLSELLRSPAEQQTELVHRHYPAVALSRIDVELPGAIGMDA
                     ADRVDELRAVGERLATTVDWEALLDGRDERFRVTGERTLPREYVSRDPG"
     gene            complement(1620..2807)
                     /locus_tag="VFV59_01690"
     CDS             complement(1620..2807)
                     /locus_tag="VFV59_01690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5324568.1"
                     /translation="MSSSERADVVIVGGAIVGSAVATFLASRPDFGGRIVVVERDPTF
                     RTSSTTLSAASIRLQFSTPLNIDISRFGLDVVKHPDRYLAVDGEVPQLDFVENGYLFL
                     ATDAGLATLEHNHAVQRARDVPVTLLTPSELAARFPWMSVDDLAGASLGLADEGWFDA
                     YALLQGVRRKARSLGVAEIVGEVTALDLDGDRVAGVRLADGSAIAADWVVNAAGPRAA
                     DVAAMAGVELPVRPRKRLVYHFESPAMLGAAPLTIDPSGIYFRPEGPAYLAGFSPHGN
                     DPDPDTLDLSVDYAPFESFVWPTIAQRVPAFDRLRLLDGWAGHYEVNTLDHNAVVGPH
                     PRIANLLFANGFSGHGLQQAPAVGRGLAEWITLGRYESLDLSPLGYERIEREAPILEL
                     NVV"
     gene            complement(2825..3058)
                     /locus_tag="VFV59_01695"
     CDS             complement(2825..3058)
                     /locus_tag="VFV59_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324569.1"
                     /translation="MTDQNANVTSGETHGQDQEPREVGAADPAVRDPRPDGPMKQPVD
                     PEAASDEFGASPDAAYGQREGQADPETGSLPDR"
     gene            3175..4389
                     /locus_tag="VFV59_01700"
     CDS             3175..4389
                     /locus_tag="VFV59_01700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5324570.1"
                     /translation="MLSASWPSPVRLPRPVPPPHVRRRVRAAERRAIRRSVVMASPLV
                     AVFFVVDVLLRVSTHSTGALAFAPWELAGAAFALAMAVLARRSLVRSEAAALAVVAVT
                     VAIALLGIQLVPDGRMLATAQLAILVVGTGLFIPWSHWWHVSAMLVSVTMSAAFVVAP
                     WTGGLPPHRGMNLVVAVMSAAMISLIGHATWQRRLRALLVQQFALRHLSRYAQRQEAH
                     VTELNRELNLVARRDALTGVGNRLALDEALDRLLAREDRLRPMRFALALFDIDHFKAY
                     NDEHGHLAGDAALERLGAILRRATRNDDLAFRYGGEEFLVLLPDVSLTDAIAVAERIR
                     VAAEEDETRALPPFTVSGGVALCDPADGHDAEPLLRRADAALYLAKRAGRNRIAADEL
                     SVAMQRADLASA"
     gene            complement(4402..5031)
                     /locus_tag="VFV59_01705"
     CDS             complement(4402..5031)
                     /locus_tag="VFV59_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324571.1"
                     /translation="MAIAMAGVLAFAAVLALAITWWSRDAGVARDEVALPSIVVGTPA
                     GFVLNRASPTATAPQPTVTATPVPTPRDTPVETATPVAVALGPTPVPPPPRTPAPTSI
                     VTSAAEPVDAVTAFYGAVAAGDFDRAYALWSDRMKAEYPRQENLDERFAETASVEFTE
                     LFVASRTATTATVQANFVETYDSGATRQFVGYWQLVLVEGRWLLDHPTY"
     gene            5252..6139
                     /locus_tag="VFV59_01710"
     CDS             5252..6139
                     /locus_tag="VFV59_01710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5324572.1"
                     /translation="MTGVKFGLQINQFTWPGGVAGMAPTLARVTRTADDAGFDSIWVM
                     DHFFQIRGLGPPEAPMLEGPTALGFMAAHSERARLGLMVGGVHYRAPGLWIKATTTLD
                     VLSGGRAWFGIGAAWNEQESRGLGFRFPPLRERFEQLEDVLRMAHAAWSGGTGTGERF
                     EGTHVTAERILNSPQAVSRPRVPILVGGGGERKTLRLVAQYADACNIFGRSPAFVRDK
                     LAVLRGHCERLGRPYDEIEKTVLTSVDPAEEASAATVERFGGLAEAGAQHIIFSVRGV
                     GADPGRLERLASEVFPQLR"
     gene            complement(6151..8118)
                     /locus_tag="VFV59_01715"
     CDS             complement(6151..8118)
                     /locus_tag="VFV59_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5324573.1"
                     /translation="MELLPVGLLAEVFATLAPMTPEEARRRAETAVETLSATRDRSEV
                     AQLGLVLRALELPIVTALDGAGLTPFSRAPQAARERILRAWATSRIPQRRTAFQALKR
                     IGMFLAYADPGDDPAQPSNPMWERIGYVPHRAAVVDVDIPEVRPLEVPREGAEPLRLE
                     ADVVIVGSGAGGGVVAARLASAGRSVLVVEAGRHVPAAGVTSSEAIGWRDMYLDRGTT
                     GPDDASVTILAGATVGGGTTVNWTTSIPPPEWVVDEWEGEHGLGKLRGTTADADLARL
                     TDELGLMPPTEVPPKDQLILDGARALGWDADTTRRNAGPCTDCGACSFGCHRGAKRST
                     ARVHLVAAQGTGARLLAEAQVTRVLLGDGRAAGVRGRLAGGRPFVVHAPQVVVAGGAL
                     RTPILLQGSGFDHPQIGRNLHLHASPVIAAQLPHRVAMWEGPTQAARSAQFLGPDRSI
                     LIESAPAHPGLAAAALPWLGRDAGTELLSRLDRLAPLIGILRDRSAGIITRRRSGRAS
                     IGYRLATADRVAARAALVAMARMAEAGGADDMVALTTEGERWRRGEDFDAYLERLATV
                     DTGPNRLALFSAHQMGSARAGADPRQSACDPHGRLRADQRGALVPGVYVADASLFPTA
                     LGVNPMLTIMLLAERAARAVLADGPSPAPVR"
     gene            complement(8133..9830)
                     /locus_tag="VFV59_01720"
     CDS             complement(8133..9830)
                     /locus_tag="VFV59_01720"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5324574.1"
                     /translation="MRLSQLFFASLRDDPAEAEMVSHKLLVRAGYVRQLGAGIYSLLP
                     LGYRVQHAIEEVIRQEIDRIGGQEMEMPVVHPAELWKESGRYAKIGPEMVRFKDRSGR
                     DMVLAMTHEEVVADLTRDLVRSYRQLPAIVYHFQTKFRDEPRARGGLIRVREFVMKDS
                     YSIDADEAGLDRSYDRHYEAYTRIFQRLGLDAIAVSSDVGIMGGTGAHEFMVLNPHGE
                     DTLVLCDACGFAANQQIAPNAVVADTTEEPMPLEDVDTPGTETIAALADYLGIPTAKT
                     AKAAFFVTGDGRLVTAIVRGDFEVNETKLVNAVKAIGGTRPAQADEIRRAGMEPGYGS
                     PVGAHDTVVVVDRLVAESPNLVAGANRPGVHLRNVNVPRDYQPDVVDDISDVRAGDPC
                     PDCGQPVRLAQGIEVGNIFKLGSDFTVALGATYLAEDGSRRHPIMGSYGIGLGRAMAC
                     IVEAHHDEKGIVWPAEVAPYRAHLVAIGAAKDAAVGEAADALYERLTDAGVSVLYDDR
                     DESPGVKLTDAELLGMPTILTVSPRALAAGGVEVTDRASGERAVRPIDEVASELIGA"
     gene            complement(9843..11162)
                     /locus_tag="VFV59_01725"
     CDS             complement(9843..11162)
                     /locus_tag="VFV59_01725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324575.1"
                     /translation="MRRSIFAVLLGTFTLRFSTGLTGALLVFYLAELETFGAPKVSAI
                     EVGLLTAAFFLAELVLSPLFGAISDHLGSRRVMQYGPIFGAVAVVLTFLTTDLLLLGV
                     TRWLEGAAAAASIPSILGFIALATSRDEALRGRTVARFEAATLAGLGLGLVAAGGLWQ
                     VLDRNAFLLNAGLYGISFLIYRYGVTVDPGRASEGEAEEIEAGGLAATARGFSWSRYR
                     AVLVSRSVWLLAPTWIAVNAFIGAWTSQSIFQLVNEPRPGFEDQRLMGGYESWQVSIG
                     LAVALVVFFAGLWYWGNRFKAFRRTTIMAIGIVGGLAMVLAIFAINHAEAWPLPAQLV
                     AGVVMAGGLFVLAGATPAALGMLADISEEHPADRGAIMGLYSVFLAVGQVVGSLAGGG
                     AAEWLGIDGLLLASAGLLLVALGPLRWLRDSEHLVGGPSAEPVPATR"
     gene            complement(11253..11588)
                     /gene="trxA"
                     /locus_tag="VFV59_01730"
     CDS             complement(11253..11588)
                     /gene="trxA"
                     /locus_tag="VFV59_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5324576.1"
                     /translation="MSNVQEVTDATFDTDVLQSEKPVIVDFWAEWCGPCRMVAPEIDK
                     LASKYAGSVEVRKLDVDANPQTAMKYGIMSIPTVAIFAPGEAPRAAVGFRPAEALEEA
                     FGLSRFSNS"
     gene            complement(11625..11927)
                     /locus_tag="VFV59_01735"
     CDS             complement(11625..11927)
                     /locus_tag="VFV59_01735"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5324577.1"
                     /translation="MDALQTTATDDANRVRAATIARAFADPKRLCVVERLASGERSVS
                     ELSRDVGCQVPNMSQHLAVLRAAGLVTTRRVGSTVLYRLADDRVLDAYRMLQQVAG"
     gene            complement(12010..13284)
                     /locus_tag="VFV59_01740"
     CDS             complement(12010..13284)
                     /locus_tag="VFV59_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324578.1"
                     /translation="MTDWRAFAARQYRPFRGLAVAQGLIGSLTGPAITIPLLLGLGAH
                     PALATFLAVLPALGTMAQRWLPAMLDRTDGNLRGLVVLFASIGEPRGLYLAAVVAMSA
                     AGWLPTWAAIALIGVISGVLGALGSIAYSLEQSWYQIVLPEDQRRLVTPRLGGISLGI
                     GSVVLLPVALVIDDVAAAIGAWAYVAPLLVSGVAGVSSLAVLHRLPRPGRIRVPRRTA
                     WDSEGHARLRRHGHVMTLALLSAGFSPFLSVYAMTVLGTGAGFAIAVSAISSGTLVLS
                     SLYVSSHLRGGSSSRLLRRSFLLRAAALFLGLAAHPINPFAPAVLVLIAILLAAGDTA
                     GQLAANERLLRLATGPDVLAFQSHFVVRQVGAYGLGNATSSLIMLLGGYPAFAILFVV
                     AGAGRYAAARATELSPREAVSTDTQAISTPAA"
     gene            13549..13713
                     /locus_tag="VFV59_01745"
     CDS             13549..13713
                     /locus_tag="VFV59_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324579.1"
                     /translation="MNILLDCAWCGHEVVFSVDETDDELVCSACNTRMAFAPDPVTTF
                     ALLYETAQAA"
     gene            complement(13786..14292)
                     /locus_tag="VFV59_01750"
     CDS             complement(13786..14292)
                     /locus_tag="VFV59_01750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5324580.1"
                     /translation="MADRPAPPSGALDTSKTYTARFKTKKGDIVVELYADRAPLTVEN
                     FVNLARSGFYDGTTFHRVIGGFMAQGGDPTGTGTGGPGYQFGDEFHPSLRHDSAGVLS
                     MANAGPGTNGSQFFITYGPTPHLDNRHSVFGKVTEGMDVLRSIRERDPQRDRQPGDRI
                     ETIEITES"
     gene            14363..15562
                     /locus_tag="VFV59_01755"
     CDS             14363..15562
                     /locus_tag="VFV59_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5324581.1"
                     /translation="MTRTLFTGATVVDGTGAEPAAADIVVEDGRIVEVGNGLDGDDRV
                     DLAGKAVLPGLFDCHTHVMFSTIDTMRIIQTPFSYRFYQAMHNLEATLRIGITTVRDA
                     GGADLGVKQAVEDGLIPGPRMHISLSMISQTGGHGDEWMPSGINLEIGPYPGVPRTIV
                     DGAEEMRRLVRELVRAGADVIKVAVSGGVLSPRDKPTHAHFRLEELQVLVEEATAAGI
                     WVMAHAQATPGIKNAIKAGIRSIDHGIYLDDEAIEMMLARGTWLVPTLVAPRGVIDAA
                     EAGASIPEASVAKAREVAEIHRGSFAKAVEAGVRIAMGTDSGVTPHGQNLRELALMVE
                     GGMTPMQAIVATTRSAAELMGLEDELGTLEPGKRADLVVVNGDPLDVATLAERIESVY
                     QDGARVV"
     gene            complement(15581..16201)
                     /locus_tag="VFV59_01760"
     CDS             complement(15581..16201)
                     /locus_tag="VFV59_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324582.1"
                     /translation="MRRLFPVPLLVLILTACGGGGSSPSASASVPPSSPPSESGTPSG
                     SAGPSASEAPIPSEDLGEFSCDFPIVQDATVPRANITDVRVGEHADYDRVVFEFEQGL
                     PEITLDRASPPFTQDASGMPITVDGTSFLRLTMRGGTKQTDSGTSSYDGPTNFDPAFS
                     TLVDLVEGGDFEAQSTWYFGLSTDACARVIQLDDENGARLVIDLEH"
     gene            16308..17402
                     /locus_tag="VFV59_01765"
     CDS             16308..17402
                     /locus_tag="VFV59_01765"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEU5324583.1"
                     /translation="MSTVERPVSPGLKGVVAGETALAMIDGERGRLQYRGYPIGELVE
                     HGTYASVAELLWTGEWRPDAHLACAPLPAEAVEVLRRVAPTSGAMDALRTAISTWGAL
                     AGMTWPPTVEQARAVTAVAPSALAAYARLRDGRDPVAPDPQLGLAAGFLYQLTGEPPD
                     EAAARALDAYFIVGAEHGFNASTFTARVIVSTHSDLASAVVGAIGALKGPWHGGAPSE
                     VVDQLHQMGTVDQAEAWIRATLERGERLMGFGHRVYRAYDPRAAALRGVAERLSGVAG
                     WLEKAVAVEEIALRVLAERKPDYPIKTNVEYYAAAVLQGVGLDPSLFPATFALARHAG
                     WTAHCLEQADVNVLIRPEVRYVGPAERHLG"
     gene            complement(17353..18639)
                     /locus_tag="VFV59_01770"
     CDS             complement(17353..18639)
                     /locus_tag="VFV59_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019969445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEU5324584.1"
                     /translation="MADAPGLPDQILADTLREERGRLTAALVRILGDWDLAEELVQDA
                     AVAALEHWPSDGVPRNPGAWLMTAARRRAIDRLRRDARYRERLRLLTAEVNHMDPGVA
                     TGGPIDADDRLRLLFTCCHPALNREAQVALTLKTIGGLETGAIARAFLVSEATVAQRL
                     VRAKRKIRDAGIPYRVPDLGELPARLGEVLTVLYLIFNEGYLASSGEAAERRDLARDA
                     EWLTSLIARLMPDEPEVLGLLALMRLHLARADARFAGDDLVLLPDQDRKLWDRAAIDE
                     AVALLRRAQRMGRIGTYQLQAAILAEHATAPSWADTAWDRIVALYDLLLRVEPTPVVR
                     LNRALAVAERDGPAASLAELEPLADRLGGWHLFHAARADMLRRLGRHRDAVAADRVAR
                     ELTDNPAELRLLDRRLADAGVSPGAAPPGPRSAPRA"
     gene            complement(18632..18979)
                     /locus_tag="VFV59_01775"
     CDS             complement(18632..18979)
                     /locus_tag="VFV59_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5324585.1"
                     /translation="MKYMMLIAGDEAPWEAQTDAEQAASYARVAAWWKEQREAGRIVG
                     GHELQPSSTATTIRVDREGRATVVDGPFAEAKEMLGGYAILDVPDLDAAIALAASWPE
                     PDTLEVRPLVTDG"
     gene            19078..19341
                     /locus_tag="VFV59_01780"
     CDS             19078..19341
                     /locus_tag="VFV59_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324586.1"
                     /translation="MEDKRSTADRVGGLEGADAAALGLTPEPESAPPGAHVDRDEDLV
                     PGADFGDRVPDEAKPWFTSLAAESDQAGVENEPLDAEEDVERG"
     gene            19357..20340
                     /locus_tag="VFV59_01785"
     CDS             19357..20340
                     /locus_tag="VFV59_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5324587.1"
                     /translation="MTQLDRPRPSTAAVIVALLVVYVVWGSTYLGIAIMIETMPPLLA
                     AGVRYGVAGVLMLGALVALARLRPAAAPLERPTGAQWRSTFVVGTLLLLGGNGGVVLA
                     EQFIPSGIAAVLVAMVPIWLAVLDAVVTRRRPSRLVVGGLVAGLVGVVVLLAPVEGLG
                     GIDPLGILLVVGASLSWAIGSLYARSAPMPRSGLLGTGMEMLAGGAVLVVAGSLLGDI
                     GRVDPGSISTASLLAMIYLIVFGSILAFTAYTWLVANVPVTTLGTYAYVNPVVAVALG
                     AIFLSEPITLRTVLAAVLILGAVIAMVSGRPREAEEPGPVPEAATLEPDAT"
     gene            20337..20624
                     /locus_tag="VFV59_01790"
     CDS             20337..20624
                     /locus_tag="VFV59_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324588.1"
                     /translation="MSRDAVHVRCQATDDGWACTVRVGDDAAATEHAVTVRRDDLEEL
                     VSGTTPEDVVEASFRFLLERESRESILRRFDLPVIGRYFPEYREEIRRRLS"
     gene            complement(20633..21133)
                     /locus_tag="VFV59_01795"
     CDS             complement(20633..21133)
                     /locus_tag="VFV59_01795"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEU5324589.1"
                     /translation="MTAGPELLTTFSGRAYRQTGVDTPAFPPDRPAVGEGRYHRPGEP
                     WPLYASLEPATVWAEWSAATRGAIDPRQERRRLWSVRAEDLRVVDLRRPEVRDALGVD
                     LGDLVGPRDAAQRLGARVRSLGADGLIAPSAADPDAWNLVVWPQAFGRVRAGRPRTMH
                     PQPPAP"
     gene            complement(21130..21456)
                     /locus_tag="VFV59_01800"
     CDS             complement(21130..21456)
                     /locus_tag="VFV59_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HEU5324590.1"
                     /translation="MTIRAAEPPRLIRLFPARFIARATGANVRTAERWRAGQAPQARY
                     RDRIGELQAILELLGRGMTDTAKRAWLEAPNAQLAWQRPVDRLAEGDFDAVRGAAESY
                     LVGDYV"
     gene            complement(21529..23097)
                     /locus_tag="VFV59_01805"
     CDS             complement(21529..23097)
                     /locus_tag="VFV59_01805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU5324591.1"
                     /translation="MLSTLMTLVAACTLAPPSETTPAEPTPVRPSSGASPSATPSPTP
                     SPSPTPDPEVEVPLAVVTGYIASTGAITLDEVQAAADTLLVPCGVRSYLDRTVAAGGD
                     CLEAEEIADRLNTAPQDQPLLALLPPSMVTPDVKVLPVDGADIFGGPDARAADYPITG
                     TAVGADPYDPDEIRTLMSLGDSCPDRGVAYAAITLGRGWDWVFGGGTAQYTAVYPNPV
                     PAGYVGNGFNIVDAVRTGNEGAVAELVSGADITLDDFECPVVDNWLVNDGVVFSIDPA
                     VLPRLRDTYGVDVATLAANHLFDQGVSGFLETLDKFEAAGIPTAGAGTNLDDALEPAL
                     VDVNGLTFGFVGFNEIPPEFAEATADSPGVAWLRDENVVDAAGRARSQGADVVVCVPQ
                     WWGGAEYHAEFRGDMRRQQEVFFDAGCDQVVGHGTHWSGPIEVTPDADGRYHVTMVSH
                     GNFLFGQEWSQQTQEGVTLELSFRGTDLVQARMHPYIMLEQAQTNLTDPETDGLYVLN
                     RIYEASGLCCGAAP"
     gene            complement(23124..23666)
                     /locus_tag="VFV59_01810"
     CDS             complement(23124..23666)
                     /locus_tag="VFV59_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324592.1"
                     /translation="MRSFPRSAALVAAALTTILVACGTTAPVSPTARGTDASPPAPSA
                     SASADPAAAAAYGTAICPLFDRIVNLDPRLAAIRAAGDEGGDMTGYEDELSALADELR
                     DIVNALDEVPTWGPGRRLQLEIMGTLHLIRAELLSINEDLTAVDTADAMAALPYIASQ
                     TMDREMARAVEGGLTCGTDE"
     gene            23782..24078
                     /locus_tag="VFV59_01815"
     CDS             23782..24078
                     /locus_tag="VFV59_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU5324593.1"
                     /translation="MTEQLPPPDALAFGSGTSDDHRDGIRWVDYRNISWNPVFCKRCD
                     ICVDICPKGTLVLRNDAIIEEDNCILCGLCERYCPDLAIEMIPEAVAAHAAKEK"
     gene            24075..24515
                     /locus_tag="VFV59_01820"
     CDS             24075..24515
                     /locus_tag="VFV59_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324594.1"
                     /translation="MSRRVIRYPTGWTLAVLPDAAAADAVAGELSALGVAADDLVVLA
                     GADAASRLERLGTSAGVAARVRRTVQFMTMDQLPDLHVYELALAREHPVIAVRVSDAD
                     ARRRAVEALRGHGGHFINRFGAWATEEIAPWRGEPLPVGQLMQR"
     gene            24520..25656
                     /locus_tag="VFV59_01825"
     CDS             24520..25656
                     /locus_tag="VFV59_01825"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU5324595.1"
                     /translation="MTRKLTQGNEAVFRGAVAAGANYYAGYPISPSTEILNVASGWAA
                     EHPEFRFLQAEDEIASANAIIGASLAGAKSFTATSGPGFSLMQEAVGYAQKVGVPCVF
                     VDVQRVGPATGMPTMPGQGDIMQVKWGSTGDYHPLAFYPNSVPEAFRMTVEAFNAAEE
                     SRSPVVVLTDTFVAHLNEVVDLDEIGREVQVVPRDIPALGDFVDEPRFFAGVLMYEHG
                     PNAGEPATADANEYLRQYYASKHRHLEVAGRYAYYEHGWNVDAEVLVICYGIVSRVAA
                     PLRDEFALFRPIRIHPILGDELRDITARYRDVVVVEANDGQYADLVELAIHRQVKRVP
                     LLGGRISLEAVRDGIDRVLSGGPSEPPIPPEPTEPQPRSPLGVG"
     gene            25711..27168
                     /locus_tag="VFV59_01830"
     CDS             25711..27168
                     /locus_tag="VFV59_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5324596.1"
                     /translation="MPEAPMAELGYKDRLKVELFPTIWCPGCGIGTIMMQLALTLDEM
                     GLNERNTIIVTGIGCTGRMGGYLKHEAVYTLHGRTLPVAEAIKTVRPEMNVIVVAGDG
                     DTASIGGNHLIHSIRRNAPVTVLCNNNEIYGLTGGQTGPTTPTGTKTLSSPAGNPNTP
                     INLQGLVRSSPHALYAKSTVYHQLHLRNAIREAIEWPGFSFVDITSQCIENNGRRIGF
                     ASANEMLTFYRKTYKRAPKDADHLAPFEIGIVYPAQHPARNGNGTAPAAETDGQRPEA
                     LPAQPTGPMASATDSPGEGGATTPEAATKVIEARTDDEKARRRAESVERAALMARLSP
                     DVKVRVAKREITLVQALSEAGIEIPEWARTGASAPDAPVPAGTVERSATGADPATEVK
                     PELDAQKAEAAAEGRDLIGGQPGGDTQPAAPRTPEDAAPASVQAPADSREAKRAESQR
                     KLALMQRLPSDLKLRVAKREITLEDAMHEAGIEPE"
     gene            27165..27785
                     /locus_tag="VFV59_01835"
     CDS             27165..27785
                     /locus_tag="VFV59_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5324597.1"
                     /translation="MTTKIALDGVGGQGVRVIAGVMGALLARMGKEVTVLFDYDSSVR
                     GSMSDAFLIFDDHPIENPVVEEADIMLKLADKGHLRIHGRTVVTDLGLHVPGAEQIPF
                     ASLGFQHFGKELFGNMIALGRLLRLANIEFDEPAIRESLPRRYQDENVAAIRFGYELT
                     PESIAAYAAAAPPSRFQMKDAATAPGGHPRGASVGIGTAADVGEGG"
     gene            complement(27814..28293)
                     /locus_tag="VFV59_01840"
     CDS             complement(27814..28293)
                     /locus_tag="VFV59_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324598.1"
                     /translation="MHWITTRPAAAGAILLVMAVALAGCFNERDENTNEGTNPSPGAI
                     AVEVRLTDDAIEMPSEIAGGAVEFEVSNAGASSHGFAIEGVEASLDELRPDQLDTLQV
                     ELQPGTYTVYSPVDGDRDAGLEVQLTVTEPATDGGAPLNDEGVGPSEEQQPIEGGGG"
     gene            28389..28742
                     /locus_tag="VFV59_01845"
     CDS             28389..28742
                     /locus_tag="VFV59_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEU5324599.1"
                     /translation="MSSDPVESNPGLYRVVMENERVRVLEYRDRPGDRTTPHGHPDSV
                     MVTLSSFRRRLVSGDQQAEVEIPAGVVRWLAAQEHLGENIGDSATHVVFVELKEPRPQ
                     GSTATAPGALGPSEG"
     gene            28749..29861
                     /locus_tag="VFV59_01850"
     CDS             28749..29861
                     /locus_tag="VFV59_01850"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU5324600.1"
                     /translation="MERGADALDLDVIRRAPKVLLHDHLDGGLRPRTVIELAQAAGYR
                     DLPTTDPDELARWFTLGADRKSLELYLEGFRHTVAVMQTREAIERVAFECVEDLAADG
                     VVYAEVRMAPELLTDGGLSLDEATDAMLAGFRRGMEGRPITIGLIITAMRQAARSVEI
                     AELAVRHRDEGVVGFDIAGPEAGYPPTRHLDAFHLIARENFHFTIHAGEGFGLPSIWE
                     ALQWCGAERLGHGVRIVDDIAVRPDGRVALGRLAAYVRDRRIPLEMCPTSNVHTGAAP
                     SIEEHPIDLLRRLRFRVTVNTDNRLMSGITLSSEFATLARTFGIGLDEMEWLTLNAMK
                     SAFWPFDGRLKIINAQIKPGYATLRAQQAAAELATT"
     gene            complement(29874..30755)
                     /locus_tag="VFV59_01855"
     CDS             complement(29874..30755)
                     /locus_tag="VFV59_01855"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5324601.1"
                     /translation="MQLTDLDHRPLVVAAVTPLSEDGSALDADAIPPYVAFLESRGAD
                     GIFACGTTGEGVLLTMDERRRAVEAFRAALSGILIVHAGAQTTADTVALAEHAREIGA
                     DAVAVIPPPYFALDADALTAHFVAAARAAAPLPFYCYAFTARSGYPLPVEVVRRIGGS
                     VDNLAGLKVSEAPFERVEPYLDLGLPVMIGSEPLIGAGLARGALGTVSGMSAAFPDVV
                     RAALDAGDEAAMARLRQLRGAMEASGQFIAAAKHVLGMRGLAVTEGMRAPLRPLNESE
                     AAALEGAVAEFLAPASV"
     gene            31000..31242
                     /locus_tag="VFV59_01860"
     CDS             31000..31242
                     /locus_tag="VFV59_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU5324602.1"
                     /translation="MTYVITEPCIGVKDASCVDVCPVDCIHATDDDPQYYIDPDECID
                     CGACEPECPVTAIFAEDAVPDDQVQYIQINADYFKR"
     gene            31242..31550
                     /locus_tag="VFV59_01865"
     CDS             31242..31550
                     /locus_tag="VFV59_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEU5324603.1"
                     /translation="MPRLIALYRPPEDPAAFDAHYRDVHDPIVRRYPKLAGVRITKLA
                     GVAGRPAPYYQMAEMIFETDADLAEALGSEPGMESARDLRNFATAGVELFIAPDEADG
                     "
     gene            31543..32502
                     /locus_tag="VFV59_01870"
     CDS             31543..32502
                     /locus_tag="VFV59_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5324604.1"
                     /translation="MAEPGSPVPTDPRELRYERISYERDTETGIATVTIQRPEVLNAL
                     DLPTLNEMAHAFQQASWDDRVAVVVVTGAGDRAFCTGADLDEQALMGSTSGQYWRWMG
                     AFIEMHDRLRQIGKPTIARINGVCVGGGQELQLACDLSVMVDDAYIRHVGPEHGSVPA
                     GGATQWLPLVVGDRRAREIVMLCEPVSPQQALDWGLVSRVVPRDQLDATVQELGIKLA
                     RKLPEALRYTKTQLNWWRDLVWSQTIQHARDWLAVHSTADETKEAVDAFHEKRKPRFA
                     ELRRLEAGEGRVCPACGATGLPAAHRFCGACGAELPDAWGAAT"
     gene            32499..>32872
                     /locus_tag="VFV59_01875"
     CDS             32499..>32872
                     /locus_tag="VFV59_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5324605.1"
                     /translation="MTQPTAPEELVLVERQPGQRTALVRLNRPKQLNALNGAVMDALC
                     VALEELDRDDEVRAIVVTGSERAFAAGADIGEMANATPIDMLRTNRIGQWDRIRRITK
                     PLIAAVNGWALGGGCELAMTLDL"
BASE COUNT         4606 a        11543 c        12150 g         4573 t
ORIGIN      
        1 ggcattgcgc tgtcggcggc cgccgtcacg tggtcggtcg gtgcgtggat ccaggcgcgc
       61 gcggcgggtg ccggcacgcg cggcacgctg gtcgccgccg gcgcgacgct catcgccgtg
      121 ggcatcggcc tcgcggcgct ggtgctgctg cccgagctcc ccgtcctggc cgcgaccgcc
      181 ggatgggcgg tcggtggact gggcatgggg ctggcctact cgatgctcgc gttcctgacg
      241 ctggagacgg ccccgcccgg cgaagagggc gcgagctccg cgtcgctgca gctcatgttc
      301 accctcggca cggcgttcgg ggccggtgcc ggcggcgcgg tcgtcgcgct ggccgatcgc
      361 ggtgcgctcg agctggtgga ggccctgggg gtcgtcgacg ccatgatggt cgtggtcgcg
      421 gtcaccgcga tggtcgtcgc cctgcgcgtg cccaggcgtc cagcaccgcg gaccgatgcg
      481 ctggtcgcga cgccgccacc ggtcccggct gcggagctcg gctgagccgt ccggaacgaa
      541 cgcgaccgac cgctgtcacg aacctggggg aggatggctg catgacaccg acgcgaagga
      601 tcctggccat cgacggcggg ggcgtgcgcg gcgtgattcc ggcggcgctg ctcgcgtcga
      661 tggagcgtgc cacgggtcgc gcgacgcgcg acgccttcga tttcgtggcc ggaacgtcga
      721 ccggtgccgt gctcaccgcc ggcctggtcg cgggcatccc cgcccagcgg ctggtgtcgc
      781 tgtacgtgga tcgggccgcg gaggtgttcc ggagcgtccc gctcctctcg ttcgtgcagc
      841 ggctggtcgc cggacaccag tacgacaccg cggggctgca ccgcgtcatc cgcgaggagc
      901 ttggcgcccc ggctgcggac tggcggctga acgacgcgcc ggtcgacctg ctggtgaccg
      961 ccaagcgcct ctcggacggg atgccgtggt acttcgtgcg cgatgacccg gccaacgccc
     1021 agcgtgcggg tgccatccga ttggccgatg ccgtcacggc gtcggcggcc gcgccgacct
     1081 tcttcgcgcc gtggaccatc ggcggcatcg gcgagctggt ggacggtggt gtcggcgtgg
     1141 cgggtaaccc ggtctaccag gcgtgcgtcg aggcgttcga gtacacggat cgctacctgc
     1201 ccggtgagac ggtcgtggtc agcctgggca cgggccgcgt cctgcagcgc gagcgcccga
     1261 cgtggctcgg cagctggatc ggctggctgc tgtcggagct gctgcgttcg ccggccgagc
     1321 agcagaccga gctcgtgcac cgccactacc ccgcggtggc gctgtcgcgc atcgacgtgg
     1381 agctgcccgg cgccatcggg atggacgccg ccgatcgcgt cgacgagctc cgcgcggtgg
     1441 gcgagcggct ggcgacgacg gtcgactggg aggcgctgct ggacggtcgc gacgagcggt
     1501 tccgcgtcac cggcgagcgc acgctgccgc gggagtacgt cagccgcgat cccggctgat
     1561 gcggaacgcg ggccgctcga acgtggtcga tgcagaccgc gagtgacgcg cggggagggc
     1621 tacaccacgt tcagctcgag gatgggcgcc tcgcgctcga tgcgctcgta gcccagcggg
     1681 gagaggtcca ggctctcgta tcggcccagc gtgatccact ccgccagtcc gcggccgacc
     1741 gcgggcgcct gctgcaggcc gtggccgctg aagccgttgg cgaacagcag gttcgcgatg
     1801 cgcgggtgcg gtccgaccac cgcgttgtgg tcgagggtat tgacctcgta gtgcccggcc
     1861 cagccgtcga gcaggcgcag gcgatcgaag gcgggaacgc gctgcgcgat cgtcggccac
     1921 acgaaggact cgaacggcgc gtagtccacg ctgaggtcga gcgtgtcggg gtcgggatcg
     1981 ttgccgtgcg gcgaaaagcc ggccaggtac gccggtccct ccgggcggaa gtagatgccc
     2041 gagggatcga tggtcaacgg cgcggcaccg agcatcgcgg gggactcgaa gtggtagacg
     2101 aggcgcttgc gcggccgcac cggaagctcg acgcccgcca tggcggcgac gtccgcggct
     2161 cgtggtcccg cggcgttcac cacccagtcc gcggcgatgg ccgagccgtc ggccagccgc
     2221 acgccggcca cgcggtcgcc gtcgaggtcc agggcggtga cctcgccgac gatctccgcc
     2281 acgccgagtg agcgggcctt gcgtcgaacg ccctggagca gggcgtaggc gtcgaaccag
     2341 ccctcgtcgg cgagcccgag ggatgcgccg gccagatcgt cgacgctcat ccaggggaac
     2401 cgcgccgcca gctcggacgg cgtgaggagc gtcaccggga cgtcccgggc gcgctgcacg
     2461 gcatggttgt gctcgagcgt ggcgagcccg gcatcggtcg ccaggaagag gtagccattc
     2521 tcgacgaagt ccagctgcgg cacctcgccg tcgacggcga ggtatcggtc cggatgcttg
     2581 acgacgtcaa ggccgaaccg gctgatgtcg atgttcagcg gcgtgctgaa ctgcagccgg
     2641 atgctcgcgg ccgacagcgt cgtggaggag gttcggaagg tcgggtcacg ctcgaccacc
     2701 acgatccggc cgccgaagtc gggcctcgac gccaggaacg tcgcgacggc cgacccgacg
     2761 atggcgccac cgacgatgac gacgtcggcg cgctccgagc tgctcatcgg ccgatgctag
     2821 cggctcagcg gtcgggcagg ctcccggtct cggggtccgc ctggccctcg cgctggccgt
     2881 aggccgcgtc cgggctcgcg ccgaactcgt cgctcgccgc ctcggggtcg accggctgct
     2941 tcatcggtcc gtcggggcgc ggatcacgga ccgcgggatc ggcggcgccg acctcgcgcg
     3001 gctcctggtc ctggccgtgg gtctcgccgc tcgtgacgtt cgcgttctgg tccgtcatgg
     3061 gtgcctctgc tgcgggggtt gcctccggcc tgctgaaggc aggttcgatg ccagtgacgg
     3121 ccccgggacc ttcgttcgat tggcggctcg tcgccggcac gggacgatcg gtccatgctc
     3181 tccgcgtcct ggccgagccc ggttcgcctg ccgcgtcccg tccccccgcc gcacgtccgg
     3241 cgacgggtgc gagcggccga gcgccgcgcg atccgccgct cggtggtcat ggcgtcgccg
     3301 ctggtcgcgg tgttcttcgt ggtcgacgtg ctgctgcgcg tgtcgacgca ctccaccggt
     3361 gcgctggcct tcgcgccgtg ggagctcgcc ggcgccgcct tcgcgctggc gatggcggtg
     3421 ttggcgcgcc gcagcctggt ccggtcggag gccgccgccc tggccgtggt cgcggtgacc
     3481 gtcgcaatcg cgctgctggg catccagctc gtgccggacg gccgcatgct cgcgacggcc
     3541 cagctggcca tcctggtcgt cgggaccggc ctcttcatcc cctggtcaca ttggtggcac
     3601 gtgagcgcca tgctcgtcag tgtcaccatg agcgccgcct tcgtcgtcgc gccgtggacg
     3661 ggcggcctgc cgccacaccg gggcatgaac ctcgtcgtgg cggtcatgag cgccgcgatg
     3721 atcagcctca tcggccacgc gacgtggcag cgacggctcc gcgccctgct ggtccagcag
     3781 ttcgccctgc gccacctgag ccgatacgcg cagcgccagg aggcgcacgt gaccgagctc
     3841 aaccgtgagc tcaacctcgt ggcgcggcgg gatgccctga cgggggtcgg gaaccgcctg
     3901 gcgctggacg aggccctgga tcggctgctc gcgcgggagg atcggctgcg accgatgcgc
     3961 ttcgcgttgg cgctgttcga catcgatcac ttcaaggcct acaacgacga gcacggccac
     4021 ctggcgggcg acgccgccct cgaacggctc ggcgccatac tgcggcgcgc gacgcgcaac
     4081 gacgacctcg ccttccgcta tggcggcgag gagttcctgg tcctgctgcc ggacgtgagc
     4141 ctcaccgatg cgatcgcggt cgcggagcgg atccgggtcg ccgccgagga ggacgagacg
     4201 cgcgcgctcc cgccgttcac ggtttcgggt ggagtcgcgc tgtgcgaccc ggccgacggc
     4261 cacgacgccg agccgttgct gcgccgcgcc gatgccgcgc tgtacctggc caagcgcgcc
     4321 ggccggaacc ggatcgccgc cgatgagctg tccgtggcga tgcagcgcgc ggatctcgcc
     4381 tccgcctgag gctctgcgcc ctcagtaggt cgggtggtcg agcagccacc ggccctcgac
     4441 gaggaccagc tgccagtagc cgacgaactg ccgagtcgcg ccggagtcgt aggtctcgac
     4501 gaagttcgcc tgcaccgttg cggtcgtcgc ggtccggctg gcgacgaaca gctccgtgaa
     4561 ctccaccgag gccgtctccg cgaaccgctc gtcgaggttc tcctgccgcg ggtactcggc
     4621 cttcatgcgg tcgctccaca acgcgtaggc acggtcgaag tcgccggcgg cgacggcgcc
     4681 atagaaggcc gtcaccgcgt caaccggctc cgctgccgag gtcacgatgg acgttggcgc
     4741 gggcgtgcgt ggaggcggcg gcaccggggt cgggccgagg gcgaccgcta cgggcgtcgc
     4801 ggtctcgacc ggcgtgtcgc ggggggtcgg caccggcgtg gcggtcacgg tcggttgggg
     4861 cgccgtcgcg gtgggggacg cgcggttgag cacgaagccg gcgggcgtcc cgaccacgat
     4921 ggaaggcagg gcaacctcat cgcgcgcgac accggcgtca cggctccacc aggtgatcgc
     4981 gagggccagc accgcggcga aggcgagcac cccggccatc gcgatcgcca cgccacgccc
     5041 gcctctccgg cgtcgagtgg cttccgccga aggggcttgc gtggctccgg gctgctcggg
     5101 cggggatgga ctggtggtcg gggtccgggt cgtcgcgggc ttcgcgcggc gggtcgatcc
     5161 gctcgatccg cgggcgcgtg ctgccgacct ccggttctgc ttcgttctgg gcacgcgtgc
     5221 tcgtgagcac ggcgcgttcc acgctgccaa gatgaccggt gtgaagttcg gactgcagat
     5281 caaccagttc acctggccgg gaggtgttgc cggcatggcc ccgacgctcg cgcgcgtcac
     5341 gcgtaccgcc gacgacgcgg gattcgactc catctgggtg atggaccact tcttccagat
     5401 ccggggtctc ggccctcccg aggcgccgat gctcgagggc ccgacggcgc tcgggttcat
     5461 ggccgcccac tcggagcgcg ctcgcctggg gctgatggtc ggcggcgtcc actaccgcgc
     5521 gccgggactg tggatcaagg ccacgaccac gctcgacgtg ctgtccggag gccgcgcctg
     5581 gttcggcatc ggcgcggcct ggaacgagca ggagtcacga gggctgggct tccgcttccc
     5641 gccgctccgc gagcgcttcg agcagctcga ggacgtgctg cgcatggcgc atgcggcgtg
     5701 gagcggcggc accggtaccg gcgagcgctt cgaggggacg catgtcaccg ccgagcgcat
     5761 cctcaactcg ccccaggccg tcagccggcc gcgcgtcccc atcctggtgg gcggtggcgg
     5821 ggagcggaag acgctgcggc tggtcgcgca gtacgccgac gcgtgcaaca tcttcggccg
     5881 ctcgccggcg ttcgtccgcg acaagctggc cgtgctgcgc gggcactgcg agcgcctggg
     5941 ccgcccgtac gacgagatcg agaagaccgt tctcaccagc gtcgatccgg ccgaggaggc
     6001 gtcagccgcg acggtcgagc gcttcggcgg cctcgccgag gccggtgccc agcacatcat
     6061 cttcagcgtt cgaggcgtcg gggccgaccc cgggcgcctg gagcggctgg cgagcgaggt
     6121 cttcccgcag ctccgctgag cggccggagc tcagcgaacc ggcgctggcg agggcccgtc
     6181 cgcgagcacc gcccgcgccg ctcgttcggc cagcagcatg atcgtcagca tcgggttcac
     6241 gccgagggcg gtcgggaaga gcgaggcatc ggcgacgtac acgccgggga cgagtgcgcc
     6301 ccgctggtcc gcgcgcagcc gcccgtgggg atcgcaggcc gactggcggg gatcggctcc
     6361 ggcgcgcgcc gagcccatct ggtgtgctga gaagagcgcc agccggttgg ggccggtgtc
     6421 caccgtcgcc agccgctcca ggtaggcgtc gaagtcctcg ccacgccgcc agcgttcgcc
     6481 ctccgtcgtg agggcgacca tgtcgtccgc cccgccggcc tccgccatcc gggccatggc
     6541 gaccagtgcc gctcgcgcgg ccacccggtc cgcggtggcg agccggtagc cgatcgaggc
     6601 gcggccactg cgacggcggg tgatgatccc cgcgctgcgg tcccggagga tgccgatcag
     6661 cggtgcgagg cggtcgagac gggacaggag ctcggtcccg gcgtcgcggc ccagccacgg
     6721 aagcgcggcc gcggcgaggc cggggtgcgc gggcgccgac tcgatcagga tcgagcggtc
     6781 cggacccagg aactgcgcgg atcgggcggc ctgggtcggc ccctcccaca ttgccacgcg
     6841 gtgggggagc tgggccgcga tcaccgggct cgcgtgcagg tgcaggttgc gcccgatctg
     6901 cgggtgatcg aagccgctgc cctggagcag gatcggcgtg cgcagcgcgc cgccggcgac
     6961 gaccacctgc ggggcgtgga cgacgaacgg ccggccgccg gccagccgac cacgaacgcc
     7021 ggcggcccga ccatcgccga gcagcacgcg cgtcacctgc gcctcggcca gcaggcgggc
     7081 gccggtcccc tgcgcagcga cgaggtggac ccgcgcggtc gagcgcttgg ccccgcggtg
     7141 gcagccgaag ctgcaggcgc cgcagtcggt gcacggcccg gcgttgcggc gcgtcgtgtc
     7201 cgcgtcccag ccgagggcgc gagcgccgtc caggatgagc tggtccttgg gcgggacctc
     7261 ggtcggcggc atcaggccca gctcgtcggt gagacgggcg aggtcggcgt cggccgtcgt
     7321 gcctcgcagc ttgcccaggc cgtgttcgcc ctcccactcg tccacgaccc actcgggcgg
     7381 cgggatggac gtggtccagt tcaccgtcgt ccccccgccg acggtggcgc ccgccaggat
     7441 ggtgaccgat gcgtcgtccg ggccggtggt gccccggtcg aggtacatgt cgcgccaccc
     7501 gatggcctcc gaagaggtga ccccggcggc ggggacgtgc cggccggcct cgacgaccag
     7561 gacggagcgt ccggcggacg ccagccgcgc cgcgacgacc ccgccgccgg cccccgatcc
     7621 gacgatcacc acgtcggcct ccaggcgcag cggctcggca ccctcgcgcg ggacctccag
     7681 cggacgcacc tccgggatgt cgacgtccac aacggcggcc cggtgcggga cgtagccgat
     7741 ccgctcccac atcgggttcg acggttgggc gggatcgtcg ccgggatcgg cgtaggccag
     7801 gaacatcccg atgcgcttga gcgcctggaa cgcggtcctg cgctgcggaa tgcgcgacgt
     7861 ggcccacgcc cgaaggatcc gctcgcgggc cgcctgcggc gcgcggctga acggggtgag
     7921 gcccgcgcca tcgagcgcgg tgacgatcgg gagctccagc gcgcgcagga cgagccccag
     7981 ctgggcgacc tcgctgcggt cacgggtggc ggagagcgtc tccaccgcgg tctcggcgcg
     8041 ccgccgcgcc tcctcgggcg tcatcggtgc cagcgtggcg aagacttcgg ccagcaggcc
     8101 gaccggcagc agctccacgc cggtctgccg cctcaggcgc cgatgagctc ggacgcgacc
     8161 tcgtcgatcg ggcgcacggc ccgctcgccg ctcgcccggt ccgtcacttc caccccgccc
     8221 gccgccagcg cgcgcggact gacggtgagg atcgtcggca tgccgagcag ctccgcgtcg
     8281 gtgagcttga cgccgggcga ctcgtcgcgg tcgtcgtaca ggaccgatac ccccgcatcg
     8341 gtcagtcgct cgtagagcgc atcggctgct tcgccaacgg cggcatcctt cgccgcaccg
     8401 atggcgacca ggtgcgcgcg gtagggcgcc acctcggcgg gccagacgat gcccttctcg
     8461 tcgtggtggg cctcgacgat gcacgccatg gcacggccca gcccgatgcc gtacgacccc
     8521 atgatcgggt gacgccggct gccgtcctcg gccaggtagg tggcacccag ggcgaccgtg
     8581 aagtcgctcc ccagcttgaa gatgttgccg acctcgatgc cctgggccag ccgcacgggc
     8641 tgcccgcagt ccgggcacgg atccccggcg cggacgtcgc tgatgtcgtc gacgacgtcc
     8701 ggctggtaat cgcggggcac gttgacgttg cgcaggtgca cgccggggcg gttggcgccg
     8761 gccaccaggt tcggtgactc ggccaccagc cggtcgacca cgaccaccgt gtcgtgcgcg
     8821 cctacgggcg agccgtagcc gggctccatg ccggcgcggc gaatctcgtc ggcctgcgcg
     8881 ggacgcgtgc cgccgatcgc cttgaccgcg ttgaccagct tcgtctcgtt gacctcgaag
     8941 tcaccgcgca cgatggccgt gaccagccgg ccatcgccgg tgacgaagaa ggcggccttc
     9001 gccgtcttcg ccgtcggaat gcccaggtag tcggccaggg cggcgatggt ctccgtgccc
     9061 ggtgtatcga cgtcctcgag gggcatcggc tcctcggtcg tgtcggcgac gaccgcgttg
     9121 ggcgcgatct gctggttcgc cgcgaagccg caggcgtcgc acaggacgag cgtgtcctcg
     9181 ccgtggggat tgaggaccat gaactcgtgg gcgccggtcc cgcccatgat gcccacgtcg
     9241 ctcgacacgg cgatggcgtc gaggcccagc cgctggaaga tgcgcgtgta cgcctcgtag
     9301 tgccggtcgt agctgcggtc cagcccggcc tcgtcggcgt cgatcgagta gctgtccttc
     9361 atgacgaact cgcgcacgcg gatgaggccg ccgcgggcgc ggggctcgtc gcggaacttc
     9421 gtctggaagt ggtagacgat ggcgggcagc tgccggtagc tcctgaccag gtcacgggtc
     9481 aggtcggcga cgacctcctc gtgggtcatg gccagcacca tgtcgcgccc ggatcggtcc
     9541 ttgaagcgga ccatctcggg gccgatcttg gcgtagcgcc ccgactcctt ccacagctcg
     9601 gcgggatgca cgaccggcat ctccatctcc tgcccgccga tgcggtcgat ctcctggcgg
     9661 atgacctcct cgatggcgtg ctggacgcgg tagccgagcg gcagcagtga gtagatgccg
     9721 gcgcccagct gccggacgta gccggcgcgc accagcagct tgtgcgagac catctcggcc
     9781 tcggccggat cgtcacggag ggacgcgaag aagagctgtg acagccgcat ggggagccga
     9841 ttctaccggg tggcgggcac cggctccgcc gaaggccctc ccacgaggtg ctcggagtcc
     9901 cggagccagc gcagcggccc gagcgcaacg agcagcagtc ccgccgatgc caggaggagg
     9961 ccgtcgatgc ccagccactc ggccgccccg ccgcccgcca gcgagccgac cacctggccg
    10021 acggcgagga acacggagta caggcccatg atcgccccgc ggtcggccgg gtgctcctcg
    10081 ctgatgtccg ccagcatccc gagcgccgcg ggcgtggcgc cggccaggac gaagagcccg
    10141 cccgccatca ccaccccggc gaccagctgg gccggcagcg gccacgcctc ggcgtggttg
    10201 atcgcgaaga tcgcgaggac catggccagg ccgccgacga tgccgatggc catgatcgtg
    10261 gtgcggcgga acgccttgaa gcggttgccc cagtaccaga gcccggcgaa gaagacgacc
    10321 agcgcgaccg ccagtccgat gctgacctgc cacgactcgt agccgcccat cagccgctgg
    10381 tcctcgaagc ccggtcgcgg ctcgttgacc agctggaaga tcgactgact ggtccaggcg
    10441 ccgatgaacg cgttgaccgc gatccaggtg ggcgccagga gccacaccga tcggctgacc
    10501 aggaccgcgc ggtagcggct ccaggagaag ccgcgggcgg tggccgccaa gcccccggcc
    10561 tcgatctcct ccgcctcgcc ctcggaggct cgcccggggt cgacggtcac gccgtagcgg
    10621 tagatcagga agctgatccc gtacagcccg gcgttcagca ggaacgcgtt gcgatccagg
    10681 acctgccaca ggccgcccgc cgcgaccagg ccgagtccga gcccggcgag cgtggccgcc
    10741 tcgaaccgcg ccacggtgcg ccctcgcagc gcctcgtccc gcgaggtggc cagcgcgatg
    10801 aagccgagga tcgagggaat gctggccgcg gccgctgccc cttcgagcca gcgcgtgacg
    10861 cccagcagca acaggtcggt ggtgaggaag gtgaggacca cggcgacggc gccgaagatc
    10921 gggccgtact gcatgacccg ccgggagccg aggtggtcgg agatcgcgcc gaacagcggc
    10981 gagaggacga gctcggcgag gaagaaggcc gcggtcagca gcccgacctc gatggcgctc
    11041 accttcggcg cgccgaaggt ttcgagctcg gcgaggtaga agaccagcag cgctccggtc
    11101 aggcccgtgc tgaagcggag cgtgaaggtg ccgagcagga cggcgaagat ggagcgacgc
    11161 atcggccgct cagtatgccc gggctcggtt gggtgacgga taccaaacgg gcgcgccctc
    11221 gcgggcgcgc ccatcggcca tggagggggt ggctagctgt tggagaagcg cgagagcccg
    11281 aaggcctcct ccagcgcctc cgccggacgg aagccgacgg ccgcgcgggg cgcctcaccg
    11341 ggcgcgaaga tcgcgacggt cgggatgctc atgatcccgt acttcatggc ggtctgcggg
    11401 ttggcgtcga cgtccagctt gcgaacctcg acgctgccgg cgtacttgct cgccagcttg
    11461 tcgatctccg gggccaccat gcggcacggt ccacaccact cggcccagaa gtcgacgatc
    11521 accggcttct cgctctggag cacgtcggtg tcgaaggtgg cgtcggtgac ctcttgtacg
    11581 ttggacatcg gttcctctct cgttgagtcg cgtgacgacc ggcgtcagcc ggcgacctgc
    11641 tgcagcattc gataggcatc gaggacgcga tcgtccgcga gccgatacag gacggtgctc
    11701 cccacgcgtc gtgtcgtgac caggccggcc gcacgcagca ccgcgaggtg ctggctcatg
    11761 ttcgggacct ggcaccccac gtcgcgcgac agctcgctga cgctgcgctc accactcgcc
    11821 agccgctcca cgacgcagag gcgcttcgga tcggcgaagg cacgcgcgat ggtggcggcg
    11881 cgaacccggt tcgcgtcgtc ggtggcggtg gtctgcaggg catccatcgc cggccatgct
    11941 ctcatggttt gcgcaattac gcaagtcccg aaaggtcgcg gattcggagc cgatgcgcgc
    12001 cggtgcgcct caggccgcgg gcgtggagat cgcctgggtg tcggtcgaga ccgcctcgcg
    12061 cggggagagc tcggtcgcgc gcgcggcggc gtagcggccg gcgcccgcca ccacgaagag
    12121 gatcgcaaag gccggatagc cgccgagcag catgatcagc gacgacgtgg cgttgccgag
    12181 gccgtaggcg ccgacctggc gcacgacgaa gtggctctgg aaggcgagga cgtcgggtcc
    12241 ggtcgccagg cgcagcagcc gctcgttggc cgcgagctgt ccggcggtgt cgcccgcggc
    12301 caggaggatc gcgatcagca ccagcacggc gggcgcgaac gggttgatcg gatgggcggc
    12361 gaggcccagg aacagcgcgg ccgcgcgcag caggaacgag cgacgcagca gccgcgacga
    12421 cgacccgccg cggaggtggg agctcacgta gagcgagctg agcacgagcg tgcccgagct
    12481 gatggcgctg acggcgatgg cgaagccggc gcccgtcccg aggacggtca tggcgtacac
    12541 ggagaggaac ggcgagaagc cggcagacag cagcgccagg gtcatgacgt gcccgtgccg
    12601 ccgcagtcgg gcgtgcccct cgctgtccca cgcggtgcgg cgggggacac ggatgcgacc
    12661 ggggcgcggc aggcggtgca ggaccgccag cgagctcacg cccgcgacgc cggacaccag
    12721 gagcggcgcg acgtaggccc acgcgccgat ggcggccgcc acgtcgtcga tgactagcgc
    12781 caccgggagc agcacgaccg agccgattcc gagggagatg ccgcccagtc ggggcgtgac
    12841 cagccggcgc tggtcctcgg gcagcacgat ctggtaccac gactgctcga ggctgtaggc
    12901 gatggagccc agcgcgccga ggacgccgga gatgacgccg atgagcgcga tggcggccca
    12961 cgtcggcagc cacccggcgg cgctcatcgc cacgaccgcg gccaggtaca ggcccctggg
    13021 ctcgccgatc gaggcgaaca gcacgaccag gccgcggagg ttgccatcgg tgcggtcgag
    13081 catggcgggc agccagcgct gggccatggt gccgagggcg ggcagcacgg ccaggaaggt
    13141 ggccagcgcc gggtgcgccc cgaggccgag cagcaggggg atggtgatgg cgggcccggt
    13201 caggctgccg atcagcccct gggccacggc gaggccgcgg aacggccggt actggcgcgc
    13261 ggcgaaggcg cgccaatcgg tcatggcagc ggagagggtc gggttgggac gtggacgcag
    13321 ggaaggcatg gaccgtacgc gcgaagggat gaggggttga acggctcggg atcgcgcgta
    13381 ctcccgccag accggctccg gcggacatcg gtgcagggcg tcccggccgt gcatccgacg
    13441 tcgcatagcc tatcccatcc gccgtgcgcg gtggctgggt ggttcgtact ccggcgggta
    13501 ccaatcgcga cacgcgagtt gtcacgaacc gcgcccagca tgcgcggcat gaacatcctt
    13561 ctcgactgcg cgtggtgcgg ccacgaggtc gtcttctcgg tcgacgagac cgatgacgag
    13621 ctggtgtgca gcgcctgcaa cacccggatg gccttcgcgc cggacccggt gacgaccttc
    13681 gccctgctgt acgagacggc gcaggctgcc tgagccccga ccgacccggt gggggagccg
    13741 gcgggcggca ctgcccgccg gcggtcggtc ggacctgcga tgcgcctagg actcggtgat
    13801 ctcgatcgtc tcgatccggt cgcccggctg gcggtcacgc tgcgggtcgc gctcgcggat
    13861 ggaacgcagc acgtccatgc cctcggtcac cttcccgaac acgctgtggc ggttgtccag
    13921 gtgcggggtc gggccgtagg tgatgaagaa ctggctgccg ttcgtgcccg gtccggcgtt
    13981 ggccatcgac aggaccccgg ccgagtcgtg gcgcaggctt gggtggaact cgtcgccgaa
    14041 ctggtagccg gggccaccgg tgccggtgcc ggtcgggtcg ccgccctggg ccatgaagcc
    14101 gccgatcacc cggtggaagg tggtgccgtc gtagaagccg gagcgggcca ggttcacgaa
    14161 gttctcgacg gtcagcggcg cgcggtcggc gtacagctcg accacgatgt cgcccttctt
    14221 ggtcttgaac cgcgcggtgt acgtcttcga cgtgtcgagg gcgccggagg gcggcgccgg
    14281 acgatcggcc atcggatctc cttggggggt ggatgcgtga ccgatgctac cggagcgggc
    14341 cgtcgctggt agcctcgacg tcatgacacg cacgctgttc acgggcgcca ccgtcgtcga
    14401 cgggacgggc gccgagccgg cggctgctga catcgtggtt gaggacggcc ggatcgtcga
    14461 ggtcggcaac ggcctcgacg gcgacgatcg cgtcgacctg gccggcaagg cggtgctgcc
    14521 ggggctgttc gactgccaca cgcacgtgat gttcagcacc atcgacacga tgcggatcat
    14581 ccagacccca ttcagctacc gcttctacca ggcgatgcac aacctcgagg cgacgctccg
    14641 catcggcatc acgacggtcc gcgatgcggg cggcgcggac ctcggggtga agcaggcggt
    14701 cgaggacggc ctgatccccg ggccgcgcat gcacatcagc ctctcgatga tcagtcagac
    14761 cggcggccac ggcgacgagt ggatgccgtc cggcatcaac ctggagatcg ggccgtaccc
    14821 cggcgtgccg cgcaccatcg tcgacggggc cgaggagatg cgccgcctgg tgcgagagct
    14881 ggtccgtgcc ggcgccgacg tcatcaaggt cgcggtgtca ggcggcgtcc tctcgccgcg
    14941 cgacaagccg acgcacgcgc acttccggct cgaggagctg caggtgctgg tcgaggaggc
    15001 gaccgcggcc ggcatctggg tcatggcgca cgcccaggcc acgccgggca tcaagaacgc
    15061 catcaaggcc ggcatccgct ccatcgacca cgggatctac ctggacgacg aggccatcga
    15121 gatgatgctg gcccgcggga cctggctggt gccgaccctc gtcgcaccgc gcggcgtgat
    15181 cgacgcggcc gaggcgggcg cctccatccc ggaggcatcg gtggcgaagg cgcgcgaggt
    15241 ggcggagatc caccgcggct ccttcgcgaa ggcggtcgag gccggtgtcc ggatcgcgat
    15301 ggggacggac tcgggcgtga cgccgcacgg ccagaacctg cgggagctgg ccctgatggt
    15361 cgagggcggg atgacgccga tgcaggcgat cgtcgccacc acgcggtccg cggcggagct
    15421 gatgggtctg gaggacgagc tcggcacgct ggagccgggg aagcgggccg atctcgtcgt
    15481 cgtgaacggg gatccgctcg acgtggccac cctcgccgag cggatcgaat cggtctacca
    15541 ggacggcgcg cgggtcgtgt gacccgtcgc cgttccgggg tcaatgctcg aggtcgatga
    15601 ccaggcgagc gccgttctcg tcgtccagct ggatcacccg cgcgcaggcg tccgtgctca
    15661 ggccgaagta ccaggtgctc tgggcctcga agtcaccacc ctcgaccagg tccaccagcg
    15721 tcgagaaggc cgggtcgaag ttggtcggac cgtcgtagga gctggtcccg gaatcggtct
    15781 gcttggtccc gccgcgcatg gtcaggcgca ggaacgaggt tccgtcgacg gtgatcggca
    15841 tgccggaggc gtcctgggtg aagggcggag aggcgcggtc gagcgtgatc tccggcagac
    15901 cctgctcgaa ctcgaacacg acccggtcgt agtcggcgtg ctcaccgacg cgaacgtcgg
    15961 tgatgttcgc gcgtgggacc gtcgcgtcct ggacgatcgg gaagtcacag ctgaactcgc
    16021 cgagatcctc ggaggggatg ggcgcctcgc tggccgacgg gccggcggac ccgctcggcg
    16081 tgccggactc cgatggcggg gacgacggcg ggaccgatgc gctggcgctc gggcttgaac
    16141 cgccgcctcc gcaggcggtg aggatgagga cgaggagggg aacggggaag aggcggcgca
    16201 tggcacggct ccttgtgatc gtggcgatcg tgtgatcgat gcgttggctg caggttggac
    16261 gcaccgcacc gcggcgtcgt taccagcctc ggcctagact cggacctatg tccaccgtcg
    16321 aacggcccgt ttcgcccggc ctgaagggcg tcgtggcggg cgagaccgcg ctggcgatga
    16381 tcgacggcga gcgcggccgc ctgcagtacc gcggctaccc gatcggcgag ctggtggagc
    16441 acggcacgta cgcgagcgtc gcggagctgc tgtggacggg cgagtggcgc ccggacgccc
    16501 acctggcgtg cgcgcccttg cccgccgagg cggtcgaggt gctgcgccgc gtggcgccga
    16561 cgtcgggcgc gatggacgcg ctccgaacgg cgatctcgac gtggggtgcg ctggcgggaa
    16621 tgacgtggcc acccaccgtg gagcaggcgc gtgcggtgac ggcggtggcg ccctcggcgc
    16681 tcgccgcgta cgcgcgcctg cgggacggcc gcgatcccgt cgcccccgac ccgcagctcg
    16741 ggctcgccgc cgggttcctg taccagctca ccggcgagcc gccggacgag gcggcggccc
    16801 gcgcgctcga cgcctacttc atcgtgggtg ccgagcacgg cttcaacgcc tcgacgttca
    16861 cggcccgggt gatcgtgagc acgcactccg acctggcgtc ggccgtggtg ggcgcgatcg
    16921 gcgccctgaa gggtccgtgg cacggcggcg cgccgtccga ggtcgtcgac cagctgcacc
    16981 agatggggac ggtcgaccag gcggaggcct ggatccgcgc gaccctcgag cgcggcgagc
    17041 ggctgatggg gttcgggcac cgcgtgtacc gggcctacga cccgcgcgcc gccgcactgc
    17101 gcggcgtggc ggagcggctg tcgggggtgg ccggctggct cgagaaggcg gtggcggtcg
    17161 aggagatcgc gctgcgcgtc ctggccgagc gcaagccgga ctacccgatc aagacgaatg
    17221 tcgagtacta cgccgccgcc gtcctgcagg gggtcggcct cgacccgtcg ctcttccccg
    17281 cgaccttcgc gctcgcgcgc cacgccggat ggacggccca ctgcctcgag caggcggacg
    17341 tgaacgtcct gatcaggccc gaggtgcgct acgtgggccc ggcggagcgg cacctgggct
    17401 gactccggcg tccgcgaggc gtcggtcgag cagtcgcagc tcggccgggt tgtcggtcag
    17461 ctcgcgcgcc acgcggtcgg cggccacggc gtcgcggtgc cggccgaggc gacggagcat
    17521 gtccgcgcgg gcggcgtgga agaggtgcca gccgcccagc cgatccgcca acggctccag
    17581 ctccgccagc gaggccgccg gtccgtcacg ctcggccacg gccagggcgc ggttgagccg
    17641 gacgacgggc gtcggctcga cgcgcagcag gaggtcgtag agcgccacga ttcggtccca
    17701 cgccgtgtcc gcccagctcg gcgcggttgc gtgctcggcc aggatggccg cctgcagctg
    17761 atacgtgccg atgcgcccca tgcgctgggc ccgtcgcagg agcgcaaccg cctcgtcgat
    17821 cgcggcgcgg tcccatagct ttcgatcctg gtccggcagg aggacaaggt cgtcgcccgc
    17881 gaagcgggca tcagcgcgcg ccaggtgcag ccgcatcagg gccagcagac cgagcacctc
    17941 cggctcgtcc ggcatcaggc gcgcgatgag cgacgtcagc cactcggcgt cgcgcgcgag
    18001 gtctcggcgc tcggcggcct cgccggagga cgccaggtat ccctcgttga agatcaggta
    18061 caggacggtg agcacctccc ccagccgagc ggggagctca ccgaggtcgg ggactcggta
    18121 cgggatgccg gcgtcgcgga tcttgcgctt ggcgcgcacc aggcgctgcg cgacggtcgc
    18181 ctcggagacg aggaaggcgc gcgcgatggc gccggtctcc agcccaccga tggtcttgag
    18241 ggtgagggcc acctgggcct cgcggttcag ggccggatgg cagcaggtga agagcagccg
    18301 gagccgatcg tccgcatcga tcggtccgcc ggtggcgacg ccagggtcca tgtggttgac
    18361 ctccgcggtc aggagccgga ggcgttcccg gtagcgcgcg tcgcggcgca gccggtcgat
    18421 cgcgcgacgg cgggccgccg tcatcagcca ggcaccgggg ttgcgcggga cgccatccga
    18481 cggccagtgc tcgagcgccg cgacggccgc gtcctggacc aactcctcgg ccaggtccca
    18541 gtcgccgagg atgcgcacca gcgccgcggt gagccgtcca cgttcctccc gcagggtgtc
    18601 ggccaggatc tggtccggga gcccgggggc gtcagccatc cgtcacgagg ggccggacct
    18661 cgagcgtgtc gggctccggc catgaggctg ccagcgcgat ggcggcgtcg agatccggca
    18721 cgtcgaggat ggcgtagccg cccagcatct ccttggcctc ggcgaacggg ccgtcgacga
    18781 cggtcgcgcg gccctcccga tcgacgcgga tggtcgttgc cgtgctcgag ggctggagct
    18841 cgtgaccgcc gacgatccgc ccggcctcgc gctgctcctt ccaccacgcc gcgacccgcg
    18901 cgtagctcgc cgcctgctcc gcatcggtct gggcctccca cggggcctcg tcgccggcga
    18961 tcagcatcat gtacttcatc ggtggctcct cactcgagct tggtctgcct tcaccgatga
    19021 cgacgatccg gcgacggccg gatcgacatc ggtaccgatt ctgcatcgat cggcggcatg
    19081 gaggacaagc gatccaccgc cgaccgcgtc ggcggcctcg aaggagccga tgcggccgcc
    19141 ctgggcctga cccccgagcc ggagtccgcg ccaccgggcg cgcacgtcga ccgggacgag
    19201 gatctcgtcc ccggcgcgga tttcggcgac cgggtgccgg acgaggcgaa gccctggttc
    19261 acctcgctcg cggccgagag tgaccaggcg ggcgtcgaga acgagccgct ggatgccgag
    19321 gaggacgtcg agcgcggctg acggcacaat ggcgccgtga cccagctgga ccgtccgcgg
    19381 ccgtcgacgg cggccgtcat cgtcgcgctg ctcgtggtgt acgtggtgtg gggatccacc
    19441 tacctcggca tcgcgatcat gatcgagacg atgccgccac tcctggcggc cggcgtccgc
    19501 tacggcgttg ccggcgtgct catgctcggc gcgctcgtgg cgctcgctcg gctgcggccg
    19561 gccgccgcac cattggagcg tccgacgggt gcgcagtggc ggtcgacctt cgtggtgggg
    19621 acgctgctgc tgctcggcgg caatggtggg gtggtcctgg ccgagcagtt cattccctcg
    19681 ggcatcgccg ccgtgctcgt cgccatggtg cccatctggc tcgcggtcct cgacgcggtg
    19741 gtgacccggc ggcgtccgag ccgcctcgtg gtgggcggcc tggtcgccgg gctcgtcggc
    19801 gtggtcgtgc tactcgcgcc ggtcgagggg ctcggcggca tcgatccgtt gggcatcctg
    19861 ctggtcgtgg gggccagcct gtcgtgggcc atcggctcgc tgtacgcccg gtccgccccg
    19921 atgccgcgat ccggcctgct cggcacgggc atggagatgc tggccggcgg tgcggtgctg
    19981 gtcgtggcgg gctcgctgct cggcgacatc ggccgcgtcg atcccgggtc catctccacc
    20041 gcctcgctgc tggccatgat ctacctcatc gtcttcggtt cgatcctggc cttcaccgcg
    20101 tacacctggc tcgtcgccaa cgtcccggtg acgacgctcg gcacgtacgc gtatgtcaac
    20161 cccgtggtcg cggtcgctct cggggcgatc ttcctctccg agccgatcac cctgcgcacc
    20221 gtcctcgccg ccgtcctgat cctcggcgcg gtcatcgcca tggtgagcgg acgcccgcgt
    20281 gaggccgagg agccgggccc ggtgcccgag gcggccacac tggagcccga tgcgacgtga
    20341 gccgggacgc ggtccacgtg cggtgccagg cgacggatga cggctgggcc tgcaccgtgc
    20401 gggtcggcga cgatgcggcc gcgaccgagc acgccgtcac ggtccggcgc gacgatctgg
    20461 aggagctcgt ctccgggacg acaccggagg acgtggtcga ggcctcgttc cggttcctgc
    20521 tcgagcgcga gtcgcgcgag tcgatcctgc gccgcttcga cctgccggtg atcggacgct
    20581 actttcccga gtaccgggaa gagatccgcc gccggctgag ctgaccgccg gctcagggcg
    20641 ccggaggctg cggatgcatg gtccgcgggc ggccggcgcg gacgcgaccg aaggcctgcg
    20701 gccacacgac caggttccac gcatcggggt cggcggcgga cggcgcgatg agcccgtccg
    20761 cgccaagcga gcgcacccgg gcgccgagtc gctgcgccgc gtcccgcggc cccaccaggt
    20821 cgccgaggtc caccccgagc gcgtcgcgaa cctcgggccg ccgcaggtcc acgacccgca
    20881 ggtcctcggc acggacgctc cacagccgcc gacgctcctg gcgcgggtcg atggcgccgc
    20941 gcgtcgccgc cgaccactcc gcccagacgg tggcgggttc gagcgacgcg tacagcggcc
    21001 acggttcgcc cgggcggtgg taccggccct cgccgaccgc cgggcggtcg ggtgggaagg
    21061 ccggcgtgtc gacgccggtc tgacggtagg cgcgtcccga gaaggtggtg aggagctcgg
    21121 gcccggccgt cagacgtagt ctccgaccag gtacgactcg gcggcgcctc gcacggcgtc
    21181 gaagtcacct tccgccagcc ggtccaccgg ccgctgccac gccagttgtg cgttcggcgc
    21241 ctcgagccac gcgcgcttgg cggtgtcggt catgccacgg ccgagcagct ccaggatcgc
    21301 ctgcagctcg ccgatgcggt cgcggtagcg cgcctgcggc gcctgcccgg cgcgccagcg
    21361 ctccgcagtg cgcacgttcg cgccggtggc gcgcgcgatg aacctggcgg ggaagaggcg
    21421 gatgagcctg ggcggctccg cggcccgaat ggtcatggac cgatgctagc cagcggcggg
    21481 atgccggtca atccgacggt ggagatgccg gtgtgcggca gccgagggct agggagcggc
    21541 gccgcagcac aggcccgatg cctcgtagat gcggttcagc acgtagaggc catcggtctc
    21601 ggggtcggtg aggttggtct gtgcctgctc caacatgatg tacgggtgca tccgggcctg
    21661 gaccaggtcc gtcccgcgga acgacagctc gagcgtcacg ccctcctggg tctgctggct
    21721 ccactcctgg ccgaacagga agttgccgtg gctgaccatg gtcacgtggt agcggccgtc
    21781 ggcatcgggc gtgacctcga tcggtccgga ccagtgcgtg ccgtgcccga ccacctggtc
    21841 gcatccggcg tcgaagaaga cctcctgctg gcggcgcatg tcgccccgga actcggcgtg
    21901 gtactcggca ccgccccacc actgcggaac gcagaccacg acgtccgctc cctggctgcg
    21961 ggcgcgaccg gcggcgtcga cgacgttctc gtcgcgcagc caggcgacgc ccggggagtc
    22021 ggccgtggcc tccgcgaact cgggtgggat ctcgttgaag ccgacgaagc cgaaggtcag
    22081 tccgttgacg tccaccagcg cgggctccag cgcgtcgtcg aggttggtgc cggccccggc
    22141 ggtcgggatg ccggccgcct cgaacttgtc gagcgtctcc aggaagccgc tcacgccctg
    22201 gtcgaacagg tggttggcgg ccagcgtggc cacgtccacg ccatacgtgt cccgcagccg
    22261 cggcagcacg gccggatcga tgctgaacac cacgccgtcg ttgacgagcc agttgtcgac
    22321 gaccgggcac tcgaagtcgt cgagggtgat gtcggcgccg ctgaccagct cggcgacggc
    22381 cccctcgttc ccggtgcgga cggcgtcgac gatgttgaag ccgttgccga cgtagccggc
    22441 aggcaccggg ttggggtaga cggcggtgta ctgcgcggtc ccgccgccga agacccagtc
    22501 ccagccgcgg cccagggtga tggccgcgta cgccacgccg cggtcggggc aggagtcccc
    22561 gaggctcatc agggtgcgaa tctcgtccgg gtcgtacggg tcggcgccga cggccgtgcc
    22621 ggtgatcggg tagtcggccg cgcgcgcatc aggaccgccg aagatgtccg cgccgtccac
    22681 cgggagcacc ttcacgtccg gcgtgaccat cgacggcggc aggagggcca gcagcggctg
    22741 gtcctgcggg gcggtgttca gccgatcagc gatctcctcc gcctcgagac agtcgccgcc
    22801 agcggccacg gtgcggtcca ggtacgaccg gacgccgcag ggcacgagga gggtgtccgc
    22861 ggcggcctgc acctcgtcga gggtgatggc gcccgtcgac gcgatgtaac cggtgaccac
    22921 ggccagcggc acctccacct cgggatcggg cgtcggtgac gggctgggcg tgggggacgg
    22981 cgtcgcggac ggcgatgctc cgctggatgg gcgaaccggg gtgggttccg ccggggtggt
    23041 ctcggagggt ggagccagcg tgcaggcggc gaccagcgtc atgagcgtcg acagcacgcc
    23101 cgcgcgaaac gccccccgtc gcgtcactcg tcggtcccgc aggtgaggcc gccttcgacg
    23161 gcccgcgcca tctcgcggtc catggtctga ctggcgatgt agggcagggc cgccatggca
    23221 tcggccgtgt cgacggcggt cagatcctcg ttgatgctga gcagctccgc gcggatcagg
    23281 tgcagcgtgc ccatgatctc gagctgcagc cgccggccgg gaccccaggt cggaacctca
    23341 tcgagtgcgt tgacgatgtc ccgcagctcg tcggccaggg cgctcagctc atcctcgtac
    23401 ccggtcatgt cgccgccctc gtcgcccgcg gcgcgaatgg ccgcgagcct gggatcgagg
    23461 ttgacgatgc ggtcgaacag cgggcagatg gcggtgccgt acgcggctgc ggccgccggg
    23521 tcggcagatg ccgaggcgct gggcgcggga ggcgacgcat cggtcccccg ggcggtgggg
    23581 ctgacgggtg cggtggtgcc gcaggcgacg aggatggtcg tgagcgcggc ggcgacgagc
    23641 gccgcggagc gagggaagct gcgcatgggg aaccgcagta tagggccgtc cgtcacgaag
    23701 ccggcgcgag ccggtcccgg catccgccgg acgtgcggcc cggctcacgg ccgcggatct
    23761 gactacaatc gatgcgatcc catgaccgag cagctgcctc cccccgacgc cctcgccttt
    23821 ggctccggca cgtccgacga tcaccgcgac ggcattcgct gggtcgacta ccgcaacatc
    23881 agctggaacc ccgtcttctg caagcggtgc gacatctgcg tcgacatctg cccgaagggc
    23941 acgctggtgc tgcgcaacga cgcgatcatc gaagaggaca actgcatcct ctgcgggctg
    24001 tgcgagcgct actgcccgga ccttgccatc gagatgatcc cggaggcggt cgccgcacac
    24061 gccgccaaag agaagtgagc cggcgagtca tccgctatcc gacgggatgg acgctggcgg
    24121 tgctccccga tgccgcggcg gccgacgccg tggccggcga gctctccgcg ctgggtgttg
    24181 ccgccgacga cctcgtcgtc ctggccgggg ccgatgccgc ctcgcggctc gagcggctgg
    24241 gcacctccgc cggcgttgcc gccagggtcc gtcgcaccgt ccagttcatg accatggacc
    24301 agctgccgga cctgcacgtc tacgagctgg ccctggcgcg cgagcatccg gtcatcgccg
    24361 tccgggtctc cgacgccgat gctcggcgcc gtgcggtgga ggccctgcgc ggtcacggcg
    24421 gccacttcat caaccggttc ggcgcctggg cgacggagga gatcgcgcca tggcgcggcg
    24481 agcctctgcc cgtcggccag ctcatgcagc ggtgatggca tgactcgcaa gctcacgcag
    24541 gggaacgagg ccgtgttccg gggggccgtg gccgccgggg cgaactacta cgccggctac
    24601 ccgatcagcc cctcgacgga gatcctcaac gtcgcgtccg gctgggccgc cgagcatccg
    24661 gagttccgct tcctgcaggc cgaggatgag atcgcctcgg ccaacgccat catcggcgcc
    24721 tcgcttgccg gcgcgaagtc cttcaccgcc acctccggcc cgggcttcag cctcatgcag
    24781 gaggcggtcg gctacgcgca gaaggtcggc gtcccgtgcg tcttcgtcga cgtccagcgc
    24841 gtggggccgg caaccggcat gccgaccatg ccggggcagg gcgacatcat gcaggtcaag
    24901 tgggggagca ccggcgacta ccacccgctg gccttctatc ccaacagcgt gcccgaggcc
    24961 ttccgcatga ccgtcgaggc cttcaacgcg gcggaggagt cccgctcgcc ggtcgtcgtc
    25021 ctgaccgata ccttcgtcgc gcacctcaac gaggtggtcg acctcgacga gatcggtcgc
    25081 gaggtgcagg tcgtgccgcg cgacatcccg gcgctcggcg acttcgtgga cgagccgcgc
    25141 tttttcgccg gcgtcctgat gtacgagcac ggtcccaatg ccggcgagcc cgccacggcc
    25201 gatgcgaacg agtacctgcg ccagtactac gcctccaagc accgtcatct cgaggtggcc
    25261 ggccgctacg cctactacga gcacggctgg aacgtcgacg ccgaggtgct ggtcatctgc
    25321 tacgggatcg tgtcgcgggt ggcggccccg ctgcgcgacg agttcgcgtt gttccgcccc
    25381 atccgcatcc atccgatcct gggcgacgag ctgcgcgaca tcaccgcccg ctaccgcgac
    25441 gtcgtggtgg tcgaggccaa cgacggccag tacgcggacc tcgtcgagct cgcgatccac
    25501 cgccaggtga agcgcgtccc gctcctcggc ggccggatct cgctggaggc ggtacgcgac
    25561 gggatcgatc gcgtgctctc gggcggcccc tcggagccgc ccatcccgcc ggagccgacg
    25621 gaaccgcagc cgcgctcacc gctgggggtg ggatgatgcg ggcgccccga cgtacctgcg
    25681 tagctaccct gagggtcgag gcacgtaccg atgcctgaag cacccatggc cgagctcggc
    25741 tacaaggacc gcctgaaggt cgagctcttc ccgacgatct ggtgcccggg ctgcggcatc
    25801 ggcacgatca tgatgcagct ggccctgacg ctggacgaga tggggctcaa cgagcgcaac
    25861 accatcatcg tcaccggaat cggctgcacc gggcggatgg gcggctacct gaagcacgag
    25921 gccgtgtaca ccctccacgg ccggacgctc cccgtggcgg aggcgatcaa gacggtccgc
    25981 cccgagatga acgtcatcgt cgtggccggc gacggcgaca ccgcatccat tggcgggaac
    26041 cacctgatcc actcgatccg ccgcaacgcg ccggtcacgg tgctgtgcaa caacaacgag
    26101 atctacgggc tgaccggcgg ccagacgggc cccaccacgc cgaccgggac gaagacgctg
    26161 tcgagccccg ccggcaatcc gaacacgccg atcaacctgc agggcctggt ccgcagctcg
    26221 ccgcacgccc tgtacgcgaa gtcgacggtc taccaccagc tccacctgcg gaatgccatc
    26281 cgcgaggcga tcgagtggcc gggcttcagc ttcgtggaca tcacgagcca gtgcatcgag
    26341 aacaacggcc ggcgcatcgg cttcgccagc gccaacgaga tgctcacctt ctaccgcaag
    26401 acctacaagc gcgcgccgaa ggatgcggac cacctggcgc cgttcgagat cgggatcgtg
    26461 tatcccgcgc agcatcccgc ccgtaacggc aacgggacgg caccggcggc cgagaccgat
    26521 gggcagcgcc cggaggcgct gccggcgcag ccgaccggac cgatggcatc ggccaccgat
    26581 tcgccgggtg agggtggcgc gacgacgccc gaggcggcca cgaaggtgat cgaggcccgg
    26641 accgacgacg agaaggcgcg acgccgcgcg gagtcggtcg aacgggcggc gctcatggcc
    26701 cggctgtcgc ccgacgtcaa ggtgcgggtc gcgaagcgcg agatcaccct ggtgcaggcg
    26761 ctctcggagg cggggatcga gatccccgag tgggcgcgca ccggcgccag cgcgcccgat
    26821 gcccccgtcc cggccgggac ggtggagcgc tccgcgacgg gcgccgaccc ggcgacggag
    26881 gtgaagcccg agctcgacgc ccagaaggcg gaggcggcgg ccgagggccg cgacctgatc
    26941 ggcggtcagc ccggtggcga cacccagccc gcggcgccgc ggacgcccga agacgcggcg
    27001 cccgcatcgg tccaggcgcc agccgattcc agggaggcca agcgcgcgga gtcacagcgc
    27061 aagctcgcgc tcatgcagcg gctgccctcg gacctcaagc tccgggtcgc gaagcgcgag
    27121 atcaccctgg aggacgcaat gcacgaggcg gggatcgagc ccgaatgacc accaagatcg
    27181 cgctcgacgg ggtcggcggc cagggggtcc gggtcatcgc gggcgtcatg ggtgcgctcc
    27241 tcgcccgcat gggcaaggag gtcacggtcc tcttcgacta cgactcgagc gtccggggca
    27301 gcatgtcgga cgccttcctg atcttcgacg atcacccgat cgagaacccg gtggtcgagg
    27361 aggcggacat catgctgaag ctggccgaca aggggcacct gcgcatccac ggccggacgg
    27421 tcgtgacgga cctcggcctc cacgtccccg gcgcggagca gatccccttc gcctcgctgg
    27481 gcttccagca cttcggcaag gagctgttcg gcaacatgat cgcgctggga cggctgctgc
    27541 ggctggcgaa catcgagttc gacgagccgg caatccgcga gtccctgcca cgtcgctacc
    27601 aggacgagaa cgtggcggcg atccgcttcg gctacgagct cacgccggag tcgatcgccg
    27661 cctacgccgc cgcggccccg ccgtcgcggt tccagatgaa ggacgcggcc accgcgccgg
    27721 gcgggcatcc ccgcggcgcg agcgtcggga tcgggacggc cgccgacgtc ggcgagggcg
    27781 gctgacgccg gcacacctcg accgccgcat cggtcaaccg ccgccgccct cgatcggctg
    27841 ctgctcctcg ctcggcccga caccctcgtc gttgagcggc gcgccgccgt ccgtcgctgg
    27901 ctcggtgacc gtcagctgca cctcgaggcc ggcgtcccgg tcgccgtcca ccgggctgta
    27961 cacggtgtag gtcccgggct ggagctccac ctggagcgtg tcgagctggt cgggacgcag
    28021 ctcgtcgagc gaagcctcga ccccttcgat ggcgaagccg tgcgacgagg cgccggcgtt
    28081 cgagacctcg aactcgaccg ccccgcccgc gatctcgctg ggcatctcga tggcatcgtc
    28141 ggtcaggcgt acctcgaccg cgatggcgcc agggctgggg ttggtgccct cgttggtgtt
    28201 ctcgtcccgc tcgttgaagc agcccgccag ggccacggcc atgaccagca ggatggcgcc
    28261 ggccgcggcc ggacgtgtcg tgatccagtg catggcgtcg ccctaccgca ggaccgctgc
    28321 caatgccctg ctgcgtccgt gccgtcgagc gttgcgaccc gccgcgccgc cggagtaccg
    28381 ttcggcccat gagctccgac cccgtcgagt ccaatccggg cctgtaccgc gtcgtgatgg
    28441 agaacgagcg cgtccgcgtg ctcgagtacc gcgatcgccc gggtgaccgg accacgccgc
    28501 atggccatcc cgacagcgtg atggtcaccc tctcgagctt ccgtcgccgg ctggtgagcg
    28561 gggaccagca ggccgaggtc gagatcccgg ccggcgtggt gcgctggctg gcggcgcagg
    28621 agcacctggg tgagaacatc ggcgactcgg cgacccacgt ggtcttcgtg gagctcaagg
    28681 agcctcggcc gcagggctcg acggcgaccg caccgggcgc gctcggtcca tcggagggct
    28741 gaggaccgat ggagcgcggc gccgatgccc ttgatctcga cgtcatccgc cgcgcgccga
    28801 aggtgctgct gcacgaccac ctggacggtg ggctgcggcc gcgcacggtc atcgagctgg
    28861 cgcaggcggc cggctaccgg gacctgccga cgacggaccc cgacgagctc gcgcgctggt
    28921 tcaccctcgg cgccgaccgc aagtcgctcg agctgtacct ggagggcttc cgccataccg
    28981 tggcggtgat gcagacgcgc gaggccatcg agcgggtcgc cttcgagtgc gtcgaggacc
    29041 tggccgccga tggcgtcgtc tacgccgagg tccgcatggc gcccgagctg ctgaccgatg
    29101 gcggcctgtc gctggacgag gcgaccgacg ccatgctggc cggcttccgg cgcggcatgg
    29161 agggccggcc catcaccatc ggcctgatca tcacggccat gcgccaggcg gcgcggtccg
    29221 tggagatcgc ggagctggcg gtgcgccacc gcgacgaggg ggtcgtcggg ttcgacatcg
    29281 cgggccccga ggccgggtac ccgccgactc gtcacctgga cgcgttccac ctcatcgctc
    29341 gcgagaactt ccacttcacc atccacgccg gcgagggctt cggcctcccg tccatctggg
    29401 aggcgctgca gtggtgcggc gcggagcgcc tggggcatgg ggtccgaatc gtcgacgaca
    29461 tcgccgtccg gcccgacggc cgcgtggccc tggggcggct ggccgcctat gtccgcgacc
    29521 gccgcatccc cctggagatg tgcccgacct cgaacgtcca caccggtgcc gcgccgtcga
    29581 tcgaggagca tccgatcgac ctgctccgcc gcctgcggtt ccgcgtgacc gtgaacaccg
    29641 acaaccggct gatgagcggc atcacgctca gctccgagtt cgcgaccctg gcgcggacgt
    29701 tcgggatcgg cctggacgag atggagtggc tgacgctgaa cgcgatgaag tcggcgttct
    29761 ggcccttcga cggccgcctc aagatcatca acgcccagat caagccgggc tacgcgacgc
    29821 tccgcgctca acaggccgcc gcggagctcg ccacgacctg acagcccggc ggcctagacc
    29881 gatgcgggcg ccaggaactc cgccaccgca ccctcgagcg cggctgcctc ggactcgttg
    29941 aggggtcgca ggggcgcgcg catcccctcg gtcacggcga ggccccgcat gccgagcacg
    30001 tgcttcgcgg ctgcgatgaa ctggccgctc gcctccatgg ccccccgaag ctggcgcaac
    30061 cgagccatgg cggcctcgtc gccggcgtcg agggccgccc ggaccacgtc gggaaacgcg
    30121 gccgacatgc cggatacggt gccgagggca ccgcgcgcca gtcccgcccc gatcagcggc
    30181 tcgctgccga tcatcaccgg cagcccgagg tccaggtacg gctccacgcg ctcgaacggc
    30241 gcctcggaca ccttcagccc cgccaggttg tcgaccgatc cgccgatgcg ccggacgacc
    30301 tcgaccggca gcggatagcc ggaacgcgcg gtgaaggcgt agcagtagaa gggcaggggg
    30361 gcggccgcgc gggcggcggc gacgaagtgg gcggtcagcg catcggcgtc gagggcgaag
    30421 tagggcggcg ggatcaccgc gaccgcatcg gcgccaatct cccgggcgtg ctcggcgagc
    30481 gcgaccgtat ccgcggtggt ctgggcgccg gcgtggacga tcaggatgcc ggagagcgcc
    30541 gcgcggaagg cctcgacggc gcgccgtcgc tcatccatgg tcagcagcac gccctcgccg
    30601 gtcgtcccgc acgcgaagat cccgtcggcg ccgcgggact cgaggaaggc cacgtacggc
    30661 gggatggcgt cggcatcgag ggcgcttccg tcctcgctga gcggcgtgac ggccgccacg
    30721 acaaggggcc gatgatcaag gtcggtgagc tgcatgcggc ccatcgtacc gaccgcaggc
    30781 gcggtgttca gcgaagaccc ccacgccgtc gaggggggcc caggtcgtgg cggggaggcg
    30841 cccgctcaac ggacgtggag atcggcggcg tcctgggtca ttcgggtcat gcccatcggc
    30901 ggcgcggccg accgccgggc ggcatcggtg tagactcggc cggagtcgaa cggccacgcc
    30961 agcccctatc ggcgactgcg aagtgcattc gaggaggcca tgacctacgt catcaccgag
    31021 ccctgcatcg gcgtcaagga cgcgagctgc gtcgacgtct gtccggtcga ctgcatccac
    31081 gccaccgacg acgacccgca gtactacatc gatcccgacg agtgcatcga ctgcggggcg
    31141 tgcgagccgg agtgcccggt gaccgcgatc ttcgccgagg acgccgtccc cgacgaccag
    31201 gtgcagtaca tccagatcaa cgcggactac ttcaagcgct gatgccgcgg ctgatcgcgt
    31261 tgtaccggcc gcccgaggat cccgcggcgt tcgacgcgca ctaccgcgac gtgcacgacc
    31321 cgatcgtgcg gcgctacccg aagctggccg gcgtgcggat cacgaagctc gccggcgtcg
    31381 ccggacggcc agcgccgtac taccagatgg ccgagatgat cttcgagacc gatgcggacc
    31441 tcgccgaggc gctcggctcc gagcccggca tggagagcgc gcgcgacctg cgcaacttcg
    31501 cgaccgccgg cgtcgagctg ttcatcgcac ccgacgaggc cgatggctga gcccggctca
    31561 ccggtcccga ccgatccgcg cgagctccgc tacgagcgca tctcgtacga gcgcgacacg
    31621 gagaccggga tcgcgaccgt caccatccag cggccggagg tcctgaacgc cctcgacctg
    31681 ccgacgctga acgagatggc gcacgccttc cagcaggcct cgtgggatga ccgcgtcgcc
    31741 gtggtcgtcg tcaccggggc gggcgatcgt gccttctgca ccggcgcgga tctcgacgag
    31801 caggcgctga tgggctcgac cagcggccag tactggcgct ggatgggcgc cttcatcgag
    31861 atgcacgacc ggctgcggca gatcggcaag ccgaccatcg cgcggatcaa cggcgtgtgc
    31921 gtcggcggcg gccaggagct gcagctggcc tgcgatctct cggtcatggt cgacgacgcc
    31981 tacatccgcc acgtcgggcc ggagcatggc tccgtgccgg ccggcggggc gacgcagtgg
    32041 ctgccgctgg tcgttggcga tcgccgcgcg cgcgagatcg tgatgctgtg cgagccggtg
    32101 tcgccgcagc aggcgctcga ctggggcctc gtctcgcggg tggtcccgcg cgaccagctg
    32161 gacgcgaccg tccaggagtt ggggatcaag ctggcgcgca agctcccgga ggcgctgcgc
    32221 tacaccaaga cccagctcaa ctggtggcgg gacctggtgt ggtcgcagac gatccagcac
    32281 gcccgcgatt ggctcgccgt tcactccacc gcggatgaga ccaaggaggc ggttgacgcg
    32341 ttccacgaga agcgcaagcc gcggttcgcg gagctccggc gcctggaggc cggggaggga
    32401 cgcgtgtgtc ccgcatgtgg tgcgaccggg ctgccggcgg cgcatcggtt ctgcggggcg
    32461 tgcggcgcgg agcttccgga cgcgtggggt gccgcgacat gacacaaccg acggcccccg
    32521 aggagctcgt gctcgtcgag cgacagcccg ggcagcgcac ggccctggtc cgcctcaacc
    32581 gcccgaagca gctcaacgcg ctgaacggcg ccgtcatgga tgcgctgtgc gtcgcgctcg
    32641 aggagctcga ccgggacgac gaagtccgcg cgatcgtggt gaccggcagc gagcgtgcct
    32701 tcgcggcggg ggccgacatc ggcgagatgg ccaacgccac ccccatcgac atgctgcgca
    32761 cgaaccgcat cggccagtgg gaccgcatcc ggaggatcac caagccgctg atcgcggcgg
    32821 tcaacggctg ggccctgggc ggcggctgcg agctggcgat gaccctcgac ct
//
LOCUS       DASTYP010000038         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_402716, whole genome shotgun sequence.
ACCESSION   DASTYP010000038 DASTYP010000000
VERSION     DASTYP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402716"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /gene="rpsC"
                     /locus_tag="VFV59_01880"
     CDS             complement(<1..919)
                     /gene="rpsC"
                     /locus_tag="VFV59_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU5324606.1"
                     /translation="MAHKVHPYGFRIGVNKPWLAKWYADRDYATLLQEDMRIRELVAK
                     QLANASVSQVEIERGINHVTVTVHTAKPGIVIGKGGANVEALRTAIGKLTNKKVKLEI
                     KEILQPELDGMLVAQNVAGQLERRIAFRKAIKQSIQRSIKAGAKGVKIQVSGRLGGAE
                     MSRTEWDREGRIPLGTLRADISYGVVLARTTYGAIGVKAWVYRGDQLGERPGSARTEP
                     RPARRGAPAAGGRGRTGGPRPTDGAPRPPRAPRAAVVDQEQRASAPAPVDATPTTDAA
                     AAAPAAPEPITTTASMTESGAASVEATQAP"
     gene            complement(922..1263)
                     /gene="rplV"
                     /locus_tag="VFV59_01885"
     CDS             complement(922..1263)
                     /gene="rplV"
                     /locus_tag="VFV59_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU5324607.1"
                     /translation="MRVTATAKYIRVSPRKVRLITNLIAGKPVEEAAAILRFLPNAAA
                     KDVAKVLKSATANAENNYNLSADELRVVSAVADEGPTLKRGRPRAQGRYFQVLKRTSH
                     ITVAVADREEP"
     gene            complement(1273..1560)
                     /gene="rpsS"
                     /locus_tag="VFV59_01890"
     CDS             complement(1273..1560)
                     /gene="rpsS"
                     /locus_tag="VFV59_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5324608.1"
                     /translation="MSRSVKKGPFVDARLLGRIDDMNKRNERKVLKTWSRASVIFPQM
                     IGHTIAVHDGRRHVPVFVTENMVGHRLGEFAPTRTYRGHTGRATQRSTALK"
     gene            complement(1571..>2165)
                     /gene="rplB"
                     /locus_tag="VFV59_01895"
     CDS             complement(1571..>2165)
                     /gene="rplB"
                     /locus_tag="VFV59_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5324609.1"
                     /translation="VEYDPNRSARIGLLHYADGDKRYMLLPHGLGVGDVVASGPDVEA
                     RNGNALPLSRVPLGTQVHNIELVAGRGGQIVRSAGSSAQLLAKEGDFATLRLPSGEVR
                     RVRIECMATVGQVSNLDHENQKIGKAGRARHMGKRPTVRGSAMNPRDHPHGGGEGKQP
                     TGMPPKTKWGKPAMGLRTRAKKNQSSKLIVRRRYGRG"
BASE COUNT          256 a          758 c          763 g          388 t
ORIGIN      
        1 ggggtgcctg ggtcgcctcc accgaggcgg cgccggactc ggtcatcgac gcggtggtcg
       61 tgatcggctc gggtgcggcc ggcgcggctg cggccgcatc ggtcgtcggg gtcgcgtcga
      121 ccggggcggg tgcgctcgcg cgctgctcct ggtccacgac tgccgcccgc ggggctcgcg
      181 gcggacgcgg cgcgccgtcg gtcgggcgag ggccaccggt tcgaccgcgg ccaccggccg
      241 ccggagcgcc acggcgcgcc ggacgcggct cggtgcgcgc gctgcccggc cgctcgccga
      301 gctggtcgcc gcggtacacc cacgccttga ccccgatggc gccgtacgtc gtacgggcca
      361 ggaccacgcc gtagctgatg tcggcgcgaa gcgtgcccaa cgggatccgc ccctcgcggt
      421 cccactcggt tcggctcatc tcggcgccgc cgaggcgacc cgagacctgg atcttgacgc
      481 ccttggcgcc cgccttgatc gagcgctgga tggactgctt gatcgccttg cggaaggcga
      541 tgcgccgctc cagctgaccc gcgacgttct gcgcgaccag catgccgtcg agctcgggct
      601 gcaggatctc cttgatctcg agcttgacct tcttgttcgt cagcttgccg atggcggtgc
      661 gcagcgcctc gacgttcgca ccgcccttgc cgatgacaat gcccggcttg gccgtgtgga
      721 ccgtgacggt cacgtggttg atgccgcgct cgatctcgac ctggctgacg ctcgcgttcg
      781 cgagctgctt cgcgaccagc tcccggatcc gcatgtcctc ctggagcagg gtcgcgtagt
      841 cgcggtccgc gtaccacttc gccagccagg gcttgttgac accgatccgg aagccgtagg
      901 gatggacctt gtgcgccatg cttacggctc ctctcggtca gccacggcga cggtgatgtg
      961 gctggttcgc ttcaacacct ggaagtaccg gccctgggcg cgcggtcgcc cgcgcttgag
     1021 ggtcgggccc tcgtcggcga ccgcgctcac cacgcgcagc tcatcggccg acaggttgta
     1081 gttgttctcg gcattggccg tggcgctctt gagcaccttg gccacgtcct tcgccgccgc
     1141 gttgggcagg aagcgaagga tcgccgcggc ctcctcgacc ggcttgccgg cgatgaggtt
     1201 cgtgatcagc cgcaccttgc gggggctgac gcggatgtac ttggcggtcg cggtcactcg
     1261 catcggtcgc gcctacttca gcgcggtcga gcgctgggtg gcccgaccgg tatgcccgcg
     1321 gtacgtgcgc gtcggggcga actcgccgag ccggtgcccg accatgttct cggtgacgaa
     1381 gaccggcacg tggcgacggc cgtcgtggac ggcgatcgtg tgcccgatca tctgcgggaa
     1441 gatgacgctg gcgcgagacc aggtcttcag caccttccgc tcgttgcgct tgttcatgtc
     1501 atcgatgcgg ccgagcaggc gcgcgtcgac gaatggtccc ttcttgacgg aacgactcat
     1561 ctatcggctc ctaaccccgt ccgtaccgac ggcggacgat cagcttgctg ctctggttct
     1621 tcttggcgcg ggtacgcagg cccattgccg gcttgcccca cttggtcttc gggggcatgc
     1681 cggtcggctg cttgccctcg ccaccaccgt gcgggtggtc gcgcgggttc atcgccgatc
     1741 cgcgcaccgt cgggcgcttg cccatgtgcc gggcacgtcc cgccttgccg atcttctggt
     1801 tctcgtggtc caggttgctc acctggccga cggtggccat gcactcgatg cgcacccggc
     1861 gcacctcgcc cgacggcagc ctgagggtgg cgaagtcgcc ttccttggcg agcagctggg
     1921 cgctgctgcc ggcactgcgg acgatctgac cgccgcggcc ggcgaccagc tcgatgttgt
     1981 ggacctgcgt gccgagcggc acccgggaca gcggcagggc gttcccgttg cgcgcctcga
     2041 cgtcggggcc ggaggcgacc acgtcaccaa cgcccagacc gtgcggcagg agcatgtagc
     2101 gcttgtcgcc atcggcgtag tggaggaggc cgatgcgcgc cgagcgattc gggtcgtact
     2161 cgacc
//
LOCUS       DASTYP010000039        36981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1386535, whole genome shotgun sequence.
ACCESSION   DASTYP010000039 DASTYP010000000
VERSION     DASTYP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 36981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36981
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386535"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFV59_01900"
     CDS             <1..306
                     /locus_tag="VFV59_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5324610.1"
                     /translation="MYAVGDATDLPLSKAGSTAHFEAPIVVERIVAGVEGREPDAVAG
                     NYTGKVMCFFEVGDGKGTLLQFDYEHPPRPPKPNRLWHLGKIVFNKTYWHTVPKGRV"
     gene            605..1876
                     /locus_tag="VFV59_01905"
     CDS             605..1876
                     /locus_tag="VFV59_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5324611.1"
                     /translation="MRMVSAAEAVTGIQSGDQIFVQGGAATPTPLIQALVARAEELRD
                     VGIIHVHTEGPAPHLAPEMAPHFRHRALFIGANAREAVNDGRADYVPVFLSDIPELFT
                     NGVLPLDWVFLNVTPPDAHGFCSLGTSVDCTLAAARAGARVVAQVNPAIPRTLGDSFI
                     HVDEIDLGVSVDRPPYEHHLDPIGDVERRIGDYVAELVPDGATLQMGIGAIPTAVGLA
                     LRHKKDLGVHTELFTDPVLDLVEAGAITGAAKEINRYKIVTAFLMGSRRLYEWIDDNP
                     MVEMRPVDYTNDTAVIRRFRRMVAVNSAISIDLTGQVSADSIGTRFYSGFGGQMDFMR
                     GAALAEEGRAIIALPATAAGGTVSRIVPTLTEGAGVVTSRAHVRTVVTEFGVAELFGK
                     SVRERAAALIAVAHPDFRDELCHEAKRRYHV"
     gene            1976..3952
                     /locus_tag="VFV59_01910"
     CDS             1976..3952
                     /locus_tag="VFV59_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5324612.1"
                     /translation="MSSKHAAPPAARFPGTPAVMDGSEAIADVETRIAEGACAYPITP
                     STNMAAAFQAAVADGRTNLWGTPLSFLELESEHSSATAAEGYALAGGRVTNFTAGQGL
                     VLMKEVLHVISGKRLPAVFHVGARALTSQALNIHAGHDDLMAVADTGWGMLIARNAQE
                     AADLTAIGRRVAEETETPFFVAQDGFLTTHTLENVQRPEDDLLRTFVGDPRERIRDYF
                     APSEALMTGVVQNQDAYMKGKIGQRAFYDRLHGALVSAMTEWTELTGRPYAPISAHRT
                     EDAEHIVVCMGTMADTAIAVVDRLRAAGRRVGCLTVTSFRPFPAEELGEAVRNASAVA
                     VIERTDEPAAHDNPLTRELKAALADVAMSGARIPYVVSASAGLGSRDVAAGDIAAVFD
                     WIADPEAVRQRRYAVLGIRHPLALERIPIDIRPDGAYSLRGHSIGGFGSVTTNKLLAT
                     LMGDLFGLHVQAYPRYGSEKKGLPTTYYLTIADGPIRQHGELDTVEFVPLYDVAAFRQ
                     GNPLTGLSDRGTVFVQTHLGDPEAIWGSVPVAARTQMLERGLRLWALDTAALAQRHAP
                     RPDLLVRMQGVALAGVFLRVSPFAERAGFDRETLLAALRTQLERFFGKRGRAVVDANL
                     AVIAEAYDSVIDVTDAVVATDQTLPAPRILEVVS"
     gene            3949..4359
                     /locus_tag="VFV59_01915"
     CDS             3949..4359
                     /locus_tag="VFV59_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5324613.1"
                     /translation="MTRSLTVGALMSTDPIVVPADAPLTQAAELLERHHIHGLPVVDA
                     FGGVVGVVSQTDMLRARTTDDLWSRWPGLKVRHLMSSPALTISADADVAEAANRMELE
                     RVHRLVVVGPDGRSPVGVLSTTDLVHGMLEEARA"
     gene            4356..6038
                     /locus_tag="VFV59_01920"
     CDS             4356..6038
                     /locus_tag="VFV59_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5324614.1"
                     /translation="MTLTTDPLLKEPREEKELHEARLATTAPLAFDVEAYVDDFNSVV
                     VGAYRAGSADAGLPADASVARSVIPPGTAAVRDFSTLAPQLPAFISERCVGCMACVNA
                     CPDSAILAVAQPGSVLEPAIAACVAETGDGAGASVEADLRSHFVTTTKYATVPAKKGL
                     EPAEFGIFVDPVHCKGCAECVEVCSALGHDALLMTDKLPVEEVSGTSTVDRYARDFRF
                     FRTLPVTPEPYRNEKALADVMLGEHAFGYVGGAGSCSGCAEATALRMLLAATRQVHGP
                     ESMGIVAATGCNTVFGSTYPFNPFLVPWTNSLFENGPADALGIRARWDQAGHADRRLW
                     VVGGDGAMYDIGFQSLSRLVASGADVKVMVLDTQVYSNTGGQASTASFGGQVTKLSAY
                     GKSLHGRPEQRKELARILMAHGEVYVAQTTPAHINHFYRSVMDANAYPGPAVVVVYTP
                     CMPEHGIADDAASRQAKLAVDSRAFPLFTYDPRRGPSMAERLSLQGNPALKEDWERLH
                     DGGATDFVAFARTEGRFAPHFAPDGTPSNELLATQADRLANWRLLQELAGIR"
     gene            complement(6176..6646)
                     /locus_tag="VFV59_01925"
     CDS             complement(6176..6646)
                     /locus_tag="VFV59_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324615.1"
                     /translation="MHPQTERLIEVAGDGFREAARMADAHRATIPISTTRHRVLVVDE
                     DVRVRTLLLRALLDRGHAVAAADGLARLTRSPYRRFRWCVAFVDVTGRARPDDDELRG
                     LVGSDAAVILLTADPAAADLTERRRDARLLKPLFVHDWLRLMDTRCRRHPQSVA"
     gene            6753..7535
                     /locus_tag="VFV59_01930"
     CDS             6753..7535
                     /locus_tag="VFV59_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5324616.1"
                     /translation="MHRPRERWEDVVEPDGKQPSDPVTGDPVVRGSAINGILARLPPR
                     ELAAFTVAARPFRAPKGQLLAAPGTTSAAIFPIRGLISLGLTDERGETMTVDVVGREG
                     MAGLWLYLDDVPTPYLVSALVPLAGWLIDAGALRRVAAPGTTLATLLGRYAQYRLALR
                     AHLMHCNRTHELTPRLARFLDGCARASGSAELELSHEDLASGVGAHRPAVTDALHRLA
                     ALGAVRSGRRLVEVSSPAGLQAAACTCAATIAELQERFDRPD"
     gene            7610..8092
                     /locus_tag="VFV59_01935"
     CDS             7610..8092
                     /locus_tag="VFV59_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5324617.1"
                     /translation="MNEHDFVAELGATWFGAGLSLHARARLGECARLLTHPANDVLLR
                     EGDDTPFFGILTRGRVGLRMRVPERGPVTILTVEPGDVVGWSALVPPHRATSTAVTIE
                     PSTLIEFEPRRLRELLEHDLALSATLYPRLLEAIGRRLSATRQQLLDLYAQQAEPVPW
                     "
     gene            8086..10032
                     /locus_tag="VFV59_01940"
     CDS             8086..10032
                     /locus_tag="VFV59_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5324618.1"
                     /translation="MVTDAGFLPRADLDRLIGLLREGGRTVIGPTLDDGALVYAEIGS
                     ADDLPTGWTDDAAPGRYRLVRDEGQRTFGLGPPPQGLKRFTFPSRAPLAVAERTAEGA
                     VTFTAAVPDPPRLAFLGVRPCEVAALLVQDRVLAGGPFVDEDYRARRQASLVVAVACT
                     RSGSTCFCASMGTGPEVTAGHDLAMTELDDGFVVSAGSRAGEQLLARLALPAVTPDQH
                     ATATAAVAGARAAQGRQVVTTGLRDRLMDQLDHPRWAEVAERCLACANCTMVCPTCFC
                     TSTALDSDLDGAATRMERRWDSCFTGDFARVAGGNFRPRRQDRYRQWLTHKFATWVDQ
                     FDTFGCVGCGRCITWCPVGIDVREELAAIAPPYEELPTATPVVAGSTSAYSTAVVRSV
                     TPETADTSTLALVPGDDALLAARPGQFVMVEQPGFPPLPISVSRVADGALWLTIRAAG
                     PATTATIALRPGAQLGLRGPLGRGWDIQRAVGRDVVIVAGGIGLAPVRPIIDTVLRDR
                     EKFRHVILAYGARTPADRLYVDEMADWADRGMVVTEIVDRAGPEWLGRVGIVTHLLDQ
                     RDFHDRPATAFVVGPERMMEATVATLAERGVPRERIFVSLERHMQCGIGLCGHCQMGP
                     FFICRDGPCFAVSELGDLFGREGI"
     gene            10032..10847
                     /locus_tag="VFV59_01945"
     CDS             10032..10847
                     /locus_tag="VFV59_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008939164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEU5324619.1"
                     /translation="MISDRPRVGVVKLASCDGCQLTLLDLEDELLPIVGRFDLIEFPE
                     ATSLRSAGPYDVLLVEGAVSTPEQAEEIVHLRARSRLLVSIGACATAGGIQALRNWTD
                     EPDWRASVYPEAGWIESLATATPVSEYVPVDAELRGCPIAPGQLLELLTALRIGRRPQ
                     LPAESVCLACKRRGNECVLVARGIPCLGPVTVAGCGALCPTFGRGCYACFGPREEANV
                     ASLHGWLAADRSTQEVDRLFAGFTAWSPPFREPAARAVAVAETHHPRKDDPDA"
     gene            10840..12174
                     /locus_tag="VFV59_01950"
     CDS             10840..12174
                     /locus_tag="VFV59_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEU5324620.1"
                     /translation="MHEVEAAPEAVTEVGDVTRFAVKQLTRVEGEGRLDLAVRDGVVT
                     QAHLEIFEAPRYFERIVVGRTPDEVIDIVARICGICPVAYQMSAVHAFEDLFGVAVDP
                     QIRALRRLFYCGEWIESHALHVYLLHAPDFLGYPSAIELAADHGPIVERGLRLKKVGN
                     DLVALLGGRPIHPISVRVGGFSRVPKAAELEPMRARLAGALVEAIETVRWVATLEPPP
                     YARVPRLVSLVHPTEYPMNDGRITSSDGIAVAPSAWTDAFEEQQVPWSNALQARARDG
                     VPYLLGPTARVVLGGSTLHPEAAAALAETGLGDELQNNVFRSIVARGVELVHAIAEAI
                     DIIDAYRRPVEPKVAWEARPGVAAWATEAPRGLLFHRYEVDEAGLVSRAQIVPPTSQN
                     QRAIEHDLATFAPSVLELPHEEATHRLEQLIRSYDPCISCATHFLELKVDER"
     gene            12174..12665
                     /locus_tag="VFV59_01955"
     CDS             12174..12665
                     /locus_tag="VFV59_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEU5324621.1"
                     /translation="MTVTEMRHVTILVCGEPMRGDDAVGEAVVRALPATTLQLGEVCH
                     VGGLMPDDLMAAGAPVIVVDAVHGLPAGTVVDVPLAALPELAAVGATPESTHAIPLPT
                     VLGIVERLAGELPEGRFIGVAAGTDLIGAPLSEPVREAVDVCAARLGHWIRTLAHPTV
                     GTS"
     gene            12665..12886
                     /locus_tag="VFV59_01960"
     CDS             12665..12886
                     /locus_tag="VFV59_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEU5324622.1"
                     /translation="MCLTAPVRVIDVSGTTATVDAAGRRRTASALAVPGIRPGDWALL
                     SAGTLVRVIDPALAAEIADAFRTATGEQP"
     gene            12883..13833
                     /locus_tag="VFV59_01965"
     CDS             12883..13833
                     /locus_tag="VFV59_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5324623.1"
                     /translation="MIERIRAADRGGHRLTIGLALAGMTALVSGVSVFLNATAVRSVG
                     DPVLFTTLKNGVAALVLVAVALALVPRPAATIAALSGRTKLGLLALGVVGGSIPFVLF
                     FTGLASASAPAAAVIHKTMFFWVALLAVVLLRERLGLLQVGALGVLLVSQLLIQPPDG
                     ISWTSGETMIAIATALWAVEVIVAKRLLRAVPSPIGAAARMGIGLVLLVAYLAWTGGL
                     AKLGGLGLEQWAWVLGTGLLLSAYVATWYAALRRAPATAVTAVLTVAAPITAALQLVA
                     NGQVPAAGATIGYGLTLVAGAVVAVMVLRRPAASREAALA"
     gene            13845..14615
                     /locus_tag="VFV59_01970"
     CDS             13845..14615
                     /locus_tag="VFV59_01970"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEU5324624.1"
                     /translation="MAATTIPAAAPDAVAGPILFARYAYPPNRLGLCGPDDAPGLLDG
                     ATAGADRQLRSLALGFEGAYPYLRLIAEANGIADPLDARVVEAYWLGSPLTSAVRPRP
                     LHRDLEDRFRGRMTASDWRWLEAAVGAASRPVHAFHVLEVFPRVGLLRGGPADPIVET
                     MDSCRIRPARVLAVDGGHLTVAARPLTLVDGQLQLGDERVESADAWRGSDGVLDGVQV
                     GDWVSLHWGWACDRLSRPQLARLVRWTGAAIRTANRSM"
     gene            14621..15736
                     /locus_tag="VFV59_01975"
     CDS             14621..15736
                     /locus_tag="VFV59_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5324625.1"
                     /translation="MTASSDIQRWHDNLQGEVDGSHVYRAMAANAGDDRLSELYERLA
                     AVEDRHAQLWQERLTDAGARAALRPTWRARVLAAVARRFGAGVVAPVIASQERQGRTM
                     YDTQPEAAGTSLPADERSHARLLRTVTGGAAGATLARFEGRHRAIGGNALRAAVLGAN
                     DGLVSNFSLVMGVAGASSGGEPVLIAGIAGLLAGSLSMALGEWLSVQSARELYANQIA
                     TEAEELRTFPAEEEEELRLIYEAKGLDPEEARSLAQRIIAGDPQVALDTMAREELGID
                     PEELGGSAWTAAFTSFGLFAAGAIIPVLPFFVTAGMTAIIASAVLSGVALFGIGALIT
                     ILTGQPALASGARQLGFGLAAAAITFAIGSLLGTALG"
     gene            15764..16057
                     /locus_tag="VFV59_01980"
     CDS             15764..16057
                     /locus_tag="VFV59_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEU5324626.1"
                     /translation="MDERRQEFSVNGDQLVAKVRELIEEGNVRRLIIKNEDGRTLLEV
                     PLTLGVIGAALLPVFAAIGALAALATRCTIVVERAEAAEVPPATTPESTTDLT"
     gene            16172..16675
                     /locus_tag="VFV59_01985"
     CDS             16172..16675
                     /locus_tag="VFV59_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU5324627.1"
                     /translation="MHVLVTAASKHESTAEIADAIAASLRAAGITVDRSPPDQIASVD
                     GYDAVVVGSGVYAGRWLDPARAFVERHTDALRRRPVWVFSSGPLGDPPLPADPSADGV
                     SLADRIGAREHRTFSGRLDPGRLGFLERTVVKAFRAPAGDSRDWDAIRTWGTEIAGLL
                     AATAKGG"
     gene            16949..19354
                     /locus_tag="VFV59_01990"
     CDS             16949..19354
                     /locus_tag="VFV59_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEU5324628.1"
                     /translation="MNARIAEPATVAPPKTDELRRIDAYWRAANYLSVGQIYLLDNPL
                     LREPLRPEHIKPRLLGHFGTVPGLNLVYVHLNRAIRQRDLNAMYVTGPGHGGPGLVAN
                     AYLEGTYSEVYPTISQDVDGMRMLFRQFSFPGGIPSHVAPETPGSIHEGGELGYALVH
                     AYGAAFDNPDLVVAAVVGDGEAETGPLAASWHSNKFVDPARDGAVLPILHLNGYKIAN
                     PTVLARIPEEELVKLLEGYGHSVEIVSGDDPMKVHRAFAAALDRALDAIADIQRRARS
                     GKGEAKRPRWPMIVLRTPKGWTGPEEVDGKPVENTWRSHQVPMADVRDNRKHLKLLED
                     WMRSYRPDELFDESGRLRPELAGLAPRGERRMSANPVTNGGAVLQGLRLPDFRDYAVE
                     VTEPGGVTSEATRVLGEFLRDVIDRNPTSFRLFGPDETASNRLQAVFEKTDKAWMAER
                     LPVDEHLAPDGRVMEVLSEHLCQGWLEGYLLTGRHGLFDCYEAFIHIIDSMFNQHAKW
                     LKVTRDIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKSEIIRVYLPPD
                     ANSLLSVADHCLRSRHYVNVIVAGKQPMPNWLSMDDAILHATRGIGIWTWASNDEGQE
                     PDVVMACAGDVPTLETLAAVDILRRHLPDLKVRVVNVVDLMRLQDPGEHPHGLSNREF
                     DALFTTDRPIVFAYHGYPWLIHRLTYRRTNHDNLHVRGYKEEGTTTTPFDMVMLNDLD
                     RYHLVMDVIDRVPGLDSRAAHLRQLMADRRVEARAYTREHGEDDPDISGWAWSPASVE
                     SAS"
     gene            19359..20444
                     /locus_tag="VFV59_01995"
     CDS             19359..20444
                     /locus_tag="VFV59_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324629.1"
                     /translation="MRVLVLNPGSSTLKASLLDPARDDPPDIGVAEWPAGRDADAPQV
                     VADALAQLPSTADAVGYRVVHGGLRYRSATPVDEELLDAVEALDALAPLHNHRAALVM
                     RAARDALPGPPHVACFDTAFHATLPEEAWRYPLPGDWVDRWEIRRFGFHGLSVAWSTR
                     RAAELLDRPGEALRTVVAHLGSGCSVTAVDGDRSVATSMGMTPYEGLMMGTRSGSVDP
                     GILLHLLRQGVALDELDEGITRRSGLLGLAGRPGAREVEEMARSGDAAAQLALDAFAR
                     SAAAGIAAAATALPAIDALVFTGGIGEHSSSIRAGIVRRLATLGVPGELRVPDGDGVL
                     ADGPPAVLVVEAREDRVIAAEVAAVVG"
     gene            20513..20914
                     /locus_tag="VFV59_02000"
     CDS             20513..20914
                     /locus_tag="VFV59_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324630.1"
                     /translation="MKLLLAAFLAAHALLHASYLSPAPPRTAGGPEWPFQLAGSWLVT
                     GLGLDPAPARALGTALVLTTVVLLVGAALATIGWLVPESWWVGLVTAGAAASLVTLAL
                     YFHPWLVLGVAIDVVLLWAALVSRWEPSSIG"
     gene            complement(21125..21541)
                     /locus_tag="VFV59_02005"
     CDS             complement(21125..21541)
                     /locus_tag="VFV59_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5324631.1"
                     /translation="MSVASPDLRVGNLMTIDPVVIDPEAFAAEAEALLKTYRISGLPV
                     VHDGTLVGVLSQTDLVVARSSAMIGVNWDRLKVRHLMTSPAVTVHVDARIADAAREMT
                     TRHIHRLVVVDDEDQPIGVVTPLDLLRSLVSEADAG"
     gene            complement(21636..22856)
                     /locus_tag="VFV59_02010"
     CDS             complement(21636..22856)
                     /locus_tag="VFV59_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5324632.1"
                     /translation="MTGSVLYRGGALADGRSSELRRDVSILVRDGRIAWIRPRDGEEA
                     VGDAEVIDAAGSAFVPGLVDCHSHVTGPGGANWIARFSDPAPALLAVAEDNARIASAA
                     GVRWMRDVGSPIGMDPVTGRERALALAVRDSWRDRADRPYLRAAGSWVFKRGSSPIPN
                     AIEAETGDELLAGAIGQLDAGADLVKLYMDGPDPETSPWTAGEVARVVDAVHARGARV
                     AAHAGRLDGARAAVRGGVDSVEHGFVLDDDVAAEMAQRGTVLTSTLAVMHSWLTFGAT
                     TTIPRFATQEGRSAIEARLETARASVAAARRAGVSIATGSDFGGGSLRANQLAWEVAS
                     LVRAGLEPHEALASATWRGGDLIGEPEAGRIHEGGPADFFCVHGDPLSDPDALWRVWR
                     TAWPQPGVVTSVPR"
     gene            complement(22853..23818)
                     /locus_tag="VFV59_02015"
     CDS             complement(22853..23818)
                     /locus_tag="VFV59_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324633.1"
                     /translation="MTGSQRAAVIGGTWLIGLGLVFLVQQALDVPWGQAWPLFLVLAG
                     VGTGVSALVGMAGRARPLAVAWALVWPLILVIAGVLLFLDFAELAPIDAWGLLVQWWP
                     VILIVLGGLVLLGAVWPTGRGVEEGVSVPLEAGEGEVRLRFGAGELEVGRGTPGRLVE
                     GTFEGGVIRRDLGPGAVELSADAAGMWPWVDRRLRWRIGLAPDVPLSLRVEGGAYRGF
                     LDLSELAVRALTVKTGASDTRIVLPRDVARCEVRIEAGAAQVSVDVPPEVAARIHSTM
                     ALGTTRVDESRFPRAADGWASPDLAGAARVAEIWVNGGVGTVRIS"
     gene            23884..24534
                     /locus_tag="VFV59_02020"
     CDS             23884..24534
                     /locus_tag="VFV59_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU5324634.1"
                     /translation="MTARRGRGRPPHPDVLTPAEWQVLDWLRHGMRRAEIARRRGTSV
                     DAVKYHLANISDKLGVRGGELRRWPGIPATSQLSRSRTTNPMTDTDSPIRLGPIGQVS
                     LSIRDVARAERFYGETLGLLHVFTFGDLAFFDAGGVRLYLHRKDEADWRPSSVLYFLV
                     DDIHRTQEALAADGVHFTGAPHVIYTDDATGTEEWMTFFEDGEGNMLALMSRVSPG"
     gene            24565..25794
                     /locus_tag="VFV59_02025"
     CDS             24565..25794
                     /locus_tag="VFV59_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324635.1"
                     /translation="MEPSARGGWRSLLLVYGITSTVEAAGVSQIFAYLPLRLGEVGLP
                     AADVPVFTGVFTSLIFVFGILLVPFWGVWADKYSRRAVIVRSAVVEAVVFAGVAWARE
                     PWQLAVALLLTGLQLGNTGVMLAALRDVTPSGRIGTMSAIFGATSPIGFAVGPVLAGL
                     LVDGAGLSLSAVFWLSAALSAGIAVLLLAATPEVRPEVVPTGRVITLATRAVTGVLRD
                     AGVRRLFVVFGVAILANQMFRPFVPILVERIHGTAAGLASAIALVTGTAALAGALVSP
                     VSGPLGDRIGFRPVLAVSLVAGGVAVGLMPLATGVLILAVLAAIYAAFQAAVQAMMFS
                     LIATEAPPERRSATLNLVLLPLYVAGILGPSAGAVVVAVTGVEGVFPAAALVFLLGGL
                     GVTGVLARVRRPRRGGA"
     gene            complement(25798..27000)
                     /gene="glgA"
                     /locus_tag="VFV59_02030"
     CDS             complement(25798..27000)
                     /gene="glgA"
                     /locus_tag="VFV59_02030"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEU5324636.1"
                     /translation="MRALILTNEFPPDIYGGAGVHVAELTRHLRSLIELDVRTFGSLA
                     EESAGWRVQGYPPAHDLADADERLRPMLAALSRDVAMVADPVNADVVHVHTWYTHFAG
                     ILARLAYGLPLVLTVHSLEPLRPWKREQLGGGYDVSAWVERTAIESADAIVAVSRGTR
                     ADVLRLFDVVPERVHVIHNGIDADFYRPDHGTDALERHGVDPMVPYVLFVGRITRQKG
                     IVHLVRAIQHLDPGIGVVLCAGQPDTPEIAREVTEGVRAAQATRDTVVWIGEMVSREE
                     VRQLYSHAAVFCCPSVYEPFGIINLEAAACETPVVASAVGGIPEVVVDGETGLLVPVE
                     LSPDDPMRPVDADRFERNLAGAINALMADPAARDAMGRAARRRAVSTFGWDAIARQTV
                     DLYRTLIA"
     gene            complement(27010..27480)
                     /locus_tag="VFV59_02035"
     CDS             complement(27010..27480)
                     /locus_tag="VFV59_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5324637.1"
                     /translation="MVTTSQPEPSGAAHPGPGPGRPLAFVVDDDPSALELLRAVAQDA
                     GWAVRGFTRLRDVQDALRRQSPALIVLDDDLPDGRGGDLARELRRDARLRGVPLLVCT
                     GAHAMRRAEIGAWAPVVSKPFDIVEMERLLADAADAADARRHVGHAATDIERAG"
     gene            27654..28592
                     /locus_tag="VFV59_02040"
     CDS             27654..28592
                     /locus_tag="VFV59_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324638.1"
                     /translation="MALISTRALTKRYPGGVTALDGLSVEIDEGVIGLVGANGAGKST
                     LIKVLLGLLSPTSGNAEVLGLDVATHGGQIRERVGYMPEHDCLPPDVSATEFVTYMAR
                     VSGLPAGAARERTADVLRHVGLYEERYRQMGGYSTGMKQRVKLAQALVHDPRLLFLDE
                     PTNGLDPAGRDEMLALIERIGREFGMTVVVASHLLGEIERVASWLVAIDAGRLLRTAP
                     LASFTERTGQLVVETEEETDALVAALTARGIEARAHGRLATVELGGGGADAVYDAVRD
                     EAVARDLPLVRIEHRRHQLEDLFRDPATPTAGGGSA"
     gene            28589..29473
                     /locus_tag="VFV59_02045"
     CDS             28589..29473
                     /locus_tag="VFV59_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5324639.1"
                     /translation="MSASPETAGTIYDIGYRHYDGPRLGRRGAVSAIVRAGLRALFGL
                     GRSGRAKILPWGTLALALLPAVIAVAIRVLAGDIIELYSYDNYLWEIGALLPIFLAAQ
                     APELVVNDVRHSVLPLYFSRPISRLDYVLAKGGALALALLSITLLPVLLLFLGRVLAA
                     EDIVAAFGDEAGSLVKIVGNALLHAVVLASIGLAICAVAGRRAYAAGAVLALFLIGGV
                     VSASFQEQGGALADWAPFVNPLSILDGTREWLFGGVSPNSPVRAADVPGPMYAVATLI
                     LLGVSWAVLAVRYRRIAT"
     gene            29470..30381
                     /locus_tag="VFV59_02050"
     CDS             29470..30381
                     /locus_tag="VFV59_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324640.1"
                     /translation="MSVLKLEAVSRWYGNVVAVNDVTFDVGPGITGLLGPNGAGKTTL
                     LHMMAGFLAPSAGAVQLDGEPTWRHPSLYRRIGFVPEREAVYAFLTGRQFVLASARLQ
                     GLDDPEGAADRAIETVDLVDAAGRNIGGYSKGMRQRIKVAAALVHDPGVLILDEPFNG
                     MDPRQRLHMMDLLREMAAAGRTILFSSHILEEVERLSGRVLVIVAGRLAASGDFRRIR
                     RLMTDRPHSFTIRSSDDRSLARELLAAPEVEGVELTDGALTVRTVDYGALTRTVGPAA
                     QRARASLYELRPADESLESVFSYLVRR"
     gene            30378..31106
                     /locus_tag="VFV59_02055"
     CDS             30378..31106
                     /locus_tag="VFV59_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324641.1"
                     /translation="MTATLAALTIRALLNRRRTLLLVLLAALIVVVAALFRLSDPSPQ
                     EASRFTGRLLADFGLAVLLPLVSVIVGTATIGSELDDGTIIYLLSKPIARVRIGLVKL
                     VVAWLVTILIVVPAILLAGLVGNGGDSALGLAYAVAAVVGALEYTAIFAALSLVTSRA
                     LVVGLAYVVIWEGVIAGLFAGTRTVSVRQHALAIADAFGAQGSVSAALELGTAVTVTV
                     VVTLLAAALMIRRLQGVELRGETP"
     gene            complement(31117..33204)
                     /locus_tag="VFV59_02060"
     CDS             complement(31117..33204)
                     /locus_tag="VFV59_02060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEU5324642.1"
                     /translation="MTDAARTQTVHRGVSAATESLRDAVARVAAVARDRAVFESAALL
                     IPIGLVGLTHATGPMLWLIASAYLVASAWLAVRAHERGGSAGWTEARILLAVGMVAAG
                     QLLTGTTGLLSVVYLPIIALAAFGSPRLLVLALVASVLAHLTSETIVRHAFLESVERA
                     LGFASAAGLVAFGTRGEVQRLERARHRLRRAVRSDRRRARHIAGIEAIGRVLAGQGPT
                     PAAFDDVVERISTEFGYRYVSVYLGDDRLVTLVAQRGYTELVETFDGERGIVGRVMRT
                     QRPVLVADVSRDPDYWVLNPDVTSEICTPMLAGGEFLGFLNVESTDRRLDTTDLRLMT
                     TIADRLAAALIIGRERQRISERADLFHHLHEFSEAVSSTLDAEQLHRAIARRASLVVP
                     ADVVTLHLLDPEHGRYLLRAIEGNSYLALGSEVRPGEGIAGRAVRDRTMVIDDAAPQL
                     PTLRTVAAGFLAEEPPPPMLGASVPLVRDGAVLGALTLMRHDRARRFTELERDALAML
                     AQQAVLAVTNVSLHAEVAELAMRDPLTGLFNRRYLDPAVQQLFARQARLPVEERVPIA
                     AIMFDLDHFSQLNNRHGHQIGDAVLRTFGEILRSRMRTTDLVGRFGGEEFVAILYRAS
                     LDDAMRIADEVREQLTATPIPGSDGEELRASVSAGCATVGPDQESPEELLRAADVALY
                     MAKRAGRDRVCAA"
     gene            33299..34333
                     /locus_tag="VFV59_02065"
     CDS             33299..34333
                     /locus_tag="VFV59_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU5324643.1"
                     /translation="MDLPPPWGHHWAGRIDEHVIDSQALTGNPLGDPHVRPLLVYTPP
                     GYERGERRYPSVYVIQGFTGQVDMWRNRKAFQPSALEELDAWFADESHAPAIVVFVDA
                     WTSLGGSQFVDSPGTGRYATYVSDDVVEFVDARYRTLADAAHRGVAGKSSGGYGAMIL
                     PMLRPDRFGGLATHAGDALFEHCYLPDFREAVRALRDAYDGSYDAFWSDFRSRPGRSR
                     RTDGPLINTWAMAACYSAEPDGTVRLPFETDTGRLIPEVWERWLAWDPVRMAESRNEA
                     LRDLRAAWIDAGRSDDFYLDLGAQAFRDALVRAGVAEDRIHFELFDGSHSGIDWRYPM
                     AIGWLAERLS"
     gene            34441..35304
                     /locus_tag="VFV59_02070"
     CDS             34441..35304
                     /locus_tag="VFV59_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU5324644.1"
                     /translation="MTFRPDAQLDPGRVTDARGRRVRGGGIAVGGGLGGLVLVALYLL
                     MGGNLGDLAGGAGAPAEGPVGSQLAAECDTGEEANQRQDCRIVGTINSLDAYWTEAFQ
                     ASNQQLQLPEAELFTDATQTACGGATSAVGPFYCPLDQTIYLDLGFYDDLQNRFGAEG
                     GPLAEEYVIAHEYGHHIQNLLGALDSNRDTGAEGGAVRTELQADCYAGVWAGNAVDTG
                     YLQPLTDEQIAQALNAAEVIGDDRIQEQTQGQVNPETWTHGSSEQRQHWFSVGLQEKN
                     PNACDTFNSDI"
     gene            35374..>36981
                     /locus_tag="VFV59_02075"
     CDS             35374..>36981
                     /locus_tag="VFV59_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5324645.1"
                     /translation="MDDSPFGSSAFPPIGDYAFLSDCETTALIAPSGNVEWLCLPRMD
                     SPSVFGAMLDRDAGGFRFGPSDVRVPADRRYLPGTMVVETSWGSPTGWIIVRDVLLMG
                     PWRHDETRSKTQRRPPTDYDASHILLRIVRCVNGEMQLTLDCEPVFDYGRQFGTWAYT
                     EDGYHQVACRSDASDVELVLTSDLNLGIEGPAVQARTLLKEGDVRFCALSWNGADVPR
                     TSQDAYQRLVWTAHHWQHWLARGTFPDHPWRSFLERSALTLKSLTYAPSGAVVAAPTT
                     SLPETPGGERNWDYRYSWIRDSTFALWALYTLGFEWEANDYLSFIADVAERDEQLQIM
                     YGIDGERALTEQVLEHLSGYEDARPVRIGNAAYGQKQHDMWGAVLDSVYLHTTSRDHL
                     DERIWPILVRQVEAALEHWREPDRGMWEVRGEPKHFTSSKLMCWVAADRGAKLARLRD
                     DDEHAKRWQQAADEIHADICGHGLDKRGVFTQHYETEALDASLLLMPLLHFLPPDDER
                     VRRTVLTIADELTDDGLVLRYRTDQTDD"
BASE COUNT         4986 a        13675 c        13169 g         5151 t
ORIGIN      
        1 atgtacgcgg tcggtgacgc gaccgacctg ccgctgtcga aggccgggtc caccgcccac
       61 ttcgaggcgc ccatcgtcgt cgagcgcatc gtcgccgggg tggagggtcg cgagcccgat
      121 gccgtcgccg gcaactacac cggcaaggtg atgtgcttct tcgaggtcgg tgacggcaag
      181 ggcaccctgc tccagttcga ctacgagcac cccccgaggc cgccgaagcc gaaccggctg
      241 tggcacctgg gcaagatcgt cttcaacaag acgtactggc acacggtgcc caagggacgg
      301 gtctagcggc ctccctcgag gcgcctggca acgggcggca acgcgcctgc gcgatcgcga
      361 gcgacacgaa gcggagaggc tgacgacggc ctcctcgtcg gtctggagga ccgattcgcc
      421 cctgggtccg ccggctcggg tcgggtgccc gacggcggcc cgacgggcgc gtgtgcaatc
      481 cgtcgttgcc tctccgttgc cgccatcctg ggcccgcggg ccgcggatcc acagggccat
      541 tcggacccac gcggcgcgac cgatgaccca ccgccaccgc ccggtcgctc cctagactgc
      601 tgccatgcgc atggtgagcg cggccgaggc cgtgaccggg atccagtccg gcgaccagat
      661 cttcgtccag ggcggggccg ccaccccgac gccgctgatc caggccctgg tggctcgcgc
      721 cgaggagctg cgcgacgtcg gcatcatcca cgtgcacacc gagggtccgg caccccacct
      781 ggcgcccgag atggcgccgc actttcggca ccgggcgctg ttcatcggcg ccaatgcgcg
      841 tgaggccgtc aacgacgggc gggccgacta cgtcccggtc ttcctctcgg acatccccga
      901 gctgttcacc aatggcgtgc tgccgctcga ctgggtcttc ctgaacgtca ccccgcccga
      961 tgcccacggc ttctgctcgc tcggcacgag cgtcgactgc acgctggcgg ccgcgcgcgc
     1021 cggtgcacgg gtcgtcgcgc aggtcaaccc ggccatcccg cgcacgttgg gcgattcgtt
     1081 catccatgtc gacgagatcg acctgggcgt atcggtcgat cgtccgccgt acgagcacca
     1141 cctcgatccg atcggcgacg tggaacggcg catcggcgat tacgtggccg agctggtgcc
     1201 cgacggggcg accctgcaga tgggcatcgg tgcgatcccg accgcggtgg gactggcgct
     1261 gcgccacaag aaggacctgg gcgtccacac cgagctcttc accgatccgg tcctcgacct
     1321 ggtcgaggcc ggtgcgatca ccggcgcggc gaaggagatc aaccgataca agatcgtgac
     1381 ggcgttcctg atgggcagcc ggcggctgta cgagtggatc gacgacaacc cgatggtcga
     1441 gatgcggccg gtcgactaca cgaacgacac ggcggtcatc cgccgcttcc ggcgcatggt
     1501 ggcggtgaac tcggccatct cgatcgacct caccgggcag gtgagcgcgg actccatcgg
     1561 cacccggttc tactcggggt tcggcgggca gatggacttc atgcgcgggg cggcgctggc
     1621 ggaggaggga cgcgccatca tcgcgctgcc ggccaccgcg gccggcggga ccgtgtcgcg
     1681 gatcgtgccg accctgacgg agggggccgg cgtggtcacg tcgcgcgccc acgttcggac
     1741 cgtggtcacc gagttcggcg tcgccgagct gttcgggaag agcgtccgtg aacgcgccgc
     1801 cgcgctcatc gccgtcgccc accccgactt ccgcgacgag ctgtgccacg aggcgaagcg
     1861 ccgctaccac gtgtagcgcg cgcgtggtgt cccgttcgcc cacgggccaa tcggcccgcg
     1921 agaagggtcc ccccatccct gccgaagctg ctcgcctcgc gcgcacgctt ccgccatgag
     1981 ctcgaagcac gctgcgccgc ccgccgcgcg gttccccggc accccggccg tcatggacgg
     2041 ctcggaggcg atcgccgatg tcgagacccg gatcgccgag ggagcctgcg cctacccgat
     2101 caccccatcc acgaacatgg cggccgcctt ccaggctgcc gtcgccgacg gccgcaccaa
     2161 cctgtggggg acgccgctgt cgttcctcga gctggagtcg gagcactcgt ccgccaccgc
     2221 ggccgagggc tacgcgctcg ccgggggccg cgtgaccaac ttcacggccg ggcagggcct
     2281 ggtgctgatg aaggaggtcc tccacgtcat cagcggcaag cggctgccgg ccgtgttcca
     2341 cgtcggggcg cgcgccctga ccagccaggc gctgaacatc cacgccggcc acgacgacct
     2401 gatggcggtg gccgataccg gctggggcat gctgatcgcc cgcaacgcgc aggaggccgc
     2461 ggacctgacg gccatcgggc ggcgcgtggc cgaggagacg gagacgccgt tcttcgtggc
     2521 gcaggacggg ttcctcacga cacacaccct ggagaacgtg cagcgacccg aggacgacct
     2581 gctgcggacg ttcgtgggtg atccgcggga gcgcatccgt gactacttcg ccccgtccga
     2641 ggcgctcatg accggtgtcg tgcagaacca ggacgcctac atgaagggca agatcgggca
     2701 gcgcgccttc tacgaccgcc tgcacggcgc cctcgtcagc gccatgaccg agtggaccga
     2761 gctcaccggt cgcccctacg caccgatcag cgcccatcgc accgaggatg ccgagcacat
     2821 cgtcgtgtgc atgggcacca tggccgacac cgccatcgcg gtggtggatc ggctgcgcgc
     2881 cgcgggccgt cgcgtcggct gcctgacggt gacgtcgttc cggcccttcc ccgcggaaga
     2941 gctgggggag gcggtgcgaa acgcctccgc cgtcgccgtc atcgagcgca ccgacgagcc
     3001 cgcggcgcac gacaacccgc tgacgcgcga gctgaaggcc gcgctggccg acgtggcgat
     3061 gagcggggcg cgcatcccgt acgtcgtctc cgcctcggcg gggctgggct cgcgcgacgt
     3121 cgcggccggg gacatcgccg ccgtcttcga ctggatcgcg gatccggagg ccgtgcggca
     3181 gcgccgctac gccgtcctgg gcatccgcca tcccctggcc ctcgagcgga tcccgatcga
     3241 catccgaccg gacggcgcgt acagcctgcg cggccactcc atcggcggct tcggatccgt
     3301 cacgaccaac aagctgctgg ccaccctgat gggcgacctg ttcggtctcc acgtgcaggc
     3361 ctacccgcgc tacggctcgg agaagaaggg tctgccgacc acctactacc tgaccatcgc
     3421 cgacgggccg atccgccagc acggcgagct cgacaccgtc gagttcgtcc cgctctacga
     3481 cgtcgccgcc ttccggcagg gcaatccgct gacgggtctc agcgaccgcg ggacggtctt
     3541 cgtccagacg cacctcggcg accctgaggc gatctgggga tcggtgcccg tcgccgcgcg
     3601 gacccagatg ctcgagcgcg gcctgcgcct ctgggcgctg gacaccgccg ccctggcaca
     3661 gcgacacgcc ccgcgtcccg acctgctggt gcgcatgcag ggcgtcgccc tggcgggcgt
     3721 cttcctgcgg gtgtcgccct tcgccgagcg tgcgggcttc gaccgcgaga cgctcctcgc
     3781 cgctctgcgc acgcagctcg agcgcttctt cggcaagcgc ggccgcgccg tggtcgacgc
     3841 gaacctggcc gtcatcgccg aggcctacga cagcgtgatc gacgtgaccg atgcggtcgt
     3901 cgccaccgac cagaccctac ccgcgccacg aatcctggag gttgtttcat gacccgctcg
     3961 ctcaccgtcg gggcgctgat gagcaccgac cccatcgtcg tcccggccga tgccccgctg
     4021 acccaggcgg ccgagctgct cgagcggcac cacatccatg gcctgcccgt cgtcgacgcg
     4081 ttcggtggcg tggtcggcgt cgtgagccag accgacatgc tccgcgcccg gacgacggac
     4141 gatctgtgga gccggtggcc cggcctcaag gtgcggcacc tcatgtcgag cccggcgctg
     4201 accatcagcg cggatgcgga cgtggccgaa gcggccaacc ggatggagct ggagcgcgtg
     4261 catcggctgg tggtggtcgg cccggacggt cgctcgccgg tcggcgtgct gtccaccacg
     4321 gacctggtgc atgggatgct cgaggaggcc cgggcatgac cctcactacc gatccgcttc
     4381 tgaaggagcc acgcgaggag aaggagctgc acgaggcccg actcgcgacg acggccccgc
     4441 tcgcgttcga cgtggaggcg tacgtcgacg acttcaacag cgtggtcgtc ggggcctacc
     4501 gcgcgggcag cgccgatgcc gggctgccgg ccgatgcatc cgtcgcgcgc agcgtgatcc
     4561 cgccggggac ggccgccgtc cgcgacttct cgaccctggc tccgcagctg cccgccttca
     4621 tcagcgagcg ctgcgtgggc tgcatggcgt gcgtgaacgc ctgtccggac agcgcgatcc
     4681 tggcggtcgc ccagcccggc agcgtgctgg agccggccat cgccgcctgc gtcgccgaga
     4741 cgggcgacgg ggcgggcgcc agcgtggagg cggacctgcg atcccacttc gtgaccacca
     4801 ccaagtacgc gaccgtcccg gccaagaagg gcctggagcc ggccgagttc gggatcttcg
     4861 tggaccccgt gcactgcaag ggctgcgcgg aatgcgtcga ggtctgctcg gcgctcggcc
     4921 acgacgcgct gctgatgacc gacaagctgc ccgtcgaaga ggtctcgggc accagtacgg
     4981 tcgaccgcta cgcccgcgac ttccgcttct tccgcacgct gccggtgacc cctgagccgt
     5041 atcgcaacga gaaggcgctg gccgacgtga tgctcggcga gcacgccttc ggctacgtcg
     5101 gcggcgccgg gtcgtgctcg gggtgcgcgg aggcgaccgc cctgcgcatg ctgctcgcgg
     5161 cgacgcgcca ggtccacggc ccggagtcga tgggcatcgt ggcggccacc ggctgcaaca
     5221 cggtgttcgg cagcacgtac ccgttcaacc cgttcctggt gccctggacc aactcgctgt
     5281 tcgagaacgg cccggccgat gcgctgggca tccgcgcccg gtgggaccag gcggggcatg
     5341 ccgaccgacg gctgtgggtg gtgggtggcg acggtgcgat gtacgacatc ggcttccagt
     5401 cgctctcacg gctcgtggcg tccggcgccg acgtcaaggt catggtcctc gacacgcagg
     5461 tgtactcgaa caccggcggc caggcgtcga ccgcctcgtt cggcgggcag gtgacgaagc
     5521 tgtcggccta cggcaagagc ctgcacggcc gcccggagca gcgcaaggag ctcgcccgca
     5581 tcctcatggc ccacggcgag gtctacgtgg cccagaccac gccggcgcac atcaaccact
     5641 tctaccggtc cgtcatggac gcgaacgcgt accccggccc ggccgtggtg gtggtgtaca
     5701 cgccctgcat gccggagcac ggcatcgccg acgacgcggc ctcgcgccag gcgaagctgg
     5761 ccgtcgactc gcgggccttc ccgctgttca cctacgaccc gcgtcgcggg ccgtcgatgg
     5821 cggagcggct cagcctccag ggcaacccgg cgctcaagga ggactgggag cggctgcacg
     5881 acggtggcgc gaccgacttc gtcgcgttcg cgcggaccga gggccgcttc gcgccgcact
     5941 tcgcgccgga cggaacgccg agcaacgagc tgctggccac gcaggccgac cggctggcca
     6001 actggcgcct gctccaggag ctggccggga tccgctgatc cggcgagctc agtggcggcg
     6061 ctcgcgacgc gggcgccgct gctcgctcct agcgcggagc gcgcgcaggc ggcggtcctg
     6121 cctggagcgc ggcctggatc ggcgtcgatg cccgtcccgc gccatcgcgt gcgcctcagg
     6181 caacggattg cgggtgccgc cggcacctgg tgtccatcag gcgcagccag tcgtgcacga
     6241 acagcggctt gaggagccga gcgtcacgcc ggcgctcggt gaggtcggcc gccgccggat
     6301 ccgcggtgag gaggatcacg gcggcgtcgg acccgaccag ccctcggagc tcgtcgtcgt
     6361 cggggcgggc acgccccgtc acgtcgacga acgccacgca ccaccggaag cggcggtacg
     6421 gcgagcgggt gaggcgcgcc agtccatcgg ccgccgcaac cgcgtggccg cggtcgagga
     6481 gggcgcgcag gaggagcgtc ctgacgcgga cgtcctcgtc gacgacgagc acccgatggc
     6541 gcgtcgtcga gatggggatc gtggcgcggt gcgcgtccgc catccgggcg gcctcgcgga
     6601 agccgtcgcc ggcaacctcg atcaggcgct cggtctgcgg atgcattccg cgtcctcccc
     6661 ggatcgcggc gggctcggac cgccgctgat gcggtcgatc caagcaccac ggcccggcgg
     6721 ggtctgtccg cgggcggacg gacggcgtac gattgcatcg gccccgcgag cggtgggagg
     6781 acgtggtgga gccggatggg aagcagccga gcgacccggt gaccggtgat ccggtcgtgc
     6841 gcgggagcgc catcaacggg atcctcgccc gcctgccgcc acgagagctg gccgccttca
     6901 ccgtcgctgc ccggcccttc cgggcgccca agggacagct cctcgcggca ccgggcacca
     6961 cctccgcggc catcttcccg attcgcggcc tcatctcgct cggcctcacc gacgagcgcg
     7021 gcgagacgat gacggtggac gtggtcggcc gcgagggcat ggccgggctg tggctctacc
     7081 tcgacgacgt cccgacgccg tacctcgtca gcgccctcgt cccgctcgcg gggtggttga
     7141 tcgacgccgg tgcgctgcgc cgcgtcgcgg cacccgggac gacgctggcg acgctcctcg
     7201 gccggtacgc gcagtaccgc ctggcactcc gggcgcacct catgcactgc aaccggaccc
     7261 acgagctcac gccccggctg gcgcggttcc tcgacggctg cgcccgcgcg agcgggtcgg
     7321 ccgagctcga gctgtcgcac gaggacctgg cctccggggt cggtgcgcac cgaccggcag
     7381 tgacggatgc gctccaccgg ctggccgccc ttggtgccgt gcgcagcggc cggcggctgg
     7441 tcgaggtgtc gtcgcccgcc gggctccagg cggcggcgtg cacctgcgcc gcgaccatcg
     7501 ccgagctcca ggagcggttc gaccgacccg actagcgcgt gccggatggc ccgcgaggcg
     7561 cttccatccg gccctcaaac cgccgccctc gcgccggtac ggtcgaagcg tgaatgagca
     7621 tgacttcgtc gctgagctgg gcgcgacctg gttcggagcg ggactctcgc tccacgcgcg
     7681 cgcccgcctc ggggagtgcg cgcggctcct cacccatccc gccaacgacg tcctgctgcg
     7741 cgagggcgac gacaccccct tcttcgggat cctgacgcga gggcgcgtcg gcctccgcat
     7801 gcgggttccg gagcgtgggc cggtgacgat cctgaccgtc gagcccggcg acgtggtggg
     7861 ctggtcggcg ctggtgcccc cgcaccgggc gacctccacc gccgtcacga tcgagccgag
     7921 cacgctcatc gagttcgagc cccggcggct ccgggagctg ctcgagcacg acctcgccct
     7981 gagcgccacg ctctacccgc ggctgctcga ggccatcgga cggcgcctct ccgccacgcg
     8041 acagcagctg ctggacctct acgcccagca ggcggagccg gtgccatggt gaccgatgcc
     8101 ggcttccttc cccgggccga tctcgaccgc ctgatcggcc tcctgcgcga gggtggacgc
     8161 accgtcatcg ggccgacgct cgacgacggg gccctggtct acgccgagat cggatccgca
     8221 gacgacctgc ccaccggctg gaccgatgac gccgctccgg gccgctatcg gctggtgcgt
     8281 gacgaggggc agcgcacgtt cgggctcggg ccgccgccgc agggcctgaa gcgcttcacc
     8341 ttcccgtccc gcgccccact ggccgtggcg gagcggaccg ccgagggcgc cgtgaccttc
     8401 accgcggccg tccccgaccc gccgcggctc gccttcctgg gcgtccgccc ctgcgaggtg
     8461 gcggcgttgc tcgtccagga ccgcgtcctg gccggcggcc cgttcgtgga cgaggattac
     8521 cgcgccaggc gacaggcgtc gctggtggtg gccgtcgcct gcacccgctc gggcagcacc
     8581 tgcttctgcg cctcgatggg gaccggaccc gaggtgaccg ccggtcacga cctggccatg
     8641 accgagctgg acgacggatt cgtcgtctcc gccggcagcc gtgccgggga acagctcctc
     8701 gcgcgcctcg cgctcccggc ggtcacgccc gaccagcacg cgaccgcgac cgcggccgtg
     8761 gccggtgcgc gcgcggcgca gggccgccag gtcgtgacga ccggcctgcg cgatcggctc
     8821 atggaccagc tggaccatcc ccgctgggcc gaggtggccg agcgctgcct ggcgtgcgcg
     8881 aactgcacca tggtctgccc cacctgcttc tgcaccagca ctgccctcga ctccgacctg
     8941 gacggggcgg cgacgcgcat ggagcgccgc tgggacagct gcttcacggg cgacttcgcc
     9001 cgcgtggcgg gcggcaactt ccgcccgcgg cgccaggacc gataccgcca gtggctcacg
     9061 cacaagttcg cgacctgggt cgaccagttc gacaccttcg gctgcgtcgg ctgcgggcgg
     9121 tgcatcacct ggtgcccggt cggcatcgac gtgcgcgagg agctcgccgc catcgcgccg
     9181 ccctacgagg agctgccgac cgcgacgccg gtggtggcgg gctcgaccag cgcctactcg
     9241 accgccgtcg tgcggtcggt gacgccggag acggccgaca cctccaccct cgccctggtg
     9301 cccggcgacg acgcgctcct ggccgcacgg ccggggcagt tcgtgatggt cgagcagcct
     9361 ggcttcccgc cgctgccgat cagcgtctcg cgcgtcgccg acggcgccct gtggctcacc
     9421 atccgcgccg cggggcccgc caccacggcc accatcgcgc tgcggcccgg tgcgcagctg
     9481 ggcctgcggg gaccgctggg ccggggctgg gacatccagc gcgcggtggg ccgtgacgtg
     9541 gtcatcgtgg ccggcggcat cggcctggcg ccggtgcggc cgatcatcga caccgtcctg
     9601 cgcgatcgcg agaagttccg gcacgtgatc ctcgcctacg gggcgcggac cccggcggac
     9661 cggctctacg tggacgagat ggccgactgg gccgatcggg gcatggtcgt gaccgagatc
     9721 gtcgaccgcg ccgggccgga gtggctcggc cgcgtcggca tcgtcacgca cctgctcgac
     9781 cagcgcgact tccacgaccg accggcgacc gccttcgtgg tgggcccgga gcggatgatg
     9841 gaagccaccg tcgccaccct ggcggagcgc ggcgtgccgc gcgagcggat cttcgtcagc
     9901 ctcgagcgcc acatgcagtg cggcatcggg ctgtgcgggc actgccagat gggcccgttc
     9961 ttcatctgcc gtgacggccc ctgcttcgcg gtgagcgagc tcggcgacct gttcggcagg
    10021 gagggcatct gatgatcagc gatcgacccc gcgtcggcgt cgtcaagctc gcctcgtgcg
    10081 acggctgcca gctgacgctg ctcgacctgg aggacgagct gctgccgatc gtcggccgct
    10141 tcgacctcat cgagttcccc gaggctacct cgctccgctc ggccgggccg tacgacgtgc
    10201 tgctggtcga gggagcggtc agcacgccgg agcaggcgga agagatcgtc catctccggg
    10261 cgcgctcgcg gctgctggtc agcatcggcg cctgcgccac cgccggtggc atccaggcgc
    10321 tgcgcaactg gaccgatgag ccggactggc gcgcatcggt ctaccccgaa gccgggtgga
    10381 tcgagtcgct ggcgacggcc accccggtga gcgagtacgt cccggtcgac gccgagctgc
    10441 gcggctgccc gatcgccccc ggccagctcc tggagctgct gacggcgctg cgcatcggcc
    10501 gccggccgca gctgcctgcc gagtcggtat gcctggcctg caagcgtcgc ggcaacgagt
    10561 gcgtgctcgt ggcgcgcggc atcccgtgcc tcgggccggt gaccgtggcc ggctgcggcg
    10621 ccctgtgccc gaccttcggg cgcggctgct acgcctgctt cgggccacgc gaggaggcga
    10681 acgtcgcctc gctccacggc tggctcgccg ccgaccgctc gacccaggag gtggatcggc
    10741 tcttcgccgg cttcaccgcc tggtccccgc cgttccgcga gcccgccgca cgggcggtcg
    10801 cggtggccga gacgcaccac ccacgaaagg acgatcccga tgcatgaggt cgaggccgcc
    10861 cccgaggccg tcaccgaggt cggcgacgtc acgcggttcg cggtgaagca gctgacccgc
    10921 gtggagggcg agggccggct ggacctggcc gttcgcgacg gcgtggtgac gcaggcccac
    10981 ctcgagatct tcgaggcgcc gcgctacttc gagcggatcg tcgtgggacg gacgccggac
    11041 gaggtgatcg acatcgtcgc gcgcatctgc ggcatctgcc cggtggcgta ccagatgtcc
    11101 gcggtccacg ccttcgagga cctgttcggc gtcgccgtgg atccgcagat ccgcgccctg
    11161 cgacgcctct tctactgcgg tgagtggatc gagagccacg ccctgcacgt ctatctgctc
    11221 cacgcccccg acttcctggg ctacccgagc gccatcgagc tggccgccga tcacggcccg
    11281 atcgtcgagc gtggcctgcg cctgaagaag gtcggcaacg acctggtcgc gctgctgggc
    11341 ggacggccga tccacccgat cagcgtccgc gtgggcggct tctcccgcgt gccgaaggcg
    11401 gcggagctgg agccgatgcg cgcccgcctg gcgggcgcgc tggtggaggc gatcgagacc
    11461 gtgcgctggg tcgccaccct cgagcctccg ccgtacgcgc gggtgccacg gctcgtctcg
    11521 ctggtgcacc cgaccgagta ccccatgaac gacggccgga tcacgtccag cgacggcatc
    11581 gccgtcgcgc cgagcgcctg gaccgatgcc ttcgaggagc aacaggtccc gtggtcgaac
    11641 gcgctccagg cccgcgcccg ggacggggtg ccgtacctgc tcgggcccac ggcccgcgtg
    11701 gtgctcggcg gctccacgct gcaccccgag gccgccgccg cgctggccga gaccgggctc
    11761 ggcgacgagc tccagaacaa cgtgttccgc agcatcgtcg cccggggcgt cgagctggtc
    11821 cacgccatcg ccgaggccat cgacatcatc gacgcctacc gccggccggt cgagccgaag
    11881 gtcgcctggg aggcgcgtcc cggcgtggcc gcatgggcca cggaggcgcc ccgcggcctc
    11941 ctcttccacc gctacgaggt ggacgaggcc ggtctcgtca gccgcgccca gatcgtgccg
    12001 ccgaccagcc agaaccagcg ggccatcgag cacgacctgg cgaccttcgc ccccagcgtg
    12061 ctcgagctcc cgcacgagga ggcgacccac cggctggagc agctgatccg cagctacgac
    12121 ccgtgcatca gctgcgcgac ccacttcctc gagctcaagg tcgacgagcg ctgatgacgg
    12181 tgaccgagat gcggcacgtg acgatcctcg tctgcggcga gccgatgcgc ggtgacgacg
    12241 cggtcggcga ggccgtggtg cgggcgcttc ccgccaccac gctgcagctc ggcgaggtct
    12301 gtcacgtcgg cgggctcatg cccgacgacc tgatggcggc cggcgcaccg gtgatcgtcg
    12361 tggacgcggt gcacggcctg ccggccggga ccgtggtcga cgtcccgctg gccgcgctgc
    12421 cggagctcgc cgccgtgggg gccacgcccg agtccacgca cgccatcccg ctccccacgg
    12481 tcctcgggat cgtggagcgg ctggccgggg agctgcccga gggccgcttc atcggcgtgg
    12541 ccgccggcac ggacctcatc ggcgcgccgc tctcggagcc agtccgtgag gccgtcgacg
    12601 tctgcgcggc ccggctcggg cactggatcc gaacgctggc ccatccgacg gtggggacat
    12661 cctgatgtgc ctcaccgccc cggtccgcgt gatcgacgtg agcggcacca ccgccaccgt
    12721 ggacgccgcc ggccggcgcc gcaccgcgtc tgccctcgcc gtccccggca tccggcccgg
    12781 ggactgggcg ctgttgtccg ccggaaccct cgtgcgcgtc atcgatccgg ccctcgccgc
    12841 ggagatcgcc gacgccttcc gtaccgcgac aggagaacaa ccgtgatcga aaggattcgc
    12901 gccgccgatc gaggcgggca tcggctcacc atcgggcttg ccctggcggg gatgaccgcg
    12961 ctggtgagcg gcgtctccgt cttcctcaac gccaccgccg tccgctcggt cggcgacccg
    13021 gtgctgttca ccaccctcaa gaacggcgtg gcggccctgg tcctcgtggc cgtcgcgctg
    13081 gccctcgtgc cccgtcccgc ggcgaccatc gcggcgctca gcggccgcac gaagctgggt
    13141 ctgctggccc tcggcgtcgt cggcggcagc atcccgttcg tgctcttctt caccggcctg
    13201 gccagcgcca gcgcgcccgc ggcggcggtg atccacaaga cgatgttctt ctgggtcgcg
    13261 ctgctcgccg tcgtcctgct tcgggagcgc ctgggcctgc tccaggtggg tgccctgggc
    13321 gtgctgctcg tcagccagct gctgatccaa ccgccggacg gcatcagctg gacctcgggc
    13381 gagacgatga tcgccatcgc caccgccctg tgggcagtcg aggtcatcgt cgccaagcgg
    13441 ctgctgcgcg cggtcccgtc ccccatcggc gcggcggcgc gcatgggcat cggcctggtg
    13501 ctgctggtgg cctacctggc ctggaccggt gggctcgcca agctgggcgg cctggggctg
    13561 gagcaatggg cctgggtcct gggcaccggc ctcctcctgt cggcctacgt cgccacctgg
    13621 tacgccgccc tgcgacgcgc cccggcgacc gcggtgacgg ccgtcctgac ggtcgccgcc
    13681 ccgatcacgg ctgcgctgca gctcgtggcc aacggccagg tgccggcggc cggcgccacc
    13741 atcggctacg ggctgacgct cgtcgccggc gccgtggtgg ccgtcatggt cctgcgccga
    13801 cccgccgcca gccgcgaagc ggcgctcgcc tgaggagacg aggcgtggcg gccacgacga
    13861 tccccgcggc ggcgccggac gccgtcgcgg gtccgatcct cttcgcccgt tacgcctacc
    13921 cgcccaaccg cctcggcctg tgcggcccgg acgacgcccc gggcctgctc gacggcgcca
    13981 cggccggggc cgaccggcag ctccgctcgc tggctctcgg cttcgagggc gcgtacccgt
    14041 acctgcgcct catcgccgaa gccaacggca tcgccgaccc gctcgacgcg cgggtggtgg
    14101 aggcgtactg gctcggcagc ccgctcacct cggccgttcg cccacgcccc ctgcaccgcg
    14161 atctcgagga ccggttccgc ggccgcatga cggcgagcga ctggcgctgg ctggaggcgg
    14221 ccgtcggggc ggcctcgcga ccggtccacg cgttccacgt gctcgaggtg ttcccgcgcg
    14281 tcggcctgct tcgcggcggc ccggccgacc cgatcgtgga gacgatggac tcgtgccgca
    14341 tccgcccggc ccgcgtgctg gccgtggacg gcggccacct caccgtggcg gcgcgtccgc
    14401 tgacgctggt cgacggccag ctgcagctcg gcgacgagcg ggtggagtcg gccgatgcct
    14461 ggcgtggctc cgacggcgtg ctggacggcg tgcaggtcgg cgactgggtg tcgctgcact
    14521 ggggctgggc ctgcgatagg ctcagccgac cgcagctcgc ccgcctcgtt cgctggacgg
    14581 gcgccgccat ccggaccgcg aaccgcagca tgtgaggggt atgaccgcca gttccgatat
    14641 ccagcgctgg cacgacaacc tgcagggcga ggtcgacggc tcgcacgtgt accgcgccat
    14701 ggccgcgaat gccggagacg accggctcag tgagctctat gagcgcctgg cggcggtgga
    14761 ggatcgccac gcgcagctct ggcaagagcg cctgaccgat gcaggcgcgc gcgccgcgct
    14821 gcgcccgacg tggcgggccc gggtgctggc cgcggtcgca cgtcgcttcg gcgcgggcgt
    14881 cgtggcgccg gtcatcgcct cccaggagcg gcagggccgc acgatgtacg acacccagcc
    14941 cgaggcggcc ggcacgtcgc tccccgccga cgagcgctcg cacgcccggc tgctgcggac
    15001 cgtcacggga ggcgcggccg gggcgaccct ggcccggttc gaagggcggc accgcgccat
    15061 cggcggcaac gccctccgcg ccgcggtgct cggcgcaaac gacggcctgg tctccaactt
    15121 cagcctggtc atgggcgtgg cgggcgccag cagcggcggt gagccggtgc tgatcgccgg
    15181 catcgcaggg ctgctggccg gctccctctc catggccctc ggcgagtggc tcagcgtcca
    15241 gagcgcgcgc gagctgtacg ccaaccagat cgcgaccgag gcggaggagc tgcgcacctt
    15301 ccccgccgaa gaggaggaag agctgcgcct gatctacgag gccaagggcc tcgacccgga
    15361 ggaagcccgc agcctcgcac aacggatcat cgccggcgac ccgcaggtcg ccctcgacac
    15421 catggcccgc gaggagctgg gcatcgaccc cgaggagctg ggtggctccg cttggacggc
    15481 ggcgttcacc tccttcgggc tgttcgccgc cggagccatc atccccgtgc tgcccttctt
    15541 cgtcaccgcc ggcatgaccg cgatcatcgc ctcggccgtg ctgtcgggtg tggcgctgtt
    15601 cggcatcggc gccctcatca ccatcctgac cggccagccg gcgctggcct ccggcgcgcg
    15661 ccagctgggc ttcggcctcg cggcggccgc catcaccttc gccatcggca gcctgctggg
    15721 cacggccctc ggctgacggt cccgcactag gaggaacctt cccatggacg agcgacgcca
    15781 ggagttctcg gtcaacggcg accagctggt ggccaaggtc cgcgaactga tcgaggaggg
    15841 caacgtccgc cgcctcatca tcaagaacga ggacggtcgg accctgctcg aggtcccgct
    15901 caccctcggc gtgatcggcg cggccctgct gccggtcttc gcggccatcg gcgcgctggc
    15961 ggcgctggcc acgcgctgca cgatcgtcgt cgagcgggcg gaggcggcgg aggtcccgcc
    16021 ggccacgacg cccgagagca cgaccgacct gacctgaacc gacgggcggc cgccgaactg
    16081 accgacgcgc gcggccccgg ttgccagtga ccggggccgc gcccgcaccg gtggtcgtgg
    16141 gccgccgcca gcacgccaag gggagctcga catgcacgtc ctcgtcaccg cggcctccaa
    16201 gcacgagtcc accgccgaga tcgccgatgc catcgccgcc tcgctccgcg ccgccggcat
    16261 caccgtcgac cgctcaccgc cggaccagat cgcctcggtc gacggctacg acgcggtcgt
    16321 cgtcgggagc ggcgtctacg ccggacgctg gctggacccg gcgcgggcgt tcgtggagcg
    16381 ccacaccgac gcgctgcgac ggcgcccggt gtgggtcttc tccagcggcc cgctcggcga
    16441 cccgccgctc ccggcggatc cgtcggccga tggcgtgagc ctggccgacc gcatcggggc
    16501 gcgcgagcat cggacgttca gcggcaggct ggatcccggc aggctcgggt tcctggagcg
    16561 caccgtcgtc aaggccttcc gtgcgcccgc cggcgactcg cgcgactggg atgcgatccg
    16621 gacctggggc acggagatcg ccgggcttct cgccgcgaca gccaagggcg gctgagaggc
    16681 cgcgggacct cgcggctgac ccgtggggcg ggttcaacgg accgctgaag cgggcccagc
    16741 gtgggctctg gcggtgaatg acccgtgggg cgggtcaacg gaccgctcaa ccgggcctgg
    16801 cgggtgctcc ggcggtgagt gacccgtggg gcgggtcaac ggaccgcggc ccgaaggggg
    16861 agctgcgacc gaccaatggg accgttcggg ctggagaccg gcaggctgct acgctggccg
    16921 tcccaccgcg tcgaggagct tcccgaccat gaacgctcgc atcgccgagc ccgcgaccgt
    16981 cgccccgccg aagacggatg agctgcgccg catcgacgcc tactggcgcg cggcgaacta
    17041 tctctccgtc ggccagatct acctgctgga caacccgctc ctgcgcgagc cgctgcgtcc
    17101 cgagcacatc aagccgcgcc tgctgggcca cttcggcacg gtcccgggac tgaacctggt
    17161 ctacgtgcac ctgaaccgtg cgattcggca acgggacctg aacgccatgt acgtcaccgg
    17221 gcccggccac ggcggtccgg gcctggtcgc gaacgcctac ctcgagggga cctacagcga
    17281 ggtctacccc accatcagcc aggacgtcga cggcatgcgg atgctgttcc ggcagttcag
    17341 cttccccggc ggcattccca gccacgtggc gccggagaca cccggctcca tccacgaggg
    17401 cggcgagctg ggctacgccc tggtccacgc ctacggcgcc gccttcgaca acccggacct
    17461 ggtggtcgcc gcggtcgtcg gggacggcga ggccgagacg ggcccgctcg ccgccagctg
    17521 gcactcgaac aagttcgtgg accccgcgcg cgacggtgcg gtcctgccga tcctccatct
    17581 caacggctac aagatcgcga accccaccgt cctggcccgc atcccggagg aggagctggt
    17641 caagctgctc gagggctatg ggcactcggt cgagatcgtc tccggcgacg acccgatgaa
    17701 ggtgcaccgc gccttcgcgg cggccctgga tcgcgcgctc gacgcgatcg ccgacatcca
    17761 gcgccgggct cgcagcggca agggcgaggc gaagcggccg cgctggccga tgatcgtgct
    17821 gcgcacgccg aagggttgga ccggtccgga ggaggtcgac ggcaagccgg tcgagaacac
    17881 gtggcgctcc caccaggtgc cgatggccga cgtccgcgac aaccggaagc acctcaagct
    17941 gctcgaggac tggatgcgct cgtatcggcc cgacgagctg ttcgacgagt ccgggcgact
    18001 gcggccggag ctcgcagggc tcgccccgcg tggggagcgg cggatgagcg ccaacccggt
    18061 cacgaacggt ggcgcggtgc tccagggtct ccggctgccc gacttccggg actacgcggt
    18121 cgaggtgacc gagcccggcg gcgtgacgag cgaggcaacc cgcgtcctgg gtgagttcct
    18181 gcgcgacgtg atcgaccgca acccgaccag cttccgcctc ttcggtccgg acgagaccgc
    18241 ctcgaaccgg ctgcaggcgg tcttcgagaa gaccgacaag gcgtggatgg ccgagcggct
    18301 gccggtcgac gagcacctcg cccctgacgg ccgggtgatg gaggtcctca gcgagcacct
    18361 gtgccagggc tggctggagg gctacctgct caccggccgc cacgggctgt tcgactgcta
    18421 cgaggccttc atccacatca tcgactcgat gttcaaccag cacgcgaagt ggctgaaggt
    18481 gacccgcgac atcccgtggc ggcggccgat cgcgtcgctg aactacctgc tctccagcca
    18541 cgtgtggcgg caggaccaca acgggttcag ccaccaggac cccggcttca tcgaccacgt
    18601 ggtcaacaag aagagcgaga tcatccgggt ctacctgccg ccggacgcca actcgctgct
    18661 cagcgtcgcc gaccactgcc tgcgcagccg gcactacgtg aacgtcatcg tcgccggcaa
    18721 gcagccgatg ccgaactggc tgtcgatgga cgacgcgatc ctgcacgcca cgcggggcat
    18781 cggcatctgg acctgggcca gcaacgacga ggggcaggag cccgacgtgg tgatggcgtg
    18841 cgcgggcgac gtgccgacgc tcgagacgct ggcggccgtc gacatcctcc gccgccatct
    18901 ccccgacctg aaagtgcgcg tggtgaacgt cgtcgacctc atgcgcctcc aggatccggg
    18961 cgagcatccc cacggcctct cgaaccggga gttcgacgcg ctgttcacca ccgatcggcc
    19021 gatcgtcttc gcctaccacg gctatccgtg gctcatccat cggctcacct accggcggac
    19081 gaaccacgac aacctgcacg tgcgcgggta caaggaggag ggcacgacga ccacgccgtt
    19141 cgacatggtc atgctcaacg acctcgaccg ctaccacctg gtcatggacg tcatcgaccg
    19201 ggtgccaggc ctggacagcc gtgccgcgca cctgcgtcag ctgatggccg atcggcgggt
    19261 cgaggctcgc gcgtacacgc gcgagcacgg cgaggacgac cccgacatca gcggctgggc
    19321 ctggtcgccc gccagcgtcg agagcgcgag ctagcccgat gcgggtgctc gtgctgaacc
    19381 ccgggtcgag caccctcaag gcgagcctcc tggacccggc gcgcgacgat ccgccggaca
    19441 tcggcgtggc ggagtggccg gccggccgcg acgccgatgc tccccaggtc gtggccgatg
    19501 cgctcgccca gctgccatcg accgccgatg cggtcggcta ccgggtcgtc cacggcgggc
    19561 tgcgctaccg cagcgccacg ccggtcgacg aggagctgct cgatgccgtc gaggcgctcg
    19621 atgcccttgc cccgctgcac aaccaccgtg ccgcgctggt catgcgcgcc gcccgcgacg
    19681 cgctgcccgg ccctccgcac gtcgcctgct tcgacaccgc gttccacgcg acgctcccgg
    19741 aggaggcgtg gcgctacccg ctgccggggg actgggtcga ccgatgggag atccgccgct
    19801 tcggcttcca cggcctctcg gtcgcatggt cgacccggcg tgccgccgag ctgctcgatc
    19861 ggcccggcga ggccctgcgc acggtcgtcg cgcacctggg atccggctgc tccgtcaccg
    19921 cggtcgacgg cgatcgatcg gtggccacga gcatggggat gaccccctac gagggcctga
    19981 tgatgggcac ccgctccggt tcggtggacc ccgggatcct gctgcacctg ctgcggcagg
    20041 gggtcgccct ggacgagctc gacgagggca tcacgcgccg gtccggcctg ctggggctgg
    20101 ccggcaggcc cggtgcccgc gaggtggagg agatggcccg ctccggcgac gccgccgcgc
    20161 agctcgccct ggacgcgttc gcacggagcg ccgcggccgg catcgcggct gccgcgaccg
    20221 ccctccctgc tatcgacgcg ctcgtgttca ccggcggcat cggcgagcac tcgtcgtcca
    20281 ttcgagccgg catcgtccgc cggctggcaa cgctcggcgt gcccggtgag ctccgcgtgc
    20341 cggatggaga cggcgtcctc gcggacgggc cgccggcggt gctcgtcgtc gaggcccggg
    20401 aggaccgcgt catcgctgcc gaggtcgccg cggtcgtcgg gtgacgaagc gcacgcggcc
    20461 ggcgtgacct ccggcactgg ggatctcggg tggggccgcg cagcctgacc gcatgaagct
    20521 cctcctcgcc gccttcctgg cggcgcacgc cctgctccac gcgtcgtacc tgagccccgc
    20581 gccgccgcgc accgctggcg gcccggagtg gccgttccag ctcgcgggct cgtggctggt
    20641 caccggcctc ggcctcgacc ccgccccggc gcgcgcgctc ggaaccgcgc tcgtcctcac
    20701 aaccgtcgtg ctgctcgtcg gcgcggccct cgcgaccatc ggctggctcg tccccgagag
    20761 ctggtgggtg ggcctggtca ccgcgggggc cgccgcctcc ctcgtgaccc tcgccctcta
    20821 cttccacccg tggttggtgc tgggtgtcgc gatcgacgtc gtgctgctat gggccgccct
    20881 cgtgagccgc tgggagccct cgagcatcgg ctgatggacg cgggcgctcg cccgcgaccc
    20941 ccggcgccca cggaggtcga gtggctcgtc tgtcgggcgc atctccgcga ggtgcgggac
    21001 ggccgtgtgc ggtgtccgct cacccaccgc gcggtgcggg cactcgagtc gtgcctcgag
    21061 tgcccggagc tggagctgat cgccaacgag cgcgccctgg ccgagtgcgg gacgccggcg
    21121 tcgctcagcc ggcgtcggcc tcgctgacca gcgagcgcag caggtcgagg ggcgtgacga
    21181 cgccgatcgg ctgatcctcg tcatcgacca cgaccaggcg gtggatgtgc cgggtcgtca
    21241 tctcgcgcgc ggcatcggcg atgcgggcgt ccacgtggac ggtcacggcc ggggacgtca
    21301 tcaggtggcg gaccttgagc cgatcccagt tcacgccgat catggcgctc gagcgagcga
    21361 cgacgaggtc cgtctggctc agcacgccga ccagcgtgcc gtcgtggacg accggcagcc
    21421 cgctgatccg atacgtcttg aggagcgcct ccgcctcggc ggcgaacgcc tccgggtcga
    21481 tgaccaccgg gtcgatcgtc atcaggttgc cgacccgcag gtcggggctc gcgacgctca
    21541 tggcgtacct cctcgggtgt cgggtgcggg ccctaccgag ccgtaccgcc agtctgccgt
    21601 ccccgccggc gccggttgac ggccgaacgg cccgatcagc gcgggaccga ggtcaccacc
    21661 cccggctggg gccaggcggt gcgccagacc cgccacagcg catcgggatc ggagagcggg
    21721 tcgccgtgga cacagaagaa gtcggcgggc ccaccctcgt ggatcctccc cgcctccggc
    21781 tcaccgatca ggtcgccgcc gcgccacgtg gcggacgcca gcgcctcgtg cggctcgagc
    21841 ccggcgcgga ccaacgaagc gacttcccag gcgagctggt tggcgcgcag cgagccgcca
    21901 ccgaagtcgc tccccgtcgc gatggacacg ccggcacgac gggcggccgc gacggacgcg
    21961 cgggccgtct ccagccgcgc ctcgatggcc gaacggcctt cctgggtggc gaaccgcggg
    22021 atggtcgtcg tcgcgccgaa ggtcagccac gagtgcatga cggccagcgt ggacgtcagc
    22081 acggtcccgc gctgcgccat ctcggccgcc acgtcgtcgt ccagcacgaa cccgtgctcg
    22141 accgagtcga caccgccgcg caccgcggcc cgggcaccgt caaggcgtcc ggcatgcgcc
    22201 gccacgcgcg ccccgcgggc gtggaccgcg tccacgacgc gcgccacctc gccggcggtc
    22261 cacggcgacg tctcggggtc cgggccgtcc atgtagagct tgacgaggtc ggcgccggcg
    22321 tccagctggc cgatggcacc ggccaggagc tcgtcgcccg tctcggcctc gatcgcgttc
    22381 gggatcgggc tgcttccgcg cttgaagacc caggagccgg cggcgcgcag atagggacgg
    22441 tcggcgcgat cgcgccagga atcccgcacc gccagggcca gcgcgcgctc gcggccggtg
    22501 accgggtcca tgccgatcgg cgagcccacg tcgcgcatcc agcgcacgcc ggcggcggac
    22561 gcgatccgcg cgttgtcctc cgccacggcc agcagcgccg gggcgggatc cgagaaccgc
    22621 gcgatccagt tcgccccgcc gggcccggtg acgtggctgt ggcagtcgac caggcccggg
    22681 acgaaggccg agccggcggc gtcgatcacc tcggcgtccc cgacggcctc ctcgccgtcc
    22741 cgcggccgga tccatgcgat gcgcccgtcg cgcaccagga tggagacgtc acgccgcagc
    22801 tcggatgagc ggccgtcggc cagcgccccg ccgcggtaca ggaccgaacc ggtcacgaga
    22861 tgcggaccgt gcccaccccg ccgttgaccc agatctccgc cacgcgcgcc gctcccgcca
    22921 ggtccggcga ggcccagcca tcggcggccc gcgggaagcg cgactcgtcg acgcgggtcg
    22981 taccgagggc catggtcgaa tggatgcgcg cggcgacctc gggaggcacg tcgacgctca
    23041 cctgggcggc gcccgcctcg atgcggacct cgcagcgggc gacgtcgcgc gggaggacga
    23101 tccgcgtgtc gctggccccg gtcttgacgg tgagcgcacg aacggcgagc tcggagaggt
    23161 ccaggaagcc gcggtacgcc ccaccctcga cccggagcga gagcggcacg tcgggcgcca
    23221 gaccgatgcg ccagcgcagc cgacgatcga cccacggcca catgccggcc gcatcggccg
    23281 agagctcgac cgctcccggt ccgaggtcgc gccggatgac gccgccctcg aacgtcccct
    23341 ccaccaggcg gccgggcgtg ccccggccga cctcgagctc gccggcgccg aagcgcagcc
    23401 gcacctcgcc ctcgcccgcc tccagcggga cggaaacccc ctcctcgacg ccacgtccgg
    23461 tcggccacac ggcgccgagc aggaccagcc cgccgagcac gatcaggatc accggccacc
    23521 actgcaccag cagcccccac gcgtcgatgg gcgccagctc ggcgaagtcc aggaacagca
    23581 ggacgccggc gatcaccagg atcagcggcc acaccagcgc ccaggcgacg gccagcggcc
    23641 gcgcgcggcc cgccatcccg accagggccg acacgccggt ccccacgccg gccaggacca
    23701 ggaagagcgg ccacgcctgg ccccacggca cgtcgagcgc ctgctggacc aggaacacga
    23761 ggcccaggcc gatgagccac gtcccgccga tcaccgcggc ccgctgcgaa ccggtcatgc
    23821 ggcgatgcta cgccacgtcc acgggtaggg taattcgcga gcggcgcgtc tacgctgtcc
    23881 gccatgaccg cgcgacgagg ccgaggtcgc cctccgcatc ccgatgtcct gacgccggcc
    23941 gaatggcagg tgctcgactg gctgcgccac ggcatgcgtc gcgcggagat cgccaggcgg
    24001 cgaggcacca gcgtcgacgc ggtcaagtac cacctcgcca acatcagcga caagctcggc
    24061 gtccgcggcg gcgagctgcg ccgctggccc ggcatcccgg ccaccagtca gctctcccgc
    24121 tcgaggacca cgaatcccat gaccgatacc gactccccta tccgactcgg ccccatcggc
    24181 caggtctcgc tctccatccg cgacgtggcg cgcgccgagc gcttctacgg cgagacgctg
    24241 gggttgctgc acgtcttcac cttcggcgac ctcgcattct tcgacgcggg cggggtccgg
    24301 ctctacctgc accgcaagga tgaggcggac tggcggccgt catcggtcct ctacttcctg
    24361 gtcgacgaca tccaccgcac gcaggaggcg ctcgccgccg acggcgtgca cttcaccggg
    24421 gccccgcacg tgatctacac cgacgacgcc accggcaccg aggagtggat gacgttcttc
    24481 gaggacggcg agggcaacat gctggcgctg atgtcgcgcg tctcgccggg ctgagggccg
    24541 ccggcacacc gatagactcc gcgggtggag ccgtccgccc gcggaggctg gcgctcgctg
    24601 ctgctcgtct acggcatcac ctcgaccgtc gaagcggccg gcgtcagcca gatcttcgcc
    24661 tacctgcccc tgcgcctcgg cgaggtgggg ctgccggccg ccgacgttcc ggtcttcacc
    24721 ggcgtcttca cctcgctcat cttcgtcttc ggcatcctgc tggtcccgtt ctggggcgtc
    24781 tgggccgaca agtacagccg ccgcgccgtc atcgtccgca gcgccgtggt ggaggccgtc
    24841 gtcttcgccg gcgtggcgtg ggcccgggag ccgtggcagc tcgccgtagc cctcctgctg
    24901 accggcctcc agctcggcaa caccggcgtc atgctggccg cgctgcgtga cgtcaccccg
    24961 tcggggcgga tcggcaccat gagcgcgatc ttcggcgcga ccagccccat cggtttcgcc
    25021 gtcggcccgg tgctggccgg cctgctggtg gacggggccg ggctgtcgct gtccgccgtc
    25081 ttctggctct cggcggcgct gtcggccggc atcgccgtgc tgctgctggc cgcgacgccc
    25141 gaggtgcggc cggaggtcgt ccccaccggc cgggtcatca ccctggccac gcgcgccgtg
    25201 accggcgtgc tgcgggacgc cggcgtgcgt cgcctgttcg tcgtcttcgg cgtggcgatc
    25261 ctggccaacc agatgttccg tcccttcgtg ccgatcctgg tcgagcgcat ccacggcacg
    25321 gcggcaggcc tggccagcgc catcgcgctc gtgacgggca cggcggccct ggcgggggcg
    25381 ctggtgtcgc ccgtctcggg tcccctgggg gaccgcatcg gcttccggcc ggtcctggcc
    25441 gtctcgctgg tcgccggggg cgtggcggtc gggctcatgc cgctggccac cggcgtcctc
    25501 attctggccg tgctcgcggc gatctacgcg gcattccagg cggcggtgca ggccatgatg
    25561 ttcagcctga tcgccaccga ggcgccaccc gagcgccgct ccgcgacgct caacctggtg
    25621 ctgctgccgc tctacgtcgc cggcatcctg ggaccgagcg ccggcgcggt ggtggttgcc
    25681 gtcacgggcg tcgagggcgt gttcccggcg gcggcgctgg tgttcctcct cggcgggctg
    25741 ggcgtgaccg gcgtgctcgc ccgggttcgg cggccgcgcc gcggcggcgc gtgatcctca
    25801 ggcgatcagg gttcggtaca ggtcgaccgt ctgccgcgcg atcgcgtccc acccgaacgt
    25861 gctgaccgcc cggcggcggg cggcccgccc catggcgtcg cgcgccgccg ggtccgccat
    25921 cagcgcgttg atggccccgg ccaggttccg ttcgaaacgg tcggcgtcga cgggtcgcat
    25981 cgggtcgtcc ggcgacagct ccaccgggac cagcagcccc gtctcgccgt cgacgacgac
    26041 ctccgggatg ccaccgacgg ccgatgcgac caccggcgtc tcgcacgccg ccgcctcgag
    26101 gttgatgatt ccgaacggct cgtagacgga tgggcagcag aacaccgcgg cgtggctgta
    26161 cagctgccgc acctcctcgc gcgagaccat ctcgccgatc cacaccacgg tgtcgcgcgt
    26221 cgcctgcgcc gcgcgcacgc cctcggtcac ttcgcgcgcg atctccggcg tgtccggttg
    26281 gccggcgcac agcaccaccc cgatgccggg gtcgaggtgc tggatggcgc gcaccaggtg
    26341 cacgatcccc ttctggcggg tgatgcgtcc gacgaagagc acgtagggca ccatcgggtc
    26401 gaccccgtga cgctcgagcg catcggtgcc gtggtccggg cggtagaagt cggcgtcgat
    26461 gccgttgtgg atgacgtgga cgcgttcggg cacgacgtcg aagagccgca ggacatcggc
    26521 gcgcgtgccc cgtgagacgg cgacgatcgc atcggccgat tcgatggcgg tgcgctccac
    26581 ccaggcgctg acgtcgtacc cgccacccag ctgctcacgt ttccagggcc gcaggggctc
    26641 cagcgagtgc acggtgagca cgagcgggag gccgtacgcg aggcgcgcca ggatgccggc
    26701 gaagtgggtg taccaggtat gaacgtggac gacgtccgcg ttcacggggt cggccaccat
    26761 ggcgacgtcg cgcgacaggg ccgcgagcat cggtcgcagg cgctcatcgg catccgccag
    26821 atcgtgggcc ggcgggtaac cctgcacccg ccagccggcc gactcctcgg ccagggagcc
    26881 gaaggtgcgg acgtcaagct cgatcagcga tcgcaggtgc ctggtcagct cggcgacgtg
    26941 aaccccggca ccgccgtaga tgtccggcgg gaactcgttg gtgaggatga gcgcgcgcac
    27001 gcgcccagcc tacccggcgc gctcgatgtc ggtggcggcg tggccgacgt gacgccgggc
    27061 gtcggccgcg tcggcggcgt ccgccagcag ccgttccatc tccacgatgt cgaacggctt
    27121 cgagacgacc ggcgcccacg cgccgatctc cgcacggcgc atggcgtgcg cgccggtgca
    27181 gacgaggagc gggacgccgc gaagacgagc gtcccggcgc agctcgcgcg caaggtcgcc
    27241 gccgcgcccg tccggcaggt cgtcgtcaag gacgatcagg gccggcgatt ggcgccggag
    27301 cgcgtcctgc acgtcgcgca gcctggtgaa gccgcgcacc gcccaccccg cgtcctgggc
    27361 gaccgcacgc aggagctcca gggccgacgg gtcgtcgtcc acgacgaagg cgagggggcg
    27421 tcccggcccg gggccggggt gggccgcgcc gctcggctcg ggttgcgaag ttgtgaccat
    27481 gatgcggttc tgactagcaa ccgacgtgcc gggccacccc gaaacgagcg gaaacctgcg
    27541 catcgatggc tgatccctgc ggattcggca agcggcaagc gaggttggcg ttcgatccgt
    27601 gacgtggagc gggtcgcgac ggcgcggcgc gtcgggccgt agactggcgg aacatggctc
    27661 tcatcagcac ccgagcgctg acgaagcggt accccggtgg ggtgactgcg ctcgacggcc
    27721 tgagcgtcga gatcgacgag ggcgtcatcg ggctggtcgg cgccaacggc gccggcaaga
    27781 gcaccctgat caaggtgctg ctcggcctgc tgtcccccac gtccggcaac gccgaggtcc
    27841 tcggcctgga cgtcgcgacc cacggcgggc agatccggga gcgcgtcggc tacatgccgg
    27901 agcacgactg cctcccgccg gacgtgtccg ccaccgagtt cgtgacgtac atggcgcgcg
    27961 tgtcggggct ccctgccggc gccgcgcggg agcggacggc ggacgtcctg cgccacgtgg
    28021 gcctgtacga ggagcgctac cgccagatgg gcggctactc gaccggcatg aagcagcggg
    28081 tcaagctcgc ccaggcgctg gtccacgacc ccaggctgct gttcctggac gagccgacca
    28141 acggccttga cccggccggc cgcgacgaga tgctggcgct gatcgagcgc atcggccgcg
    28201 agttcggcat gaccgtcgtc gtcgccagcc acctgctcgg cgagatcgag cgggtcgcgt
    28261 cgtggctcgt cgccatcgat gccggccgcc tgctgcgcac cgcgccgctc gcctcgttca
    28321 ccgaacgtac ggggcagctc gtggtcgaga cggaggagga gaccgatgcg ctggtcgccg
    28381 ccctgacggc ccgcggcatc gaggcgcggg cccacggtcg gctggcgacg gtcgagctgg
    28441 gcggcggcgg cgccgacgcc gtctacgacg cggtccgcga tgaagcggtc gcccgcgacc
    28501 ttccgctggt gcgcatcgag caccggcgcc accagctcga ggatctgttc cgcgatccgg
    28561 cgaccccgac ggccggtgga gggagcgcat gagcgccagt cccgagaccg ccggcacgat
    28621 ctacgacatc ggctaccgtc actacgacgg cccgcggctg ggacgccgcg gcgcggtgtc
    28681 cgccatcgtc agggccgggc tgcgcgcgct cttcggcctg ggtcgctcgg gccgcgccaa
    28741 gatcctgccg tggggcacgc tcgccctcgc gctgctcccc gccgtcatcg cggtcgccat
    28801 ccgggtcctc gccggcgaca tcatcgagct gtactcgtac gacaactacc tgtgggagat
    28861 cggtgcgctc ctgccgatct tcctggccgc gcaggcgccg gagctggtcg tcaacgacgt
    28921 tcgccacagc gtcctgccgc tctacttcag ccggcccatc tcgcgcctcg actacgtcct
    28981 ggccaagggc ggcgcgctgg cgctggccct gctctccatc acgctgctgc ccgtcctgct
    29041 gctcttcctg ggtcgcgtgc tggcggcgga ggacatcgtg gccgccttcg gcgacgaggc
    29101 cggctccctc gtcaagatcg tcggcaacgc gctcctgcac gccgtggtgc tggccagcat
    29161 cggcctggcg atctgcgccg tcgccggccg gcgggcgtac gccgcgggcg ccgtgctggc
    29221 gctgttcctc atcggcggcg tggtcagcgc cagcttccag gagcagggtg gggcgctggc
    29281 cgactgggcc ccgttcgtga atccgctgtc gatcctggac ggcacgcgcg agtggctgtt
    29341 cggtggcgtg tcccccaact cgcccgtccg ggcggcggac gtccccgggc cgatgtacgc
    29401 ggtcgcgaca ctgatcctgc tgggggtgag ctgggccgtg ctggccgtcc gctaccggag
    29461 gatcgccacg tgagcgtcct caagctcgag gcggtctcgc gctggtacgg caacgtcgtg
    29521 gccgtcaacg acgtgacctt cgacgtcggg ccgggcatca ccgggctgct gggcccgaac
    29581 ggcgcgggca agacgaccct gctgcacatg atggccggct tcctggcccc ctccgccggc
    29641 gcggtgcaac ttgacggtga gccaacctgg cggcacccgt ccctctaccg gcgcatcggc
    29701 ttcgtgccgg agcgcgaggc ggtctacgcg ttcctcaccg ggcgccagtt cgtgctggcc
    29761 tcggcgcggc tgcagggcct ggacgatccg gagggcgcgg cggatcgggc gatcgagacc
    29821 gtggacctgg tcgacgcggc gggccgcaac atcggcggct actcgaaggg gatgcgccag
    29881 cgcatcaagg tggccgccgc cctggtccac gatccgggcg tcctgatcct tgacgagccg
    29941 ttcaacggga tggacccgcg ccagcggctg cacatgatgg atctgctgcg cgagatggcg
    30001 gcggcgggac ggacgatcct cttctccagc cacatcctcg aggaggtgga gcggctgtcg
    30061 ggccgcgtcc tggtgatcgt cgccggacgg ctcgccgcct cgggcgactt ccggcgcatc
    30121 cgccgcctca tgaccgatcg gccgcacagc ttcaccatcc gctcgtccga cgatcggtcg
    30181 ctggcccgcg agctcctggc cgccccggag gtcgaggggg tggagctgac cgacggcgcc
    30241 ctcaccgtcc gcacggtcga ctacggcgcg ctgacccgca cggtgggacc cgccgcgcag
    30301 cgcgcgcggg cctcgctgta cgagctgcgg ccggccgacg agtcgctcga gtcggtgttc
    30361 agctacctgg tgcgccgatg accgcgacgc tcgccgccct gaccattcgc gccctgctca
    30421 accggcgccg cacgctgctg ctggtcctgc tggcggccct gatcgtggtg gtggcggcgc
    30481 tcttccggct ctcggaccca tcaccgcagg aggcctcgcg cttcacgggg cgcctgctgg
    30541 ccgacttcgg cctggcggtg ctgctgccgc tggtcagcgt catcgtcggc accgcgacca
    30601 tcggctccga gctcgacgac ggcacgatca tctacctcct ctcgaagccg atcgcccgcg
    30661 ttcgcatcgg cctcgtcaag ctggtggtcg catggttggt gacgatcctg atcgtcgtcc
    30721 cggccatcct gctggcaggg ctggtcggca acggtgggga ctccgccttg ggcctcgcgt
    30781 acgcggtggc cgccgtcgtc ggagcactcg agtacacggc catcttcgcc gccctctcac
    30841 tggtgacgtc ccgcgcgctg gtggtcgggc tggcgtacgt cgtgatctgg gagggggtga
    30901 tcgccgggct gttcgcgggc acccggacgg tctcggtgcg ccagcacgcg ctggccatcg
    30961 ccgacgcatt cggcgcgcag ggatcggtca gcgcggcgct ggagctgggc acggcggtga
    31021 ccgtcaccgt ggtcgtgacg ctgctggcgg cggcgctgat gatccgccgc ctccagggcg
    31081 tcgagctccg gggtgagacg ccgtagcgct gtcgcgctag gccgcgcaga cgcggtcgcg
    31141 ccccgcccgc ttcgccatgt acagcgcgac gtccgcggct cgcaacagct cctccgggga
    31201 ttcctggtcc ggaccgacgg tcgcgcagcc agccgagacg ctcgcccgta gctcttcccc
    31261 gtccgagccg gggatcggcg tggcggtgag ctgctcgcgc acctcgtccg cgatgcgcat
    31321 cgcgtcgtcg agtgacgcgc ggtagaggat cgccacgaac tcctcgccgc cgaaccgccc
    31381 gaccaggtcc gtggtgcgca tgcgggaacg caggatctcg ccgaacgtgc gcagcacggc
    31441 gtcgccgatc tggtggccgt gacggttgtt gagctggctg aagtggtcga ggtcgaacat
    31501 gatcgccgcg atcggcacgc gctcctcgac gggcagacgc gcctggcgcg cgaagagctg
    31561 ctgcaccgcc gggtcgaggt agcgacggtt gaagagcccc gtcagcggat cgcgcatggc
    31621 cagctcggcg acctcggcgt ggagcgacac gttcgtcacg gccaggacgg cctgctgcgc
    31681 gagcatggcg agcgcgtcgc gctccagctc ggtgaaccga cgcgcccgat cgtgccgcat
    31741 gagggtgagc gcgccgagca ccgccccatc gcgaaccagc ggaacggagg cgccgagcat
    31801 gggcggcggc ggctcctccg ccaggaaccc ggcggccacg gtgcgcagcg tcggaagctg
    31861 gggcgcggcg tcgtcgatga ccatggtccg gtcgcgcacg gcgcgtcccg cgatcccctc
    31921 gccgggacgg acctcgctgc cgagcgccag gtagctgttg ccctcgatgg cgcggagcag
    31981 gtagcggccg tgctcggggt cgagcaggtg cagcgtcacg acgtcggccg ggacgacgag
    32041 tgaggcccgg cgtgcgatgg cgcggtgcag ctgctccgcg tcgagcgtcg agctgacggc
    32101 ctcgctgaac tcgtggaggt ggtggaacag gtccgctcgc tcgctgatgc gctgccgctc
    32161 ccggccgatg atcagcgcgg cggcgaggcg gtcggcgatg gtcgtcatca gccgcaggtc
    32221 ggtggtgtcg agccggcggt ccgtcgactc gacgttcagg aagcccagga actccccgcc
    32281 ggcgagcatc ggcgtgcaga tctcgctcgt gacgtccggg ttcagcaccc agtaatcggg
    32341 gtcgcgagac acgtcggcga ccaggaccgg acgctgcgtg cgcatgacgc gacccacgat
    32401 cccgcgctca ccgtcgaagg tctcgaccag ctccgtgtag ccgcgctggg ccaccagggt
    32461 cacgaggcga tcgtcgccga ggtagacgga cacgtagcgg tagccgaact cggtcgagat
    32521 ccgctccacc acgtcgtcga acgcggccgg ggtcggaccc tgcccggcga ggacgcggcc
    32581 gatcgcctcg atgccggcga tgtggcgggc acggcggcgg tcggaccgta cggcgcggcg
    32641 caggcggtgc cgcgcgcgct cgagccgttg cacctcgccg cgcgtcccga aggcgacgag
    32701 gccggccgcg ctggcgaacc cgagggcacg ctcgaccgac tcgaggaagg cgtggcgcac
    32761 gatggtctcg gaggtcaggt gagcgagcac ggaggcgacg agcgccagca ccagcaggcg
    32821 cgggctgccg aaggcggcca gcgcgatgat cggcaggtac acgaccgaga gcaggcccgt
    32881 cgtgccggtc agcagctgcc cggccgccac catcccgacc gccaggagga tccgggcctc
    32941 ggtccagccg gccgatccgc cgcgctcgtg ggctcggacc gcgagccagg cgctggccac
    33001 caggtaggcg ctcgcgatca gccacagcat cggtccggtg gcgtgcgtca ggccgaccag
    33061 gccgatcggg atcaggaggg cggccgactc gaagaccgct cggtcgcggg caacggcagc
    33121 cacgcgcgcg acggcgtcgc gcagggactc cgtcgccgcg gatacgccgc gatggaccgt
    33181 ctgggtccgg gcagcatcgg tcatggacgc gacgagtctc gcgggagccg tcagggtcct
    33241 ccatggccca tcggtaccat gccgaccgtc cggcgtacgc ggctgctacc ctccaggcat
    33301 ggacctgcca ccgccctggg gccaccattg ggccggccgc atcgacgagc acgtgatcga
    33361 cagccaggcg ctcaccggca acccgctcgg cgatccgcac gtgcggccgc tgctggtgta
    33421 cacgccgccc ggctacgagc gcggcgagcg ccggtacccg tcggtctacg tcatccaggg
    33481 cttcacgggc caggtggaca tgtggcgcaa ccgcaaggcc ttccagccat cggcgctgga
    33541 ggagctggac gcgtggttcg ccgacgagtc ccacgcgccg gccatcgtcg tcttcgtcga
    33601 cgcctggacc agcctgggcg gctcgcagtt cgtggactcg cccggcaccg ggcgctacgc
    33661 gacgtacgtc agcgacgacg tggtggagtt cgtggacgcc cgctatcgca ccctggccga
    33721 tgccgcccac cgcggcgtgg ccggcaagag cagcggcggc tacggcgcca tgatcctgcc
    33781 gatgctccgg cccgaccgct tcggcggcct ggccacccac gcgggcgacg ccctcttcga
    33841 gcactgctac ctgcccgact tccgggaggc cgtccgcgcg ctgcgtgacg cgtacgacgg
    33901 ctcgtacgac gccttctggt cggacttccg gtcccgcccg ggacgaagcc gccgcaccga
    33961 cggtccgctc atcaacacct gggcgatggc cgcctgctac tccgccgaac cggacggcac
    34021 cgtccgcctt cccttcgaga ccgatacggg ccgcctgatc cccgaggtct gggagcgctg
    34081 gctggcgtgg gatccggtgc gcatggccga gtcccgcaat gaggcgctcc gcgacctgcg
    34141 agcggcatgg atcgatgccg gccggagcga tgacttctat ctcgacctcg gcgcccaggc
    34201 gttccgcgac gcgctcgtgc gcgcgggcgt ggccgaggac cgcatccact tcgagctgtt
    34261 cgacggatcg cactcgggca tcgactggcg ctacccgatg gccatcggct ggctcgccga
    34321 gcgcctctcg taggcgcccg cccgccgcga cgggggccgt gacgcgcggc tggcacgcag
    34381 gacgcatatg ctggctcggt cgcacgagcg accaagcgat tcccggagga gacacgatcc
    34441 gtgacctttc gacctgacgc ccagctcgat cccgggcgcg tcaccgacgc tcgtgggcgc
    34501 cgcgtgcgcg ggggcgggat cgcggtcggt ggcggcctcg gcggcctggt gctggtcgcg
    34561 ctctacctgc tgatgggtgg caacctcggc gatctggccg gtggtgccgg cgccccggcc
    34621 gaggggccgg tcggcagcca gctggcggcc gagtgcgaca ccggcgagga ggccaaccag
    34681 cggcaggact gccgcatcgt cggcaccatc aacagcctcg acgcctactg gaccgaagcg
    34741 ttccaggcct ccaaccaaca gctgcagctg cccgaggccg agctcttcac cgacgccacg
    34801 cagaccgcct gcggcggcgc cacgagcgcc gtcgggccgt tctactgccc gctggaccag
    34861 accatctatc tcgacctcgg cttctacgac gacctgcaga accggttcgg cgccgagggc
    34921 gggccgctgg ccgaggagta cgtgatcgcc cacgagtacg ggcaccacat ccagaacctg
    34981 ctcggcgcgc tggactcgaa tcgggatacc ggcgctgagg gcggcgcggt gcgcacggag
    35041 ctgcaggccg actgctacgc cggcgtctgg gccggcaacg cggtcgacac gggctacctg
    35101 cagccgctca ccgacgagca gatcgcgcag gcgctcaacg cggccgaggt catcggcgac
    35161 gatcgcatcc aggagcagac gcagggacag gtcaaccctg agacctggac gcatggctca
    35221 tcggagcagc gccagcactg gttctcggtg gggctgcagg agaagaaccc gaacgcctgc
    35281 gacaccttca acagcgacat ctgatccgat cggagcgact ccaggaggta gatcacgggc
    35341 aggaacggcg tccccaccgg ccgcaaccgc ttcatcgacg actcgccctt cgggtcgtcg
    35401 gcctttccgc ccatcggtga ctacgccttc ctgtcggact gcgagacgac cgcgctgatc
    35461 gcgccgtcgg gcaacgtcga gtggctgtgc ctgccgcgca tggactcgcc gagcgtcttc
    35521 ggagccatgc tcgaccgcga cgccggcgga ttccggttcg gcccgtccga cgtccgggtt
    35581 cctgccgacc gccgctacct gcccgggacc atggtcgtcg agacgagctg gggatcgccg
    35641 acgggctgga tcatcgtgcg ggacgtgctg ctgatggggc cctggcgcca cgacgagacg
    35701 cggtccaaga cccagcgacg tccgccgacg gattacgacg ccagccacat cctgctgcgc
    35761 atcgtccgct gcgtgaacgg cgagatgcag ctcacgctcg actgcgagcc ggtcttcgac
    35821 tacggccgcc agttcgggac ctgggcgtac accgaggatg ggtaccacca ggtcgcctgc
    35881 cgttcggacg cgagtgacgt cgaactggtg ctgaccagcg acctcaacct ggggatcgag
    35941 gggccggcgg tccaggcgcg cacactgctc aaggagggcg acgtccgctt ctgcgcgctg
    36001 tcctggaatg gggccgacgt cccgcgcacc agccaggacg cctaccagcg gctggtgtgg
    36061 accgcgcacc actggcagca ctggctggcc cggggcacct tcccggacca cccgtggcgc
    36121 tccttcctgg agcgctcggc cctgacgctc aagtcgctga cctacgcgcc gtccggtgcc
    36181 gtggtggcgg cgccgacgac ctccctgccc gagacgcccg gcggtgaacg caactgggac
    36241 taccgctaca gctggatccg cgactccacc ttcgccctct gggcgctcta cacgctcggg
    36301 ttcgagtggg aggccaacga ctacctctcg ttcatcgcgg acgtcgcgga gcgcgacgag
    36361 cagttgcaga tcatgtacgg catcgacggc gagcgagcgc tgacggagca ggtcctggag
    36421 cacctgagcg gctacgagga tgcgcgcccg gtccgcatcg gcaatgcggc ctacggccag
    36481 aagcagcacg acatgtgggg cgccgtgctg gactcggtgt acctgcacac cacctcgcga
    36541 gaccacctgg acgagcgcat ctggccgatc ctggtgcgcc aggtagaggc ggcgctggag
    36601 cactggcgcg agcccgaccg cgggatgtgg gaggtccggg gcgagccgaa gcacttcacc
    36661 agctcgaagc tgatgtgctg ggtcgccgcg gaccgcgggg cgaagctggc gcgcctgcgg
    36721 gacgacgacg agcacgcgaa gaggtggcag caggccgcgg acgagatcca cgccgacatc
    36781 tgcgggcacg gcctcgacaa gcgtggcgtc ttcacccagc actacgagac cgaggcgctc
    36841 gacgcctcgc tcctgctgat gccgctcctc cacttcctgc cgcccgacga cgagcgcgtc
    36901 cggcgcacgg tcctgaccat cgccgacgag ctgacggatg acggcctggt gctgcgttac
    36961 cgaacggacc agaccgatga c
//
LOCUS       DASTYP010000040        20457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_221230, whole genome shotgun sequence.
ACCESSION   DASTYP010000040 DASTYP010000000
VERSION     DASTYP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 20457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20457
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221230"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFV59_02080"
     CDS             complement(<1..917)
                     /locus_tag="VFV59_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5324646.1"
                     /translation="MPKPSYPPAPTSDQVDDYHGVAVADPYRPLEDTDAPETRRWIAA
                     ENDLTERVLSGLAARGEIRARLAELWDFPRAGAPIHRGDRWFQLRNTGLQDQDVLWVS
                     DAPDDPGRVLLDPNGLSQQGTRALSAMAISEDGERLAYAISDAGSDWRTWSVREVATG
                     DDLADRVTWSKFASAAWTADGEGFVYGRYPEPPPDAAYDAPNLQMELCYHRLGTDAAD
                     DVVVLSVPEHPDWGFEPMVTDEGRLLVVSVWRGTDPENRLWVADLAGGVDSIEVRRLL
                     DVPDARYEPIGAVDGTLYLLTDRDAPNGRV"
     gene            complement(950..1222)
                     /locus_tag="VFV59_02085"
     CDS             complement(950..1222)
                     /locus_tag="VFV59_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEU5324647.1"
                     /translation="MNEACTHLDQIHDVEPSADGCEDCLRIGSGWVHLRLCMTCGHVG
                     CCDQSPNRHATAHVHASGHPIIRSHEPGEEWGYCYPDDLYMETLPR"
     gene            complement(1290..1883)
                     /locus_tag="VFV59_02090"
     CDS             complement(1290..1883)
                     /locus_tag="VFV59_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEU5324648.1"
                     /translation="MTWTIDSAHSSVTFSAKHMMVTTVRGSMKIRDFDLDFDPDNLVD
                     SRVRVVLDAASIDTDQQMRDDHLRSADFLDVANHPDITFQSTEIVPRGGDEYEIRGDL
                     TIRGTTRPAVLKAEYAGTVTNMQGGKSAGFSATTKINREDFGLTWNVALEQGGLLVSK
                     DIKIEIDLEAMSSAAEVTAEAEAESEMDEREAARASA"
     gene            2019..2483
                     /locus_tag="VFV59_02095"
     CDS             2019..2483
                     /locus_tag="VFV59_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5324649.1"
                     /translation="MTIDAPQVSRHDPRISAWRTFLETHARVVRELEQELQAEADLPL
                     THYDVLVQLSGADARRLRMSELADRLLLSRSGVTRLVDRLVADGLVERVSCDSDRRGA
                     WASLTDAGYQRLRSAAPVHLRGVAAHFLDRLTPDELRQLDGLLRRVMEGAVP"
     gene            complement(2486..3364)
                     /locus_tag="VFV59_02100"
     CDS             complement(2486..3364)
                     /locus_tag="VFV59_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324650.1"
                     /translation="MPELLVLAVGLVIAGIAVLAPLRGGRVAPDAASELEDARETALL
                     RHRVALEALRDVEADHRSGTLTDAAYEAALVEAEARAAATRAAVDSAPVLEPAPGDGR
                     GPRLGAIAAAVIGAVLLAAAAIPGSGVANRTVVNQSLADAQAAETARQARIAELLDAL
                     RSDTANAEALSDLADAYLEGTSQDDLVRAAVALRLLIELEPDRADAYERIVAAYLRAG
                     DYANARAATDAYAERDTADPVEVAFLDGLIALRGENDPDRAVEAFDRFLELAPDDPRA
                     GMVRSLRDEAAAETEG"
     gene            complement(3357..3815)
                     /locus_tag="VFV59_02105"
     CDS             complement(3357..3815)
                     /locus_tag="VFV59_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEU5324651.1"
                     /translation="MRGWIALVGVALLAALVAVVLLRPQPEASTAERARQVASGLRCP
                     DCQGLSVADSPTAAAQEIRRQVDEMVAAGATEQEIRDHFVARYGEWILLSPSTPAWWL
                     IPFVVLLVGAGGLGWWLLRGRSDRSPDANATPLPDAERRRLHDEAEALDA"
     gene            complement(3812..4336)
                     /locus_tag="VFV59_02110"
     CDS             complement(3812..4336)
                     /locus_tag="VFV59_02110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU5324652.1"
                     /translation="MRTLRWLLIPAIVLPLGWLLLTGFGRDPREIATPLIGREAPAWT
                     YATLDGETLSSADLTGRPYVLNFWASWCIPACVEEHPVLADAAERLGDRVAFVGVLYQ
                     DSPTDAEAFLARYGDAGYPHLVDAAALAVDYGVTGPPETWFVDAEGVVRARKWGPLTD
                     ATMREYLAAIGVSG"
     gene            complement(4340..6349)
                     /locus_tag="VFV59_02115"
     CDS             complement(4340..6349)
                     /locus_tag="VFV59_02115"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEU5324653.1"
                     /translation="MIPTIGHTAVLIGLALTVYAAVAFVLGARGGDQRLVGSARRAVI
                     GSFVAAALGCAAMVISLLTHDFSVLYVARNNATTTPPFISAISLWAALEGSILFWALL
                     ATGWASLVLWRQRNRHRQLMPWVGATLATVNAFFFAVMTWPGNPFARTTPVATEGLGP
                     NALLQNHPFMALHPPLLYLGYTGLAVPFAFGVAALVTKRLDEEWLRIVRRWTLVPWTF
                     LSLGIVAGAWWSYEVLGWGGYWAWDPVENAALLPWLTATAFVHSAIVAERRNGLRIWT
                     SALVIATFVLTLVGTFLTRSGVVASVHSFTQSAIGPWFLVAIVAALGGALTLLVWRLP
                     ELTGGGRPSSTISRESAFLLNNVLFLGVTFAVLFGTLLPLLVAATSGETISVGAPWYN
                     TVTVPMFVALLFLMGIGPALPWGAASWRTVRDRFAVPVAAGAVVAVVAFGIGLREPGS
                     LGILALAVIVAGVMVDEVVRGARARARGRGEDPATATWRLATRNRRRYGGYTVHLGVL
                     VMAIAIAVSSGLSTDRTVTLRPGESAEVGAYTVTHDRLVVAPLPDDARVIETAAEVRY
                     DGPQSGTLRTALRDYPNSQAAIATPAVRTSLGEDLYVTLLASDADSGAVTLHVFINPF
                     VVWIWIGGAIVGMGAVFAAWPERRLRTATASEPMPVPATPSVESA"
     gene            complement(6354..6818)
                     /locus_tag="VFV59_02120"
     CDS             complement(6354..6818)
                     /locus_tag="VFV59_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU5324654.1"
                     /translation="MTVVAPPEPALPPRRRPWGVLVLVAVVLVVVGYLAFSSVGNALV
                     YYLTPTELADRGDAAIGETVRLGGQVKPGSVEGDATDLTFLLTDGDTDITVHSTVAPT
                     RSFREGSGAVVEGRLGDDGVFEADQVIVKHDENYVAPEPGTQPSDHTFDPGE"
     gene            complement(6815..6937)
                     /locus_tag="VFV59_02125"
     CDS             complement(6815..6937)
                     /locus_tag="VFV59_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324655.1"
                     /translation="MTDAGYVIAAYAVILGGLATYTLTLWRRLRAARDRRPEDR"
     gene            complement(6934..7692)
                     /gene="ccsA"
                     /locus_tag="VFV59_02130"
     CDS             complement(6934..7692)
                     /gene="ccsA"
                     /locus_tag="VFV59_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5324656.1"
                     /translation="MKTRILGFVAAAAIAAMAIVSLFVVPADLNQGDAQRVMYPHVAS
                     AWLGYLSFGVTAVAGILWVWKRDLRFDAVALAGAEIGVMFTAFAIWGGMMWGRPVWGV
                     FWQWEDPRLTTTALLLALYVGYLLFRRLTDDPARRATRAAIVGIVAAVDIPIVHFSVE
                     WWRGLHQGATIGSPSRMLNPAAPMVFVGTLLGMLAAFTLAWVYLMIRRYQLARVQMAR
                     EEAVRLERLAAARAVAPRASTPAPRTPESTEAAR"
     gene            complement(7692..8381)
                     /locus_tag="VFV59_02135"
     CDS             complement(7692..8381)
                     /locus_tag="VFV59_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU5324657.1"
                     /translation="MSGLRSEARVALAIARKDALAELRGRHATVSTLFFAAIVLLLFG
                     FALGPDSARLAAAAPGLLWLAIVFAGILAVSRLHLLETDDEALEQLALYPVARRAIYA
                     GKALGGLAVMLVLGLLVLGAVGVLFAVDIGGALLPLLLTVILGAIGIAAVGTFYAGVT
                     VRLRAREVMLPLLMLPVLAPLLLGAVKATTAALTGDPFGELASWLQLLVAFDVIMVAA
                     GAATYGYLLED"
     gene            complement(8378..9148)
                     /gene="ccmA"
                     /locus_tag="VFV59_02140"
     CDS             complement(8378..9148)
                     /gene="ccmA"
                     /locus_tag="VFV59_02140"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEU5324658.1"
                     /translation="MPQTIYVSPRSLGAFDERPPAVVTADLARLFAGSPALAGVSLRV
                     DAGATVALLGPNGAGKTTLLRLLATVIRPSYGTARVDGLDIGREPELVRARVAYLSHA
                     TGLYDDLTARENLAFAAEMLATPDARHRVTRALEDVGLAGRADDRVRGFSAGMRRRLA
                     LGRILLGSASLVLLDEPYAALDADGMALVDQLLTAWRDAGVTVLLTSHAPERLAVTID
                     GSVVLDRGVVAETRGNGVAELPSALDVPSATPARQVVS"
     gene            9283..9735
                     /locus_tag="VFV59_02145"
     CDS             9283..9735
                     /locus_tag="VFV59_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5324659.1"
                     /translation="MHRHPTAAAVPVLILAFVLAACGQGTAPSSAAATVRATPSASAD
                     ASASIEPSASADASGDAAEVRVRLDGFAFDPEELTVAAGTKVTFLNTDPATHTVTEGT
                     DGVAADGAFINDELAANQATSHTFDEPGTYHITCLKHPTMNMTITVTG"
     gene            9801..10175
                     /locus_tag="VFV59_02150"
     CDS             9801..10175
                     /locus_tag="VFV59_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5324660.1"
                     /translation="MDAGLSETTPTSRSRAARRAAAQRAECAATAALNVLGQKWVLRI
                     VRALGERKQRFCELQDALGGANSATLSQRLKLLEDEGLIERRAISTVPPWVEYSLTDK
                     GTDLRSAIAGIDRWAERWAANQ"
     gene            10172..10660
                     /locus_tag="VFV59_02155"
     CDS             10172..10660
                     /locus_tag="VFV59_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5324661.1"
                     /translation="MSHPVAEERPVEHGDALNMIRTHIAEAPGPEAAAQRVVDLLNER
                     FGHYDWVGVYWVDGDHLVLGPWIGPEATEHTRIAIGTGVCGAAAASGQTEIVPDVNAD
                     PRYLSCFASTRSEIVVPIFHEGAVVGEIDIDGSDLDAFDTTDARFLEEVAALLAPLRP
                     GA"
     gene            10685..11221
                     /locus_tag="VFV59_02160"
     CDS             10685..11221
                     /locus_tag="VFV59_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5324662.1"
                     /translation="MSSDPGPFEQVYDEHVVGIYRFIYARVGNHPDAEDLTAQVFVRA
                     VEQLDTSRDPGQIAAWLYRVAHNAIADHWRAFYRLPVIGADHVAPGWEPADPAPTPVA
                     GATDAADTAHDRARDRVRSLLDRLPEPYRRVLELRFLQRLSVAETARAMGITDGNARV
                     LQYRALRRAAVLGEVADG"
     gene            11214..12032
                     /locus_tag="VFV59_02165"
     CDS             11214..12032
                     /locus_tag="VFV59_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEU5324663.1"
                     /translation="MADRDRDARNDQDAAERFDRYLESLLGDGRPSPEAVGDRDEAEM
                     ARTAAELAALARPEEADPDPAFVEQLRLRMREADAGIAHVRTPPPVREPRTPEPPTQL
                     RWRVTRRDVLRAGMGAAAGLAAGAVGLSLIGDRREGQNGLWRDTPLVAGEGFWAEVAT
                     LADLPAGAALRFSTAAFDGYVVNDDGEVRALSSVCTHMQCTLHYRPDWQDLRCPCHGA
                     SFDLRGRLANGPAAWGDRGGYPGDARAYPVDLPDLARPRVKVEDGTIYVWTAQV"
     gene            complement(12042..12272)
                     /locus_tag="VFV59_02170"
     CDS             complement(12042..12272)
                     /locus_tag="VFV59_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324664.1"
                     /translation="MPDRGLKLPDVSQPHKYEHRPPRRPSHLPRRLTPPPERGEPPDL
                     SLFLYAGVVLLVILMILAVPYLIGYLFEVVRF"
     gene            complement(12280..14679)
                     /locus_tag="VFV59_02175"
     CDS             complement(12280..14679)
                     /locus_tag="VFV59_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEU5324665.1"
                     /translation="MTAVRPASHDVDALFDRASEDFAAWELVKDVIDEAIDLTLNYRQ
                     SGHPGGSRSKVHLFLSLLLSGAMRWDIRRPWLPFADRFVLSAGHTVPLVYTTLAVLNE
                     TLRARHQRDGDERFAFPDDGRWALTWEDLLRLRRRGGLAGHAEMEGKTLFLKFNTGPS
                     GHGMPPAAGEATALRLAGAGEVKVFAVEGEGGLTPGASHETRNSAWGLGLSNLVFLID
                     WNDYGIDDVAIHEVVHGTPPEWFQPYGWRVTGTEQGSEWEPVTRAVLEAARGDNAAGV
                     PSMAWFKTRKGRGYGTYDNKSHGTPHPMNSAPFWEVRKAFMARHGVEYVGVDEAAPTD
                     ADEREAQARRNFEIAFGVLRENAEVVDRITDRLLELAASVPERVEGFTLGGHGAEIFD
                     DPRFTDVSAYPESIWKKPGEKAPNRAALAAWGAWVNATARAEYDRPLVIACSADLAES
                     TNIAGFAKDWEGLPGWGWYNRDTNPRGTLLPQEITEFTNAGISVGIATVNLADDPFHR
                     FNGFWAACSTYGSFSYLKYGPMRLFSQLAQDSNLKLGKVLWVAGHSGPETAEDSRTHF
                     GIFATGVTQLFPEGHVIDLHPWEYNEVPVLLAAALATDVPIVALHLTRPAIEIPDREG
                     LGMASHFEAARGAYVLRQPKPGSPCAGTVYVQGTMTTANVVKVLPELDRRDLNVRIVA
                     AVSPQLFRMQDAAYRDEICSPADRWDGMAITNRAYKLMRDWVDGPISEEYSLSSDWDN
                     RWRTGGTVDEVIDEAHLGPDHIVAAIERYVADRDVRMERLRGLVDAVERDHRGVREGE
                     G"
     gene            complement(14676..15884)
                     /locus_tag="VFV59_02180"
     CDS             complement(14676..15884)
                     /locus_tag="VFV59_02180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5324666.1"
                     /translation="MRLARRMDRIGTETAFEAAARARALEATGRDVIHLEIGEPDFDT
                     PRNVSEAAARALLDEGATHYTNSTGIAPLREAIAADVKRWKGIEASPDQVVVTPGAKP
                     IMFYAMLALIDEGDEVIYPNPGFPIYESMARYVGGTAVPAPLREENEFRMDVEEVASL
                     ITPRTRLLVFNTPHNPTGSVLTDADIRRLAELAVEHDLVVLADEIYGRLQYEGELLSI
                     ATLPGMAERTITLDGFSKTFAMTGWRLGYGILPEWLVPSFSRLVINSVSCTNAFAQFG
                     AIEALTGPQEAADAMRAEFIARRQLMVDGLNAIPGVSCVMPHGAFYTFPNVSSFGRSS
                     AEIADHLLYDAGVCGLAGTAFGKYGEGYLRFSYANSRENLERALERVGESLAKLERTG
                     ELATAGAARS"
     gene            16040..16456
                     /gene="erpA"
                     /locus_tag="VFV59_02185"
     CDS             16040..16456
                     /gene="erpA"
                     /locus_tag="VFV59_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU5324667.1"
                     /translation="MSETPREPISMLGGAPSGDPLVTVTEAAARKVVELRDREGKADA
                     RLRLFVKSGGCSGFSYGLAFDDRVNDGDLVEDHDGVPVVIDQFSQQYVNGAEIDYVDS
                     LMGSGFAINNPNAVSSCACGSSFNTDGSPAKAGGCH"
     gene            16539..17276
                     /locus_tag="VFV59_02190"
     CDS             16539..17276
                     /locus_tag="VFV59_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324668.1"
                     /translation="MSSTFPTEPSGDPAERIRREFAAAWGGIGAAWGVAPSTAMVQGY
                     LLVSDGPLSEPEVRRALGMSHRAASLALTQCEEWGLIQRSAAPRRSGNRGPAAVAWTV
                     VGDHWEWFRRVAAARKQRETDPVVPVIERCTLLAREAAASDAAGGELTELAMRLDALL
                     AFVTRFDRGVEVFVRGDATAIEQLFGALDRLSPEAADRLWRLLGELGSDEIARALGAL
                     AKLSPDATRRLVGLADQPVLQKLIGLR"
     gene            complement(17260..17643)
                     /locus_tag="VFV59_02195"
     CDS             complement(17260..17643)
                     /locus_tag="VFV59_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324669.1"
                     /translation="MALPACAAPPSDEGGMGLPQQLPGETYGGARADLAGTLGVAENG
                     CVYLETEGVERLLIFPPGSALSEPARLSDGTELHEGDALGGRGVVVPTETLPGGRDGY
                     WASVSGYCAGDVPEAAVFDEISAGR"
     gene            complement(17681..19135)
                     /locus_tag="VFV59_02200"
     CDS             complement(17681..19135)
                     /locus_tag="VFV59_02200"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU5324670.1"
                     /translation="MTIATVADLRERLAGVATITETGLEVHDESRLRGDLMDELAFAA
                     ALADDGDLREAARWVIWSASQALGCGSASIHELYMARGRGEIEPTAFTVPAINVRATT
                     FLTARQAFAAALERDAGTVIFEIAKSEMAYTEQRPAEYAAVILAAALREGWRTPVFLQ
                     GDHFQFNRTKWGTDPSAELDGLKDLTREAVAAGFLNIDIDSSTLVDLDQPTVPEQQKV
                     NADNSAELTRLIRELQPDGVTISIGGEIGEVGKANSTEEELRAYLDEYRRALGDDGIV
                     GVSKVSIQTGTSHGGVPLPGGGVAEVKLDFGTLDRLGRVAREEYGLAGCVQHGASTLP
                     EEAFHHFPEHGTAEVHLATGFQNILYDEPGLPAELRAEMLAWCVANCADERKDGETDD
                     QFLYKTRKKAIGPFKRQLWTLPEDAAAQVAANLRAKFGQLFDELKIAGNRDLVERHIT
                     PVRVAKPLPASLGGGERRAVAAGAHVFEDDGSGE"
     gene            complement(19151..19357)
                     /locus_tag="VFV59_02205"
     CDS             complement(19151..19357)
                     /locus_tag="VFV59_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324671.1"
                     /translation="MRPFAVGVVALAALLYVAALTAVRWWTRGLQRRGVADGRAARDW
                     YLLAAALGLLASPLLAFTLASGLR"
     gene            complement(19486..20067)
                     /locus_tag="VFV59_02210"
     CDS             complement(19486..20067)
                     /locus_tag="VFV59_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324672.1"
                     /translation="MSSYPAYRPRGPMSRLLGGADDFMNWMLYGYETWLVAVLKGVPL
                     FLFMYFIIFYLPNYVYYLITVELPFLKFSDDVGFLVANGVGMTNLALVVVLALLVQAA
                     RGRRGFGWSAIRIFVLLNYLLAVLMIIPIMAFNLAGGSFWPPRFPLQAIGFGTIAAGL
                     GAAACVYLWFEFRRITGREAAEAARQSAALARR"
     gene            complement(20071..>20457)
                     /locus_tag="VFV59_02215"
     CDS             complement(20071..>20457)
                     /locus_tag="VFV59_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5324673.1"
                     /translation="VESWAAEAANQPPEDVPNTIEAGAEVYASAGCASCHGPDLAGTA
                     AGPDITGIKDDVITEGLPVTPSGVDQMIADYQDDPRMFLEKWIRDSAGNYNDGQPTGM
                     PPHPESSLNASSLQALISFLLDDTGE"
BASE COUNT         2978 a         7318 c         7393 g         2768 t
ORIGIN      
        1 acgcgcccgt tcggcgcgtc gcggtccgtc agcaggtaga gggtgccgtc gacggcgccg
       61 atcggctcgt agcgcgcatc ggggacgtcg agcaggcgcc ggacctcgat cgagtccacc
      121 ccgccggcga ggtcggccac ccacaggcgg ttctccggat cggtcccgcg ccagacgctg
      181 accacgagca ggcgaccctc gtcggtgacc atcggctcga agccccagtc cggatgctcg
      241 gggacggaga gcaccaccac gtcgtcggcc gcatcggtgc cgagccggtg gtagcacagc
      301 tccatctgga ggttcggcgc gtcgtacgcc gcgtcgggcg gcggctccgg gtagcgaccg
      361 tagacgaagc cctcgccgtc agcggtccag gcggcggagg cgaacttgct ccacgtgacc
      421 cggtccgcca ggtcgtcgcc cgtggccacc tcgcggacgg accaggtgcg ccagtcggac
      481 ccggcgtcgc tgatggcgta ggcgagccgc tcaccgtcct cgctgatggc catggccgac
      541 agcgcccgcg ttccctgctg cgagagaccg ttcggatcca gcagcacgcg cccgggatcg
      601 tcgggcgcat ccgagaccca gaggacgtcc tggtcctgga ggccggtgtt gcggagctgg
      661 aaccagcggt cgccacggtg gatcggcgca ccggcccgtg ggaagtccca cagctcggcg
      721 aggcgggcac ggatctcacc gcgcgcggcc agcccggaga gcacccgttc cgtcaggtcg
      781 ttctccgccg cgatccaccg gcgcgtctca ggcgcatcgg tgtcttcgag cgggcggtag
      841 ggatcggcca cggccacgcc gtggtagtcg tccacctggt cgctggtcgg cgccggtggg
      901 tagctcggct tcggcatccg cgccatgcta ggcgttcacg gccgcgcgct cagcgtggaa
      961 gggtctccat gtagagatcg tcggggtagc agtagcccca ctcctccccg ggctcgtgcg
     1021 accggatgat cgggtggccg ctggcgtgga cgtgcgcggt ggcgtgccgg ttcggcgact
     1081 ggtcgcagca gccgacgtgg ccgcaggtca tgcacagccg caggtggacc cagcccgagc
     1141 cgatgcgcag gcagtcctcg cagccgtcgg cgctgggctc gacgtcgtgg atctggtcga
     1201 ggtgcgtgca ggcctcgttc atcgggccat ggtacgcgcg aggggccggg tgaatccggc
     1261 ccctcgggtg tcgtcgcgtc ggttgcggat caggcgctgg cgcgggcggc ctcgcgctcg
     1321 tccatctcgg actccgcctc ggcctcggcg gtcacctcgg cggcggagga catggcctcg
     1381 aggtcgatct cgatcttgat gtccttgctg accaggaggc cgccctgctc gagcgccacg
     1441 ttccaagtca ggccgaagtc ctcgcggttg atcttcgtcg tcgcgctgaa gccggcgctc
     1501 ttgccgccct gcatgttcgt gacggtgccg gcgtattccg ccttgagcac cgccgggcgg
     1561 gtcgtgccgc gaatggtgag atcaccgcgg atctcatact cgtcaccgcc ccgcggcacg
     1621 atctcggtgc tctggaacgt gatgtccggg tggttcgcga cgtccaggaa gtccgccgaa
     1681 cgcaggtggt cgtcgcgcat ctgctggtcg gtgtcgatgg acgccgcgtc cagcacgacg
     1741 cgcacgcgcg agtcgaccag gttgtccggg tcgaagtcca ggtcgaagtc gcggatcttc
     1801 atgctgccgc gcacggtcgt caccatcatg tgcttggcgc tgaaggtgac ggacgagtgg
     1861 gcggagtcga tggtccaggt catgaggtga gtgctctcca tggtctgcgg gttcgcggcg
     1921 ctcgcggggc tcgcggagtt ccgcggtgct tcgtgatatg attgacaccg caatcatatg
     1981 ccaccggtgt ttgttgtgca acaacgaagt tgtgccctat gactattgat gccccgcagg
     2041 tttcgcgcca cgacccccgc atctcggcct ggcggacgtt cctcgaaacg cacgcgcggg
     2101 tcgtccgcga gttggaacag gagctccagg cggaggccga tctgccgctg acccactacg
     2161 acgtgctcgt gcagctctcg ggagccgatg cgcgccgcct gcgcatgagc gagctcgccg
     2221 atcgcctgct gctgtcgcgg tccggcgtca cccggctcgt cgaccggctg gtcgccgatg
     2281 gcctggtcga gcgtgtctcg tgcgactccg accgccgcgg cgcctgggca tccctgaccg
     2341 atgcgggcta tcagcgcctt cgatccgccg cgccggtcca cctgcgcggc gtggccgccc
     2401 acttcctgga ccggctgacc cccgacgagc tccgtcagct ggacgggctg ctccggcgcg
     2461 tgatggaggg agcggtcccc tagcgctagc cctcggtttc ggcggcggcc tcgtcccgga
     2521 gcgaacgcac catgccggcc cgcggatcgt ccggcgccag ctcgaggaac cgatcgaacg
     2581 cctcgaccgc ccggtcgggg tcgttctcgc cgcgcagggc gatgagcccg tcgaggaagg
     2641 cgacctcgac cggatcggcg gtgtcgcgct ccgcgtacgc gtcggtcgcg gcgcgcgcgt
     2701 tggcgtagtc acccgctcgc aggtacgccg ccacgatccg ctcgtaggcg tccgcccggt
     2761 ccggctcgag ctcgatcagc agccgcagcg cgaccgcggc gcgcaccagg tcgtcctggc
     2821 tcgtgccctc caggtacgca tcggccaggt ccgacagcgc ctccgcattc gcggtgtcgc
     2881 tgcgcagggc gtcgagcagc tcggcgatgc gcgcctggcg tgcggtctcg gcggcctggg
     2941 catcggccag cgactggttg acgacggtcc ggttggcgac gccgctcccc gggatggccg
     3001 ctgcggcaag gagcacggcg ccgatgacgg ccgccgcgat cgcgccgagc cgcggcccac
     3061 gcccgtcgcc gggcgccggc tcgaggacgg gagccgagtc cacggccgcg cgggtcgcgg
     3121 cggctcgcgc ctccgcctcg acaagggccg cctcgtaggc ggcatcggtc agcgtgcccg
     3181 agcggtgatc agcctccacg tcgcgcagcg cctcgagcgc cacgcggtgg cggagcaggg
     3241 ccgtctcgcg cgcgtcctcc agctccgagg ccgcgtcggg cgccacgcgg ccgccacgca
     3301 gcggtgccag gaccgcgatg ccggcgatca cgagcccgac ggcaagcacc agcagctcag
     3361 gcatccagcg cctccgcctc gtcgtgcagc cgacggcgct ccgcgtcggg cagcggggtc
     3421 gcattggcgt ccggcgagcg gtcgctgcgc ccgcggagca gccaccagcc caggccgccg
     3481 gcgccgacca gcagcacgac gaacgggatg agccaccacg ccggcgtcga cggcgacagc
     3541 aggatccact cgccgtagcg cgccacgaag tggtcgcgga tctcctgctc ggtggcgccg
     3601 gcggcgacca tctcgtcgac ctggcgccgg atctcctgcg cggcggcggt cggcgagtcg
     3661 gcgaccgaga ggccctggca gtccgggcat cgcaggccgg aggcgacctg gcgcgctcgc
     3721 tccgcggtgc tcgcctcggg ttgcggtcgc agcaggacga cggcgaccag cgccgccagc
     3781 agcgcgaccc ccaccagggc gatccagccc ctcatccgct caccccgatc gccgccaggt
     3841 actcgcgcat ggtcgcgtcg gtcagcggac cccacttccg ggcgcgcacg acgccctcgg
     3901 cgtcgacgaa ccacgtctcc ggcgggccgg tcaccccgta gtcgacggcc agcgccgctg
     3961 cgtcgaccag gtgcgggtac cccgcgtcgc cgtagcgggc caggaaggcc tccgcatcgg
     4021 tcgggctgtc ctggtacagg accccgacga aggcaacgcg gtcgccgagc cgctcggcgg
     4081 catcggccag gaccgggtgc tcctcgacgc aggcggggat gcaccagctg gcccagaagt
     4141 tcagcacgta cggacggccc gtcagatcgg ccgagctcag ggtctcgcca tcgagggtcg
     4201 cgtaggtcca cgcgggcgcc tcccgtccga tgagcggcgt cgcgatctcg cgcggatcgc
     4261 gtccgaagcc ggtgagcagc agccagccga gcggcaggac gatggccggg atcagcagcc
     4321 agcgcagcgt gcgcatgggt caggcgctct cgaccgacgg cgtggccggc accggcatcg
     4381 gctccgaggc ggtcgccgtg cgcagccgcc gctccggcca ggccgcgaag acggcgccca
     4441 tgccgacgat ggcccctccg atccagatcc agaccacgaa cgggttgatg aagacgtgca
     4501 gcgtgaccgc gcccgagtcg gcgtccgagg ccagcagagt cacgtacagg tcctcgccca
     4561 gcgacgtgcg cactgcgggg gtggcaatgg cggcctggga gttcgggtag tcgcgcaggg
     4621 cggtgcgcag cgtgcccgac tgcggaccgt cgtagcgcac ctcggcggcg gtctcgatca
     4681 cacgcgcgtc gtcgggcagc ggcgcgacca ccaggcggtc gtgggtgacg gtgtacgcac
     4741 cgacctccgc gctctcgccg gggcgcagcg tcacggtgcg atcggtcgag aggccgctgg
     4801 agacggcgat ggcgatcgcc atgacgagga cgcccaggtg gacggtgtac ccgccgtagc
     4861 ggcgccggtt gcgcgtcgcc aggcgccacg tggcggtggc cggatcctcg ccacggcccc
     4921 gcgcccgagc tcgcgccccg cgcaccacct cgtcgaccat caccccggcc acgatcaccg
     4981 ccagcgccag gatgccgagc gacccgggct cgcgcaggcc gatgccgaag gccacgacgg
     5041 cgaccaccgc gccggccgcc accggaacgg cgaaccggtc gcgcaccgtc cgccacgagg
     5101 cggcacccca cggcagcgcc ggaccgatgc ccatgaggaa cagcagcgcg acgaacatcg
     5161 ggacggtcac cgtgttgtac cagggcgcgc cgacgctgat cgtctctccg ctggtggccg
     5221 ccacgaggag cggcagcagc gtcccgaaca ggacggcgaa ggtgacgccc aggaagagga
     5281 cgttgttgag caggaaggcc gactcgcggc tgatcgtgga cgagggccgg ccgccgccgg
     5341 tgagctcggg caggcgccag accagcagcg tcagcgcacc accgagcgcc gccacgatcg
     5401 ccaccaggaa ccacggcccg atggccgact gggtgaacga gtggacgctg gccacgacgc
     5461 cggatcgggt gaggaacgtg ccgaccaggg tgagcacgaa ggtggcgatc accagggcgc
     5521 tggtccagat gcgcagcccg ttgcgccgct ccgccacgat cgccgagtgc acgaaggcgg
     5581 tggccgtcag ccacggcagc agcgccgcgt tctcgaccgg gtcccacgcc cagtagccgc
     5641 cccagccgag cacctcgtag ctccaccacg ccccggccac gatgcccagg ctcaggaagg
     5701 tccacggcac gagcgtccag cgccgcacga tgcgcagcca ctcctcgtcc aggcgcttcg
     5761 tcaccagcgc cgcgacgccg aacgcgaacg ggaccgccag cccggtgtag ccgaggtaca
     5821 gcagcggcgg atgcagcgcc atgaacgggt ggttctgcag gagcgcgttg ggcccgaggc
     5881 cctcggtcgc gaccggcgtg gtgcgcgcga aggggttgcc gggccaggtc atgaccgcga
     5941 agaagaaggc gttgacggtc gccagcgttg ccccgaccca gggcatcagc tgccgatgcc
     6001 gattccgctg ccgccacagc accagcgacg cccagccggt ggcgaggagg gcccagaaca
     6061 ggatcgagcc ctccagcgcg gcccataggc tgatggcgct gatgaacggc ggcgtggtgg
     6121 tcgcgttgtt gcgggccacg tacagcacgc tgaagtcgtg cgtgagcagc gagatgacca
     6181 tcgcggcgca gcccagggcg gccgccacga aggagccgat gacggcgcgc cgtgcgctgc
     6241 cgaccagccg ctggtcgccg ccgcgcgccc cgagcacgaa ggcgacggcg gcgtacaccg
     6301 tcagcgccag gccgatgagc acggccgtgt ggccgatggt cgggatcacc cggtcactcc
     6361 ccggggtcga acgtgtgatc cgacggctgc gtcccgggct ccggtgcgac gtagttctcg
     6421 tcgtgcttca cgatgacctg gtccgcctcg aagaccccgt cgtcgccgag ccggccctcg
     6481 accacggccc cggagccctc gcggaaggag cgcgtcggtg caaccgtcga gtggacggtg
     6541 atgtcggtgt cgccgtcggt gaggaggaat gtgaggtcgg tcgcgtcccc ctcgacgctt
     6601 cccggtttga cctgcccgcc cagccggacc gtctccccga tggccgcatc cccgcggtcc
     6661 gccagctcgg tcggcgtcag gtagtacacg agcgcgttgc cgacgctgct gaaggcgagg
     6721 tacccgacga ccaccaggac gacggcgacc agcacgagca cgccccacgg tcgacggcgc
     6781 ggcgggagag cgggctcggg tggcgcgacg accgtcatcg gtcctcgggg cgtcggtcgc
     6841 gggcggcgcg cagccggcgc cacagggtga gcgtgtaggt cgcgagcccg ccgaggatga
     6901 cggcgtaggc ggcgatgacg tagcccgcat cggtcaccgg gcggcctccg tcgactccgg
     6961 cgtccgcggg gcaggcgtgc tcgccctcgg agcgaccgcg cgagccgctg ccagccgttc
     7021 gagccgcacc gcctcctccc gcgccatctg cacgcgcgcc agctggtagc gccggatcat
     7081 gaggtagacc caggccagcg tgaaggcggc cagcatgccg agcagcgtgc cgacgaagac
     7141 catcggagcg gccgggttga gcatgcggga cggcgagccg atcgtcgcgc cctggtgcag
     7201 gccacgccac cactcgacgc tgaagtggac gatcgggatg tcgacggcgg ccacgatgcc
     7261 gacgatggcg gcgcgcgtcg cgcgacgggc gggatcatcg gtcaggcgcc ggaagagcag
     7321 gtagccgacg tacagcgcca ggagcagggc ggtggtggtg aggcgcgggt cctcccactg
     7381 ccagaagacg ccccacaccg gcctgcccca catcatcccg ccccagatcg cgaaggcggt
     7441 gaacatgacc ccgatctcgg ccccagccag cgcgacggca tcgaagcgca ggtcgcgctt
     7501 ccacacccag aggatccccg ccaccgccgt gacgccgaac gagagatagc cgagccacgc
     7561 ggatgcgacg tgcgggtaca tgactcgctg cgcgtcaccc tggttcaggt cggccggcac
     7621 gacgaacagc gacacgatcg ccatggcggc gatggcggcg gcggccacga aaccgaggat
     7681 gcgggtcttc atcagtcctc cagcaggtag ccgtaggtcg cggccccggc ggcgaccatg
     7741 atgacgtcga acgcgacgag gagctggagc cagctcgcca gctcgccgaa cgggtccccg
     7801 gtgagtgccg cggtcgtcgc cttgaccgcg ccgagcagca gtggcgccag caccggcagc
     7861 atgagcagcg ggagcatcac ctcgcgggcg cggagccgga cggtgacgcc ggcgtagaac
     7921 gtcccgaccg ccgcgatgcc gatggccccc aggatcacgg tcagcagcag cggcagcagg
     7981 gccccgccga tgtcgacggc gaacagcacg ccgaccgcgc cgaggaccag caggccaagg
     8041 acgagcatca ccgccaggcc gccgagcgcc ttgcccgcgt agatcgcgcg gcgcgccacc
     8101 gggtagaggg cgagctgctc gagcgcctcg tcatcggtct cgagaaggtg caggcggctc
     8161 acggccagga tcccggcgaa gacgatcgcc agccacagca gccccggcgc cgcggcggcg
     8221 aggcgcgcgg agtccggacc gagcgcgaag ccgaacagca gcagcacgat ggccgcgaag
     8281 aagagggtgc tgacggtcgc gtggcggccg cgaagctcgg ccaacgcatc cttgcgggcg
     8341 atggcgagcg ccacccgcgc ctccgaacgc aggccgctca cgagaccacc tggcgggcgg
     8401 gcgtggccga cgggacgtcg agcgccgagg gaagctccgc cacgccgttg ccgcgcgtct
     8461 ccgccaccac cccgcggtcg agcaccactg acccgtcgat ggtcacggcc agccgctccg
     8521 gtgcgtgcga ggtgagcagc accgtgacgc cggcgtcgcg ccaggcggtg agcagctggt
     8581 ccaccagcgc catgccgtcc gcatccaggg cggcgtacgg ctcgtcgagc agcacgagcg
     8641 aggcgctgcc gagcaggatc cgcccgagcg ccaggcggcg gcgcatgccg gccgagaagc
     8701 cgcggacccg atcgtcggcg cggccggcca ggcccacgtc ctccagcgcg cgggtcaccc
     8761 gatgccgggc gtccggcgtg gccagcatct cggccgcgaa cgccaggttc tcgcgggcgg
     8821 tcaggtcgtc atacaggccc gtggcgtggg agagataggc gacccgcgcc cggaccagct
     8881 ccggctcgcg accgatgtcc agcccgtcga cccgcgccgt cccgtagctc gggcggatga
     8941 cggtcgccag cagccggagc agcgtcgtct tgccggcccc gttcggcccg agcagcgcga
     9001 ccgtggcgcc cgcatcgacg cgcagcgaga caccggccag cgcgggcgag cccgcgaaca
     9061 ggcgtgccag gtcggcggtg acgacggctg gaggacgttc gtcgaaggcc ccgagggagc
     9121 ggggcgagac gtagatggtc tgcggcatgc tcgacgtggc gggggctcga aggtgggcgc
     9181 tctgcactat gcggcccggg caacggagcg tcaaacgcgc cgtaaccggg tccgtatcgg
     9241 tctcgacgga accaccaccc catccatgga gcacccagac cgatgcaccg ccacccgacc
     9301 gccgcagcgg tcccagtcct catccttgcc ttcgtcctcg cggcctgcgg ccagggcacg
     9361 gcgccatcct cggcggccgc gaccgttcgc gcgacgccgt ccgcctcggc ggacgcatcg
     9421 gcgtcaatcg agccatccgc gagcgcggac gcgtccggcg acgccgcgga ggtgcgcgtg
     9481 cgcctcgatg gcttcgcctt cgatccggag gagctgacgg tcgcggccgg cacgaaggtc
     9541 accttcctca acaccgaccc cgccacgcat accgtgacgg aagggacgga cggcgtcgcc
     9601 gcggacggcg cgttcatcaa cgacgagctg gcggccaacc aggcgaccag ccacacgttc
     9661 gacgaaccgg ggacgtatca catcacctgc ctcaagcacc cgaccatgaa catgacgatc
     9721 acggtcaccg gctgagcgga tcgggtggtg accttcgccg gttgatgggc cggggggtcg
     9781 gtgctacggt tcgcgtcctg atggacgccg gcctgagcga gaccaccccg accagccgtt
     9841 cgcgcgccgc tcgtcgcgcc gccgcgcagc gggcggagtg cgccgcgacg gccgcgctga
     9901 acgtgctggg ccagaagtgg gtgctgcgca tcgtgcgtgc cctcggcgag cggaagcaac
     9961 gcttctgcga gctccaggac gcgctcggcg gcgccaactc cgcaacgctc tcgcagcggt
    10021 tgaagctgct cgaggacgag ggactcatcg agcgacgcgc catcagcacc gtcccaccct
    10081 gggtggagta ctcgctgacg gacaagggca ccgacctgcg ctcggcgatc gccgggatcg
    10141 accgctgggc ggagcgctgg gcggcgaacc agtgagccac cccgtcgccg aggagcggcc
    10201 cgtcgagcac ggcgacgccc tcaacatgat ccggacccac atcgccgaag cgcccggtcc
    10261 cgaggccgcc gcccagcgcg tcgtcgacct gctgaacgag cggttcggcc actatgactg
    10321 ggtcggcgtc tactgggtcg acggcgacca cctcgtgctc ggcccgtgga tcggccccga
    10381 ggcgaccgag cacacccgca tcgcgatcgg caccggcgtc tgcggcgccg cggcggcatc
    10441 gggccagacc gagatcgtcc cggacgtcaa cgccgatccg cgctacctct cgtgcttcgc
    10501 cagcacgcgc agcgagatcg tggtgccgat cttccacgag ggcgcggtgg tcggagagat
    10561 cgacatcgac ggctccgacc tcgacgcctt cgacacgacc gacgcgcgat tcctcgagga
    10621 ggtcgcggca ctcctggcgc cgctccgccc cggagcgtaa cggcccgcgt accggatcgg
    10681 tgacatgtcg agcgatccgg gcccattcga gcaggtgtat gacgagcacg tcgtcggcat
    10741 ctaccggttc atctacgcgc gcgtcggcaa ccacccggac gcggaggacc tgaccgcgca
    10801 ggtcttcgtg cgcgccgtgg agcagctcga cacctcccgt gacccgggcc agatcgccgc
    10861 ctggctctac cgcgtcgccc acaacgccat tgccgaccat tggcgcgcgt tctatcggct
    10921 gccggtgatc ggggccgacc acgtcgcccc cggatgggag ccggcggacc cggcccccac
    10981 ccccgtcgcc ggcgcgaccg atgcggccga taccgcccac gaccgcgcgc gcgaccgcgt
    11041 ccggtcgctg ctcgaccgac tgccggagcc gtaccgccgg gtgctggagc tgcgcttcct
    11101 gcagcgcctc tccgtggccg agacggcgcg cgccatgggc atcaccgacg gcaacgctcg
    11161 cgtcctccag taccgggcgc tgcgacgggc ggccgtgctc ggggaggtgg ccgatggctg
    11221 accgcgaccg cgacgcgcgc aacgaccagg acgcggccga acgcttcgac cgctacctcg
    11281 agtcgctgct gggcgacggg cgaccgtcac cggaggcggt gggcgaccgc gacgaggcgg
    11341 agatggcgcg cacggccgcc gagctggccg cgctggcgcg acccgaggag gcggacccgg
    11401 accccgcgtt cgtcgagcag ctgcggctcc gaatgcgcga ggcggacgca ggcatcgcgc
    11461 acgtccgcac cccgcccccg gtgcgcgagc cacggacgcc ggagccgccc acgcagctgc
    11521 gctggcgcgt cactcggcgc gacgtgctgc gcgccgggat gggtgccgcg gcgggcctgg
    11581 ctgccggggc cgtcggcctc agcctcatcg gtgatcgtcg ggaaggccag aacggcctgt
    11641 ggcgggacac gccgctggtc gcgggtgagg gattctgggc cgaggtcgcg accctggcgg
    11701 acctgccggc cggcgcggcg ctgcgcttct cgacggccgc cttcgacggc tacgtggtca
    11761 acgacgatgg ggaggtgcgg gcgctgtcat cggtgtgcac ccacatgcag tgcacgctgc
    11821 actaccgacc ggactggcag gacctgcgct gcccgtgcca cggcgccagc ttcgacctgc
    11881 gcggtcggct ggcgaacggc ccggccgcct ggggcgatcg cggcggctac ccaggggatg
    11941 cgcgcgcgta cccggtcgac ctccccgacc tcgcgcgccc gagggtcaaa gtcgaggacg
    12001 gcacgatcta cgtctggacc gcccaggtct aggcgctgct gctagaagcg gaccacctcg
    12061 aagaggtagc cgatgaggta cggcaccgcc aggatcatga ggatcaccag gagcacgacc
    12121 ccggcgtaga ggaacagcga aaggtcgggc ggctcgcccc gttccggggg tggggtgagc
    12181 cgccgcggca ggtggctcgg ccgtcgcggc ggccgatgct cgtacttgtg cggctgcgag
    12241 acgtccggca gcttcagacc gcgatcgggc acgcgccggc tatccctccc cctcgcgcac
    12301 gccgcggtga tcgcgctcga cggcgtcgac caggccgcgc agccgctcca tccgcacgtc
    12361 gcggtcggcc acgtagcgct cgatggccgc gacgatgtgg tcggggccga ggtgcgcctc
    12421 gtcgatcacc tcgtcgaccg tgccgccggt ccgccaccgg ttgtcccagt ccgagctcag
    12481 cgagtactcc tcgctgatcg gcccgtcgac ccagtcgcgc atcagcttgt aggcgcggtt
    12541 ggtgatggcc atgccgtccc agcggtccgc gggggagcag atctcgtcgc ggtacgccgc
    12601 gtcctgcatc cggaacagct gcgggctgac ggccgccacg atgcgcacgt tcaggtcccg
    12661 gcggtcaagc tccggcagca ccttcaccac gttcgccgtg gtcatcgtgc cctgcacgta
    12721 gaccgtgccc gcgcacggcg acccgggctt cggctgccgc agcacgtagg cgccgcgcgc
    12781 cgcctcgaag tgcgaggcca tccccaggcc ctcccgatcg gggatctcga tcgccggtcg
    12841 ggtgaggtgc aacgccacga tcggcacgtc ggtggccagg gcggcggcca gcaggaccgg
    12901 cacctcgttg tactcccacg ggtgcaggtc gatgacatgg ccctccggga agagctgcgt
    12961 cacgcccgtc gcgaagatgc cgaagtgcgt ccgcgagtcc tcggcggtct cgggaccgga
    13021 gtggccggcc acccagagca ccttgcccag cttcaggttc gaatcctggg ccagctggct
    13081 gaagaggcgc atcggtccgt acttcaggta gctgaaggac ccgtaggtcg agcaggcggc
    13141 ccagaagccg ttgaaccgat ggaacgggtc atcggccagg ttcaccgtcg cgatgccgac
    13201 gctgatcccg gcgttggtga actccgtgat ctcctgcggc agcagcgtgc cacgcgggtt
    13261 cgtgtcgcgg ttgtaccagc cccagcccgg cagcccctcc cagtccttgg cgaagccggc
    13321 gatgttcgtc gactcggcaa ggtccgcgga gcaggcgatg accagcggcc ggtcgtactc
    13381 ggcgcgggcc gtggcgttga cccacgcgcc ccacgccgcc agcgccgcgc ggttcggggc
    13441 cttctccccg ggcttcttcc atatcgactc gggataggcg gagacgtcgg tgaagcgcgg
    13501 gtcgtcgaag atctccgccc cgtgcccgcc gagcgtaaag ccctcgaccc gctcggggac
    13561 ggaggccgcc agctccagca gccgatcggt gatgcggtcg accacctcgg cgttctcgcg
    13621 cagcacgccg aaggcgatct cgaagttgcg gcgggcctgc gcctcgcgct catccgcatc
    13681 ggtcggggcg gcctcgtcaa cgccgacgta ctcgacgccg tggcgcgcca tgaaggcctt
    13741 gcgcacctcc cagaacggcg cggagttcat cggatgcggc gtgccgtggc tcttgttgtc
    13801 gtaggtcccg tagccgcggc ccttgcgggt cttgaaccac gccatggacg ggacgccggc
    13861 ggcgttgtca ccgcgggcgg cctcgaggac ggcacgcgtc acgggctccc actcggagcc
    13921 ctgctccgtg ccggtcaccc gccagccgta cggctggaac cactcgggcg gggtgccgtg
    13981 cacgacctcg tgaatggcca cgtcgtcgat gccgtagtcg ttccagtcga tgaggaagac
    14041 gaggttcgac agccccaggc cccaggcgct gttgcgcgtc tcgtggctgg cacccggcgt
    14101 caggccgccc tcgccttcga cggcgaagac cttgacctca cccgctccgg ccagccggag
    14161 agcggtcgcc tcgccggcgg cgggcggcat gccgtggccg gacggcccag tgttgaactt
    14221 caggaagagg gtcttgccct ccatctccgc atggccggcg agcccgccac ggcgtcgaag
    14281 ccgaagcagg tcctcccagg tcagtgccca gcgcccgtcg tccgggaagg cgaaccgctc
    14341 gtcaccgtcc cgctgatggc gcgcgcgcag tgtctcgttc agtaccgcga gggtcgtgta
    14401 gaccagcggc accgtgtgac cggcggagag cacgaaccgg tcggcgaagg gcagccacgg
    14461 ccggcggatg tcccagcgca tggcgccgga gaggagcagc gagaggaaga ggtggacctt
    14521 cgagcgcgag ccgccgggat ggcccgactg ccggtagttc agcgtaaggt cgatggcctc
    14581 gtcgatgacg tccttgacca gctcccaggc cgcgaagtcc tcgctggcgc ggtcgaacag
    14641 cgcgtcgacg tcgtgcgacg cgggtcgaac cgcggtcacg agcgggccgc gccggcggtc
    14701 gccagctccc cggtgcgctc gagcttggcc agggactcgc ccacgcgctc cagcgcccgc
    14761 tccaggttct cccgggagtt cgcgtagctg aagcgcaggt acccctcgcc gtacttgccg
    14821 aaggccgtgc cggccaggcc gcagacgccc gcgtcgtaga gcaggtggtc cgcgatctcg
    14881 gcgctggatc ggccgaagga gctgacgttc gggaaggtgt agaaggcgcc gtgcggcatg
    14941 acgcagctca ctcccgggat ggcgttgagt ccgtcgacca tcagctggcg gcgcgcgatg
    15001 aactcggcgc gcatcgcgtc ggcagcctcc tgcgggccgg tcagcgcctc gatggctccg
    15061 aactgggcga aggcgttggt gcacgacacg ctgttgatga ccaggcggct gaaggacggc
    15121 accagccact ccggcaggat cccgtagccg agccgccagc cggtcatggc gaacgtcttg
    15181 ctgaagccgt cgagcgtgat cgtccgctct gccatgccgg gcagcgtcgc gatcgacagc
    15241 agctcgccct cgtactgcag gcggccgtag atctcatcgg ccaggaccac caggtcgtgc
    15301 tcgacggcga gctcggccag gcggcggatg tccgcatcgg tcaggaccga gccggtcggg
    15361 ttgtgcggcg tgttgaagac gaggagcctg gtgcgcggcg tgatcagcga cgcgacctcc
    15421 tcgacgtcca tgcggaactc gttctcctcg cgaaggggag ccggaaccgc cgtgccgccg
    15481 acgtagcggg ccatcgactc gtagatcggg aagccggggt tggggtagat gacctcgtcg
    15541 ccctcgtcga tcagcgccag catggcgtag aacatgatcg gcttggcgcc cggcgtgacg
    15601 acgacctggt ccggcgacgc ctcgatcccc ttccagcgct tcacgtcggc ggcgatggcc
    15661 tcgcgcaggg gcgcgatgcc ggtcgagttg gtgtagtggg tcgccccctc gtccaggagg
    15721 gcgcgggcag cggcttcgct gacgttgcgg ggggtgtcga aatcgggctc gccgatctcc
    15781 agatgaatga cgtcgcgccc ggtcgcctcg agcgcgcgcg cccgggcggc agcctcgaac
    15841 gcggtctctg tgccgatgcg atccatgcgc ctcgcgagtc tcacgccagg ctcctctcaa
    15901 gtgcagaacg aatggagtct ccagtgtacc gatgatcccc cttgccgggc ccggcgggcc
    15961 gcttgctata ctttccaaag cgaacccagc acatgaaccg atgcgcccga ggcgcgagga
    16021 gaccgattca ctcatgacga tgtccgagac cccccgcgag ccgatctcga tgctcggtgg
    16081 cgcgcccagc ggcgacccgc tcgtgacggt gaccgaggcc gcggcccgca aggtggtcga
    16141 gcttcgtgac cgcgagggca aggccgatgc gcgcctgcgc ctcttcgtga agagcggcgg
    16201 ctgctccggc ttcagctacg gcctggcctt cgacgatcgc gtcaacgatg gcgacctcgt
    16261 cgaggatcat gacggcgttc cggtcgtgat cgaccagttc agccagcagt acgtgaacgg
    16321 tgccgagatc gactacgtcg actcgctgat gggctccggc ttcgccatca ataacccgaa
    16381 cgcggtgagc agctgcgcct gcggctcgtc gttcaacacg gacggctccc ccgccaaggc
    16441 gggcggctgc cactgattgc acgcctcctc cgcgaccgaa gccccggctc cgcgccgggg
    16501 cttcgtcgta tgtggggcga cgcccgtatc atgctccggt gagtagcacg tttcccacgg
    16561 agccctccgg cgacccggcc gagcggattc gtcgggagtt cgcggcggcc tggggcggaa
    16621 tcggcgctgc ctggggggtg gccccgtcga cggcgatggt gcagggctac ctgctcgtct
    16681 cggacgggcc gctctccgag ccggaggtcc gccgggccct cggcatgagc caccgcgccg
    16741 ccagcctggc cctgacgcag tgcgaggagt ggggcttgat ccagcgctcc gccgccccac
    16801 gtcgctccgg caaccggggg ccggctgccg tggcatggac cgtcgtcggc gaccactggg
    16861 agtggttccg ccgcgttgcc gcggcgcgga agcagcggga gacggacccc gtcgtcccgg
    16921 tgatcgagcg ctgcacactc ctcgcgcgtg aggcggccgc ttctgacgcg gccggcgggg
    16981 agctgaccga gctggcgatg cggctggacg ccctgctggc gttcgtcacg cgcttcgatc
    17041 gcggcgttga ggtgttcgtc cgcggcgacg cgaccgccat cgagcagctg ttcggtgccc
    17101 tcgaccgcct ctcgccggaa gcggcggacc ggctgtggcg gctgctgggc gagctgggca
    17161 gcgacgagat cgcgcgagcc ctcggtgcgc tggcgaagct ctcgcccgat gcaacccgac
    17221 gcctggtggg cctggccgat cagcccgtgc tgcagaagct catcggcctg cgctgatctc
    17281 gtcgaagacg gccgcctccg gcacgtcgcc cgcgcagtag ccgctcaccg acgcccagta
    17341 gccgtcacgg ccgccgggga gggtctcggt gggcaccacg acgcctcgcc cgcccagcgc
    17401 gtcgccctcg tgcagctcgg tgccgtccga caggcgcgct ggctcggaca gtgccgatcc
    17461 cggcgggaag atcaggagcc gttcgacgcc ctcggtctcg aggtagacgc agccgttctc
    17521 ggccacgccg agcgtccccg ccagatccgc ccgcgcgccg ccgtacgtct cgccgggaag
    17581 ctgctgcggc aggcccatgc cgccctcgtc ggagggcggc gcggcgcacg cgggcagtgc
    17641 caacgccgcg aggaggacga ggacgagccg ggccggcgcc ctactcgcca gagccgtcgt
    17701 cctcgaacac gtgcgcgccg gccgcgacgg cccggcgctc accgcctccc agcgatgcgg
    17761 gcagcggctt cgcgacgcgg accggcgtga tgtggcgctc gacgaggtcg cggttgccgg
    17821 cgatcttcag ctcgtcgaac agctgcccga acttggcccg caggttggcg gcgacctgcg
    17881 cggccgcatc ctcggggagc gtccacagct gccgcttgaa cggaccgatc gccttcttgc
    17941 gtgtcttgta gaggaactgg tcgtcggtct cgccgtcctt gcgctcgtcg gcgcagttgg
    18001 cgacgcacca cgcgagcatc tcggcccgca gctcggcagg caggccgggc tcgtcgtaca
    18061 ggatgttctg gaacccggtg gccaggtgca cctccgcggt gccgtgctcc gggaagtggt
    18121 ggaacgcctc ctcgggcagg gtcgaggcgc cgtgctggac gcacccggcc aggccgtact
    18181 cctcgcgcgc cacgcggccc agccggtcga gggtgccgaa gtcgagcttc acctcggcca
    18241 cgccgccgcc gggcagcggg acgccgccgt gcgaggtgcc ggtctggatg ctgaccttgc
    18301 tgacgccgac gatgccgtcg tcaccaagcg cacggcgata ctcgtcgagg taggcgcgca
    18361 gctcctcctc cgtcgagttc gccttcccga cctccccgat ctcgccgccg atgctgatcg
    18421 tcacgccgtc gggctggagc tcgcggatga ggcgcgtgag ctccgcgctg ttgtcggcgt
    18481 tgaccttctg ctgctccggg acggtcggct ggtcgaggtc gaccagcgtc gagctgtcga
    18541 tgtcgatgtt caggaacccg gcggcgaccg cctcgcgggt caggtccttg aggccgtcga
    18601 gctccgccga cggatcggtt ccccacttcg tgcggttgaa ctggaagtgg tccccctgca
    18661 ggaagaccgg ggtgcgccag ccctcgcgca gcgcggcggc caggatcacc gcggcgtact
    18721 cggccggacg ctgctcggtg taggccatct cgctcttggc gatctcgaag atgaccgtgc
    18781 ccgcgtcccg ctccagcgcg gcggcgaacg cctggcgcgc ggtgaggaag gtggtggcgc
    18841 ggacgttgat ggccggaacg gtgaaggcgg tcggctcgat ctcgccgcgc ccgcgggcca
    18901 tgtacagctc gtggatggag gccgacccgc agcccagggc ctgcgaggcg gaccagatca
    18961 cccagcgcgc ggcctcacgc aggtcgccgt cgtcggcgag ggcggcggcg aaggccagct
    19021 catccatgag gtcgccgcgg aggcgggatt cgtcgtgcac ctcgaggccg gtttcggtga
    19081 tcgtggcgac cccggcgagg cgctcccgga ggtcggcgac ggtggcgatg gtcatgatcc
    19141 gtccatccta ctagcggagc ccggaggcga gggtgaaggc cagcagcggc gatgccagca
    19201 ggccgagagc ggcggccagc aggtaccagt cgcgcgccgc gcgcccatcg gcgacgccgc
    19261 gacgctgcag gccgcgcgtc caccaccgga ccgccgtgag cgcggcgacg tagaggaggg
    19321 cggccagggc caccacgccg accgcgaagg gtcgcaccgc ctcgcagtca ccgaaggcga
    19381 gcgggcctcc gggcgggcag ggcgaggtca gctccgcgcc gatgacggca ccggccgcga
    19441 gcaggacgaa cgcggaccag gcgaggtgga cgaggagcga gcggctcagc gtcgggcgag
    19501 cgccgcgctc tgccgggccg cctccgcggc ctcgcgtccg gtgatgcgcc ggaactcgaa
    19561 ccagaggtac acgcaggcgg ccgcccccag gccggcggcg atcgtcccga acccgatggc
    19621 ctgcagcggg aagcgcggcg gccagaagga accaccggcc aggttgaagg ccatgatcgg
    19681 gatgatcatc agcacggcca gcaggtagtt cagcaggacg aagatgcgga tcgccgacca
    19741 gccgaacccg cggcggccac gcgccgcctg caccagcagg gccagcacca ctaccagcgc
    19801 caggttggtc atgccgacgc cgttcgccac caggaagccg acgtcgtcgc tgaacttcag
    19861 gaacggcagc tccacggtga tcaggtagta cacgtagttc gggaggtaga agatgatgaa
    19921 gtacatgaac aggaagaggg gcacgccctt cagcacggcg acgagccagg tctcgtagcc
    19981 gtagagcatc cagttcatga agtcgtcggc gccaccgagc agccggctca tcggcccgcg
    20041 tgggcggtag gcgggatacg agctcatcgc ttactcaccc gtgtcgtcca ggaggaagct
    20101 gatcagggcc tgcagcgagc tcgcgttgag cgagctctcc gggtgcggcg gcatcccggt
    20161 cggctggccg tcgttgtagt tgcccgccga gtcgcggatc cacttctcga ggaacatgcg
    20221 cgggtcgtcc tggtagtcgg cgatcatctg gtcgacgccc gacggcgtga cgggcaggcc
    20281 ctcggtgatc acgtcgtcct tgatgccggt gatatccggc cccgcggccg tcccggccag
    20341 gtcgggaccg tggcacgacg cgcagccggc gctcgcgtac acctcggcgc ccgcctcgat
    20401 cgtgttcggg acgtcctccg gcggctggtt ggccgcctcc gccgcccagg actcgac
//
LOCUS       DASTYP010000041         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_694951, whole genome shotgun sequence.
ACCESSION   DASTYP010000041 DASTYP010000000
VERSION     DASTYP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694951"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFV59_02220"
     CDS             complement(<1..713)
                     /locus_tag="VFV59_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU5324674.1"
                     /translation="MKLHLVDATFELFRAFYSRPPRRAPDGRPVNAVQGLIDSMLSLL
                     REPDVTHIGAATDHVIESWRNDLYPGYKSSLGVDPELLAQFIDAERALSALGIALWPM
                     VEDEADDAIAAAVHRFADDPRLEQVVICSVDKDLGQLVRDERIVLRDRIRKVTYDEGG
                     VVEKFGVAPTSIPDYLALVGDSSDGFPGLPGWGAKSAAAVLAKFGHLEAIPDSPLDWE
                     LDIRNASRLAATLAEHRPEA"
     gene            758..1126
                     /locus_tag="VFV59_02225"
     CDS             758..1126
                     /locus_tag="VFV59_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5324675.1"
                     /translation="MTHTAIDDFLSALPDDQRRALEALQARIRAIVPEATERVSYGVP
                     AFILDERPLVSVGAARTHCSFYVQSTAVIDAFADELRAAGFRVTAGSVLFQPDRPIPE
                     ALVERLVRARVEAVRARKRR"
     gene            1285..2094
                     /locus_tag="VFV59_02230"
     CDS             1285..2094
                     /locus_tag="VFV59_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5324676.1"
                     /translation="MRDDVIGRVIRALRHRRGWRQVDLSRAAGVSRAVLSDLEAGRLD
                     RHSLGALRAAITAAGGHLRFAVDVPGGELERLLDADHAHLQNRWKRWLEQHGWLVEAE
                     VTFNHFGERGSVDLLAFHPGRRVLLVVEIKSVLVDVQLALSTLDRKVRISEVLARERT
                     WQPIARIPVLAIREGSTARRRVAHHASLFGRFDVRGRTAVGWLMRPRAPTPTGILMLT
                     KSSDARPGDARRAGRQRVRAARRHSRSAGTSSGRRMSPGQTADDSAPSEPA"
     gene            complement(2232..>3245)
                     /locus_tag="VFV59_02235"
     CDS             complement(2232..>3245)
                     /locus_tag="VFV59_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5324677.1"
                     /translation="VEWGTRELPRWNTISISGYHIREAGSTAVQELAFTIADGIAYVE
                     DALSRGMRFDDFAPRLSFFFNSHNDFFEEIAKFRAARRIWYKLCRERFGAENERSTWM
                     RFHTQTAGVSLTAQQPLNNLTRVAVQALAGVLGGTQSLHTDAYDEAWAVPSEEAALVA
                     LRQQQILAEEAGVANTVDPLAGSYFVETLTNQTEQAAWRYIAQIDEMGGMVRALEAGY
                     PQAEIADAAYEQQREVEAHEREIVGVTRYADPDEELRIPLLEISREQERRHLDRLARV
                     RATRDAEAHASAMRRLEDEAHRGDVNLMPAIMDAVAAYATIGEMCGILRRVYGEYKEP
                     LAV"
BASE COUNT          465 a         1130 c         1151 g          499 t
ORIGIN      
        1 gcctcgggcc gatgctccgc gagcgtggcc gccagccggc tggcgttccg gatgtccagc
       61 tcccaatcga gcggcgagtc ggggatggcc tcgaggtgcc cgaacttcgc gaggacggcg
      121 gcggcagact tggctcccca cccgggcagt ccggggaagc cgtcgctgct gtcgccgacg
      181 agggcgaggt agtccgggat gctggtcggc gcgacgccga acttctcgac gacgcctccc
      241 tcgtcgtacg tcaccttccg gatgcggtcg cgcaggacga tgcgctcgtc gcgcaccagc
      301 tgacccaggt ccttgtcgac tgagcagatc accacctgct ccaggcgagg gtcgtccgca
      361 aaccgatgca ccgccgccgc gatggcgtcg tcggcctcgt cctcgaccat cggccacagc
      421 gcgataccga gggcggacag cgcccgctcc gcgtcgatga actgggccaa gagctccggg
      481 tcgacgccga ggctcgactt gtacccggga tacaggtcgt tgcgccacga ctcgatgacg
      541 tggtcggtgg ccgcgccgat gtgcgtcacg tccggctcgc gcaggagcga cagcatcgag
      601 tcgatcagcc cctggacggc gttcaccggc cgaccatcgg gcgcccgacg cggcggccgc
      661 gagtagaagg cgcggaagag ctcgaaggtg gcgtcgacga ggtggagctt catgggtcct
      721 ggcgaggata gcgggcgggt agcatgccgg cagaccgatg acccataccg ccatcgacga
      781 cttcctctcc gccctgccgg acgaccagcg gagggcgctg gaggcgttgc aggccaggat
      841 ccgcgcgatc gtcccggagg cgaccgagcg agtcagctac ggcgtgccgg ccttcatcct
      901 cgatgagcgg cccctcgtca gcgtcggcgc tgcccggacc cactgctcct tctacgtcca
      961 gagcacggcg gtgatcgacg cgttcgccga cgagctgcgg gcggcaggct tccgcgtgac
     1021 cgcgggctcg gtgctcttcc agccggatcg acccattccc gaggccctcg tcgagcgcct
     1081 cgtccgggcg cgcgtggagg cggtccgagc gcgcaagcgt cgctgatccg catcggtaag
     1141 ggcgcagtaa gcggctggta agcggccgtg tggatgatgg cggtcgcggc gtggagaagc
     1201 gtgccctgcg ccgccggccc atggttcccg gccggggtcg gcggcccacg ccgctcctct
     1261 gcggagcagt cgaggtgcct gaggatgcgg gacgacgtga ttggtcgcgt catccgcgcc
     1321 ctgcgacacc gcagaggttg gcgccaggtt gacctctcgc gcgctgccgg cgtgtcgcgc
     1381 gccgtgctct cggatctcga ggctggccgg ctggaccggc actcgttagg ggcactccgt
     1441 gccgccatca ccgctgccgg aggccacctt cggttcgcgg tggacgtgcc tggaggcgaa
     1501 ctcgagcggc tgctcgacgc cgatcacgca cacctccaga accgttggaa gcgctggctc
     1561 gagcagcatg gctggctggt cgaggcggag gttactttca accacttcgg cgagcgcgga
     1621 agcgtcgacc tgctggcgtt ccatcccggg cgtcgcgtcc tgctggtggt cgagatcaag
     1681 agcgtccttg tcgacgtgca actcgcgctg tccacgctcg atcgcaaggt gcggatctcc
     1741 gaggtgctag cccgagagcg gacctggcag ccgatcgccc gcatcccggt cctggcgatc
     1801 cgcgagggat cgacggcccg tcggcgagtc gcccaccacg ccagcctctt cggccggttc
     1861 gacgttcgcg gccggacggc ggtcggctgg ctgatgcgtc cccgggctcc gacgccgacc
     1921 ggcatcctga tgctcacgaa gtcgtcagat gcgcgtccag gcgacgctag acgggctgga
     1981 cgccagcgag tgcgtgcggc gcgtcgacac tcacgctcag ccggcacttc gagcggtcgc
     2041 cgaatgtcgc caggacagac ggctgacgac tccgcgccca gcgagcccgc ctaacgtcgc
     2101 caggacggac ggctgacgac tccgcgctca gcgagcccgc ctaacgtcgc caggacggac
     2161 gattgacgac tcggcgcgga cccgcccgtc taccgtcgct cggcgcggcc gagttgccga
     2221 gcgccgatgc gctagaccgc cagcggctct ttgtactcgc cgtagacgcg acgcaggatg
     2281 ccgcacatct cgccaatcgt ggcgtaggcg gcgacggcgt ccatgatggc gggcatgagg
     2341 ttgacgtcgc cgcggtgcgc ctcgtcctca agccgccgca tggcggaggc gtgggcctcg
     2401 gcgtcgcggg tcgcacgcac ccgcgccagc cgatcgaggt gccgtcgctc ctgctcccgc
     2461 gagatctcca gcagggggat ccggagctcc tcgtcgggat cggcgtacct ggtgacgccg
     2521 acgatctcgc gctcgtgcgc ctcgacctcg cgctgctgct cataggccgc atcggcgatc
     2581 tcggcctgcg ggtagccggc ctccagggcc cggaccatgc cgcccatctc gtcgatctgc
     2641 gcgatgtagc gccacgctgc ctgctcggtc tggttcgtca gcgtctcgac gaagtaggag
     2701 ccggccagcg gatcgaccgt gttcgccaca ccggcctcct cggccaggat ctgctgctgc
     2761 cgcagggcga cgagggcggc ctcttccgag ggaaccgccc acgcctcgtc gtacgcatcg
     2821 gtatggagcg actgcgtgcc ccccaggacg ccggccagcg cctgcaccgc cacccgggtc
     2881 aggttgttga gcggctgctg cgcggtcagc gagacaccgg ccgtctgggt gtggaaccgc
     2941 atccaggtgg agcgctcgtt ctcggcgccg aagcgctcgc ggcagagctt gtaccagatg
     3001 cgccgcgccg cccggaactt ggcgatctcc tcgaagaagt cgttgtggct gttgaagaag
     3061 aacgagaggc gcggcgcgaa gtcgtcgaaa cgcatcccac gggacagggc gtcctcgacg
     3121 taggcgatcc cgtcggcgat ggtgaaggcg agctcctgga ccgcggtgga gccggcctcg
     3181 cggatgtggt agcccgagat cgagatggtg ttccagcgcg gcagctcgcg cgtgccccac
     3241 tcgac
//
LOCUS       DASTYP010000042         4864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_695072, whole genome shotgun sequence.
ACCESSION   DASTYP010000042 DASTYP010000000
VERSION     DASTYP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695072"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFV59_02240"
     CDS             <1..895
                     /locus_tag="VFV59_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324678.1"
                     /translation="SLSVMRLIERPGKIVEGEIMLNGENLLQRTDEEMQDIRGDQVSM
                     IFQEPMTSLNPVFTCGDQISEAVQQHTGASAREGWDRAVEMLGLVGIPDPQRRAKEYP
                     HQLSGGMRQRVMIAMALSTNPDLLIADEPTTALDVTIQAQILELMRSLRQKNRMAIML
                     ITHDLGVVAEMADQVVVMYAGKVVERGDVRTIFGTPHHPYTQGLLHSIPRLDERVGRL
                     EVIKGTVPSPYNLPTGCLFKRRCPRRMPVCDVAPPYRVVTDGHLSRCWLTPAGEEPEV
                     GADAPAEEAEVAAEQLTVEPR"
     gene            892..1983
                     /locus_tag="VFV59_02245"
     CDS             892..1983
                     /locus_tag="VFV59_02245"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU5324679.1"
                     /translation="MTSQTIDTSGNVTSSGQTSQQRSAARGGDSSAPLLQVNDLVKHF
                     DIRGGFFGVSKLGAVRAVDGVSFTVNEGETLGLVGESGCGKTTLGKVILRLLPPTAGT
                     VNFEGRQLFDLTPRELKAARRDIQVVFQDPYASLNPRMSVGEIVGEGLFVHGMTNRRE
                     RESAVRDLLKRVGLNQQHIHRYPHEFSGGQRQRIGIARALALRPKFIVCDEPVSALDV
                     SIQSQVLNLLSDLQDEFQLTYLFIAHNLAVVRHLSTRVGVMYLGKLVELADADDLYEN
                     PKHPYTKALLSAIPIPDPTVRRERIVLKGDLPSPANPPSGCRFHTRCPMAQEICKVEE
                     PPLRDVGGGHMSACHFAEEVTPLRERHVA"
     gene            complement(2012..3292)
                     /locus_tag="VFV59_02250"
     CDS             complement(2012..3292)
                     /locus_tag="VFV59_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5324680.1"
                     /translation="MTGPASGIGPRERRWLVIFLALGSAYFAVLLAERFLGIVGGIGT
                     IVLILFLAWLLAFVMSPVVSAIERAVDLPRPAIVVGAYVLALVIVGFVLFYTGSAITQ
                     QVAELARNYPETERRILRTLLEWEQSLHFGRLEIDLTDLYGGVAREFERLGADIVEQA
                     QAIAGVTIAALGSLLLIIVLSLYMLMDSGRILARLRTAVPRRYTDEAVLFERSIVRAF
                     GGFLRAQLILAVLQAVLVAIVGTVFGVPYLFLWGTISALAMLIPFFGPALALVPPIIG
                     AILFAPDAALPVALILLVVQTVMVNWLQPRLMRGALGLHPILVLVGLLLGAQVAGVWG
                     ALFGIPIIAVVWVFVSYLVFGTVPNAALPEAERLADVDEHVMVSVAKEQVGDETHPHI
                     HVTRMRRADGSEQVELRMAEPPTPQPPPRPSEEG"
     gene            complement(3295..4320)
                     /gene="trpS"
                     /locus_tag="VFV59_02255"
     CDS             complement(3295..4320)
                     /gene="trpS"
                     /locus_tag="VFV59_02255"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5324681.1"
                     /translation="MSQTLDAPAGESAATRERVFSGIQPSGASHLGNYLGAQVNYVAL
                     QDRYDAIYAIVDLHALTTVHDGRQLRDLSHEMVLDLLAIGLDPQRSALIRQSDFPEHA
                     ELTWIFNTVTPVSWAERTPTYKEKKQQGIENSMGLLDYPILQAADIVIYKASRVPVGR
                     DQAAHLELSREVVRAFNRRYGAIFPEPEPVFTDAPTVLGIDGVAKMSKSAGNTIPIFA
                     EPDEIHRLVMSMVTDPERIKRTDPGRPEICNVCQLHRFFGGDYLQIQDGERTARTGCV
                     DTKKLLAERIIEFFRPMRERRQQLAADPAVVEETLAAGAAKVRPIIEATMAEVRAAVG
                     IGPRRAV"
     gene            4358..>4864
                     /locus_tag="VFV59_02260"
     CDS             4358..>4864
                     /locus_tag="VFV59_02260"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU5324682.1"
                     /translation="MNEPPTVRWSDAARPGGRIGHLVEAHATIGSTSDRARALLAAGG
                     DGIAVVADEQTTGRGRHGRHWLTPPGRGLALSVGVRTTVAARDGWQLAFAAGLAARRA
                     CDADAEIGLKWPNDVVSPDGRKIGGILVETTVERERIATAVFGIGLNVDWPADEMPDE
                     IAGTATSLV"
BASE COUNT          766 a         1688 c         1740 g          670 t
ORIGIN      
        1 cagcctgagc gtcatgcggc tgatcgagcg cccgggcaag atcgtcgagg gcgagatcat
       61 gctcaacggc gagaacctgc tccagcgcac cgacgaggag atgcaggaca tccgcggcga
      121 ccaggtcagc atgatcttcc aagagccgat gacttcgctg aaccccgtgt tcacgtgcgg
      181 cgaccagatc agcgaggcgg tgcagcagca caccggcgcc tccgcccgcg agggctggga
      241 ccgcgccgtg gagatgctcg gcctggtggg catcccggat ccgcagcgac gcgcgaagga
      301 gtacccgcac cagctgtcgg gcggcatgcg ccagcgcgtg atgatcgcca tggcgctgtc
      361 gaccaacccg gacctgctga tcgcggacga gccgaccacc gccctggacg tgaccatcca
      421 ggcccagatc ctggagctga tgcgctcgct gcgccagaag aaccggatgg cgatcatgct
      481 catcacgcac gacctgggcg tggtggccga gatggccgac caggtggtgg tcatgtacgc
      541 cggcaaggtg gtggagcgcg gcgacgtgcg caccatcttc ggcaccccgc accatcccta
      601 cacgcagggc ctcctgcact cgatcccgcg cctcgacgag cgcgtcggcc gcctcgaggt
      661 gatcaagggg acggtgccct cgccctacaa cctgccgacc gggtgcctct tcaagcgtcg
      721 ctgtccgcgc cgcatgccgg tgtgcgacgt ggccccgccg taccgcgtgg tcaccgacgg
      781 gcacctgtcc cgctgctggc tgaccccggc gggcgaggag ccggaggtcg gcgccgatgc
      841 gccggccgag gaggccgagg tcgcggccga gcagctgacg gtggagccac gatgaccagc
      901 cagacgatcg acaccagcgg caacgtcacc agctcggggc agacctcgca gcaacgctcc
      961 gccgcgcgag gcggtgactc gagcgctccg ctcctgcagg tcaacgacct ggtcaagcat
     1021 ttcgacatcc gcggcggctt cttcggggtg agcaagctcg gcgccgtgcg cgccgtggac
     1081 ggggtctcgt tcaccgtcaa cgagggcgag accctcggcc tggtgggcga atccggctgc
     1141 ggcaagacca ccctcggcaa ggtgatcctc cgcctgctgc cgccgactgc gggcaccgtc
     1201 aatttcgagg ggcgccagct cttcgacctc acgccacgcg agctgaaggc cgcccggcgc
     1261 gacatccagg tggtcttcca ggatccgtat gccagcctca acccgcgcat gagcgtcggg
     1321 gagatcgtcg gcgaggggct gttcgtccac ggcatgacca accgccgcga gcgggagtcg
     1381 gcggtgcgcg acctgctcaa gcgcgtcggc ctgaaccagc agcacatcca tcggtacccg
     1441 cacgagttct cgggtgggca gcggcagcgc atcggcatcg cccgcgcgct cgccctgcgg
     1501 ccgaagttca tcgtctgcga cgagccggtg tcggcgctgg acgtctcgat ccagtcgcag
     1561 gtgctcaacc tgctcagcga cctccaggac gagttccagc tcacctacct gttcatcgcc
     1621 cacaacctgg ccgtggtgcg gcacctgtcg acccgggtcg gcgtgatgta cctgggcaag
     1681 ctggtggagc tggccgatgc cgacgacctg tacgagaacc cgaagcaccc gtacaccaag
     1741 gccctgctgt cggccattcc gatcccggac ccgacggtgc ggcgcgagcg gatcgtgctg
     1801 aagggtgacc tgccgtcgcc ggccaacccg ccgtccggct gccgcttcca cacccgctgc
     1861 ccgatggcgc aggagatctg caaggtcgag gagccgccgc tgcgggacgt cggtggcggc
     1921 cacatgagcg cctgccactt cgccgaggag gtcacgccgc tccgcgagcg gcacgtcgcc
     1981 tgatcgctga tcaggcccgg cgcccgggcg cctagccctc ctcggacggc cgcgggggcg
     2041 gctggggcgt cggaggctcg gccatgcgca gctcgacctg ctcgctgcca tccgcgcgcc
     2101 gcatccgcgt gacgtggatg tgcgggtggg tctcgtcgcc cacctgctcc ttggccacgc
     2161 tcaccatcac gtgctcgtcg acgtcggcga ggcgctccgc ctcgggcagc gccgcgttcg
     2221 gcaccgtccc gaacaccagg tagctgacga agacccagac gacggcgatg atcgggatgc
     2281 cgaacagcgc gccccacacg ccggcgacct gggcgccgag cagcaggccg accaggacga
     2341 ggatcgggtg caggccgagg gcgccgcgca tgagtcgcgg ctgcagccag ttgaccatca
     2401 cggtctgcac gaccaggagg atcagggcga ccggcagggc cgcgtccggc gcgaagagga
     2461 tcgcgccgat gatcggcggc acgagcgcga gcgccggtcc gaagaacggg atcagcatcg
     2521 ccagcgcgct gatggtgccc cacaggaaga ggtagggcac gccgaacacc gtgccgacga
     2581 tggcgaccag gaccgcctgc agcacggcca ggatgagctg ggcgcggagg aagccgccga
     2641 aggcccgcac gatgctgcgc tcgaacagca ccgcctcgtc cgtgtagcgg cgcgggaccg
     2701 cggtccgcag acgggcgagg atgcggcccg agtccatcag catgtagagc gacaggacga
     2761 tgatcaggag cagggacccg agggccgcga tggtgacgcc ggcgatggcc tgcgcctgct
     2821 ccacgatgtc cgcgcccagg cgctcgaact cgcgcgcgac gccaccgtag aggtcggtca
     2881 gatcgatctc cagccgaccg aagtgcagcg actgctccca ctccagcagc gtgcgcagga
     2941 tgcgacgttc cgtttcgggg tagttgcggg cgagctcggc gacctgctgg gtgatggccg
     3001 acccggtgta gaacaggacg aacccgacga tgacgagcgc cagcacgtag gcgccgacca
     3061 cgatcgccgg gcgcggcagg tcgacggccc gctcgatggc gctcaccacc ggcgacatca
     3121 cgaaggcgag gagccacgcc aggaagagga tcagcacgat cgtgccgatg ccgccgacga
     3181 tgccgaggaa gcgctccgcc agcagcacgg cgaagtacgc gctgccgagc gccaggaaga
     3241 tgacgagcca gcggcgttcg cggggaccga tgcccgatgc cggcccggtc acgcctacac
     3301 cgcccggcga ggaccgatgc cgaccgcggc gcgcacctcc gccatggtgg cctcgatgat
     3361 cggtcgcacc ttcgccgcac ccgcggcgag cgtctcctcg acgaccgccg ggtcggccgc
     3421 gagctgctgc cggcgctcac gcatcggccg gaagaactcg atgatccgct cggcgaggag
     3481 cttcttggtg tcgacgcagc cggtgcgcgc cgtccgctcg ccatcctgga tctgcaggta
     3541 gtcccccccg aagaaccgat gcagctggca gacgttgcag atctccggtc gcccggggtc
     3601 ggtgcgcttg atgcgctccg ggtcggtcac catgctcatc accagccggt ggatctcgtc
     3661 gggctcggcg aagatgggga tggtgttgcc ggcgctcttg ctcatcttgg ccacgccgtc
     3721 gatgccgagc acggtcggcg cgtcggtgaa gaccggctcc ggctccggga agatggcgcc
     3781 gtaacgccgg ttgaaggcac gcaccacctc tcgcgacagc tcgaggtgcg ccgcctggtc
     3841 ccgaccgacc gggacgcggc tggccttgta gatgacgatg tccgccgcct gcaggatggg
     3901 atagtcgagc aggcccatcg agttctcgat gccctgctgc ttcttctcct tgtaggtcgg
     3961 cgtgcgttcc gcccagctca ccggcgtgac cgtgttgaag atccaggtca gctcggcgtg
     4021 ctcggggaag tcggactggc ggatcagggc ggagcgctgc gggtcgagcc cgatggccag
     4081 caggtcgagc accatctcgt ggctcaggtc gcgcagctgt cggccgtcgt ggacggtggt
     4141 cagcgcgtgc aggtcgacga tcgcgtagat ggcgtcgtat cggtcctgca gcgcgacgta
     4201 gttgacctgc gcgccgaggt agttcccgag gtggctggcc cccgagggct ggatgccgct
     4261 gaagacgcgc tcccgcgtcg cggccgactc gccggcaggg gcgtcgaggg tctggctcat
     4321 gggacgccga gtgtagcgca cggtatcgtg tgcgccgatg aacgagccgc cgaccgtccg
     4381 ctggtccgat gccgcgcgcc ccggcgggcg aatcgggcac ctggtggagg ctcacgcgac
     4441 gatcgggtcg acgagcgatc gcgcccgagc tctgctggcg gcggggggcg acgggatcgc
     4501 cgtggtggcc gacgagcaga cgacggggcg tggccgccac ggtcgccact ggctgacgcc
     4561 acccggccgc gggctggcgc tgagcgtcgg cgtgcggacc accgtggccg cgcgtgacgg
     4621 ctggcagctg gcgttcgcgg ccgggctcgc ggcccgccga gcgtgcgacg ccgatgccga
     4681 gatcggcctg aagtggccga acgacgtggt gtcgccggac ggccgcaaga tcggcggcat
     4741 cctggtcgag accacggtcg agcgcgaacg gatcgccacc gccgtcttcg ggatcggcct
     4801 gaacgtcgac tggcccgcgg acgagatgcc cgacgagatc gccggcaccg ccacgtcgct
     4861 ggtc
//
LOCUS       DASTYP010000043         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1751460, whole genome shotgun sequence.
ACCESSION   DASTYP010000043 DASTYP010000000
VERSION     DASTYP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751460"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFV59_02265"
     CDS             <1..801
                     /locus_tag="VFV59_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005446051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5324683.1"
                     /translation="VEFRTGRPVSPPIMPGWDGYPVRERFTDRYAAPVWVDNDVNIMA
                     LGEWRAGIAVGHANVIVMKIGTGIGAGIISNGRIHRGANGAAGDVGHIQVVDDASITC
                     RCGNVGCLEALAGGAALARDARTAALAARSEQLRSSLDRHGDVTVEDLARAASRGDAV
                     AVDMLGAAGHRIGLMLASIVNFFNPSLVVIGGGVAQAGEPLLGAIRETVYRRSLPLAT
                     RELAIEHSSLGPLAGLIGASTMALDELFSRDAMAGRLAQRADEVATTR"
     gene            complement(830..1555)
                     /locus_tag="VFV59_02270"
     CDS             complement(830..1555)
                     /locus_tag="VFV59_02270"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU5324684.1"
                     /translation="MTGFHLDVFPAERWADAVAADLADRLREQPALRLCLPTGSTPAP
                     VYASLVRSTDPSLWSAATIMLLDEYLGLPPGDPARGGDRLVRELVDHVHPARFVAIDA
                     DAADPEPASRAHDAEAARGLDLALLGLGLNGHVGLNEPGSDADAETRVVELTPTSREV
                     AAGYGATGAPQRGVTLGLRRLLESREIWLLVTGERKAEILGRTLLGPEGSDVPATFLR
                     RHPNLRVIADEPAARLVAGGVLG"
     gene            complement(1552..2751)
                     /locus_tag="VFV59_02275"
     CDS             complement(1552..2751)
                     /locus_tag="VFV59_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5324685.1"
                     /translation="MRSHQITMLGTGLIGDFYTATLHGQRGRDRVRVVYSRSEERGRA
                     FSERWGIEDSTTSIEEAVRHPETDVVVVGLPNFLHEEAVAAAAAAGKAVLITKPLGRN
                     AEEARRILETVEKAGVFGGYLEDLCYTPKTLKAVRSVEGGALGDVTWVRSREAHPGPH
                     SAWFWDGRLTGGGAIVDLGCHCIEIIRNFVGKQNRPVEVMCHTDTLVHPIEDEDNAIA
                     LIRFESGAIGQFEVSWTFRGGMDLRDEVAGTHGTIWLNHFLRTGYEMFTAGGSSGYVA
                     EKAETTAGWLFPVGDEVSELGYVDMFSDMFDALDNGRQPRETLYDGYVVNAVMDACYR
                     SAKSRQWEPVALFEWRGEGASRIAIEPQMHDGKVVIKRETLPDGREKLILKDPNSGDF
                     ADVVVAR"
BASE COUNT          435 a         1083 c         1075 g          460 t
ORIGIN      
        1 gtggagttcc ggacggggcg tcccgtctcg ccgccgatca tgcccggctg ggacggctac
       61 ccggttcggg agcggttcac ggaccgctac gccgcgcccg tctgggtcga taacgacgtg
      121 aacatcatgg cgctcggcga gtggcgggcg ggcatcgccg tggggcacgc caacgtgatc
      181 gtgatgaaga tcggcaccgg catcggcgcc gggatcatct ccaacggccg catccaccgg
      241 ggagcgaacg gcgccgcggg tgacgtcggc cacatccagg tcgtcgacga cgcgtccatc
      301 acctgccgct gcggcaacgt cggctgtctc gaagccctcg ccggtggcgc ggcgttggct
      361 cgtgacgcga ggacggcggc gctggcggcc cgcagcgagc agctgcggtc cagcttggac
      421 cgccacggcg acgtgacggt cgaggatctg gctcgcgccg catcgcgcgg cgacgcggtc
      481 gccgtcgata tgctgggcgc ggccggccac cgcatcggcc tgatgctggc gagcatcgtg
      541 aacttcttca acccttcgct ggtcgtgatc ggcggagggg tggcccaggc cggcgagccg
      601 ctgctcggcg ccatccgcga gacggtgtac cggcgctcgc tcccgctcgc cacccgtgag
      661 ctcgccatcg agcactcgtc actcgggccc ctggcagggc tgatcggcgc gtcgacgatg
      721 gcgctggacg agctgttctc gcgcgacgcc atggcgggcc gcctcgcaca gcgcgctgac
      781 gaggtcgcga cgacgcgata gagggcgcgc gtcgtcgtcg gacggcggtc tacccgagca
      841 cgccgcccgc caccagcctg gccgccggct cgtcggcgat gacgcgcagg ttcgggtgcc
      901 ggcgcaggaa cgtggccggg acgtccgacc cctcgggccc gagcagcgtg cgacccaaga
      961 tctccgcctt gcgttcaccg gtgaccagca gccagatctc ccgcgactcc aggaggcgcc
     1021 tcagtccgag ggtcacgcct cgctgcggcg cgccggtcgc gccgtagccc gcggcaacct
     1081 cccggctggt gggcgtcagc tccaccacac gcgtctccgc gtccgcgtcc gagccgggct
     1141 cgttgagccc gacgtgcccg ttcaagccca ggcccagcag cgccagatcc aggccgcgag
     1201 ccgcctcggc gtcatgcgcg cgcgacgccg gctccgggtc ggccgcatcg gcgtcgatgg
     1261 ccacgaagcg cgccgggtgg acgtggtcga ccagctcccg gaccaggcgg tcgcctccgc
     1321 gagcgggatc ccccggcgga aggccgaggt actcgtccag cagcatgatg gtcgccgcag
     1381 accacagcga cgggtcggta gaccgcacga gcgacgcata taccggcgcg ggcgtggacc
     1441 ccgtcggcag gcagagccgc agggcgggtt gctcgcggag gcgatcggcc aggtcagcgg
     1501 cgaccgcatc ggcccagcgt tcggccggga agacgtccag gtggaagccc gtcaacgcgc
     1561 gaccacgacg tcggcgaagt caccgctgtt cggatccttg aggatcagct tctcacgacc
     1621 gtctggcagc gtctcgcgct tgatgacgac cttgccgtcg tgcatctgcg gctcgatcgc
     1681 gatccgggat gcgccttctc cgcgccactc gaacagcgcg acgggctccc attggcggct
     1741 cttcgcggat cggtagcagg cgtccatgac ggcgttgacc acgtagccgt cgtacagcgt
     1801 ctcgcgcggc tgtcgcccgt tgtccagggc gtcgaacatg tcgctgaaca tgtcgacgta
     1861 gccgagctcg ctcacctcgt caccgaccgg gaacagccag ccagccgtcg tctcggcctt
     1921 ctcggcgacg tagccgctcg agccgcccgc ggtgaacatc tcgtagcccg tgcgcaggaa
     1981 gtggttcagc cagatggtgc cgtgcgtccc agccacctcg tcgcggagat ccatgccgcc
     2041 gcggaaggtc cagctgacct cgaactggcc gatggcgccc gactcgaagc ggatcagcgc
     2101 gatcgcgttg tcctcgtcct cgatcgggtg gaccagcgtg tcggtgtggc acatcacctc
     2161 gacggggcgg ttctgcttcc cgacgaagtt gcggatgatc tcgatgcagt ggcagccgag
     2221 gtccacgatg gcgccgccgc cggtcagccg accgtcccag aaccacgccg agtgcggacc
     2281 ggggtgcgct tcccgtgatc ggacccacgt cacgtcgccg agcgctccgc cctcgaccga
     2341 gcgcacggcc ttgagcgtct tcggggtgta gcacaggtcc tcgaggtagc cgccgaagac
     2401 gcccgccttc tcgacggtct cgaggatgcg gcgcgcctcc tcggcgttac ggccgagcgg
     2461 cttggtgatg aggacggcct tgcccgccgc agcggccgcg gcgacggcct cctcgtggag
     2521 gaagttcggg aggccgacga ccacgacatc ggtctcggga tggcgcaccg cctcctcgat
     2581 actggtcgtg gagtcttcga tcccccagcg ctcgctgaag gcgcggccgc gctcctcgct
     2641 gcgcgagtag acgacgcgga cgcggtcacg cccgcgctgg ccgtgcagcg tcgcggtgta
     2701 gaagtcgccg atgagcccgg tcccgagcat ggtgatctgg tgagagcgca cgcgcgtgct
     2761 ccttcgttgc ggttcagcgg ggcctcgatt gtaggttcgg cgacgctgcg cccgcatgac
     2821 gccgggggcc cgatcccggt ccgcgtcggg agaatcgcga cgctcggcat cttccttgcg
     2881 gctcctcttc gctgacgcaa caaggaggaa cgaatccgtg ttcgctcaga acgacaactg
     2941 tccgaagtgc gacatgcccg tcagccaggg cgacaacccg accgagagcg gcggcgagat
     3001 gttctgctgc cagggctgtg ccgagggtgg cgcctgcacc tgctctcagc acc
//
LOCUS       DASTYP010000044         8274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_440217, whole genome shotgun sequence.
ACCESSION   DASTYP010000044 DASTYP010000000
VERSION     DASTYP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440217"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /gene="tyrS"
                     /locus_tag="VFV59_02280"
     CDS             complement(<1..441)
                     /gene="tyrS"
                     /locus_tag="VFV59_02280"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5324686.1"
                     /translation="MTTPDARAAVLDDLEWRRMMADHTDLDDLRSAMRAAPLTFYGGF
                     DPTAPSLHFGNLVLLVTMRRLQLAGHRPIGLVGGATGLVGDPSGRTSERTLNEREVVA
                     SWVERIRSQVARYLDFDGDNGALIVNNLDWTGAMSAIDWLRDIGR"
     gene            594..3242
                     /locus_tag="VFV59_02285"
     CDS             594..3242
                     /locus_tag="VFV59_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU5324687.1"
                     /translation="MLAIFGVAAVGLFVVLMGFYASLTSGLPDVAEIENFDLAQGSTV
                     VSADGTELATFAIEDRREIDFDQIPQVMVDAQVAAEDQRFWTNPCVDFQSIVRAFLQN
                     VEAGGTVSGASTICQQLVRMRLFGAELMADPERQVERKLKEAILALRLDSRYPGEEGK
                     QRILEMYLNQVYYGNNAYGVWAAANAYFGKDLTSDAPEDQLTVSEAAMLAGLVRAPSR
                     LDPSREGVPEERGGETVYVVPQTSQAITVRNLVLDQMLRSGFIDQAQHDQAVNEEVIL
                     SPPREQTYKAPHFVFAVRREAAELLEGEDLLDTGGLRIITTLDYEGYQVIAEKWAQVA
                     YDMDRLSDDELVAKYGEDALSWIGQLQGRNINNDAIVTVNYRTGAVLAYVGSANFYGE
                     ATPAHQPNYDVIGQAFRQSGSAFKPITYAAGFESGVITPATMFMDVRTDIAEGFNPPN
                     ADGKERGPVRVRDALKYSLNVPVSKAQQLIGSQNVVDLAQRLGLEFDPAHNNEFAVPS
                     LTLGTLGIHQLDLAGAYGALANGGRLNEPYLIQRIEDADGNVIYDHDTDAEDPVQVVT
                     APSAYLVTDILADNTDPNTNPLWGPRFQLETADGGRRPATLKTGTTNDFRDLQAYGYL
                     AGNPDDPEDPTGAIVTGVWVGNSDFSPIQDVFAADGPTFIWHDYMAEIAAHNELPVYD
                     FARPDGVSEVTVDAITGLLPGEHTTTTITELFRTDRQPSQQDTAHRELRIEAETGKVW
                     QEGCGDFETAAPSGSPDPSAEPPPPQPKEEVYLDLSGWDADHPAWQEANQAWIEQWRG
                     RESELNQSVRVPFPGPLDAPLAPTEECTPGEIPTSTPTPSPTPTPSPTPTPSPTPEPT
                     PTPLLPTPTPILPIDTPTPIPTPVAP"
     gene            complement(3246..5684)
                     /locus_tag="VFV59_02290"
     CDS             complement(3246..5684)
                     /locus_tag="VFV59_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU5324688.1"
                     /translation="MTATSPADSLPAGAAADAATIRALEFAAIVEQLATNTSFQPSRE
                     LALEAVPVDDAVHVALLQDQTDEASRLLNEQAQASIGGARDVRGALERAARGGRLTAQ
                     ELLDLRETLDATGRFAARLSAWLGPHLAGIRDELDEAPELAQRIGRSVDESGEILDTA
                     SAELAAIRKRLRTAQDRVRERLNAMLRSSTMAGLIGEAIVTVRAGRYVIPIRAEAKGR
                     VKGIVHDQSASGATLFIEPLSVVELNNTWTEATLAEQREEDRILDELSRGVEARADAL
                     RASLLALARADHWMARARLGEQMDGVRPAVMADAVELLSARHPLLGPAAVPIDLRLGE
                     RFGYRALVVTGPNTGGKTVSLKTLGLLALMHQAGLRVPAAEGARLPVFRRVMADIGDE
                     QSIAQSLSTFSSHLRNVVRFVAAAGPDTLVLLDEVGAGTDPTEGSALAMAIVSQLLAA
                     GSMVAATTHYAELKTFATEHPGVTNAAVEFDVTTLRPTYRLSIGLPGKSQAFAIAERL
                     GLSPGILADARARISAEHVTMEETLAAIAQAREQQQEALEAATRERSQATAERERARA
                     GVSRARREAGEILTDARRAADDMLARAEREVAELRREMTRQRNLAGGRGSTNAAALEE
                     LGRRAARARSEAPPARPPARGADAEAETTDAAAPEVPQPRVGLWARSRTLGTTGRIVD
                     ISGRTGRATLETEGARLVVPADDLDVVPEPVSGPSPRDLQAEELRRRAAARVSPSIDL
                     HGERVEAALERLHAYLDEALLAGLDSVVIVHGVGTGALRRAVRDALREHPRVRSFRGG
                     RRDEGGEGATVAEL"
     gene            complement(5659..6273)
                     /locus_tag="VFV59_02295"
     CDS             complement(5659..6273)
                     /locus_tag="VFV59_02295"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU5324689.1"
                     /translation="MSAAAAAVPMTGIERHWFDRPSAELAPDLLGARLVHASPEGTVG
                     GRIVEVEAYRGPEDRAAHSSRGRTARNAVMFGPPGYLYVYLIYGLHHCLNVVAGPGEK
                     PEAVLIRALEIDEGLELAQARRGTTVPRHRLAAGPGNVGQALGVDRSLNGADLLAGPV
                     SIEPRVGAAPAIHRGPRIGVDYAGDWAARPLRFWIADDRHVSRR"
     gene            complement(6270..6821)
                     /locus_tag="VFV59_02300"
     CDS             complement(6270..6821)
                     /locus_tag="VFV59_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU5324690.1"
                     /translation="MDVLAQLGWMDLLIILVLAVGVFIGFTQGMIRYVLNCIAVLVAF
                     ILASQLTGPIVDLLRFWTAFTPEGRKLLIFVLLFLAFVVGGWFAIRALYRRTRLPIAK
                     QLDELGGAIFGLLWVALLITFHLLVFDTSFGPGADEGGWVPAYVKAMNDSLIVQFLRD
                     TLLPTAGFLVRPFVPDEVARLFP"
     gene            complement(6928..>8274)
                     /locus_tag="VFV59_02305"
     CDS             complement(6928..>8274)
                     /locus_tag="VFV59_02305"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324691.1"
                     /translation="IGPDLPRFAADRAAQLIGEITGARVARGIVDNDPGPHPRRVVGA
                     SVERMARLLGVPLAPAAVRQLLEPLGFDVSGDGDALEIAVPPQRLDVVAEADVAEEVA
                     RAYGYDRIPGRLPVAALPPYRPDPSGPRHRVRRILAGLGLDEVVTHALIGPADLSRTA
                     LPADDPELVRLYNPLSEEHSILRPSMAPSLLGALAENVRRRRPDAWLFDLGKVYWYHP
                     DAPTPRDRTSETAGTGRYESWELGIILSGAAAAPAAGEAALPADVAAIKGVVEALHDA
                     IGAPRPTYRPEPEGSRHAHRHPGRTALICDAAGRPYGSLGEVHPRVVEAWDLVGRPVD
                     AALDVGRLLELVQPEHRIRPVPGAQPVDRDLAVVLDDATPVGELLRVARMAAGPLLEE
                     LRLFDVYRGEQIGRGRVSYALAFRFQPGEAGEEKAIEKALNKVRGSLRHHLGAEIR"
BASE COUNT         1215 a         3071 c         2915 g         1073 t
ORIGIN      
        1 tcggccgatg tcccgcagcc agtcgatggc cgacatcgca cccgtccagt ccaggttgtt
       61 gacgatcagc gccccgttgt cgccgtcgaa gtccaggtag cgcgcgacct gcgagcggat
      121 gcgctcgacc caggaggcga ccacctcgcg ctcgttgagg gtgcgttcgc tggttcgccc
      181 gctggggtcg ccgaccagcc cggttgcgcc tccgacgagc ccgatcggcc gatgtcccgc
      241 gagctggagt cgacgcatgg tgaccagcag cacgaggttg ccgaagtgga ggctgggcgc
      301 ggtgggatcg aagccaccgt agaacgtcag cggcgccgcg cgcatcgccg agcgcaggtc
      361 gtcgagatcg gtgtggtcgg ccatcatccg ccgccactcg aggtcgtcga gcacggcggc
      421 gcgggcgtca ggggtcgtca tcggcggttg aggatagcac cgaacatccg tgctaccctg
      481 cccggcggat gacccgacga ccagctccga gtggccgtcg cgacgcgccc cgctccgccg
      541 gccgtggagc gccgacgcgc cgccggcccg gctccaccac cctcccccgc accgtgctgg
      601 cgatcttcgg cgtcgcggcc gtcgggctgt tcgtggtgct gatgggcttc tacgcgtcgc
      661 tgaccagcgg cctgcccgac gtggcggaga tcgagaactt cgacctggct caggggtcca
      721 ccgtggtctc ggccgacggc accgagcttg cgaccttcgc catcgaggac cggcgcgaga
      781 tcgacttcga ccagatcccg caggtcatgg tggatgccca ggtggccgcg gaggaccagc
      841 gcttctggac caatccgtgc gtcgatttcc agagcatcgt gcgggccttc ctgcagaacg
      901 ttgaggcggg aggcaccgtg tcgggcgcct cgaccatctg ccagcagctc gtccggatgc
      961 gcctcttcgg cgccgagctg atggccgatc cggagcgcca ggtcgagcgc aagctcaagg
     1021 aggccatcct ggcgctccgg ctcgacagcc gctaccccgg cgaggagggc aagcagcgca
     1081 tcctggagat gtacctgaac caggtctact acgggaacaa cgcctacggc gtgtgggccg
     1141 ccgccaacgc ctacttcggc aaggacctga cctccgatgc gcccgaggac cagctcaccg
     1201 tcagcgaggc ggccatgctg gccggcctgg tccgcgcgcc gtcccgtctc gacccgagcc
     1261 gtgagggcgt ccccgaggag cggggcggcg agaccgtgta cgtggtgcca cagacctcgc
     1321 aggcgatcac cgtgcgcaac ctcgtcctcg accagatgct gcgatccggg ttcatcgacc
     1381 aggcccagca cgaccaggcc gtgaacgagg aggtcatcct ctccccgccg cgcgagcaga
     1441 cctacaaggc tccgcacttc gtcttcgccg tgcggcgcga ggcggccgag ctgctggagg
     1501 gcgaggatct gctggacacc ggcgggttgc gcatcatcac caccctcgat tacgagggct
     1561 accaggtcat cgccgagaag tgggcgcagg tcgcctacga catggaccgg ctgtccgacg
     1621 acgagctggt cgccaagtac ggcgaggatg cgctgtcctg gatcgggcag ctccagggca
     1681 ggaacatcaa caacgacgcc atcgtgacgg tcaattaccg cacgggcgcg gtgctcgcct
     1741 acgtcggcag cgcgaacttc tacggcgagg cgaccccggc gcaccagccg aactacgacg
     1801 tcatcgggca ggccttccgc cagtccggct ccgccttcaa gccgatcacc tacgccgccg
     1861 gcttcgagtc gggcgtcatc acgccggcca cgatgttcat ggacgtccga accgacatcg
     1921 ccgagggctt caacccgccc aacgccgacg gcaaggagcg cggtccggtg cgcgtccgcg
     1981 acgcgctgaa gtactcgctg aacgtgccgg tcagcaaggc gcagcagctc atcggctcgc
     2041 agaacgtggt cgacctggca cagcgcctgg gcctcgagtt cgatccggcc cacaacaacg
     2101 agttcgccgt cccgtcgctg acgctgggca cgctcggcat ccaccagctc gacctggccg
     2161 gcgcgtacgg ggcgctggcc aacggcggac ggctgaacga gccgtacctg atccagcgca
     2221 tcgaggacgc cgacggcaac gtcatctacg accacgacac cgatgccgag gacccggtcc
     2281 aggtcgtgac cgccccatcg gcctacctgg tgaccgacat cctggccgac aacaccgatc
     2341 cgaacaccaa cccgctgtgg ggcccgcgct tccagctcga gacggcggat ggcggccgcc
     2401 gccccgccac gctgaagacc ggcaccacga acgacttccg cgatctccag gcgtacggct
     2461 acctcgccgg caaccccgac gatccagagg atccgaccgg cgcgatcgtg accggggtct
     2521 gggtcggcaa cagcgacttc tcccccatcc aggacgtgtt cgccgccgat ggcccgacct
     2581 tcatctggca cgactacatg gcggaaatcg ccgcccacaa cgagctgccg gtgtacgact
     2641 tcgcgcgccc cgacggcgtc tccgaggtga ccgtcgacgc catcaccggc ctcctgccgg
     2701 gcgagcacac caccaccacg atcaccgagc tgttccggac cgatcggcag ccgtcacagc
     2761 aggacacggc gcaccgcgag ctgcggatcg aggcggagac cgggaaggtc tggcaggagg
     2821 gctgcggcga cttcgagacc gccgcaccgt ccgggtcgcc ggacccgtcg gccgaaccac
     2881 cgccgcctca gccgaaggag gaggtctacc tcgacctgtc cggctgggac gccgatcacc
     2941 cggcgtggca ggaggcgaac caggcgtgga tcgagcagtg gcgcggccgg gagtcggagc
     3001 tgaaccagag cgtccgggtg cccttccccg gtccgctcga cgcgccgctg gccccgaccg
     3061 aggagtgcac gccgggcgag atcccgacct ccacgccgac accgtcaccg acgccgacgc
     3121 ccagccccac cccgaccccg tcgccgacac cggagccgac gccgacgccg ctgctgccga
     3181 cgccgacgcc gatcctgccc atcgacacgc cgacgccgat accgaccccc gtcgcgccct
     3241 agcggctaga gctcggcgac cgtcgccccc tcgccgccct cgtcgcgccg cccgccgcgg
     3301 aacgatcgga cgcgcggatg ctcgcgcagc gcgtcgcgca ccgcccgtcg aagggcgccc
     3361 gtgccgacgc cgtggacgat gaccacggag tccagcccgg ccagcagcgc ctcgtccagg
     3421 taggcgtgca gccgctcgag cgcggcctcg acccgctccc cgtgcaggtc gatgctgggt
     3481 gacacgcgcg ccgctgcgcg acggcgcagc tcctcggcct gaaggtcgcg cggtgagggg
     3541 ccgctcaccg gctcggggac gacgtcgagg tcgtcggccg gcacgaccag gcgcgccccc
     3601 tcggtctcga gggtcgcgcg gccggtgcgg ccgctgatgt cgacgatccg tcccgtcgtg
     3661 cccagggtcc gcgagcgcgc ccagaggccg acgcgcggct gcggcacctc gggcgcggcc
     3721 gcgtccgtcg tctcggcctc ggcgtcagca ccacgggccg gtggacgcgc cggcggagcc
     3781 tccgagcggg cgcgcgccgc gcggcggccc agctcctcga gggcggcggc gttggtgctc
     3841 ccacgtcctc ctgccaggtt gcgctggcgc gtcatctcgc gccgcagctc ggccacctcg
     3901 cgctcggcgc gtgccagcat gtcatccgcg gcgcggcgcg catcggtcag gatctctccc
     3961 gcctcccgcc gggcccgcga cacgccggcg cgggcgcgct cccgctcggc ggtcgcctgc
     4021 gaccgttcgc gggtggcggc ctcgagggcc tcctgctgct gctcgcgcgc ctgggcgatc
     4081 gcggccaggg tctcctccat cgtgacgtgc tcggccgaga tgcgggcgcg cgcatcggcc
     4141 aggatgccgg gcgacagacc caaccgctcg gcgatggcga acgcctggct cttgccgggc
     4201 agcccgatgc tgaggcggta cgtcggacgc agggtcgtga cgtcgaactc gaccgccgcg
     4261 ttcgtcacgc cggggtgctc ggtggcgaag gttttcagct cggcgtagtg cgtcgtggcg
     4321 gcgaccatcg atcccgcggc cagcagctgc gacacgatgg ccatggccag cgccgacccc
     4381 tcggtcggat cggtccccgc cccgacctcg tccagcagca ccagcgtatc gggaccggcc
     4441 gctgccacga agcgcaccac gttgcgcaga tggctggaga aggtcgacag cgactgcgcg
     4501 atactctgct cgtcgccgat gtcggccatg acccggcgga acaccggcag gcgcgcccct
     4561 tcggcggccg ggacgcggag gccggcctgg tgcatcagcg cgagcagccc cagggtcttg
     4621 agcgagacgg tcttgccgcc ggtgttcgga ccggtcacca ccagcgcgcg gtagccgaag
     4681 cgctcaccca gccgcaggtc gatcggcacg gccgcgggcc ccagcagcgg gtgacgggcc
     4741 gacagcagct ccacggcatc ggccatcacc gcggggcgga cgccgtccat ctgctcgccc
     4801 aggcgcgcgc gagccatcca gtggtcggca cgggccaggg ccagcaggct ggcgcgcagc
     4861 gcatcggccc gtgcctcgac cccgcgggac agctcgtcca ggatgcggtc ctcctcgcgc
     4921 tgctccgcga gcgtcgcctc cgtccaagtg ttgttgagct ccacgacgga cagcggctcg
     4981 atgaacagcg tcgcgcccga cgcgctctgg tcgtggacga tgcccttgac gcggcccttc
     5041 gcctcagcgc ggattgggat gacgtagcgg cccgcacgga cggtgacgat ggcctcgccg
     5101 atgagtccgg ccatcgtcga cgagcgcagc atggcgttga ggcgctcgcg gacgcgatcc
     5161 tgcgccgtgc gcagccgctt ccggatggcg gccagctccg ccgaggcggt atccaggatc
     5221 tcccccgact cgtcgacgct gcgcccgatg cgttgcgcga gctccggcgc ttcgtccagc
     5281 tcgtcgcgga tgcccgcgag atgcggaccc aaccacgccg acaggcgcgc ggcgaatcga
     5341 ccggtcgcgt cgagcgtctc gcgcaggtcg aggagctcct gcgccgtgag gcgcccgccc
     5401 ctggctgcac gctccagcgc gccccgcacg tcgcgcgccc cgccgatgct ggcctgggcc
     5461 tgttcgttca gcaggcggct ggcctcgtcg gtctggtcct gcagcagagc gacgtgcacg
     5521 gcatcgtcga ccggcacggc ctccagcgca agctcgcgcg acggctggaa gctggtgttc
     5581 gtcgccagct gctcgacgat ggccgcgaac tcgagcgcgc ggatggtcgc ggcatcggcg
     5641 gccgcgccgg caggcaggct atcggcggga gacgtggcgg tcatcggcga tccagaagcg
     5701 caggggacgt gccgcccagt cgccggcgta gtcgacgccg atgcgcgggc cgcggtggat
     5761 ggccggcgcc gcgcccacac gaggctcgat cgagaccggg ccggccagca ggtcggcgcc
     5821 gttgagcgat cggtcgacgc ccagcgcctg gcccacgttg ccgggtccag ccgcgaggcg
     5881 gtggcgcggc acggtcgtcc cacgtcgggc ctgcgccagc tcgaggccct cgtcgatctc
     5941 cagcgcccgg atcagcactg cctcgggctt ctcgccaggc ccggcgacaa cgttgaggca
     6001 gtggtgcagg ccgtagatga ggtacacgta caggtagccg ggcggtccga acatcacggc
     6061 gttgcgcgcc gtgcggccgc gtgacgagtg cgcggcacgg tcctccgggc cccggtacgc
     6121 ctcgacctcc acgatccgcc cgccgaccgt gccctcgggt gacgcgtgga ccagccgcgc
     6181 ccccaggagg tccggcgcga gctcggccga gggccgatcg aaccagtggc gctcgatgcc
     6241 ggtcatcggc acggccgcgg ccgccgcgct cacgggaaga gccgcgccac ctcgtcgggg
     6301 acgaacgggc ggaccaggaa gccggcagtc ggcaggaggg tgtcgcgcag gaattgcacg
     6361 atcagcgagt cgttcatggc cttgacgtag gccggtaccc agccgccctc atcggccccg
     6421 gggccgaagg aggtgtcgaa cacgagcagg tggaaggtga tcagcagcgc cacccacagc
     6481 aggccgaaga tcgcgccacc gagctcgtcc agctgcttgg cgatcggcag gcgggtgcgc
     6541 cggtagagcg cccggatcgc gaaccagccg ccgacgacga acgcgaggaa gagcagcacg
     6601 aagatcagca gcttccggcc ttccggcgtg aacgcggtcc agaagcgcag gagatcgacg
     6661 atcggcccgg tgagctgcga ggccaggatg aacgcgacca gcacggcgat gcagttcagg
     6721 acgtatcgga tcatcccctg cgtgaagccg atgaagacgc caacggcgag caccaggatg
     6781 atcaggaggt ccatccagcc gagctgggcg agcacgtcca tgcggtccga actcctctgc
     6841 cttcacgggg tgccgggcgc gcggacgcgg ctcgggcgtg cgcaagggta gcagccgcgc
     6901 cactggccgg acaaggcgac gacggtccta gcggatctcg gccccgaggt ggtggcgcag
     6961 ggagccgcgg accttgttca gtgccttctc gatggccttc tcctcccccg cctcgcccgg
     7021 ctggaagcgg aaggcgaggg cgtaactcac gcggccccga ccgatctgct cgccgcggta
     7081 cacgtcgaac agccgaagtt cctcgagcag ggggccggcc gccatgcgcg cgacgcgcag
     7141 cagctccccg accggcgtcg cgtcgtcgag cacgacggcc aggtcgcggt cgaccggctg
     7201 cgcgcccggc accggtcgga tgcggtgctc cggctggacg agctccagca gacggccgac
     7261 gtccagcgcc gcgtcgaccg gccgtcccac caggtcccag gcctcgacca cgcgtggatg
     7321 gacctcgccc agcgatccgt acgggcgccc ggccgcgtcg cagatgagcg ccgtccggcc
     7381 cgggtggcgg tgcgcgtgcc gcgacccctc cggctccggc cggtacgtcg gtcgcggcgc
     7441 gccgatcgcg tcgtggagcg cttcgacgac ccccttgatg gcggccacgt ccgccggcag
     7501 cgccgcctcg ccggccgcgg gtgccgccgc ggctccgctg aggatgatcc ccagctccca
     7561 cgactcgtag cggccggttc cggccgtctc ggaggtccgg tcccgcggcg tcggcgcgtc
     7621 cgggtggtac cagtagacct tgccgaggtc gaagagccag gcgtccggcc ggcggcgacg
     7681 aacgttctcc gccagcgcgc cgagcagcga cggagccatc gacggccgca ggatcgagtg
     7741 ctcctcgctg agcgggttat agagccgcac cagctccggg tcatcggccg gtagcgccgt
     7801 gcgcgacagg tccgccggac cgatgagcgc gtgcgtgacg acctcgtcga ggcccaggcc
     7861 ggccaggatg cgacgcaccc ggtgccgcgg gccgctcgga tctggccgat acggcggcag
     7921 cgccgcgacg ggcagccgac ccgggatgcg gtcgtatccg tacgcgcgcg ccacctcctc
     7981 ggcgacgtcg gcctcggcca cgacgtccag gcgctgcggc gggacggcga tctccagcgc
     8041 gtcaccatca ccggagacgt cgaagcccag cggttcgagc agctggcgca ccgccgccgg
     8101 cgccagcggc accccgagca gcctcgccat gcgctcgacc gatgcgccca cgacgcgtcg
     8161 aggatgcggg cccgggtcgt tgtccacgat cccgcgcgcg acccgtgcgc cggtgatctc
     8221 cccgatgagc tgcgccgcgc gatcggcggc gaagcgcggc aggtccgggc cgat
//
LOCUS       DASTYP010000045        12895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1751633, whole genome shotgun sequence.
ACCESSION   DASTYP010000045 DASTYP010000000
VERSION     DASTYP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751633"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1926
                     /locus_tag="VFV59_02310"
     CDS             313..1926
                     /locus_tag="VFV59_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5324692.1"
                     /translation="MQQPHPRWWQTGVVYEIYPRSFADANGDGVGDLAGITSRLDYLA
                     WLGVDAIWIAPFYPSPMADFGYDVADYADVDPRFGTLDDFDALLAAAHERGIRVLIDY
                     VPNHTSDRHPWFVESRSSRDNPKADWYYWRDAGPNGELPNNWISMFAGPSWGWDEQRG
                     QYYLHTFLPEQPDLNWRNPATRDAMFDVARFWLDRGVDGFRIDVAPMVMKDPELRDNP
                     PNPNRGEWHRLGSWSEQIHLNDHNHPEMHDLYRDFRRLLESYPGDRVSIGELHHPDFD
                     TWAAYYGERQDEIHVPFNFHLTFSDWNAAAVRRAVEGVQGALPPGAWASWVLGNHDQP
                     RFASPHRAGRDQAKVGMLLLLTLRGTPTIYYGEEIGMVDVPVPTEAAQDPLERREPGR
                     GRDPERSPMQWDASPNAGFAAAVAKPWLPIAADADTVNVAGQSEDRHSILTLTRDLIR
                     LRREHPVLHRGDFERFGPTPDGVFAFRRMGEEGQLNVVLNLTGEPRQVPDGGPGRILV
                     GTDRSREGTRAGRVLELAPDEGVVVECGR"
     gene            complement(1936..2742)
                     /locus_tag="VFV59_02315"
     CDS             complement(1936..2742)
                     /locus_tag="VFV59_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5324693.1"
                     /translation="MKFGVILTGGPVHEQIELARVAEAAGWDGVFTWDGIHVGDSMEV
                     HDPWVLMSAFAMATERVRIGAIIQPLARRRPWKVARESVTLDHVSSGRFVLCVGLGAT
                     DDSGWARVGEADDRRTRAEKLDETLAILDGLWSGEAFGYEGRHYRFPPMAFRPRPVQR
                     PRIPVWVVGAWPSERSMARAARWDGLLPYVPGGAVTPDAVAQMRTWITERRDSRDGYD
                     IVVEGISRPGDTTEPRRYAEAGATWWIESNWESFDPAATRRRIDAGPPRP"
     gene            complement(2767..3192)
                     /locus_tag="VFV59_02320"
     CDS             complement(2767..3192)
                     /locus_tag="VFV59_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5324694.1"
                     /translation="MASRLNPYLSFPGNAREAMEFYREVLGGELTVTTFGEGGLPHQP
                     GEADKIMHAQLETPAGYTLMGADSVSGSPDTPASGVQISLSGDADADAELSGYFEKLT
                     ARGTVVEPLVTAPWGDKFGMVTDPYGILWMVNIAGARPA"
     gene            complement(3273..4637)
                     /locus_tag="VFV59_02325"
     CDS             complement(3273..4637)
                     /locus_tag="VFV59_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU5324695.1"
                     /translation="MGYAVTNPATGETLATYDTITDTELEAAIAAADEAYRSWSKSTT
                     VDERAALIRRVAELHRERRQELAEIIVREMGKPIAQALSEVDFAADIYEYYANHSPDF
                     LKDEPIELLAGEGSAVIRRASLGVLLGVMPWNFPYYQVARFAGPNLIIGNTILLKHAG
                     QCPESAAAMEQMFLDAGFPAGAYVNLYAGHDQIERIIADPRVQGVSLTGSERAGAQVA
                     EIAGRHLKKVVLELGGSDPFILLSTNDLDAAAQAAVDARLDNSGQSCNAAKRFIVADD
                     LYEPFLETFTAKLAAVEAADPTSADAALGPLSSLEAAERLDAQVKRAVEHGARLVHGG
                     GRDGAFFQTTVLTDVTPDNPASKEEFFGPVAQVYRAKDEADAVRIANDIPYGLGSYVY
                     TTDEEQALRVADAIEAGMVFVNVVLADGAELPFGGIKRSGTGREMGRMGADEFTNKKL
                     IRIG"
     gene            complement(4667..5236)
                     /locus_tag="VFV59_02330"
     CDS             complement(4667..5236)
                     /locus_tag="VFV59_02330"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU5324696.1"
                     /translation="MRIYTRKGDGGTTGLLYGGDRISKAALRTDAYGTTDEAVSALGI
                     ARAAIGEVTDRTEARLAELILRLQRELFVVGAEIATHLDRRERLTDGTTRVTAEMVTA
                     LEGEIDELEGLGEQPKEFVLPGETMTGAGLDLARTTVRRAERRCVALADAGELPDSQV
                     VPYLNRLADLLFVMARVADGGYRPVRTST"
     gene            complement(5287..5577)
                     /locus_tag="VFV59_02335"
     CDS             complement(5287..5577)
                     /locus_tag="VFV59_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEU5324697.1"
                     /translation="MTIDQNLGKVDEVMTALYDIYDPEIGMSIVDLDLIKAVEIGTDG
                     APTEVKMVLTTPFCPWAGELIQNVKTKTEEVVGPPVKVTLLAERWEPPPGLF"
     gene            5763..6308
                     /locus_tag="VFV59_02340"
     CDS             5763..6308
                     /locus_tag="VFV59_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEU5324698.1"
                     /translation="MRKPLLILGLVGLLTTALVAPAAAARPADAGRPTLLSRALAINE
                     QTGEFSTLISLASQYPDIVTALQGKTQLTLFAPTDAAFADLFAFLGTLGVQPSDLTAD
                     QIRTVLLYHVTAGRWSADRLADTSSISMLSGESASVSANGGVAINASNVIIADVAASN
                     GFIHAVDAVLVPPTILAALGL"
     gene            complement(6386..7111)
                     /locus_tag="VFV59_02345"
     CDS             complement(6386..7111)
                     /locus_tag="VFV59_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU5324699.1"
                     /translation="MSHPIAPGGLAAVLAHPDDESFGCGGALARAHDDGATTRLLVVT
                     RGEAGTPDGTPDPVFGDRREAELICAARTIGLDEVSLLDGYADGGIADQPFETLVDEI
                     AAWLAERRPAAVITFGPHGVTGHPDHIVVGNATRWAVERLSDSGIAPNAVYTISPIFD
                     PGGNRYDLSPEEQAATHRIDITAVAQRKLDALACHGSQADTADPISALRSAIAGGGPI
                     YEGYQRVRPIVPAPHPRFDTSLL"
     gene            complement(7121..7480)
                     /locus_tag="VFV59_02350"
     CDS             complement(7121..7480)
                     /locus_tag="VFV59_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324700.1"
                     /translation="MKSRSPVMSRRAFLAATDGLIATGERLVDDPDWDRFAVWLLESD
                     ALLERVWGRMDRYHLAWLNVGRGSAPPGSALDQEGTRRFIAEVASGKLAVLRTMRTSV
                     ERQGWTLLSDDEVPDET"
     gene            7553..9211
                     /locus_tag="VFV59_02355"
     CDS             7553..9211
                     /locus_tag="VFV59_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEU5324701.1"
                     /translation="MGLRRPLDPLRAAVPPAAVLLAATVVVALSGAAAPSLEAIRTSL
                     AVALLVTAASATLVTLRRGLRHGRVADLARAGSAASLAGAAVASLGSAVTVAPALLAA
                     SAFALLAGVGARDDAMVLRGRWRVAAAVAAAVVAEVAVVAGLLPPLADAVRATEPVLL
                     GVAVGAAGTGMLLAAGRRDAGVAAIGMVGIAPLALALGDGAASHVLALGAVTAAELLV
                     LAGPAHETPSGASADADLPRLLDHVPDAVLRFDGRLRLQAWNAAAADLVGLDAGSTGA
                     RLEDLLGIPVGDLPSSAATQSTLEGVGGLAITLHRDGEGVLAVVTQPAVIDDSADRLG
                     RELRATIEELLRARRTIELQRTELERASTVDPLTGVASRAAILERLELEVAEARRYRH
                     PVAIVLLDVDGFGEVNHRVGIDHGDTILREVALRIRLRVRQADALGRSGSDGFLAILP
                     HTDEAGAATFADALRQRASQRPVSVGDDQLRVTLSAGVAVMRPGEELDVDGLLERADE
                     ALESARRAGGDRIALDRLHGLARLEGGASRGASAPEETAQDSGA"
     gene            9283..10224
                     /gene="thyX"
                     /locus_tag="VFV59_02360"
     CDS             9283..10224
                     /gene="thyX"
                     /locus_tag="VFV59_02360"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU5324702.1"
                     /translation="MPPTSPALSAKLIFPTKEIVEEYYLPIIYTACRTCYSEQVPEQI
                     WDKAVSRQVADEKQQSLVRKVMESGHGSTIEHVNFTFAISGVTRTLTHQLVRHRAGTA
                     FDQQSQRYVSFKKRDNFTIPDSITKGDLPDDLAGRFQQAIDDNLDLYGQLLQAEVPAE
                     DARFIFPNAMQTNLIMTVNLRQLIHMSGLRLCTMAQWEIRQLFKQIRHEIFRVSPFFG
                     SFLAPKCVPLGYCDEMGNRDEHCRIRPHRDTVMAVWEAYRSGELAETDGPVVPETSPF
                     RPKPRRVPLPISEESVRPSVGEPVGAQLGQASDSDRV"
     gene            10264..11586
                     /locus_tag="VFV59_02365"
     CDS             10264..11586
                     /locus_tag="VFV59_02365"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEU5324703.1"
                     /translation="MRLHFAHRWLAVRASFWFVPGLMVLGAALLAGIALTIDSAIAGS
                     DMPPWIYAGGADGARALLSTVAGSMVTVAGVGFSVTIVALVLASTQFGPRLLTVFMRD
                     LTSQVTLGSFTSTFTYCVLVLRTVTGTADQANPFVPQLAITIAIVMTLLSIAALVYFF
                     HHVAVSIQAPKVVSAVARDLNHGIERLYPHRIGQPGPEPEPEAIPQPMTDAVITAEAS
                     GYVQVVDDERLLQLARDHDLVVRLMTRPGLFVVDGHPVIVARPASRISRPVAAALHDT
                     IIVGDVRTAEDDIEFSIRQLVEVALRALSPAINDPFTAMAAIDWLGAALARLAREQFP
                     SRYRYDEDGRLRVVADVSTFGGVTHTIFSRIRHYGGSSPVVLNRLLESIVAFGPHVSD
                     EADRQLVRDEMEAVLQTGRTEISSEADLRELERRHRAALAALGEVVAD"
     gene            complement(11583..12017)
                     /locus_tag="VFV59_02370"
     CDS             complement(11583..12017)
                     /locus_tag="VFV59_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324704.1"
                     /translation="MTDALQTGVPPDGMPSPDLVPPQIARSGDPFAALRIVHFASRLP
                     RNRTLQLRDVVAALNAEFLDWFFSEKVVIAELVQLQANWAISFHGDDRIVLDRNERGH
                     TLLITDSTKMGSFLVNEARRLAEACAEELRRFTLGDGVSPDN"
     gene            complement(12103..>12895)
                     /locus_tag="VFV59_02375"
     CDS             complement(12103..>12895)
                     /locus_tag="VFV59_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEU5324705.1"
                     /translation="TLSRLAGMLAGTALVAALAVPVAAADDAMVRVVHASPDAPNVDV
                     WVDGETVLTDVPFTAVSDYLSLPAGTYNIQVTATGDTTPVIEADLAFEAGTAYTVAAY
                     GRLADITAAVFTDDTTVASGQAKLRAIHLSPSAPADVDIAVQGGDVVVPGLAYPEASG
                     YLTLDAGDYPLEIRAAGDTAAALQFDAALAADTNVTAFAMDAEDGVQVITAADATGGA
                     MPDTALAAPVATGASTTVIAGIALLGLAALGAMRVLATRRVTTER"
BASE COUNT         1819 a         4587 c         4596 g         1893 t
ORIGIN      
        1 cgaaacaggc gctcggcttc acggggatcg agcggcgcag tccgcccggc gggttgctcg
       61 cccgccgttc cgggatcggt ccgggcaggg ccggcgctcc tgaggtccgt cacgccccga
      121 tcgtagcagg ggcaggtgga acccgatgag cccggtcgcc cggggaccgc ggaggatccc
      181 taggcgaccg agttcgcgcg ccgctggaga tcggctcgcg gggcagcacg ctcgccgggc
      241 gcggcgagct cggcagcgtc ctcggcggcg ccggaggcgc cggaagcggc gcatcggtct
      301 agcctgtgcg gcatgcagca gccgcaccct cgctggtggc agaccggcgt cgtctacgag
      361 atctacccgc gctcgttcgc cgatgccaac ggcgacggcg tcggagacct ggcggggatc
      421 accagccgtc tcgactacct ggcgtggctc ggcgtggatg cgatctggat cgcgccgttc
      481 tatccctcgc cgatggccga cttcggctac gacgtggccg actacgccga cgtcgacccg
      541 cgcttcggga cgctggacga cttcgacgcc ctgctggccg cggcgcacga gcgcggcatc
      601 cgcgtcctga tcgactacgt cccgaaccac accagcgatc ggcacccgtg gttcgtcgag
      661 tcgcgctcct cgcgcgacaa cccgaaggcc gattggtact actggcgcga cgccgggccc
      721 aacggcgagc tgcccaacaa ctggatcagc atgttcgccg ggccttcgtg ggggtgggac
      781 gagcagcgcg gccagtacta cctgcacacg ttcctgcccg agcagccgga cctcaactgg
      841 cgcaatccgg cgacgcggga cgccatgttc gacgtcgcgc gcttctggct cgaccggggc
      901 gtggacggct tccggatcga cgtggccccg atggtgatga aggatcccga gctgcgggac
      961 aacccgccca accccaaccg cggcgagtgg catcggctcg gctcatggtc cgagcagatc
     1021 cacctgaacg accacaacca tccggagatg cacgacctgt atcgggactt ccggcgtctg
     1081 ctcgagtcgt atcccggtga ccgggtcagc atcggcgagc tgcaccatcc cgacttcgac
     1141 acctgggccg cgtactacgg cgagcggcag gacgagatcc acgtcccgtt caacttccac
     1201 ctcaccttca gcgactggaa cgccgcggcc gtgcggcggg cggtcgaggg cgtgcagggg
     1261 gcgctgccgc ccggggcgtg ggccagctgg gtgctcggca accacgatca gccgcgattc
     1321 gcctcgcccc accgggcggg acgcgaccag gcgaaggtcg gcatgctgct gctgctcacc
     1381 ctgcgcggca cacccaccat ctactacggc gaggagatcg ggatggtgga cgttccggtc
     1441 ccgaccgagg cggcccagga cccgctggag cgacgcgagc ccgggcgcgg gcgcgatccg
     1501 gagcgttcac cgatgcagtg ggacgcctcg cccaacgccg gattcgcggc ggccgtcgcc
     1561 aagccctggc tgcccattgc cgccgatgcc gacacggtca acgtggcggg tcagtcggag
     1621 gatcggcact cgatcctgac cctcacccgc gacctcatcc gcctccgccg cgagcacccg
     1681 gtcctccacc gcggggactt cgagcgcttc ggtcccacgc ccgacggtgt cttcgccttt
     1741 cggcgcatgg gcgaggaggg gcagctcaat gtcgtgctga acctgaccgg ggaaccgcgg
     1801 caggtgccgg acggcggacc gggacggatc ctggtcggca ccgatcgcag ccgcgagggg
     1861 acgcgggcgg gtcgggtgct cgagctggcg ccggacgagg gcgtggtggt ggagtgcggc
     1921 cgctgaggcg cccgcctagg gacgcggcgg cccggcatcg atacgccggc gcgtggcggc
     1981 cggatcgaag ctctcccagt tcgactcgat ccaccaggtg gcaccagcct cggcgtagcg
     2041 acgcggctcc gtggtgtcgc ccggcctgct gattccctcg acgacgatgt cgtagccatc
     2101 gcgcgagtcc cggcgctccg tgatccacgt ccgcatctgc gccacggcgt cgggcgtcac
     2161 ggcgccgccc ggcacgtagg gcaggaggcc gtcccagcgg gcggcacgcg ccatggaacg
     2221 ttccgacggc cacgcgccga ccacccagac cgggatgcgc gggcgctgga cgggacgtgg
     2281 tcggaacgcc atcggcggga accgatagtg gcgcccctcg tatccgaacg cctcgccgct
     2341 ccacaggccg tcgaggatgg ccagggtctc gtccagcttc tcggcgcgcg tgcggcggtc
     2401 gtccgcctcg ccgacgcgcg cccacccgct gtcgtcggtt gcgccgaggc ccacgcacag
     2461 gacgaagcgg ccgctcgaga cgtggtcgag ggtcacggac tcgcgcgcga ccttccaggg
     2521 gcggcgtcgg gcgaggggct ggatgatcgc gccgatgcgc acccgctccg tcgccatggc
     2581 gaaggcggac atgaggaccc acggatcgtg gacctccatc gagtccccga cgtggatgcc
     2641 gtcccaggtg aagacgccgt cccaacccgc ggcctcggcg acccgggcga gctcgatctg
     2701 ctcgtggacc ggaccgccgg tcaggatgac gccgaacttc atcggccgat gatcggcagg
     2761 cgccgtctac gcagggcgtg cgccggcgat gttcaccatc cagaggatgc cgtacgggtc
     2821 ggtgaccatc ccgaacttgt cgccccacgg cgccgtgacg agcggctcga ccacggtgcc
     2881 acgtgccgtg agcttctcga agtagccgga gagctccgca tcggcgtcgg catcgccact
     2941 cagcgagatc tgcactccgc tcgccggcgt gtccggcgag ccgctcacgg agtcggcacc
     3001 catcagcgtg tacccggcag gcgtctcgag ctgcgcgtgc atgatcttgt cggcttcgcc
     3061 aggctggtgc gggagcccgc cctcgccgaa ggtggtgacg gtcagctcac cgccgagcac
     3121 ctcgcggtag aactccatcg cctcccgggc gttgccgggg aagctcaggt aggggttgag
     3181 ccgggatgcc atcgtctgct cctccgtgac tggtgatcgg acaccggtac agacgatagg
     3241 cgccccggca actcatcggt tcaggtcagc gatcagccga tccggatcag cttcttgttg
     3301 gtgaactcgt cggcgcccat gcggcccatc tcgcggccgg tgccggaacg cttgatgccg
     3361 ccgaacggca gctccgcgcc gtcggcgagc acgacgttca cgaacaccat cccggcctcg
     3421 atcgcatcgg cgacgcgcag cgcctgctcc tcgtcggtgg tgtagacgta cgaccccagg
     3481 ccatagggga tgtcgttcgc gatccgcacg gcgtcggcct cgtccttcgc gcggtacacc
     3541 tgagcgaccg gtccgaagaa ctcctccttg gaggcggggt tgtccggcgt cacatcggtc
     3601 agcacggtcg tctggaagaa cgcgccgtcg cggccgccac catggaccag ccgggccccg
     3661 tgctcgaccg cgcgcttgac ctgcgcgtcc aggcgctcgg cagcctccag cgaggagagc
     3721 gggccgagcg cggcatcggc cgacgtcggg tccgcggcct cgaccgcggc gagcttcgcg
     3781 gtgaacgtct cgaggaacgg ctcgtagagg tcgtcggcca cgatgaagcg cttggccgcg
     3841 ttgcaggact ggccgctgtt gtccaggcgc gcgtcgaccg cggcctgggc ggcggcgtcc
     3901 aggtcgttgg tcgagagcag gatgaagggg tcggagcccc cgagctcgag cacgaccttc
     3961 ttcagatgcc gaccggcgat ctccgcaacc tgggcgccgg cacgctcgga accggtgagc
     4021 gagacgccct gcacgcgggg gtccgcgatg atccgctcga tctggtcgtg cccggcatag
     4081 aggttcacgt acgcgccggc cgggaagccc gcgtccagga acatctgctc catggcggcg
     4141 gccgactcgg gacactggcc ggcgtgcttc agcaggatcg tgttgccgat gatgaggttc
     4201 ggcccggcga atcgggcgac ctggtagtag gggaagttcc agggcatgac gccgaggagg
     4261 accccgagcg acgcgcgacg gatgaccgcc gagccttcgc cggcgagcag ctcgatcggc
     4321 tcgtccttca ggaagtccgg cgagtggttg gcgtagtact cgtagatgtc cgcggcgaag
     4381 tcgacctcgg agagggcttg ggcgatcggc ttgcccatct cccgcacgat gatctccgcc
     4441 agctcctggc ggcgctcgcg atgcagctcg gcgacgcggc ggatgagggc cgcgcgctcg
     4501 tcgacggtgg tggacttcga ccaggaccgg tacgcctcgt cggccgccgc gatcgcggcc
     4561 tcgagctcgg tgtcggtgat ggtgtcgtac gtggcgaggg tctccccggt cgcggggttg
     4621 gtgacggcat aacccatcgt gtgattctcc tccgagaagc cgattactac gtgctggttc
     4681 gaaccggccg atacccgccg tcggcgacgc gcgccatgac gaacagcagg tcggcgaggc
     4741 ggttcaggta gggcacgacc tgggaatcgg gcagctcccc cgcatcggcc agggccacgc
     4801 aacgccgctc ggctcgacgc acggtcgtgc gcgccaggtc cagcccggcg ccggtcatcg
     4861 tctcgcccgg aaggacgaac tccttcggct gctcgccgag cccctccagt tcgtcgatct
     4921 ccccctcgag ggcggtcacc atctcggccg tgacgcgcgt cgtgccatcg gtcaggcgct
     4981 cccgacgatc gagatgcgtg gcgatctcgg ccccgaccac gaagagctcg cgctggaggc
     5041 gcaggatcag ctcggccagg cgggcctccg tgcgatcggt gacctccccg atcgcggcac
     5101 gcgcgatccc gagcgcggag acggcctcat cggtggtgcc gtaggcatcg gtccgcaggg
     5161 cggccttgct gatgcgatcg ccgccgtaga gcagcccggt ggtgcctccg tcgcccttgc
     5221 gggtgtagat gcgcatgcgc gcatgctacc ggggggatat gcggcgcgac gacgagcggc
     5281 tgcgacctag aagaggcccg gcggcggctc ccaccgctcg gccagcagcg tgaccttcac
     5341 gggcggcccc acgacctctt cggtcttggt cttcacgttc tggatcagct cgccggccca
     5401 cgggcaaaac ggcgtcgtga ggaccatctt cacctcggtc ggcgcgccat cggtcccgat
     5461 ctcgacggcc ttgatcaggt cgagatccac gatgctcatc ccgatctccg gatcgtagat
     5521 gtcgtacagc gcggtcatca cctcgtcgac cttgccgagg ttctggtcga tcgtcatggc
     5581 ccaaggatac gcggatgcgc gagggagtgc ccgagggcca cccgtcggat gtcccgcctc
     5641 ggtcgaccag gttcccctcg tcgagcgcgg ggagcgagtg tggcgcgcac tcatccgagt
     5701 accggcgagg accgtaggag tgggcacaga gcttccccca acgacacaag gaaccagtac
     5761 cgatgcgcaa gccccttctc atcctcggcc tcgtcggcct gctgacgacg gcgctcgtcg
     5821 ccccggccgc ggcggctcgt cccgccgatg ccggccgtcc gaccctgctc agccgcgcgc
     5881 tggcaatcaa cgagcagacc ggcgagttca gcacgctcat ctcgctggcg agccagtacc
     5941 ccgacatcgt gaccgccctc cagggcaaga cccagctcac gctgttcgcc ccgaccgacg
     6001 ccgccttcgc cgacctcttc gcgttcctcg ggaccctcgg cgtccagccg agtgacctga
     6061 ccgcggacca gatcaggacg gtgctgctgt accacgtgac cgctggccgc tggtcggcag
     6121 atcgcctggc cgacacgagc tcgatctcga tgctgtccgg cgagtccgcg tcggtcagcg
     6181 ccaacggcgg cgtggcgatc aacgcctcga acgtgatcat cgccgacgtc gcggcgagca
     6241 acgggttcat ccacgccgtc gacgcggtgc tggtcccgcc gaccatcctg gccgccctgg
     6301 gcctgtagac gacaccctcc ctcccgcgca cgagccggtc ccgccagggg ccggctcgtg
     6361 gcgtatcggt ggcggcaggc gagcgctaga gcagggacgt gtcgaagcgc ggatgcgggg
     6421 ccgggacgat cggccggacg cgctggtaac cctcgtagat cgggccgccg ccggcgatgg
     6481 cgctgcgcag cgccgagatc ggatccgccg tatcggcctg gctgccgtgg caggcgagcg
     6541 cgtcgagctt gcgctgggcg accgcggtga tgtcgatgcg gtgggtggcc gcctgctcct
     6601 ccggcgagag gtcgtaccgg ttcccgccgg gatcgaagat cggggagatg gtgtagaccg
     6661 cgttcggcgc gatcccggag tccgagagcc gctcgaccgc ccagcgggtc gcgttgccga
     6721 cgacgatgtg atccgggtga cccgtgacgc cgtgcggccc gaaggtgatg accgccgcgg
     6781 gccggcgttc ggcgagccac gccgcgatct cgtcgacgag cgtctcgaac ggctggtccg
     6841 cgatcccgcc gtcggcgtag ccgtccagca gcgacacctc gtccagcccg atggttcggg
     6901 cggcgcagat gagctccgcc tcgcggcggt cgccgaacac cgggtcgggc gtcccgtccg
     6961 gggtgccggc ctcgccgcgg gtgacgacca ggagccgcgt cgtggcgcca tcgtcgtggg
     7021 cgcgcgccag cgcgccgccg cagccgaagc tctcgtcgtc agggtgggcc aggacggctg
     7081 ccaggccgcc cggggcgatc ggatgactca ccgatgctcc ttacgtctcg tcaggcacct
     7141 cgtcgtcgga cagcagggtc cagccctgcc gctcgacgga ggtcctcatc gtacgcagga
     7201 ccgccagctt cccgctggcg acctccgcaa tgaagcgccg cgtgccctct tggtccaggg
     7261 cgctgccggg cggggcgctg ccgcggccga cgttcagcca ggcgaggtgg tagcgatcca
     7321 tccggcccca cacgcgctcg aggagcgcgt cggactcgag cagccacacg gcgaaccggt
     7381 cccagtcggg gtcgtcgacc aggcgttccc ccgtcgcgat cagcccatcg gtcgcggcca
     7441 ggaacgcgcg gcgcgacatg acgggcgatc gggacttcac ggcgcccgag tatagggacc
     7501 ggcgttcgta ccattgccgg ggcgacgatc ggcgggcgac gatcgggcgc atatgggtct
     7561 ccgccgtccg ctcgatccgc tgcgcgccgc ggtgccgccg gcggccgtgc tcctggccgc
     7621 gacggtggtc gtggcgctgt ccggcgccgc ggcaccgagc ctcgaggcga tccgcacgtc
     7681 gctcgccgtg gcgctgctcg tcacggcggc ctccgcgacg ctcgtcaccc tgcgacgggg
     7741 cctccggcac gggcgcgtcg ccgatctcgc gcgggccgga tccgcggcat cgctggccgg
     7801 cgccgcggtc gcgtcgctgg gttcagccgt caccgtcgct ccggcgctgc tggccgcgtc
     7861 ggcgttcgcg ctcctcgccg gggtgggcgc acgcgacgac gccatggtcc ttcgcggccg
     7921 ctggcgcgtc gcggctgccg tggctgccgc ggtggtcgcg gaggtcgcgg tggtggccgg
     7981 gctgctgccc cctctggccg atgcggtccg cgccaccgag ccggtcctcc tcggcgtcgc
     8041 ggtcggggcg gccggcaccg gcatgctcct cgccgccgga cggcgggacg cgggcgtcgc
     8101 ggccatcggc atggtcggga tcgctccgct cgcgctggcg ctcggggacg gggcggccag
     8161 ccacgtgctg gccctgggcg cggtcaccgc tgccgagctg ctcgtcctcg ccggcccggc
     8221 gcacgaaacg ccctccggcg cgtcggccga cgccgacctg ccacgtctgc tggaccacgt
     8281 ccctgacgcg gtcctgcgct tcgacggccg cctgcggctg caggcgtgga acgcggccgc
     8341 ggccgacctg gtcgggctcg atgccggatc gacgggcgcg cgcctcgagg atctgctcgg
     8401 catcccggtc ggggacctgc cgtcatcggc agcgacgcag tcgaccctcg agggcgtggg
     8461 cggcctggcg atcacgctgc accgcgacgg cgaaggggtg ctggccgtcg tgacgcagcc
     8521 cgcggtgatc gacgactcgg cggaccggct cgggcgcgag ctgcgtgcga ccatcgagga
     8581 gctgctgcgt gcgcggcgca cgatcgagct gcagcgcacg gagctggagc gcgcctcgac
     8641 cgtcgacccg ctgaccgggg tcgcgagccg ggcggccatc ctggagcgcc tcgagctgga
     8701 ggtggccgag gcgcgacgct accgccaccc cgtggccatc gtgctgctgg acgtggacgg
     8761 attcggggag gtcaaccacc gcgtcggcat cgatcacggc gacacgatcc tgcgcgaggt
     8821 ggcgctccgc atccggttgc gcgtccgcca ggcggatgcg ctcggacgat ccggcagcga
     8881 cggcttcctg gccatcctgc cccacaccga cgaggccggc gccgcgacct tcgccgatgc
     8941 gctccggcag cgcgcgagcc agcggcccgt cagcgtcggc gacgatcagt tgcgcgtgac
     9001 gctgagcgcc ggggtggcgg tcatgcgccc gggtgaggag ctcgacgtcg acggcctcct
     9061 cgagcgcgcc gacgaggcgc tcgagagcgc ccgccgcgcg ggtggcgacc gcatcgcgct
     9121 ggatcggctg cacgggctgg cgcgactcga gggaggagcg tcccgcggtg catcggctcc
     9181 ggaggaaaca gcacaagata gcggtgcctg acttatccac accgctacat gttgtggata
     9241 acgctcgcca gcggcaggtg tcgactggta cactccgccg caatgccgcc gacctctccc
     9301 gcgctcagcg ccaagctcat cttccccacc aaggagatcg tcgaggagta ctacctcccg
     9361 atcatctaca ccgcctgccg cacctgctac tccgagcagg tcccggagca gatctgggac
     9421 aaggcggtga gccggcaggt cgccgacgag aagcagcaga gcctcgtccg caaggtcatg
     9481 gagtccggcc acggctcgac catcgagcac gtcaacttca ccttcgccat cagcggcgtg
     9541 acgcggacac tgacgcacca gctcgtccgg caccgcgcgg gcacggcctt cgaccagcag
     9601 agccagcgct acgtcagctt caagaagcgc gacaacttca ccatccccga ctcgatcacc
     9661 aaaggcgacc tgcccgacga cctggcgggc cgattccagc aggcgatcga cgacaacctc
     9721 gacctctacg ggcagctgct gcaggccgag gtgcccgccg aggacgcgcg cttcatcttc
     9781 cccaacgcca tgcagaccaa cctgatcatg acggtgaacc tgcgccagct catccacatg
     9841 agcggcctgc ggctgtgcac catggcgcag tgggagatcc ggcagctgtt caagcagatc
     9901 cggcatgaga tcttccgggt cagccccttc ttcggaagct tcctggcgcc gaagtgcgtg
     9961 ccgctgggct actgcgacga gatgggcaac cgcgacgagc actgccgcat ccggccgcac
    10021 cgcgacacgg tcatggcggt ctgggaggcg taccgcagcg gcgagctggc cgagaccgac
    10081 ggaccggtgg tgccggagac gagccccttc cggccgaagc cgcgccgcgt cccgctgccg
    10141 atcagcgagg aatcggttcg cccctccgtg ggcgagccgg tcggcgccca gctcgggcag
    10201 gcgagcgact ccgaccgggt ctgaccggcg ctacgctccg ggggtgacgc gcacggctcg
    10261 gccatgaggc tccatttcgc ccaccgctgg ctcgcggtgc gcgccagctt ctggttcgtg
    10321 ccgggcctga tggtcctcgg tgccgcgctc ctcgccggga ttgccctgac gatcgactcc
    10381 gccatcgcgg gatcggacat gccgccgtgg atctacgccg gcggcgccga tggtgcgcga
    10441 gcgctgctgt ccacggtcgc cgggtcgatg gtcacggtcg ccggcgtggg gttttccgtc
    10501 acgatcgtcg ccctggtcct cgcgtcgacc cagttcggcc cgcgcctgct cacggtcttc
    10561 atgcgcgacc tcaccagcca ggtcacgctc gggtccttca cgtcgacgtt cacctactgc
    10621 gtcctggtgc ttcgcacggt gaccggaacg gccgaccagg ccaacccgtt cgttccacag
    10681 ctcgccatca ccatcgcgat cgtgatgacg ctgctgagca tcgcggccct cgtgtacttc
    10741 ttccaccacg tcgcggtatc gatccaggcg ccgaaggtcg tctccgccgt ggcccgagat
    10801 ctgaaccacg gcatcgagcg gctctatccg catcgcatcg gtcagcccgg gccggagcca
    10861 gagccggaag ccatcccgca gccgatgacc gacgcggtga tcacggctga ggccagcggc
    10921 tacgtccagg tcgtcgacga cgagcggctc ctccagctcg cgcgggacca cgatctggtg
    10981 gtgcggctca tgacgcggcc ggggctgttc gtcgtcgacg gccacccggt gatcgtcgcg
    11041 aggccggcgt cgcggatcag ccgtccggtg gctgccgccc tgcacgacac catcatcgtc
    11101 ggcgacgtcc gcacggccga ggacgacatc gagttctcga tccgacagct ggtcgaagtc
    11161 gcccttcgtg ccctctcacc ggccatcaac gacccgttca ccgccatggc cgccatcgac
    11221 tggctgggcg ccgcgctggc tcggctcgcg cgcgagcagt tcccgtcgcg ataccgctac
    11281 gacgaggacg gacggttgcg cgtcgtggcc gacgtgtcga ccttcggcgg agtgacgcac
    11341 accatcttca gccggatccg gcattacggc ggatcgagcc cggtcgtcct gaaccggctg
    11401 ctcgagtcca tcgtcgcctt cggcccccac gtgagcgacg aggcggatcg gcagctcgtg
    11461 cgggacgaga tggaagccgt gctccagacg ggacgaacgg agatctcgag cgaggcagac
    11521 ctgcgggagc tcgagcgtcg tcatcgcgcg gcactggcgg cgctcggcga ggtggtcgcc
    11581 gactagttgt cgggggagac gccatcaccg agggtgaagc gacgcagctc ctcggcgcac
    11641 gcctcggcca ggcgtcgcgc ctcgttgacc aggaagctgc ccatcttcgt ggagtcggtg
    11701 atcagcagcg tgtggccccg ctcgttgcgg tcgagcacga tccggtcgtc gccgtggaag
    11761 ctgatcgccc agttcgcctg cagctggacc agctcggcga tcaccacctt ctcgctgaag
    11821 aaccaatcga ggaactcggc gttgagggcc gccaccacgt cacgcagctg gagcgtgcgg
    11881 ttgcggggta gtcgcgacgc gaagtgcacg atccgcagcg cggcgaaggg gtcgccgctg
    11941 cgcgcgatct gcggggggac gaggtcgggc gacggcatcc cgtcgggagg cacgccggtc
    12001 tggagggcat cggtcaccgc gcgagtatcc ccggcgaggt gaggcgcggc aaacgggccg
    12061 ccgtccggtg agggacgacg gcccgttgca gcgcggcgag ggtcagcgct cggtcgtgac
    12121 ccgtcgcgtg gccaggacgc gcatcgcgcc gagggccgcg aggcccagca gggcgatgcc
    12181 cgcgatgacg gtggtggaag cacccgtcgc caccggggcc gccagggcgg tatccggcat
    12241 ggcaccaccc gtggcatcgg ccgcggtgat gacctggacg ccgtcctcgg catccatggc
    12301 gaacgccgtg acgttcgtgt ccgcggccag tgccgcgtcg aactggagcg cggcggccgt
    12361 gtccccggcg gcccggatct cgagcgggta gtcgcccgcg tcgagggtca ggtagccgct
    12421 cgcctcgggg taggcgagcc cgggcacgac cacgtcgcca ccctggacgg cgatgtcgac
    12481 gtcggcgggc gcgctgggcg acaggtggat ggcgcggagc ttggcctggc cgctggcgac
    12541 ggtcgtgtcg tcggtgaaga ccgcggcggt gatgtcggcc agcctgccgt acgcggccac
    12601 cgtgtacgcc gtgccggcct cgaaggccag gtcggcttcg atcaccgggg tggtgtcccc
    12661 cgtggctgtg acctggatgt tgtacgtccc ggccggcagc gacaggtagt cgctgaccgc
    12721 ggtgaacggc acgtcggtca ggaccgtctc gccgtcgacc cagacgtcga cgttcggggc
    12781 gtcgggcgag gcgtggacga cgcgcaccat ggcgtcatcg gctgcggcca cgggaaccgc
    12841 gagggcggca accagcgccg tgccggccag catgccggcg aggcgggaga gcgtt
//
LOCUS       DASTYP010000046         5191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1605931, whole genome shotgun sequence.
ACCESSION   DASTYP010000046 DASTYP010000000
VERSION     DASTYP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605931"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFV59_02380"
     CDS             complement(<1..382)
                     /locus_tag="VFV59_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HEU5324706.1"
                     /translation="MLTTALRALFLWLSNRRWLGRVAMGTPLLRRMPLRFVAGTTLDA
                     AVAAVRELNATGATATLDVLGEAVDDRASAERAAQAYVTTIERIAAEGLRSGVSIKLT
                     QMGLDLGVDACLEVIAPVVEAGERH"
     gene            complement(453..782)
                     /locus_tag="VFV59_02385"
     CDS             complement(453..782)
                     /locus_tag="VFV59_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324707.1"
                     /translation="MRVELLYADGDPASMPARQNLVEVLGEDAFETPIQMIAVASDED
                     AAFLEMRGTPTIRIDGADIQPEWDGPVGVQPRDYGEGPVPSKALIRRAVERARGWSHG
                     PIPRRRE"
     gene            911..1510
                     /locus_tag="VFV59_02390"
     CDS             911..1510
                     /locus_tag="VFV59_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324708.1"
                     /translation="MRRLVLVVIAMVAFLSMAAPVAAARPIKESGTFEQALAFSSSCT
                     QQGPNTSCTDAEVDYFSYPDGYTEACVWIFSYTIGRNGQFRPGAQQSGCMPGAVGDVS
                     DDASAATLTATEVQLFDCNQRTCTPGDTVTVSASWSAVGAPMTYTRRETFSDGTCTYR
                     QTITGVQSEATSSFSIGSATYNGDGFLVHEDYAVTARCR"
     gene            complement(1550..1819)
                     /locus_tag="VFV59_02395"
     CDS             complement(1550..1819)
                     /locus_tag="VFV59_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324709.1"
                     /translation="MSPLVWLVIAIVAAAGAYLIGWPAWRSYRSRESRDLNTERYLQW
                     RGRASRPSSGSTREGMTGEERRRVYVAAALAALAIVALIAFFATS"
     gene            complement(1825..2478)
                     /locus_tag="VFV59_02400"
     CDS             complement(1825..2478)
                     /locus_tag="VFV59_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324710.1"
                     /translation="MSEPLTPDGLRILEAQLGRMRSMLYRYYELFFRFVAGGTVAVGL
                     LFIGSFWAPTQAGSLLLPLVILYVGFHASHLFSYVIFARTYAAALERRINRALGDELL
                     VAHRLEEAYFGAPGDPRVVAASLRRPLTMLAAETWHFTMVGAALFAVSLLLANATVQR
                     VGEPWSTFYTPVVIGWALLNGAYLAWHFIGRRDQREVEAILQDAYGAEPMPASVKEG"
     gene            complement(2475..3203)
                     /gene="pgl"
                     /locus_tag="VFV59_02405"
     CDS             complement(2475..3203)
                     /gene="pgl"
                     /locus_tag="VFV59_02405"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU5324711.1"
                     /translation="MTRFTVLPDPSAVAMATADRFVADAVAAIEERRIFRVALSGGST
                     PKQVYPLLLEPQRRDAVDWSRVEFFWGDERAVPPDHPESNFGVAYGMLISQLPNVRQD
                     RIHRMPAEAPDRDAAALSYESELRLAFGARGEEPPAFDLIWLGMGPDGHCASLFPGSQ
                     ALTVMDRWVVANWAPGPSAWRMTLTFPVLNAGRDVLFVVTGADKADAFGDIRAGTSTD
                     PAGRVSGDNVEWFVDAAAAGEDRS"
     gene            complement(3200..4489)
                     /locus_tag="VFV59_02410"
     CDS             complement(3200..4489)
                     /locus_tag="VFV59_02410"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEU5324712.1"
                     /translation="MAQAVTNGGRAATEEQAVHRRLMRFGEAETLVTPMWEERDTSVR
                     AIASHLATLFDLPAGVDDGGDPLVREKGLPHARASVLNLIVTVVDADAADRAVQTLMG
                     LGVRHPSRAIVLVPQRSRGDAPLDARISTHCHETSSGGDRICYEEVILTVRGEAAAHL
                     SGVVAPLLIHDLPTHVWWPGDPPFADPVFAQLVEMADRVVVDSSDFSDLLGGLRRLAG
                     LRRRSGVGDLTWERLSWWQELTAQFFDAPRFRRYLPNLSRLWIRYATAPPGSQRHDED
                     EEVAPGVSAPMAEALLYAGWMATRLGWRRHQTLQPLTDGRTVMRLEGRHEMVDLVIEP
                     AETDTVRPGELLSVRMRSLGETGAAEFIIDRDAEEATVATNADGMTALLRRVRMEVPR
                     EPELLSAQLTLDTADRVYEDALRAAAILLSSAHEAAA"
     gene            complement(4474..>5191)
                     /locus_tag="VFV59_02415"
     CDS             complement(4474..>5191)
                     /locus_tag="VFV59_02415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5324713.1"
                     /translation="PVEFAAEDLRDEKLKVLRGVKPMTPEEVAANTVRGQYVSGWVEG
                     EKVPSYRDEPEVAPDSETETFVALKLAIDSWRWAGVPFYLRTGKAMPSRVTEIAVQFK
                     RAPLALFARAGVPQIDANVLAIRVQPDEGILLRFGAKVPGQGLQIRTVNMDFRYGASF
                     AVDSPDAYETLLLDAMVGDASLFTRDDEVERAWEILDPILNAWAAGEGGPLHFYGAGT
                     WGPPAADELLERDGRSWRKP"
BASE COUNT          775 a         1866 c         1862 g          688 t
ORIGIN      
        1 cgtggcgctc ccccgcctcg acgaccggcg cgatgacctc caggcacgcg tccacgccga
       61 ggtccaggcc catctgagtc agcttgatgc tcacgccact ccgcagccct tcggccgcga
      121 tgcgctcgat ggtcgtcacg taggcctgcg cggcccgctc agccgaggcg cggtcgtcaa
      181 cggcttcgcc aagcacgtcg agggtggccg tggcgccggt agcattgagt tcacggaccg
      241 cggccacggc agcgtccagg gtggtgcccg ccacgaagcg cagcggcatg cggcgcagca
      301 gtggcgtgcc catggccacg cggcccagcc agcggcggtt gctcagccac aggaagaggg
      361 cgcgcagggc ggtggtgagc atcggatgcg agtctagcca ggccgcccgc tggcaggagg
      421 gccgagcggc acggtcggag ccgggacggg agtcactccc ggcggcgcgg aatgggtccg
      481 tgactccagc cgcgggcgcg ctcgacggca cggcggatga gcgccttcga cggcacggga
      541 ccctcgccgt agtcgcgtgg ctggaccccg accggtccgt cccactcggg ctggatgtcg
      601 gccccgtcga tccggatcgt cggcgtgccg cgcatctcga ggaaggccgc gtcctcgtct
      661 gacgccacgg cgatcatctg aatcggagtc tcgaacgcgt cctcgcccag gacctccacc
      721 aggttctggc gggcgggcat cgaggccgga tcgccatcgg cgtacaacaa ctcgacgcgc
      781 atcgcgtcga gtatacggac gctcctcccg accgccgccg cgtgccccga ccgcgcatgg
      841 atggacgtcc gaggcggcgt ccaggccgga cccgagctcc ggtcgacgcc ccgcatggga
      901 ggtcgacgaa atgcgtcgtc tcgtcctcgt ggtcatcgcc atggttgcgt tcctgtccat
      961 ggccgcaccg gtcgccgccg cgcgaccgat caaggagtcg ggcacgttcg agcaggccct
     1021 cgccttcagc tcgtcgtgca cccagcaggg cccgaacacc agctgtaccg atgcggaggt
     1081 cgactacttc agctaccccg acggctacac cgaggcctgc gtctggatct tctcctacac
     1141 catcggtcgg aacgggcagt tccggcccgg cgcgcagcag tcgggctgca tgcctggggc
     1201 cgtcggcgac gtcagcgacg atgcgtcggc cgcgacgctc accgccacgg aggtccagct
     1261 gttcgactgc aaccagcgca cctgcacgcc gggcgacacc gtcacggtct ccgcctcgtg
     1321 gagcgccgtc ggtgcgccca tgacctacac ccggcgcgag accttcagcg acggcacgtg
     1381 cacctaccgg cagacgatca ccggagtcca gtcggaagcg acatcctcgt tctcgatcgg
     1441 gtcggcgacg tacaacggag acggattcct cgtccacgag gactacgccg tcaccgcgcg
     1501 ctgccgctag cgaccgcagc ggccgggcct ccggggctcg gccgcccggc tagctcgtcg
     1561 cgaagaacgc gatgagcgcg acgatcgcga gggcggcaag ggcggccgcc acgtagacgc
     1621 gccgtcgctc ctccccggtc atcccctcgc gggtcgagcc gctcgatggg cgcgacgccc
     1681 ggccgcgcca ctgcaggtag cgctcggtgt tgaggtcccg cgactcgcgc gaccggtagg
     1741 agcgccacgc cggccagccg atcaggtacg cgccggcggc cgccacgatg gcgatcacca
     1801 gccacaccag cggggacacg gcggtcagcc ctccttcacg gaggcgggca tcggctcggc
     1861 gccgtaggcg tcctggagga tcgcttcgac ctcgcgctgg tcgcgacgcc cgatgaagtg
     1921 ccacgccagg taggcgccgt tgagcagcgc ccagccgatg accaccggcg tgtagaacgt
     1981 cgaccacggc tcgccgaccc gctgaacggt ggcgttggcg agcagcaagc tgaccgcgaa
     2041 cagcgcggcg cccaccatcg tgaagtgcca cgtctcggcg gccagcatgg tcagcggccg
     2101 ccggagcgac gcggcaacga cgcgtggatc ccccggcgcg ccgaagtacg cctcctcgag
     2161 ccggtgtgcc accagcagct cgtcgccgag ggcgcggttg atgcggcgct cgagcgcggc
     2221 agcgtaggtg cgggcgaaga tcacgtagct gaacaggtgc gaggcatgga agcccacgta
     2281 caggatcacg agcgggagca gcagcgaacc ggcctgggtg ggcgcccaga aggagccgat
     2341 gaacaacagg ccgaccgcga ccgtcccgcc cgccacgaac cggaagaaga gctcgtagta
     2401 gcggtagagc atgctgcgca tccggccgag ctgcgcctcc aggatccgca gcccgtcggg
     2461 cgtcaacggc tcgctcacga acggtcctcg cccgcggcgg cggcatccac gaaccactcg
     2521 acgttgtcgc cgctgacgcg ccccgccgga tccgtggagg tcccggcccg gatgtcgccg
     2581 aaggcatcgg ccttgtcggc gccggtgacc acgaacagga cgtcgcggcc agcgttcagc
     2641 accgggaagg tgagcgtcat gcgccaggcg gacgggccgg gcgcccagtt ggcgaccacc
     2701 catcggtcca tcacggtgag cgcctgcgag cccgggaaga ggctggcgca gtggccgtcc
     2761 gggcccatgc cgagccagat gaggtcgaac gcgggcggct cctcgccacg ggccccgaag
     2821 gcgagccgca gctccgactc gtaggagagg gcggcggcat cgcggtccgg cgcctcggcc
     2881 ggcatgcggt ggatgcggtc ctggcgcaca ttgggcagct gcgagatgag catgccgtac
     2941 gcgacgccga agttcgactc ggggtggtcg ggcggcaccg cgcgctcgtc tccccagaag
     3001 aactccaccc ggctccagtc gacggcatcg cggcgctgtg gctccagcaa cagggggtaa
     3061 acctgcttcg gggtgctgcc gcccgagagc gcgacgcgaa agatcctgcg ctcctcgatg
     3121 gcggccacgg catcggcgac gaagcggtcc gcggtggcca tggcgacggc cgacggatcc
     3181 ggcaggacgg tgaagcgcgt catgcggcgg cctcgtgcgc ggacgagagg aggattgcgg
     3241 cggcgcgaag ggcgtcctcg tagacgcgat ccgcggtgtc gagcgtgagc tgcgcgctca
     3301 gcagctccgg ctcgcgcggg acctccatcc gcacccggcg caggagcgcg gtcatgccat
     3361 cggcgttggt tgccacggtt gcctcctcgg catcgcgatc gatgatgaac tcggcggcgc
     3421 ccgtctcgcc cagtgaccgc atgcgaacgg acagcagctc gccgggacgg accgtatcgg
     3481 tctcggccgg ctcgatgacg aggtcgacca tctcgtgtcg gccctcgagg cgcatgacgg
     3541 tccggccatc ggtgagcggc tgcagcgtct ggtgccgccg ccagcccagc cgcgtcgcca
     3601 tccaccccgc gtacagcagc gcctcggcca tcggagccga tacgccgggg gcgacctcct
     3661 cgtcctcgtc gtgccgctgc gatccgggcg gcgcggtcgc gtagcggatc cacaggcggc
     3721 tgaggttcgg caggtagcgg cggaagcgcg gcgcgtcgaa gaactgcgcc gtgagctcct
     3781 gccaccagct gagtcgctcc caggtcaggt cgccgacgcc gctgcggcgt cgcaggccgg
     3841 cgagacggcg cagcccgccg agcaggtcgc tgaagtcgga cgagtcgacc accacgcgat
     3901 cggccatctc gaccagctgc gcgaataccg ggtcggcgaa gggcgggtca cccggccacc
     3961 agacgtgggt tggcaggtcg tggatcagca gcggcgcgac gacgccggag aggtgcgccg
     4021 ccgcctcgcc acggacggtc aggatgacct cctcgtagca gatccggtcg ccgccgctgc
     4081 tcgtctcgtg gcagtgggtg ctgatccggg cgtcgagcgg cgcgtcgccg cgggaacgct
     4141 gcgggaccag gacgatggcc cgcgaggggt ggcggacccc cagccccatc agggtctgca
     4201 ccgcgcggtc ggcggcgtca gcgtcgacca ccgtgacgat gaggttcagg accgatgcgc
     4261 gggcgtgcgg caggcccttc tcgcgcacca gcgggtcccc gccgtcgtcc acgcccgccg
     4321 gcaggtcgaa cagcgtcgcc aggtgcgagg caatggcgcg caccgaggtg tcgcgctcct
     4381 cccacatcgg cgtgaccagc gtctcggcct cgccgaagcg catgagccgc cgatggaccg
     4441 cctgctcctc ggtggcggcg cgtcccccgt tggtcacggc ttgcgccacg accggccgtc
     4501 gcgctcgagc agctcgtcgg ccgccggcgg tccccacgtc ccggcgccgt agaagtgcag
     4561 cgggccgccc tcgcccgccg cccacgcgtt gaggatcggg tccaggatct cccaggcccg
     4621 ctcgacctcg tcgtcgcgcg tgaagaggct ggcgtcaccg accatcgcgt cgagcagcag
     4681 cgtctcgtag gcgtcgggcg agtcgacggc gaagctggcg ccatagcgga agtccatgtt
     4741 cacggtccgg atctgcaggc cctggccggg gaccttggcg ccgaagcgca gcaggatgcc
     4801 ctcgtcgggc tggacgcgga tggcgagcac gttggcgtcg atctgcggta cgcccgcgcg
     4861 ggcgaagagc gccagcggcg cgcgcttgaa ctgcaccgcg atctcggtca cgcggctggg
     4921 catggccttg ccggtgcgca ggtagaacgg cacgccggcc cagcgccacg agtcgatggc
     4981 gagcttcagc gcgacgaagg tctccgtctc ggaatcgggt gcgacctccg gctcgtcgcg
     5041 gtaggacggc accttctcac cctcgaccca gccggagacg tactggccgc ggaccgtgtt
     5101 ggccgccacc tcctcggggg tcatcggctt cacgccgcgc agcaccttca gcttctcgtc
     5161 gcgcaggtcc tcggccgcga actcgaccgg c
//
LOCUS       DASTYP010000047         8359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_986562, whole genome shotgun sequence.
ACCESSION   DASTYP010000047 DASTYP010000000
VERSION     DASTYP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986562"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFV59_02420"
     CDS             <1..588
                     /locus_tag="VFV59_02420"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEU5324714.1"
                     /translation="SAVQCAAWGLVRGSEVPASEQFQPAAAPAAPSGGSRWLALLARF
                     WWIPVAILVAVGGSLFAASRDDAGQITDGGDLGVADLRPGDCFDLKDASEELIDDVDA
                     KPCSETHEFEMFFVGSLTGDAYPAEETMTGYVIDNCIPAFDAYIGMDYQSSVYDIFHF
                     VPSEDGWNSGDHTVQCAVFHPTDDELTGSLKGAAQ"
     gene            652..2316
                     /locus_tag="VFV59_02425"
     CDS             652..2316
                     /locus_tag="VFV59_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEU5324715.1"
                     /translation="MRTDELAALAVALERRATTAPDRDDAERAERLRRHVAEYLLPRA
                     RHADAPLVVVLLGSTGSGKSSLFNALAGRALSPSGVLRPTTRRPIAVRHPDDAPDDLL
                     PGLAARGAVEMVADPEARRGLVVVDAPDFDSVELANRALAEELLEAADLVVFVTTATR
                     YADQVPWTILARARQRGVPLLAVLNRLPPDTPDAEAVTEDYRHLLERGGLTDAGAFGR
                     LEVVPVTEGMLDVQRDALDATAVAPIRAALDRLTADEVARRELARRSLDAALAGLPAA
                     IEEVAASVDVEVAAAEALLAAAHRAYRDRRQALADDLARGTFLRAEVLRQWQDFVGAG
                     QVARILAEGIGRLTAAVRALVRPGPAAPAAEVREAAFADLVAVTVQHADAASRRTATA
                     WLDEPHGAQALAEQPVLWGASADLGPRLDGALERWASDIADDIRALGEQRKGWAQVAS
                     IGVNAVGTSAILAVFVHTGGLTGAEVGITAATAVVNQKLLEAIFGEANVTAFTSRARN
                     ALAELLDEAFDRERDRYEAALGGLGQAAELADALRSTARSAAESAG"
     gene            2313..3899
                     /locus_tag="VFV59_02430"
     CDS             2313..3899
                     /locus_tag="VFV59_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU5324716.1"
                     /translation="MTLEKRLERLGAAADAAAGIGLDGPAHAAREVVETARRRVGFPG
                     DVYVLALAGGTGVGKSSILNALAGRTVSAVRAVRPTTDEPVAWVASDRVDELRPLLEW
                     LGVRHVASHADERLSGVAILDLPDVDSVRTEHRAAVDALLPRIDAVAWVVDPEKYDDE
                     RLHQYLRSLAPHAARLRFVLNKTDRLTEADRGAVASDLERRLAADAIMTPRIDAVSAA
                     TGDGMDGLRQALAGAADAKAVVIDKLSTDVAAAATALGRAAAVDRADPDRRLVPEERE
                     RAAIDEAVEGALAVVDPPGLARQVQAAVLGRARRTGGSLLGRVVGLLGWMTGQQRRRA
                     DPAGYLRDWRRRGSLGRVVNPVHRLLVEAAAALPPASRPAVLAALGAADLEAETARVL
                     DRVSRDAAGEVRIGGSWLWLPIGAVQLVVGAIFVFAVAWYVTLFVSGGSVPVATIEVP
                     VLGPVPLPLVLLAGSVIVSAVLGLILSLHAGWLGRRQGRRLASRVREAVEVAVREQGL
                     AGLHRVEAARAVIAASVLAE"
     gene            3964..4086
                     /locus_tag="VFV59_02435"
     CDS             3964..4086
                     /locus_tag="VFV59_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324717.1"
                     /translation="MFRREPNSRPSAVSDRELGVAALLLLLAVVLTTIIVPGLS"
     gene            4092..4253
                     /locus_tag="VFV59_02440"
     CDS             4092..4253
                     /locus_tag="VFV59_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324718.1"
                     /translation="MVTDFAAGTLIVLGVLIAVLGLFAAGDLVFVLAGLASVFAGGLL
                     GLLGRRRAA"
     gene            complement(4200..4508)
                     /locus_tag="VFV59_02445"
     CDS             complement(4200..4508)
                     /locus_tag="VFV59_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEU5324719.1"
                     /translation="MSDAIGAAAATWGVLMALSPVLQMRRMLERRSSADVSIAYLSVL
                     QIGFALWIGYGLTLANAALIVPNAVAFLIGVATIAIAVRYRSGGAAATEKAQQAAGED
                     "
     gene            4555..5772
                     /locus_tag="VFV59_02450"
     CDS             4555..5772
                     /locus_tag="VFV59_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5324720.1"
                     /translation="MLSRRRFLSGLLGAAAATVLTACAEAIRSLTPGASLSPTPSPTP
                     SLSPSPTATPSPTPTPTAAGLTLREKAAQLLLVGFRGLTTAEAPSVVADIAERGLGGV
                     VLFSRDQLTGGLRNVESPDQLTRLVADLRSGARVAPLIVAIDQEGGRVARLGPDHGFP
                     ATRSPAELGATGDPAQTRTAASQMARTEAGVGITLNLAPVVDLDINPENPIIGALDRS
                     FGGDPSTVIEHARAFIEAHHDAGVQCAIKHFPGHGSATGDTHLGVVDVTDVWSRTELE
                     PFADLTADGTTDAVLTAHIFNAALDPSYPATLSAATIGGVLREQLGWNGPVISDDLQM
                     RAIADAYGFEETVALALEAGIDLLLIANQLVYEPDIVTRAVDVIEGLVTSGRIGEDRL
                     DASIARTAVLRRP"
     gene            complement(5976..7646)
                     /locus_tag="VFV59_02455"
     CDS             complement(5976..7646)
                     /locus_tag="VFV59_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEU5324721.1"
                     /translation="MTLNPADDAPVHVDQRARMEILAAILLTMFLSALDQTVVGTALP
                     RIVTDLRGNELYTWVVTVYLLTATVSGPIYGKLSDQFGRRPMIMIGVSLFLIGSLLCG
                     LSQEMWQLILFRGLQGVGAGAIFPISLAVIGDLFSPRERGKYQGLFGAVFALSSLLGP
                     ALGGFLTDTISWHWVFLVNLPLGILALFVLWRLLPSVSHPERVRSIDYLGAAVFTAAL
                     VPFLLGLTNAQTGEWTDPQVGGLILLGLLLGGVFVWVESRAAEPILPLSLFRNRTVTA
                     SIVATFFITFGFFGAIIFIPRWFQFVAGSSATESGYQMLPLMVGVMGSSIVSGYLVAR
                     TGRYKWMTAGAMAIGAVGIGLMTNLRADTEITTLWIWMLVAGLGIGPSFAVFTIVVQS
                     AVAPTMLGAATSALTFFRQVGGSVGLAIAGTVFGTALTDQLPQRMSANGIPQPMIDAF
                     TSQGGGNPAGELTGVGTDLGAQILNSVPAQVRPAVEPFIGQIVDSIHQAFSLAIANTM
                     WLGLAGAVVSAAVVALLVPELTLRRAPSDSGGGDSGGERHTSPIPSFE"
     gene            complement(7643..8137)
                     /locus_tag="VFV59_02460"
     CDS             complement(7643..8137)
                     /locus_tag="VFV59_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5324722.1"
                     /translation="MPTTPSPSPSPTAITASSRIGEIIEAIEPLVAHQRRQWASRCHA
                     RGFSIVGAHLVALLETEGEVPMSRLAEALDVALPNATGIVGRLAERGIVERRHSEEDR
                     RVVLVRLTDAGHQLIGEMEQARRARLHRLISTMDDEQRERLMQAVQDLRTAARSLTEE
                     DELR"
BASE COUNT         1149 a         3005 c         3036 g         1169 t
ORIGIN      
        1 agcgcggtcc aatgtgcggc gtgggggctg gtccggggct cggaggtgcc ggcatcggag
       61 cagttccagc cggccgccgc cccggcggcg ccgagtggcg gctcgcgctg gctcgcgctc
      121 ctggcgcgct tctggtggat cccggtcgcc atcctggtcg cggtcggtgg ctcgctgttc
      181 gcggccagcc gtgacgacgc cgggcagatc accgatggcg gcgacctcgg cgtcgcggac
      241 ctgcgtcccg gcgactgctt cgacctgaag gacgccagcg aggagctcat cgacgacgtc
      301 gacgccaagc cgtgcagcga gacgcacgag ttcgagatgt tcttcgtcgg gtcgctgact
      361 ggcgacgcgt acccggccga agagaccatg accggctacg tgatcgacaa ctgcatcccg
      421 gccttcgacg cctacatcgg gatggactac cagagctcgg tgtacgacat cttccacttc
      481 gtccccagcg aggacgggtg gaactccggc gaccacaccg tccagtgcgc cgtcttccac
      541 ccgaccgatg acgagctgac cggctcgctg aagggcgccg cccagtaggt cgagcccgcg
      601 ccgccggcgg cgcgggaacg ggggcggccc ggatcggtca tccttgcgcg gatgcgcacc
      661 gatgagctgg ccgccctcgc cgtcgccctc gagcgtcggg ccaccacggc gccggaccgc
      721 gatgacgccg agcgtgcgga gcggctgcgg cgtcacgtcg cggagtacct gctgcctcgt
      781 gcccgccacg ccgacgcgcc gctcgtcgtg gtcctgctcg gctctaccgg cagcgggaag
      841 tccagcctct tcaacgccct cgcgggtcgg gcgctgagcc cgtcgggcgt gctgcgcccg
      901 acgacccggc gccccatcgc cgtccgtcac cccgatgacg cgccggacga cctcctgccc
      961 ggcctggcgg cgcgcggagc cgtcgagatg gtggccgacc cggaggcccg tcggggcctg
     1021 gtggttgtgg atgcgcccga cttcgacagc gtcgagctgg cgaaccgggc gctggccgaa
     1081 gagctgctcg aggcggccga cctggtcgtg ttcgtcacga ccgccacccg ctacgccgac
     1141 caggtgccgt ggaccatcct ggcgcgcgcc cgccagcgcg gcgtgccgct cctggccgtg
     1201 ctcaaccgtc tcccgcccga cacgccggat gccgaggcgg tcaccgagga ctaccgccac
     1261 ctgctcgaac gcggaggact gaccgatgcg ggcgccttcg ggcgcctgga ggtcgtgccg
     1321 gtgacggagg gcatgctgga cgtccagcgc gatgcgctcg acgcgacggc ggtcgccccg
     1381 atccgggcag cgctcgaccg gctgaccgcg gacgaggtgg cgcgtcgcga gctggcccgt
     1441 cggagcctcg atgcggcgct ggccggactc ccggccgcga tcgaggaggt cgcggcgtcg
     1501 gtcgacgtgg aggtggccgc ggccgaggcg ctgctcgctg ccgcacaccg ggcgtaccgc
     1561 gatcgccgcc aggcgctggc cgacgacctg gctcgtggca ccttcctgcg ggccgaggtg
     1621 ctgcggcagt ggcaggactt cgtcggcgcg gggcaggtgg cgcgaatcct ggccgagggc
     1681 atcggtcggc tcaccgcggc cgtccgcgcc ctcgttcgac ccggtccggc cgctcccgcc
     1741 gccgaggtcc gcgaggcggc cttcgctgac ctcgtggccg tcaccgtcca gcatgcggac
     1801 gccgcctcgc gacggacggc caccgcgtgg ctcgatgagc cacacggcgc ccaggcgttg
     1861 gcggagcagc cggtgctgtg gggcgcatcg gccgacctcg ggccgcgcct cgatggcgcg
     1921 ctcgaacggt gggcgtccga catcgccgac gacatccgcg ccctggggga gcagcgcaag
     1981 ggctgggcgc aggtggcgtc catcggcgtc aacgccgtgg gcacctccgc catcctggcc
     2041 gtcttcgtcc acactggcgg gctgaccggc gcggaggtcg gcatcaccgc cgcgacggcg
     2101 gtggtgaacc agaagctgct ggaggccatc ttcggcgagg ccaacgtcac cgccttcacg
     2161 tctcgcgcgc gcaacgcgct ggcggagctg ctcgacgagg cgttcgaccg ggagcgcgac
     2221 cgctacgagg ccgcgctcgg cgggctgggt caggcggcgg agctcgccga tgcgctccgt
     2281 tccaccgcac gcagcgccgc ggagtcggcc ggatgacgct ggagaagcgg ctcgagcgcc
     2341 tgggtgcagc ggcggacgcc gcggcgggca tcggccttga cggccctgcg catgccgccc
     2401 gcgaggtcgt ggaaaccgcc cgccgccgcg tcgggttccc cggcgacgtc tacgtgctcg
     2461 cgctggccgg ggggaccggc gtggggaagt cgtcgatcct caacgcgctc gccggccgga
     2521 ccgtgtcggc ggtccgtgcc gtgcgcccga cgaccgacga gcccgtcgcg tgggtcgcca
     2581 gcgaccgcgt ggacgagctg cgcccgctgc tggagtggct cggcgtgcgc cacgtcgcga
     2641 gccacgccga cgagcggctg tccggcgtgg ccattctcga cctgccggac gtggacagcg
     2701 tccggaccga gcatcgggcg gcggtggacg cgctgctgcc acggatcgac gcggtggcct
     2761 gggtcgtcga cccggagaag tacgacgacg agcggctcca ccagtacctg cgctcgctcg
     2821 ccccgcatgc ggcgcggctg cggttcgtgc tgaacaagac tgatcgcctg accgaggccg
     2881 accgtggtgc cgtggccagc gaccttgagc gtcgcctggc ggcggacgcg atcatgacgc
     2941 cacggatcga tgcggtctcc gcggcgaccg gagacgggat ggacggcctg cgccaggcgc
     3001 tggccggggc cgcggacgcg aaggccgtcg tgatcgacaa gctcagcacc gacgtggcgg
     3061 cggccgccac cgcgctcggg cgggcggccg ccgtcgatcg ggcggacccc gatcgacgcc
     3121 tcgtgcccga ggagcgtgag cgcgcagcga tcgacgaggc ggtcgagggc gccctggccg
     3181 tcgtcgatcc gccgggcctc gctcgccagg tccaggctgc cgtcctcggg cgggcgcgcc
     3241 gcaccggcgg ctcactcctc gggcgggtgg tcggcctgct gggatggatg accgggcagc
     3301 agcggcgacg cgccgatccg gccggatacc tgcgcgactg gcgacgacgc ggctcgctcg
     3361 ggcgcgtcgt gaaccccgtg caccggctgc tggtcgaggc cgcggccgcg ctgcctcccg
     3421 cgtcccgccc ggcggtgctc gcggccctcg gcgcggccga cctggaagcg gagacggccc
     3481 gcgtcctgga tcgcgtgtcg cgagacgcgg ccggcgaggt ccgcatcggc ggctcctggc
     3541 tgtggctgcc gatcggagcg gtccagctgg tcgtcggcgc catcttcgtc ttcgcggtcg
     3601 cctggtacgt gacgctgttc gtcagtgggg gctccgtgcc ggtggcgacc atcgaggtgc
     3661 cagtgctggg acccgtgccg ctgccgctcg tcctcctcgc cggctcggtg atcgtcagcg
     3721 cggtgctggg gctcatcctg tcgctccacg ccggatggct cggacggcga cagggacgcc
     3781 ggctcgcctc ccgcgtgcgc gaagcggtcg aggtggccgt gcgggagcag ggactcgccg
     3841 gactccaccg cgtggaagcc gcccgcgcgg tgatcgcggc ctcggtactg gccgaatgac
     3901 gtaggtggga cgcgaccctt gcggaattcc cgagcgagca agcaagccta cgctgacgtc
     3961 acgatgttcc gccgtgagcc caactcccga cccagcgccg tctccgaccg cgagctcggt
     4021 gtggctgccc tgctgctcct gctggccgtc gtgttgacca ccatcatcgt tcccggtctg
     4081 tcctgagccc gatggtgacc gatttcgccg ccggcacgct catcgtgctc ggcgtgctca
     4141 tcgccgtgct cgggctgttc gccgccggcg acctcgtctt cgtgctggcc ggcctcgcgt
     4201 cagtcttcgc cggcggcctg ctgggccttc tcggtcgccg ccgcgccgcc tgagcggtag
     4261 cggaccgcga tcgcgatcgt cgccacgccg atcaggaagg cgacggcgtt cggcacgatg
     4321 agcgccgcgt tcgccagcgt cagcccgtag ccgatccaca gcgcgaaccc gatctgcagc
     4381 acgctcaggt aggcgatcga gacgtccgcg gaggatcgcc gctcgagcat gcggcgcatc
     4441 tgcaggacgg gggagagggc catcagcacg ccccaggtgg cggccgcggc cccgatcgcg
     4501 tcggacatcg gggcacccat gctagcggcc cgtcgacggg tctaccatcg cccgatgctc
     4561 tcgcgtcgcc gattcctctc cggcctgctg ggggccgccg cggccaccgt cctcaccgcc
     4621 tgcgccgagg cgatccgatc gctcacgccc ggcgcctcgc tcagcccgac cccgtcaccc
     4681 acgcccagcc tctcgccgtc gcccacggcc acgccgtcac cgacgccgac gcccaccgcg
     4741 gcgggcctca cgttgcggga gaaggccgct cagctgctgc tcgtcggctt ccgtggcctc
     4801 accacggccg aggcgccgag cgtcgtcgcg gacatcgccg agcgtggact gggcggcgtc
     4861 gtgctgttct cgcgcgacca gctcaccggg ggccttcgca acgtcgagtc tccggaccag
     4921 ctcacgcgcc tcgtggccga cctgcgatcc ggcgcgcggg tggcaccgct catcgttgcg
     4981 atcgaccagg agggcggccg cgtcgcgcgg ctggggccgg accacggctt cccggcaacg
     5041 cgctcgccag cggagctggg tgccaccggc gatcccgcgc agacccgaac tgcggcatcg
     5101 cagatggcgc ggacggaggc cggggtgggc atcaccctca atctcgcgcc ggtcgtggac
     5161 ctcgacatca atccggaaaa cccgatcatc ggtgcgctgg atcggtcctt cggcggcgat
     5221 ccgagcacgg tgatcgagca cgcccgtgcc ttcatcgagg cgcaccacga cgccggcgtc
     5281 cagtgcgcga tcaagcactt ccctgggcac ggcagcgcca ccggcgacac gcacctcggc
     5341 gtggtggacg tgaccgacgt gtggagccgc accgagctgg agccgttcgc ggacctcacc
     5401 gccgacggca cgaccgatgc ggtcctcacc gcgcacatct tcaacgcggc gctcgacccg
     5461 agctatccgg cgacgctgtc ggccgcgacc atcggcggcg tcctgcgcga gcagctgggg
     5521 tggaacggcc ccgtcatcag cgacgacctt cagatgcgcg ccatcgccga tgcctacggg
     5581 ttcgaggaga ccgtcgcgct tgccctggag gccggcatcg atctgctgct catcgccaac
     5641 cagctggtgt acgagcccga catcgtcacc cgggcggtgg acgtcatcga gggcttggtg
     5701 acgtccgggc ggatcgggga ggaccgcctg gacgcgtcga tcgcgcgcac ggcggtcctg
     5761 cggcgaccgt aggtccggac cgggcgctcg cggcgacggg gccactcaca gccggggatg
     5821 gttactcgcg ggtcggtcgc agcccactcc gccacatcgg cgcttctcac gcttccaggg
     5881 cgtgaggcgg cagacccacg gctcctcgcc ccgaccttca cgcctcccag gcgtggatgg
     5941 ccggccgggg cgcagatcgg cgcgccccgc tcgccctact cgaagctcgg gatcgggctc
     6001 gtgtggcgct cgccaccgga gtcgccgccc ccgctgtcgc tcggtgcgcg tcgcagggtg
     6061 agctccggga cgaggagcgc caccacagcc gcggagacga ctgccccggc cagcccgagc
     6121 cacatcgtgt tggcgatggc aagcgagaag gcctggtgga tcgagtcgac aatctggccg
     6181 atgaacggct cgacggccgg acgtacctgg gccgggacgc tgttgaggat ctgcgccccg
     6241 agatcggtgc cgacgcccgt cagctcgccg gccgggttgc caccgccctg ggaggtgaag
     6301 gcgtcgatca tcggctgcgg gatgccgttg gcggacatcc gctgcggcag ctggtcggtc
     6361 agcgccgtcc cgaacaccgt gccggcgatg gccaggccca cggatccacc gacctggcgg
     6421 aagaacgtca gcgcactggt ggccgccccg agcatggtcg gcgccacggc gctctgcacc
     6481 acgatcgtga agacggcgaa cgacggaccg atcccgaggc cggcgaccag catccagatc
     6541 cacagcgtcg tgatctcggt gtcggcccgc aggttcgtca tgagcccgat gcccaccgcg
     6601 ccgatggcca tggccccggc cgtcatccac ttgtagcgcc cggtgcgcgc gaccaggtaa
     6661 cccgacacga tcgagctgcc catgacgccg accatgagcg gcagcatctg gtagccggac
     6721 tcggtcgcgc tggacccagc cacgaactgg aaccagcgcg ggatgaagat gatcgcgccg
     6781 aagaagccga aggtgatgaa gaaggtcgcc acgatcgacg ccgtcacggt ccggttgcgg
     6841 aagaggctga gcggcaggat cggctcggcg gcgcgcgact cgacccagac gaagacgccg
     6901 ccgagcagca ggcccagcag gatgaggccg ccgacctgcg gatcggtcca ctcgccggtc
     6961 tgcgcgttgg tcaggcccag caggaagggc acgagcgcgg cggtgaagac ggccgccccg
     7021 aggtagtcga tcgagcggac gcgctccggg tggctgaccg acggcagcag ccgccagagg
     7081 acgaagagcg ccaggatgcc gagcggcagg ttgaccagga agacccagtg ccagctgatc
     7141 gtatcggtca ggaagccgcc gagggcgggg ccgagcagcg acgacagcgc gaacacggcg
     7201 ccgaagagcc cttggtactt gccgcgctcg cgagggctga acaggtcgcc gatgacggcc
     7261 agcgagatcg ggaagatcgc gcccgcgccg acgccctgga ggccacggaa caggatgagc
     7321 tgccacatct cctgcgagag gccgcacagc agcgagccga tgaggaagag gctgacgccg
     7381 atcatgatca tcggccgccg cccgaactgg tcggagagct tgccgtagat cggtccgctg
     7441 acggtcgccg tcagcaggta gacggtgacg acccaggtgt acagctcgtt gccgcgcagg
     7501 tcggtcacga tccgcgggag ggccgtgccc accaccgtct ggtcgagggc cgacaggaac
     7561 atcgtcagga ggatggcggc caggatctcc atcctggccc gctggtcgac gtggaccggc
     7621 gcgtcgtcgg ccggattcag ggtcatcgga gttcgtcctc ttcggtgagg gagcgggcgg
     7681 cggttctcag gtcctggacc gcctgcatca atcgctcccg ctgttcgtca tccatcgtgc
     7741 tgatgagccg atgcaggcgc gcgcgccgcg cctgctccat ctcgccgatc agctggtgcc
     7801 cggcatcggt caaccgcacg aggacgaccc gtcgatcctc ctcgctgtgc cgccgctcga
     7861 cgatgccccg ctccgccagc cgccccacga tgcccgtggc gttgggcagc gccacgtcga
     7921 gcgcctcggc caggcggctc atcggcacct cgccctccgt ctcgagtagc gcgaccaggt
     7981 gcgcgccgac gatcgagaaa ccacgcgcat gacagcgcga cgcccactgg cggcgctgat
     8041 gcgccaccag cggttcgatg gcctcgatga tctcgccgat ccgcgaggag gcggttatcg
     8101 ccgtcgggct cgggctcggg ctcggagtgg tgggcatcgg gcaattatgg ttgtcataac
     8161 cattgcgtca aatcgaagta agtctgtgac ttcaggcccg tccggtccgc acgcagtgcc
     8221 gtgtcgtgga caggctgtgg atagctgcgt gctggcggcg aaaagtcggg gataagtggg
     8281 attgcgtctc gcctcgggcg gcgggcatac tgtggtcgtc ccgaaggggc aagcgcaccc
     8341 ggatcacacg atttcgatc
//
LOCUS       DASTYP010000048         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_659057, whole genome shotgun sequence.
ACCESSION   DASTYP010000048 DASTYP010000000
VERSION     DASTYP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659057"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1072
                     /gene="tsaD"
                     /locus_tag="VFV59_02465"
     CDS             26..1072
                     /gene="tsaD"
                     /locus_tag="VFV59_02465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5324723.1"
                     /translation="MSAPRILAIESSCDETGVAVVVGGRRIEANQVATQVALHAATGG
                     IVPEVAARHQLRWIVPTLEAAMAEAGVDWPDIDAVAVTYGPGLIGSLLVGVNVAKALA
                     VAHDLPLVGVNHIEGHIYANWLTDVEADAPLPAEPEFPLLNLVVSGGHTQLVLMEDHG
                     RYRLLGQTVDDAAGEAFDKVGRLLGLPYPGGPAISAAAVGSTPATRFPRARTEGPYDF
                     SFSGLKTAVLREVQGYRDRNEPIPVDGLAAAFEEAVVDALATKTVAAARDHGVAEVAL
                     GGGVAANRALRATLQQRLAAEGLPLRVPPPAWCTDNGAMIGAAAGFRYAAGDRADAAL
                     EAVPNLSLPWLERP"
     gene            1069..1938
                     /gene="rsmA"
                     /locus_tag="VFV59_02470"
     CDS             1069..1938
                     /gene="rsmA"
                     /locus_tag="VFV59_02470"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU5324724.1"
                     /translation="MTDPLAPERLAPPTPGQLRRLMGVERLRPRKSLSQNFLTDPEAL
                     DAIVAAAELEPGDRVVEVGPGLGVLTRRLLAAGASVLAVELDPRLAAYLRRELGGVDG
                     FELIEADALSLHPREMFPGEPFKVVANIPYHITSPLLHAFLEGERPPELTVLLVQLEV
                     AERVASPPGGMSYLSVFAQNVAEAEVVARVPAAAFEPEPAVDSAVLRLRRRAEPYVAP
                     GDGREPFYRVVQAGFRERRKQVHNGLTRELPVDRDAVEAALAACDVARDRRPQTLSVP
                     EWACLADRLAPLL"
     gene            1938..2765
                     /locus_tag="VFV59_02475"
     CDS             1938..2765
                     /locus_tag="VFV59_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU5324725.1"
                     /translation="MDPLRLEAPAKLNLSLRVVGRRDDGYHELESELVLLDLADRLLV
                     LPGCSGLRVEGRGAADLPLDERNLAWRGLVAGLGGPPDLVCLTVEKRIPSAAGLGGGS
                     SDAAAAWRLGRTLAGEAERATPDDLRELARIGADVPFFAAGVPVARVGGIGEVVEPTP
                     AVDASVLLVHPPFALSTADVFTELRREEWGTHDNDLLPPARRLRPELDDLFAAVHRAG
                     GTPRLTGSGPTIFCLDDDPERLGVVATRLEATGLETTLTRTRAAAPQIERIHEEELE"
     gene            2762..3154
                     /locus_tag="VFV59_02480"
     CDS             2762..3154
                     /locus_tag="VFV59_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5324726.1"
                     /translation="MTSREAIHTDAAPAAAGPYSQAIRANGFVYTAGQLGLDPATGEL
                     AGADVAAQAERALQSLAAILDAAGSGMDRLVKVTVFLADIGDWPAVNEVYGRLVPEPY
                     PARSAFAVRDLPKGALVEIEAVAVTGAG"
     gene            3189..>3346
                     /locus_tag="VFV59_02485"
     CDS             3189..>3346
                     /locus_tag="VFV59_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU5324727.1"
                     /translation="MSAQPTTAAAIVLAAGQGTRMRSRIAKVLHPLAGRTLLDHVLAA
                     LAEAGVPAP"
BASE COUNT          412 a         1245 c         1246 g          443 t
ORIGIN      
        1 ccgcgcagcg ccagctgatc ggcacgtgag cgctccccgc atcctcgcca tcgaatcctc
       61 gtgcgacgag accggcgtcg cggtggtcgt cggtgggcgt cgcatcgagg cgaaccaggt
      121 ggcgacgcag gtcgcgctgc atgccgcgac cggcgggatc gtgcccgagg tggcggcgcg
      181 ccaccagctg cgctggatcg tgccgaccct cgaggccgcc atggccgagg cgggggttga
      241 ttggcccgac atcgacgcgg tggcggtgac ctacgggccg gggctcatcg gctcgctgct
      301 ggtcggggtc aacgtcgcca aggccctggc ggtcgcccac gaccttccgc tggtcggcgt
      361 caaccacatc gaggggcaca tctacgccaa ctggctgacc gatgtcgagg ccgatgcgcc
      421 cctccccgcc gagcccgagt tccccctgct caacctcgtc gtcagcggcg ggcacaccca
      481 gttggtgctg atggaggacc acgggcgcta tcggctcctg gggcagacgg tggacgacgc
      541 cgccggggag gcgttcgaca aggtcgggcg gctgctcggc ctgccgtacc ccggaggccc
      601 cgccatcagt gccgccgcgg tcggctcgac gccggccacc cgcttcccgc gggcgcggac
      661 ggaggggccg tacgacttca gcttctccgg gctcaagacg gcagtgctgc gcgaggtgca
      721 gggctaccgc gaccggaacg agccgatccc ggtcgacggc ctggccgcgg ccttcgagga
      781 ggccgtcgtc gacgcgctgg ccacgaagac ggtggccgcc gcccgggacc acggcgtggc
      841 cgaggtcgcc ctgggcgggg gcgtggccgc caaccgcgcg ctgcgcgcca cgctgcagca
      901 gcgcctggcc gccgaagggc tgccgctgcg cgtcccgccg ccagcctggt gcaccgacaa
      961 cggcgcgatg atcggggccg cggcgggctt ccggtatgcc gccggcgacc gcgcggatgc
     1021 cgcgctcgag gcggtgccga acctctccct gccgtggctg gagcgtccgt gaccgatccg
     1081 cttgcgcccg agcgcctggc gccgccgacg ccgggccagt tgcggcggct gatgggcgtc
     1141 gagcggctgc ggccgcgcaa gagcctgagc cagaacttcc tgaccgatcc ggaggccctc
     1201 gacgccatcg tggcggcggc cgagctggag cccggcgacc gcgtcgtcga ggtcggcccg
     1261 gggctcgggg tgctcacccg ccggctgctg gcggccggcg catcggtcct ggcggtggag
     1321 ctggatccgc ggctggccgc gtacctgcgt cgcgagctgg gtggcgtcga tggcttcgag
     1381 ctgatcgagg cggatgcgct gtcgctccac ccacgcgaga tgttcccggg cgagccgttc
     1441 aaggtggtgg cgaacatccc gtaccacatc accagcccgc tgctgcacgc cttcctggag
     1501 ggcgagcggc caccggagct gaccgtcctg ctggtacagc tggaggtcgc cgagcgcgtc
     1561 gcctcgcccc ccgggggcat gagctacctc agcgtgttcg cccagaacgt cgccgaagcc
     1621 gaggtcgtgg cccgcgtccc tgcggcggcg ttcgagcccg agccggcggt ggactccgcc
     1681 gtgctccgcc tccgacgacg cgcggagccg tacgtggcgc cgggggacgg ccgcgagccg
     1741 ttctatcgcg tcgtgcaggc cggcttccgc gagcggcgga agcaggtgca caatggcctc
     1801 acccgcgagc tgccggtcga ccgcgacgcg gtggaggccg ccctcgccgc ctgtgacgtt
     1861 gcccgcgacc gccgaccgca gaccctcagc gtgcccgagt gggcgtgcct cgccgaccgg
     1921 ctggcgccac tcctgtgatg gatccgctgc gcctggaggc gccggccaag ctcaacctgt
     1981 cgctgcgcgt cgtcggacgc cgggacgacg gctaccacga gctcgagagc gagctcgtcc
     2041 tgctcgacct ggccgatcga ctgctggtcc tcccgggctg cagcggcctg cgggtcgagg
     2101 gccgcggagc cgccgatctg ccgctcgacg agcgcaacct ggcgtggcgc ggcctggtcg
     2161 ccggcctcgg tggtcccccc gacctcgtct gcctgaccgt cgagaagcgg atcccgtcgg
     2221 cggccgggct gggcggcggc tcctcggacg ccgcggccgc gtggcggctg ggacgaacgc
     2281 tggcgggcga ggcggaacgc gcgacgccgg acgacctgcg tgagctggcc cggatcgggg
     2341 ccgacgtgcc gttcttcgcg gccggtgtgc cggtggcgcg cgtcggcggc atcggcgagg
     2401 tcgtcgagcc cacgccggcc gtggacgcat cggtcctgct cgtccacccg ccgttcgcgc
     2461 tgtcgaccgc tgacgtcttc accgagttgc gccgcgagga gtggggcacc cacgacaacg
     2521 acctgctccc gccggcccgc cggctgcgac cggagctgga cgacctgttc gccgccgtcc
     2581 atcgcgcggg agggacaccc aggctgacgg gcagcggccc gaccatcttc tgcctggacg
     2641 acgatcccga gcgcctgggc gtcgtcgcca cgcggctcga ggcgacgggc ctggagacga
     2701 cgctcacccg cacgcgcgcc gcggcgccgc agatcgaacg catccacgag gaggagctcg
     2761 aatgacatcc cgcgaggcca tccataccga tgccgccccg gcggcggcag gtccctacag
     2821 ccaggcgatc cgcgccaacg gcttcgtgta caccgccggg cagctggggc tggatccggc
     2881 caccggcgag ctcgccggcg cggacgtcgc cgcgcaggcc gagcgcgcgc tgcagagcct
     2941 ggccgccatc ctggacgccg cgggcagcgg gatggaccgg ctggtgaagg tgacggtctt
     3001 cctggccgac atcggcgact ggccggcggt caacgaggtg tatggacgcc tcgtcccgga
     3061 gccctacccg gcgcgcagcg cgttcgccgt gcgcgacctg cccaagggcg cgttggtcga
     3121 gatcgaggcg gtcgcggtga ccggcgccgg ttgacgcttc gaatcggcca cctctaccat
     3181 cgcgcaacgt gtctgcacag ccgacgaccg ccgcggccat cgtcctggcc gccggccagg
     3241 gcactcgcat gcgctcccgc atcgccaagg tgctgcaccc cctcgccgga cgaacgctgc
     3301 tcgaccatgt cctggcggcg ctggcggagg ccggcgtccc ggcgcc
//
LOCUS       DASTYP010000049         6841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1241886, whole genome shotgun sequence.
ACCESSION   DASTYP010000049 DASTYP010000000
VERSION     DASTYP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241886"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="VFV59_02490"
     CDS             <1..2073
                     /locus_tag="VFV59_02490"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEU5324728.1"
                     /translation="GRLNVLAHVVGVSYQAILAEFEAGRAGAEAATGPKGGSDDVKYH
                     LGAEGGYLTAEDEVVHVVLSPNPSHLEAVNPVIEGRARAEQTDRSTPVATVDPTSTLP
                     VLIHGDAAFAAQGVVAETFNLAKLDGYATGGTIHLIANNQVGFTTGPHEGRSTDYSSD
                     LAKGFDAPIIHVNADDPEACLAAARLAMMYRERFGSDVVIDLVGYRRFGHNEGDEPAY
                     SQPSMYKAIADHATVRERYQAELIEQGVISAQEADAKVKAAQRELAEHQAALRKGLED
                     GEGELDRGSEAIPPEQLSDPETAVAVDRLRELNRALGAVPDGFTVHPKLTKQLERRAT
                     ALDVPDEARVDWAHAELLAFASLLAEGTPIRLTGQDTVRGTFSQRHAGLWDARTGQRH
                     VPLQHLPGSRASFEVHNSPLSEYACLGFEYGYAVTARDALVLWEAQYGDFVNGAEIII
                     DQFLIAGLAKWRQTSRLTLLLPHGYEGSGPEHSSARLERFLALGAEENIRVVYPTTPA
                     QYFHLLRRQALHHDARPLVVMTPKSLLRLPAAASRGADLAEGRFRPVIDDPRFAADAK
                     AAASVKRLILCSGKVYYDLIGSDLFEKARDVAVVRVERLYPFPTDELQVVIDRYPKIE
                     SFAWVQEEPRNMGARKFVLPKIRHLVPYKIPLGDISRPERSRPAEGYPAAHTIEQARI
                     VREALTGR"
     gene            2306..3364
                     /gene="thrC"
                     /locus_tag="VFV59_02495"
     CDS             2306..3364
                     /gene="thrC"
                     /locus_tag="VFV59_02495"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5324729.1"
                     /translation="MTERQLPLLERYRDLLARPDESFPITLGEGGTPLIHARRLGAEI
                     GVDRLYLKFEGTNPTGSFKDRGMVLAVNRAVAAGAKAVVCASTGNTSASAAAYAAAAG
                     LPCHVILPAGKVARGKLAQALAAGARLVMVDGNFDAALEAVRRLGDEGVAVVVNSINP
                     DRLEGQQTAAWEVADALGAAPDVLALPVGNAGNITAYWRGFNRYADEGRATTRPRMLG
                     FQAEGAAPIVRGEPVTDPETVATAIRIGNPASWAGAVAARDESGGLIATVSDDEILEA
                     QRLIVQLEGVFCEPASAASVAGIRRLAAEGGLGREEAVVCVLTGHGLKDPDAVQAEEG
                     TLTPIPPDLGSVRAAIGV"
     gene            complement(3366..3770)
                     /locus_tag="VFV59_02500"
     CDS             complement(3366..3770)
                     /locus_tag="VFV59_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5324730.1"
                     /translation="MPLESRLPLSTTPRVVIVDADRRVQSSLADLLGVTHEVSVVGRA
                     GDVRSALQVIESEHPDAVLVDPRLPDVEAGIALISGIARAWPDMRIVLTGWNDTGGHS
                     LTPVAATRYVSKNASPEEFVQAVVQACCPDGC"
     gene            complement(3871..>6841)
                     /locus_tag="VFV59_02505"
     CDS             complement(3871..>6841)
                     /locus_tag="VFV59_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5324731.1"
                     /translation="DGGRIELVAEGGGLHWAYGHSAIDLPIERDEAIDPVQADEGVSG
                     RAVIERRVVRTGDYLADTSFTHSPAPDRYIRQHGIRSVMSAPLIGDEGAIGSLTVHSQ
                     AREAFDEANAELLEVLASQAAIAVTNARLYEQLRERIDAMEALAAISAEIVSLRDPGA
                     VLQRTVEEAMRLLDADTAIINPLETSEELLGWPIAYAPADGPQDDIPVRLGAGVSGRA
                     MAERRVIRTGDYLHDPAFDHGPDLDEYIGRRGMRSVMSAPLVSSSGDLGALTVQAHRL
                     HAFKDNDEQLIRVLADQAAIAITNARLYDELGNESTALARQIDSQRRLLQINQRLLST
                     LEPASVLELIADGLKSVVWYDNLGLYRVDEDEEVLRPVLARDRNAEAVLGFPIPKGQG
                     LTWWSVEHREPVLLNDALHDPRVIQIPGTPPEEEAMIIVPLISGDEVIGAMNIARSGG
                     HDIAFTDADFEMVQLFAGQAAIAVTNARLWEELRERSDAQRALAEIAAQIAALHDPVT
                     VIQRAVADAARLLRADRAQINLAAEDATHLERPIAAAPVPPSPDDVVVPLGSGIAGMA
                     AAERRVRWTGDYLADATFPHDEGDRRIEAQGIRSMMSAPLLGSDRLLGTITIQTTARN
                     AFNRQDAELLKLLADQAAIALTNARLYAELEESERRYRHLVDNSPDIVWSVDTDGRFT
                     FFSDSLEARTGWKSEQLIGQPFTVLAAQGSLQAAATAWEELRQHPGREQRVRLDLPLP
                     DGRLAQTEVAMTGTIVDGRFAGAHGSVRDISERERLEGDLRRQAAELAASQERAHLAR
                     ELHDSVTQALFSMGLTLRTLELLLSTDPAAAQPKLAELRELQKDALAEMRTLIFELRP
                     SSLESDGLIQALRTHATAVQRRTGLTIVVDAEPIERLSLNAEEALYRIGQEALHNIVK
                     HANASNATIRIVREGNRVRLSVSDDGAGFAPDEVPRGHLGLIGMRQRVELVGGDLRVE
                     SRPGRGTTIEASVPTGD"
BASE COUNT          959 a         2445 c         2463 g          974 t
ORIGIN      
        1 ggccggctca acgtgctggc ccacgtggtc ggcgtcagct accaggcgat cctggccgag
       61 ttcgaggccg gacgcgccgg ggcggaggcg gccacggggc cgaagggcgg ctccgacgac
      121 gtgaagtacc acctgggcgc ggagggcggc tacctgaccg ccgaggacga ggtcgtccac
      181 gtggtgctct cgccgaaccc gagccacctc gaggcggtga accccgtgat cgagggtcgt
      241 gcacgggcgg agcagacgga ccgatccacc ccggtcgcca ccgtcgaccc gacgtccacg
      301 ctgcccgtcc tgatccacgg cgacgccgcc ttcgcggccc agggcgtcgt ggccgagacc
      361 ttcaacctgg ccaagctcga cggctacgcc acgggcggca ccatccacct catcgccaac
      421 aaccaggtcg gcttcaccac cggcccgcac gagggccgat ccaccgacta ctcgtcggac
      481 ctggccaagg gcttcgacgc gccgatcatc cacgtcaacg ccgatgatcc cgaggcctgc
      541 cttgcggcgg cccggctggc gatgatgtac cgggagcgat tcggcagcga cgtggtgatc
      601 gacctggtcg gctaccggcg gttcggccac aacgagggcg acgagccggc gtactcgcag
      661 ccgtccatgt acaaggccat cgcggaccac gcgacggtgc gcgagcgcta ccaggccgag
      721 ctgatcgagc agggcgtgat ctctgcgcag gaggccgatg cgaaggtcaa ggccgcccag
      781 cgcgagctcg ccgagcacca ggcggcgctg cgcaagggcc tcgaggacgg cgagggcgag
      841 ctcgaccgcg gatccgaggc gattccgccg gagcagctct ccgatcccga gaccgccgtc
      901 gccgtcgacc gcctgcgcga gctgaaccgg gcgttgggtg cggtgccgga cggcttcacc
      961 gttcatccca agctgaccaa gcagctcgag cgtcgggcca ccgccctcga cgtacccgac
     1021 gaagcgcgcg tggactgggc gcacgccgag ctgctggcct tcgcgtcgct gctggccgag
     1081 gggacgccca tccgcctcac cggccaggac accgtgcgcg gcaccttcag ccagcgccac
     1141 gccgggctgt gggacgcgcg caccgggcag cgccacgtgc cgctccagca ccttcccggc
     1201 tcgcgggcca gcttcgaggt gcacaactcg ccgctgtcgg agtacgcgtg cctcggcttc
     1261 gagtacggct acgcggtgac cgcacgcgac gcgctcgtcc tgtgggaggc gcagtacggc
     1321 gacttcgtca acggggcgga gatcatcatc gaccagttcc tgatcgccgg actggccaag
     1381 tggcgccaga cctcgcgcct caccctgctg ctgccgcatg gctacgaggg ttccgggcct
     1441 gagcattcct ccgcccggct ggagcggttc ctcgcgctcg gcgccgagga gaacatccgc
     1501 gtcgtctacc ccaccacgcc ggcgcagtac ttccaccttc tgcggcgcca ggcgctccac
     1561 cacgacgcgc gcccgctggt ggtcatgacg cccaagtcac tgctgcgcct cccggctgcc
     1621 gcctcgcgcg gcgccgatct ggccgagggt cgcttccgcc cggtcatcga cgacccacgc
     1681 ttcgcggccg atgcgaaggc cgcggcatcg gtcaagcgcc tgatcctgtg cagcggcaag
     1741 gtctactacg acctcatcgg ctccgacctg ttcgagaagg cccgcgacgt ggccgtcgtc
     1801 cgggtcgagc gcctctaccc cttccccacg gacgagctgc aggtcgtcat cgaccgatac
     1861 ccgaagatcg agtccttcgc gtgggtccag gaggagccgc gcaacatggg tgcgcgcaag
     1921 ttcgtgctcc ccaagatccg ccacctcgtg ccgtacaaga tcccgctggg cgacatctcg
     1981 cggcccgagc ggtcgcgacc agcggagggg taccccgcgg cacacaccat cgagcaggcg
     2041 aggatcgtcc gggaggcgct caccgggcgg taggcggcgc gcttcggtgt cgcgatgtat
     2101 cgagcccgat gcaccggacg cacggtccgc gcgctgccgc cacgatgcat cgcgaggcgc
     2161 accgcacgac ggatgcgcgt tggcttcact ccggcattgg gggccgcgat tgatcgcgga
     2221 atcacggcac ggcggccgcc cggcgttcag ttctcgcgat agatcgcgac acgccggcac
     2281 cgaaccggct ccctatactc cgcccgtgac cgaacgccag ctgccgctgc tggagcgcta
     2341 tcgcgacctc ctggcccgcc ccgatgagtc cttccccatc accctcggcg agggtggcac
     2401 gccgctgatc cacgcccgac ggctgggcgc ggagatcggg gtggatcgcc tctacctcaa
     2461 gttcgagggg accaacccca ccggcagctt caaggaccgc ggcatggtgc tcgccgtgaa
     2521 ccgggccgtc gccgccgggg cgaaagcggt cgtctgcgcc tccaccggca acacgtcggc
     2581 ctccgccgcc gcctacgctg ccgccgcggg actgccgtgc cacgtgatcc tgccggccgg
     2641 caaggtcgcc cgaggaaagc tggcccaggc gctggccgcc ggggcgcggc tggtcatggt
     2701 cgacggcaac ttcgacgcag cgctcgaggc ggtgcggcgg ctgggtgacg agggggtcgc
     2761 cgtcgtggtg aactcgatca accccgaccg gctcgagggc cagcagaccg ccgcctggga
     2821 ggtggccgac gcactgggcg cggcgcccga cgtgctggcc ctgcccgtcg gcaacgccgg
     2881 gaacatcacc gcctactggc gcgggttcaa ccgatacgcc gatgagggcc gggccaccac
     2941 gcgcccccgc atgctggggt tccaggccga aggcgccgca ccgatcgtgc ggggcgagcc
     3001 cgtcaccgat ccggagacgg tggcgaccgc catccgcatc ggcaatcccg cgtcgtgggc
     3061 cggtgccgtc gcggcgcgtg acgagtccgg cgggctgatc gccacggtga gcgacgacga
     3121 gatcctggag gcccagcggc tgatcgtgca gctggagggc gtcttctgcg agcccgcatc
     3181 ggctgcgagc gtggccggga ttcggcggct ggccgcggag ggcggcctgg ggagggagga
     3241 ggcggtcgta tgcgtgctga ccgggcacgg cctgaaggac ccggatgcgg tgcaggcgga
     3301 ggagggaacc ctgacaccga tcccgcccga cctcggcagc gtgagggccg ccatcggggt
     3361 gtgattcagc agccgtcggg acagcaggcc tgcaccacgg cctggacgaa ctcctcgggc
     3421 gacgcgttct tgctgacgta gcgcgtcgct gccaccgggg tcagggagtg cccgccggtg
     3481 tcgttccagc cggtaaggac gatccgcatg tccggccagg cccgggcgat cccgctgatc
     3541 agcgcgatcc cggcctcgac atcgggcagc cgagggtcga ccagcacggc gtcgggatgc
     3601 tccgactcga tgacctggag cgcgctgcgg acgtccccgg cgcgcccgac caccgatacc
     3661 tcgtgggtca cgcccagcag atcggcgagg ctggactgca cccgccgatc ggcgtccacg
     3721 atcaccactc gcggcgtcgt ggacagcgga agacgcgact cgagtggcat ggaatcgagc
     3781 gtacgcttcg ctcggcagag cgcgaccggc gggatggccg agctgcgtct acgtcattcg
     3841 gccgagacgg ccgacacgtg agccgggcgg tcagtcgccg gtcgggacgc tcgcctcgat
     3901 ggtcgtcccg cgacccgggc ggctctcgac ccgcaggtcg ccgccgacca gctcgacgcg
     3961 ctgtcgcatt ccgatcagcc ccaggtgacc gcgcggtacc tcgtccggcg caaacccggc
     4021 gccgtcgtcg ctgaccgaga ggcggaccct gttcccctcg cgcacgatcc ggatggtggc
     4081 gttcgaggcg ttggcgtgct tgacgatgtt gtggagcgcc tcctgtccga tgcgatacag
     4141 cgcctcctcg gcgttgagcg acaggcgctc gatcggctct gcgtcgacca cgatggtcag
     4201 gccggtgcgg cgctggacgg ccgtggcgtg cgtgcgcagc gcctggatga gcccgtcgga
     4261 ctccagcgag gacgggcgca gctcgaagat gagcgtccgc atctcggcca gcgcgtcctt
     4321 ctggagctct cgcagctcgg cgagcttggg ctgcgcagcg gccggatcgg tcgacagcag
     4381 cagctcgagc gtgcggagcg tgaggcccat gctgaacagg gcctgggtga ccgagtcgtg
     4441 cagctcgcgg gcgaggtgcg cacgctcctg gctggcggcc agctcggcgg cctggcgtcg
     4501 caggtcgccc tccagccgct cccgttcgct gatgtcgcgg acggagccat gcgcgccggc
     4561 gaaccggccg tcgacgatgg tgcccgtcat ggcgacctcg gtctgcgcca ggcggccgtc
     4621 gggcagcggc aggtccaacc ggacgcgctg ctcgcgcccg ggatgctgcc gcagctcctc
     4681 ccacgcggtc gccgccgcct ggaggctccc ctgcgcggcg agcaccgtga acggctgacc
     4741 gatgagctgc tccgacttcc agccggtgcg ggcctccagc gagtcgctga agaaggtgaa
     4801 gcggccatcg gtgtcgacgc tccacacgat gtccggcgag ttgtcgacca ggtgccggta
     4861 ccggcgctcc gactcctcga gctcggcgta gaggcgcgcg ttggtcaggg cgatggccgc
     4921 ctggtcggcc agcagcttga gcagctcggc gtcctggcgg ttgaacgcgt tgcgcgccgt
     4981 ggtctggatg gtgatggtgc ccagcagccg atccgatccc agcagcggcg ccgacatcat
     5041 cgagcggatg ccctgcgcct cgatgcgccg atccccctcg tcgtgcggga aggtcgcgtc
     5101 ggccaggtag tcgcccgtcc accgcacgcg gcgctccgcg gccgccatgc cggcgatccc
     5161 cgaaccgagc ggcacgacca cgtcgtcggg ggagggcggc accggcgccg ccgcgatcgg
     5221 ccgctcgagg tgcgtcgcgt cctcggcggc gaggttgatc tgggcgcggt cggcgcgcag
     5281 caggcgggcg gcatcggcca ccgcgcgctg gatgaccgtc accgggtcgt gcagcgccgc
     5341 gatctgggcg gcgatctcgg ccagcgcccg ctgcgcatcg gagcgctcgc gcagctcctc
     5401 ccagagtcgc gcgttcgtca cggcgatggc ggcctggccc gcgaagagct gcaccatctc
     5461 gaagtccgcg tcggtgaagg cgatgtcgtg cccgcccgag cgggcgatgt tcatggcgcc
     5521 gatgacctcg tcgccgctga tcagcgggac gatgatcatc gcctcctcct ccggcggggt
     5581 cccggggatc tggatcacgc gcgggtcgtg gagggcgtcg ttgaggagca ccggctcgcg
     5641 gtgctcgacg ctccaccagg tcaggccctg gcccttcggg atcgggaagc ccagcaccgc
     5701 ctccgcgttg cggtcgcggg caagcacggg ccgcaggacc tcctcgtcct cgtccacgcg
     5761 gtagaggccc aggttgtcgt accagacgac cgacttgagc ccatcggcga tgagctccag
     5821 cacggacgcc ggctcgaggg tcgacagcag gcgctggttg atctgcagca gccggcgttg
     5881 cgagtcgatc tggcgggcca gggcggtcga ctcgttgccc agctcgtcgt acaggcgggc
     5941 gttggtgatg gcgatggcgg cctggtcggc caggacccgg atcagctgct cgtcgttgtc
     6001 cttgaaggca tgcagccgat gcgcctgcac ggtcagggct cccaggtcgc ccgagctcga
     6061 caccagcggg gcgctcatca ccgaccgcat gccgcggcgc ccgatgtact cgtcgaggtc
     6121 ggggccgtgg tcgaaggcgg ggtcgtggag gtagtcgccg gtgcggatca cgcgccgctc
     6181 ggccatggcg cggccgctga cgccggcacc caggcgcacc gggatgtcgt cctgcggccc
     6241 gtcggcgggc gcgtaggcga tcggccagcc cagcagctcc tcgctggtct ccagcgggtt
     6301 gatgatcgcg gtgtcggcgt cgagcagacg catggcctcc tcgaccgtgc gctggagcac
     6361 ggctccgggg tccctgagcg agacgatctc ggcgctgatg gcggcgagcg cctccatggc
     6421 gtcgatgcgc tcgcgcagct gctcgtacag gcgggcgttg gtcacggcga tggccgcctg
     6481 gctggccagc acctccagca gctccgcgtt ggcctcgtcg aacgcctcgc gggcctggga
     6541 gtggacggtg agcgagccga tggcaccctc atcgccgatc agtggcgcgc tcatgaccga
     6601 gcgaatgccg tgctggcgga tgtatcggtc cggcgccggc gagtgggtga agctcgtgtc
     6661 ggcaaggtag tcgccggtgc gcaccacgcg tcgctcgatc accgcgcgac cggagacgcc
     6721 ctcgtcggcc tggaccgggt cgatcgcttc gtcgcgctcg atcggcaggt cgatggcgga
     6781 gtggccgtac gcccagtgca gcccgccgcc ctcggccacc agctcgatcc gtccaccgtc
     6841 g
//
LOCUS       DASTYP010000050        20554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1643138, whole genome shotgun sequence.
ACCESSION   DASTYP010000050 DASTYP010000000
VERSION     DASTYP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 20554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20554
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643138"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /gene="rpsM"
                     /locus_tag="VFV59_02510"
     CDS             <1..272
                     /gene="rpsM"
                     /locus_tag="VFV59_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU5324732.1"
                     /translation="TAAGVSPDTRVRDLTEDEVNRLREVVDRRFKVEGDLRREVSMNI
                     KRLQEIGSYRGLRHRRNLPVRGQRTKTNARQRRGSKKTVGVRRKK"
     gene            285..680
                     /gene="rpsK"
                     /locus_tag="VFV59_02515"
     CDS             285..680
                     /gene="rpsK"
                     /locus_tag="VFV59_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU5324733.1"
                     /translation="MAERKRAQRGKKRERKNVPAGHAHIQSTFNNTIVTITDPGGNVI
                     SWGSAGLVGFKGSRKSTPYAAAQTADSAAKRAMEHGMRQIEVFVKGPGGGREQAIRSL
                     QSAGLEVTAITDVTPIPHNGCRPPKRRRV"
     gene            697..1323
                     /gene="rpsD"
                     /locus_tag="VFV59_02520"
     CDS             697..1323
                     /gene="rpsD"
                     /locus_tag="VFV59_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5324734.1"
                     /translation="MARYNDAVCRICRREGLKLFLKGSRCYSRKCAFERRSTPPGMNT
                     MRRRKVSEFGLQLREKQKLRKSYSVLERQFRNYFEKAEQRKGMTGENLLRMLEMRLDN
                     VVYRMGFASSRAQARQLVSHGHFAVNGRPTNIPSFAVKVGDRVEVRESRRGREFFKTA
                     GDTMRSAQIPEWVSVDPTKLSGSVVAEPAREQMPLEFNEQLVVEYYSR"
     gene            1340..2377
                     /locus_tag="VFV59_02525"
     CDS             1340..2377
                     /locus_tag="VFV59_02525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5324735.1"
                     /translation="MIELETPQQPAPVTPRIDEVQTEGNLSRFEVTPLQEGYGVTLGN
                     ALRRVLLSSLEGAAVTSIQINGVFHEFSTIENVKEDVTQIVLNVKKLRLRSFARHALT
                     LKLVKRGAGPVTAADINESADVEIVNPDLVLLTLDSDSGSIEMDLTVETGMGYRPAEH
                     TEEMPIGVIPVDAIFSPVRRVNYQVEKTRVGQMTNFDKLSLEIETDGTTTAQAALSEA
                     AEILVREFSRFAEIGRPSTGGGADEVPTAPSANLLDAPIEELDLPMRAYNSLKRNSIT
                     KIGQVLSLSDEEFLRMRNFGQKSLDELKERLALRGFISPEQASMSTDESDLPASAELD
                     EAAAAELDRES"
     gene            2382..2735
                     /gene="rplQ"
                     /locus_tag="VFV59_02530"
     CDS             2382..2735
                     /gene="rplQ"
                     /locus_tag="VFV59_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5324736.1"
                     /translation="MPHRVTGRKLSRTDDHRTAMLANLAVAVLRYERVKTTEAKAKEV
                     RGLVDGMITLAKRGDLHARRQLVSRMPHEPLIVDKLMGEIATKYAERSSGFTRIVKLG
                     QRAGDAAPIVQIELV"
     gene            2770..3627
                     /gene="truA"
                     /locus_tag="VFV59_02535"
     CDS             2770..3627
                     /gene="truA"
                     /locus_tag="VFV59_02535"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5324737.1"
                     /translation="MPADARCARAVIEYDGTAFAGSQVQPNARTVQGELEEALNRLIG
                     ERIRVRLAGRTDAGVHATGQVAAFCLPRRGRGGGWRWAELRARLNAVLPDDLAVRSLG
                     PAPAGFDPRRDARWRVYRYRIRMAGEVRPLDRHRTLEIDDRLDVAAMQAAADRMVGER
                     DFASLGADTHGRTVRHLAEVRVTRRGNLVEVRVTANAFLRRMVRSIVALLLEAGRGGL
                     APGDVERLLAAGERALDGRAAAARGLTLERVVYGRSAMETEESNEGRGGTAEGRGRST
                     TGMTGEQGT"
     gene            3624..4052
                     /gene="rplM"
                     /locus_tag="VFV59_02540"
     CDS             3624..4052
                     /gene="rplM"
                     /locus_tag="VFV59_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5324738.1"
                     /translation="MKTYAVKASEIERSWWVVDATGQTLGRLATRIATLLEGKHKPIY
                     SPHLDTGDHVVVVNAGKIRVTGDKLRQKSYVRHSNYPGGLREESLAALLERKPELVIE
                     RAVKGMLPQNRLGRAMFKKLKVYSGADHPHQAQQPTAVEL"
     gene            4067..4459
                     /gene="rpsI"
                     /locus_tag="VFV59_02545"
     CDS             4067..4459
                     /gene="rpsI"
                     /locus_tag="VFV59_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5324739.1"
                     /translation="MSTNYVYGTGRRKTAVARVRLLPGDGGIVVNGKPAGEYFGGTLR
                     DGSLQLPFRVTETEGRYSASILVAGGGVSGQIGAIRHGIARALVSAHPDARPALREAG
                     LLTRDPRAKERKKYGLKRARKAPQYTKR"
     gene            4561..5496
                     /gene="cdaA"
                     /locus_tag="VFV59_02550"
     CDS             4561..5496
                     /gene="cdaA"
                     /locus_tag="VFV59_02550"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEU5324740.1"
                     /translation="MIDAIREFFDTYLRGSWTSIIDIALVAFVIYWLFILIRGTRAVR
                     IVIGLSILYLVYLAAVFFGLDLLRRLMETAAVVGLFAVVVVFQPELRRALEQIGRIGS
                     VNRFFVSAEVASAERVAREISRAARILAGSRHGALIVLERETGLQDLATESGVPVHAD
                     LRAELLVTIFYRGTALHDGAVIVAGEKITSAGVLLPLSQNVLDSERYGTRHRAAIGIS
                     EQTDAVVIVVSEETGSISVVVRGRIERNLTEEQLRRRIFNLIRPQAPRRAVPFLRRTL
                     EGRWQVGRGEEPEAGPIDPDARVVGGRTESRPVRR"
     gene            5500..6741
                     /locus_tag="VFV59_02555"
     CDS             5500..6741
                     /locus_tag="VFV59_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEU5324741.1"
                     /translation="MGWIFRNWHLKLSAVLLATVLYTGLVFSGSFSESTIQVRIGQEN
                     ASSDVFVLTGDLGLVEVTYRTARDTQQTVVADSFSARVDLADYDMDRAPEPQELPITV
                     TAADGIQILSIDPKFVRVSLDRVETRTVPVVVDPGSVPDGLRIGDPAVDPTEVQVRGP
                     SSVVSLVDRVVAYVTIPASGIDFNEPVNPKPVDVEGQPVGVGLLEVQPESVSVTIDVS
                     QVETTDTITVRPVIEGTPAPGFALEAISVDPSTVTLRGLPEDLEPITEVLTEPISIAG
                     LSADDTIDVDLVVPETVELVDGTAGSVTVSVTIVPSVSSRVFVLGVVCSGAGANACLP
                     GLDQVSVTVTGPGQVLSGLTAAQLTPTLNVAGLAPGSYTINPTIAGLPEGVELLGISP
                     PTVPVQIVAPATPTPAPTPAP"
     gene            6741..8069
                     /gene="glmM"
                     /locus_tag="VFV59_02560"
     CDS             6741..8069
                     /gene="glmM"
                     /locus_tag="VFV59_02560"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU5324742.1"
                     /translation="MGSRFGTDGIRGEVGTEITADLAFRLGHAVGTKFGGVGRSVVLG
                     RDTRRSGEMLSAALGAGLKATGTDVTDLGIVTTPCLVHASAAGPFVAGIMVSASHNPA
                     PDNGLKVVLDGVKADDAAEEALEGLIDSDDARVTLRNHELGRVRPDRGSIEAYRRHLA
                     EAAGDAFSGLRVGIDCANGSASAIAPDLFRALGAQVTVLFDAPDGVNINDGCGSTHPE
                     RLAETVAAARLDLGLAFDGDADRLIAIDERGEVVDGDAIMGICALERLAEGTLRNSVL
                     VATVMSNGGLERAVAAAGGRMVRTPVGDRHVLEAMHREGGILGGEQSGHIIFRDRATT
                     GDGLLTAIELVRSVRAADRPLSELAAAIPRLPQVVLNSAVRRKDEWEADAVFADAVQA
                     AVRQLADRGRILVRPSGTEPKIRIMVEGDDVDEIEGIARELATLAEARLN"
     gene            8097..9926
                     /gene="glmS"
                     /locus_tag="VFV59_02565"
     CDS             8097..9926
                     /gene="glmS"
                     /locus_tag="VFV59_02565"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5324743.1"
                     /translation="MCGIVGYVGPRDAAPILLEGLRRLEYRGYDSAGIAILTESGNVF
                     VEKKAGKLANLTDHLNGDAPAGHPGIGHTRWATHGPPNDTNAHPHVDCTGKLALIHNG
                     IIENYSEIKKRLAAGGHRFTSDTDTEVLAHLIEEKYSGDLVEAVRLALADVRGAYAIG
                     VMHTDHPDRIVGARMNVPLIVGLGDGEGFLASDVPAILEHTKNVVILHEGDIADVTPE
                     RTRILGLDGSEVERDVTQIHWNIEAAEKGGFPHFTLKEIYEQPHAIQECLRGRVSGSG
                     VVHLEELDPIADRLREIERIYVVGCGTASYAAQVGAYLLQDWTGIPAVMQVGSEMRYS
                     PPPIDERTLVIGVSQSGETADTLAPLKMASEKGALIAVVTNVVGSAITRGADAVCYLQ
                     AGPEIAVASTKAFVTQVLVLQMMAMQVAELRGRMSEHRARILGQALRTLPRLAAETLK
                     LGPQIKKLARRYANVRDFIYIGRGLGFPIAMEGALKLKELSYVHAEGYAAGELKHGPI
                     ALLDPETPLVAIATKGSLYEKVVSNVAEARARSAPIIAIATEGDDHIDHYAHDVLHVP
                     ETPEEINPVIAVLPLQLLAYEIAVARGTDVDQPRNLAKSVTVE"
     gene            9961..10338
                     /gene="acpS"
                     /locus_tag="VFV59_02570"
     CDS             9961..10338
                     /gene="acpS"
                     /locus_tag="VFV59_02570"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU5324744.1"
                     /translation="MSRHDIGIDLIDIDRIVTVLGRFPDRFRLRVLTESEQRYCGRKP
                     HRIAGRWAAKEAISKVLGLGVRGVGWREIEILPNRAGAPQVSLHGRAARRAVALDLDE
                     VSVSISHERHLAVAVAVAHRRHD"
     gene            10350..11255
                     /locus_tag="VFV59_02575"
     CDS             10350..11255
                     /locus_tag="VFV59_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5324745.1"
                     /translation="MTATTPITPRWVAARLPERPDRAHKGTFGSVLVVAGSFRYPGAA
                     LLAGLGAMRAGAGLVQVAIGESMVGHLAGSVPELTWLPLDEEGPGLVAPAGWRTITAA
                     AAERDAIVVGPGLGRQPAGQRRARQLVEAMERPCVVDADGLNALSAVPRWWEGVRGDL
                     VLTPHPGEFARLTGAATLDADDDDARASAASQAAARFRQVVVLKGAATVIAAPDGRIM
                     VSTVATPALATAGSGDVLAGAIGALLAAGVAPFEAAVCGVALHGTAGTLAEARIGRAG
                     VLARDIAALLPAAATRLRNPEPEDR"
     gene            11252..12382
                     /gene="alr"
                     /locus_tag="VFV59_02580"
     CDS             11252..12382
                     /gene="alr"
                     /locus_tag="VFV59_02580"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5324746.1"
                     /translation="MTEPGSPHRAWIEVDHAAIRQNLRVVRDLSPGASVIGVVKANAY
                     GHGDVEVARTLVAAGVERLAVATVDEAAGLRDARIEVPLLVLWGIGPTEADEIVAHRL
                     EPIVYDERTIGWLEAAAERADAAVRVHLKVDTGLGRQGAMPEDALSLAARIAASPRLT
                     LAGTMSHLAVPGEDDAYTDVQLLRLARTLDAMRSAGIAPGLVHVSATGDIVAEVVRMV
                     DAVRPGLALYGMLPSWVTERDMGLRPALSLKARPLRLFDLDAGEAIGYGLRFRATRPT
                     RIATLGIGYADGWPRVHANNGAVLVRGQRAPIVGAVSMDGLTVDIGGVAGVTYEDEFV
                     LIGEQGGARITADEVAGERRTINYEVTTALRRRLPRLHLGAG"
     gene            12416..12937
                     /locus_tag="VFV59_02585"
     CDS             12416..12937
                     /locus_tag="VFV59_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEU5324747.1"
                     /translation="MAAPIEIDVWQGGIAELEVDALVVGATESLFMNAGAAAAVARHG
                     GVEIERAAVEQGPIAPGAAVATTAGTLAAGYVIHAVAVGHDRRADPDQLRRAVRSALA
                     FVPPLQLRRVAFSLLGTEHGAFDPAEAAEILVVTLHESAIEPLESVVVATANVAETRA
                     VAAVLAAHRAGIR"
     gene            12934..13194
                     /locus_tag="VFV59_02590"
     CDS             12934..13194
                     /locus_tag="VFV59_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324748.1"
                     /translation="MTDDAAGRLARALDDRGLTAPARLLAESHRPIAPLLSDLGAAVG
                     PLLRAIGGSRVAGISRLVAEEDAVDRLTRSLDELEPPRAKPR"
     gene            13178..14029
                     /locus_tag="VFV59_02595"
     CDS             13178..14029
                     /locus_tag="VFV59_02595"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEU5324749.1"
                     /translation="MPSPAELVGGILGFISEVLNASSLRFEVGPTLVVLGILLVLLQL
                     VARPVTRWTSGDAGGLGAIGRAMALAAESGTDAVVSVGAAGIARSTDALARLQTLAAM
                     PVLDHVARAAARSGVPLRVLTNDPLAGFVAEATIDAAHRRTSTEERVGRSRVVMVGEG
                     REAAAGLPLTARARPAAAIAVGSLREEAQLHLAGLRGTAGSLTVGTAEASQAGTVLLA
                     SHDGLVGPEPFQVAADLGAVVEERTAVTAANRLIALAVLVVIVVTALALLEVVDLAPF
                     LLGTTGA"
     gene            14026..14643
                     /locus_tag="VFV59_02600"
     CDS             14026..14643
                     /locus_tag="VFV59_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324750.1"
                     /translation="MRRATIPALVVVVGLLLLVDYLVVNASLGELAGIVVDAAILVSA
                     GAALAAVAALAIRRGGDLWRRRGDPIGALLVLVGMAAVLLAGLRPGATGASDPAVLWL
                     LNALLVPLGASLFGLLFVSTLLAFQRSISARSREAIVMAVAALAVLVLLLPVGGSVGS
                     WLDGAAAWALAVPLGGVFRGLLLGIAILGAVVAARTMLGIGAGDE"
     gene            14636..15484
                     /locus_tag="VFV59_02605"
     CDS             14636..15484
                     /locus_tag="VFV59_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324751.1"
                     /translation="MSDRLRAVRLAPLLLVLLLGAAWLAPSVGWLRLASSSTVDADRM
                     TGVLDALPPRPIVLVGFDPDLGTYAEIRPTVRALLADLLYRQASVAIVSVTPEGRALA
                     LAELERLRRGTVNQRRILDLGFVPGAEAALVRLTRGGLPIEPAGALAAALASEGIAAV
                     QAIVVVGGNDLGPRSWVEQVQPRLPDRPLVAVAPTVLLPELRPYLGTGQLDALLGTPR
                     DGAAYRATLDVERLDRFLEPREPSRTGLFAGLLVAMGVLIAALLRRGGRPDADRAAPA
                     PGTEAA"
     gene            15481..16125
                     /locus_tag="VFV59_02610"
     CDS             15481..16125
                     /locus_tag="VFV59_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324752.1"
                     /translation="MSGLFGATPADTVATWVAAAATLVVLGGMLGERRLFAWSQHLLA
                     GLATGFLALVVITEVLVPRVVEPLLADPGGRIELWLALALVGAAAGAPWLPRIVGAVP
                     VSIAIGALAAFALGGAVIGTLLPQLVAVSVRGGGDVATTVAAIGAGVITVLVLLSFLR
                     GVPRGRLLRPAASTGRWLLLAGIGGWFGYLLFSRLILLLDRIGFLLGEWLRIGA"
     gene            16122..17756
                     /locus_tag="VFV59_02615"
     CDS             16122..17756
                     /locus_tag="VFV59_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEU5324753.1"
                     /translation="MTSVEAELALLLDVGSAWAKASAIGVVRGRWRLVAHASQPTAWG
                     AAELRRSLVDRMAEAVDARLVDRLDELVARAPRIECHTARAPRRLVVVAVSRELSGGA
                     ARRAAEAAGWSVSDVVSLDDGRSLADRLSLLQQAEVDAWLLAGGFDGTRSPRALEAAA
                     LAAAARRPDGGPVVWAGAAELTAEVEALFEPGAVTSVANPRPDGRRETPDALRAHLQD
                     LIAAVGGGEPETNLASMSFPRAVGALAARSRLRVLAVDVGARAAVRALAERDGTVTSR
                     VHAQGGLAGLAAVPGAASRVARLAGDAGDEAAVADLLQTLAARAATLPETAEELSAMH
                     VAARLQAAALIEEGGVGPLDLLIGAGRTIAAAPHPAQALRILLDGVRPVGVTQLALDA
                     AAALGPLGSLPDAEIGEGIGLLADDLLVPLGTTVVTRGGDPGRLAMRVTVHRSGWPAS
                     APIELRVGQLQVVPLPPGQAAELSIEVGDGVSIGAPRRSPRITATATGGTVGLVLDAR
                     GVPVQLPRRADDRRAVLAGWRDALGRETSSTAERLA"
     gene            17753..18784
                     /locus_tag="VFV59_02620"
     CDS             17753..18784
                     /locus_tag="VFV59_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324754.1"
                     /translation="MSAVHRVRLPVGATVLVEAGHRVEPAEVLATRRPPGAGVTIPVA
                     VPLRRSAADAREALEVTPGSVLEADALLARQGSREVRVPDACLFLAYDADDGTALIAP
                     LGATEPILGHVRGEVASVAADAIEIRVDGALVTGIAGSGAAVHGELRVAVHDPGEELR
                     AGAIDVGATGRILVGGSRASAETLTRARAMGVAGIVLAGALDKELRDFDAAQRRRRQI
                     GGARGAFSILLLEGYGKTSFDPALFAWFRAHDGRMASLFGDGARLYVYDAEPVPGRRA
                     LPRPGDRVIAHRRPSAGATGVLVREVPASYAAASGVAARSALVRFDDARTAIVPLANL
                     EAAEPRSEA"
     gene            18810..19304
                     /gene="tsaE"
                     /locus_tag="VFV59_02625"
     CDS             18810..19304
                     /gene="tsaE"
                     /locus_tag="VFV59_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5324755.1"
                     /translation="MDPATLTPHASGTRSTRAPEETRALGRAIGQAAPAGTVLALIGE
                     LGAGKTQLAKGVAEGLGVRSVVNSPTFVLMNEHAGRLRLYHIDAYRLGDPEEAVAAGL
                     LDERQADGVAVVEWADRLEGWLPDDRLEIALEGDGVDADHRRITWRAFGAQHQRLAAE
                     ALHA"
     gene            19301..19939
                     /gene="tsaB"
                     /locus_tag="VFV59_02630"
     CDS             19301..19939
                     /gene="tsaB"
                     /locus_tag="VFV59_02630"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5324756.1"
                     /translation="MIIAIESASTDLSLALAEPDGTLIADAAWTSRQRQSAELLPHLL
                     QLLDQSGHRLDQATALAVGLGPGSFTGLRVAMSLGKGLALSLGRPLVGVPSLEAWLAS
                     DAAATAAVARAGASEAYVLRPADAAPHIVERDRLAEIIGDHPVVPDELAAAFELGSSA
                     SPRRAAAALARLAAERLAAAPEGDDLRTIEPIYLRAPRGVHDTPNEGRVTWL"
     gene            19930..20442
                     /gene="rimI"
                     /locus_tag="VFV59_02635"
     CDS             19930..20442
                     /gene="rimI"
                     /locus_tag="VFV59_02635"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5324757.1"
                     /translation="MALTARVVVDPMRLDDVRAVHEIERLSFATPWPAYAFEQELKGN
                     RLARYVVARAGERVVGFAGIWLMVDEAHVTTFGVHPDWRRQGIGRQLMLNVAELSLAI
                     GARRMTLEVRVSNEAAQGLYRSFGFEVVGKRPHYYTDDGEDALVMTTPALREPHMRQI
                     VAAQRQLIGT"
     gene            20439..>20554
                     /locus_tag="VFV59_02640"
     CDS             20439..>20554
                     /locus_tag="VFV59_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5324758.1"
                     /translation="MSAPRILAIESSCDETGVAVVVGGRRIEANQVATQVAL"
BASE COUNT         2770 a         7355 c         7635 g         2794 t
ORIGIN      
        1 tcactgcggc cggcgtctcg ccggacacca gggtccggga cctgaccgag gacgaggtca
       61 accggctgcg cgaggtggtg gaccggcgct tcaaggtcga gggtgacctg cgccgcgagg
      121 tgagcatgaa catcaagcga ctccaggaga tcggctcgta tcggggcctc cgccatcggc
      181 gcaatctgcc cgtccgcggg cagcgaacga agacgaacgc gcggcagcgc cgtgggtcga
      241 agaagacggt cggcgtccgc cgcaagaagt gatcagggag tttcatggca gagcgtaagc
      301 gcgcccagcg aggcaagaag cgcgagcgga agaacgtgcc ggccgggcac gcgcacatcc
      361 agtcgacgtt caacaacacg atcgtcacga tcaccgatcc cggcggaaac gtgatcagct
      421 ggggcagcgc ggggctcgtc ggcttcaagg gctcgcgcaa gagcacgccg tacgcggccg
      481 cgcagacggc cgactcggcg gccaagcgcg ccatggagca cggcatgcgg cagatcgagg
      541 tcttcgtcaa gggccccggc ggcggccgag agcaggccat ccgctcgctg cagtccgccg
      601 gcctcgaggt gaccgcgatc accgacgtca cgccgatccc acacaacggc tgccgcccgc
      661 cgaagcggcg ccgggtctga gggggagcga accgacatgg cacgttacaa cgacgccgtc
      721 tgccgcatct gccgtcgcga ggggctgaag ctcttcctga agggcagtcg ctgctacagc
      781 cgaaagtgcg ccttcgagcg ccgcagcacg ccgccgggca tgaacaccat gcggcgccgc
      841 aaggtgagcg agttcgggct gcagctgcgc gagaagcaga agctgcgcaa gagctactcg
      901 gtcctcgagc gccagttccg caactacttc gagaaggccg agcagcgcaa gggcatgacc
      961 ggcgagaacc tgctccgcat gctcgagatg cgcctcgaca acgtcgtcta ccgcatgggc
     1021 ttcgcgtcca gccgcgccca ggcgcgccag ctggtcagcc acggccactt cgccgtcaac
     1081 ggccgcccga ccaacatccc gagcttcgcg gtgaaggtcg gcgatcgcgt cgaggtgcgc
     1141 gagtcgcggc gcggtcgcga gttcttcaag acggccggcg acacgatgcg atcggcccag
     1201 atccccgagt gggtgagcgt cgacccgacc aagctgtcgg gatcggtcgt cgccgaaccg
     1261 gcgcgcgagc agatgccgct cgagttcaac gagcagctcg tcgtcgagta ctactcgcgc
     1321 taggagtcac acccttccca tgatcgaact cgaaactccg cagcagccgg ccccggtgac
     1381 cccgcgcatc gacgaggtgc agaccgaggg gaacctgagc cgcttcgagg tcacgccgct
     1441 ccaggagggc tacggcgtca cgctgggcaa cgccctgcgt cgcgtgctcc tgagctccct
     1501 cgagggcgcg gccgtcacca gcatccagat caacggcgtc ttccacgagt tcagcacgat
     1561 cgagaacgtc aaggaggacg tcacccagat cgtgctgaac gtgaagaagc tgcggctgcg
     1621 ctcgttcgcg cggcacgcgc tcacgctcaa gctcgtcaag cgcggcgccg gcccggtcac
     1681 cgcggccgac atcaacgaat ccgccgacgt cgagatcgtc aaccccgacc tcgtgctcct
     1741 caccctcgac tcggattccg ggtcgatcga gatggacctg acggtcgaga ccggcatggg
     1801 ctaccgcccg gccgagcaca ccgaggagat gccgatcggc gtcatcccgg tggatgcgat
     1861 cttctccccg gttcgccggg tcaactacca ggtcgagaag acccgcgtcg gccagatgac
     1921 gaacttcgac aagctgtcgc tcgagatcga gaccgacggc acgaccacgg cgcaggcggc
     1981 gctgagcgag gcggccgaga tcctggtccg cgagttcagc cgcttcgcgg agatcggccg
     2041 gccgtccacg ggtggcgggg ccgacgaggt gccgaccgcg ccgagcgcga acctgctcga
     2101 cgcgccgatc gaggagctcg acctcccgat gcgggcctac aacagcctca agcgcaactc
     2161 gatcaccaag atcggccagg tcctgtcgct cagcgacgag gagttcctgc gcatgcgcaa
     2221 cttcgggcag aagagcctgg acgagctgaa ggagcgcctc gcgctgcgtg gcttcatcag
     2281 cccggagcag gcctccatga gcaccgacga gtccgacctg ccggccagcg ccgagctgga
     2341 cgaggccgcg gccgccgagc tcgaccggga gtcctgagcc gatgccgcac cgcgtcaccg
     2401 ggcgcaagct cagccggacc gacgaccacc gcaccgcgat gctggccaac ctggccgtcg
     2461 ccgtgctccg ctacgagcgc gtgaagacca ccgaggcgaa ggccaaggag gtccgcgggc
     2521 tggtggacgg gatgatcacg ctcgccaagc gcggcgacct ccacgcgcgg cggcagctcg
     2581 tgagccgcat gccgcacgag ccgctcatcg tcgacaagct gatgggcgaa atcgcgacca
     2641 agtacgccga gcgcagctcc ggcttcacgc gcatcgtgaa gctcggccag cgcgccgggg
     2701 atgcggcccc gatcgtccag atcgagctcg tctgagcgac ggcgcgcaca acgacatacc
     2761 gaacgaccga tgccggccga tgcgcgctgc gccagggcgg tgatcgagta cgacgggacg
     2821 gccttcgccg gctcgcaggt ccaaccgaat gcgcggaccg tgcaggggga gctggaggag
     2881 gcccttaaca ggctgatcgg cgagcggatt cgcgtccgtc tcgccgggcg aaccgacgcc
     2941 ggggtgcatg ccaccggcca ggttgccgcc ttctgtctcc cgcgccgcgg tcgcggtggg
     3001 gggtggcggt gggcggagct ccgcgcacgg ctgaacgccg tgttgccgga tgacctggcg
     3061 gtgcggtccc tcgggccggc gcccgcgggc ttcgatccgc ggcgggacgc ccggtggcgt
     3121 gtgtaccgct accggatccg gatggccggc gaggtgcggc cgctggaccg gcatcggacg
     3181 ctcgagatcg acgaccggct ggacgtcgcc gccatgcagg cggcggcgga tcggatggtc
     3241 ggggagcgcg acttcgcctc cctgggcgcc gacacgcatg gccggacggt gcggcacctg
     3301 gccgaggtgc gcgtgacccg tcgcgggaac ctcgtggagg tccgcgtgac ggccaacgcg
     3361 ttcctgcgcc ggatggtgcg cagcatcgtg gccctcctgc tggaggccgg ccgaggaggc
     3421 ctggcgccgg gggacgtcga gcggctgctg gccgccggcg agcgggccct cgacgggcgc
     3481 gcggcagcgg cacgcgggct cacgctcgag cgcgtcgtgt acggcagatc agccatggaa
     3541 accgaagaat cgaacgaagg acgaggcggc accgccgagg ggcgaggccg cagcaccaca
     3601 ggcatgaccg gagagcaggg aacgtgaaga cctacgccgt caaggcgagc gagatcgagc
     3661 gcagctggtg ggtcgtggac gcgaccggcc agaccctcgg ccggctggcg acacgcatcg
     3721 ccaccctgct ggagggcaag cacaagccga tctactcgcc gcacctcgac accggcgacc
     3781 atgtggtggt cgtcaacgcg ggcaagatcc gcgtcacggg cgacaagctg cgacagaaga
     3841 gctacgtccg ccactccaat taccccggcg gcctgcgcga ggagagcctc gccgcgctgc
     3901 tcgagcgcaa gccggagctc gtgatcgagc gcgcggtgaa ggggatgctg ccccagaacc
     3961 gcctcggtcg cgcgatgttc aagaagctga aggtgtacag cggcgcggac cacccgcacc
     4021 aggcccagca gccgaccgct gtcgagctgt aacaggaagg acgatcgtga gcacgaacta
     4081 cgtctacggg acgggcaggc gcaagaccgc cgtcgcgcgc gtccgcctgc tgccgggcga
     4141 cggcggcatc gtcgtcaacg gcaagcccgc gggcgagtac ttcggcggca cgctgcgcga
     4201 cggctccctc cagctgccgt tccgcgtcac cgagaccgag ggccgctact cggccagcat
     4261 cctggtcgcc ggcggcggcg tctccggcca gatcggcgcc atccgccacg gcatcgcccg
     4321 cgcgctcgtg agcgcgcatc ccgacgcgcg gcccgcgcta cgcgaggccg gcctgctgac
     4381 ccgcgacccg cgggccaagg agcgcaagaa gtacggcctg aagcgcgctc ggaaggcgcc
     4441 gcagtacacg aagcgctagg aacccatctc gcccgcccaa ccacgagagc ctcggccgga
     4501 ccggccgggg ctctttggta tcatcgggac cctccccgta acggacagcc tgacgacgct
     4561 gtgatcgacg cgatccgcga attcttcgac acgtacctgc gcgggagctg gacgtcgatc
     4621 attgacatcg cgctcgtcgc gttcgtcatc tactggctgt tcatcctgat ccgcggcacg
     4681 cgcgcggtgc ggatcgtcat cggcctctcg atcctgtacc tggtctacct ggccgccgtc
     4741 ttcttcggcc tcgacctgct gcgccggctg atggagacgg ccgcggtggt cggcctcttc
     4801 gcggtcgtgg tcgtgttcca gccggagctg cgacgggcgc tcgagcagat cggccgcatc
     4861 ggctccgtca accgcttctt cgtcagcgcc gaggtggcca gcgccgagcg ggtggcgcgc
     4921 gagatcagcc gggcggcacg gatcctcgcc ggatcgcgac acggggcgct gatcgtcctc
     4981 gagcgcgaga ccggcctgca ggacctcgcg acggagtccg gggtgcccgt tcacgccgac
     5041 ctgcgggccg agctgctggt caccatcttc taccgcggga cggcgctgca cgacggcgcg
     5101 gtcatcgtgg cgggagagaa gatcacctcg gccggcgtgc tgctgccgct cagccagaac
     5161 gtgctcgact cggaacggta cggcacccgg caccgggccg ccatcggcat cagcgagcag
     5221 accgatgccg tcgtcatcgt ggtcagcgag gagaccggct cgatcagcgt ggtcgtgcgc
     5281 ggccgcatcg agcgcaacct gaccgaggag cagctgcggc ggcgcatctt caacctgatc
     5341 cgtccgcagg cgccgcgccg cgccgttccg ttcctgcggc gcacgctgga ggggcgctgg
     5401 caggtgggcc ggggcgagga acccgaggcc ggtcccatcg atcccgacgc gcgggtcgtg
     5461 gggggccgca ccgagagtcg gccggtgagg cgctgacgca tgggctggat cttccgtaac
     5521 tggcacctca agctctcggc cgtcctgctg gccaccgtgc tgtacaccgg gctcgtcttc
     5581 tccggctcat tcagcgagtc gaccatccag gtccggatcg gtcaggagaa cgcctcgagc
     5641 gacgtgttcg tgctgaccgg tgacctgggc ctggtggagg tgacctaccg cacggcccgc
     5701 gacacccagc agacggtggt ggccgactcg ttcagcgccc gcgtcgacct ggccgactac
     5761 gacatggacc gcgcgcccga gccgcaggag ctgccgatca cggtgacggc agccgacggc
     5821 atccagatcc tctcgatcga ccccaagttc gtgcgcgtct cgctcgatcg ggtggagacg
     5881 cgaaccgtgc ccgtggtcgt tgaccccggc tccgtcccgg acgggctgcg gatcggcgat
     5941 ccggcggtcg accccacgga ggtccaggtg cgtggcccct cgtcggtcgt ctcgctggtg
     6001 gaccgcgtgg tcgcctacgt cacgattccg gcgtcgggca tcgacttcaa cgagccggtc
     6061 aaccccaagc cggtcgacgt ggagggtcag ccggtcggtg tcgggttgct ggaggtgcag
     6121 ccggagagcg tctcggtcac gatcgacgtg tcgcaggtgg agacgaccga caccatcacc
     6181 gtgcgcccgg tgatcgaggg cacgcccgcc ccgggcttcg cgctggaggc gatcagcgtc
     6241 gatccgtcga ccgtcacgct gcgcggcctg ccggaggacc tcgagcccat cacggaggtg
     6301 ctgaccgagc cgatcagcat cgccggcctc tcggcggacg acaccatcga cgtcgacctg
     6361 gtcgtccccg agaccgtcga gctcgtcgac ggcacggctg gcagcgtcac ggtcagcgtc
     6421 accatcgtcc cgtcggtctc cagccgggtc ttcgtgctgg gcgtcgtgtg cagcggcgct
     6481 ggcgcgaacg cctgcctgcc agggctcgac caggtgtccg tgacggtcac cggtccgggc
     6541 caggtgctga gcgggctcac cgccgcccag ctcacgccga cgctcaacgt ggcgggcctc
     6601 gcccccggct cgtacacgat caacccgacc atcgccgggc tgccggaggg cgtcgagctg
     6661 ctcggcatct ccccgccgac ggtcccggtc cagatcgtgg cgccggccac gccgacaccc
     6721 gcgccgacgc cggcgccgtg atggggagtc gattcgggac cgacggcatc cgcggcgagg
     6781 tcggcacgga gatcacggcc gatctcgcct tccggttggg ccatgcggtc ggaaccaagt
     6841 tcggcggcgt cggccgctcc gtcgtcctgg ggcgcgacac gcgacgctcg ggcgagatgc
     6901 tgagcgccgc gctcggcgcc gggctgaagg ccacgggcac cgacgtcacc gacctgggga
     6961 tcgtcacgac cccctgcctc gtgcatgcgt ccgcggcggg gccgttcgtg gccggcatca
     7021 tggtgtccgc ctcccacaac ccggcgccgg acaacgggct gaaggtcgtc ctggacggag
     7081 taaaggccga cgatgcggcc gaggaggcgc tcgaggggct gatcgactcc gacgatgcgc
     7141 gcgtcaccct ccgcaaccac gagctcggcc gcgtgcgccc cgaccgcggc tcgatcgagg
     7201 cctaccgacg gcacctggcg gaggccgccg gcgacgcgtt ctcagggctg cgcgtcggca
     7261 tcgactgcgc caacggctcg gccagcgcaa tcgccccgga cctgttccgc gcgctgggcg
     7321 cccaggtcac cgtgctgttc gatgcgcccg acggcgtgaa catcaacgac ggctgcggct
     7381 cgacccaccc cgagcgcctg gccgagaccg tggccgcggc ccggctcgat ctcggcctcg
     7441 cattcgacgg cgacgcggat cggctgatcg ccatcgatga gcgcggcgag gtggtagacg
     7501 gcgacgcgat catggggatc tgcgccctcg aacgcctggc ggagggaacg ctgcgcaaca
     7561 gcgtcctggt ggcgaccgtc atgagcaacg gcggcctgga gcgcgccgtc gccgcagcgg
     7621 gaggcaggat ggtccggaca ccggtcgggg atcgccacgt cctcgaggcg atgcaccgcg
     7681 agggcggcat cctgggcggg gagcagtccg gccacatcat cttccgcgac cgcgccacca
     7741 cgggcgacgg gctcctcacc gccatcgagc tggtgcggtc cgtgcgcgcg gccgatcggc
     7801 cgctgtccga gctggcggcc gccatcccga ggctgccgca ggtggtgcta aactcggccg
     7861 tccgccgcaa ggacgagtgg gaggcggacg ccgtcttcgc cgatgccgtg caggccgccg
     7921 tccggcagct ggccgaccgg ggacggatcc tcgtccgccc atccggcacc gagcccaaga
     7981 tcaggatcat ggtcgagggc gacgacgtcg acgagatcga aggaatcgcc cgcgagctgg
     8041 ccacgctcgc cgaggcacga ctcaactagc gccggccgcc ggcgaggagg ttccccatgt
     8101 gcggcatcgt cgggtacgtc ggaccgcgtg acgctgcgcc gatcctgctg gagggcctgc
     8161 gtcgcctcga gtaccgcggc tacgactccg ccggcatcgc gatcctcacc gagtcgggca
     8221 acgtcttcgt cgagaagaag gccggcaagc tggccaacct gacggaccac ctcaacggcg
     8281 acgctccggc cggccatccc ggcatcggtc acacgcgctg ggcgacccac gggccgccga
     8341 acgacaccaa cgcccacccc cacgtcgact gcaccggcaa gctggccctg atccacaacg
     8401 ggatcatcga gaactacagc gagatcaaga agcgcctcgc ggccggcggg catcggttca
     8461 cctccgacac ggacaccgag gtcctggccc acctcatcga ggagaagtac tccggcgacc
     8521 tggtggaggc cgtgcgcctg gccctcgccg atgtccgcgg cgcgtacgcc atcggcgtca
     8581 tgcataccga ccacccggac cgcatcgtcg gggcgcggat gaacgtgccg ctcatcgtcg
     8641 gcctcgggga cggggaaggc ttcctggcca gcgacgttcc ggcgatcctc gagcacacca
     8701 agaacgtggt gatcctgcac gagggcgaca tcgccgacgt gacgccggag cggacccgga
     8761 tcctgggcct tgacgggtcg gaggtcgagc gcgacgtcac gcagatccac tggaacatcg
     8821 aggcggccga aaagggcggg ttcccgcact tcacgctcaa ggagatctac gagcagccgc
     8881 acgccatcca ggagtgcctc cgcggtcgcg tcagtgggag tggcgtcgtg cacctcgagg
     8941 agctcgaccc gatcgccgat cggctgcgcg agatcgagcg gatctacgtg gtgggctgcg
     9001 ggacggccag ctatgccgcc caggtcggcg cgtacctgct gcaggactgg acgggcatcc
     9061 cggcggtgat gcaggtcggt tccgagatgc gctacagccc tccgcccatc gacgagcgca
     9121 cgctggtcat cggcgtcagc cagtcgggag agacggcgga cacgctcgcg ccgctgaaga
     9181 tggcatccga gaagggcgcc ctgatcgccg tcgtcaccaa cgtcgtcggc agcgccatca
     9241 cgcgcggcgc cgatgccgtc tgctacctgc aggcgggccc ggagatcgcc gtcgccagca
     9301 ccaaggcgtt cgtgacccag gtcctggtgc tgcagatgat ggccatgcag gtcgccgagc
     9361 tgcgcgggcg aatgtcggag caccgcgcgc gcatcctggg gcaggcgctg cgcacgctgc
     9421 cgcggctggc ggccgagacg ctgaagctgg gcccgcagat caagaagctg gctcggcgct
     9481 atgccaacgt gcgcgacttc atctatatcg gccgtgggct cgggttcccg atcgccatgg
     9541 agggggcgct gaagctcaag gagctcagct acgtccacgc cgagggctac gccgccggcg
     9601 agctcaagca cggaccgatc gcgctgctcg atcccgagac accgttggtc gcgatcgcca
     9661 ccaagggctc gctgtacgag aaggtggtga gcaacgtggc cgaggcgcgc gcgcgctcgg
     9721 ccccgatcat cgccatcgcc accgagggcg acgatcacat cgaccactac gcccacgacg
     9781 tcctgcacgt ccccgagacg cccgaggaga tcaacccggt catcgcggtg ctgccgctgc
     9841 agctgctcgc ctacgagatc gcggttgccc gcggaacgga cgtggaccag ccgaggaacc
     9901 tcgccaagtc ggtcacggtc gagtagggaa gacgcccgcc gtcgggtacg ctgcacggcg
     9961 atgagccggc acgacatcgg gatcgacctg atcgacatcg accgcatcgt cacggtgctg
    10021 gggcgattcc ccgaccgctt ccggctgcgc gtgctcaccg agtccgaaca gcggtactgc
    10081 gggcgcaagc cccaccgcat cgcggggcgc tgggccgcga aggaggcgat cagcaaggtg
    10141 ctcggcctcg gcgtgcgggg cgtcggctgg cgggagatcg agatcctgcc gaaccgcgcc
    10201 ggcgcgccgc aggtcagcct ccacggacgc gcggcgcggc gggccgtggc cctggacctg
    10261 gacgaggtca gcgtctcgat ctcgcacgag cggcacctgg ccgtcgcggt ggccgtcgcc
    10321 caccgtcgcc acgactgagg ccgtctggca tgaccgccac gactccgatc accccgcgct
    10381 gggtcgccgc gcgccttccc gagcgcccgg accgtgccca caagggcacg ttcggctcgg
    10441 tgctggtggt ggccggctcg ttccgctacc ccggcgccgc gctgctcgcc ggactgggcg
    10501 ccatgcgcgc cggcgccggg ctggtccagg tcgcgatcgg cgagtcgatg gtcgggcacc
    10561 tggcgggcag cgttccggag ctcacctggc tgccgctcga cgaagagggc ccggggctcg
    10621 tcgccccggc gggatggcgc acgatcaccg cggcggccgc agagcgggac gcgatcgtgg
    10681 tcgggccggg cctcggccgg cagccagccg gtcaacggcg ggcgcgccag ctggtcgagg
    10741 cgatggaacg gccgtgcgtc gtcgacgcgg atggcctgaa tgcgctgtcg gccgtcccgc
    10801 gctggtggga gggtgtgcgc ggcgacctgg tgctgacgcc gcaccccggc gagttcgcgc
    10861 gactcaccgg ggcggccacc ctcgacgccg atgacgacga cgcgcgggcg tcggcggcga
    10921 gccaggcggc ggcccggttc cggcaggtgg tggtgctgaa gggcgccgcg accgtgatcg
    10981 ccgcgccgga cggtcggatc atggtgtcga ccgtcgcgac gccggcgctc gcgaccgccg
    11041 gtagcggcga cgtgctggcg ggggcgatcg gggcgctgct ggccgccggg gtcgcgccgt
    11101 tcgaggcggc ggtctgcggc gtcgccctcc acggcacggc cggcacgctg gcggaggcga
    11161 ggatcggacg tgccggcgtg ctggcccgcg acatcgccgc gctcctgccg gcggcggcca
    11221 cgcgcctgcg caacccggag ccggaggatc gatgacggaa cccggcagcc cgcaccgggc
    11281 gtggatcgag gtcgaccacg ccgccatccg tcagaacctg cgcgtggtgc gcgacctgag
    11341 ccccggcgcc tcggtcatcg gcgtggtcaa ggcgaacgcc tacggccacg gggatgtcga
    11401 ggtggcgcgc acgctggtgg cggcgggcgt cgagcgattg gcggtcgcga ccgtcgacga
    11461 ggcggctggg ctgcgggacg cgcggatcga ggtcccgctg ctggtcctgt ggggcatcgg
    11521 tcccaccgag gcggacgaga tcgtcgccca tcggctcgag ccgatcgtct acgacgagcg
    11581 cacgatcggc tggttggagg cggccgccga gcgtgccgat gcggcggtgc gcgtgcacct
    11641 caaggtcgat accgggctcg gacgccaggg ggcgatgccc gaggacgcgc tctcgctcgc
    11701 cgcgcgcatc gcggcgtcgc cgcggctgac gctggctggc accatgagcc acctggccgt
    11761 gcccggcgag gacgacgcct acaccgacgt gcagctgctg cggctggccc ggacgctcga
    11821 cgcgatgcgc tcggccggca tcgctccagg gctggtccac gtcagcgcca cgggcgacat
    11881 cgtggccgag gtcgtgcgca tggtggacgc ggtgcgcccc ggccttgccc tgtacgggat
    11941 gctgccgtcc tgggtgaccg agcgcgacat ggggctgcgc ccggccctca gcctgaaggc
    12001 acgccccctc cgcctcttcg acctcgacgc tggcgaggcc atcggctacg gccttcggtt
    12061 ccgcgcgacg cggccgacgc gcatcgccac cctgggcatt ggctacgccg atggatggcc
    12121 gcgagtgcac gccaacaacg gcgcggtgct ggtgcgcggc caacgggcgc cgatcgtcgg
    12181 cgcggtgagc atggacggtc tgaccgtcga tatcggcggg gtggctggtg taacctacga
    12241 ggacgagttc gtcctcatcg gcgagcaggg gggcgcacgg atcacggccg acgaggtggc
    12301 cggcgagcgg cggaccatca actacgaggt caccaccgcg ctgcggcggc gcctaccacg
    12361 gctccacctc ggcgccggtt gacggacgaa gcgaggcacc gagcgacacc gagcgatggc
    12421 agcaccgatc gagatcgacg tgtggcaggg cgggatcgcc gagctcgagg tggacgccct
    12481 cgtcgtcggc gccaccgagt ccctcttcat gaacgccggg gcggccgcag cggtggcgcg
    12541 gcatggcggc gtcgagatcg agcgcgcggc cgtcgagcag ggcccgatcg cgccgggtgc
    12601 ggcggtcgcg acgacggccg gcacgctggc cgcggggtac gtcatccacg ccgtcgccgt
    12661 cggacacgac cggcgcgcgg accccgacca gcttcgccgt gccgtgcggt ccgccctggc
    12721 cttcgtgccc ccgctccagc tgcgccgcgt ggcgttctcg ctgctcggca cggagcacgg
    12781 cgccttcgat ccggcggagg ccgcagagat cctggtcgtc acgctccacg agtcggcgat
    12841 cgaaccgctc gagtccgtcg tcgtcgccac ggccaacgtg gcggagacgc gcgccgtcgc
    12901 ggccgtcctg gccgcccatc gcgccgggat tcgatgaccg acgacgcggc ggggcggctg
    12961 gcgcgcgccc tcgatgaccg cgggctgacc gcgcccgcgc ggctgctggc cgaatcgcat
    13021 cgtccgatcg cgccactgct ctcggacctg ggtgccgctg tcggtccgct gcttcgcgcg
    13081 atcggcggct cgcgcgtcgc aggcatcagt cggctcgtgg ccgaggagga tgcggttgat
    13141 cggttgaccc ggagcctgga cgagctggag ccaccgcgtg ccaagccccg ctgagctcgt
    13201 cggcgggatc ctcggcttca tcagcgaggt gctgaacgcc tcctcgctgc gcttcgaggt
    13261 ggggcccacg ctcgtcgtgc tcggcatcct gctggtcctg ctgcagctgg tcgcacgtcc
    13321 ggtcacgcgc tggacgtcgg gcgacgccgg cgggctcggc gccatcggtc gtgccatggc
    13381 gcttgcggcg gagtccggta ccgacgccgt cgtctcggtg ggcgccgccg ggatcgcgcg
    13441 ctcgaccgat gcgctggcgc gtctccagac gctcgccgcc atgccggtgc tcgatcatgt
    13501 ggctcgggcc gcggcccgca gcggcgtgcc gctccgggtc ctgaccaacg acccgctggc
    13561 cgggttcgtg gccgaggcga ccatcgatgc cgcgcaccgg cgcacctcga ccgaggagcg
    13621 cgtggggcgc tcgcgcgtcg tgatggtggg cgagggacgc gaggccgcgg ccgggctgcc
    13681 gttgaccgct cgggcgcgtc ccgctgccgc catcgcggtc ggcagtctgc gcgaggaggc
    13741 gcagctgcac ctggcgggac tgcgcggaac ggcgggctcg ctcaccgtcg gcaccgccga
    13801 ggcctcgcag gccgggaccg tgctcctcgc ctcgcacgat ggcctcgtcg gcccggagcc
    13861 gttccaggtg gccgccgacc tcggcgcggt ggtggaggaa cgtaccgccg tgacggcggc
    13921 gaaccggctg atcgcgctgg ccgtgctggt ggtgatcgtc gtgaccgcgc tcgcgctcct
    13981 cgaggtcgtt gacctcgccc ccttcctgct cgggaccacc ggcgcgtgag acgcgcaacg
    14041 atcccggcac tggtcgtcgt cgtcggcctg ctcctgctcg tcgactatct ggtcgtcaac
    14101 gcctcgctcg gcgagctcgc ggggatcgtg gtggacgccg cgatcctggt gtcggccggc
    14161 gccgcgctgg ccgcggtcgc cgcgctggcc atccggcgcg gcggggatct gtggcgccgc
    14221 cgcggcgacc cgattggcgc gctgctggtg ctggtcggca tggcggcggt gctcctggcc
    14281 ggactgcgcc cgggcgccac cggcgcgagc gacccggccg tgctgtggtt gctcaacgcg
    14341 ctcctcgtgc cgctgggggc gtcgctcttc gggctgctgt tcgtgagcac gctgctcgcg
    14401 ttccagcgat cgatctcggc acggagccgg gaggccatcg tcatggccgt cgctgccctg
    14461 gcggtcctgg tcctgctgct ccccgtcggt ggatccgtcg gaagctggct cgacggggcc
    14521 gcggcgtggg cgctggccgt cccgctcggc ggcgtcttcc gcggcctgct gctgggcatc
    14581 gcgatcctgg gcgccgtggt ggccgcccgc acgatgctcg ggatcggtgc cggcgatgag
    14641 tgatcggctc cgggcggttc gcctcgcccc gctgctgctg gtcctgctgc tcggcgccgc
    14701 ctggctggcg ccgtcggtcg ggtggctccg cctcgcctcg tcgagcacgg tggacgccga
    14761 ccgcatgacg ggagtgctcg acgccctgcc tcctcgcccg atcgtcctgg tcggcttcga
    14821 cccggacctg ggcacgtacg ccgagatccg gccgacggtc cgggcgctgc tggccgacct
    14881 gctgtatcgc caggcgagcg tggcgatcgt cagcgtgacg ccggaaggcc gggcgctggc
    14941 cctggcggag ctcgagcgcc tgcgccgtgg cacggtgaac cagcgccgga tcctcgacct
    15001 cggcttcgtc cccggcgcgg aggccgcgct cgtgcggctg acgcgcggcg gcctgccgat
    15061 cgagcccgcc ggcgcccttg ccgcggcact cgccagcgag ggcatcgcgg ccgtgcaggc
    15121 gatcgtggtg gtcggcggga acgacctggg accccgatcc tgggtcgagc aggtccagcc
    15181 ccgcctgccg gatcggccgc tcgtcgccgt cgccccgacc gtcctgctgc ccgagctgcg
    15241 cccgtacctg ggcaccggcc agctcgacgc gctgctcggg acgccccgcg acggggcggc
    15301 gtaccgggcc acgctcgacg tcgagcgcct ggaccggttc ctcgagcccc gggagccgag
    15361 ccgcacgggc ctgttcgccg gcctgctggt cgccatgggc gtcctgatcg ccgcgctctt
    15421 gcgacgtggc ggccgacccg acgcggaccg ggccgcaccc gctccgggga cggaggcggc
    15481 atgagcggcc tgttcggtgc cacgccggcg gacacggtgg ccacgtgggt ggcggccgcg
    15541 gcgaccctcg tcgtcctcgg cggcatgctc ggcgagcgcc ggctgttcgc ctggtcgcag
    15601 cacctcctcg ccgggctggc caccgggttc ctcgcgctcg tcgtcatcac cgaggtcctc
    15661 gtgccgcgtg tcgtcgagcc gttgctcgcc gacccgggcg gccgcatcga gctgtggctg
    15721 gcgctggccc tcgtcggcgc ggcggccgga gcgccgtggc tgccgcgcat cgtcggcgcc
    15781 gttcccgtct cgatcgccat cggcgcgctg gccgccttcg cgctcggcgg cgcggtgatc
    15841 ggcaccctgc tgccgcagct cgtcgcggtc agcgttcgcg gcggtgggga cgtggcgacg
    15901 acggtggccg ccatcggcgc cggcgtcatc accgtcctgg tgctcctcag cttcctgcgt
    15961 ggcgttcccc gcggccgcct gctgcggccc gcggcgagca ccgggcgctg gctgctgctg
    16021 gccggcatcg gcggctggtt tggttacctg ctgttctccc ggctgatcct gctgctcgac
    16081 cgcatcggct tcctgctggg tgagtggctg aggatcggcg catgacgagc gtggaggcgg
    16141 agctggccct cttgctggac gtggggtcgg cgtgggccaa ggcgtcggcc atcggggtgg
    16201 tgcgtgggcg ctggcggctg gtggcccacg catcgcagcc gacggcctgg ggcgccgccg
    16261 agcttcgccg ctcgctggtg gatcggatgg ccgaggccgt cgacgcccgg ctggtggacc
    16321 ggctcgacga actggtcgcg cgcgcgccgc ggatcgagtg ccacacggcg cgggcgcccc
    16381 ggcgattggt ggtcgtggcc gtctcgcgcg agctgtcggg tggggcggcg cgccgggcgg
    16441 cggaggcggc cggatggagc gtctcggacg tcgtgagcct ggacgatggg cgcagcctcg
    16501 ccgatcggct ctcactgctg cagcaggccg aggtggacgc gtggctgctg gcgggcggct
    16561 tcgacggcac gcgcagcccg cgagcgctcg aggcggcggc gctggccgcc gccgcccgac
    16621 ggcccgacgg cggacccgtg gtgtgggccg gcgcggcgga gctgacggca gaggtcgaag
    16681 cgctgttcga gccgggggcg gtgacgagcg tcgccaatcc tcggccggac gggcgccggg
    16741 agacgccgga cgcgctgcgc gcccacctgc aggacctcat tgccgccgtc ggcggcggtg
    16801 agccggagac gaacctggcg tcgatgagct tcccacgggc cgttggcgcg ctcgcggcgc
    16861 gcagccggtt gcgggtgctg gccgtcgacg tgggtgcccg cgccgcggtc cgggcactgg
    16921 ccgagcggga cggcacggtg accagccggg tccacgccca gggcgggctc gcgggccttg
    16981 ccgcggttcc cggcgccgcc tcacgggtgg cccggctggc cggcgacgcg ggggacgagg
    17041 cggccgtggc cgacctgctc cagacgcttg cggcccgcgc ggcgacgctg cccgagacgg
    17101 ccgaggagct gtcggccatg cacgtcgctg cccggctcca ggcggcagcc ctgatcgagg
    17161 agggcggcgt cgggccgctg gacctgctca tcggcgcggg acggacgatc gccgccgcgc
    17221 cccaccccgc acaggcgttg cgcatcctgc tcgacggggt ccggccggtg ggcgtgacgc
    17281 agctggccct ggacgcggcg gcggccctcg gcccgctggg ctcgctgccg gacgcagaga
    17341 tcggggaggg gatcggtctg ctggctgacg acctgctggt cccgctcggc acgacggtcg
    17401 tcacgcgcgg cggcgatccc gggcgcctgg cgatgcgggt gacggtccac cgctcggggt
    17461 ggccggcctc cgcacccatc gagctgcgcg tcgggcagct gcaggtcgtg cccctgccgc
    17521 ccggccaggc cgcggagctg agcatcgagg tgggggacgg cgtgagcatc ggcgcaccgc
    17581 ggcggtcgcc gcggatcacc gctacggcca ccggcggtac cgttggcctg gtcctggacg
    17641 cgcgcggcgt gccggtgcag ctgccgcgtc gggccgacga ccgacgcgcg gtccttgccg
    17701 gctggcgcga cgccctcggg cgcgaaacgt cgtccaccgc ggagcgcctg gcatgagcgc
    17761 ggtccatcgc gtgcggctgc cggtcggcgc cacggtgttg gtggaggcgg gtcaccgcgt
    17821 cgagccggcg gaggtgctgg ccacccgacg accgccgggc gcgggcgtga cgatcccggt
    17881 ggccgtcccc ctccgccgtt cggccgcgga cgcgcgggag gcgctcgagg tcacgcccgg
    17941 atcggtcctg gaggccgatg cgctgcttgc ccgccagggc tcgcgcgagg ttcgcgtgcc
    18001 ggacgcctgc ctcttcctcg cctacgacgc cgatgacggg accgcgctga tcgcgcccct
    18061 cggcgcgacg gagccgatcc tcggccacgt gcgcggcgag gtcgcgagcg tcgcagcgga
    18121 cgccatcgag atccgggtcg acggggcgct ggtcaccggc atcgcgggga gcggcgccgc
    18181 ggtgcacggc gagctgcgcg tggcggtgca cgatcccggc gaggagctgc gcgccggcgc
    18241 catcgacgtg ggtgccacgg gccgcatcct ggtcggtggc tcccgggcgt cggcggagac
    18301 gctgacgcga gcgcgggcca tgggcgtcgc gggcatcgtg ctcgccggcg ccctggacaa
    18361 ggagctccgc gacttcgacg cagcccagcg gcgtcgccgg cagatcggcg gcgcgcgggg
    18421 cgcgttctcc atcctgctgc tcgagggcta cggcaagacg agcttcgacc cggccctctt
    18481 cgcctggttc cgcgcccacg acggacggat ggcgagcctc ttcggcgacg gcgcgcggct
    18541 ctatgtctac gatgccgagc ccgttcccgg gcgccgcgcg ctgccgaggc ccggcgaccg
    18601 ggtcatcgcg caccgccggc cgtcggccgg cgcgaccggc gtcctcgtgc gcgaggtccc
    18661 ggcgtcgtac gccgccgcgt ccggcgtggc agcccgctcg gcgctggtcc gcttcgacga
    18721 cgcgcgcacc gcaatcgtcc cgctggccaa cctggaggcg gccgagccgc ggtcggaggc
    18781 ctgaggccgc gcttcctata ctggcgcgca tggacccggc gaccctcacc ccgcacgcca
    18841 gcggcacccg ctcgacccgc gctcccgagg agacgcgggc actcggccgt gccatcggcc
    18901 aggcggcacc cgccgggacg gtgctggcgc tgatcggcga gctgggcgcg ggcaagacgc
    18961 agctcgcgaa gggcgtcgcc gaggggcttg gcgttcgcag cgtcgtcaac agcccgacct
    19021 tcgtgctgat gaacgagcat gccggccggc tgcggctgta ccacatcgac gcctatcggc
    19081 tgggcgatcc ggaggaggcc gtcgctgccg gcctgctcga cgagcgacag gcggatggcg
    19141 tggccgtggt cgagtgggcg gaccggctcg aggggtggct gccggacgat cggctcgaga
    19201 tcgcgctcga gggcgacggg gtcgatgccg accatcggcg gatcacctgg cgggccttcg
    19261 gcgcgcagca ccagcgcctg gccgcggagg cgctgcacgc gtgatcatcg ccatcgagag
    19321 cgcgtcgacc gacctgtcgc tcgcgctggc cgaacctgac ggcaccctga tcgccgacgc
    19381 ggcatggacg agccggcagc gccaatcggc cgagctgctc ccgcacctgc tccagctgct
    19441 cgatcagagc gggcaccgcc tcgaccaggc aaccgcgctg gcggtcggcc tcgggccggg
    19501 ctccttcacc ggcctgcgcg tggcgatgag cctgggcaag gggctcgccc tgtccctggg
    19561 tcgccccctg gtcggcgtgc cgagcctgga ggcgtggctg gcgtcggacg ctgcggccac
    19621 ggcggccgtg gcgcgcgcgg gggcgtccga ggcgtacgtg ctgcgtccgg ccgacgcggc
    19681 gccgcacatc gtggagcgcg atcgcctggc ggagatcatc ggcgaccatc cggtggtccc
    19741 cgacgagctg gccgccgcct tcgagctggg gagctccgcg tcgccgcgcc gcgccgcggc
    19801 ggccctcgcc cgcctcgcgg ccgaacggct ggccgcagcg cccgagggcg acgacctgcg
    19861 aaccatcgag ccgatctacc tgcgcgcgcc gcgaggcgtg cacgacaccc cgaacgaggg
    19921 ccgggtgacg tggctctgac cgcccgggtc gtcgtcgacc cgatgcgcct ggacgatgtt
    19981 cgggccgttc acgagatcga gcggctcagc ttcgccacgc catggccggc gtacgccttc
    20041 gagcaggagc tgaagggcaa ccggctggca cgttacgtcg tcgcgcgggc gggggagcgc
    20101 gttgtcggct tcgccggcat ctggctgatg gtggacgagg cgcacgtgac gaccttcggc
    20161 gtccaccccg actggcgtcg ccagggcatc ggccgccagc tgatgctcaa cgtcgccgag
    20221 ctgtcgctgg ccatcggcgc gcggcgcatg acgctggagg tgcgcgtctc caacgaggcg
    20281 gcccagggac tgtaccggtc gttcggcttc gaggtcgtcg gcaagcggcc gcactactac
    20341 accgacgatg gcgaggacgc gctggtgatg acgaccccgg cgctgcgcga gccgcacatg
    20401 cgccagatcg tcgccgcgca gcgccagctg atcggcacgt gagcgctccc cgcatcctcg
    20461 ccatcgaatc ctcgtgcgac gagaccggcg tcgcggtggt cgtcggtggg cgtcgcatcg
    20521 aggcgaacca ggtggcgacg caggtcgcgc tgca
//
LOCUS       DASTYP010000051         6956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1278512, whole genome shotgun sequence.
ACCESSION   DASTYP010000051 DASTYP010000000
VERSION     DASTYP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278512"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFV59_02645"
     CDS             <1..459
                     /locus_tag="VFV59_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5324759.1"
                     /translation="HFFSPVPVMKLIEIIRGLETDDATYQACKGFAEDVGKTVIESKD
                     MPGFLVNRMLGPFINEAVFALMESTGTAEDIDTGAKLGLNHPMGPLELSDFIGNDVML
                     NVMDVLYRGFGDPKFRAAPLLRQIVTAGHLGRKTGRGFYRYDEQGTKLPS"
     gene            456..1634
                     /locus_tag="VFV59_02650"
     CDS             456..1634
                     /locus_tag="VFV59_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5324760.1"
                     /translation="MTDAAATRDPLALTEEERAVRDTVRDWATKEVAPGAAERDEQER
                     YDRHLFEQAGELGLAGLPYPERYGGAGMGTFAWALAVEEIAAADMALAVSLSVHVLAQ
                     LCIVANGTDEQKDRFLPPMTAGRELGAFALTEPQAGSDAGSIALAARRDGDAYMLSGT
                     KIWITNAGEADRFVVFATVDRSRGKNGITAFVVDRETPGFRLGSKEKKMGIRGSSAHE
                     LVFEDARVPAENRLGEEGKGLRVALSALGAGRISIAAACVGLARNAMELAAAYALQRR
                     QFGRAISEQEMIQAMLAEMAVQVDAARLLTWRAARLRDAGQPINAPSSMAKWYASDVA
                     MKVTTDAVQVYGGAGYSRDNPVERLMRDAKGAQIYEGTNQIHRLIVAQQLLDTVGHDR
                     "
     gene            1652..3580
                     /locus_tag="VFV59_02655"
     CDS             1652..3580
                     /locus_tag="VFV59_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5324761.1"
                     /translation="MTPSPTQAPRKTISDPEPLVVPPEYQANNLIELVARAVDTYPNN
                     EAMRWKPTKTQRAEPGAEATDSGGWVHRTYREMWDWVTELSLGLRHLGLGDGDRFCII
                     SRTRPEWVIADFAGLALGAVSCPIYPQSEAGQAAFVINNVGAKIVVVENAQQAAKIAS
                     VRAQCPTLEHVITIDAHGKFPDGTLTYDDVMARADTDPAERRVWRETFHAIEPDHLAS
                     VIHTSGTTANPKGVMITHGGLVYQYQAARQLIDLHPTDVFLSWLPLAHSFEKITGEVL
                     PFAIGAAVAYAEPLIERLPANMVEVRPTVMAGVPRLFERVYGRVRSTIDTAPPLRQKI
                     FNWAIGLGAKRYANHLAGRKDSPWLAAQVKLADALVYKKVRERTGGRVRYFVSGAAPL
                     SREVGEFFYALGMLVLEGYGMTEASPLISVTRPADIVFGTVGQPAPGTQVKIDEFTGE
                     VCSRGPGTMVGYINEPEETAKAVDAEGWLHTGDVGELDEAGRLRITDRLKNIIVLANG
                     KNVSPGPMEAALSTSRLIGQAVILGDNQPYTGVLIAPDFDELGRWAAGNGLAEMPPEQ
                     LVEERSVQRLYDGEVKRLLDGFAIFERPRRVALLPRLLSEEEGELTPTLKTKLRVVQA
                     KWADKISYLFDEKGGGSD"
     gene            3610..3915
                     /locus_tag="VFV59_02660"
     CDS             3610..3915
                     /locus_tag="VFV59_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324762.1"
                     /translation="MTTASTTPTTDGAGLLVGETVHYRDHDRCWAAAVVGAAPDDAAQ
                     LYVFPLPPTVPMTANPGTFVGHDGGTTNETWHRLDECGTATDRPSRRTRRRRRTSER"
     gene            3965..5143
                     /locus_tag="VFV59_02665"
     CDS             3965..5143
                     /locus_tag="VFV59_02665"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU5324763.1"
                     /translation="MSDVFILSAARTPIGKFGGGLSTTPATQLGGIAIRAAVERAGIA
                     PERVDEVIMGQVIQAGAGQAPARQAALKAGLPDGVSATTINKVCGSGLKAVMLGASAI
                     RAGDADLIVAGGMENMNLGPYLLPEARTGYRLGNGSVVDSTVHDGLWCSSCDVHMGIH
                     AERVAAKHGVTREAQDEFALRSHQRAIAAQDEGRFDEEIVPVTVPGKKGDTVVSTDEA
                     PRSDTTLEALAKLRPAFQPDGGSVTAGNAPGITDGAAAVVIASGEALPDGAQPMARIT
                     GYAQADVAPEWLFEAPILGVRRLIEKVGGTLDDFDLIEINEAFAAQVLADGNELGFDW
                     DRVNVNGGAIALGHPIGASGARVLTTLLYELRRRGGGKGLATLCLGGGGAVAMAVETL
                     "
     gene            5151..5882
                     /locus_tag="VFV59_02670"
     CDS             5151..5882
                     /locus_tag="VFV59_02670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006377864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5324764.1"
                     /translation="MPDAIFDDPRLAAIYDALDPDRSDLDAYVAIVEEIGARSVLDLG
                     CGTGVFALMLADRGVDVIGVDPAGASLDVARAKAGAERVRWIHGDASAIPASVSVDAV
                     TMTANVAQVFLTDADWLATLAAVRRVLRAGGCLVFESRDPERRAWEEWTEERTRSTTQ
                     IPGVGAVTDWVEVTEVDEERGLVSFESPNVFEADGAVITARSTLRFRSREQLEASLRQ
                     SGFEVVEVRDAPDRPGRELVFLARR"
     gene            complement(5898..>6956)
                     /locus_tag="VFV59_02675"
     CDS             complement(5898..>6956)
                     /locus_tag="VFV59_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5324765.1"
                     /translation="TNTRYLHEHVVELAERITSSMPDGLDTVMFVNSGSEANDLAWRL
                     ATTVTGGDTGLVTDWAYHGVTHAIADFSPSEWPRGEQPAGVETVPAPNTYRGAFADAA
                     DPVAVAREQIAAAVQRMEARDRRPAALFIDSLFTADGIFVPDAEVVRAFVAGARDAGA
                     LFVADEVQSGHGRTGDDLWGFRAWGVTPDVVTLGKPMGNGHPIAAVVTRSDIVDRFAA
                     QTTFFSTFGGNPVACAAALAVLDVIEEGDLVARAAAVGRTLRAGIAALAERDHRVGDV
                     RGRGLTAGVELVTDRASRQPDAELAARVKEGMRERGVLIGTTGRTGNTLKIRPPLCIT
                     ADQAALIVETLAEVLGAA"
BASE COUNT         1065 a         2462 c         2482 g          947 t
ORIGIN      
        1 cacttcttct cgccggtgcc ggtcatgaag ctgatcgaga tcatccgcgg cctcgagacc
       61 gatgacgcga cgtaccaggc ctgcaagggc ttcgctgagg acgtcggcaa gaccgtcatc
      121 gagtcgaagg acatgccggg cttcctggtc aaccgcatgc tcgggccgtt catcaacgag
      181 gccgtcttcg cgctgatgga gtcgaccggc acggccgagg acatcgacac cggcgcgaag
      241 ctcggcctga atcacccgat gggcccgctg gagctgagcg acttcatcgg caacgacgtg
      301 atgctcaacg tcatggacgt gctgtaccgc ggcttcggcg acccgaagtt ccgcgcggca
      361 ccgctgctgc gccagatagt gaccgccggc cacctggggc gcaagacggg ccgcgggttc
      421 taccgctacg acgagcaggg caccaagctt ccgtcatgac cgatgccgct gcgacgcgcg
      481 accccctggc cctgaccgag gaggagcgcg ccgtccgcga caccgtccgc gactgggcga
      541 cgaaggaggt cgcgccgggt gccgccgagc gcgacgagca ggagcgctac gaccgccacc
      601 tcttcgagca ggccggtgag ctgggcctcg ccggcctccc gtatcccgag cgctacggcg
      661 gagctggcat gggcacgttc gcctgggcgt tggccgtcga ggagatcgcg gcggccgaca
      721 tggcgctcgc cgtcagcctc tcggtccacg tgctggccca gctgtgcatc gtggcgaacg
      781 ggacggacga gcagaaggac cggttcctcc cgccgatgac cgccggacgc gagctcggcg
      841 cgtttgccct gaccgagccg caggcggggt cggatgcggg cagcatcgcg ctggccgcgc
      901 gtcgggacgg ggacgcctac atgctcagcg gcacgaagat ctggatcacc aacgccggcg
      961 aggcggatcg gttcgtcgtc ttcgcgaccg tcgaccgatc tcgcggcaag aacggcatca
     1021 ccgccttcgt cgtcgaccgc gagacgcccg gattccgact ggggtcgaag gagaagaaga
     1081 tgggcatccg gggcagcagc gcccacgagc tcgtcttcga ggacgcgcgc gtcccggccg
     1141 agaaccggct cggcgaggag gggaaggggc tgcgggtggc gctcagcgcg ctgggcgccg
     1201 gtcgcatcag catcgcggcc gcctgcgtcg gcctcgcccg caacgccatg gagctggcgg
     1261 ccgcctacgc gctgcagcgt cgccagttcg gccgggccat ctcggagcag gagatgatcc
     1321 aggccatgct ggccgagatg gccgtgcagg tggacgcggc acggctgctg acgtggcggg
     1381 ccgctcgact gcgggacgct ggccagccca tcaacgcgcc gtcgagcatg gccaagtggt
     1441 acgcctcgga cgtcgccatg aaggtgacga cggacgccgt ccaggtgtac ggcggtgccg
     1501 ggtacagccg cgacaatccg gtcgagcgcc tcatgcgcga cgcgaagggg gcgcagatct
     1561 acgaggggac gaaccagatc caccggctga tcgtggccca gcagctgctg gacacggtcg
     1621 gccacgaccg atgacgacct gaggaggacc gatgacgccc agcccgaccc aggccccccg
     1681 caagacgatc agcgaccccg agccactcgt cgtcccgccc gagtaccagg cgaacaacct
     1741 gatcgagctg gtggcccgcg ccgtcgacac gtaccccaac aacgaggcga tgcgctggaa
     1801 gccaaccaag acgcagcgcg cggagcccgg cgctgaggcg accgacagcg gcggctgggt
     1861 gcatcgcacc taccgcgaga tgtgggactg ggtcacggag ctgtcgctcg gcctccggca
     1921 cctgggcctg ggtgacgggg atcggttctg catcatcagc cggacgcgtc cggaatgggt
     1981 catcgccgac ttcgccgggc tcgcgctcgg cgcggtctcg tgccccatct acccacagtc
     2041 ggaggcgggg caggcggcgt tcgtcatcaa caacgtcggc gcgaagatcg tggtggtcga
     2101 gaacgcgcag caggccgcca agatcgccag cgtccgcgcg cagtgcccga cgctcgaaca
     2161 cgtcatcacc atcgacgcgc acggcaagtt cccggacggg acgctgacct acgacgacgt
     2221 catggcgcgt gccgacacgg acccggcgga gcgtcgggtg tggcgcgaga ccttccacgc
     2281 catcgagccc gatcacctgg cgagcgtcat ccacacctcc ggcacgacgg ccaatccgaa
     2341 gggcgtgatg atcacgcacg gtggcctggt ctaccagtac caggccgcgc gccagctgat
     2401 cgacctgcac cccaccgacg tcttcctgtc ctggctgccg ctggcccaca gtttcgagaa
     2461 gatcaccggc gaggtgctcc ccttcgccat cggcgccgcg gtggcgtacg cggagccgtt
     2521 gatcgagcgc ctcccggcga acatggtcga ggtgcggccg accgtcatgg cgggcgtgcc
     2581 acggctgttc gagcgcgtgt acggccgcgt ccgatcgacc atcgacaccg cgccaccgct
     2641 gcggcagaag atcttcaact gggccatcgg actcggcgcc aagcgctacg ccaaccacct
     2701 ggccggccgg aaggactcgc cctggctggc cgcgcaggtg aagctggccg atgcgctcgt
     2761 ctacaagaag gtccgcgagc gcaccggggg acgcgtccgg tacttcgtct ccggcgccgc
     2821 gccgctctca cgggaggtcg gcgagttctt ctacgcgctg gggatgctgg tgctcgaggg
     2881 ctacggcatg accgaggcct ctccgctcat cagcgtcacg cggcccgcgg acatcgtgtt
     2941 cggcaccgtc ggccagcccg cgccggggac gcaggtgaag atcgacgagt tcaccgggga
     3001 ggtgtgctcg cgcggacctg gcacgatggt cgggtacatc aacgagcccg aggagaccgc
     3061 caaggcggtc gacgctgagg gctggctgca cacgggtgac gtgggcgagc tcgacgaggc
     3121 cggacggctg cgcatcaccg accggctcaa gaacatcatc gtcctggcca acggcaagaa
     3181 cgtcagcccc gggccgatgg aggcggcgct gtccaccagc cgcctcatcg gacaggccgt
     3241 catcctcggc gacaaccagc cgtacaccgg cgtgctgatc gcgcccgact tcgacgagct
     3301 cggccgctgg gcggccggga acggactggc cgagatgccg ccggagcagc tggtcgagga
     3361 gcgctcggtg cagcgcctgt acgacgggga ggtcaagcgc cttctcgacg ggttcgccat
     3421 cttcgagcga cctcgacgcg tggcgctcct gccacgcctc ctgtccgagg aggagggtga
     3481 gctcacgccg acgctgaaga cgaagctccg cgtcgtccag gccaagtggg ccgacaagat
     3541 ctcctatctc ttcgacgaaa agggcggcgg atccgactga cggcacggtt ggtgctgaca
     3601 gggaggccga tgaccactgc cagcaccacc cccaccaccg acggcgccgg gctgctcgtg
     3661 ggcgagacgg tccactaccg cgaccacgat cgctgctggg cggccgccgt cgtcggcgcc
     3721 gcaccggacg acgcggctca gctgtacgtc ttcccgctgc caccgaccgt cccgatgacc
     3781 gccaatcccg gcacgttcgt cggacacgac ggcgggacga cgaacgagac gtggcaccgc
     3841 ctcgacgagt gcgggacggc gacggaccga ccgtcccgcc gaacccgcag gcgacgccgc
     3901 acctcggaac gctgaccggg tctcgcgacg gggcatcggg cctcgccgcg gtaccatggc
     3961 gtccatgagc gacgtcttca tcctgtccgc cgcgcgaacg cccatcggca agttcggcgg
     4021 tggcctctcg accacgcccg cgacgcagct gggcggcatc gccatccgtg ccgcggtcga
     4081 gcgcgccggc atcgcgccgg agcgcgtcga cgaggtgatc atgggccagg tcatccaggc
     4141 cggagccggc caggcaccgg cgcgccaggc cgcgctgaag gccggcctgc ccgatggcgt
     4201 gagcgcgacc accatcaaca aggtgtgcgg atccgggttg aaggcggtga tgctcggcgc
     4261 atcggccatt cgcgcgggag atgcggatct catcgtcgcc ggcgggatgg agaacatgaa
     4321 cctcgggccg tacctgctgc ccgaggcccg cacggggtat cggctcggca acggcagcgt
     4381 ggtggattcg accgtgcacg acggcctgtg gtgctcgagc tgcgacgtcc acatgggcat
     4441 ccacgccgag cgcgtcgcgg cgaagcacgg cgtgacgcgc gaggcccagg acgagttcgc
     4501 gctgcgctcg catcagcgag ccatcgcggc acaggacgaa ggccgcttcg acgaggagat
     4561 cgtgccggtc accgtccccg gcaagaaggg ggacaccgtc gtctccacgg acgaggcgcc
     4621 ccgctccgac acgacgctgg aggcgctcgc caagctgcgc ccggcgttcc agccggatgg
     4681 gggcagcgtc acggccggca acgcgcccgg cattaccgac ggcgcggccg ccgtggtcat
     4741 cgcctccggg gaggcgcttc cggacggcgc gcaaccgatg gcgcgcatca ccggctacgc
     4801 ccaggccgac gtcgcccccg agtggctgtt cgaggcgccg atcctcggcg tgcggcggct
     4861 gatcgagaag gtcggcggca cgctcgacga cttcgacctg atcgagatca acgaggcgtt
     4921 cgccgcgcag gtcctggccg acggcaacga gctgggcttc gactgggatc gcgtcaacgt
     4981 gaacggcggc gccatcgcgc tcggccatcc catcggcgcg tccggcgccc gcgtcctgac
     5041 cacgctgctg tacgagctgc gccgccgcgg cggcggcaag ggcctggcga ccctgtgcct
     5101 gggcggtggg ggagcggtgg ccatggcggt cgagacgctc tagccgccgg gtgccggacg
     5161 ccatcttcga cgacccccgc ctggcggcga tctacgacgc gctggacccg gaccgctcgg
     5221 acctcgacgc gtacgtcgcc atcgtcgagg agatcggcgc tcgctccgtc ctcgatctgg
     5281 gatgcggcac gggcgtcttc gcgctgatgc tcgccgaccg cggtgtcgac gtcatcggcg
     5341 tggacccggc cggcgcatcg ctggacgtcg ctcgtgccaa ggccggcgcc gagcgcgtgc
     5401 ggtggatcca cggcgacgca tcggccattc cggcgtccgt ctcggtcgac gccgtcacga
     5461 tgaccgcaaa cgtcgcgcag gtcttcctca ccgatgcgga ctggctcgcc acgctggcgg
     5521 ccgtccgtcg ggtccttcga gccggcggct gcctcgtgtt cgagtctcgc gacccggagc
     5581 ggcgggcgtg ggaggagtgg acggaggagc ggacccgctc gacgacgcag atcccgggcg
     5641 tgggcgcggt gaccgactgg gtggaggtca ccgaggtcga cgaggagcgc gggctggtca
     5701 gcttcgagtc gcccaacgtc ttcgaggccg atggcgcggt catcaccgcg cgctcgaccc
     5761 tgcggttccg ttcccgcgag cagctcgagg cctcgctgcg ccagtcgggc ttcgaggtgg
     5821 tcgaggtccg tgacgcgccg gatcggcccg gccgggagct ggtcttcctg gcccggcgct
     5881 gagccgaccc cggccactca ggcggcgccc agcacctcgg ccagcgtctc gacgatcagc
     5941 gccgcctggt ctgcggtgat acacagcggc ggccggatct tcagggtatt gcccgtccgc
     6001 ccggtggtcc cgatcagcac ccctcgttcg cgcatcccct ccttgacgcg ggccgccagc
     6061 tcggcgtcgg gctgccgtga agctcggtcg gtgacgagct ccactccggc cgtgaggccg
     6121 cgccctcgta cgtcgccgac gcggtggtcc cgctccgcca gggcggcgat gcccgcgcgg
     6181 agcgtccgtc cgaccgcggc ggcgcgggcg accaggtccc cctcctcgat cacgtcgagg
     6241 acggcgagcg ccgcggcgca tgccacgggg ttgccgccga acgtgctgaa gaaggtggtc
     6301 tgcgcggcga accggtcgac gatgtcggag cgagtgacca ccgccgcgat cggatggccg
     6361 ttgcccatcg gcttgccgag ggtgacgacg tccggcgtca cgccccaggc acggaagccc
     6421 cagaggtcgt cgcccgtccg gccgtgaccg gactgcacct cgtccgcaac gaacagcgcc
     6481 ccggcgtccc gtgcgccggc gacgaaggcg cgcacgacct ccgcatccgg gacgaagatg
     6541 ccgtccgcgg tgaagaggga gtcgatgaag agcgccgcgg gccgccggtc tcgggcctcc
     6601 atgcgctgca cggcggccgc gatctgctca cgcgcgacgg cgacgggatc ggccgcgtcc
     6661 gcgaaggcac cgcgatacgt gttcggcgcg ggaaccgtct cgacgccggc cggctgctcg
     6721 ccgcgcggcc actccgatgg tgagaagtcg gcgatggcgt gcgtcacgcc gtggtacgcc
     6781 cagtcggtga ccaggccggt gtcgccgccg gtgaccgtcg tcgccagccg ccacgccagg
     6841 tcgttcgcct cgctcccgga gttgacgaac atcaccgtgt ccagcccgtc cggcatggac
     6901 gatgtgatcc gctccgccag ctcgacgacg tgctcgtgca ggtaccgcgt gttggt
//
LOCUS       DASTYP010000052         7501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1314496, whole genome shotgun sequence.
ACCESSION   DASTYP010000052 DASTYP010000000
VERSION     DASTYP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314496"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFV59_02680"
     CDS             <1..229
                     /locus_tag="VFV59_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324766.1"
                     /translation="YQEDERVRFQIAPLQNLPLPSVCGERVAPTPGPEPTPPPEATPP
                     PEATPPPPEGPPASPPGGGGGGGGGGGGGGG"
     gene            252..1520
                     /locus_tag="VFV59_02685"
     CDS             252..1520
                     /locus_tag="VFV59_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5324767.1"
                     /translation="MPGSVLTLILAGGAGERLSILSQVRAKPGVPFGGKYRIIDFALS
                     NTVNSGLTDVGILTQYAPRSLIDHIGVGRPWDLDRSRGGVALLQPYIGRGRSRDWYRG
                     TADAVLQNLEFIADRDPELVLVLAGDHVYKMDYRPFIALHRETGADATCAVRTVPIEE
                     AHRFGILDVATDGRVTAFIEKPANPPSNLVSMGVYVFSWPLLRDLLSPDRVDFGRDIL
                     PWMVDEGRRVFAYEFGGYWQDVGTVESYWATSLDLLSDDPGIELNDLGWLIYTRSEER
                     PPARIGADARIERSMVSHGCVVDGTVEHSVLSPGVRVAAGATVRDSIVMFDSVIEEGA
                     VLDRVIVDKEASIGPRARVGCGDDLRPNRDEPERLYAGITLVGKRARVPRGVEIGRNC
                     RIDPGTVERDFARRRVIRSGETVRAGFAAP"
     gene            1517..2089
                     /locus_tag="VFV59_02690"
     CDS             1517..2089
                     /locus_tag="VFV59_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324768.1"
                     /translation="MTGEAPPLDAWLAELGVEVVAAWSDPAGTAISRDVMVDGHRRFD
                     LRVTVAWVAGIGASLWAYYGLEAMEIPKRTFVRMLRANFDYPYVKFALTDDDRPMLMT
                     ELPTTALTRDELGRGLARMTVVADRLLEETALAVADRGVLPDWTGRTPRNPRLLATHR
                     PEVEAAMPVWEPPVPKRRRRGILSRLLGRA"
     gene            2090..4045
                     /locus_tag="VFV59_02695"
     CDS             2090..4045
                     /locus_tag="VFV59_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324769.1"
                     /translation="MRTRLSARMAVLLSVVAIVVAALPGRGVAPVRAADEYTMETVAA
                     YDVQPDAGVIDVSVQVSFTNTTPDPAGQFSVFDAVKLAIHDQATEVAATDGEGDLEVA
                     AAVESDVNVATVQLREDLRYEETATFTLTYTLPDSDDPHLRVRPSVVVFPAWSFGTSG
                     EVRVTIPDAYEVRVDGDPLTLDGDTLTSGDIPDPSNWLALVTGVRAADYTTLESAIPL
                     SGGTADLQVRAFSDDEAWGTATVDLVERALPLIEEEIGLPYPRLGQLVLTESVPADTT
                     GFAEAAPGGTEILVAYDQPPFTVIHQLVHVWVSPSFVESRWIREGLASSIAARVAEQL
                     GIERPYDPAAVTAERAGAAFPLDAWSANAGPDGEAYGYAASWDTVDQITGRVGVTALH
                     TVLARVAASIGPYQPADVEPQPDADGTTNPDAPLTTRAFLDHLETVAQTDLTDLFRGR
                     VLTETDTALLEARGAARGSFSALVTRAGSWGAPDPVRGAMTAWSFDDAQQQIEAAMAW
                     LEDRDAVRERMEAAGLGAPDRLQQAYRAHGGGPEAQAELDAERTVVDAYAETAADVNA
                     PRSFLERVGLLGGSDPANQLAMANGQFTDGDLQGAVDSIAEAQRILASAEGSGIVRLV
                     SAGLLVLILLAVAVILFRRRSSYTAAP"
     gene            4042..5082
                     /locus_tag="VFV59_02700"
     CDS             4042..5082
                     /locus_tag="VFV59_02700"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU5324770.1"
                     /translation="MTTEEAPARHVPHFPTAADVLSGETADVYFDRARTILAAEHLDP
                     VVTMEVFPRGEGVLCGAEEALTYLREVLADATDPEPVVETMHDGDTFRPKEIVMRITA
                     RYSAFGLYETAILGILASGSGWATAARRIVDAAAPIPVIGFGARHVHPSVADQLDYAS
                     VVGGCVGASTPAGARLAGLNPTGTMPHALVLIFGDTVRAAEAFDRHIPTDVPRIVLVD
                     TFKDEAEESLRVAEALGDRLWGVRLDTPSERGRVTPELVKEVRARLDLEGFQHVRIVV
                     SGGLDADRIGVFKDSGAPVDSFAVGSAISKASPIDFTGDLKEIDGKPVAKRGRIPGRT
                     ENPRLERIDLRA"
     gene            5092..5490
                     /locus_tag="VFV59_02705"
     CDS             5092..5490
                     /locus_tag="VFV59_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5324771.1"
                     /translation="MRQPSDRFDPPADRDSAYELFQRGSRMLADRHPGAAAVLLERAL
                     ALEPGKASILEALGQAYFNVGNHAAAAERFAAIVEADPIAHYAHFGLGLSSERLGDEV
                     AARRHLRMAVRLSPETEVYRQALSRVTRAA"
     gene            complement(5501..6646)
                     /locus_tag="VFV59_02710"
     CDS             complement(5501..6646)
                     /locus_tag="VFV59_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEU5324772.1"
                     /translation="MTTNTAPSPPEDALVPDPEESAEEAGLRYVTDTDGGVSRRRAGM
                     GWAFFDADGRRITDEKRIAWFKRLAIPPAWTDVWICPDKRGHLQATGRDARGRKVYRY
                     HPRWREVREDAKYGRMLAFARALPMIRKRVEEDLARPGLPREKVLAAVVALLERTRIR
                     VGNEEYARDNRSFGLTTLRDRHARIKGGRVTFQFKGKSGKEHAIELADRRLARIVERC
                     QDIPGQQLFQYVGDDGERRGIASDDVNAYLHEIAGERFTAKDFRTWAGTVLAAMALQE
                     FREFDSEAEAKKNVVRAIESVAEKLGNTPAVSRASYVHPQVIDAYLEGDLLREAREEA
                     DRKLTEDLDELTAEEAAVYALLRQRLSEEERRAERRERRQRRRPKAA"
     gene            complement(6679..7218)
                     /locus_tag="VFV59_02715"
     CDS             complement(6679..7218)
                     /locus_tag="VFV59_02715"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5324773.1"
                     /translation="MRLRTLSSERPWTGHRIGVRVDQVEAEDGRRTTREVVEHPGAVG
                     IIAWDGERLAMVRQWRHAPGEIVLEIPAGTLEPPEPPAETARRELAEEVALKAGVWET
                     GPRFWTAPGFCNELMHLYLATDLSDEPGFSDPDEDLEVEWLTVPEALAGIDDGSIRDA
                     KTVAAVLWLARRLERSAAT"
BASE COUNT          983 a         2713 c         2711 g         1094 t
ORIGIN      
        1 gtaccaggag gacgagcggg tccgcttcca gatcgcgcca ctccagaacc tgccgctgcc
       61 gtcggtgtgc ggggagcggg tggcgcccac accgggcccc gaacccactc caccgccgga
      121 ggcgacgcct cccccggagg cgacgccacc accgccggag ggtcctcccg cctcgccgcc
      181 gggcggtggc ggcggaggcg gtggcggcgg cgggggcggc ggcggctgag ccgccgacct
      241 agaatcggcc cgtgcccggc tccgtcctga ccctgatcct cgccggcggc gccggcgagc
      301 ggctgagcat cctctcgcag gtgcgcgcga agcccggcgt gccgttcggc ggcaagtacc
      361 ggatcatcga cttcgccctc agcaacacgg tcaactcggg cctcaccgac gtcgggatcc
      421 tgacccagta cgcgccccgc tcgctgatcg accacatcgg cgtcggacgg ccgtgggacc
      481 tggaccgcag ccgcgggggc gtcgcgctgc tgcagccgta catcggccgc ggccgatcgc
      541 gggactggta ccgcggaacc gccgacgcgg tgctgcagaa cctcgagttc atcgcggatc
      601 gagaccccga gctcgtgctc gtcctggccg gggatcacgt ctacaagatg gactaccgtc
      661 ccttcatcgc cctgcaccgc gagaccggcg ccgacgccac gtgcgcggtg cggacggtcc
      721 ccatcgagga ggcgcatcgg ttcggcatcc tggacgtggc gacggatggg cgggtcacgg
      781 cgttcatcga gaagccggcg aacccgccgt cgaacctggt gagcatgggc gtctacgtgt
      841 tcagctggcc actgctgcgc gacctgctca gccccgaccg cgtcgatttc gggcgggaca
      901 tcctgccgtg gatggtggac gaggggcgaa gggtcttcgc gtacgagttc ggcggctact
      961 ggcaggacgt tggcaccgtg gagagctact gggccaccag cctggacctg ctgagcgacg
     1021 acccgggcat cgagctgaac gacctgggct ggctcatcta cacccgcagc gaggagcggc
     1081 cgccggcccg catcggcgcg gatgcgcgga tcgagcgctc catggtcagc cacggctgtg
     1141 tcgtcgacgg cacggtcgag cacagcgtcc tctcacccgg cgtgcgcgtc gcggcaggcg
     1201 cgaccgtgcg cgactcgatc gtcatgttcg acagcgtcat cgaggaaggg gccgtgctgg
     1261 atcgggtcat cgtcgacaag gaggcgtcca tcggtccgcg cgcgcgcgtg ggctgcgggg
     1321 acgacctgcg gccgaaccgg gacgagccgg agcgcctcta cgccgggatc acgctggtcg
     1381 ggaagcgcgc gcgggtgccc cgcggggtcg agatcgggcg caactgccgg atcgatcccg
     1441 gcacggtgga gcgggacttc gcgcggcgcc gcgtgattcg ctccggggag acggtccgcg
     1501 ccgggttcgc cgccccgtga cgggcgaggc gcccccactc gacgcctggt tggcggagct
     1561 cggcgtcgag gtcgtcgccg cctggtccga cccggcgggc accgccatca gccgcgacgt
     1621 gatggtcgac gggcatcgac ggttcgacct gcgggtcacc gtggcgtggg tggccggaat
     1681 cggcgcctcc ctgtgggcat actatgggct ggaggcgatg gagatcccga agcgcacctt
     1741 cgtgcggatg ctccgcgcca atttcgacta cccgtacgtg aagttcgcgc tgaccgatga
     1801 cgaccggccg atgctgatga cggagctgcc cacgaccgcg ctgacgcgcg acgagctggg
     1861 gcgagggctg gcgcggatga cggtcgtggc cgatcggctg ctcgaggaga cggccctcgc
     1921 cgtcgccgac cgcggcgtcc tgcccgattg gaccggccgc acgccccgca accctcggct
     1981 gctggccacc caccgccccg aggtcgaggc ggccatgccg gtgtgggagc cgccggtccc
     2041 gaagcgacgg aggcggggga tcctgtcccg cctgctgggg cgcgcgtaga tgcggacccg
     2101 tctcagcgcg cgcatggcgg tgctcctgtc ggtggtcgcc atcgtggttg cggccctgcc
     2161 cggtcgcggc gtggcgccgg tgcgcgcggc cgacgagtac accatggaga cggtcgccgc
     2221 gtacgacgtc cagccggacg cgggcgtcat cgacgtctcg gtccaggtca gcttcacgaa
     2281 cacgaccccc gacccggcgg gtcagttctc ggtcttcgac gcggtgaagc tggccatcca
     2341 cgaccaggcg accgaggtgg cggccaccga tggcgagggc gatctcgagg tcgcggcggc
     2401 cgtggagtcc gacgtgaacg tcgcgacggt ccagctccgc gaggacctgc gctacgagga
     2461 gaccgccacc ttcacgctga cgtacacgtt gcccgatagc gacgatccgc acctgcgcgt
     2521 gcggccgtcg gtggtggtgt tcccggcgtg gagcttcggc accagcggcg aggtccgggt
     2581 gaccatcccg gacgcctacg aggtgcgcgt cgatggggat ccgctcacgc tcgacggcga
     2641 cacgctgacc agcggcgaca tcccggatcc gtcgaactgg ctcgcgctgg tgacgggcgt
     2701 gcgcgccgcc gactacacga cgctcgaatc ggccattccg ctgtcgggtg gcaccgccga
     2761 cctgcaggtc cgtgccttca gcgacgacga ggcgtggggc accgcgacgg tcgacctggt
     2821 cgagcgggcg ctgcccctga tcgaggagga gatcggcctc ccgtacccgc gcctcgggca
     2881 gctggtgctc acggagtccg tccccgcgga caccaccggc ttcgccgagg cggcccccgg
     2941 cgggaccgag atcctggtgg cctacgacca gccgccgttc accgtcatcc accagctcgt
     3001 gcacgtgtgg gtctcgccct cgttcgtgga gtcccgctgg atccgcgagg gcctcgccag
     3061 ctcgatcgcg gcgcgcgtcg cggagcagct cggcatcgag cgtccctatg accctgcggc
     3121 cgtcacagcc gaacgggccg gcgccgcgtt cccgctcgac gcctggtcgg ccaacgccgg
     3181 cccggacgga gaggcgtacg gctacgccgc ctcgtgggac acggtcgacc agatcaccgg
     3241 gcgcgtgggc gtgaccgcgc tgcacacggt gctggctcgc gtcgcggctt ccatcggtcc
     3301 ataccagccg gccgacgtcg agccccagcc ggacgccgac gggaccacca accccgatgc
     3361 gccgctgacg acgcgcgcgt tcctggacca cctcgagacg gttgcccaga ccgacctgac
     3421 cgatctcttc cgcgggcgcg tgttgaccga gacggatacg gcgctcctgg aagcgcgcgg
     3481 ggctgcacgc gggagcttct cggcgctggt gacgcgcgcc ggctcctggg gtgcccccga
     3541 cccggtccgt ggcgccatga cggcgtggag cttcgacgac gcgcagcagc agattgaggc
     3601 cgcgatggcg tggctcgagg atcgtgacgc ggtccgggag cgcatggagg cggccggcct
     3661 cggggccccc gaccgcttgc agcaggcgta ccgggcgcac ggcggcgggc cggaggcgca
     3721 ggccgagctc gacgccgagc gcacggtggt ggacgcctac gcggagactg ccgccgacgt
     3781 gaacgcgccg cgctccttcc tggagcgcgt gggtttgctg ggcggaagcg accccgcgaa
     3841 ccagctcgcc atggcgaacg gccagttcac cgatggcgac ctgcagggag ccgtcgactc
     3901 catcgcggag gcccagcgca tcctggcgtc ggcggagggc agcggcatcg tgcgtctcgt
     3961 gagtgccggg ctgctcgtcc tgatcctgct ggccgtggcc gtgatcctgt tccgccgccg
     4021 ctcgtcgtac actgctgcgc catgacgacc gaggaagcgc ctgcccggca cgtgccgcac
     4081 ttcccgacgg ccgccgacgt gctgtcgggc gagaccgccg acgtgtactt cgaccgtgcc
     4141 cggaccatcc tcgccgcgga gcacctcgac ccggtcgtca ccatggaggt cttcccgcgg
     4201 ggcgagggcg tcctgtgcgg cgccgaggag gcgctgacct atttgcggga ggtcctggcc
     4261 gacgcgacgg atccggagcc ggtggtcgag acgatgcacg acggcgacac cttccggccg
     4321 aaggagatcg tgatgcgcat cacggcccgc tactcggcct tcgggctgta cgagacggcc
     4381 atcctgggca tcctcgcctc cggctccggc tgggccacgg ccgcgcggcg gatcgtggat
     4441 gccgccgcgc ccatcccggt catcggattc ggagcgcggc acgtgcatcc gagcgtcgcc
     4501 gatcagctcg actacgcctc cgtggtgggc ggctgcgtcg gggcgtcgac cccggccggg
     4561 gcgcgactcg ccgggctcaa ccccacgggc accatgcccc acgccctggt cctgatcttc
     4621 ggtgacaccg tccgtgccgc cgaggcgttc gaccgccaca tcccgaccga cgtgccgcgc
     4681 atcgtgctgg tcgacacgtt caaggacgag gcggaggagt cgctccgcgt ggccgaggcc
     4741 ctcggtgacc ggctgtgggg cgtgcgcctg gacaccccat cggagcgcgg ccgagtcacc
     4801 cccgagctgg tcaaggaggt ccgcgcccgc ctggacctcg agggcttcca gcacgtccgc
     4861 atcgtcgtgt ccggcggtct cgacgccgac cgtatcggcg tcttcaagga cagcggcgcg
     4921 ccggtcgact cgttcgcggt gggcagcgcc atcagcaagg ccagccccat cgacttcacg
     4981 ggcgacctca aggagatcga cggcaagccg gtcgccaagc gcggccgtat cccgggccgg
     5041 accgagaacc cgcggctgga gcgaatcgac ctgcgggcgt aggctgtgcc gatgcgccag
     5101 ccctccgacc gcttcgaccc gcccgccgac cgcgattccg cctacgagct cttccagcgc
     5161 ggctcgcgca tgctggccga ccggcatccg ggcgccgcgg cggtgctgct ggagcgcgcg
     5221 ctggcgctgg aaccggggaa ggcgtccatc ctggaggcgc tcggacaggc ctacttcaac
     5281 gtcggcaatc acgccgcggc cgccgaacgg ttcgccgcga tcgtcgaggc cgatcccatc
     5341 gcgcactacg cgcacttcgg cctgggcctc tccagcgagc gcctgggcga cgaggtcgcc
     5401 gcgcgccgtc acctccggat ggcggtccgc ctgtcacccg agaccgaggt ctaccggcag
     5461 gcgctgagcc gggtgacgcg cgccgcctga cgcatcggtc ctaggcggcc ttcggccggc
     5521 gccgctggcg gcgctcccgc cgctccgcgc gtcgctcctc ctcgctcagc cgctgacgca
     5581 ggagggcgta cacggccgcc tcctcggccg tgagctcgtc gaggtcctcc gtcagcttgc
     5641 ggtcggcttc ctcgcgcgcc tcgcgcagca ggtcgccctc gaggtaggcg tcgatgacct
     5701 gtggatgcac gtagctggct cggctcacgg ccggggtgtt gcccagcttc tccgcgaccg
     5761 attcgatggc ccgcaccacg ttcttcttcg cctccgcctc ggagtcgaac tcgcggaact
     5821 cctggagcgc catggcggca agcaccgtcc cggcccacgt ccggaagtcc ttcgccgtga
     5881 agcgctcccc ggcgatctcg tgcaggtagg cgttgacgtc gtcgctcgcg atgccgcggc
     5941 gctcgccgtc atcgccgacg tactggaaca gctgctggcc cgggatgtcc tggcagcgct
     6001 cgacgatgcg cgccagccgg cggtcggcga gctcgatggc gtgctccttg cccgacttcc
     6061 ccttgaactg gaaggtcacc ctgccgccct tgatgcgcgc gtgccggtca cgcagcgtgg
     6121 tcaggccgaa gctgcggttg tcgcgggcgt actcctcgtt gccgacccgg atccgcgtgc
     6181 gctcgagcag ggcgacgacc gcggccagca ccttctcccg cggcagcccg ggcctggcga
     6241 ggtcctcctc gacgcgcttc cggatcatcg gcagcgcccg cgcgaaggcg agcatccggc
     6301 cgtacttggc gtcctcgcgg acctcgcgcc agcgcgggtg gtatcggtac accttgcggc
     6361 ccctggcgtc gcggccggtc gcctggaggt gcccgcgctt gtccgggcag atccagacgt
     6421 cggtccaggc cggcgggatg gcgagccgct tgaaccaggc gatccgcttc tcgtcggtga
     6481 tgcgtctccc gtcggcgtcg aagaaggccc agcccatgcc ggcacggcgt cggctgacgc
     6541 cgccgtcggt atcggtgacg tagcggaggc cggcctcctc agcggactcc tccgggtcgg
     6601 ggaccagggc gtcctcggga gggctcggtg cggtgttggt ggtcacggcg gcgctcaagg
     6661 caagccgcgt gccgagcgtc aggttgcggc gctgcgctcg agtcgccggg cgagccagag
     6721 gacggcggcg accgtcttcg cgtcgcggat gctgccgtcg tcgatgcccg ccagcgcctc
     6781 cggcacggtc agccactcga cctcgaggtc ctcgtcgggg tcgctgaagc ccggctcgtc
     6841 cgacaggtcg gtcgccaggt agaggtgcat cagctcgttg cagaagcccg gcgccgtcca
     6901 gaaccgcggg ccggtctccc acactccggc cttcagcgcg acctcctcgg ccagctcccg
     6961 acgggccgtc tccgccggtg gctccggcgg ctccagggtg ccggccggga tctcgagcac
     7021 gatctcgccc ggcgcgtggc gccactgccg caccatcgcc aggcgctcgc cgtcccacgc
     7081 gatgatgcca acggctcccg ggtgctcgac cacctcgcgc gtggtgcgcc ggccgtcttc
     7141 ggcctcgacc tggtcgacgc gcaccccgat gcggtggccc gtccacggcc gttcgctgct
     7201 gagtgttctg agtctcatgg cgccattgtg ctgggtgcgt accccgatcg gtgcgagtga
     7261 gccgctcagc agtctgctcg tcccccgaag ggtgcggacc ggtggctgag tggccaaggc
     7321 tggagtctgg tgggtgcgga aggacgttgc gtggctcata cgcatccgag cggggcaacg
     7381 cgcggcacgc cgggttcaga ggaggaccaa gccgttcgca ccggggtcga gcccggcact
     7441 gagcgccgca agcgttgttc gcgtatcaag gggaaagctc tgccgcaggg cgccgatgtg
     7501 a
//
LOCUS       DASTYP010000053         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_112748, whole genome shotgun sequence.
ACCESSION   DASTYP010000053 DASTYP010000000
VERSION     DASTYP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112748"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1383
                     /locus_tag="VFV59_02720"
     CDS             157..1383
                     /locus_tag="VFV59_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5324774.1"
                     /translation="MGLRIAVAGAGGFARDFLPLFAAHPGVEEVSLAELLPDRRAQVA
                     AEFGIRRTFASHEDACAAADVDAVAIMTQRHLHGPQAIATLEAGKHVWSAVPMGTDAT
                     EIGRILALAAEHRLVYMSAETSWYYPDVVFARARHAEGAFGEIVYAEGSYLHDMSQGF
                     YEAFQRSGGTDWRRVAGVPPMYYATHSLSSVLSVTGGRPVSVSCLGWADRSDDLVFGA
                     GRNLWDNPFSNQTALFRTSDGASVRINEFRRVGWSGRISELPMAIYGTRAVFAEHATG
                     QVWTDLDGHVEDLTERLAFPMDIGYAPAARPVDETDAPRRYAPVQRWQRLPRAFWSQR
                     NMHLGSHQFLVDDFVKAVTSGRLPPVSAWEAARFALAGITAHESALQDGERLQVPDPG
                     EPPADWARLDPEEATP"
     gene            complement(1394..1807)
                     /locus_tag="VFV59_02725"
     CDS             complement(1394..1807)
                     /locus_tag="VFV59_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324775.1"
                     /translation="MKRRSALLLGLLGCLSMILAACQSLPTPTATGTGTIDVRAVAGP
                     VCPVERQPPDPACAPRPVEGARILVQPGDGRDIVVGEATTDADGHGSVALPPGEYFVF
                     GLDVEGLMGRPEPVTVSVEPGETVTVDLVYDTGIR"
     gene            complement(1874..2512)
                     /locus_tag="VFV59_02730"
     CDS             complement(1874..2512)
                     /locus_tag="VFV59_02730"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU5324776.1"
                     /translation="MSDEKRAISVTGEGEASVAPDLALVSFAVTGNGRELGPVREEVL
                     TRSTAVLAALRDLGLADGDISAPDVSIQPEYDYRKGQRLVGYRVARNMTVRVRDLDRL
                     GPVLDGVVAAGANEVGGAQMTASDPSAAEHAALEAAMAAARTKAEVLAKAAGVALGRV
                     IRVEEEPTPGVGPMPKFRAMAAAAEYADASTEVVAGDLTVTRRIRAWFSIER"
     gene            complement(2525..2917)
                     /locus_tag="VFV59_02735"
     CDS             complement(2525..2917)
                     /locus_tag="VFV59_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324777.1"
                     /translation="MAVTDLSSGARLEQFGLRVRCADGDECNQRALEALMVALSRLGP
                     EVRNSPITSPGGAPAAAPVPPDRLYVEIWADPMLEWHSSTGAAGSASAFRVDLTNGNP
                     YVVVAPRWSFRLTEADAAAIRDALFVDA"
     gene            complement(3011..>4214)
                     /locus_tag="VFV59_02740"
     CDS             complement(3011..>4214)
                     /locus_tag="VFV59_02740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU5324778.1"
                     /translation="RTPVVVDGLRTPFGRYGGALKDIRPDDMAAHVIRALLDRTAVDP
                     SLIDDVILGAANQAGEDNRNVGRMAALLAGLPVDVPGQTVNRLCGSGLQAVVSAAHAI
                     AFGDGEVVVAGGVESMTRAPFVMAKPSAAFPRGEQTMHDTTLGWRFTNPRLADAHYPY
                     SMGETAENVVERCGVSREEQDAFAVASQQRWAHAHAAGRFADEVVPIEVPDGKGTRIV
                     DTDEHPRPDATLESLAGLKPAFKRDGSGSVTAGNSSGINDGAAALLVMSEARARELGL
                     RPLGRMVASAVAGVDPATMGLGPVPATRLALERAGLSVDDLDLVELNEAFAAQAVPVM
                     RELGLDPEKTNVNGGAIAIGHPLGASGARLAATLLHEMRRRGARYGLATMCIGVGQGI
                     AAVFEAVE"
BASE COUNT          608 a         1525 c         1501 g          580 t
ORIGIN      
        1 gagcacaccg cccagatcca gaagttcgtg gacatgggct tcgacgagat ctacctgcac
       61 aacgtcgggc gcgaccaggc ggcgttcatc gagaccttcg gcaccagggt gctgccgaac
      121 ctgaagctga gctgacggcc gtcggcggct gatccgatgg gcctcaggat cgccgtcgcc
      181 ggcgcgggtg gcttcgcccg cgatttcctg ccgctgttcg ccgcccaccc cggcgtggag
      241 gaggtctcgc tcgcggagct cctcccggac cgacgggcgc aggtggcggc cgaattcggc
      301 atccgccgca cgttcgcgtc gcacgaggac gcctgcgccg cggcggacgt cgacgcggtg
      361 gcgatcatga cccagcgcca cctgcacggt ccgcaggcca tcgcgaccct cgaggccggc
      421 aagcacgtct ggtcggccgt cccgatgggc accgatgcga ccgagatcgg ccgcatcctg
      481 gccctggcgg cggagcatcg gctggtctac atgagcgccg agaccagctg gtactacccc
      541 gacgtcgtct tcgcgcgtgc ccggcacgcc gagggcgcct tcggcgagat cgtgtacgcc
      601 gagggcagtt acctccacga catgagccag ggcttctacg aggccttcca gcggtccggc
      661 ggcacggatt ggcggcgcgt ggccggggtc ccgccgatgt actacgccac gcactcgctc
      721 agcagcgtcc tctcggtcac cggaggacgc ccggtcagcg tctcgtgcct tggctgggcc
      781 gaccggtcgg acgacctggt gttcggagcc gggcgcaacc tgtgggacaa cccgttcagc
      841 aatcagacgg cgctcttccg tacctcggac ggcgcgagcg tccgcatcaa cgaattccgc
      901 cgcgtgggct ggtcaggccg catcagcgag ctgccgatgg cgatctacgg cacccgcgcc
      961 gtcttcgccg agcacgccac cggccaggtg tggaccgatc tggacggtca cgtcgaggat
     1021 ctcacggagc gcctggcctt ccccatggac atcgggtatg ccccagccgc tcgacccgtt
     1081 gacgagaccg atgcgcctcg ccgctacgcg ccggtgcagc gctggcagcg cctgccccgg
     1141 gccttctggt cgcagcgcaa catgcacctc ggctcgcacc agttcctggt ggacgacttc
     1201 gtcaaggcgg tcacgagcgg acggctgccg cccgtcagcg cctgggaggc ggcgcggttc
     1261 gcgctggccg ggatcaccgc ccacgagtcg gcgctgcagg acggcgagcg gctgcaggtg
     1321 cccgatccgg gggagccgcc ggcagactgg gcgcgcctcg accctgagga ggcgacgccc
     1381 tgacgctcgg cgctcagcga atgccggtgt cgtagaccag gtcgacggtc accgtctccc
     1441 cgggctcgac gctgaccgtc acgggctcgg gtcgtcccat cagcccttcg acgtcgaggc
     1501 cgaacacgaa gtactcgcca ggcggcaacg cgaccgagcc gtggccgtcg gcatcggtcg
     1561 ttgcctcgcc gaccacgatg tcccggccgt ctccgggctg cacgaggatt cgcgcaccct
     1621 ccacggggcg cggcgcgcag gcggggtcgg gcggttgccg ctcgaccggg cacaccggcc
     1681 cggcgaccgc acgcacgtcg atcgtgccgg tcccggtcgc cgtcggagtc ggaagggact
     1741 ggcaggcagc aaggatcatc gacaggcagc cgaggaggcc gagcaggagg gccgaacggc
     1801 gcttcatgcg gtcagggacg cgggcgatcc gggttcggtt gctcgcccga tggcccggaa
     1861 gggcgtccgc gcgtcagcgc tcgatggaga accaggcgcg gatgcgacgc gtgacggtca
     1921 ggtcaccggc gacgacctcg gtcgacgcgt cggcgtactc cgccgcggca gccatcgcgc
     1981 ggaacttcgg catcgggccg acgccgggcg tcggctcctc ctcgacccgg atgacgcgtc
     2041 cgagcgccac gcccgccgcc ttcgccagca cctcggcctt ggtccgggcc gcggccatcg
     2101 ccgcctccag cgcggcatgc tcggctgccg acgggtcgga cgcggtcatc tgtgccccgc
     2161 ccacctcgtt ggcgccggcg gcgaccacgc cgtccagcac cggccccagg cggtcgaggt
     2221 cccggacccg caccgtcatg tttcgggcga cgcggtagcc gaccagccgc tgacccttgc
     2281 ggtagtcgta ctcgggctgg atcgacacgt cgggcgcgct gatgtcgccg tcggcgaggc
     2341 ccaggtcacg cagggccgcc agcaccgccg tggagcgcgt cagcacctcc tcccgcaccg
     2401 ggccgagctc gcgaccgttg ccggtgacgg caaaggacac gagcgccagg tccggagcga
     2461 ccgacgcctc tccctccccg gtgacactga tcgcacgctt ctcgtcagac attcgatttg
     2521 cctcctaggc gtcgacgaag agggcatcgc ggatggcggc ggcgtccgcc tccgtcaggc
     2581 gaaacgacca ccgaggcgcg acgacgacgt agggattccc gttggtcagg tcgacgcgaa
     2641 acgcggaggc gctgcccgcc gcgcccgtcg aggagtgcca ctcgagcatc gggtccgccc
     2701 agatctcgac gtagaggcgg tcgggcggta ccggtgcggc tgccggggcg ccaccgggac
     2761 tggtgatggg cgagttgcga acctccgggc cgagacgcga cagcgcgacc atgagggcct
     2821 cgagggcgcg ctggttgcac tcgtcgccat cggcgcagcg tacccgcagg ccgaactgct
     2881 cgaggcgagc gcccgaggag agatcggtca ctgccacgtt cgctaccgcc gtcgttccac
     2941 acgaggtggc gaggacggtg acgagacacg agatggtcaa tcccagggcg acgcatcgcg
     3001 ccatctcgac ctactcgact gcttcgaaga cggcggcgat gccctgccca acaccgatgc
     3061 acatggtcgc gaggccgtac cgggcgccac gacgccgcat ctcgtgcagc agcgtcgccg
     3121 ccaggcgggc gccggaggcg ccgaggggat ggccgatggc gatcgcaccg ccgttcacgt
     3181 tggtcttctc gggatccagg ccgagctcac gcatgacggg gacggcctgc gcggcgaagg
     3241 cctcgttgag ctccaccaga tccaggtcgt cgaccgagag gccagcacgc tcgagagcca
     3301 ggcgcgtggc ggggacgggg ccgagaccca tggtggccgg gtcgacgccg gccaccgccg
     3361 aggcgaccat gcggcccaac gggcgcaggc cgagctcacg cgctcgcgcc tcggacatca
     3421 ccagcagcgc ggccgcgccg tcgttgatgc cggacgagtt gccggccgtc accgatcccg
     3481 acccatcccg cttgaacgcc ggcttcagcc cggctaacga ctcgagggtg gcatcgggcc
     3541 gggggtgctc atcggtgtcg acgatgcgcg tgcccttgcc atcgggtacc tcgatcggga
     3601 ccacctcgtc cgcgaaccgt ccggcggcgt gcgcatgggc ccagcgctgc tgcgaggcca
     3661 cggcgaacgc gtcctgctcc tcgcgactga cgccgcagcg ctcgaccacg ttctcggccg
     3721 tctcgcccat cgagtagggg tagtgcgcat cggccaggcg cgggttcgtg aagcgccagc
     3781 cgagcgtcgt gtcgtgcatc gtctgctcgc cgcgcgggaa cgcggccgac ggcttggcca
     3841 tgacgaacgg cgcgcgggtc atggactcca cgccaccggc cacgacgacc tcgccgtcgc
     3901 cgaaggcgat ggcgtgtgcg gcgctcacca ccgcctgcag cccggagccg cacaggcggt
     3961 tgacggtctg cccgggaacg tcgactggca gcccggccag cagcgcggcc atgcgcccga
     4021 cgttgcggtt gtcctcgcct gcctggttgg ccgcgcccag gatgacgtcg tcgatcaacg
     4081 acgggtccac cgcggtccgg tcgagcaggg cgcggatgac gtgcgccgcc atgtcgtcgg
     4141 gtcggatgtc cttcagcgcg ccgccgtacc ggccgaaggg ggttcgcagg ccgtcgacga
     4201 cgaccggcgt gcgc
//
LOCUS       DASTYP010000054         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_404180, whole genome shotgun sequence.
ACCESSION   DASTYP010000054 DASTYP010000000
VERSION     DASTYP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404180"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..736
                     /locus_tag="VFV59_02745"
     CDS             59..736
                     /locus_tag="VFV59_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5324779.1"
                     /translation="MPFRTPMPLLIALVGVLVVILLGIGVATSATDPIDAPIVAAVRA
                     LPLAPLRVATEFGSWWGVTLVALLVIAVATLVESWRDGLAGALTIALASIGNTLLKAS
                     FHRARPDALDAIVVEHGYSFPSGHSALSMVAYGVLAVILWRSTLPRAARIGLVVALGL
                     LVFAVGLSRIWLGVHYPTDVLAGWTAGLAVVMVYAWLTRREPTEPAAATAVEGRAAPR
                     SDRPAVE"
     gene            complement(648..1214)
                     /locus_tag="VFV59_02750"
     CDS             complement(648..1214)
                     /locus_tag="VFV59_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324780.1"
                     /translation="MISHTTARSGRLSLGWLASGDRLADAIGRRLDDAYHWFRHVAPG
                     GIEPLEAVLVGPGGTWAISTAGERGRFTRRNGHWYRWNRSTESWVPWDAVPITAARLS
                     ARRLGLYLERASLPGAVQPLLVPPAEMAVLREPGEELGLTIEREPERLAALVSAEERL
                     TQPQVDRIVALLDPRQPLPRLAPSAPSG"
     gene            1328..1906
                     /gene="folE"
                     /locus_tag="VFV59_02755"
     CDS             1328..1906
                     /gene="folE"
                     /locus_tag="VFV59_02755"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU5324781.1"
                     /translation="MTDENGASLEKAVRTLLAEIGEDPGREGLVRTPERVRRMYDELT
                     AGYHIDPDALINGATFSVDYDEMVVVRDIEFFSLCEHHLLPFIGKAHVGYLPKGRVIG
                     LSKIPRIVDMYAQRLQVQERLTVQVADFLMERLEPKGVGCVIEATHLCTIMRGVKKQE
                     ATMVTSSMVGTFRRDARTRAEFMGLIGKGSAG"
     gene            1945..2358
                     /locus_tag="VFV59_02760"
     CDS             1945..2358
                     /locus_tag="VFV59_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5324782.1"
                     /translation="MTTDTPQTPIRVLIAKPGLDGHDRGAKVLARGLRDEGFEVIYTG
                     LRQTPEMIADAAEQEDVDVVGLSILSGAHMTLLPRTVQLLRERGMDDVLVAAGGIIPD
                     ADVVKLKDAGVAEVFGPGTSIGQIARYFREHVRRS"
     gene            2395..3297
                     /gene="meaB"
                     /locus_tag="VFV59_02765"
     CDS             2395..3297
                     /gene="meaB"
                     /locus_tag="VFV59_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU5324783.1"
                     /translation="MVDELASGGRRPLARLLTRIENGDPSVRALLPDLFAAGRGAHLV
                     GITGPPGSGKSTLVSALIGEWRRRGRRVGVLAVDPSSPYTRGAILGDRIRMMEHAADR
                     DVFMRSMASRGELGGLAATTWIAAAALDAAGFDPILVETVGAGQSEVEVARLAETTIV
                     VEVPEMGDEVQAIKAGLLEVADIIAVNKGDRPGADRAARQLRAMLSTAGGRIERRPPP
                     VLLTTATTAEGVAALADAVDAHREHARSPIAARDRAANQVRRALADLAARRAQDHHQW
                     DPIVRAVADRELDPMTAAERLLGE"
     gene            complement(3299..>3667)
                     /locus_tag="VFV59_02770"
     CDS             complement(3299..>3667)
                     /locus_tag="VFV59_02770"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5324784.1"
                     /translation="PGGAYSHVARVPAGATLVAIAGQISVAADGTFVGTGDFEAQCEQ
                     VYANIGAALEAAGGDWTNVMQMMTFLTRREDIARLRAWREREFPRFFPDGRYPPNTLL
                     VVSALANDDLLLEVQALAAI"
BASE COUNT          509 a         1275 c         1372 g          511 t
ORIGIN      
        1 gccggtcggg gagcgcatcg gctgcggatg atacgccccg cccctagact ggccgcccat
       61 gccattccgg acgccgatgc cgctgctcat cgcgctggtc ggcgtcctcg tcgtgatcct
      121 gctgggcatc ggcgtcgcca ccagcgccac cgacccgatc gacgcgccga tcgtggcggc
      181 cgtccgcgcg cttcccctgg ccccgctgcg ggtggcgacc gagttcggat cgtggtgggg
      241 cgtgacgctg gtcgctctgc tggtgatcgc ggtcgccacg ctggtcgagt cgtggcgcga
      301 cgggttggcg ggggcgctga ccatcgcgct tgcgtccatc ggcaacacgc tgttgaaggc
      361 ctcgttccac cgggcacgtc ccgacgccct ggacgcgatc gtggtcgagc acggctacag
      421 cttcccgtcc gggcactccg cgctcagcat ggtcgcgtac ggggtgctcg cggtgatcct
      481 gtggcgctcg acgctgccgc gcgcagcgcg catcggtctg gtcgtcgcgc tgggcctgct
      541 ggtcttcgcg gtcggcctgt cgcggatctg gctgggggtc cactacccga ccgacgtgct
      601 cgcggggtgg acggccggac tcgcggtggt gatggtctac gcgtggctca cccggaggga
      661 gccgacggag ccagccgcgg caacggctgt cgagggtcga gcagcgccac gatccgatcg
      721 acctgcggtt gagtgagccg ctcctcggca ctgacgagcg ccgccaggcg ttccggctcg
      781 cgctcgatgg tcaggcccag ctcctcgccg ggctcgcgga gcaccgccat ctccgcgggc
      841 ggcaccagca gtggctgcac cgcgccgggc agcgacgcgc gttcgaggta gagcccgagg
      901 cgacgcgcgg acagccgcgc ggcggtgatc gggacggcgt cccacggcac ccacgactcg
      961 gtggagcggt tccatcggta ccagtgcccg ttgcggcggg tgaagcggcc gcgctccccg
     1021 gcggtgctga tggcccacgt cccgccgggc cccaccagca cggcctcgag cggctcgatc
     1081 ccgccgggcg ccacgtgccg gaaccagtgg taggcgtcgt ccagccggcg gccgatcgcg
     1141 tcggccaggc ggtcgccgga ggccagccag ccgagggaga ggcgaccgga tcgggcggtg
     1201 gtatgggaga tcacggcagc cggaaccctc ctgacagctc gggtggtcgg atgggcatcc
     1261 ggagtgtacc gtccgcggcc ggcccggcgc cacgggtatc ggtcgggaag cccgtatcat
     1321 cgacgggatg accgatgaga acggggcctc cctggagaag gccgtccgca cgctgctggc
     1381 cgagatcggc gaggacccgg ggcgggaggg actggtccga acgcccgagc gcgtgcggcg
     1441 catgtacgac gagctgaccg ccggctacca catcgaccct gacgcgctga tcaacggcgc
     1501 gaccttcagc gtcgactacg acgagatggt cgtcgtgcgc gacatcgagt tcttcagcct
     1561 gtgcgagcac cacctgctgc cgttcatcgg caaggcgcac gtcggctacc tgccgaaggg
     1621 ccgggtcatc ggcctctcga agatcccgcg catcgtcgac atgtatgccc agcggctgca
     1681 ggtccaggag cggctcacgg tccaggtcgc cgacttcctg atggagcggc tggagcccaa
     1741 gggcgtgggt tgcgtgatcg aggcgacgca cctgtgcacg atcatgcgcg gcgtgaagaa
     1801 gcaggaggcg acgatggtca cctccagcat ggtcggaacg ttccgtcgcg acgccaggac
     1861 ccgcgccgag ttcatgggcc tgatcgggaa ggggagcgcc gggtagcgtc ggcggccgct
     1921 gcccgccggt agaatcggcg gcgcatgacg accgatacgc cccagacgcc gatccgcgtc
     1981 ctgatcgcca agcccggcct cgatggccac gatcgcggtg ccaaggtgct cgcccgcggc
     2041 ctgcgcgacg agggcttcga ggtgatctac accggcctgc gccagacgcc cgagatgatc
     2101 gcggacgccg ccgagcagga ggacgtcgac gtggtcggcc tctcgatcct gtccggagcc
     2161 cacatgaccc tcctgccacg cacggtgcag ctgctgcggg agcgcggcat ggacgacgtg
     2221 ctggtcgccg ccggcgggat cattcccgac gcggacgtgg tgaagctcaa ggacgcgggc
     2281 gtggccgagg tcttcggtcc cggcaccagc atcgggcaga tcgcgcggta cttccgcgag
     2341 cacgttcggc gctcgtgacc gcgccgcgta tatcgcgata tacgctggcg gccgatggtc
     2401 gatgagctcg cgtcaggagg ccgtcgtcca ctcgcgcgcc tcctgacccg catcgagaac
     2461 ggcgatccat cggtccgagc cctcctgccc gacctcttcg ccgcgggccg cggcgcgcat
     2521 ctggtcggca tcaccgggcc gccgggcagc ggcaagtcga cgctcgtcag cgcgctcatc
     2581 ggggagtggc gccggcgcgg acgccgggtc ggcgtgctgg ccgtggatcc cagctcgccg
     2641 tacacgcggg gcgccatcct gggcgatcgg attcggatga tggagcacgc ggccgaccgc
     2701 gacgtgttca tgcgctcgat ggcgtcgcgc ggtgagctcg gcggactcgc ggcgaccacg
     2761 tggatcgcgg cggcggcgct ggacgcggcg ggcttcgacc cgatcctggt cgagaccgtg
     2821 ggggcgggcc agtccgaggt ggaggtggcc cgactggcgg agacgacgat cgtggtcgag
     2881 gtgcccgaga tgggcgacga ggtgcaggcc atcaaggccg gcctcctcga ggtcgccgac
     2941 atcatcgccg tgaacaaggg cgaccggccc ggggccgatc gcgccgcgcg acagctgcgc
     3001 gcgatgctct ccacggcagg tggtcggatc gagcgccggc cgccgccggt cctgctgacg
     3061 acggcaacca ccgccgaagg ggtggccgcc ctggccgacg ccgtcgacgc ccaccgggag
     3121 cacgcgcggt cgccgatcgc ggcccgagac cgcgcggcca accaggtgcg ccgggcgctg
     3181 gcggacctcg cggcgcgccg tgcacaggat caccaccagt gggacccgat cgtccgggcg
     3241 gtggccgatc gagagctgga cccgatgacg gccgcggaac ggctcctggg ggagtgacct
     3301 agatcgcggc cagggcctgc acctcgagga gcaggtcgtc gttggccagc gcgctgacga
     3361 cgagcagcgt gttcggcggg tagcgcccgt ccgggaagaa gcgcgggaac tcgcgctcgc
     3421 gccaggcgcg caggcgagcg atgtcctcgc gacgggtgag gaaggtcatc atctgcatca
     3481 cgttggtcca gtcgccgccg gccgcctcca gcgccgcgcc gatgttcgcg tacacctgtt
     3541 cgcactgcgc ctcgaagtcg cccgttccca cgaacgtgcc atcggcggcc acggagatct
     3601 ggccggcgat ggcgaccagc gtcgcgccgg ccggaacgcg cgcgacgtgc gagtaggcgc
     3661 cgccggg
//
LOCUS       DASTYP010000055         7010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1096743, whole genome shotgun sequence.
ACCESSION   DASTYP010000055 DASTYP010000000
VERSION     DASTYP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096743"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFV59_02775"
     CDS             <1..483
                     /locus_tag="VFV59_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5324785.1"
                     /translation="SGRRLRGIRRGLGLADDGDASPDGSIRLHLLSAPVTFEGGQQAF
                     DAIRSVNPKPTAVLATSDVTAIGILRAATDAGNRVPHDLSIVGFDDIPAASWTTPRLT
                     TVHQPIREKGRRAAQRLIAAIRAGAEHQPAIEVLPTHLVVRDSTGPARGGGGEPASRG
                     "
     gene            528..1895
                     /locus_tag="VFV59_02780"
     CDS             528..1895
                     /locus_tag="VFV59_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5324786.1"
                     /translation="MSHRILRSMLPLGVILTLVLAACGGGGTSPSAGESTAESTAPSA
                     STAESQAPASQDATAACQDVDSLTFTHTMNDQESPVIKEIAEGFTGTTVEVTQIPFEG
                     AQQAYDQQAAGGEAPDVFRAEIAWIADYADKGYLVDLTDLIPQAEWDDFLPGPLAYGQ
                     WQGGTYALPQVTDALALLYNKSMFDEASVEVPTTMDEFVTAGQTLQEQLGLDNAFYMR
                     GDAYWLQPFLWAFGGGLFDVNDQGQVTDILVNNEGSVAGLEFLLNEVLGVIAPADVDF
                     ANDYGNAMTAFKEGDVAMIFNGPWATTDILSGTEFSDSENLGIAVIPAGPDGDKGSPI
                     GGHSYVMYAGMDEAKQECAAAFMLYLDSAENQARLASELNLLPTRTSAYELEDVASNP
                     LIADFQTVIENATARPVVPEGGQIYTDFGPHFQAAWTGDETPQEALDAVASAWETLLS
                     SRMAQ"
     gene            2003..2890
                     /locus_tag="VFV59_02785"
     CDS             2003..2890
                     /locus_tag="VFV59_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5324787.1"
                     /translation="MRERMWVAYAFLAPVALVMAVIVLYPLVGSVWSSFTDLNRSNAA
                     TVFRPSSEQFVGLQNYITILGSDEFRGRLVWTIVWTAVNVFFHFTLGLGLALLLNQKF
                     RGRTVYRLILMLPWAVPAFISAVSWRYIFNGEYGVLNLFLAGFGVDGPNWLSDPFWAY
                     VAPIIVNVWLGVPFMMVALLGGMQSIPSDLYEAARVDGANRRQQFWNVTLPQLKSVSV
                     AVTMLGIIWTFNLFVVIFLTTGGGPGGRTDILVTESFNLFQAGQYAAAATYSVIILSL
                     LLVFVTIYQRVFKVEEVTG"
     gene            2887..3735
                     /locus_tag="VFV59_02790"
     CDS             2887..3735
                     /locus_tag="VFV59_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5324788.1"
                     /translation="MKQPLWEKIATHAILIFACLIAIFPILWVISTSFKNQYDVLSTE
                     IHLIPVEPTLENYRSLFDPNSATGGYFWSWFGNSLLIAVLTTILSVFLASTAAYSLSR
                     YRFWGKAPITSFFLTSQMFPAVILLVPLFQLLAVQFRLLNTPWALVLAYATTAIPFCV
                     LMLKSYFDTLPIELEEAAKIDGLGPFGAFWQIVVPLSTPGIAVTAFYAFITAWNEFLY
                     ALVFLTKPEAFTLPIGVRLFINQFTQNWGGTMALSVIVTIPVMIFFYVAQRYLISGLS
                     TGGVKG"
     gene            complement(3732..3932)
                     /locus_tag="VFV59_02795"
     CDS             complement(3732..3932)
                     /locus_tag="VFV59_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324789.1"
                     /translation="MELDDRYDRDDVDPADALGGAGALPLPEGQPRGVVPTDRDAPQR
                     EAAPEAVDDLDQEPEPEPPADR"
     gene            3980..5500
                     /locus_tag="VFV59_02800"
     CDS             3980..5500
                     /locus_tag="VFV59_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5324790.1"
                     /translation="MGPLAELFETNRIIVLSIYGQVFFVLGLAVALQSRRRSELSLAR
                     SLGWLAAFGLLHGLMEWGYLFIPIQAGFLPRPIIEALLVLQLAMKPVSFALLFQFGAE
                     LLIAIGVPSGGPRPFGQPWPRLVPAATLFAWLLLTVLLSVAVPDAFAPAPGPWLPSDE
                     LAAAFADVGAPLVVGDVVARLLIGLPGALLVAYGLRVTARRIEPVGGPRVVSALRVAA
                     IAFAAYAVVAGLIGLPAPFPPASILNDRLVLATLGVPIEVFRSLAGLAVALAVIRSLE
                     LFELETDRALAEARRRELLLRERERIGRDLHDGIIQSIYAAGLHLEQASTEIGEGERA
                     RERIGTVMAELNRITNDIRSTIFDLRSGELESRDAEAIVLAVADELQAHTLVGLEISM
                     EGLFRPRLRPEQAEHLRHLVTEAFSNILRHARATNVAVRMACTRRRFTLEIQDDGTGF
                     DPGADAGRGRRGRAQGVANMRRRAELLEGDLSIESEPGRGTLLSLTMPVATVRRPT"
     gene            5516..6205
                     /locus_tag="VFV59_02805"
     CDS             5516..6205
                     /locus_tag="VFV59_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5324791.1"
                     /translation="MTTAPTPPASPIRILIADDHEVVRIGLASLLEAQEGFTVVAQAE
                     SGAEAVRLTRRFRPDVVVMDIRMPGGSGVEACRTITTEMEDIPVIMLTSHADSDALFD
                     AIDAGASGYVLKRVGSGDLIEAVRTVAAGGSMLDPAITKPVLDRLRNASRLEEAGAFS
                     DLTEQERRVLANVADGASNREIAERMGLAEKTVRNYVSSILAKLGLDSRAQAAGYAIR
                     NRLPELDEDRS"
     gene            6202..6858
                     /locus_tag="VFV59_02810"
     CDS             6202..6858
                     /locus_tag="VFV59_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324792.1"
                     /translation="MTFARADRRTILPLVAAVLVAGCVAQPTPTPVPTASATPSLTPS
                     ASAAPTETPTVSAAPSETPEPSLSLDLPDGRDDRIVAVSVRVDVPPDADGTITVAVTS
                     RVEAMIDELVLRWPTELDATLFLAPFTPSDERIRDGGPPLVQPWTKWVVGPGEQGEPD
                     GTTSLGWGPLPGNGTLTIPIVVNRVAPGPVAFDLQVLARNDLLALEDGSPAELRVEVP
                     "
BASE COUNT         1000 a         2562 c         2312 g         1136 t
ORIGIN      
        1 tccggccgcc ggctgcgcgg cattcgccgc ggcctgggcc tcgccgatga cggtgacgcg
       61 tcaccggacg gcagcattcg cctccatctc ctgtccgctc ccgtgacctt cgagggcggg
      121 cagcaggcct tcgacgccat caggagcgtc aacccgaagc cgacggcggt cctcgcgaca
      181 tccgacgtca ccgccatcgg catcctgcgc gcggcgaccg acgccggcaa ccgcgtgccg
      241 cacgacctgt ccatcgtcgg cttcgacgac atccccgcgg cgtcgtggac gacgccgcgg
      301 ctgaccacgg ttcaccagcc gatccgcgag aagggtcgcc gtgcggccca acgcctgatc
      361 gccgcgatcc gggccggtgc ggagcaccaa cccgccatcg aggtcctgcc gacccacctg
      421 gtcgtgcggg actcgaccgg accggcgcga gggggaggag gtgagcccgc gagccgagga
      481 tgaccctgcg ccaccacgat caccacggaa tggagagagc tgaacccatg agtcaccgaa
      541 tccttcgcag catgctgccg ctcggcgtga tcctcacgct cgtgctggcc gcttgcggcg
      601 gcggcggcac ttcgccgagc gccggcgaga gcacggccga gagcaccgcg ccgtcggcga
      661 gcacggccga gagccaggcg ccggcgtcgc aggacgcgac cgccgcctgc caggacgtcg
      721 attccctcac cttcacccac accatgaacg accaggagtc gccggtgatc aaggagatcg
      781 ccgagggctt cacgggcacg accgtcgagg tcacccagat tccgttcgag ggcgcgcagc
      841 aggcctacga ccagcaggcc gccggcggcg aggcaccgga cgtgttccgc gccgagatcg
      901 cctggatcgc cgactacgcc gacaagggct acctggtcga cctgacggac ctgatcccgc
      961 aggccgagtg ggatgacttc ctccccggcc cgctcgccta cggccagtgg cagggcggca
     1021 cctacgcgct gccgcaggtc accgacgcgc tggcgctcct ctacaacaag tcgatgttcg
     1081 acgaggccag cgtcgaggtc ccgaccacca tggacgagtt cgtgaccgcg gggcagacgc
     1141 tccaggagca gctcggcctg gacaacgcgt tctacatgcg cggtgatgcc tactggctgc
     1201 agccgttcct gtgggcgttc ggcggcggcc tgttcgacgt gaacgaccag ggccaggtca
     1261 cggacatcct cgtcaacaac gaggggtcgg tcgctggtct cgagttcctc ctcaacgagg
     1321 tcctcggcgt catcgctccg gcggacgtcg acttcgccaa cgactacggc aacgccatga
     1381 ccgccttcaa ggagggcgac gtggcgatga tcttcaacgg cccctgggcc accaccgaca
     1441 tcctgtccgg caccgagttc agcgattccg agaacctcgg catcgcggtc atcccggccg
     1501 gcccggatgg cgacaagggc tcgccgatcg gcggccacag ctacgtcatg tacgcgggca
     1561 tggacgaggc gaagcaggaa tgcgccgccg ccttcatgct ctacctggat tcggccgaga
     1621 accaggcacg cctggccagt gagctgaacc tgctcccgac ccgcacgagc gcctacgagc
     1681 tcgaggacgt cgcgagcaac ccgctcatcg ccgacttcca gacggtcatc gagaacgcca
     1741 ccgcccggcc cgtcgtgccg gagggtggcc agatctacac cgacttcggt ccgcacttcc
     1801 aggcggcctg gaccggtgac gagaccccgc aggaggcgct cgacgccgtc gcatcggcct
     1861 gggagaccct tctctccagc cggatggcgc agtagagtcg ccgtcatcag gcctcgggcc
     1921 ggtcgctcag gcggccggct cggggcacgt caacaggccc gaccgcacct gagcaactcg
     1981 acgttcgagg agacgcaagc cgatgcgtga acggatgtgg gtggcctacg ccttcctggc
     2041 gccggtcgcg ctcgtcatgg ccgtcatcgt tctgtatccg ctggtgggat cggtgtggtc
     2101 cagcttcacc gacctgaacc gcagcaacgc cgcgaccgtc tttcggccga gctccgagca
     2161 gttcgtcggc ctgcagaact acatcaccat cctgggcagc gatgagtttc gcggacggtt
     2221 ggtgtggacc atcgtctgga ccgcggtcaa tgtcttcttc cacttcaccc tgggcttggg
     2281 gctggcgctc ctgctcaacc agaagttccg cggtcgcacc gtctaccggc tcatcctcat
     2341 gctgccgtgg gccgtgccgg ccttcatcag cgcggtgagc tggcgctaca tcttcaacgg
     2401 cgagtacggc gtcctgaacc tcttcctcgc cggcttcggg gtcgacggcc cgaactggct
     2461 gtccgatccg ttctgggcct acgtcgcacc catcatcgtc aacgtctggc tcggcgtgcc
     2521 gttcatgatg gtcgccctcc tgggcggcat gcagtcgatc ccatccgacc tctacgaggc
     2581 ggcccgcgtc gacggcgcca accggcggca gcagttctgg aacgtgacgc tgccgcagct
     2641 gaagtccgtt tccgtcgcgg tgaccatgct cggcatcatc tggaccttca acctgttcgt
     2701 cgtcatcttc ctgacgaccg gcggcgggcc gggcggtcgc accgacatcc tggtcaccga
     2761 gtcgttcaac ctgttccagg ccggccagta cgctgccgcg gcgacctact cggtcatcat
     2821 cctgtccctg ctgctcgtgt tcgtcacgat ctaccagcgc gtcttcaagg ttgaggaggt
     2881 cacggggtga agcagccgct gtgggagaag atcgccaccc acgcgatcct catcttcgcc
     2941 tgcctgatcg cgatcttccc gatcctgtgg gtcatctcca cctccttcaa gaaccagtac
     3001 gacgtgctct cgaccgagat ccacctcatc ccggtcgagc cgaccctcga gaactatcgc
     3061 tcgctgttcg atcccaacag cgcgacgggc ggctacttct ggagctggtt cggcaactcg
     3121 ctgctcatcg cggtgctgac cacgatcctg tcggtcttcc tggcctcgac ggccgcctac
     3181 tcgctgagcc gataccggtt ctgggggaag gcgccgatca cgagcttctt cctgaccagc
     3241 cagatgttcc cggcggtcat cctgctggtc ccgctgttcc agctgctggc cgtgcagttc
     3301 cgactgctga acacgccatg ggcgctcgtc ctggcctatg cgacgacggc gatccccttc
     3361 tgcgtgctga tgctgaagag ctacttcgac acgctcccga tcgagctcga ggaggcggcg
     3421 aagatcgatg gcctcggccc gttcggtgcc ttctggcaga tcgtcgtgcc gctttcgacg
     3481 ccgggcatcg cggtgacggc gttctacgcc ttcatcaccg cgtggaacga gttcctctac
     3541 gccctggtct tcctcaccaa gccggaggcg ttcaccctgc cgatcggcgt gcggctgttc
     3601 atcaaccagt tcacgcagaa ctggggtggc acgatggccc tctcggtcat cgtcaccatc
     3661 ccagtcatga tcttcttcta cgtcgcccag cgctacctga tcagcggcct ctcgacggga
     3721 ggcgtgaagg gctagcggtc ggcgggcggc tccggctccg gctcttggtc gagatcatcg
     3781 accgcctccg gcgccgcctc gcgctgcggg gcgtctcgat cggtgggcac caccccgcgt
     3841 ggctgtccct cgggcagcgg cagcgcgccg gcgccgccga gcgcgtcggc ggggtcgacg
     3901 tcgtcgcggt cgtatcggtc atcgagttcc atgcgggcag aggcatgcaa gcgccgggcc
     3961 gccccgtacg atggtgagca tgggtccgct ggccgagctg ttcgagacaa accggatcat
     4021 cgtcctgtcc atctacgggc aggtcttctt tgtgctcggc ctggccgtcg cgctccagag
     4081 ccgccgccga tccgagctgt cgctcgcccg ctcgctcggc tggctggccg ccttcggcct
     4141 gctccacggc ctgatggagt ggggctacct cttcatcccg atccaggccg gcttcctgcc
     4201 acgaccgatc atcgaggcgc tgctggtcct gcagctggcg atgaagccgg tgagcttcgc
     4261 gctcctcttc cagttcggcg ccgagctgct catcgccatc ggcgtgccga gcggtggccc
     4321 ccgaccgttc ggccagccgt ggccgcgcct ggtgccggcc gcgacgctgt tcgcctggct
     4381 gctgctcacc gtgctgctct cggtcgccgt ccccgatgcc ttcgctccgg cgcccggtcc
     4441 ctggctgccc tcggacgagc tcgcggccgc gttcgccgac gtcggcgccc cgctggtggt
     4501 cggcgacgtc gtcgcgcgcc tgctcatcgg cctgccgggg gcgctgctgg tcgcgtacgg
     4561 cctgcgggtc accgcccggc gcatcgagcc ggtcggcggg ccgcgcgtcg tgtcggcgct
     4621 gcgggtggcc gccatcgcct tcgccgcgta cgcggtggtg gccgggttga tcggcctgcc
     4681 ggcgccattc ccgcccgcct cgatcctcaa cgaccggctg gtgctcgcca cgctcggcgt
     4741 gcccatcgag gtcttccgtt cgcttgccgg gttggcggtg gcgctggccg tcatccgcag
     4801 cctggagctg ttcgagctgg agacggatcg cgcgctcgcc gaggcccgtc gccgcgagct
     4861 gctgctgcgg gagcgcgagc ggatcgggcg cgacctgcac gacggcatca tccagtcgat
     4921 ctacgccgcg gggctgcacc tggaacaggc gtcgaccgag atcggcgagg gggagcgggc
     4981 gcgcgagcgc atcggcaccg tcatggccga gctgaaccgc atcaccaacg acatccggag
     5041 caccatcttc gacctgcgct ccggcgagct cgagtcgcgc gacgcggagg ccatcgtcct
     5101 ggccgttgcc gacgagctcc aggcgcacac cctggtcggt ctcgagatca gcatggaggg
     5161 gctcttccgt ccccggctgc gtcccgagca ggccgagcac ctgcgccacc tggtgacgga
     5221 ggccttcagc aacatcctgc gccatgcccg tgccacgaac gtcgccgtgc ggatggcgtg
     5281 cacgcgacgg cgcttcacgc tcgagatcca ggacgacggc accggcttcg acccgggggc
     5341 cgatgccggc cgcggccgcc gcggccgggc ccagggcgtg gcgaacatgc ggcgtcgtgc
     5401 cgagcttctg gagggagacc tgtccatcga gagcgagccc gggcgcggga ccttgctctc
     5461 gcttacgatg ccggtagcga ccgtccgccg gcccacctga gcaacgacga gaacgatgac
     5521 caccgcgccg acaccgccag ccagcccgat ccgcatcctg atcgcagacg accacgaggt
     5581 cgtccgcatc ggtctcgcct cgctgctcga ggcccaggaa gggttcacgg tggtggcgca
     5641 ggccgagtcc ggtgccgagg cggtgcggct gacgcgccgg ttccgtcccg acgtcgtggt
     5701 gatggacatc cggatgcccg gcggctctgg cgtcgaggcg tgtcgcacca tcacgaccga
     5761 gatggaggac atcccggtca tcatgctgac cagccacgcc gactccgatg cgctgttcga
     5821 cgccatcgac gcgggagcgt cgggctacgt cctcaagcgc gtcggctcgg gcgacctcat
     5881 cgaggcggtc cgcaccgtgg ccgccggcgg ctccatgctc gatcccgcca tcaccaagcc
     5941 ggtgctcgac cggctccgca acgccagccg tctcgaggag gccggtgcct tcagcgacct
     6001 gaccgagcag gagcgccgcg tgctggcgaa cgtcgccgat ggcgcctcca accgggagat
     6061 cgccgagcgc atgggcctcg cggagaagac ggtgcgcaac tacgtctcca gcatcctcgc
     6121 caagctcggg ctcgacagcc gcgcccaggc ggccggatac gccatccgga accgcctccc
     6181 cgagctcgac gaggaccgtt cgtgaccttc gcccgcgcag accggcggac catcctgccg
     6241 ctcgtggccg cggtcctggt ggccgggtgc gtcgcgcagc cgacacccac gcccgtgccg
     6301 accgcatcgg ccacgccgag cctcacgccg tccgcgtcgg ccgcgccgac cgaaaccccc
     6361 acggtctccg ccgcgccttc cgagaccccc gaaccctccc tctcgctgga cctgcccgac
     6421 ggtcgcgacg accggatcgt ggcggtctcc gtccgggtcg acgtcccgcc ggacgccgac
     6481 ggcaccatca ccgtggcggt gaccagccgc gtggaggcga tgatcgacga gctggtgctg
     6541 cgctggccga ccgagctcga cgccacgctg ttcctcgctc cgttcacgcc gtccgacgag
     6601 cggatccgcg acggcggacc gccgctggtg cagccctgga cgaagtgggt ggtcgggccg
     6661 ggtgagcagg gggagccgga cggcacgacg tcgctgggtt ggggtccgct gccggggaac
     6721 ggaaccctca ccatcccgat cgtcgtgaac cgcgtcgcgc ccggtcctgt cgcgttcgac
     6781 ctccaggtcc tggcccgcaa cgatctgctg gcgctggagg atggctcgcc ggcggagctg
     6841 cgggtcgagg tgccctaggc gtgcgaccca tccttccggc ctggtccgat gccgacgccg
     6901 atctccacgc ccggctgggc gacgcgatcg accccgatcg caaggtgctc gccgcgctgg
     6961 agcggatcat gccgctcagc gggaagcgca tcgccgacgt cgggaccggc
//
LOCUS       DASTYP010000056         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1242374, whole genome shotgun sequence.
ACCESSION   DASTYP010000056 DASTYP010000000
VERSION     DASTYP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242374"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..500)
                     /locus_tag="VFV59_02815"
     CDS             complement(204..500)
                     /locus_tag="VFV59_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324793.1"
                     /translation="MTDQPPGDRNADLEARIAELERRSSWRSTVDEGRSGMETAFWAM
                     VRTLFPEDARKHLKAATREQLLAARVYLDKWIERLDREEAEAVAGRPHERIDIE"
     gene            complement(515..1693)
                     /locus_tag="VFV59_02820"
     CDS             complement(515..1693)
                     /locus_tag="VFV59_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEU5324794.1"
                     /translation="MSARILVYTGKGGVGKTSVAAATALACADRGYRTIVMSTDIAHS
                     LGDAFNVELGPTPKEIAPNLWAQESDVFFNVARYWGKIQAYAASVLRWRGLDEVMAEE
                     MTVLPGMDEVGSLLWIADHHDSGNYDVVVVDAAPTGETLRLLSLPEAARWWMEKIEPI
                     GRKITKLTGPLIQRVVGMPMPGDEVFNAGEELFGRLEHMHDLLTDHTKTSVRVVLTLE
                     QVVIKEAQRSFTYFHLYNYPTDLVVANRVLPDDQDWHYFRDWYEAQQRYGPLVEKTFH
                     PIPVRQAPYFPNEMVGIPLLRRLAAELYDGDDPTTFFYRGRPYEVARDADGFLLAVDL
                     PFTEKEQIDLSRHGDELVIDLGTWRRTLVLPRILVDAPTQGARFDNGTLKIRFGAPAR
                     "
     gene            1864..>2058
                     /locus_tag="VFV59_02825"
     CDS             1864..>2058
                     /locus_tag="VFV59_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEU5324795.1"
                     /translation="MRRFLLGLVATLVVAFVVIQLVPYGRAHDNPPVASEPNWDSPRT
                     REMAVTACFDCHSNETSWPWY"
BASE COUNT          292 a          689 c          750 g          327 t
ORIGIN      
        1 accaggatgc cgcgcttggt gcgctgcagc cgcgactcgg cggcggcgcg gcgaggatca
       61 gcgggtgcgg gacgagacga ggcgaacgac ggcatgccac cacggtgcgc cgccaggatg
      121 ggaatcgcat gggagcgcca tcggcgcgcc cctcgcgcga tgcgtgcggt gcgaccggcg
      181 ctcggcggcg ccggcgcatc ggtctactcg atgtcgatgc gctcgtgcgg ccgaccggcg
      241 accgcctcgg cctcctcgcg gtccaggcgc tcgatccact tgtcgaggta cacccgggcg
      301 gccaggagct gctcgcgcgt ggcggccttc aggtgcttgc gcgcgtcctc cgggaagagc
      361 gtgcggacca tggcccagaa ggcggtctcc atgccggagc gaccctcgtc cacggtcgag
      421 cgccacgagg agcggcgctc gagctccgcg atgcgggctt ccaggtcagc gttgcggtcg
      481 ccggggggct gatcggtcat cggttcatct cctgtcaacg ggccggggcg ccgaagcgaa
      541 tcttgagggt tccgttgtcg aagcgcgcgc cctgcgtggg cgcgtcgacc aggatgcgag
      601 gcaggaccag cgtgcggcgc cacgttccga gatcgatcac gagctcgtcg ccatggcgcg
      661 acaggtcgat ctgctccttc tcggtgaacg gcaggtcgac ggccagcagg aatccgtcgg
      721 cgtcgcgggc cacctcgtac gggcggccgc ggtagaagaa ggtcgtcggg tcgtcaccgt
      781 cgtacagctc ggccgcgagg cgccgcagga gcgggatgcc gaccatctcg ttggggaagt
      841 acggcgcctg ccgaacgggg atcgggtgga aggtcttctc gaccagcggg ccgtagcgct
      901 gctgcgcctc gtaccagtcg cggaagtagt gccagtcctg gtcgtcgggg aggacgcggt
      961 tcgcgaccac cagatcggtc gggtagttgt agaggtggaa gtaggtgaag gagcgttgcg
     1021 cctccttgat caccacctgc tccagcgtca gcacgacgcg cacgctggtc ttggtgtggt
     1081 ccgtcagcag gtcgtgcatg tgctccagcc ggccgaacag ctcctccccc gcgttgaaca
     1141 cctcgtcgcc gggcatcggc atgccgacga cgcgctggat gaggggcccg gtgagcttgg
     1201 tgatcttgcg gccgatgggc tcgatcttct ccatccacca gcgtgccgcc tccgggaggc
     1261 tcaggagccg cagcgtctcg ccggtggggg ccgcatccac gaccacgacg tcgtagttgc
     1321 ccgagtcgtg gtggtcggcg atccacagca gcgagccgac ctcgtccatg ccggggagca
     1381 ccgtcatctc ctcggccatc acctcgtcga ggccccgcca gcgcaggacc gatgcggcgt
     1441 acgcctggat cttgccccag tagcgcgcga cgttgaagaa gacgtccgat tcctgcgccc
     1501 acaggttcgg cgcgatctcc ttcggcgtcg gcccgagctc gacgttgaag gcatcgccca
     1561 gcgagtgcgc gatgtcggtc gacatcacga tcgtgcggta tccccggtcg gcgcaggcca
     1621 gcgcggtggc ggcggccacg ctcgtcttgc cgacgccgcc cttgcccgtg tagaccagaa
     1681 tgcgcgcgct caccgatgcc ccctgcgggt aggttggaga ggcggattct acgcggccgc
     1741 gcgcgccgcc gatgcgtcgc tgggcgctgc cgacgcggtg ccggctggga ccgaagccac
     1801 gcgccgtggg ggatgggcca ctcggccctc ggaagcggtg catcggcggc tgataatccg
     1861 gtcatgagaa ggttcctgct cgggttggtt gccaccctgg tcgtcgcgtt cgtcgtcatc
     1921 cagctggtgc cgtacgggcg cgcgcacgac aacccgccgg tcgcctcgga gccgaactgg
     1981 gactcgccgc ggacgcgcga gatggccgtg accgcctgct tcgactgcca cagcaacgag
     2041 acgagctggc cgtggtac
//
LOCUS       DASTYP010000057        13882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1314718, whole genome shotgun sequence.
ACCESSION   DASTYP010000057 DASTYP010000000
VERSION     DASTYP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314718"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFV59_02830"
     CDS             <1..320
                     /locus_tag="VFV59_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324796.1"
                     /translation="IGLLITASIGAFNVSWAVGLIPRAWREAPFLVVLVTRSLVWLAL
                     IAIGVSLPLLTIAGVSFADLVDQQFVISVAASFAAALLANFLGQVNGLLGRGVLLGLI
                     LGR"
     gene            339..629
                     /locus_tag="VFV59_02835"
     CDS             339..629
                     /locus_tag="VFV59_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324797.1"
                     /translation="MRVFLLIDLRESTQIAARLGNLRYHAFFSRFISDVSASVVRYRG
                     EVHRYVGDEVILTWTAKDGLRGAACVRAAFAIADSLEAARTAYEADFGVIPG"
     gene            complement(1451..1527)
                     /locus_tag="VFV59_02840"
     tRNA            complement(1451..1527)
                     /locus_tag="VFV59_02840"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1491..1493),aa:Glu,seq:ctc)
     gene            complement(1703..2569)
                     /locus_tag="VFV59_02845"
     CDS             complement(1703..2569)
                     /locus_tag="VFV59_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5324798.1"
                     /translation="MVQAIKRDEVFIEPEGIHVETHQPERRSRKPPLLLVHGVLTGSW
                     LWTEMAIYLAERGWEAHAMNLRGHFTSDVADLDETHMRDYAHDVGVAARQIGRPCVLV
                     GWGIGALAALMHAERHPVLGLILLAPSPPAGALPRRPTQHELRSVPAVYDAAWFGWAG
                     SPDELRERMPDMDDEDLEKMTELMAGARESGIARRERMQGVAVDMARLAGTPSLVIGA
                     GLDENVHPTEARRTADLLGATYEFFPSASHFGLVMGERSWPEVAQSVHGWLEERRHAM
                     AAAAGELVAGRR"
     gene            complement(2579..3931)
                     /locus_tag="VFV59_02850"
     CDS             complement(2579..3931)
                     /locus_tag="VFV59_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEU5324799.1"
                     /translation="MPRVAAFRGLHYALDRYGAPTVPPRVALPEDADAPPVRLADLTD
                     VACPPYDVIDAERQRALMAQDPHNAVRLELNASDDPHAAAAEALSRWCDEGILERREE
                     PAAYYYRHGTASAPNELTVEGVVVRVLLEPWGGGIRPHEHTMPGPKADRLALLHATRT
                     QLSPIVAVHFDRSERYHHLMSRPWSDEWRARDHDGILHQLAAIEPDERLLNHLGRQTL
                     FIADGHHRYETALAYQAEVRADPAFASAAPGELAADWIMMMLVNAELAELEIRPTHRL
                     ILDADADALRSLADGDALFEAIPLDPAELEGALTERADDETPAFGLVLPGGDGFLLVG
                     DPDGLADRLRRELMSSAVRALDLSALHVAVLEDRLGITPADVAAGRHLAYTRDEADAR
                     ERVARGEAAAAILVRPTRLDQMAAVASAGDVMPQKSTYFYPKLLTGMVFNPLSDIPED
                     "
     gene            complement(3937..5061)
                     /gene="queG"
                     /locus_tag="VFV59_02855"
     CDS             complement(3937..5061)
                     /gene="queG"
                     /locus_tag="VFV59_02855"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU5324800.1"
                     /translation="MTDAPGLDRQQLAAMAAAHGLELVGVSAADPLSDDRGRMERAVA
                     EGRMARMGWMGGTRPEVATDPRRLDPEARSVIVVAAPYVGEERGAWDPEPDAVRSALL
                     EALGSLDGPGPRGRVARYAVGTDYHHALRDRLRLLAAELRASGLPAGEVAYVDDRPLA
                     ERAFAERGGLGWIGKNTNLLTHARAGSWVFLGALLTSADLPADEPVRTSCGSCTRCLT
                     GCPTGALVAPRTMDARRCISYLTIEHPGVLDGWEASALGDWVFGCDVCQEVCPVNADA
                     ADDGPLLVPLLPLVAWLLGTGTRAFERALGHTALRRAGRHRLLRNAIAVLANGPMEPP
                     VEALLRRALDDRRPEVREQARRALLRHDRAVPEGRGSPVS"
     gene            5207..6187
                     /gene="argF"
                     /locus_tag="VFV59_02860"
     CDS             5207..6187
                     /gene="argF"
                     /locus_tag="VFV59_02860"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5324801.1"
                     /translation="MTASLTPPPARLHAPSDFLTLDAFEPDALRALLDLSEAMKTDPK
                     LHTGALAGGKVGMIFHKPSTRTRVSFEAAAWRLGMLPIALRPDELQLGRGETIGDTAR
                     TLSRYLDAITIRTFSQASVEELAAFASIPVINALTDEHHPCQVLADLLTLREEFGTLD
                     GLRVAFVGDGDNVCASLIQGAALSGFELRVATPAGYAPDPEIVERARAAAAATGGSII
                     LTGDPAAAADGADAIYADVWTSMGHEAEQEARRAAFAGFTIDDALMARARPGAIFLHC
                     LPAHRNEEVTDAVIDGPASRVWPQAENRMHTEAALLYALITGTLESSRPG"
     gene            complement(6191..8092)
                     /locus_tag="VFV59_02865"
     CDS             complement(6191..8092)
                     /locus_tag="VFV59_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5324802.1"
                     /translation="MTDPTAPSELGLHLERQMRAQERQLVWARFGTLVAAGIALLLLA
                     RDLPAVPVLLGVLAAEAVYTSLVPFLVARFPAREVGIVSTAIDMAAVTVAAYAASNAL
                     DIYLFYGLVILGTALRFGFGAAVWSAVIMSALYAVVVVVGSDPDAMVRDLLPTRVAFL
                     LGFGLVAGLFSRILIGRATENARLQQRLAEEERDRERRAEAELLARIGRDAAASLDRG
                     ATHRALVEGAAPVLGDATLLLAVDDATRRLTVEAADGPDPDLVAAWRQGLEVRRPRVG
                     EGIAGAVAATAASREGAVEESTGDPDALRAAGAAWMYATPILAGGRLLGVLATARRAA
                     PEDGRITRLADMVAERAGPAVQNAELWADLQRRMAAEQAAQRVKDDFLSIVSHELRTP
                     LTSIQGYSQLLEGRLSGERDASAKELAHLRVIRSQVSRMRRLVDDLLDVSRIDRRGGV
                     SIETADFDLAEEVRDAVARARREHPDQSIDVTAPDVLPVHADRDRIDQVLGNLIENAV
                     KYSPDGGPIRVECERRGGEVEVRVTDEGIGIPPEHRDHVFERFYQADGGAGGRRFGGL
                     GLGLYITRAIVDAHGGRIWAAANPDGPGSVFGFRMPRIAMEPVTPGIAPTGEPPAFVL
                     RHRDGDGGA"
     gene            8231..10672
                     /locus_tag="VFV59_02870"
     CDS             8231..10672
                     /locus_tag="VFV59_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324803.1"
                     /translation="MSDLPTVRSDRRRPRASVAATVLTVLTLLLAAFPMPVLAASSVS
                     IEARPLLGGRYEVGGWAAVAVSLVNEGEPTEGYLEADGEDSVVRRFVEMPAGARKEVT
                     LYVAPEAFQRRITVRYEEPNGTVEATVEVRVFEQTGDQVAIVGEGAGTLRPQLPTATG
                     PGSPEPISIGVADLPDRLEPLRGLSGIVWAGDSSALTEDQRRSLASWVAEGGQLVVVG
                     GPDWQARTDAFADLLPLESLTAVDGVALASLADWAGGSEPPVATATVSSGTLRPDARQ
                     LVLDGQGNTLVSMRDVGAGRVILLGPDLASEPFRGWTDGPALWGRLIPSNALWEQYFG
                     SGMPPREEMANSMSQALGNLPSLEVPPVELLLLVVVAYILLIGPVNYLVLRRLDRREL
                     AWVTAPVLVVLFSATSYGIGSAMKGSSVIVNQVAVIRASTAGTAANVETYAGVFSPDR
                     ATYDVRVDADALLGLLNTSNFQPTPQNRADVVVEQGDPARMRELAVGVFGFESVRADA
                     VTEHAAALAIEWERDGEDLLGVVTNVGDKPLEDVAYISRSGGEMVGDLAPGEAGRFEV
                     TGSNFNASSASDQVYGFGGFDPANPDQREIAARRSIIDALVGYGSFMPPGTDLPSTGR
                     GPYVIGWSASPGPLPVTVEGVEAQQYAQSVEVITVRPTLGTGELTIGPGMMTVSLVET
                     EGDASVPSPGSIMIGQGSAVVDVSLPLEASGMAVEQVEVIAAPDPGMMVQDGGGFGGF
                     WPAGYTFEVLDPSTGTWTLLGNLDQQSRYTIDDPATVITDAGRIRLRITGTADQAFGQ
                     NSFFVSAEVSGVLDQ"
     gene            10669..11643
                     /locus_tag="VFV59_02875"
     CDS             10669..11643
                     /locus_tag="VFV59_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324804.1"
                     /translation="MTPVVETRGLVKRYGDQLALAGVDLVVGPGEIYGLVGPNGAGKT
                     TTMKILATLLAPTAGEAYVTGIPVDADPLEVRRRIGYMPDFYGVYDDLRVWEYLDFFA
                     RCYGVPANRRVTMIGELLEIVGLTDKRDAYVESLSRGMRQRLCLAHTLVHDPALLILD
                     EPASGLDPRARVEMREILRELRAMGKTILVSSHILPELGEMCTGVAIIDHGRLLRSGR
                     IDEIERSLRATALLRIDLLADESGVTAAREWLGTDPRVGDVLHVESEAGGPVRLEVAF
                     DGGPEAQGDLLGAMVAAGHRVIAFAPATSDLEEIFLKVTGQDEPEVAA"
     gene            11640..12770
                     /locus_tag="VFV59_02880"
     CDS             11640..12770
                     /locus_tag="VFV59_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5324805.1"
                     /translation="MTATAVPARPSRRSALREFGGSVATIAVKELRSRMRGRRAFIVL
                     TIYLGLLALLTYGIYVIVAPNARSMGVFGGSTPNASALIGQSIFTFMSIFQLILVAFI
                     APAFTAGQISLEREKQTLDLLVSTPMRPAAIVLGKLAAALAFVVLMIVAAIPITAIVL
                     MYGGASIDDIVRQQLVLLASATALGSVGLFFSALLKRTQAATVLTYITMLFLTLGTAL
                     LFAFWTIVLIQSNAGNPLAGSQRAPEQVLWVNPGVAMLDVVANTEPGGGAGVASFLNQ
                     LRGESPSFTGGRVDCIGPDCVAVDDFGNPVGQPFDIASTGYWWPRIAITMLVLSAILT
                     FAATRLVVPAGMRWAFRRRAPRRAAAPVGTQATTDEIEEAHP"
     gene            12767..>13882
                     /locus_tag="VFV59_02885"
     CDS             12767..>13882
                     /locus_tag="VFV59_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324806.1"
                     /translation="MTDRLIAQAVTERLGPESLARLRQLRRRLWWRRSARIGLVIAAG
                     TVLGVALVQLASRAFPFEWAPLVQLAIGLVGLLTWVAASIGRRPTLVQTAWLADEELG
                     LRQRLGTALELAAHEDQDVLQERQLADARARLAAVEVRRAFRPRVARRPAVVAGVGLA
                     MTLLLVAWPNPQDAVIEQRRASREAAEQVAERVEEVADEVGEENVENPDPRREELERQ
                     LRELARQLREGGDDREETLARIGSVQEELSRQTDPRAAERDAGLSQLARAASRAATGD
                     EEANPEGDPEQAATDLEELADRAADLPENEMGQRADALRQAAQSGAASQPAAAAALTE
                     AADALDEAARTGNEQDRQAAEEALRRAADALRRAESDR"
BASE COUNT         1931 a         5076 c         4915 g         1960 t
ORIGIN      
        1 tgatcggcct gctgatcacg gcgagcatcg gcgccttcaa cgtgagctgg gcggtcgggc
       61 tcattccccg tgcgtggcgc gaggccccgt tcctcgtggt gctcgtcacc cgcagcctcg
      121 tgtggcttgc gctcatcgcg atcggggtca gcctgccgct cctcaccatc gcgggggtct
      181 cgttcgcgga cctcgtggac cagcagttcg tgatctccgt tgccgcgagc ttcgccgccg
      241 ccttgctggc caacttcctg ggccaggtga acggcctcct cggccgcggc gtgctgctgg
      301 gcctcatcct cggccgctag caccggcctc gcgaggaagt gcgcgtcttc ctgctcatcg
      361 acctgcgcga atccacgcag atcgccgcgc gactcggcaa cctgcgctac cacgccttct
      421 tcagccgatt catcagcgac gtttccgcca gcgttgtgcg ctaccgcgga gaggtgcacc
      481 gctacgtggg tgacgaggtc atcctgacct ggaccgcgaa ggacggtctg cggggcgccg
      541 cctgcgtccg cgcggccttc gccatcgccg actccctcga ggcggcccgc acggcgtacg
      601 aagccgactt cggcgtgatc ccgggttgac gcaacggagg agcggccgga ggcttggctt
      661 catgactgcg agacatctgt gggccgcttg gcgtccaaat accatgctca ggcgagcttc
      721 ggccgcggtc cttgtggcgg ctgcactggt gttgctcgtc cctgcggtca tcctgccagt
      781 tgtcgctccg gacgcagagg cggtccctgc cgtcgaacgt ctgcttcccg atgccaagcc
      841 gtacttagcg cgacaactgg acctcggcct ggcacatgtg cgctattcgg gcgccgaact
      901 gcgagcccgt gatcatctag tgatccttga attcgaaatc cggccgttcc catacgtcgc
      961 ctctgagggc gcgtatctcg tttctcgatg cactccactg gagcagcttg acccgaacac
     1021 gatgggtggc gggagaggcg tcaccgactt cgctacagat gccgaactcg cgtacgtccg
     1081 atctgacgcc caacctccgt gcgagtaagc gtcgctcgcg aggcggcact cggcaagagg
     1141 tcgatctgca gaggttgtga tctggcgtgc acgccgcggg cgcgatccga gtcgtgcgag
     1201 tggcgcatgc tcgcttcgct gggctgaaga cgacgcgctc gcggctcaga ctccctttgc
     1261 ggcaagattc gaatcagccg gttcgaaggt gcagctcggc ctcagactca cgcgctacag
     1321 gaggacacca ggcgagccga gcctagtagt tcgagcccgc tcgcgcggca ccgaaagggg
     1381 tacgaatgag ggtacggccg caacgaggga gccccgatcc gtgctcagaa tcggggctgg
     1441 acgaaggaag tggtaccgca taccggattc gaaccggtga tctccgcctt gagagggcgg
     1501 tgtcctaggc ctctagacga atgcggcacg tccggatgac gggccagagt ctaccaaggg
     1561 ctcagagtcc gagcaaacca ctgaccggtc agacgacccc cgttcggcta cccattccgt
     1621 gccctcaccg gacaggtcac tgatgatatg accgctgata gccgaggacg ggcggctcga
     1681 tcgggcgcgc cctgccccgg ggctaccggc ggccggcgac cagctccccc gcggccgccg
     1741 ccatcgcgtg acgccgctcc tcgagccagc catggaccga ctgcgccacc tccggccagg
     1801 accgctcccc catcaccagg ccgaagtggc tggccgaggg gaagaactcg taggtggcac
     1861 ccagcaggtc ggcggtgcgc cgcgcctcgg tggggtggac gttctcgtcc aggcccgccc
     1921 cgatcaccag gctcggcgtc ccggccaggc gcgccatgtc gaccgccacg ccctgcatcc
     1981 gctcgcggcg cgcgatcccc gactcacggg caccggccat cagctcggtc atcttctcga
     2041 gatcctcgtc gtccatgtcc ggcatccgct cgcgcagctc gtccgggctg cccgcccaac
     2101 cgaaccaggc ggcgtcgtag acggcgggca cgctgcgcag ctcgtgctgg gtcgggcggc
     2161 gcgggagggc gccggcgggc ggcgacggtg ccagcaggat cagccccagg acggggtgcc
     2221 gctcggcgtg catgagggcc gccagcgccc cgatccccca gccgaccagc acgcagggtc
     2281 gcccgatctg ccgcgccgcc acgccaacgt cgtgcgcgta gtcgcgcatg tgcgtctcgt
     2341 cgaggtcggc cacgtcgctg gtgaagtggc cgcgcaggtt catggcgtga gcctcccagc
     2401 cgcgctcggc caggtagatc gccatctcgg tccacagcca cgagccggtg aggacgccgt
     2461 gcaccaggag cagcggcggc ttgcgcgatc ggcgctcggg ttggtgggtc tccacgtgga
     2521 tgccctcggg ctcgatgaag acctcgtcgc gcttgatcgc ctgcaccatc ggggctcgtc
     2581 agtcctccgg gatgtcggat agggggttga agaccatgcc ggtcagcagc ttggggtaga
     2641 agtaggtcga cttctgcggc atcacgtcgc cggcgctcgc caccgcggcc atctggtcga
     2701 gtcgggtggg ccggacgaga atggcggccg ccgcctcgcc gcgcgcgacg cgctcgcgcg
     2761 catcggcctc gtccctagtg taggcgaggt gccgcccggc ggcgacgtcg gccggggtga
     2821 tgcccagccg atcctccagc acggccacgt gcagcgcgct gaggtccagg gcacgcaccg
     2881 cggagctcat gagctcccgt cgcagccgat ccgccaggcc atcgggatcc ccgaccagca
     2941 ggaagccgtc gccccccggg agcaccaggc cgaaggcggg cgtctcgtca tccgcgcgct
     3001 cggtcaacgc cccctccagc tccgcggggt cgagcgggat cgcctcgaac agggcgtcgc
     3061 catcggccag ggagcggagg gcatcggcgt ccgcgtccag gatgagccga tgggtgggcc
     3121 ggatctcgag ctcggcgagc tcggcattca cgagcatcat catgatccag tcggccgcca
     3181 gctcccccgg cgcagccgat gcgaaggcgg gatcggcacg aacctccgcc tggtacgcga
     3241 gggcggtctc gtaccgatgg tgtccatcgg cgatgaacag ggtctggcga cccaggtgat
     3301 tgagcagccg ctcgtccggc tcgatggccg ccagctggtg gaggatcccg tcgtggtcgc
     3361 gcgcgcgcca ctcgtccgac caggggcgac tcatcaggtg gtgatagcgc tcgctgcggt
     3421 cgaaatggac cgccacgatc gggctgagct gggtcctggt ggcgtgcagc agcgccagcc
     3481 gatccgcctt ggggccgggc atggtgtgct cgtgcggtcg gatgccgccg ccccacggct
     3541 cgaggagcac ccgcaccacc accccctcga cggtgagctc gttcggcgcc gatgcggtcc
     3601 cgtgccggta gtagtaggcg gccggctctt cgcgtcgttc gaggatgccc tcgtcgcacc
     3661 agcggctgag cgcctcggcc gccgcggcgt gcggatcgtc cgacgcgttg agctccagcc
     3721 gaaccgcgtt gtgcgggtcc tgcgccatga gcgcccgctg ccgctcggcg tcgatcacgt
     3781 cgtacggcgg gcaggcgacg tccgtcaggt cggccaggcg gacgggcggc gcatcggcat
     3841 cctcgggcag cgcgacacgc ggcggcacgg tcggggcgcc gtagcggtcg agcgcgtagt
     3901 gcaggccgcg gaaggcggcg acccggggca tgcgggctag gataccggcg atccccgacc
     3961 ctcgggcacg gcccgatcgt gccgcagcag cgcccggcgt gcctgctccc gcacctcggg
     4021 gcgacggtcg tccagcgctc gacggagcaa cgcctccacc ggcggctcca tcggtccatt
     4081 cgccagcacc gcgatcgcgt tccggagcag ccgatgccgt ccagcgcggc gcagggccgt
     4141 gtgacccagc gcccgctcga aggcgcgcgt gccggtgccc agcagccacg cgaccagggg
     4201 tagcagcgga accaggagtg gaccatcgtc ggccgcgtcg gcgttgaccg ggcagacctc
     4261 ctggcacacg tcgcagccga agacccagtc gccgagggcc gaggcctccc agccgtccag
     4321 cacgccgggg tgctcgatcg tcaggtagct gatgcaccgt cgcgcatcca tggtgcgggg
     4381 cgccaccagc gcgcccgtgg gacagcccgt gagacaccgg gtgcaggagc cgcagctcgt
     4441 ccgcaccggt tcgtcggccg ggaggtcggc gctggtcagg agcgcgccaa ggaacaccca
     4501 cgacccggcc cgagcgtgcg tcagcaggtt ggtgttcttg ccgatccacc cgagcccgcc
     4561 tcgctccgcg aaggcgcgct ccgccagcgg ccggtcgtcg acgtaggcga cctcaccggc
     4621 cggcagcccg gacgcccgca gctcggccgc cagcaaccgc agccgatccc gcagtgcgtg
     4681 gtggtagtca gtgcccaccg cgtaccgcgc cacgcgccca cgcggccccg gcccgtccag
     4741 cgacccgagc gcctccagca gggccgaccg cacggcgtcc ggttccggat cccacgctcc
     4801 ccgctcctcg ccgacgtacg gcgccgccac cacgatcacc gaccgcgcct cagggtccag
     4861 ccggcgcgga tcggtggcca cctccggccg ggtgccgccc atccagccca tgcgcgccat
     4921 ccggccctcg gccaccgcgc gctccatgcg accgcggtcg tccgacagcg gatcggcggc
     4981 gctcacgccc accagctcca acccgtgggc ggcggccatc gcggcaagct gctggcgatc
     5041 caggcctggc gcatcggtca tgtcgtccag cctagccatg ccggctgacc ggaatggacc
     5101 gatctcgccg ggccatccgg cactggaccg ccagcggtcg ctcccgatcc tcggagggag
     5161 gccgcgcacg caagcgtcgg ccaccatcgg cgaacaggag acgcgcgtga ccgcttcgct
     5221 gactcctccg ccggcgcgcc tccatgcgcc gagcgacttc ctgaccctgg acgcgttcga
     5281 gcccgatgcg ctccgcgccc tgctggacct ctcggaggcc atgaagaccg atccgaagct
     5341 gcacaccggt gcgctggccg gcggcaaggt cggcatgatc ttccacaagc cgtcgacgcg
     5401 gacgcgggtc agcttcgagg ccgcagcgtg gcggcttggg atgctgccga tcgcgctccg
     5461 gccggacgag ctgcagctgg gccgcggcga gacgatcggc gacaccgccc ggacgctgtc
     5521 gcgctacctg gacgccatca ccatccgcac cttcagccag gcatcggtcg aggagctggc
     5581 cgcgttcgcc agcatcccgg tcatcaacgc gctcaccgat gagcaccacc cctgccaggt
     5641 cctggccgac ctgctgacgc tgcgcgagga gttcggcacg ctcgacggcc tgcgcgtcgc
     5701 gttcgtcggc gacggcgaca acgtgtgcgc ctcgctgatc cagggcgcgg ccctgtccgg
     5761 cttcgagctg cgcgtcgcga cgcccgccgg ctacgcaccc gacccggaga tcgtcgagcg
     5821 ggcgcgcgcg gccgccgcgg cgaccggcgg ctcgatcatc ctgaccggcg atcccgccgc
     5881 ggccgccgat ggcgccgatg cgatctacgc cgacgtctgg acctcgatgg gccacgaagc
     5941 cgaacaggag gcccgacgcg ccgccttcgc cggcttcacc atcgacgacg cgctcatggc
     6001 gcgggcccgc cctggggcga tcttcctgca ctgcctgccc gcccaccgca acgaggaggt
     6061 caccgatgcg gtgatcgacg gccccgcgtc ccgcgtctgg ccgcaggccg agaaccgcat
     6121 gcacaccgag gcggcgctgc tctacgccct gatcaccggg acgctggagt cgtctcgccc
     6181 gggctgagcc tcaggcgccg ccatcaccgt cgcggtgacg caggacgaac gccggcggct
     6241 ccccggtcgg cgcgatgccc ggggtgaccg gctccatcgc gatccgcggc atgcggaatc
     6301 cgaacacgga gccgggtccg tcggggttgg ccgccgccca gatgcggccg ccgtgcgcgt
     6361 cgacgatggc ccgcgtgatg tacagcccga ggcccaggcc gccgaaccgg cgaccgcccg
     6421 cgccgccatc ggcctggtag aagcgctcga agacgtggtc gcggtgctcg ggcgggatgc
     6481 cgatcccctc gtccgtcacg cgaacctcga cttcgccgcc gcgccgctca cactcgacgc
     6541 ggatcggtcc gccgtccggc gagtacttca ccgcgttctc gatcaggttt cccagcacct
     6601 ggtcgatccg atcgcgatcc gcgtgaaccg gcaggacatc cggggccgtg acgtcgatcg
     6661 actggtcggg gtgctcgcgg cgtgcccgcg cgaccgcgtc ccggacctcc tcagccaggt
     6721 cgaagtcggc cgtctcgatg ctcacgccac cgcgccgatc gatccggctg acgtccagga
     6781 ggtcgtccac caggcggcgc atgcggctga cctgggatcg gatgacgcgg aggtgcgcga
     6841 gctccttggc gctcgcgtcg cgctcgccgc tcaggcggcc ctccagcagc tgcgagtagc
     6901 cctggatgct cgtgagcggc gtccgcagct cgtggctgac gatcgagagg aagtcgtcct
     6961 tcacccgctg ggcggcctgc tccgccgcca tgcgacgctg gagatccgcc cacagctcgg
     7021 cgttctgcac cgccggtccg gcacgctcgg ccaccatgtc ggcgagccgc gtgatacggc
     7081 cgtcctcggg cgccgctcgc cgggccgtgg ccagcacgcc cagcagccgt ccgcccgcca
     7141 ggatgggcgt ggcgtacatc caggccgcac cggcggcccg cagcgcgtcc ggatcgccgg
     7201 tactctcctc cacggcgccc tcccggctcg cggccgtcgc ggccaccgcc ccggcgatgc
     7261 cctccccgac gcggggacgc cggacctcga gcccctgccg ccaggcggcc accaggtccg
     7321 gatccggccc gtcggccgcc tcgacggtca gccgacgcgt cgcgtcgtca acggccagca
     7381 gcagcgtggc gtcgcccaga accggggcgg cgccctccac cagcgcccgg tgcgtcgccc
     7441 cgcggtcgag ggatgcggcg gcgtcacgcc cgatccgcgc cagcagctcc gcctcggccc
     7501 gacgctcgcg gtcccgctcc tcctccgcca ggcgctgctg caggcgcgcg ttctcggtcg
     7561 cacggccgat gaggatgcgg ctgaagaggc cggccaccag gccgaagccc agcagaaatg
     7621 cgacgcgcgt cggcaagagg tcgcggacca tcgcgtccgg gtcgctgccg accacgacga
     7681 ccaccgcgta cagcgcgctc atgatgacgg ccgaccagac ggcggcaccg aatccgaagc
     7741 gcagggccgt gcccaggatg acgaggccgt agaagaggta gatgtcgagc gcgttcgacg
     7801 cggcgtacgc ggccacggtg accgccgcca tgtcgatcgc cgtgctgacg atccccacct
     7861 ctcgcgccgg gaagcgcgcc accaggaacg ggaccagcga ggtgtagacc gcttccgcgg
     7921 cgagcacccc gagcagcacc gggacggcgg ggaggtcgcg cgccagcagg agcagcgcga
     7981 tcccggcggc cacgagggtc ccgaacctgg cccagaccag ctggcgctcc tgggcgcgca
     8041 tctggcgctc caggtggagg ccgagctcgg acggggcggt gggatcggtc atgcgcagcg
     8101 cgtgcactct agcacccggc cgatcggctc ccgtacactc gagcgaccga gcaagccgtg
     8161 caactccggc acggcccggc gcgtctaccg gggaacccac ctcgaccagg ggagaccgca
     8221 accagggccg atgtctgacc tgcccaccgt ccgttccgat cggcgccgtc cgcgcgccag
     8281 cgtggccgcc accgtgctca ccgtgctgac gctgctgctc gctgccttcc cgatgccggt
     8341 cctcgcggcc agctcggtgt ccatcgaggc gcgcccgctg ctgggtggac gctacgaggt
     8401 cggcgggtgg gccgccgtgg ccgtgtccct ggtcaacgag ggcgagccga cggagggata
     8461 cctggaggcg gacggcgagg actccgtggt gcgccgcttc gtcgagatgc cggccggcgc
     8521 gcgcaaggag gtcacgctgt acgtcgcccc cgaggccttc cagcggcgga tcacggtccg
     8581 ctacgaggag ccgaacggta cggtggaggc caccgtcgag gtgcgcgtct tcgagcagac
     8641 cggcgaccag gtcgcgatcg tgggcgaggg cgccggcacg ctgcggcccc agctgcccac
     8701 ggcgacgggg ccaggttcac cggagcccat cagcatcggg gtcgccgacc tgcccgaccg
     8761 cctggagccg ctgcgcggcc tgtcgggaat cgtctgggcc ggcgacagca gcgcgctgac
     8821 ggaggaccag cgccgcagcc tggcaagctg ggtcgccgag ggtggccagc tcgtggtcgt
     8881 cggggggccg gactggcagg cacggaccga tgcgttcgcg gacctgctcc ccctcgagtc
     8941 cctcaccgcc gtggatggcg tcgccctcgc cagcctggcg gactgggccg gcggcagcga
     9001 gccaccggtc gcgaccgcca ccgtctcgtc cggcacattg cggcccgacg ccaggcagct
     9061 cgtcctggac ggccagggca acacgctggt ctccatgcgg gacgtcggcg ccggacgcgt
     9121 gatcctgctg ggccccgacc tggccagcga gccattccgc ggctggaccg acgggccggc
     9181 gctgtggggc cggctgatcc cctccaacgc gctctgggag cagtacttcg gcagtggcat
     9241 gccgccgcgc gaggagatgg ccaactcgat gagccaggcc ctgggcaacc tgccctcgct
     9301 cgaggtgccc ccggtcgagc tgctgctcct cgtcgtggtc gcctacatcc tgctcatcgg
     9361 gcccgtgaac tacctcgtcc tgcgacggct cgaccgccgc gagctggcgt gggtgacggc
     9421 gccggtgctg gtcgtcctgt tcagtgcgac ctcgtacggc atcggctcgg ccatgaaggg
     9481 cagctcggtc atcgtcaacc aggttgcggt catccgcgcc tcgaccgccg gaaccgcggc
     9541 gaacgtggag acgtacgccg gcgtcttctc gcccgaccgc gccacctatg acgtgcgcgt
     9601 cgacgccgat gccctgctgg gcctgctcaa cacctcgaac ttccagccga cgccgcagaa
     9661 ccgggccgac gtcgtggtcg agcagggcga cccggcgcgc atgcgcgagc tggcggtcgg
     9721 cgtcttcggc ttcgagtccg tccgcgccga cgcggtcacc gagcacgcgg cggccctggc
     9781 catcgagtgg gagcgcgacg gcgaggacct gctcggcgtc gtcaccaatg tcggcgacaa
     9841 gccgctggag gacgtcgcct acatctcgcg cagcggcggc gagatggtcg gcgacctcgc
     9901 ccccggcgag gccggacgct tcgaggtgac cggatcgaac ttcaacgcgt cgtcggcatc
     9961 cgaccaggtg tacggcttcg gcggcttcga cccggcgaac cccgatcagc gggagatcgc
    10021 cgcgcggcgc agcatcatcg acgcgctggt cggctacggc tcgttcatgc cgcccggcac
    10081 ggacctgccg agcaccggcc gaggaccgta cgtgatcggc tggagcgcgt cgccggggcc
    10141 gctgccggtc acggtcgagg gcgtcgaggc ccagcagtac gcgcagtcgg tcgaggtgat
    10201 cactgttcgc ccgacgctcg gtaccggtga gctgaccatc ggtcccggaa tgatgacggt
    10261 ctcgctggtg gagaccgagg gcgacgcgtc ggtgccgagc cccgggagca tcatgatcgg
    10321 gcagggatcg gcggtcgtcg acgtcagcct gccgctcgag gcgtccggca tggccgtcga
    10381 gcaggtggag gtcatcgcgg cgcccgaccc cggcatgatg gtccaggacg gcggtggctt
    10441 cggcggcttc tggcccgccg gctacacgtt cgaggtgctc gacccatcga ccggcacgtg
    10501 gacgctgctc ggcaacctcg accagcagag ccggtacacc atcgacgatc cggcgacggt
    10561 catcaccgat gcgggtcgca tccgcctgcg catcaccggc accgccgatc aggccttcgg
    10621 ccagaacagc ttcttcgtct cggccgaggt atccggggtg ctggaccagt gacccccgtc
    10681 gtcgagacgc gcggcctcgt gaagcgctac ggcgaccagc tcgccctggc cggcgtcgac
    10741 ctggtggtcg gcccgggcga gatctacggc ctggtcggcc cgaacggcgc cggcaagacg
    10801 accaccatga agatcctcgc cacgctgctg gcgccgacgg ccggcgaggc ctacgtgacc
    10861 ggcatcccgg tcgacgcgga tccgcttgag gtccggcggc gcatcggcta catgcccgac
    10921 ttctacggcg tctacgacga cctgcgcgtc tgggagtacc tggacttctt cgcacgctgc
    10981 tacggcgtgc cggccaaccg tcgcgtgacg atgatcggcg aactgctcga gatcgtcggc
    11041 ctcaccgaca agcgcgatgc gtacgtcgag agcctgtcgc gcggcatgcg ccagcgcctg
    11101 tgcctggccc acacgctggt ccacgacccg gcgctgctca tcctggacga gcccgcgtcg
    11161 ggcctcgacc cgcgtgcccg ggtcgagatg cgcgagatcc tccgtgagct gcgggccatg
    11221 ggcaagacga tcctggtcag cagccacatc ctccccgagc tcggtgagat gtgcaccggc
    11281 gtcgcgatca tcgatcacgg ccggctgctg cggtccgggc gcatcgacga gatcgagcgg
    11341 tcgctgcgcg cgacggcgct gctgcgcatc gacctcctgg ccgacgaatc cggcgtgacg
    11401 gccgcgcgcg agtggctggg aaccgatccg cgcgtcggcg acgtcctgca cgtggagagc
    11461 gaggcgggcg gccccgtacg gctggaggtc gccttcgatg gcggccccga ggcgcagggt
    11521 gacctgctcg gggcgatggt ggcggcgggg caccgtgtga tcgccttcgc accggcgacg
    11581 agcgacctgg aggagatctt cctgaaagtg accggacagg acgaaccgga ggtggcagca
    11641 tgacggcgac ggcggttccg gctcgaccgt cgcggcgctc ggcgctgcgc gagttcggag
    11701 gctccgtggc gaccatcgcg gtcaaggagc tgcggtcgcg catgcgcggc cggcgcgcct
    11761 tcatcgtcct gaccatctac ctgggcctgc tcgcgctgct gacctacggc atctacgtca
    11821 tcgtcgcgcc caacgcgcgg tcgatgggcg tgttcggcgg ctcgacgccg aacgcatcgg
    11881 ccctcatcgg ccagtcgatc ttcaccttca tgtcgatctt ccagctgatc ctggtcgcct
    11941 tcatcgcgcc ggcgttcacg gccgggcaga tcagcctgga gcgcgagaag cagacgctgg
    12001 acctgctggt cagcacgccg atgcgtccgg cggcgatcgt gctcggcaag ctcgcggccg
    12061 ccctcgcctt cgtggtgctg atgatcgtgg ccgccatccc gatcacggcg atcgtgctga
    12121 tgtacggcgg cgcgtcgatc gacgacatcg tgcggcagca gctcgtgctg ctggccagcg
    12181 ccaccgccct ggggtccgtc gggctcttct tctcggcgct cctcaagcgg acgcaggcgg
    12241 cgacggtgct gacctacatc accatgctct tcctgaccct cggcacggcg ctgctgttcg
    12301 ccttctggac gatcgtcctg atccagtcga acgccggaaa cccgctggcc ggatcccagc
    12361 gcgcgccgga gcaggtgctc tgggtcaacc ccggggtggc catgctcgac gtcgtggcca
    12421 acacggagcc cggcggcggc gccggcgtcg cctcgttcct gaaccagctg cgtggcgagt
    12481 cgccatcgtt cacgggtggc cgggtcgact gcatcggccc cgactgcgtc gcggtcgacg
    12541 acttcgggaa tccggtcggg cagcccttcg acatcgcctc gaccgggtac tggtggccgc
    12601 gcatcgcgat caccatgctg gtcctctccg ccatcctgac cttcgcggcg acgcgcctgg
    12661 tggtgccggc gggcatgcgc tgggcgttcc gccggcgcgc gccccggcgg gccgcggcgc
    12721 cggtcggcac ccaggccacc accgacgaga tcgaggaggc ccacccgtga ccgaccgcct
    12781 catcgcgcag gccgtcacgg agcgcctcgg ccccgagagc ctggcccggc tgcgacagct
    12841 ccgacgccgg ctgtggtggc gacggtcggc ccggatcggg ctggtgatcg cggccggcac
    12901 ggtactcggt gtcgcgctcg tccagctcgc gtcgcgggca ttccccttcg agtgggcacc
    12961 gctggtccag ctggcgatcg gccttgtggg gttgctgacg tgggtcgccg cgtccatcgg
    13021 tcgccgcccg acgctcgtcc agaccgcgtg gctggccgac gaggagctgg gtctgcggca
    13081 gcgcctgggg acggcgctcg agctggcggc gcacgaggac caggacgtgc tccaggagcg
    13141 gcagctggcc gacgcccgag cccggctggc ggccgtcgag gtgcggcgcg ccttccgacc
    13201 acgtgttgct cgacgaccgg ccgtggtggc cggggtgggg ctggccatga cgctgctgct
    13261 ggtcgcatgg ccgaacccgc aggacgcggt gatcgagcag cgacgggcgt cacgcgaggc
    13321 cgcggagcag gtggcggaac gggtcgagga ggtcgccgac gaggtcggcg aggagaacgt
    13381 cgagaaccct gacccacgcc gcgaggagct cgagcgccag ctgcgcgagc tcgcgcgaca
    13441 gctgcgcgag ggcggtgacg atcgcgagga gaccctggcg cgcatcggtt cggtgcagga
    13501 ggagctgtcg aggcagaccg atccgcgtgc cgcggagcgg gacgccggct tgagccagct
    13561 cgctcgggcg gcttcacggg cggccacggg tgacgaggag gcgaacccgg agggtgaccc
    13621 cgagcaggcg gccaccgacc tcgaggagct cgccgaccgc gccgccgacc ttcccgagaa
    13681 cgagatgggg caacgggccg atgcgctgcg ccaggcggcc cagagcggcg ccgcctctca
    13741 gcccgctgcg gcggccgccc tgaccgaggc cgcggacgcg ctcgacgagg cggcgcggac
    13801 cggcaacgag caggaccgcc aggcggccga ggaggcgctc cggcgcgcgg ccgatgcgct
    13861 gcgacgtgcc gaatccgacc gt
//
LOCUS       DASTYP010000058         6430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_732788, whole genome shotgun sequence.
ACCESSION   DASTYP010000058 DASTYP010000000
VERSION     DASTYP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732788"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1989)
                     /gene="ftsH"
                     /locus_tag="VFV59_02890"
     CDS             complement(34..1989)
                     /gene="ftsH"
                     /locus_tag="VFV59_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5324807.1"
                     /translation="MTSRIVRNSVVLVVLAVFGLAVLWTFMGDGSQSADYGYGEMLAD
                     ARDGNIKSITQQGQRLNAVLNSGQERTAVAPSELTNVYADLGCADAASGDFNCETFTV
                     QEESAAGGILTLLITALLPVLLIGGFIFFMMRQAQGTNNQAMSFGKSRARMFLGNKTV
                     VTFNDVAGVDEAKQELTEVVEFLKYPEKFNSLGARIPRGVLLVGPPGTGKTLLARAVA
                     GEAGVPFFSISGSEFVEMFVGVGASRVRDLFEQAKRNSPCIVFVDEIDAVGRQRGAGL
                     GGSHDEREQTLNQILVEMDGFDTNTNVIVVAATNRPDVLDPALLRPGRFDRQVVLDRP
                     DIKGRKEILNVHIKGKPLDKTVDIEALARRSPGFSGADLANLVNEAAILAARRNKKSV
                     GMAEFNEAIDRVVAGPERRSRIITEEEKEIIAYHEGGHAVVQRVLPKCDPVTKVTIVS
                     RGMALGYTMSLPSEDRYLHSKSEFEDKIAGMLGGNVAETIVFGDTTTGSSNDIEKATG
                     LARAMVTQYGMSEKLGPLAFGKKDEMVFLGREISEQRNYSDEVAAKIDAEVRSIIDNA
                     FARAKEALTSHREVLDRLASLLVEKETIEHEEFEALFDGVLPPRTGTPTPRKIGVEAT
                     GGEGVTEDVEPEVEGKRPRPGTAPSPA"
     gene            complement(2153..3079)
                     /gene="tilS"
                     /locus_tag="VFV59_02895"
     CDS             complement(2153..3079)
                     /gene="tilS"
                     /locus_tag="VFV59_02895"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5324808.1"
                     /translation="MRGARALGVPADAHLILAVSGGSDSMALLHGATHACRTEAVAWR
                     LTVAHLDHALRPASADDATFVNDAAADLGLRSEARRVDVARLAAAEGRSVEDAGREAR
                     YRFLARLAGDYAWIATAHTADDATETVLLNLLRGSGLAGARGMPARRGRIVRPLLDQR
                     RATLRQLLDGAGITYRVDPSNDEARYLRNRVRAELLPLLEELRPGAVDRIGQFARLAA
                     DDDALLDEVAAAELDRRRAADGSIDWRDPPPAALGRRVLRLAIGDPAASAERVEALLE
                     AATGPRGGLTIELGGGRRASVRQRRITVLDDR"
     gene            complement(3115..3189)
                     /locus_tag="VFV59_02900"
     tRNA            complement(3115..3189)
                     /locus_tag="VFV59_02900"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3155..3157),aa:Met,seq:cat)
     gene            complement(3283..3936)
                     /locus_tag="VFV59_02905"
     CDS             complement(3283..3936)
                     /locus_tag="VFV59_02905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5324809.1"
                     /translation="MEPMHARHPLRDLLERAARGHPPPADGSVAVLPSPPGHADAVVA
                     FTAHHVVAAAVDPEAVRQRLGGADLGAPMGASFLAWLGEQLRAEPGSLDVVLVAAGRG
                     SPSVELRSVSAADHARVARAERYRSEVRAWTDAEERGVVTLGRGLAGRLEVSVELDPS
                     ARGRGIGRRMIAAALALVPSGERVFAQAAPGNAASLRAFLAAGFVPIGAEVLFLRGR"
     gene            complement(3948..4964)
                     /locus_tag="VFV59_02910"
     CDS             complement(3948..4964)
                     /locus_tag="VFV59_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324810.1"
                     /translation="MAAQPDAVRVAAAADTEWLRRRGPGWLVVAGKELAETLTSLRFA
                     VLLVILGLAALAPVYATSGAIRDAAEQASGAAALFLALFTLGADPVPSFVGLVGFVAP
                     LLGIAFGFDAINGERAQGTLARLLSHPIHRDDLINGKFAAGLAAIAFAVVSMALLVAG
                     LGIVRLGIVPSVGEVARMLAWVVVTILYIGLWLAIAMLCSVLSRRAATSALIAIGLWL
                     ALSLFGVLLAQLIAGFLDPISGSVGTEAALRHAQLQQQLSIVNPGTVYAQATLALLNP
                     GATAVVVPGLAEAIQLSQQIPTQLSLQQSLLIVWPQVVGLLAAMVLCFVASYIVFLRQ
                     EVRA"
     gene            complement(4948..5748)
                     /locus_tag="VFV59_02915"
     CDS             complement(4948..5748)
                     /locus_tag="VFV59_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324811.1"
                     /translation="MATDALSPSAVPALVETAGLTKRYGDLVAVDHLDLTVRAGEVYG
                     LLGPNGAGKTTTIMMLLGLTEPSEGTARVLGLDPARRALDVKRRVGYLPDAVGFYGRL
                     TGRENLRYTAALNGLPPDDTEDLIEEALEHVGLADAADRPTGGYSRGMLQRLGIADAL
                     LKDPAILILDEPTTSIDPAGVTELLALIRGLVADRGVGVLLSSHLLAQVERLCDRVGI
                     FWRGRLLAEGTVAEVAAQAGEPGIDLDEAYGRIVRRAGAAETTDGRAA"
     gene            complement(5753..>6430)
                     /locus_tag="VFV59_02920"
     CDS             complement(5753..>6430)
                     /locus_tag="VFV59_02920"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEU5324812.1"
                     /translation="FSVNLENQTPQQLTLALNATAPQGWTVEVRPEGEARAASVTIDA
                     RGSKRLTVQATPPADVASGQYPLRVDAVSGDLGATLDLAAEVTGKVEMQFTTADQRLN
                     TTATAGGTQDVDVVVANTGTSPITAITLSGSGPSDWEVTFDPESIDEIAPGETATATA
                     HIVPSQNAVAGDYVVTLKAGAEGANESMDIRVTVETPPIWTIVGIALIVLTLGGLVWV
                     FRRYGRR"
BASE COUNT          928 a         2316 c         2289 g          897 t
ORIGIN      
        1 cggcgggcct cggtgcatcg gctcagtggt gggtcaggcg ggcgatgggg ccgtgcccgg
       61 ccgcggacgc ttgccctcga cctcgggctc gacgtcctcc gtgacgccct cgccgccggt
      121 cgcctcgacg ccgatcttcc gcggcgtcgg ggtcccggtg cgcggtggca ggacgccgtc
      181 gaagagcgcc tcgaactcct cgtgctcgat cgtctccttc tcgaccagga gcgacgccag
      241 ccgatccagc acctcgcggt gggacgtcag cgcctccttg gcccgggcga aggcgttgtc
      301 gatgatcgag cgcacctcgg cgtcgatctt ggcggcgacc tcgtccgagt agttgcgctg
      361 ctcgctgatc tcgcgaccga ggaagaccat ctcgtccttc ttgccgaagg cgagcggccc
      421 gagcttctcg ctcatgccgt actgggtgac catcgcgcgc gccaggccgg tggccttctc
      481 gatgtcgttc gagctgccgg tcgtcgtgtc gccgaagacg atcgtctccg ccacattgcc
      541 gcccagcatg ccggcgatct tgtcctcgaa ctccgacttg gagtggaggt atcggtcctc
      601 ggacggcagg ctcatggtgt acccgagcgc catgccgcgg ctgacgatcg tgaccttcgt
      661 caccggatcg cacttgggga gcacgcgctg cacgacggcg tgaccgccct cgtggtaggc
      721 gatgatctcc ttctcctcct cggtgatgat ccggctgcgc cgctcgggcc cggcgacgac
      781 gcgatcgatc gcctcgttga actccgccat gcccaccgac ttcttgttgc ggcgagcggc
      841 caggatcgcc gcctcgttga cgaggttcgc caggtcggct ccgctgaagc ccggcgaccg
      901 gcgcgcgagc gcctcgatat cgaccgtctt gtcgagcggc ttgcccttga tgtggacgtt
      961 gaggatctcc ttgcggccct tgatgtcggg ccggtcgagg accacctggc ggtcgaatcg
     1021 gcccgggcgc aggagcgccg ggtcgaggac gtccgggcgg ttggtggcgg ccaccacgat
     1081 cacgttcgtg ttcgtgtcga agccgtccat ctcgaccagg atctggttga gcgtctgctc
     1141 gcgctcgtcg tgcgagccgc ccaggccggc accgcgctgg cggccgaccg cgtcgatctc
     1201 gtcgacgaag acgatgcacg gcgagttgcg cttggcctgc tcgaagaggt cgcgcacgcg
     1261 ggatgcgccc acgccgacga acatctcgac gaactcggag ccggagatgc tgaagaacgg
     1321 cacccccgcc tcgccggcga cggcgcgggc cagcagcgtc ttgccggtac cgggcgggcc
     1381 gaccagcagc acgccgcgcg ggatccgcgc gccgagcgag ttgaacttct cggggtactt
     1441 gaggaactcg accacctcgg tcagctcctg cttggcctcg tcgaccccgg ccacgtcgtt
     1501 gaacgtgacg acggtcttgt tgcccaggaa catgcgcgcg cggctcttgc cgaagctcat
     1561 ggcctggttg ttggtgccct gggcctgccg catcatgaag aagatgaagc cgccgatcag
     1621 gagcaccggg agaagcgcgg tgatgaggag cgtgaggatc ccgccggcgg cgctctcctc
     1681 ctggacggtg aacgtctcgc agttgaagtc acccgaggcg gcgtccgcgc agccgagatc
     1741 ggcatacacg ttcgtcagct ccgatggggc aaccgccgtt cgctcctggc cgctgttcag
     1801 gaccgcgttc agccgctggc cctgctgggt gatggacttg atgtttccgt cgcgggcatc
     1861 ggcgagcatc tcgccgtagc catagtcggc gctctggctg ccgtcgccca tgaacgtcca
     1921 tagcacggcc agtccgaaga cggccaggac gacgagcacg acgctgttgc gaacgatgcg
     1981 gctggtcaag gtgggtctac tttcgcctcc gtggtgacga cgccggccag cggccgccgc
     2041 cgaccctcga tatgcacatc gtgggccgaa tcagtatacg gtgcgctccg ggacccgaac
     2101 aaacgtccga atccccaccg caccactcat acgcgtggcg tgtccggctg gctcaccggt
     2161 cgtcgagcac ggtgatccgc cgctgcctga ccgatgcgcg ccgtccaccg cccagctcga
     2221 tcgtgaggcc gccgcggggc ccggtcgccg cctccagcag cgcctcgacc cgctcggccg
     2281 acgccgcggg atcgccgatg gcgaggcgca gcacgcggcg tcccagggcc gcgggcgggg
     2341 ggtcgcgcca gtcgatggat ccgtccgccg cgcgccgccg atccagctcg gccgccgcga
     2401 cctcgtcgag cagggcgtcg tcgtccgccg ccaggcgcgc gaactgaccg atgcgatcca
     2461 ccgcgcccgg gcgcagctcc tcgaggagcg gcagcagctc ggcgcgcacc cggttgcgca
     2521 ggtaccgggc ctcgtcgttc gagggatcga cccggtacgt gatcccggcg ccgtccagca
     2581 actgccggag cgtcgcgcga cgctggtcca gcaacggccg aacgatgcgt ccgcgcctcg
     2641 ccggcatgcc gcgggcaccc gccagcccgg agccccgaag caggttcagg aggacagtct
     2701 cggtggcatc gtcggcggtg tgtgcggtcg cgatccaggc gtagtcgccg gccagccgcg
     2761 ccagaaaccg atatcgcgcc tcgcgccccg cgtcctcgac cgagcggccc tcggcggccg
     2821 cgaggcgcgc gacgtccacc cggcgcgcct cggagcgaag tcccaggtcg gcggcggcat
     2881 cgttcacgaa cgtggcgtcg tcggcgctcg cggggcgcag cgcatggtcg aggtgcgcaa
     2941 ccgtgaggcg ccaggcgacc gcttcggtcc gacatgcgtg ggtggcgcca tggagcaggg
     3001 ccatcgagtc ggaaccgccg gagacggcga ggatcaggtg ggcgtccgcg gggacgccga
     3061 gggcccgggc gccgcgcacg acggcaccgg ccagggcgtc gaggggcgtg ggattggtag
     3121 cggagggcgg attcgaacca ccgaccaagg gcttatgagt cccctgctct accactgagc
     3181 tactccgcca cgcggtccga cgattctagc gtgagcctcc gaatcgctcc agttcgccac
     3241 gcgccaccgt ggtcgaggtc gtcccccgcg gacaggtacg cactatctgc cacgcaggaa
     3301 cagcacctcc gcgccgatgg gcacgaagcc ggccgccagg aaggcccgca gcgacgcggc
     3361 gttcccgggc gcggcctgcg cgaagacgcg ttcgccggac ggcaccaggg cgagcgcggc
     3421 agcgatcatc cgacgaccga tgccgcggcc gcgggccgaa ggatccagct cgaccgagac
     3481 ctcgaggcga ccagcaaggc cgcgcccgag ggtgacgaca ccgcgctcct ccgcatcggt
     3541 ccaggcccgc acctccgatc ggtagcgctc cgcacgggcc acccgggcgt ggtcggcggc
     3601 cgagaccgac cgcagctcca ccgacggcga tccgcggccc gccgcgacga gcacgacgtc
     3661 gaggctccct ggctccgccc gcagctgctc gcccaaccag gccaggaacg acgcccccat
     3721 cggcgcaccg aggtcggcgc cgccgagccg ctgacggacc gcctctgggt cgacggcggc
     3781 ggccaccacg tggtgcgcgg tgaaggccac gaccgcatcg gcgtgaccgg gcggcgacgg
     3841 gaggacggcc accgacccgt ccgccggagg cggatggccc cgtgctgcgc gctcgagcag
     3901 gtcgcgaagc gggtgccgcg catgcatcgg ctccatggag gtggagccta cgcgcgaacc
     3961 tcctgtcgca ggaagacgat gtagctcgcc acgaagcaca ggaccatcgc ggccagcagc
     4021 ccgacgacct ggggccacac gatgagcagg ctctgctgca gcgacagctg cgtcgggatc
     4081 tgctggctca gctgaatcgc ctccgcgagc ccgggcacga ccaccgcggt ggcgccgggg
     4141 ttcagcagcg ccagcgtcgc ctgcgcgtag accgtcccgg ggttcacgat gctcaactgc
     4201 tgctgcagct gcgcgtggcg cagcgccgcc tcggtgccga ccgagccgct gatcgggtcg
     4261 aggaagcccg cgatgagctg ggccagcagc acgccgaaga ggctcagcgc cagccagagt
     4321 ccgatcgcga tcagcgccga tgtcgccgct cgccggctga gcacggagca gagcatggcg
     4381 atcgccagcc acaggccgat gtagaggatg gtcacgacga cccaggccag catgcgcgcg
     4441 acctcgccca cgctcggcac gatcccgagc cgcacgatgc cgaggcccgc gaccaggagc
     4501 gccatgctca cgacggcgaa ggcgatggcg gccaggccag cggcgaactt gccgttgatc
     4561 aggtcgtcgc ggtggatcgg gtgcgagagc aggcgcgcca gcgtgccctg cgcccgctcg
     4621 ccgttgatcg cgtcgaagcc gaaggcgatg ccgagcagtg gcgccacgaa cccgaccagg
     4681 ccgacgaacg agggcaccgg gtccgcgccg agcgtgaaga gcgcgaggaa cagggccgcc
     4741 gcgccgctcg cctgctccgc cgcgtcgcgg atggcgccgg aggtggcgta caccggggcg
     4801 agcgcggcca gtcccaggat gaccagcagg acggcgaagc ggaggctggt cagcgtctcg
     4861 gcgagctcct tgccggcgac gacgagccag ccggggccac gacgccgcag ccactcggtg
     4921 tcggcagctg cggcaacgcg aacggcgtca ggctgcgcgg ccatcggtcg tttccgcagc
     4981 gccggcgcgg cgcacgatgc gcccgtacgc ctcgtccaaa tcgatccccg gctccccggc
     5041 ctgcgccgcg acctcggcca cggtgccctc cgcaagcagc cgtccgcgcc agaagatgcc
     5101 gacccggtcg cacagccgct ccacctgcgc gagcaggtgg ctcgacagga gcacgcccac
     5161 gccacggtcc gccaccaacc cgcggatgag cgccaggagc tcggtgacgc cggccgggtc
     5221 gatcgaggtg gtcggctcgt ccaggatcag gatggcgggg tccttgagca gcgcatccgc
     5281 gatgcccagg cgttgcagca tgccgcgcga gtacccgccg gtcggccggt cagcggcgtc
     5341 ggccagcccg acgtgctcca gtgcctcctc gatgaggtcc tcggtgtcat ccgggggcag
     5401 gccgttgagc gccgcggtgt agcgaaggtt ctcgcgcccg gtgagccgtc cgtagaagcc
     5461 gacagcatcg ggcaggtagc cgacccggcg cttcacgtcg agggcgcgcc gcgccggatc
     5521 caggcccagg acccgggcgg tgccctcgct cggctcggtg aggcccagga gcatcatgat
     5581 cgtcgtcgtc ttgcccgccc cgttcggacc cagcaggccg tagacctcgc cggcgcggac
     5641 cgtcaggtcg aggtgatcga ccgcgacgag gtcgccgtag cgcttggtca gcccggcggt
     5701 ctcgaccagg gccggcaccg cgctgggcga gagggcgtcc gtggccatgg gctcaccgcc
     5761 gaccataacg gcggaacacc cagaccaggc cgcccagcgt gagcacgatc agcgcgatcc
     5821 ccacgatggt ccagatgggc ggcgtctcga cggtcacgcg gatgtccatc gattcgttcg
     5881 cgccctccgc cccggccttg agcgtcacga cgtaatcgcc ggccaccgcg ttctgcgacg
     5941 gcacgatgtg cgcggtggcg gtcgcggtct cgccgggcgc gatctcgtcg atcgactccg
     6001 ggtcgaaggt gacctcccag tccgacggcc cgctgccgct cagcgtgatg gcggtgatgg
     6061 gcgacgtccc ggtgttggcg accacgacgt cgacgtcctg ggtgccgccg gccgtcgcgg
     6121 tggtgttgag ccgctgatcc gccgtcgtga actgcatctc caccttgccg gtgacctccg
     6181 ccgccaggtc cagggtggcg ccgaggtcgc ccgagacggc gtcgacgcgc agcgggtact
     6241 ggccggaggc cacgtcggcg ggcggcgtgg cctgcaccgt cagccgcttc gacccccgcg
     6301 cgtcgatggt cacgctggcg gcacgcgcct cgccctccgg ccggacctcg accgtccagc
     6361 cctgcggcgc ggtggcgttg agggccaggg tcagctgctg cggcgtctgg ttctcgaggt
     6421 tgacgctgaa
//
LOCUS       DASTYP010000059         5503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1278893, whole genome shotgun sequence.
ACCESSION   DASTYP010000059 DASTYP010000000
VERSION     DASTYP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278893"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFV59_02925"
     CDS             <1..567
                     /locus_tag="VFV59_02925"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5324813.1"
                     /translation="LEQMSTDADGRVRMEFIVPTRGLIGYRGQLLSDTRGTALMHQIG
                     EGYGPWAGEVTHRTTGVLVADRPGESRAYALSSLVERSELFIGPSTEVYEGMIIGENS
                     RPGDMDVNPTKSKKLSAMRTQATDGDYRIFTPPRVLTLEAAIEFIAADELVEVTPSAI
                     RLRKRILSAHDRKRALGAAFKAAQGVGD"
     gene            complement(1019..1489)
                     /locus_tag="VFV59_02930"
     CDS             complement(1019..1489)
                     /locus_tag="VFV59_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324814.1"
                     /translation="MIEWYPWIVLLHVIGAFGFVFAHGASAFAAFRIRTDPRPESVAS
                     MLALSGGALTVMYPSLLLLLVGGIWAAFAGDLWGHWWVWAALGLLIGVAAAMYAVGTR
                     YYVALRQRLAVEGGVPEGDPDFAAVLDSRRPEMLAAIGGTGLLLILWLMVVKPG"
     gene            complement(1486..1896)
                     /locus_tag="VFV59_02935"
     CDS             complement(1486..1896)
                     /locus_tag="VFV59_02935"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEU5324815.1"
                     /translation="MVARYAYVTLAWLFLACVAVQVFLAGLGVFAGSQNFATHRDFGY
                     TFGFLTVGMLIAAGVGRLGRRWIWFSALLIFLFALQSVFVGVRDTLPAVAALHPVNAL
                     AIFWVAQHVARGALSLVRNAAEAPAAAERLGVRP"
     gene            complement(1992..2639)
                     /locus_tag="VFV59_02940"
     CDS             complement(1992..2639)
                     /locus_tag="VFV59_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5324816.1"
                     /translation="MIRVLLVDDHPVYRDGVRLNLEGWEEVEVIGEAGDGDAAIEAAA
                     RLRPNVILMDVQMPGRSGVDATREIVARDPGVAIVVLTMFEDDELVAAAMRAGARGYL
                     LKDAGREELRRAVAAAASGQAIFSASVAHRLTSLVGASPAPPPFPELTPRERDVLELM
                     AQGRLNADIAQRLGLSEKTVRNQVSVIFSKLEVLGRPEAIVKAREAGMGAEGPRP"
     gene            complement(2636..4897)
                     /locus_tag="VFV59_02945"
     CDS             complement(2636..4897)
                     /locus_tag="VFV59_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5324817.1"
                     /translation="MGRPWRLDLRTSLVAFSGVAMLAVAGASLAYRAAGPSDGSHAGY
                     VTSAVTRDGVEVTRLPGQPTALEDGDRVEAIDGVPLESWLRRGPSDRPSVDRGDRATY
                     DVARDGRAAQVAVRIGQYPLGRVLLDSWGTVIFLLVTLGVTAYVFVRRPSEPATRPLL
                     LLGTGLVGSTIPWMLGLQPLDLVTGTGLWVWFIGSFVVYSVFWSASLHFSLVFPRRIG
                     LVRRIPAVTWLVYAVPLALLLAWALVGVAASGSAMATLGSMTGLQLLMVLGTCVSLVA
                     AQVVQYRTAGEGSAREQTRWLAWGGISATVLVVAFWFGPELVTGRPTLPWSAVGLPGL
                     LFTGAIAVAVLRHRLFDIDVVINRTLVYGTLTASVAATYFVGVLVLRSVMGTDANFAV
                     SLLATGAAALLALPIRDALQRGVNRLMYGDRDDPYRAITRLGERLGSSLTTEEVLPTV
                     VATVATALRLPYVAIELRQGDVVSVAASTGSPPADADAITRLPLVDRGETIGDLAVAA
                     RGPGERFSRADHALLAGLAQEAGRAARSVRLAAELDRSRRQLVAAREEERRRLRRDLH
                     DGLGPTLAAARLKVQAVRAGAADPSVLDQLDGELAGVLGDVRRISRELRPPALDELGL
                     MPALRAHAARFAVDGELTVQVDGPGDLPALPAAVESAAYLIAMEALTNVRRHSGAVHC
                     RVRVRLADALELEIEDDGAGLAAEPEPGVGLASMRERAAEVGGSVTIGRGHDAGTLVL
                     ARLPLTAPSGGAA"
     gene            5214..>5503
                     /locus_tag="VFV59_02950"
     CDS             5214..>5503
                     /locus_tag="VFV59_02950"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5324818.1"
                     /translation="MPQPSAERRLVSVLFADLVGFTPLAESRDAEAVRELLSRYFDTA
                     TQIIGSYGGTVEKFIGDAVMAVWGAPTAHEDDAERAVRSALDLVSAVQSMGA"
BASE COUNT          868 a         2011 c         1984 g          640 t
ORIGIN      
        1 ctggagcaga tgagtaccga tgcggacggg cgcgtccgca tggagttcat cgtgcccacg
       61 cggggactca tcgggtaccg cgggcagctg ttgagcgaca cgcgggggac ggcgctgatg
      121 caccagatcg gtgagggcta cgggccgtgg gcgggtgagg tcacgcatcg gaccaccggc
      181 gtgctggtgg ccgaccgccc gggcgagtcg cgcgcgtacg cgctgtcgtc gctggtggag
      241 cgttcggagc tgttcatcgg tccgtccacc gaggtgtacg aggggatgat catcggcgag
      301 aacagccgcc ccggcgacat ggacgtcaac ccgaccaagt ccaagaagct ctcggccatg
      361 cgcacccagg cgaccgatgg cgactaccgc atcttcaccc cgccgcgcgt cctcacgctc
      421 gaggcggcca tcgagttcat tgcggccgat gagctggtcg aggtgacgcc gtcggccatc
      481 cggctgcgga agcggatcct ctccgcgcac gaccgcaagc gggcgctcgg agcggcgttc
      541 aaggcggcgc agggggttgg cgactgactg acgctccggc tgcgtcgtgg gtggttgcgt
      601 gtcgcgcaac tatccggggc gcgcgtgacg actcagtcgt tgagcggcgc cgcgttgcgt
      661 gtcgcgcaac tatccgggac gcgcgtgacg attcggtgcg ttcggcggcc ctgcgttgcg
      721 tgtggcacaa atccagggcg acggagccag catgcgcggg ggctccgtct cgggtagtcg
      781 cgccccacgc aaccgccggc aggtgcgcgc gcggcaagca gaggcagacg acccgcgacc
      841 cgagccgccc gcttgcccac cccgcaagca acgcccgagt tgcggccctc gcggcgctcc
      901 cgacggccgt ggcttgcgcc accggcaact caccgccagg ttggcggcca actcggcagt
      961 tacttgccgc tgccgcaagc aggacgccgc gcgcgcggcg gccgtcggcg cacccgcgtc
     1021 accccggctt gaccaccatg agccacagga tgaggagcag cccggtaccg ccgatggcgg
     1081 cgagcatctc cggccgccgc gagtcgagca cggccgcaaa gtccgggtcg ccctcgggaa
     1141 cgcctccctc cacggccaac cgctgccgga gcgcgacgta gtagcgcgtc ccgaccgcgt
     1201 acatcgcggc ggccacgccg atcagcagcc ccaacgccgc ccatacccac cagtggcccc
     1261 acaggtcgcc ggcgaacgcg gcccaaatgc cgccgacgag caggagcagc agcgacgggt
     1321 acatgaccgt cagggcgcca ccggagagag cgagcatcga ggcgaccgac tcgggtcgag
     1381 gatcggtgcg gatgcggaag gccgcaaagg ccgacgcacc gtgggcgaag acgaagccga
     1441 aggcgccgat gacatggagc agcacgatcc acggatacca ctcgatcacg gtcgcacccc
     1501 caggcgctcg gcggcagccg gtgcctccgc agcgttgcgc accagcgaca gcgccccgcg
     1561 cgcgacgtgc tgtgcgaccc agaagatggc cagcgcgttg accgggtgca gggcggcgac
     1621 ggccggcagg gtgtcccgca ccccgacgaa cacggactgc agcgcgaaca ggaagatcag
     1681 cagggcggag aaccagatcc atcggcgacc caggcggccc acacctgcgg caatgagcat
     1741 gccgacggtc aggaagccga aggtgtagcc gaagtcgcga tgcgtggcga agttctgcga
     1801 cccggcgaac acgccgagcc cggcgaggaa cacctgcacg gcgacgcagg cgaggaagag
     1861 ccaggcgagg gtgacgtagg cgtatcgggc gaccatggac gcgactcctg agctgagcga
     1921 tggccgcaat ctgcgccgga cgctgtcccg ggcgcatcgg tcaagcgtcc cggacagccg
     1981 ggaggcgcgc ctcagggccg tggaccttcg gcgcccatgc cggcctcgcg cgccttgacg
     2041 atggcctccg gccggccgag gacctcgagc ttggagaaga tgacgctgac ctggttgcgc
     2101 accgtcttct cgctcagtcc cagccgctgc gcgatgtcgg cgttgaggcg cccctgggcc
     2161 atcagctcca ggacgtcccg ctcgcgcggg gtcagctcgg ggaacggcgg tggtgccggg
     2221 ctcgcgccca ccagcgaggt gagccgatgc gccaccgagg cgctgaagat cgcctggccg
     2281 ctggccgcag cggcaacggc ccggcgcagc tcctcgcgcc cggcatcctt cagcaggtag
     2341 ccgcgggctc cggcgcgcat cgcggccgcg acgagctcgt cgtcctcgaa catcgtcagc
     2401 accacgatcg cgacgcccgg gtcgcgcgcc acgatctcgc gggtcgcgtc gacgccggac
     2461 cgcccgggca tctgcacgtc catcaggatc acgttcgggc gcagccgggc cgccgcctcg
     2521 atggccgcgt caccgtcacc ggcctcgccg atcacctcga cctcctccca accctcgagg
     2581 ttgagtcgga caccgtcgcg gtagacggga tggtcgtcga cgagcaggac gcggatcacg
     2641 ccgcgccccc gctcggcgcg gtcagcggga gtcgggcgag gaccagggtg ccggcgtcgt
     2701 ggccgcgccc gatggtgacc gacccgccga cctcggccgc ccgttcgcgc atcgaggcca
     2761 gcccgacgcc gggctccggc tccgcggcca ggccagcccc gtcgtcctcg atctccagct
     2821 cgagggcatc ggccaggcgg acccggacgc ggcagtggac ggcgccgctg tggcgccgaa
     2881 cgttggtcag ggcctccatg gcgatcaggt aggcggccga ctcgacggcg gccggcaggg
     2941 cggggaggtc gccgggacca tcgacctgca cggtcagctc accgtcgacc gcgaagcgcg
     3001 ccgcgtgggc gcgcagcgcc ggcatgagcc caagctcgtc gagcgccggc ggacgcagct
     3061 cgcgagagat gcgacggacg tcgcccagga cgccggcgag ctcgccgtcc agctggtcca
     3121 gcacggacgg atcggcggcc cccgcgcgca ccgcctggac cttgagccgc gcggccgcca
     3181 gcgtcggccc gagtccgtcg tgcaggtcgc ggcgcagccg gcgacgctcc tcctcgcgcg
     3241 ccgcgacgag ctgccgccga gagcggtcga gctcggccgc caggcggacc gatcgcgccg
     3301 cccgccccgc ctcctgcgcc aggccggcca gcagggcgtg gtcggcgcgg gagaagcgct
     3361 cgcccgggcc tcgggctgcg accgccaggt cgccgatcgt ctccccgcga tcgacgagcg
     3421 gcagccgcgt gatcgcatcc gcatcggcgg gcggacttcc ggtcgacgcc gccacgctca
     3481 cgacatcgcc ctggcgcagc tcgatggcca cgtacggcag gcgcagggcg gtggcgacgg
     3541 tggcgacgac agtgggcagc acctcctcgg tggtcagcga cgatccgagc cgctcaccca
     3601 gccgcgtgat cgcgcggtac ggatcatccc gatcgccgta catcaggcgg ttgacgcccc
     3661 gctgcagcgc gtcccggatc ggcagggcga ggagggcggc cgctcccgtg gcgagcagcg
     3721 acacggcgaa gttcgcatcg gtgcccatga cggaacgaag gaccagcacg ccaacgaagt
     3781 aggtggccgc caccgatgcc gtcagcgttc cgtacaccag cgtgcggttg atgacgacgt
     3841 cgatgtcgaa cagccgatgc cgcagaaccg cgacggcgat tgcgcccgtg aacaacagtc
     3901 ccggcaggcc gaccgcgctc cacggcaggg tgggccggcc ggtgacgagc tccgggccga
     3961 accagaacgc gaccaccagc acggtggccg agatgccgcc ccaggccagc catcgagtct
     4021 gctcgcgcgc cgatccctcg cccgcggtgc ggtactggac cacctgggcg gccacgaggc
     4081 tcacgcacgt gccgagcacc ataagcagct ggaggccggt catgctgccc agcgtcgcca
     4141 tcgcgctgcc gctggccgca acgccgacga gcgcccacgc caggagcagc gccaaaggca
     4201 ccgcgtacac cagccacgtc accgctggga tccgccgcac caggccgatc cggcgcggga
     4261 acaccaggct gaagtgcagc gaggcgctcc agaagacgct gtacacgacg aaggagccga
     4321 tgaaccagac ccacaagccg gtgcccgtga ccagatccaa cggctggagg ccgagcatcc
     4381 aggggatcgt gctgcccacc agcccggtgc cgagcaggag cagcggtcgc gtcgccggct
     4441 ccgatggacg gcggacgaag acgtaggccg tcaccccgag cgtcacgagc aggaagatca
     4501 cggttcccca ggagtccagg aggacgcgcc ccagcgggta ctgaccgatg cggaccgcca
     4561 cctgtgcagc ccggccgtcg cgcgcgacgt cgtacgttgc ccggtcgcca cggtcgacgc
     4621 tcggccggtc cgacggccct cgcctgagcc acgactccag cggtacgcca tcgatggcct
     4681 cgacccggtc cccgtcctcg agggccgtcg gctggccggg caggcgcgtg acctcgacac
     4741 cgtcccgcgt cacggcgctc gtcacgtagc cggcgtggga gccatcggac ggcccggccg
     4801 cccggtaggc cagggacgcc ccggcgacgg ccagcatcgc cacgcccgag aacgccacga
     4861 gcgaggtgcg caggtcgagg cgccacggcc gacccatggc agcgcagcct acgaggcggg
     4921 cgccgcgttg ggaagggcgg gccgccgcgg atgaggcgtc gggcacgttg accgccgtcc
     4981 cgcgcgcagc cgatactccg caacgccacg aaggagagcc gatggagtgc cgggcatgcg
     5041 ggacgccgaa cgaggccgac cgcaagttct gcggcgaatg cggagccgct ctcgcggccg
     5101 cgtgccccag ctgcggcgcg tcgaacgcgc ccggcgtcaa gttctgcggc caatgcggga
     5161 ctgatctccg gctctccgcc tcctcggctc ccgcggccga gtcgacggcc gccgtgcccc
     5221 agcccagcgc ggagcggcgt ctcgtctcgg tgctcttcgc ggacctcgtc ggcttcacgc
     5281 ccctcgcgga gtcccgcgac gcggaagcgg tccgcgagct gctgagccgc tacttcgaca
     5341 cggccacgca gatcatcggc tcgtacggcg ggacggtgga gaagttcatc ggtgatgccg
     5401 tgatggccgt gtggggcgcg ccaacggccc acgaggacga cgcggagcga gcggtgcgga
     5461 gcgcgctcga cctcgtctcc gccgtgcagt ccatgggggc gga
//
LOCUS       DASTYP010000060         6006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_259291, whole genome shotgun sequence.
ACCESSION   DASTYP010000060 DASTYP010000000
VERSION     DASTYP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259291"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFV59_02955"
     CDS             complement(<1..296)
                     /locus_tag="VFV59_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5324819.1"
                     /translation="MTGAPRDEALLRVEGLVKEFPGRRSIGDWLARRPAPPVRAVDGI
                     DFELRRGEVLALVGESGCGKTTTGNLLLGLLAPTGGRVMLDGQEVGGLSGRDLRR"
     gene            complement(293..2224)
                     /locus_tag="VFV59_02960"
     CDS             complement(293..2224)
                     /locus_tag="VFV59_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEU5324820.1"
                     /translation="MSALLEATASPTVVAPQRGAWRLLLRQRMGVIGAVMLLLAVVVA
                     IAAPLLAPYDPYANVRVNILDIYQAPSTTHLLGTDDGGKDVLSSLIFGARVSLVVGFS
                     AAAIALVIGGLVGIVAGYRGGWIGSLLMRITDFFLVIPDLALQIVLVAIIGPSLGTII
                     LVIGALGWTTTARIVRSQTLTVRERKYVMRARAVGAGDLHILREHILPAVLPLMLANM
                     VLVISLAILSESTLAFLGLGDPTVISWGQMLNFAFDRGAVSAGAWWALLPPGFAIVWV
                     VLATTLLGTALENALNPRLERHHLEAPGSDVARDRRPTPLRTLPDDPILRVRDLTIEF
                     DTPAGPLRAVDGVSFDLRRGETLGLVGESGCGKTTTVLGLLRLLPPGGRVVNGSVWFD
                     GEDLLGLDGDALRAFRWTRLSLVFQGAMNALNPVRTIGDQIAEAVRLHAPTTTKAEAR
                     RRAEDLLERVGIGRRRANEYPHTYSGGMRQRAMIALALACDPDVIVADEPTTALDVMI
                     QAQILELLAGLSRDLGMGIILVTHDLGVVAQVCDRVVVMYGGEVAEENEAAALYRAPR
                     HPYTQQLLASFPDLAHPDRPLRGIPGSPPRLDAMPPGCRFAPRCPHAFDRCVLERPPE
                     YGTDGGRAACFLLDPGVDR"
     gene            complement(2229..3236)
                     /locus_tag="VFV59_02965"
     CDS             complement(2229..3236)
                     /locus_tag="VFV59_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324821.1"
                     /translation="MSGRGGYALRKVGWALLTLFVVITFNFLLFRVLPGDPAKSGLRD
                     PRLNPAAVEALRERFGLDKPVFLNLDGGNPLDTQFTAYLGALSRGDLGQSYAFRDQSV
                     ADLIGQALGNTLWLVVPSQFLSILLGALLGLVAAWRRGRALDVGALSFSLFMWSLPTF
                     FLGIILLVVGANWFGLPTAGRITIGADYANAWELAWDVGRHLLLPTLTLTLVVLGEYM
                     LIMRSSVLDVFSEDYILTARAKGLSTFRVIRDHALRNAMLPMVTLIALNLGFTVSGAI
                     QVESVYSWPGIGLLTVNAVADRDYPVLQGAFLLIAVSVVVANLVAELVYGWLDPRVSE
                     G"
     gene            complement(3339..5075)
                     /locus_tag="VFV59_02970"
     CDS             complement(3339..5075)
                     /locus_tag="VFV59_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5324822.1"
                     /translation="MRRHATLISLLGILALVGAACTAVQQESPSAGDSSGGESAAPTG
                     GTASGGTVRIAMGGYPDSLNPGNGVLSEAYTFYELMYDTPISVNSAGEFVPELASEWS
                     VSDDGLTWTMTIRDGVAFHDGEPLTAEDVAYSMNLYRDTEDFPFLPSYVADFTSIEAT
                     DDTTVVLTTEEPMAAFEANMAFMYVLPKHIWENESDPVGFENAEMIGSGPFKLVEASQ
                     GEFVELEANADYWNTPPNVDGVIFQTIDNADARVTALTTGEVDAITEFPATAASALQN
                     AEGVTVHIADVAAGGAFRDVFFNVVSDEDCPQDDPETTDADETGVCSGHPALKDINVR
                     QALAMATDKQQIIDVATLGTSSPGLSLVPPGLGDYYADDVEDYAFDTDAANELLDQAG
                     YEDTDGDGIRECLPDQGCDDLTFRFNYADDIDTAPREAELLQGMWSEIGVAIEIQGLD
                     PDTLTSVCCPSFDYDVMLWGWGSDPDPAFLMGVALCDEIPSGFSETGYCNPEYDDLYT
                     AQSTEQDHDTRVGMIHDMQRILVEDVPYIIPYYQQELEAWREDRFTGWLAEDPTLGLD
                     DPSSLLSLRPSE"
     gene            5249..>6006
                     /locus_tag="VFV59_02975"
     CDS             5249..>6006
                     /locus_tag="VFV59_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324823.1"
                     /translation="MTDATPSALVRAQVALESFDLAPDASFVVYGLRRVVRGEYESQL
                     WMRPLRGGRARQLTRGRVRDTGPAVSPDGRQLAFVRSKVGSADPGQAWILPLDGGEPW
                     QLTRLKHGVGSVLWSPDGERLALVAQAGDHRFVVGEEKPGRTPIARRITRLDFRDDES
                     GHVVRRSHLWIVPAREGAQPRQLTSGDFDVAHPAWHPDGRSIAFAADTGPDATINPRL
                     QIRTVSLADAPPGNAKVRELASLAGDADYPAWSP"
BASE COUNT          919 a         2129 c         2129 g          829 t
ORIGIN      
        1 cgacggaggt cccggcccga cagcccgccg acctcctgcc cgtcgagcat cacgcgcccg
       61 ccggtcggcg ccagcagacc gaggagcagg ttgcccgtgg tcgtcttgcc gcaccccgac
      121 tcgccgacga gcgcgaggac ctcgccgcgc cgcagctcga agtcgatgcc gtccacggca
      181 cgtaccggcg gtgccgggcg gcgcgccagc cagtccccga tggaccggcg ccccgggaac
      241 tccttcacca ggccctcgac ccgcagcagc gcctcgtcgc gtggcgcccc ggtcaccggt
      301 ccaccccggg gtccaggagg aagcaggcgg cccgaccacc atccgtgccg tactccgggg
      361 ggcgctccag gacgcatcgg tcgaaagcgt gcggacagcg cggggcgaac cggcagccgg
      421 gtggcatggc gtccaggcgt ggcggcgatc ccgggatgcc gcgcagcggc cgatcgggat
      481 gggccaggtc cgggaaggag gccagcagct gctgggtgta gggatggcgc ggcgcgcggt
      541 acagcgccgc cgcctcgttc tcctcagcca cctcgccgcc gtacatgacc accacccggt
      601 cgcagacctg ggccacgacg ccgagatcgt gggtcaccag gatgatcccc atccccaggt
      661 cccgcgagag gccggcgagg agctcgagga tctgggcctg gatcatcacg tccagcgcgg
      721 tggtcggctc gtccgccacg atgacgtcgg ggtcgcaggc gagcgccagc gcgatcatgg
      781 cgcgctggcg catgccaccg gagtacgtgt gcggatactc gttcgcgcgg cgccggccga
      841 tgcccacgcg ttcgagcaga tcctcggcac gcctccgcgc ctcggccttc gtggtggtcg
      901 gcgcatgcag ccgcacggcc tccgcgatct ggtcgccgat ggtccggacg gggttgagcg
      961 cgttcatcgc cccctggaag acgagcgaca ggcgcgtcca gcggaaggcg cgcagggcgt
     1021 cgccgtccag ccccagcaga tcctcgccgt cgaaccacac cgaaccgttc acgacgcgcc
     1081 cgcccggcgg caggagccgc agcaggccga gcacggtcgt cgtcttcccg cacccggact
     1141 cgccgaccag gcccagggtc tcgccgcgtc ggaggtcgaa gctgacgccg tcgacggcgc
     1201 gcagcggacc ggccggggtg tcgaactcga tcgtcaggtc acggacgcgc aggatcggat
     1261 cgtccggcag cgttcgcagc ggcgtcgggc ggcggtcccg cgccacgtcg gagccgggcg
     1321 cctccaggtg atgccgctcg agccgagggt tcagcgcgtt ctcgagcgcc gtgcccagca
     1381 gcgtggtcgc cagcaccacc cacacgatgg caaagcccgg cggcagcagc gcccaccacg
     1441 cgccggccga gacggcaccg cggtcgaagg cgaagttgag catctgcccc cagctgatga
     1501 ccgtcgggtc gccaaggccc aggaaggcga gggtggactc cgacaggatc gccaggctga
     1561 tgaccaggac catgttggcg agcatcagcg gcaggacggc gggcaggatg tgctcacgca
     1621 ggatgtgaag gtcaccggcc ccgaccgccc gggcccgcat cacgtacttg cgctcgcgca
     1681 cggtgagcgt ctgcgaccgc acgatccggg cggtcgtcgt ccagcccagc gcaccgatga
     1741 ccaggatgat cgtccccagt gacggaccga tgatcgccac cagcacgatc tgcagcgcca
     1801 ggtccgggat gaccaggaag aagtcggtga tccgcatgag cagcgagccg atccagccgc
     1861 cgcggtagcc ggccacgatc ccgaccaggc caccgatcac cagcgcgatg gccgcggccg
     1921 agaagccgac cacgagcgat acccgcgccc cgaagatcag cgagctgagg acgtccttgc
     1981 cgccgtcgtc ggtgccgagc aggtgcgtgg tggacggcgc ctggtagatg tccaggatgt
     2041 tgactcggac gttggcgtat gggtcgtagg gcgccagcag cggggccgcg atggcgacga
     2101 cgacggccag caacagcatg acggcgccga tgacgcccat ccgctgccgc agcaggaggc
     2161 gccacgcccc ccgttgcggt gcgacgaccg tcggcgacgc ggtcgcctcg agcagggcgc
     2221 tcatcggctc atccctcgct cacccgcggg tccagccaac cgtagacgag ctccgcgacc
     2281 aggttggcga cgaccaccga gacggcgatc aggaggaagg cgccctgcag gaccgggtag
     2341 tcgcggtcgg cgacggcgtt gacggtcagc aggccgatgc ccggccagga gtacaccgac
     2401 tcgacctgga tggcgccgga gacggtgaag cccaggttga gggcgatcag cgtgaccatc
     2461 ggcagcatcg cgttgcgcag ggcgtggtca cggatgacgc ggaaggtgga gaggcccttg
     2521 gcgcgcgcgg tgaggatgta gtcctcgctg aacacgtcca gcacgctgct gcgcatgatc
     2581 agcatgtact cgccgaggac caccagggtc agcgtcagcg tcggcagcag caggtgccgc
     2641 ccgacgtccc acgccagctc ccaggcgttg gcgtagtcgg caccgatggt gatgcgcccc
     2701 gcggtcggca gcccgaacca gttcgcgccg acgaccagca ggatgatccc caggaagaag
     2761 gtcggcagcg accacatgaa gaggctgaac gacagcgcgc cgacgtccag tgcgcggccg
     2821 cgccgccacg cggccaccaa gcccagcagg gcgccgagca ggatggacag gaactggctc
     2881 gggaccacca gccacagggt gttgcccagc gcctggccga tgagatcggc caccgactgg
     2941 tcgcggaacg cgtagctctg ccccaggtcg ccgcgggaga gcgcgccgag gtacgccgtg
     3001 aactgggtgt cgagcgggtt gccgccgtcc aggttgagga agaccggctt gtcgagcccg
     3061 aaccgctcgc gcagcgcttc gacggccgcc gggttgagcc gcgggtcccg caacccggac
     3121 ttggccgggt cacccggcag cacccggaag agcaggaagt tgaaggtgat gacgacgaac
     3181 agcgtcagga gggcccagcc caccttgcgc agggcgtagc cgccgcggcc gctcaccggg
     3241 gagcctcggg ccggtcggcc gtgggtacga agcgcacgac gcgggtcacg cgggtcggac
     3301 cggccgggcg ccgcgatgga cgcccggccg cgatcggtct actcgctcgg tcgaagcgac
     3361 aggagcgacg atggatcgtc gagcccgagg gtcgggtcct cggccagcca gcccgtgaac
     3421 cgatcctcgc gccacgcctc gagctcctgc tggtagtacg ggatgatgta cggcacgtcc
     3481 tccaccagga tccgctgcat gtcgtggatc atcccgaccc gcgtatcgtg gtcctgctcg
     3541 gtggactggg ccgtgtacag gtcgtcgtac tccgggttgc agtagccggt ctcgctgaac
     3601 cccgacggga tctcgtcgca cagcgcgacg cccatcagga acgccggatc cggatccgag
     3661 ccccagcccc acagcatgac gtcgtagtcg aagctcgggc agcagacgct cgtcagcgtg
     3721 tccgggtcca ggccctggat ctcgatcgcc accccgatct ccgaccacat gccctggagc
     3781 agttccgcct cgcgcggcgc ggtgtcgatg tcatcggcgt agttgaagcg gaacgtcagg
     3841 tcgtcgcagc cctggtccgg gaggcactcc cgaatgccgt cgccatcggt gtcctcgtag
     3901 ccggcctggt ccagcagctc gttggccgca tccgtgtcga aggcgtagtc ctcgacgtcg
     3961 tcggcgtagt agtcgccgag gccgggcggc accagtgaca ggccgggcga gctggtgccc
     4021 agcgtggcca cgtcgatgat ctgctgcttg tcggtggcca tggcgagcgc ctgacggacg
     4081 ttgatgtcct tcagagccgg gtgcccgctg cacacgccgg tctcgtcggc gtcggtggtc
     4141 tccgggtcgt cctgcggaca gtcctcgtcc gacaccacgt tgaagaagac gtcccggaag
     4201 gcgccgccgg cagccacgtc ggcgatgtgc accgtcacgc cctccgcgtt ctgcagcgcc
     4261 gacgcggcgg tcgcggggaa ctcggtgatg gcgtcgacct cgccggtggt cagcgcggtg
     4321 acgcgcgcat cggcgttgtc gatggtctgg aagatgacgc cgtcgacgtt gggcggcgtg
     4381 ttccagtagt ccgcgttcgc ctccagctcg acgaactcgc cctgcgacgc ctcgacgagc
     4441 ttgaacgggc cggagccgat catctcggcg ttctcgaagc ccaccgggtc ggactcgttc
     4501 tcccagatgt gcttgggcag gacgtacatg aacgccatgt tggcctcgaa ggccgccatc
     4561 ggctcctcgg tggtgagcac gaccgtcgtg tcgtcggtgg cctcgatgct cgtgaagtcc
     4621 gccacgtacg agggcaggaa cgggaagtcc tcggtgtcgc ggtacaggtt catgctgtag
     4681 gcgacgtcct ccgcggtgag gggctcgccg tcgtggaagg cgaccccgtc gcggatggtc
     4741 atcgtccagg tcaggccgtc atcggagacc gaccactcac tcgccagctc ggggacgaac
     4801 tcacccgccg agttcacgga gatcggcgtg tcgtacatga gctcgtagaa ggtgtaggcc
     4861 tccgacagca ccccgttccc ggggttcagg gagtccggat agccgcccat ggcgatgcgg
     4921 accgtcccgc cgctcgccgt cccgccggtc ggcgcggcgc tctctccccc gctgctgtct
     4981 ccggcgctcg ggctctcctg ctgcaccgcg gtgcaggcgg caccgaccag cgcgaggatg
     5041 ccgagcaggc tgatcaaggt tgcgtggcga cgcacggttc gattcctccg actctgcgtg
     5101 gcgtcgcggc gaggcccggg gcgggatccg gccgcttcga aggccggagg atggcacatt
     5161 ccaccgaatc tgacgaccct ttcctgcacg cccacggatt ccgccatgcg gcgatgtgcc
     5221 gaccgcgcgt gccgcatact ccccagccat gaccgatgcg accccgagtg ctctcgtgcg
     5281 cgcccaggtg gcgctcgaat cgttcgacct cgcgcccgac gcgagcttcg tcgtgtacgg
     5341 gctgcgtcgc gtcgtgcgcg gcgagtacga gtcgcagctc tggatgcgcc cgctgcgtgg
     5401 cggccgcgcg cggcagctga cccgcggacg cgtccgggac accggtcccg ccgtctcccc
     5461 cgatggccgg cagctggcct tcgttcgctc caaggtcggc tccgccgatc cgggccaggc
     5521 gtggatcctc cccctcgatg gcggcgagcc atggcagctg acccgactga agcacggcgt
     5581 cggctcggtc ctgtggagcc ccgacggcga acgcctggcg ctcgtggccc aggcgggcga
     5641 tcaccgcttc gtcgtcggcg aggagaagcc gggccggacg ccgatcgcgc gccgcatcac
     5701 gcggctcgac ttccgcgacg acgagagcgg tcacgtcgtg cgccgatcgc acctctggat
     5761 cgtcccggcg cgcgagggag cgcagcctcg ccagctgacc agcggcgact tcgacgtggc
     5821 gcacccggcg tggcaccccg acggccgctc gatcgccttc gccgccgata ccggccccga
     5881 cgcgacgatc aatccgcggc tgcagatccg gaccgtgtcg ctcgccgacg cgccgccggg
     5941 caacgcgaag gtgcgcgagc tcgcctcgct cgccggggac gcggactacc cggcctggtc
     6001 gccgga
//
LOCUS       DASTYP010000061         6195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_441470, whole genome shotgun sequence.
ACCESSION   DASTYP010000061 DASTYP010000000
VERSION     DASTYP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441470"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFV59_02980"
     CDS             <1..831
                     /locus_tag="VFV59_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5324824.1"
                     /translation="TLDATWRLAGELNAFDAGWLSDHLSDAGRPRGGPAFEAFTTAAA
                     LAHRVPGRWIGIAVASATFRHPAVVAKAATVLDNATGGRFILGMGAGWHAGEHDAYGI
                     DLPPLPERFDRFESAVRAVRALFSDAAATEEGVTLDDPIHPLRGATNLPSPVRAGGPQ
                     LWLGGQKRRGIAMAARWADGWPMPGNRPGDLDYFVSRRDALRAALLEAGRDPDAFSFA
                     AQVVCDVDAAGLRAARASAVAFRKAGATHVILGIRAADAPDGLRAIAREVADAIRDAG
                     "
     gene            complement(875..1378)
                     /locus_tag="VFV59_02985"
     CDS             complement(875..1378)
                     /locus_tag="VFV59_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324825.1"
                     /translation="MPVRSFVLRAGGVASMALLLVACSASSGSSSSASAAASAAPPSS
                     APASAAAGDATVVVADSDLGQILTDADGNVIYYFANDEEGVSNCEGDCLSNWPPVPVS
                     GDPSAGDGVSAELGTIDGNDGSTQLTVNGYPAYYFAGDQAAGDTNGQGVGGIWWVFGA
                     DGEPIES"
     gene            complement(1445..2326)
                     /gene="sucD"
                     /locus_tag="VFV59_02990"
     CDS             complement(1445..2326)
                     /gene="sucD"
                     /locus_tag="VFV59_02990"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU5324826.1"
                     /translation="MSILVNRDTRLLVQGITGREGEFHSTAMLEYGTTIVAGVTPGKG
                     GQSALDGRVPVYDTVAEAVEQTGANASCIFVPAAFAPDAMLEASAAGVPLVICITEGV
                     PALDMLRVYHVLRERGTRLIGPNCPGLTTVDEAKVGIIPGNIHARGPVGLVSRSGTLT
                     YEVVQAMTDAGMGQTTCVGIGGDPVIGTNFIEVLDLFNQDPETEAIVMIGEIGGEEEE
                     KAAAFCAREVRKPMAAFIAGRTAPPGRRMGHAGAIISSGGEGTAESKRRALEEAGIRV
                     ADSPSHIPQLLRDAGVR"
     gene            complement(2323..3465)
                     /gene="sucC"
                     /locus_tag="VFV59_02995"
     CDS             complement(2323..3465)
                     /gene="sucC"
                     /locus_tag="VFV59_02995"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU5324827.1"
                     /translation="MKLQEYRSKEILARHGVPLLAGEIATTPEQARAAAQDIGGQVVV
                     KAQVLVGGRGKAGGVKLAADPDEAAQRAEEILGLDIKGTIVKTVLVAPAADIAHEYYL
                     GLILDRAAKAITVIASAEGGVEIEETAKTNPGAILRLPLHPLIGLQAFEVSRVAFFLG
                     IPADLRKEFAKVMRGLYAAFVESDADLAEINPLVVTDDGRLLALDAKIVLDDSALPRH
                     ADLEAMRDLNEEEPSEIAAREAGISFIKLDGNIGCMVNGAGLAMTTMDLVKLAGGEPA
                     NFLDIGGGAKADKVAAAFRIILDDPNVQAILINIFGGITRGDEVARGIVEARQTLTRD
                     VPMVVRIVGTNAEEAKPILAQANLITADSLDDAAAKAVQAAKGAAA"
     gene            3633..4646
                     /locus_tag="VFV59_03000"
     CDS             3633..4646
                     /locus_tag="VFV59_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEU5324828.1"
                     /translation="MSYSVTLIPGDGIGPELAAATRTVLEATGIGFDWDVQEAGEATI
                     ATEGTPLPDRVIASIRANGVGLKGPITTPVGSGFRSVNVALRKELELYANVRPARSMK
                     GVESRYTDVDLIVVRENTEDLYAGVEHMVGADAAESIKIITRAASQRIARYAFEYAVA
                     NGRKKVTAVHKANIMKLSDGLFLESAQQVAAEYEGRVAFEDRIVDNMCMQLVQKPELY
                     DVLVLPNLYGDIVSDLAAGLVGGLGVAPGANIGEKAAVFEPVHGSAPKYAGQNKANPT
                     ALILSAALMLRHLGETAAADVVETAVRDVVAEGATVTYDLGGSAGTREFGDAVAARVR
                     AAA"
     gene            4661..5104
                     /gene="sdhC"
                     /locus_tag="VFV59_03005"
     CDS             4661..5104
                     /gene="sdhC"
                     /locus_tag="VFV59_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU5324829.1"
                     /translation="MGLAATPRRPAADGRPSRRRTPIVVKLFGYEMTWSELAWFGHRA
                     SGLGVLLYLFMHIIETSTVLFGPDVYNAAVGIFKNLPARAGEVVLMAAVVYHALNGLR
                     VIAMDFWPRLTVYYRPLTYGVIAATVISMIPLGLIMLRPFMPWGA"
     gene            5109..5558
                     /locus_tag="VFV59_03010"
     CDS             5109..5558
                     /locus_tag="VFV59_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEU5324830.1"
                     /translation="MTAQTRRARTIYANPSRAARPKPEASGRDRFWWYFMRLSGLALV
                     LLALGHMLIMHVLVELSGGEIDFAFVQSRWGTPFWRVYDFLLLLLAFIHGARGARTVI
                     TDYVTHRTARSLLIGILIALAVIWMVLGMAVIVFFDPNTAPPTGPFS"
     gene            5594..>6195
                     /locus_tag="VFV59_03015"
     CDS             5594..>6195
                     /locus_tag="VFV59_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005518565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5324831.1"
                     /translation="MRVHELDALVVGAGGAGLYAALELKQELGPEARIGVISKLYPSR
                     SHTGAAQGGVCAALANTEEDHWEWHWFDTVKGGDYLVDQDAAEVMCRDAIETIINLEH
                     LGLPFNRTPEGLIDQRRFGGHTRNYGEGPVRRSCFAADRTGHAILQTLFQQCIKHEVQ
                     FFDEYQVLDLAMPDGPAGAVTGVLALELATTELHAFRAKAV"
BASE COUNT          855 a         2222 c         2190 g          928 t
ORIGIN      
        1 acgctcgacg cgacgtggcg cctggccggg gagctgaacg ccttcgacgc cggctggctg
       61 agcgaccacc tctccgacgc cggccggccg cgcggtggtc cggccttcga ggccttcacg
      121 acggccgccg ccctggcgca ccgggtcccg ggacggtgga tcggcatcgc ggtggcctcg
      181 gcgacgttcc gccaccccgc ggtcgtggcc aaggcggcaa cggtcctgga caacgcgact
      241 ggcggccgat tcatcctggg gatgggcgcc ggctggcacg ccggagagca cgacgcctac
      301 gggatcgacc tgcccccgct gccggagcgc ttcgatcgtt tcgagtccgc ggtccgcgcg
      361 gtccgcgcgc tcttctctga cgcggcggcc accgaggagg gcgtgacgct ggacgacccc
      421 atccacccgc tgcgcggcgc caccaacctg ccgtccccgg tccgtgcggg cggcccgcag
      481 ctgtggctcg ggggtcagaa gcggcggggg atcgccatgg cggcgcgctg ggccgacgga
      541 tggccgatgc ccgggaatcg gcccggcgac ctcgactact tcgtgtcgcg ccgggacgcc
      601 cttcgcgcgg cgctgctcga agccggtcga gaccccgacg ccttctcgtt cgccgcccag
      661 gtcgtgtgcg atgtcgacgc ggccggcctg cgggcggcgc gcgcatcggc cgtcgccttc
      721 cggaaggccg gcgcgaccca cgtcatcctc ggcatccggg cggccgatgc gccggacggc
      781 ctgcgcgcca tcgcgcgcga ggtcgccgac gccatccggg atgccgggtg agcgctggcg
      841 tatatacgat atatacccca gcgccacacc gcccctagct ctcgatcggc tcgccgtcgg
      901 cgccgaacac ccaccagatc ccgccgactc cctggccgtt ggtgtcgccg gcggcctggt
      961 cgcccgcgaa gtagtacgcg ggatagccgt tcaccgtgag ctgcgtcgag ccgtcgttgc
     1021 cgtcgatcgt gccgagctcg gcggagacgc catcacccgc gctcggatca ccggagacgg
     1081 gcaccggggg ccagttcgag aggcagtcgc cctcgcagtt gctgacgccc tcctcgtcgt
     1141 tggcgaagta gtagatgacg ttgccgtcgg catccgtgag gatctggccg aggtcggagt
     1201 cggcgaccac gacggtggca tcgcccgccg cggcggacgc tggtgcgctc gacggcgggg
     1261 ccgcggaggc cgccgcggag gccgacgagg aggacccgct ggaggcgctg catgcgacga
     1321 gcagcagcgc catggaggcg acgcccccgg cgcggagaac gaacgaacga actggcatgg
     1381 tgaggctccc ggcatgtgga ctgtgccggg aactacggcc gaggttacgt gcggcgggcg
     1441 gagctcagcg gacgccggcg tcgcgcagca gctgcgggat gtgcgaaggg gagtcggcca
     1501 cgcggatgcc cgcctcctcc agcgcgcggc gcttcgactc ggccgtgccc tcgccaccgg
     1561 acgagatgat cgccccggcg tggcccatgc gccgccccgg cggggcggtg cgaccggcga
     1621 tgaaggccgc catcggcttg cgcacctcgc gcgcgcagaa cgccgccgcc ttctcctcct
     1681 cctcgccgcc gatctcgccg atcatgacga tcgcctcggt ctcaggatcc tggttgaaca
     1741 ggtccaggac ctcgatgaag ttcgtcccga tgaccgggtc gccgccgatg ccgacgcagg
     1801 tcgtctgacc catgccggca tcggtcatgg cctgcaccac ctcgtaggtc agggtgcccg
     1861 accgcgagac gaggcccacg ggaccgcggg cgtggatgtt gcccgggatg attccgacct
     1921 tggcctcgtc gacggtggtg aggcccgggc agttcggccc gatcagccgg gtgccgcgct
     1981 cgcggagcac gtggtagacg cgcagcatgt cgagcgcggg aacgccctcc gtgatgcaga
     2041 tgaccagcgg cacgccggcc gcggaggcct ccagcatggc gtcgggggcg aaggccgccg
     2101 gcacgaagat gcacgaggcg ttggcgccgg tctgctcgac cgcctcggcg acggtgtcgt
     2161 agacggggac gcggccgtcc agggccgact gaccgccctt gcccggcgtg acgccggcca
     2221 cgatggtcgt gccgtactcg agcatcgcgg tggagtggaa ctcgccctcc cgcccggtaa
     2281 tgccctgcac cagcaggcgc gtgtcgcggt tgaccaggat gctcacgccg ccgctccctt
     2341 cgcggcctgg accgccttgg ccgccgcgtc gtccagcgaa tcggccgtga tgaggttcgc
     2401 ctgcgccagg atcggcttgg cctcctcggc gttggtgccg acgatgcgca ccaccatcgg
     2461 cacgtcccgc gtcagcgtct ggcgtgcctc cacgatgccg cgggcgacct cgtcgccgcg
     2521 ggtgatgccg ccgaagatgt tgatcaggat ggcctgcacg ttcgggtcgt ccaggatgat
     2581 cctgaacgcc gccgcgacct tgtccgcctt ggcgccgccg ccgatgtcca ggaagttcgc
     2641 cggctcgcca ccggcgagct tgacgaggtc catggtcgtc atcgccaggc cggccccatt
     2701 gaccatgcag ccgatgttgc cgtcgagctt gatgaagctg atgccggcct cgcgcgccgc
     2761 gatctccgat ggctcctcct cgttgaggtc acgcatcgcc tccaggtccg cgtggcgcgg
     2821 gagggctgag tcgtccagca cgatcttcgc gtcgagggcc agcagccgtc cgtcatcggt
     2881 cacgaccagc gggttgatct cggcgaggtc cgcgtccgac tcgacgaagg ccgcgtacag
     2941 gccacgcatg accttcgcga actccttgcg gaggtcggcc gggatgccga ggaagaaggc
     3001 gacgcggctg acctcgaagg cctgcagccc gatgagcggg tggagcggca gccgcaggat
     3061 ggcgcccgga ttcgtcttcg ccgtctcctc gatctcgacg ccaccctcgg ccgaggcgat
     3121 gaccgtgatg gccttggccg cgcggtccag gatgagcccg aggtagtact cgtgcgcgat
     3181 gtccgcggcg ggagcgacga gcacggtctt gacgatcgtg cccttgatgt cgagcccgag
     3241 gatctcctcg gcccgctgcg ccgcctcgtc cggatccgcc gccagcttga cgccgccggc
     3301 cttgccgcga ccgccgacca ggacctgcgc cttgaccacc acctggccgc cgatgtcctg
     3361 ggcggccgcc cgcgcctgct ccggcgtggt cgcgatctcc ccggcgagga gcgggacccc
     3421 gtggcgcgcg aggatctcct tggaccgata ctcctggagc ttcactcaca tctcctgctt
     3481 gcgtggcgtc ggcgcacgcg ctcacggcgc tgggccaacg cggggatgtc accgattgta
     3541 gccgcggccc ccggcggtcg cccgggcggt ccgggctaga ctctggctcg gcacgccggc
     3601 gcgttgaccg gccgcacgag acgaggaatc cgttgagcta cagcgtcacc ctgatccccg
     3661 gcgacggcat cggtccggag ctggccgccg cgacccggac cgtcctggag gccaccggca
     3721 tcggcttcga ctgggacgtg caggaggccg gggaggccac catcgccacc gagggcacgc
     3781 cgctgcccga ccgggtcatc gcgtccatcc gcgccaacgg cgtgggcctc aagggaccga
     3841 tcacgacgcc tgttggctcc ggcttccggt cggtgaacgt ggcgttgcgc aaggagctgg
     3901 agctgtacgc caacgtccgt cccgcgcgga gcatgaaggg cgtcgagtcg cgctacaccg
     3961 acgtcgacct gatcgtggtg cgcgagaaca ccgaggacct gtacgcgggc gtcgagcaca
     4021 tggtcggcgc cgatgccgcg gagagcatca agatcatcac ccgcgccgcc agccagcgga
     4081 tcgcgcgcta cgcgttcgag tacgcggtgg cgaacggccg gaagaaggtc accgccgtcc
     4141 acaaggcgaa catcatgaag ctgtccgacg gcctcttcct ggagtcggcg cagcaggtcg
     4201 cggccgagta cgagggccgc gtggccttcg aggatcgcat cgtcgacaac atgtgcatgc
     4261 agctggtcca gaagcccgag ctgtacgacg tgctggtgct gccgaacctg tacggcgaca
     4321 tcgtcagcga cctggccgcg gggctggtcg gcggcctcgg cgtcgccccg ggtgccaaca
     4381 tcggcgagaa ggcggccgtc ttcgagccgg tccacggctc cgccccgaag tacgccggcc
     4441 agaacaaggc caacccgacc gcgctgatcc tctccgccgc cctcatgctg cggcacctgg
     4501 gcgagacggc cgccgccgat gtcgtcgaga cggcggtccg tgacgtggtc gcggagggcg
     4561 cgacggtcac ctacgacctg ggcggctcgg ccgggacccg ggagttcggc gacgcggtgg
     4621 cggcacgagt ccgtgccgcg gcctgacccg cgtcgctaga atcggactcg ccgctactcc
     4681 gcgccgtccc gctgcggacg gtcgcccgtc gaggagacgc acaccgatcg tggtcaagct
     4741 gttcggatac gagatgacct ggtccgagct ggcgtggttc ggacaccggg ccagcggcct
     4801 cggcgtgctc ctgtacctct tcatgcacat cattgagacg tcgaccgtgc tcttcgggcc
     4861 ggacgtctac aacgccgcgg tggggatctt caagaacctg ccggcgcgcg ccggcgaggt
     4921 ggtgctgatg gccgcggtcg tctaccacgc cctcaacggc ctgcgggtga tcgccatgga
     4981 cttctggccg cggctgacgg tctactaccg gccgctcacc tacggcgtca tcgcggcgac
     5041 cgtcatcagc atgatcccgc tggggctgat catgctgcgg cccttcatgc cgtggggcgc
     5101 ctgagccgat gaccgcgcag acgcgccgcg cccggacgat ctacgccaac ccctcgcgcg
     5161 ccgcgcgtcc gaagcccgag gcgagcggcc gggatcggtt ctggtggtac ttcatgcggc
     5221 tgtccggcct ggcgctcgtg ctcctcgcgc tgggccacat gctgatcatg cacgtgctgg
     5281 tggagctgtc cggcggcgag atcgacttcg ccttcgtgca gagccggtgg gggacgccgt
     5341 tctggcgcgt ctacgacttc ctgctcctgc tgctcgcctt catccacggc gcgcgcggcg
     5401 ctcggaccgt gatcaccgac tacgtgacgc atcggacggc gcgcagcctg ctgatcggga
     5461 tcctcatcgc gctggccgtg atctggatgg tgctgggcat ggcggtcatc gtcttcttcg
     5521 acccgaacac cgcgccgccg acggggccgt tctcgtgacc ctggccaccg tcggtcgccc
     5581 ggtcgtcccg cccatgcgcg tccacgagct ggacgcgctg gtcgtgggcg ccggcggagc
     5641 cggactgtat gccgcgctcg agctgaagca ggagctcggg ccggaggccc gcatcggcgt
     5701 catcagcaag ctgtacccct cgcggtcgca caccggcgcg gcccagggcg gcgtgtgcgc
     5761 cgcgctggcc aacaccgagg aggaccactg ggagtggcac tggttcgaca cggtgaaggg
     5821 cggggactac ctggtcgacc aggacgcggc cgaggtcatg tgccgcgacg cgatcgagac
     5881 catcatcaac ctcgagcacc tcggtctgcc gttcaaccgc acgcccgagg gcctgatcga
     5941 ccagcgccgc ttcggcggcc acacccgcaa ctacggtgag ggcccggtcc gtcgcagctg
     6001 cttcgccgcg gaccgcaccg ggcacgccat cctccagacc ctgttccagc agtgcatcaa
     6061 gcacgaggtg cagttcttcg acgagtacca ggtgctcgac ctcgccatgc ccgacggtcc
     6121 cgcgggcgcg gtcaccggcg tcctggcgct ggagctggcg accaccgagc tgcacgcgtt
     6181 ccgtgccaag gccgt
//
LOCUS       DASTYP010000062         5539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1133427, whole genome shotgun sequence.
ACCESSION   DASTYP010000062 DASTYP010000000
VERSION     DASTYP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133427"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFV59_03020"
     CDS             complement(<1..611)
                     /locus_tag="VFV59_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5324832.1"
                     /translation="MPALEQEVIARWRERNTMARYLARNEGSERRYSFLDGPITANNP
                     MGVHHAWGRTYKDLFQRFHTMLGERQRYQNGFDCQGLWIEVNVERELGFNNKRQIEEF
                     GIGRFVELCKARVDKYAAIQTEQSKRLGYWMDWDHSYYTNSDENNYTIWAFLAHCQRE
                     GLLYRGHDVMPWCPRCGTGISNMEAAEGYAEVKHTSVTVRLPIT"
     gene            complement(1010..2122)
                     /locus_tag="VFV59_03025"
     CDS             complement(1010..2122)
                     /locus_tag="VFV59_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324833.1"
                     /translation="MTDSASRIARVRLARSLPRLLALPVLLVVVAAAVAVAGLLLLPM
                     PAGAVAPVAAAVLVVVALWWAGRMLSVRLDVEESAVHIHWLGGERRYVLTPGPVTRVR
                     FRGENSSAVRARSSWLGGQLGAARLRDEEDIQIIRLAPTATAILVPTEDGRLAIAAAD
                     EEQLLQALTEAARARQRSEAVAPQPAEPEPEPEAPEPEPEPVTLDPRFMTGIERAAYE
                     RRLAEERDAAAVAALAAAEAEAARQEEQRLADEAGVPASTAAATATAEARPRARRWRP
                     RIGTRMERPRATMAFILLPLLGAGVAWGIGVASGRMPEPGTDLGRLTSLALVLAGPAT
                     SIAAIMARAWWPRIIGVVVAGGLAASVFIGRALLGG"
     gene            complement(2127..3782)
                     /locus_tag="VFV59_03030"
     CDS             complement(2127..3782)
                     /locus_tag="VFV59_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5324834.1"
                     /translation="MTNAEPRDRYHALGRLLEMVPGTISWAVLILPIWLSFSYPWLVA
                     YFVLSFDFYWLCRALWFSGAVIVAYRRIRRVLAVDWMDRLGHLERLLAEQRSVLSPAS
                     EQRSPAALGISLTGRVSSAERRRARQDLEDRRLVDALPADPPPLDEITHLALIPTYTE
                     SLEKLRHTVRALAEAEWPKERKICAIITRETDTGGRENVARLRDEFGDAFAEFLHILD
                     PLEPGIVVGKSSAMAWGGRYLYRLLVRERGMDPRRILVTDLDADYRVHPQYFAYLTWV
                     HLTDPNRETQLYQPIPYFHNNLWEAPFLQRLFAAVLTQLQMWRSVLPEKLQSFGSYST
                     TLHLVHDVGYWATDAIPEDSRFYWKSYFRYGDRFRAVPLFMPIYGDAVRARGYWRSMA
                     EQYLQARRWAWGVTDIPYVIENALNHPEIPFWSRFWRVLNLFGEHINWAIAPFVVMFG
                     ATVPLLINPAFGETTLGQNLPLFASVMLTIALVSLVVLIIVEHRIVPPRPADWGPVQR
                     FISYVQWLGLPFVGIFFSNLPALDAQTRLLTGRYLEYRVTEKA"
     gene            complement(3843..4769)
                     /locus_tag="VFV59_03035"
     CDS             complement(3843..4769)
                     /locus_tag="VFV59_03035"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU5324835.1"
                     /translation="METTTELWAFFRGEFVPLREANINVMTHGFNYGTGVFEGIRAYW
                     NADEEQLFALELLAHYERIRNSARLLMMDVKQSAQELADITVELLRRDGLREDVYIRP
                     IVYKSSETIGVRLHNLDSEITIFGVPFGQYIDTEGGVRAQVSSWRRTDDNAVPARGKI
                     TGAYVNSALAKSEAQLNGYDEAIVLTADGHVSEGSAENIFLIKDRTLVTPAATDNILE
                     GITRRRLIEIARTRLELPVVERSVDRTELYAADEIFLCGTGAQISPVIEVDRRAVGSG
                     RPGTITRELSRIYFDAVRGKVPEYREWLTPVY"
     gene            complement(4958..>5539)
                     /locus_tag="VFV59_03040"
     CDS             complement(4958..>5539)
                     /locus_tag="VFV59_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324836.1"
                     /translation="RLPGIRRRSALEVQFLPDVAEDEGDTTPRLGHEPRMRIVVGQVL
                     AAMVLIVIGAQVFVGSVDRIATAISFDHTILALIVAPIATELPEKFNSVLWVRNGKDT
                     LAMGNITGAMVFQSCIPTVLGLLFTAWAFTPENAISFASAGVAFAAVAIIFGIMLRTG
                     RLSARVLLLGGPLYLVYLWLALLSPLGGAAIGA"
BASE COUNT          839 a         1938 c         1941 g          821 t
ORIGIN      
        1 gtgatcggca gcctcacggt caccgaggtg tgcttcacct cggcgtagcc ctcggccgcc
       61 tccatgttgc tgatgccggt gccgcagcgc ggacaccacg gcatcacgtc gtggccgcgg
      121 tacagcagcc cctcgcgctg gcagtgcgcg aggaaggccc agatggtgta gttgttctcg
      181 tccgagttcg tgtagtacga gtggtcccag tccatccagt agccgaggcg cttgctctgc
      241 tcggtctgga tggcggcgta cttgtcgacg cgggccttgc acagctccac gaagcggccg
      301 atgccgaact cctcgatctg gcgcttgttg ttgaagccca gctcgcgctc gacgttgacc
      361 tcgatccaca gcccctggca gtcgaagccg ttctggtagc gctgccgctc gccgagcatg
      421 gtgtggaagc gctggaagag gtccttgtag gtccgccccc aggcgtggtg cacgcccatc
      481 ggattgttgg ccgtgatcgg gccgtccagg aagctgtagc gccgctccga cccctcgttg
      541 cgggccaggt agcgcgccat cgtgttgcgc tcgcgccagc gcgcgatgac ctcctgctcc
      601 agggcaggca tctccagctt gctcgatacc ggcttgaaca cagctcgcgt cttcgctcga
      661 ctctccgatg aacacagaac ccgccccgga actccgggac gggaacactc ccgcggtacc
      721 acccgcgctt gaccgatgcg cccgtcgggc gccgactcgg tccactcgtg gccgatgcta
      781 tcgggtgggc cgcacccggg agacgttacc cacgaggccg cgctgcatcc gcgcggtcgc
      841 ttggcgcctc cggctcggga gggatgaccc accgtcgccg tgccgcctgc tcacaccgtc
      901 caggctcgct cgtggtcgtg ctgacggccg ccgtggctcc gtcgtcgccg tcttcggttg
      961 ggcggcgatc gtcgcagcgc accgggacgc tgtcaattcg cgtcggggct cagcccccca
     1021 gcagcgcccg accgatgaag accgatgcgg ccaggccgcc ggcgaccacg acaccgatga
     1081 tgcgcggcca ccaggccctc gccatgatcg cggcgatgga ggtcgcgggg cccgcgagca
     1141 ccagcgcgag ggaggtgaga cgcccgaggt ccgtccccgg ctcgggcatg cggccggatg
     1201 ccacaccgat cccccacgcc acgccggctc cgaggagcgg cagcaggatg aacgccatgg
     1261 tcgcgcgcgg ccgctccatg cgggtgccga tccgcggccg ccaccgacgg gcgcggggtc
     1321 gcgcttcggc ggtggccgtc gccgcagccg tcgaggccgg cacgcccgcc tcgtcggcga
     1381 gccgctgctc ctcctgcctc gcagcctccg cctcggcggc agcgagggcg gcgaccgcgg
     1441 ccgcgtcgcg ttcctccgcc agccgccgct cgtaggcggc ccgctcgatg ccggtcatga
     1501 accgcggatc gagcgtgacc ggttccggct cgggctcggg cgcctccggc tcgggctcgg
     1561 gctcggccgg ctgcggtgca acagcctcac tgcgctgacg cgctcgcgcg gcctccgtga
     1621 gcgcctggag gagctgttcc tcgtcggccg cggcaatggc cagccggccg tcctcggtcg
     1681 gcaccaggat cgccgtcgcg gtcggcgcca gacggatgat ctggatgtcc tcctcgtcgc
     1741 gcagtcgggc ggcgccgagc tgcccgccga gccacgagct gcgggcacgc accgccgacg
     1801 agttctcgcc gcggaagcgc acccgcgtga cgggtccggg cgtcaggacg taccggcgct
     1861 cgccgcccag ccagtggatg tgcacggcgg actcctcgac gtcgagccga acgctcagca
     1921 tccgccccgc ccaccacagc gcgaccacca ccagcacggc agcggcgacg ggcgctacgg
     1981 caccggccgg catcggaagc agcaacagcc cggcgaccgc gaccgccgcg gcgaccacga
     2041 cgaggaggac gggcagcgcc aggagacgcg gcagtgagcg ggcgagccgc acgcgcgcga
     2101 tgcgcgaggc ggaatcggtc atggcatcag gccttctcgg tgacccggta ctcgaggtac
     2161 cgcccggtca gcagccgggt ctgggcgtcc agggctggga ggttgctgaa gaagatgccg
     2221 acgaacggca ggcccagcca ctgcacgtag ctgatgaacc gctggacggg gccccagtcg
     2281 gccgggcgtg gcggcacgat ccggtgctcg acgatgatca acaccaccag gctgacgagt
     2341 gcgatggtca gcatcacgct cgcgaacagc ggcaggttct gccccagggt cgtctcgccg
     2401 aacgccgggt tgatgagcag cggcacggtg gcgccgaaca tcaccacgaa cggtgcgatc
     2461 gcccagttga tgtgctcccc gaagaggttc agtacccgcc agaagcgcga ccagaacggg
     2521 atctcggggt ggttcagcgc gttctcgatg acatacggga tgtcagtcac accccaggcc
     2581 cagcgccgcg cctgcagata ctgctccgcc atgctgcgcc agtagccccg ggcgcgaacc
     2641 gcgtcgccgt agatcggcat gaacagcggg accgcgcgga accggtcgcc gtagcggaag
     2701 tagctcttcc agtagaagcg gctgtcctcc gggatcgcat cggtcgccca gtagccgacg
     2761 tcgtggacga ggtgcagcgt cgtcgagtag gacccgaagc tctggagctt ctcgggcagg
     2821 acgctgcgcc acatctggag ctgggtgagc accgcggcga agagccgctg gaggaacggc
     2881 gcctcccaca ggttgttgtg gaagtacggg atcggctggt agagctgcgt ctcccggttc
     2941 ggatcggtca ggtgcaccca ggtcaggtag gcgaagtact ggggatggac gcggtagtcc
     3001 gcatccaggt cggtgaccag gatgcggcgc gggtccatcc cccgctcccg caccagcagc
     3061 cgatacaggt agcggccgcc ccacgccatg gcgctggact tgccgaccac gatgcccggc
     3121 tccagcgggt cgaggatatg gaggaactcg gcgaatgcgt cgccgaactc gtcgcggagc
     3181 cgtgcgacgt tctcgcggcc gccggtatcg gtctcgcggg tgatgatcgc gcagatcttc
     3241 cgctctttcg gccactcggc ctctgcgagc gcgcggacgg tgtgccgcag cttctccagc
     3301 gactcggtgt aggtcgggat gagggcgagg tgggtgatct cgtcgagcgg cggcggatcc
     3361 gccggcagcg cgtcgaccag gcgccgatcc tccaggtcct gccgcgcccg tcgtcgctcg
     3421 gcggagctca cgcggccggt gagcgagatg cccagggccg ccggcgaccg ctgctcggac
     3481 gccggggaca gcaccgagcg ctgctccgca agcagccgct cgaggtggcc cagccgatcc
     3541 atccaatcga ccgccagcac ccgacggatg cggcggtagg cgacgatcac ggcgccgctg
     3601 aaccagagcg cccggcacag ccagtagaag tcgaagctca ggacgaagta cgccaccagc
     3661 cacgggtagc tgaagctcag ccagatgggc aggatgagga cggcccagga gatggtgccg
     3721 ggcaccatct cgagcagccg tccgagcgcg tggtatcgat cgcggggttc ggcgttcgtc
     3781 atcctcacct ggccggccgc gggccggtca gcgacgacgc cgatcccgcc gcgccgcttc
     3841 tcctagtaga ccggcgtcag ccactcgcgg tactcgggca ccttgccgcg caccgcgtca
     3901 aagtagatcc gcgacagctc gcgcgtgatc gtgccgggcc ggccgctccc gacggcccgc
     3961 cgatcgacct cgatcaccgg gctgatctgc gcgccggtgc cgcacaggaa gatctcgtcg
     4021 gccgcgtaca gctcggtgcg gtcgacgctg cgctcgacga ccggcagctc caggcgcgtc
     4081 cgggcgatct cgatcagcct gcggcgcgtg atgccttcca ggatgttgtc cgtcgccgcc
     4141 ggcgtgacca gggtgcggtc cttgatgagg aagatgttct ccgcgctgcc ctcgctgacg
     4201 tggccgtcgg cggtcagcac gatcgcctcg tcgtacccgt tgagctgggc ctcgctcttg
     4261 gccagcgcgg agttcacgta ggcaccggtg atcttgccgc gcgcaggcac ggcgttgtca
     4321 tccgtccggc gccagctgga cacctgggcg cggacaccgc cctccgtgtc gatgtactgg
     4381 ccgaacggga cgccgaagat ggtgatctcg gagtcgaggt tgtgcagccg cacgccgatc
     4441 gtctcggagc tcttgtagac gatggggcgg atgtagacgt cctcgcgaag cccgtcgcgc
     4501 cgcagcagct cgaccgtgat gtcggccagc tcctgggcgg actgcttgac gtccatcatg
     4561 agcaggcgcg ccgagttccg gatgcgctcg tagtgcgcga ggagctccag cgcgaagagc
     4621 tgctcctcgt cggcattcca gtaggcgcgg atcccctcga agacgcccgt cccgtagttg
     4681 aagccgtggg tcatgacgtt gatgttcgct tcgcgcagcg gtacgaactc gccgcggaag
     4741 aacgcccaca gctcggtggt ggtctccacg gacgccggct ggggtcggcg ttcctgcttc
     4801 agcactctgg cctccttgtc gtcgcgtcgg acggcgctgg tcgcacgcgc agcgacgcgc
     4861 gaccgagcgc aagaagtata ccgacgcacg ccggaccgcc cggtgggcca tccggcctct
     4921 cccggggtcc gatcgtccct ggcggcctcc acgcccgcta cgcgccgatg gccgcgccgc
     4981 cgagtgggct caacagggcg agccagaggt agaccaggta gaggggtccg ccgagcagca
     5041 ggacgcgggc ggagagtcgg ccggtgcgca gcatgatccc gaagatgatc gccacggccg
     5101 cgaacgccac gccggccgag gcgaagctga tggcgttctc cggcgtgaag gcccaggccg
     5161 tgaacagcag gccgaggacg gtggggatgc acgactggaa gaccatcgcg ccggtgatgt
     5221 tgcccatggc cagcgtgtcc ttaccgttgc gcacccacag cacgctgttg aacttctcgg
     5281 gcagctcagt ggcgatcggc gcgacgatca gcgccaggat ggtgtggtcg aagctgatcg
     5341 cggtcgcgat gcgatcgacc gaacccacga agacctgggc cccgatcacg atcagcacca
     5401 tggccgcgag cacctgtccg accacgatcc gcatgcgtgg ctcgtgaccg agccgtgggg
     5461 tcgtgtcgcc ctcgtcctcc gccacgtccg gcaggaactg gacctcgagc gccgaccgac
     5521 ggcggatgcc cggcagccg
//
LOCUS       DASTYP010000063        12919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1024082, whole genome shotgun sequence.
ACCESSION   DASTYP010000063 DASTYP010000000
VERSION     DASTYP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12919
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024082"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..518
                     /locus_tag="VFV59_03045"
     CDS             60..518
                     /locus_tag="VFV59_03045"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5324837.1"
                     /translation="MAHVRHELHIDAPIEHAFDLLMDAKRWPEWMSGGMEVKEVTGPL
                     DQVGTKVREESAFLGRRMESVTEIVEVQRPHLLKMVGESAGVKYHGMIRFTEAGAGTD
                     AEMESEYDLPAGFLGHIADRLFIERTMEREMRHNAENFKALAEAKVPALV"
     gene            616..1302
                     /locus_tag="VFV59_03050"
     CDS             616..1302
                     /locus_tag="VFV59_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEU5324838.1"
                     /translation="MCGRFSQSERVDRVAALFGATVDPDLPEGRYNVAPTDPIRILVE
                     TDGARRLVKADWGFRPFWRRDETRRTPGWINARAETALDSPAFGPALRRRRCLVPADA
                     FYEWDRSHRPPQPWAIGPTTADAVLLFAGIWTDALEDEPRTAAILTTGPNATMAPLHH
                     RMPVVLDPRDVDAWLDAEAPAEAAMGLLVPAPDDALRTWPVSPAVNKVGTDGAELLRP
                     LAPQPTLGLA"
     gene            complement(1306..2532)
                     /locus_tag="VFV59_03055"
     CDS             complement(1306..2532)
                     /locus_tag="VFV59_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU5324839.1"
                     /translation="MDSSERLRRYADLVVGVGANVQPDQSVMIQALVEHAELVRAIAE
                     AAYVAGARRVVVDYTDDHVQRSRLLHAPADSLASFDPWEKDRYAQLRADGGAFIRLSG
                     DPDVHLFDGIDPARLAAIPVELMRFFTDEVLFGGDVAWTIVAAPNRGWAQQVFGEPDL
                     ERLWDAVALAMRLDAADPVAAWRERAAELRDRQARLDRLGADAIRFRGPDTDLTVGLI
                     PGSRWHSATMQTARGTTYMPNLPTEEVFTSPHRDRAEGHVRITKPFVLPSGASVSGLQ
                     LRFEGGRIVGVTADDGAAAVEAVLERDENARRLGEVSIVTGDSAVARAGLLFHNTLFD
                     ENVGCHIAFGISFPFTLPDGMRMTEDERVQAGLNHASVHVDVVIGSPEVNVDAQLPDG
                     RLVPVLRDDRWVLEEP"
     gene            complement(2680..3030)
                     /locus_tag="VFV59_03060"
     CDS             complement(2680..3030)
                     /locus_tag="VFV59_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324840.1"
                     /translation="MDDATLRGAVEELPAYHVCGIQRDRARQVDGHDHVLFVETRDPD
                     GGQTRWRCVDVIAAMRDGERFVLGEGDGRRQLEPGLCAACAFVTVRVIPSDDAGADAY
                     AFGDVHSFWSGDPI"
     gene            complement(3299..4609)
                     /locus_tag="VFV59_03065"
     CDS             complement(3299..4609)
                     /locus_tag="VFV59_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5324841.1"
                     /translation="MNPSTQRTRKVIVVDNSHVARRIGERIRQVRTRAGLTQAQLAKG
                     RYTAAYISALERGLAKPSMAALTFISDRLGVPVPDLVSEERPAAGRLEADLMLAGGQH
                     QAALDRYEALLDEVGDDRRGRAELLRGRSEALCRLDRGVEAIASASESAELFDVLGAP
                     ADAAWSRYWLAFAHYQADNPSEARGILLQLLATERAGASVAPDFRFRLLTALGAVETW
                     DGESQRGLTYMEEARALLAEASLRQRAAFLSSLALQYRRVGDLERSLRTGYESLALYR
                     AADAEREEAALENNLAITFLEVGNTARADEHLGRARELAERHADARLLSEVAEAGARV
                     AHARGDEAAAAQHAAAAIDAVASGGSYLAAVGGHLILGRIAAARGVPEEAEAQFGRAA
                     DLLRGHRAHSQLRDVLGEWAELRSGWGDLAGANALYAEALRRPR"
     gene            4882..6255
                     /locus_tag="VFV59_03070"
     CDS             4882..6255
                     /locus_tag="VFV59_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5324842.1"
                     /translation="MTDPQLQDRVIALAEELQVPGVAVGVFHDDEERYAFHGVTSVEN
                     PLPVDDRTLFQFGSTGKTFTAVAILRLVDEGRVDLDAPVRRYVPELRLRDPDVAESIT
                     VLQLLNHTAGWDGDDFEDTGDGDDAIARFVDHMAELQQISPPGFIVSYNNAALALAGR
                     VIEKVTGSVYERAIRDLVLDPIGLTNTFFFANEVMTRRFVVGHRREPDGTQRVLRPWA
                     MPRSGNPMGGMAASAPDQLRWARFHLDHGRTADGRQVLDAALIDRMQQSTVEMPGSDL
                     GDAVGISWLLSTIGGERVVAHGGSTLGQYAGFVMVPSRRFAIVSLTNGEPNGPLFNRR
                     MRDWALATYLDLHRTTPVAEARSADALAEYAGTYETVSSVIAIAADGGRLRMEVDAKP
                     AFLEQLGSDADDENEPPIPLGMIAGGDRYVVPEGSHEAETGFFVRDDRGRIMGLHVHG
                     RYAPRCP"
     gene            complement(6320..6844)
                     /locus_tag="VFV59_03075"
     CDS             complement(6320..6844)
                     /locus_tag="VFV59_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324843.1"
                     /translation="MDLLVLRLVHIGAGVFWAGSVYAFFLFVQPAAGDAAPSSTPFVY
                     ELLHGRRFSQIILAAAATTVIAGLWLLWRTSNGFDPGVLFGIGRLGYTVGGTAGILTF
                     ALGGGYVFPRTMTVERTIGTILAESRPPTSDEQTLLARTAKESRRAGWFVLAGLAIAV
                     VSMATAQYWSLLVS"
     gene            complement(6932..7222)
                     /locus_tag="VFV59_03080"
     CDS             complement(6932..7222)
                     /locus_tag="VFV59_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324844.1"
                     /translation="MNKRRALLALRSIDVSYRSAIRHAPASAPRQQEWLGYARQRAEQ
                     LLDTLEAQLLKEGVDSEIALQLRVVRDSIRRADNGAWGSDPHDAGRSKESAG"
     gene            7345..7614
                     /locus_tag="VFV59_03085"
     CDS             7345..7614
                     /locus_tag="VFV59_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324845.1"
                     /translation="MNQPRLVSVNTRLSAPDLGLDVEIRLRQLDGRWLAVADFGGEPE
                     VGIGATPRTALAAALATLPRRAATVLMADPQLLGLSLAIRHPVPA"
     gene            7663..8181
                     /locus_tag="VFV59_03090"
     CDS             7663..8181
                     /locus_tag="VFV59_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU5324846.1"
                     /translation="MDEQTRERDRRGMERAIEQARAGRAEGGVPIGATLMADDGTVLG
                     EGRNRRVQDGSAIRHGETDALENAGRLPASVYRRSTMFTTLSPCDMCTGAILLYGIPR
                     VVIGENTTFLGGEDLLRSRGVEVVNVHSAECVELMREFIAARPDVWYEDIGEEAAAGL
                     VAGKDSDRPIES"
     gene            complement(8178..8693)
                     /locus_tag="VFV59_03095"
     CDS             complement(8178..8693)
                     /locus_tag="VFV59_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324847.1"
                     /translation="MRRLFFLAALAAALYAVATWWRDHRRVGTGFVNRVVNPWLERRG
                     VIGATRGELAVIEHVGRKSGIVRRTPIHPMPVTDGYRIIVPVGERSEWARNVLAAGHC
                     RLILADRVVELDEPVLRAPFDVPDLARPMRALFGWLGFRYLELHTLGEVSAASVERDV
                     AEPATPEVVPV"
     gene            complement(8768..9751)
                     /locus_tag="VFV59_03100"
     CDS             complement(8768..9751)
                     /locus_tag="VFV59_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5324848.1"
                     /translation="MRRFIGFALVALAVMAGAVVALAWLVSTLIGQQVITAVLPALAF
                     VIALLIAARLVRGLWREAAPLGDLIEAASRVEAGQVGTQVRVSGPPEVRSLARAFNAM
                     SARLAQDTEGQRRLLADVSHELRTPLSVIRGNLEGMIDGLYPTNREHLEVVLAETRQM
                     EQLVEDLRTLSLADAGALQLHREPIDLRALAGEVVAAFQPEADEAGVTLALAADAVPS
                     LAADPRRLRQVLANLLSNAVRHTPSGGRVDVRVEHENGAVVLEVADTGVGMDAAALDR
                     AFDRFWRAGDRSGAGLGLAIARDLVEAHGGAVSLESQLGEGTRVRCRLPLG"
     gene            complement(9903..10580)
                     /locus_tag="VFV59_03105"
     CDS             complement(9903..10580)
                     /locus_tag="VFV59_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5324849.1"
                     /translation="MARILIVDDEPKIVRLVVDYLAAAGFTVSTAGTGNEALMRVRTE
                     RPDLVVLDLGLPGLDGLDVTRAIRRSGELPIIMLTARDDETDRVLGLELGADDYVTKP
                     FSPRELVARVRAVLRRHGGAPTPNEQLRAGDLVLDVGRMRVTRAGEPVELTATEFALL
                     AAMARQPGRVFTRSQLLDAVHGEAFEAYERAIDAHVKNIRRKVEPDPRQPRYLLTVYG
                     VGYRLAD"
     gene            complement(10604..10993)
                     /locus_tag="VFV59_03110"
     CDS             complement(10604..10993)
                     /locus_tag="VFV59_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324850.1"
                     /translation="MSGFWRIAAAVLAIAIAVGIGVAVYNAGVSAGFTEAARQAAESG
                     EVVAPYAPGWVGPYWHGGWGYGFGFFGIFFWILGFFLIIGLLRAAFGWGRWGGPGRGP
                     GGGAWGGRSERAEEWHRELHRRESASG"
     gene            complement(10990..11187)
                     /locus_tag="VFV59_03115"
     CDS             complement(10990..11187)
                     /locus_tag="VFV59_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324851.1"
                     /translation="MRTDERQRASTPPVQRILAVLAGVMLLRILLRHGPRGPMRDRRR
                     AMIAKLHRELHAADAADEVTA"
     gene            complement(11311..11559)
                     /locus_tag="VFV59_03120"
     CDS             complement(11311..11559)
                     /locus_tag="VFV59_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324852.1"
                     /translation="MEASHLLLAQTLSLCGLIGGAAAYAGLIRRRAERLIDERAALAS
                     QAAVERVGRTLALGHIEHTFLRGHYPESYRGSWARRRR"
     gene            complement(11638..12126)
                     /locus_tag="VFV59_03125"
     CDS             complement(11638..12126)
                     /locus_tag="VFV59_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324853.1"
                     /translation="MTADAEPTAVGADVHRRLAADLFNHTWTLLETEDRTADQDAEMI
                     HAAHASVFHWMRVDPPDVRQRRAVGEWQCARVYSVLGRAEAALHHARACLALAEGGPV
                     DDWVLAAAYEAMARASRVAADRDAFVQWREMAEAATAAIADPADRQVIEGDLATLGDL
                     RS"
     gene            12253..12486
                     /locus_tag="VFV59_03130"
     CDS             12253..12486
                     /locus_tag="VFV59_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324854.1"
                     /translation="MNWFDEVTGRARRRRLEEELTRADAEIWRLRQLLAEATRSPMEF
                     ARMREREVALRRLTRLRIAGEGHRDTERTLRAG"
     gene            complement(12551..>12919)
                     /locus_tag="VFV59_03135"
     CDS             complement(12551..>12919)
                     /locus_tag="VFV59_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324855.1"
                     /translation="ASADPAIGRALTGMSLAELQQDGPAAYRLFDFQSRVNGWSLVLI
                     GVLLAAVIVGPYRRRESWAWLAAWTLPAWAAGVPLFYLVAGLAPDEAPPPPAISGPIV
                     AIVAVVLLLLDRPVTSQPRG"
BASE COUNT         1959 a         4614 c         4586 g         1760 t
ORIGIN      
        1 tgacgctcca ttccggcgcc gattcgctcg tcaccggcct cggcagagga ggatgaccca
       61 tggcacacgt gaggcatgag ctgcacatcg acgccccgat cgagcacgcc ttcgacctgc
      121 tcatggatgc gaagcgctgg ccggagtgga tgtcgggtgg catggaggtg aaggaggtca
      181 ccggacccct ggatcaggtc gggacgaagg tccgcgagga gtcggcgttc ctcggccgga
      241 ggatggaaag cgtcacggag attgtcgagg tgcagcgacc gcacctcctg aagatggtcg
      301 gcgagagtgc cggcgtgaag taccacggca tgatccggtt cacggaggcc ggggcgggaa
      361 ccgacgccga gatggagagc gagtacgacc tcccagccgg cttcctcggc cacatcgccg
      421 atcggctgtt catcgagcgg acgatggagc gcgagatgcg ccacaacgcc gagaacttca
      481 aggcgcttgc cgaggcgaag gtgccggcgc tcgtctgatc tccggtcatt cgtcccatcc
      541 gaggacggca cggcgacccc gacgctcgcc gccggggtcg ccctcgcgtc cgtcagcagc
      601 gcctaccatc cgagcatgtg cggccgcttc tcccagtccg aacgcgtgga ccgcgtggcg
      661 gcgctgttcg gcgcgaccgt tgaccctgac cttcccgaag gccgctacaa cgtcgcaccc
      721 accgatccca tccggatcct ggtcgagacc gatggcgcac gccgcctggt caaggccgac
      781 tggggcttcc ggccgttctg gcgccgcgac gagactcgcc gcacaccggg ctggatcaac
      841 gcccgagccg agaccgccct cgactccccc gccttcggcc cggcactccg ccggcgccga
      901 tgcctggtgc ccgccgacgc cttctacgag tgggatcggt cgcatcggcc accgcagccc
      961 tgggcgatcg gacccacgac cgctgacgcg gtcctcctct tcgccggcat ctggaccgat
     1021 gccctcgaag acgagccgcg cacggccgcc atcctgacca ccggcccgaa cgcgaccatg
     1081 gcgcccctcc accaccgcat gccggtggtg ctcgacccgc gggacgtcga cgcctggctg
     1141 gacgcggagg cgccagccga ggcggcgatg gggctcctgg tccccgcccc ggacgatgcg
     1201 ctccgaacgt ggccggtcag tccggccgtc aacaaggtcg ggactgacgg tgcggagctg
     1261 ctgcggccgc tggcgccgca accgaccctc ggcctcgcct gagggtcagg gctcctcgag
     1321 cacccaccga tcgtcgcgca gcaccggcac gagccgccca tcggggagct gggcgtcgac
     1381 gttcacctcc gggctgccga tgacgacgtc gacgtggacc gatgcatggt tcagcccggc
     1441 ctggacgcgc tcgtcctcgg tcatccgcat cccgtccggc agggtgaagg ggaagctgat
     1501 cccgaatgcg atgtggcacc cgacgttctc gtcgaacagg gtgttgtgga agagcagacc
     1561 ggcccgcgcc accgctgagt cgccggtgac gatcgagacc tcgcccagac gtcgcgcgtt
     1621 ctcgtcgcgc tcgagcaccg cctccacggc ggccgctccg tcgtcggccg tgaccccgac
     1681 gatccgcccc ccttcgaacc ggagctggag cccggagacg gaggcgccgc tcgggaggac
     1741 gaacggcttg gtgatgcgga cgtgcccctc ggcccggtcg cggtgcgggc tcgtgaagac
     1801 ctcctcggtg ggcaggttcg gcatgtaggt ggtgccgcgc gccgtctgca tcgtggcgct
     1861 gtgccatcgc gagccgggga tcagtccgac cgtgaggtcg gtgtccggtc cgcggaagcg
     1921 gatggcatcg gcacccaggc ggtccagtcg agcctgccgg tcgcgcagct cggcggcgcg
     1981 ttcgcgccag gcggcgacgg ggtcggccgc gtcgaggcgc atggccaggg cgactgcgtc
     2041 ccagagtcgc tcgaggtccg gctcaccgaa gacctgctgt gcccagccac ggttcggagc
     2101 ggcgacaatg gtccatgcca cgtcgccgcc gaacaggacc tcgtcggtga agaagcgcat
     2161 cagctcgacc gggatggccg cgaggcgcgc cggatcgatg ccgtcgaaca ggtgcacgtc
     2221 ggggtcgccc gagagccgga tgaaggcgcc gccgtcggcg cggagctgcg cgtatcggtc
     2281 cttctcccag ggatcgaagc tggccaggga atcagccggg gcgtggagca gacgcgaccg
     2341 ctgcacgtgg tcgtccgtgt aatcgacgac caccctccgg gccccggcga cgtacgcggc
     2401 ctcggcaatc gctcgcacca gctccgcgtg ctcgaccagc gcctggatca tgacgctctg
     2461 gtcgggctgg acgttggcgc cgacgccgac caccaggtcg gcgtaccgcc gtagccgctc
     2521 ggaggaatcc atggttcacc tcagccgtgc accgctcgcc ggactgtagt cggtggccac
     2581 ggcgcggtga aggccgcggc ggcgcgcccg tccctcaggg cccgagcgaa gggcggcccg
     2641 agcgagaacg atcagcgccc gacagtgcgg acgcaacggt cagatggggt cgccgctcca
     2701 gaaggagtgg acgtcgccga aggcataggc gtcggcaccg gcgtcgtcgg acggaatcac
     2761 tcgcacggtg acgaacgcgc aggcggcgca caggccgggc tcgagctgcc ggcggccgtc
     2821 gccctccccg aggacgaacc gctcaccgtc gcgcatggct gcgatcacgt cgacgcaccg
     2881 ccaccgcgtc tggcctccgt cggggtcacg cgtctcgacg aacagcacgt gatcgtgtcc
     2941 gtcaacctgc ctggcgcgat cccgttggat cccgcagacg tggtaggccg gaagctcctc
     3001 gaccgccccc ctcagcgtgg cgtcgtccat cggctcattg ataatagttg atcccccggc
     3061 ggaacacgcg tacgatcggg ccatccgggt ggggcgggcc ggtgagcgga aagggacact
     3121 gtccatgcgt cgtatccgct cgtacctggt ctccctgtcg ttctccgcga tcctcacgtt
     3181 gctctccgcg gccgcagccc tggccgacag ccagccgccg ttcccgcgat agcccgctcc
     3241 cgaaccgcgt cgctgatctc cagccggtaa gtgctcaccg gccgtgccca cccgaggatc
     3301 agcgagggcg cctgagcgcc tccgcgtaga gggcgttggc gcccgccagg tcgccccatc
     3361 ccgaccgcag ctccgcccac tcgccgagca cgtcgcgcag ctgggagtgc gcgcgatgcc
     3421 cccgcagcag gtccgccgcc cgcccgaact gggcctcggc ctcctcgggt acgccgcgag
     3481 ccgcggcgat cctgcccagg atgaggtggc cccccaccgc cgcgaggtag ctcccgcccg
     3541 acgccacggc gtcgatggcg gcggcggcgt gctgggcggc cgccgcctcg tcgccacgtg
     3601 cgtgagcgac gcgggcccct gcctcggcca cctccgagag gaggcgtgcg tccgcgtgcc
     3661 gctccgccag ctcgcgtgcg cgccccaggt gctcgtccgc acgagcggtg ttcccgacct
     3721 ccaggaacgt gatcgccagg ttgttctcga gcgccgcctc ctcacgctcg gcgtcggccg
     3781 cacggtacag cgccaggctc tcgtaacccg tgcgcagcga gcgctcgagg tcgccgacgc
     3841 ggcggtactg cagcgccagc gacgagagga aggcggcgcg ctgccgcagc gaggcctccg
     3901 ccagcagcgc gcgggcctcc tccatgtacg tcagtccgcg ctgcgattcg ccgtcccagg
     3961 tctccaccgc gcccaacgcg gtgagcagcc ggaaccggaa gtccggcgcg accgacgcgc
     4021 cggcccgctc cgtggcgagg agctgcagca ggatgccgcg agcctcggac gggttgtccg
     4081 cctggtagtg cgcgaacgcc agccagtacc gcgaccaggc ggcatcggcg ggtgcgccga
     4141 gcacgtcgaa cagctcggcc gactcggagg ccgatgcgat cgcctcgacc ccgcggtcca
     4201 gacggcacag cgcctcggac cggccccgca gcagctcagc gcgaccgcgt cgatcgtccc
     4261 ccacctcgtc caggagcgcc tcgtagcggt cgagggcggc ctggtgctgc cccccggcga
     4321 gcatcaggtc cgcctccagc cggccggccg cggggcgctc ctccgagacg aggtcgggca
     4381 ccggcacccc aaggcgatcg ctgatgaagg tcagcgccgc catggacggc ttggccaggc
     4441 cgcgttccaa cgcgctgatg taagccgcgg tgtatcggcc cttcgccagc tgcgcctgcg
     4501 tgagccccgc acgcgtccgg acctgccgga tgcgctcgcc gatgcggcgc gccacgtggc
     4561 tgttgtcgac cacgatgacc ttgcgggtcc gctgcgtgct cggattcacc accgcactat
     4621 acggcgcgga ttcaagcgat tgttgagtgg cgcctcagat cacgacggcg ccttcgccat
     4681 tcacgcccgg agtggtcgaa cgccggttcg cgccggccga ggctgccact tccccgggtg
     4741 cgcacaactc gctggcgtga atggcgaagg ggagtcggcc ccggaggcgc cgttcacgtc
     4801 caccagtggt atgtggccga ggcggagcgc gcgaacgcgc cggcggttgg taacctagcc
     4861 gccgagcatt cgaggggagt cgtgaccgat ccgcagctcc aggaccgcgt catcgcgttg
     4921 gcggaggagc tccaggtccc gggtgtcgcg gtcggcgtgt tccacgacga cgaggagcgc
     4981 tacgccttcc atggcgtgac gagcgtcgag aacccgcttc ccgtcgatga ccggaccctc
     5041 ttccagttcg gctcgacggg caagaccttc acggccgtgg ccatcctgag gctggtggac
     5101 gagggccgcg tggacctcga cgcgccggtg aggcgctacg tgccggagct ccggttgcgt
     5161 gacccggacg tcgccgagtc catcaccgtc ctccagctcc tcaaccacac cgccggctgg
     5221 gatggcgacg acttcgagga caccggcgac ggcgatgacg ccatcgcgcg cttcgtcgat
     5281 cacatggcgg agctgcagca gatcagcccg ccggggttca tcgtgtcgta caacaacgcg
     5341 gcgctcgccc tggcggggcg cgtcatcgag aaggtgaccg gatcggtcta cgagcgcgcc
     5401 attcgtgacc tggtgctgga tcccatcggc ctgaccaaca cgttcttctt cgcgaacgag
     5461 gtcatgacgc ggcggttcgt cgtcgggcac cgccgcgagc ccgacggcac gcagcgcgtc
     5521 ctgcgaccgt gggccatgcc ccgcagcggc aacccgatgg gcggcatggc cgcatcggcc
     5581 cccgaccagc tgcgctgggc gcggtttcac ctcgaccacg ggaggaccgc cgacgggcgc
     5641 caggtgctgg atgcggcgct gatcgatcgg atgcagcagt cgacggtcga gatgcccggc
     5701 tcggacctcg gcgatgcggt cggcatctcg tggctgctca gcaccatcgg tggtgagcgc
     5761 gtggttgccc acggcggatc gacgctgggt cagtacgcgg gcttcgtgat ggtgccgtcg
     5821 cgtcggttcg cgatcgtgtc gctgaccaac ggcgagccca acgggccgct cttcaaccgc
     5881 cgcatgcgcg actgggcgct ggccacgtac ctggacctgc accggacgac gccggttgcc
     5941 gaagcacgct cggccgatgc gctggccgag tacgccggca cgtacgagac cgtgtcgagc
     6001 gtcatcgcga tcgccgccga cggcggtcgg ctacgcatgg aggtcgatgc caagccggcc
     6061 ttcctggagc agctcggatc cgacgccgac gacgagaacg agccgccgat tccgctcggc
     6121 atgatcgccg gcggcgatcg gtacgtcgtt cccgaggggt cgcacgaggc tgagaccggc
     6181 ttcttcgtgc gcgacgaccg cggccggatc atgggcctgc acgtccacgg acggtacgcg
     6241 cctcgctgtc cgtgatcatc gcgccagcgc aggccgtcat gcgccgtacg ccgcgtcggg
     6301 atcgcggggt ctcagaccct caggaaacga gcaggctcca gtactgcgcg gtcgccatgc
     6361 tgacgacggc gatcgcgagg ccggccagca cgaaccagcc ggcccggcgc gactccttcg
     6421 cggtccgcgc caggagcgtc tgctcgtcgc tcgtcggcgg ccggctctcg gcgaggatgg
     6481 tgccgatggt gcgctcgacc gtcatcgtgc gcgggaagac gtagccgcca cccagggcga
     6541 acgtcaggat ccccgccgtc ccacccaccg tgtagccgag ccgcccgatg ccgaacagca
     6601 cgccggggtc gaagccgttg ctggtccgcc acagcagcca gaggcccgcg atgacggtcg
     6661 tggcggcagc agccaggatg atctgcgaga agcgtcgacc gtggagcagc tcgtacacga
     6721 acggcgtgga cgagggtgcg gcgtcgccgg cggcgggctg gacgaacagg aagaacgcgt
     6781 agaccgatcc cgcccagaac acccccgcac cgatgtgaac gagccgcagc accagaagat
     6841 ccattcgtgg agccctcgag acgaccgatg ggcgggcagc cgcggcagga tagtcccctc
     6901 gacccgtcat gggaatacgc gcccgggatc gctagccggc ggactccttc gatcggcccg
     6961 cgtcatgcgg atcgcttccc catgctccgt tgtccgcgcg tcggatgctg tcccgcacga
     7021 cccgcaactg gagcgcgatc tcggagtcga cgccttcctt cagcagctgc gcctcgagcg
     7081 tgtcgagcag ctgctcggca cgctggcggg catacccgag ccactcctgc tggcgaggtg
     7141 cggaagcagg ggcatggcgg atcgcggacc gatacgagac gtcgatcgat cggagggcga
     7201 ggagggcgcg gcgcttgttc attcggacag ggtcggtacg tgcgaggtgg gggccgctcc
     7261 agcaagaccc ctgccggggc atgccacctc ctgccgccgg atcgcgacgc tcgagctgtc
     7321 acgatgcgtc ctcacgctcg gaccatgaat cagccacggc tcgtcagcgt gaacacgcgg
     7381 ctcagcgcgc cggacctcgg actcgacgtg gagatccgcc tgcgccagct ggatggacgc
     7441 tggctcgcgg tcgcagactt cggcggagag ccggaggtcg ggatcggcgc cacaccgcgg
     7501 acggcgctcg ccgcggcgct ggccacgctt ccccggcggg ccgccacagt cctgatggcg
     7561 gatccgcagc tgctcgggct gagcctggcc attcgtcatc cggttcccgc ctagggagga
     7621 cgcgcacaat gcgtcccacg ctgcaggtga ggaggcgcac gggtggatga acagacgcgg
     7681 gagcgtgacc ggcgcgggat ggagcgagcc atcgagcagg cgcgggccgg acgcgccgag
     7741 ggcggcgtgc ccatcggcgc cacgctcatg gcggacgacg gcacggtcct cggcgaaggg
     7801 cgcaaccgtc gcgtccagga cggcagcgcc atccgccacg gcgagaccga tgcgctggag
     7861 aacgccggcc ggctgccggc atcggtctac cggcgctcga cgatgttcac cacgttgtcg
     7921 ccgtgcgaca tgtgcaccgg tgcgatcctg ctgtacggga tcccgcgtgt ggtcatcggc
     7981 gagaacacga cgttcctcgg cggagaggac ctgttgcggt ctcgcggcgt cgaggtggtg
     8041 aacgtgcact ccgccgagtg cgtggagctg atgcgcgagt tcatcgccgc gcgaccggac
     8101 gtgtggtacg aggacatcgg ggaggaggcg gcggccgggc tggtggcggg gaaggactcg
     8161 gaccggccga tcgagagcta gacggggacg acctcgggcg tcgcaggttc ggcgacgtct
     8221 cgctcgaccg aggcggcgga cacctcgccg agcgtgtgca gctcgaggta gcggaacccc
     8281 agccatccga acagcgcccg catgggccgc gccaggtcgg ggacgtcgaa cggggcgcgg
     8341 aggaccggct cgtcaagctc cacgacccgg tcggccagga tgagccggca atggccggcc
     8401 gccaggacgt tgcgcgccca ttccgagcgc tcgcccacgg ggacgatgat ccggtagccg
     8461 tcggtcacgg gcatcgggtg gatgggcgtg cgccggacga tcccggactt ccggccgacg
     8521 tgctcgatca ccgccagctc cccacgcgtg gcgccgatga cgccgcgacg ctccagccac
     8581 gggttgacga cgcggttgac gaagccagtg cccacgcgac ggtggtcgcg ccaccaggtc
     8641 gccacggcgt agagcgcggc agcgagtgcg gcgaggaaga agagcctgcg catgcgagtc
     8701 ccctccgttt tcgcggaggc tcccgtagcc gtcaggctgc ttccagtgcc gaacggacgt
     8761 aaggcggtca gcccagcggc aggcggcaac ggactcgagt tccctcgccc agctggctct
     8821 cgagggacac cgcgcccccg tgggcctcca ccaggtcacg cgcgatcgcc agtccgaggc
     8881 ccgcaccgga acgatccccc gcccgccaga agcggtcgaa cgcgcggtcg agggcggcgg
     8941 cgtccatgcc cacgccggta tcggccacct cgaggacgac ggcaccgttc tcgtgctcca
     9001 ccctcacgtc gacgcggccg cccgagggcg tgtggcgaac ggcgttcgac aacaggttgg
     9061 cgagcacctg gcgaaggcgg cgcggatcgg cggccaggct gggaaccgca tcggcggcca
     9121 gcgccaacgt cacgccggct tcatcggcct cgggctggaa ggcggccacg acctctccgg
     9181 ccagggcgcg caggtcgatc ggctctcggt gcagctggag cgcgcccgca tcggccaggg
     9241 agagcgttcg gagatcctcc accagctgct ccatctgtcg cgtctcggcc aggaccacct
     9301 cgaggtgctc ccgattggtg gggtacaggc cgtcgatcat cccctccagg ttgccgcgaa
     9361 tgacgctcag cggggtgcgg agctcgtggc tgacgtccgc cagcagtcga cgctgaccct
     9421 cggtgtcctg ggccagcctc gcgctcatcg cattgaatgc tctggccaac gagcgcacct
     9481 cgggcggccc gctgacccga acctgggtgc cgacctgccc ggcctcgaca cgggaggctg
     9541 cctcgatcag gtcgccgagc ggggcagcct cgcgccacag gccgcgcacc agccgggcgg
     9601 caatcaacag cgcgatcacg aacgccagcg cgggcagcac ggcggtgatg acctgctgcc
     9661 cgatcagcgt gctcaccagc cacgcgaggg ccacgacagc gccggccatc acggcgagcg
     9721 cgacgagcgc gaagccgatg aagcggcgca tgaagcggcg tcgaacgccg cgccacgcgg
     9781 cgggatcctg tggcggccag ggctcgccct cgggccacca cggcggccgg cgactgccgc
     9841 tccaccctcg gctcgagccg tgccggcccc agcgttggcc gcctgacccg cgatgcggag
     9901 cgtcagtcgg caaggcgata gcccacgccg tagaccgtga ggaggtagcg cggctggcgc
     9961 ggatccggct ccaccttgcg gcggatgttc ttcacgtgtg cgtcgatcgc ccgctcgtag
    10021 gcctcgaacg cctccccatg cacggcgtcg agcagctgcg agcgcgtgaa cacgcggcca
    10081 ggctgtcgcg ccatcgcagc aaggagggcg aactcggtcg ccgtgagctc cacgggctcg
    10141 ccggcgcgcg tgacgcgcat gcgacccaca tcgaggacca ggtcaccggc acgcagctgc
    10201 tcgttggggg tcggcgcgcc accatggcga cgcaggacgg cgcggacgcg cgcgacgagt
    10261 tcgcgtgggc tgaagggctt ggtcacgtag tcgtcagcgc cgagctccag gccgaggacg
    10321 cgatcggtct cgtcgtcgcg agcagtcagc atgatgatcg ggagttcgcc gctgcgccgg
    10381 atggcgcggg tgacgtcgag gccgtcgagc ccaggcaggc cgagatccag gaccaccagg
    10441 tcgggccgct ctgtgcgcac ccgcatgagc gcctcgttcc cggtgcccgc ggtgctcacc
    10501 gtgaacccgg cggcagcgag gtagtcgacg acgagccgaa cgattttcgg ctcgtcgtcc
    10561 acgatcagga tcctggccat ctccggcgca tcctacgccg ggctcagccc gacgccgact
    10621 cccgacggtg gagctctcgg tgccactcct cggcgcgctc cgagcggcca ccccaggcac
    10681 cgccaccggg tccccggccg ggaccgcccc atcggcccca cccgaaggcg gcgcgcagca
    10741 ggccgatgat gaggaagaac ccgaggatcc agaagaagat cccgaagaag ccgaagccgt
    10801 agccccagcc gccgtgccag tacggcccga cccagcccgg agcatacgga gcgaccacct
    10861 caccggattc ggctgcctgc cgcgcggcct cggtgaagcc ggccgatacg ccggcgttgt
    10921 agacagcgac gccgattccg accgcgatcg cgatggcgag cacggcggcc gcgatccgcc
    10981 agaacccgct catgccgtca cctcgtccgc agcatcggcc gcatgaagct cgcggtggag
    11041 cttcgcgatc atggcgcggc gtcggtcgcg catcggcccc cggggtccgt ggcgcaggag
    11101 gatccgcagc agcatcacgc cggcgaggac cgccaggatc cgttgaacgg gtggagtgga
    11161 ggcccgttgc ctctcgtcgg tacgcatcgg ctcatcacct cgagattcct gcggcccgcg
    11221 cttcggaccg ctcgcacctc aaggtgattg tggaatgcgg aggaagtgtg aacgccggat
    11281 gggcgtgtga cgaacgctgg cgccgtcggg tcagcggcgg cggcgcgccc aggagccgcg
    11341 gtacgactcg ggatagtggc cacggaggaa ggtgtgctcg atgtggccga gggccagggt
    11401 ccggcccacc cgctcgacgg cggcctggct ggcgagagcc gctcgctcgt cgatcagccg
    11461 ctcggcgcga cggcggatga gtccggcgta cgcggcagca ccaccgatca gtccgcacag
    11521 cgacagggtc tgcgccagga ggaggtgcga cgcttccatt gcggcacggt agccgcaccc
    11581 gcgccccgtg gcactagccc gttcaggtca gcggcgggca ggcgatggtt cgacgggtca
    11641 ggaccgaagg tcgccgagcg tggcgaggtc cccctcgatc acctgccggt cggcggggtc
    11701 ggcgatggct gccgtcgccg cctcggccat ctcgcgccac tgcacgaacg cgtcgcggtc
    11761 cgcggccacc cggctggccc tcgccatcgc ctcgtaggca gcggccagca cccagtcgtc
    11821 gaccggtcct ccctcggcca gcgccaggca cgcccgcgcg tggtgcagcg cggcctcggc
    11881 ccgaccaagg acggagtaca cgcgcgcgca ctgccactcg cccaccgcgc ggcgctgacg
    11941 aacgtcgggc gggtcgaccc gcatccagtg aaagaccgat gcgtgcgccg cgtggatcat
    12001 ctccgcgtcc tggtccgcgg tccgatcctc ggtctccagc agcgtccagg tgtgattgaa
    12061 caggtcggcg gccaggcggc ggtggacatc ggcgcccacg gcggtcggct cggcatcggc
    12121 ggtcatggga acctcctggc tgagttggcg cttgacgagc ggcggtatct tgacgctccc
    12181 tccctcgcgt gccccgcgag ccacgataga cgtgcccgga agaacggtcg acccatgctc
    12241 tggcacggat tgatgaactg gttcgatgag gtcaccggcc gcgcgcgccg tcgccggctc
    12301 gaggaagagt tgacccgcgc cgatgccgag atctggcgcc tccgacagct cctggccgaa
    12361 gccacccgat cgccgatgga gttcgcgcgc atgcgggaac gcgaggtcgc gctgcgccga
    12421 ctgacccggc tgcgcatcgc gggagagggt caccgagaca cagaacggac gctgcgcgcg
    12481 ggctgacccg atcagcctgg acgccagggc cgatccagct tgacgtggtc gaccaggctg
    12541 atcgcggcag tcacccgcgc ggctgggagg ttaccgggcg gtcgagcagc aggagcacga
    12601 cggcgacgat cgcgacgatg ggccctgaga tggccggcgg tggcggcgcc tcgtcgggag
    12661 ccaacccggc cacgaggtag aagagcggca cgccggcggc ccacgccgga agggtccatg
    12721 ccgccagcca cgcccagctc tcgcgtcgtc ggtatggccc cacgatcacg gccgccagca
    12781 ggacaccgat gaggaccagg ctccagccgt tcactcgaga ctggaagtcg aagagccgat
    12841 acgccgccgg gccgtcctgc tggagctcgg ccaggctcat gcccgtcaat gcccggccga
    12901 tggcgggatc ggccgatgc
//
LOCUS       DASTYP010000064        45394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1643837, whole genome shotgun sequence.
ACCESSION   DASTYP010000064 DASTYP010000000
VERSION     DASTYP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 45394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45394
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643837"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..792
                     /locus_tag="VFV59_03140"
     CDS             142..792
                     /locus_tag="VFV59_03140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5324856.1"
                     /translation="MVDTTKTVGEHFDEWAPSYDAQIREMVPRYEEIHETLLALLALR
                     PPHRFLDLGAGTGYTLRRVLESFPETTALGLDVSAEMLARGRQRLEGLEDRVELRQTD
                     LVSPEIDGAYDAIVSILAVHHLYADEKRHLFSRIWEHIEPGGVFMLADYFRPARNRLS
                     ELYHLPAEPDPHEVEHDHPDTAADHLAWLIAAGFAAVDVVWKYDDVGVLVAWRDER"
     gene            complement(693..1508)
                     /locus_tag="VFV59_03145"
     CDS             complement(693..1508)
                     /locus_tag="VFV59_03145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5324857.1"
                     /translation="MSIEYERAFYDSDPAYDGRFVAAVRTTGIYCRPSCRARKPLPKN
                     VSYLPDGLTARVAGYRACLRCHPDAATTVVVRSVDTPIGPMVLGATDTAVVMAEFAER
                     RMYPAQVAGVRRRFGTVVDGASPLLDRAEAQLREYFDGERRQFDLPLDVPGSPFQELV
                     WRELQRIPYGETISYRELARRVGVPAASRAVGRANGSNRLAVIIPCHRVIAAGGGLGG
                     YGGGLDAKRWLLDLERRTVSAQRSSRQATSTPTSSYFHTTSTAAKPAAISQAR"
     gene            complement(1564..1971)
                     /locus_tag="VFV59_03150"
     CDS             complement(1564..1971)
                     /locus_tag="VFV59_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324858.1"
                     /translation="MADVQLHTLLLCDYALTAQDGKISAVGVFSQINVNRLPATHGRC
                     FIVAILEATPGEHELSFQVVAPSGTPTLQQAPRLRINVPPTATTANIVADLKGMRLEE
                     IGRYRIEVRSGDTVLGSSPFLVNLILRQPTGQA"
     gene            complement(2001..2339)
                     /locus_tag="VFV59_03155"
     CDS             complement(2001..2339)
                     /locus_tag="VFV59_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5324859.1"
                     /translation="MSFLFGPKVEEADVKQVVDALPEDGAVLVDVREPHEWRAGHAPQ
                     ARHIPLQQLPMHLDTLPRNAPVYLICRSGNRSHHAAAFLQRNGFERPVNVRGGMIAWE
                     RAGLPVERKG"
     gene            complement(2336..3739)
                     /locus_tag="VFV59_03160"
     CDS             complement(2336..3739)
                     /locus_tag="VFV59_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5324860.1"
                     /translation="MYLQQFFVKGLGHASYLIGDASGGVAFVVDPRRDVEDYLHAAGD
                     EGLRITDVLETHVHNDYVSGADELRRATDATVHASPTAQLTRPHQPMSDGDELRVGSL
                     RVRAVATPGHTPDHLAFLVADLSRTDEDWILLSGGALLVGTAARPDLLGGPEEARRAA
                     AVLFHTLQNRISPLPDWIELYPTHGAGSLCGSGIGGKRWSTVGFERRHNPALNQPDAD
                     AFTRFILADQPSVPAYWRRMRPTNQAGAESLAELATPRAMTADELSHAVGHGASVIDA
                     RDPDLFAAGHIPGSLSIGLSDTFGTWTGSMAAIDRELVLVLERPEDLDPALAQLRRAG
                     FDRVVGYLVGGFDAWHGEVETLRQVRSQQVPAEPGTVLDVREPSEWRDGHVPGALHIP
                     AAQLPNRLDEVPTGPILVVCAGGYRSSIAASLLAREGREDVAHVIGGMDAYRSAGLPL
                     ETDEQEPVRQPVEVSAR"
     gene            complement(3833..4303)
                     /locus_tag="VFV59_03165"
     CDS             complement(3833..4303)
                     /locus_tag="VFV59_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015033625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5324861.1"
                     /translation="MTDEPAPLEYRRHLRVSAYAVCVHDDAMLLTRITPGYTVESDGK
                     WTLPGGGLDHGEDPRDAVLRELDEETGLIGEVAELLAVDSLAAKFPSAQDGLPTDFHG
                     IRILYRVRITGGELRDEFEGSTDAARWVPLAELPALPHVSLVDVAAELVGAVPD"
     gene            complement(4300..4662)
                     /locus_tag="VFV59_03170"
     CDS             complement(4300..4662)
                     /locus_tag="VFV59_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5324862.1"
                     /translation="MPEPAMISRRFDDPDEHREFPLGRFDLVQIGGLSVGRAEYEPGW
                     RWSEHVGAALGQSACEVAHVGLVLSGRNRIEMTDGRVIEVGPGDLFEIGPGHDSVVVG
                     DEPYVSLHFLGADQYATK"
     gene            complement(4677..5144)
                     /locus_tag="VFV59_03175"
     CDS             complement(4677..5144)
                     /locus_tag="VFV59_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5324863.1"
                     /translation="MILYFLRHGRAEQGSDDERRDLTADGEAALLAAGPLWHRLNLRP
                     DAVISSPVLRARRTAEIAAEQIGRTPILDDRLRPGADWGDVARALATHAGDTRRVLFV
                     GHEPDLSTIICHLTNASSVRMRPGGLACLEFPGIPEPGAGEIAWLIDPDLYTE"
     gene            complement(5141..5581)
                     /locus_tag="VFV59_03180"
     CDS             complement(5141..5581)
                     /locus_tag="VFV59_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5324864.1"
                     /translation="MTDATAIPVNMFDNDRELMVVAPMPGVAPEDITIDVTDDGHLTL
                     RAAQHGEGQERIDYLLREWAYGPYERTVELPCAVDATRANVSYGNGVLAITFPKVGAT
                     AGGRVLVRRTGQARGVTAGHAGTRGGDEDDPTPNDDSSATTVVV"
     gene            5675..6052
                     /locus_tag="VFV59_03185"
     CDS             5675..6052
                     /locus_tag="VFV59_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5324865.1"
                     /translation="MPEHPFVIRAGEGQAGPPTAGMERRTLVERPDAWAGWVRTDAGV
                     AGGWHHHGDHDSYIYVLRGSLTIDFGPGGRESIAAGAGDFVFNPAGLVHRETTSADGD
                     AEVFVVRVGDGPHLVNVEGPDPG"
     gene            complement(6287..7513)
                     /locus_tag="VFV59_03190"
     CDS             complement(6287..7513)
                     /locus_tag="VFV59_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEU5324866.1"
                     /translation="MFYVQRGEVPHKRHTQFRKPDGSLYAEELFGVEGFSGRASLLYH
                     HTPPTQTHQIEKVRDVVIEQSPDEASGAHRHRLVNTKDLEARGDGVSGRVPLFYNADV
                     TFGVVLPSEPMERYYRSGEADEMYFVHEGTGVLETIFGPIPYGPGDYLVIPIGTTFRL
                     RPDAGVPQRMLWLECPSNIEPPRRYRNEFGQLLEHSPYCERDIRPPDEMAPDLSIGDY
                     VVEVKTRGRVTAYHYRHHPFDLVGWDGHLWPFAFNIGDFEPITGRVHQPPPVHLTFQP
                     RNFVVCSFVPRKFDYHPLAIPAPYNHSNINSDEVIYYVAGNFMSRRGVDISSFTIHPA
                     GIAHGPHPGSVEASIGKEATEELAVMIDTFHPLHLTRQASELEDDRYPYSWLPPEDGA
                     EHARELSERGPEAFPD"
     gene            7559..7978
                     /locus_tag="VFV59_03195"
     CDS             7559..7978
                     /locus_tag="VFV59_03195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5324867.1"
                     /translation="MIIDPGTEKGARVDARLRSELVAWLVTVSADGTPVPTPVWFWWD
                     GATILVYSQPDRPKLRHIAANPRVALAMRTDEHGDALIVITGRATVDAAAPRANELDG
                     YLVKYDASIARLGATPAEFGGEYSVPIRITPTRLRAW"
     gene            complement(7988..8365)
                     /locus_tag="VFV59_03200"
     CDS             complement(7988..8365)
                     /locus_tag="VFV59_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5324868.1"
                     /translation="MSSDARILVVEDDDTLRETLAEVLLDEGYEVRVAGHGREALAVL
                     TSWVPEVIVLDLMMPEMDAFEFRRHQHDHELAPQARVMILSAARDLEGAAENIRADAW
                     LAKPFNLAEMLDAVDRLVAASAA"
     gene            complement(8362..9549)
                     /locus_tag="VFV59_03205"
     CDS             complement(8362..9549)
                     /locus_tag="VFV59_03205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU5324869.1"
                     /translation="MSVTQEDPRPEPDELAGVPQLRGELAFRLLVDAVQDYAIFLLDP
                     NGHVLTWNSGAERIKGYSADEIIGQHFSVFYVPEEREAGRPMRLLGLAAEHGRYEDEG
                     WRVRRDGSRFWADVILTALRDDQGTPYAYAKITRDLTERRAAEEQQRALLVEQRARAA
                     AEEALRARDRFLSIAAHELKTPVASLQLSAESLVRARDADRLDDERLRTGLKRMTKAA
                     DRLGDLVRELLDVSRLAAEHAPLQLSPTDLAALVRDVLARFEEQGETHRIRFTGPDVL
                     WIPGDVARLDQVVTNLVDNAMKYSPPEAPVDVTLAEEEAGAVLEVRDRGIGLDEASVD
                     RIFEPFGRGENAEHIQGMGLGLYISQQIVARHGGTIIGAPRSDGHGALFRVTLPRNEG
                     VQR"
     gene            complement(9603..10187)
                     /locus_tag="VFV59_03210"
     CDS             complement(9603..10187)
                     /locus_tag="VFV59_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU5324870.1"
                     /translation="MSSGPSRRRPLVGAQIRRRRRDRGLTLARVAAVTGLNIGYLSQV
                     ENDKASPSLETLAALAEALDVPIAWFLLDQSVAPRVVRAAERPSREVPSTDGELSQVD
                     GGIARDVAIFEATMPAGAHTGFHQHPGDEHHVVLSGRVRFLQGEAEVEAGPGDYVLLD
                     GTLPHDAQVIGDDPARLLIVYPRGGHSELTRAEP"
     gene            10332..10946
                     /locus_tag="VFV59_03215"
     CDS             10332..10946
                     /locus_tag="VFV59_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEU5324871.1"
                     /translation="MKVAIIGAGNVGKALATSISRAGHEVTISASTPESALEAADAVG
                     VTAAESNVDAAQQAGVIILAVPYVGAGPEVAQEIRDAVAGKTVVDATNPLKPDYSGLA
                     TSGTSAAEEFQKLLPDARVVKAFNTIFAANQASPSPEVDAYVAGDDGEAKRQVIGLAE
                     SLGFTPLDVGPLSAARTLEGMAYVNIGLNAANGWSWTSAWKLER"
     gene            10949..11425
                     /locus_tag="VFV59_03220"
     CDS             10949..11425
                     /locus_tag="VFV59_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5324872.1"
                     /translation="MAVTRLNHAVLFVRDLDAAADFYRRAFGFVEVDRPSGMRAAFLR
                     AAAGDNHHDLGLFEVGGSAGRPSSGSTGLYHLAWEVDTIESLAEMAGTLRELGALTGA
                     SDHGVSKSLYGRDPDGIEFEVMWRVPQEAWGRFAEQAVVMPLDLDAELKRWSAASV"
     gene            11442..13376
                     /gene="nhaA"
                     /locus_tag="VFV59_03225"
     CDS             11442..13376
                     /gene="nhaA"
                     /locus_tag="VFV59_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEU5324873.1"
                     /translation="MKETGGRGTRYHGETAAAAPRLAALRSFLQTEAGGAVVLLAATV
                     VALAWANSPWRDAYHALWATEVGLTIGDWSLRYDLGHWVNDGLMVIFFLLIGLEVRRE
                     FDMGEFRERRRVAVPVLAALGGMLLPVAIFLFLAPDGDAARGWAMVMATDTAFAVGVL
                     ALVGRRSSFRLRTFLLTLVIVDDVAAVVVIALFYSTNVELLALGIAAALLVLMAVLRR
                     VGVMRSDVYVLLSVAIWLATLQSGVHPTVAGVAIGLLTSAYPPRRTSLEEATGVTRLF
                     RQRPTPELALEAARRIRTSLSPNERLQHGLHPVSSFVVVPIFALANAGVDLGGDILGR
                     VLGSPLVLAIVLGLVAGKSVGIPIGSWLATRRWLGGQALAVGWPSLVAASSVAGIGFT
                     MSLLIAELSYTGETLDEAKLGILLASSVAAVLSVGMFHAVALLPRELLSRAEAMTSRP
                     LSDLTDAVDPVRDHVRGPLDAPVTLVEYGDYECPHCGRVAPVIRELLARFEGRLRFVW
                     RHLPLTDVHPNAALAAQAAEAAGAQGRYWEMHDALFEHQDALDPVSLRGHAERLGLDV
                     AAFEADLHDGRHAGRVAQDVNSAEEAGVAGTPSFFINEVRYRGSYDLDSLDRLVRRVL
                     RLVESRATAAAATTEERAAD"
     gene            complement(13373..13858)
                     /locus_tag="VFV59_03230"
     CDS             complement(13373..13858)
                     /locus_tag="VFV59_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5324874.1"
                     /translation="MSASVDNLLARMADAERRLVEHADAPIPAGLTDPDPGGEERWEA
                     GQVWAHLAEFPDYWLAQAERVIGGASDSPVPFGRTKADADRIAAIERDRHTDPAALLA
                     RVRESLARVTDAARGWPPEAWRRVGVHPTLGEMTVERLVEHFIAAHLEEHADQLDGLA
                     R"
     gene            complement(13855..14847)
                     /locus_tag="VFV59_03235"
     CDS             complement(13855..14847)
                     /locus_tag="VFV59_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU5324875.1"
                     /translation="MKIAHVRSRHAPAGAAWRLAAALDAEGARWHDLEEARRSLVAAD
                     PRRAHNSVLFRQPVTTLDEHLARGLRVDALADLLDAEPEEPMDAADLAFGPPILEPPT
                     FRDFYAFEQHVGTMWKRRDMEIPEAWYRLPIFYFSNISEMRGPGEPVWAPRGSSELDY
                     ELEVAALVDTPVRDLEASRGEEAIGGYTILNDWSARDLQREETTVRLGPAKGKDFAST
                     IGPWLVTPDELADARRETGYELTMTASVNGEELSRGTWSSAHFSFGQMIERASADVRL
                     RPGDLIGSGTVGFGCLLEVRDQKLKRYLQPGDVVTLAIERLGEQTTTVVERPGR"
     gene            complement(14875..16218)
                     /locus_tag="VFV59_03240"
     CDS             complement(14875..16218)
                     /locus_tag="VFV59_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEU5324876.1"
                     /translation="MRWSSLTDRLLGWLLPVLAVVAEGAFLGVVYVAIETTLDARAPL
                     LGTLELSVAAGITALAAQRRWVQPDEEPTSFVLLLLGLGIVGWLWSSEARDLLLAGNP
                     IDALLHHPGGWLMLVAGMRGVGRAELTDDRALTRLVLVGLPALAAPWAFGQFSSPALR
                     PVFTDAAFVSSLTFLTAGFTAAGLARLQEIGRQTGVDWRRDRSWLGTVLAVLALVVGI
                     GLPAAWLLDLPADAVARGILEPVLGILGYILVTFAAIAALIASILARGIRSFGFELPP
                     PMTPDQIARLQEVPKFTVEQLRGPIVIVIVVWGLLIVLGVVLLRVWMRARARRGGGSA
                     NEERSIRIPPGLFRLPRVGRAQPARPAAPVIARDAVTAYLAALEAIREQRPTAARQPN
                     ETPRAHARRASLGPELAALQADYALARYADRPITAAEHRRAIGRWRRLRDRLRPG"
     gene            complement(16196..17431)
                     /locus_tag="VFV59_03245"
     CDS             complement(16196..17431)
                     /locus_tag="VFV59_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5324877.1"
                     /translation="MAAGWTLFGLGLIVIGAAIGAPGLALVGGLTLLSRWLITLWSRF
                     GLESIAFDRRLATDRAVWGDQVPMELEVWNRKLLPVPLLTADDHVTDTLRVAGHTLGP
                     SERPGRGTLRNAWSLLWFERVVRRLTIDARRRGVFVLGPVRLTVSDLFDRGTRSDERE
                     LPAELLVRPRSVPVRSMERAMAPLGSHRVPASLFTDPARFAGVRPYQRGDPMRRIHWR
                     ATARTRAPVSRRYEPVRERQVLLAVDLQTVPGAYWLMLFDDEQVEALCVAVSSLARRI
                     LRDGAACGLLLGTQLAGGRRWAYVPPTAAPGQLGRIDDVLARVQPIVSLPFETLLDAV
                     PRLFVPGGSVVVLSARDPAPYLDPMRRLRGSGYTVDHVALGPEREAARGTARAAGLRA
                     RTGSLDPGWRDAHALVLAD"
     gene            complement(17441..18397)
                     /locus_tag="VFV59_03250"
     CDS             complement(17441..18397)
                     /locus_tag="VFV59_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5324878.1"
                     /translation="MSDPHAGARLWAALRTSVERAVVGADEPLRLLTVTLLAEGHALL
                     EDVPGVGKTLLARAFARSLGLSFGRVQGTPDLLPTDVTGSSVLADGAFRFIPGPIFTN
                     VLLVDEINRATPRTQSALLEAMQEGQVSVDGETRPLPAPFLVLATQNPVELEGTFALP
                     EAQLDRFLVRIAVGYPEEDQERAIARRYREAAEPLEAIGPVADVAELPTLRATVRSIR
                     VSDEVERYVVRLVRATRDHADLRLGASPRASVALYRAAQAAAFLEGRDYVLPDDVTAL
                     VEPVLTHRLLVDLDRELRGATAAAAVREILESVPVGVGAEAG"
     gene            18464..19531
                     /locus_tag="VFV59_03255"
     CDS             18464..19531
                     /locus_tag="VFV59_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEU5324879.1"
                     /translation="MPPPTPLIFSEAHRAHDGLVELFAGREIPCWESPARAETILAAL
                     LETGDYALEPPEEHGPDAIAAVHELELIDLVESAWTDAVADGHDPARSLLPDTFLLAA
                     YRGSMTLEQLPAQRHLRLGAYCFDTATPIVAGTAAAARDAVDVALTAVDRVLEGGARL
                     AYGLCRPPGHHAGRNLIGGYCFFNNAAIAAQSMRDRGAERVAILDVDFHHGNGTQQLF
                     WERGDVLYVSLHGDPRVSYPYYSGFATERGAGDGEGATRNFPLPPGTDGHAYLATLDE
                     ALATIRAFDADAPLVVSLGFDTYETDPISSFALRTDDYARMGARIADLGMPAVVLQEG
                     GYAVDAIGRNAVAFLGALRDG"
     gene            19528..20727
                     /locus_tag="VFV59_03260"
     CDS             19528..20727
                     /locus_tag="VFV59_03260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324880.1"
                     /translation="MTGSTPRPALWPAYLATFTFMLGGWATSIAVPLNVVLLGGSLAE
                     AGVLAAIRFGLQAFLQLPFGAVIDAWGTRRVLFIATAGNALVNVVPVLAVVTGSILPL
                     YAWAVLSGVSASLFLPSTSAYIAASAEPRTRGSAFGWLTLFTHTGVASGPAVGGVLWD
                     LAGPAPTYVVAGLLGAIAVVAPFFVPAVVPQRVRLTRLPGMVAEVARQRAIVGSWVAA
                     LAIGLPWGAVSGLFPLFGTGIGLTAGTVGLLMASQSVANGASRVPLGRTIDRLPIPPI
                     VAALTAAGYGVVIGLLGLQGNVVLILVVLVAGIVMLAFTLMLVQVTISEVARPEVRAT
                     GLGGYGTALSAGLGLGPALGGAIADAAGFGWAFATIGVAGVAVAAVAALVLIGTRRRP
                     AVVVFDA"
     gene            20765..21787
                     /locus_tag="VFV59_03265"
     CDS             20765..21787
                     /locus_tag="VFV59_03265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004691143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5324881.1"
                     /translation="MKRIGFLSFGHWSDSPYSQVRSGSDALIQSIELAVAAEELGADG
                     AYFRVHHFARQLASPFPLLAAVGARTSRIEIGTGVIDMRYENPFYMVEDAGAADLIAG
                     GRLQLGISRGSPEQVIDGYRHFGYVPGEGETDADMARRHAELFLKLLDGEGFAQPNPR
                     PMFPNPPGLLRLEPHAPGLRDRIWWGAGSTSTAVWTAQLGMNLMSSTLVFDESGEPLH
                     VQQRRQIDAFREAWKEAGHQREPRVSVSRSIFAIVDDRDRAYFGRGQQEHDQIGQIDE
                     KTRAVFGRSYAAEPQALVDQLREDTAIQAADTLLLTVPNQLGVDYNAHAIESILKFVA
                     PELGWR"
     gene            complement(21809..23803)
                     /locus_tag="VFV59_03270"
     CDS             complement(21809..23803)
                     /locus_tag="VFV59_03270"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5324882.1"
                     /translation="MAASSLAESARSALDRHAWREAYDLLAEADRAGSLDAQELELLA
                     EAAWWLGMLPTAIEVRERAYAAAIKGGETETAVLVAVWLARDHLFRNALSVASAWLSR
                     ADRLLEGVPENPGHGWVAATRGFHDALAGAPERTLEEATRAQEIAARFGDRNLEALAM
                     SERGAALVALGRLEEGLALVDEATVAAVGGELDPATAGGICCTTIESCATIGEWGRAV
                     EWTEAQDRWCRREGISGYPGMCRLFRAEIKRLRGSWLEAEAEARRASDEMMGYIPTAV
                     GGALYQIAEIRLARGDLPAAEEALQRIHALGTDPEPALSLLRLAQGNAAAAAAGIRRA
                     LDEPTRTPAWRGPPGSDVYRMPLLAAQVEIALEARDVRTARAAADEMTATAGRYGTLQ
                     LRATALAATGAVEVAEGATANGTRTLRSAVQAWLEFGSPYEAARARLRLAEAYAAAGQ
                     PDDALAEAESARATFEEMGATPGLRRTEALIAALPAEARGAGATSSSARRDVRAFVFT
                     DIVDSTRLAELLGDEAWTRLLRRHDETLRAVVAAHGGTEIKATGDGFFLAFEDPRAAI
                     DAAVEIQRRLVEQRATHGFAPEVRIGVHQAEATRSGLDYIGVGVNQAARIGAAAAGGE
                     ILASAGTVAAAGRPVPEAERRTIELKGIEQPVEVVSIAWR"
     gene            23902..24696
                     /locus_tag="VFV59_03275"
     CDS             23902..24696
                     /locus_tag="VFV59_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5324883.1"
                     /translation="MRQRREPRAGAPRETSWERVATWYDGWVGDAGSTYHRELAIPAT
                     LDLLQPQPGEHILDVGGGQGVLAPYLVEAGARVTVVDASAKLIAAAKRRHARLRDARF
                     LVGDARRLPAVSGLEPHACDAATFLLSIQDMDPLDDVMRGLDWALRPAARVVLLMTHP
                     AFRQARHSGWGYDEGRKLTYRRIDGYLTELAVPMKSLGGGLPTRSFHRPISRYVNALA
                     DFGFATDAMLELPDLPPDRRPGKAARGDARANAEIPIFLGLRAVRR"
     gene            24768..26102
                     /locus_tag="VFV59_03280"
     CDS             24768..26102
                     /locus_tag="VFV59_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5324884.1"
                     /translation="MLTTSPRRPPAAAAAVLLAVLLLFGALTFVAGLAVGGSGEGAAA
                     ASPSSSGAVSSDAGVPSSSPKPDVAVETVTCAEPTEAWAALCDAYGQIREEYVDEEQI
                     TDEELIDGAIRGMVEYGLGDPYSGYLTPGQYGEALQDLSGEFSGIGAEVGMENTANPD
                     DLAACTVITETCAVVIVAPIDGSPAEAAGLQPGDRILEVDGQTTVGETLSSLVYVVRG
                     EEGTDVTLTIQRGEDTFDVTITRGVIDLHEVSSELLDGGVGYVRLSSFTDRSTDLVRE
                     AMQSLLDDGATSFVLDLRGNPGGYIVAAQGVASQFVPEGELLFTVESDGNVREWRAES
                     GVAQSSDIPVVVLVDAGSASASEIVSAALQETGRATIVGEPTFGKNTVQIWNDLPNGG
                     GLRITTDRWFTPDHNSVAPDGVQPDLVVEGPPDATSAADPQLDRALEILRGG"
     gene            complement(26314..27297)
                     /locus_tag="VFV59_03285"
     CDS             complement(26314..27297)
                     /locus_tag="VFV59_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU5324885.1"
                     /translation="MGQGVLMGEPHIVSVEEIREAAERIRGVATRTPLLRWDDTTWLK
                     PESLQPIGAFKMRGAYNKIASLSDEERGRGVVTYSSGNHAQAVARAARLLGTHAVICM
                     PREAPAVKIEGVRRDGAEIQFTGMDSAERHALALQLVEERGLVMVEPYDDAAIIAGQG
                     TCGLEIVEDLPEVTSVLVPVSGGGLSAGVATAVKALAPSARVVGVEPEFAADAMESLE
                     SGRLVAWTAEQTGRTMADGLRTNSLGRLPFEHLRRFLDGIVTVSEDEIADAVRQLARR
                     ARLVVEPSGAASMAAHLNDRLPRTDDDVARVIVISGGNVDPDLYLRLLSGG"
     gene            complement(27279..28730)
                     /gene="proS"
                     /locus_tag="VFV59_03290"
     CDS             complement(27279..28730)
                     /gene="proS"
                     /locus_tag="VFV59_03290"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5324886.1"
                     /translation="MGSRATRADRLPSQADDFPGWYAEVVRQAELAEHGPAKGSMIIK
                     PHGYAVWELTQRALDDRFKATGHKNVYFPLLIPMGLLDREADHVEGFAPQVAVVTRGG
                     GQELEEPLAIRPTSESIIWSTYKRWIQSYRDLPLLYNQWGNVVRWEMRTRLFLRTSEF
                     LWQEGHTAHASAAEATEEALRMLEVYREVSEDVLGIPVIKGRKSPGERFPGAVETYCI
                     EGLMRDAKALQCGTSHFLGQNFAKAYDVTFLNADGVQEHAWATSWGFSTRMVGGTIMA
                     NGDDAGLRLPPSVAPVQVVIVPIYRSDQERSAVLSVADALRADLAGRGLRVRLDDRDQ
                     HRPGFKFAEWELKGVPLRVELGPRDVAANRAVVASRVSGAKEEVGLGVLAADMAERLA
                     SVQRELFADALAFREANTHEITSFGDFAAGVEEQGGFWVGAWCGDAACEAEVSAKTKA
                     TIRFLPLEPRHPGAACVHCGAPGAELATWARAY"
     gene            complement(28777..29862)
                     /locus_tag="VFV59_03295"
     CDS             complement(28777..29862)
                     /locus_tag="VFV59_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEU5324887.1"
                     /translation="MAIAVIGILVWTFRAELAPFFGVNLDAGSGGRVELTLPEGFSAS
                     VFAEGLDNPRFIEVGPDGTLFVAERGADRVVALPDANGDGRADEVVEVGMGYGRAQDL
                     AFTDDGRLLVAGESTIWEVTLDESREADRRPLVDGLPTGGFHSTKSLAVLPDGRVLVS
                     IGSSCNVCDEEDERRASVQVLEDGQLRPFVIGLRNAVGLWVDPATGQPWATNMGRDLL
                     GDDRPPETLYRLIEGTDAGWPRCHAGTLRDPEFGTDVTACDGVLQPALTLPAHVAPLG
                     LVEWQDHLVIALHGSWNSSVKVGYALWWLPWDDGPAGDVAPLATGFLPDGAEDALGRP
                     VGLAVGADGGLYVSDDKAGFVYRITAP"
     gene            complement(29943..31109)
                     /gene="hppD"
                     /locus_tag="VFV59_03300"
     CDS             complement(29943..31109)
                     /gene="hppD"
                     /locus_tag="VFV59_03300"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEU5324888.1"
                     /translation="MTDTLTRGAANQAGAIVNPNAAAEAQDFLPLKGIDHIEFWVGNA
                     RQAAHYYRTLWGFTPVAYAGLETGVRDRSSYVMVQNDIRFVFTAPLSPDGEIAEHVAK
                     HGDGVKDIAFAVDDVTSAWEETTKRGAVSALEPTELEGQKGRLRKSAIHTYGEVIHSF
                     VDRSDYHGTFAPGYHRVKAPPPAATGQSLLEVDHCVGNVELGDMNRFVDFYKDVLGFA
                     QLIHYDDKVIHTEYSALMSKVMQNGNGRIKFPINEPATGKKKSQIQEFLDYYLDAGTQ
                     HIALRTEDIVGTVRALRERGVEFLGMPHAYYEQLPDRVGDVGVPMDVLEELGIEADRD
                     EEGYLLQIFTRPVQDRPTFFFEIIERHGSRGFGVGNFKALFEAIEIEQARRGNL"
     gene            complement(31141..31644)
                     /locus_tag="VFV59_03305"
     CDS             complement(31141..31644)
                     /locus_tag="VFV59_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324889.1"
                     /translation="MDPVLLLVQTLHILAGAVWLGGSVFMNVVVLPALLALPAVEQRA
                     SARRVIFGPERMMIGAALLAAVLGIVRGTAYGPIDSVDSLTTPYGIAWSAAIAITVMV
                     FAVGGTVTSRTARALLDDDRYWTSGPRGDGSEARAPLVTRLRLAFRLELIGILAVFTL
                     MPLLRFL"
     gene            complement(31650..32381)
                     /locus_tag="VFV59_03310"
     CDS             complement(31650..32381)
                     /locus_tag="VFV59_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5324890.1"
                     /translation="MPQIHLRAEEGDYAPLVLLPGDPNRARRISERFDDGSARQVNDH
                     RGLLGFTGTYRGTPVSVQTSGMGTPSISIVVEELLRLGARRLIRVGTCGGIATGLKTG
                     DLVIATGACPVDGATRTYLHGEAYAPVADFELTRALVDAAGTDGVPARTGLVASVDVF
                     YNPDDDYARRWRERGVLAFEMEASALFFLAARSGAQAACALTVSDVLSEEVTTEESYL
                     PLEELDRAIDRMIGVALNAGTFGGR"
     gene            32447..33349
                     /locus_tag="VFV59_03315"
     CDS             32447..33349
                     /locus_tag="VFV59_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEU5324891.1"
                     /translation="MGKASKRSRDREDVGQTARQLLRAVGGGLIIGLPLLFTMEMWFH
                     SFLLSSWKLVVLLLIAFLVVIGYSALSGFRHERTWGELLVDSVHTMGIAAVVSALALL
                     LLGRIDPDTGLRDAVGKIALQVIPVAFGVSLAGAQLAGGDGEDEEDQGSSATDGGGQL
                     GAFARLFVGAGAALLFALNIAPTEEPMLLGVEAEWWLLLLVVAASIAMTLAIVFYADF
                     RGGRSPEGGDSILDHPLTETVAAYAVSLAVSLLLLWSFGRTDGASWSAIVGQTVMLGF
                     VAAFGAAAGRLLVGSGSASSAGAT"
     gene            33354..33728
                     /locus_tag="VFV59_03320"
     CDS             33354..33728
                     /locus_tag="VFV59_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324892.1"
                     /translation="MRQNWLEWVALAISVVAIVTVVGFLVVDGIRDEGRPPMPVAEVR
                     MAEAYQAETGWLVPATIRNDGDQAGEAVQLVARASVGGSEEEAELTLDYLPSGSELDV
                     LFGFSGRPDGEVTVTVRGLRLP"
     gene            33733..34560
                     /locus_tag="VFV59_03325"
     CDS             33733..34560
                     /locus_tag="VFV59_03325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5324893.1"
                     /translation="MTRWADDLAFATDLARRAGAVLTDHYERLERIDYKSKRDVVTNA
                     DYASEHLVIDAIKREHPGDAILAEESGEHSGALRDDGTHNGRCWVIDPLDGTVNYANG
                     IPYYCVSIGLVVDDRPAVGVIFDPARGDLYTATADGPALLDGDPIEASTKETLTDYVV
                     SLAVIGRGGLARERRIAPRIRIHRRMGSAALSLAYVANGRFDAFVQNGGLSPWDVAAA
                     GLIAERSGAVVTSIDGGAWWNPKVRGPRTSVVAAPAAQHQALLELLGSLKTTVQTRR"
     gene            complement(34557..35228)
                     /locus_tag="VFV59_03330"
     CDS             complement(34557..35228)
                     /locus_tag="VFV59_03330"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU5324894.1"
                     /translation="MERILRAHAGWEDEVAEALRLVARPIPLDERLLPRTLDGQTTRR
                     RYDRSGFPPARPAATLLALYPDEDGDLVVPLTIRRSELRAHAGEVSLPGGAIDAADAS
                     AEAAALREAWEEVGLEPSLVRVLGRLDPVWIPVSNYELEPFVGAVARRPPLVPHDAEV
                     AAIVELPLRALFDPAVLADAEFDARGVTVRAGAYRHGELVVWGATALTLGMFAHVLEA
                     AAGTP"
     gene            35227..35667
                     /locus_tag="VFV59_03335"
     CDS             35227..35667
                     /locus_tag="VFV59_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324895.1"
                     /translation="MTQAHLDRDSRVRSALYRTRILVNRSPHLTDATRKEANRALDML
                     EGQLGLNQVNPVDAARGLELLNRAHSSLLFGLLRNTDVADRFAPALRRLGLRGITDRL
                     DEVPHSVMALPMPGPSGGRRHRDDLPPDERLDAHGNPLPPPPGY"
     gene            complement(35661..36488)
                     /locus_tag="VFV59_03340"
     CDS             complement(35661..36488)
                     /locus_tag="VFV59_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324896.1"
                     /translation="MADLVVERLVHVYASSGVRAVDGVDLRIGPGERVAVVGQNGSGK
                     TTLVRHLNGLLRPTEGRVTIDGRDAATLTVAQLARHVGLVFQDPDRQVFAGTVHEEVA
                     FGPRNLGLRGAALHAAVEEAVVASGLTGDERTNPYDLGQSRRKLLALASVLAMRTEVL
                     VLDEPTTGQDRRGVDRVREVIHAAAAAGRTVLAISHDMAFVAETFERVVVMRSGRIVL
                     DGAPSEAFAAAAWEALRSTYLEPPLAAVVGERLGVGSTPTDAALVEALATREASAGQ"
     gene            complement(36481..37293)
                     /locus_tag="VFV59_03345"
     CDS             complement(36481..37293)
                     /locus_tag="VFV59_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324897.1"
                     /translation="MAISLRGVGYRYAGAQQAALLDVDLDLPDGAVVGVAGASESGKT
                     TLCLVVSGLAPRTVGGRIRGTITLDSEDVDAWPMHRLASRVGIGFQNPTTQLSQVAGT
                     VFEEVAFGPMNLGVPRDEVAQRTWEALAALRIDGLAERDPARLSGGQQQLVAIAGLLA
                     MRPEHLVLDEPTAQLDPAGTQLVGDAIQRLAGDGASILVAEQKTDLLAAVAGRVVVLD
                     GGRVVLEGDADAVLSDPRLAELGVPEPSAVRLRRAVVDAGVAPDRVEAAISG"
     gene            complement(37293..38117)
                     /locus_tag="VFV59_03350"
     CDS             complement(37293..38117)
                     /locus_tag="VFV59_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU5324898.1"
                     /translation="MAERNELTDPSLSSRGVPDFLGRPGPSAFHRLNPLTKAVLATVT
                     AVAAVVLGGLVGPILLVTVTVLLPGLLAGVLGRLLRTALLLALPIAVSAFLVNLFFFP
                     GGTTELLRIGPVVATAEGLAFALEILVRILAISGAVTLFYLTTRPADLVVDLERRGVS
                     SRVGFVANASVQTVPAMVERAAQITAAQRARGLDTEGSVWRRVRGIVPIVGPVILGAI
                     AEVEERTMALEARGFTRPGRRTLLWAPPDTGWERAVRWALLFALLALIIVRVAGIA"
     gene            complement(38096..39193)
                     /locus_tag="VFV59_03355"
     CDS             complement(38096..39193)
                     /locus_tag="VFV59_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324899.1"
                     /translation="MLVRWIAALVAFVAVILIGVVTVGNFDTGIDPGNFFDTEGSDSF
                     AFIGAIALVVAFLVYAAIEYAQTGGFGSIARQFDTRTIVLMPIAIAINVILGATVANA
                     LKIPVYLDSIGTILVGALAGPVAGAFTGFLTNILWTYVLPPPFQNGPAAAFALVAAVI
                     GVLAGFAGRAGWLRPRPNRSGTELLIGGLVALAVVVGLAVYAYTRFYGSTFNFFIPDV
                     EVEPLFVVLGWLVGLIFVGFVVGFGYLLVARRDLGVAYVVVAGMVTGVIAALISAPIS
                     ANVFSGVTGGGTDFLVAAFRQAGSDLQTATFQQGLLSDPVDKLVTFFVVYLVLNAMAR
                     RTKARFPQGEQLLEAGDTGGIEEGEWRSATS"
     gene            complement(39226..40218)
                     /locus_tag="VFV59_03360"
     CDS             complement(39226..40218)
                     /locus_tag="VFV59_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU5324900.1"
                     /translation="MDADAARSGETAAPRPLILDVDTGIDDMIALLIAATSPELNLRG
                     VTCVAGNVEIHHVARNTGKILRMVGRGDVPVSIGAARPLQRRLRTAPDTHGPTGTGYV
                     ELRGEDRQLGTTEYTAIPASRYLGAHVGRHPGEMTIVALGPLTNLAAAVQHGVDLAGS
                     TREVIWMGGTLEERGNTTETGEWNACVDPEAAEVCLAAGALHRIFPLDATQDVVFTMD
                     DLRALPDTHLAGIVRDAVRFYVAFHQRADGIDGAYLHDPVVLVGSLLRPDLVTDSVSE
                     QLACDTSSDPYIAGSLYRSEDGRRPVGVATAIDGEGMKAELLARLARAVSAPYS"
     gene            complement(40226..41131)
                     /locus_tag="VFV59_03365"
     CDS             complement(40226..41131)
                     /locus_tag="VFV59_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU5324901.1"
                     /translation="MPDRDVRIFEVGPRDGLQNEATPIPTDAKLRFIDLLADAGLREI
                     EATSFVSPKAIPQLADADALMARLDRRPGVRYPVLVPNERGLERAEAAGVEAVCVFTA
                     ASEPFAKANINMTIAESIDAFRPVIDRAKERGWWTRGYVSTAFGCPYQGRVGEAAVVG
                     VAQQLLDLGVGELSIGDTIGVAGPADVRRVVGALLNAGISPDRLAMHFHDTRGTALAN
                     VAVAMDLGVRCFDSSTGGTGGCPYAPGAAGNLATEDLVYLLDREGCRHNVDLDALLVA
                     AREISDTLGRPLASKVGQAGGWRVP"
     gene            complement(41124..43154)
                     /locus_tag="VFV59_03370"
     CDS             complement(41124..43154)
                     /locus_tag="VFV59_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016851703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5324902.1"
                     /translation="MADEELTPSQVARELGVTARTVQRWISSGRLPARQVGARVRVSR
                     SSLSAVGTAGPPASTTPIRSLLIANRGEIAVRIARTAHRLGIRVIGVHAADERPAHGA
                     DESHEIDSYLDGEAILAVARRAGADAIHPGYGFLAENAAFAESVDRAGIRWVGPPATA
                     IAAMGDKAAARQRVSEHGVPTIPGYDGEAQDDTTLAEQADRIGYPLLVKPSAGGGGKG
                     MRVVRTADELSEALAAARREAHRSFGDDRLVLERYLAGSRHVEVQVLFDAHGNGVHLG
                     ERDCSTQRRNQKVVEEAPAPAVDAARRERMGTAAVAAAAAVGYVSAGTVEMLLADSGE
                     FFFLEMNTRLQVEHPVTEEVTGRDLVADQLRIAQGATLDELGLSAPPTLRGHAIEARV
                     YAEDPEAGFLPATGRLAMVRWPDAVRVDTGVAAEDEVTDRYDPMLAKVIACGRTRAEA
                     LDRLMAALDETTILGVRTNVRFLRWLLRQEPMRAGEMRTDTVAGMALPHAPVPGDAHW
                     RAAARVLDHAGGLWSGGWRLNAPRVLRLAHDGEERTVSLDASGDPPSAARVGASAFVD
                     VDGQSLEFRIAPPPTIEEAVRHAARASGDEGALLTAPMPGRVIAIRAREGASVHARET
                     VVVIEAMKMEHAVVAATDGTLTRLMVREGQQVQRGDLLAEVTPAGAYHGGDA"
     gene            complement(43170..43952)
                     /locus_tag="VFV59_03375"
     CDS             complement(43170..43952)
                     /locus_tag="VFV59_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5324903.1"
                     /translation="MSGLLRLERDGVHARVVLTRPEVRNAFNAALIDELRTTFEALAT
                     EPPDRLRSVVLAGEGRSFCAGADIDWMRSAIGLSVEDNERDAAAMQAMFAVIDACPVP
                     VIARVQGAALGGGMGLCAVSDVVVATADTTFGFTETRLGIIPAVISTFVLPKIGESHA
                     RALFATGQRFDAERGQRIGLVHEVVPDEVALDERVTALQTELDAAGPEAARAAKALIR
                     DLRGRPADEARAETVRRIARQRTSAEGQEGLTAFVEKRRPRW"
     gene            complement(43949..44761)
                     /locus_tag="VFV59_03380"
     CDS             complement(43949..44761)
                     /locus_tag="VFV59_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEU5324904.1"
                     /translation="MHQPAEDRPTPGSHDRSAPSGSLETSAANLFDAMLAARDAEARG
                     DSAPLHEFYRTLMSGTLLLPVPPGHGEEAKEALAAAVNDDEQVEISVMLAREGEQPVS
                     VVFGSFAALAAWAPANTGNLPLPARIAIGNLAAAGLPAVMDPAGPVPYRFDAEELVAL
                     AEGRIPGTDDPLFAPTARASIRVRLPATDTRDLERSLADALRGGAVDAAYLVETDDGS
                     GSARLLLGLVGEPGTTATVDVPDGTDVVWLEDPLLAQVRAVAEPFHRRGRGR"
     gene            44802..>45394
                     /locus_tag="VFV59_03385"
     CDS             44802..>45394
                     /locus_tag="VFV59_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5324905.1"
                     /translation="MIRGIDHLVVAVTDPDAAAAELESRVGLTCTGGGQHPGAGTVNR
                     LAFLADGSYLELIGVEDPAAARANPVGAASLQMLETNGAGLATFALLDDDLEATLRSR
                     PELGTRTHGSRQRPDGEVVEWWTAAPTEPLSPTSPFLIEHTYAGAEWGPEAMQVRAEQ
                     VHPIGSAVRLVRLDIAVDDPPLVAGELASILDVPVQAV"
BASE COUNT         6316 a        16356 c        16408 g         6314 t
ORIGIN      
        1 cgttcgtgcc gactgggtct cgatgaaccg cgaatcacgc tgcggtcgcc ggccccaagt
       61 gcaatggccg cgacccatcg agacgcgggc gccggatcag cgataccggc acatcagggg
      121 aatcggcgcg atacttgagc catggtggac acgaccaaga ccgtcggcga gcacttcgac
      181 gagtgggcgc ccagctacga cgcccagatc cgcgagatgg tcccgcgcta cgaggagatc
      241 cacgagaccc tgctggcgct tctcgcgctg cggccaccac atcggttcct ggacctcggc
      301 gccggcaccg gctacaccct gcgccgcgtg ctggagagct tcccggagac gaccgcgctg
      361 ggactggacg tgtcagccga gatgctggcc cgcgggcggc agcgcctgga agggctggag
      421 gatcgcgtcg agctgcggca gaccgacctg gtgtcgccgg agatcgacgg cgcgtacgac
      481 gcgatcgtca gcattctcgc cgtccaccac ctgtatgccg atgagaagcg ccacctgttc
      541 agccgcatct gggagcacat cgagcccggc ggcgtcttca tgctggccga ctacttccgc
      601 cccgcgcgca accggctgag tgagctctac cacctccctg ccgagccgga tccgcacgag
      661 gtcgagcacg accaccccga tacggccgcc gatcacctcg cctggctgat cgccgcgggc
      721 ttcgccgcgg tcgacgtcgt atggaagtac gacgacgtcg gcgtgctggt cgcctggcgc
      781 gacgagcgct gagccgatac ggtccgccgc tcgagatcca gcagccagcg cttcgcgtcc
      841 agcccaccgc cgtagccgcc gagcccaccg cccgccgcga tgacacggtg gcacgggatg
      901 atcacggcca accgattcga gccgttcgcg cggcccacgg cccgtgaggc tgccgggaca
      961 ccgacgcggc gcgccagctc gcggtacgag atcgtctcgc cgtacgggat ccgctgcagc
     1021 tcgcgccaca ccagctcctg gaacggcgag ccggggacgt cgagcggcag gtcgaactgg
     1081 cggcgctcgc cgtcgaagta ttcacgcagt tgcgcctcgg cccggtcgag caagggggag
     1141 gcgccgtcga cgaccgtgcc gaagcgacgt cggacgccgg ccacctgcgc gggatacatg
     1201 cgtcgctccg cgaactcggc catgacgacg gcggtgtcgg tcgccccgag caccatggga
     1261 ccgatgggag tgtcgacgga acggacgacg acggtggtgg ccgcgtccgg gtggcagcgc
     1321 aggcaggcgc ggtagcctgc gacacgcgcc gtcagcccgt caggcaggta ggagacgttc
     1381 ttcggcagcg gcttgcgtgc gcggcacgac ggccggcagt agatgccggt cgtgcggacc
     1441 gcggcgacga accggccgtc gtaggcggga tcggagtcgt agaaggcgcg ctcgtactcg
     1501 atgctcatgg ggatggaacg ccgcgacggt cccaggcgtg agacgggccg tgccgacgcc
     1561 ggctcaggcc tggccggttg gctggcgcag gatcaggttg accaggaacg gcgacgagcc
     1621 gaggaccgtg tcaccggagc ggacctcgat ccggtagcga ccgatctcct ccaggcgcat
     1681 gcccttcagg tcggcgacga tgttcgcggt cgtggcggtc ggcgggacgt tgatgcggag
     1741 gcggggcgcc tgctgcagcg tcggcgtgcc ggacggggcg acgacctgga acgagagctc
     1801 gtgctcgccc ggcgtcgcct ccaggatggc gacgatgaag catcggccgt gcgtggccgg
     1861 gagccggttg acgttgatct ggctgaagac gccgaccgcg ctgatcttgc cgtcctgcgc
     1921 ggtcagcgcg tagtcgcaga ggaggagggt gtggagctgc acatcggcca tggctcggcg
     1981 aagcctacac cgatgcgccg tcagcccttg cgctcgacgg gtagccccgc ccgctcccag
     2041 gcgatcatcc cgccgcgcac gttgaccggc cgttcgaagc cgttgcgctg caggaacgcg
     2101 gccgcgtggt ggctccggtt cccgctgcgg cagatcaggt acaccggcgc gttgcgcggg
     2161 agcgtgtcca ggtgcatcgg cagctgctgg agcgggatgt gccgggcctg cggggcgtgg
     2221 ccggcgcgcc actcgtgcgg ctcgcgcaca tcgaccagca ccgcgccgtc ctccggcagc
     2281 gcatcgacga cctgcttgac gtcggcctcc tcgaccttgg gcccgaagag gaagctcatc
     2341 gtgcactgac ctccaccggc tgtcgcaccg gctcctgctc atcggtttcc agggggagac
     2401 cggcggatcg gtacgcgtcc atgccgccga tgacgtgcgc cacatcctcg cggccctcgc
     2461 gcgccaggag gctggcggcg atgctggagc ggtagccgcc ggcacagacg acgaggatcg
     2521 gccccgtcgg tacctcgtcg aggcggttcg gcagctgggc cgccgggatg tgcaacgcgc
     2581 cggggacgtg gccatcgcgc cactcgctcg gctctcggac gtcgagaacc gtgcccggct
     2641 cggccggcac ctgctgtgag cggacctggc ggagcgtctc gacctcgccg tgccacgcat
     2701 cgaagccgcc gacgaggtag cccacgacgc ggtcgaagcc ggcgcgccgc agctgcgcga
     2761 gggccgggtc gagatcctcc ggtcgctcca ggaccagcac gagctcgcgg tcgatggcgg
     2821 ccatggaccc ggtccacgtg ccgaaggtgt cggagaggcc gatcgacagc gaaccgggga
     2881 tgtggccggc ggcgaagagg tcgggatcgc gagcgtcgat gacggacgca ccgtggccga
     2941 cggcgtggga cagctcgtcc gcggtcatgg ctcgcggcgt cgcgagctcg gcgagggatt
     3001 ccgcgccggc ctggttggtg gggcgcatcc ggcgccagta ggccgggacg ctcggctggt
     3061 cggccaggat gaaccgggtg aatgcgtccg cgtccggctg gttcagcgcc gggttgtggc
     3121 gccgctcgaa gccgacggtc gaccagcgct tgccgccgat gcccgagccg cacagcgacc
     3181 cggcgccgtg cgtcgggtac agctcgatcc agtcggggag cggcgagatg cggttctgca
     3241 gcgtgtggaa cagcaccgcg gcggcccggc gcgcctcctc gggcccgccg agcagatcgg
     3301 ggcgtgccgc cgtgccgacc agcagcgcgc cgccggagag gaggatccag tcctcgtcgg
     3361 tgcgcgacag gtcggcgacc aggaaggcca gatggtcggg tgtgtgaccg ggcgtggcga
     3421 cggcgcgcac ccggagcgag ccgacgcgga gctcgtcgcc atccgacatc ggctggtgcg
     3481 gacgcgtcag ctgggccgtc ggggaggcgt ggaccgtcgc gtcggtcgcg cgccgcagct
     3541 cgtccgcgcc cgagacgtag tcgttgtgca cgtgagtctc gaggacgtcg gtgatccgga
     3601 gcccttcgtc ccccgcggcg tggaggtagt cctcgacgtc gcggcgggga tcgaccacga
     3661 acgccacgcc gccactggcg tcgccgatga gatagctcgc gtggccgagg cccttcacga
     3721 agaactgctg gaggtacatg gttcaggttg cctcgatggg gcgtggctgg tgatggcgtg
     3781 tcatgcgggg acagagccta gcaagacttg cgtgtttgcg caatcaaggg aatcagtcag
     3841 gaacagcacc gacgagctcc gcggcgacgt cgaccagcga gacgtgcggc agcgcgggca
     3901 gctcggcgag tggcacccag cgcgcggcat cggtcgagcc ctcgaactcg tctcgcagct
     3961 ccccgccggt gatccggacg cggtagagga tgcggatgcc gtggaagtcc gtcggaagtc
     4021 cgtcctgcgc gctcggaaac ttggccgcca gcgagtcgac cgccaggagc tcggccacct
     4081 cgccgatcag cccggtctcc tcgtcgagct cgcgcagcac cgcgtcccgc gggtcctcgc
     4141 catggtccag gcccccgccg gggagcgtcc acttgccgtc ggactcgacc gtatagccgg
     4201 gcgtgatgcg cgtgagcagc atggcatcgt cgtggacgca gaccgcgtac gccgagacgc
     4261 ggaggtggcg gcggtactcc agcggcgcgg gctcgtccgt cacttcgtgg cgtactgatc
     4321 ggcgccgagg aagtggaggc tgacgtaggg ctcgtcgccg acgacgaccg agtcgtggcc
     4381 ggggccgatc tcgaacaggt cgccggggcc gacctcgatg acgcgtccat cggtcatctc
     4441 gatccggttg cgcccgctga gcaccagccc cacgtgcgcg acctcgcatg cgctctgccc
     4501 cagcgccgca ccgacgtgct cggaccagcg ccagcccggc tcgtactcgg cccgtccgac
     4561 gctcaggccg ccgatctgga ccaggtcgaa tcgtccgagc gggaactccc ggtgctcgtc
     4621 gggatcgtcg aagcggcggc tgatcatggc gggctcgggc atcggtgtct cctccgtcac
     4681 tcggtgtaca ggtcggggtc gatgagccag gcgatctcgc cggcaccggg ctcagggatg
     4741 cccgggaact ccaggcaggc caacccgccg ggccgcatcc ggacgctcga tgcattcgtc
     4801 aggtggcaga tgatggtcga cagatccggc tcgtggccga cgaacaggac gcgccgcgtg
     4861 tcgccggcgt gggtggccag cgcgcgggcc acgtcgcccc agtcggcgcc gggccgcagc
     4921 cggtcgtcca ggatcggcgt ccggccgatc tgctccgccg cgatctccgc cgtccgccgg
     4981 gcacgcagca ccggggacga gatgaccgcg tcgggtcgca ggttgagccg atgccagagc
     5041 ggcccggccg ccagcagggc cgcctcaccg tcagccgtca ggtcgcgacg ctcgtcgtcc
     5101 gagccctgct cggcccggcc gtggcgcagg aagtagagga tcacaccacg accgtggtcg
     5161 cgctgctgtc gtcgttgggg gtggggtcgt cctcgtcgcc gccccgggtc ccggcgtgtc
     5221 ccgccgtgac gccgcgcgcc tggccggtcc gtcggaccag cacgcggccg ccggccgtcg
     5281 cgccgacctt cgggaaggtg atggccagca cgccgttgcc gtagctcacg ttggcgcgcg
     5341 tcgcgtccac ggcgcagggc agctcgacgg tccgctcgta cggtccgtac gcccactcgc
     5401 ggagcaggta gtcgatccgc tcctgtccct cgccgtgctg cgcggcgcgc agcgtcaggt
     5461 gcccgtcatc ggtcacgtcg atggtgatgt cctcgggtgc gaccccgggc atcggtgcca
     5521 ccaccatcag ctcgcggtcg ttgtcgaaca tgttcaccgg gatcgcggtc gcatcggtca
     5581 ttcggtggtc ctcctctcgc gcgcggtgtg cagggatcgt accgagttgc ggaggggagc
     5641 agaatcagcc gaccacacgc agcaggacca accgatgccc gagcatccct tcgtcatccg
     5701 agccggagag ggacaggcgg ggccgccgac ggccggcatg gagcggcgca ccctggtcga
     5761 acgtccggac gcctgggcgg gatgggtccg gaccgatgcc ggcgtggccg gtggctggca
     5821 ccaccacggc gaccacgact cgtacatcta cgtccttcgg gggtcgctga cgattgattt
     5881 cggacccggc ggccgcgaat ccatcgcggc gggggccggt gacttcgtgt tcaaccccgc
     5941 cgggctcgtc catcgcgaga cgacgagtgc ggacggcgac gccgaggtgt tcgtggtccg
     6001 cgtgggggac gggccgcacc tggtgaacgt cgagggtccg gatccgggct gagtcagcga
     6061 gtcggcatgt ggcgcccgtg cgcctggttt ggcgagatgt gccgcctggc gagcaataga
     6121 cggccgatgc gccggcgggc gagcgattcc acgctcaagc cgaccgcgcg agacccgcac
     6181 cggccggata ctgcctcgat atccgcgcat cccgcggatc ggtggtcccg ttccgcgcca
     6241 tgcgcgccag gcgcacattc cgcgacgacc aaccacacga ggccgatcag tccgggaacg
     6301 cctccggtcc gcgctccgag agctcgcgcg cgtgctcggc cccgtcctcc ggcggcagcc
     6361 aggagtaggg gtagcggtcg tcctccagct cggaggcctg ccgcgtgagg tggagcggat
     6421 ggaacgtgtc gatcatcacc gccagctcct ccgtcgcctc cttgccgatc gacgcctcca
     6481 ccgacccggg gtgcgggcca tgggcgatcc cggccggatg gatggtgaag gagctgatgt
     6541 ccacgccgcg gcggctcatg aagttgccgg cgacgtagta gatgacctcg tcggagttga
     6601 tgttcgagtg gttgtacggc gccggaatgg ccagcgggtg gtagtcgaac ttgcggggca
     6661 cgaacgagca gaccacgaag ttccgcggct ggaaggtcag gtggaccggc ggcggctggt
     6721 ggacgcgccc ggtgatcggc tcgaagtcgc cgatgttgaa ggcgaacggc cacaggtgcc
     6781 cgtcccagcc caccaggtcg aaggggtggt gacggtagtg gtaggcggtg acacggccgc
     6841 gcgtcttgac ctcgacgacg tagtccccga tcgacaggtc cggcgccatc tcgtcgggtg
     6901 ggcggatgtc gcgttcgcag tagggcgagt gctccagcag ttgaccgaac tcgttgcggt
     6961 agcgccgcgg cggctcgatg ttcgacgggc actccagcca cagcatgcgc tgcggcacgc
     7021 cggcatccgg acgcagccgg aaggtcgtgc cgatggggat gaccaggtag tcgccggggc
     7081 cgtacgggat cgggccgaag atcgtctcga ggacgccggt gccctcgtgc acgaagtaca
     7141 tctcgtcggc ttcgccgctg cggtagtacc gctccatcgg ctctgagggc agcaccacgc
     7201 cgaaggtgac gtccgcgttg tagaacagcg gcacccggcc ggagacgcca tccccgcgcg
     7261 cctccaggtc cttcgtgttg accagccgat gccgatgcgc gccggacgcc tcgtccggcg
     7321 actgctcgat gaccacgtcg cgcaccttct cgatctggtg cgtctgggtc ggcggggtgt
     7381 ggtggtagag gaggctcgcg cgcccgctga agccctcgac cccgaacagc tcctccgcgt
     7441 acaggctgcc gtcgggcttc cggaactggg tgtggcgctt gtgcgggacc tcgccgcgct
     7501 gcacgtagaa catcgtggtg catgatcgca cgcgacgaat gggggaggag gaggcagggt
     7561 gatcatcgac ccggggacgg agaagggagc gcgcgtggat gcgcgcctgc gctccgagct
     7621 ggtggcgtgg ctggtgacgg tgagcgccga cggcacgccg gtcccgacgc cggtgtggtt
     7681 ctggtgggac ggcgcgacga tcctcgtcta ctcgcagccc gacaggccga agctgcgcca
     7741 catcgccgcg aatccgcggg tcgccctcgc catgcggacc gatgagcacg gcgacgccct
     7801 catcgtcatc acgggacggg ccaccgtgga tgcggcggcg ccgcgggcga atgagctcga
     7861 cggctacctg gtcaagtacg acgcctcgat cgcgcggctc ggcgcgacgc cggccgagtt
     7921 cggcggcgag tactccgttc ccatccgcat cacgccgacg cggctgcgcg cctggtaggg
     7981 gcgccgatca ggcggcggac gcagcgacta gccggtccac ggcgtcgagc atctcggcca
     8041 ggttgaacgg cttggccagc cacgcgtcgg cgcggatgtt ctccgccgcg ccctcgaggt
     8101 cgcgggcggc ggacaggatc atgacccgtg cctgcggggc gagctcgtgg tcatgctggt
     8161 ggcgtcggaa ctcgaaggcg tccatctcgg gcatcatcag gtcgagcacg atcacctccg
     8221 gcacccagct cgtgaggacg gcgagcgcct cgcgaccatg ccctgcgacg cgcacctcgt
     8281 agccctcgtc gagcagcacc tccgcgagcg tctcgcgcag cgtgtcgtcg tcctcgacca
     8341 ccaggatccg ggcgtcggag ctcatcgctg caccccctcg ttgcgcggca gcgtgacccg
     8401 gaagagcgcg ccgtgaccgt ccgagcgcgg ggcgccgatg atggtgccgc cgtgccgagc
     8461 gacgatctgc tgcgagatgt acagcccaag ccccatgccc tggatgtgct ccgcgttctc
     8521 cccgcggccg aagggctcga agatccggtc cacggaggcc tcgtccaggc cgatgccgcg
     8581 gtcgcgcacc tccagcacgg cgcccgcctc ctcctcggcg agcgtcacgt cgaccggcgc
     8641 ttccggcggg gagtacttca tcgcgttgtc gaccaggttg gtgacgacct ggtcgaggcg
     8701 ggcgacgtcg ccggggatcc atagcacgtc cggaccggtg aaccggatgc ggtgcgtctc
     8761 gccctgctcc tcgaaccgcg cgagcacgtc tcggacgagc gccgcgaggt ccgtgggact
     8821 gagctgaagc ggcgcgtgct cggcggcgag gcgcgagacg tccagcagct cgcggacgag
     8881 gtcgcccaga cgatcggccg ccttcgtcat gcgcttcagt ccggtgcgca gccgctcgtc
     8941 atcgagccgg tcggcgtcgc gcgcgcgcac gagcgactcc gccgagagct gcaggctcgc
     9001 gaccggggtc ttcagctcgt gggcggcgat gctcaggaac cggtctcggg cgcgaagcgc
     9061 ctcctcggcg gcggcgcggg ctcgctgctc gacgaggaga gcccgctgct gctcttcagc
     9121 ggcgcgccgc tcggtgaggt cgcgcgtgat cttggcgtac gcgtatgggg tgccctggtc
     9181 gtcgcggagg gcggtgagga tgacgtcggc ccagaagcgc gagccatcgc gccgaacacg
     9241 ccagccctcg tcctcgtagc ggccgtgctc cgccgcgagg cccagcagcc gcatcggccg
     9301 tccggcctcg cgctcctcag ggacgtagaa gaccgagaag tgctgcccga tgatttcgtc
     9361 ggcggaatag cccttgatgc gctccgcacc actgttccag gtcaggacgt ggccgttcgg
     9421 gtcgagcagg aagatcgcgt agtcctgcac cgcgtcgacg agcaggcgga acgcgagctc
     9481 gccacgcagc tgcggaaccc cggcaagctc gtcgggctcg ggtcgtggat cttcctgtgt
     9541 caccgacatc cgccggggca tcctccgctt cctgaggcgg tcacccgacc gccgggcacg
     9601 tgtcagggct cggcgcgggt cagctcggag tgccctcctc gcgggtagac gatgaggagc
     9661 cgggccgggt cgtcgccgat gacctgggca tcatggggga gcgtaccgtc gagcagcacg
     9721 tagtcgcccg ggcccgcttc cacctcggcc tctccctgca agaaccgtac gcggccggag
     9781 aggacgacgt ggtgctcgtc tcccggatgc tggtggaacc cggtgtgcgc gccggcaggc
     9841 atggtcgcct cgaagatcgc cacgtcccgg gcgatgcccc cgtcgacctg gctcagctcg
     9901 ccgtcggtcg acggaacctc gcgcgacggt cgctcggccg cgcggacgac gcgcggcgcc
     9961 acgctctggt cgagcaggaa ccaggcaatg gggacgtcga gcgcctcggc cagcgccgcc
    10021 agcgtctcca gggacggtga cgccttgtcg ttctcgacct ggctcaggta gccgatgttg
    10081 agtcccgtca ccgcggcgac gcgcgccagg gtcaggccgc ggtcgcggcg gcggcgtcgg
    10141 atctgggcgc cgaccagcgg ccggcgacgg gagggcccgg agctcacggg ccgcagtatg
    10201 acggccgccg acgggtattg acaagggcaa agtctgggcc cacaatatgg ctcacaacaa
    10261 atcccgctgg tctcgaacaa cactttcggg ccatcccacg gacccgccac ttgcacagaa
    10321 aggaaccacc aatgaaggtc gccatcatcg gagccggcaa cgtcggtaag gcgctcgcca
    10381 cctccatcag ccgggccgga cacgaggtca ccatcagcgc cagcacgccg gagagcgcgc
    10441 tggaggctgc ggacgccgtc ggcgtcaccg cggccgagag caacgtcgac gcggcgcagc
    10501 aggcgggcgt catcatcctg gccgtcccgt acgtcggtgc cggaccggag gtcgcgcagg
    10561 agattcgcga cgcggtcgcg ggcaagacgg tggtcgatgc caccaatccg ctgaagccgg
    10621 actacagcgg cctcgccacg tcggggacgt cggcggccga ggagttccag aagctgctgc
    10681 cggacgcacg cgtggtcaag gcgttcaaca ccatcttcgc ggccaaccag gcgtcgccat
    10741 cgcccgaggt cgacgcctac gtcgccggcg acgacggcga ggcgaagcgg caggtcatcg
    10801 gcctggctga gtcgctcggc ttcacgccgc tcgacgtcgg gccactgagc gccgcgcgaa
    10861 cgctcgaggg catggcgtac gtcaacatcg gcctcaacgc ggccaacggc tggtcctgga
    10921 cctcggcctg gaagctcgag cgctgacgat ggccgtcacc cgactcaacc acgccgtcct
    10981 cttcgtccgc gatctggacg cggccgcgga cttctaccgc agggccttcg ggttcgtcga
    11041 ggtcgaccgg ccgagcggca tgcgcgcagc gttcctgcgc gcggcggccg gcgacaacca
    11101 ccacgacctc gggctcttcg aggtcggcgg ctcggcggga cggccgtcca gcggcagcac
    11161 cggtctgtac catctggcct gggaggtcga caccatcgag agcctggcgg agatggccgg
    11221 aacgctgcga gagctgggtg ccctgaccgg cgcctcggac cacggggtca gcaagtcgct
    11281 gtacggacgc gatcccgatg gcatcgagtt cgaggtcatg tggcgcgtgc cgcaggaggc
    11341 ctggggccga ttcgccgagc aggccgtggt catgccactc gacctcgacg cggagctgaa
    11401 gcgctggagc gccgcctcgg tgtgacggag cgctccgagg catgaaggag accggcggtc
    11461 gcgggacccg ctatcacggc gagaccgcgg ccgccgcgcc gcgcctggcg gcactccgca
    11521 gcttcctgca gaccgaggcg ggcggcgcgg tcgtgctgct ggccgcgacg gtggtcgcgc
    11581 tggcctgggc caactcgccg tggcgcgacg cgtaccacgc cctgtgggcg accgaggtcg
    11641 gtctgaccat cggcgactgg tccctgcgct acgacctcgg ccactgggtc aacgacggcc
    11701 tgatggtcat cttcttcctg ctcatcgggc tggaggtgcg gcgcgagttc gacatgggcg
    11761 agttccgcga gcgccggcgg gtcgccgtcc cggtcctcgc ggcgctcggc gggatgttgc
    11821 tgccggtcgc gatcttcctc ttcctggcgc cggacggcga cgccgcgcgg ggctgggcga
    11881 tggtcatggc caccgacacg gccttcgcgg tcggggtgct ggccctggtc ggccgccgca
    11941 gctcgttccg gttgcggacc ttcctgctga cgctggtcat cgtggacgac gtggcggccg
    12001 tggtcgtcat cgcgctcttc tacagcacca acgtcgagct cctcgccctg gggatcgccg
    12061 ccgcgctgct ggtcctgatg gccgtgctgc gccgcgtggg cgtgatgcgg agcgacgtgt
    12121 acgtgctcct gtcggtggcc atctggctcg cgaccctgca gtcgggcgtg caccccaccg
    12181 tcgccggcgt ggcgatcggc ctgctcacca gcgcctaccc accgaggcgg acgagcctcg
    12241 aggaggcgac cggcgtgaca cgcctcttcc gccagcgacc gacgccggaa ctggccctgg
    12301 aggcggcgcg ccgaatccgg acgtccctct cccccaacga gcgactgcaa cacggcctcc
    12361 acccggtcag cagcttcgtg gtcgtgccga tcttcgcgct ggcgaacgcc ggggtggatc
    12421 tcggcggcga catcctcggc cgcgtcctcg gctcgccgct cgttctcgcc atcgtgctcg
    12481 gcctggtggc cggcaagtcg gtcggcatcc ccatcggctc gtggctggcc acccgccgct
    12541 ggctcggtgg ccaggcgctc gccgtcggct ggccgtcgct ggtggccgcc tcgtcggtcg
    12601 cgggcatcgg cttcacgatg tcgctcctga tcgccgagct gagctacacc ggcgagacgc
    12661 tggacgaggc caagctgggc atcctcctcg cgtcgagcgt ggcagccgtc ctcagcgtcg
    12721 gcatgttcca cgccgtggcc ctgctgcctc gcgagctgct gtcgcgcgcg gaggccatga
    12781 cctcccgacc gctctcggac ctgaccgatg cggtcgaccc cgtccgagat cacgtgcgcg
    12841 gaccgctcga cgctcccgtc accctggtcg agtacggcga ctacgagtgc ccgcactgcg
    12901 gcagggtcgc gcccgtgatc cgcgagctgc tcgcccgatt cgaggggcgc ctccgattcg
    12961 tctggcggca cctgccgctg accgacgtcc accccaacgc ggcactcgcg gcgcaggccg
    13021 cggaagcggc cggtgcccag gggcggtact gggagatgca cgacgcgctg ttcgaacacc
    13081 aggatgcgct cgacccggtg tcgctgcgcg gccacgccga acggctgggc ctggacgtgg
    13141 ccgcgttcga ggcggacctg cacgacggtc gccatgccgg ccgcgtcgcg caggacgtga
    13201 actccgccga ggaggccggc gtggccggga cgccgagctt cttcatcaac gaggtccgct
    13261 accgcggctc gtacgacctc gactcgctcg accggctggt gcgacgcgtg ctccgcctgg
    13321 tcgagagccg agcgacggcg gccgctgcga cgaccgaaga gcgagccgcc gactagcggg
    13381 cgaggccgtc cagctggtcc gcgtgctcct cgaggtgcgc ggcgatgaag tgctcgacca
    13441 gccgttcgac ggtcatctcg ccgagcgtgg ggtgaacgcc gacccggcgc caggcctcgg
    13501 gcggccagcc gcgcgccgca tcggtcacgc gcgccagcga ctcccgaacg cgcgccagca
    13561 gcgccgccgg gtcggtatgg cggtcgcgct cgatggccgc gatgcgatcc gcatcggcct
    13621 tggtgcgccc gaacgggacc ggcgaatcac tcgcaccgcc gatcacgcgc tcggcctggg
    13681 ccagccagta gtcgggaaac tcggccaggt gcgcccacac ctggcccgcc tcccagcgct
    13741 cctcgccgcc gggatcgggg tcggtcaggc cggcggggat cggcgcatcc gcatgctcga
    13801 cgagccgtcg ttcggcatcc gccatgcgcg ccaggaggtt gtcgacgctc gcgctcatcg
    13861 cccggggcgc tccaccacgg tcgtcgtctg ctcacccagc cgctcgatgg cgagagtcac
    13921 gacgtcaccc ggctgcaggt agcgcttcag cttctggtcg cggacctcga gcaggcagcc
    13981 gaacccgacg gtcccggagc cgatgaggtc gcccggacgc agccgcacgt cggccgacgc
    14041 gcgctcgatc atctgcccga agctgaagtg ggcgctggac cacgtgccac gcgagagctc
    14101 ctcgccgttg acggatgcgg tcatcgtgag ctcgtatccg gtctcgcggc gggcatccgc
    14161 cagctcgtcg ggtgtgacca gccacggacc gatggtggag gcgaaatcct tgcccttggc
    14221 cgggccgagc cgcacggtcg tctcctcgcg ctggaggtcg cgcgccgacc agtcgttgag
    14281 gatggtgtag cccccgatcg cctcctcgcc gcgcgacgcc tcgaggtcgc gaaccggcgt
    14341 gtcgaccagc gcggcgacct cgagctcgta gtccagctcg ctgctgcccc gcggcgccca
    14401 caccggctca cccgggccgc gcatctcgct gatgttgctg aagtagaaga tcgggaggcg
    14461 gtaccacgcc tccggaatct ccatgtcgcg ccgcttccac atcgtcccga cgtgctgctc
    14521 gaaggcgtag aagtcgcgga aggtcggcgg ctccaggatc ggcgggccga aggcgagatc
    14581 ggcggcgtcc atcggctcct ccggctcggc gtcgagcagg tcggccagcg cgtcgacccg
    14641 caggccgcgc gcgaggtgct cgtccagcgt ggtgaccggc tgccggaaga ggacgctgtt
    14701 gtgggcgcgg cgcggatccg cggcgaccag tgagcgacgg gcctcctcga ggtcgtgcca
    14761 gcgcgctccc tcggcatcga gggccgcggc caggcgccac gcggcgccgg ctggggcgtg
    14821 gcgggagcga acgtgagcga tcttcatcgc cgccagtgta gggcggcgag gccgtcagcc
    14881 cggccggagg cgatcccgca gccgtcgcca gcgaccgatg gcgcgccgat gctcggccgc
    14941 ggtgatgggt cggtcggcgt agcgggcgag ggcgtagtcg gcctggagcg ccgccagctc
    15001 cggccccagg ctcgcgcggc gcgcgtgggc ccgcggtgtc tcgttcggct ggcgcgccgc
    15061 cgttggccgc tgctcgcgaa tcgcctccag cgcggcgagg tacgcggtga cggcgtctcg
    15121 cgcaatcacc ggcgccgccg gtcgggccgg ttgcgcgcga ccgacgcgcg gcaggcggaa
    15181 gaggcccggc gggatgcgga tcgatcgctc ctcgtttgca ctgccgcccc cgcggcgcgc
    15241 ccgggcgcgc atccagacgc ggagcaggac cacgcccagc acgatcagca gcccccacac
    15301 cacgatgacg atgacgatgg gcccgcgcag ctgctcgacg gtgaacttgg gcacctcctg
    15361 gaggcgcgcg atctggtcgg gggtcatcgg cggcggcagc tcgaacccga acgagcggat
    15421 gccccgggcc aggatcgagg cgatgagggc ggcgatggcg gcgaacgtga cgaggatgta
    15481 gccgaggatg ccgaggaccg gctcgaggat gccgcgcgcg accgcatcgg cggggaggtc
    15541 gagcagccag gccgcgggca ggccgatacc gaccaccagc gccagcaccg ccagcacggt
    15601 gccgagccat gatcggtcgc ggcgccagtc cacaccggtc tggcggccga tctcctggag
    15661 ccgagccagg ccggcggcgg tgaagcccgc cgtcaggaag gtgaggctgg acacgaaggc
    15721 ggcatcggtg aagacggggc gcagcgccgg gctggagaac tggccgaacg cccacggcgc
    15781 ggccagcgcg gggagaccga cgaggaccag ccgtgtcagt gcccgatcat ccgtgagctc
    15841 ggcgcgcccg acgccgcgca tcccggcgac gagcatcagc cagccgccgg ggtggtggag
    15901 cagcgcgtcg atcgggttcc cggcaagcag caggtcgcgc gcctccgacg accacagcca
    15961 gccgacgatc cccagcccca gcaacagcag cacgaagctg gtcggctcct cgtccggctg
    16021 cacccagcgg cgctgggccg cgagggccgt gatgccggcg gccaccgaga gctccagggt
    16081 gccgaggagc ggagcccgcg cgtccagggt cgtctcgatg gcgacgtaca ccacgcccag
    16141 gaacgctccc tccgcgacga cggcgaggac gggcagcagc cagcccagca ggcggtcagt
    16201 cagcgaggac cagcgcatgc gcatccctcc atccggggtc caggcttccc gtacgggcgc
    16261 gcaggccggc ggcgcgcgcg gtgccccgtg cggcctcccg ctcgggcccg agcgcgacgt
    16321 ggtcgaccgt gtagcccgag ccgcgcagcc gccgcatcgg gtccaggtac ggcgccgggt
    16381 cgcgggcgct gagcaccacc acggagccgc ccggcacgaa gaggcgcggc accgcgtcca
    16441 gcagcgtctc gaacggcagc gacacgatcg gctggacgcg ggcgaggacg tcgtcgatcc
    16501 gtcccagctg tccgggtgcg gccgtcggtg ggacgtacgc ccagcgccgt ccgccggcga
    16561 gctgcgtccc cagcagcagg ccgcaggcgg cgccgtcgcg caggatccgg cgcgcgagcg
    16621 aggagacggc gacgcacagc gcctccacct gctcgtcgtc gaacagcatc agccagtacg
    16681 cgccgggcac ggtctggagg tcgacggcca gcagcacctg gcgctcgcgc accggctcgt
    16741 agcgccggga gaccggcgct cgggttcggg cggtcgcgcg ccagtggatc cggcgcatcg
    16801 ggtcgccgcg ctggtacggg cgcacgccgg cgaagcgcgc ggggtcggtg aacaggctgg
    16861 ccggcacccg gtggctgccc agcggcgcca tcgctcgttc catgctgcga acgggcacgc
    16921 tccgggggcg gaccaggagc tcggccggca gctcccgctc gtcgctccgc gtcccgcggt
    16981 cgaacaggtc gctcaccgtc agccgcaccg gaccgagcac gaagacgccg cggcgacgcg
    17041 cgtcgatggt caggcgccgc acgacgcgct cgaaccacag cagcgaccag gcgttgcgca
    17101 gcgtgccgcg cccgggccgc tcgctgggcc cgagcgtgtg gcccgcgacg cgaagcgtat
    17161 cggtcacgtg gtcgtcggcg gtcagcaggg gcaccggcag cagcttgcgg ttccagacct
    17221 ccagctccat cggcacctgg tcgccccata ccgctcggtc ggtcgcgagt cggcggtcga
    17281 aggcgatgga ttcgaggccg aagcgcgacc acagcgtgat cagccagcgc gagaggagcg
    17341 tcaaaccgcc aaccagggcg agccccggag caccgatcgc cgcgccgatg acgatcagcc
    17401 cgaggccgaa cagggtccag ccggccgcca tgcgacggcc tcagcccgcc tcggcgccga
    17461 cgccgacggg cacgctttcc aggatctcgc gcacggcggc ggctgcggtc gcgccgcgca
    17521 gctcccggtc gaggtcgacc agcagccgat gggtgagcac cggctcgacc agcgccgtca
    17581 cgtcgtcggg gaggacgtag tcgcgtccct ccaggaaggc ggccgcctgg gccgcccgat
    17641 acagcgccac cgacgcgcgc gggctggcac ccagccgcag gtcggcatgg tcgcgcgtgg
    17701 ctcgcaccag gcgcacgacg tagcgctcga cctcgtcgct gacgcggatg ctgcgaacgg
    17761 tggcgcgcag cgtggggagc tccgcgacgt cggccaccgg accgatcgcc tccagtggct
    17821 cggcggcctc ccggtatcgg cgcgcgatgg cgcgctcctg gtcctcctcg gggtagccga
    17881 ccgcgatgcg caccaggaat cggtcgagct gcgcctccgg cagcgcgaag gtcccctcca
    17941 gctcgaccgg gttctgtgtc gccagcacca ggaagggggc aggcagcggc cgcgtctcgc
    18001 cgtcgacgct gacctgcccc tcctgcatgg cctcgagcag cgccgactgg gtgcgcgggg
    18061 tggcgcggtt gatctcgtcg accagcagga cgttggtgaa gatcggcccc gggatgaagc
    18121 ggaacgcgcc gtcggccagg acgctgctgc ccgtgacgtc ggtcggcagc aggtccggcg
    18181 tgccctgcac gcggccgaag ctcaacccca gggagcgcgc gaaggcccgg gccaggaggg
    18241 tcttgcccac gccggggacg tcctccagca gcgcgtggcc ctccgccagg agcgtgaccg
    18301 tcagcagccg cagcggctcg tcggccccga ccaccgcgcg ctcgaccgag gtccgcagcg
    18361 ccgcccagag gcgggctccg gcgtgtggat cgctcatcgt cggaggatac gtccgcgaag
    18421 ggcctgcacg ttcaacgcgg caaccgtatc gttcgctgcg ccgatgccac cgcccacccc
    18481 gctgatcttc tccgaggcgc accgcgccca cgacggcctg gtcgagctgt tcgccggtcg
    18541 cgagatcccg tgctgggagt cgcccgcccg tgccgagacc atcctcgccg cccttctcga
    18601 gaccggcgat tacgccctgg agccgccgga ggagcacggt cccgatgcca ttgcggcggt
    18661 ccacgagctc gagctgatcg acctggtcga gagcgcctgg accgatgcgg tcgccgacgg
    18721 ccacgacccg gcgcgcagcc tccttcccga taccttcctg ctggccgcgt accgcggctc
    18781 gatgacgctg gagcagctgc cggcccagcg gcacctgcgc ctgggggcct actgcttcga
    18841 caccgcgacc cccatcgtcg cggggacggc ggcggccgcc cgggacgcgg tggacgtggc
    18901 cctgaccgcg gtcgatcggg tgctcgaggg cggggcgcgg ctggcctacg gcctgtgccg
    18961 cccgccgggc catcacgccg gccggaacct catcggcggc tactgcttct tcaacaacgc
    19021 cgccatcgcg gcccagtcga tgcgcgaccg tggcgcggag cgcgtggcga tcctggacgt
    19081 ggacttccac cacggcaacg gcacgcagca gctgttctgg gagcgcggcg acgtcctgta
    19141 cgtcagcctc cacggcgatc cgcgggtcag ctacccgtac tactcgggct tcgcgacgga
    19201 gcgcggcgcg ggcgacggcg agggcgcgac ccgcaacttt ccgctcccgc ccggtaccga
    19261 tggccacgcg tacctcgcca ccctcgacga ggcgctcgcc accatccggg ccttcgacgc
    19321 ggatgcgccg ctggtcgtgt cgctgggctt cgacacctac gagaccgacc cgatcagctc
    19381 gttcgcgctg cggaccgacg actacgcccg gatgggtgcg cgcatcgcgg acctcgggat
    19441 gccggcggtg gtgctccagg agggcggcta cgcggtcgac gccatcggtc gcaacgcggt
    19501 cgcgttcctc ggcgcgctgc gggacgggtg accggctcga ccccacgccc ggcgctgtgg
    19561 ccggcgtacc tggcgacctt caccttcatg ctcggcggct gggccacgtc catcgccgtg
    19621 ccgctgaacg tcgtgctgct gggcggctcg ctggccgagg ccggggtgct ggccgccatc
    19681 cggttcgggc tgcaggcgtt cctccagctc cccttcggcg cggtgatcga tgcctggggg
    19741 acgcgccgcg tcctgttcat cgccacggcc ggcaacgcgc tggtgaacgt ggtcccggtg
    19801 ctggccgtcg tgaccggcag catcctgccg ctgtacgcgt gggcggtgct gtcgggcgtc
    19861 agcgcgtcgc tcttcctgcc gtcgaccagc gcctacatcg ctgccagcgc cgagccgagg
    19921 acgcgcggca gcgccttcgg gtggctgacc ctgttcacgc ataccggcgt cgcgtcgggc
    19981 ccggccgtcg gcggggtgct gtgggacctc gccggtccgg ctccgaccta cgtcgtggcc
    20041 ggtctcctgg gcgccatcgc ggtggtcgcg ccgttcttcg tgcccgcggt cgtcccgcag
    20101 cgcgtgcggc tcacgcgtct ccccggcatg gtggccgagg tcgcgcggca gcgcgcgatc
    20161 gtcggctcct gggtcgcggc gctggccatc gggttgccgt ggggcgcggt ctcgggcctg
    20221 ttcccgctgt tcggcaccgg catcggcctc acggccggga cggtcggcct gctcatggcc
    20281 agccagtcgg tcgcgaacgg cgcgtcccgg gtgccgttgg ggcggacgat cgatcgcctg
    20341 ccgatcccgc ccatcgtggc ggcgctgacg gcggcgggct acggcgtcgt catcggtctc
    20401 ctgggcctcc agggcaacgt cgtgctcatc ctcgtggtgc tcgtggccgg aatcgtcatg
    20461 ctcgccttca cgctgatgct ggtgcaggtg accatcagcg aggtcgcccg accggaggtg
    20521 cgggccacgg gcctgggcgg gtacggcacc gcgctgtcgg cagggctcgg actcggcccg
    20581 gcgctcggtg gcgccatcgc cgacgcggcg ggcttcggct gggccttcgc gaccatcggc
    20641 gttgccgggg tggcggtcgc cgcggtggcc gcgctggtgc tcataggcac gcgccggcgc
    20701 ccggccgtgg tcgtcttcga cgcttgaccg atactgtgcc cagcacgcag cggagcaacg
    20761 ttcggtgaag cgcatcggct tcctctcctt cggtcactgg tcggactcgc cctactccca
    20821 ggtgcgctcg ggctcggacg ccctcatcca gtcgatcgag ctggccgtcg cggccgagga
    20881 gctgggcgcg gacggcgcgt acttccgggt ccaccacttc gcgcgccagc tggcatcgcc
    20941 gttcccgctg ctggcggcgg tcggcgcccg tacgagccgg atcgagatcg gaaccggcgt
    21001 catcgacatg cgctacgaaa acccgttcta catggtcgag gacgccggcg cggccgacct
    21061 catcgccggc ggacggctcc agctcggcat cagccgcggc tcaccggagc aggtgatcga
    21121 tggctaccgg cacttcgggt acgtcccggg cgagggcgag accgatgccg acatggcgcg
    21181 acgccacgcg gagctcttcc tgaagctgct ggacggcgag ggcttcgccc aacccaaccc
    21241 gcggccgatg ttcccgaatc cgcccggcct gctgcgcctg gagccgcacg cgccggggct
    21301 gcgcgaccgg atctggtggg gcgccggctc gacctccacg gcggtctgga ccgcgcagct
    21361 cggcatgaac ctgatgagct cgaccctggt cttcgacgag agcggcgagc cgctgcacgt
    21421 gcagcagcgc cggcagatcg acgccttccg tgaggcctgg aaggaggccg gccaccagcg
    21481 cgagccgcgc gtatcggtca gccgcagcat cttcgcgatc gtcgacgacc gggatcgcgc
    21541 ctacttcggt cggggccagc aggagcacga ccagatcggg cagatcgatg agaagacacg
    21601 ggccgtcttc gggcgctcgt acgccgccga gccgcaggcg ctggtcgacc agctgcgcga
    21661 ggacacggcc atccaggcgg ccgatacgct cctgctcacg gtgccgaacc agctgggcgt
    21721 cgactacaac gcccacgcca tcgagtcgat cctgaagttc gtggcccccg agctgggctg
    21781 gcgctgacgc ctccgctcgg tcgccggctc agcgccaggc gatcgagacg acctccaccg
    21841 gctgctcgat gcccttcagc tcgatcgttc ggcgctcggc ctccggcacc ggccgccccg
    21901 ccgccgcgac ggtaccggcg ctggccagga tctcgccccc ggccgcagcc gcgccgatcc
    21961 gggcggcctg gttcaccccc acgccgatgt agtccagccc gctgcgcgtc gcctcggcct
    22021 ggtgcacgcc gatgcggacc tccggcgcga agccgtgggt ggcgcgctgc tcgaccagcc
    22081 ggcgctggat ctccacggcc gcgtcgatgg cggcgcgcgg atcctcgaag gccaggaaga
    22141 agccgtcgcc ggtggccttg atctcggtgc cgccatgcgc cgcgacgacc gcgcgcaggg
    22201 tctcgtcgtg gcgtcgaagg aggcgcgtcc acgcctcgtc gcccaggagc tcggccaggc
    22261 gggtcgagtc cacgatgtcc gtgaagacga aggcgcgcac gtcgcggcgc gcggacgatg
    22321 aggtggcgcc tgcgccgcgc gcctccgccg gcagtgcggc gatcagcgcc tcggtgcggc
    22381 gcagcccggg agtggccccc atctcctcga acgtcgctcg ggcgctctcg gcctcggcca
    22441 gggcgtcgtc gggctgtccg gcggccgcgt acgcctcggc cagccgaagc cgggcgcgcg
    22501 ccgcctcgta cggcgagccg aactcgagcc aggcctgcac cgcggagcgc agcgtgcggg
    22561 tcccgttcgc cgtcgcgccc tccgccacct cgacggcgcc ggtggccgcc agcgcggtgg
    22621 cccgcagctg gagcgtcccg tagcggccgg cggtggcggt catctcgtcc gccgcggcgc
    22681 gggccgtccg cacgtccctg gcctcgaggg cgatttccac ctgcgccgcg agcaggggca
    22741 tgcggtacac gtcgctcccc ggtggacctc gccacgccgg cgttcgggtc ggctcgtcga
    22801 gggcgcggcg gatgccggcg gccgccgcgg cggcgttgcc ctgcgccaag cgcaggagcg
    22861 agagggccgg ctccggatcg gtgcccagcg cgtggatccg ctgcagcgcc tcctcggctg
    22921 ccggcaggtc accgcgggcg agccggatct cggcgatctg gtacagcgcg ccgccgacgg
    22981 cggtggggat gtagcccatc atctcgtcgg acgcgcgtcg ggcctcggcc tcggcctcca
    23041 gccacgaccc gcgcagccgc ttgatctcgg ctcgaaagag gcggcacatg ccggggtagc
    23101 cgctgatccc ctcgcggcgg caccagcggt cctgcgcctc ggtccactcg accgcgcgcc
    23161 cccactcgcc gatcgtcgcg caggactcga tggtggtgca gcagatgccc ccggcggtcg
    23221 ccgggtcgag ctcgccaccc accgccgcca ccgtggcctc gtccaccagg gccagccctt
    23281 cctcgaggcg gccgagcgcg accagcgccg cgccccgctc gctcatggcc agcgcctcga
    23341 ggttgcggtc accgaatcgg gcggcgatct cctgggccct ggtggcctcc tccagcgtgc
    23401 gctccggcgc tccggccagc gcgtcgtgga agccgcgcgt ggccgcgacc cagccgtgcc
    23461 ccggattctc tgggacgccc tccaggagcc ggtccgcgcg gctgagccag gcgctcgcga
    23521 ccgagagcgc gttgcggaac aggtggtcac gggccagcca gacggcgacg agcaccgccg
    23581 tctcggtctc gccgcccttg atggccgcgg cgtacgcgcg ctcccggacc tcgatcgcgg
    23641 tcggcagcat gccgagccac cacgccgcct ccgccagcag ctccagctcc tgcgcgtcca
    23701 ggctgccggc gcggtcggcc tcggccagca ggtcgtacgc ctcgcgccag gcgtgtcggt
    23761 cgagggcgga ccgtgcggat tcggcgaggc tcgaggcggc catcggtcgc cattctgcca
    23821 tgcgccgagc tcctgcggtc cgaaaggtat atcggtatat acgccgcggt ggagcggaac
    23881 ctcacgcgcc atactcgtcg cgtgaggcag cgacgagagc cgagggcggg cgccccgcgc
    23941 gagacgagct gggagcgcgt ggcgacctgg tacgacgggt gggtgggcga cgcgggctcc
    24001 acctaccacc gcgagctcgc catcccggcc accctggacc tgctgcagcc gcagccgggt
    24061 gagcacatcc tcgatgtcgg cggtggccag ggcgtgctgg cgccctacct cgtcgaggcc
    24121 ggcgctcggg tgaccgtggt ggacgcatcg gcgaagctga tcgccgccgc caagcgacgg
    24181 cacgcgaggc tgcgcgacgc caggttcctg gtcggggatg ctcggcgact gccggcggtg
    24241 agcggcctgg agccacacgc gtgcgacgcc gcgacgttcc ttctttccat ccaggacatg
    24301 gacccgctcg acgacgtcat gcgcggcctc gactgggcgc tgcgaccggc cgcccgcgtg
    24361 gtgctgctga tgacgcaccc ggcgttccgg caggcgcggc actccggctg gggatacgac
    24421 gagggccgga agctgaccta ccggcgcatc gacggctacc tgaccgagct ggcggtgccg
    24481 atgaagtcgc tgggtggcgg cctcccgacc cgcagcttcc accgaccgat cagccgctac
    24541 gtcaacgccc tggccgattt cggatttgcc acggatgcga tgctcgagct gccggacctg
    24601 ccgccggatc gccgtccggg caaggccgcc cgcggcgacg cgcgcgccaa cgccgagatc
    24661 ccgatcttcc tgggcctgcg cgccgtccgc cgctagcgcg cgaagcgcat cggtccgttt
    24721 cgggcgagaa tgcgcacccg ctgaatcgcc acgcacagga tcaaccgatg ctgaccacct
    24781 ccccgcgccg gccgcctgcc gccgctgccg ccgtccttct cgccgtcctg ctcctcttcg
    24841 gggcgctgac cttcgtcgcc ggcctcgcgg tcggtgggtc gggagagggt gcggccgccg
    24901 cttcgccctc gtccagcggt gccgtctcct cggatgccgg cgtcccatcg tcctcgccga
    24961 agcccgacgt cgcggtcgag acggtcacct gcgccgagcc gaccgaggcg tgggccgccc
    25021 tgtgcgacgc gtacggccag atccgggagg agtacgtcga cgaggagcag atcaccgacg
    25081 aggagctgat cgacggggcc atccgcggca tggtcgagta cggcctgggc gacccgtact
    25141 ccggctacct gacccccggc cagtacgggg aggcgctgca ggacctgtcc ggcgagttca
    25201 gtggcatcgg tgccgaggtc gggatggaga acacggccaa ccccgacgac ctggccgcct
    25261 gcaccgtcat caccgagacg tgcgccgtgg tgatcgtcgc gccgatcgac ggatcgcccg
    25321 cggaggccgc gggcctgcag ccgggggatc ggatcctgga ggtcgacggc cagacgacgg
    25381 tcggggagac gctgagctca ctcgtgtacg tggtgcgcgg cgaggagggc accgacgtga
    25441 cgctgaccat ccagcgcggc gaggacacgt tcgacgtcac gatcacccgc ggggtcatcg
    25501 acctgcacga ggtcagctcc gagctgctcg acggtggcgt cgggtacgtg cggctcagct
    25561 cgttcacgga ccgctcgacc gacctggtgc gcgaggcgat gcagtcgttg ctggacgacg
    25621 gcgccacctc gttcgtcctc gatctgcgcg ggaacccggg cgggtacatc gtggcagccc
    25681 agggcgtcgc gtcccagttc gtccccgagg gcgagctgct cttcaccgtc gagtccgacg
    25741 gcaacgtccg cgagtggcgg gcggaatccg gcgtggcgca gtcctccgac atcccggtgg
    25801 tcgtgctcgt cgacgcagga tcggcctcgg cctccgagat cgtgtcggcc gcgctgcagg
    25861 agacgggccg cgccaccatc gtcggcgagc cgaccttcgg caagaacacg gtccagatct
    25921 ggaacgacct gcccaatggc ggcggcctgc gcatcaccac cgaccgatgg ttcacgccgg
    25981 accacaactc ggtggcgccg gacggcgtgc agcctgacct cgtggtcgaa ggtccgcccg
    26041 atgcgacctc ggccgccgat ccgcagctgg accgcgcgct ggagatcctg cgcggcggct
    26101 gagtcggcgg tctctcgtgg ggcggccgat gcatcagccg ttctgcacgg gagggccgtt
    26161 cggcgtgcgg ttcggccgct gcatcggtca gccgggagcg tgcaccgcac cgtccggtgc
    26221 atgtcgtgcg cgccagtgac cgacgcgccg gtcatccggc accggaaagg ccgctcacgc
    26281 gtcacctgac cgatccagcg agcgcgacgg gagctaaccc ccggacagca ggcgcaggta
    26341 caggtccggg tccacgtttc cgccactgat gacgatcacg cgggcgacgt cgtcatcggt
    26401 tcggggcagc cggtcgttca ggtgcgccgc catcgaggcg gcgccggagg gctcgaccac
    26461 gaggcgcgcg cggcgcgcca gctggcggac cgcatcggcg atctcgtctt cgctcacggt
    26521 gacgatcccg tccaggaagc ggcgcaggtg ctcgaacggg agccgcccca gcgagttggt
    26581 gcgcaggccg tcggccatgg tccggccggt ctgctcggca gtccacgcca ccaggcggcc
    26641 ggattcgagg gactccatgg cgtcggcggc gaactcaggc tccacgccga ccacccgggc
    26701 ggacggcgcc agcgccttga cggcggtcgc cacgcctgcg gacagaccgc cgccggagac
    26761 aggcacgagc acgctcgtca cctcgggcag atcctcgacg atctccaggc cgcacgttcc
    26821 ctgcccggcg atgatcgccg cgtcgtcgta cggctcgacc atcacgagcc cgcgctcctc
    26881 gaccagctgg agcgccaggg cgtgccgctc cgcggaatcc atgccggtga actggatctc
    26941 ggcgccgtcg cgccggacgc cctcgatctt caccgcgggc gcctcgcggg gcatgcagat
    27001 caccgcgtgc gtcccgagca gccgcgcggc ccgggcgacc gcctgcgcat ggttgccgga
    27061 cgagtaggtc acgacaccgc ggccccgctc ctcgtccgac agggaggcga tcttgttgta
    27121 cgcgccgcgc atcttgaagg cgccgatcgg ctgcagggac tccggcttca gccaggtcgt
    27181 gtcgtcccag cgcagcagcg gcgtacgggt ggccacacca cggatgcgct cggcggcctc
    27241 ccggatctcc tcgacgctga cgatgtgcgg ctcgcccatc agtacgccct ggcccatgtg
    27301 gccagctcgg ctcccggcgc accgcagtgc acgcaggcgg cgccaggatg gcgtggctcc
    27361 agcggcagga agcggatcgt cgccttggtc ttggcgctga cctccgcctc gcacgccgcg
    27421 tcgccgcacc aggcgccgac ccagaagcca ccctgctcct cgacgcccgc ggcgaagtcg
    27481 ccgaacgacg tgatctcgtg ggtgttcgcc tcgcggaagg ccagcgcatc ggcgaagagc
    27541 tcgcgctgca ccgatgccag ccgctcggcc atgtcggccg cgaggacgcc caggccgacc
    27601 tcctctttcg cgccggagac tcgggaagcg acgaccgccc ggttcgcggc cacgtcccgc
    27661 ggcccgagct cgacgcgcag gggcaccccc ttgagctccc actcggcgaa cttgaacccg
    27721 ggacggtgct ggtcccgatc gtcgagccgg acgcgcaggc cgcggccggc gaggtcggcg
    27781 cgcagcgcat cggcgacgga gaggacggcc gaacgctcct ggtcggagcg gtagatgggc
    27841 acgatgacga cctggaccgg ggcgaccgag ggtggcagcc gaagcccggc gtcatccccg
    27901 ttggccatga tcgtgccgcc gaccatgcgc gtgctgaagc cccaggacgt ggcccaggcg
    27961 tgctcctgca cgccatcggc gttgaggaag gtcacgtcgt aggccttggc gaagttctgc
    28021 ccgaggaagt gcgaggtgcc gcactggagc gccttcgcat cgcgcatcag tccctcgatg
    28081 cagtaggtct cgaccgcgcc ggggaatcgc tccccgggcg acttgcgacc cttgatcacc
    28141 gggatgccga gcacgtcctc gctgacctcg cggtagacct ccagcatgcg gagggcctcc
    28201 tcggtcgcct ccgcggccga cgcgtgagcg gtgtggccct cctgccacag gaactcgctg
    28261 gtccgcagga acagccgcgt gcgcatctcc cagcgcacca cgttgcccca ctggttgtag
    28321 agcaggggca ggtcgcggta gctctggatc cagcgcttgt aggtcgacca gatgatcgac
    28381 tccgacgtcg gtcggatggc cagcggctcc tccagctcct gcccgccccc acgcgtgacc
    28441 acggccacct ggggagcgaa gccctccacg tggtcggcct cgcgatcgag caggcccatc
    28501 gggatgagga gcgggaagta gacgttcttg tggccggtgg ctttgaagcg gtcgtcgagg
    28561 gcgcgctggg tgagctccca gacggcgtag ccgtggggct tgatgatcat cgaccccttc
    28621 gccggcccgt gctcggccag ctccgcctgc cggacgacct cggcatacca gccggggaag
    28681 tcgtccgcct gcgagggaag ccggtctgcg cgcgttgctc tggagcccat ggacgcccga
    28741 tgctagccca tgcgcgacgg cgccctgccc cgccgtctac ggcgcagtga tgcggtacac
    28801 gaagcccgcc ttgtcatcgc tgacgtacag gccgccgtcg gcgccgaccg ccaggccgac
    28861 cgggcggccc agcgcgtcct ccgccccatc gggcaggaac cccgtcgcga gtggcgcgac
    28921 atctccggcc ggaccgtcat cccacggcag ccaccacagc gcgtacccga ccttgaccga
    28981 tgagttccac gagccgtgca gcgcgatgac caggtggtcc tgccactcga ccagcccgag
    29041 gggcgcgacg tgggcgggca gggtcagcgc cggctggagg accccgtcgc aggcggtcac
    29101 gtcggtcccg aactcggggt cgcgcagggt gccggcgtgg cagcgcggcc agccagcatc
    29161 ggtgccttcg atcaggcggt acagcgtctc gggcggccgg tcgtcaccca acaggtcgcg
    29221 gcccatgttc gttgcccatg gctgtccggt cgccggatcc acccacagtc cgaccgcatt
    29281 gcggaggccg atgacgaacg gccgcagctg gccgtcctcc aggacctgca cgctggcgcg
    29341 acgctcgtcc tcctcgtcgc acacgttgca ggaggagccg atcgagacca ggacgcgacc
    29401 gtcaggcagt accgcgaggg atttcgtgga gtggaagccg ccggtcggca ggccgtccac
    29461 cagtggtcga cggtccgcct cgcgcgattc gtcgagcgtg acctcccaga tggtcgactc
    29521 gccggcgacg agcagccggc cgtcgtccgt gaaggcgagg tcctgcgcgc ggccatagcc
    29581 catgccgacc tccacgacct cgtcggcgcg gccgtcgccg ttcgcgtccg gcagcgccac
    29641 cacccggtca gccccgcgct cggcgacgaa gagcgtgccg tcgggcccga cctcgatgaa
    29701 gcgagggttg tcgaggccct cggcgaagac cgacgcgctg aagccctcgg gtagggtgag
    29761 ctcgacgcgg ccgccgctcc ccgcatccag gttcacgccg aagaacgggg ccagctcggc
    29821 ccggaaggtc cagaccagga tgccgatcac cgcgatcgcc acgatcgcga agagcaggag
    29881 gttgcggacg cgcggccaat ggccgaaccg atgctacgca gccgcgcctc gggtccgctc
    29941 cgtcagaggt tgccccggcg ggcctgctcg atctcgatcg cctcgaagag cgccttgaag
    30001 ttgccgacgc cgaagccacg cgacccgtgg cgctcgatga tctcgaagaa gaacgtgggc
    30061 cgatcctgca cggggcgcgt gaagatctgg agcaggtagc cctcctcgtc gcgatcggcc
    30121 tcgatgccga gctcctcgag cacgtccatc ggcacaccga catcgccgac gcggtcgggc
    30181 agctgctcgt agtaggcgtg gggcatccca aggaactcga cgccgcgctc gcgcagcgcg
    30241 cgcacggtcc ccacgatgtc ctcggtccgg agggcgatgt gctgcgtgcc ggcgtccagg
    30301 tagtagtcga ggaactcctg gatctggctc ttcttcttgc cggtcgccgg ctcgttgatc
    30361 gggaacttga tccggccgtt cccgttctgc atgaccttgc tcatcagcgc cgagtactcg
    30421 gtgtggatca ccttgtcgtc gtaatggatg agctgcgcga accccaggac gtccttgtag
    30481 aagtcaacga accgattcat gtcgcccagc tcgacgttgc cgacgcagtg gtcgacctcg
    30541 agcaggctct gcccggtcgc ggcgggcgga ggcgccttca ccctgtggta gcccggcgcg
    30601 aaggtgccgt ggtagtcgga ccgatcgacg aacgagtgga tcacctcgcc gtaggtgtgg
    30661 atggccgact tgcgcagcct gcccttctgg ccctcgagct cggtgggctc gagcgccgag
    30721 accgcgccgc gcttcgtcgt ctcctcccac gccgacgtga cgtcgtcgac ggcgaaggcg
    30781 atgtccttca ctccgtcacc gtgcttcgcc acgtgctccg cgatctcgcc atcgggggac
    30841 agcggcgcgg tgaacacgaa gcggatgtcg ttctggacca tgacgtagct ggaccgatcc
    30901 cggacgcccg tctccagacc cgcgtaggcc accggggtga agccccacag cgtccggtag
    30961 tagtgcgcgg cctgccgggc gttgccgacc cagaactcga tgtggtcgat gcccttcagc
    31021 ggcaggaaat cctgcgcctc ggcggcagcg ttcgggttga cgatcgcgcc ggcctggttg
    31081 gcggctccgc gcgtgagcgt atcggtcatg ggtcgaggat accgcaggac cgatgccgcc
    31141 tcagaggaag cgcaggagcg gcatgagcgt gaacacggcg aggatgccga tcagctccag
    31201 gcggaacgcc agccggagcc gagtcacgag tggcgcgcgc gcctcactgc catccccacg
    31261 cggaccagag gtccagtacc ggtcgtcgtc cagcagtgcg cgcgccgtcc ggctcgtcac
    31321 ggtaccgccg actgcgaaca ccatgacggt gatggcgatg gccgccgacc aggcgatccc
    31381 gtacggcgtg gtcagcgagt cgaccgagtc gatcggcccg tacgccgtgc cacggacgat
    31441 gccgaggacc gcagcgagca acgctgcgcc gatcatcatc cgctccggcc cgaagatgac
    31501 gcgacgcgcc gatgcccgtt gctcgacggc tggcagcgcc aacagcgcgg ggaggacgac
    31561 gacgttcatg aacaccgaac caccgagcca gacggcgccc gcgaggatgt gaagcgtttg
    31621 gaccaggagg aggacggggt ccacctggct cacctcccgc cgaaggtccc cgcgttgagc
    31681 gcgacgccga tcatgcggtc gatggcgcgg tcgagctcct ccagcggcag gtacgactcc
    31741 tcggtcgtga cctcctcgga cagcacgtcg ctgaccgtca gcgcgcacgc ggcctgcgcc
    31801 ccggagcgcg cggccaggaa gaacagcgcc gaggcctcca tctcgaaggc cagcacgcca
    31861 cgctcccgcc agcgcctggc gtagtcgtcg tccgggttgt agaagacatc gaccgatgcg
    31921 acgaggcccg ttcgggcagg cacgccgtcg gtgcccgccg cgtcgaccag tgcgcgggtg
    31981 agctcgaagt cggccaccgg cgcgtaggcc tcaccgtgca ggtacgttcg cgtggcgccg
    32041 tcgacggggc aggcgccggt ggcgatgacc agatcccccg tcttcagtcc ggtggcgatc
    32101 cctccgcagg tgccgacgcg gatcaggcgc cgcgcgccca ggcgcaggag ctcctccacg
    32161 acgatcgaga tgctgggcgt gcccatgccg ctggtctgca cgctgaccgg cgtgccgcgg
    32221 tacgtcccgg tgaagcccag caggccgcgg tgatcgttca cctggcgcgc cgagccgtcg
    32281 tcgaagcgct ccgagatgcg gcgtgcgcgg ttcggatcgc cgggcaggag gaccagcggc
    32341 gcgtagtcgc cctcctcggc gcggaggtgg atctgtggca tggcgtgatc ctactcgccg
    32401 aggcccacgg gcgcggtggc gtgtgacttg catccggcgc cctcgcatgg ggaaggcgag
    32461 caagcgatcg cgcgaccgcg aggacgtcgg gcagacggcc cggcagctgc tgcgtgccgt
    32521 gggtggcggg ctgatcatcg gcctgccgct gctcttcacc atggagatgt ggttccactc
    32581 cttcctgctc agctcgtgga agctcgtcgt gctgctgctg atcgccttcc tggtggtcat
    32641 cggctacagc gcgctgagcg gcttccggca cgagcgaacc tggggcgagc tcctggtcga
    32701 ctcggtgcac accatgggca tcgcggcggt ggtgtcggcg ctggcgctgc tgctgctggg
    32761 gcgcatcgac cccgacacgg gcctgcgcga cgcggtcggg aagatcgcgc tgcaggtgat
    32821 cccggtcgcc ttcggggtct cgctcgcggg cgcgcaactg gccggcgggg acggcgagga
    32881 cgaggaggac caggggagca gcgcgaccga cggcggcggg cagctgggcg ccttcgctcg
    32941 acttttcgtg ggtgccgggg ctgcgctgct gtttgccctg aacatcgcgc cgacggagga
    33001 gccgatgctc ctcggcgtcg aggcggagtg gtggctgctg ctcctggtgg tggccgccag
    33061 catcgccatg acgctggcga tcgtgttcta cgcggacttc cgcggcggcc gctccccgga
    33121 gggcggtgac agcatcctcg accatccgtt gaccgaaacg gtcgcggcgt acgcggtcag
    33181 cctggcggtc agcctgctgc tgctgtggtc gttcggccgc acggacggcg cgtcgtggtc
    33241 ggccatcgtg gggcagacgg tgatgctcgg cttcgtggcc gccttcggcg ccgccgcggg
    33301 ccgcctgctc gtcggcagcg gctccgccag cagcgcggga gccacctgaa ccgatgcgcc
    33361 agaactggct cgagtgggtc gcactggcca tcagcgtcgt cgccatcgtg accgtcgtcg
    33421 gcttcctggt ggtcgacggc atccgcgacg agggccgacc gccgatgccg gtggcggagg
    33481 tgcgaatggc cgaggcgtac caggcggaga ccggctggct ggttccggcg accatccgga
    33541 acgacgggga ccaggcgggt gaggccgtgc agctggtggc tcgcgcttcg gtcggtggat
    33601 cggaggagga ggccgagctg accttggact accttccgtc cggatccgag ctggacgtcc
    33661 tgttcggctt ctccgggcgg ccggacggcg aggtgaccgt cacggtccgt gggctccggc
    33721 taccctgagc gcatgacccg ctgggccgac gacctcgcct tcgccaccga tctcgcgcgc
    33781 cgcgccgggg cggtgctgac cgatcactac gagcggctcg agcgcatcga ctacaagagc
    33841 aagcgcgatg tggtcacgaa cgccgactac gcgtcggagc acctggtgat cgacgccatc
    33901 aagcgcgagc atccgggcga cgcgatcctg gccgaggagt ccggcgagca cagcggggcg
    33961 ctgcgtgacg acgggacgca caacggccgc tgctgggtca tcgacccgct ggacgggacc
    34021 gtgaactacg ccaacggcat cccgtactac tgcgtcagca tcggcctggt ggtggacgac
    34081 cgcccggcag tcggcgtcat cttcgacccc gcgcgcggcg acctgtacac ggcgaccgcc
    34141 gatggtccgg cgctcctgga cggggacccg atcgaggcct ccaccaagga gaccctcacc
    34201 gactacgtgg tcagcctggc cgtcatcggc cgcggtgggc tcgcccggga acggcggatc
    34261 gcgccgcgca tccggatcca tcggcgcatg ggcagcgcgg cactgtcgct ggcctacgtc
    34321 gccaacggcc ggttcgatgc cttcgtccag aacggcggcc tctccccctg ggacgtggcc
    34381 gcggcggggc tcatcgccga gcggtcggga gcggtcgtga cctcgatcga cggcggtgcg
    34441 tggtggaacc cgaaggtgcg tggcccgcgg accagcgtgg tggccgcccc ggccgcccag
    34501 caccaggcgc tgctcgagct cctcggctcg ctcaagacga cggtccagac gcgccgctag
    34561 ggagtgcccg ccgcggcctc cagcacgtgg gcgaacatgc cgagcgtgag ggccgtggcg
    34621 ccccacacca ccagctcgcc gtggcggtac gcaccggcgc ggaccgtgac gccccgggcg
    34681 tcgaactccg cgtcggcgag caccgcgggg tcgaagagcg cgcgaagcgg cagctcgacg
    34741 atcgccgcca cctcggcatc atgcgggacg agcggcggcc gccgcgccac ggccccgacg
    34801 aagggctcca gctcgtagtt gctgaccggg atccagaccg gatccagccg cccgagcacc
    34861 cgcaccagcg agggttcgag gccgacctcc tcccacgcct cgcgcagcgc ggccgcctcg
    34921 gcggaggcat cggcggcgtc gatcgcgccg ccgggcagcg agacctcgcc ggcgtgcgcg
    34981 cgcagctcgg atcggcggat ggtcagcggc acgaccaggt cgccatcctc atcggggtac
    35041 agcgccagca gggtcgcggc cggtcgcgcg ggcgggaagc cgctgcggtc gtatcggcgc
    35101 ctggtggtct ggccgtcgag ggtgcgcggc aggagccgct cgtcgagggg gatcggcctg
    35161 gcgacgagcc ggagggcttc ggcgacctcg tcctcccacc cggcgtgagc gcgtagtatt
    35221 cgctccatga cccaggctca cctcgatcgc gacagccgcg tgcggagcgc actctaccgc
    35281 acgcgcatcc tggtgaaccg cagcccgcac ctgaccgatg cgacgcgcaa ggaggccaac
    35341 cgcgcgctcg acatgctcga ggggcagctc ggcctcaacc aggtcaaccc cgtcgatgcc
    35401 gcacggggcc tcgagctgct gaaccgcgcg cactcgtcgc tgctgttcgg gctgctgcgc
    35461 aacacggatg tcgcggaccg attcgcgccg gcactccgtc gccttggcct gcgcggcatc
    35521 accgaccggc tggacgaggt gccgcactcc gtgatggccc tgccgatgcc cgggccgtcc
    35581 ggcggccgtc ggcatcgcga cgacctgccc ccggacgagc gcctggacgc ccacgggaac
    35641 ccgctgccgc cgcctccggg ctactgaccg gcggacgcct cgcgcgtcgc cagcgcctcc
    35701 accagcgcgg catccgtcgg cgtcgagccg acgcccagcc tctctccgac gaccgccgcc
    35761 agcggtggct cgaggtacgt cgaacgcagc gcctcccagg cggcagccgc gaacgcctcc
    35821 gacggagccc cgtccaggac gatgcgtccc gaacgcatca ccaccacccg ctcgaaggtc
    35881 tccgccacga acgccatgtc gtggctgatg gcgaggacgg tgcgtccggc ggcggcggcc
    35941 gcgtggatca cctcccgcac gcggtccacg ccccggcggt cctggccggt cgtcggctcg
    36001 tccagcacca gcacctcggt acgcatggcc aggaccgatg cgagcgccag cagcttccgc
    36061 cgtgactgtc cgagatcgta agggttcgtc cgctcgtcac cggtgaggcc ggaggcgacc
    36121 acggcctcct ccacggccgc gtgcagcgcc gccccccgca gcccgaggtt gcgcggcccg
    36181 aaggcgactt cctcgtgcac ggtgccggcg aacacctggc ggtccggatc ctggaagacg
    36241 agcccgacgt ggcgggccag ctgggcgacg gtgagcgttg cggcgtcccg cccatcgatc
    36301 gtcacgcgcc cctcggtcgg ccgcagcagg ccgttgaggt ggcggaccag cgtggtcttg
    36361 ccactgccgt tctgtccgac cacggccacg cgctcgcccg gaccgatgcg caggtcgacg
    36421 ccgtcgacgg cccgaacgcc gctggacgcg tacacgtgga ccaggcgctc gacgacgagg
    36481 tcagccactg atggcggcct cgacccgatc cggcgcaacg ccggcgtcca cgaccgcgcg
    36541 tcgcaggcgc acggccgagg gctccgggac ccccagctcg gccaggcgcg ggtcggagag
    36601 gacggcgtcg gcgtcgccct ccagcaccac ccggccgccg tcgagcacca cgacgcgacc
    36661 ggccacggcg gccagcaggt ccgtcttctg ctcggccacc aggatgctcg ccccgtcccc
    36721 cgccagccgc tggatcgcgt cgcccacgag ctgcgtcccg gccggatcga gctgggccgt
    36781 gggctcgtcc aggaccaggt gctcgggccg catcgccagc aggccggcga tggccaccag
    36841 ctgctgctgt ccgccggaga gccgggccgg gtcgcgctcg gccaggccat cgatccgcag
    36901 ggcggcgagg gcctcccagg tgcgctgcgc cacctcgtcg cgcggcaccc cgaggttcat
    36961 cggcccgaag gcgacctcct cgaagaccgt gccggccacc tgcgacagct gcgtcgtcgg
    37021 gttctggaag ccgatgccga cgcgggacgc cagccgatgc atcggccagg cgtcaacgtc
    37081 ctcgctgtcc agggtgatcg tgccacggat ccggccgccg accgtccgcg gcgccagtcc
    37141 gctgacgacc aggcacagcg tcgtcttgcc cgactcggac gccccggcca cgccgaccac
    37201 cgcgccgtcg ggcaggtcga ggtcgacgtc cagcagggcg gcctgctggg caccagcgta
    37261 ccgatagccg acgccgcgca gcgagatggc catcaggcga tcccggccac gcggacgatg
    37321 atcagcgcga gcagcgcgaa caggagcgcc cagcgaaccg cgcgctccca tcccgtgtcg
    37381 ggcggcgccc acagcagggt ccgtcgcccc ggccgcgtga agccgcgcgc ctccagggcc
    37441 atggtgcgct cctcgacctc ggcgatcgcg ccgaggatca ccgggccgac gatcggcacg
    37501 atcccgcgca cccgccgcca caccgacccc tcggtgtcga ggccgcgcgc ccgctgcgcc
    37561 gcggtgatct gggcggctcg ctccaccatg gccggcaccg tctgcaccga tgcgttcgcg
    37621 acgaacccga cccgcgagct gacgccgcgc cgctcgaggt cgaccaccag gtccgccggc
    37681 cgcgtggtca ggtagaagag cgtcaccgcg ccgctgatgg ccaggatgcg gaccaggatc
    37741 tcgagggcga acgccagccc ctcggccgtg gcgacgaccg ggccgatccg cagcagctcc
    37801 gtcgtgccgc cggggaagaa gaagaggttg accaggaacg cggagaccgc gatcggcagg
    37861 gccagcagca gcgccgtcct cagcaggcga cccaggacgc cggcgagcag gccggggagg
    37921 aggacggtga ccgtgacgag caggatcggt ccgaccaggc cgcccaggac gacggccgcc
    37981 accgccgtga cggtggcgag gaccgccttg gtgagcgggt tgagccggtg gaaggcgctg
    38041 gggccgggac gcccgaggaa gtcggggacg ccccggctcg agagggacgg atcggtcagc
    38101 tcgttgcgct ccgccactca ccctcctcga tcccgccggt gtctccggcc tcgaggagct
    38161 gctcgccctg cgggaagcgg gccttcgtgc gccgcgccat ggcgttgagc accaggtaga
    38221 cgacgaagaa ggtgaccagc ttgtcgaccg ggtccgacag cagcccctgc tggaaggtcg
    38281 ccgtctgcag gtccgagccg gcctgccgga acgccgcgac caggaagtcg gtaccgcctc
    38341 cggtgacccc gctgaagacg ttggcgctga tcggtgcgct gatcagcgcc gcgatgacgc
    38401 cggtgaccat cccggccacc accacgtagg caacgcccag gtcgcgccgc gcgaccagca
    38461 ggtaaccgaa gccgacgacg aagccgacga agatcaggcc caccagccag cccaggacga
    38521 cgaacagcgg ctcgacctcc acgtcgggga tgaagaagtt gaacgtgctg ccgtagaagc
    38581 gggtgtaggc gtagaccgcg agtccgacga ccacggccag cgccaccagt ccgccgatca
    38641 gcagctcggt cccggatcgg ttcggccgtg gccgcagcca gcccgctcgg ccggcgaagc
    38701 cggcgagcac gccgatgacc gccgcgacga gggcgaaggc cgccgcagga ccgttctgga
    38761 agggcggtgg caggacgtag gtccacagga tgttggtcag aaagccggtg aaggcgccgg
    38821 cgaccggtcc ggccaaggcc ccgaccagga tggtgccgat gctgtcgagg tagacgggaa
    38881 tcttcagggc gttcgcgacg gtggcgccca ggatgacgtt gatggcgatg gcgatgggca
    38941 tgagcacgat cgtccgcgtg tcgaactgcc gcgcgatcga gccgaaaccc ccggtctggg
    39001 cgtactcgat ggcggcgtag accaggaacg cgacgaccag cgcgatggca ccgatgaacg
    39061 cgaacgaatc ggacccctcg gtgtcgaaga agttccccgg atcgatgccg gtgtcgaagt
    39121 tgccgacggt gacgacgccg atcaggatga ccgccacgaa cgccacgagc gcggcgatcc
    39181 agcgtacgag catggcgaga accctcctcc gtgcggggcg tgcccctagc tgtagggagc
    39241 gctgacggcg cgcgccaacc gcgccagcag ctcggccttc atcccctctc catcgatggc
    39301 ggtcgcgacg ccgaccgggc gccgtccgtc ctcggaccga tacaggctgc ccgcgatgta
    39361 cggatccgag ctcgtatcgc aggcgagctg ctcactgacc gaatcggtca ccaggtccgg
    39421 ccgcagcagc gagccgacca gcacgaccgg gtcgtgcagg taggcgccgt cgatcccgtc
    39481 ggcacgctgg tggaaggcga cgtagaagcg caccgcgtcg cgaacgatgc cggccaggtg
    39541 cgtatcgggc agggcgcgca ggtcgtccat ggtgaagacg acgtcctgcg tcgcgtcgag
    39601 cgggaagatg cggtgcaggg caccggccgc caggcacacc tcggccgcct cgggatccac
    39661 gcacgcgttc cactcgcccg tctcggtcgt gtttccgcgc tcctcgagcg tgccgcccat
    39721 ccagatcacc tcgcgggtcg agcccgccag gtcgaccccg tgctggaccg cggccgccag
    39781 gttcgtcagc ggcccgagcg cgacgatggt catctcgccg gggtggcggc cgacgtgcgc
    39841 gccgaggtag cggctggccg ggatggcggt gtactcggtc gtcccaagct gccggtcctc
    39901 gccccgcagc tcgacgtagc cggtgcccgt cgggccgtgc gtgtccggcg cggttcgcag
    39961 gcgacgctgc agcggccggg ccgcaccgat gctgaccggg acgtcaccgc gcccgaccat
    40021 acgcaggatc ttgccggtgt tgcgcgccac gtggtggatc tcgacgttgc cggcgacgca
    40081 ggtgacgccg cgcaggttga gctccgggct ggtggccgcg atgagcagcg cgatcatgtc
    40141 gtcgatcccg gtgtccacgt ccaggatcaa cgggcggggc gcggcggtct ctcccgaccg
    40201 agcggcgtcg gcgtccatcg gccgcctatg gtacgcgcca gccgccggcc tggccgacct
    40261 tgctggccag gggccggccg agcgtgtcgc tgatctcgcg cgctgccacc agcagcgcgt
    40321 ccaggtcgac gttgtggcgg catccctcgc ggtcgagcag gtacacgaga tcctcggtgg
    40381 ccaggttgcc ggccgccccg ggcgcgtacg gacagccgcc ggtcccgccg gtcgaggagt
    40441 cgaagcagcg gacacccagg tccatggcga cggcgacgtt cgcgagcgcg gtgccgcgcg
    40501 tgtcgtggaa gtgcatggcc aggcggtcgg gactgatgcc ggcgttgagc agggcaccga
    40561 ccacgcgacg cacgtccgcc ggacccgcca cgccgatggt gtcgccgatg ctgagctccc
    40621 cgacgcccag gtccagcagc tgctgcgcga cgcccacgac ggcggcctcg ccgacccggc
    40681 cctggtacgg gcagccgaag gcggtgctga cgtaacctcg cgtccaccag ccgcgctcct
    40741 tggcgcggtc gatgaccggc cggaacgcgt cgatcgactc ggcgatcgtc atgttgatgt
    40801 tcgccttcgc gaacggctcc gacgcggcgg tgaagacgca gaccgcctcc accccggccg
    40861 cctcggcccg ctcgaggccg cgctcgttcg ggacgagcac cgggtagcgg acgccggggc
    40921 gccggtcgag gcgcgccatc agcgcgtccg cgtccgccag ctgcgggatg gccttcggcg
    40981 agacgaagct ggtcgcctcg atctcgcgca ggccggcatc ggcgagcagg tcgatgaagc
    41041 gcagcttggc gtcggtcggg atcggcgtgg cttcgttctg caggccgtcg cgcggaccga
    41101 cctcgaagat ccggacgtcg cggtcaggca tcgccgccat ggtacgcgcc ggccggcgtc
    41161 acctcggcca gcaggtcgcc gcgctgcacc tgctgccctt cccgaaccat cagacgggtg
    41221 agtgtgccgt ccgtggccgc gaccacggcg tgctccatct tcatggcctc gatcaccacc
    41281 acggtctccc gcgcgtgcac cgatgcgccc tcgcgggcgc ggatggcgat gacccggccc
    41341 ggcatcggtg ccgtgagcag ggcgccctcg tcgccggatg cccgcgccgc gtgccgcacc
    41401 gcctcctcga tcgtcggggg tggcgcgatc cggaactcga gcgactgccc gtcgacgtcc
    41461 acgaaggcgc tggcgccgac ccgagccgcg gagggcggat cgccgctcgc gtcgagcgag
    41521 accgttcgct cctccccgtc gtgcgccagg cgcaggacgc gcggcgcgtt gaggcgccaa
    41581 ccgccggacc agagcccccc ggcgtggtcg aggacccggg ccgcggcccg ccagtgcgcg
    41641 tcgccgggga ccggcgcgtg cgggagggcc atgccggcca ccgtatcggt ccgcatctcg
    41701 ccggcgcgca tcggctcctg gcgcagcagc cagcgcagga agcgcacgtt cgtccgcaca
    41761 cccaggatgg tggtctcatc cagcgcggcc atgagccgat cgagcgcctc ggcgcgggtg
    41821 cgtccgcagg cgatcacctt ggccagcatc gggtcgtagc ggtccgtcac ctcgtcctcg
    41881 gcggccaccc cggtgtcgac gcgtaccgcg tccggccatc ggaccatggc cagccgcccc
    41941 gtggccggga ggaatccggc ctcgggatcc tcggcgtaca cgcgcgcctc gatggcgtgg
    42001 ccgcgcagcg ttggcggggc ggacagcccg agctcgtcca gcgtggcgcc ctgcgcgatc
    42061 cgcagctggt cggcgaccag gtcgcggccg gtgacctcct cggtgaccgg gtgctccacc
    42121 tggaggcggg tgttcatctc gaggaagaag aactcgccgg agtcggccag cagcatctcg
    42181 accgtcccgg cgctgacgta cccgacggcc gctgcggcgg cgacggccgc cgtgcccatc
    42241 cgctcccgcc gtgccgcgtc cacggcgggg gcgggtgcct cctccaccac cttctggttg
    42301 cggcgctgcg tgctgcagtc acgctccccc aggtggacac cgttgccatg tgcgtcgaac
    42361 aggacctgca cctcgacgtg acgcgagccg gcgaggtagc gctccagcac cagccggtcg
    42421 tcaccgaagg accgatgcgc ctcgcgccgc gccgccgcca gcgcctccga cagctcgtcg
    42481 gcggtgcgca ccacccgcat gcccttgccg ccgccacccg cgctcggctt caccagcagc
    42541 gggtagccga ttcggtcggc ctgctcggcg agcgtcgtgt cgtcctgcgc ctcgccgtcg
    42601 tagccgggga tcgtcggcac gccgtgctcg gagacccgct gtctcgcggc cgccttgtcg
    42661 cccatcgccg cgatggccgt cgcggggggc cccacccacc ggatgccggc gcgatcaacc
    42721 gattcggcga acgcggcgtt ctcggccagg aagccgtagc cggggtggat ggcatcggcg
    42781 cccgccctgc gcgccacagc caggatcgcc tcgccatcga ggtacgagtc gatctcgtgg
    42841 ctctcgtcgg cgccatgcgc cggtcgctcg tcggcggcgt ggacgccgat cacccgaatg
    42901 cccagccggt gcgccgtccg cgcgatgcgc acggcgatct cgcctcggtt cgcgatgagc
    42961 agtgagcgaa tgggtgtcgt gctcgccggg gggcccgctg tgccgaccgc agaaagcgac
    43021 gaacgcgaca ctcgaacgcg cgcgccgacc tgtcgcgccg ggagtcgacc gctggagatc
    43081 cagcgctgga ccgttcgcgc cgtcacgccg agctcgcgcg cgacctggct gggcgtcagc
    43141 tcctcgtcag ccatgtcgcg attgtcgcac taccagcgcg gccggcgctt ctcgacgaag
    43201 gccgtgaggc cctcctggcc ctccgcggac gtgcgttgcc gggcgatgcg gcgcacggtc
    43261 tcggcccgcg cctcatccgc cggtcgaccg cgcaggtcgc ggatgagcgc cttcgccgcg
    43321 cgcgccgcct ccgggccggc ggcgtcgagc tcggtctgca gtgccgtcac gcgctcgtcg
    43381 agggccacct cgtcggggac gacctcgtgg acgagaccga tccgctgccc gcgttccgcg
    43441 tcgaagcgct gtccggtggc gaagagcgcg cgggcatgtg actcgccgat cttcggcagc
    43501 acgaacgtgc tgatcaccgc tggaatgatg ccgagccggg tctccgtgaa cccgaaggtg
    43561 gtgtcggccg tcgcgaccac cacgtcgctc acggcgcaca gccccatccc gccacccagg
    43621 gccgcgccct ggacgcgggc aatcaccggc accgggcacg cgtcgatgac ggcgaacatc
    43681 gcctgcatcg cggcggcgtc gcgttcgttg tcctcgacgg agaggccgat ggcggagcgc
    43741 atccagtcga tgtcggcgcc ggcacagaac gagcggccct caccggccag caccacgctc
    43801 cggagccggt cgggcggctc ggtcgcgagc gcctcgaagg tcgtgcgcag ttcgtcgatc
    43861 agcgcggcgt tgaacgcgtt gcgcacctcg ggtcgggtca ggacgacgcg cgcgtgtaca
    43921 ccatcgcgtt ccaggcgcag cagcccgctc accggccacg cccccgtcgg tggaacggct
    43981 cagcgacggc ccgcacctgc gccagcagcg ggtcctccag ccagacgaca tcggtgccgt
    44041 cgggcacatc gacggtggcc gtcgttccgg gctcgccaac cagacccaac agcagccgcg
    44101 ccgatcccga tccgtcatcg gtctcgacca ggtaggcggc gtctacggcg ccgccgcgga
    44161 gcgcatcggc cagggagcgc tcgagatcgc gggtatcggt ggctggaagg cgtacccgga
    44221 tggacgcgcg ggcggtcgga gcgaagaggg ggtcgtcggt gcccgggatg cggccctcgg
    44281 ccagggcgac cagctcctcg gcgtcgaagc gatacgggac cggcccggct gggtccatga
    44341 cggcgggcag cccggccgcc gccaggttgc cgatcgcgat ccgcgcgggc agcgggaggt
    44401 tgccggtgtt ggccggcgcc cacgccgcca gtgccgcgaa ggagccgaag acgacgctca
    44461 ccggctgctc gccctcgcgc gccagcatca cgctgatctc gacctgctcg tcgtcgttga
    44521 ccgcggcggc gagcgcctcc ttcgcctcct cgccgtgtcc cggcggaacg ggcaacagca
    44581 gcgtgccgct catcagcgtt cggtagaact cgtggagcgg ggcgctgtcg cctcgtgcct
    44641 ccgcgtcacg cgcggccagc atcgcgtcga acaggttcgc ggcgctcgtc tcgagcgagc
    44701 cggagggcgc gctgcggtcg tgggaacccg gcgtcggccg gtcttcggca ggctggtgca
    44761 tgggtccggt gagcctaccg gatgctggta gcatcggcga catgatccgg ggcatcgacc
    44821 acctggtcgt cgccgtcacc gatccggacg ccgctgccgc cgagctcgag tcacgggtcg
    44881 ggctgacgtg caccggcggt gggcagcatc cgggtgccgg cacggtcaac cgcctcgcct
    44941 tcctggccga tggcagctac ctcgagctca tcggcgtgga ggacccggcg gccgcgcgcg
    45001 ccaacccggt cggcgcggcc tcgctccaga tgctggagac gaacggcgct gggctggcga
    45061 ccttcgcgct cctcgacgac gacctggagg cgaccctgcg gtctcgtccc gagctcggga
    45121 cgcggaccca tggctcgcgc caacggccgg atggcgaggt ggtggagtgg tggacggccg
    45181 cgcctaccga gccgttgtcg ccgacctcgc cgttcctgat cgagcacacc tacgccggtg
    45241 cggagtgggg tcccgaggcg atgcaggttc gcgccgagca ggtccatccc atcggctcgg
    45301 ccgtgcgcct ggtgcgtctt gacatcgcgg tcgacgatcc gccgctcgtc gccggcgagc
    45361 tcgcgtccat cctcgacgtc cccgtgcagg ccgt
//
LOCUS       DASTYP010000065         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1534280, whole genome shotgun sequence.
ACCESSION   DASTYP010000065 DASTYP010000000
VERSION     DASTYP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534280"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /gene="purH"
                     /locus_tag="VFV59_03390"
     CDS             <1..699
                     /gene="purH"
                     /locus_tag="VFV59_03390"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU5324906.1"
                     /translation="DLAEAFRLALEGDSVSAYGGIIGCNRIVDESTARAIRPGFFEAI
                     VAPGYTAEALEILRTKRGFEIIEVPPAANDEDDLGIGKFDFKRIGGGLLVQTFDEMEE
                     DRDKLQTVTQRRPTLEELTDLLFAWRAVRHVKSNAVVLAKKHAMIGMGAGQPSRVVSV
                     EVALRKAGERAPLSVMASDAYFPFPDGIQIAAQAGVTAIIQPGGSIRDEMAIEVADRH
                     HMAMVFTGRRHFKH"
     gene            724..1713
                     /gene="glpX"
                     /locus_tag="VFV59_03395"
     CDS             724..1713
                     /gene="glpX"
                     /locus_tag="VFV59_03395"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEU5324907.1"
                     /translation="MTAGMDKLIIAEAVRATEAGAIAAARLMGRGDRDGADQAAVTAM
                     RKAMDEADISGTIVIGEGERDEAPMLYIGEEVGNPDAAVAIDIAVDPLEGTNLVATGS
                     PNATAVLAAAEPGGLLHAPDTYMEKLIVGPSVAGRVSIDDPVEKTLHTIADALGRYVW
                     DITVVILDRPRHEKLIADVRSAGARIKLIGDGDLTAGINAAVSGTGIHAVMGTGGAPE
                     GVLAAAALKCLGGEIQGRFRWRSEEERDRARAMGVDVDDSDRIYTTNDLASGQRVVFC
                     ATGVTDGELLRGVRFFGGGARTHSILMSYRQGIVRFIDTVHMWDPGNPPKLRL"
     gene            complement(1770..2309)
                     /gene="folK"
                     /locus_tag="VFV59_03400"
     CDS             complement(1770..2309)
                     /gene="folK"
                     /locus_tag="VFV59_03400"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU5324908.1"
                     /translation="MRAFIGIGGNLGNVAATFRTAASELRAIGQVVRVSSMYEGAPRD
                     AFDQPRFLNAAIEMEVDLEPHDLLVELKRLELSLGRDPQGRRFGPRVVDLDILLIDGR
                     CVTDVEHDLIVPHPRLHERRFALEPVAELDPGLHPWRGQGCADLRIDVSVADLLATVA
                     GQDVVRVGGPEWADPVSAD"
     gene            2486..>4053
                     /locus_tag="VFV59_03405"
     CDS             2486..>4053
                     /locus_tag="VFV59_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324909.1"
                     /translation="MSDIKYTYPLILNKVASPQYATPTLRRARLIDWLNEAADCRAAV
                     IAADAGYGKTTLLWQWEREVRFPVYWYKLDRNDRDWTFHISYLIEAISKRHRGFGRRA
                     HSMLQQLGGPGSSRPGVAAYLLAEMHERLTEPCTFIIDDWQYVNAVTEVRGLWNQILR
                     DAPATCRFVFASRVKPRLQFARFKTHGGYAELRTDALRFTEPEIDELFRDIYNDPLEP
                     QDVAELERRTEGWAASLQLVEVSLRERQTVEERRAFIQSITATKDSDLFDFLAEEVLD
                     QQNEETRNFLLSTSILQQITPEVAERLTGVHDGRRELTELEHRGLFTNRLDDIRFRYH
                     NLFREFLERRLVAERTDAEVVGLHIHAASYFETTEQWPEAIHHYLRAGLQRQAARLIA
                     KYGEEVVAEGRLGLVDEWLQALPDDTIKQNARLSLLFGETCGIRGEWERALESLERAR
                     GFFARKGDRRLESLACLKLSSVYSNYGDTDAALQAAEDGVALVPPDAVATRLRLEGNI
                     AITRTWLNEPLEAVV"
BASE COUNT          654 a         1462 c         1382 g          555 t
ORIGIN      
        1 gacctggcgg aggcgttccg gctggcgctc gagggcgaca gcgtcagcgc gtacggcggg
       61 atcatcggct gcaaccggat cgtggacgag tcgacggcgc gggccatccg ccccggcttc
      121 ttcgaggcca tcgtcgcccc gggctacacc gcggaggccc tggagatcct ccgcaccaag
      181 cgtggcttcg agatcatcga ggtcccgccg gccgccaacg acgaggacga cctgggcatc
      241 ggcaagttcg acttcaagcg cattggcggg ggcctgctgg tccagacctt cgacgagatg
      301 gaggaggacc gcgacaagct gcagaccgtc acgcagcggc ggccgaccct ggaggagctg
      361 accgacctgc tgttcgcgtg gcgcgccgtg cggcacgtca agtcgaacgc cgtggtgctc
      421 gccaagaagc acgcgatgat cggcatgggc gccggccagc cgtcacgcgt ggtgagcgtg
      481 gaggtggcgc tgcgcaaggc gggcgagcgg gccccgctgt cggtcatggc gtccgacgcc
      541 tacttcccgt tcccggacgg catccagatc gcggcgcagg ccggggtgac ggccatcatc
      601 cagcccggcg gcagcatccg cgacgagatg gccatcgagg tcgccgaccg ccaccacatg
      661 gcgatggtct tcaccggccg ccggcacttc aagcactgat cagcgcgacg gattgacgga
      721 ccgatgaccg cggggatgga caagctgatc atcgctgagg cggtgcgcgc caccgaggcc
      781 ggcgcgatcg ccgcggcgcg cctcatgggt cggggcgacc gcgacggcgc ggaccaggcc
      841 gccgtgacgg ccatgcgcaa ggcgatggac gaggccgaca tcagcggcac catcgtcatc
      901 ggcgagggcg agcgcgacga ggcgccgatg ctctacatcg gcgaggaggt cggcaacccc
      961 gacgcagcgg tggccatcga catcgccgtg gacccgctgg aggggacgaa cctggtcgcc
     1021 accggctcgc ccaacgcgac cgccgtgctg gcggcggcgg aacccggcgg cctgctgcac
     1081 gcgcccgaca cgtacatgga gaagctgatc gtcgggccga gcgtcgccgg gcgggtgagc
     1141 atcgacgatc ccgtcgagaa gaccctccac accatcgccg atgcgctcgg ccgctacgtc
     1201 tgggacatca ccgtcgtcat cctcgaccgg ccgcggcacg agaagctcat cgcggacgtc
     1261 cggtccgccg gcgcccggat caagctcatc ggcgacggcg acctgacggc cggcatcaac
     1321 gccgcggtct cggggaccgg catccacgcg gtgatgggca ccggcggcgc gcccgagggc
     1381 gtgctggcgg cggccgccct caagtgcctg ggcggcgaga tccagggccg gttccggtgg
     1441 cggtccgaag aggagaggga tcgcgcgcgt gccatgggcg tcgacgtcga cgacagcgac
     1501 cggatctaca ccaccaacga cctcgcgtcc ggccagcgcg tcgtcttctg cgccaccggt
     1561 gtgaccgatg gcgagctcct gcgcggcgtc cgcttcttcg gcggcggcgc tcgcacgcac
     1621 tcgatcctga tgagctaccg gcaggggatc gtgcggttca tcgacaccgt ccacatgtgg
     1681 gacccgggca acccgccgaa gctgcgcctg tagcctctcg cgtcccttcg ccgatacgcc
     1741 cgccgggcgg gaatgcctgc ggggaccgat cagtcagccg agaccgggtc cgcccactcc
     1801 ggcccaccga cccggaccac atcctggccc gccaccgttg ccagcaggtc cgcgaccgac
     1861 acgtcgatcc gcaggtcggc gcaaccctgc ccgcgccacg ggtgcagccc cggatcgagc
     1921 tcggcgacgg gctccagcgc gaagcggcgc tcgtgcaggc gcgggtgcgg cacgatcagg
     1981 tcgtgctcga catcggtgac gcatcggccg tcgatgagga ggatgtcgag gtcgaccacg
     2041 cgcggcccga atcggcgccc ctgcggatcc cggccgagcg acagctccaa acgcttgagc
     2101 tccaccagca ggtcgtgcgg ctcgagatcg acctccatct cgatggcggc gttcaggaag
     2161 cgcggctggt cgaaggcgtc gcgcggcgct ccctcgtaca tcgacgagac ccgcaccacc
     2221 tggccgatcg cgcgcagctc ggaagcggcg gtccggaagg tggccgccac gttgcccagg
     2281 ttcccgccga tcccgatgaa cgcacgcacg ctgcgcgcag cgtagccgat ccaccacccc
     2341 ttgcccgatc gcgccatccg ccacggacct ttccatcggc cggtaagcca ccgataatcg
     2401 gcgctcggta ggatccgtat cggctgcgcg ttcgcggggg gcgcgacgac ggtctgacgt
     2461 ctctcgcccg cccggagacc gtggattgtc ggacatcaag tacacctatc cgctgatcct
     2521 caacaaggtc gcgtcgccgc agtacgcgac cccgacgctg cgccgggcgc ggctgatcga
     2581 ctggctcaac gaggcggcgg actgccgagc ggccgtgatc gccgccgatg ccggctacgg
     2641 caagacgacg ctcctgtggc agtgggagcg cgaggtccgc ttcccggtct actggtacaa
     2701 gctggaccgc aacgaccgcg actggacgtt ccacatcagc tacctgatcg aggcgatcag
     2761 caagcgccac cgcggcttcg gtcggcgtgc ccactcgatg ctccagcagc tgggcggacc
     2821 cggctcctcg cgccccggcg tggcggccta cctgctggcg gagatgcacg agcggctgac
     2881 ggagccatgc accttcatca tcgacgactg gcagtacgtc aacgcggtca ccgaggtgcg
     2941 cgggctctgg aaccagatcc tgcgcgacgc gcccgccacc tgccgcttcg tcttcgcctc
     3001 gcgcgtcaag ccgcgcctcc agttcgcccg cttcaagacg cacggcggct acgccgagct
     3061 caggaccgat gcgcttcgct tcaccgagcc ggagatcgac gagctcttcc gcgacatcta
     3121 caacgacccg ctcgagcccc aggacgtggc cgagctcgag cggcgcacgg agggctgggc
     3181 cgcgtcgctt cagctggtcg aggtctcgct gcgtgagcgg cagaccgtcg aggagcgccg
     3241 cgccttcatc cagtcgatca cggcgacgaa ggacagcgat ctgttcgact tcctggccga
     3301 ggaagtcctc gaccagcaga acgaggagac gcgtaacttc ctcctctcta cctcgatcct
     3361 gcagcagatc accccggagg tcgcggagcg gctgaccggc gtccacgatg gtcgccgcga
     3421 gctcaccgaa ctggagcatc gcggcctctt caccaaccgg ctggacgaca tccggttccg
     3481 gtaccacaac ctcttccgcg agtttctcga gcgacgcctg gtggccgaac ggaccgatgc
     3541 ggaggtcgtg ggcctccaca tccacgccgc ctcgtacttc gagaccaccg agcagtggcc
     3601 ggaggccatc caccactacc tgcgcgcggg cctccagcgc caggcggcga gactcatcgc
     3661 gaagtacggg gaggaggtgg tcgccgaggg acgactcgga ctcgtcgacg agtggctcca
     3721 ggcgctcccc gacgacacga tcaagcagaa cgcccggctg agcctgctct tcggcgagac
     3781 gtgcggcatc cgcggcgaat gggaacgcgc gctcgagtcc ctcgaacgcg cccgcggctt
     3841 cttcgcccgc aagggcgatc ggcgcctgga gtccctcgcc tgcctgaagc tgagctccgt
     3901 ctactcgaac tacggagaca ccgatgcggc cctccaggcc gccgaggacg gcgtcgcgct
     3961 cgtcccgccc gatgcggtgg ccacgcgcct ccggctcgag ggcaacatcg ccatcacccg
     4021 cacctggctg aacgagccgc tcgaggcggt cgt
//
LOCUS       DASTYP010000066         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1643997, whole genome shotgun sequence.
ACCESSION   DASTYP010000066 DASTYP010000000
VERSION     DASTYP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643997"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFV59_03410"
     CDS             complement(<1..554)
                     /locus_tag="VFV59_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5324910.1"
                     /translation="MNPHQAAASVRDALDRPLHDLRVSVTDRCNFRCTYCMPREVYGV
                     DHAFLPRAEILDFEEIARVVAAAVTLGVRKVRLTGGEPLVRRDVERLVAMLRQIPGID
                     DLTMTTNGSLLRSKAAALAAAGLDRVTVSLDALDDPTFLRMNDAGIPVARILDGIAAA
                     QEAGLGPIKVNAVIRRGINEHAIC"
     gene            complement(611..1285)
                     /locus_tag="VFV59_03415"
     CDS             complement(611..1285)
                     /locus_tag="VFV59_03415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5324911.1"
                     /translation="MTTRADRSRGMPRRTTVETAPRTNRTPLLLVGGGILLLVVVAAV
                     AALLLSGGGGGLREPATTPIAISGNPLPAYDPNTSDTALGQPIPTLTGTDLSAQPMTI
                     GPDQGPMAIVVLAHWCHVCQAEVPQLVDWLSTNQVPEGVKIVTLTTSITPTAQNYPPS
                     TWLDREGWTAPTMVDDGNSRGLDALGISAFPGFVFVDADGRVVQRVTGQLPAETFGQM
                     LQAIAP"
     gene            complement(1282..1875)
                     /locus_tag="VFV59_03420"
     CDS             complement(1282..1875)
                     /locus_tag="VFV59_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEU5324912.1"
                     /translation="MSVEVVSTFLALLALGAGFGVLATLTLWVLDRIGLVPGAMDGFT
                     ELVRPAALWLAFLVAAVAMAGSLYFSIGAGFVPCDLCWYQRIAMYPLAPILAVAALRR
                     DPRVAWYAVPVALIGAALSIYHIGVERLPGMPSAWCSLQSPCNVIWVERFGFVTIPTM
                     ALLGFLAIVTLLLVYARPAPAAEETRDHLITNTEPIS"
     gene            complement(1995..3419)
                     /locus_tag="VFV59_03425"
     CDS             complement(1995..3419)
                     /locus_tag="VFV59_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5324913.1"
                     /translation="MTATEPAPVHGSELVRLVGEEVAVPLVTGETRRYVNLDYAASAP
                     ALRTVHDAVEELLGWYSSVHRGAGFKSRVATTAYEGARESIRRFVNARDDDAVIITRN
                     TTDSINLLAGTLPSGTHVVCFAGEHHANLLPWKRIGVRYLPVPESPAQMLETLEATLR
                     ALPTCGDPHLVAVTGASNVTGEIWPIAEVARIAHDNGARLLVDAAQLAPHAPIDMQRD
                     GIDYLALSGHKLYAPYGAGALIGERDWLGAGDPFLRGGGAVKLVTVDETLWADLPDRQ
                     EAGSPNVVGAVALGVACETLASVGMQRVADEERALLAYARERLRDVPGFRHYRVWSTD
                     HPRVGLMTFSLGSVPYDLLAAVLSAEHGIGIRHGCFCAHPLMMRLLKVDDAEAHRLVE
                     ETRAGHHERLPGAARMSLGLGSSRDDVDALADALRAIATDGPRWTYAVNPNTDEYEPD
                     PDPRPLPALRMRLVDHPHGHGESA"
     gene            complement(3441..4181)
                     /locus_tag="VFV59_03430"
     CDS             complement(3441..4181)
                     /locus_tag="VFV59_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006909098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5324914.1"
                     /translation="MELDALLLALLFFGAALLYASVGHAGASAYLAAMALVGVAPATM
                     RPTALVLNLFVASIVVVRFARAGHLPWRQLVPLAIGSIPMAFLGGSIELPGEVYRPLV
                     AVVLLAGAWRLATASTTDGEPERPAIPPLAGVASGAGIGLLAGLSGTGGGIFLTPLLV
                     LTRWTGVRDAAGLSGAFILVNSLSGIAGLLTIGFVLQAAMPIWVLAVVAGGVLGSWLG
                     AHRFTILGLRRVLAFVLVLAAAKLVFLP"
     gene            complement(4194..4742)
                     /locus_tag="VFV59_03435"
     CDS             complement(4194..4742)
                     /locus_tag="VFV59_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5324915.1"
                     /translation="MTDRIADTLALLRRTPELLTALLAGLPEAWTDTPDTPDGWRPRD
                     VVGHLITAEVDDWLPRTQRILEHGTAVPFDPMDRTAMFERDADATLDELLDRFAALRA
                     ENLGRFEALAHEGDLDRQGMHPALGEVTLGNLLATWAVHDLNHLGQIVAGLAGSHDAE
                     VGPWKQYLGILLRRTDPSAVAG"
     gene            4884..>5296
                     /locus_tag="VFV59_03440"
     CDS             4884..>5296
                     /locus_tag="VFV59_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324916.1"
                     /translation="MNESQRRIDEPLRRALDAILIRWLGLYVLAGSSAALPLLCQVDH
                     EHMALWRAALPLFLVGFIAVHVLHRHRAPRDDGWERAARADPGTARLTLSVGLAGLFA
                     VGLSLVAIFCPYESPLEAVALLGVWAPILVPLYAVS"
BASE COUNT          774 a         1854 c         1971 g          697 t
ORIGIN      
        1 tcgcagatgg cgtgctcgtt gatgccgcgg cggatgacgg cgttgacctt gatcggtccg
       61 aggcccgcct cctgggcggc ggcgatcccg tccaggatcc gcgccaccgg gatgccggcg
      121 tcgttcatgc gcaggaaggt cgggtcgtcc agggcatcca ggctgaccgt gacgcggtcc
      181 aggccggccg ccgcgagcgc cgccgccttc gaacggagca gcgacccgtt cgtcgtcatg
      241 gtcaggtcgt cgatgcccgg gatctgccgc agcatggcga ccagccgctc gacgtcgcgc
      301 cgcacgagcg gctcgccccc ggtcagccgg accttccgga cgcccagggt gaccgcggcc
      361 gccacgacgc gcgcgatctc ctcgaagtcg aggatctcgg cgcggggcag gaacgcgtga
      421 tcgacgccgt acacctcgcg cggcatgcag taggtgcagc ggaagttgca tcggtccgtc
      481 acgctgaccc gcaggtcgtg gagcggccgg tcgagggcgt cgcgcaccga tgccgcggcc
      541 tgatgggggt tcatgctggc cccatggtgg cagcgcgggg cccgactgcc aaccgggcag
      601 ccgtgtgggc ctagggggcg atggcctgca gcatctggcc gaacgtctcg gccggcagct
      661 gcccggtgac ccgctgcacg acgcgcccat cggcatccac gaagacgaag cccgggaagg
      721 cgctgatgcc cagcgcatcc agcccgcggc tgttgccgtc gtcgaccatc gttggcgccg
      781 tccacccctc gcggtcgagc cacgtggacg gtgggtagtt ctgggcggtc ggcgtgatcg
      841 aggtcgtgag cgtgacgatc ttcacgccct cgggcacctg gttggtggag agccagtcga
      901 ccagttgggg cacctcggcc tggcagacgt ggcaccagtg ggcgaggacg acgatcgcca
      961 tcggcccctg gtccgggccg atggtcatcg gctgcgcgga caggtcggtg ccggtcagcg
     1021 tggggatcgg ctggccgagc gcggtatcgg acgtgttcgg gtcgtaggcg gggagcggat
     1081 tgccgctgat ggcgatgggc gtcgtggccg gctcacgcag cccgccgcca ccgccgctga
     1141 gcagcagggc ggccacggcg gccacgacga cgagcaggag gatgccgccg cccacgagca
     1201 gcaggggcgt gcggttggtg cgcggcgcgg tctcgacggt ggtgcggcgc ggcatgccgc
     1261 gcgatcggtc ggctcgggtg gtcacgagat cggctccgtg ttcgtgatga ggtggtcgcg
     1321 tgtctcctcg gcggcaggcg cgggacgcgc gtacacgagc aggagcgtca cgatggcgag
     1381 gaagcccagc agggccatgg tcgggatcgt cacgaacccg aagcgctcga cccagatgac
     1441 gttgcaggga gactggagcg agcaccacgc cgacggcata ccggggagcc gctcgacgcc
     1501 gatgtggtag atcgagaggg cggcaccgat gagggccacc ggcaccgcgt accacgccac
     1561 ccgcgggtcg cgccgcagcg cggcaacggc caggatcggg gccagcgggt acatggcaat
     1621 gcgctggtac cagcacaggt cgcacggcac gaagccggcg ccgatggaga agtagaggct
     1681 cccggccatc gccacggcgg cgaccaggaa ggcgagccac agcgccgccg gacgaacgag
     1741 ctcggtgaaa ccatccatgg cgccgggaac cagaccgatg cggtccagca cccacagcgt
     1801 gagggtggcg agcacgccga acccggcgcc gagcgccagg agggcgagga aggtggagac
     1861 gacttcgacg ctcatgggac gtcggccgcc gtcgaccgat ggcggatcat gggcccgatt
     1921 ctgccatccg ccgcccagcc ggcaatgggg ccgaacgtcc ccatcgtccg gtgtcaaccg
     1981 gctccgatgg gcccctaagc cgactccccg tggccatgcg gatggtccac caggcgcatc
     2041 cgcaacgcgg gcaacggtcg cggatccgga tcgggctcgt actcgtcggt gttcggattg
     2101 accgcgtagg tccagcgcgg gccatcggtc gcgatggcgc gcagcgcatc ggccagggcg
     2161 tcgacgtcgt cccggctgga gcccagtccc aggctcatgc gtgccgcccc cggcagccgc
     2221 tcgtggtggc cggcgcgggt ctcctccacc agccgatgcg cctcggcgtc gtccaccttg
     2281 agcagccgca tcatcagcgg gtgcgcgcag aagcagccgt ggcggatgcc gatgccgtgc
     2341 tccgccgaca ggaccgcggc cagcaggtcg tacggcacgg atccgaggct gaaggtcatc
     2401 agtccgacgc gtgggtggtc cgtcgaccag acccggtagt gccggaagcc ggggacgtcg
     2461 cgcaggcgct cgcgggcgta ggccagcagg gcgcgttcct cgtccgccac gcgctgcatg
     2521 ccgaccgacg cgagcgtctc gcacgccacc cccaacgcca ccgcgccgac cacgttcggt
     2581 gagccggcct cctggcggtc ggggaggtcc gcccacagcg tctcgtcgac ggtgaccagc
     2641 ttcaccgccc cgccgccgcg caggaagggg tcgccggcac ccagccagtc gcgctcgccg
     2701 atcagcgcac cggcgccgta cggcgcgtac agcttgtgcc cggacagcgc caggtaatcg
     2761 atcccgtcgc gctgcatgtc gatcggcgcg tgcggcgcga gctgggcggc gtcgaccagc
     2821 aggcgcgccc cgttgtcgtg cgcgatccga gcgacctcgg cgatgggcca gatctcgccg
     2881 gtgacgttgc tcgctcccgt gaccgccacc aggtggggat cgccgcaggt cggcagggcg
     2941 cgcagcgtcg cctcgagggt ctcgagcatc tgcgccggcg actcgggaac gggcaggtag
     3001 cgcaccccga tccgcttcca cggcagcagg ttggcgtggt gctcgccggc gaagcagacg
     3061 acgtgcgtcc ctgatggcag cgtgccggcc aggaggttga tggagtccgt ggtgttgcgg
     3121 gtgatgatca ccgcgtcgtc gtcgcgcgcg ttgacgaagc ggcggatcga ctcgcgcgcg
     3181 ccctcgtacg ccgtcgtggc cacgcgcgac ttgaagcccg ccccgcgatg gaccgacgag
     3241 taccagccga ggagctcctc caccgcatcg tggaccgtgc gcagcgcggg ggcggaggcg
     3301 gcgtagtcga ggttgacgta gcgccgcgtc tcgcccgtga cgagcggcac cgcgacctcc
     3361 tcccccacga ggcggacgag ctcgctgccg tggaccggtg ccggttcggt ggccgtcatc
     3421 ggcgccatgc tacccccggt tcacggcagg aagaccagct ttgccgccgc caggacgagg
     3481 acgaacgcca ggacacgccg caacccgagg atggtgaagc ggtgcgcccc caaccacgag
     3541 ccgagcaccc caccggcgac gacggcgagc acccagatgg gcatcgcggc ctgcagcacg
     3601 aagccgatgg tcagcagtcc cgcgatgccc gacagcgagt tgaccaggat gaaggcgccc
     3661 gacagcccgg ccgcgtcgcg cacgccggtc cacctcgtga gcaccagcaa cggggtcagg
     3721 aagatgcccc cgcccgtccc gctcagaccg gccagcagac cgatgccggc gccgctcgcg
     3781 acgccggcca gcggcggaat cgcggggcgc tccggttctc cgtcggtggt cgaggccgtc
     3841 gcgagccgcc aggcgccggc gaggagcacc acggccacca gcggtcggta cacctcgccc
     3901 ggcagctcga tgctgccgcc caggaacgcc atcgggatgg agccgattgc cagcggcacg
     3961 agctggcgcc acggcaggtg gccggcgcgc gcgaagcgca ccaccacgat cgaggccaca
     4021 aagaggttca ggacgagggc cgtcggtcgc atggtcgccg gcgccacccc gaccagtgcc
     4081 atggcggcga ggtacgcgga ggcgccggca tgcccgacac tggcgtacag cagcgccgcc
     4141 ccgaagaaga gcagcgccag gaggagggcg tcgagctcca cgcggcgagt ggctcagccc
     4201 gcgacggcgg agggatcggt gcggcgcagc aggatgccca ggtactgctt ccacggccca
     4261 acctccgcgt cgtgcgagcc ggcgaggccc gcgacgatct ggccgaggtg attgaggtcg
     4321 tggaccgccc acgtggccag caggttgccg agcgtcacct cgccgagcgc gggatgcatg
     4381 ccctgccggt cgaggtcgcc ctcgtgcgcc agcgcctcga agcggccgag gttctcggct
     4441 cgcagcgcgg cgaagcggtc gagcagctcg tcgagcgttg cgtcggcatc gcgctcgaac
     4501 atcgcggtgc ggtccatcgg atcgaacggc acggccgtgc cgtgctccag gatgcgctgg
     4561 gtgcggggca gccagtcgtc gacctcggcg gtgatgaggt gtccgacgac gtcacgcggg
     4621 cgccagccgt ccggcgtatc gggcgtatcg gtccaggcct ccgggagtcc cgccaggagc
     4681 gccgtgagga gctcgggcgt gcggcgcagc agcgccagcg tatcggcgat gcggtcggtc
     4741 atggtcggat gctaggcggt cgggtcgcag gtcgcacgga ctcccgcgcg gggccgatcg
     4801 gcccttgagc caggtggccc cggccacgac gctgaacggc gcccgggagg gacgtgagcg
     4861 cccgcagtgg aggtggtcgc gtcgtgaacg agtctcaacg ccggatcgat gaacccctgc
     4921 gccgcgccct cgacgccatc ctgatccgct ggctcggcct ctacgtcctc gccggctcgt
     4981 ccgcggcact gccgctcctc tgccaggtgg accacgagca catggcgctg tggcgcgcgg
     5041 cgctgcccct gttcctggtc gggttcatcg ccgtccacgt gctgcaccga catcgcgcgc
     5101 cacgcgacga cgggtgggag cgggccgctc gtgccgatcc cggcacggca cgactcaccc
     5161 tgagcgtcgg cctcgcggga ctgttcgccg tgggcctctc gctggtcgcc atcttctgtc
     5221 cgtacgagag cccgctggag gcggtggcgc tgttgggcgt gtgggcgccg atcctcgtcc
     5281 cgctctacgc ggtgtc
//
LOCUS       DASTYP010000067         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1352409, whole genome shotgun sequence.
ACCESSION   DASTYP010000067 DASTYP010000000
VERSION     DASTYP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352409"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFV59_03445"
     CDS             <1..371
                     /locus_tag="VFV59_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5324917.1"
                     /translation="LLASHLVNDPDRGVAFAHTAATHVAPSGIVIGETYPPGWDPEAA
                     VGQTTRLGDASVRLERARVDGDRLTAEVRYGVDGVEWSQPFTARVLDERAIRRLLADA
                     GLRFDRWLNGPGWFAARPAR"
     gene            complement(390..746)
                     /locus_tag="VFV59_03450"
     CDS             complement(390..746)
                     /locus_tag="VFV59_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5324918.1"
                     /translation="MAEYLIYFNQQWVGDHSAEWFRSRGPLANAVLDEMREAGVYVFA
                     GGLDEDGPVSSVDATSGTPVVIDGPYVETKEVLGGFTVVNVPDEATAKAWAVKVAEAC
                     GWPQEVRRFMPQPGSE"
     gene            complement(823..1509)
                     /locus_tag="VFV59_03455"
     CDS             complement(823..1509)
                     /locus_tag="VFV59_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324919.1"
                     /translation="MGQEPTAEAQLADTRLAIDAQRTELERTADQLRDALDLKKRFQE
                     NPALFVGVGAGAVFLLTGGPVRVAKLVRRRLFPSDPEKAYDALPKPMQSWVDHMTSTL
                     GPRADEARQSLAHELLLWRHDPRKHGKVSKKLAKQIAEGPPGPQRAVWQAFEAGAAII
                     TAALARKAVERFLSGEPPSGHPTLDAVGTVGGAAKPDPEHARNDVTNSPSQRATAARQ
                     DYSGISSRDR"
     gene            complement(1476..1883)
                     /locus_tag="VFV59_03460"
     CDS             complement(1476..1883)
                     /locus_tag="VFV59_03460"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5324920.1"
                     /translation="MTTGDAPRRRENPLTLVKRLVSGGVNLAKLEVQRGRQEMARNLG
                     HLRGGIVKLAIAAAIAVVFLIALIAFVMAVLVVIGLWWVALILLVLLVLIAGLLGWRG
                     VKQVSSTNFTPEETIASVKEDIAWAKSRLLKRS"
     gene            2010..2705
                     /locus_tag="VFV59_03465"
     CDS             2010..2705
                     /locus_tag="VFV59_03465"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEU5324921.1"
                     /translation="MAKRRTSGIQRRERASSDALNRRLDDRPSSPLADWRVLAIGGLL
                     VVGAIVVVLVVLFGSGPNPNAGTAQPDDGRDHTSEGTNCRTDPASCGVSGVPYSSIPA
                     TSGPHWNTPANWGVYATPQNESQLLHNLEHGGVVIWYQPDQLDEQQLSELADYVRTQA
                     SSGISGRFKFILSPWSGEDFGHPIAVTSWRNLLYLDSVDLGAIGDFVSAHYGQSPEPN
                     GGPGPGQQTDAMP"
     gene            2748..2822
                     /locus_tag="VFV59_03470"
     tRNA            2748..2822
                     /locus_tag="VFV59_03470"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2780..2782,aa:Lys,seq:ctt)
     gene            2935..4212
                     /locus_tag="VFV59_03475"
     CDS             2935..4212
                     /locus_tag="VFV59_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018737710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5324922.1"
                     /translation="MAHVPIERHALDNGLRIILSRDPRAPLVAVNLWYDVGSKHEKLG
                     KTGFAHLFEHMMFQGSANVEKGQHFGLVQAAGGTLNASTWLDRTNYYETLPGHELELA
                     LWLEADRMASLLPAMTQEKLDNQRDVVKNERRYSVDNQPYGDWDERVQALVYPEAHPY
                     HHPTIGSMEDLSAASLDDVGEFFATYYAPNNAVLTIAGDFERDEALRMVERHFGPIPA
                     NPALPAPPDMTVEPLIGREVRETIPDRVPLPRLYVAARVPPFGTDGFDDMEVVSDLLG
                     TGRASRLYASLVREQRVAQDVTVFVFPIVGGAAMLTLWATAREGVTPETLEAALFTEL
                     SRLAQDGPRDEELERVRNLHAAHAAGSLERIGERADRLSMYTCLFDEPDRINHEVERY
                     DAVDADRVRASMQAFLVPDNRVLLTYVPAEAKA"
BASE COUNT          647 a         1519 c         1439 g          617 t
ORIGIN      
        1 tgctgctggc cagccatctg gtcaacgacc ctgaccgtgg cgtcgccttc gcgcacacgg
       61 cagcgacgca cgtcgccccg agcggcatcg tgatcggcga gacctacccg cccggctggg
      121 atccggaagc ggcggtcggc cagacgaccc ggctcggtga cgcaagcgtc cgactcgagc
      181 gagctcgcgt ggacggcgac cggctcaccg ccgaggtccg gtacggcgtc gacggcgtgg
      241 agtggtcgca gccgttcact gcccgcgtgc tggacgagcg cgcgatccga aggctgctcg
      301 ccgacgccgg cctccggttc gatcgctggc tcaacggacc cggctggttc gcggcgcggc
      361 cagcgcgcta gaagcctggc tctccccgtc tactccgatc cgggctgcgg catgaagcgg
      421 cgaacctcct gcggccagcc gcacgcctcc gcgaccttga cggcccacgc cttggccgtc
      481 gcctcgtcgg gcacgttcac caccgtgaag ccgccgagga cctccttggt ctcgacgtag
      541 ggtccatcga tcacgaccgg cgtgccggac gtcgcgtcca cgctggagac gggcccgtcc
      601 tcgtccagcc ctccggcaaa gacgtacacc ccggcctcgc gcatctcgtc gaggaccgcg
      661 ttggccagcg gcccgcgcga gcggaaccac tcggccgagt ggtcgccgac ccactgctgg
      721 ttgaagtaga tgaggtactc ggccacggct cctcctgggt gcggcaggcc agcggccgcc
      781 ttcaccagta cgacgaacgg cgacgagcgc cttcgacggc cgtcagcgat cgcgggagct
      841 gatccccgag tagtcctggc gcgcggcggt ggcccgctgg gacggcgagt tggtgacgtc
      901 gttcctggcg tgctcgggat cgggcttggc cgcacccccg accgtcccga cggcgtcgag
      961 ggtcggatgg cccgacggcg gctcgccgga gaggaaccgc tcgacggcct tgcgcgcgag
     1021 cgcggcagtg atgatcgccg cgcccgcctc gaaggcctgc cagaccgcac gctgcggccc
     1081 aggcgggccc tcggcgatct gcttcgccag cttcttgctc accttgccgt gcttgcgcgg
     1141 gtcgtgccgc cacagcagca gctcgtgggc gagcgactgg cgtgcctcgt cggcgcgcgg
     1201 gccgagggtg gacgtcatgt ggtcgaccca ggactgcatc ggcttgggga gggcgtcgta
     1261 ggccttctcc ggatcggagg ggaagaggcg gcgccggacg agcttggcaa cccggaccgg
     1321 gcccccggtc agcaggaaga cggcaccggc tcccacgccg acgaacagtg ccgggttctc
     1381 ctggaaccgc ttcttcaggt cgagcgcgtc gcggagctgg tccgccgtcc gctcgagctc
     1441 ggtgcgctgg gcgtcgatgg ccagccgcgt atcggctagc tgcgcttcag cagtcggctc
     1501 ttggcccacg cgatgtcctc cttcaccgat gcgatcgtct cctccggggt gaagttcgtg
     1561 ctcgagacct gcttcacccc gcgccagccg agcagcccgg cgatcaacac cagcagcacg
     1621 agcaggatca gggcgaccca ccacaggccg atgacgacga ggaccgccat cacgaacgcg
     1681 atgagcgcga tgaggaagac gacggcgatg gccgccgcga tcgcgagctt cacgatgccg
     1741 ccacgcaggt ggccgaggtt gcgggccatc tcctgccggc cgcgctggac ctcgagcttc
     1801 gccaggttca ccccacccga cacgagccgc ttcacgagcg tgagcggatt ctcgcgacgg
     1861 cgtggcgcgt cgcctgtggt catgtcacct ccggggagcg ggcgaatcaa acggcgcggc
     1921 acagcgacgg cgcagcaccc tcgattctag caacgagcag gttgggtgcc agcgcaccga
     1981 tccggctctc ccgctatcct tccgcgccaa tggccaagcg acgaacttcc ggcatccagc
     2041 gacgcgagcg cgcctccagc gacgcgctga accgacgcct cgacgaccga ccctcctccc
     2101 cgctcgccga ttggcgagtt ctcgcgatcg gtggactgct ggtcgtgggg gcgatcgtgg
     2161 tggtgctcgt ggtcctgttc ggctccggcc cgaacccgaa cgcggggacg gcccagccgg
     2221 acgatggtcg ggatcacacc agcgagggca cgaactgccg aaccgacccc gcgtcgtgcg
     2281 gcgtgagcgg cgttccgtac tcctcgatcc cggcaacgtc gggcccccac tggaacacgc
     2341 cagccaactg gggtgtgtac gccacgccgc agaacgagtc gcagctgctg cacaacctcg
     2401 agcacggcgg cgtcgtgatc tggtaccagc ccgaccagct cgacgagcag cagctgagcg
     2461 aactggccga ctacgtccgg acccaggcct cgagcggcat cagcggtcgg ttcaaattca
     2521 tcctctcccc gtggagcggc gaggacttcg gccaccccat cgcggtcacg tcgtggcgca
     2581 acctgctgta cctcgactcg gtggacctcg gcgccatcgg cgacttcgtg agcgcccact
     2641 acggccagtc acccgagccg aacggcggtc cgggacccgg gcagcagacg gacgcgatgc
     2701 cctgacgttc ggttgaccgg cactacggcg tacggcatca tttccgcggg cgcgtagctc
     2761 aacggcagag caaaggactc ttaatcctta ggttgtgggt tcgaatccct ccgcgctcgc
     2821 caaccacgac ctgatccgat gaacgggccg cccaccgcgg cccgttcggt cttctcggct
     2881 tcggcttctc cggccgcgca gcggccgcat cggtcccgca cagcggaggt gctggtggct
     2941 cacgtaccga tcgaacggca cgccctcgac aacggcctgc gcatcatcct gtcgcgcgat
     3001 ccccgcgcgc cgctggtggc ggtcaacctg tggtacgacg tcggcagcaa gcacgagaag
     3061 ctcggcaaga ccggattcgc gcacctgttc gaacacatga tgttccaggg ttccgcgaac
     3121 gtggagaagg gccagcactt cggcctggtg caggccgccg gcgggacgct caacgcgagc
     3181 acctggctgg accgcaccaa ctactacgag acgctgcccg gccacgagtt ggagctggcg
     3241 ctgtggctgg aggcggaccg gatggcctcg ctcctgccgg ccatgacgca ggagaagctc
     3301 gacaaccagc gcgacgtcgt gaagaacgag cgccgctaca gcgtcgacaa ccagccctac
     3361 ggcgactggg acgagcgagt gcaggcgctg gtctaccccg aggcgcatcc gtaccaccac
     3421 ccgaccatcg gctcgatgga ggacctgtcg gccgcctcgc tggacgacgt gggcgagttc
     3481 ttcgccacct actacgcgcc gaacaacgcg gtgctgacca tcgccgggga cttcgagcgt
     3541 gacgaggcgc tgcgcatggt cgagcgccac ttcggaccga tcccggccaa cccggcgctg
     3601 ccagcgccgc ccgacatgac ggtggagccg ttgatcggcc gcgaggtgcg cgagacgatc
     3661 ccggaccgcg tgccgctccc ccgcctctac gtggccgccc gcgtcccgcc cttcgggacc
     3721 gatggcttcg acgacatgga ggtggtcagc gacctgctcg gcaccgggcg cgcctcgcgg
     3781 ctgtacgcgt cgctcgtccg tgagcagcgc gttgcgcagg acgtgaccgt cttcgtcttc
     3841 cccatcgtcg gcggcgcggc gatgctcacg ctgtgggcca ccgcccgtga gggcgtgacg
     3901 cccgagacgc tggaggccgc cctgttcacc gagctgtcgc ggctggcgca ggatgggccg
     3961 cgagacgagg agctggagcg cgtccgcaac ctgcacgcgg cgcacgcggc ggggtcgctg
     4021 gagcggatcg gcgagcgggc ggatcggctg agcatgtaca cctgcctttt cgacgagccc
     4081 gaccgcatca accacgaggt cgagcgctac gacgcggtcg acgccgatcg ggtgcgcgcg
     4141 tccatgcagg ccttcctggt gcccgacaac cgggtgctcc tcacctacgt tcccgcggag
     4201 gcgaaggcat gacgctccga cc
//
LOCUS       DASTYP010000068        13345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1425566, whole genome shotgun sequence.
ACCESSION   DASTYP010000068 DASTYP010000000
VERSION     DASTYP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13345
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425566"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /gene="glgB"
                     /locus_tag="VFV59_03480"
     CDS             complement(<1..2159)
                     /gene="glgB"
                     /locus_tag="VFV59_03480"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU5324923.1"
                     /translation="MTTSDLAPAATDIERLARGEHHDPHAVLGAHPTGGGTVVRAFHP
                     DATDAALVAPNGAAHPMQSVGGGLWAAHVDDLAPHQVSYRVRFTFSDGNTWEREDPYR
                     FAPTVGEVDLHLIGEGTHERLYDVLGAHPRTHQGVSGVSFAVWAPNARSVRIVGDFDR
                     WDGRLLPMRSMGASGVWELFVPGIGPGELYKYEILGADGKLRLKADPLAFAMQVRPET
                     ASRVWDPSVYPWTDQAWMAERAQRDPYRSPMAAYEVHLGSWMRNPDGSWLGYRQAAEK
                     LVEHCHRFGFTHVELLPVAEHPFDGSWGYQVTGYYAPTARFGNPDDFAALVETLHNAG
                     IGVIVDWVPAHFPNDDFALRRFDGTPLYEHADPRRGEHPDWGTLIFDFGRPEVRNFLV
                     ANALFWCDRYHIDGLRVDAVASMLYLDYSRKAGEWTPNVHGGRENLDAIVFLREMNER
                     VYGRFPGVVTIAEESTAFPGITRPTYLGGIGFGFKWDMGWMHDTLKYFSEDPVHRRYH
                     HDKLTFRGLYKDTEHFVLPLSHDEVVHGKGSLQRKMPGDPWQRFANLRALLVDMWGQP
                     GKKLLFMGAELGMWEEWSHDRPLPWELLDAPMHGGMARFVEDLGRVYRSTPALWASDD
                     APDGFEWIDASNVEESVYAWIRRGGEGRGSEAVVVLNLTPVPRHGYRLGMPSAGRWVE
                     AINSDSEHYGGSNLGNLGGVEAVEERFHGQPASASLTL"
     gene            complement(2223..3080)
                     /locus_tag="VFV59_03485"
     CDS             complement(2223..3080)
                     /locus_tag="VFV59_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324924.1"
                     /translation="MTRSSDELEVTVLPGLGCRVHRLRAFGRDLVRTPPEPATHADDP
                     FFWGAYVMAPWTNRASAEPMTVAGRRVDLAANFPDGTAIHGQVYAAPWRRTGDATFGV
                     DHAADAAWPWDYTVTAELAVDGPRLRLRYRLENRSDATMPGGLGLHAWVRRPVALALR
                     ARAVDPANGRPDAAEVTATARWGLDDTAPPDGVDATWLDLEPTEIGLRWPAEGLGATL
                     RAASSGTAVHAAVANPEEPAATAVEPVTHRPWGLDRLARGETGAMDVLAPGTSLALDL
                     ELAVHRAAP"
     gene            complement(3085..4758)
                     /locus_tag="VFV59_03490"
     CDS             complement(3085..4758)
                     /locus_tag="VFV59_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU5324925.1"
                     /translation="MTVRRLALVAAVPVALAGAVGAWVLANGRGSTPSEALEAPRFVD
                     EAGPSGVDHTYDGDSPFFVGGGVATLDCDGDAFPDLYLAGGANPAALYRNDSEVGGTL
                     RFEHLTDAVTDLTGVTGAYPLDLDGDGLTDLAVLRVGENVLLRGLGDCRFERANERWG
                     FDGGEARTMAFAATWEGHATLPTLAIGDYIPLTEHGTPVPPCPDNRLLRPTADGTTYG
                     EPIPLTPGYCPLSMLFSDWDRSGRRDLRVSNDRQYYTDGEEQLWRIEPGADPVAYTAA
                     DGWTRLQVWGMGIASQDLTGDGYPEVYLTSQGDNKLQTLMAGPDAPTYRDIAYAAGVH
                     APRPFTGGDAGGSTAWHPEFVDVNADGFLDLYVTKGNVSQQVGYAMRDPSELFLGQPD
                     GTFRQATEDAGLLRFDRGRGATLADLNLDGLPDLVEVYLSEPVGVWRNVGGGTTDAPQ
                     PMGHWLELRLSQGGRNRDAVGAWVEVRIGEATILHEVTVGGGHISGDAGWIHVGLGPS
                     ERAEVRVTWPDGTVGPWQQVAADDLVIVARDADQPRVVTPGREDSSGDG"
     gene            complement(4769..6634)
                     /locus_tag="VFV59_03495"
     CDS             complement(4769..6634)
                     /locus_tag="VFV59_03495"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEU5324926.1"
                     /translation="MPSTRSRWATVATVLAVGVLVVGGIVAVMRPWGEYRACAPDAEH
                     PEWSVARRWDEALLDAIRRALPAPTVHGRNLFHTSAAMWDAWAAYDPVARGVFVTEKH
                     TAADVAAARDEAISYAAYRLLTSRYINAVGGNESLSEFADLMDSLCYPLDVTTTEGDT
                     PAAFGNRVATTILAAVAEDGSNEAGGYAPADYEPVNPPLVVAGDEISMVDPNHWQPLQ
                     IEHMISQNGIPVTNGVQQAIGPHWGHVTSFAIPPGGTEGVPMDPGPPPQFGSDEYRDQ
                     AVQVIRLSSELDPSDPTTMDVSPGAIGDNPLGTNDGDGYEVNPVTGQPYPPDVVLRAD
                     FGRVMAEFWADGPHSETPPGHWNVITNLVGDELGTDLRIGGEGPTVDRLEWDVKAYLA
                     VNGAVHDAAIAAWGLKGHYDGVRPISMIRYMGSLGQSSDPDGPSYDPNGLPLVDGLIE
                     VITDGTTKPGGRHAALAGHEGEIAIRSWAGNPEDPETQVGGVAWIRAVEWIPYQLPTF
                     VTPAFPGYVSGHSTFSRAAAEVLTRLTGSAYFPGGESGYTIPAGSLAFEMGPTTDIRL
                     EWATYYDAADQAGQSRLWGGIHISADDLNGRRTGAACGIAAWSRASAYFAGVDGS"
     gene            complement(6649..7635)
                     /locus_tag="VFV59_03500"
     CDS             complement(6649..7635)
                     /locus_tag="VFV59_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5324927.1"
                     /translation="MSVLRWGVLSTAAIGRKVIPGIQGADNCEVVAIASRDPERGRRV
                     AEERGIPTVHASYEALLADPTVDAVYIPLPNHLHAEWAMAAARAGKHVLCEKPLALTA
                     ADAERMIEAADAAGVRLMEAFMYRHHPSWVAVRELVAGGRIGQLVAVQTWFSFFNDDP
                     ANIRNIVETGGGALYDIGCYAVNLSRMLFAAEPMRVTSAIRRDPLGGTDTLASAILEF
                     DAGIASFTVSTRAEDDQRVHVHGTTGRISVEIPFNIPPDRPTRIHLASGGEPPVAPNV
                     ETLTFPAADPYTAEASAFATAVLEDRPVPVPPADAVANLRVIETIFRAAEGS"
     gene            complement(7639..8346)
                     /locus_tag="VFV59_03505"
     CDS             complement(7639..8346)
                     /locus_tag="VFV59_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5324928.1"
                     /translation="MDVLHGRVAMITGASRGIGAATARSLHGAGMRLGLVSRSGDGLG
                     LDDAIGIRGDVRDGASMRDAANAVAERFGGIDVLVVNAGVGAYGPILDLPEEHLEEMV
                     EVNVKGAIHSVRAALPHLLRSQAADIVTVASEAGRRGLPNEAVYCATKFAQVGLTAAL
                     DHELREHGIRCTNVCPGGVATDFAMGRGRTPDMPALAGMMRPEDVAEAVLWVLTRPRT
                     HRILEVAFRPVTEPSWG"
     gene            8486..9715
                     /locus_tag="VFV59_03510"
     CDS             8486..9715
                     /locus_tag="VFV59_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5324929.1"
                     /translation="MSVPGDLVGQRSETVRRSNLSAIVRELHVRGPLSRSDLVGLTGL
                     TRSAIRGLIGELVAGGLVTEVPNQPAGTPGRPSPIVQPNARGAVAVALEVNVDSLAAA
                     LVGFGGTLHQLARVDRPRGHLSVDAIVDDLVELVGPLLRAVDPDALVGIGVAVAGIVR
                     RSDGIVRMAPNLGWRDVPLAQRLREALPVVAPVWVANEADLGGLAEHRRGAAIGADEV
                     LYVMGEVGVGGNMIVDGRPIIGIDGYAGEIGHMSVNPDGLACACGSRGCWETEIGERA
                     LLTRAGFDPEGGREAIGEVIAAATAGEPRAVTAVEEVGRWLGIGISSLVNVLNPQLVV
                     VGGVLERLYPLMARTLEEAVAVYALPPGREHLRIVPGALGDDAPTLGAAELAFEPLLA
                     DAALQLRLRSPAALVSA"
     gene            9871..11064
                     /locus_tag="VFV59_03515"
     CDS             9871..11064
                     /locus_tag="VFV59_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009571730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5324930.1"
                     /translation="MIRRLAALLAVLAVVLAACGGTGTASGSASEPASSGGGGGTTDC
                     TVAVSWNNFQQPRWAAADEPNIKQVVEDAGGTYISADADLSSEQQLTDVSNLIGQGAD
                     VLILLAQDQNAVLPALQEAKDAGIPVIAYDRLIEDPDILYITFNNVGVGEAQAAAVLE
                     AVPEGNYVLIKGDPGDPNASTFLPQGWDDAGLKEKVDAGDITILNGPEGGSGEWPADY
                     GTYTDAWDTQTAQENMEAIIDAANADDVTIDAILAENDSTALGVVAALEAKSYGYPPL
                     SGQDGDPANLNNVALGKQYVDVWKNANELGKAAGQAAIQMCQGTAMADVSIDIESSVA
                     PTDGNSPTDFETPGGNTVKSLILQPTPLTAEDLDVVLSAGWYAGKDEICAGVTESDPG
                     WDDCQ"
     gene            11161..12486
                     /locus_tag="VFV59_03520"
     CDS             11161..12486
                     /locus_tag="VFV59_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5324931.1"
                     /translation="MANVADTAPVPGESRTASTATRMREMLSATEVDVRLFGMLVALA
                     AILIGFQLLDSGFFSPGNMVTLAVQAIPVAILATGMVLVIVSRNIDLSVGSLTGIIAM
                     TYAMLMTDWLPNILGLGVDFPFRWLIALVIGIAVGATFGAVQGFIIAYIGVPSFVVTL
                     GGLLSIRGAIWYFSNGAAVTRLDPTFQLIGGGARGSIGELLTWGIGLLGCAFVIFALW
                     NGRRQRRRFGFPLRPMWAEVTIGVLGCAAMLGLAAFANGNYLPAGLATQYAQERGIVE
                     PPGGLKVPAGFPLPVILLIAVTVVMTWIATRRRFGRYVYAYGGNPDAAELAGINTRRT
                     ILLTFVIMGILAALSAAVVAARLNGATLDIGNSYELYVIAAAVVGGTSFAGGIGTIPG
                     AVLGAFVMQALSYGLSFIGVNSPGQNVVAGIVLILAVAFDTWNRRRAAR"
     gene            12557..13309
                     /locus_tag="VFV59_03525"
     CDS             12557..13309
                     /locus_tag="VFV59_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5324932.1"
                     /translation="MRDIRVSFGGVHAVAGVNIDLHEGEIVGLVGGNGAGKSTLMKVL
                     SGALVADSGEVFVCGERAHLANPRDAKAYGIETIYQTLALADNLDAPANVFLGRELTM
                     GVGSLDDSAMENATRGVMRRLNPNFQNFKTPVVSLSGGQRQSVAIARAVQFNAKILIM
                     DEPTAALGPAETTQVRNLIRQLKEEGIGIFLISHDIHDVYDLSDRISVMYHGRIVDTV
                     RKEDVDTDAVLGMIILGKRPGDVTQEELASLR"
BASE COUNT         1861 a         4812 c         4736 g         1936 t
ORIGIN      
        1 agggtcagcg acgcggaggc cggctgcccg tggaaccgct cctcgaccgc ctccacgccg
       61 cccaggttcc cgaggttcga cccgccgtag tgctcggagt cggagttgat cgcctcgacc
      121 cagcggccgg ccgacggcat gcccaggcgg tacccgtggc ggggcacggg cgtcaggttc
      181 agcacgacca ccgcctccga cccacggccc tcaccgcccc gccggatcca ggcgtacacc
      241 gactcctcga cgttcgaggc gtcgatccac tcgaacccat ccggtgcgtc atccgaggcc
      301 cagagtgccg gcgtggaacg gtagacccgg cccaggtcct ccacgaagcg ggccatcccg
      361 ccgtgcatcg gcgcatccaa cagctcccag ggcagcggcc ggtcgtgcga ccactcctcc
      421 cacatcccca gctcggcgcc catgaacagc agcttcttgc ccggctgtcc ccacatgtcg
      481 accagcaggg cgcggaggtt cgcgaagcgc tgccacgggt cgccgggcat cttgcgctgc
      541 agtgagccct tgccgtggac cacctcgtcg tgggagaggg gcaggacgaa gtgctccgtg
      601 tccttgtaca ggccacggaa cgtcagcttg tcgtggtggt agcggcggtg caccgggtcc
      661 tcgctgaagt acttcagcgt gtcgtgcatc cagcccatgt cccacttgaa gccgaagccg
      721 atgccgccca ggtacgtcgg ccgggtgatg cccgggaagg cggtcgactc ctccgcgatg
      781 gtcacgacgc cggggaagcg gccgtagacc cgctcgttca tctcccgcag gaagacgatc
      841 gcgtcgaggt tctcgcggcc gccgtggacg ttcggcgtcc actcgccggc cttgcgtgag
      901 tagtcgaggt acagcatcga ggccacggca tccacccgca gcccgtcgat gtggtagcgg
      961 tcgcaccaga acagcgcgtt ggccaccaga aagttccgga cctcggggcg cccgaagtcg
     1021 aagatcagcg tgccccagtc ggggtgctcg ccgcgccgcg gatcggcgtg ctcgtacagg
     1081 ggcgtgccgt cgaagcggcg gagggcgaag tcgtcgttgg ggaagtgggc cggcacccag
     1141 tcgacgatca ccccgatccc cgcgttgtgg agcgtctcga ccagcgcggc gaagtcgtcc
     1201 gggttgccga agcgggccgt cggcgcgtag tagccggtga cctggtagcc ccacgatccg
     1261 tcgaacgggt gctcggcgac cggcagcagc tcgacgtgag tgaagccgaa gcgatggcag
     1321 tgctcgacga gcttctcggc cgcctgccgg tagcccagcc acgacccgtc ggggttgcgc
     1381 atccaggagc ccaggtgaac ctcgtaggcc gccatcggag accgatacgg atcgcgctgc
     1441 gcgcgctccg ccatccatgc ctgatcggtc cacggataga ccgatggatc ccagacgcgt
     1501 gacgcggtct ccgggcgcac ctgcatggcg aaggcgaggg gatcggcctt gaggcgcagc
     1561 ttgccgtccg cgcccaggat ctcgtacttg tacagctcgc cgggaccgat gccggggacg
     1621 aagagctccc acacgcccga ggcccccatc gagcgcatcg gcagcaggcg gccatcccag
     1681 cggtcgaagt cgccgacgat gcggacactg cgcgcattcg gagcccagac cgcgaagctc
     1741 acgccgctga cgccctggtg cgtgcgcggg tgcgcgccga gcacgtcgta cagccgctcg
     1801 tgcgtcccct cgccgatcag gtggaggtcc acctcgccga cggtcggcgc gaaccgatac
     1861 ggatcctcgc gctcccaagt gttgccgtcg ctgaacgtga agcggacgcg gtagctgacc
     1921 tggtgcgggg ccaggtcgtc gacgtgggcc gcccacagcc cgccgccgac cgactgcatc
     1981 gggtgggcgg cgccattcgg cgccaccagc gccgcgtccg tcgcatccgg gtggaacgcg
     2041 cggacgactg tcccgccgcc ggtggggtgg gcgcccagga cggcgtgggg gtcgtggtgc
     2101 tcgccgcgcg ccaggcgctc gatgtccgtc gcggctgggg cgaggtcgga ggtcgtcatc
     2161 ggcctaacca tacgctgccc cccggtccgc aagatgcgtg cgagcccctg tctcgtcgtg
     2221 gctcagggtg ccgcgcggtg cacggcgagc tcgaggtcga gcgccaggct cgtgcccggc
     2281 gcgagcacgt ccatggcgcc cgtctcgccg cgcgccagcc ggtccaggcc ccacggccga
     2341 tgggtgacgg gctccaccgc cgtcgccgcc ggctcctcgg gattcgcgac ggccgcgtgc
     2401 acggccgttc cgctgctcgc cgcgcgcagc gtcgcgccaa gcccttccgc cggccagcgc
     2461 aacccgatct cggtcggctc gaggtccagc caggtcgcgt caacgccgtc cgggggcgcc
     2521 gtgtcgtcga ggccccagcg ggcggtggcg gtgacctccg cggcgtccgg gcgcccgttc
     2581 gccggatcca cggcgcgagc ccgcagtgcc agcgccaccg gccggcgcac ccaggcgtgg
     2641 agtcccagcc cgccgggcat cgtcgcatcc gaccggttct ccaggcgata ccggaggcga
     2701 aggcgtggac cgtccaccgc gagctcggcc gtgacggtgt agtcccatgg ccacgcggca
     2761 tccgcggcgt ggtcgacgcc gaaggtcgcg tcgccggttc gccgccaggg cgccgcgtag
     2821 acctggccgt ggatggcggt cccgtccggg aagttggccg ccaggtccac ccggcggccc
     2881 gccacggtca tcggctcggc agatgcccgg ttggtccagg gcgccatgac gtaggcgccc
     2941 cagaagaacg gatcgtcggc gtgcgtcgcc ggctccggtg gcgttcgcac caggtcgcgc
     3001 ccgaaggcgc gcagccgatg cacgcggcag ccgagccccg ggagcaccgt cacctcgagc
     3061 tcgtcgctgg accgggtcac ctgctcaacc gtcgccactg ctgtcctccc tgccgggcgt
     3121 caccacgcgc ggctggtccg cgtcgcgcgc cacgatcacc aggtcgtcgg ccgccacctg
     3181 ctgccatggc ccgaccgtcc cgtctggcca cgtcacgcgg acctcggcac gctccgaggg
     3241 acccagcccg acgtggatcc acccggcgtc gccgctgatg tggcccccgc cgacggtcac
     3301 ctcgtgcagg atcgtcgcct caccgatgcg gacctcgacc cacgcgccga ccgcgtcgcg
     3361 gttccggcct ccctgggaga gtcggagctc gagccagtgg cccatcggct gcggggcgtc
     3421 ggtcgtgcca ccaccgacgt tgcgccacac gccgaccggt tcggacaggt acacctcgac
     3481 caggtcgggc agcccgtcga ggttcaggtc cgccagcgtg gcgccacggc cccggtcgaa
     3541 gcgcagcagg ccggcatcct ccgtggcctg gcggaaggtg ccgtccggct ggcccaggaa
     3601 cagctcgctc gggtcgcgca tggcgtagcc gacctgctgg ctgacgttgc ccttcgtcac
     3661 gtacaggtcc aggaagccgt cggcgttgac gtccacgaac tccgggtgcc acgccgtcga
     3721 tccgccggcg tccccgccgg tgaacggccg gggcgcatgg acgccggcgg cgtaggcgat
     3781 gtcgcggtag gtcggcgcgt cggggccggc catcagcgtc tggagcttgt tgtcgccctg
     3841 gctggtcagg tacacctcgg ggtagccgtc cccggtcagg tcctggctgg cgatgcccat
     3901 gccccagacc tggagccggg tccatccgtc cgccgccgtg taggcgaccg gatcggcccc
     3961 cggctcgatc cgccacagct gctcctcgcc gtcggtgtag tactgccggt cgttgctcac
     4021 ccgcagatcc cgccgccccg accgatccca gtcgctgaag agcatcgaga gcgggcagta
     4081 gccgggcgtg agggggatcg gctcgccgta ggtcgtgccg tcggcggtcg gtcgcagcag
     4141 ccggttgtcg gggcagggcg gcaccggcgt gccgtgctcg gtcagcggga tgtagtcgcc
     4201 gatggccagc gtggggagcg tcgcgtggcc ctcccaggtg gcggcgaagg ccatcgtccg
     4261 cgcctcgccg ccatcgaagc cccagcgctc gttcgcccgc tcgaagcggc agtcccccag
     4321 cccgcgcagc agcacgttct cgccgacccg gagcacggca aggtcggtga ggccatcgcc
     4381 gtcgaggtcg agcgggtacg ccccggtgac gccggtcagg tcggtcacgg catcggtcag
     4441 gtgctcgaag cgcagcgtgc cgcctacctc actgtcgttg cggtacagcg ccgcggggtt
     4501 ggcgccgccg gccaggtaca ggtccgggaa cgcgtcgccg tcacagtcga gggtggcgac
     4561 gccgccgccc acgaagaacg gcgagtcgcc gtcgtaggtg tggtcgaccc cgctcgggcc
     4621 ggcctcgtcg acgaagcgtg gagcctccag cgcctcggag ggcgtggagc cgcggccgtt
     4681 cgcgagcacc cacgctccga cggccccggc cagcgccacc ggcaccgccg ccaccagcgc
     4741 gagacgccga acggtcaacc gatgcgcctc aggagccgtc gacgccggcg aagtacgccg
     4801 aagcccgcga ccacgccgcg atcccgcatg ccgccccggt ccgccggccg ttgaggtcgt
     4861 ccgcgctgat gtggatgccg ccccacagcc gcgactgccc ggcctggtcg gcggcgtcgt
     4921 agtaggtcgc ccattcgagg cggatgtcgg tggtgggccc catctcgaag gccagcgagc
     4981 ccgccgggat ggtgtagccg ctctccccgc cggggaagta ggcgctgccg gtcaggcgtg
     5041 tcaggacctc cgccgcggcg cggctgaagg tgctgtggcc ggagacgtag cccgggaacg
     5101 cgggcgtgac gaaggtcggc agctggtacg ggatccactc gaccgcccgg atccaggcga
     5161 cgccgccgac ctgtgtctcc gggtcctccg ggttccccgc ccacgagcgg atggcgatct
     5221 ccccctcgtg gccggccagc gccgcgtggc gaccgccggg cttcgtcgtc ccgtcggtga
     5281 tgacctcgat caggccatcg accagcggca gcccgtttgg atcgtacgag gggccgtccg
     5341 ggtccgacga ctggcccagg ctgcccatgt agcggatcat ggagatcggt cggacgccgt
     5401 cgtagtggcc tttgaggccc catgcggcga tcgccgcgtc gtgcacggcg ccattcacgg
     5461 cgaggtacgc cttcacgtcc cactcgaggc ggtcgacggt cgggccctcg ccaccgatgc
     5521 gcaggtccgt ccccagctcg tcacccacca ggttggtgat gacgttccag tgcccaggcg
     5581 gcgtctcgga gtgcggcccg tcggcccaga actccgccat gaccctgccg aagtcggcgc
     5641 gcagcacgac gtccggcggg tacggctgtc cggtgaccgg gttgacctcg tagccgtcgc
     5701 cgtcgttggt gcccagcggg ttgtcgccga tggcgcccgg tgacacgtcc atggtggtgg
     5761 ggtcggacgg gtccagctcg ctcgacagcc ggatcacctg gacggcctga tcacggtact
     5821 cgtcgctgcc gaactgcgga ggcgggccgg gatccatcgg cacgccctcg gtgccgccgg
     5881 gagggatcgc gaacgacgtc acgtggcccc agtgtgggcc gatggcctgc tgcaccccgt
     5941 tggtcaccgg gatgccgttc tggctgatca tgtgctcgat ctggagcggc tgccagtggt
     6001 tgggatcaac catgctgatc tcgtccccgg cgaccacgag cggtgggttg accggctcgt
     6061 agtcggccgg cgcgtagcca cccgcctcgt tcgagccgtc ctcggcgacg gccgccagga
     6121 tggtggtggc aacgcggttg ccgaacgccg ccggcgtgtc gccctcggtc gtcgtcacgt
     6181 ccagcggata gcacagcgag tccatcaggt cggcgaactc ggagagcgac tcgttgccgc
     6241 cgaccgcgtt gatgtagcgc gacgtcagga gccggtaggc ggcgtagctg atcgcctcgt
     6301 cgcgggccgc ggcgacgtcc gcggcggtgt gcttctcggt cacgaacacg ccccgggcga
     6361 ccgggtcgta ggccgcccag gcgtcccaca tcgcggccga ggtgtggaac aggttgcgcc
     6421 catggaccgt cggcgccggc agcgcccggc ggatggcatc gagcagggcc tcgtcccagc
     6481 gccgcgccac gctccactcg gggtgctccg cgtcgggcgc acaggcgcgg tactcgcccc
     6541 atggccgcat gacggcgacg atgccgccga cgaccaggac gccaacggcc aggacggtcg
     6601 cgaccgtcgc ccaccgcgag cgggtgctcg gcatggctcg gctcctcctc agctgccctc
     6661 cgcagccctg aagattgtct cgatcacgcg caggttggca acggcatcgg ccggcggcac
     6721 gggcaccggc cgatcctcga gcacggcggt ggcgaaggcg gacgcctcgg cggtgtacgg
     6781 atcggccgcg ggaaaggtca gcgtctcgac gttcggcgcc accggcggct ccccgcccga
     6841 ggccaggtgg atgcgagtcg gccggtccgg cgggatgttg aacgggatct cgacgctgat
     6901 ccgcccggtc gtgccatgga cgtggacgcg ctgatcgtcc tcggcccgcg tcgacaccgt
     6961 gaagctggcg atgccggcgt cgaactccag gatggcgctc gcgagcgtat cggtcccgcc
     7021 cagcgggtcg cgtcggatgg cgctggtgac ccgcatcggc tcagccgcga acagcatgcg
     7081 cgacaggttc accgcgtagc agccgatgtc gtacagcgca ccgcccccgg tctcgacgat
     7141 gttgcggatg ttggccggat catcgttgaa gaagctgaac caggtctgga ccgccacaag
     7201 ctgaccgatg cgcccgccgg ccaccagctc gcgcacggcg acccacgacg ggtggtggcg
     7261 gtacatgaag gcctccatca accgcacgcc ggccgcatcg gccgcctcga tcatgcgctc
     7321 ggcgtcggcc gccgtcaggg ccagcggctt ctcgcacagc acgtgcttgc cggcgcgcgc
     7381 cgcggccatg gcccactcgg cgtggaggtg gttggggagc gggatgtaga cggcgtcgac
     7441 ggtcgggtcc gccagcaggg cctcgtacga ggcgtgcacg gtcgggatgc cgcgctcctc
     7501 cgcgacccgg cgcccccgct ccgggtcccg ggaggcgatg gccacgacct cgcagttgtc
     7561 ggcgccctgg atcccgggaa tcaccttgcg gccgatggcc gcagtggaca ggacgcccca
     7621 gcgcagcacg ctcaccgctc agccccagga cggctcggtg accgggcgga aggcgacctc
     7681 caggatccga tgcgtccgcg gccgggtcag cacccacagc acggcctcgg ccacgtcctc
     7741 gggtcgcatc atgccggcga gggccggcat gtccggcgtt cgtccgcgtc ccatggcgaa
     7801 gtcggtggcc acgccgccgg ggcacacgtt ggtgcagcga atgccgtgct cgcgcagctc
     7861 gtggtcgagc gccgcggtca acccgacctg ggcgaacttc gtggcgcagt acaccgcctc
     7921 gttgggcagg ccgcgccgcc cggcctcgct cgccacggtg acgatgtcgg cggcctggct
     7981 gcgcagcagg tgcggaagcg ccgcgcggac cgagtggatg gcgcccttca cgttgacttc
     8041 gaccatctcc tccaggtgct cctccgggag gtcgaggatc gggccatagg cgccgacgcc
     8101 ggcgttgacg accagcacgt cgattccgcc gaagcgctcg gcgaccgcgt tcgccgcgtc
     8161 gcgcatggac gcaccgtcgc ggacgtcgcc ccggatgccg atggcgtcgt ccagcccgag
     8221 gccgtcaccg ctgcgcgaga ccagcccgag gcgcatgccg gcgccgtgca gggaacgggc
     8281 ggtggccgcc ccgatgccgc ggctggcgcc ggtgatcatc gccactcgac cgtgcaggac
     8341 gtccattgcc tccgctcctg gcccgcgccg gccgagctcg gggacggcag cgccgggccg
     8401 ctcagggccg tcggtctatt gacacgcgcc gacggattgt acgactctac tccaaacgtt
     8461 tgagaaagag gaacacaaat ctttcgtgtc cgtgccgggt gacctcgtcg ggcagcgcag
     8521 cgagacggtc cggcgctcga acctgagcgc catcgtccgt gagctgcacg ttcgcggtcc
     8581 actctcgcgc tcggacctcg tgggcctgac cggcctcacg cgctccgcca tccgcggtct
     8641 gatcggcgag ctcgtagccg gcggcctggt gacggaagtg ccgaaccagc cggccggcac
     8701 cccgggccgt ccctcgccca tcgtccagcc aaacgcccgc ggcgccgtgg ccgtggccct
     8761 ggaggtgaac gtcgactcgc tcgccgccgc cctggtcggg ttcggcggca cgctgcacca
     8821 gctcgcgcgg gtcgaccggc cgcgcggcca cctctcggtc gacgcgatcg tcgacgacct
     8881 ggtcgagctc gtcggtccgc tgctgcgggc ggtcgacccg gatgcgctgg tggggatcgg
     8941 cgtggccgtc gccggcatcg tccggcgcag cgacggcatc gtgcgcatgg cgccgaacct
     9001 ggggtggcgc gacgttccgc tggcgcagcg gctgcgggag gcgctcccgg tcgtggcgcc
     9061 ggtgtgggtc gccaacgagg cggacctcgg aggcctggcg gagcaccgcc gcggcgcggc
     9121 catcggcgcc gacgaggtgc tctacgtgat gggagaggtc ggcgtcgggg gcaacatgat
     9181 cgtggacggc cggccgatca tcggcatcga cggctacgcc ggcgagatcg gccacatgtc
     9241 cgtcaacccc gacggcctgg cctgcgcctg cgggtcgcgc ggctgctggg agaccgagat
     9301 cggcgagcgg gcgctgctca ccagggcggg cttcgatccc gagggcgggc gcgaggcgat
     9361 cggcgaggtg atcgccgccg cgacggccgg ggagccgcgg gccgtgaccg ccgtcgagga
     9421 ggtcgggcgc tggctgggca tcggcatctc gtcgctggtg aacgtgctca acccgcagct
     9481 ggtcgtcgtc ggcggcgtgc tcgagcggct gtacccgctc atggcgcgca cgctcgagga
     9541 ggcggtggcc gtctacgcgc tgccgcccgg ccgtgagcac ctgcgcatcg tgccgggggc
     9601 cctgggggac gatgccccga ccctcggcgc cgccgagctc gccttcgagc cgctgctggc
     9661 ggacgcggct cttcagcttc gccttcggtc gcctgccgcg ctggtgagcg cgtgatgtgg
     9721 cgacatcgtc cgccgtccgg cgggtccacg atccgcgccg ctcgatgcgg ccctggagcg
     9781 cgacgaggag gggtttgaca ggagagtagc ctagtgctgg cacgccatcc ctcgggatgg
     9841 ccagagacct ggtacggagg aatctcaatg gtgatccgga ggcttgcggc cctcttggcc
     9901 gtgctggccg tcgtgcttgc ggcgtgtggt ggaaccggga ctgcctcggg ctccgcgtcg
     9961 gagccggcga gctcgggcgg tggcggcggg acgaccgact gcaccgtcgc cgtctcgtgg
    10021 aacaacttcc agcagccgcg ctgggcggcc gccgacgagc cgaacatcaa gcaggtcgtc
    10081 gaggacgcgg gcggcaccta catctcggct gatgccgacc tcagcagcga gcagcagctg
    10141 accgacgtca gcaacctcat cggtcagggc gccgatgtcc tgatcctcct tgcgcaggac
    10201 cagaacgccg tcctgccggc actccaggaa gccaaggacg ccggcatccc ggtgatcgcc
    10261 tatgaccggc tcatcgagga tcccgacatc ctgtacatca ccttcaacaa cgtcggcgtc
    10321 ggcgaggcgc aggcggccgc ggtcctcgag gccgtgcccg agggcaacta cgtcctcatc
    10381 aagggcgacc ccggcgaccc gaacgcctcg acgttcctgc cccagggctg ggacgacgct
    10441 ggcctgaagg agaaggtcga cgcgggtgac atcaccatcc tgaacgggcc ggaaggcggc
    10501 agcggcgagt ggccggccga ctacggcacg tacaccgatg cctgggacac ccagaccgcc
    10561 caggagaaca tggaagcgat catcgacgcc gccaacgctg acgacgtgac gatcgacgcc
    10621 atccttgccg agaacgacag cacggcgctc ggcgtcgtcg cggccctcga ggccaagtcg
    10681 tacggctacc cgcccctcag cgggcaggac ggcgatccgg ccaacctcaa caacgtcgcg
    10741 ctgggcaagc agtacgtcga cgtctggaag aacgccaacg agctcggcaa ggccgccggg
    10801 caggctgcca tccagatgtg ccagggcacc gcgatggccg atgtctcgat cgacatcgag
    10861 tcgtcggtgg ccccgaccga cggcaacagc ccgaccgact tcgagacccc gggtggcaac
    10921 accgtcaagt ccctgatcct gcagccgacg ccgctcacgg ccgaggatct ggacgtggtg
    10981 ctgagcgccg gctggtacgc gggcaaggat gagatctgcg ccggcgtcac cgagtccgac
    11041 ccgggctggg acgactgcca gtaagggtcg caggctccat gctgtgaccg aaccgtccgc
    11101 ctgcgccccc ggcccccggg ggcgcaggcc acatcgtccg gagtgaggag gagggtcctc
    11161 atggcgaacg tggccgacac cgcaccggtt cccggcgagt cccgcacggc gagcaccgcg
    11221 acgcgcatgc gggagatgct gtccgcgacc gaggtcgacg ttcgcctgtt cggcatgctg
    11281 gtggcgctcg ccgccatcct catcggcttc cagctgctcg acagcggctt cttcagcccc
    11341 gggaacatgg tcaccctggc ggtgcaggca attcccgtgg cgattctggc gaccggcatg
    11401 gtgctggtca tcgtgtcgcg caacatcgac ctgtcggtcg gctcgctgac cgggatcatc
    11461 gccatgacct acgcgatgct catgaccgat tggctgccga acatcctcgg cctgggcgtc
    11521 gacttcccgt tccgctggct catcgcgctc gtcatcggga tcgccgtcgg cgcgaccttc
    11581 ggcgcggtcc agggcttcat catcgcctac atcggcgtcc cgtccttcgt cgtcactctc
    11641 ggcggactgc tgtccatccg cggagcgatc tggtacttct ccaacggcgc cgccgtcacc
    11701 cggctcgatc ccaccttcca gctcatcggc ggcggcgcgc gcgggtccat cggcgagctg
    11761 ctgacctggg ggatcggtct gctcgggtgc gcgttcgtga tcttcgcgct gtggaacggg
    11821 cggcggcagc gacgtcgctt cgggttcccc ctgcgaccga tgtgggccga ggtcaccatc
    11881 ggcgtgctgg gctgtgcggc gatgctgggc ctggccgcgt tcgccaacgg caactacctg
    11941 ccggcgggcc tcgcaacgca gtacgcgcag gagcgtggca tcgtcgagcc acccggcggc
    12001 ctgaaggtgc cggccggctt cccgctgccc gtcatcctgc tgatcgcggt cacggtggtg
    12061 atgacctgga ttgcgacgcg ccgtcgcttc gggcgctacg tctacgccta cggcggcaac
    12121 cccgatgcgg ccgagctggc cggtatcaac acgcgccgca ccatcctgct gaccttcgtc
    12181 atcatgggca tcctggccgc gctctcggcc gcggtcgtcg cggcgcgcct gaacggggcc
    12241 acgctcgaca tcggcaacag ctacgagctg tatgtgatcg ccgcggccgt cgtcggcggc
    12301 acctcgttcg ccggcggcat cggcaccatc ccgggcgcgg tgctcggcgc gttcgtgatg
    12361 caggccctct cgtacggact gagcttcatc ggcgtgaact cgcccggcca gaacgtggtg
    12421 gccggcatcg tcctcatcct ggccgtggcg ttcgacacct ggaaccggcg tcgggccgcc
    12481 aggtagccac gcgagcatcg gagggagcca tcccgtgacc gcagccccgc agcccgccac
    12541 cacgccactc gtcgagatgc gcgacatccg ggtctcgttc ggcggcgtgc acgccgtggc
    12601 cggcgtcaac atcgacctcc acgaggggga gatcgtcggc ctggtcggcg gcaacggcgc
    12661 cgggaagtcg acgctgatga aggtgctgtc cggcgccctg gtcgccgact ccggcgaggt
    12721 gttcgtgtgc ggcgagcgcg cgcacctcgc aaacccgcgc gacgcgaagg cctacggcat
    12781 cgagaccatc taccagaccc tggcgcttgc cgacaacctg gacgctccgg ccaacgtgtt
    12841 cctgggccgc gagctgacca tgggcgtcgg ctcgctcgac gacagcgcca tggagaacgc
    12901 gacgcgcggc gtgatgcggc gtctcaaccc gaacttccag aacttcaaga ccccggtcgt
    12961 ctcgctctcg ggcgggcagc ggcagtcggt ggccatcgcg cgcgccgtcc agttcaacgc
    13021 caagatcctg atcatggacg agccgacggc cgccctcggg ccggccgaga ccacgcaggt
    13081 acgcaacctg atccggcagc tgaaggagga gggcatcggc atcttcctca tcagccacga
    13141 catccacgac gtctacgacc tgtcggatcg gatcagcgtc atgtaccacg gccgcatcgt
    13201 cgacaccgtg cgcaaggagg acgtcgacac cgatgcggtc ctcggcatga tcatcctggg
    13261 caagcgcccg ggcgatgtca cccaggagga gctcgcctcc ctgcgctgac gcggccggga
    13321 ccgatgcggg cgccgcgggc gcccg
//
LOCUS       DASTYP010000069         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_296327, whole genome shotgun sequence.
ACCESSION   DASTYP010000069 DASTYP010000000
VERSION     DASTYP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296327"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFV59_03530"
     CDS             <1..988
                     /locus_tag="VFV59_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006105100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU5324933.1"
                     /translation="EGTILIGTLGTLARPELAQKLAKRGVTAISMDAIPRITRAQSMD
                     TLSSQATVGGYKAVLIAAERLPKFFPLLTTAAGTIRPARGIVMGAGVAGLMAIGTARR
                     LGAIVEATDVRPVVKEQVESLGGTFIEVEMTEEEKAQAETSGGYAREMSDDYKRRQAE
                     LIAERVKEADFIITTALIPGRPAPKLVTEEMLRTMKPGSVIVDMAAEMGGNVEGTVPN
                     QEVVKHGVTLIGITNLPATMPGSATQMYAKNLQTLIKHLATDGRIELDFSDEITRGAT
                     ITHQGKVVNEATAKALGIEVSQPDAGGATAQQTDTPAAAPEAAPASVEKKDS"
     gene            994..1299
                     /locus_tag="VFV59_03535"
     CDS             994..1299
                     /locus_tag="VFV59_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU5324934.1"
                     /translation="MELTAFELLSALFILVLAAFVGYEVVSKVPTMLHTPLMSGANAV
                     HGVIVVGAMLVAATADGPIAIVLAFVAVVLGTINVVGGFTVTDRMLEMFRRRPEERG"
     gene            1300..2724
                     /locus_tag="VFV59_03540"
     CDS             1300..2724
                     /locus_tag="VFV59_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012306611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU5324935.1"
                     /translation="MSTLEILTFLVWLVASVTFIFALKFLASPKTARTGNLLGAAGMA
                     LIVLWTFFTHDATSPDAGPGMFGNWWIIAVGGAIGAVVGWLGAKRVAMTAMPQMVAIF
                     NGAGGGAAALVAVVEFLRDQELHPGELLPLAFIIATLLGAIIGSVSLTGSIVAWGKLQ
                     GVIASRPVRYPGSQWVTGLLFLVLIGLGVYVAVIEPSTPLFLLFVVLALILGVQLTMP
                     IGGADMPVVISLLNSYTGIAVAATGFVLSNYALLIAGTLVGASGAILTQLMCKAMNRS
                     LFNVLFAGFGTGGEAIAGAEGEETLKEISAEDAAVLMAYGKRVIIVPGYGMAVAQAQG
                     PVRELMDLLVSKGVDVQFAIHPVAGRMPGHMNVLLAEANVPYDRLQEMDEINDDFADT
                     DVALVIGANDVVNPVAREAGSPISGMPILNVDQAQHVIVLKRGRGAGFAGIPNPLFSL
                     DKTSMLFGNAKQTVEALVSGVKQA"
     gene            2852..3136
                     /locus_tag="VFV59_03545"
     CDS             2852..3136
                     /locus_tag="VFV59_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324936.1"
                     /translation="MQDEYVTFSAAWFTLALINAGIAQGKERSGFNWWLLSLFFGPLA
                     TLLLVLSGRGDPRRTESDSAGLTDRQARVLGVVLGVAVLALLGIFAFAVR"
     gene            3235..>3426
                     /locus_tag="VFV59_03550"
     CDS             3235..>3426
                     /locus_tag="VFV59_03550"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEU5324937.1"
                     /translation="MADILDSVTGLLDGQVGQVSQRIGADEAKTRTAIHAAVPALMAA
                     FAGEAQRGGGIKTAIRQDHD"
BASE COUNT          523 a         1189 c         1167 g          547 t
ORIGIN      
        1 tgaggggacg atcctgatcg gcaccctcgg caccctggcc cgaccggagc tggcccagaa
       61 gctggcgaag cgcggcgtca ccgccatcag catggacgcc atcccgcgca tcacgcgcgc
      121 gcagtcgatg gatacgctga gctcgcaggc aaccgtcggc ggctacaagg cggtcctcat
      181 cgccgccgag cggctgccca agttcttccc gctgctgacg accgccgccg gcaccatccg
      241 cccggcgcgg ggcatcgtca tgggcgccgg cgttgccggc ctcatggcca tcggcacggc
      301 gcgacggctc ggcgcgatcg tggaggccac ggacgtccgc ccggtggtga aggagcaggt
      361 cgagagcctt ggcggcacct tcatcgaggt cgagatgacc gaggaggaga aggcccaggc
      421 cgagacctcc ggtggctatg cccgcgagat gagcgacgac tacaagcgac gccaggcgga
      481 gctcatcgcg gagcgcgtca aggaagccga cttcatcatc accaccgcgc tcatccccgg
      541 acgaccggcg cccaagctcg tcaccgagga gatgctgcgg accatgaagc ccggcagcgt
      601 catcgtcgac atggcggccg agatgggcgg caacgtcgag ggcaccgtgc cgaaccagga
      661 ggtggtgaag cacggcgtca cgctcatcgg catcaccaac ctgccggcca cgatgccggg
      721 cagcgcgacg cagatgtacg ccaagaacct ccagacgctg atcaagcacc tggccaccga
      781 cggcaggatc gagctcgact tcagcgacga gatcacgcgc ggcgcgacga tcacccacca
      841 gggcaaggtg gtgaacgagg cgaccgcgaa ggcgctcggc atcgaggtgt cgcagcccga
      901 cgccggtggc gccactgccc agcagaccga tacgcccgct gcggcgcctg aggcggcgcc
      961 cgcatcggtc gagaagaagg actcgtaaga ccgatggagc tcaccgcatt cgagctgctg
     1021 tcggcgctct tcatcctcgt cctggccgcg ttcgtcggct acgaggtggt gtcgaaggtg
     1081 ccgaccatgc tccacacgcc gctgatgagc ggcgccaacg ccgtgcacgg cgtcatcgtc
     1141 gtcggcgcga tgctcgttgc cgcgaccgcg gacgggccga tcgcgatcgt cctggccttc
     1201 gtggccgtcg tcctcggcac catcaacgtc gtcggcggct tcaccgtcac cgaccggatg
     1261 ctcgagatgt tccggcgccg gccggaggag cgcggctaga tgagcacgct cgagatcctc
     1321 acgttcctcg tctggctcgt ggccagcgtc accttcatct tcgcgctgaa gttcctggcg
     1381 agcccgaaga ccgcccgcac cggcaacctg ctgggtgcgg ccggcatggc gctcatcgtc
     1441 ctgtggacgt tcttcacgca cgacgccacc agcccggatg ccggtcccgg catgttcggc
     1501 aactggtgga tcatcgccgt gggcggtgcc atcggcgccg tggtgggctg gctgggcgcc
     1561 aagcgcgtcg ccatgacggc gatgccgcag atggtcgcca tcttcaacgg cgctggtggt
     1621 ggcgccgccg cgctcgttgc cgtggtcgaa ttcctgcgcg atcaggagct gcaccccggc
     1681 gagctcctgc cgctggcctt catcatcgcc acgctcctgg gggcgatcat cggctccgtg
     1741 tccctgaccg gctcgatcgt ggcctggggc aagctgcagg gcgtcatcgc ctcccgcccg
     1801 gtccgctatc ccggctcgca gtgggtgacg ggcctgctct tcctggtgct catcggcctg
     1861 ggcgtgtacg tcgccgtcat cgagcccagc acgccgctct tcctgctgtt cgtggtcctg
     1921 gcgctgatcc tcggcgtcca gctcaccatg cccatcggcg gcgccgacat gcccgtcgtg
     1981 atcagcctgc tgaactcgta caccggcatc gcggttgccg cgaccggctt cgtgctcagc
     2041 aactacgcgc tcctgatcgc cggaacgctg gtcggtgcct ccggcgccat cctcacccag
     2101 ctcatgtgca aggcgatgaa ccgctcgctg ttcaacgtcc tgttcgcggg cttcggcacg
     2161 ggcggcgagg ccatcgccgg cgccgagggc gaggagacgc tgaaggagat cagcgcggaa
     2221 gacgccgccg tgctcatggc ctacggcaag cgcgtgatca tcgtgccggg ctacggcatg
     2281 gccgtcgcgc aggcccaggg tccggtgcgc gagctgatgg acctgctggt cagcaagggc
     2341 gtcgacgtgc agttcgccat ccaccccgtg gccggccgca tgccggggca catgaacgtg
     2401 ctcctggcgg aggcgaacgt gccgtacgac cggctgcagg agatggacga gatcaacgac
     2461 gacttcgccg atacggacgt cgccctcgtc atcggcgcca acgacgtggt caacccggtg
     2521 gcgcgcgagg ccggttcgcc gattagcggc atgcccatcc tcaacgtcga ccaggcgcag
     2581 cacgtgatcg tgctgaagcg cggacgcggc gccggcttcg ccggcatccc gaacccgctg
     2641 ttcagcctgg acaagacgtc catgctgttc ggcaacgcca agcagaccgt cgaggcgctg
     2701 gtctcgggag tgaagcaggc gtagcgcacc gttcgcttct gaccgtcgcg ctgggcgggt
     2761 ggtccttcgg ggccgcccgc ccttgtcgcg tgctggcttc gacgctgtcc gtccgccgct
     2821 cggcgtgcca ctccagcggt atggtgacga catgcaggat gagtacgtca cgttctcggc
     2881 cgcctggttc acgctcgcac tcatcaacgc cgggatcgcc cagggcaagg agcgttcggg
     2941 tttcaactgg tggctgctgt cgctattctt cggcccgctc gcgaccctgc tgctcgtgct
     3001 gtcgggccgc ggtgatccac gccggacgga gtccgactcg gcgggattaa cggaccggca
     3061 ggcgagggtt ctcggcgtcg tcctgggcgt cgcggtgctg gcactcctgg gcatcttcgc
     3121 gtttgccgtg cgctgaggtt tgctggctgc gccccggcgc caatcgtgct agagctatcg
     3181 gtgtgctcgc gcgccgcgtg agtctcgaca ggaccacgaa gggagccact caacatggca
     3241 gacatcctcg attcggtcac cggcctgctg gatgggcagg tcgggcaggt cagccagcgc
     3301 atcggtgccg atgaggccaa gacgcggacc gcgatccacg ccgccgtccc ggcgctgatg
     3361 gccgcgttcg cgggcgaggc ccagcgaggc ggcggcatca agacggccat ccgccaggac
     3421 cacgat
//
LOCUS       DASTYP010000070         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1425673, whole genome shotgun sequence.
ACCESSION   DASTYP010000070 DASTYP010000000
VERSION     DASTYP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425673"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFV59_03555"
     CDS             complement(<1..504)
                     /locus_tag="VFV59_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5324938.1"
                     /translation="MSDPVGLVVLGAGDRGRGTYGRWCLEHPDRARVVAVADPDDERR
                     AALAAEHGIPASATAVDWRDLLARPRLADAVVVATPDREHAAATIAALGLGYDVLLEK
                     PIAPTLDEVRAVAEAAARSAGTVTVAHVLRYTPFFSAVKQAIEAGWIGELVTMAHTEH
                     IGYWHFAH"
     gene            complement(501..782)
                     /locus_tag="VFV59_03560"
     CDS             complement(501..782)
                     /locus_tag="VFV59_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324939.1"
                     /translation="MKGDRVEIVVDTGQGGTQTFEIEATRAGRRIEVTTARGVVEVSE
                     VTRGGTPVRTGRFMTSRVVALVEHPAREGAELPVEVSTRRRMSAGSDAG"
     gene            complement(782..2995)
                     /locus_tag="VFV59_03565"
     CDS             complement(782..2995)
                     /locus_tag="VFV59_03565"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5324940.1"
                     /translation="MAQLARFSPAAVVQRITRGGEVAGASEPTIEGIIAEAAKHRPNL
                     DAQLIRRAYETAVAAHESQQRSSGEAYVSHPVAVTYYLATLQMDAETLAAALLHDVPE
                     DTDYTLAEIEKRFGRTVARLVDGVTKLSKFGSARTMEEQQAENIRKMFMAMAEDVRVV
                     IIKLADRLHNMRTLQYLPEEKQARIARQTMEIYAPLAHRLGMWQIKWELEDLAFKYLE
                     PEKYRQLVEMLADRRRSRESFVNKSIGILRKELEKVSIKAEISGRPKHLYSIAKKMER
                     KGAEFTEIYDLNAIRVLVEDVKDCYAALGVVHSLWRPVPGQFDDYIAMPKANMYQSLH
                     TAVIGPEAKPLEVQIRTYRMHEVAEAGIAAHWRYKEGSRGDRRYDEKLAWVRQLMDWQ
                     REVSDPTEFVEGLKLDLFQDQVFVFTPKGDVKDLPRDATPLDFAYRIHTDVGHRTIGA
                     KVNNRLVPLDYRLRNGDIVEIVTTKAAHGPSRDWLNIVRTSHAREKIRAWFKRQQRDE
                     NITQGKDLLDRELRRLAHETLASVDAAKIAEIASQYRYREVDDFYAAIGYGAITPSSV
                     VSKLEIHDDAAITLPEEAPPAAPSTTGVRVKGVGDLLVRFAKCCSPIPGDQITGYVTR
                     GKGVTVHRSSCPSVLSERDIERLIDVEWELAGQQTYPITVRISALDRPGLLHEVTNVV
                     AEYKVNIVAASVATHADGTATITATFKVTSLQQLAKVLTKIERLRDVTSVTREAR"
     gene            complement(3051..3548)
                     /locus_tag="VFV59_03570"
     CDS             complement(3051..3548)
                     /locus_tag="VFV59_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU5324941.1"
                     /translation="MADDELLHPTVRDALHRLRVEHEVMPCDPALADTAAFVDAYGVP
                     ADRSANTIVVASKGAEPRHVACVLLATTSLDVNNVVRREMGVRKASFAGADPVRELTG
                     MEIGGVTPFGLPPEVPILVDARVMAPDWIILGGGNRSSKLRLAPDALRAVPSLRVVEG
                     LAIQR"
     gene            complement(3552..>4232)
                     /locus_tag="VFV59_03575"
     CDS             complement(3552..>4232)
                     /locus_tag="VFV59_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEU5324942.1"
                     /translation="PKVQPPTTRVVDLTVGEEELRSNLKRKHRQYVNKAERAGVTVER
                     FDGSTPPDLIGPALADFNRIYQLTADRAGFVARQPFYYERVWSIFAPTGRVRLSFAML
                     DGHRVATLFHFTCGERVVESYGGMTDAGADARANYLLKWTAMADFAREGFAVYDMWGL
                     ATGGIRQFKEGFGGHEIEYVGARDLGLRAPVDAALRIAIPAYGLAQRARLKLLGRDRG
                     APEASEVT"
BASE COUNT          575 a         1486 c         1486 g          685 t
ORIGIN      
        1 gtgggcgaag tgccagtagc cgatgtgctc ggtgtgcgcc atcgtcacca gctcgccgat
       61 ccaccccgcc tcgatcgcct gcttcacggc cgagaagaac ggggtgtagc gcaggacgtg
      121 cgcgacggtc accgtgccgg cggagcgcgc cgcggcctcc gcgacggccc gcacctcgtc
      181 cagggtcggc gcgatgggct tttccaggag cacgtcgtag cccaggccga gcgccgcgat
      241 ggtcgcggcc gcatgctccc ggtcgggcgt ggccacgacc accgcatccg ccaggcgggg
      301 ccgcgccagg aggtcgcgcc agtcgaccgc cgtcgcgctg gccgggatgc cgtgctccgc
      361 ggccaatgcc gcgcgccgct cgtcatccgg atctgccacg gcgacgaccc gggcgcgatc
      421 cgggtgctcc aggcaccagc gaccgtacgt gccgcggccg cggtcgccgg cgccgaggac
      481 gaccaggccg acggggtcgc tcaaccggcg tcggagccgg cgctcatgcg acggcgcgtc
      541 gagacctcga ccggcagctc cgcgccctcg cgggccgggt gctcgaccag cgccaccacc
      601 cggctggtca tgaagcggcc ggtgcgcacc ggcgttccgc ctcgtgtcac ctcgctgact
      661 tccacgacgc cgcgagcggt ggtcacctcg atccggcggc cggctcgcgt ggcctcgatc
      721 tcgaaggtct gggtgccgcc ctggccggtg tcgaccacga tctcgacgcg atcgcccttc
      781 atcagcgtgc ctcccgcgtc acgctggtga cgtcccggag gcgctcgatc ttggtcagca
      841 ccttggccag ctgctggagc gacgtcacct tgaaggtcgc ggtgatggtc gcggtcccgt
      901 cggcgtgcgt cgcgaccgag gccgccacga tgttgacctt gtactcggcc accacgttcg
      961 tgacctcgtg cagcagcccc ggccggtcca gcgcactgat ccgcaccgtg atcgggtacg
     1021 tctgctgccc ggccagctcc cactccacgt cgatgagccg ctcgatgtcc cgctccgaca
     1081 gcaccgaggg acacgacgag cgatgcacgg tcacgccctt cccgcgcgtc acgtacccgg
     1141 tgatctggtc tcccgggatc ggcgagcagc acttggcgaa ccggaccaac aggtccccga
     1201 ctcccttgac ccgcacgccg gtggtggagg gggcggccgg cggcgcctcc tccgggaggg
     1261 tgatggccgc gtcgtcatgg atctcgagct tgctgacgac gctggacggc gtgatggcgc
     1321 cgtagccgat ggcggcgtag aagtcgtcaa cctcgcggta gcggtactgg gacgcgatct
     1381 cggcgatctt cgcggcatcg accgaggcca gcgtctcgtg cgccaggcgg cgcagctccc
     1441 ggtcgagcag gtccttgccc tgggtgatgt tctcgtcgcg ctgctggcgc ttgaaccagg
     1501 cgcggatctt ctcgcgcgcg tgcgacgtgc gcacgatgtt cagccagtcc cggctcggtc
     1561 cgtgcgccgc cttcgtcgtc acgatctcga cgatgtcgcc gttgcggagc cggtagtcga
     1621 gcggcaccag gcggttgttg accttggcgc cgatggtgcg gtggccgaca tcggtgtgga
     1681 tgcggtaggc gaagtcgagc ggcgtcgcgt cgcgtggcag gtccttgaca tcgcccttcg
     1741 gcgtgaacac gaagacctgg tcctggaaca ggtcgagctt cagcccctcg acgaactcgg
     1801 tggggtcgct gacctcgcgc tgccagtcca tgagctgccg gacccaggcc agcttctcgt
     1861 cgtagcggcg gtcgccgcgg ctgccctcct tgtaccgcca gtgcgcggcg atgcccgcct
     1921 cggccacctc gtgcatgcgg taggttcgga tctgcacctc gagtggcttc gcctccggac
     1981 cgatgacggc ggtgtgcagc gactggtaca tgttcgcctt gggcatggcg atgtagtcgt
     2041 cgaactgccc cgggaccggg cgccacagcg agtgcaccac gcccagggcc gcgtagcagt
     2101 ccttgacgtc ctcgaccagc acccggatgg cgttcaggtc gtagatctcg gtgaactcgg
     2161 cgcccttgcg ctccatcttc ttggcgatcg agtacaggtg cttggggcga ccggagatct
     2221 cggccttgat gctgaccttc tccagctcct tgcgcaggat gccgatgctc ttgttgacga
     2281 agctctcgcg ggaccgacgc cgatccgcga gcatctcgac cagctggcgg tacttctccg
     2341 gctcgaggta cttgaaggcg aggtcctcga gctcccactt gatctgccac atgcccagcc
     2401 gatgcgccag cggcgcgtag atctccatgg tctggcgcgc gatgcgggcc tgcttctcct
     2461 ccggcaagta ctgcagggtg cgcatgttgt ggagccgatc ggccagcttg atgatgacga
     2521 cccggacgtc ctcggccatg gccatgaaca tcttccggat gttctcggcc tgctgctcct
     2581 ccatggtccg ggcgctgccg aacttgctga gcttggtgac cccgtccacc aggcgggcga
     2641 ccgtgcgccc gaagcgcttc tcgatctcgg ccagcgtgta gtcggtgtcc tccgggacat
     2701 cgtgcagcag cgccgcggcc agcgtctcgg catccatctg cagcgtcgcc aggtagtacg
     2761 tcaccgcgac cgggtggctg acgtacgcct cgcccgagct gcgctgctgg ctctcgtggg
     2821 cggcgaccgc cgtctcgtag gcgcgccgga tgagctgcgc gtcgaggttc gggcggtgct
     2881 tggcggcctc ggcgatgatg ccttcgatcg tcggctcgga ggcgcccgcc acctcgccgc
     2941 cgcgcgtgat gcgttgcacg acggccgccg gggagaagcg cgccagctga gccatgggat
     3001 cgagatgcta gcacgcggat cggcccaccc cgcgcagccg atggggtcgc tcagcgctgg
     3061 atcgcgagcc cctccaccac gcgcagcgac ggcacggcgc gcagcgcatc gggcgccagg
     3121 cgcagcttcg acgagcggtt cccaccgccc aggatgatcc agtcgggggc catgacccgc
     3181 gcgtcgacca ggatcggcac ctcgggcggc agcccgaagg gcgtcacgcc accgatctcc
     3241 atgccggtca actcgcgcac cggatcggcg ccggcgaagg acgccttgcg gacgcccatc
     3301 tcgcgacgca ccacgttgtt gacgtcgagc gacgtcgttg cgaggaggac gcacgcgacg
     3361 tggcgcggct cggcgccctt cgaggcgacc acgatcgtgt tggcgctgcg gtccgccggg
     3421 acgccgtagg cgtcgacgaa ggctgccgtg tcggccaggg cggggtcaca gggcatgacc
     3481 tcgtgctcga cccttagccg gtgcagcgcg tcgcggaccg tggggtgcag cagctcgtcg
     3541 tccgccatcg gtcaggtcac ctccgacgcc tctggcgcac cccgatcgcg accgaggagc
     3601 ttgaggcgcg cgcgctgcgc gaggccgtag gccgggatgg cgatccgcag ggcggcgtcg
     3661 accggggccc gcagcccgag gtcgcgggca ccgacgtact cgatctcgtg gccgccgaag
     3721 ccctccttga actgccggat gccgccggtg gccaggcccc acatgtcgta caccgcgaag
     3781 ccctcgcgcg cgaagtcggc catggcggtc cacttcagga ggtagttggc gcgggcgtca
     3841 gcgcccgcat cggtcatgcc tccgtaggac tcgaccacgc gttcaccgca ggtgaagtgg
     3901 aagagggtgg cgacccggtg gccgtcgagc atcgcgaacg agaggcggac gcggccggtc
     3961 ggggcgaaga tcgaccacac ccgctcgtag tagaagggct gccgggcgac gaagccggcc
     4021 cggtcggccg tcagctggta gatccggttg aagtcggcga gcgccggacc gatgaggtcg
     4081 ggtggcgtgg agccatcgaa ccgctcgacg gtgacgccgg cccgctctgc cttgttgacg
     4141 tactggcggt gcttgcgctt caggttcgac cgcagctcct cctcccccac cgtcagatcg
     4201 acgacgcggg tggtcggcgg ctgcaccttg gg
//
LOCUS       DASTYP010000071         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_368923, whole genome shotgun sequence.
ACCESSION   DASTYP010000071 DASTYP010000000
VERSION     DASTYP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368923"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VFV59_03580"
     CDS             complement(<1..1022)
                     /locus_tag="VFV59_03580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU5324943.1"
                     /translation="MTLRITVLGTGYLGATHAACMAELGFEVLGMDVDQAKVDSLMDG
                     RMPFYEPDLEPLLRKHVESGRLRFTTSYEEVAAFGDVHFLCVGTPQRKGEYAADMRFV
                     DGVVDGLAPHLDRPTLVVGKSTVPVGTAARLAPRIADLAPAGEQAELAWNPEFLREGY
                     AVEDTLRPDRLVVGTTSERAEATLREVYASAIDAGTPFLVTDFATAELVKVSANAFLA
                     TKISFINAMAELCEATGADVTVLADAIGHDERIGRKFLNAGLGFGGGCLPKDIRAFMA
                     RAGELGVDQALTFLKEVDAINMRRRGHVVDRARAMCGGSFLGTRVAVLGAAFKPNSDD
                     VRDSPAL"
     gene            1090..2241
                     /locus_tag="VFV59_03585"
     CDS             1090..2241
                     /locus_tag="VFV59_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5324944.1"
                     /translation="MSDFDLFRLSDEHQMLREAVRAVCDDKIAPLAAEVDETGEFPQK
                     SYDALRKADFHAVHIPQEYGGAGADALATCIVIEEVARVCASTSLIPAVNKLGTTPLL
                     LSASQDLLKRYLPAVASGEAMFSYALSEPEAGSDAVAMRTTAARDGDSYVLNGAKRWI
                     TNAGVSDYYTVMAVTDTDAGANGVSAFVVEKADEGFTFGAPEKKLGIKGSPTRELYFD
                     DCRIPADRLVGAEGSGFKTALRTLDHTRVTIAAQAVGIATGALEYATRYVKERKQFGK
                     AIAEFQGIQFMLADMAMKLEVARQMTYVAAAKSERGDSDLTFFGAAAKCFASDTAMEV
                     TTDAVQLLGGYGYVKDYPVERMMRDAKITQIYEGTNQIQRLVMARKLLK"
BASE COUNT          400 a          860 c          924 g          359 t
ORIGIN      
        1 agggcggggg agtcgcggac gtcgtcggag ttcggcttga acgccgcgcc gagcaccgcg
       61 acgcgggtgc cgaggaagga cccgccgcac attgcccgcg cccggtccac gacgtgaccg
      121 cggcggcgca tgttgatcgc gtcgacctcc ttgaggaagg tcagcgcctg gtcgaccccg
      181 agctcgccgg cgcgggccat gaaggcccgg atgtccttcg gcaggcatcc gccgccgaag
      241 ccgaggccgg cgttgaggaa cttgcggccg atccgctcgt cgtgaccgat cgcgtccgcg
      301 aggaccgtga cgtcggcgcc ggtcgcctcg cacagctcgg ccatcgcgtt gatgaacgag
      361 atcttggtgg cgaggaaggc gttggccgac accttgacca gctcggcagt cgcgaagtcg
      421 gtgaccagga agggcgtgcc ggcgtcgatg gcggaggcgt agacctcccg cagggtggcc
      481 tcggcgcgct cggaggtggt cccgacgacg agtcggtcgg ggcgcagggt gtcctccacc
      541 gcgtagccct cgcggaggaa ctccgggttc caggccagct cggcctgctc gccggccggc
      601 gcgaggtcgg cgatgcgcgg ggccagccgg gccgcggtgc cgacgggcac ggtcgacttc
      661 ccgacgacca gcgtcgggcg gtccaggtgc ggtgccaggc cgtcgaccac gccgtccacg
      721 aagcgcatgt cggcggcgta ctcacccttg cgctggggcg tcccgacaca gaggaagtgg
      781 acgtctccga aggcggccac ctcctcgtac gacgtggtga agcgcagccg gccgctctcc
      841 acgtgcttgc gcagcagcgg ctccaggtcg ggctcgtaga acggcatccg gccgtccatc
      901 agcgagtcga ccttcgcctg gtccacgtcc atgccgagca cctcgaagcc cagctcagcc
      961 atgcaggccg cgtgggtggc gccgaggtaa ccggtgccga ggacggtgat gcgcaacgtc
     1021 atgacaggag aggctagcga gcctcacccg ggcgtcgtct cctggacgcg cgtgcgtaac
     1081 ctctgcagcg tgagtgactt cgaccttttc cgcctctccg acgagcacca gatgttgcgt
     1141 gaggcggtgc gcgcggtctg cgacgacaag atcgcgccgc tggctgccga ggtggacgag
     1201 accggggagt ttccgcagaa gtcctacgac gcgctgcgca aggcggactt ccacgccgtg
     1261 cacatcccgc aggagtacgg cggcgccggc gccgacgcgc tcgcgacctg catcgtgatc
     1321 gaggaggtcg cccgggtctg cgcctcgacg tcgctcatcc cggccgtgaa caagctgggc
     1381 accacgccgc tgctgctctc ggcgtcgcag gacctgctga agcggtacct gccggcggtc
     1441 gccagcggcg aggcgatgtt ctcctacgcg ctgtccgagc cggaagccgg cagcgacgcc
     1501 gtggcgatgc gcaccacggc cgcccgggac ggcgactcgt acgtcctcaa cggggcgaag
     1561 cgctggatca ccaacgccgg cgtctccgac tactacaccg tgatggcggt caccgacacc
     1621 gacgccgggg cgaacggcgt ctccgccttc gtggtcgaga aggcggacga gggcttcacc
     1681 ttcggtgcgc cggagaagaa gctcggcatc aaggggtcgc cgacgcgcga gctgtacttc
     1741 gacgattgcc ggatcccggc cgaccggctc gtgggcgcgg agggcagcgg cttcaagacc
     1801 gcgctgcgca cgctggacca cacccgcgtc accatcgcgg cgcaggctgt ggggatcgcg
     1861 accggggcgc tcgagtacgc gacgcgctac gtcaaggagc gcaagcagtt cggcaaggcc
     1921 atcgcggagt tccagggcat ccagttcatg ctcgccgaca tggcgatgaa gctcgaggtg
     1981 gcccggcaga tgacctacgt cgcggcggcg aagtcggagc gcggcgacag cgatctgacg
     2041 ttcttcgggg cggcagcgaa gtgcttcgcg tccgacactg cgatggaggt cacgaccgac
     2101 gcggtgcagc tgctgggcgg ctacggctac gtgaaggact acccggtcga gcggatgatg
     2161 cgcgacgcca agatcaccca gatctacgag ggcaccaacc agatccagcg tctggtgatg
     2221 gcccgtaagc tcctgaagta gcagcgccgg gggcatcggc accccttgca tgacacaggt
     2281 gtgacggcgg cggacgtctc gcagggcccc gccatgggaa cggactgttg cccccacagc
     2341 ggacgtaggc ccggtacgca cgacgcatgg cgcgcggctg atcacgtccc tctacggttc
     2401 gcccaggact caacgggagg ggcagcatgc tgctgttcgg aggcaggcga ggggcgaggt
     2461 cggcactgtt gctcaccgtg tcggtgctgg cgaccgccgg tgccctgctg acggcaccgg
     2521 ccgccccggc ggccacccgg acc
//
LOCUS       DASTYP010000072         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1790036, whole genome shotgun sequence.
ACCESSION   DASTYP010000072 DASTYP010000000
VERSION     DASTYP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790036"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..580
                     /locus_tag="VFV59_03590"
     CDS             50..580
                     /locus_tag="VFV59_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU5324945.1"
                     /translation="MHQPLLPYTPERDVYRLLGVPSSASSDEIQAACRRLARTFHPDR
                     NVSDRANQEMQVVNAVRRVMTDPGSRAAYDRERARFHATRARFVTAVATAQPGPVPRP
                     TEQPAPRETERQTAPILPPAVNRYLRAAGIGVRALLAALSPARCRTCRIVIDERDTFC
                     AACGTKLLTTGAPQAR"
     gene            complement(797..3061)
                     /gene="aceE"
                     /locus_tag="VFV59_03595"
     CDS             complement(797..3061)
                     /gene="aceE"
                     /locus_tag="VFV59_03595"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU5324946.1"
                     /translation="MSERSRCSTPWSPGIYARAFLEGRLTEEQMKRFRTEASGPGLSS
                     YPHPWLMPGFWQFPTVSMGLGPLMAIYQARFMKYLTGREISDTSTRKVWAFMGDGEMD
                     EPESMGSISLAGRERLDNLVFVVNCNLQRLDGPVRGNGKIIQELEALFRGAGWHVIKV
                     IWSRDWDPLLARDVDGVLVNKMNETLDGDYQRMAVSDGATIREQFFGPDPRLRKLVED
                     IPDDDLAKLRRGGHDYRKVYAAYAAAVAHKGAPTVILAKTVKGWTLGPGIEGRNITHQ
                     AKKLTADELKVFRDRLELPIPDDRLKEAPYYHPGPEAPEIQYMLERRRQLGGSVPKRT
                     VVPKALPSPKDAAFSEFFAGSDRPASTTMAFSRLIRNLVRDEQMGRRVVPIIPDEART
                     FGMDPLFKEVGIYNPLGQRYTPVDKELLLSYVESDTGQLLEEGITEAGAMSSLQAAAT
                     SYATWREPMVPFYTFYSMFGMQRTGDQVWALGDARGRGFMMGATAGRTTLTGEGLQHD
                     DGQSHVLASAVPNIRAYDPAFAYELAIIVRDGIQRMYGEAPEDVFYYITLYNENWAQP
                     AKADGVEDGVIRGLYRFREASGGTRKAQILASGPIMQQALRAQQVLAEKFDVAADVWS
                     ATSFQLLRNEALDVERWNRLHPDAEPRVPYVRQCLAPTEGPVIAATDYMKALPDMVAP
                     WIDRAYNVLGTDGFGRSDTRDALREFFEVNAEHIVYATLHGLCREGRATPDELRRAID
                     ELGIDADRANPLYS"
BASE COUNT          457 a         1124 c         1087 g          489 t
ORIGIN      
        1 gtgccgcgct gcgagagctc ggggaacgcg gtaggatgcc cgcggaccga tgcaccagcc
       61 cctgctgccg tacacccccg agcgcgacgt ctaccgcctg ctgggcgtcc cctccagcgc
      121 ctcctccgac gagatccagg ccgcctgtcg ccgcctggcg cgcaccttcc atccggatcg
      181 gaacgtgtcg gaccgggcca accaggagat gcaggtcgtc aacgcggtgc gtcgcgtcat
      241 gaccgatcct ggcagccggg ctgcgtacga ccgcgagcgc gcccgcttcc acgccacgcg
      301 ggcgcgcttc gtaacggccg tcgcgaccgc tcagcccggg ccggtaccac ggccgaccga
      361 gcagccggcc cctcgcgaaa cggagcgtca gacggcgccg atcctgccgc cggcggtcaa
      421 ccgctacctt cgagcggccg gcatcggcgt ccgcgcgctg ctcgcggcgc tctcaccggc
      481 gcgttgccgc acctgccgca tcgtgatcga cgagcgcgac accttctgcg ccgcctgcgg
      541 cacgaagctc ctcaccaccg gagcccccca ggctcgctag ctcgcgcgcc ctcgatctat
      601 cgagccagac tcacgcgagg cggccgccgc gtgccgttct cgtgatgaat cgagcgctga
      661 ccagcgcgaa ggcggcggcg ccgcccagct cgcgcgacac gagtcccggt ctatcgcgcg
      721 agcgtcacac aaggcacgac cgcggcgaag atgcctcgac gcgtcgacac gcccagccgc
      781 agagaggagg aggcggtcag gagtacagcg ggttggcgcg gtcggcgtcg atccccagct
      841 cgtcgatcgc gcgacgcagc tcgtccggcg tcgcgcgtcc ctcgcggcag aggccgtgca
      901 gcgtggcgta cacgatgtgc tcggcgttga cctcgaagaa ctcgcgcagc gcgtcgcgcg
      961 tatcggaccg gccgaagcca tccgtgccga ggacgttgta ggcgcggtcg atccacggcg
     1021 cgaccatgtc gggcagcgcc ttcatgtagt cggtcgcggc gatcaccggg ccctcggtcg
     1081 gcgccaggca ctgccggacg tacggcacgc gtggctccgc atcggggtgg aggcggttcc
     1141 agcgctcgac gtccagtgcc tcgttgcgca gcagctggaa gctcgtcgcg ctccagacat
     1201 cggcggcaac gtcgaacttc tcggccagca cctgctgcgc gcgcagcgcc tgctgcatga
     1261 tcggtccgga ggcgaggatc tgggcctttc gcgtcccacc cgacgcttcc cggaaccgat
     1321 acaggccgcg gatgacgccg tcctcgacgc cgtcggcctt ggccggctgc gcccagttct
     1381 cgttgtagag ggtgatgtag tagaagacgt cctcgggcgc ctcgccgtac atgcgctgga
     1441 tgccgtcgcg gacgatgatc gccagctcat aggcgaaggc ggggtcgtac gcgcggatgt
     1501 tcgggacggc gctggccagg acgtgcgact gcccgtcgtc gtgctggagg ccctcgccgg
     1561 tcagcgttgt gcggcccgcc gtcgcgccca tcatgaagcc acgaccgcgc gcgtcgccca
     1621 gcgcccagac ctggtcgccg gtccgctgca tcccgaacat cgagtagaag gtgtagaacg
     1681 ggaccatcgg ctcgcgccac gtcgcgtacg aggtggcggc cgcctgcagg ctgctcatgg
     1741 cgccggcctc ggtgatgccc tcctccagca gctgacccgt atcggactcg acgtagctga
     1801 ggagcagctc cttgtcgacc ggcgtgtagc gctgcccgag cgggttgtag atgcccacct
     1861 ccttgaacag cgggtccatg ccgaaggtgc gagcctcgtc ggggatgatc gggaccaccc
     1921 gacggcccat ctgttcgtcg cgcaccaggt tccggatgag ccggctgaac gccatggtgg
     1981 tgctggccgg ccggtccgag ccggcgaaga actcgctgaa ggcggcgtcc ttcggggagg
     2041 gcagcgcctt cggcaccacg gtccgcttgg ggaccgatcc gccgagctgc cgccgtcgct
     2101 ccagcatgta ttggatctcc ggggcttcgg ggccggggtg gtagtaggga gcctccttga
     2161 gccgatcatc cgggatcggc agctccagcc gatcacggaa gaccttcagc tcatcggccg
     2221 tgagcttctt ggcctggtgg gtgatgttgc gtccctcgat gcccgggccg agcgtccagc
     2281 ccttgaccgt cttggccagg atcaccgtcg gggcaccctt gtgcgctacc gcggccgcgt
     2341 aggccgcgta gaccttgcgg tagtcgtggc cgccgcggcg gagcttggcc aggtcgtcgt
     2401 ccgggatgtc ctcgaccagc ttgcgcaggc gcggatccgg gccgaagaac tgctcgcgga
     2461 tggtcgcgcc atcgctgacg gccatgcgct ggtagtcgcc gtcgagcgtc tcgttcatct
     2521 tgttgaccag cacgccgtcg acgtcgcgcg ccagcagcgg atcccagtcg cgcgaccaga
     2581 tgaccttgat gacgtgccag ccagccccgc ggaacagcgc ctccagctcc tggatgatct
     2641 tgccgttgcc gcgcaccggc ccgtcgaggc gctggaggtt gcagttgacg acgaagacga
     2701 ggttgtcgag gcgctcgcgg ccggcgagcg agatcgagcc catcgactcg ggctcgtcca
     2761 tctcgccgtc gcccatgaac gcccagacct tccgcgtgct cgtgtcgctg atctcgcggc
     2821 cggtcaggta cttcatgaac cgcgcctggt agatcgccat gagcgggccg aggcccatcg
     2881 acaccgtcgg gaactgccag aagcccggca tgagccacgg gtgggggtac gacgacagcc
     2941 cggggccgga cgcctcggtc cggaagcgct tcatctgctc ctcggtgagc cggccctcga
     3001 ggaatgcgcg ggcgtagatc cccggcgacc agggcgtcga gcaccggctg cgctcgctca
     3061 tgcggtggaa cgcgatcgcc accgtgttgc aggccaacgc cgagtcgtcc gagctcggcg
     3121 gccacatcgc cagctaccag tccgcggcga cgctcta
//
LOCUS       DASTYP010000073        16495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_78184, whole genome shotgun sequence.
ACCESSION   DASTYP010000073 DASTYP010000000
VERSION     DASTYP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16495
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78184"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..686)
                     /locus_tag="VFV59_03600"
     CDS             complement(36..686)
                     /locus_tag="VFV59_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5324947.1"
                     /translation="MPSRERIRAIGIQQASYLRSQLGREARTLRMRAGISQAELARTI
                     GVSRGWVARLELGRLGSVDLARSARLFACLGHKLSVKMYPVGEPLRDAGQARLLARFN
                     ARVSPAWHRTFEVVMPIRGDLRAWDEVLSGLVRIGVEAETRPANLEATQRSISAKQRD
                     SGVERTILLLAATHANRTLVRNHIGALRQSFPLDTRTTLAALSAGLDPGANGLVLL"
     gene            972..2135
                     /locus_tag="VFV59_03605"
     CDS             972..2135
                     /locus_tag="VFV59_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5324948.1"
                     /translation="MNEAISNIWRRPLRTALTILGIVIGVFALTVMGALAEKLNVLVD
                     GGDEYFRTRLFVFDEGAASGFQASSRPITRELVDQIEQIDGVERATPVLQTLLDPDEV
                     GGFDIPELLTGIEPETWYDDSVVLGVDAGRLLEAGDEGVTTIGADLAERYDLRVGDTF
                     TIRERDFEVIGVLERTLTFPDKIAYVPLADAQQIYVQDVPEGFEERTGDLATQIEVFP
                     NDLSEADAIAEAIDEQVDGVRAITPQQVADQIGQASIVFNLIIVGASVIAVIVGGLSV
                     INTMVMTVSERVREIGIKKAVGAPTSAILREFVVEATLLGAIGGLIGLGAGALLVVIL
                     NAQTAGSGTTVFLLTPMLLLRSFLFATILGALAGIYPALRAARLDPVRALRST"
     gene            2146..2946
                     /locus_tag="VFV59_03610"
     CDS             2146..2946
                     /locus_tag="VFV59_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018797570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324949.1"
                     /translation="MPEPILSARQLTKVYRRGASEVRAVDGVDLDVAPGEFMAIMGRS
                     GSGKTTLLDLLGCLLRPTSGELTIDGRSVIGASDGELAALRRQRIGFVFQEFNLIPTL
                     TAVENVLLPLRYGPRRPDGRRRASELLDLVGLAERATHRPTELSGGEQQRVAIARALV
                     NEPAVILADEPTGELDSATSERLMATLRELNRERGVTTIIVTHDAGVAGATDRVVRLQ
                     DGHVLADARRNGGNGRAFDAALTGQPHTDGGGRVWARLGDLLDRARGR"
     gene            2943..3917
                     /locus_tag="VFV59_03615"
     CDS             2943..3917
                     /locus_tag="VFV59_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5324950.1"
                     /translation="MIEAIGLAKQFRTLTAVHDLSFAVGDGEIFGLLGPNGAGKTTSI
                     RMLAGLIAPSAGTARVNGLELGPQSQRIRALTGVLTESPGLHDRLTARQNLGYYGRLY
                     GLRGGELRRAVDRYLGIVGLTPAADRRVGGFSKGMRQKVAIARALLHEPQVLYLDEPT
                     SALDPSAAKGVRDFVATLRDAGRSIVVCTHNLDEAERLCDRIGIMRGTLLTVDTPARL
                     RRRNGTATVRVELVGARRPTSFLDLLGRLAFVEGARAVDGALLVEVREPRGDNPELVR
                     ELVEAGARIVHVSEEAATLEQVYLDLVGEAGARDAGAGIGLDSDGEAG"
     gene            3917..4708
                     /locus_tag="VFV59_03620"
     CDS             3917..4708
                     /locus_tag="VFV59_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5324951.1"
                     /translation="MRPGYVWAVIGKEARDLLSNRLLLGAVVFPALVFAAIPTGIVAF
                     IEFNELDPDQLGQIEQYISQFPDLPAKLAAQGFIVMNFMAYFLLIPAMVPMAIATQSV
                     VGEKNARSLEPQLATPMEVGELLLGKAIASATPAVLATWGVFALYGLVNGWVADPVLT
                     RLIFNDVWRVAMLTLVPLICLLSVLLGIVVSSRVNDARTAQQIGGFIVIPVIGVAIAG
                     FFSGQASFSMEQVLIGDLVVAALIGLTLAVGNWIFDREAILTRLG"
     gene            complement(4726..5409)
                     /locus_tag="VFV59_03625"
     CDS             complement(4726..5409)
                     /locus_tag="VFV59_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324952.1"
                     /translation="MAPEPTRVALEVAPKRSFASALDWPGWARSGRTPDEALHRLLAY
                     APRYAAVLRGTRLGFAAPASVHELEVVERLDGDGGTEFGVPGTAARAESQAMDALALD
                     RSVALLRAAWRAFDRAAAAAEGATLTTGPRGGGRTLDKMRSHVLDAEVAYLGQLGSRP
                     PALEGDAPERMERIRAALIETLTARVHGDELPNPRKTKHPWSAAYAVRRIAWHALDHA
                     WELEDRTPR"
     gene            complement(5414..5830)
                     /locus_tag="VFV59_03630"
     CDS             complement(5414..5830)
                     /locus_tag="VFV59_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5324953.1"
                     /translation="MTVELAQQLASVPLLAGLNDRVRRRLADIGKRRSYNPDDAIVRE
                     GSAGTALYIVLSGRARVERAGEVLGELKAGDFFGELALIEEHPRSASVIAAEPTDCLL
                     FPAWEFTALLEEHPEVAVPIMRVLIARLHRREHHKA"
     gene            complement(5889..8696)
                     /locus_tag="VFV59_03635"
     CDS             complement(5889..8696)
                     /locus_tag="VFV59_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU5324954.1"
                     /translation="MTTTRTPIHLVPHTHWDREWYRPFQSFRMQLVDLVDRVLEMLEA
                     DPDFAFTLDGQLATIDDYLEIRPEQAERIRRHVQDGRLAIGPWQILMDEFLVSGETLV
                     RNLEMGWRRAEDFGDPMRAGYLPDMFGHVAQMPQLLRRAGLADAIVWRGVPAVIDRHR
                     FRWESPDGSWVRAEYLPAGYGSAAYLFAVPDRLDAAAARFVDWARPWFGSDPILAMYG
                     TDHTAPVPELVALVGRLNEVHDASRMRISTLAQYLDAAAPLQEADPCWRGEMRSGARA
                     NVLMGVASARIDIKQAAGRAETLLERYAEPLSALHVTPAAWPGAFLELAWRRVIENSA
                     HDSICGCSVDPVVDQVLVRFAEAEQIGAALGRRAGVAVASVVPRGAVTVLNPSAAPRE
                     GLVEAELQVPDEWADIALELPDGARIGTQELSRKEPVLFDRVLRGDQVDELFRRFHGR
                     EVFDHSWNGYRLDGRTITLEVDDDPDPVWLDVDGLRAEVVAAMRSAPADEWQIRIVAR
                     PRRRIVASVPAPALGWTAVRPVDAAAPVADGVALGDDGRSLDNGLLRVEVADDGTFSV
                     RAAGASAAGLGRIVDGGDFGDSYNYGPPADDSLIEVAFRTVIEPLLGGPVVARLAVDR
                     TYAWPGGVTPDGARRTARTEETVVRTVLELRAGEPFVRVRVEFENRSDDHRVRFHAPL
                     PRPADTSSAEGQFAVVERGVVGEGGYREEPLATYPAHGWVDAGGLAILLDHLAEYELT
                     DDGRELAITLLRSTGLISRNDNPYRQDPAGPEIAIPNAQMRGRRSYDFAIFPHAGNWD
                     AGGVADAAERYRHAFVTAPGSADRGAPWPPQGAGGDALEVRGANVVLSSLRRRDGGWL
                     EARLVNLTTEPATSVVSAAITEAREATLRGEPRGDLDVSDGAVTLRLGPAEIRTIQFR
                     RSETAELRPEVLDASGPRQSG"
     gene            complement(8759..9574)
                     /locus_tag="VFV59_03640"
     CDS             complement(8759..9574)
                     /locus_tag="VFV59_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU5324955.1"
                     /translation="MATLRVVSWNIRAAIGTGPFPDRWWRRIDADRLRAIGSFVASQE
                     PDVVALQEAALVSRNGDLVDNAGDLARELGMAVRYGAVRTFAVTDETDATVGVGCFGN
                     ALLSRAPLDEVRCVALPAAPMDAFVEPPGADHPAAGVRYADAPPTIREPRCLLLARVG
                     ALTIGTTHYSHVGSGERLLQAEATVRAFGDTAPAVLLGDLNAAVDAAELRPFESWTDG
                     FVVPVGDHARISTDDGYRIDQLFARGASVAECRVLPESGDLSDHYPVVATIEW"
     gene            complement(9577..10482)
                     /locus_tag="VFV59_03645"
     CDS             complement(9577..10482)
                     /locus_tag="VFV59_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012307645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5324956.1"
                     /translation="MTAAVGDQTVRAPARTLSEKLSRFVGRAPVHIALAIVALIWLLP
                     TIGLFITSWRPRSEIQNTGWWTIFTGNLSFTADNYSQVIGAQDMGTAFLNSVWITVPA
                     TLIPVFLCAFSAFAFAWMRFPLRDTLFLGVVAMLLIPIQMVLIPLSRLFGDIGLAGNS
                     GFLGFVPVWIVHITFALPFGIFLLRNFFITLPKDLIEAARLDGASNWQIFREVVVPLS
                     VPALASYGIFQFLWVWNDLLTALVFAPRGGKPMTVQVTSLLGTYGSEWNLLAAAAFLV
                     MAVPLIVFFSLQRYFVQGLLAGSVK"
     gene            complement(10479..11402)
                     /locus_tag="VFV59_03650"
     CDS             complement(10479..11402)
                     /locus_tag="VFV59_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5324957.1"
                     /translation="MTAATGAQTTTPRSSGPSWLRGWSWQASLFLAPALFLIAVFMLY
                     PTISTIILSFENGLNNYIRLFTGDPRFLNLSTFPPSGALFNNVLWVIFYTGGCIILGL
                     VIAVMATRVRYEALVKAIVFIPQAIAATALGVIWRFVYFPDADTGLLNAGLGVFGIDP
                     IAWLGSTSWVNTALIIAGIWGSVGFATVILSAALKGISTEILEAARVDGARETQIFWR
                     IIVPMLSLPISVVAVTLIINVIKLFDLIYAMTQGGPANSSRVIAFSMYQEALPSRNFD
                     YGAAIAVVMLLILLPVMAYNVRRFRAEKVVQ"
     gene            complement(11479..12843)
                     /locus_tag="VFV59_03655"
     CDS             complement(11479..12843)
                     /locus_tag="VFV59_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324958.1"
                     /translation="MKKFRFVAPVIAGMLVLAACTSDDGGSSAGASGGASGGGGASGG
                     GTGNLEQGDGSIEVTSLWGGAEAEAFQAVLDAFTEASGIEVEYTSVRQDYATVLNNRL
                     AQSDPPDVAIIPGIGFLRSFAKDDLLIPLSDLGVDESYLSSAYAENLADMGQAVGSVN
                     GTPYAVMVKLNSKATVWYKPDSFDENSWTVPESWDDMVSLTGQIKDDGSTPWAVGASD
                     SWTLTDWFEMVYLTQAGPEAYDTLFSADGNWTDQTVLDAAAAMTEIINDDNVVGGLDG
                     ALATGFVDGIGQVFSENADAQLYYEGGFVGGIALGDVNPDLEIGTGIDFFDFPGAMDQ
                     GITVGGDVMAAFVADSDVAEFMQYLASTEGGTKWAEGGTIISPMAGVETSVYESVNQL
                     VVQEAEQIANAPTARFDGSDLLPAGTDLGAVLQQVIQNPDNAQSAFEQFQSEVDAAWA
                     DLEG"
     gene            complement(12966..14057)
                     /gene="ugpC"
                     /locus_tag="VFV59_03660"
     CDS             complement(12966..14057)
                     /gene="ugpC"
                     /locus_tag="VFV59_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU5324959.1"
                     /translation="MATVTFDHMYKRYGEDVIAVNDLNLEIGDGEFICLVGPSGCGKT
                     TALRCVAGLEEITDGRLLIGDRVVNNVAPKDRDIAMVFQSYALYPHMSVFDNLAFGLK
                     LRKVKKDEIDKRVKEAAGILGLERFLDRKPKALSGGQRQRVALGRAIVREPSVFLMDE
                     PLSNLDAKLRVQTRAEIARLHQRLGTTTIYVTHDQVEAMTMGDRIAVMRDGLLQQVGT
                     PQELYDHPSNVFVAGFIGSPAMNFATVKATDGGDLQLGGAQLRLAGEPARAAEQQPKG
                     KDLLIGFRPEHLVLANGGDGLVRIPAKVDVVEYLGNDELIHAQAEGHEVVALISSDER
                     VKVGDSVDFGIPMDKLHIFDPESEKTLAG"
     gene            14181..14828
                     /locus_tag="VFV59_03665"
     CDS             14181..14828
                     /locus_tag="VFV59_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324960.1"
                     /translation="MSTLFSLARWLVRAMGRALLDGLRLALHATGQIRAGAAERRNAV
                     RNVLPSLYDRHPHARTAPRRPVGVRPVELDRIVGTARSPSQNTADFLPLPGLRGQNWR
                     ARWQRINRAMDRLSVLPPIELVQVGDEYFVVDGHNRVAAALRAGAVEIDADVVQLLVP
                     GVRPSGGAVLDSTALIGTESVRQAGQGRYSRTVEQRLPQDRVTREQLLAADEDRE"
     gene            14825..15517
                     /locus_tag="VFV59_03670"
     CDS             14825..15517
                     /locus_tag="VFV59_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5324961.1"
                     /translation="MTDRLRVVWPDPKPFAARDGEPIRILAISDEPDPSLDSEATREG
                     LGRVDLIVGAGDLQPDYLSFVSDAFHAPLRYVRGNHDVGLAWSHTRRKLLPDPMPDGE
                     LIHELGIRILGFSGSPRYNQRGMQVSSLGMWWQVLTAWFRARSARPLLMVTHAPPRGL
                     NDDRDLAHRGFKAFRWLIDRLAPPLWLHGHTALVRRGLDDRCEQHGPTLLYNCTGATL
                     VELLPPDADARG"
     gene            15510..15992
                     /locus_tag="VFV59_03675"
     CDS             15510..15992
                     /locus_tag="VFV59_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324962.1"
                     /translation="MDEPVPVGRAVAGCLVIAIAGFVLALLVRPLIFTVAPPRDDSAV
                     SVAAAGDVRGAPVERDVLLSRSYGWDGERDAGDGRVQLRLIVAPVRFGGVAVLNGSSP
                     AAEDCRVEIGADRLTDCEGRAWTFEGDPLDSADPPLQRFPASSESGTIVADLTRTLDR
                     "
     gene            16068..16337
                     /locus_tag="VFV59_03680"
     CDS             16068..16337
                     /locus_tag="VFV59_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5324963.1"
                     /translation="MGDLGFLGWIIIGLLAGAIAGFFVPGRERFGCLGTMLIGVVGGF
                     LGGFLWVNVLGQDEASGWLGALVIAVIGSIIVLLVLRAMRGGDRT"
     gene            16420..16495
                     /locus_tag="VFV59_03685"
     tRNA            16420..16495
                     /locus_tag="VFV59_03685"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16453..16455,aa:His,seq:gtg)
BASE COUNT         2397 a         5749 c         5879 g         2470 t
ORIGIN      
        1 cgcatccgag cggggcaacg cgcggcacgc cgggttcaga ggaggaccaa gccgttcgca
       61 ccggggtcga gcccggcact gagcgccgca agcgttgttc gcgtatcaag gggaaagctc
      121 tgccgcaggg cgccgatgtg attgcgaacc agggttcggt tggcatgggt cgcggcaagc
      181 agcaggatcg tgcgctcgac gccgctgtcg cgctgcttcg cactgatcga gcgctgcgtg
      241 gcctcgaggt tcgcgggtcg tgtctcagcc tcgacgccga tccggacgag gcccgagagc
      301 acttcatccc acgcccgcag gtctccgcgg atcggcatca cgacctcgaa cgtgcgatgc
      361 cacgcggggg acacgcgcgc attgaaccgt gccagcagcc gcgcctggcc ggcatcccgc
      421 aggggctcgc cgacggggta catcttcacc gacagcttgt gaccgaggca ggcgaacagc
      481 cgagcgctcc gtgccaggtc gacagagccc aacctgccca gctccagtcg cgccacccag
      541 ccgcgcgata cgccgatggt ccgcgccagc tcggcctggg agatgccggc tcgcatgcgg
      601 agcgtcctcg cctcgcgacc cagttgcgag cgcaggtagc tggcctgctg aatcccgatg
      661 gcgcggatcc gttccctgct cggcatgggt acgagtctgc gcgtggatcc cgtaccagct
      721 gcttaccagc cggttaccgc gtaggtgcga gtggacacag cgaccttcct gatgcacccc
      781 agcgggggct gcacgacctc cgagtggctc gggcagcacc cgacacaggg cggtcggcag
      841 gtgacgggtc catacgcacc cgtgtgggga ctggcaaccg ctgcgggagg cggtcccgct
      901 ggtccgcgac gcccggccga acgtacccgt gggcggaggc ggggattccg ggataggatg
      961 cgaacgaccc catgaacgaa gcgatcagca acatctggcg ccgtccgctg cggacggcgc
     1021 tcaccatcct cggcatcgtc atcggcgtct tcgcgctcac ggtgatgggt gcgctggccg
     1081 agaagctgaa cgtcctcgtc gacggcgggg acgagtactt ccgcacgcgc ctcttcgtct
     1141 tcgacgaggg cgccgcctcc ggcttccagg ccagctcccg gccgatcacg cgcgagctgg
     1201 tcgaccagat cgagcagatc gacggtgtcg agcgggcgac gccggtgctc cagaccctgc
     1261 tggacccgga cgaggtcggc ggcttcgaca tccccgagct gctgaccggc atcgagccgg
     1321 agacgtggta cgacgacagc gtcgtcctgg gtgtcgacgc gggccgcctg ctggaggcgg
     1381 gcgacgaggg cgtgaccacc atcggcgccg atctggcgga gcgctacgac ctccgggtcg
     1441 gcgacacgtt caccatccgt gagcgcgatt tcgaggtcat cggcgtgctg gagcggaccc
     1501 tgaccttccc ggacaagatt gcctacgtcc cgctggccga tgcgcagcag atctacgtcc
     1561 aggacgtccc cgagggtttc gaggagcgga ccggggacct cgccacgcag atcgaggtct
     1621 tcccgaacga cctctcggag gccgatgcga tcgccgaggc gatcgacgag caggtcgacg
     1681 gcgtccgcgc catcaccccg cagcaggtgg ccgaccagat cggccaggcg agcatcgtgt
     1741 tcaacctgat catcgtcggg gccagcgtca tcgccgtgat cgtcggcggc ctcagcgtga
     1801 tcaacaccat ggtgatgacc gtcagcgagc gggttcgcga gatcggcatc aagaaggcgg
     1861 tcggcgcccc gacctccgcc atcctgcgcg agttcgtggt cgaggcgacc ctgctcgggg
     1921 caatcggcgg cctcatcggc ctgggagcgg gcgcacttct ggtcgtcatc ctcaatgcgc
     1981 agacggccgg atccgggacg acggtcttcc tgttgacccc gatgctgctc ctgcgctcgt
     2041 tcctgttcgc gaccatcctg ggcgcactgg ccggcatcta cccggcgctg cgggccgcca
     2101 ggttggatcc cgttcgcgcc ctgcggagca cctgagcgga tgcccatgcc cgagccgatc
     2161 ctcagcgcgc ggcagctgac caaggtctac cggcgcggtg cctcggaggt gcgcgcggtc
     2221 gatggcgtcg atctggacgt tgcaccgggt gagttcatgg cgatcatggg tcggtcgggg
     2281 agtgggaaga cgacgctgct cgatctgctg ggctgcctgc tgcgcccgac ctccggggag
     2341 ctgacgatcg acggacggag cgtcatcggc gcctccgacg gcgagctggc ggcgctgcgg
     2401 cggcagcgca tcggcttcgt cttccaggag ttcaacctga tcccgacgct gaccgccgtc
     2461 gagaacgtcc tgcttccgct ccgatatggc ccgcgccggc ccgacggacg acggcgagcc
     2521 tccgagctgc tcgacctggt ggggctggcg gagcgggcga cgcaccggcc gacggagctg
     2581 tcgggcggcg agcagcagcg cgtggcgatc gcgcgggccc tggtgaacga gccggccgtg
     2641 atcctcgccg atgagcccac gggcgagctc gactcggcca cctcggagcg gctgatggcg
     2701 acgctgcgtg agttgaaccg ggagcgcggc gtgacgacga tcatcgtgac ccacgacgcg
     2761 ggcgtggccg gcgccaccga ccgggtcgtg cgcctccagg atggccatgt cctggccgat
     2821 gcgcgccgca acggcggcaa tggccgcgcc ttcgacgccg cgctgaccgg gcagccgcac
     2881 accgacgggg gcggccgcgt ctgggcgcgg ctcggcgacc tgctggaccg ggcgcgcggc
     2941 cgatgatcga ggcgatcggg ctcgccaagc agttccggac gctcacggcc gtccacgacc
     3001 tctccttcgc cgtcggggat ggcgagatct tcggcctgct ggggccgaac ggtgcgggca
     3061 agacgacctc gatccgcatg ctcgcgggcc tcatcgcgcc gagcgcgggc acggcccgcg
     3121 tcaacggcct ggagctcggg ccccagtcac agcgcatccg cgccctcacc ggcgtactga
     3181 ccgagtcgcc cggcctgcac gaccggctga ccgcgcgcca gaacctcggc tattacggcc
     3241 gcctctacgg cctgcggggc ggcgagctgc gtcgggcggt cgaccgctac ctgggaatcg
     3301 tgggactgac gccggcggcc gaccgtcggg tcggcggctt cagcaaaggc atgcgccaga
     3361 aggtcgccat cgcccgggcg ctgctgcacg agccgcaggt gctctacctg gacgagccga
     3421 ccagcgccct ggacccatcg gccgccaagg gcgtccgcga cttcgtcgcc acgctccgcg
     3481 acgccggccg ctcgatcgtg gtctgcacac acaacctgga tgaggcggag cggctgtgcg
     3541 accgcatcgg catcatgcgt ggcacgctgc tgacggtcga cacgccggcg cgcctgcgcc
     3601 ggcgcaacgg caccgcgacc gtgcgggtcg agctggtcgg cgcgcggcgg cccacctcgt
     3661 tcctcgacct gctgggccgg ctcgcgttcg tggagggcgc tcgggccgtc gacggggcgc
     3721 tgctggtcga ggttcgcgag ccgcgcgggg acaaccccga gctcgtgcgc gagctcgtcg
     3781 aggccggcgc gcgcatcgtg cacgtcagcg aggaggcggc gacgctcgag caggtctacc
     3841 tcgacctcgt gggcgaggcg ggagcgcgcg atgccggcgc gggcatcggc ctggactccg
     3901 acggggaggc cggctgatgc gacccggcta cgtgtgggcg gtcatcggca aggaagcccg
     3961 cgacctgctg tcgaaccggc tgctgctggg ggccgtcgtg ttcccggccc tcgtcttcgc
     4021 cgccatcccg accgggatcg tggcgttcat cgagttcaac gagctggatc cggatcagct
     4081 ggggcagatc gagcagtaca tcagccagtt ccccgacctg ccggcgaagc tggcggccca
     4141 ggggttcatc gtcatgaact tcatggccta cttcctcctc atcccggcca tggtgccgat
     4201 ggcgatcgcc acgcaatcgg tcgtcggcga gaagaacgcg cgctcgctcg agccgcagct
     4261 ggcgacgccg atggaggtgg gggagctgct gctcggcaag gccatcgcct ccgccacgcc
     4321 ggccgtgctg gccacctggg gcgtctttgc gctctacgga ctggtgaacg gctgggtcgc
     4381 cgatccggta ctgacccgcc tcatcttcaa cgacgtctgg cgcgtcgcga tgctgaccct
     4441 cgtgcccctg atctgcctgc tcagcgtgct gctggggatc gtcgtcagct cgcgcgtcaa
     4501 cgacgcccgg acggcacagc agatcggcgg cttcatcgtc atccccgtca tcggcgtggc
     4561 gatcgccggc ttcttcagcg gccaggcgtc gttcagcatg gagcaggtgc tcatcggcga
     4621 cctggtcgtc gcggcgctga tcgggttgac gctggcggtc ggcaactgga tcttcgaccg
     4681 ggaggcgatc ctcacgcggc tcgggtagcg aacgctcccg gcgaatcagc gcggcgtgcg
     4741 gtcctccagc tcccacgcat ggtccagcgc gtgccacgcg atccgccgaa cggcgtaggc
     4801 ggccgaccac gggtgcttcg tcttgcgtgg attcggcagc tcgtcgccgt ggactcgcgc
     4861 ggtcagggtc tcgatcagcg ccgcacggat gcgctccatg cgctccggcg cgtccccctc
     4921 cagcgcgggc ggccgagatc cgagctggcc gaggtacgcg acctcggcat ccaggacgtg
     4981 cgagcgcatc ttgtccagcg tgcgcccgcc gccccgtggc cccgtcgtga gggtcgcgcc
     5041 ctccgcggcc gccgcggcac gatcgaacgc gcgccaggcg gcgcggagca gggcgaccga
     5101 tcggtccagt gccaaggcgt ccatcgcctg cgactcggcg cgcgcggcag tgccgggtac
     5161 gccgaactcg gtgccgccgt caccgtcgag ccgctccacg acctcgagct cgtggacgga
     5221 ggccggcgcc gcgaagccga gcctggtgcc gcgcagcacg gcggcatagc gcggggcata
     5281 cgcgagcaat cggtgcagtg cctcgtccgg cgtcctgccg gaccgcgccc agcctggcca
     5341 gtcgagcgca ctggcaaagc tgcgcttcgg ggcgacctcg agtgcgacgc gcgtcggttc
     5401 cggggccaag gacctacgcc ttgtggtgct cccgccggtg cagccgggcg atcaggaccc
     5461 gcatgatcgg caccgccacc tcgggatgct cctcgagcag ggccgtgaac tcccacgcgg
     5521 gaaagaggag gcagtcggtc ggctcggccg cgatcacgct ggcggagcgc ggatgctcct
     5581 cgatcagggc cagctcgccg aagaagtccc cggccttgag ctcgccgagc acctcacccg
     5641 ctcgctcgac gcgggcgcgt ccgctgagca cgatgtagag cgcggttccg gcggatccct
     5701 cgcggacgat cgcgtcgtcg gggttgtagc tgcgccgctt cccgatgtcg gcgagccggc
     5761 gccggacgcg gtcgttcagg cccgccagga gcggcaccga tgcgagttgc tgcgcgagct
     5821 ccacggtcat cctccgttct cttccgccga tgcgcgatgc ggggcgtggc gcgtatccta
     5881 gcgcgccctc atccgctctg acgcggcccg ctggcgtcca ggacctccgg acgcagctcc
     5941 gcggtctcgg agcggcggaa ctggatcgtg cggatctccg ccgggcccag ccgcaacgtg
     6001 accgcaccgt cggaaacgtc caggtcgccg cgcggctctc cacggagcgt cgcctcgcgc
     6061 gcttccgtga ttgcggcgct cacgacggac gtcgcgggct ccgtcgtgag gttcaccagg
     6121 cgcgcctcca gccagccgcc gtcacgccgg cggagcgagc tcagcacgac gttggcgccg
     6181 cgcacctcga gggcgtcacc gccggcgccc tgcggcggcc acggggcgcc gcggtcggca
     6241 ctgcccggag ccgtgacgaa cgcatggcgg taccgctcgg ccgcatcggc gaccccgccg
     6301 gcatcccagt tcccggcgtg cgggaagatg gcgaagtcgt agctccgtcg gccgcgcatc
     6361 tgcgcgttcg gaatcgcgat ctcggggccg gccgggtcct ggcggtacgg gttgtcgttg
     6421 cggctgatga gcccggtcga tcggagcagg gtgatggcca gctctcggcc gtcgtcggtg
     6481 agctcgtact cggcgaggtg gtcgagcagg atggccagcc ccccggcgtc gacccagccg
     6541 tgcgccgggt acgtggccag cggctcctcc cggtagccgc cctcgccgac cacgccgcgc
     6601 tcgacgacgg cgaactgccc ctcggcgctg gaggtgtcgg ccggccgcgg caggggagcg
     6661 tggaagcgca cgcggtggtc gtcggagcgg ttctcgaact cgacccggac gcgcacgaac
     6721 ggctccccgg cgcgcagctc gaggaccgtg cggaccacgg tctcctcggt gcgcgcggtg
     6781 cgacgcgcac cgtcgggcgt cacgccgccc ggccacgcgt acgtgcggtc gacggccaac
     6841 cgtgcgacga ccgggccgcc gagcagcggt tcgatgaccg tcctgaacgc cacctcgatg
     6901 agcgagtcgt cggccggagg gccgtagttg taggagtcgc cgaagtcgcc gccatcgacg
     6961 atccggccca ggcccgcagc cgatgcgccg gccgcgcgga ccgagaacgt gccgtcgtcg
     7021 gcaacctcga cgcgcagcag gccgttgtcg agggatcggc cgtcgtcgcc cagggcaacg
     7081 ccgtcggcga ccggcgccgc ggcgtcgacg ggccggaccg ccgtccagcc gagcgccggg
     7141 gccgggacgg acgccacgat gcgccgtcgg ggccgcgcca cgatccggat ctgccactcg
     7201 tccgccgggg cggaccgcat ggcagccacc acctcggcgc gcagcccgtc gacatcgagc
     7261 cagacggggt ccgggtcgtc gtcgacctcg agggtgatgg tgcgaccgtc gaggcggtag
     7321 ccgttccacg agtgatcgaa gacctctcgg ccgtggaacc gtcggaagag ctcgtcgacc
     7381 tggtcgccgc gcaggacgcg atcgaagagg acgggctcct tgcgcgacag ctcctgcgtg
     7441 ccgatccgtg ctccgtcggg gagctcgagg gcgatgtccg cccactcgtc gggcacctgc
     7501 agctcggcct cgaccaggcc ttcgcggggc gcggctgacg ggttcaggac ggtcaccgca
     7561 ccgcgtggca ccaccgaggc gaccgcgacg cctgcgcggc ggcccagcgc ggcgccgatc
     7621 tgctcagctt cggcgaatcg gaccagcacc tggtcgacca ccgggtcgac ggagcacccg
     7681 cagatcgaat cgtgcgcgga gttctcgatc acccgtcgcc aggcgagctc caggaacgcc
     7741 ccgggccatg cggcgggggt gacgtgcagg gcgctcagcg gctcggcgta gcgctccagg
     7801 agcgtctcgg cgcgtccggc cgcctgcttg atgtcgatcc gcgcgctggc gacgcccatc
     7861 aggacgttcg cgcgcgcccc ggaacgcatc tcgccgcgcc agcacggatc cgcctcctgc
     7921 agcggcgcgg cggcatccag gtactgggcc agcgtgctga tccgcatccg ggaggcgtcg
     7981 tggacctcgt tcagccgccc gaccagcgcc accagctccg gcacgggtgc ggtgtgatcg
     8041 gtgccgtaca tcgcgaggat cgggtccgat ccgaaccagg gccgtgccca gtccacgaag
     8101 cgggccgccg cggcatccag ccggtccgga acggcgaaga ggtatgccgc actgccgtag
     8161 ccggccggaa ggtactcggc gcggacccac gagccatccg gcgactccca gcggaaccga
     8221 tggcggtcga tcaccgcggg gacgccgcgc cacacgatcg catcggccag gccggcgcgt
     8281 cgcaggagct gcggcatctg ggccacgtgc ccgaacatgt cgggcaggta accggcgcgc
     8341 atggggtcgc cgaaatcctc ggcgcgacgc cagcccatct ccaggttccg cacgagcgtc
     8401 tcgccgctga ccaggaattc gtccatcagg atctgccacg gaccgatggc gaggcggccg
     8461 tcctgcacgt ggcgccgaat gcgctccgcc tgctcgggtc ggatctcgag gtagtcgtcg
     8521 atcgtcgcca gctgcccatc gagggtgaag gcgaagtcgg gatcggcctc cagcatctcc
     8581 agcacgcggt cgacgaggtc gacgagctgc atccggaacg actggaaggg ccggtaccac
     8641 tctcggtccc agtgcgtgtg cgggacgagg tggatgggcg tgcgcgtggt cgtcatctgt
     8701 ccggcccgac cttaccggct cgagccgtcg ggcgccgtac gacgaatgac gtacgttctc
     8761 accactcgat cgtcgccacg accgggtagt ggtccgacag gtcgcccgac tcgggcagta
     8821 cccgacactc ggcgacggat gcgccacgcg cgaacagctg gtcgatgcgg tacccgtcgt
     8881 cggtcgagat ccgcgcgtgg tcgccgacgg ggaccacgaa tccgtccgtc cacgattcga
     8941 agggccgcag ctcggccgcg tcgacggccg cgttgagatc gcccagcagc accgccggtg
     9001 ccgtgtcacc gaacgcccgg acggtcgcct cggcctggag cagccgctcc ccggatccga
     9061 cgtggctgta gtgggtcgtt ccgatcgtca gcgctccgac gcgggccagc aacaggcagc
     9121 gcggctcgcg gatggtgggc ggcgcatcgg cgtagcggac gccggcggcc ggatggtcgg
     9181 cgccgggcgg ctccacgaag gcgtccatcg gcgcggcggg aagggcgacg catcggacct
     9241 cgtccagcgg cgcacgcgac aggagcgcgt tcccgaagca gccgacgccg acggtcgcat
     9301 cggtctcatc ggtgacggcg aaggtgcgca cggcgccgta gcggacggcc atcccgagct
     9361 cacgcgccag gtctccggcg ttgtcgacca ggtcgccgtt gcgcgagacg agcgcggcct
     9421 cctggagcgc gacgacgtcg ggctcctggg aggccacgaa cgagccgatc gcccggagcc
     9481 gatcggcgtc gatgcggcgc caccatcggt ccgggaatgg cccggtcccg atggcggccc
     9541 ggatgttcca actgacgacg cgcagcgtcg ccaccgctac ttgacggaac ccgcgagcag
     9601 gccctggacg aagtagcgct ggaggctgaa gaagacgatg agcggcacgg ccatgaccag
     9661 gaaggccgcc gcggccagga ggttccactc cgagccatag gtgccgagca gggaggtgac
     9721 ctgcaccgtc atcggcttgc cgccgcgcgg ggcgaacacc agggcggtca gcaggtcgtt
     9781 ccagacccag aggaactgga agattccgta gctcgccagc gcggggacgc tcaacgggac
     9841 caccacctcg cggaagatct gccagttcga cgcgccgtcg agtcgcgcgg cctcgatcag
     9901 gtccttgggc agggtgatga agaagttgcg cagcaggaag atcccgaagg gcagcgcgaa
     9961 ggtgatgtgg acgatccaga ccggcacgaa gccgaggaac ccgctgttcc cggccaggcc
    10021 gatgtcaccg aagaggcggc tcagcgggat gaggaccatc tggatcggga tgaggagcat
    10081 cgcgacgacg ccgaggaaga gcgtgtcgcg cagcgggaac cgcatccagg cgaaggcgaa
    10141 cgcgctgaat gcgcacagga acaccgggat cagcgtcgca ggcacggtga tccacacgga
    10201 gttgaggaac gccgtgccca tgtcctgggc gccgatgacc tgcgagtagt tgtcggcggt
    10261 gaagctcagg ttcccggtga agatcgtcca ccagccggtg ttctggatct cggaccgcgg
    10321 tcgccaggac gtgatgaaca ggccgatggt gggcagcagc cagatgaggg ccacgatcgc
    10381 cagggcgatg tggaccgggg cgcggccgac gaaccgggac agcttctccg agagggtacg
    10441 cgcgggcgcg cggaccgtct ggtctccgac ggctgcggtc attgcaccac cttctctgcg
    10501 cggaagcgac ggacgttata ggccatgacc ggcagcagga tcaggagcat gaccacggcg
    10561 atggccgcgc cgtagtcgaa gttccgtgac gggagggcct cctggtacat gctgaacgcg
    10621 atcacgcggc tcgagttggc cggtccgccc tgcgtcatgg cgtagatcag gtcgaagagc
    10681 ttgatcacgt tgatgatcag cgtcaccgcc acgacgctga tgggcagcga gagcatcggc
    10741 acgatgatgc gccagaagat ctgcgtctcc cgcgcgccgt ccacgcgcgc cgcctccagg
    10801 atctcggtgc tgatgccctt gagcgcggcg gagaggatga cggtcgcgaa gccgaccgag
    10861 ccccagatgc ccgcgatgat tagcgccgtg ttgacccagg acgtgctgcc gagccaggcg
    10921 atggggtcga tgccgaagac gcccaggccg gcgttgagca ggccggtgtc cgcgtcgggg
    10981 aagtagacga agcgccagat gacgccgagg gccgtggccg cgatggcctg cgggatgaag
    11041 acgatcgcct tgaccagcgc ctcgtaccgg acccgcgtcg ccatgaccgc gatcaccagg
    11101 cccaggatga tgcagccgcc ggtgtagaag atgacccaca gcacgttgtt gaagagcgcg
    11161 cccgacggcg ggaacgtgct gaggttgagg aagcgtgggt cgccggtgaa caagcggatg
    11221 tagttgttca gcccgttctc gaacgacagg atgatcgtgc tgatcgtcgg gtagagcatg
    11281 aacaccgcga tcaggaacag ggcgggcgcc aggaacagac tcgcctgcca gctccatccg
    11341 cgaagccagc tcggcccgct cgatcggggc gtggtggtct gcgcgccggt tgctgccgtc
    11401 atcgcaccct ccagtgtggg aaatgctcag gggcggccgc gcggccgccc ctgagacatc
    11461 gaacgatctt cggccggact acccctcgag gtcagcccag gccgcgtcga cctccgactg
    11521 gaactgctcg aacgcgctct gcgcgttgtc cgggttctgg atgacctgct ggagcacggc
    11581 gcccaggtcg gtgcccgccg gcaggaggtc cgagccgtcg aaccgcgcgg tcggcgcgtt
    11641 cgcgatctgc tcggcttcct gcaccacgag ctggttgacc gactcgtaga cgctggtctc
    11701 gacaccggcc atcggcgaga tgatcgtccc gccctcggcc cacttggtgc cgccctcggt
    11761 gctggccagg tactgcatga actcggcgac gtcgctgtcg gccacgaagg ccgccatcac
    11821 gtcaccaccg accgtgatgc cctggtccat ggcgccgggg aagtcgaaga agtcgattcc
    11881 ggtgccgatc tcgaggtccg ggttgacgtc gccgagcgcg atgccgccga cgaatccgcc
    11941 ctcgtagtac agctgggcat cggcgttctc gctgaagacc tggccgatgc cgtcgacgaa
    12001 gcccgtcgcc agcgcgccgt cgaggccgcc gaccacgttg tcgtcgttga tgatctcggt
    12061 catcgccgcc gcggcgtcga ggaccgtctg atcggtccag ttgccgtcgg cgctgaacag
    12121 cgtgtcgtac gcctcagggc cggcctgggt caggtagacc atctcgaacc agtcggtcag
    12181 cgtccacgag tcggacgcgc cgaccgccca cggcgtgctg ccgtcgtcct tgatctggcc
    12241 ggtcaggctg accatgtcgt cccagctctc gggcacggtc cagctgttct cgtcgaacga
    12301 gtccggcttg taccagacgg tggccttgct gttgagcttg accatgacgg cgtacggcgt
    12361 gccgttgacg ctgccgaccg cctggcccat gtcggccagg ttctcggcgt aggcgctcga
    12421 gaggtagctc tcgtcgacgc ccaggtcgga cagcggaatg agcaggtcat ccttcgcgaa
    12481 gctgcgcagg aagccgatgc ccggaatgat cgccacgtcg ggcggatcgc tctgcgcgag
    12541 gcggttgttg aggaccgtcg cgtagtcctg ccggaccgag gtgtactcga cctcgatgcc
    12601 gctggcctcc gtgaaggcgt cgagcacggc ctggaaggcc tcggcctccg cgccgcccca
    12661 cagcgaggtg acctcgatgc tgccgtcacc ctgctcgagg ttgcccgtgc cgccgccact
    12721 ggcgccgcca ccgccgctgg cgccaccact ggctccagcg ctcgagccgc catcgtcgga
    12781 ggtgcacgcc gcgaggacga gcatgccggc gatgaccggg gctacgaacc gaaacttctt
    12841 catccgattc ctccaccatt tccgttgtcc gcggcacggc cgcggctcgc atcgggccgc
    12901 accggttggt cccggcgcgc ccgcccagat cggtgtccct ccaggtgtct gcggcctccg
    12961 tccgctcagc cggccagcgt cttctccgac tcggggtcga agatgtgcag cttgtccatg
    13021 gggatgccga agtcgacgct gtcgccgacc ttgacgcgct cgtcggagct gatcagggcg
    13081 accacttcgt ggccctcggc ctgtgcgtgg atgagctcgt cgttgccgag gtactccacg
    13141 acgtcgacct tggccggaat gcgcaccagc ccgtcgccgc cgttggccag gaccaggtgc
    13201 tcgggtcgga agccgatgag cagatccttg cccttgggct gctgctcggc ggccctggcg
    13261 ggctcgccgg ccaggcgcag ctgcgcgccg cccagctgga ggtcgccccc gtcggtcgcc
    13321 ttcaccgtcg cgaagttcat ggccggtgag ccgatgaacc cggcgacgaa gacgttcgac
    13381 ggatggtcgt acagctcctg cggtgtgccg acctgctgga gcaggccgtc gcgcatgacg
    13441 gcgatccggt cgcccatggt catggcctcg acctggtcgt gggtgacgta gatggtcgtg
    13501 gtcccgaggc gctggtgcag ccgcgcgatc tcggcgcgcg tctgcacgcg cagcttggcg
    13561 tcgaggttgg acagcggctc gtccatcagg aacacggacg gctcgcggac gatggcgcga
    13621 ccgagcgcca cgcgctgtcg ctgcccgccg gagagcgcct tcggcttgcg gtccaggaag
    13681 cgctccaggc ccaggatgcc ggcggcctcc ttcacgcgct tgtcgatctc gtccttcttg
    13741 accttgcgca gcttcaggcc gaacgccagg ttgtcgaaga ccgacatgtg cgggtacagg
    13801 gcgtaggact ggaacaccat ggcgatgtcg cggtccttcg gtgccacgtt gttgacgact
    13861 ctgtccccga tgaggaggcg accgtcggtg atctcctcga gtccggccac gcagcgcagg
    13921 gcggtcgtct tgccgcatcc cgatgggccg accaggcaga tgaactctcc gtcgccgatc
    13981 tccaggttga ggtcgttgac ggcgatgacg tcctcgccat agcgcttgta catgtggtcg
    14041 aacgtgacgg tggccatcga gatcctcgct cctgttgtca cggaatccga taggagtggg
    14101 cgcggcggcc cagcctcggg actggcatca tagtacgggg cgcaaccacg ctttccgcag
    14161 ccttcgcgca cctggagacc atgtccaccc tcttctccct cgcccgctgg ctggtccgcg
    14221 cgatgggccg cgcgctcctc gacgggctgc ggctcgcact gcatgcaacg ggccagattc
    14281 gagccggcgc cgccgagcgg cgcaacgcgg tgcgcaacgt cctgccttcc ctgtacgacc
    14341 ggcaccctca cgcgcgcacc gcgccgcgac ggccggtcgg ggtgcggccg gtggagctcg
    14401 accgcatcgt gggcacggcg cgcagcccgt cgcagaacac agccgacttc ctgccgttgc
    14461 ccgggctgcg ggggcagaac tggcgcgccc gctggcagcg catcaaccgc gcgatggatc
    14521 ggctctcggt cctcccaccg atcgagctgg tccaggtcgg cgacgagtac ttcgtggtcg
    14581 acgggcacaa ccgcgtggcc gcggccctgc gggccggcgc cgtcgagatc gatgcggacg
    14641 tggtccagct gctcgtcccc ggcgtgcggc cgtccggcgg tgcggtcctc gactcgaccg
    14701 ccctgatcgg caccgaatcc gtgcgccagg cggggcaggg gcgctactcg cgcacggtcg
    14761 agcagcggct gccccaggac cgcgtgacgc gagagcagct gctggcggcc gacgaggacc
    14821 gcgagtgacc gatcgcctgc gcgttgtctg gccggatcca aagcccttcg ccgcccgcga
    14881 cggcgagccg atccggatcc tcgccatctc cgacgagccg gacccgtccc tcgactccga
    14941 ggcgacccgc gagggactgg ggcgggtcga cctcatcgtc ggggccggtg acctccagcc
    15001 ggactacctc agcttcgtgt ccgatgcgtt ccatgcgcca ctgcgctacg tgcgcggcaa
    15061 ccacgacgtc ggcctggcct ggtcgcacac ccgccgcaag ctgctgcccg atccgatgcc
    15121 cgacggcgag ctcatccacg agctggggat ccgcatcctc gggttctccg gctcaccgcg
    15181 ctacaaccag cgcggcatgc aggtctcgtc gctcgggatg tggtggcagg tgctgaccgc
    15241 gtggttccgc gccaggagcg cgcgaccact cctgatggtg acccacgcgc cgccgcgcgg
    15301 cctcaacgac gatcgggacc tggcgcaccg cggcttcaag gcgttccgct ggctgatcga
    15361 ccggctggcc ccgccgctct ggctgcacgg tcataccgcg ctcgtgcggc gagggctcga
    15421 cgaccgatgc gaacagcacg gccccacgct gctctacaac tgcaccggag ccacgctggt
    15481 cgagctcctg ccaccggacg ccgatgcgcg tggatgagcc ggtcccggtc ggacgcgcgg
    15541 tggccggctg cctggtcatc gcgatcgccg gcttcgtgct ggcgctgctg gtgcgtccgc
    15601 tgatcttcac cgtcgcgccg ccgcgcgacg actccgcggt gtcggtggcg gccgccggcg
    15661 acgtgcgagg cgcaccggtc gagcgggacg tgctcctctc gcgctcgtac ggctgggacg
    15721 gcgagcgtga tgcgggcgac ggccgcgtgc agctgcggct catcgtcgcg ccggtccggt
    15781 tcggcggcgt ggccgtgctc aacggcagca gtccggcggc cgaggactgc cgtgtcgaga
    15841 tcggcgccga ccggctcacc gactgcgagg gacgggcatg gacgttcgag ggcgacccgc
    15901 tcgactcggc cgatcctccg ctccagcgct tcccggcgtc gagcgaatcg ggcaccatcg
    15961 tggccgatct gacgcggacc ctagatcgat agggtgcatc actcggtcgg cacgtcacgt
    16021 gcatcggccg agcccgacca tttcgctcga attacggagg acgatccgtg ggagacctgg
    16081 gattcctggg ctggatcatc atcgggctgc tcgccggcgc catcgctggc ttcttcgtgc
    16141 ccggcaggga gcgcttcggg tgcctgggga cgatgctcat cggcgtcgtc ggcggcttcc
    16201 tcggcgggtt cctgtgggtc aacgtgctcg gtcaggacga ggcgtccggc tggctcgggg
    16261 cgctggtgat cgccgtgatc ggctcgatca tcgtgctgct cgtcctgcgt gccatgcgag
    16321 gcggcgaccg gacctagccg atcgctcagc gcgtgttgcg tgggggcgcg ggatcggcga
    16381 cgatatcggc cccgcgctgc gaccacgcgc ggcttcgtgg tggccttagc tcaactggca
    16441 gagcatcgga ttgtggctcc gaaggttacg ggttcaagcc ccgtaggcca cccca
//
LOCUS       DASTYP010000074         4020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_551211, whole genome shotgun sequence.
ACCESSION   DASTYP010000074 DASTYP010000000
VERSION     DASTYP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551211"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VFV59_03690"
     CDS             <1..1025
                     /locus_tag="VFV59_03690"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324964.1"
                     /translation="LCIGGTVVIGIVDIRLGLVAFALANFAYQAALIYYDALLPDVAH
                     PLTRGRLSGVGVALGYCGTLLVGVILLLGVTVENDRSTPATFALVGGLFALFAAPLFL
                     LVREPARGGAAFSAIDAVRSWRQLADTLDHARRAPGLLRFIVARFFYSDPVNTTIAVM
                     SAFAVNAIGFTEGQALQVLLVLTVVAVLASFGWGVLADRWGPRRTLFAVLAAWALALA
                     ILTAFLSTIPFLVAGALAGAGLGGVGVVDRLMLLRLTPPERVGEMFGLYGLVGKFSAV
                     VGPVVYGIIVANLLDSLDRGAYQVAVGSLLVLLLVGIAILRGVPSGDEENELAAAEVL
                     LEETDR"
     gene            1022..1774
                     /locus_tag="VFV59_03695"
     CDS             1022..1774
                     /locus_tag="VFV59_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5324965.1"
                     /translation="MTIVVAHRGASDRAPENTMEAFRLGVEDGADAIELDVHLTADGQ
                     LAVIHDDTLDRTTDRTGAVASMTMDQVREADAGAQFAGPDGSSPYRGRGLTVPTLGEV
                     LAWLPEGIGLVVEIKAAAAADAVVAAVAQHPVRDGERLAVISFDERAIERVRELDPAI
                     RTGYLLVPTQPIEPALVWATEHGHIGVFPWEGDLGLDPMPLLAQAKAYGRQIGCYVVN
                     DPERMQHLAACGLWAFVTDVPRLARQALPRGS"
     gene            complement(1771..2178)
                     /locus_tag="VFV59_03700"
     CDS             complement(1771..2178)
                     /locus_tag="VFV59_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5324966.1"
                     /translation="MRAFIIELENRPGTLADLCEAIGAANINVSGLAGASRHAGGAVA
                     LITSNDAGMRALLEARHADYREAPVVSATLDDRPGTLGEAARRLGDSGVNVEAIIPTG
                     VRGLRVSVAFAVDDEPRGRQALADLAQRATSSI"
     gene            complement(2193..3749)
                     /locus_tag="VFV59_03705"
     CDS             complement(2193..3749)
                     /locus_tag="VFV59_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEU5324967.1"
                     /translation="MSTDLEAALAAGYGFDAPALTFGAAMEGDQALPGAQVRVPLQMM
                     NRHGLVAGATGTGKTKTLQVLAEQLSDAGVPVFISDIKGDISGLAAPAQLNEKLTGRA
                     QSIGFADYAPKAFPVEFFTLDREGKGVRLRASVSSFGPILLAKVLDLNETQQSVLALA
                     FKYADDKKLPLINLEDLRALLNHLGSDEGKSEMAEYGGVATATVGVIIRKIVELEQQE
                     ADAFFGSPEFDVADFLRVTPEGNGVVSILELADMQDRPALFSTFMMWLLASLYETLPE
                     VGDPEKPKLVFFFDEAHLLFRDANKTFMQQIELVARLIRSKGVGVFFVTHSPSDVPAE
                     VLGQLGNRVQHALRAFTPDDLEVVRATAETFPTTEYYDVEKELTALGTGEALVTALDL
                     KGRPLPTARVMMRPPMSLMAQIEPSAFDAVVSASPLATEYASDTDPESAKELLAARMA
                     RVEADAQSSMDDVEDPEGRGRMRSTRAPERGIDWGDVAQEGARFARSGAFNTILRSIL
                     GILGGGSRRR"
     gene            complement(3762..>4020)
                     /locus_tag="VFV59_03710"
     CDS             complement(3762..>4020)
                     /locus_tag="VFV59_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5324968.1"
                     /translation="TTPLLIQHAEKDLRCTITQAEELFAVLRSLRRTVRLMRVPDESH
                     ELTRSGTPFRRVDNLRLIAEWFDHFLVQGKRRLPRIDPNRG"
BASE COUNT          529 a         1437 c         1419 g          635 t
ORIGIN      
        1 ccctgtgcat cggcggcacg gtcgtcatcg gcatcgtcga catccggctc gggctggtgg
       61 ccttcgccct ggccaacttc gcctaccagg cggcgctcat ctactacgac gcgctgctgc
      121 ccgacgtcgc ccatccgctg actcgcggcc ggctctcggg ggtgggcgtc gccctgggct
      181 actgcgggac gctgctcgtc ggcgtcatcc tgctgctggg cgtgaccgtc gagaacgacc
      241 gatcgacgcc ggccaccttc gcgctcgtcg gcggcctctt cgcgctgttc gcggcgccgc
      301 tcttcctgct ggtgcgggag ccggcccgcg gcggtgccgc gttcagtgcc atcgacgcgg
      361 tccgctcgtg gcgacagctg gccgacacgc tcgaccacgc gcggcgggcg ccggggctgc
      421 tccggttcat cgtggcgcgc ttcttctact ccgacccggt gaacaccacc atcgccgtca
      481 tgagcgcctt cgcggtcaac gccatcggct tcaccgaggg ccaggcgctg caggtgctgc
      541 tggtcctgac cgtggtcgcc gtgctcgcct cgttcggctg gggcgtgctg gccgaccgct
      601 ggggaccgcg ccggaccctc ttcgcggtgc tggccgcctg ggccctcgcg ctcgccatcc
      661 tgaccgcgtt cctctcgacc atccccttcc tcgtggccgg cgcgctggcc ggtgccgggc
      721 tgggcggcgt gggcgtggtg gatcggctga tgctcctgcg cctgacgcca ccggagcgcg
      781 tgggcgagat gttcggcctc tacggcctgg tcgggaagtt cagcgccgtc gtcggaccgg
      841 tcgtgtacgg catcatcgtg gcgaacctgc tggactcgct ggaccgcggt gcctaccagg
      901 tcgcggtcgg ctcgctgctc gtcctgctgc tcgtcggcat cgccatcctg cgcggcgtcc
      961 cgtcgggtga cgaggagaac gagctggccg ccgccgaggt gctgttggag gagacggacc
     1021 gatgacgatc gtggtggcgc accgcggtgc ctccgaccgg gcaccggaga acacgatgga
     1081 ggccttccgc ctcggcgtgg aggacggcgc cgatgccatc gagctcgacg tccacctcac
     1141 cgccgacggg cagctcgccg tcatccacga cgacacgctc gacagaacca ccgatcgcac
     1201 cggggccgtc gcgtcgatga ccatggacca ggtgcgcgag gcagatgcgg gcgcacagtt
     1261 cgccggcccg gacggctcat ccccgtaccg gggccgcggc ctgaccgtgc cgacgctcgg
     1321 cgaggtgctc gcctggctgc cggagggcat cggactggtc gtcgagatca aggccgccgc
     1381 ggccgccgac gccgtggtgg cggccgtggc gcagcacccc gtgcgcgacg gggagcggct
     1441 ggcggtcatc agcttcgacg agcgcgccat cgagcgcgtc cgcgagctgg accctgccat
     1501 ccggaccggc tacctgctcg tgcccacgca gccgatcgag ccggcgctgg tgtgggcgac
     1561 ggagcatggc cacatcggcg tcttcccgtg ggagggtgac ctgggactgg atccgatgcc
     1621 actgctggcg caggcgaagg cctacggccg gcagatcggc tgctacgtcg tcaacgatcc
     1681 cgagcggatg cagcacctgg ccgcctgcgg cctgtgggcg ttcgtcaccg acgtgccccg
     1741 gctcgcacgc caggcgctgc cgcgcggctc ctagatcgag ctcgtcgccc gctgcgccag
     1801 gtcggccagc gcctgccggc ccctcggctc gtcgtcgacg gcgaaggcga cgctgacacg
     1861 caggccgcgg acaccggtcg ggatgatggc ctcgacgttc acaccgctgt cccccagccg
     1921 gcgcgccgcc tcaccgagcg tgcccggccg atcgtcgagc gtcgccgaga ccacgggcgc
     1981 ctcccggtag tcggcgtgcc gcgcctcgag cagtgcccgc atgccggcgt cgttgctggt
     2041 gatcagcgcg acggcgccgc ccgcgtgccg ggaggcaccg gccagaccgc tgacgttgat
     2101 gttcgccgcc ccgatggcct cgcacaggtc ggccagcgtc ccggggcggt tctccagctc
     2161 gatgatgaat gcgcgcatcg gtactcctcc ggtcagcggc gacgtgagcc gccacccagg
     2221 atgccgagga tggagcgcag gatggtgttg aaggctccgg agcgggcgaa ccgcgcccct
     2281 tcctgtgcca cgtcgcccca gtcgatcccc cgctccgggg cgcgcgtcga tcgcatacgg
     2341 ccccggccct cgggatcctc gacatcgtcc atggagctct gcgcatcggc ttcgaccctg
     2401 gccatgcgcg cggccagcag ctccttggcg gactcggggt ccgtatcgga ggcgtactcc
     2461 gtcgccagcg gcgatgcgct caccaccgcg tcgaaggccg acggctcgat ctgggccatc
     2521 agcgacatcg gcgggcgcat catcacccgc gccgtcggca gcggtcggcc cttgaggtcc
     2581 agcgccgtca ccagcgcctc gccggtgccc agggcagtca gctccttctc gacgtcgtag
     2641 tactcggtcg tcgggaacgt ctccgcggtg gcgcggacca cctcgaggtc gtccggcgtg
     2701 aaggcgcgca acgcgtgctg cacccggttg cccagctgtc ccagcacctc ggccgggacg
     2761 tcggagggcg agtgggtcac gaagaacacg ccgaccccct tggatcggat caggcgagcg
     2821 accagctcga tctgctgcat gaacgtcttg ttcgcatcgc ggaacagcag gtgcgcctcg
     2881 tcgaagaaga agaccagctt gggcttctcc gggtcgccga cctcgggcag cgtctcgtac
     2941 agggacgcga gcaaccacat catgaaggtg ctgaagaggg ccgggcggtc ctgcatgtcg
     3001 gccagctcca ggatgctgac gacgccgttc ccctcgggcg tcacgcgcag gaagtcggcg
     3061 acgtcgaact cgggggagcc gaagaacgca tcggcctcct gctgctcgag ctcgacgatc
     3121 ttgcggatga tgaccccgac ggtggcggtc gccacgccgc cgtactcggc catctcggac
     3181 ttaccctcgt ccgatcccag gtggttgagc agcgcccgca ggtcctcgag gttgatgagg
     3241 ggcagcttct tgtcgtcggc gtacttgaag gcgagggcca gcaccgactg ctgcgtctcg
     3301 ttcaggtcca gcaccttcgc cagcaggatc ggcccgaagc tcgagaccga tgcgcgcagg
     3361 cgcacgccct tgccctcgcg gtcgagggtg aagaactcca ccgggaacgc cttcggagcg
     3421 tagtcggcga agccgatgga ctgcgcccga ccggtgagct tctcgttgag ctgggccgga
     3481 gccgccagcc cgctgatgtc gcccttgatg tcgctgatga agaccgggac gccggcatcg
     3541 ctgagctgct cggccagcac ctgcagcgtc ttggtcttgc ccgtcccggt cgcgccggcc
     3601 accaggccgt ggcggttcat catctgcagc ggcacgcgga cctgggcgcc gggcagtgcc
     3661 tggtcgccct ccatggcggc cccgaaggtc agtgccggcg cgtcgaagcc atagccagcg
     3721 gcaagggccg cctcgaggtc ggtggacatg ccgaagtctc cttacccgcg gttcgggtcg
     3781 attcgtggca gccggcgctt gccctgcacc aggaagtggt cgaaccactc ggcgatgagc
     3841 cgcaggttgt ccacgcgccg gaacggcgtg ccggagcggg tgagctcgtg cgactcgtcc
     3901 ggcacgcgca tcaggcgcac ggtccggcgc agggagcgca ggaccgcgaa cagctcctcg
     3961 gcctgggtga tggtgcagcg caggtccttc tccgcgtgct ggatgagcag cggcgtggtg
//
LOCUS       DASTYP010000075         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1316347, whole genome shotgun sequence.
ACCESSION   DASTYP010000075 DASTYP010000000
VERSION     DASTYP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316347"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFV59_03715"
     CDS             complement(<1..371)
                     /locus_tag="VFV59_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU5324969.1"
                     /translation="MTAEILPPPRTSIGPVAWLRANLFSGPVSSLLTVVIGAALLWLG
                     LQIGTWAVTEARWSVVTNNLRLFLIGQFPADQAWRIWLNLLVISVLTGLSAGSLGSTT
                     RALAVTLSAVQVLLALLIVASP"
     gene            complement(368..1552)
                     /locus_tag="VFV59_03720"
     CDS             complement(368..1552)
                     /locus_tag="VFV59_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119978.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5324970.1"
                     /translation="MTAAPAPSSPHASVPLWRDLRVLRIAGQVLVLLIVAVVAAFAAR
                     NLMTAMNERGLGFGFNFLSRAAGFEIAESPIPYAPTDTYGEAFLVGLLNTLFVSIVGI
                     VLATALGIVVGVARLSPNWLVNRVAAAYVEVMRNTPLLVQLFIIYFAVFLQLPGVTDT
                     LRIGDAVFLNQRGVFVPAPQAQSGLLPWLGVLVAGIVIAVAARRWQRRREDAGHHGRH
                     AAAIGLCVFAVLVVAAWVALRPFELQAPVQERFNFVGGLRLSPEFTALLVGLVIYTAA
                     FIGEVVRGGIQAVRKGQVEAARALGLTEGEILRLVVFPQALRIIVPPVTSQYLNLAKN
                     SSLAIAIGYPDLFKVGQTMANQTGQPVPVMILVMGTYLVISLVTSALMNLYNRRVQVL
                     ER"
     gene            complement(1614..2756)
                     /locus_tag="VFV59_03725"
     CDS             complement(1614..2756)
                     /locus_tag="VFV59_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5324971.1"
                     /translation="MSRRVLSIVGLLVLALVVTACGDQAPASSDAGSGSPAESSAPTT
                     GAEETPVPTEGTGPSEGGRLALVQERGQLICGVNGSLPGFSFLDENGEMSGFDADFCR
                     ALAAAVLGDATAVEFRALSTDQRGPALQTGEVDVLIRNTTWTVSRDTSWGLFAPTTFY
                     DGQAIMVNASVGAETLEDLAGATVCVQSGTTTELNLADAFRQAGVEIETQVFADIDPT
                     YQAYEEGRCDAVTSDRSQLVARRTGFANPDDHVILDAVLSKEPLGPVAPIGDDAWFNV
                     VKWTVFATIEAEEQGITSDNVEETATSSEDPVVQRLLGVTPEGSEPFESGLGLDADWV
                     VNVISQVGNYAEIYDRNLGPDTPFDLDRGLNSLWTDGGLLYAPPYR"
     gene            complement(3060..3135)
                     /locus_tag="VFV59_03730"
     tRNA            complement(3060..3135)
                     /locus_tag="VFV59_03730"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3100..3102),aa:Ala,seq:cgc)
BASE COUNT          533 a         1068 c         1199 g          438 t
ORIGIN      
        1 ggcgaggcga cgatcaggag cgccagcagc acctggacgg ccgacagcgt caccgcgagc
       61 gcgcgggtcg tcgacccgag gctccccgct gagagcccgg tgaggaccga gatcacgagc
      121 aggttgagcc agatgcgcca cgcctggtcg gcggggaact gcccgatcag gaagagccgg
      181 aggttgttcg tcaccacgct ccagcgcgcc tccgtgaccg cccacgtgcc gatctggagc
      241 cccagccaca gcagcgcggc gccgatcacc acggtgagga ggctgctgac cggcccggag
      301 aagagattgg ctcgcagcca ggccaccggc ccgatgctcg tgcgcggcgg cggcaggatc
      361 tcggcggtca tcgctcgagc acctggacgc ggcggttgta caggttcatg agcgcggacg
      421 tgaccaggct gatcacgagg taggtgccca tgaccaggat catcaccggc accggctggc
      481 cggtctggtt ggccatggtc tggccgacct tgaacaggtc ggggtagccg atggcgatgg
      541 cgaggctcga gttcttggcc aggttcaggt actggctcgt gaccggcggg acgatgatcc
      601 gcagcgcctg ggggaagacg accagccgca ggatctcgcc ctcggtcagc ccgagcgccc
      661 gcgccgcctc cacctggccc ttgcgcaccg cctggatgcc accgcgcacc acctcgccga
      721 tgaaggcggc ggtgtagatg accaggccaa cgagcagggc ggtgaactca ggcgacagcc
      781 gcaggccgcc gacgaagttg aagcgctcct ggactggcgc ctggagctcg aacggccgca
      841 gcgcgaccca ggcggcgacg accaggacgg cgaacacgca caggccgatg gcggcggcgt
      901 gccggccgtg gtgccccgcg tcctcgcgcc ggcgctgcca tcggcgtgcg gcgaccgcga
      961 tgacgatgcc cgccacgagg acgccgagcc agggcagcag cccgctctgc gcctgcggcg
     1021 ccgggacgaa gacaccgcgc tggttgagga agaccgcgtc gccgatgcgg agcgtatcgg
     1081 tcacgccggg aagctggagg aagaccgcga agtagatgat gaacagctgg acgaggagcg
     1141 gcgtgttgcg catcacctcg acgtacgcgg ccgcgacgcg gttgaccagc cagttggggc
     1201 tgaggcgggc gacgcccacc acgatcccca gggccgtggc cagcacgatg cccacgatgc
     1261 tgacgaacag ggtgttgagc aggccgacga ggaaggcctc gccgtaggtg tcggtcggcg
     1321 cgtaggggat cggcgactcg gcgatctcga agccggccgc tcggctgagg aagttgaagc
     1381 cgaacccgag cccgcgctcg ttcatcgcgg tcatcaggtt ccgcgccgcg aaggccgcga
     1441 cgaccgcgac gatcagcagg accaggacct gtcccgcgat gcgaagcacg cgcaggtcgc
     1501 gccagagcgg cacggaggcg tgcggtgacg aaggggcggg cgcggcggtc atcaggattc
     1561 gggagtcagg ggagggacgg agccagcggc cccatccctc cccgaacgga gggtcagcgg
     1621 tacggcgggg cgtacagcag gccgccgtcg gtccacagcg agttcagccc gcggtcgagg
     1681 tcgaacgggg tgtccggacc gaggttgcgg tcgtagatct cggcgtagtt gccgacctgg
     1741 ctgatcacgt tgaccaccca gtcggcatcc aggccgaggc ccgactcgaa gggctcggag
     1801 ccctccggcg tcacacccag caggcgctgg acgaccggat cctcgctgga ggtggcggtc
     1861 tcctcgacgt tgtcggaggt gatgccctgc tcctcggcct cgatggtcgc gaagacggtc
     1921 cacttcacca cgttgaacca ggcgtcgtca ccgatggggg ccaccggccc gagcggctcc
     1981 ttcgagagca ccgcgtccag gatgacgtgg tcgtccgggt tggcgaaccc ggtgcggcgg
     2041 gcgaccagct gcgaacggtc gctggtgacc gcgtcgcatc ggccctcctc gtacgcctgg
     2101 taggtcgggt cgatgtccgc gaagacctgc gtctcgatct cgacgccggc ctggcggaag
     2161 gcatcggcga ggttgagctc ggtcgtcgtg cccgactgga cgcagaccgt cgcaccggcc
     2221 aggtcctcga gcgtctcggc gccgaccgag gcgttcacca tgatcgcctg gccgtcgtag
     2281 aaggtcgtcg gggcgaagag gccccacgag gtgtcgcggc tgaccgtcca ggtcgtgttg
     2341 cggatgagga cgtcgacctc gccggtctgg agcgcgggtc cacgctgatc ggtcgacagc
     2401 gcgcggaact cgacggcggt cgcgtcgccc aacaccgccg cggcgagcgc gcggcagaag
     2461 tcggcgtcga agccgctcat ctcgccgttc tcgtcgagga agctgaagcc gggcaggctg
     2521 ccgttgacgc cacagatgag ctggccgcgc tcctggacga gcgccaggcg gccgccctcg
     2581 gacggaccgg tcccctcggt cgggacgggc gtctcctcgg cgccggtggt gggtgccgac
     2641 gactcggcgg gggatcctga gccggcgtcg ctcgacgccg gggcctggtc accacacgcg
     2701 gtgacgacga gcgccagcac gagcagaccg acgatggaca ggacacgtct cgacatggtg
     2761 gaaccaccct cccttggtgt gtcagtcgat aggtcgcatg cggcccgtgg ggccgtcggc
     2821 ccatcctaaa gggcgctggc aagcgttcgg tcgggtccgc gacctgctcc tgtgaggagc
     2881 attgcgtccg gggagacgtc cgggccggac gctgtggaag cacacgctgg tcgtggtgct
     2941 cccgtgagga gcagaagctc gtctggatgg cggctcggcc gacgcaatgc tcctgcggag
     3001 agcaccgcac acgggcgcga ctgagcagca gggcggaccg cgacgaggga agaagggggt
     3061 ggtggagatg aggggactcg aacccctgac ccccgcgatg cgaacgcggt gctctcccag
     3121 ctgagctaca tccccgggcc gcggggagta tagcaaaggc caagcgggcc ttccccaact
     3181 agaacatctg ttctatcatc ggttgtccga tgaggaccga tgccgccgcc gtctcgcc
//
LOCUS       DASTYP010000076        15293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_260994, whole genome shotgun sequence.
ACCESSION   DASTYP010000076 DASTYP010000000
VERSION     DASTYP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15293
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260994"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1471
                     /locus_tag="VFV59_03735"
     CDS             128..1471
                     /locus_tag="VFV59_03735"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5324972.1"
                     /translation="MTLRPPATAPRPYRFPDFERRELPNGMVAWLVPLPDRELVSVQL
                     LTDAGAAAEEEPQGGIAALTAQLLVTGTASLDASAFAETTERLGISVVSESSWDSARA
                     GFTALGTHLDEGTRLLAEMVRQPRLDEGEFERLKAERLNDILQARADPGRLADESFLR
                     HVYAPDTPYARMSAGVPKTVSGLGVADARTFHASRYAPSVADLVVAGAIDVDGAMAVI
                     ERHFGSWEGSGPGHRAFEPQPGGGRRVVLVDRPGSVQSELRVGHLGIDRYDPRYFPAI
                     VMAALLGGVFGSRLNLRLREQLGYTYGARCSFDPRRSAGPFTALAAVQTEVTVDAVRE
                     LLGQLERIREAPPGADELREVRDFLVGVFPLRFETTGGIAAAIEPLAVYDLPSDWWQR
                     YRERLEAVDPEAVHRAARDLVRPDDALILVVGDAARIGDELRAADFGPLEVVSGP"
     gene            complement(1468..3039)
                     /locus_tag="VFV59_03740"
     CDS             complement(1468..3039)
                     /locus_tag="VFV59_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEU5324973.1"
                     /translation="MRFSGNDRRLVLGMIAGLAAAVAGIILAGSDQALPYTVIPVLGV
                     ILVGLTLGSGAAMGAYIVATLILVLLTLQPSHIDDLEASDVVRLTAFLLGTPLAVLLV
                     NRAEVDRRRADRALRASEEAGAALEAERQRLEAARREVDSALATAERERARLEEVAES
                     IPEPLIVYDEDGHGTYGNRAALRLFGRSFVERSLDAWGRVTQPRDVRGRVLAPEEWPQ
                     VRAQHEPVRGRLTLRIPMTGRDLMVDVEGTPIPGGGAVLLLRDIGKEEDERRRLSRFA
                     SFVAHELRNPLAVAKARIELAQRDPQRGERARAHDGRALESVDAAIGILERLELYSRA
                     DSGRVEASREPFELAPAVGSAIERLRARGSQREVRVAMAPGAWVTGDRQLSEQAITNL
                     LTNADRYATANTPIRVSVEAGAQVVLRVADDGPGIDDEIADRIFRERVTAGRGLGLGL
                     YLVRATMEAMGGSVVLEQRRPGSVFALRWPVGQSPTVDTVTDQADHADHADHADHADE
                     RDRVDDATRTAPAER"
     gene            complement(3084..4223)
                     /locus_tag="VFV59_03745"
     CDS             complement(3084..4223)
                     /locus_tag="VFV59_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU5324974.1"
                     /translation="MGQTDRIPTEERDYYEVLGAEPSASSAELRTAFREAVLRHHPDR
                     APTSDVATRRTSILNRAWAELRDPLRRLHYDRELEAGRAATMDWPLEQHEAHRVRPRR
                     VRRPDIGAPSPWHQPQWRSVGGFRIPAEVFLAGPAAQDRWIVEHHIRGEDWREHSERY
                     WLRYAARHYADHGRVDDWLGTIERIVALDAPFAELVDLHIREAYVAAEEELRGAMTLA
                     RLVDRYPDGTAQRRWAERELRVLLGEYRDRHVRRGPAARRAENAELLLNFLETLGVEP
                     SFTDYRAAIVAHRRAGHGGRAAELVERVMAAPVTDPGRWFSLVQLLTEAGQLDRASAL
                     LAEIARGEHPEALNPRRVVGPWRRIAVARERLARARRRSGGRATA"
     gene            4261..5250
                     /locus_tag="VFV59_03750"
     CDS             4261..5250
                     /locus_tag="VFV59_03750"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEU5324975.1"
                     /translation="MPATARSGQARTGHLLIIGGAEDKLRQRQILTRFVALAGGNDAR
                     VVVISTASSLGDEATELYLELFRQLGITDVRGLRPLVREDADDRRLAATLEDATGIFM
                     TGGNQLRLSSVIGGTALGRALVERHRHGAIIAGTSAGASAIASHMVAFGTSGATPKQR
                     MTQMSAGLGLLPGVLIDQHFEQRNRFGRLLALVAQSPALLGIGIDEDTAGLVTPKGVL
                     EVIGKGSVTILDPAQIQTDAYEVKRHRPIMVSGVVLHSLPSGYRFDIRRRKLLAPLRP
                     ISATDRELASLQAASAQTRRLIRRIAAEGADDTAPERARRRALHARRMEEASE"
     gene            5247..8084
                     /gene="cphA"
                     /locus_tag="VFV59_03755"
     CDS             5247..8084
                     /gene="cphA"
                     /locus_tag="VFV59_03755"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU5324976.1"
                     /translation="MSAVERAPRGGKGTAPRRQKATPTPPAGVEPKAELRILETRVYR
                     GPNYWSYDPAIKLIVDLGVLEHYPSNTLPGFVEALLEMLPGIAEHSCGTGKVGGFAGR
                     LREGTWVGHIAEHIALQLQREAGTEVGRGKTRGTGEPGRYHVVYSYAEETVGLAAGKL
                     AVRLVNHLVEAEPGFDFGAELEALILLAERAAFGPSTQAILDEAALRDIPSIRLNEQS
                     LVQLGHGVHQKRIRATMTSQTASLGVDIASDKKLTNRLLAATGVPVPRSEVVRDEDSA
                     AAAAIRIGYPVAVKPLDGNHGRGVMLNLVDEVSVRDAYGIARAESRNGGVVVETYLTG
                     NDYRCLVIGGVLRAVAQRVPAHVEGDGSRSLAELVEITNADPRRGIGHEKVLTRIKID
                     DEAIAYAREQGFELSDVPPRGQRIYLKRTGNMSTGGISIDRTEEIHPDNAELAELAAQ
                     VVGLDIAGIDLICPDVSLPVRETGGGIVEVNAAPGFRMHTNPTEGEAQYVAKPVIDLL
                     FPPGTPSRIPIVAVTGTNGKTTTARMIAHIMKGMGRKVGLTSTDGIYVDGRIVRKADA
                     SGPRSASMVLQNPTVDFAVFEVARGGILREGLGYQRNDVAVVLNVTGDHLGLGGIHSI
                     RQLAEVKRVIVEAVPRDGVAVLNADDPLVAAMSRHCSGSVIYFSMDPEHDRLKFQASR
                     GRRAVTLEQGRNGEKIVLRQGRKTMDLVWTHLIPATFEGRARMNVQNALAAAAAAWAA
                     GAHLHDIRQGLRTFTTSYFMAPGRLNMFELDGYRVVVDYAHNPPAVESLGEFVERLSE
                     PSAGGARAPVTGRRIAVVATAGDRREEDIRELGRVAARYFDEIIVREDENNRGRPRGS
                     TAELIVEGIESAGSERRVGSVTTILDELQATRRALDLATDGDVVVVCVDHANQVWKEL
                     QRRQHGAASETEGLRAVVGVSDSDEEIDLDL"
     gene            complement(8081..9712)
                     /locus_tag="VFV59_03760"
     CDS             complement(8081..9712)
                     /locus_tag="VFV59_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5324977.1"
                     /translation="MAPEPEAEPQPSDTEVDLVELRVLDGPNRFFTRPAVKLEFRAEE
                     PGRAAEAAASAALAVRRLHVALDLPEPKLTARHSTDGQRSAVAFPWRRRTISQAIAAS
                     AARLALGKSTERRELSGLRAVAHGPLAHLPRPRIPIVAVTGTNGKSTTTRLIAHILAA
                     AGRAVGMTNSDGIYVRGELVEAGDWTGFGGASRILAEPGLDVAVLETARGGILLRGIG
                     YAANDVSVVTNISADHLGLQGIDTLDEMAEVKSTVARITRRDGWSVLNADDPRVWQMR
                     RLTKANIYAFSLDPRSGTVEAALDAGGRAAVLDRGRLVLRGAGRRPRGLLRVEDVPVT
                     FGGLSRFNIANALAAAAAADALGLTTRQINAGLRSFGQEADANPGRLNLFERDGTFAL
                     VDFAHNEAGMAGLLEVARAVARRGRVRLAFGTAGDRTDEILFRMGGIAARADDLVIAE
                     KEHYLRGRDLGEMNELLRSGAREGGYAGEIEAHPSELQALRVLLDRARRGDVACVMAH
                     VERRELFAWLREQGFRAVTPEDLRRLVAGRAAPAG"
     gene            9735..10901
                     /locus_tag="VFV59_03765"
     CDS             9735..10901
                     /locus_tag="VFV59_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5324978.1"
                     /translation="MPDLDALRRTVDARLPRFREELESLVNIDCGSWTPEGVNQVADA
                     VADALERLGAEVERRPHHPAEGKRQLGDLVIGRLAGDGPRLLLIGHMDTVFDPGTAAA
                     RPYRSDGRRAWGPGVTDMKAGLLAGLHAIGALHEVGVHPAVTFVANPDEEVGSQFSTP
                     VIRGLASHHDAVLVLECARANGDIVSARKGIADYHLTYVGRAAHAGVEPEKGRSAVLE
                     AARQVVALHALNGRWPSVTVNAGVIHGGTRPNVVAERCELQVDLRAATTEAFEAAAEE
                     IARLASNPADLDVRVDLQQVAGHPPMEKSAASARLVDLAVRIAGELGFALRDAATGGA
                     SDANTTAALGIPTVDGLGPIGGDDHSVDEWLDLESLGPRTTLLAALMARAGEAL"
     gene            10945..11145
                     /locus_tag="VFV59_03770"
     CDS             10945..11145
                     /locus_tag="VFV59_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324979.1"
                     /translation="MPTRRVLISIDDRLLARIDDACRRRGMTRSAYLAQLADADLVGG
                     IGPGADPRVRTALARIETLLQR"
     gene            11142..11522
                     /locus_tag="VFV59_03775"
     CDS             11142..11522
                     /locus_tag="VFV59_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324980.1"
                     /translation="MKRVVLDPGGFLAWFAVDGRPLRAEYEAGALEVHVPRSFGLDVL
                     ERIARERSSGAEDLARLADDIERLGFREHDVSPVDAAPHLQRGRTASQAAYVALSVRL
                     DLPLITDDARLLGSVPGARRPADA"
     gene            11558..12019
                     /locus_tag="VFV59_03780"
     CDS             11558..12019
                     /locus_tag="VFV59_03780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5324981.1"
                     /translation="MTDAYRHRTSLQVRFGDTDAFGHVNNAVFFSYVELARIRYLLDV
                     LKPPTPFDRMPLILARVELDFRSPIFFGDEVRVETRVDRIGRSSFDMRHRMTAGEDDR
                     LVGDVHTVLVTYDYTASRPIPVPDDWRRAFAEHEERTFETDAPRPAGAAAS"
     gene            12035..13453
                     /gene="lpdA"
                     /locus_tag="VFV59_03785"
     CDS             12035..13453
                     /gene="lpdA"
                     /locus_tag="VFV59_03785"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU5324982.1"
                     /translation="MADQQRFDLLILGGGMSYVGAIRAAQLGLSVGIVERDRLGGTCL
                     NRGCIPSKALLETADLLHRVTEQGAEFGLSGHDGIGLDYGALGKRRDAVVEKHVNGVE
                     FLMKKNKITVLRGQGVLTGPTSVRVSGGESGDVDASATNLILATGSAPRSLPGLEPDG
                     QLIITSDEALARNDAPKRVAIVGAGAIGVEWASMYRDFGAEVTVIEYLDRVVPLEDPD
                     ISKELARTFRKRGITAHVGSTIDPESLRRGDGEISFTIATKDGSKRTDLTTDLILVAV
                     GRRPLTDGIGLEGIDGLKTERGYVIVDEFMRTGVPNLYAIGDIVPGFALAHVASHESV
                     VAVEHMTGHDPEPVRMELMPRVTFCRPQIASVGKTEEEAKAERRSVKTGQFPFRALGK
                     ATIVGEVDGFAKFVADAETGLLLGTHIIGPHAGDLIAEPTFAQLVEGTAGEIAMNVHA
                     HPTLPEVLPEAAMAVDGAPIHV"
     gene            13484..14545
                     /locus_tag="VFV59_03790"
     CDS             13484..14545
                     /locus_tag="VFV59_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5324983.1"
                     /translation="MASTDLQPRHAQLGLSDDDVLAMYRAMLLARAVDERMWLMQRAG
                     KIAFIISGQGHEGAQVATSWPMRRGQDWMAPYYRSIASTIAFGMSAEDIITAHLAKGD
                     DVSSGGRQMPGHYGGAPYNIVSLSSPVGTQVLHAVGIAMGAWVRGDDVVTMTQFGEGT
                     SNQGEIHEAMNFAGVHRLPVIFVCENNGYAISVPLERQVAGGSVAARAEGYGMPGVVV
                     DGGDVLACYAAAKEAHERARRGEGPTLIEARVVRLTSHSSDDDQRRYRDPAEVESLKE
                     HDPIPTFAATLREAGVLTDEVDERLRADVKAEVNDASKRAEARPEPTADDAHERVYAE
                     PIPGGGTATPLAPIEGGSH"
     gene            14545..>15293
                     /locus_tag="VFV59_03795"
     CDS             14545..>15293
                     /locus_tag="VFV59_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020061553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5324984.1"
                     /translation="MAELNILEAIRSAMDEEMERDERVMVMGEDVGKKGGVFGATEGL
                     WAKYGDKRVLDTPLTEGMIAGAAMGAAVYGLRPVAEMQFADFVYPAFNQIVSEISRIH
                     YRSNGTFTAPMVIRMPYGGGVHGALYHSQSNETYFTSTVGLKVVAPGTPADAKGLLKS
                     AIRDPDPVMFFEHKKTYRLFRAQVPDDDTVVPIGKAAVAREGRHLSIFCYGYMRTVCL
                     EAAETLAREDGVEAEVIDLRTLRPLDLETVLG"
BASE COUNT         2197 a         5569 c         5510 g         2017 t
ORIGIN      
        1 accgcatcaa ccacgaggtc gagcgctacg acgcggtcga cgccgatcgg gtgcgcgcgt
       61 ccatgcaggc cttcctggtg cccgacaacc gggtgctcct cacctacgtt cccgcggagg
      121 cgaaggcatg acgctccgac ccccggccac ggccccgcgg ccgtaccggt ttcccgactt
      181 cgagcggcgc gagctgccga acggcatggt ggcgtggctg gtgccgctcc ccgaccgcga
      241 gctggtcagc gtccagctcc tgaccgatgc cggcgccgcg gccgaggagg agccgcaggg
      301 cggcattgcc gcgttgacgg cgcagctgct ggtgaccggc accgcatccc tggatgcgtc
      361 ggccttcgcc gagacgaccg agcggctggg catcagcgtg gtcagcgagt cgtcctggga
      421 cagcgcgcgg gccggcttca ccgcgctggg gacccacctg gacgagggca cgcgcctgct
      481 ggccgagatg gtgcggcagc cgcggctcga cgagggggag ttcgaacgcc tcaaggccga
      541 gcgcctcaac gacatcctcc aggctcgggc cgatcccggc cgcctggcgg acgagagctt
      601 cctgcgccac gtctacgcgc ctgacacgcc gtacgcgcgc atgtcggcgg gcgtgcccaa
      661 gacggtgagc ggactgggcg tggcagacgc gcgcaccttc cacgcgtcgc gctacgcgcc
      721 aagcgtcgcg gacctggtcg tggccggcgc gatcgacgtg gacggcgcga tggcggtgat
      781 cgagcgccac ttcgggtcgt gggaggggtc gggtcccggg caccgcgcct tcgagcccca
      841 gcccggaggc gggcggcggg tggtgctggt tgaccgtccc ggatcggtgc agagcgagct
      901 gcgggtcggg cacctgggca tcgaccgcta cgacccgcgc tacttccccg ccatcgtcat
      961 ggcggcgctg ctcggcggcg tcttcggcag ccgcctgaac ctccggctgc gggagcagct
     1021 gggctacacg tacggcgccc gctgctcgtt cgacccgcgc cggtccgctg gcccgttcac
     1081 ggcgctggcc gccgtccaga ccgaggtgac cgtcgacgcc gtgcgcgagc tgctgggcca
     1141 gctcgaacgc atccgcgagg cgccgccggg agccgatgag ctccgcgagg tccgcgactt
     1201 cctggtcggc gtcttcccgc tccggttcga gacgaccggc gggatcgccg cggccatcga
     1261 gccgctggcg gtctacgacc tgccgtccga ctggtggcag cgctaccgcg agcggctgga
     1321 ggcggtggac ccggaggcgg tgcaccgggc cgcgcgggac ctggtccgac cggatgacgc
     1381 gctgatcctg gtcgtgggcg acgccgcgcg catcggcgac gagctgcgcg cggcagattt
     1441 cggcccgctc gaggtcgtca gcgggcccta gcgctccgca ggtgccgtcc gggtcgcgtc
     1501 gtccacgcgg tcccgctcgt ccgcgtggtc cgcgtggtcc gcgtggtccg cgtggtccgc
     1561 ctggtccgtg acggtgtcca cggtcggcga ctgcccgacg ggccagcgca gggcgaacac
     1621 cgaaccgggg cgccgctgct cgagcacgac cgatccgccc atcgcctcca tcgtggcgcg
     1681 gacgaggtac aggcccaggc cgagcccgcg gccggccgtg acccgctcgc ggaagatgcg
     1741 gtcagcaatc tcgtcgtcga tgcccggccc gtcgtcggca acgcgcagga ccacctgcgc
     1801 cccggcctcg accgagaccc ggatgggcgt gttggccgtg gcgtagcgat cggcgttggt
     1861 cagcaggttg gtgatggcct gctcgctgag ctgtcggtcg ccggtgaccc aggcgcccgg
     1921 cgccatggcg acgcgaacct cgcgttgcga cccacgcgcg cggagccgct cgatcgccga
     1981 tccgaccgcc ggggccagct cgaagggctc gcgggacgcc tcgacgcgtc cggaatcggc
     2041 gcgcgagtag agctcgaggc gctcgaggat gccgatcgcc gcgtccaccg actcgagggc
     2101 gcgcccgtcg tgagcccgcg ctcgctcgcc gcgctgcggg tcccgctggg cgagctcgat
     2161 gcgcgccttg gccaccgcca gcgggttgcg cagctcgtgc gccacgaagc tcgcgaagcg
     2221 agacaggcga cgccgctcgt cctcctcctt gccgatgtcc cggagcagca ggacggcgcc
     2281 accgccgggg atcggcgtcc cctcaacgtc gaccatcaga tcgcgcccgg tcatcgggat
     2341 gcgcaacgtg aggcgaccgc gaaccggctc gtgctgcgcc cgcacctgcg gccactcctc
     2401 gggggccagc acgcggccgc ggacatcccg aggctgggtg acccgccccc acgcatcgag
     2461 cgagcgctcg acgaagctgc gaccgaagag gcgcagcgcc gcccggttgc cgtaggttcc
     2521 atggccgtcc tcgtcataca cgatcaacgg ctccgggatc gactcagcga cctcctcgag
     2581 tcgagcgcgc tcgcgctccg ccgtggcgag ggccgagtcg acctcccgac gggcggcctc
     2641 cagccgctgc cgctcggcct ccagcgccgc gcccgcctcc tcgctggccc gaagcgcccg
     2701 atcggcccga cgccggtcga cctccgcccg gttgacgagc agcacggcga gcggggtccc
     2761 gagcaggaag gcggtgaggc gaacgacgtc agacgcctcg aggtcgtcga tgtgcgacgg
     2821 ctgcagcgtc agcaggacga ggatgagcgt ggcgacgatg tacgcgccca tcgccgcgcc
     2881 ggagccgagc gtcagcccga ccaggatgac cccgagcacc gggatgaccg tgtagggcaa
     2941 tgcctggtcg ctgcccgcca ggatgatccc cgccacggct gcagccagcc cggcgatcat
     3001 gccgagcacc agccgccggt cgttgccgct gaacctcacg gggcgcctgc tccgatgcgg
     3061 atgggccccc gagcctagca cgctcaggcc gtcgcccggc ctccggacct ccgtcgagcg
     3121 cgagccaacc gctcgcgtgc cacggcgatg cgccgccacg gcccgacgac ccgcctgggg
     3181 ttgagcgcct cggggtgctc gccgcgcgcg atctccgcca ggagcgccga tgcgcgatcg
     3241 agctggcccg cctcggtcag cagttgcacc aggctgaacc agcgcccggg gtcggtcacc
     3301 ggcgcggcca tcacacgctc caccagctcc gcggcgcgac cgccgtgacc cgctcggcgg
     3361 tgcgccacga tggcggcacg gtagtcggtg aaggacggct ccacgcccag cgtctcgagg
     3421 aagttgagca gcagctccgc gttctcggcg cgtcgcgcgg cgggtccgcg acgcacgtgc
     3481 cggtcgcggt actcgccgag caggacgcgc agctcgcgct ccgcccaccg ccgttgcgcc
     3541 gtgccgtccg ggtagcggtc caccaggcga gccagcgtca tcgcgccgcg cagctcctcc
     3601 tccgcggcga cgtacgcctc ccggatgtgg agatccacca gctcggcgaa gggcgcgtcg
     3661 agcgccacga tccgctcgat cgtccccagc cagtcgtcga cgcggccgtg gtcggcgtag
     3721 tgcctcgccg cgtagcgaag ccagtagcgc tcggagtgct cgcgccagtc ctcgccgcgg
     3781 atgtggtgct cgacgatcca gcggtcctgc gccgcgggac cggccaggaa cacctcggcg
     3841 ggaatgcgga acccaccgac cgagcgccac tgcggctggt gccagggact gggggcaccg
     3901 atgtcgggac ggcggacgcg gcgcggccga acgcggtgcg cctcgtgctg ctcgagcggc
     3961 cagtccatgg tcgccgctcg gccggcctcg agctcgcggt cgtagtgcag gcgacgcagc
     4021 gggtcgcgca gctcggccca ggcgcggttg aggatcgagg tgcgacgggt ggcgacgtcg
     4081 gaggtcggcg cgcggtcggg atggtgccgc aggacggcct cgcggaacgc cgtgcgcagc
     4141 tccgccgagg acgccgaggg ctccgccccg aggacctcgt agtagtcccg ctcctcggtg
     4201 gggatgcggt ccgtctgccc catccgatcg agtctagcgg cgcccgctat gctgcgcgcc
     4261 atgcctgcca ccgcccgctc cgggcaggcc cggaccggcc acctgctcat catcggcggc
     4321 gccgaggaca agctgcgtca gcgacagatc ctgacgcggt tcgtcgcgct ggccggcggg
     4381 aacgacgcgc gcgtcgtggt gatctcgacg gcgtcgtcgc tgggtgacga ggcgaccgag
     4441 ctgtacctgg agctcttccg acagctgggc atcaccgacg tgcgcggcct gcggccgctg
     4501 gtgcgcgagg acgcggacga ccggcgcctc gcggcgacgc tcgaggacgc cacgggcatc
     4561 ttcatgacgg gcggcaacca gctgcggctg tcgagcgtga tcggcggcac cgccctcggc
     4621 cgagcgttgg tcgagcgcca tcgccacggc gcgatcatcg ccggcaccag tgccggcgca
     4681 tcggccatcg ccagccacat ggtggccttc gggaccagcg gcgccacccc gaaacagcgc
     4741 atgacgcaga tgagcgcggg cctcgggctg ctcccgggcg tgctgatcga tcagcacttc
     4801 gagcagcgca accggttcgg acggctgctg gccctggtgg cgcagtcgcc ggcgctgctg
     4861 ggcatcggaa tcgacgagga caccgctggc ctggtgacgc cgaagggcgt gctcgaagtc
     4921 atcggcaagg gatcggtcac catcctggat cccgcgcaga tccagaccga tgcgtacgag
     4981 gtcaagcgtc accggccgat catggtctcc ggcgtcgtgc tccactccct gccatccggc
     5041 taccggttcg acatccgccg gcggaagctc ctcgccccgc tgcggccgat cagcgcgacc
     5101 gaccgcgagc tcgcgagcct gcaggcggcg agcgcgcaga cccggcggct catccggcgc
     5161 atcgccgcgg agggagccga tgacaccgcg cccgagcgag ctcgacgccg cgcgctccac
     5221 gcccggcgca tggaggaggc gagcgaatga gcgccgtcga gcgcgcgccg cgcggcggca
     5281 agggcacggc tccgcggcgc cagaaggcca cgccgacccc gcccgccggc gtcgagccga
     5341 aggccgagct gcgcatcctc gagacccgcg tctaccgcgg cccgaactac tggagctacg
     5401 acccggcaat caagctcatc gtggacctgg gcgtgctcga gcattacccg tcgaacacgc
     5461 tgccgggctt cgtcgaggcg ttgctggaga tgctgcccgg catcgcggag cacagctgcg
     5521 gcaccggcaa ggtgggcgga ttcgccggcc ggctgcgtga gggcacgtgg gtcgggcaca
     5581 tcgcggagca catcgccctc cagctgcagc gggaggccgg taccgaggtc ggacggggca
     5641 agacgcgtgg caccggggag ccgggccgct accacgtcgt ctactcgtac gccgaggaga
     5701 cggtggggct cgccgccggc aagctcgccg ttcggctggt caaccacctg gtggaggccg
     5761 aaccgggctt cgacttcggc gccgagctcg aggcgctcat cctgctggcg gagcgcgccg
     5821 ccttcggacc atcgacgcag gccatcctgg acgaggcggc gctgcgggac atcccctcca
     5881 tccggctcaa cgagcagtcg ctggtccagc tgggccacgg cgttcaccag aagcggatcc
     5941 gggcgaccat gaccagccag accgcgtcgc tcggcgtcga catcgcctcg gacaagaagc
     6001 tcaccaaccg gctgctggcg gccaccggcg tcccggtgcc gcgctccgag gtcgtgcgcg
     6061 acgaggactc ggctgccgcg gcggccatcc ggatcggcta cccggtggcg gtgaagcccc
     6121 tggacggcaa ccacggccgc ggcgtgatgc tcaacctggt cgacgaggtc tcggtccgtg
     6181 acgcgtacgg gatcgcgcgg gccgagagcc ggaacggcgg cgtggtggtc gagacgtacc
     6241 tcaccggcaa cgactaccgc tgcctggtga tcggcggcgt gctgcgcgct gtggcccagc
     6301 gggtcccggc ccacgtcgag ggcgacgggt cccgctcgct cgcggagctg gtcgagatca
     6361 ccaatgcgga cccgcgccgc ggcatcggcc acgagaaggt gctgacccgc atcaagatcg
     6421 acgacgaggc gattgcgtac gcgcgcgagc agggcttcga gctcagcgac gtgccgccgc
     6481 ggggccagcg gatctacctg aagcgcaccg gcaacatgag caccggcggc atcagcatcg
     6541 accggaccga ggagatccac cccgacaacg cggagctggc cgagctggcc gcccaggtgg
     6601 tggggctgga catcgccggc atcgacctga tctgccccga cgtcagcctg ccggtgcgcg
     6661 agaccggcgg cgggatcgtg gaggtcaacg cggcgcccgg gttccggatg cacaccaacc
     6721 cgaccgaggg cgaggcgcag tacgtggcca agccggtgat cgacctgctc ttcccgccgg
     6781 gcacgccctc ccgcatcccc atcgtggcgg tcaccggcac caacgggaag acgaccacgg
     6841 cccggatgat cgcgcacatc atgaagggca tgggccgcaa ggtcggcctg acgtccaccg
     6901 atgggatcta cgtcgacggc cgcatcgtgc ggaaggccga tgcctccggt ccgcgctccg
     6961 cctcgatggt cctgcagaac ccgaccgtcg acttcgccgt cttcgaggtc gcgcgcggcg
     7021 gcatcctgcg ggagggcctc ggctaccagc gcaacgacgt ggcggtcgtg ctcaacgtca
     7081 ccggcgacca tctgggcctg ggcggcatcc actcgatccg gcagctggcc gaggtgaagc
     7141 gcgtgatcgt cgaggcggtc ccgcgcgacg gcgtggcggt gctcaacgcg gacgacccgc
     7201 tggtggcggc catgtcgcgc cactgctcgg ggtcggtgat ctacttcagc atggaccctg
     7261 agcacgaccg actgaagttc caggcctctc gcggccgccg tgccgtgacg ctggagcagg
     7321 gccggaacgg cgagaagatc gtgctgcgac agggacgcaa gacgatggac ctggtgtgga
     7381 cgcacctgat cccggcgacc ttcgaggggc gcgcgcggat gaacgtccag aacgcgctgg
     7441 ccgcggccgc cgcggcgtgg gcggcggggg cgcatctgca cgacatccga caggggctgc
     7501 ggaccttcac cacctcctac ttcatggctc cgggtcgcct caacatgttc gagctggacg
     7561 gctaccgggt cgtcgtcgac tacgcgcaca acccgccggc cgtggagtcc ctcggcgagt
     7621 tcgtggagcg cctcagcgag ccctcggcgg gcggcgctcg tgcgccggtg acgggacgca
     7681 ggatcgcggt ggtcgccacc gccggcgacc ggcgcgagga ggacatccgc gagctcggtc
     7741 gcgtcgctgc acgctacttc gacgagatca tcgtccgcga ggacgagaac aaccgcggtc
     7801 gcccgcgcgg cagcacggcg gagctcatcg tggagggcat cgagagcgct gggtccgagc
     7861 ggcgcgtcgg gtcggtcacc accatcctgg acgagctgca ggcgacgcgc cgcgcgctcg
     7921 acctggcgac cgacggggac gtcgtggtgg tctgcgtcga ccacgccaac caggtctgga
     7981 aggagctgca gcggcgccag cacggcgcgg catccgagac cgagggcctg cgcgccgtcg
     8041 tcggcgtctc cgactccgac gaggagatcg acctggacct ctagccggcc ggcgccgccc
     8101 gtccggccac gagccggcgc aggtcctccg gcgtcacggc acggaagccc tgctcgcgga
     8161 gccatgcgaa cagctcgcgc cgctcgacat gcgccatgac gcacgccacg tcccctcgcc
     8221 tcgcccggtc cagcaggacg cgaagggcct gcagctcgga gggatgcgcc tcgatctccc
     8281 ccgcgtaccc accctcgcgc gcaccggagc gcaggagctc gttcatctcc cccaggtcgc
     8341 ggccgcgcag gtagtgctcc ttctcggcga tgaccaggtc gtcggcccgg gcggcgatgc
     8401 cgcccatgcg gaacaggatc tcatcggtgc ggtcgcccgc cgtgccgaac gccagccgca
     8461 cccggccgcg gcgagcgacg gcgcgagcga cttccagcag cccggccatg ccggcctcgt
     8521 tgtgggcgaa gtccaccagc gcgaaggtgc cgtcgcgctc gaagaggttg aggcgccccg
     8581 gattggcatc ggcctcctgg ccgaagctgc gcagcccggc gttgatctgc ctggtggtga
     8641 ggccgagggc gtccgcggcg gctgcggcgg ccagcgcgtt ggcgatgttg aagcgcgaca
     8701 ggccgccgaa cgtcacgggg acgtcctcca cccgcagcag gccccgggga cgacggccag
     8761 cgccgcgcag cacgaggcgt ccccggtcga gcaccgccgc ccgaccgccc gcgtccagtg
     8821 cggcctccac cgtgccggag cgcgggtcga ggctgaaggc atagatgttc gccttcgtca
     8881 gccggcgcat ctgccacacg cgcgggtcgt cggcgttcag cacgctccag ccgtcgcgcc
     8941 gagtgatccg cgccacggtc gacttgactt cggccatctc gtcgagcgtg tcgatgccct
     9001 gcagccccag gtggtcggcc gagatgttgg tcaccacgct gacgtcgttg gcggcgtagc
     9061 cgatgccgcg cagcaggatc ccgccccgcg cggtctcgag gaccgccacg tcgaggcccg
     9121 gctccgcgag gatccgggat gcgccgccga acccggtcca gtcccccgcc tcgaccagct
     9181 caccgcgcac gtagatgccg tcggagttcg tcatccccac cgcgcggccg gcggctgcga
     9241 ggatgtgcgc gatcagccgg gtggtcgtgc tcttgccgtt cgtcccggtc accgccacga
     9301 tggggatccg gggccgcgga aggtgggcga gcggcccgtg ggccactgcc cgaaggccgg
     9361 agagctcccg gcgttccgtc gacttcccga gcgcgaggcg cgccgccgac gcggcgatgg
     9421 cctgcgagat cgtccgccgc cgccagggga aggcgaccgc gctccgctgc ccgtcggtgg
     9481 agtgccgcgc ggtgagcttc ggctccggca gatcaagcgc gacgtgaagc cggcggaccg
     9541 ccagggccgc gctggctgcg gcttcggccg cccgaccagg ctcctcggcc cggaactcga
     9601 gcttcaccgc cggccgtgtg aagaatcggt tcggcccgtc gaggacgcgc agctcgacca
     9661 ggtccacctc ggtgtcgctc ggctggggct cggcctcggg ctccggcgcc atggcggtga
     9721 gtatattgac gtccatgccc gatctcgacg cgctgcggcg caccgtcgat gcgcgcctgc
     9781 cgcggttccg cgaggagctc gagtccctcg tcaacatcga ctgtggctcc tggacccccg
     9841 agggcgtcaa ccaggtggcc gatgcggtcg ccgacgcgct cgagcgcctg ggtgccgagg
     9901 tcgagcgccg gccgcaccat ccggccgagg ggaagcgcca gctgggcgac ctggtcatcg
     9961 gacgcctggc cggtgacggt ccgcggctgc tgctgatcgg ccacatggac acggtcttcg
    10021 accccgggac ggccgcggcg cggccgtatc ggtccgacgg tcgtcgcgcc tgggggccgg
    10081 gcgtgaccga catgaaggcg gggctcctgg cgggactgca cgccatcggc gccctgcacg
    10141 aggtcggcgt ccatccggcc gtcaccttcg tggccaaccc ggacgaggag gtcggctctc
    10201 agttcagcac gcccgtcatc cgcgggctgg catcgcacca cgacgccgtg ctcgtgctgg
    10261 agtgcgcgcg ggccaacggc gacatcgtca gcgctcggaa gggcatcgcc gactaccacc
    10321 tcacctacgt cggtcgcgcg gcgcacgccg gcgtcgagcc ggagaaggga cgctcggccg
    10381 tgctcgaggc ggcacgccag gtcgtggcgc tgcacgcgct gaacggccgc tggcccagcg
    10441 tcacggtcaa cgccggcgtc atccacggcg gaacgcggcc gaacgtggtg gccgagcgct
    10501 gcgagctcca ggtggacctg cgcgccgcca cgaccgaggc gttcgaggcc gcagccgagg
    10561 agatcgcgcg gttggcgtcc aatccggccg acctcgacgt gcgcgtcgac ctacagcagg
    10621 tcgccggcca tccgccgatg gagaagagcg ccgcgtcggc tcggctggtg gatctcgcgg
    10681 tgagaattgc gggcgagctg ggcttcgcgc tgcgcgacgc ggccaccggt ggcgcgtcgg
    10741 acgccaacac cacggcggcg ctgggcatcc ccaccgtgga cgggctgggt cccatcggtg
    10801 gcgacgacca ctccgtggac gagtggctgg acctcgagag cctcgggccc cggacgaccc
    10861 tgctggcggc gctcatggcc cgggccggcg aggcgctgta ggccccgtcg cgggcgtcgc
    10921 gtatatccgg atatactccg ccgcatgcca acgagacgcg tcctcatctc gatcgacgat
    10981 cgcctcctcg cccggatcga cgacgcctgc cgccggcgcg gcatgacccg cagcgcctac
    11041 ctcgcccagc tggcggacgc cgacctcgtc gggggcatcg gccccggcgc ggatccccgc
    11101 gtgcggacgg ccctcgcgcg catcgaaacg ctcctgcagc gatgaagcgc gtcgtcctgg
    11161 accccggcgg cttcctggcc tggttcgccg tggacggacg cccgctccgc gccgagtacg
    11221 aggcgggcgc cctcgaggtt cacgtgcctc gcagcttcgg gctggacgtg ctggagcgga
    11281 tcgcccgcga gcgttcgagc ggcgcggagg acctcgctcg cctggcggac gacatcgagc
    11341 gcctcgggtt ccgcgagcac gacgtctcgc ctgtcgacgc cgctccgcac ctccagcgcg
    11401 gtcgcacggc cagccaggcg gcgtacgtgg cgctgagcgt ccgcctcgac ctgccgctga
    11461 tcacggacga cgcccgcctc ctcgggtcgg tcccgggggc acggcgaccg gccgacgcct
    11521 gacccgtgcc cggccgggcg ggctagaatg gccgcccgtg accgatgcct accgtcaccg
    11581 aacgtcgctc caggtccgct tcggggacac ggacgcgttc gggcacgtca acaacgcggt
    11641 cttcttcagc tacgtcgagc tggcgcgcat ccgctacctg ctcgacgtcc tgaagccgcc
    11701 gacgccgttc gaccggatgc cgctgatcct ggcccgcgtg gagctcgact tccgctcgcc
    11761 gatcttcttc ggcgatgagg ttcgggtcga gacgcgcgtc gaccgcatcg ggcgatccag
    11821 cttcgacatg cgccaccgca tgaccgccgg cgaggacgat cggttggtcg gcgacgtgca
    11881 caccgtgctg gtgacgtacg actacaccgc ctcgcggccg atcccggtcc cggacgactg
    11941 gcgccgggcc ttcgccgagc acgaggagcg aaccttcgag accgatgcgc cgcgccccgc
    12001 cggggctgcg gcgagctgaa gcgacaggag accgatggcc gaccagcaac gattcgacct
    12061 gctcatcctc ggtgggggca tgagctacgt gggcgcgatc cgcgccgcgc agctgggcct
    12121 ctcggtcggc atcgtcgagc gtgaccgcct gggcgggacg tgcctcaatc gcggctgcat
    12181 cccgtccaag gccctgctcg agacggccga cctgctgcac cgcgtgaccg agcagggcgc
    12241 ggagttcggc ctctccggcc acgacggcat cggcctggac tacggggcgc tggggaagcg
    12301 ccgcgacgcc gtggtggaga agcacgtcaa cggcgtcgag ttcctgatga agaagaacaa
    12361 gatcaccgtc ctgcgcggcc agggcgtgct gaccggcccg accagcgtcc gcgtctcggg
    12421 cggcgagtcc ggcgacgtcg acgccagcgc cacgaacctg atcctggcca ccggctccgc
    12481 cccgcggtcc ctccccggcc tcgagcccga cggccagctg atcatcacct ccgacgaggc
    12541 cctggcccgc aacgacgccc cgaagcgggt ggcgatcgtg ggggccggcg ccatcggcgt
    12601 ggagtgggcc agcatgtacc gcgacttcgg cgccgaggtg accgtgatcg agtacctcga
    12661 ccgcgtggtg ccgctcgagg atccggacat cagcaaggag ctggctcgca ccttccgcaa
    12721 gcggggcatc accgcccacg tcggctcgac catcgacccc gagtcgctgc gcaggggcga
    12781 cggcgagatc agcttcacca tcgccaccaa ggacggctcg aagcgcaccg acctgacgac
    12841 ggacctgatc ctggtcgcgg tcgggcggcg gccgctgacc gatggcatcg gcctcgaggg
    12901 gatcgacggg ctgaagacgg agcgcggcta cgtcatcgtc gacgaattca tgcgcaccgg
    12961 cgtgccgaac ctgtacgcca tcggcgacat cgtgccgggc ttcgccctgg cgcacgtcgc
    13021 ctcgcacgaa tcggtggtcg ccgtcgagca catgaccggc cacgacccgg agccggtgcg
    13081 gatggagctg atgccgcgcg tcaccttctg ccgcccgcag atcgcctcgg tcgggaagac
    13141 cgaggaggag gccaaggcgg agcgccggag cgtgaagacc ggccagttcc ccttccgggc
    13201 gctgggcaag gccaccatcg tcggcgaggt agacgggttc gcgaagttcg tcgccgatgc
    13261 ggaaaccggc ctgctgctgg gcacccacat catcggcccg cacgcgggcg acctcattgc
    13321 cgagccgacc ttcgcccagc tggtggaggg cacggccggc gagatcgcca tgaacgtcca
    13381 cgcccacccg accctgccgg aggtgctgcc cgaggccgcc atggcggtcg acggcgcacc
    13441 gattcacgtc taggacgccg aaggagagga ggaccaccag ccgatggcaa gcacggacct
    13501 gcaaccccgg cacgcgcagc tgggcctcag cgatgacgac gtcctggcga tgtaccgcgc
    13561 catgctgctg gcccgcgccg tcgacgagcg gatgtggctc atgcagcggg cgggcaagat
    13621 cgccttcatc atctcggggc agggccacga gggcgcgcag gtggccactt cctggccgat
    13681 gcgccggggg caggactgga tggcgcccta ctaccgctcc atcgccagca ccatcgcgtt
    13741 cgggatgagc gccgaggaca tcatcaccgc ccatctggcc aagggcgacg acgtcagctc
    13801 gggcggccgc cagatgcccg ggcactacgg cggggcgccg tacaacatcg tcagcctcag
    13861 ctcgccggtg ggaacgcagg tcctccacgc cgtcggcatt gccatgggcg cctgggtccg
    13921 cggcgacgac gtggtgacca tgacccagtt cggcgagggc acctcgaacc agggcgagat
    13981 ccacgaggcc atgaacttcg ccggcgtgca ccggctgccg gtcatcttcg tgtgcgagaa
    14041 caacggctac gccatcagcg tccccctcga gcgccaggtg gccggcggat cggtcgcggc
    14101 ccgcgcggag ggctacggca tgccgggcgt ggtggtcgac ggcggcgacg tgctggcctg
    14161 ctacgccgcc gcgaaggagg cgcacgagcg cgcgcgccgc ggggagggac cgacgctcat
    14221 cgaggcccgc gtggttcgcc tcacctccca ctcatccgac gacgaccagc gccggtaccg
    14281 cgatccggcc gaggtcgaat cgctcaagga gcacgacccg atccccacgt tcgcggccac
    14341 cctgcgcgag gcgggcgtcc tgaccgatga ggtcgatgag cgactgcggg ccgacgtcaa
    14401 ggccgaggtg aacgacgcgt cgaagcgggc cgaagctcgc cccgagccga cggccgacga
    14461 cgcccacgag cgcgtctacg cggagccgat ccccgggggc ggcaccgcga ccccgctcgc
    14521 cccgatcgag ggaggcagtc actgatggcc gagctcaaca tcctggaagc catccgctcc
    14581 gccatggacg aggagatgga gcgtgacgag cgcgtcatgg tgatgggcga ggacgtcggc
    14641 aagaagggcg gcgtcttcgg cgcgaccgag ggtctgtggg ccaagtacgg cgacaagcgc
    14701 gtgctcgaca cgccgctgac cgagggaatg atcgctgggg ccgcgatggg tgctgcggtg
    14761 tacggcctgc ggcccgtcgc cgagatgcag ttcgccgact tcgtctaccc cgccttcaac
    14821 cagatcgtca gcgagatcag ccgcatccac taccgctcca acggcacctt caccgcgccc
    14881 atggtgatcc gcatgccgta cggcggcggc gttcacggcg cgctgtacca cagccagtcg
    14941 aacgagacgt acttcacgtc gaccgtcggg ctcaaggtcg tcgctcccgg cacgccggcc
    15001 gacgccaagg ggctgctgaa gtccgcgatc cgcgacccgg acccggtgat gttcttcgag
    15061 cacaagaaga cgtaccgcct cttcagggcc caggtccccg acgacgacac cgtcgtgccg
    15121 atcgggaagg ccgcggtggc gcgggaggga cggcacctct cgatcttctg ctacggctac
    15181 atgcgcaccg tctgcctgga ggcggccgag acgctggccc gcgaggatgg cgtcgaggcc
    15241 gaggtcatcg acctgcgcac gctgcgcccg ctggacctgg agacggtgct cgg
//
LOCUS       DASTYP010000077         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_734731, whole genome shotgun sequence.
ACCESSION   DASTYP010000077 DASTYP010000000
VERSION     DASTYP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734731"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1161)
                     /locus_tag="VFV59_03800"
     CDS             complement(226..1161)
                     /locus_tag="VFV59_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5324985.1"
                     /translation="MQRTPLIVLGIAVLAVVAIVAVLLMQPSRPGTGAGQSVQASASA
                     AQPSASASLDAELMNRRWTVLYVGTDHNAARETAGEPVNTDALLLVSLSADQSRLTLV
                     SLPRDTVDLPLADGSTYEHKINALYREQGMDALVGAMEELYGVPIDAHVLLDMDDFAS
                     LVSAVGSITVSPPEPLVDPKVDLDLAAGEQEIDGPTTLSYVRTRVDQDYGRMARQQEV
                     VMALISRLVDPETDVDLRAVLDGLDSLETDLPMDQLTTLVELARRAADASVERLVIGP
                     PLITFEGDRGDGRGYVLEADAGAIRDEVQGLIGEE"
     gene            complement(1473..2456)
                     /locus_tag="VFV59_03805"
     CDS             complement(1473..2456)
                     /locus_tag="VFV59_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEU5324986.1"
                     /translation="MRASRLVSLLLLLQTRGRMTAGQLASELEVSTRTIHRDVEALSE
                     SGVPIYAERGPHGGVQLVDGYRTRLTGMTAEEAEALFLSGVPGPAAELGLGTVMAAAQ
                     LKVLAALPGELRARASRLVERFHLDAAGWFQRGEAVDQLPAIAQAVWEGRRLRIGYDR
                     GDRVVERVVDPLGVVLKGGVWYLVGRVDDQVRTYRVSRAVSAKVIDEAFERPVGFDLA
                     AYWSESIAAYEREAERADVLVRIRPERAGELAEILGGEALASAHRLPEPDPDGWTRFR
                     LRIRWPREAPGRLLQLGGDLEVLGPPEVRERVAALARAALDRHNTRGQATE"
     gene            2570..>2925
                     /locus_tag="VFV59_03810"
     CDS             2570..>2925
                     /locus_tag="VFV59_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEU5324987.1"
                     /translation="MTDAPVTLADYEPLARELIEPGAWDYYVGGAGDERSLAENRAAW
                     DRIRLLPRVLVDVARRDLSTAAFGVPLAHPIVVAPMAAHDLAHPEAERASARGAADAG
                     ALYTLSTISSLPLEDVA"
BASE COUNT          415 a         1064 c         1058 g          388 t
ORIGIN      
        1 cgagggcgag gccgaggcgg ctgccgaggc cggtggtgcc gagggcgctc cggagcgcga
       61 ggagagcggc gaggaggccg gctccgagtc gtaactgtgg cgcagcgtcg ccgcttggtc
      121 cacgcgcacc gcaacggcac ggtggcccct gcgccgtacg tcctcaccgc ggacggtggt
      181 gtggagcgcc ggccacgcgc ggaacctccc gtcggctgcg cgtgatcact cctcgccgat
      241 gagcccctgc acctcgtccc ggatggcgcc ggcgtcggcc tcgagcacgt agccgcgacc
      301 gtcgccacgg tcgccctcga aggtgatgag cggcggcccg atgaccagcc gctcgaccga
      361 tgcatcggcg gcgcgacgcg ccagctcgac cagcgtcgtc agctggtcca tcgggaggtc
      421 ggtctcgagc gagtcgaggc cgtccaggac ggcgcgcagg tcgacatcgg tctcggggtc
      481 gaccagccgt gagatcagcg ccatgacgac ctcctgctgc cgcgccatgc gcccgtagtc
      541 ctggtcgacg cgggtccgca cgtacgacaa cgtcgtcgga ccgtcgatct cctgctcccc
      601 cgccgccagg tcgagatcga ccttggggtc taccagcggc tcgggcggcg agaccgtgat
      661 gctgccgacc gccgagacca gcgaagcgaa gtcgtccatg tcgagcagga cgtgcgcgtc
      721 gatcgggacg ccgtacagct cctccatggc accgaccagg gcgtccatcc cctgctcgcg
      781 gtagagcgcg ttgatcttgt gctcgtaggt cgagccatcg gccagcggca ggtcgaccgt
      841 gtcgcgcggc agcgagacga gcgtcaggcg tgattgatcc gccgagaggc tgaccagcag
      901 cagtgcatcg gtgttgaccg gctcgccggc agtctcccgg gccgcgttgt gatcggtgcc
      961 gacgtacagg acggtccatc ggcggttcat cagctcggca tccagcgaag ccgatgcgga
     1021 cggctgcgcc gccgaggcgg acgcctggac cgattggccg gcgcccgtcc cgggccggga
     1081 cggctgcatg aggaggacgg ccacgatggc cacgacggcg agcacggcga tgccgaggac
     1141 gatcaggggg gttcgctgca tcgggccatt ctgccgagcg cggccacggc tcgcagagat
     1201 cgccgcgaac ggctcccaat cctgacagga gttggcaggt atggggggta gcgtatcggc
     1261 gtagccacgg tgaggactca gctgatgttc gtagacgaga tggcacgact ccgggcgcag
     1321 gacctggagc gggagatgcg gcggagcgct cgaatccgag cgctgctccg ggagcggccc
     1381 cgtccgacgc gcctgctgcg cgggctgccg cgcctccggc cgcacccggc cgcgcccggg
     1441 ccctgagggc tggaagccgg ccggacgatc ggctactcgg tggcctggcc gcgggtgttg
     1501 tgccggtcga gggcggcgcg ggccagtgcg gcgacgcgct cacgaacctc gggcggcccg
     1561 agcacctcga ggtcgccgcc gagctgcagc aggcgaccgg gcgcctcgcg cggccagcgg
     1621 atgcgcagcc gaaaccgggt ccagccgtcc ggatccggct cggggagccg gtgcgcggac
     1681 gccagcgcct cgcccccgag gatctccgcc aactcgcccg cgcgctccgg tcggatccgg
     1741 acgaggacgt ccgcgcgctc ggcttcccgc tcgtacgcgg cgatcgattc gctccagtac
     1801 gccgccagat cgaagccgac cggacgttcg aacgcttcgt cgatgacctt ggcggagacg
     1861 gcgcgcgaca cccggtaggt cctgacctgg tcgtcaaccc gtccgacgag gtaccacaca
     1921 ccgcccttga gcacgacgcc caggggatcc accacgcgct ccaccacgcg gtcgccgcgg
     1981 tcgtacccga ttcggaggcg ccgcccctcc cacacggcct gcgcgatcgc cggcagctgg
     2041 tcgaccgcct cgcctcgctg gaaccagccg gccgcgtcga gatggaagcg ctcgaccagc
     2101 ctggaggcgc gggcgcggag ctcgccgggg agggcggcga gcaccttcag ctgggccgcc
     2161 gccatgaccg tgccgagccc cagctcggcg gccgggccag gtacgccgga caggaagagg
     2221 gcttccgcct cctcggccgt catcccggtg aggcgcgtgc ggtagccgtc gaccagctgc
     2281 acgccaccgt gcggaccgcg ctccgcgtag atcggaacgc ccgactccga cagcgcctcc
     2341 acgtcgcggt ggatggtgcg cgtcgacacc tccagctcgc tcgccagctg cccggcggtc
     2401 atccggccgc gggtctgcag cagcaggagc agcgagacga ggcgcgaggc acgcatgcgt
     2461 cgatggtacg gccgaatgtc gccacacaat ggcaactatt cgccggtcgc ctcctggcac
     2521 gcagccgatc caccgctcga ccgccacggg gggtggcatc atcggctgta tgaccgatgc
     2581 gcccgtcacc ctcgccgatt acgagccact ggcccgcgag ctcatcgagc ccggagcctg
     2641 ggactactac gtcggggggg ccggcgacga gcgctcgctg gccgagaacc gggcggcgtg
     2701 ggaccggatc cggctgctgc cgcgcgtcct ggtcgacgtc gcacgccgtg acctctcgac
     2761 ggccgccttc ggcgtcccgc tggcccatcc catcgtggtc gcccccatgg cggcgcacga
     2821 ccttgcccat cccgaggcgg agcgcgcgtc cgcgcgcggc gcggccgatg ccggcgcgct
     2881 ctacacgctc tcgaccatct cgtcgctgcc gttggaggac gtcgc
//
LOCUS       DASTYP010000078         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_661373, whole genome shotgun sequence.
ACCESSION   DASTYP010000078 DASTYP010000000
VERSION     DASTYP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661373"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VFV59_03815"
     CDS             complement(<1..989)
                     /locus_tag="VFV59_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU5324988.1"
                     /translation="MTRFAKLAIAAAVATFALIAAGGLVRATDSGLGCPDWPLCFGDW
                     IPPADLHAWIEHTHRLIAAVFVGPLVGAVGLITVFTERRRDRPLLWAAVVAGVLVIVQ
                     SVIGGAVVLQGLAAELVTIHLGMALTVFAAVILIAERAVHGPMPAAHARPGLTRLLAL
                     TALVIFAQMLLGSWVTGHHAGLAYADFPLMNGQVIPRIFGSEQAVQFAHRAMSVLVAV
                     MVLWSAVRVHRSTLSPLAHRLAVTMVVLIGIQIALGGLNVVSRLSAFFVVPHLAVGAA
                     LWGASVWLTLVSRRLARSELRHAVPDSEHEPGAGDTVRAYISLTKPRIIELLLV"
     gene            complement(1081..1923)
                     /locus_tag="VFV59_03820"
     CDS             complement(1081..1923)
                     /locus_tag="VFV59_03820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEU5324989.1"
                     /translation="MTDAPRADAHAALAEAEGLFGPGSVSWRIDRELVVLAGGSCALL
                     MQAAHPVVAAGVAQHSTYATDPFGRLMRTLQSTFSVVFGTRSEAEATIRRINAIHASV
                     RGTRPDTGTAYSALEPEPLLWVHATLVDTALRVYERFVAPLTPEERQAYHEESARVAV
                     LLGVPPAIIPPTLVELRAWMARLLEDGRVHVTPAARDIAGTVLHPMPRTPRLAWEAAH
                     LVSVSTLPPVLRQQYRLRWSRARERGVERMAALSRLVLPRVPGMVRFAPQARAAQRRL
                     RRAA"
     gene            complement(1920..>2168)
                     /locus_tag="VFV59_03825"
     CDS             complement(1920..>2168)
                     /locus_tag="VFV59_03825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5324990.1"
                     /translation="NRVLVLFLRFPVWVPAIVAIGLWAAFQFINGLGAAAVTAETGGV
                     AYMAHIGGFIAGVVAGFFFRGVFREPRHPRGTPASLFR"
BASE COUNT          349 a          753 c          835 g          231 t
ORIGIN      
        1 accagcagca gctcgatgat ccgcggcttg gtcagcgaga tgtaggcgcg cacggtgtcg
       61 ccggcgcccg gctcgtgctc gctatcgggc acggcgtggc gcagttcgga gcgggccagc
      121 cggcggctga cgagggtgag ccagaccgat gcgccccaca gggcggcgcc gaccgccagg
      181 tgcggcacga cgaagaaggc cgagaggcgc gacacgacgt tgaggccgcc cagcgcgatc
      241 tggatcccga tgagcacgac catcgtgacg gccaggcggt gggccagcgg cgacagggtg
      301 gagcgatgca cgcgcaccgc gctccacagg accatcaccg ccaccagcac gctcatggcg
      361 cggtgcgcga actggaccgc ctgctcgctg ccgaagatgc gcgggatgac ctgcccattc
      421 atgagcggga agtcggcata ggcgaggccg gcgtggtggc ccgtgaccca ggagccgagc
      481 agcatctggg cgaagatgac cagcgccgtg agcgcgagca ggcgggtcag tcccggccgc
      541 gcgtgcgcgg ccggcatcgg tccatggacg gcccgttcgg cgatcaggat gaccgccgcg
      601 aagacggtga gcgccatgcc gaggtggatg gtcacgagct cggcggcgag gccctggagc
      661 acgaccgccc cgccgatgac cgactggacg atgaccagca cgcccgcgac gacggcggcc
      721 cacagcagcg ggcggtcccg ccgacgctcg gtgaagaccg tgatcaggcc aacggcgccg
      781 accagcggcc cgacgaacac cgccgcgatc agccgatgcg tgtgctcgat ccaggcgtgc
      841 aggtcggccg gcgggatcca gtcgccgaag cacagcggcc aatccgggca gccgaggccg
      901 gagtccgtcg cgcgcaccag gccgccggcg gcgatgaggg cgaacgtggc gacggcagcg
      961 gcgatcgcga gcttggcaaa tcgggtcaaa tcggcagttc ctcgaaccgc ccgatggcac
     1021 gcaggcgggg tcgggccatg atagcgccgc gcctcgcgaa ggccagccgg cggtctcggg
     1081 ctaggctgcg cgacggaggc gccgctgtgc ggcgcgcgcc tgcggggcga accgcaccat
     1141 ccccggcacg cgcggcagca cgagccggct gagcgcggcc atccgctcga cgccacgctc
     1201 gcgggcccgc gaccagcgca gtcggtactg ctggcggagc actggcggca gcgtcgacac
     1261 cgatacgagg tgggccgcct cccatgccag tcgtggcgtt cgcggcatgg ggtgcaggac
     1321 cgtgccggcg atgtcgcgtg cggccggtgt gacgtggacg cggccatcct cgagcagccg
     1381 cgccatccag gcgcgcagct cgacgagggt gggcgggatg atggctggcg gcacgcccag
     1441 caggaccgcc acgcgggccg actcctcgtg gtacgcctgg cgctcctccg gggtcagcgg
     1501 cgccacgaag cgctcgtaca cccgcagcgc ggtgtcgacc agcgtggcgt ggacccagag
     1561 cagcggctcc ggctccagcg ccgagtaggc ggtgccggtg tcgggccgcg tcccgcggac
     1621 cgaggcgtgg atggcattga tgcggcggat ggtcgcctcc gcctccgaac gcgtgccgaa
     1681 gacgaccgag aaggtgctct gcagcgtccg catcagccga ccgaaggggt cggtggcgta
     1741 ggtcgagtgc tgcgcgacgc ccgcggccac caccgggtga gcggcctgca tcagcagcgc
     1801 gcaggagccc ccggccagga cgaccagctc gcggtcgatg cgccacgaga cgctgccggg
     1861 gccgaacagg ccctcggcct cggccagcgc ggcgtgcgcg tcagcgcgcg gcgcgtcggt
     1921 caccggaaga gggaggcagg cgttcctcgc gggtggcgcg gctcccggaa gacgccgcgg
     1981 aagaagaacc cggccaccac accggcgatg aagccgccga tgtgggccat gtacgccacg
     2041 ccgccggtct cggcggtcac cgccgcggcg ccgagtccgt tgatgaactg gaaggcggcc
     2101 cacagcccga tggccacgat ggcgggcacc cacaccggga agcgcaggaa gaggacgagc
     2161 acgcggtt
//
LOCUS       DASTYP010000079         4738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_224362, whole genome shotgun sequence.
ACCESSION   DASTYP010000079 DASTYP010000000
VERSION     DASTYP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224362"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFV59_03830"
     CDS             <1..189
                     /locus_tag="VFV59_03830"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU5324991.1"
                     /translation="HHDLIESLAGAIARAVLDATDAPAVASVEVRVRKPKAPLTGAFE
                     TVEVSLLRHREPGGGEPG"
     gene            complement(186..1478)
                     /locus_tag="VFV59_03835"
     CDS             complement(186..1478)
                     /locus_tag="VFV59_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5324992.1"
                     /translation="MTDDPNRYRGSDPTQPVEVQRYGPPNHNEPARPTWNAAPAWSSR
                     PAAPPPAEPAVRRGLGAVPIIAIAIVAGVLSGGLSAVGVTNLLREPASSAAPLASDQG
                     TGTDKVSDVRIDESSAVITAVDKVAPAVVTIRTQSGGVFGGASGTGSGVIYDANGWIL
                     TNRHVVEDAQALSIELNDGRVFDGTTYGIDTYTDLAIVKIDGSGLPTAPIGTSDGLEP
                     GQLAIAIGNPLGYENTVTTGIVSGLGRQIQASDAAQTSAETLRNLIQTDAAINPGNSG
                     GPLVNSAGQVIGINTAVSTSAQGLGFAIPIDVAKPIMQQALDGKELARPWIGVYYVAI
                     TPTLAQEQSLPAEYGALLETQNGQDPIFPESPAAEAGLKAGDVIVAVDGEQITADRDL
                     STLILPHEPGDTVTLRVLRGNSAQEVQVTLGQLPADAG"
     gene            complement(1525..3066)
                     /locus_tag="VFV59_03840"
     CDS             complement(1525..3066)
                     /locus_tag="VFV59_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5324993.1"
                     /translation="MDLRGRIQRLMQQLNVRLVFALWSVALVGLIVSALAINQILPDY
                     FRQQARERIESAALSVALVTQEIADAQRELNRNALVPELRRIWIFDQVVELAAGPLIP
                     ATVAIYEPAPIGLQSEATPDDTASLQAEGLNLDSEIGPFDSGLIPLRITAEGETVPYR
                     IVISDPWTSREATLDQIRSVIVGAGIVALVVSLLLGVLVARRITAPIYRLRRVAGHVA
                     LGNIDERATASGVLEVDELAAQFNVMADRLAGTLRMLEADRDRLREFVADVSHELRTP
                     IAALRMYTELQLDGGTDEPTRHEFLERSTEQIRRLEWLSTNLLDLSRIDAGIFPLDMR
                     TGDLRDPIQAVVQALSEVADERGVALESVVPGQAVELRFDRERIVQLLTNLVGNGLKF
                     TPRGGTVKVELTDAPEGATIEVRDTGPGIPEEELPHIFERFYRGTNTGDARASGSGLG
                     LAIVRSIVEMHAGEVDVASVIGQGTAFRITLPRTPEPAEAEAEEKVNETSRDHRPARN
                     PGSIA"
     gene            complement(3095..3793)
                     /locus_tag="VFV59_03845"
     CDS             complement(3095..3793)
                     /locus_tag="VFV59_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5324994.1"
                     /translation="MAGTIMIVEDEPAVARGVQVALEREGYAVSVQPTGEDAVAQFAD
                     VAPDLVVLDVRLPGMDGFEVLRQLRRETRAPVLFLTARSDEVDRVVGLEMGADDYLVK
                     PFSIRELVSRIKALLRRAYGELADTQTGRTLRRFDLVIDLERRRVTRDGERIGLTTTE
                     FDLLRHLAARPGRVYTRAQLLELVRDYDDALEQDERTINVHISHIRDKIEPDPSQPRY
                     IRTVRGVGYAFAED"
     gene            4002..4691
                     /locus_tag="VFV59_03850"
     CDS             4002..4691
                     /locus_tag="VFV59_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU5324995.1"
                     /translation="MDLELTLEAREAQGKANKRLRRAGMVPGVVFGKGEDSVPVQVEA
                     KTFETLYRSAGRTSVVKFRLPGASRAKSGIIKSVQRHPLTGSALHVDYFLVNLRQEME
                     IEVPIVYTGEAPAVELTGGTLLHNLSSVVVRALPNDIPHELSIDVSSLQTLDDAIKVA
                     DLPIDTEKVHFVTDPETVIATVVPPRVEVEEEVVPEEEAEGEAEAAAEAGGAEGAPER
                     EESGEEAGSES"
BASE COUNT          662 a         1626 c         1718 g          732 t
ORIGIN      
        1 caccacgacc tgatcgagtc gctggccggc gccatcgcgc gtgccgtgct cgacgccacg
       61 gacgcgccgg cggtggcgag cgtcgaggta cgggtgcgaa agccgaaggc cccgctgacc
      121 ggggccttcg agacggtgga ggtgtcgctg ctccggcatc gggagccggg cggcggggag
      181 ccgggctagc cggcgtcggc gggcagctgt cccagggtga cctgtacctc ctgcgcggag
      241 ttgccgcgca gcacgcgcag ggtcacggtg tcgcccggct cgtgcggcag gatcagggtc
      301 gagaggtcgc ggtccgcggt gatctgctcg ccgtcgaccg ccacgatgac gtcgccggcc
      361 ttcaggcccg cctccgccgc cgggctctcc gggaagatcg gatcctgccc gttctgggtc
      421 tcgagcagcg cgccgtactc ggccggcagg ctctgctcct gggccagggt gggggtgatg
      481 gcgacgtagt acacgccgat ccagggtcgc gcgagctcct tcccgtcgag cgcctgctgc
      541 atgatcggct tggccacgtc gatcgggatc gcgaagccca ggccctgcgc gctggtgctg
      601 accgcggtgt tgatgccgat gacctgcccg gcactgttga ccagcggacc gcccgagttg
      661 ccgggattga tggccgcatc ggtctggatc aggttgcgca gggtctcggc gctggtctgc
      721 gccgcgtcgg aggcctggat ctgccggccg aggccggaga cgatgccggt cgtgacggtg
      781 ttctcatagc cgagcgggtt gccgatggcg atcgccagct ggccgggctc gaggccgtcg
      841 ctggtcccga tgggcgcggt cggcaggccg ctgccgtcga tcttcacgat ggccaggtcg
      901 gtgtaggtgt cgatgccgta cgtcgtgccg tcgaagacgc ggccgtcatt cagctcgatg
      961 ctgagggcct gggcatcctc cacgacgtgc cggttggtga ggatccagcc gttggcgtcg
     1021 tagatgacgc cggagccggt gccgctggcg ccgccgaaga cgccgccgct ctgggtgcgg
     1081 atggtgacga ccgccggggc gaccttgtcg accgccgtga tgaccgcgct cgactcgtcg
     1141 atgcggacat cactgacctt gtcggtgccc gtgccctggt ccgaggccag gggcgccgcc
     1201 gacgaggcgg gctcccgcag caggttcgtc acgccgaccg ccgagaggcc gccggacaga
     1261 acgccggcga cgatcgcgat ggcgatgatg gggacggcac cgaggccgcg ccgcacggcc
     1321 ggctcggccg gtggaggcgc cgcggggcgg ctcgaccagg cgggggccgc gttccaggtc
     1381 ggtcgggcgg gctcgttgtg gttgggggga ccgtagcgct gcacctcgac cggctgggtc
     1441 gggtcgctgc cgcggtatcg gttcgggtcg tcggtcatcg cttcgtgcct gtgcttcgtt
     1501 ggtgcctcgc cgggcgggca ccgatcacgc tatggaaccc ggattgcgcg caggccgatg
     1561 gtcgcgtgaa gtttcgttga ccttctcctc cgcctccgcc tcggcgggct ccggcgttcg
     1621 gggcagggta atgcggaacg cggttccctg cccgatgacg ctcgccacgt cgacctcgcc
     1681 ggcatgcatc tccacgatcg accgcacgat cgccagtccg aggccgctcc cggacgctcg
     1741 ggcatcgccg gtgttcgtgc cgcggtagaa ccgctcgaag atgtgcggca gctcctcctc
     1801 ggggatcccc gggcccgtgt cgcggacctc gatggtggcg ccctcgggcg catcggtcag
     1861 ctcgaccttc acggtgcccc cgcgcggcgt gaacttcagg ccgttgccga ccaggttggt
     1921 cagcagctgc acgatccgct cgcggtcgaa gcgcagctcg acggcctgcc ccggcacgac
     1981 gctctccagg gccacccctc gttcgtcggc gacctccgag agggcctgca cgacggcttg
     2041 gatcgggtcg cgcaggtcgc cggtccgcat gtcgaggggg aagatgccgg cgtcgattcg
     2101 cgacaggtcc agcaggttgg tgctcagcca ctccagccgc ctgatctgct cggtcgaccg
     2161 ctccaggaac tcgtggcggg tgggctcgtc cgtgccgccg tcgagctgca gctcggtgta
     2221 catgcgcagc gccgcgatcg gcgtccgcag ctcgtggctg acgtcggcca cgaactcacg
     2281 cagccgatcc cgatccgctt ccagcatgcg gagcgtgccg gccagacggt cggccatgac
     2341 gttgaactgg gccgcgagct cgtcgacctc cagcaccccc gaggcggtcg cccgctcgtc
     2401 gatgttaccc agcgcgacgt gcccggccac tcggcgcaag cggtagatcg gtgcggtgat
     2461 gcgccgggcg accaggacgc ccagcagcag cgagacgacc agcgccacga tgccggcgcc
     2521 gacgatcacc gagcggatct ggtcgagcgt cgcctcgcgg ctggtccacg ggtcggagat
     2581 cacgatgcgg tatggcacgg tctccccctc ggccgtgatg cgcaggggga tcagccccga
     2641 gtcgaacggc ccgatctcgg aatccaggtt caatccctcc gcctgcagcg aggccgtgtc
     2701 gtccggcgtc gcctccgatt gcaggccgat cggcgccggc tcgtagatgg ccacggtcgc
     2761 cgggatcagg ggaccggcgg cgagctccac gacctggtcg aagatccaga tgcgccgcag
     2821 ctcgggaacc agcgcgttgc ggttcagctc gcgctgggca tcggcgatct cctgcgtgac
     2881 cagcgccacc gacagcgccg ccgactcgat gcgctcgcgg gcctgctgcc ggaagtagtc
     2941 gggcaggatc tggttgatgg ccagggccga cacgatgagg ccgacgagtg ccaccgacca
     3001 gagagcgaag accaggcgga cgttgagctg ctgcatgagg cgctggatgc gcccccgcag
     3061 gtccatgctc ggcagccgat gctgcgccgg gacgtcagtc ctcggcgaag gcatacccga
     3121 cgcccctcac cgtccggatg tagcggggct gcgacggatc gggctcgatc ttgtcgcgga
     3181 tgtggctgat gtggacgttg atcgtccgct cgtcctgctc cagcgcgtcg tcgtagtcgc
     3241 ggaccagctc cagcagctga gcgcgggtgt agacgcgacc cggccgcgcg gccaggtgcc
     3301 ggaggagatc gaactcggtg gtcgtcagac cgatgcgctc gccgtcgcgc gtcacccgtc
     3361 gccgctcgag gtcgatgacc aggtcgaatc ggcggagcgt tcggcccgtc tgcgtgtccg
     3421 ccagctcccc gtacgcgcgg cgcagcaacg ccttgatgcg gctgacgagc tcgcggatgc
     3481 tgaacggctt gaccaggtag tcgtcggcac ccatctccag gcccacgacc cggtcgacct
     3541 cgtcggagcg cgcggtgagg aagaggaccg gcgcgcgcgt ctcgcggcgc agctgtcgca
     3601 ggacctcgaa cccgtccatg cccgggaggc gaacgtccag caccacgagg tcgggagcga
     3661 cgtcggcgaa ctgcgccacc gcgtcctcgc ccgtcggctg cacgctgacc gcgtatccct
     3721 cgcgttccag cgccacctgc acgccgcggg cgaccgcggg ctcgtcctcg acgatcatga
     3781 tcgtgccggc catcggctca gtataggaac gatcgctttc ggacccgttg cgggtcgctg
     3841 aagaagcgtt aacgggttgc ccgcgcttgc ccgcccgatc ggcccgcggt accatgccgg
     3901 ccgcaccccg actgcatgaa ccgctgatcg gtgctgcccg tcacttcgcc ggatcgcgag
     3961 tgcccgaggc gcccgacgta cgagagagga tcagttcacc catggacctg gagctcaccc
     4021 ttgaggcgcg cgaggcgcag gggaaggcca acaagcggct gcgccgtgcc ggcatggtgc
     4081 cgggcgtcgt cttcggcaag ggcgaggact cggtcccggt ccaggtcgag gccaagacct
     4141 tcgagaccct ctaccgttcc gccggccgga cgagcgtggt gaagttccgc ctgcccggcg
     4201 cgagccgagc caagagcggg atcatcaaga gcgtccagcg ccatccgctc acggggagcg
     4261 cgctgcacgt cgattacttc ctggtcaacc tccgccagga gatggagatc gaggtgccga
     4321 tcgtctacac gggcgaggca ccggccgtcg agctgaccgg cgggaccctg ctccacaacc
     4381 tctcgtcggt ggttgtccgg gcgctgccca acgacattcc gcatgagctc tcgatcgacg
     4441 tctcgagcct ccagacgctg gacgacgcga tcaaggtcgc cgatctgccg atcgataccg
     4501 agaaggtcca cttcgtcacc gaccccgaga cggtcatcgc gaccgtcgtt ccgccgcgcg
     4561 tcgaggtcga ggaggaggtc gtgccagagg aggaggccga gggcgaggcc gaggcggctg
     4621 ccgaggccgg tggtgccgag ggcgctccgg agcgcgagga gagcggcgag gaggccggct
     4681 ccgagtcgta actgtggcgc agcgtcgccg cttggtccac gcgcaccgca acggcacg
//
LOCUS       DASTYP010000080         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1026106, whole genome shotgun sequence.
ACCESSION   DASTYP010000080 DASTYP010000000
VERSION     DASTYP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026106"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFV59_03855"
     CDS             complement(<1..372)
                     /locus_tag="VFV59_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5324996.1"
                     /translation="MRSPALRAARVGDHLPPSIVDRARARRLDATALFVPGAGGGTAY
                     GDQLEAHRDDFAAWDGRVIVDEPDGEATHRLLIVDRYGQVYAVHDEADVGALPTPHDL
                     EEWFRFLATACPECGVLDEALL"
     gene            complement(387..788)
                     /locus_tag="VFV59_03860"
     CDS             complement(387..788)
                     /locus_tag="VFV59_03860"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEU5324997.1"
                     /translation="MGLDNLNLLGAIAGAIGAMALGSLWYSPFLFGNRWQELIGIAPD
                     EMTSPVAAMGVAAIMFVLYGVGLGWIIPDGANVGIGLMWGFLAFWAFALPATVVNGVF
                     ERRSWNLMAIYLGYLLLSSLLIAAIVTFLGG"
     gene            complement(866..1798)
                     /locus_tag="VFV59_03865"
     CDS             complement(866..1798)
                     /locus_tag="VFV59_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5324998.1"
                     /translation="MTTTAANATPRTAVARADDRAAQLMLIGMAVCFGGTWVAADIAV
                     AAAPPFTIAAVRFGVASVLLFAWAKVAGRPLSTIRRADWPMILGLGLTAVAGYNWLFL
                     TGLTLAPASDGAIIVPGLAPVFTALIASLALDERIGRRGFIGLAIAAFGLVLVVGPAG
                     AADGTRLLGDGLFLAGAVLWGVYSVLARVASRRFNAVSTTLYGTAVGTLVLLPLAALE
                     NGADALTTAPMDALAGIGYLAVFGTVGAFVLLNLGVARIGAARASAYALLVPIIGVLS
                     SVVLLHDEIGPLTVIGGAIVLVGLWLVEHRRTAN"
BASE COUNT          347 a          772 c          809 g          292 t
ORIGIN      
        1 caggagcgcc tcgtcgagga cgccgcactc cgggcacgcg gtggccagga agcggaacca
       61 ctcttccagg tcgtgcggcg tggggagtgc cccgacgtcg gcctcgtcgt ggaccgcgta
      121 cacctgccca tagcggtcga cgatcaggag ccgatgcgtc gcctcgccat cgggctcgtc
      181 cacgatcacc cgcccgtccc aggcggcgaa gtcatcacgg tgtgcttcga gctgatcgcc
      241 gtaggcggtc ccgccaccag caccgggaac gaagagcgcg gtcgcgtcca ggcgtcgtgc
      301 gcgagcgcgg tccacgatcg agggcggcag gtggtcgccc acgcgagccg cgcgaagtgc
      361 cggcgagcgc atcggtcagg ctgctcctac ccgcccagga aggtgacgat ggccgcgatc
      421 aggaggctgc tcagcagcag gtagccgagg tagatcgcca tcaggttcca ggagcgtcgc
      481 tcgaacacgc cgttgaccac ggtcgcgggg agcgcgaagg cccagaaggc caggaagccc
      541 cacatcaggc cgatcccgac gttcgcgccg tccgggatga tccaccccag ccccacgccg
      601 tacagcacga acatgatggc cgcgacgccc atggccgcga ccggactggt catctcgtcg
      661 ggcgcgatgc cgatgagctc ctgccagcgg ttgccgaaca ggaacgggga gtaccagagc
      721 gatccgaggg ccatggcgcc gatggccccg gcgatggccc ccagcaggtt caggttgtcg
      781 agacccatcg cgtgctcgtc ctccgtggcg tgtgctcgtc acgggcagcg tagccgtcgc
      841 gcgcctccgg ggtcggcgcc gtgggtcagt tcgccgtgcg gcgatgctcc accagccaca
      901 gcccgacgag gacgatcgcg ccaccgatca cggtgagcgg gccgatctca tcgtgcagca
      961 gcacgaccga ggagagaacg ccgatgatcg ggacgagcag cgcgtacgcg ctggcacgcg
     1021 ccgcaccgat gcgcgccacg ccgaggttga gcagcacgaa ggcgccgacg gtgccgaaca
     1081 ccgccaggta gccgatgccg gcgagggcat ccattggcgc tgtggtgagc gcgtccgcgc
     1141 cgttttccag ggccgccagg gggagcagca ccagcgtgcc gacggcggtt ccgtagagcg
     1201 tggtgctgac cgcgttgaac cggcggctgg cgacgcgcgc cagcaccgag tagacgcccc
     1261 acagcaccgc accggccagg aagaggccgt cgccgaggag gcgggttccg tccgccgcgc
     1321 cggccggccc gacgaccagc accagcccga aggcggcgat ggccagcccg atgaagccac
     1381 gccggccgat ccgttcgtcc agcgccaggc tggcgatgag cgcggtgaag accggcgcca
     1441 ggccgggcac gatgatcgcc ccgtccgatg ccggcgcgag ggtcagcccg gtcaggaaga
     1501 gccagttgta cccggccacc gcggtcagtc cgaggccgag gatcatcggc cagtccgcgc
     1561 gcctgatggt cgagagcggg cgtccggcca ccttcgccca cgcgaagagc aggaccgagg
     1621 cgacgccgaa ccgcaccgcc gcgatggtga acgggggcgc ggcggcgacc gcgatgtcgg
     1681 ccgccaccca ggtcccgccg aagcagacgg ccatgccgat cagcatcagc tgggccgcgc
     1741 ggtcgtcggc ccgcgccacg gcggttcgcg gcgtcgcatt cgccgcggtg gtggtcatcg
     1801 gcgcctggtt ctccaagtgg tccatggtcc gtgcgtcagt cgcccgcgtc atcggtgaaa
     1861 cgacgtagcg gcgtcgcccg tgaggtcacg ggctgccctc actgtcgtgg acaggctgtg
     1921 gatagctgcg tgctggcggc gaaaagtcgg ggataagtgg gattgcgtct cgcctcgggc
     1981 ggcgggcata ctgtggtcgt cccgaagggg caagcgcacc cggatcacac gatttcgatc
     2041 atgagttccg gggaccggca ccggcggctt cggccgaacg gaccgggcgg gtacggtggc
     2101 ttcggccatc ggcactcgag gcggttgttc cttcgggatt tccgttctgc gcgcgtggcg
     2161 ccgtggcgcg tcccaccgcc cttcccggcc cttgggcagc tggcggcctg gcccgcgccg
//
LOCUS       DASTYP010000081         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_406290, whole genome shotgun sequence.
ACCESSION   DASTYP010000081 DASTYP010000000
VERSION     DASTYP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406290"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFV59_03870"
     CDS             complement(<1..620)
                     /locus_tag="VFV59_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5324999.1"
                     /translation="MTPSGSPWSADRRLLTVGLIGLVTAGAFEGMAVPTVLPALVDDL
                     GGLDLYGWAFSAFWLTNIIGITLGGADADRYGPRRALLAGSLLFAAGMMVAGLAASMP
                     VVIAGRAIQGFGSGAIASVVYAVIARAYAPSAAPRMIALTSSAWIVPGLIGPALAGAV
                     SDAIGWRWVFLGIVPAVVAMGVVVSPQLRQLGPVVRETMPSRGYGRR"
     gene            complement(617..1792)
                     /locus_tag="VFV59_03875"
     CDS             complement(617..1792)
                     /locus_tag="VFV59_03875"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU5325000.1"
                     /translation="MKRIGILTGGGDVPGLNSVIKSVVYRSSEFGWQSVGFRRGWKGL
                     THVDTAREDGGGYLRPLDRVTTRAIDRTGGTVLHTSRTNPSSMTRTSLPPGLGEDRIG
                     GMQTGEDRYDITPYVLENLEKLGITALVAIGGDDTLSYAARLGREGFPVIAIPKTMDN
                     DVHGTEYCIGFSTAVTRAKELINRQRTTLGSHERIGVFRIFGRNAGYTAWYAAYVTSA
                     RCVIPEAPFDLDALAGVLAEDWRLNPSNYAFVIASEGAIWQGGQIGEVGEADMFGHRH
                     KADVGESLAAELKKRTGIETVNSDLTYDLRSGDPDAIDQLVAITFANIAVDLLHDGTF
                     GRMVAVRDGNYAHAPLPERSLGARQLDVPTMYNVERFRPRYEAKLGAPLLLGPAVTE"
     gene            1906..2097
                     /locus_tag="VFV59_03880"
     CDS             1906..2097
                     /locus_tag="VFV59_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325001.1"
                     /translation="MSSTPTRERRYVTWRLPDRHFSKVHLIGEPGSTVCGRALPRATG
                     QPKAPIPDSEMCPDCDAQG"
     gene            2118..>2515
                     /locus_tag="VFV59_03885"
     CDS             2118..>2515
                     /locus_tag="VFV59_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5325002.1"
                     /translation="MAPQPTADDAGGRFVFLPIPGDALVVLVGVAGSGKSTFAHRHFA
                     PTQVLSSDQLRSLITDDPAAQAATDDAFDLLHRILAMRLRRGRLTVVDATNVEAWARG
                     ELVAVARRHRRPAVAIVLDLPLPEVLRRNL"
BASE COUNT          369 a          909 c          857 g          380 t
ORIGIN      
        1 cggcgcccgt acccccggga cggcatcgtc tcgcgcacca ccgggccgag ttgccgcagc
       61 tgcggcgaga ccacgacgcc catggcgacg accgcgggga cgatgcccag gaacacccag
      121 cgccagccga tggcgtccga caccgcgccg gccagtgccg gcccgatgag gccgggcacg
      181 atccaggcac tggaggtcag cgcgatcatc cgcggagcag ccgagggcgc ataggcgcgc
      241 gcgatcacgg cgtataccac cgaggcgatt gcgccggacc cgaacccctg gatcgcgcgc
      301 ccggcgatga ccactggcat cgaggccgcc agcccggcga ccatcatgcc ggccgcaaag
      361 aggagcgaac cggccaggag cgcgcgacgc ggaccgtatc ggtccgcatc ggctccgccc
      421 agggtgatgc cgatgatgtt cgtcagccag aaggcgctga aggcccaccc gtacaggtcg
      481 agcccgccga ggtcgtcgac cagggcgggc aggacggtgg ggaccgccat cccctcgaag
      541 gcaccggcgg tgacgagccc gatgagcccg acggtgagca gccggcggtc cgcggaccac
      601 gggctgcccg acggcgtcac tcggtgaccg ccggacccag gagcagcggc gcccccagct
      661 tcgcctcgta gcgcgggcgg aagcgctcga cgttgtacat cgtcgggacg tcgagctgcc
      721 gcgcgcccag cgatcgctcc ggcagcgggg cgtgggcgta gttcccatcg cgcacggcga
      781 ccatgcgtcc gaacgtcccg tcgtgcagca ggtccacggc gatgttcgcg aaggtgatgg
      841 cgaccagctg gtcgatcgca tcggggtcgc cggaccgcag gtcgtaggtc aggtccgagt
      901 tcaccgtctc gatgcccgtc cgcttcttga gctcggcggc cagcgactcg ccgacatcgg
      961 ccttgtgacg gtggccgaac atatcggcct cgccgacctc gccgatctgc ccgccctgcc
     1021 agatggcgcc ctcgctggcg atgacgaagg cgtagttcga cgggttcagc cgccaatcct
     1081 ccgccagcac gccggccagc gcgtccaggt cgaagggcgc ctcggggatg acgcagcggg
     1141 cgctcgtcac gtaggcggcg taccaggccg tgtagccggc gttgcggccg aagatgcgga
     1201 agacgccgat gcgctcgtgg ctccccaggg tcgtgcgctg gcgattgatg agctccttcg
     1261 cccgcgtgac cgcagtcgag aagccgatgc agtactcggt cccgtggacg tcgttgtcca
     1321 tggtcttggg gatggcgatg accgggaagc cctcgcgccc cagtcgggcc gcgtagctga
     1381 gggtgtcgtc gccgccgatg gccaccagcg cggtgatgcc gagcttctcc aggttctcga
     1441 ggacgtaggg agtgatgtcg taccgatcct cgcccgtctg catgccgccg atgcggtcct
     1501 ccccgagccc cggcggaagg ctggtccgtg tcatggacga ggggttggtg cgcgaggtgt
     1561 gcagcacggt cccgccggtc cgatcgatgg cgcgcgtggt gacccggtcc agcggacgca
     1621 ggtagccacc gccgtcctcg cgggccgtgt cgacgtgcgt cagtcccttc catccgcgcc
     1681 ggaatccgac gctctgccag ccgaactcgc tggaccgata gacgacgctc ttgatgaccg
     1741 aattgaggcc ggggacgtcg ccgccaccgg tcaggatgcc gatgcgcttc atgctcgctt
     1801 cgtgctcgtt ctcacacgtg ttttggtcat gctgacacac catctggggt ttgtggaaag
     1861 ggcggatttc tggcacaatt acgatccgca aacggagttc cgcccttgtc ctcgacccct
     1921 actcgtgagc gccgatacgt gacgtggcgg ctgcctgatc gccacttcag caaggtccat
     1981 ctcatcggcg agcccggctc cacggtctgc ggccgcgccc ttccgcgcgc caccggccag
     2041 ccgaaggccc cgattcccga ctcggagatg tgcccggact gcgacgcgca gggctgacac
     2101 gccccttcag cgccacgatg gcaccccaac cgaccgccga cgatgccggc ggtcggttcg
     2161 tcttcctccc gattccgggc gacgccctgg tggtgctcgt cggcgtggct ggcagtggca
     2221 agtccacctt cgcccatcgc cacttcgcgc ccacccaggt cctctcgtcc gatcagcttc
     2281 gctccctcat caccgatgat ccggccgccc aggccgccac ggacgacgcc ttcgacctgc
     2341 tgcaccggat cctggccatg cgcctccgcc gcggccggct gacggtggtc gacgccacga
     2401 acgtcgaggc ctgggcgcgc ggcgagctcg tggccgtcgc gcgtcgccac cggcgtcccg
     2461 cggtggcgat cgtcctggac ctcccgctgc ccgaggtgct gcgccgcaac ctcga
//
LOCUS       DASTYP010000082        13341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_807359, whole genome shotgun sequence.
ACCESSION   DASTYP010000082 DASTYP010000000
VERSION     DASTYP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13341
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807359"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFV59_03890"
     CDS             complement(<1..482)
                     /locus_tag="VFV59_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325003.1"
                     /translation="MILPRQPDPRLVTIRRGGLLTDADHALLAMWAADCAGHVLPLFE
                     LARPDDDRPRRAIELARAWASGEVRMTVARTAAGHANGAARGLRGAPRFAAYAAAQAA
                     AVGHVAAHDLGAAAYAIKAVRAAAPRDEADRAGRVECQWQREQLPARVRELVLDDQRR
                     R"
     gene            complement(488..1408)
                     /locus_tag="VFV59_03895"
     CDS             complement(488..1408)
                     /locus_tag="VFV59_03895"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU5325004.1"
                     /translation="MADDVVRAAVYGHLVGDAMGVPYEFTGRDEHRVVEVRGGGAHGQ
                     PPGTWSDDGALMLALLDSLLRRRFDPEHQGQRALAWYDNGAYTPDADGPFDVGTATAA
                     ALARLRAGTAAIDAGGTGEHDQGNGSLMRVLPLALVDRRLADAELVERAHLASRVTHG
                     HPNCQVACALYVLLARTLLEHPGGDPEAALAYSVDRLLAVYQREPASASVLRELLAHR
                     ERVRKHGGGWVLDSFWSAWEAFATSDSYRASIERCIRYGHDTDTTAAIAGGLAGIRWG
                     LDEARGGIPGDWLEALRGRDVVEGLLARIP"
     gene            complement(1401..1667)
                     /locus_tag="VFV59_03900"
     CDS             complement(1401..1667)
                     /locus_tag="VFV59_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325005.1"
                     /translation="MTAACDVPGCEREAIASLPMLRGMTLTIDGIGFQGESFRVCVEH
                     APLVAEKLDDALTGARQRHDARTNFAPGDAEWDRTFDRDRDRDG"
     gene            complement(1783..2247)
                     /locus_tag="VFV59_03905"
     CDS             complement(1783..2247)
                     /locus_tag="VFV59_03905"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5325006.1"
                     /translation="MAHITRSGHYEAPIERVFELATDFQRYPEWNVNYSEIRDVSGPV
                     EIGTVVTGVMQFMGRKVEGAGEIVELDTPYLIRMKGERPEGGRMDMTYRFTPAGTGTD
                     YAFDVDYELPPGIIGQIADRLFVERAVERDLAHTIENFGALVEAPVREPATV"
     gene            complement(2504..4522)
                     /locus_tag="VFV59_03910"
     CDS             complement(2504..4522)
                     /locus_tag="VFV59_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5325007.1"
                     /translation="METQGDPDSDAHARRRPARTPAFALAAMLLLGACGGQPAAPSAS
                     GAAATTAPAATAQAGPTTTPTPGATHVAGGGAGGTDGMAAFSDSRSFTESTWRMLAPI
                     LKSQACTATAREPEEMSREEALDRARKFLSDAVGENAMRAFESSPEASSADAAKTAAA
                     AAAITGNDAGALAAMLAAIGHEPDNPRHLVNAAALMPAFGLGSEALALLDAADGMAAP
                     DSTPYGLNPQAVAQTNRGRALLSLGQWDEAASVLEGALATDGALLEAADNLSLALLCQ
                     GKDDEAMNVARSNRHREKPRTTERDGTTTPNPEDVFEMSLGVDESPLLPNLHIPSSPG
                     EGVALRDQITEIKQDLVDRTIARAQQQMQLQGQLNAAGLHQMTVQRNGEIMAAIAAVS
                     ASEGPAADAWTDVVDASNDAFAEWDAFWGPSGEVESISNGCSNTDDYNGCMRTECIPA
                     TEAFHAAWSNHALALDDLVREWADLYHPIATAIAGHIGNPIQHELAVLSIQGQLDTAF
                     LDVVSAVEMVAMSEDNARDTCVEGFAPVPEDYEPVTPGAGEPCRMNTGQWKLKIAFVS
                     LQAECSDWSIEASTPGPIGVFISVSSKGGATTIFAGPSASASVGPFEAGSKSGFYVRS
                     SPSGTTDFGYRVEPGSTSINAGPVQVEGPQMQAMDFSFVGIGAYLPGM"
     gene            4662..5081
                     /locus_tag="VFV59_03915"
     CDS             4662..5081
                     /locus_tag="VFV59_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325008.1"
                     /translation="MALSLLLLTACSITDRPNGMPDSDPSPPELTLNGVSGMPITWCR
                     GAVSCADGTVDESTDLPAVSPPLELELGDGFQVSSVKAWNGEWPNPRSVDVTMDGSII
                     RDVPDGTEMISVLVAFENWTIDYFWGYTDAASESDPV"
     gene            5096..5656
                     /locus_tag="VFV59_03920"
     CDS             5096..5656
                     /locus_tag="VFV59_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325009.1"
                     /translation="MRTSRVVRVVALTMIVAGCSAFQAPTPAPLPTDVPLFPALMPAS
                     SDVPGIEGWAPAPSEVAPAPPPVEGPDTPPLAAINGVEGVLVSFCWKLGCADGVPGAP
                     DTLPTISPPLDLEIPDGASVAGVTAWNGLQINPRSLEVPFEGTSIGDVPEGTAMLSVF
                     VTFDQGGDAVYYWHFVDPGATGSPTP"
     gene            5768..7378
                     /locus_tag="VFV59_03925"
     CDS             5768..7378
                     /locus_tag="VFV59_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEU5325010.1"
                     /translation="MPVRALAAFPVRASIAVTLAALLSALLLIAATPRSAKASAGTLY
                     GVTGSRSGGCELTEAPPASHLYTIDPANGDATDIGAIHVDGTDVFHVTSLAIHPGTGA
                     MYAIANTQNWANCDDWGTGTLLLIDPTDASAEVVGGWGSMTGGNFPDASFDPWGNLFV
                     WGEGEDRLLAVNTTTGTTSETTPADCSTSETGLAIDSGGFRWITNAGTLTRVHHVSGL
                     CRPWAATGGASNIAAFDANGVLWYGWRNQGVTDIYTYDPEADAGAHEATIALGNLAAI
                     EWDVTGITPGGVADLRVSVEASAASAVIGDPVTFTVTVTNDGPDTATGIEVFHALPSV
                     FSFDNVVASQGLIDPGAPDYWDVGSLAKDGSATVTFSTTAIGGGGRDWLGAEIRNGDQ
                     LDPDSVPWDNDYDVPEDDTALATVTVLDPDLDMAVTDLQVKGKLRFNSKGVGVNVVVT
                     NFGVNAFSFDSASQLRLTWNDQSSRVTCRAVTTLLAPGKSATVRCKVNLLNSGVSPGA
                     TIVFRAELPLQHDFDESNNVRELTVTAS"
     gene            7529..9409
                     /locus_tag="VFV59_03930"
     CDS             7529..9409
                     /locus_tag="VFV59_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325011.1"
                     /translation="MLVDTRTLEEREHGYPPSPATLIRRCTAPICGRPDEWLFNEVHA
                     EVIAYLSRPGLTVDDLFRDYADASGSFAEPSLHDLGPGRFLRHFGLYEDREAAQDLID
                     AHPADALDVIRVERIGPDTFYDLWRMCLVRWIRAQGETLLVLTWGNGDPVDELYFAIT
                     TAGVSSAVPHYVRTRNGLPLAVDTGALFVLPALGDYEIRCAWPTPLFAEEAISYKSNE
                     LVAGTSLARARATPTRAVLRTGVSARILRVFHDEGLDRLAISSTKATQPRNNKDGPYR
                     DATGTECTTPCTGFSYVDQADLENSPVRTLTPHPRRDFATIPDLPAGTYTVSGYGKEA
                     GITVTTTRDVTIGQAQVAEAGMDHTFDLSRLQLPPPRKAFLQGDVVRQSQGSTRWCGP
                     YSLSHAFSYWAPWAFNPTSRNGHWMGDNVHDGWQTWAHIIIAIGTLGIAPTVYSIFED
                     DPSPGTLQETLVRGCSIFGFSAQPYTYDGVSRDEALGHLKRWLAAGVPVLVAVDEYMD
                     QGDGHWSTEHFKVIVGYDDDARLRYTDDNGVDQENVGAFYFINSGGMGEERDDPTDIA
                     AQYRENHADYDNVPIGNDADAYEIFWKKWEAGSIPTFSKNFWCLPIYPVVWDRIPDAE
                     EP"
     gene            9550..10350
                     /locus_tag="VFV59_03935"
     CDS             9550..10350
                     /locus_tag="VFV59_03935"
                     /inference="COORDINATES: protein motif:HMM:NF040373.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6299 family protein"
                     /protein_id="HEU5325012.1"
                     /translation="MKRMAFPSSAVLLFTLVLATPVLAAAPSNDSFAGRTVITALPFS
                     ETLETSEATTEASDAELNTECGAPALDASVWYEFTPATDIYVLVDVSASDYPAGVLVA
                     TGSPGAFGPVVACGPGGVAFPAVAGETYVFLVIDDQSDGGGNGGTLEITVDEAPPPPD
                     IALTVNPVATFDAQTGSATVTGTVSCTDGASAFIDIALTQRVGRFTVRGFGFAEATCD
                     GTAQPWTAEVFGDTGLFKGGQATASVFGQACNFDCGFTAEEVTIRLRR"
     gene            10511..11902
                     /locus_tag="VFV59_03940"
     CDS             10511..11902
                     /locus_tag="VFV59_03940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5325013.1"
                     /translation="MQSPISIPGFAGEVVQPGHPGYDGHREVWNAIIDRRPAVIARCT
                     SAADVAAAVRYGRDAGLEIGVKCGGHSMIGLSVPDGGLMIDLGPMNAVRIDPDQRRAH
                     VQGGSLLRNLDRSAEPHGLATTAGNVGHTGVGGLTLGGGMGWLARQFGLACDNVVAYT
                     VVTADGETIRATAEEHPDLFFGLRGGGGNFGIVTEFEFRLHPIAHTALVVDRYVGLTD
                     ATAALRAWRDLLPDAPRQATLTADATTVAPGNPELPEHLHGRPVVTVGFVWVGDLDAG
                     RRFLQTFRGATGEAVTESISEMRYVEQQGIADEYHHHGKRRYMKGHYLTELSDAAIEA
                     FIGRGVADGPEPNDWTRVPNGGFQAYGGAIGEVGDDEAAFSHRRTLVEWGGATSWLDP
                     AEDSQRIAAARAYGAAMAPFASGVYVNVLTDEGEAGVRRAYDDAKLARLAQIKRRYDP
                     DNVFHLNHNIRPG"
     gene            12171..13226
                     /locus_tag="VFV59_03945"
     CDS             12171..13226
                     /locus_tag="VFV59_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325014.1"
                     /translation="MRRLISILAATVLAAALAPAVIAADPETEGTWALYPRQTYTDDT
                     VTATSTSYRTEVRGAINPDGSSNWPSKRGVIAVQFDLLAAPSVTTTTTRTYDPPVWES
                     VLPDTPYTILRFDPADTLTFADITNLSADYLFTEGDCFGGSLRWDIYVTHDGVEKLIH
                     VYYGTPNGPDQTCSGAASGSGANLITTGVSADRFEVQGGWDTAGAVYRTYADALAYTN
                     SGTDEVLAVQLTLDSGWHANQRADISNITVNDNTWVPKTTETLSSTTITGDFAPTCDL
                     PAAELRWAKNNSSASGAINEALSIQPKDSNGVYRQVDCKYIYNLDVSSLAGAGTYTVW
                     VRIGGELVDDPASFQLR"
BASE COUNT         2048 a         4731 c         4498 g         2064 t
ORIGIN      
        1 cgccggcgct ggtcgtccag caccagctcg cggacgcgcg ccggcagctg ctcgcgctgc
       61 cactggcact cgacccggcc cgctcggtcc gcttcgtcgc gaggcgccgc cgcacggacc
      121 gccttgatgg cgtaggccgc cgcgccgagg tcgtgcgcag caacgtgtcc aacggcggcc
      181 gcctgagcgg cggcgtaggc agcaaaccga ggggcgccac gcagaccgcg agcggcgccg
      241 ttggcgtggc cggcggccgt gcgggctacc gtcatccgca cctcaccgct ggcccatgct
      301 cgggccagct cgatcgcccg acgcgggcgg tcgtcatcgg gccgcgccaa ctcgaagagc
      361 gggaggacgt gcccggcgca gtcggccgcc cacatcgcca ggagggcgtg gtccgcatcg
      421 gtcaggagtc cgcctcggcg gatggtgacc agtcgcgggt cgggttgcct gggaaggatc
      481 acgaccttca gggtatccgg gccagcagac cctccaccac gtcgcgcccg cggagcgcct
      541 cgagccagtc gcccgggatc ccgccacgcg cctcgtccag tccccagcga atcccagcca
      601 ggcctccggc gatggcggcg gttgtatcgg tgtcgtggcc gtaccggata cagcgctcga
      661 tgctcgcccg gtaggagtcg gaggtcgcga aggcctccca tgccgaccag aaggagtcga
      721 gcacccagcc accgccgtgc ttgcgcacgc gctcgcggtg ggccaacagc tcccgcagga
      781 ccgatgcgga cgccggctct cgctggtata ccgccagcag ccgatcgacc gagtacgcca
      841 gggcggcctc cggatcccca ccgggatgct cgagcagcgt acgcgcgagc agcacgtaca
      901 gcgcacacgc gacctggcag ttgggatgtc cgtgcgtcac ccgcgaggcg aggtgcgcgc
      961 gttcgacgag ctccgcgtcc gccaggcgcc gatccaccag cgcgagaggc aggacgcgca
     1021 tgaggctgcc gttgccctgg tcgtgctcgc cggtgcctcc tgcgtcgatg gcggcggtgc
     1081 cagcacgcag ccgggccagc gcggcagcgg ttgccgtgcc gacgtcgaac gggccgtccg
     1141 cgtccggcgt gtaggcgccg ttgtcgtacc aggccagggc ccgctgcccc tggtgctcgg
     1201 gatcgaaccg gcgacgcagc aacgaatcga ggagggcgag catgagcgcg ccatcgtcgc
     1261 tccacgtgcc gggcggctgc ccgtgggcgc cgccgccgcg cacctcgacc acgcggtgct
     1321 cgtctcgccc ggtgaactca tacggcacgc ccatggcgtc cccgacgaga tggccgtaca
     1381 ccgcggcgcg gaccacgtcg tcagccatcg cggtctcggt cgcggtcgaa ggttcggtcc
     1441 cactccgcat cgcccggcgc gaagttggtg cgggcgtcgt ggcgctgccg tgcgccggtc
     1501 agggcgtcat ccagcttctc ggccaccagc ggcgcgtgct cgacgcagac gcggaacgac
     1561 tcgccctgaa agccgatgcc gtcgatggtc agggtcatcc cgcgcagcat cggcagcgac
     1621 gcgatggcct ctcgctcgca gccggggacg tcgcatgcag cggtcatcgg ctgcatcttg
     1681 cgccagtggc gcgacagctc gcgtgtcgca gtcggccgat tcgatgagga agcggccccg
     1741 gcgcaccacc ggggccgctc tggcgaaccg cgaaccggcc ggctagacgg tcgccggctc
     1801 gcggaccggt gcctccacca gggcgccgaa gttctcgatg gtgtgtgcga gatcacgctc
     1861 caccgcgcgc tccacgaaca gtcgatcggc gatctggccg atgatccccg gtggcagctc
     1921 gtagtcgacg tcgaaggcgt agtcggtccc ggtgccggcc ggcgtgaacc gatacgtcat
     1981 gtccatgcgg ccaccctccg gccgctcgcc cttcatgcgg atgaggtacg gggtgtccag
     2041 ctcaacgatc tcgccggctc cctcgacctt gcgccccatg aactgcatca cgccggtgac
     2101 gacggtgccg atctccacgg ggccgctgac gtcgcgaatc tccgagtagt tgacgttcca
     2161 ctccgggtag cgctggaaat cggtggcgag ctcgaagact cgctcgatcg gcgcctcgta
     2221 gtggccgctc ctggtgatgt gtgccattcg gatcctcctc ggctgcctgt gtgccgagct
     2281 gattccgtgt ccacagcggc cggctggacc cctggggccg cggccaacgc agcccggtcg
     2341 caaccccacg acgttcggcg tcgacgcgcg gcgcgacaag tgccggaggt catcggggaa
     2401 tggcgaatgc ccactcccgc cactgcgacg gtgggcacca ccctccagcg catatcgcga
     2461 tatgcgatcc gccgcgacgg ctctaggtcg ccccctcggc cgcctacatg cccggcaggt
     2521 acgccccgat gccgacgaag ctgaagtcca tggcctgcat ctgcgggccc tcgacctgga
     2581 ccggtccggc gttgatggac gtggagccgg gctcgacccg gtagccgaag tccgtggtcc
     2641 cggatggcga ggatcggaca tagaagcccg acttcgagcc ggcctcgaac gggccgaccg
     2701 aggcgctcgc cgatggaccg gcgaagatcg tggtcgcgcc gcccttggag ctgacggaga
     2761 tgaacacgcc gatcgggccg ggcgtggacg cctcgatgct ccagtccgag cactcggcct
     2821 gcagcgagac gaaggcgatc ttgagcttcc actggcccgt gttcatgcgg cacggctcgc
     2881 cggctcccgg cgtcaccggc tcgtaatcct ccggcacggg cgcgaagccc tcgacgcagg
     2941 tgtctcgcgc gttgtcctcc gacatcgcga ccatctcgac cgcactgacc acgtccagga
     3001 acgcggtgtc gagctgcccc tggatcgaga gcaccgccag ctcgtgctgg atcgggttgc
     3061 cgatgtgccc ggcgatggcc gtcgcgatgg ggtggtacag atcggcccac tcgcgcacca
     3121 ggtcgtcgag cgcgagcgcg tggttggacc aggccgcgtg gaacgcctcg gtcgccggga
     3181 tgcactcggt gcgcatgcag ccgttgtagt catcggtgtt gctgcagccg ttcgagatcg
     3241 actcgacctc gccggatggg ccccagaagg catcccattc agcgaaggcg tcgttcgagg
     3301 cgtccacgac atcggtccag gcatcggcag ccggcccctc ggacgcgctc accgcggcga
     3361 tggcggccat gatctcgccg ttgcgctgca cggtcatctg gtgcaagccg gccgcgttga
     3421 gctgcccctg cagctgcatc tgctgctgcg cgcgcgcgat ggtgcgatcg acgaggtcct
     3481 gcttgatctc ggtgatctgg tcgcgcagcg ccaccccctc gcccggcgac gaggggatgt
     3541 gcaggttggg gagcagggga gactcgtcga cccccaggct catctcgaac acgtcctccg
     3601 gattcggcgt ggtcgtgcca tcccgctccg tggtccgtgg cttctcccga tgccggttgg
     3661 agcgcgcgac gttcatcgcc tcgtcgtcct tcccctggca caggagcgcc agcgacaggt
     3721 tgtcggcggc ttcgaggagc gcgccgtcgg tcgccaaggc cccctccagg accgatgcgg
     3781 cctcgtccca ctgccccagg ctgaggagtg cccgcccgcg attggtctgc gccaccgcct
     3841 gcgggttcag cccgtacggc gtgctgtccg gcgcggccat gccgtccgcc gcgtccagca
     3901 gggccagcgc ctcgctgccc aggccgaagg cgggcataag ggccgccgcg ttgaccaggt
     3961 ggcgcgggtt gtcgggctcg tgaccgatgg ccgccagcat cgcggccagc gccccggcgt
     4021 cattgccggt gatggcggcc gcggcggcgg ccgtcttggc ggcatcggcc gagctggcct
     4081 cggggctgga ctcgaaggcg cgcatggcgt tctcgccgac ggcgtcgctg aggaacttgc
     4141 gagcgcggtc gagcgcttcc tcgcggctca tctcctcggg ctcgcgcgcc gtggcggtgc
     4201 aggcctgcga cttcaaaatc ggcgcgagca tgcgccacgt cgattcggtg aaggagcggg
     4261 agtcgctgaa cgcggccatg ccgtcggtgc cacccgcccc gccaccggcc acgtgcgtcg
     4321 caccgggcgt gggggtcgtg gtggggcccg cctgtgcggt cgcagccggt gccgtcgttg
     4381 cggccgctcc actcgccgat ggtgcggccg gctgcccgcc gcatgcgccg agcagcagca
     4441 tggcggccaa tgcgaacgcc ggcgtcctcg ctgggcgccg gcgcgcgtgg gcgtcggagt
     4501 cggggtcccc ctgcgtctcc atcgccgcac atctaagcat ggacgtcaag ccaccggccg
     4561 aacccatgga ctccgccgcc ggcgaccgtc agaatcggcc gacagggagg aggacgccga
     4621 tgtttcatct ggcccgacca tctgggctcc gacgctcggc gttggcgctc agcctcctcc
     4681 tgctgacggc gtgctccatc accgatcggc cgaacggcat gcctgacagc gatccgtcgc
     4741 cgcccgagct caccctgaat ggcgtctccg ggatgccgat cacctggtgt cgcggggccg
     4801 tctcgtgcgc ggacggcacc gtggacgagt cgacggacct gcccgccgtg tctccgccgc
     4861 tcgagctcga gctcggtgat ggcttccagg tctcgagcgt caaggcatgg aacggcgagt
     4921 ggcccaatcc cagatcagtc gacgtgacga tggatggctc gatcatccgc gacgtccccg
     4981 acggcaccga gatgatcagc gttctcgtcg cattcgagaa ctggacaatc gattacttct
     5041 ggggatatac cgacgccgcc agcgaatcgg atcccgtctg agggaagact cgaagatgcg
     5101 cacgtcgcga gtagtccggg tcgtcgcgct caccatgatc gtcgctggct gctcagcctt
     5161 ccaggctccc acgcccgccc cgctgccgac ggacgtgccg ctgttcccgg cgctgatgcc
     5221 cgcatccagc gacgtgccgg gcatcgaggg ttgggcgccg gctccgtccg aggtcgcccc
     5281 cgcgccacca ccggtggagg gccccgatac gccgccgctg gcggccatca acggcgtcga
     5341 aggcgtcctc gtctcgttct gctggaagct cgggtgcgcc gatggcgtcc ccggagcccc
     5401 cgacacgctc cccacgatca gcccgccgct cgacctcgag atcccggacg gcgcatccgt
     5461 cgccggcgtg accgcctgga acgggctgca gatcaacccg cggtcgctgg aggtgccgtt
     5521 cgagggcacg agcatcggcg acgtgccgga agggaccgcc atgctcagcg tcttcgtgac
     5581 gttcgaccag ggcggcgacg ccgtctacta ctggcacttc gtcgacccgg gcgcaacggg
     5641 atcaccgaca ccgtaggcgt cgcctggctc tccggttgac gtgcgtgata cgtttcccgc
     5701 cgctgctctg tctcaggaga tcccacgggg gctgggtgat cttcgcttcg agatggaggt
     5761 tcctggattg cctgtccgag ctctggctgc cttccctgtc cgcgcatcca ttgccgtcac
     5821 gcttgcggcc ctgctgagcg cgcttctgct catcgccgcc acgccgcgct cggcgaaggc
     5881 gtccgctggc accctctacg gcgtcaccgg ctcccgctcc ggcggatgcg agctgactga
     5941 ggcgccgccc gcttcccacc tgtacaccat cgatccagca aacggcgacg ccaccgacat
     6001 cggcgccatc cacgtcgacg ggacggatgt cttccacgtc acgagcctcg cgattcatcc
     6061 gggtacgggc gcgatgtacg ccatcgcgaa cacgcagaac tgggcgaact gcgacgactg
     6121 gggaaccggc acgttgttgc tgatcgaccc cacggacgca tcggccgaag tggttggcgg
     6181 gtgggggagc atgacgggtg gcaacttccc ggatgcgagc ttcgacccgt ggggcaacct
     6241 gttcgtgtgg ggcgaggggg aggacaggct cctggcggtc aacaccacga ccggtaccac
     6301 gtcggagacg acacccgccg attgcagtac gtcggagacc ggtctggcga tcgactcggg
     6361 cggcttccga tggatcacca atgcgggaac gctgacgcgc gtgcatcacg tgtccggtct
     6421 ctgccggcca tgggccgcga ccggtggcgc gagcaacatc gcggcgttcg atgcgaacgg
     6481 cgtcctctgg tatggatggc gcaaccaggg cgtcaccgat atctacacgt atgacccgga
     6541 ggccgatgcg ggcgcgcacg aggcgaccat cgcgctgggg aacctggccg cgatcgagtg
     6601 ggacgtcacg gggatcacgc cgggcggcgt cgccgacctg cgcgtgagcg tcgaggccag
     6661 cgccgccagt gcggtcatcg gtgatccagt gacgttcacg gtgacggtga cgaacgatgg
     6721 tccggacacg gccaccggaa tcgaggtttt ccacgcccta ccctcggtgt tctcgttcga
     6781 caacgtcgtg gcctcgcaag gattgatcga tcccggagcg cccgactact gggacgtggg
     6841 atcgcttgcc aaggatggat cggccaccgt cacgttctcg acgacggcga tcggcggtgg
     6901 cggtcgagac tggcttggcg cggagattcg caacggtgac cagctcgacc cggacagcgt
     6961 gccatgggac aacgactacg acgtgcccga ggacgacacc gctctggcga ccgtcaccgt
     7021 cctggacccg gacctcgaca tggcggtgac ggacctccag gtgaagggca agcttcgctt
     7081 caactcgaag ggcgtcggcg tcaacgtcgt ggtgaccaac ttcggcgtca atgcgttctc
     7141 gttcgacagt gcctcgcagc tgcgcctcac ctggaacgac cagtcgtcgc gcgtgacgtg
     7201 ccgcgcggta accactctgc tcgccccggg caagtcggcc accgtgcgct gcaaggtgaa
     7261 cctcctgaac tccggagtgt ctccgggcgc gacgatcgtg ttcagggcgg agctgcccct
     7321 tcagcatgat ttcgacgagt cgaacaacgt gcgcgagctg acggtcaccg ccagctgacc
     7381 gatgcgtgcc ggctgacggc gagaccgcca gccggcaccc tccgccatcg gtcctgcgtg
     7441 caacaggacc gtcctattga cgcggctctg atgatcgcgg cgacggccgg accatccggc
     7501 gtgaagcgac gcagacgagg cgcgatccat gctggtcgac acgaggaccc tcgaggagcg
     7561 cgagcacggc tatccgccca gcccggcgac gctcatccgc cgctgcaccg caccgatatg
     7621 cggccgcccc gacgagtggc tgttcaacga ggtgcacgcc gaggtcatcg cctacctcag
     7681 ccgccccggg ctgaccgtcg acgacctctt ccgtgactac gccgacgctt ctggctcgtt
     7741 cgccgagccg tccctgcacg acctgggtcc gggccgtttc ctgcgccact tcgggctcta
     7801 cgaggaccgg gaggcggcgc aggacctcat cgacgcgcat ccggctgatg cgctcgacgt
     7861 gatccgcgtg gagcgcatcg gcccggacac gttctacgac ctgtggcgga tgtgcttggt
     7921 gcgctggatc cgggcgcagg gcgagacgct gctggtcctg acgtggggga atggcgaccc
     7981 ggtggatgag ctctacttcg ccatcaccac ggctggcgtt tcgagcgcgg ttccgcacta
     8041 cgttcggacc cgtaatggcc tgccgctggc cgtcgatacg ggcgcgctct tcgtgctgcc
     8101 ggcgctgggc gactacgaga tccgttgtgc ctggccgacg ccgctcttcg cggaggaggc
     8161 catctcctac aagtcgaacg agctggttgc cgggacgtcg ctggcgcggg cccgggcgac
     8221 gccgacccga gccgtcctcc gcaccggcgt cagcgcgcgg atcctccgcg tcttccatga
     8281 cgaggggctc gaccgtctcg cgatttcgtc gaccaaggcc acgcagccgc ggaacaacaa
     8341 ggacgggccg tatcgcgatg cgacgggcac cgagtgcacc acgccctgca ccggcttctc
     8401 gtacgtcgat caggccgatc tggagaattc gccggttcgc acgctgacgc cccacccgcg
     8461 acgcgacttc gccaccatcc cggaccttcc cgccggcacg tacacggtct ccggttacgg
     8521 gaaggaggca ggcatcaccg tcaccaccac gcgcgacgtc acgatcggcc aggcccaggt
     8581 cgccgaggcg gggatggacc acaccttcga cctgtcgcgt ctccagctgc cgccgccgcg
     8641 caaggcgttc ctacagggcg acgtcgtgcg ccagtcccag gggtccacgc gctggtgcgg
     8701 gccgtactcg ctgtcccacg ccttcagcta ctgggcgcca tgggcgttca acccgacctc
     8761 gcgcaacggc cactggatgg gcgacaacgt ccacgacgga tggcagacgt gggcgcacat
     8821 catcatcgcc atcgggaccc tggggatcgc gccgacggtc tacagcatct tcgaggacga
     8881 ccccagcccc ggcacgctcc aggagacgct ggtgcgtggc tgctcgatct tcggcttctc
     8941 ggcccagccg tacacgtacg acggcgtcag ccgtgacgag gcgctgggtc acctgaagcg
     9001 ctggttggcc gccggagtgc cggtgctcgt cgccgtcgac gagtacatgg accagggcga
     9061 cggccactgg tcgaccgagc acttcaaggt catcgtcggc tacgacgacg acgcgcggct
     9121 gcgctacacc gacgacaacg gggtcgacca ggagaacgtc ggcgccttct acttcatcaa
     9181 ttccggcggc atgggggagg agcgcgacga tccgacggac atcgcggcgc agtaccgcga
     9241 gaaccacgcc gactacgaca acgtccccat cggcaatgac gccgatgcgt acgagatctt
     9301 ctggaagaag tgggaggccg gcagcatccc gaccttcagc aagaacttct ggtgcctgcc
     9361 catctacccg gtcgtctggg accgcattcc ggacgcggag gagccgtagt tacggtgaag
     9421 cggctgaacc ccgcagtcgt cccgcagtga ccatcggctg aactggtcac ggacgacagg
     9481 tcagccgaag ggggtgatga ggattcgtca ctggcagcca tccagtccgt agacctcagg
     9541 agtgcaccga tgaagcgcat ggcgttcccg agcagcgcgg tgctgctctt caccctggta
     9601 ctcgcgacgc ccgtcctcgc cgcggcaccg tcgaacgatt cgttcgcggg ccgcaccgtc
     9661 atcacggcgt tgcccttctc ggagaccctc gagaccagcg aggcgacgac ggaggcgtcc
     9721 gacgccgaac tcaacaccga gtgcggcgcc ccggccctcg acgcgtcggt ctggtacgag
     9781 ttcacgccgg cgacggacat ctacgtgctc gtcgacgttt ccgcatcgga ttaccccgcc
     9841 ggcgtcctgg tggcgaccgg ctcgccgggc gccttcggcc cggtcgtcgc ctgtggcccc
     9901 ggcggcgtcg ccttcccggc cgtggccggc gaaacctacg tcttcctggt catcgacgat
     9961 cagagcgacg gtggcggcaa cggtggaacc ctcgagatca cggtcgatga ggctccgccc
    10021 ccgcccgata tcgccctcac cgtaaatccc gtcgctacgt tcgatgcgca gacgggatcg
    10081 gcgacagtca cgggcaccgt gagctgcacc gatggcgcgt ccgcgtttat cgacatcgcg
    10141 ctgacgcagc gcgtcggccg gttcaccgta cggggcttcg gattcgccga ggcgacctgc
    10201 gacgggaccg cgcagccgtg gaccgccgag gtctttggtg ataccggcct cttcaagggc
    10261 ggccaggcga ccgccagcgt cttcggccag gcctgcaact tcgattgcgg cttcaccgcc
    10321 gaggaggtca cgatccgact ccgccgctga cgcggctaag ggcccgccaa ggccgaagtc
    10381 tccggggccc gtctgccgag gcgggccccg ttcattccgt ggcgcaccga tgagtcgcgc
    10441 actggcatcc cgtctgactg atgagaagcg cgtgcgccag tcgcatccgc gcaggcacag
    10501 gaggacgacc atgcagagcc cgatcagcat ccccggcttc gccggcgagg tcgtccagcc
    10561 cggtcaccct gggtacgacg gccatcgcga ggtctggaac gccatcatcg atcggcgccc
    10621 cgccgtcatc gcccggtgca cctccgccgc cgacgtcgcc gcggcggtcc gatacggccg
    10681 cgatgcgggg ctggagatcg gcgtgaagtg cggcggccac agcatgatcg gcctgtccgt
    10741 gcccgatggc ggcctgatga tcgacctggg gcccatgaac gcggttcgga tcgaccctga
    10801 tcagcggcgc gcccacgtcc agggcggctc gctgctccgc aacttggatc ggtccgccga
    10861 gccgcacggc ctcgcgacga ccgcgggcaa cgtgggccac accggcgtcg gcggcctgac
    10921 cctcgggggc ggcatgggct ggctggcgcg ccagttcggc ctagcctgcg acaacgtcgt
    10981 ggcgtacacc gtggtcaccg ccgatggcga gaccatccgc gcgaccgccg aggagcaccc
    11041 ggacctgttc ttcggcctgc gcggtggggg aggcaacttc ggaatcgtga ccgagttcga
    11101 gttccgcctg cacccgatcg cgcacacggc gctggtcgtc gaccgctacg tcggcttgac
    11161 cgatgcgacg gcggcgctcc gcgcctggcg cgacctcctg cccgatgcgc cacgccaggc
    11221 caccctgacc gccgacgcca ccaccgtcgc tcccggcaac ccggagctcc ccgagcacct
    11281 ccacggccgt ccggtggtga ccgtcggatt cgtgtgggtc ggcgatctcg acgctgggcg
    11341 gcgattcctc cagaccttcc gcggcgcgac cggcgaggcg gtgacggaga gcatcagcga
    11401 gatgcgctac gtcgagcagc agggcatcgc ggacgagtac caccaccacg gcaagcgccg
    11461 gtacatgaag ggccactacc tgaccgagct gtcggacgcg gcgatcgagg cgttcatcgg
    11521 tcgaggggtg gcggatgggc ccgagccgaa cgactggacg cgcgtgccga acggtggctt
    11581 ccaggcgtac ggcggcgcga tcggcgaggt gggggacgac gaggccgcct tcagccatcg
    11641 gcgcacgctg gtcgagtggg gtggcgcgac cagctggctc gacccagccg aggattcgca
    11701 gcgcattgcc gccgcccgcg cctacggtgc cgccatggcg cccttcgcga gcggcgtcta
    11761 cgtcaacgtc ctgaccgatg aaggcgaggc cggtgtccgc cgcgcctacg acgacgccaa
    11821 gctcgcgcga ctggcccaga tcaagcgccg atacgacccg gacaacgtct tccacctcaa
    11881 ccacaacatc cgaccgggtt gatcaagggc tgcccgatgc ccgtccatca atccgccgta
    11941 tataccgata tacgcggccg accgtggatg ctcttcctcg tcgtttggtt gcccagcctc
    12001 tggtactttc gagccagtcc tgctacgttc gccgtggacg gggggaagtg cccgcgccgt
    12061 agtcagcgcc cctgagctcc cccgatcacg tccggccgat tccgccggcc cgaaacgcgg
    12121 atgcgtgcga acctgctggt aggggaacgg gatcgcacgg gggaggaacg atgagaaggc
    12181 tcatctccat tcttgccgcg accgtcctgg cggctgccct ggcaccggcg gtgatcgccg
    12241 ccgatccaga gaccgagggc acctgggcgt tgtatccgcg tcagacctac accgatgaca
    12301 ccgtgaccgc cacctcgacc tcataccgga cggaggttcg tggcgccatc aaccctgacg
    12361 gctcgagcaa ctggccctcg aagcgtggcg tgatcgccgt ccagttcgat ctgctcgcag
    12421 cgcccagcgt cacgaccacg acgacccgca cctacgatcc gccggtgtgg gagtcggtcc
    12481 tgcccgacac gccgtacacc atcctgcggt tcgaccccgc ggacaccttg accttcgcgg
    12541 acatcaccaa cctcagcgcc gactacctct tcacggaggg cgactgcttc ggcggttcgc
    12601 tgcggtggga catctatgtc acgcatgacg gcgtggagaa gctgatccac gtgtactacg
    12661 gcacgcccaa cgggccggac cagacctgct cgggagcggc atccggaagc ggcgccaacc
    12721 tgattaccac cggtgtcagc gcagaccgct tcgaggtcca aggtggctgg gacaccgccg
    12781 gtgcggtgta tcggacgtac gcggatgcgc tcgcgtacac caatagcggc accgatgagg
    12841 tcctcgcggt tcagctcacc cttgactccg gctggcatgc caatcagcgc gcggacatct
    12901 cgaacatcac ggtgaacgac aacacctggg tgccgaagac gaccgagacg ctgagctcca
    12961 cgacgatcac aggtgacttc gctccgacgt gtgacctacc ggcagccgag ctgcgctggg
    13021 cgaagaacaa ctcatcggcg agcggtgcca ttaatgaggc gctgagcatc cagccgaagg
    13081 actcgaacgg cgtctatcgg caggttgact gcaagtacat ctacaacctc gacgtcagca
    13141 gccttgctgg tgccgggacg tacaccgttt gggtccgcat cggcggcgag ctcgtggacg
    13201 acccggcgag cttccagctc cgataggaga cgtcacacga cgcgaggggg gccggacctg
    13261 tccggccctc tcgcccaccc agccccgcta cgtcgaaccg ccgagcgccc ctccgccgac
    13321 cgcccgatgt cccacgagcc c
//
LOCUS       DASTYP010000083         5196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_661792, whole genome shotgun sequence.
ACCESSION   DASTYP010000083 DASTYP010000000
VERSION     DASTYP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661792"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFV59_03950"
     CDS             complement(<1..482)
                     /locus_tag="VFV59_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007998842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325015.1"
                     /translation="MTDRPATPRPPYVAVIGDGDPRGPDAHRMLEWAEEVGQHLARGG
                     AVVVTGGLGGVMRAASRGAVGAGGETIGLLPGAEASEANEYVRTPIATGLGVVRNLVV
                     VTAADAVVAVGGRHGTLSEIGLALRMGRQVVALSSWRIESEHRLGGPRVHRVRDPREA
                     A"
     gene            complement(479..1051)
                     /gene="thpR"
                     /locus_tag="VFV59_03955"
     CDS             complement(479..1051)
                     /gene="thpR"
                     /locus_tag="VFV59_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU5325016.1"
                     /translation="MPDASSWRCFVAVALGEELRASLAAAADRWRAHPNTSGLRWTDP
                     DGWHLTLAFLGDTDPGRVPALLAALNRATGGAASAAYPTGGLGAFPSSGRARVLWYGM
                     EDPERHLRRLAAAVRRELGLPPDDRFRAHVTLARARHDPVDLRPFLESAAAPEGVLDV
                     TAIHLMRSHLGRGPARYESLAAVLLGGSST"
     gene            complement(1044..1784)
                     /locus_tag="VFV59_03960"
     CDS             complement(1044..1784)
                     /locus_tag="VFV59_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU5325017.1"
                     /translation="MTDALPPLRAVFLDIGDTVMRPNPSWEHVYALAFEEFGIHVEID
                     RLRDALRKAYHHGGWGTEGGFEADEETSFRRTVEIDAAAIGELGLPEMPEAFYRRLAD
                     LFMVTSHWHVFPDAYPTLVGLKERSLIVGAVSNWVWNLPELLHALDLVAHFDFIAASA
                     RIGFEKPHPRIFAWALEQAKVEPAAVLHVGDHVDADVEGARAVGVGAVLIDRTGRYPA
                     EAVPDGVPVIRSLSELLPLVDARLDGHA"
     gene            complement(1817..2287)
                     /locus_tag="VFV59_03965"
     CDS             complement(1817..2287)
                     /locus_tag="VFV59_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU5325018.1"
                     /translation="MTAGPQFDPYAVLQVVPTCEQEVVHAAFKALAMRYHPDRDGSRR
                     AADRMAELNRAYALLRDPDGRRAFDRSRRMTVAGVSVAASASGAPTAPGTSRPATGGP
                     GASVLSFGRYSGWSLGDLARRDPDYLLWLSRHSSGIRYRTEIYSLLRTMGVSAA"
     gene            complement(2301..3404)
                     /locus_tag="VFV59_03970"
     CDS             complement(2301..3404)
                     /locus_tag="VFV59_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5325019.1"
                     /translation="MIDEPIAQPQPKSQPASPTPDWKLEVGEGTLLPRSALSVLTSLN
                     ERVAKGNIDDLVPIATGFVPMDKTIGGGLRPGELMLIGGAQGQGKTTMALQMARNMAA
                     SGQATVLYVCFEHDEEYLIQRMVAMESALSHLPQKSGAVKIQDVKKEVVSSYTASAET
                     GTKAKLGANPRLRPSLERIARYGSNLYLMRGTATQTTIENLRALVREHRGSDDERRLV
                     LFVDYLQKVPVIPEPNTEEEKVTRIVSGLKDIALAESVPVVAIVAADREGLKAKRLRN
                     HHLRGSSAINYESDIILILNDKYNIVAKVNIEFNPYQAQRYRDWVVATIEKNRGGQDN
                     VDLEYEKLFEYSCFDPTGRITAEKLIEERLYND"
     gene            3582..4406
                     /locus_tag="VFV59_03975"
     CDS             3582..4406
                     /locus_tag="VFV59_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU5325020.1"
                     /translation="MTDPDAFAPTASFMTANFVAREAGWSIPDWGAGDRATNEAFSPI
                     ETFRERFAALIDEAVNLGFDAIDLWEGHLNARWATPEHVSAAVSVLRDRGVTVTSLAG
                     WFGNDAERVAAVCRLAVAVSAPILGGGTKLLEDDRDALVRLLEEHDLRFGLENHPEKT
                     PGDVRARIGEDAGGRIGATVDTGWFGTQGYDAAAAILELGDRVLHVHLKDVRHAGVPH
                     ETCGYGEGVVPLERCVDALRDIGYRGGISVEHEPESYDPRDEIRAARKALAGWLGR"
     gene            4403..>5196
                     /locus_tag="VFV59_03980"
     CDS             4403..>5196
                     /locus_tag="VFV59_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5325021.1"
                     /translation="MTAGVGIVGCGDIAGRYAEDLARQPNVRLVAVTDLDAARAAAFG
                     EAHAARVHASLDELLADPEVEIVANLTSWSAHVPIGHRVVAAGRHLFSEKPLATTSED
                     ARDLLAAAAERGVLVGAAPIVHLGELAQTARRWLDEGRLGEVRMAYADVNWGWIEAWH
                     AQPRAFYEIGPLFDVGIYPVTLLTALFGPVAEVHADARRLKPTRMARDGSAFEIVAPD
                     WIVATLTFASGLVTRLTVNFYVADPARQRGIELHGDDGSLWLSNWF"
BASE COUNT          744 a         1830 c         1876 g          746 t
ORIGIN      
        1 gcggcctcgc gtgggtcgcg gacgcggtgg acgcgcggac cgcccaggcg gtgctcggac
       61 tcgatccgcc acgagctgag cgcgacgacc tggcgcccca tccgcagcgc aagtccgatc
      121 tcggacagcg tgccgtgccg accgcccacg gccaccacgg catcggcggc ggtgacgacg
      181 accaggttgc ggacgacgcc caggccggtg gcgatcggcg tgcggacgta ctcgttcgcc
      241 tccgaagcct ccgcgccggg gaggagaccg atggtctcgc cgcccgcacc gacagccccg
      301 cgggacgccg cccgcatgac gccgccgaga ccgccggtca ccaccaccgc ccctccccgc
      361 gccaggtgct ggcccacctc ctcggcccac tcgagcatgc ggtgcgcatc cgggccgcgc
      421 gggtcgccgt caccgatgac ggcgacgtag ggcgggcgtg gagtcgcggg acgatcggtc
      481 atgtggaact gcctccgagg agaacggcgg cgagtgactc gtagcgagcc ggcccgcggc
      541 cgaggtgcga acgcatcagg tgaatagcgg tcacgtcgag cacaccctcg ggcgcagccg
      601 ccgattcgag gaagggccga aggtcaaccg gatcgtggcg cgctcgggcc agggtcacgt
      661 gcgcccggaa ccggtcgtcg ggcggcaacc cgagctcccg gcgcacggcc gcggcgaggc
      721 gccgcaggtg gcgctccgga tcctccatgc cgtaccacag cacccgcgcc cgtccgctcg
      781 acgggaaggc gccaaggccc ccagtcgggt aggcggccga agccgcgcct ccggtcgccc
      841 ggttcagcgc cgcgagcaac gccgggactc gtccaggatc ggtgtcgccg aggaaggcca
      901 gggtcaggtg ccacccgtcg ggatcggtcc agcgcaggcc ggacgtgttg gggtgagcgc
      961 gccatcggtc agcagccgcc gccagcgacg cgcgcagctc ctcgccgagg gccacggcca
     1021 cgaagcagcg ccaactgctc gcgtcaggca tggccgtcga gccgcgcgtc gacgagcggc
     1081 agcagctcgc tcagggagcg gatgaccggc acgccgtccg gcacggcctc ggccgggtag
     1141 cggccggtcc ggtcgatcag cacggcgccg acgccgacgg cacgggcgcc ctcgacgtcc
     1201 gcgtccacat ggtcgccgac gtggagcacg gcggccggct cgaccttcgc ctgctccagc
     1261 gcccacgcga agatgcgcgg atgtggcttc tcgaagccga tgcgcgcgct ggcggcgatg
     1321 aagtcgaagt gcgcgaccag gtccagggcg tggagcagct ccggcaggtt ccagacccag
     1381 ttgctcacgg cacccacgat caggctgcgc tccttgagcc caacgagcgt cgggtaggca
     1441 tcggggaaga cgtgccagtg gctggtgacc atgaacaggt ccgccaggcg ccggtagaac
     1501 gcctcgggca tctcgggaag cccgagctct ccgatcgccg cggcgtcgat ctccaccgtg
     1561 cggcggaagc tcgtctcctc gtcagcctcg aagccgccct cggtccccca cccgccgtgg
     1621 tggtaggcct tgcgcagggc atcgcgcagg cggtcgatct cgacgtggat gccgaactcc
     1681 tcgaaggcga gcgcgtagac gtgctcccac gacggattgg ggcgcatcac ggtatcgccg
     1741 atgtcgagga agacagcgcg gagcggaggc agggcatcgg tcattggccg cccaagggta
     1801 gcgcgacgcc cgcgtgctag gcggcggaga cgcccatcgt gcggagcagg ctgtagatct
     1861 cggtgcggta gcggatgccg gacgagtggc gcgacagcca gagcaggtag tcggggtcac
     1921 gacgggccag gtcgcccagc gaccaacccg agtagcggcc gaaggacagc acgctggctc
     1981 ccggtccccc ggtggcgggc cgactcgtgc ccggcgccgt cggagcgccc gaggcgctcg
     2041 cggccaccga cacgccggcc accgtcatgc ggcggctgcg atcgaaggcg cggcggccgt
     2101 cgggatcgcg cagcagcgcg taggcacggt tgagctcagc catgcggtcg gcggcgcgac
     2161 gggagccgtc gcggtcgggg tggtaccgca tcgccagcgc cttgaacgcg gcgtgcacca
     2221 cctcctgctc gcaggtcggc acgacctgca ggacggcgta ggggtcgaac tgcggcccgg
     2281 ccgtcatcgg tgccggaccg tcagtcgttg tagagccgct cctcgatcag cttctccgcg
     2341 gtgatccgcc ccgtcggatc gaagcagctg tactcgaaga gcttctcgta ctcgaggtcg
     2401 acgttgtcct ggccgccgcg gttcttctcg atcgtcgcca ccacccagtc gcggtagcgc
     2461 tgcgcctggt acggattgaa ctcgatgttg accttggcga cgatgttgta cttgtcgttc
     2521 aggatcagga tgatgtcgga ctcgtagttg atggccgatg agccgcgcag gtggtggttc
     2581 cgcaggcgct tggccttcag cccctcgcgg tccgcggcca cgatcgcgac caccgggacc
     2641 gactcggcca gcgcgatgtc cttgagcccc gacacgatcc gggtgacctt ctcttcctcg
     2701 gtgttcggct ccgggatgac cggcaccttc tggaggtagt cgacgaacag caccagccga
     2761 cgctcgtcat cggacccgcg gtgctcgcgc accagcgccc gcaggttctc gatggtcgtc
     2821 tgcgtcgccg tgccgcgcat gaggtacagg ttcgagccgt agcgcgcgat gcgctcgagg
     2881 gacggccgca ggcgcgggtt cgcgcccagc ttggccttcg tgccggtctc ggcagaggcg
     2941 gtgtacgagc tcaccacctc cttcttgacg tcctggatct tcaccgcgcc cgacttctgc
     3001 ggcaggtggg agagcgccga ctccatcgcg accatgcgct ggatgaggta ctcctcgtcg
     3061 tgctcgaagc agacgtagag gacggtcgcc tggcctgatg cggccatgtt gcgcgccatc
     3121 tgcagcgcca tggtcgtctt gccctggccc tgcgccccgc cgatgagcat cagctcaccc
     3181 ggtcggagac caccgccgat ggtcttgtcc atcggcacga aaccggtggc aatcggcacg
     3241 aggtcgtcga tgttgccctt cgcgacacgc tcgttgaggc tggtcagcac gctcagcgcg
     3301 gagcgcggca ggagggttcc ctcccccacc tccagcttcc agtccggcgt cggcgacgcg
     3361 ggctgcgact tcggctgcgg ctgggcgatc ggctcgtcga tcatcgggtg gcaggctccc
     3421 ttggctcggg ctcgttcgtg cgttcgtgcg ctgcccaccg ggaggtgccc ggaagcaaca
     3481 cgcgagcgag tatacgttcc acctcctatg cctccaatgg cccgatcggc ctatgtgccg
     3541 atgcggcgcg cttcggcgcg ccggcgtgcc agagttgccc catgaccgat cccgatgcgt
     3601 tcgcgccgac cgcctccttc atgaccgcca acttcgttgc tcgcgaagcc gggtggagca
     3661 tccccgactg gggtgccggc gaccgcgcca cgaacgaggc cttctcgccg atcgagacgt
     3721 tccgcgagcg gttcgccgcc ctcatcgacg aggcggtgaa cctcggattc gacgcgatcg
     3781 acctgtggga ggggcatctc aacgcccggt gggccacgcc cgagcacgtc tcggccgccg
     3841 tgagcgtgct gcgcgaccgg ggcgtcacgg tcaccagcct ggccggctgg ttcgggaacg
     3901 atgccgagcg ggtcgcggcg gtgtgtcgcc tggcagtcgc cgtcagcgcg ccgatcctgg
     3961 gtggcggcac gaagctcctc gaggacgacc gggatgccct ggtgcgcctg ctggaggagc
     4021 acgacctgcg attcgggctg gagaaccacc cggagaagac gccgggcgat gtccgggcca
     4081 ggatcggcga ggacgccggc gggcgcatcg gggcgaccgt cgacaccggc tggttcggca
     4141 cgcaggggta cgacgccgcg gcggccatcc tggagctcgg agaccgggtg ctgcacgtgc
     4201 acctgaagga cgtgcggcac gccggcgtcc cccacgagac ctgtggctac ggcgagggtg
     4261 tcgtcccgct ggagcgttgc gtggacgcgc tgcgcgacat cggctatcgc ggtggcatca
     4321 gcgtcgagca cgagcccgag tcctacgacc cgcgcgacga gataagggcc gcccgcaagg
     4381 cgctggccgg gtggctgggc cgatgacggc cggcgtcggg atcgtcggct gcggcgacat
     4441 cgccggacgc tacgccgagg acctggcgcg ccagccgaac gtgcggctgg tcgcggtcac
     4501 cgacctcgac gccgcccggg ccgctgcctt cggcgaggcg cacgccgcgc gcgtccacgc
     4561 ctcgctggac gagctgctcg ccgaccccga ggtcgagatt gtcgcgaacc tgacctcgtg
     4621 gtcggcgcac gtgccgatag ggcatcgcgt cgtggcagcc ggccggcacc tgttcagcga
     4681 gaagccgctc gcgaccacca gcgaagatgc gcgcgacctc ctggcggccg ccgccgagcg
     4741 gggcgtcctc gtgggcgcgg ccccgatcgt ccacctcggc gagctggccc agaccgcgcg
     4801 caggtggctg gacgagggcc gcctgggcga ggtgcggatg gcgtacgccg acgtgaactg
     4861 gggctggatc gaggcgtggc atgcgcaacc gcgcgccttc tacgagatcg gaccgctgtt
     4921 cgacgtcggc atctaccccg tgacgctcct gaccgccctg ttcggcccgg tggcggaggt
     4981 ccacgccgat gcccgacgcc tgaagcccac ccggatggcc cgggacggca gcgcgttcga
     5041 gatcgtggcg ccggactgga tcgtggcgac gctgacgttc gcctccggcc tcgtgacgcg
     5101 gctcacggtg aacttctacg tcgcggatcc ggctcgccag cgcggcatcg agctgcacgg
     5161 cgacgacggc tcgctgtggc tctcgaactg gttcca
//
LOCUS       DASTYP010000084        16838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_297864, whole genome shotgun sequence.
ACCESSION   DASTYP010000084 DASTYP010000000
VERSION     DASTYP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16838
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297864"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFV59_03985"
     CDS             complement(<1..378)
                     /locus_tag="VFV59_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU5325022.1"
                     /translation="MTAMDLPADDAPAPRRRAPRVPLPASRRGVAWLVVLVIVGIFLA
                     VQFGRQVYANWEIGRRAEAIQAQIADVEAQNAQLQAELDYLRSDAYVSAEARRLANLG
                     APGEQVLIIPPGAEQPVPQQIAAA"
     gene            516..2798
                     /locus_tag="VFV59_03990"
     CDS             516..2798
                     /locus_tag="VFV59_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5325023.1"
                     /translation="MLTAPPSNADEAIAALLASPDLAPLVAAHRELPPRPPRHAPWPA
                     GIDPRLVDGLRGRGIEALYTHQAAAFEAASGGRNVVVVTPTASGKTLCYNLPVLDAVA
                     QDPAARALYLFPTKALAADQLVELRALAAAAELGLKTHTYDGDTPANIRSVVRAAGQV
                     VITNPDMLHAAILPHHTKWYKLFENLRYVVIDELHTYRGLFGSHVANVIRRLRRVCNH
                     YGADPTFICASATIANPRELAERVLEAQVELIDDNGAPAGRKHVLVINPPVANEELGI
                     RGSALLTGQRLAERLIRGGVQTIAFARSRTSVEVLTTYLRETFAPPPGHPHTIRGYRG
                     GYLPNERHEIEAGLREGRVRGVVATNALELGIDIGSLDAAISIGYPGTIASTWQQMGR
                     AGRRAGTSLSALVCSSAPIDQFLAAHPEYLFESSPEHGLINPDNLYVLMNHLRASSFE
                     LPVPATERFGIAETPMLLDVLQEDGWIRRAEDDRFYWSHENFPASDFSLRTGAPENVV
                     IVDTTGDRNRVLGEIDLFAAPLLVHPKAIYIHQGVQYHVDELDWEERKAFVTRTDVDY
                     YTDADLGVTLKVLEVFEEADGPPAGLRQRGEVMVAWKVTMFKKLKYHTHENVGWGSIS
                     LPETEMHTTACWLVPPAALVNRYDRDTLDGALIGLARVARTTAALLLMCDPRDLGVLA
                     QVQAPFTGRPTLYLYDAVPGGVGLSERLFSLTDDLVRACREAVDGCPCADGCPACVGP
                     AIEVGHRGKQVVSELLAGMS"
     gene            2810..4063
                     /locus_tag="VFV59_03995"
     CDS             2810..4063
                     /locus_tag="VFV59_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEU5325024.1"
                     /translation="MVVSQRSFADRLAGVRAAQNAVLRPCRPESHVDDRAGTLARWFD
                     ARTDVLEGGTALVVERSTTVPNAAAEALAALPPAAYFDTETTGLSTGAGTVIFLAAVA
                     RVSGERVTVRQFLLPDYPHERALLRALVRDLAAFDRLVTYNGRAFDVPMLAARLTVHG
                     LHREQVGIPAAHDDLLPHARRLFRRPLGGARLADVESGVLGVHRISDCAGSEVPARYF
                     GYLAGGSPDILAEVLDHNFQDVVSLALLEAQLCHLRAGGWREAPVLDPRGMALDLLRA
                     GAVDEALELVETAQASVTDPAEANALRRVASRLLIALGEVERAEELWTLGTRRASVDA
                     ATAWIEVARIRERHRGDLRGAMEAAAAASRVLDIAFALGRGGSMAEVGRTRLTVERRL
                     RRLRRWVEAAERRARREARPTRRTA"
     gene            complement(4060..4902)
                     /locus_tag="VFV59_04000"
     CDS             complement(4060..4902)
                     /locus_tag="VFV59_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5325025.1"
                     /translation="MPELLIVGGLTVDRFADGRRAAGGSVLHAAVAASGEGVRPTMAT
                     VAGEEPEARAGLDRLRAMGQVVVQASPRTTTYAHSEVDGRRVLVLEASSDPIQPAALP
                     NLPVVEVALFAPIADELPAAAIHDLRSRVHPGLTVLLIQGWLRRLVVGEPVHPLPLAA
                     IPDELWDAFALADAIVVSTEDITETPGDPFAQALDVRGRIGPRPILVLTLGTEGYLLD
                     DPQAPRIVASMPRTVVRDVPSVGAGDTFGAALALHLGAGRDAASAAEAATERVIAVLE
                     ARRG"
     gene            4920..5240
                     /locus_tag="VFV59_04005"
     CDS             4920..5240
                     /locus_tag="VFV59_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325026.1"
                     /translation="MRLFFYELHEGATDLLTDALLVSERDLTPAEFAGMVETARRTVL
                     ETYEEDTLVEAVARELERSHGFTYISDEKLRASMSVGVEEEDTFLVEQSDEFRSMIVE
                     LEKP"
     gene            complement(5237..6331)
                     /gene="ychF"
                     /locus_tag="VFV59_04010"
     CDS             complement(5237..6331)
                     /gene="ychF"
                     /locus_tag="VFV59_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5325027.1"
                     /translation="MQVTIVGLPGSGKTTVFNALTGGHAETGGFSGGRAAPNVSVVKV
                     PDERLDRLAALFNPKKTTHADVTYVDVAIPAGAAREGTINPDVLAQIRNADALLHVAR
                     AFDDPASAVSADPWRDVDDLDVEFTVADLSVIEKRLEKLRTQGRHGSQAERDAAQREQ
                     ELLERIEPHLSDGKPIRSFGLTDDEELLLRGYRFLTQKPVLVVLNVDEGRLPEAATLE
                     AAGRERYAQPHTDVAVLAGKIEAEIGELSDEDARLFMDDLGIAEPSRGRVIRLTYALL
                     GLFSFFTAGEDECRAWTLRTGSTAVDAAGAIHSDLARGFIRAQVIGYEDLLDAGSMAE
                     ARKRGTLRQEGKGYLVQDGDVIEVLFNVGR"
     gene            complement(6336..7364)
                     /locus_tag="VFV59_04015"
     CDS             complement(6336..7364)
                     /locus_tag="VFV59_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU5325028.1"
                     /translation="MDLPHRRSRVLSAAALTVLATPILGAPLSRELVGRIEEVDGVRC
                     ARISADGQIHGDADEGAFAAAEVLLLGSMPASVLDQVLARAPRLRWIHSASAGVDRIA
                     TRQVRERGLVVTNARGVFSRPIAEYVVMMSLAIAHRLPQLLELQRERTWQPLRGRELS
                     GLTVGIVGYGSIGVEVARLLEPFGCRVLATRRHPDRGSGGARNVELMGLDHLHDLLRA
                     SDIVVVAAPLTDETAGLIGAPELAEMREDAWLINVARGRLLDEVALRRALESGWIGGA
                     VLDVFTEEPLPSDSPLYRMPNLVITPHTSWSSDRVAERTVELFVENLRHDRAGEPLRN
                     VVDLAAGY"
     gene            complement(7331..8488)
                     /locus_tag="VFV59_04020"
     CDS             complement(7331..8488)
                     /locus_tag="VFV59_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU5325029.1"
                     /translation="MARNSSRLGAGIVVGGLLALGASVASREIMRRAGTRLIDWRAVR
                     RIAEQRLGEAGAPLDARLRAEAEAFYRSTLLRIEPVVAEEIGSPLPEALEVPAVVDRL
                     EWVDLNVATFRVLFDRIETTLLEQQSGPDTAGRAMSRLVNRTLSNQQLGFMLAFLARK
                     VLGQYDVSLLAAGPEVRGRLHFVEPNILGTAAALRLPRDEFRTFIALHEATHAFEFEA
                     HPWLRPYFAGLVGETIELLAAESGGLMARVREAVAGGSGHWLERIMTPGQRDLFNRTQ
                     ALMSLLEGYSNHVMNAAGERILPNFHQIHDQFERRGERRGALERAIMRITGLDLKMEQ
                     YAAGERFADAVITARGREFLNRVWSGPEVLPTLEEIRTPDLWISRIEGAGS"
     gene            complement(8493..9335)
                     /gene="murI"
                     /locus_tag="VFV59_04025"
     CDS             complement(8493..9335)
                     /gene="murI"
                     /locus_tag="VFV59_04025"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5325030.1"
                     /translation="MEQHHDPRPIGVFDSGVGGLTVLAELHRRLPDEPTIYLGDNART
                     PYGPRPADEVRRFTLAAVEWLLAQDVKLLLLACNTATARALPQVRERADVPVLGVIRP
                     GAVAAAAATRSRHVGVIATAGTVESGAYVDAIVEADAHTVVAQLACPELVPMVEAGIL
                     DGRRAETVLRGYLDPMLAAEPEMDTLLLGCTHYPLLRDPIERIVGAGISVVDSAFTTA
                     LAVEDLLDALGRRAPGGGTPRHRVATTGDVDAFVTVAERLFGDRLPDVESVEIESQAP
                     LPVD"
     gene            complement(9366..10514)
                     /locus_tag="VFV59_04030"
     CDS             complement(9366..10514)
                     /locus_tag="VFV59_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5325031.1"
                     /translation="MAGEQIRLLVVEDVPQVASHIRALLNAQSQIKMLDVVTAGDRAV
                     PSVSDMKPDVVIVDALLQGRVSGQQVAEQIRRAEPQVGVIMLTVPQNPVREDAERGVD
                     AVLTMPFTGFDLTTMVRKVGETRAVESARSGSLVVSLFSPKGGVGRTTLAYNLAVALG
                     EGHRVCLVDGSLQFSDLRGLLRVPAVAPSIVNLPTDRIKESDLGEVAWRDPSGIDILL
                     APPRVEMSEMITTRDIEKVLSLLRQLYEFVIIDTRAGLSDDVLVFLDSSDLILQVLSY
                     DAMAIRSLAMAGEAFNAIGYPPSKLATVLNRSDAMGGFDRSDVEQALGFKIDYEVPSD
                     GRLVLNANNEGIAFVSSSPEAPIAQAVKRVADSLATHLRERSPALARR"
     gene            complement(10519..11391)
                     /locus_tag="VFV59_04035"
     CDS             complement(10519..11391)
                     /locus_tag="VFV59_04035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5325032.1"
                     /translation="MGRAARAGRRTPSGTGAGGLSDILAGQPSDEALLAELYDLEHDE
                     IADDLVFYREHARRHRGAVADLGCGSGRLFRPFADGGASRILGIDGSPALLHRAEARI
                     GSDGMLQRMRDEGRIELAEGDVRTVRRRDRFRLVVLAGVIAHLDGPEDCLRTLSAAAR
                     LLDRDGWLIVDTIGPGGLPPRDLPLSVDWERRMGDRRVIRRSALVRRETPDGLHVAYS
                     TLTDVIEPDGTMGRLPAGFRLWYPSPTALIGLAEEAGLAVEAGYGSHDLDPLDNESER
                     CIVVLRRTADPGSG"
     gene            complement(11331..12530)
                     /locus_tag="VFV59_04040"
     CDS             complement(11331..12530)
                     /locus_tag="VFV59_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5325033.1"
                     /translation="MVEATLLEAVDAVLPDALGLSHRVHATPEIAFQERQASAWTAEL
                     LRSHGFDVTAPAGGLETAFVARWAGDRPGPVIAFAGEYDALPEVGHGCGHNLMCSSSA
                     GAAVAAARVLGRDFAGEIRFIGTPAEEAGNGKVHLIQAGLFADVDVCLQIHPSDSNSA
                     EVLALAVTEVGVTFHGKLAHASADPWLGRNALDAIVLLYTMVAQWRQHLRPGERVHGI
                     ITHGGAAPNIVPDLTSGRWYLRTPVDGNLDAMIERFSAMADAAAAGTGCTVDLEIDPA
                     NRCRTMLNNPTLLSIWRRHLADAGIADDPVDPNPGSTDMGNVSHEVPTIHPYLAIGPR
                     GTPGHSHEFADHAGGEDGDRMLPNAIRILAATAIDLVRDPALVERAWDELRAQGGGRR
                     RGQEPAG"
     gene            complement(12548..12949)
                     /locus_tag="VFV59_04045"
     CDS             complement(12548..12949)
                     /locus_tag="VFV59_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325034.1"
                     /translation="MTGAHRLQVASLILLGVFGLVVVVAMASVLCPADVPGQSCPDAT
                     RNVALVVAAAAASVAVLVTPFAFLGEVLGRRRIVYRGAWWRAGRRGALVGLVIAAFAG
                     LRLGGALSVPVAIFIVILAAAVEWLLARRDP"
     gene            complement(12946..15972)
                     /locus_tag="VFV59_04050"
     CDS             complement(12946..15972)
                     /locus_tag="VFV59_04050"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEU5325035.1"
                     /translation="MPPVVPSPDQLPIFEELVPATRPRRPHLRIQPPLLADLTARQRK
                     AVTHGEGPLLIVAGAGTGKTTVITRRIAWLIAEKRALPSEILALTFTDRAALEMTERV
                     DRLVPYGHNDAVISTFHAFGDRLLREHALEAGLSDRSTVLSRAEQVIFLREHLFELPL
                     DRYRPLGDPTRFLSAMVTLISRLRDEDVPPAAYLEVAERMRAESAAHREDEALAERAA
                     THAELAATYAAYEELMRANDRLDFGDQVSLALRLLREHPAVLEEERRRYRYILVDEFQ
                     DTNHAQWELVKLLAAEHGNVTVVGDDDQSIYRFRGAALGNILGFRATYPKATSVVLVD
                     NFRSRQGILDAAHRLIRHNDPDRLEAREGIDKRLRARSRFATPEPETGPIGLTGYTTV
                     SDEADAVADRIAASLRAGRRAGDHAVLVRGNRDADPYLRALNMAHIPWRFSGTAGLYR
                     QAEVRVLISFLRAVNDPDDSVSLYDLATSEIFGLAPADVTVALSRARRRRSSLADALA
                     EAARDPDGSPFGTRALEVVGRLLGTLESHRAMSTERSCGELLYHFITTSGWLARLAHE
                     ARETGEERLANVARFFDIVRRQGALLRDDRLPFLVAQLDTLIETGDDPSTADADTDDG
                     GAVHVLTYHKAKGLEFPVVFLVGVTADRFPGRERGDALEMPDELVRDAVDPGAGQHAA
                     EERRLFYVGMTRAKEQLHLSWAEDEGWRRSRAASQFVTEALDLPPATPRETVQPALLE
                     RLARHSGSPTSTAPAPARPALGDRPLMLSYGQVNDYLDCPARYRYAHVVRLPTPVSHQ
                     MVYGRALHAAAQAFHRRQLAGRPMEEAGLHAALDSNWESVGFLTREHEEARKAAAHEA
                     IGRFWTEQQRDPARPTAVEQDFTVSFGPDRIRGRYDRIDRDEAGRVVITDYKSSDVRD
                     LVTANRRSRESLQLSIYALAYEAQHGQLPDELALHFLESGLVGRSEPTEKRLERATEK
                     LGSAAAGIRAGNFEANPSVMRCGYCPFREICPDASR"
     gene            complement(16021..16674)
                     /locus_tag="VFV59_04055"
     CDS             complement(16021..16674)
                     /locus_tag="VFV59_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325036.1"
                     /translation="MTGGPIEPEQRRVAERLILAALRRFHREQPLSADIRTDALVARV
                     RDAARRRPPPRHRGATPLELDDVGLRDVIEAMVEDGRLRRTGRRVRLPDREPGLAPEM
                     AERVERLLSGLREARASPPRVEGPAARLGIPPAVLDQLRSGGLLRHVADGIDYPSDVW
                     ARLAARVEEMRGTVSVARLRDELGTSRRHAEAILATIGNRPRRPTGPPRSRDRRPVR"
BASE COUNT         2363 a         6188 c         5986 g         2301 t
ORIGIN      
        1 cgccgcggcg atctgctgcg gcaccggctg ctcggcgccg ggcgggatga tcaggacctg
       61 ctccccggga gctccgaggt tcgcgaggcg acgggcctca gcgctgacgt aggcgtcgct
      121 gcgcaggtag tcgagctcgg cctggagctg cgcgttctgc gcctcgacgt cggcgatctg
      181 cgcctggatc gcctccgcac gacggccgat ctcccagttc gcgtacacct gccggccgaa
      241 ctggacggcc aggaagatcc cgacgatcac gagcaccacc agccatgcca ctccgcgacg
      301 ggaggcgggc agcggcacgc gaggcgcgcg ccggcgcggt gcgggcgcgt cgtcggcggg
      361 cagatccatg gcggtcatcg gcgccaagtc tagcccaccg gacaacctcg accgccacgt
      421 cgcgacagca gagctgtcgc atcggccctc gaggattcga acacctgtgc taggttgcac
      481 cgaacgtccg ttctaattcg cgcctggagg agccgatgct gaccgcaccc ccgagcaacg
      541 ccgacgaggc gatcgccgcc ctcctggcca gccccgacct cgcgccgctg gtcgcagccc
      601 accgggagct gccgcctcga ccgccacgcc acgcgccgtg gccggccggt atcgatcccc
      661 ggctcgtgga cgggttgcgc gggcgcggga tcgaggcgct ctacacccac caggccgccg
      721 ccttcgaggc ggccagcggg ggccgaaacg tggtcgtggt cacgccgacc gcctcgggca
      781 agacgctctg ctacaacctc cccgtcctcg acgccgtggc gcaggacccc gcggcgcggg
      841 cgctctacct cttccccacc aaggcgctgg ctgccgacca gctggtggag ctgcgggcac
      901 tggcggcggc cgcggagctc gggctcaaga cccacaccta cgacggcgac acgccggcga
      961 acatccgcag cgtggtgcgc gccgcggggc aagtggtcat caccaacccg gacatgctcc
     1021 acgcggcgat ccttccccac cacaccaagt ggtacaagct gttcgagaac ctgcgctacg
     1081 tcgtcatcga cgagctccac acctaccgcg ggctcttcgg cagccacgtc gcgaacgtca
     1141 tccgccgcct gcggcgggtc tgcaaccact acggcgccga tccgaccttc atctgcgcca
     1201 gcgccaccat tgccaacccg cgcgagctcg ccgagcgcgt gctggaggcg caggtcgagc
     1261 tgatcgacga caacggcgcc ccggccgggc gcaagcacgt cctcgtcatc aaccccccgg
     1321 tggcgaatga ggagctcggc atccgcggct cggcgctgct cacgggccag cgcctggccg
     1381 agcggctcat ccgcggcggc gtacagacca tcgccttcgc ccgctcgcgc acctccgtcg
     1441 aggtcctgac cacctacctg cgcgagacgt tcgctccccc gcccggccac ccgcacacca
     1501 tccggggcta tcggggcggg tacctcccga acgagcgcca cgagatcgag gccggcctgc
     1561 gcgagggtcg cgtccgtggc gtggtggcca ccaacgcgct cgagctgggc atcgacatcg
     1621 gctcgctgga cgccgccatc agcatcggct accccggcac catcgccagc acctggcagc
     1681 agatgggacg tgccggacgg agggcgggca ccagcctctc cgccctggtg tgctcatcgg
     1741 cgcccatcga ccagttcctg gccgcgcacc ccgagtacct gttcgagagc tcgcccgagc
     1801 acggcctcat caaccccgac aacctctacg tcctgatgaa ccacctgcgg gcgtcgagct
     1861 tcgagctgcc ggtgcctgcc acggaacgct tcgggatcgc cgagacgccg atgctcctgg
     1921 acgtgctgca ggaggacggc tggatccggc gcgcggagga cgaccggttc tactggagcc
     1981 acgagaactt cccggccagc gacttcagcc tgcgcaccgg cgcaccggag aacgtcgtca
     2041 tcgtcgacac cactggcgac cggaaccgcg tgctgggcga gatcgacctg ttcgccgccc
     2101 cgctcctggt ccaccccaag gccatctaca tccaccaggg cgtgcagtac cacgtcgacg
     2161 agctcgactg ggaggagcgc aaggccttcg tcacccgcac ggacgtcgac tactacaccg
     2221 atgcggacct cggcgtcacc ctgaaggtgc tcgaggtctt cgaggaggcc gatgggccgc
     2281 cggccgggct gcgccagcgc ggcgaggtga tggtcgcctg gaaggtgacg atgttcaaga
     2341 agctcaagta ccacacccac gagaacgtcg gctggggctc gatcagcctg ccggagaccg
     2401 agatgcacac gaccgcctgc tggctcgtcc cgccggcggc cctcgtgaac cggtacgacc
     2461 gggacacgct cgacggggcg ctcatcggcc tggcgcgggt ggcgcgaacc acggccgccc
     2521 tgctgctgat gtgcgaccca cgggacctcg gggtcctggc ccaggtccag gcacccttca
     2581 ccggccggcc cacgctctac ctgtacgacg cggtccccgg cggcgtcggg ctttccgagc
     2641 ggctcttctc cctcaccgat gacctggtgc gggcctgccg cgaggccgtc gacggatgtc
     2701 cgtgcgccga tggctgtccg gcctgcgtcg gtccggccat cgaggtcggc catcgcggca
     2761 agcaggtcgt gtccgagctg ctcgccggga tgagctgagg ccgagttcca tggtcgtctc
     2821 gcagcgctcc ttcgccgatc gcctcgccgg cgtccgggcg gcgcagaacg ccgtgctgcg
     2881 cccgtgccgt cccgagtccc acgtcgacga ccgcgccgga accctggcgc gctggttcga
     2941 cgcccgcacc gacgtcctgg agggaggaac ggcgctcgtc gtggagcgaa gcaccacggt
     3001 gcccaacgcc gccgccgaag cgctcgccgc gctgccgccg gcggcctact tcgacaccga
     3061 gacgacgggc ctctccaccg gcgcggggac cgtgatcttc ctcgccgccg tggcgcgggt
     3121 gagcggtgag cgcgtcacgg tcaggcagtt cctgctcccc gactacccgc acgagcgggc
     3181 gctgctccgc gcgctcgtcc gcgacctcgc cgccttcgac cggctggtca cgtacaacgg
     3241 ccgcgcgttc gatgtcccga tgctggcggc gcgcctgacc gtccacggac tccaccgcga
     3301 gcaggtgggc atccccgccg ctcacgacga cctgctgccg cacgcccgtc gcctcttccg
     3361 ccgtccgctg ggtggtgcac gcctggccga cgtcgagtcc ggcgtcctgg gcgttcaccg
     3421 catctcggac tgcgccggca gcgaggtccc ggcgcgctac ttcggctacc tggccggcgg
     3481 gtcaccggac atcctggccg aggtgctcga ccacaacttc caggacgtcg tcagcctggc
     3541 gctgctggag gcgcagctgt gccacctgcg cgccgggggc tggcgagagg ccccggtgct
     3601 cgatccccgc ggtatggcgc tcgacctgct gcgtgccggc gccgtcgacg aggcgctcga
     3661 gctggtcgag accgcccagg cgagcgtcac ggatccggcc gaggcgaacg cgctgcgccg
     3721 ggtcgcctct cgcctgctca tcgcgctcgg cgaggtggag cgtgccgagg agctctggac
     3781 gctcgggacg cggcgcgcct ccgtggacgc ggcgaccgcc tggatcgagg tcgcccgaat
     3841 ccgcgagcgg caccgtggcg atctgcgcgg ggccatggag gccgccgccg ccgcgtcacg
     3901 cgtcctcgac atcgccttcg ccctggggcg cggcggctcg atggcggagg tgggccggac
     3961 ccgcctcacc gtcgaacgga ggctccggcg cctccggcgc tgggtcgagg cagcggagcg
     4021 gcgcgcacgc cgcgaggcac ggccgacgcg acgaaccgcc tagcctcgtc gggcctccag
     4081 caccgcgatc acccgctcgg tggcagcctc ggccgccgac gccgcatcgc ggccggcgcc
     4141 caggtgcagc gcgagcgcgg caccgaacgt gtcgcccgcc ccaaccgacg gcacgtcccg
     4201 gacgacggtg cgcggcatgc tggccacgat gcgaggggcc tgcggatcgt cgagcaggta
     4261 gccctccgtc ccgagcgtga gcaccaggat cggtcgcggc ccgatccgtc cgcggacgtc
     4321 gagggcctgg gcgaagggat cacccggcgt ctcggtgatg tcctccgtcg acaccacgat
     4381 ggcatcggcc agggcgaacg catcccacag ctcgtccggg atggccgcga gcggcagcgg
     4441 gtggaccggc tccccgacga ccagccggcg cagccagccc tggatcagga gcacggtgag
     4501 gccggggtgg acgcgcgatc gcaggtcgtg aatggcggcg gccggcagct cgtccgcgat
     4561 gggggcgaag agggcgacct ccaccaccgg caggttcggc aacgctgcgg gctggatcgg
     4621 atcgctggat gcctcgagga ccagcacgcg ccgaccgtcg acttccgagt gcgcgtaggt
     4681 ggtcgtgcgc ggcgatgcct gcacgacgac ctggcccatc gcgcgcaggc gatcgagacc
     4741 cgcacgcgct tcaggctcct cacccgcgac ggtcgccatc gtcgggcgca ccccctcgcc
     4801 ggacgccgcc accgcggcgt gcagcaccga cccacccgcc gcccgccggc cgtcggcgaa
     4861 gcggtcgacg gtcaggccgc cgacgatcag cagctcgggc atcggtctat catcggtgga
     4921 tgcgactctt cttctacgag ctccacgagg gcgccaccga cctgttgacc gatgcgctcc
     4981 tcgtctccga gcgcgacctt actccagccg agttcgccgg gatggtggag acggcgcgcc
     5041 ggacggtgct cgagacctac gaggaggaca cgctggtcga agccgtcgcc cgcgagctgg
     5101 agcgctcgca cggcttcacc tacatcagcg acgagaagct tcgcgcgtcg atgagcgtgg
     5161 gcgtcgagga agaggacacg ttcctggtcg agcagagcga cgagttccgc tcgatgatcg
     5221 tggagctcga gaagccctag cggccgacgt tgaacagcac ctcgatgacg tcgccgtcct
     5281 gcaccaggta gcccttcccc tcctgacgca gcgtgccgcg cttgcgcgcc tcggccatcg
     5341 atccggcgtc cagcaggtcc tcgtagccga tgacctgggc gcggatgaag ccgcgcgcca
     5401 ggtccgagtg gatggcgccg gccgcatcca cggccgtcga gcccgtgcgc agcgtccacg
     5461 cccggcactc gtcctcgccc gcggtgaaga agctgaagag gcccagcagc gcgtaggtca
     5521 gacggatcac ccggccgcgt gacggctcgg cgatcccaag gtcatccatg aagaggcgcg
     5581 cgtcctcatc gctcagctcg ccgatctccg cctcgatctt gccggccagc accgccacgt
     5641 cggtgtgcgg ctgcgcgtag cgctcgcggc cggccgcctc gagcgtcgcc gcctccggca
     5701 gcctcccctc gtccacgttc agcaccacga gcacgggctt ctgggtcagg aagcggtatc
     5761 cccgcagcag cagctcctcg tcgtcggtga ggccgaacga gcggatcggc ttcccatcgg
     5821 acaggtgcgg ctcgatgcgc tccagcagct cctgctcgcg ctgggccgca tcgcgctccg
     5881 cctgcgagcc gtggcgaccc tgcgtgcgca gcttctccag ccgcttctcg atgaccgaca
     5941 gatcggccac cgtgaactcc acgtccaggt cgtcgacgtc gcgccacggg tcggcggaca
     6001 ccgccgaggc cggatcgtcg aaggcgcggg cgacgtgcag cagcgcatcg gcgttgcgga
     6061 tctgggccag gacgtccggg ttgatggttc cctcccgggc ggcgccggcg gggatggcga
     6121 catcgacgta ggtcacgtcg gcgtgggtcg tcttcttggg gttgaacagc gccgcaagcc
     6181 ggtccaggcg ctcgtccggc accttgacca cgctcacgtt cggcgccgcg cgcccaccgg
     6241 agaagccacc cgtctcggca tggccgccgg tcagcgcgtt gaagacggtc gtcttcccgc
     6301 tgccgggaag gccgacgatg gtgacctgca tcggctcagt agcctgctgc caggtccacg
     6361 acgttgcgca gcggctcgcc ggcccggtcg tggcgcaggt tctcgacgaa cagctcgacc
     6421 gtgcgctccg ccacgcggtc ggacgaccac gaggtgtgcg gcgtgatcac caggttcggc
     6481 atgcggtaga ggggcgagtc ggagggcagt ggctcctcgg tgaagacgtc gagtaccgcg
     6541 ccgccgatcc agccggactc gagcgcccgt cgcagcgcca cctcgtccag gagacgaccg
     6601 cgggcgacgt tgatcagcca ggcgtcctcg cgcatctccg ccagctcggg cgcaccgatc
     6661 aggccggccg tctcgtcggt cagcggcgcc gccacgacca cgatgtccga cgcgcggagc
     6721 aggtcgtgca ggtggtccag ccccatcagc tcgacgttgc gcgcgccccc ggacccccgg
     6781 tcgggatgcc gccgcgtggc gagcacccgg cagccgaacg gctccagcag ccgcgccacc
     6841 tcgacgccga tgctgccgta cccgacgatc ccgaccgtga ggccggacag ctcccgaccg
     6901 cgcagcggct gccaggtccg ctcgcgctgc agttccagga gctgcgggag ccgatgcgcg
     6961 atggccagcg acatcatcac cacgtactcg gcgatgggtc ggctgaagac gccgcgcgcg
     7021 ttggtgacca ccagtccgcg ctctcggacc tgccgtgtcg cgatccggtc gacgcccgcg
     7081 gacgcggagt gtatccagcg caggcgtggg gcgcgggcga ggacctggtc gagcacggac
     7141 gccggcatcg agccgagcag gagcacctcc gcagcggcga aggcgccttc gtcggcgtcg
     7201 ccgtggatct gtccatcggc gctgatccgc gcgcaacgga cgccgtcgac ctcctcgatc
     7261 cgaccgacca gctcgcgcga cagcggcgcc cccaggatcg gcgtcgcgag gacggtcagc
     7321 gccgccgcgc tcaggacccg gctccttcga tgcgggagat ccacaggtcg ggggtgcgga
     7381 tctcctcgag cgtcggcagc acctcgggtc cgctccagac gcggttgagg aactcgcggc
     7441 cacgggccgt gatgaccgca tcggcgaagc gctcgccggc ggcgtactgc tccatcttca
     7501 ggtccaggcc cgtgatgcgc atgatcgccc gctccagcgc cccgcggcgc tcgccgcgtc
     7561 gctcgaactg gtcgtggatc tggtggaagt tgggcaggat gcgctcgccg gccgcgttca
     7621 tgacgtggtt cgagtagccc tcgagcagcg acatcagggc ctgcgtccgg ttgaagaggt
     7681 cgcgctgccc cggcgtcatg atgcgctcca gccagtggcc gctgccgccg gcgaccgcct
     7741 cgcgcacccg ggccatgagg ccgcccgact ccgccgcgag cagctcgatc gtctccccga
     7801 cgagcccggc gaagtacggc cgcagccacg gatgcgcctc gaattcgaag gcgtgcgtcg
     7861 cctcgtgcag ggcgatgaag gtgcggaact cgtcgcgggg cagccgcagg gcggcggccg
     7921 tccccaggat gttgggctcc acgaagtgca ggcggccgcg cacctcgggg ccggcggcca
     7981 gcaggctgac gtcgtactgg ccgagcacct tgcgcgccag gaaggcgagc atgaagccga
     8041 gctgctggtt gctcagggtc cggttgacca ggcgcgacat ggcgcggccc gcggtgtccg
     8101 ggccggactg ctgctccagc agcgtcgttt cgatgcggtc gaacagcacg cggaacgtcg
     8161 cgacgttgag gtcgacccac tcgagtcggt cgaccaccgc gggaacctcg agcgcctcgg
     8221 gcagcggcga gccgatctcc tcggccacga cgggctcgat ccgcagcagg gtcgaccggt
     8281 agaacgcctc cgcctcggct cgcagccgcg cgtcgagcgg cgcgccggcc tctccgagtc
     8341 gctgctcggc gatgcgacgc acggcacgcc agtcgatgag gcgcgtgccg gcgcgacgca
     8401 tgatctcgcg cgacgcgacg ctcgcaccaa gggcgaggag gccgccgacg acgatgccgg
     8461 cgccgaggcg gctgctgttt cgggccatgc cctcagtcta cgggaagcgg cgcctgggac
     8521 tcgatctcga cgctttccac gtccggcagc cggtccccga acagccgctc ggcgaccgtg
     8581 acgaaggcat ccacgtcgcc ggtcgtcgcg acgcggtggc gcggggtccc tccaccgggt
     8641 gcgcgacgtc ccagggcgtc gagcagatcc tcgacggcca gcgccgtggt gaacgcggaa
     8701 tcgaccaccg agatccccgc tcccacgatc cgctcgatcg ggtcgcgcag gagcgggtag
     8761 tgggtgcagc cgaggaggag cgtgtccatc tcgggctccg ccgccagcat cgggtcgagg
     8821 tagccgcgca gcacggtctc ggcgcgacgc ccgtccagga tcccggcctc caccatgggt
     8881 accagctcag ggcaggcgag ctgggcgacg acggtgtgcg catcggcctc gacgatggcg
     8941 tcgacgtacg cccccgactc caccgtgccg gcggtggcga tgacgccgac atgccgggac
     9001 cgggtcgccg cggccgccgc gaccgcgccg gggcggatga cgccgaggac cggcacgtcc
     9061 gctcgctcac ggacctgcgg cagcgcgcgg gccgtcgccg tgttgcaggc cagcaggagg
     9121 agcttgacgt cctgcgccag cagccactca acggccgcca gcgtgaagcg ccgcacctcg
     9181 tcggccggac gcggcccgta cggggtgcgg gcgttgtcgc cgaggtagat cgtcggctcg
     9241 tccggcaggc ggcgatgcag ctcggcgagg accgtcagcc cgcccacgcc ggagtcgaag
     9301 acgccgatcg gacgcggatc gtggtgctgc tccacgcgat gctcgagtgg ctcggggagg
     9361 cggcgctagc gccgggccag cgccggcgag cgctcgcgca ggtgggtcgc cagcgaatcg
     9421 gcgacgcgct tcacggcctg cgcgatcggc gcctccgggc tcgagctgac gaaggcgatc
     9481 ccctcgttgt tcgcgttcag caccaggcgg ccgtcggacg gcacctcgta gtcgatcttg
     9541 aagcccagcg cctgctccac gtcggatcgg tcgaagccgc ccatcgcatc ggatcggttc
     9601 agcacggtcg ccagcttcga gggtgggtag ccgatggcgt tgaaggcctc cccggccatc
     9661 gccaggctgc ggatggccat ggcgtcgtag ctgagcacct gcaggatgag gtcggaggaa
     9721 tccaggaaga ccagcacgtc gtcggacagg cccgcgcgcg tgtcgatgat cacgaactcg
     9781 tacagctgcc gcagcagcga gagcaccttc tcgatgtcgc gggtcgtgat catctccgac
     9841 atctcgaccc gcggcggcgc caggaggatg tcgatgcccg acgggtcgcg ccacgcgacc
     9901 tcgcccaggt ccgactcctt gatgcggtcg gtcggcaggt tgacgatgct gggcgccacg
     9961 gccggcacgc ggagcagccc gcggaggtcg ctgaactgga gcgagccgtc caccaggcag
    10021 acccgatggc cctcgccgag ggccacggcc aggttgtagg ccagcgtcgt gcgcccgacg
    10081 ccgcctttcg ggctgaagag gctcaccacc agcgagccgg agcgcgcgga ctcgaccgcg
    10141 cgcgtctccc ccaccttgcg aaccatcgtg gtcaggtcga agccggtgaa cggcatggtc
    10201 aggacggcgt cgaccccgcg ctcggcatcc tcgcgcaccg ggttctgtgg gacggtcagc
    10261 atgattacgc cgacctgcgg ctcggcccga cggatctgct cggcgacctg ctgcccgctg
    10321 acgcgtccct ggagcagcgc gtcgacgatc accacgtcgg gcttcatgtc cgacacgctc
    10381 gggacggcgc ggtctccggc ggtcacgacg tcgagcatct tgatctggga ctgggcgttg
    10441 agcagcgctc ggatgtgcga cgccacctgc gggacatcct caaccaccag gagccgaatc
    10501 tgttcgcctg ccatcccctc acccactgcc cggatcggcc gtgcggcgca gcacgacgat
    10561 gcagcgctcg ctctcgttgt cgagcggatc caggtcgtga gacccgtacc cggcctcgac
    10621 ggccagcccc gcctcctcgg ccaggccgat gagcgcggta ggtgatggat accagaggcg
    10681 gaagccagcc ggaagcctgc ccatcgtacc atcgggctcg atcacgtccg tgagcgtgct
    10741 gtacgcgacg tggagcccgt ccggcgtctc gcgacgcacg agggcgctgc gccggatcac
    10801 gcgccgatcc cccatgcggc gctcccagtc gacgctcagc ggcaggtcgc gtgggggcag
    10861 gccgccggga ccgatggtgt ccacgatcag ccagccgtcc cggtccagca gccgcgctgc
    10921 cgccgagaga gtgcgaaggc agtcctcggg accgtcgagg tgcgcaatga cgccggccag
    10981 caccaccaac cggaaccgat cccggcgccg caccgttcgc acgtcgccct cggccagctc
    11041 gatgcgcccc tcgtcccgca ttcgctggag catgccgtcg gagccgatgc gcgcctccgc
    11101 gcggtggagg agggccggtg acccgtcgat gcccaggatg cggctggcac cgccgtcggc
    11161 gaagggccgg aacagccgtc ccgagccgca gcccaggtcc gcgaccgcgc ctcgatgccg
    11221 gcgcgcgtgc tcgcggtaga agacgaggtc gtcggcgatc tcatcgtgct cgaggtcgta
    11281 tagctcggcc agcagcgcct cgtcggacgg ctgtccggcc aggatgtcgc tcagcccgcc
    11341 ggctcctgtc cccgacggcg tccgccgccc tgcgcgcgca gctcgtccca ggcacgctcg
    11401 accagcgctg ggtcgcggac caggtcgatc gccgtggccg cgaggatgcg gatcgcgttc
    11461 ggcagcatgc ggtcgccgtc ctccccgccg gcgtggtcgg cgaactcgtg cgagtggccg
    11521 ggcgtgcccc gaggcccgat ggccaggtag gggtggatcg tcggcacctc gtggctgacg
    11581 ttgcccatgt ccgtgctgcc ggggttcggg tccaccggat cgtcggcgat gcccgcatcg
    11641 gccagatggc gccgccagat cgacagcagc gtggggttgt tgagcattgt gcggcatcgg
    11701 ttggccgggt cgatctcgag gtcgaccgtg cagcccgtcc ccgccgcggc ggcatcggcc
    11761 atggcgctga agcgctcgat catggcgtcg agattcccgt ccacgggcgt gcgcaggtac
    11821 cagcggccgc tcgtcaggtc cgggacgatg ttcggcgccg caccgccgtg cgtgatgatg
    11881 ccgtgcaccc gctcgccggg gcggaggtgc tgccgccact gcgcgaccat cgtgtacagc
    11941 aggacgatcg cgtcgagcgc gttgcggccc agccacgggt cggcgctggc gtgcgccagc
    12001 ttgccgtgga acgtgacgcc gacctcggtc accgcgagcg ccaggacctc ggcgctgttc
    12061 gagtccgagg gatggatctg caggcagacg tcgacgtcgg cgaagaggcc cgcctggatc
    12121 aggtgcacct tcccgttgcc ggcctcctcc gcgggcgtgc cgatgaagcg aatctccccg
    12181 gcgaagtcgc gtcccagcac ccgcgcggcg gcgaccgcgg cgccggcact cgaggagcac
    12241 atcaggttgt ggccgcagcc gtgccccacc tccggaagcg cgtcgtactc accggcaaag
    12301 gcgatcaccg gccctggccg gtcgccggcc cagcgcgcga cgaaggccgt ctccaggccg
    12361 ccggccgggg cggtcacgtc gaagccgtgg gagcggagca gctcggccgt ccacgccgag
    12421 gcctggcgct cctggaacgc gatctcgggg gtggcgtgga cgcggtgcga caggcccagc
    12481 gcgtcgggca ggacggcgtc gaccgcctcg aggagggtcg cctcgaccat cgggtacctc
    12541 cgtcggctca gggatcgcgc cgggcgagga gccactcgac cgcggcggcg aggatgacga
    12601 tgaagatggc gaccggcacg ctcagcgcgc cgcccagccg caggccggcg aaggccgcga
    12661 tgacgagccc caccagcgcc ccgcgacggc cggcccgcca ccacgcgcca cgatagacga
    12721 tgcgtcggcg gccgagcacc tcgcccagga acgcgaaggg ggtgaccagg acggcgacgc
    12781 tcgccgcggc cgcggcgacg acgagggcga cgttgcgggt ggcgtccgga cacgactggc
    12841 ccgggacgtc ggcggggcac agcacgctgg ccatggcgac gacgacgacc aggccaaaga
    12901 cgccgagcag gatcagcgac gcgacctgca ggcggtgggc tcccgtcatc gcgacgcgtc
    12961 ggggcaaatc tcccggaacg ggcagtatcc acagcgcatg accgatgggt tcgcctcgaa
    13021 atttcccgcg cggatgccgg ccgccgcgct gcccagcttc tccgttgccc gctcgagacg
    13081 cttctccgtc ggctccgagc ggccgaccag gccggactcc aggaagtgga gcgccagctc
    13141 gtccggcagt tgtccgtgct gggcctcgta ggcaagggcg tagatcgaca gctggagcga
    13201 ctcccgggag cgtcggttgg cggtaaccag gtcgcgaacg tcgctcgact tgtagtcggt
    13261 gatgaccacc cgaccggctt cgtcacggtc gatcctgtcg tagcgcccgc gaatccggtc
    13321 cggcccgaag gagacggtga agtcctgctc gaccgccgtg gggcgtgccg gatcgcgctg
    13381 ctgctcggtc cagaagcgcc cgatcgcctc gtgcgcggcc gccttccgtg cctcctcgtg
    13441 ctcccgggtc aggaagccca ctgactccca gttcgagtcc agcgccgcgt gcagcccggc
    13501 ctcctccatc ggccggccgg cgagctggcg acggtggaag gcctgcgccg cggcatggag
    13561 cgcgcggccg tagaccatct ggtgcgagac cggggtcggg aggcggacga cgtgcgcgta
    13621 gcggtagcgc gccgggcagt ccaggtagtc gttcacctgg ccgtagctca gcatcagcgg
    13681 ccgatcgccc agggccggcc gcgccggggc cggcgcggtg ctcgtcgggg agccggagtg
    13741 gcgcgccagc cgctcgagca gggcgggctg cacggtctcg cgcggcgtcg ccggcggcag
    13801 gtcgagcgcc tccgtcacga actggctcgc ggcgcgcgag cgtcgccacc cctcgtcctc
    13861 ggcccagctg aggtgcagct gctccttcgc gcgcgtcatc ccgacgtaga agaggcgccg
    13921 ctcctcggcc gcgtgctggc ccgcgcccgg gtcgacggcg tctcgcacca gctcgtcggg
    13981 catctccagc gcgtcgccgc gctcgcgccc ggggaaccgg tcggcggtga cacccaccag
    14041 gaagacgacc gggaactcca ggcccttggc cttgtggtac gtcaggacgt ggaccgcgcc
    14101 gccgtcatcg gtgtcagcat ccgccgtcga cgggtcgtca cccgtctcga tcagcgtgtc
    14161 gagctgcgcg accaggaacg ggaggcggtc gtcccgcagc agcgccccct gccggcgcac
    14221 gatgtcgaag aagcgcgcca cgttcgccag ccgctcctcg cccgtctcgc gagcctcgtg
    14281 cgccagccgc gcgagccagc cggacgtggt gatgaagtgg tacagcagct caccgcagct
    14341 tcgctcggtg ctcatggcgc ggtgcgactc cagcgtcccc agcagccgcc cgaccacctc
    14401 gagcgcccgg gtcccgaagg gcgaaccgtc cggatcgcga gccgcctcgg cgagcgcatc
    14461 ggcaaggctg gagcgacgcc ggcgcgcgcg ggacagtgcg acggtcacgt cggccggagc
    14521 gaggccgaag atctcgctgg tcgccaggtc gtagaggctg accgagtcgt ccgggtcgtt
    14581 gaccgcgcgc aggaagctga tcaggacgcg cacttcggcc tggcggtaca gccccgccgt
    14641 gccgctgaag cgccagggga tgtgcgccat gttgagggcc cgcaggtagg gatccgcgtc
    14701 gcggttgccg cgcacgagga ccgcgtggtc accggcgcgg cggccggcac gcagggaggc
    14761 agcgatccgg tcggccaccg catcggcctc gtccgagacc gtcgtgtagc cggtgagccc
    14821 gatcggcccg gtctcgggtt ccggggtcgc gaagcgggac cgtgcccgca ggcgcttgtc
    14881 gatcccctcg cgcgcctcca gccggtcggg atcgttgtgg cggatgagcc gatgcgcggc
    14941 gtccaggatc ccctggcggc tgcggaagtt gtcgaccagc accacgctgg tcgccttcgg
    15001 gtacgtcgcg cggaagccga ggatgttgcc cagcgccgcg ccgcggaagc ggtagatgct
    15061 ctggtcgtca tcgccgacca cggtgacgtt gccgtgctcc gcggccagca gcttcaccag
    15121 ctcccactgc gcgtggttcg tgtcctggaa ctcgtccacc aggatgtacc gataccgacg
    15181 ccgctcctcc tcgagcaccg cgggatgctc ccgcaggagg cgaagggcga ggctgacctg
    15241 gtcaccgaag tcgagccgat cgttggcacg catcagctcc tcgtaggcgg cgtaggtcgc
    15301 ggcgagctcg gcgtgagtgg cggcgcgttc cgccagcgcc tcgtcctcgc gatgcgccgc
    15361 cgactccgcc cgcatgcgct cggccacctc gaggtaggcg gccggcggca cgtcctcgtc
    15421 gcgcaggcga ctgatcaggg tcaccatggc cgacaggaag cgcgtcgggt cgccgagcgg
    15481 gcggtatcgg tccagcggca gctcgaagag gtgctcgcgc aggaagatga cctgctccgc
    15541 gcgcgacagc acggtcgagc ggtccgagag cccggcctcc agggcgtgct cgcgcagcag
    15601 ccgatccccg aaggcgtgga aggtgctgat gaccgcgtcg ttgtgcccgt acggcaccag
    15661 gcgatcgacc cgttcggtca tctccagcgc cgcgcggtcc gtgaaggtca gcgccaggat
    15721 ctccgacggc agcgcccgct tctcggcgat cagccaggcg atgcgccgcg tgatgacggt
    15781 cgtcttgccg gtccccgccc cggcaacgat caggagcggc ccctcgccgt gcgtgaccgc
    15841 cttgcgctgc cgggccgtga ggtcggccaa cagcggcggc tggatgcgga ggtgcggccg
    15901 acgcggccgg gtcgccggga ccagctcctc gaagatcggc agctggtccg gggacggcac
    15961 gacgggtggc acgtcctcga tggtagcggc tggcgggttc accccgccgt tatcgcccgc
    16021 ttaccggaca ggtcgtctgt cgcgactccg cgggggaccg gtcgggcgtc gcggacggtt
    16081 gccgatggtc gccaggatcg cctccgcgtg gcggcggctc gtgcccagct cgtcgcggag
    16141 acgcgcgacg ctcaccgttc cgcgcatctc ctcgacgcgt gccgccaggc gcgcccaaac
    16201 gtccgagggg tagtcgatgc catccgcgac gtgccgcagc agcccgccgc tgcggagctg
    16261 gtcgagcacc gccggcggga tgcccaagcg cgccgccggt ccttcgacgc gcggtggcga
    16321 cgctcgcgcc tcccgcagcc cgctcagcag ccgctccacg cgctccgcca tctcgggggc
    16381 caggcccggc tcgcggtccg gcagccgcac gcggcggccc gtccgccgca gccgcccgtc
    16441 ctccaccatc gcctcgatca cgtcgcgcag gccgacgtcg tcgagctcga gcggcgtcgc
    16501 cccgcgatgc cgcggcggcg gccggcgccg cgcggcgtcg cgcacgcgcg cgaccagcgc
    16561 atcggtccgg atgtcggccg acagtggctg ctcgcggtgg aaccggcgca gcgccgccag
    16621 gatcaggcgt tcggcaaccc gccgctgctc cggctcgatc ggcccgcccg tcacggccgg
    16681 ggcgtcccct ccagcgcgat gcggatggcc tgcgactcct cctcggagag gtggtgctgc
    16741 gacgcccctc cggtcaccgg cttggcgaat acccgcgtgt tcgcgcgtcc gtgcaggctg
    16801 ctgagcacga cgccgtcgcg ctggtcgtcg agcatggc
//
LOCUS       DASTYP010000085        14657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_771243, whole genome shotgun sequence.
ACCESSION   DASTYP010000085 DASTYP010000000
VERSION     DASTYP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 14657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14657
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771243"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1561
                     /locus_tag="VFV59_04060"
     CDS             83..1561
                     /locus_tag="VFV59_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5325037.1"
                     /translation="MTVNPKKLRPYDEPPKRRRTLIPETPDSAATGFLVAAAVWFAIA
                     AGIGALGVLMRLAPFEISFPLGVFDLSFQLGERRVDAAFWNAAVYGWLSNAGFAALAF
                     MTPRLVGRRLVAERGLNVAMAVWNLSVAGGVAALYVFDLGPNSPLTAFPWLIDGGLAL
                     AALIVAGAFFGTVGAELRNAYVSTWFAAIAVLALLGLTGLMAVMGLLETFFDLPDLTV
                     GLGSVFIGRALSVLWLLGMAFAILHYVVPRATGRPLASAGLAMLSWVTWLVLAPLSAL
                     VVLQDTSVPYVFTTLGAVGTMLLFLPAVLTAVNLWFSMQGRWSLLFGLGPAAFAAVAL
                     VFLLATGLLDAIGALRSVDGLVRGTDWVDGAAIWTIFGTYTFAALALSEHAIPRLMRR
                     AWGGGALAGAQLWLTFAGATLAGMFLMFSGLAEGTLMASGTAAADITGQLVVYRAGAL
                     AGMGLLALAGLAAVVNLFLAYTSGEPAEYAVPGSSAPAAAGH"
     gene            1565..2053
                     /locus_tag="VFV59_04065"
     CDS             1565..2053
                     /locus_tag="VFV59_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5325038.1"
                     /translation="MTVHTPIESSSRGLPRWAIGLSVFVLLVGGVYLASNLTGENPQF
                     AIPGASAPVGSGGGGGEPDPAVGEQLVGQASPACTACHGPDLNGAGNFPSLHGVANGP
                     VSENLQQLGTDYPDNWAELWIDGTTPETEGLDRGGMPAFGDQFTPDQIASIVAYLKTL
                     GQ"
     gene            2082..2993
                     /locus_tag="VFV59_04070"
     CDS             2082..2993
                     /locus_tag="VFV59_04070"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEU5325039.1"
                     /translation="MTDPIARPERNVPVLIGADEMARIDEAAHRLGLAEDALMESAGA
                     AVTEVVLAELGRLAERVRGPGGPLARPPIVVVMCGPGNNGGDGFVVARRLASAGRRVV
                     VVLAAEASRLRGGAAAHNWTVLQAMASAGSLELFVAPAPELLVRLRERIAEATIVVDA
                     LLGSGASGPLREPISTAVDLINATRTHAQAAGRPVSVVAVDTPTRVDLTGGSRSTPVV
                     TADVTVTFHRAKAGFALDREARQLAGRYLVAPIGIPLEAEEGIVPPDGEWPPARITEI
                     TWHEPVERPDAAHRPGGGVAAGPGRTD"
     gene            3312..4754
                     /locus_tag="VFV59_04075"
     CDS             3312..4754
                     /locus_tag="VFV59_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5325040.1"
                     /translation="MFEYAEAAVGAALAAGARYADARVMDRRHESMNARNGEIEGLTQ
                     ESSVGLGVRALIGSGWGFASTADLSTQAARLAGAQAAAIARASASVPGKDLELTPVEV
                     RTDHWENPVEEDPLSVPLSEKGDLLVTVTRTMRDHGADVANAGYDAWDTTKWLVSSDG
                     HRIDQRIRECGARMDATANGERETQRRSYPGIRGQYGTRGWELVRQLDLPGNAARIAE
                     EARALLTAPECPSHDATDLIIGAEQMALQIHESVGHAVELDRILGWEAAFAGTSWLDL
                     SQLGSLRFGSDLMNITADATLPGALGSFGYDDEGTPAHPVDIVKDGIWVGVLSGRDSA
                     ALAGLDSSGGAVRGDGYNRLPMVRMTNVGLLPGSSSLDDMIAATDDGILMDTNRSWSI
                     DDRRLNFQFGCEIGWEIKDGKRGRMLKNPTYTGIGPRFWQSLDMLGNESEWTFWGTPN
                     CGKGQPGQVGHTGHPAVPCRFRGVRVGVRA"
     gene            4751..6109
                     /locus_tag="VFV59_04080"
     CDS             4751..6109
                     /locus_tag="VFV59_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018830665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5325041.1"
                     /translation="MTAVTIPSAEELLHVCDRVLALVGTDAEAEVTVTAGPESLTRFA
                     TSFIHQNVSDASRRIHLRVAVDGRVAEATANQTDPEALSRLVRGTLDAARLQPIDPGW
                     PGLAPPASAPDVDHWDQATADASPDDRAERVGDFVRVADDLESAGYCSTDGIVTAFAN
                     SAGQRLTGRATTAVLDGIARTGSSDGSARAASVRLADLDGAVVGAEAVRRARDAADAS
                     DLEPGRYEVVLSPACVVNMLGFLAIYGFNGRAVEEGRSFARLGEQQFDAAITLADDVT
                     HPMAVGVGFDAEGTPKRRVELVRAGTTVGLVNDRRTAKALGTDSSGNAVAGIGPFGAL
                     PANLVLQPGGVTAQELVGGVERGLLVTDFWYTRILDPRTQVVTGLTRNGVWLVEDGRI
                     VCPVTNLRFTQSFSEALGPGAVRGIGSDLTLVAGGVFGLGAYLVPSLHLASWNFTGGA
                     KG"
     gene            complement(6132..6611)
                     /locus_tag="VFV59_04085"
     CDS             complement(6132..6611)
                     /locus_tag="VFV59_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5325042.1"
                     /translation="MNTLYPCLWFDGNAEEAANFYASLLPDSHVDKVSRSPADTPSGP
                     AGMVLLVDFTLLGQRFQGLNGGSDFTFNEAVSFVVECDGQAEVDRLWDAFTADGGEPG
                     PCGWLKDRFGLSWQIVPRELGQLMEDPDPERARRAMEAMLQMGKIDIAEIRRAADAV"
     gene            complement(6670..7275)
                     /gene="pyrE"
                     /locus_tag="VFV59_04090"
     CDS             complement(6670..7275)
                     /gene="pyrE"
                     /locus_tag="VFV59_04090"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU5325043.1"
                     /translation="MTDASLRDASIEELFARSGALLDGHFALKSGRHAGRYLEKFLVL
                     QHPPIGIEIGRRMAGMLAPYRPTLVVGPTTGGVLLSFEVARQLGGGVRAAFAEPVASG
                     GRALRRGWPVSASERVVVVDDILTTGASLAETVAAVRDAGVDPLAAVVIVDRSVEPVD
                     VGVPVHSLGRIRIPSWTPEACELCAAGAPMVKPGSSTTADR"
     gene            complement(7272..8102)
                     /locus_tag="VFV59_04095"
     CDS             complement(7272..8102)
                     /locus_tag="VFV59_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325044.1"
                     /translation="MSRSTPSPRLTALRELAAEAELERRDFLYEATEQLRRFLDTNRD
                     RLREVGPIKLVDDEQDYLLYHPVDETWTTRLTYQDPADSEWYDEEQLVDTISELVELY
                     NPADIFSWLIEAARDAPSHLVPADELAREEELVEGGEEDWQAGLPAPQQETLAAQRLW
                     QLADAYRSQIATAQGQLLREFAANGEEVLRRTGDLTLLDEMDDILVLRSDGRFETSLD
                     LTDRPQDVLGDVEARRSGLTVTDARTIAAFYDPSDVLHWVTEALAERYPSVDFTAVYE
                     "
     gene            complement(8116..8442)
                     /locus_tag="VFV59_04100"
     CDS             complement(8116..8442)
                     /locus_tag="VFV59_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325045.1"
                     /translation="MPLMVTFFNVRKGRDTIFKRGMRHLFPRQARETERKYGVRFVGW
                     FNVTEGSTWNNVVILDLPNYAVLDQLYADPGMRGFGHRVAESVFDSKHTIFLRERMGP
                     DFVYKP"
     gene            complement(8442..8783)
                     /locus_tag="VFV59_04105"
     CDS             complement(8442..8783)
                     /locus_tag="VFV59_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325046.1"
                     /translation="MSLRYNEDLDPNEGPGFGCFITQAILFAIILLLIPIGLFTFNWP
                     VWVLVTLLVIDLILLFFVSMTSVFLLRVFFADRRRGRGRQTGVTKAPTRVITTEDGAP
                     TETEPMHDEGR"
     gene            complement(8853..10205)
                     /locus_tag="VFV59_04110"
     CDS             complement(8853..10205)
                     /locus_tag="VFV59_04110"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5325047.1"
                     /translation="MTDPTFGRLTDPPATLLLVGARVVDPGAGTDEVRDIGIIAGVLA
                     NPAAVPPGAERIEARGWIVAPGFCDLHTHLREPGGEEAETIASGTRAAAHGGFTTVCA
                     MPNTEPPLDTAERVAAVRRAAEGAAARVHVIAAATARRAGTTTSDVAALLDAGAVGLS
                     DDGASVPDAEVVRELLHAVGAQHPLIEHAEDPALAAGAVMRAGSTAARLGLLGWPPEA
                     EVRIVERDVALARATGGWVHVTHLSTAAALEVVRAARAEGLRVTCDVTPHHLALADDW
                     VGGERTFAWEEPEGRPKPPGAPYDGTCRVNPPLTTRQDARALLAGVADGSIDAIATDH
                     APHQQHRKLVPFADAAPGLIGLETALSLGLAAVQAGCLPLPALLAALATRPAALIGEE
                     RGLTIGSTADLVAFDPSGSWVVERDALASASWNTPLLGRALPGVVRLTVANGRVTYQS
                     "
     gene            complement(10202..11140)
                     /locus_tag="VFV59_04115"
     CDS             complement(10202..11140)
                     /locus_tag="VFV59_04115"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU5325048.1"
                     /translation="MISVPVAVAEQRHVVDVEGWSRAEIERMLDRAEEMRAARASRVY
                     RSLLDAETVGLAFFETSTRTRISFELAAKALGATVVDLQASSSSLSKGESLVDTLRTL
                     ERTGVTILVMRHASSGAPDLAVRATGLRVVNGGDGTHAHPTQALLDALTLRERFGSLD
                     GRLVAIVGDVAHSRVARSNIHALQALGARVRVAGPEPWIAGFDAWPGVETATSLADGI
                     RDADAVMTLRVQLERDAAASIGSLDEYIEAWRLDEERTARWAAPDAPILHPGPTNEGV
                     EITAELANGPRSLIGRQVENGVPIRMAVLATVAGAV"
     gene            complement(11137..11685)
                     /gene="pyrR"
                     /locus_tag="VFV59_04120"
     CDS             complement(11137..11685)
                     /gene="pyrR"
                     /locus_tag="VFV59_04120"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU5325049.1"
                     /translation="MTERRVLGADDIRRATTRIAHEIVERHGGIDGLVLVGIRSRGVP
                     LARRLAALIEQHEGGSVPIGSLDITYYRDDLTRVAHAPIVRRSDLEAEVEGRTVVLVD
                     DVLYTGRTVRAALDALTDRGRPHAVRLAALVDRGHRELPIRPDFVGKNLPTSPDEIVH
                     VRLLETDDVDEVVIERSNGGAS"
     gene            complement(11769..13490)
                     /locus_tag="VFV59_04125"
     CDS             complement(11769..13490)
                     /locus_tag="VFV59_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5325050.1"
                     /translation="MTMNRHEPFEEFISASLAGDITDDERRHLDAHLDSCAQCRDTLR
                     AFADQRRIIAGLRQVSPPRDLGARVRGGIEAGAFAPLPWWRRPAVIFAGVGGGLAAVA
                     GVLLALVILGALPDQSAVGNSPTPSVSLSAAPSASVVASSTPVPASPSAAPSASLQPS
                     PTAVPTPEPSPEPDAYLALTGPFDNLLLTARDGATGETLVEAETVPPGGPTEGELSPD
                     GDWFAYWTPVGEKGTWEIFATRIGEASGRDSTVAVGGTAISIESQAGGSFLDHTAWSP
                     DGRYLAFTVSGMGDGSVDAFVFEPQTGVATRLTDVGTAYAGSWVENPDEPGSYDLWVS
                     VAGPDPVSYLVPVLDDAGAPLEDIDPAAEALATLDGVFQPTVSPNGAFVIYWRGTMSQ
                     SGADWTLADGGAPYLAEHRWEAGGTASFENERQLFADLTIGRSAFASGAVTWGPDSDA
                     YAFWDLQWTGTSQGTNGVEYPDPQRVYFSHATDARGITQAHALDVDDIPADTRVVDVA
                     IAPTGRHLAITVARPSAGLMDPPHADLLLVTRNTGSQPDEVLVLGSGDDGWFGPAGYD
                     LRDERAD"
     gene            complement(13487..14020)
                     /locus_tag="VFV59_04130"
     CDS             complement(13487..14020)
                     /locus_tag="VFV59_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5325051.1"
                     /translation="MSGRAETPERAFDRLFEEYSAPIYNYVLRMVADADRAADITQDT
                     FIKAYRKLDTLTDASSTRAWLYRIATNTAIDEMRRRRMVRPMHDDQPAIANRPDPRPG
                     PESQVMAGTLDERVQRALARLRPNHRQCLLLSDLEDMSPRQIGEIMDLSYAAVRTLLC
                     RARGEMRRALAAEGFDR"
     gene            complement(14142..>14657)
                     /locus_tag="VFV59_04135"
     CDS             complement(14142..>14657)
                     /locus_tag="VFV59_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU5325052.1"
                     /translation="QHLNVELYAIPQVPHWVAEEIGGGARQMIKARRCVWCALAEAEE
                     ENAERLVFSDHHAVVFAPAASRSAFEMMVVPRTHAADFTSLDDEAVADTTATLQRALH
                     ALDALGDPPYNLFLHTSPAGERLDTTFHWHWEIHPRLRVIAGLERATALAVNPAAPEY
                     AAAVLREHLTA"
BASE COUNT         1961 a         5228 c         5341 g         2127 t
ORIGIN      
        1 ttgcacggcg ttgggtacaa tgcggcggcc tactccgcca ctcggcctgc gagcgaagcc
       61 ccgggagggt ttgacgcgcc ccatgacggt gaaccccaag aagttgcgcc cgtacgacga
      121 gccgccgaag cgacgccgca cactcatccc tgagacgccg gacagcgctg cgaccggctt
      181 cctggtggcc gcagccgtct ggttcgcgat cgccgcgggc atcggtgccc tgggggtgct
      241 catgcggctg gcgccattcg agatctcgtt cccgctgggc gtcttcgacc tctccttcca
      301 gctcggtgag cggcgcgtcg acgccgcttt ctggaacgcg gccgtctacg gctggctgtc
      361 gaacgcgggc ttcgcggcgc tggccttcat gacgccccgg ctggtcggcc gccggctggt
      421 cgcggagcgt ggcctgaacg tggcgatggc agtctggaac ctgtccgtcg ccggcggggt
      481 cgccgcgctc tacgtcttcg acctgggccc gaactcgccg ctgaccgcct tcccctggct
      541 gatcgacggt gggctggccc tcgccgcact gatcgtggcg ggcgccttct tcggcacggt
      601 cggcgcggag ctccgcaatg cctacgtgag cacctggttc gccgccatcg ccgtcctggc
      661 gctgctcggc ctgaccggcc tcatggcggt gatggggctg ctcgagacct tctttgacct
      721 gccggacctg acggtcgggc tgggatcggt cttcatcggc cgagccctga gcgtcctgtg
      781 gctgctgggc atggcattcg cgatcctgca ctacgtcgtg ccgcgcgcca ccggccgacc
      841 gctcgcatcg gccgggctgg ccatgctgag ctgggtcacc tggctggtgc tggccccgct
      901 gtcagcgctc gtcgtcctgc aggacacgag cgtgccgtac gtgttcacca cgctcggtgc
      961 cgtgggcacc atgctgctgt tcctgccggc ggtgctcacc gcggtcaacc tgtggttctc
     1021 gatgcagggt cgctggtcgc tcctcttcgg cctcggcccg gccgcgttcg ccgccgtggc
     1081 gctcgtcttc ctgctggcca ccgggctgct cgacgcgatc ggcgccctcc gatcggtcga
     1141 cggcctggtc cgcggcacgg actgggtcga cggtgcggcg atctggacga tcttcggcac
     1201 gtacacgttc gcggcgctgg ccctctcgga gcacgccatt ccgcgcctga tgcgacgcgc
     1261 ctggggtggc ggggcgctgg ccggtgcaca gctgtggctc acgttcgccg gcgccaccct
     1321 ggccggcatg ttcctcatgt tctccggcct ggccgagggc acgctcatgg cgtccggcac
     1381 ggccgccgcg gacatcaccg gccagctcgt cgtgtaccgg gccggggcgc tcgccgggat
     1441 ggggctcctg gccctcgccg gcctggccgc ggtggtgaac ctcttcctcg cctacaccag
     1501 cggtgagccg gccgagtacg ccgtgcccgg ctcgtccgcg ccagccgcag caggacactg
     1561 accgatgacc gttcacaccc cgatcgaatc gagctcgcgc ggcctgccgc gctgggccat
     1621 cggcctctcc gtcttcgtcc tcctggtcgg cggcgtgtac ctggcatcca acctgaccgg
     1681 ggagaacccg cagttcgcca tccccggtgc cagcgcgccg gtcggttcag gcggcggtgg
     1741 tggcgagccg gacccggccg ttggagagca gctcgtcggc caggcatccc cggcctgcac
     1801 cgcgtgccac ggcccggatc tgaacggtgc gggcaacttc ccgagcctgc acggcgtggc
     1861 gaacggaccc gtgagcgaga acctgcagca gctcgggacc gattaccccg acaactgggc
     1921 cgagctgtgg atcgacggca cgactcccga gaccgagggc ctcgaccgcg gcggcatgcc
     1981 ggcgttcggc gaccagttca cgccggacca gatcgcctcg atcgtcgcgt acctgaagac
     2041 gctcggccag tagagccagc agggcccggc cggccacggc catgaccgat cccatcgcgc
     2101 gccccgagcg caacgtcccc gtcctcatcg gcgccgatga gatggcgcgc atcgacgagg
     2161 ccgcgcaccg cctggggttg gccgaggacg cgctgatgga gtccgccggc gccgccgtca
     2221 ccgaagtcgt cctggcggag ctcggccggt tggccgagcg ggttcgtggc cccggcggtc
     2281 cgctggcgcg cccgccgatc gtcgtggtga tgtgcgggcc gggcaacaac ggcggtgacg
     2341 ggttcgtggt cgcgcgccgg ctggccagcg cggggcgtcg cgtagtggtg gtgctggccg
     2401 ccgaggcctc gcgcctccgc ggcggtgcgg ccgcccacaa ctggacggtg ctccaggcca
     2461 tggcctccgc gggctcgctg gagctgttcg tcgcgccggc gccggagctg ctggtccggt
     2521 tgcgcgagcg gattgccgaa gcgaccatcg tcgtcgacgc gttgctgggc tccggtgcca
     2581 gcggcccctt gcgtgagccc atctccaccg cggtggacct gatcaacgcg acgcggacgc
     2641 acgcgcaggc cgccggtcga ccggtgtcgg tggtggccgt ggacacgccc acccgcgtcg
     2701 atctcaccgg cggctcgcgg tcgacgcccg tggtcacggc cgatgtgacg gtcacgttcc
     2761 accgcgccaa ggcgggcttc gccctcgacc gtgaggcgcg gcagctggcc ggccgctatc
     2821 tggtggcgcc gatcggcatc ccgttggagg cggaggaggg gatcgtcccg cccgacgggg
     2881 agtggccgcc ggcgcggatc accgagatca cctggcacga gccggtggag cgccccgacg
     2941 ccgcgcaccg gccgggcggg ggcgtggcgg ccggacccgg gcggactgac tagcgactcg
     3001 acggccgctc ggtctcgctg cggcgtggcc gctgacgcgt ccgaattcac gccacgcgtg
     3061 cgtcaggcgt gggccggccg gctgagcatg gccgacgcgg cctctggccc gtccgcattc
     3121 acaggtcgcg tgaattcggc ccctccaggg caccaggtca cgccctggac gcgtcacgtg
     3181 cgcactccat gtgaatctga ccggccgcgc gcgccctcgg cgtagcgtcg cgcggaccga
     3241 tcgcgcgaac ggcgcgggac gaacgaccgc gctccgccac gcaccaccgt tagcggcgct
     3301 aaccttcgag gatgttcgag tacgccgagg ccgccgtcgg agcggccctg gcggcggggg
     3361 cccgatacgc cgacgcgcgg gtcatggacc gtcgccacga gtcgatgaac gcccgcaacg
     3421 gcgagatcga ggggctgacg caggagagct ccgtcgggct cggcgtgcgc gccctgatcg
     3481 ggtccggctg gggcttcgcc agcaccgccg acctctcgac acaggcggcc cgcctcgcgg
     3541 gcgcgcaggc ggccgcgatc gcgcgcgcca gcgcatcggt cccggggaag gatctggagc
     3601 tcaccccggt ggaggtgcgc accgaccact gggagaaccc cgtcgaggag gatccgctgt
     3661 ccgttccgct ctccgagaag ggcgatctgc tggtgaccgt cacccgcacc atgagggacc
     3721 acggggccga tgtggcgaac gccgggtacg acgcgtggga cacgacgaag tggctcgtca
     3781 gcagcgacgg gcatcggatc gaccagcgca tccgggagtg cggcgcacgc atggacgcga
     3841 ccgccaacgg cgagcgtgag acacagcggc gcagttaccc gggcatccgc gggcagtacg
     3901 gcacgcgcgg ctgggagctg gtgcggcagc tggacctgcc gggcaacgcg gcgcggatcg
     3961 ccgaagaggc tcgggcgctg ctgacggcgc ccgagtgccc cagccacgac gcgaccgacc
     4021 tgatcatcgg agccgaacag atggcgctgc agatccacga gtcggtcggc cacgccgtgg
     4081 agctggaccg catcctgggc tgggaggcgg cctttgccgg cacctcctgg ctggacctgt
     4141 cgcagctggg gtcactccgc ttcgggtcgg acctcatgaa catcaccgcg gatgccacgc
     4201 tcccgggggc tctcgggtcc ttcgggtacg acgacgaggg caccccggcg catccggtcg
     4261 acatcgtcaa ggacgggatc tgggtcggcg tcctgtcggg ccgcgactcc gcggccctgg
     4321 ccggcctgga cagctccggt ggggcggtcc gcggcgacgg gtacaaccgc ctgccgatgg
     4381 tgcgcatgac gaacgtcggt ctcctcccgg gctcctcgag cctcgacgac atgatcgcgg
     4441 cgaccgacga cggaatcctg atggacacga accgatcctg gtcgatcgac gaccggcggc
     4501 tgaacttcca gttcggctgc gagatcggct gggagatcaa ggacggcaag cgggggcgga
     4561 tgctgaagaa cccgacctac accggcatcg ggccgcgctt ctggcaatcg ctcgacatgc
     4621 tgggcaacga gtccgagtgg accttctggg gcacgcccaa ctgcggcaag ggccagcccg
     4681 ggcaggtcgg ccacaccggt caccccgccg ttccctgccg cttccggggc gtgcgggtgg
     4741 gggtgcgggc atgaccgctg tcaccatccc gtccgccgag gagctcctgc acgtctgcga
     4801 tcgcgtcctc gccctggtcg ggaccgatgc ggaggccgag gtcaccgtca ccgcgggccc
     4861 ggagtcgctc acgcgcttcg cgaccagctt catccaccag aacgtctccg acgccagccg
     4921 ccggattcac ctgcgcgtcg ccgtcgacgg gcgcgtggcc gaagccaccg ccaaccagac
     4981 cgatcctgag gcgctctcac gcctcgttcg tgggaccctc gatgcggcgc ggctgcagcc
     5041 gatcgatccc ggctggccgg gcctggcgcc gccggcgtcg gcgcccgacg tcgaccactg
     5101 ggaccaggcc acggccgatg cctcgcccga cgaccgcgcc gagcgcgtgg gcgacttcgt
     5161 gcgggtggcc gacgacctcg agtcggcggg gtactgctcc accgacggca tcgtcacggc
     5221 cttcgccaac tcggccgggc agcggctgac cggccgggcc accacggccg tgctcgacgg
     5281 catcgcgcgc accggctcct ccgacgggtc ggcgcgggcg gcctcggtac gcctggccga
     5341 cctggacggg gcggtcgtcg gggccgaggc ggtgcgccgg gcgcgcgacg ccgccgacgc
     5401 ctccgatctc gagccgggcc gctacgaggt cgtcctctcc ccggcctgcg tcgtcaacat
     5461 gctcggcttc ctggccatct atggcttcaa cggccgcgcc gtggaggagg gccgttcctt
     5521 tgcgcgcctg ggtgagcagc agttcgatgc cgccatcacg ctggccgacg acgtgacgca
     5581 cccgatggcc gtcggggtgg gcttcgacgc cgagggcacg cccaagcgcc gcgtggagct
     5641 ggtccgtgcc ggcaccacgg tcggcctggt caacgaccgc cgcaccgcga aggcgctcgg
     5701 caccgactcg tcgggcaatg cggtggccgg catcggccca ttcggtgcgc tgcccgcgaa
     5761 cctcgtgctg cagcctggcg gcgtcacggc ccaggagctg gtcggcggcg tcgagcgcgg
     5821 gctgctggtg accgacttct ggtacacccg catcctcgac ccgcggacgc aggtggtcac
     5881 cgggctcacc cgcaacggcg tgtggctggt ggaggacggt cgcatcgtgt gtcccgtgac
     5941 gaacctgcgg ttcacgcagt ccttctccga ggcgctgggt ccgggggccg tgcgtggcat
     6001 cggctccgac ctcaccctgg tcgccggcgg cgtcttcggc ctgggggcct acctggtgcc
     6061 ctcgctgcac ctggcctcgt ggaacttcac cggcggcgcg aagggctgag accgcgcaat
     6121 caccgcgtca gctagacggc gtcggccgcg cggcggatct cggcgatgtc gatcttgccc
     6181 atctgcagca tcgcctccat cgcgcggcgg gcgcgctcgg ggtccgggtc ctccatcagc
     6241 tgcccgagct cgcgcggcac gatctgccac gacaggccga agcggtcctt cagccagccg
     6301 cacggaccgg gctccccgcc gtcggcggtg aaggcgtccc agaggcggtc gacctcggcc
     6361 tgcccgtcgc actcgacgac gaaggagacg gcctcgttga aggtgaagtc cgagccgccg
     6421 ttcagtccct ggaagcgctg accgaggagg gtgaagtcga ccagcaggac catgccggcg
     6481 gggccgctcg gcgtatcggc gggggatcgc gagaccttgt ccacgtggct gtccggcagg
     6541 aggctggcgt agaagttggc ggcctcctcc gcgttgccgt cgaaccagag gcaggggtac
     6601 agcgtgttca tcgggtgctc ccgtggtgca ggggttcgtc gccactagga cgagccggcg
     6661 tcgaccgact catcggtcag cggtggtgct gctgcccggc ttgaccattg gcgcgccggc
     6721 ggcgcacagc tcgcaggcct ccggcgtcca cgatgggatc cggatccggc caagcgaatg
     6781 gaccggcacc cccacgtcca ccggctcgac cgagcggtcg acgatgacga cggccgccag
     6841 cgggtcgacg cccgcatcgc ggacggccgc gacggtctcg gccagggacg cgccggtggt
     6901 caggatgtcg tccacgacca ccacccgctc ggatgccgac accggccatc ctcgacgcag
     6961 cgcccgtccg ccggatgcaa ccggttccgc gaacgcggcc cgcacgcccc cgcccagctg
     7021 ccgtgccacc tcgaacgaca gcagcacgcc gccggtggtg ggtcccacga ccagggtcgg
     7081 ccggtacggc gccagcatcc cggccatgcg ccgcccgatc tcgatgccga tcggcgggtg
     7141 ctgcaggacc aggaacttct cgaggtagcg gccggcgtgg cgcccggact tcagcgcgaa
     7201 gtgcccgtcc agcagtgcgc cggagcgcgc gaatagctct tcgatgctgg cgtcgcgcag
     7261 cgacgcatcg gtcattcgta gacggccgtg aagtcgaccg aggggtagcg ctcggcgagg
     7321 gcctcggtga cccagtggag gacgtcggac gggtcgtaga aggccgcgat cgtgcgggca
     7381 tcggtcacgg tgagccctga gcggcgcgcc tcgacgtcac ccaggacgtc ctgcggtcgg
     7441 tccgtaaggt ccagcgaggt ctcgaagcga ccgtcgctgc gcaggaccag gatgtcgtcc
     7501 atctcgtcga gcagggtgag gtcgcccgtg cgacgcagca cttcctcacc gttggcggcg
     7561 aactcgcgca gcagctggcc ctgggcggtg gcgatctgcg accgataggc atcggccagc
     7621 tgccagaggc gctgagcggc cagcgtctcc tgctggggag cggggagccc ggcctgccag
     7681 tcctcctcgc cgccctcgac cagctcctcc tcgcgggcca gctcgtcggc cgggacgagg
     7741 tgggagggcg cgtcgcgggc ggcctcgatc agccaggaga agatgtcggc cgggttgtac
     7801 agctcgacca gctcgctgat ggtgtcgacc agctgctcct cgtcgtacca ctcgctgtct
     7861 gccgggtcct ggtaggtcag ccgcgtcgtc cacgtctcgt cgaccgggtg gtacagcagg
     7921 tagtcctgct cgtcgtcgac cagcttgatg ggccccacct ctcgcagccg gtcgcggttc
     7981 gtgtccagga atcgccgcag ctgctccgtg gcctcgtaca ggaagtcgcg gcgctccagc
     8041 tcggcctcgg cggcaagctc gcgcagcgcg gtgaggcgcg gggagggcgt ggatcggctc
     8101 atcggtccgg ttccttcacg gcttgtagac gaagtcgggt cccatgcgct cgcgcaggaa
     8161 gatcgtgtgc ttggagtcga acacggactc tgccacgcgg tggccgaagc cgcgcatgcc
     8221 cggatcggcg tacagctggt cgaggaccgc gtagttcggc aggtccagga tcaccacgtt
     8281 gttccaggtc gagccctccg tcacgttgaa ccagccgacg aagcgcacgc cgtacttgcg
     8341 ctccgtctcg cgggcctggc gggggaagag gtggcgcatg ccgcgcttga agatcgtgtc
     8401 gcggcccttg cggacgttga agaaggtcac catcagcggc atcagcggcc ctcgtcgtgc
     8461 atcggttcgg tctccgtcgg tgctccgtcc tcggtggtga tcacgcgggt gggcgccttc
     8521 gtgacaccgg tctggcgtcc tcgacctcgc cggcgatcgg cgaagaagac gcgcagcagg
     8581 aagacgctgg tcatgctgac gaagaacagc aggatgaggt cgatgacgag cagcgtgacg
     8641 agcacccaca ccggccagtt gaaggtgaac aggccgatcg ggatgagcag caggatgatc
     8701 gcgaacagga tcgcctgcgt gatgaagcag ccgaagccgg gaccctcgtt cgggtccagg
     8761 tcctcgttgt agcgaagcga caagccattc ctcgcatggg cgttcggtgc gtggcggtcc
     8821 gatcgtccca caatcggcgg gcgtccgccc gctcaggact gatacgtcac gcggccgttc
     8881 gctaccgtga gccgcacgac gcccggcagc gcgcgaccca gcaggggcgt gttccatgag
     8941 gccgatgcga gcgcgtcgcg ctcgacgacc cagctccccg atggatcgaa cgcgacgagg
     9001 tccgcggttg agccgatggt caggccgcgc tcctcgccga tgagcgctgc gggacgcgtc
     9061 gccagcgctg ccagcagggc gggcaggggg aggcagcccg cctgaacggc ggccaggcca
     9121 agggacagcg cggtctcgag gccgatgagg cccggcgcgg catccgcgaa tggcaccagc
     9181 ttgcggtgct gctgatgcgg cgcgtggtcg gtggcgatgg cgtcgatgga gccgtcggcg
     9241 acgccggcga gcagcgcacg ggcatcctgg cgggtggtga gcggcgggtt gacgcggcag
     9301 gtgccgtcgt agggggcgcc gggtggcttc ggccgccctt ccggctcctc ccaggcgaag
     9361 gtccgttcgc cgccgaccca gtcatcggcg agcgccaggt ggtgtggcgt gacgtcgcag
     9421 gtcacccgca gcccttcggc gcgcgcggcg cgaacgacct cgagcgcggc cgccgtcgac
     9481 aggtgggtca cgtgcaccca tccgcccgtc gcacgagcca gcgcgacgtc gcgctccacg
     9541 atccgaacct cggcctccgg cggccacccg aggaggccca ggcgagcggc agtcgacccg
     9601 gcccgcatga ccgctcctgc agccaatgcc ggatcctccg cgtgctcgat cagcgggtgc
     9661 tgcgcgccga cggcatgcag cagctcgcgg acgacctccg cgtcggggac cgaggcgccg
     9721 tcgtccgaca agccgaccgc gccggcgtcc aggagtgcgg cgacatcgct ggtcgtcgtg
     9781 ccggcacgtc gggcggtggc cgcggcgatc acgtgcaccc gggccgcggc accctccgcg
     9841 gcgcgccgga ccgccgccac tcgctcggcc gtgtccagag gcggctccgt gttcggcatg
     9901 gcgcacacgg tcgtgaagcc gccgtgagcc gccgcccgcg tcccgctggc gatggtctcg
     9961 gcctcctcgc caccgggctc acgcaggtgc gtgtgcaggt cgcagaaccc cggagcgacg
    10021 atccagccgc gtgcctcgat ccgctcggcg ccgggcggca cggcggcggg gttggcgagg
    10081 acgcccgcga tgatcccgat gtcgcgcacc tcgtcggtcc cggcgcccgg gtcgacgacg
    10141 cgcgcgccga ccagcagcag cgtggccggc gggtcggtca ggcgcccgaa ggtcggatcc
    10201 gtcacaccgc gccggcgacg gtggcgagga cggccatccg gatcggcacg ccgttctcga
    10261 cctggcggcc gatgaggctc cgcggcccgt tggccagctc ggcggtgatc tccaccccct
    10321 cgttggtcgg tccggggtgc aggatcggcg cgtccggcgc cgcccagcgc gccgtgcgct
    10381 cctcgtccag gcgccaggcc tcgatgtact cgtcgagcga cccaatgctg gcggcggcat
    10441 cgcgttccag ctggacgcgc agggtcatga ccgcgtcggc gtcgcggatg ccatcggcca
    10501 ggctggtcgc ggtctcgacg cccggccatg cgtcgaatcc ggcgatccat ggctcgggac
    10561 cagcgacccg cacgcgggca cccagcgcct gcagggcgtg gatgttcgac cgcgccacgc
    10621 gcgagtgcgc cacgtcgccg acgatggcga ccaggcgccc gtcgagcgag ccgaaccgct
    10681 cgcgcagcgt cagcgcgtcc agcagcgcct gggtggggtg ggcgtgggtg ccgtccccgc
    10741 cgttcacgac gcgcaggccg gtggctcgca cggccagatc gggggcgccc gacgaggcgt
    10801 gacgcatgac caggatcgtg accccggtgc gctcgagcgt tcgtagcgtg tcgaccagcg
    10861 actcgccctt gctgaggctg ctgctcgacg cctgcaggtc gacgacggtg gcgccgagcg
    10921 ccttggccgc gagctcgaag ctgatccgcg tccgcgtcga ggtctcgaag aaggccagcc
    10981 cgacggtctc ggcgtccagc agcgaccgat agacgcgcga cgcccgcgcc gcgcgcatct
    11041 cctccgcccg gtcgagcatg cgctcgatct cggctcgact ccatccctcc acgtccacga
    11101 cgtggcgctg ctctgcgacc gccaccggga ccgagatcac gatgcacctc cgttcgaccg
    11161 ttcgatgacc acctcgtcca cgtcatcggt ctccagcagc cggacgtgga cgatctcgtc
    11221 cgggctggtc ggcaggttct tgcccacgaa gtcggggcga atcggcagct cgcggtgccc
    11281 gcggtcgacc agcgcggcca ggcggacggc gtgcggccgt ccccggtcgg tgagagcgtc
    11341 gagcgccgcg cgcacggtgc ggccggtgta gaggacgtcg tccaccagga cgacggtcct
    11401 gccttcgacc tccgcctcga ggtcggaccg gcggacgatc ggcgcgtggg ccacccgcgt
    11461 caggtcgtcg cggtagtacg tgatgtccag cgacccgatc ggcaccgatc cgccttcgtg
    11521 ctgctcgatc agcgcggcca ggcgacgggc aaggggaaca ccgcgggagc ggatgccgac
    11581 gagaacgaga ccatcgatgc cgccgtgtcg ctccacgatc tcgtgagcga tgcgcgtcgt
    11641 cgcgcggcgg atgtcatcgg cgccgaggac ccggcgttcg gtcaatgcgg ctctccttgg
    11701 cggcctcacc ggaccgcgtt aaaggcttcg cgcggcagta tacgaccgcg ggccgatcgc
    11761 cggcggcgct agtccgcccg ctcgtcgcgc aggtcgtagc cggcgggacc gaaccagccg
    11821 tcgtcgccac tgcccaggac cagcacctcg tccggctggc ttccggtgtt gcgcgtgacg
    11881 agcagcaggt cggcgtgggg cggatccatc aggccggcgc tcgggcgtgc caccgtgatg
    11941 gccaggtgac ggccggtcgg cgcaatcgcc acgtcgacga cgcgggtgtc ggcgggaatg
    12001 tcgtcgacgt cgagagcgtg cgcctgggtg atgccgcgcg catcggtcgc gtggctgaag
    12061 tacacacgct gcgggtcggg gtactccacc ccgttggtgc cctgcgaggt gccggtccac
    12121 tgcaggtccc agaaggcata cgcgtcgctg tccgggcccc acgtcacggc gcccgaggcg
    12181 aaggcgctgc ggccgatggt caggtcggcg aagagctgcc gctcgttctc gaagctggcg
    12241 gtcccccccg cctcccagcg gtgctcggcg aggtacggag ccccgccatc ggcgagcgtc
    12301 cagtcggccc cggactggga catggtgccg cgccagtaga tgacgaacgc gccgttcggg
    12361 ctcaccgtcg gctggaagac gccgtcgagc gtcgccagcg cctccgccgc gggatcgatg
    12421 tcctcgagcg gcgcacccgc gtcgtcgagg acgggcacca ggtagctcac cggatcgggc
    12481 ccggccacgc tgacccacag gtcgtagctg ccgggctcat cggggttctc gacccagctg
    12541 ccggcgtagg cggtgccaac gtcagtcagg cgcgtcgcca cgccggtctg cggctcgaac
    12601 acgaacgcgt ccacgctgcc gtcgcccatg ccgctcaccg tgaacgccag gtagcggccg
    12661 tccggagacc aggctgtgtg gtccaggaag gagccaccgg cctggctctc gatggagatg
    12721 gcggttccgc ccacggcgac ggtggagtca cgcccggacg cctcaccgat gcgcgtcgcg
    12781 aagatctccc atgtcccctt ctcgccgacc ggcgtccagt acgcgaacca gtcgccgtcg
    12841 ggtgacagct cgccctcggt cgggccgccc ggcggcaccg tctccgcctc gaccagcgtc
    12901 tctcccgtcg cgccgtcgcg cgcggtcagc agcaggttgt cgaacgggcc ggtcagtgcc
    12961 aggtaggcgt caggctcggg cgagggctcg ggagtcggca ccgcggtcgg gctcggctgg
    13021 agcgaggcgc tgggtgccgc cgaagggctg gcggggaccg gcgtcgagga cgccaccacc
    13081 gatgccgagg gcgcggcgga gagcgagacg ctcggcgtcg gcgagttccc caccgccgac
    13141 tggtccggca gcgcgccgag gatcaccagc gcgagcagca caccggcgac cgcggccagc
    13201 ccaccgccga cgccggcgaa gatcacggcc ggacgccgcc accagggcag cggcgcgaac
    13261 gcgccggcct cgatgccgcc gcgcacgcgc gcgccgaggt cgcgcggcgg cgacacctgg
    13321 cgcaggccgg cgatgatgcg gcgctggtcg gcgaaggcac gcagcgtgtc gcggcactgc
    13381 gcgcacgaat cgaggtgcgc gtccaggtgg cggcgctcgt catcggtgat gtcgccggcc
    13441 agggaggcgc tgatgaactc ctcgaacggc tcgtggcggt tcatcgtcat cggtcgaagc
    13501 cctccgccgc gagggcgcgg cgcatctcac cgcgggcgcg gcacagcagc gtgcggacgg
    13561 cggcgtacga cagatccatg atctccccga tctgccgagg gctcatgtcc tcgaggtcgc
    13621 tgagcaacag gcattggcgg tggttgggcc tcagccgggc cagggcgcgc tgcacgcgct
    13681 cgtcgagcgt gccggccatg acctgcgact ccggcccggg acgcgggtcg gggcgattgg
    13741 cgatcgcggg ctgatcgtcg tgcatcggcc ggaccatgcg ccgccggcgc atctcgtcga
    13801 tcgccgtgtt ggtcgcgatc cggtagagcc aggcgcgggt cgaggatgca tcggtcaggg
    13861 tgtcgagctt gcggtaggcc ttgatgaagg tgtcctgcgt gatgtcggcg gcacggtcag
    13921 catcggccac catgcgcagc acgtagttgt agatgggcgc cgagtactcc tcgaacagcc
    13981 gatcgaacgc ccgctccggc gtctcggctc gaccgctcat gacctgcgga ctccacggca
    14041 tctggccggg aatcgccatc gactgcacct cgtcctccgc acacggctcc ccgcttcggt
    14101 gccggtgtga cgaacgcctc cccgcatctg ttaccgatgc ctcacgccgt gaggtgctcg
    14161 cggaggaccg cggcggcgta ctccggggcc gccgggttca cggccagggc ggtcgcgcgc
    14221 tccagcccgg cgatgacgcg caggcgtgga tggatctccc agtgccagtg gaaggtcgtg
    14281 tccaggcgct cgccggccgg cgacgtgtgc aggaacaggt tgtacggcgg gtcacccagc
    14341 gcgtcgaggg cgtggagcgc ccgctggagc gtggccgtcg tatcggcgac ggcctcgtcg
    14401 tccaggctcg tgaagtcggc ggcgtgcgtc cgcggcacga ccatcatctc gaaggcggac
    14461 cggctggcgg cgggcgcgaa gacgacggcg tggtggtcgc tgaagaccag gcgttcggcg
    14521 ttctcctcct cggcctcggc cagcgcgcac cacacgcagc gacgcgcctt gatcatctgg
    14581 cgcgcgccgc cgccgatctc ctcggcgacc cagtgcggga cctgcgggat ggcgtacagc
    14641 tccacgttga ggtgctg
//
LOCUS       DASTYP010000086        10508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1098990, whole genome shotgun sequence.
ACCESSION   DASTYP010000086 DASTYP010000000
VERSION     DASTYP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098990"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1347
                     /gene="clpX"
                     /locus_tag="VFV59_04140"
     CDS             31..1347
                     /gene="clpX"
                     /locus_tag="VFV59_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5325053.1"
                     /translation="MTTKSKVPYRCSFCGKSQEQVRKLIAGQGVYICDECINLCQEII
                     DEEMLDAPRNGAKAQNTPLPSPRKIKDQLDQYVISQEAAKKVLSVAVYNHYKRVNAGH
                     TVDDVELGKSNVLLVGPTGSGKTLLAQTLAKVLDVPFCIADATSLTEAGYVGEDVENI
                     LLRLIQAADFDVQRAQRGIIYIDEIDKIARKTDNPSITRDVSGEGVQQALLKILEGTV
                     ANVPPQGGRKHPHQDFIQIDTTNILFICGGAFEGLEKIIEERVGRRSIGFGSQGAKQT
                     KDERRQALFDKLMPEDLLKYGLIPEFVGRLPVVVSLSALAKEDLVKVLTEPKNAVTRQ
                     FTKFLSLDKVELVFTPDALEATAKLALDRKTGARALRTIVEEALLDVMYDIPERTDVK
                     KVVITAETIEHGQPPMLVTETDAETEPRRRGRGRPSRDEEPEEESA"
     gene            1365..1886
                     /locus_tag="VFV59_04145"
     CDS             1365..1886
                     /locus_tag="VFV59_04145"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5325054.1"
                     /translation="MDRSSALHAALRLSVEHLWWTNRRLLVAAARLPDGAWEADHVTT
                     RSLRATLVHELDVEWSWRLALEGRPVEEWGSDAEMRPEDFPSVESLRERWSREADALR
                     QWVEGLSEDELLAETRPGLSSRPLPRWMFIQHLVSHGAQQAADAATLLTSAGASPGDI
                     GFLEYIGESHRGR"
     gene            complement(1902..2807)
                     /locus_tag="VFV59_04150"
     CDS             complement(1902..2807)
                     /locus_tag="VFV59_04150"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEU5325055.1"
                     /translation="MPAFPDLPDSVETVLRIGLIALVALAAFVGVRFAIQVAVRHLLE
                     RRSAEGGGESDTPVELERRMNTVGRLLVRVAGAIVAVIAVLMVLNEFGVDIGPAVAGL
                     GVVGIAVGLGAQTLVKDWLAGIFIVLENQYSQGDVVRIAGVDGVVEDFSLRRTTLRDL
                     DGTVHSVPNGQISVASNLTRLWARVNLDVGVAYDTDIDRATAIINAVGEQLQQDPDWS
                     TRLLEAPSVVRVNALADSAVTLKVLGQVRAAEQWAVAGELRKRILAAFAKEGIEIPFP
                     HRVVVSRPGSSGDQLSDAEAAAASD"
     gene            2960..4255
                     /locus_tag="VFV59_04155"
     CDS             2960..4255
                     /locus_tag="VFV59_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325056.1"
                     /translation="MTANQRRPGFRLPWSSDHEEAEPASEPTSDASAPTVTTESAPAD
                     APVPAEPGSAGTTEALAAATDTNAPATEPAAGTMATGEPIAAAEPATSGVPAPTNGAG
                     PAAGAGEAPEAFLRDLVDAMRGVAEEARDAGLADLRAQADAAVKQLEEVAERRRGELR
                     ERAETDVAAVGEWATAEADRIRREAESRVAARRSQLEEQLAAETRRSEAEAKALRERV
                     AEYEREIEAYHRQLNDIHDPAAFAAAAKRMPKPPALTVTAAPPVARTESTAPPVEPTA
                     AAPAEPATPAAPTVAEPATNGTSTTPTAETTEIHPAEEEVLAGRLAELDAELEPATAA
                     PAEPTSATESGEPMTTDVVVKGLGSFGAITGFRQSLAGVSGIDGVALSLGSSGEFIFR
                     TTHRPSVDVAAAISGLEGDGAKVERRADGTLAVTLDRAR"
     gene            4538..7210
                     /locus_tag="VFV59_04160"
     CDS             4538..7210
                     /locus_tag="VFV59_04160"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU5325057.1"
                     /translation="MTTTRELPPRYDAAAVEPAIYERWMAADAFRPAETPPAGAEPFV
                     IIQPPPNVTGALHLGHAARTTVEDTLIRYHRMRGDDTLWLPGVDHASIAAQFVLDRIL
                     AEEGESRASLGRERYLERMWQFMAETRGVIGTQHRRLGASADWSRERFTMDEGSARAV
                     RVAFKRLWDAGLVYRGEALVNWCPRCLTTISDLENVHRDEAGTLWMIRYHLERPDGLP
                     DPDAWVTVATTRPETLLGDTAVAVHPEDERYAALVGRHAVLPFLGRRLPIVADEHVER
                     GFGTGAVKITPAHDADDYELAKRHGLPAITVLDEHARVNEAGDDFAGMDRYAARKEIL
                     ARLDALGDLVGEQAHQMVVGHCDRCGTVIEPRLSVQWFVRVKPLAERALASVREGRTR
                     IVPSHFEKVYAHWMENIHDWAVGRQLWWGHRIPAWYCPDDHITISDAADGPDACAECG
                     RPASELRQETDIFDTWFSSGLWPFSTLGWPDQTPDLQRFYPTSVMETAYDILFFWVAR
                     MMMLGLFCTDTEPFHTVYLSGMIRDPYGQKMSKTKGNVVDPLETVDEIGADALRFALV
                     NGTTPGADQRLTAAKLDGGRNFTNKLWNAARFVLANRPPRSEPSDGISTSPSLPVRWI
                     RSRLADATERATRQLDALDLGGYAATVYEVAWSDYCDWFLEAVKVDLRREDATDAERA
                     ATWSSAAESLAVLLRLLHPLMPFVTEEIWQALREASPAAAGDEPLLIAAPWPVPPARD
                     REAEAAFDGLATLVRGVRNLRMEAGVPAGTWLPLTIAPADAGASDVLHGGTRYVEALA
                     RVRPIKLDPEAAPPPLVATGPLGAAWLEVQPPSGDVADRRRVRAAELDANIERVRQLL
                     GNASFVERAPAAVVDRERERLRALEAERTQLGDV"
     gene            7308..8036
                     /locus_tag="VFV59_04165"
     CDS             7308..8036
                     /locus_tag="VFV59_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325058.1"
                     /translation="MSFLRRKKQDAAPPPPPTPVHEEITAQEYSLRLSFVARSSDGLR
                     LPADPRVASAIPGIVEPLSQTPVEVIAPLPLEYADASPALERFNEVQQWVLARREVGP
                     IARHGLYVLEMTDALDMTVDTFYCGLLHGDTDTSGYPEYNAIVGGLASHWDELSGELI
                     VRAVLGWGGKGLRGDTDRIGQRLLSGLYQQVLASGYSLGEAEQARLPSIGGRSGLVCA
                     HCGFEAGSAGAFYCPKCGMRMVRG"
     gene            8168..9373
                     /locus_tag="VFV59_04170"
     CDS             8168..9373
                     /locus_tag="VFV59_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5325059.1"
                     /translation="MERPTTLLAVDGEVDSYAEALTRAGFLPLRELAADGSRPDLAVI
                     DCDLPAERVVTIHRTLAEAHTPMLLLVGETTELPSGVGGTNVEVALKPLPGDALVFRL
                     QALLIRSGNHLPSESGAWAGDGLASAPIAGEGHVVSVFAPKGGVGKTTVAVNMAVALR
                     QQTRAEVLLFDADVGVGNVTSVLDVPYRLGLADLADSAPEDWTDTAFDQAVSIHTETG
                     VRVLTWGTDPAESERVTVDLLLAALRWARNHHSYVIIDNHPGYDDRTMAMLAVASEIF
                     LVVTPEVGALRNSSQFLELARELGLGGNVRVIVNRANHGIRLEDMSSSLGLPISATVV
                     SNGPKAVIASNEGRPLVTKFPKEKVADDLHRVARLVSQPNRAVESRAPATAKAKPWWA
                     RLGARTSQA"
     gene            9472..9612
                     /locus_tag="VFV59_04175"
                     /pseudo
     CDS             9472..9612
                     /locus_tag="VFV59_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
     gene            complement(9627..9809)
                     /locus_tag="VFV59_04180"
     CDS             complement(9627..9809)
                     /locus_tag="VFV59_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325060.1"
                     /translation="MGADDDFRAASAPPVDEAADEDPPPSDEDDEEDEDDASALVDDG
                     TEPVTAFELPFLESVE"
     gene            complement(10107..>10508)
                     /locus_tag="VFV59_04185"
     CDS             complement(10107..>10508)
                     /locus_tag="VFV59_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5325061.1"
                     /translation="APRIAPISETILRLPPRQAAPPPPAQPPEQQVAAAPESPEVAAR
                     RAQLDLLGLGDPGEGPVAPERPAVLPYRSRGAAALPPASAGGFWEASAREVAGAIGGQ
                     IGVQSCGQCGLSLSANARFCRRCGARQAQSA"
BASE COUNT         1593 a         3790 c         3715 g         1410 t
ORIGIN      
        1 cttggacccc ccgtgctacc ctagggcgcg atgacgacga agtcgaaagt cccgtatcgc
       61 tgctcgttct gcggcaagag ccaggagcag gtccgcaagc tcatcgccgg acagggcgtc
      121 tacatctgcg acgagtgcat caacctctgc caggagatca tcgacgagga gatgctcgac
      181 gcgccgcgta acggcgcgaa ggcgcagaac acgcccctgc cgagcccgcg caagatcaag
      241 gaccagctcg accagtacgt catcagccaa gaggcggcca agaaggtcct ctccgtggcg
      301 gtctacaacc actacaagcg ggtcaacgcc ggccacaccg tcgacgacgt cgagctcggg
      361 aagagcaacg tcctcctcgt gggacccacc ggctccggca agaccctgct ggcccagacc
      421 ctggccaagg tcctcgacgt tcccttctgc atcgccgatg cgacctccct cacggaagcc
      481 ggctacgtcg gcgaggacgt cgagaacatc ctgctgcgcc tcatccaggc cgccgatttc
      541 gacgttcagc gcgcgcagcg cgggatcatc tacatcgacg agatcgacaa gatcgcgcgc
      601 aagacggaca acccgtccat cacgcgtgac gtctccggcg agggcgtgca gcaggcgctg
      661 ctcaagatcc tcgagggcac cgtcgccaac gtcccaccgc agggcggtcg caagcacccg
      721 caccaggact tcatccagat cgacaccacg aacatcctct tcatctgcgg aggggcgttc
      781 gaggggctgg agaagatcat cgaggagcgg gtcggccggc ggagcatcgg cttcggctcg
      841 cagggcgcca agcagaccaa ggacgagcgt cgccaggcgc tgttcgacaa gctgatgccg
      901 gaggatctgc tgaagtacgg gctcatcccc gagttcgtcg gccgcctccc ggtcgtcgtc
      961 agcctctcgg ccctcgccaa ggaggacctg gtcaaggtcc tgaccgagcc gaagaacgcg
     1021 gtcacccgcc agttcacgaa gttcctgagc ctggacaagg tggagctcgt cttcacgccc
     1081 gatgcactgg aggcgaccgc caagctggcc ctcgaccgca agaccggcgc tcgcgccctg
     1141 cggacgatcg tcgaggaggc gctgcttgac gtcatgtacg acatccccga gcgcacggac
     1201 gtcaagaagg tggtcatcac cgccgagacc atcgagcacg gccagccgcc gatgctggtg
     1261 accgagaccg atgccgagac ggagcctcgg cgacgcggtc gcggccgtcc gtcgcgtgac
     1321 gaggagccgg aggaagagtc ggcctgacgc atcggccggg gggcatggac cgttcgagcg
     1381 ccctccatgc ggcgctgcgc ctctccgtgg agcacctgtg gtggaccaac cggcggctcc
     1441 tggtcgcagc cgcgcggctc cccgatggcg cctgggaggc cgaccacgtg accacccgca
     1501 gcctgcgtgc gacgctcgtc cacgagctcg acgtcgagtg gagctggcgg ctggccctcg
     1561 agggtcgccc ggtggaggag tggggctcgg atgccgagat gcgccccgag gacttcccga
     1621 gcgtcgagtc actccgcgag cggtggagcc gggaagccga cgcgctgcgc cagtgggtgg
     1681 aggggctttc cgaggacgag ctgctggccg agacgaggcc cggactgagc agccgaccgt
     1741 tgccgcgctg gatgttcatc cagcacctgg tgagtcacgg cgcacagcag gccgcggacg
     1801 cggcgacgct cctcacgagc gccggggcgt caccgggcga tatcggcttc ctcgagtaca
     1861 tcggggagag ccaccgcggc cgctgagccg cgctcgtcgg cctagtcgga ggccgccgcg
     1921 gcctcggcgt cgctgagctg gtcgccgctc gaccccggcc gtgacacgac cacgcggtgc
     1981 gggaacggaa tctcgatccc ttccttcgcg aaggcggcca ggatccgctt ccgaagctca
     2041 ccggccacgg cccactgttc cgccgcccgc acctggccga gcaccttcag cgtgacggcc
     2101 gagtcggcca gcgcgttgac gcgcacgacc gatggcgcct cgagcagacg ggtcgaccaa
     2161 tcggggtcct gctgcagctg ctcgccgacg gcgttgatga tggcggtggc gcggtcgatg
     2221 tccgtgtcgt acgcgacgcc gacgtccagg ttcacgcgcg cccacagccg ggtgaggttc
     2281 gaggccaccg agatctggcc gttcggcacc gaatgcaccg ttccgtcgag atcgcgcagc
     2341 gtcgtccggc gcaggctgaa gtcctcgacc acgccgtcga cgccggcgat ccgcaccacg
     2401 tcgccctggc tgtactggtt ctccaggacg atgaagatgc cggcgagcca gtccttgacc
     2461 agcgtctgcg ctccgaggcc gacggcgatg ccgaccacgc cgagacccgc caccgcgggc
     2521 ccgatgtcga cgccgaactc gttgagcacc atcagcaccg cgatcacggc cacgatggcg
     2581 ccggcgacgc gcaccaacag gcgaccgacg gtgttcatcc gccgctcgag ctcgaccggc
     2641 gtgtcactct cgccgccgcc ctccgcgctc cgacgttcca ggaggtggcg caccgccacc
     2701 tgaatcgcga accgcacgcc gacgaacgcg gccagcgcga cgagggcgat caggccgatg
     2761 cgcaggaccg tctcgaccga gtcggggagg tcggggaagg cgggcatggc cggcatggta
     2821 ccaaccgatg cgggggtacc cgggtcccga tcccggccgg tacttccggt cgctgcgaac
     2881 gtgcccaccc gcctcggtag gatgtggaca gcgcgcacca ccgccgctgg cgtcggaacg
     2941 cgcgaggaag ggaacaccga tgaccgccaa ccagcgtcgc cccgggttcc gcctcccctg
     3001 gtcatcggat cacgaggagg ccgagccggc gagcgaaccg acctccgacg cgagcgcgcc
     3061 gaccgtcacc accgagtccg cgccggccga cgcgcctgtg ccggccgagc ccgggtccgc
     3121 tggcaccacc gaggctctcg ccgccgcaac ggacacgaac gcgccggcga ccgagccggc
     3181 agcaggcacc atggcgacgg gcgagccgat cgccgccgcc gaacccgcaa cgtccggggt
     3241 gccggcgccc accaacgggg caggcccggc ggccggagcc ggcgaggcac cggaggcctt
     3301 cctccgcgac ctcgtcgacg ccatgcgcgg cgtggcggag gaggcacgcg acgccggcct
     3361 cgccgacctg cgggcgcagg ccgatgcggc ggtcaaacag ctggaggagg tcgccgagcg
     3421 acgtcgcggc gagctgcgcg agcgtgccga aacggacgtc gccgcggtcg gcgagtgggc
     3481 gaccgcggaa gcggaccgca tccgtcgcga ggccgagtct cgcgtcgcgg ctcgccggag
     3541 ccagctcgag gagcagctgg cggccgagac ccgccgaagc gaggcggagg cgaaggcact
     3601 gcgcgagcgc gttgccgagt acgaacgcga gatcgaggcc taccaccgac agctcaacga
     3661 catccacgat ccggccgcgt tcgcggccgc cgcgaagcgc atgccgaagc ctccggccct
     3721 gaccgtcacc gcggcgccgc cggtggcacg gaccgagtcc acggccccgc cggtcgaacc
     3781 aacggccgcg gcgccggccg agccggcgac accggctgcc ccaaccgtgg ccgagcccgc
     3841 gaccaacggg acctcgacga ccccgacggc cgagacgacc gagatccatc cggccgagga
     3901 ggaggtcctg gcgggccgcc ttgccgagct cgatgccgag ctggagccgg cgaccgcggc
     3961 acccgccgaa ccgacgagcg cgacggaatc cggcgagccg atgaccacgg acgtcgtcgt
     4021 gaagggcctg ggaagcttcg gcgccatcac cgggttccgc cagagcctgg ccggcgtgag
     4081 cggcatcgac ggggtcgcgc tgagcctcgg ctcgagcggc gagttcatct tccgcaccac
     4141 gcatcgaccg agcgtggacg tggccgccgc gatctccggc ctggagggcg acggcgcgaa
     4201 ggtggagcgc cgcgccgatg gcaccctggc ggtgacgctc gaccgcgccc gctgaggtca
     4261 tggagctcgc tggtgccggg tgctagactc ggcgcacggc gaggaccgtg aggagtaccc
     4321 ctccatcggc cggcagagag gtcgggtcgc aggctgagag cccgaccgga tgcgagaggg
     4381 ggaaggtcgc acgcgagccg gacgggtccg cccgatatcg cggatgagag gcggtcccga
     4441 accgggaccg cgaacgaagg tggtaccacg acccgttcgt ccttcgacga gcgggtcgtt
     4501 cgtgtgttcg agcaggaccg atgcaggagg gcgaccgatg acgacgacgc gtgagcttcc
     4561 cccgcgctac gacgccgccg ccgtcgagcc ggcgatctac gagcgctgga tggcggccga
     4621 tgccttccgg ccggcagaaa cccccccggc cggcgccgag ccgttcgtca tcatccagcc
     4681 gccgccgaac gtgaccggcg cgctgcacct cggccatgcc gcgcgcacca cggtcgagga
     4741 cacgctcatc cgctaccacc gcatgcgggg tgacgacacc ctgtggctgc ccggcgtcga
     4801 tcacgccagc atcgccgcgc agttcgtcct ggatcggatc ctggccgagg agggggagag
     4861 ccgggcctcg ctgggccgcg agcgctacct ggagcgcatg tggcagttca tggccgagac
     4921 gcgcggggtc atcggcaccc agcaccggcg gctgggggcc agcgccgatt ggagccggga
     4981 gcgcttcacc atggacgaag gcagcgcccg cgccgtgcgc gtcgcgttca agcggctctg
     5041 ggatgccggc ctcgtctacc gtggcgaggc cctggtcaac tggtgcccac gctgcctgac
     5101 caccatcagc gacctcgaga acgtgcaccg tgacgaggcc ggcacgctct ggatgatccg
     5161 ctaccacctg gagcgcccgg acggcctccc ggaccccgac gcctgggtca cggtggccac
     5221 cacgcggccg gagacgctgc tcggcgacac ggcggtcgcc gtccatcccg aggacgagcg
     5281 ctacgccgcg ctggtcggcc gccacgcggt cctgccgttc ctcggccgtc gcctgccgat
     5341 cgtggccgac gagcacgtcg agcgcggatt cggaacgggc gccgtgaaga tcacgccggc
     5401 gcacgatgcc gacgactacg agctcgccaa gcgccacggg ctgccggcca tcaccgtcct
     5461 ggacgagcac gcgcgggtga acgaagccgg cgacgacttc gccggcatgg atcggtacgc
     5521 ggcgcggaag gagatcctcg cgcggctgga cgccttgggc gacctggtgg gggagcaggc
     5581 gcaccagatg gtcgtcgggc actgcgaccg gtgcggcacg gtcatcgagc cgcggctgtc
     5641 ggtgcagtgg ttcgtgcgcg tcaagccgct ggcggagcgg gcgctcgcat cggtcaggga
     5701 gggccggacg cggatcgtgc cgtcgcactt cgagaaggtg tacgcgcact ggatggagaa
     5761 catccacgac tgggcggtcg ggcgacagct ctggtggggc caccggatcc cggcgtggta
     5821 ctgccccgac gaccacatca ccatcagcga cgcggccgac gggccggacg cgtgcgccga
     5881 gtgcgggcgg ccggcatcgg agctgcggca ggagaccgac atcttcgaca cctggttcag
     5941 ctcggggctg tggccgttct cgaccctcgg ctggccggat cagaccccgg acctgcagcg
     6001 cttctacccg accagcgtca tggagaccgc gtacgacatc ctcttcttct gggtggcgcg
     6061 gatgatgatg ctcggcctct tctgcaccga taccgagccg ttccacacgg tctacctctc
     6121 gggcatgatc cgggacccgt acggccagaa gatgagtaag accaagggca acgtcgtcga
     6181 cccgctcgag acggtggacg agatcggggc cgatgcgctg cggttcgcgc tcgtcaacgg
     6241 caccacgccc ggtgccgacc agcgcctgac cgccgcgaag ctcgacggcg ggcgcaactt
     6301 caccaacaag ctgtggaacg ccgcgcgctt cgtcctggcc aaccgtccgc cccggtcgga
     6361 gccgtccgac gggatctcga cctccccgag cctgccggtg cgctggatcc ggtcgcgcct
     6421 ggccgacgcg acggagcgcg ccacgcgcca gctggacgcc ctggacctgg gtggctacgc
     6481 cgcgacggtc tacgaggtgg cgtggtcgga ctactgcgat tggttcctgg aggcggtcaa
     6541 ggtggatctg cgccgcgagg acgcgaccga cgccgagcgc gcggcgacgt ggtcctcggc
     6601 cgccgagtcg ctcgccgtcc tcctgcgtct cctccatccg ctgatgccgt tcgtcaccga
     6661 ggagatctgg caggcgctgc gggaggcatc gcccgccgcc gcgggtgacg agccactgct
     6721 catcgcggcg ccgtggcccg tccctccggc gcgcgaccgc gaggccgagg cggccttcga
     6781 cggcctcgcg acgctggtgc gcggcgtccg gaacctgcgc atggaggccg gggtcccggc
     6841 cgggacctgg ctgccgctca ccatcgcgcc ggccgatgcc ggcgccagtg acgtcctcca
     6901 cggcggcacc cggtacgtgg aggccctggc gcgtgtccgg ccgatcaagc tcgaccccga
     6961 ggcagcgccg cccccgctgg tcgcgaccgg accgctcggc gccgcatggc tcgaggtcca
     7021 gccgccatcc ggcgacgtcg cggaccgtcg ccgcgtgcgg gcagcggagc tcgacgcgaa
     7081 catcgagcgc gtccgccagc tgctgggcaa cgcgtcgttc gtcgaacgcg ccccggcggc
     7141 cgtcgtcgat cgcgagcgcg agcggctgcg cgccctcgag gcggagcgca cgcagctggg
     7201 cgacgtgtga gcggtctcag cggatgcccc caatgggcca tggcgggcga ccgctgggcc
     7261 gctggtagaa tcggccgcgc tgtcaccacc gagaggccaa cgccccgatg tccttcctga
     7321 ggcgcaagaa gcaggacgcg gctccgccgc cgcccccgac cccggtccac gaggagatca
     7381 ccgcgcagga gtactcgctg cgcctctcgt tcgtggcgcg gtcgagcgac gggctgcgac
     7441 tccccgcgga tcctcgcgtc gcctccgcca tcccgggcat cgtcgagccg ctgagccaga
     7501 cgccggtcga agtcatcgcc ccgctgcccc tggagtacgc cgacgcctcg ccggcgctgg
     7561 agcgattcaa cgaggtgcag cagtgggtcc tagcgcggcg cgaggtcggg ccgatcgccc
     7621 gccacgggct gtacgtgctg gagatgaccg acgccctcga catgacggtc gacaccttct
     7681 actgcggcct gctgcacggc gacaccgata ccagcggcta cccggagtac aacgccatcg
     7741 tcggcggcct cgcctcgcac tgggacgagc tctccggtga gctcatcgtc cgggcggtgc
     7801 tcgggtgggg cggcaaggga ctgcgtggcg acaccgaccg catcggtcag cggttgctct
     7861 cgggcctgta ccagcaggtg ctggcgtcgg gatacagcct gggcgaggcc gagcaggcgc
     7921 gcctcccctc catcggcggc cgatccggcc tggtgtgcgc gcactgcggc ttcgaggcag
     7981 ggagcgccgg ggcgttctat tgcccgaagt gcggcatgcg catggtccgc ggctgaggcg
     8041 ctccgagccg ggcagtactt tcgggcgggg gacgctgggc cgcagtccca ttgccgctcg
     8101 ccgtggtcgt cgggagactg gtgcagtccg tcaccgtacc tccgtactcc accgctgaag
     8161 gaaggccatg gagcgaccta ccaccctgct cgccgtcgac ggcgaagtcg actcgtatgc
     8221 cgaggccctg acccgcgccg gcttcctgcc gctgcgtgag ctcgcggccg acgggtcgcg
     8281 ccccgacctc gccgtcatcg actgcgacct gcccgcggag cgggtggtga ccatccatcg
     8341 cacgctcgcc gaagcccaca cgccgatgct gctgctggtc ggcgagacca ccgagctgcc
     8401 atccggcgtc ggcggcacga acgtggaggt ggcgctgaag ccgctgcccg gcgatgcgct
     8461 cgtcttccgg ctccaggcgc tgctgatccg ctccggcaac catctaccgt ccgagtcggg
     8521 cgcgtgggcg ggcgacggcc tggcgtcggc gccgatcgcc ggcgagggcc acgtcgtcag
     8581 cgtcttcgcc ccgaagggcg gcgtcggcaa gacgaccgtg gcggtcaaca tggccgtcgc
     8641 gctccgtcag cagacccgcg ccgaggtcct gctcttcgac gccgacgtcg gcgtcggcaa
     8701 cgtgacctcc gtgctcgacg tgccgtatcg gctggggctc gcggacctcg cggacagtgc
     8761 gccggaggac tggaccgata cggccttcga ccaggccgtg agcatccata ccgagaccgg
     8821 cgtccgcgtc ctgacgtggg ggaccgatcc agcggagtcc gagcgggtca cggtcgacct
     8881 cctgctcgcc gcgctgcgtt gggcgcggaa ccaccactcc tacgtgatca tcgacaacca
     8941 tccggggtac gacgaccgca ccatggcgat gctcgcggtg gccagcgaga tcttcctcgt
     9001 cgtcacgccc gaggtcggtg cgcttcgcaa cagctcgcag ttcctggagc tggcccgcga
     9061 gctcggtctc ggcggcaacg tgcgcgtcat cgtgaaccgc gccaaccacg gcatccgcct
     9121 ggaggacatg tcgagctcgc tgggcctgcc catctccgcc acggtggtga gcaacgggcc
     9181 caaggcggtc atcgcctcga acgagggtcg gccgctggtc accaagttcc cgaaggagaa
     9241 ggtggcggac gaccttcatc gggtcgctcg tctcgtctcg cagccgaacc gcgccgtcga
     9301 gtccagggca ccggcgacag ccaaggccaa gccatggtgg gcgcggctcg gggcgcgcac
     9361 ctcacaggcc tgaaccgcca tcggcgaacc gcgcgccgcg cggtgatcgt cttccggccg
     9421 gcgggggccc gcgttgcgcg gcaccgccgg ctttgccatg atccgtgagc cgtgccgacc
     9481 tacgactacc agtgccgctc ctgcggcacg atcaccgaag tcatccactc catgctcgag
     9541 gacgggccgt ccgtctgcga gcgctgcggt ggtgagctgc gtcgcgtcct ctatccggcc
     9601 ggcatcatct tcaaggggcc cggcttctac tcgaccgact cgaggaacgg cagctcgaag
     9661 gccgtgaccg gttcagtgcc atcgtccacg agcgccgagg cgtcgtcctc gtcctcctcg
     9721 tcgtcctcgt ccgatggcgg agggtcctcg tcggccgctt cgtcgacggg tggagccgat
     9781 gcggcacgga agtcgtcgtc ggcgcccaag ggcgacgccg cctgaccatc gctcggcggc
     9841 gggccgaaac ccattgaagt tcccgccgct cgggcggctg atcggttggc cggcgatcgc
     9901 ggtcgtcaga ttcccggctg ccgggcagga agagccccgc tttcccgctc tttcggtacc
     9961 tgacgacccg gctcgccttg tgcctacgtc gctgcccgcc attcgggaat gtggctggct
    10021 cgccaccctt cggcgtcgcc gcgtgctcca acgtcgggaa tcgtggcggt ctggaagtcg
    10081 ggtcgagtgc tgtcccgagt tgcgactcag gccgattgcg cctgacgcgc gccgcaccgc
    10141 cggcagaatc gggcgttcgc cgagagcgac aggccgcact gcccgcagct ctggacgccg
    10201 atctggccac cgatggcgcc ggccacctcg cgggccgatg cctcccagaa ccctccagcc
    10261 gaggccggcg ggagcgcggc ggcgccgcgt gagcgatagg ggagcaccgc cggccgctct
    10321 ggtgcgaccg gcccctcgcc ggggtcaccg aggccaagga gatccagctg cgcgcgacgg
    10381 gccgcgacct cgggcgactc cggcgctgcc gcaacctgct gctccggggg ttgagcgggt
    10441 ggcggtggtg ctgcctggcg tggaggcagg cggaggatcg tctcgctgat cggcgcgatc
    10501 cgcggcgc
//
LOCUS       DASTYP010000087        11417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1682197, whole genome shotgun sequence.
ACCESSION   DASTYP010000087 DASTYP010000000
VERSION     DASTYP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682197"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..612
                     /locus_tag="VFV59_04190"
     CDS             79..612
                     /locus_tag="VFV59_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5325062.1"
                     /translation="MINAPVLVLNQNYEPLNVCDIRRAFRLLGASKAELLARNHQVIR
                     TPTSAIPAPSVIRLQYHVKRPQPRVRLSRREVFSRDRHTCQYCGVVSRELTIDHVVPK
                     HRGGRHEWENLVAACRPCNHRKGGKTVAEARMHLLREPRAPRADLRYLFGTYLADDRN
                     DAWRNYLFLGDPAPNPS"
     gene            564..2015
                     /locus_tag="VFV59_04195"
     CDS             564..2015
                     /locus_tag="VFV59_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU5325063.1"
                     /translation="MAQLPVPGRPGAEPVLIDGAAFAIPDLPDAPRTALGALTAAGHE
                     AVLVGGSLRDLLRGERPEDWDIATSARPEEVSALFPGSSWENRFGTVTLPGADHVEIT
                     TYRSESGYADRRRPDEVVFHRSLHDDLARRDVTVNAVAWLPDAPGATTGRLVDPHGGL
                     EDLRTGRIRTVGDPADRFAEDALRVLRTVRFALRFGFAIDPATDEGLRAAAPSTASLS
                     AERVRDELRRLLADPGIRPSVAFGRWEGLGILPVLLPELAALRGIPQGKPIPGDALDH
                     SLRTADALPAHDPILRLAGLLHDLGKATTLSDGHFIGHEVVGAGMAEGVMRRLRFAAP
                     EVARVREVVRHHMFAYEPAWTDAAVRRFIKRVGPDRLADLFALRVADDVASGADEPAV
                     GGLDELRRRVEEQREAPMSSWHLAIDGHDLQREIRMAPGPPMGRLLDRLVEAVLDDPA
                     LNRRDVLLEMARAWVTGGDTVHHRDAGEPGPEG"
     gene            2111..2806
                     /gene="deoC"
                     /locus_tag="VFV59_04200"
     CDS             2111..2806
                     /gene="deoC"
                     /locus_tag="VFV59_04200"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5325064.1"
                     /translation="MASSVRRAVRLEHRLTRPNASLADLDAAVALATEHELSGLTVSP
                     WLVKAAKRALGRSRVTLGTVVGFPHGGQLLSVKAFEASKALEQGAGQIDFVLNGGALV
                     SGDEETVFNDMLAVVDMAHSALAAAGVIVEPEAMGEDLLRQACRLAERAGADYVVTGT
                     GTSSAAAAVQRTRLLRDSVGPRIGVKAAGRFRSADQLREALEAGATRVTTVLSGALAR
                     QATAEPTPAGAAS"
     gene            2819..3859
                     /gene="galE"
                     /locus_tag="VFV59_04205"
     CDS             2819..3859
                     /gene="galE"
                     /locus_tag="VFV59_04205"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU5325065.1"
                     /translation="MKILVVGGAGYVGSTSVERFVDAGHEVVVYDNLTSGHAAAVPDG
                     VRLVVGDIGDRVRLEALLGDGVDAVLHCAARSLVGESMSDPGLYYRTNVAGGVTLLDA
                     MNATGVTRLVYSSSAAVYGEPRRVPIAEADRTQPINPYGETKLAFEGAMRWFAAAHDF
                     RAISLRYFNVAGATERNGEDHDPETHLIPLVLRVAAGEASHVQIFGQDYPTPDGTCIR
                     DFVHVRDLAEAHLLALEATADSEPSLEIYNLGSAAGFSVREVVEAARKVTGRAIPARV
                     LKRRSGDPPVLVASSRRARRELGWQPRHSTLEQMLADAWTWRQAHPHGYADGQATGRS
                     VTSEDRVAASAR"
     gene            complement(3848..4291)
                     /locus_tag="VFV59_04210"
     CDS             complement(3848..4291)
                     /locus_tag="VFV59_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU5325066.1"
                     /translation="MSSSSVAERLHESGERVTRQRLLIADALEASGRQLSAQELYEQL
                     RRRHRGIGRATVFRTLEALVTAGVARRLEQGGNAHLFVACAPSHHHHLACTRCGRVEE
                     IDEGYVRPVADRLAADLGFRIDDARLDFYGLCAACAAAEERPSAG"
     gene            complement(4288..5040)
                     /locus_tag="VFV59_04215"
     CDS             complement(4288..5040)
                     /locus_tag="VFV59_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEU5325067.1"
                     /translation="MPLAAALVSASAFISALAGGFLALRAVAHVGPLIAFGAGIRIGA
                     AYFDLIPESVEHLGGSMDAAMIATAIGFLAFYAIEKLTTIHVGHEAASELDHGGIGHR
                     HIGFVGAAGMSFHSLLDGVALAAGLTVGGPLALVIAVVVVVHRFSDGIGIVSFLIASH
                     TSPALIYRWVALVAVAPVVGVVFGLLIPVPEVVLGAMLGVFAGFFLYIGAAELLPEAH
                     RSDRSSRVVLATLAGVVAIYLLSVFAGAVGAH"
     gene            5159..6430
                     /locus_tag="VFV59_04220"
     CDS             5159..6430
                     /locus_tag="VFV59_04220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5325068.1"
                     /translation="MPDPAPSRPRPRRRPSRGATEAHGSSPASTETLDAPGDLEAAVI
                     AAASAYRFGDIEVPATIARSLERMGYRTPTEVQARAIPPLRRGDDLIGQAQTGTGKTA
                     AFGIPIIEKTDPSADHVQALVLTPTRELCVQVADEVARIGQFRGVRTVAIYGGSSMDR
                     QIEALRRGTPVVVGTPGRVLDLIRRGELRLDRVHTVILDEADRMLDMGFIVDVETILS
                     RCPRQRQTALFSATMPYAILRICDRFMKRDATHVTVRPEMKTVETVRQLVFFVAEQDK
                     VAALVELAERFGFDRLLVFRHTQIGVDRLAATLARRGKNVAALHGGLSQRERDRTLAA
                     FKAGKIQFLVATNVAARGLDITDLPYVLSFDIPEDADTYVHRIGRTGRAGREGTAITF
                     VGEWDLDAWEAIKAEVGAEVEEGELSLYDAG"
     gene            6491..7174
                     /locus_tag="VFV59_04225"
     CDS             6491..7174
                     /locus_tag="VFV59_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325069.1"
                     /translation="MGPPIFPQRPTEARAEVLSVEWLFEPMWPGERLIARCRGGVVQL
                     SDELARPADDRFPEVRQLLATAVLSDDAVVDGVWTAQPFVGDGSPARAWAETLEAEGL
                     ADEMPDPLELERRRAFVAVDLLEIDGQDLTDVPFQERRRLLESVIDEGVQARLSPLVK
                     LPVAGWLTAWRANGFTHYLAKHQNSRYHPGERNDDWLKLSTRVEAPKGMVGRIVGFGR
                     GERIRRIRD"
     gene            7396..8769
                     /locus_tag="VFV59_04230"
     CDS             7396..8769
                     /locus_tag="VFV59_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU5325070.1"
                     /translation="MRPERTRRCEPARRLVLTSDPPTTAPAASALDDDALAEVRRVGT
                     ADLMVGIPSFSNAETIGYVVRAATAGMVQYFPELKPILVNSDGGSPDDTPRVVVSTES
                     PDYLEQVILVSPKHKLQRVSFTYQGTSGKGTAVRALFEVARELKVKAMVMVDSDLRSI
                     VPEWVELLAGPILKGGYDFVAPMYARYKYDGTITNNISYPLTRALYGARIRQPIGGDF
                     AVSGDLVQRYLELDTFDELTARFGIDIWMTHSAINEGFAVCQARLGAKIHDAKDPASD
                     LGPMFRQVVGTLFNLAGHYEDRWLRVRGSHPIPEYGFERVISPEEIAVNHAKLIDNLE
                     GARLTQRDVWSEMLAPEQLERVMTIDYGGEPADFHFSAELWVRCLYDALVAFHRPGTD
                     RERLLAALTPLYFGRTAGFISDTLEMTTDQAERVIEEQARTFEELKPYLVARWREMRA
                     LAEQRTA"
     gene            8769..10751
                     /locus_tag="VFV59_04235"
     CDS             8769..10751
                     /locus_tag="VFV59_04235"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEU5325071.1"
                     /translation="MAAARRKAPYRILLPLANPRTARDLVRIGAGIANGRPTEITALG
                     IVEVPEGVSLSEGATHARTQRRLLQRVLDFGDEEGVELRTLVRIGRHAADGVIEAVGE
                     ESADLVIFGWGGPPTPAQLARAEAAEAGEPSPALEPPAPRPVFSPTIDAVVRESPCDI
                     AVVKQRGLEHVESILVPVRGGPHAELAMRISRDLAKRFGAKIVVLHVVPKGVGERAVE
                     REQAALDAFVREHAGTRRATGLIREASSVRQAIIKEAAHHGLVVMGASAHPTNASPDG
                     RYLFGTLAEAVASKAKPTVMVVKTKQSLGLATFEELRASEGTLAHADAFVERSRSLPV
                     IVDKWFAENTFHAHEFADIRKLLALKERHGVTISVALPALNEEKTIGTVIKRVKGALM
                     DRVPLIDQLVVIDSDSEDRTVDIAGELGVPVFRHPEILPETGSYRGKGEALWKSLHVL
                     DGDIVAWIDTDISNIQPRFVYGLLGPLLREPRIQYVKGFYQRPIREGDKLQAEGGGRV
                     TELMARPLINLFFPELSGIIQPLSGEYAGRRALLEQLPFFTGYAVEIGLLIDILEAAG
                     LSAIGQVDLERRIHRNQPLGNLSQMSYVILQGAIRKLEERHRLELLTEVGRGMKLIHT
                     GKENFSLEVREIGDDLRPPIVTVPAYAERRRELKSR"
     gene            10840..>11417
                     /locus_tag="VFV59_04240"
     CDS             10840..>11417
                     /locus_tag="VFV59_04240"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU5325072.1"
                     /translation="MTSSPVAPTAPSPDARAPRPIIRVADELGLLPDEVELHGRLLAK
                     VSLGALSRLADAPLGRLVLVTAMTPTPFGEGKTTTAIGLADALAALGLRSIVTLRQPT
                     LGPVFGVKGGAAGAGRASLVPRQEVNLHLTGDAYAVGAAHNLAAAVLDAHLHHGNVLG
                     IDPTTITWPRVTDHNDRALRHARIAIGEVGER"
BASE COUNT         1650 a         4169 c         4056 g         1542 t
ORIGIN      
        1 ggcgcatcgg tccggctccc ttgcgccgat cgggaaccac gctcgccagt tgctatcatc
       61 gcgactgagg cacgcccgat gatcaacgct ccggtgctgg tcctgaacca gaactacgaa
      121 ccgctcaacg tctgcgacat caggcgcgcc ttccgcctgc tcggggcctc caaggcggag
      181 ctgctggcgc ggaaccacca ggtcatccgc acgccgacct cggccattcc cgcgccgtcg
      241 gtcatccgtc tccagtacca cgtcaagcgg ccgcagccgc gggtgcgtct gagccgtcgc
      301 gaggtgttca gccgcgaccg gcacacctgc cagtactgcg gcgtggtcag tcgcgagctg
      361 accatcgacc acgtggtgcc gaagcaccgt ggcggccgcc acgagtggga gaacctggtc
      421 gccgcctgcc ggccgtgcaa ccaccgcaag ggtggcaaga cggtcgccga ggcgcgcatg
      481 cacctcctgc gcgagccgcg cgcaccgcgg gccgacctgc gctacctgtt cggcacctac
      541 ctggccgacg atcggaacga cgcgtggcgc aactacctgt tcctgggcga cccggcgccg
      601 aacccgtcct gatcgacggg gccgccttcg ccatccccga cctgcccgac gcgccgcgca
      661 cggcgctcgg cgcgctgacg gcagcgggcc acgaggcggt gctggtgggc ggatcgctgc
      721 gcgacctcct gcgcggcgag cggcccgagg actgggacat cgcgacctct gcccggccgg
      781 aggaggtgag cgccctcttt cccggctcga gctgggagaa ccgcttcggc acggtgacgc
      841 tgcccggcgc cgaccacgtc gagatcacga cctaccgcag cgagtccggc tacgccgacc
      901 ggcgtcgacc cgacgaggtc gtcttccatc gctcgctgca cgacgacctc gcgcgacggg
      961 acgtcaccgt caacgcggtc gcctggctac cggatgcccc cggggccacg accggccggc
     1021 tggtggatcc gcacggcggg ctggaggacc tgcggaccgg tcgcatccgc accgtgggcg
     1081 atccggcgga ccggttcgcc gaggacgcgc tgcgcgtgct gcgcacggtg cgcttcgccc
     1141 tgcgcttcgg cttcgccatc gacccggcga ccgacgaggg cctgcgcgcc gcggccccga
     1201 gcaccgcctc gctgtcggcc gaacgcgtgc gggacgagct gcgccggctc ctggccgatc
     1261 ccggcatccg gccgagcgtc gcgttcgggc gctgggaggg cctcgggatc ctcccggtcc
     1321 tcctccctga gctggctgcg ctgcgcggca ttccgcaggg caagccgatc cccggcgatg
     1381 cgctcgacca ctcgctgcgg acggccgatg cgctgccggc ccatgacccg atcctgcgcc
     1441 tggcgggcct gctccacgac ctcggcaagg cgaccacgct gtccgacggc cacttcatcg
     1501 gtcacgaggt ggtcggcgcg ggtatggcgg agggggtgat gcgacggctg cgcttcgccg
     1561 cgccggaggt ggctcgcgtc cgcgaggtgg tgcggcacca catgttcgcc tacgagccgg
     1621 cctggaccga tgccgccgtg cgccgcttca tcaagcgcgt cggcccggat cggctggcgg
     1681 acctcttcgc gctgcgcgtc gccgatgacg tcgcctccgg cgccgacgag ccggcggtcg
     1741 gcgggctgga cgagctccgt cgtcgggtcg aggagcagcg cgaggcgccg atgtcgtcgt
     1801 ggcacctcgc catcgatggc cacgacctgc agcgcgagat ccggatggcc cccgggccgc
     1861 cgatgggccg cctgctggac cggctggtcg aggccgtcct cgacgacccg gcgctcaacc
     1921 ggcgcgacgt gctgctggag atggcgcggg cgtgggtgac cggcggtgac accgtgcacc
     1981 accgggacgc tggggaaccg ggcccggagg gctgacagcc gaggtcgcgc tgctacaatc
     2041 ggccgtccag gcccttctcc caggcactca tctcgcacat cacgcaccga gcggagggaa
     2101 cgcgctttcc atggcctcca gcgtgcgtcg cgccgttcgc ctcgagcacc ggctcacccg
     2161 gccgaacgca tcgctcgctg acctcgacgc cgccgtggcg ctggccaccg agcacgagct
     2221 ttcggggctg acggtcagcc cctggctggt caaggctgcc aagcgagcgc tcggccgctc
     2281 gcgagtgacg ctgggcaccg tcgtgggctt cccgcacggc gggcagctgc tgagcgtgaa
     2341 ggcgttcgag gcatcgaagg cgctggagca gggcgccggc cagatcgact tcgtcctgaa
     2401 cggcggcgcg ctggtgtccg gcgacgagga gacggtcttc aacgacatgc tcgccgtggt
     2461 cgacatggcg cactcggccc tcgcggcggc cggtgtcatc gtggagcccg aggcgatggg
     2521 cgaggacctg ctgcgtcagg cgtgccgcct ggcggagcgc gccggtgccg attacgtcgt
     2581 gaccggcacg gggacatcct ccgcggcggc cgccgtgcag cgcacccggc tgctgcgtga
     2641 cagcgtcggc ccgcgcatcg gcgtcaaggc cgccggccgc ttccggagcg cggaccagct
     2701 gcgcgaggcc ctggaggctg gcgccacacg ggtcacgacc gtcctcagcg gcgcgctggc
     2761 gcgccaggcg accgccgagc cgacgcccgc cggcgcggcg agctgaggga gggatggatt
     2821 gaagatcctc gtggtcggtg gtgccgggta cgtcggctcg acgagcgtgg agcgcttcgt
     2881 cgatgccggc cacgaggtcg tcgtgtacga caacctgacc agcggccatg ccgccgccgt
     2941 tccggacggc gtccggctgg tggtcggcga catcggcgac cgcgtccgcc tcgaggcgct
     3001 cctgggcgat ggggtggacg cggtgctgca ttgcgccgcg cggtcgctgg tcggcgagtc
     3061 gatgagcgac ccgggcctgt actaccgcac caacgtggcg ggcggcgtca ccctgctcga
     3121 cgccatgaat gccaccggcg tcacgcgact ggtctacagc tcgagcgccg cggtctatgg
     3181 agagccgcgc cgcgtcccca tcgccgaggc ggaccggacc cagcccatca acccctacgg
     3241 ggagacgaag ctggccttcg agggcgccat gcgctggttc gccgccgcgc acgacttccg
     3301 cgccatcagc ctgcgctact tcaacgtcgc cggtgccacc gagcgcaacg gcgaggacca
     3361 cgaccccgag acgcacctca tccccctggt cctgcgcgtg gcggccgggg aggcgagcca
     3421 cgtccagatc ttcgggcagg actatccgac gcccgacggc acctgcatcc gcgacttcgt
     3481 ccacgtccgc gacctggccg aggcgcacct gctggccctg gaggccaccg ccgactcgga
     3541 gccgtcgctc gagatctaca acctgggctc ggccgccggc ttcagcgtcc gggaggtggt
     3601 cgaggccgcc cgcaaagtga ccggccgcgc catcccggcc cgcgtcctga agcgccgcag
     3661 tggggacccg ccggtcctgg tcgcgtcgtc gcggcgggcg cgccgcgaac tcggctggca
     3721 gccgagacac tcgacgctgg agcagatgct cgccgacgcc tggacctggc gccaggccca
     3781 cccgcacggc tacgccgacg gccaggccac cggccgcagc gtcacctccg aggaccgggt
     3841 cgcggcctca gcccgctgaa gggcgttcct cggcggcggc gcacgcggcg cacaggccgt
     3901 agaagtccag gcgcgcgtca tcgatccgga agccgaggtc ggccgccagc cggtcggcca
     3961 cgggccgcac gtagccctcg tcgatttcct cgacccgtcc gcagcgcgtg caggcgaggt
     4021 ggtggtggtg gctcggcgca caggccacga acaggtgggc gttgccgccc tgctccagcc
     4081 gccgcgcgac gccggccgtc accagggcct cgagggtgcg gaacacggtg gcccgtccga
     4141 tgccgcggtg gcgccgccgc agctgttcgt acagctcctg cgccgagagc tgccggccgc
     4201 tggcttcgag ggcgtcggcg atcagcagtc gctggcgcgt gacgcgctcg cccgactcgt
     4261 gcagccgctc ggccaccgac gacgagctca atgggcaccc accgcgccgg cgaagacgga
     4321 gagcaggtag atcgcgacca cgccggccag ggtcgccagc acgacgcggc tggaacggtc
     4381 gctgcggtgg gcctccggca gcagctcggc ggcaccgatg tacaggaaga agcccgcgaa
     4441 gactccgagc atcgccccga ggacgacctc cgggacgggg atcaggagcc cgaagaccac
     4501 gccgaccacc ggggcgacgg ccaccagcgc gacccagcgg tagatgaggg ccggcgacgt
     4561 gtggctggcg atcaggaagc tgacgatgcc gatgccgtcg ctgaagcggt gcaccacgac
     4621 cacgaccgcg atcaccagtg ccagcgggcc gccgacggtg aggcccgccg cgagggcgac
     4681 gccgtcgagc aggctgtgga agctcatgcc cgccgcgccg acgaagccga tgtggcggtg
     4741 cccgatcccg ccgtggtcga gctcgctggc cgcctcgtgg cccacgtgga tggtggtcag
     4801 cttctcgatg gcgtagaacg ccaggaagcc gatggccgtc gcgatcatcg ccgcgtccat
     4861 cgagccgccg aggtgctcca cgctctcggg gatcaggtcg aagtacgcgg cgccgatgcg
     4921 gatcccggcc ccgaacgcga tcagcgggcc gacgtgcgcg accgcgcgca acgccaggaa
     4981 gccgcccgcc agggcggaga tgaacgcgct cgcggagacc agcgcggcgg ccagcggcat
     5041 cggtcgggtg ttccttcgct acagacgaga cagagtctca tcttcgcacc cgtgtcaagc
     5101 cggcgtggta cgatcgaacg ccccaccccc gcttgtggtg tccctcctga ggtcccgttt
     5161 gcccgatccc gctccttctc gcccacgtcc ccgccgccgc ccatcgcgcg gcgcgacgga
     5221 ggcccacggc tccagccctg cctcgaccga gacgctcgac gctcccggcg acctggaggc
     5281 cgcggtcatc gcggcggcat cggcctatcg attcggcgac atcgaggttc ccgccaccat
     5341 cgcccggtcg ctggagcgca tgggctaccg caccccgacc gaggtccagg cgcgcgccat
     5401 tcctcccctg cgacgcggtg acgacctcat cggccaggcc cagaccggca ccggcaagac
     5461 ggccgccttc ggcatcccga tcatcgagaa gaccgatccg tccgccgacc acgtccaggc
     5521 gctggtcctg acgccgacgc gcgagctgtg cgtgcaggtg gccgacgagg tggcgcgcat
     5581 cggccagttc cgcggcgtcc gcaccgtggc catctacggc ggctcgtcca tggaccgcca
     5641 gatcgaggcg ctgcgacgcg gcacgccggt cgtcgtcggc acgccggggc gcgtcctgga
     5701 cttgatccgg cgcggcgagc tgcggctcga ccgcgttcac accgtgatcc tcgacgaggc
     5761 ggaccgcatg ctcgacatgg ggttcatcgt cgacgtcgag acgatcctgt cgcgctgccc
     5821 ccgccagcgg cagaccgcgc tgttcagcgc caccatgccg tacgccatcc tgcgcatctg
     5881 cgaccgattc atgaagcgcg acgccaccca cgtcaccgtc agaccggaga tgaagacggt
     5941 cgagaccgtc cgccagctgg tcttcttcgt ggccgagcag gacaaggtcg cggcgctcgt
     6001 cgagctggcc gagcggttcg ggttcgatcg gctgctcgtc ttccgccaca cccagatcgg
     6061 ggtggaccgg ctggccgcca cgctggcgcg acgcggcaag aacgtcgcgg cgctgcacgg
     6121 tggcctctcg cagcgggagc gcgaccgcac cctcgccgcc ttcaaggcgg ggaagatcca
     6181 gttcctggtc gccaccaacg tcgccgcgcg cggcctcgac atcaccgacc tgccgtacgt
     6241 gctgtcgttc gacatccccg aggacgcgga cacctacgtg caccgcatcg gccgcaccgg
     6301 ccgggcgggg cgtgagggca cggccatcac cttcgtcggc gaatgggacc tcgacgcctg
     6361 ggaggcgatc aaggccgagg tcggcgccga ggtcgaggag ggcgagctca gcctctacga
     6421 cgcgggctga cgctggatcg gacgccgccg gcggtgaacc ccacgggtcg gccggcggta
     6481 cgctaggcgg atgggaccgc cgatcttccc gcagcgtccc acggaggcgc gcgccgaggt
     6541 cctcagcgtg gagtggctgt tcgagcccat gtggcccggt gagcggctca tcgcccgctg
     6601 ccgtggtggc gtggtccagc tgagcgacga gctggcgcga ccagccgacg accgcttccc
     6661 ggaggtcagg caactgctgg ccacggccgt tctctccgac gacgccgtgg tcgacggggt
     6721 gtggacggcc cagccgttcg tgggtgacgg cagcccggcg cgcgcctggg ccgagaccct
     6781 cgaggcggag ggactcgccg acgagatgcc ggatcccctg gagctggagc gtcgccgcgc
     6841 cttcgtggcg gtcgacctgc tcgagatcga cggccaggac ctgaccgacg tgccgttcca
     6901 ggagcgacgc cggctgctcg aatcggtgat cgacgaggga gtccaggccc ggctcagccc
     6961 gctggtgaag ctgcccgtcg ccggctggct gaccgcgtgg cgcgccaacg gcttcacgca
     7021 ctacctggcc aagcaccaga actcgcggta ccaccccggc gagcgcaacg acgactggct
     7081 gaagctctcg acccgcgtcg aggcgccgaa gggcatggtc gggcggatcg tgggcttcgg
     7141 gcggggcgag cggattcgcc gaatccggga ctgacgccac ggcgtcacga cggatcgcga
     7201 ccgtttcgca cagcggaccg ctccggaacg cccctcgcga tcgatcgtga tcggggcgct
     7261 ggaggcgtgg cgaacggtca ccctcgcgct cccggagccg cgatgtatcg ccattgcggc
     7321 gcgcgtcgcg gggctccggc gcgttggtcg cgatccgtcg cgacctgcgg tccatcggcc
     7381 ttcgcgtgct accctttgcg ccccgagcgg acacgccgat gtgaaccagc gaggaggctc
     7441 gtcctgacgt ccgacccccc gaccaccgcc ccggccgcgt ccgccctgga cgacgacgcg
     7501 ctcgccgagg tccggcgcgt cgggacggcc gacctgatgg tcggcatccc gtcgttcagc
     7561 aacgccgaga cgatcggcta cgtggtccgc gccgcgacgg ccggcatggt ccagtacttc
     7621 ccggagctca agccgatcct ggtgaactcc gacggcggct cgcccgacga tacgccgcgc
     7681 gtcgtggtca gcaccgagag ccccgactac ctcgagcagg tgatcctggt cagccccaag
     7741 cacaagctgc agcgggtgag cttcacctac cagggcacca gcgggaaggg caccgcggtg
     7801 cgggcgctgt tcgaggtggc gcgcgagctc aaggtgaagg ccatggtcat ggtcgactcg
     7861 gacctgcggt cgatcgtgcc ggagtgggtc gagctgctgg cgggaccgat cctcaagggt
     7921 ggctacgact tcgtggcgcc gatgtatgcg cgctacaagt acgacggcac catcaccaac
     7981 aacatcagct accccctgac gcgggcgctg tacggggccc gcattcgcca gcccatcggc
     8041 ggtgacttcg cggtctccgg cgacctggtg cagcgctacc tggagctcga cacgttcgac
     8101 gagctgaccg cgcgcttcgg gatcgacatc tggatgacgc actcggccat caacgagggc
     8161 ttcgcggtct gccaggcgcg gctgggcgcg aagatccacg acgccaagga tccggcctcc
     8221 gacctggggc cgatgttccg gcaggtcgtc ggcacgctgt tcaacctggc cggccactac
     8281 gaagaccgat ggctccgggt ccgcggctcg cacccgatcc cggagtacgg cttcgagcgg
     8341 gtcatcagcc cggaggagat cgcggtcaac cacgccaagc tgatcgacaa cctcgagggc
     8401 gcgcgcctga cccagcgcga cgtctggtcg gagatgctgg cgccggagca gctggaacgg
     8461 gtgatgacca tcgactacgg cggcgagccg gccgacttcc acttcagcgc cgagctgtgg
     8521 gtgcgctgcc tgtacgacgc gctcgtggcc ttccatcgcc cggggaccga tcgcgagcgg
     8581 ctgcttgccg cgctgacgcc actgtatttc ggccgcacgg cgggcttcat cagcgacacg
     8641 ctggagatga ccaccgacca ggcggagcgg gtgatcgagg agcaggcgcg gacgttcgag
     8701 gagctcaagc cgtacctggt cgcccgctgg cgcgagatgc gcgccctggc ggagcagcgg
     8761 accgcctgat ggccgcagcc cggcgcaagg cgccctaccg catcctgctg ccgctcgcga
     8821 acccgcggac ggcgcgcgac ctggtccgca tcggtgccgg catcgccaac gggcggccga
     8881 ccgagatcac cgccctgggc atcgtcgagg tgccggaggg cgtcagcctc tccgagggcg
     8941 cgacgcacgc ccgcacccag cggcgcctgc tgcagcgggt cctcgatttc ggcgacgagg
     9001 agggggtgga gctccggacg ctggtccgca tcggccgtca cgccgccgac ggcgtgatcg
     9061 aggccgtcgg cgaggaatcg gccgacctgg tgatcttcgg ctggggcggt ccgcccaccc
     9121 ccgcccagct ggcccgggcc gaggcggccg aggcgggcga gccgtcgccg gccctcgagc
     9181 cgccggcgcc gcgtcccgtc ttctcgccga ccatcgacgc ggtcgtgcgc gaatcgccat
     9241 gcgacatcgc ggtcgtgaag cagcgcggcc tcgagcacgt ggagtcgatc ctcgtcccgg
     9301 tccgcggcgg tccccacgcc gagctggcca tgcgcatcag ccgcgacctg gccaagcgct
     9361 tcggggcgaa gatcgtggtg ctgcacgtcg tgcccaaggg cgtgggcgag cgcgccgtgg
     9421 agcgggagca ggcggcgctg gacgccttcg tccgcgagca cgcgggcacg cgccgcgcga
     9481 ccggcctgat ccgcgaggcg tcatcggtca ggcaggcgat catcaaggag gccgcgcacc
     9541 acgggctggt ggtgatgggc gcatcggcgc atccgaccaa cgcgtcaccg gatggtcggt
     9601 acctgttcgg gacgctggcg gaggcggtgg ccagcaaggc caagccgacc gtcatggtgg
     9661 tcaagaccaa gcagtcgctg gggctggcca ccttcgagga gctgcgcgcc agcgagggga
     9721 cgctggccca tgccgatgcc ttcgtcgagc gcagccgatc gctccccgtc atcgtcgaca
     9781 agtggttcgc cgagaacacc ttccacgccc acgagttcgc cgacatccgc aagctcctcg
     9841 cgctcaagga gcgccacggc gtcacgatca gcgtggcgct gccggcgctc aacgaggaga
     9901 agaccatcgg caccgtcatc aagcgggtca agggcgcgct gatggaccgc gtgccgctca
     9961 tcgaccagct ggtggtcatc gactccgaca gcgaggaccg caccgtcgac atcgcgggtg
    10021 agctgggcgt gccggtgttc cggcaccccg agatcctccc cgagaccggg tcgtaccggg
    10081 gcaagggcga ggcgctgtgg aagagcctcc atgtcctgga cggcgacatc gtggcgtgga
    10141 tcgacacgga catcagcaac atccagccgc gcttcgtgta cgggctgctc gggccgctgc
    10201 tgcgcgagcc ccgcatccag tacgtcaagg gcttctacca gcgaccgatt cgcgagggcg
    10261 acaagctgca ggccgagggc ggcggccgcg tgacggagct catggcacgg ccgctgatca
    10321 acctcttctt cccggagctg tccgggatca tccagccgct gtccggcgag tacgcggggc
    10381 gccgcgccct gctcgagcag ctgccgttct tcaccggcta cgccgtcgag atcgggctgc
    10441 tgatcgacat cctcgaggcg gctggcctgt cggccatcgg ccaggtcgat ctcgagcgac
    10501 ggatccaccg caaccagcca ctcggcaacc tgtcgcagat gagctacgtg atcctgcagg
    10561 gcgccatccg caagctggag gagcggcacc ggctcgagct gctgaccgag gtggggcgcg
    10621 gcatgaagct gattcacacc ggcaaggaga acttcagcct cgaggtgcgc gagatcggtg
    10681 acgacctgcg cccgccaatc gtgacggtcc ccgcctacgc cgagcgtcgc cgcgagctga
    10741 agtcgcgctg acgccacgat ccgagcgggg ccgataggca gggtcgccac gggctgacgg
    10801 acccttcagt gccgatgccc cgcggcccag catcggtgca tgaccagctc tcccgtggcg
    10861 cccacggcgc cctcgcccga cgcccgcgcg ccgcgcccga tcatccgcgt ggccgacgag
    10921 ctgggcttgc tgcccgacga ggtcgagctc cacgggcggc tcctggccaa ggtctcgctg
    10981 ggcgccctga gccgcctggc cgatgcgccc ctcgggcgcc tggtgctggt caccgccatg
    11041 acgccgacgc cgttcggcga gggcaagacc acgaccgcca tcggcctggc ggacgccctc
    11101 gccgccctgg ggctgcgctc gatcgttacg ctgcggcagc cgacgctggg accggtattc
    11161 ggcgtcaagg gcggggccgc cggggccggc cgcgcgtcgc tggttccgcg ccaggaggtg
    11221 aacctgcacc tcaccggcga tgcgtacgcc gtcggcgcgg cccacaacct ggccgccgcc
    11281 gtgctcgacg cccacctgca ccacggcaac gtgctgggca tcgacccgac gaccatcacg
    11341 tggccgcgcg tcaccgacca caacgaccgg gcgctgcgcc atgcccgcat cgccatcggc
    11401 gaggtcgggg agcggga
//
LOCUS       DASTYP010000088         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1718836, whole genome shotgun sequence.
ACCESSION   DASTYP010000088 DASTYP010000000
VERSION     DASTYP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718836"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VFV59_04245"
     CDS             <1..1005
                     /locus_tag="VFV59_04245"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325073.1"
                     /translation="MVMALWLGLVNAVDMPVRQALAADLVPRELLPNAIALNSMAFNS
                     ARVVGPALAGVIIAVGASLTGSDEFGVAVNLAANAVTYGAVLAGLVLMNPHEIRRMER
                     PERHPPVLESLREGFWYAVRTPLVLWCLVLLGGIAAFGFNFQILLPLFAQQVLHVGAD
                     GYGALFAAMGVGSLSGSMTLAFMRQRRAIPLMLIGGAVFSLLLVAIGLARTAWAVVPL
                     VMAAGYASMLMINTINATVQANVSDALRGRVMSFYVTVFAGSAPLGGLFAGAVAEAWG
                     APAAFEMGAVLSIATVGIVAWGLRGARARGSLGVTTLDTSARAPGARRPDAGSASPAA
                     "
     gene            complement(991..1494)
                     /locus_tag="VFV59_04250"
     CDS             complement(991..1494)
                     /locus_tag="VFV59_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5325074.1"
                     /translation="MASSPARSPRPASTTRGAPREEPSLEASLIATVAPLMRHLLAHA
                     RRRSTWTELTYQQYNVLRMIDTMGPQAQADVARRLMVTAPVVTRLASTLADAGLVVRQ
                     DDPNDRRAVLLALTVAGRRRVRAMRRDLLEAAREILGPIPEERRAAVSAALEELQVLV
                     PQHSRRR"
     gene            1482..2714
                     /locus_tag="VFV59_04255"
     CDS             1482..2714
                     /locus_tag="VFV59_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325075.1"
                     /translation="MRTPSSLDRSSPARAGLTLALLSVAHAAIHAQSALLPLIYPIVI
                     VEFGLTERDIGTFIAVTTAVGGGMQLAYGFLTRYVSRPALLAGGQLVFGTGLLIGGLS
                     QSIGQLLGSISLARIGSSPQHPVGNALLSDLFRPERRGFAISTHIAGGNVGTIMVPFV
                     GGALLLALGWQATLAVFGIPALVIGVVLALVVRERHADYRERARASGSVREHLRAVAG
                     RADLRRILAASLVAAGGRGLDIVAPFMVLYLSGPLGFDDDTVHLLYALLLVGAIVGPL
                     LAGVISDRFGRRPTLIAYYLLSAAGIVAFVAAGANLLLLVPLLLPFGTAVFSESPVLQ
                     AYLADRALGPMRDVAFAVYFTFAFGIGAFWAFVIGFVADVAGYPVAFGVMAASYLAAA
                     ALLATIRGSDPATEEAVA"
     gene            complement(2711..3286)
                     /locus_tag="VFV59_04260"
     CDS             complement(2711..3286)
                     /locus_tag="VFV59_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEU5325076.1"
                     /translation="MDAFLLSFGVILLAELGDKSQLMALAFAARYRAWTVLLAVTIAT
                     LIVHAGSVLLGRTFALALPTSAIQIVAGVAFFAFAAWTIRGDELGESDESRARRTGQW
                     ALLTIGTAFFLAELGDKTMLATITLATTEEPWGTWLGSTVGMVAADAIAIAAGALMGS
                     RLPERAIKLFAAGAFVVFGVLLIAEGLGLIG"
     gene            3574..>4722
                     /locus_tag="VFV59_04265"
     CDS             3574..>4722
                     /locus_tag="VFV59_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5325077.1"
                     /translation="MTTEDRTDAATIENLLREDRTFPPPADFVSNALLSDSAIYERTA
                     TEEGFRAFWTEEGNRLDWMEPWTDLYEWNAPYAKWYVGGRLNVSVNCLDRHVDAGLGD
                     RVAYFWEGEPGDTRTITFAQLLDEVCRFTNALRGLGVKKGDKVAIYMPMIPELPVAML
                     ACTRIGAVHSVVFGGFSADALAGRIDDAQCTVVITADGGYRKGAVVPLKHNADEAIAR
                     TSSVEHCVVVRRTGEDVGWYRDRDHWYDELIADQPTTADAEPMDAEDLLYLLYTSGTT
                     AKPKGIMHTTGGYLAGVAATHRYIFDLHPETDVYWCMADIGWVTGHSYIVYGPLANGT
                     TGIIYEGAIDFPEKDRWAQIAAKYRATILYTAPTAIRALMKYGHEQFAD"
BASE COUNT          644 a         1660 c         1677 g          741 t
ORIGIN      
        1 atggtcatgg cgctgtggct gggcctcgtg aacgcggttg acatgccggt ccgccaggcg
       61 ctcgcggccg acctcgttcc ccgcgagctg ctcccgaacg cgatcgcgct caactcgatg
      121 gcgttcaact cggcgcgcgt cgtcggaccg gcgctggccg gcgtcatcat cgcggtcggg
      181 gcctcgctca ccggttccga cgagttcggc gtggcggtga acctcgccgc gaacgcggtc
      241 acctacggcg cggtgctggc cggcctcgtc ctcatgaacc cgcacgagat ccgtcgcatg
      301 gagcggccgg agcgacaccc gcccgtgctg gagtcgctgc gcgagggctt ctggtacgcg
      361 gtgcgcaccc cgctcgtgct gtggtgcctg gtgctgctgg gcgggatcgc ggccttcggc
      421 ttcaacttcc agatcctgct gccgctgttc gcgcagcagg tcctccacgt cggcgccgac
      481 ggctacggcg ccctgttcgc cgccatgggc gtggggtcgc tttccgggtc gatgacgctc
      541 gccttcatgc ggcagcgccg ggcgatcccg ctgatgctca tcggcggcgc cgtgttctcg
      601 ctgctcctgg tggcgatcgg cctggcccgc acggcctggg ccgtggtgcc gctcgtcatg
      661 gccgccgggt acgcgagcat gctgatgatc aacacgatca acgcgacggt ccaggccaac
      721 gtcagcgacg cgctgcgcgg tcgcgtgatg agcttctacg tgaccgtgtt cgccggatcc
      781 gcaccactcg gcggactctt cgcgggcgcg gtcgccgagg cctggggcgc gccggccgcg
      841 ttcgagatgg gcgccgtcct ctcgatcgcg acggtcggaa tcgtggcgtg gggattgcgc
      901 ggtgcgcgcg ctcgcggctc gctgggggtc accacgctgg acacgtccgc ccgggcaccc
      961 ggcgcacggc ggccggacgc cgggtcggcg tcaccggcgg cgtgagtgct gggggaccag
     1021 gacctgcagc tcctcgagcg cggcgctgac cgccgcccgc cgctcctccg ggatggggcc
     1081 caggatctca cgtgcggcct ccagcagatc gcgccgcatg gcgcggacgc gacggcgtcc
     1141 ggcaacggtc aaggcgagca gcacggctcg gcggtcgttc gggtcgtcct gccgcacgac
     1201 caggccggca tcggccagcg tcgaggcgag tcgggtcacc accggggccg tcaccatcag
     1261 ccggcgcgcg acgtcggcct gggcctgcgg gcccatcgtg tcgatcatgc gcagcacgtt
     1321 gtactgctgg taggtgagct cggtccacgt cgatcgtcgg cgggcgtggg ccagaaggtg
     1381 ccgcatgagc ggggcgaccg tggcgatcag gctcgcctcg aggctcggct cctcgcgggg
     1441 agcgcctcgg gtcgtcgacg cgggccgagg gctgcgcgct ggtgaggacg ccatcgtcgc
     1501 tcgatcgtag cagtccggcg cgtgccgggc tgacgctcgc cctcctcagc gtcgcccacg
     1561 ccgccattca cgcccagtcg gcgctgctgc cgctcatcta cccgatcgtg atcgtcgagt
     1621 tcggcctcac cgagcgcgac atcggcacgt tcatcgcggt caccacggcc gtcggtggcg
     1681 gcatgcagct ggcctacgga ttcctgactc gctacgtctc ccggccggcg ctgctggccg
     1741 gtgggcagct cgtcttcggc acagggctgc tgatcggagg cctgtcgcag tccatcggcc
     1801 agctgctcgg ctcgatcagc ctggcgcgca tcggttccag cccgcagcac ccggtcggca
     1861 acgcgctcct gtcggacctc ttccgacccg agcgacgcgg cttcgccatc agcacccaca
     1921 tcgccggcgg gaacgtgggg acgatcatgg tcccgttcgt cggtggtgcc ctgctgctcg
     1981 ccctcggctg gcaggcgacg ctcgccgtgt tcggcatccc ggcgctggtc atcggcgtcg
     2041 tgctcgcgct ggtggtgcgc gagcgccatg cggactaccg cgagcgggcg cgcgcgtcgg
     2101 gctcggtccg agagcacctg cgggcggtcg ccggacgggc agacctgcgg cgcatcctcg
     2161 ccgcctcgct ggtcgcggcc ggaggacgag gactggacat cgtcgccccg ttcatggtcc
     2221 tgtacctgtc gggcccgctc ggctttgacg atgacacggt gcatctgctc tacgccctgc
     2281 tcctggtcgg ggccatcgtc gggccgctgc tggccggcgt gatctcggac cgattcggcc
     2341 gccggccgac gctcatcgcc tactacctgc tgtcggcggc cgggatcgtg gcgttcgtcg
     2401 cggcgggagc caacctcctg ctcctagtcc cgctgctgct gcccttcggc accgcggtct
     2461 tcagcgagtc gccggtgctg caggcctacc tggccgatcg ggcgctaggg ccgatgcgtg
     2521 acgtggcctt cgcggtgtac ttcacctttg ccttcggcat tggcgccttc tgggccttcg
     2581 tcatcggctt cgttgccgac gtcgccggat acccggtcgc gttcggcgtg atggccgcgt
     2641 cctacctggc cgcggccgca ctgctggcca cgatccgcgg ctcagacccc gccacggagg
     2701 aggccgtcgc ctagccgatc agaccgaggc cctcggcgat gaggagcacg ccgaagacga
     2761 cgaacgcccc ggcggcgaac agcttgatcg cccgttccgg aagccgcgat cccatcagcg
     2821 ccccggccgc gatggcgatc gcgtcggcgg cgaccatgcc gacggtcgat ccgagccagg
     2881 ttccccacgg ctcctcggtg gtcgcgaggg tgatggtcgc gagcatggtc ttgtcgccca
     2941 gctccgccag gaagaaggcc gtgccgatgg tcaggagcgc ccactgcccg gtgcggcgcg
     3001 cgcggctctc gtcagactcg ccgagctcgt cgccgcggat ggtccacgcc gcgaaggcga
     3061 agaaggccac gcccgccacg atctggatgg ccgaggtcgg cagcgcgaga gcgaacgtgc
     3121 gcccgagcag gacggagccg gcgtgcacga tcagggtcgc gatggtcacg gccagcagga
     3181 ccgtccaggc gcggtagcgt gcggcgaagg cgagcgccat gagctggctc ttgtcgccaa
     3241 gctcggcaag gaggatcacg ccgaaggaga ggaggaatgc gtccaaggct gactccggta
     3301 ggggatcggg cgacgcgcgg acgcttcgac ccgacacccg atgaggtatc gacagtcgaa
     3361 ggtctcgtcc atccgcgtgc gcggacccgg cgaccgggcg catcggcgcc agcgtgtcga
     3421 tcggccggtg gaggtctact ccccttcgca ggcgccagtg tcggcgttgg gtgccggggc
     3481 gtcaaacgcc cgcgggcatg gcggccggtg cctgaccgta tatcggtata tacgtgccga
     3541 cgccacggca tggggcgcgg ctagaatcgc cgaatgacga ccgaagaccg aaccgacgcc
     3601 gcgacgatcg agaacctcct gcgcgaggac cgcaccttcc cgccgccggc ggacttcgtg
     3661 tcgaacgccc tcctgagcga ctcggcgatc tacgagcgca cggcaacgga ggagggcttc
     3721 cgcgccttct ggacggagga ggggaaccgg ctcgactgga tggagccgtg gaccgatctc
     3781 tacgagtgga acgcgccgta cgcgaagtgg tacgtcggcg gcaggctgaa cgtgagcgtc
     3841 aactgcctcg accgccacgt ggacgccggc ctcggcgatc gcgtcgccta cttctgggag
     3901 ggtgagccgg gcgacacgcg caccatcacc ttcgcacagc tgctcgacga ggtctgccgc
     3961 ttcaccaacg ccctgcgcgg gctgggcgtg aagaagggcg acaaggtcgc gatctacatg
     4021 ccgatgatcc ccgagctgcc ggtcgccatg ctggcctgca cccgcatcgg tgccgtgcac
     4081 tcggtcgtct tcggtggctt cagcgccgat gcgctcgccg ggcgaatcga cgacgcgcag
     4141 tgcacggtcg tgatcaccgc cgacggcggc taccgcaagg gcgcggttgt cccgctcaag
     4201 cacaacgcgg acgaggccat cgcgcggacc tcatcggtcg agcactgcgt ggtcgtgcgg
     4261 cgcaccggcg aggacgtcgg ctggtaccgg gaccgcgacc actggtacga cgagctcatc
     4321 gcggaccagc cgaccacggc tgatgcggag ccgatggacg ccgaggacct gctgtacctg
     4381 ctgtacacct ccggcaccac ggccaagccg aaggggatca tgcacaccac cggcggctac
     4441 ctggcgggcg tggcggccac gcatcggtac atcttcgacc tgcatccgga gaccgatgtc
     4501 tactggtgca tggccgacat cggctgggtg accgggcact cgtacatcgt gtacgggcct
     4561 ctggccaatg gcacgaccgg gatcatctac gagggcgcca tcgacttccc cgagaaggac
     4621 cgatgggcgc agatcgcggc gaagtaccgc gccaccatcc tctacaccgc gccgaccgcc
     4681 atccgcgcgc tgatgaagta cggccacgag cagttcgccg at
//
LOCUS       DASTYP010000089         4285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1609129, whole genome shotgun sequence.
ACCESSION   DASTYP010000089 DASTYP010000000
VERSION     DASTYP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609129"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2193)
                     /locus_tag="VFV59_04270"
     CDS             complement(<1..2193)
                     /locus_tag="VFV59_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019487661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEU5325078.1"
                     /translation="MTQTQAGSAARSASGARQDKATEILGHAAHPLDVLFAPKSVAVI
                     GATESPGKVGRTLLANLISSPFGGTVFPVNPTRASVLGVKAYPDLASIPDPVELAVIV
                     TPAPTVPGLIEECVAAGVKGAIIISAGFKETGAEGAALEAQVLETARRGRMRIIGPNC
                     LGVMNPLNGFNATFAAGIARPGSVGFVSQSGALITAVLDWAEREQTGFSSVVSLGSML
                     DVGWGDVIYYLGDDPKTKSIVVYMETIGDARAFLSAAREVALTKPIIVIKPGRTEQAA
                     RAAASHTGSLTGSDDVLDAAFRRVGVLRVESIAELFGMAEVLAQQPRPKGPRLSILTN
                     AGGPGVIATDALIGGGAELAPLPDETREALNGVLPAAWSHGNPIDILGDAPPERYRQA
                     VEIAAANPESDGLLVVLTPQDMTDATGTAEQLVGFKLPGGGPMLASWMGGPGVDAGTE
                     LLQKAGIPVFEYPDVAARMFTYLWRSAADLRLLYETPVLAEPDGDGRDGTDGPDGAHA
                     AGRQRVEQTIAKARAERRTLLSEAESKDILAAYGVPVAETRVTESEDEAVAAAEAIGY
                     PVVVKLFSRTITHKTDVGGVKLNLVDADAVREAYRSIRDSVATAKGAEHFNGVTVQPM
                     INYTGYELIVGSSIDPQFGPVLLFGTGGTMVEVIRDRALALPPLTTTLARRMIERTQV
                     FKALKGIRGREPIDLAALEQLLVRFSRLVVEHPWISEIDINPLLASPQR"
     gene            complement(2231..3913)
                     /locus_tag="VFV59_04275"
     CDS             complement(2231..3913)
                     /locus_tag="VFV59_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEU5325079.1"
                     /translation="MTDIVVRPGRRWPAFLVAAAAALLGAFVAPSSWPAGPASLELRL
                     GTDVVGSATVDLGSTDPAEVSLGVGTLTLPGGAPIDEKLTARVQLTDETREPGQLSAW
                     LVLPDGRDELLPVIRTDADAGILEIERWPPYRADAVLALLGVVVVLWVTEAVPLFVTS
                     LAIPVVLVAAGVGGPTQSLQNFFHPIIALFFGGFLMAEAMRRTGLDHLAAISIIARAG
                     RSPVTLFAAMIGVSAFLSLWMSNTAAVALVLPIALAVTAPMESIGYRRAIVLGIAYAA
                     TVGGVGSAIGTPANPLAITFLRDFVGRELTFVDWFAIGLPWVLIFLPLMSLYLWWRVG
                     EAPDRSRFVDARAVAHDELAAAGRPTRPQLTVMAVFLAVIAVWLTEPIHGLETGIVAL
                     GGAVALAVLGLVEPDDLGRISWASLLTFGGGLTLGVFVVQTGTSDWIATQLGGLADLP
                     GPLVVAVVAGVALLLTTIASNTASAAMTIPLAIPLATIVGVDPVVLVLAVAIASSIDF
                     ALVIGTPPTMLAYSTGLYTPGGIFRVGAVLDVVGLLVLVTVVAWIWQLVGIA"
     gene            complement(3983..>4285)
                     /locus_tag="VFV59_04280"
     CDS             complement(3983..>4285)
                     /locus_tag="VFV59_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325080.1"
                     /translation="RFQLLHLAVEAVGGGLVVVGAGLATVVGRHRLGWTLAYFGLLVT
                     ITLGDLISFYVRQFDSIVVVLGHLVLLTAVVGARERLRSERDASGPEPATVMLDRT"
BASE COUNT          669 a         1526 c         1543 g          547 t
ORIGIN      
        1 tcgctgggga gacgcgagca gcgggttgat gtcgatctcg ctgatccacg ggtgctcgac
       61 caccagccgg ctgaagcgca cgaggagctg ctccagggcc gccaggtcga tcggctcacg
      121 tccgcgaatg cccttgagcg ctttgaagac ctgcgtccgc tcgatcattc ggcgcgccag
      181 ggtggtggtc agcggcggca gcgccagcgc ccggtcgcgg atgacctcga ccatggtgcc
      241 tccggtgccg aacagcagca ccgggccgaa ctgcggatcg atgctgctgc cgacgatcag
      301 ctcgtagccg gtgtagttga tcatcggctg gaccgtcacg ccgttgaaat gctcggcccc
      361 cttggcggtc gccacggagt cgcgaatgga ccgatacgcc tcgcggaccg catcggcgtc
      421 gaccaggttg agcttcacgc cgccgacatc ggtcttgtgg gtgatcgtgc gggagaagag
      481 cttgaccacc accgggtagc cgatggcctc ggccgcggcc accgcctcgt cctcggactc
      541 ggtgacgcgc gtctcggcca ccgggacgcc gtaggcggcc aggatgtcct tcgactcggc
      601 ctcgctcagc agcgtccgcc gctcggcgcg cgccttcgcg atcgtctgct ccacgcgctg
      661 gcggccggcg gcatgcgccc cgtcggggcc gtcggtcccg tcgcgaccgt ctccatccgg
      721 ctcggcgagg accggcgtct cgtacagcag ccgcaggtcg gcggccgatc gccagaggta
      781 ggtgaacatc ctggcggcga cgtcggggta ctcgaagacc gggatgcccg ctttctgcag
      841 caactcggtt ccggcatcga cgcccgggcc gcccatccac gacgcgagca tcggtccacc
      901 accggggagc ttgaagccga cgagctgctc ggcggtgccc gtcgcatcgg tcatgtcctg
      961 cggagtgagg acgaccagga gcccgtcgct ctccgggttg gccgccgcga tctcgacggc
     1021 ctggcggtag cgctcgggcg gggcatcgcc caggatgtcg atggggttgc cgtggctcca
     1081 cgcggcggga aggacgccgt tgagcgcctc gcgggtctcg tcgggcagcg gggcgagctc
     1141 cgctcccccg ccgatcagcg cgtcggtcgc gatcacgccc gggccgccgg cgttggtgag
     1201 gatcgagagg cgcggcccct tggggcgcgg ctgctgggcc aggacctccg ccatgccgaa
     1261 cagctcggcg atgctctcga cgcggaggac gccgacgcgc cggaacgcgg cgtccaggac
     1321 gtcgtcgctg cccgtcagcg accccgtgtg cgacgcggcg gcgcgtgccg cctgctccgt
     1381 gcgacctggc ttgatgacga tgatcggctt ggtcagcgcg acctccctgg cggcggagag
     1441 gaaggcccgc gcgtcgccga tggtctccat gtacacgacg atcgacttcg tcttcggatc
     1501 gtcgccgagg tagtagatga cgtcgcccca accgacgtcg agcatcgagc ccagcgagac
     1561 gacggagctg aagccggtct gctcgcgctc cgcccagtcg agcaccgcgg tgatcagcgc
     1621 gcccgactgg ctcacgaacc cgacgctccc gggacgcgcg atgccggcgg cgaacgtcgc
     1681 gttgaacccg ttgagcgggt tcatgacccc caggcagttg ggaccgatga tccgcatccg
     1741 cccgcggcgc gccgtctcga gcacctgcgc ctcgagcgcc gccccctcgg cgccggtctc
     1801 cttgaagccg gccgagatga tgatggcgcc cttcacgccg gcggcgacgc actcctcgat
     1861 cagccccgga acggtcggcg ccggggtgac gatgacggcc agctccaccg gatcggggat
     1921 ggaggcgagg tcggggtacg ccttgacgcc gaggacgctg gcccgggtcg ggttgaccgg
     1981 gaagacggtc ccgccgaacg gtgagctgat gaggttggcg agcagcgtcc gcccgacctt
     2041 gcccggtgac tccgtcgcgc cgatcaccgc caccgacttc ggggcgaaga ggacgtcgag
     2101 cgggtgcgcc gcgtggccga ggatctcggt cgccttgtcc tgccgagcgc cggatgcgga
     2161 gcgcgcggcg ctcccggcct gggtctgcgt catggggttc tcctcgttcg gtccacggta
     2221 ctcctcggcc tcaggcgatg cccaccagct gccagatcca cgccaccacc gtcaccagga
     2281 ccagcaggcc gaccacgtcc agcaccgccc cgacgcggaa gatcccgccc ggggtgtaca
     2341 ggccggtcga gtaggccagc atggtcggcg gcgtgccgat gaccagcgcg aagtcgatgg
     2401 acgaggcgat ggccaccgcc agcaccagca ccaccgggtc gactcccacg atcgtcgcca
     2461 gcgggatcgc cagcgggatg gtcatggcgg ccgatgccgt gttcgacgcg atggtggtca
     2521 gcagcagcgc cacccccgcg acgacggcca ccacgagcgg accgggcagg tccgcgagcc
     2581 cgccgagctg cgtcgcgatc cagtcggacg tcccggtctg caccacgaag accccgagcg
     2641 tcagaccgcc gccgaaggtc agcagcgacg cccacgagat gcgccccagg tcgtccggct
     2701 cgaccaggcc gaggaccgcg agcgcgacgg cgccaccgag cgcgacgatc ccggtctcca
     2761 gaccgtggat cggctcggtc agccagacgg cgatgaccgc caggaagacg gccatgacgg
     2821 tgagctgcgg ccgggtcgga cggcccgcgg cggcgagctc gtcgtgggcc acggcacgcg
     2881 cgtccacgaa gcgcgaccgg tccggcgcct cccccacccg ccaccacagg tacaggctca
     2941 tgagcggcag gaagatgagg acccagggca ggccgatggc gaaccagtcg acgaaggtga
     3001 gctcgcggcc cacgaagtcg cgcaggaacg tgatggccag cgggttggcc ggcgtcccga
     3061 tggcgctgcc cacgccgcca acggtcgcgg cgtaggcgat cccgaggacg atcgcgcgcc
     3121 ggtagccgat gctctccatc ggcgccgtca cggcaagggc gataggcagg accagcgcca
     3181 ccgcggcggt gttcgacatc cacagcgaga ggaaggcgga gacgccgatc atcgccgcga
     3241 acagcgtcac cgggctgcga ccggcgcgcg cgatgatcga gatcgccgcc aggtggtcga
     3301 gccccgtccg gcgcatcgcc tcggccatga ggaagccgcc gaagaagagc gcgatgatcg
     3361 ggtggaagaa gttctggagt gactgcgtcg ggcccccgac gccggcggcg accagcacca
     3421 ccgggatggc cagcgaggtg acgaagagcg gcaccgcttc ggtgacccac agcaccacga
     3481 ccacgccgag gagcgccagg accgcatcgg cacggtaggg cggccagcgc tcgatctcga
     3541 ggatcccggc gtcggcatcg gtccggatca ccggcagcag ttcgtcccgg ccgtcaggca
     3601 gcaccagcca cgcgctcagc tggccgggct ctcgtgtctc gtcggtgagc tgcacacggg
     3661 ccgtgagctt ctcgtcgatc ggcgcgccac cggggagggt cagcgtgccg acgccgagcg
     3721 agacctccgc cgggtcggtg ctgcccaggt cgacggtggc ggagccgacg acgtccgtgc
     3781 ccaggcgcag ctcgaggctc gccggtccgg ccggccacga ggaaggcgcc acgaacgcgc
     3841 cgagcagcgc ggcagcggcg gcgacgagaa aggccggcca ccgacgcccg gggcggacga
     3901 cgatgtcggt cacctcgcca gttccgcaca ccccgggtcg tggctcggag ggccggacgt
     3961 cggaatccgt ccgggccgaa ggtcaggttc ggtcgagcat caccgtcgcg ggctcggggc
     4021 cggaagcgtc tcgctcggaa cgaaggcgct cgcgcgcgcc gaccaccgcg gtcagcagca
     4081 ccaggtgacc gagcacgacg acgatggagt cgaactggcg cacgtagaac gagatcaggt
     4141 cgcccagcgt gatggtgacc agcagcccga agtaggccag cgtccagccc agccgatgcc
     4201 ggccgaccac ggtggccaac ccggccccca ccaccaccag cccaccgccg accgcctcga
     4261 cggcgaggtg caggagctgg aagcg
//
LOCUS       DASTYP010000090        17448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1390601, whole genome shotgun sequence.
ACCESSION   DASTYP010000090 DASTYP010000000
VERSION     DASTYP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390601"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..363)
                     /locus_tag="VFV59_04285"
     CDS             complement(115..363)
                     /locus_tag="VFV59_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325081.1"
                     /translation="MEELNEIGRIEIQPGWTVLDADGQTVGTVDEAGEQSFTVRVEGP
                     VDATIGVAYADIESADDGRVVLGVSREDLDAAVDVGAP"
     gene            473..1987
                     /locus_tag="VFV59_04290"
     CDS             473..1987
                     /locus_tag="VFV59_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5325082.1"
                     /translation="MSRLLAVTFVVLALILAACESDSSASEGASGGSIQPGASGEGQA
                     GGSAGASASGEEEAAGPSLTDTTLAVETVTTDLSEPTSMAFLGENDFFVTEKSTGLVQ
                     HVVDGQLGDPAADLAVNFFDERGLLGIALHPDFAENGYVYLYWTATDGLGEGDQGMFG
                     DDTNDEFAVPDLGNRVDRFVWDGSQLTWDRNIVQLRSNTLDTDTSGRIRGNHDGGAIV
                     FGQDGKLYIIIGDQNLRGQYQNLADGPAPDDMNFAGMIVRVNDDGSIPEDNPFHGVGA
                     EMGGEAGENVQLTWAYGIRNSFGIAVHPETGDIWETENGDDSWDEVNIFPAGSNSGWW
                     QLMGPPERFDEFRQIETESEDGTDNPDVPPDQLAESGDEAQSRLFALDGSQYEPPIFS
                     WRYPPAATAIGFVVDDALGESSANTAWIGSVLTDSIYRYPLAADGSGFDFGDDEGLAD
                     GVDDNSAKGDPGESADYMIGSGFGVVTHIVQGPDGMLYVTSLSNRAVYRIGPAS"
     gene            2200..4332
                     /locus_tag="VFV59_04295"
     CDS             2200..4332
                     /locus_tag="VFV59_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5325083.1"
                     /translation="MHRHRALLPRTMAALAAALLLLASFASIATAQSLTDRVTDEAGV
                     LDGGGRSEALNAIEQLESAANIQLWALFVGSTGGEPIDDYTQRLVDDNGLGVNDALFV
                     VAVDDRRYQLWISDSLTDQVTTDEQDAILAERVEPALRDGDWSGSVVAAAEGLADAAT
                     TSGGGGGGGGGGGDGGDGGTGGGDQPAGEPSSGPSFFVVLLAVVLIGVGGWLLWSRLQ
                     AGRAREEDDRERERRLRGLSQQANALLIETDELLRHDAQELGFVEAEFGSDAADPFRE
                     ALAKARAELQAAFRVRQQLDDDQPETPPEREKLLNEIVSRCASARKLVEEQTERFREL
                     RDLERRAPEILAEQERKIAAVEARIPDVEQSLESLASDASGSSQAVRGNVAEARKRIE
                     LARGGVTEGQAALGRNEGPKAARAAKASQDALAQASGLLDAVDREAATLSEARTALDG
                     AVAQARADLEAASQAVAGASDRDQADELAAAQAKLQAADAALTGTPRDLVLAYRLARE
                     AESQAEQVVARVHEGEQKRAKELAAVDAGIRAAELSLDRASEFIAARRHGVGRRPRTA
                     LSEADVALDEARRLRQADPAAALASAKRATQLADEAYTRARNDFASVDRRGYGGTVII
                     DGQPYSSGRSSWGSDVGGAIIGGIIGSILSGGGRGGGWGGGFGGGGGFGGFGGGGGGG
                     IGRGLGGGGRSFGGGFGGGGGRSRGGGW"
     gene            4392..5102
                     /locus_tag="VFV59_04300"
     CDS             4392..5102
                     /locus_tag="VFV59_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEU5325084.1"
                     /translation="MAQTTILGRIGQLVRANINALLDSAEDPERMLDQLVRDFTNNIA
                     EAEEAVAQTVGHLRLLEDDLREAQSAQTEWADKARAAARKADEMRSGGNSSEADRFTE
                     LAKIAIRRQISFENQVKTFQGQIEQQTQLTDQLKDGLNKLRLKREELVQKRDELVSRA
                     KMASAQRQVQEAVREVSVMDPTSELNRFEERIRKEEAMARGMAEVSASSLEEEFASLD
                     DADTEAEVEARLASMQGA"
     gene            5326..5790
                     /locus_tag="VFV59_04305"
     CDS             5326..5790
                     /locus_tag="VFV59_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEU5325085.1"
                     /translation="MATRTDAHHTGHTRRAARRPTRAPADRREGSARGLADRFRESAE
                     IITHAVGTPWALGVAALIVVAWGVTGPVFGFSNTWQLVINTGTTIVTFLMVFVIQASQ
                     NRDSRAIHLKLDELLRATKGARNRLIDAEHEVERDMERDERDLQRRARSRRG"
     gene            complement(5787..6290)
                     /locus_tag="VFV59_04310"
     CDS             complement(5787..6290)
                     /locus_tag="VFV59_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325086.1"
                     /translation="MTRRHLVADLGEVEPRPLQLTVRTAERSARTALAELMLDAYQGT
                     IDADGSETLDVAQGEIDHYLRGGSGRPMLGHSRLAYDGERLVSAVLVSEFEGTPLIAY
                     VCTASDHKGRGLADGLVRLAMRSLAQAGHRRVHLWVTAGNEPAERIYERLGFHGVGDA
                     NVRADQA"
     gene            6436..7314
                     /locus_tag="VFV59_04315"
     CDS             6436..7314
                     /locus_tag="VFV59_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325087.1"
                     /translation="MASAHAAVHRLPSIFAVVVTAALLLLAATPARVAAADEFQPGSG
                     VVRQGPTRPPAQQPPGYDVSYPQCGTSLPEAFAFAVVGVNGGRVFSPNPCLGTGENPS
                     ELEWAGPEAQLYANTGNPGPELSKYWPDGQTTPLECNTAANPGLDTPECAYDYGWNAA
                     ADSYRTAIAAYVSLGWADPDADRTPVANFWWLDVETSNSWRSEHALNIAVLQGAVAYL
                     ESVEVGGIGFYSTPQQWGQITGGTDVFATYPAWHAGARTLRGAMANCRDVPFTGGALV
                     MTQYLERGLDANYLCP"
     gene            complement(7364..8344)
                     /locus_tag="VFV59_04320"
     CDS             complement(7364..8344)
                     /locus_tag="VFV59_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5325088.1"
                     /translation="MTQLAPDATELTGQLEQHRTELTAYAYRMLGSSFEAEDAVQETM
                     VRAWRGLDRFEGRAQLRSWLYRITTNVCLDMLKGRERRARPMDLRPAQSASTPLPQPL
                     EENLWLEPIPDAKVVSADGDPAEVAATRESVRLAFMAALQHLPPKQRAVLILREVLRW
                     RASEVAELLDTSVASVNSALQRARDTLNTRNLTEEAPPAAPDDPETRQLLARYVDAFE
                     RYDMQALTALLAEDAVWNMPPYDLWLQTHEDIVAWCIGPGYACEGSKLIPTTANGSPA
                     FAQYKPDPAGGHAPWSLQVLELRDGKIVGITFFLDTKRWFPLFGLPPHLD"
     gene            complement(8355..8732)
                     /locus_tag="VFV59_04325"
     CDS             complement(8355..8732)
                     /locus_tag="VFV59_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325089.1"
                     /translation="MSRGRFERWRVSIPPGPSRASTPDAWADAVVLVEAGTLEVRCVG
                     GASARFGPGSLLALGWLPLASLRSADPGGVQLLAVRRRLRTDAAWHRELLPVSRWSMA
                     LAALSRRAAASAALGEDGSRGDR"
     gene            complement(8742..10109)
                     /locus_tag="VFV59_04330"
     CDS             complement(8742..10109)
                     /locus_tag="VFV59_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5325090.1"
                     /translation="MTLTRTIDELRAGLRGRVITPDDADYDATRRIALGGTDPRPAAI
                     VRVAGTDDVARTIAHARRIRADLAVRSGGHSGAGHGTADGGIVIDLRDLRRFDLDTDT
                     GTAWADAGLTAGEVTEAAAAHGLAIGFGDTATVGIGGITLGGGIGYLVRKHGMTIDSV
                     LAAEIVTADGEIRTVDVDRDPDLFWALRGGGGNFGVVTRFRYRLHPLDQVVGGMLILP
                     ATPDTIAAFMTAADAAADELSAIANVMACPPMPFVAEEHHGTPVIMALMCWSGAVEEA
                     DAAYAPFRALATPLADMVRPIRYPEMYPPEDPDYHPTAVSRTMFVERIGLEDAAAIVA
                     AIEASDAPMRGAQLRVLGGAAARVPSDATAYAHRASRIMVNVFSFYDGEGDRSRREAW
                     VADLSDALRQSDAGAYVNFLADEGPERVRAAYPGTTWNRLVEVKRRYDPDNVFRRNQN
                     IAPEA"
     gene            complement(10181..10600)
                     /locus_tag="VFV59_04335"
     CDS             complement(10181..10600)
                     /locus_tag="VFV59_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HEU5325091.1"
                     /translation="MTAVIDSGVLYALVDAGDSLHDECVAAVSDERETIVVPMPTLPE
                     VCTLIGSRLGSSRETAFIRYLADSDWRLEPLAGSDIPRVLSIMSEAADHGIGFADAAV
                     ATIAERMGAVRIYTLDRRHFEIVRPRHVERFELLPHR"
     gene            complement(10597..10836)
                     /locus_tag="VFV59_04340"
     CDS             complement(10597..10836)
                     /locus_tag="VFV59_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU5325092.1"
                     /translation="MKRTSILLDPGLLAELEGLARRKGVPTAHVIRGALEAYVAEQTD
                     GERQLPGFVGIGRGPGDVAERIEELLPADLEDPAR"
     gene            10989..12104
                     /locus_tag="VFV59_04345"
     CDS             10989..12104
                     /locus_tag="VFV59_04345"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5325093.1"
                     /translation="MPHVTLTARAHRALFGVAITLTIAVQSAVAPAATFGADAAAQLP
                     SPVPVGDTHPSLEAQLAGALRRARENSNSPGLSVAAISPSGKTWSGANGTFQSGASLN
                     PTDALTIGSVTKTFVAAVVLALVDEGRIQLDVPASTYLPRAMALENGATVRQLLSHTS
                     GIADLYGPAKDVLSGDPWRVMGSNDVLEPIGGDYFAPGTGYAYSNTNYYLLGHIVEVV
                     THHSFNDELAARFTGPLGLASVRLLTAADTVMPAAWTTTFWTSGAMVSTPMDLARWGR
                     ALYGGQVLSEASTGRMLDFSAGHHYGLGAQLLPLGGQEVPGHSGLMYEMTTLLVRLPD
                     GMTLAIVSTQPYTDLEAVLVGNYGGPSLLELLYHLDG"
     gene            12124..12894
                     /locus_tag="VFV59_04350"
     CDS             12124..12894
                     /locus_tag="VFV59_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU5325094.1"
                     /translation="MPTVSRLSITPVRSLALHHPDAVELGPHGVADDRRYLVLTPDDR
                     LFDGTKHGPLVRMRADLAHDPERLTVHLPDGRVVEAVVELGAARQVTIYGRTFTVRAV
                     IGPWATAISGVIGRAVALARVERLPEERDRNPVSIVSEASVHELERQGNDGRPLDPRR
                     FRMLIQLADADPHQEDGWIGRRVRIGSEVVVRVTRPDPRCVITTQDPATGVRDFPTLH
                     AIKAYRGLRDGRHIDFGVYADVEAPGVVRVGDDVSPLD"
     gene            12954..13145
                     /locus_tag="VFV59_04355"
     CDS             12954..13145
                     /locus_tag="VFV59_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEU5325095.1"
                     /translation="MPYDRISDLPDSQVDQYSHHQKEAFLAAFNNALKEYDGDESRAF
                     ATAHAAAKRTPKEDPTPGD"
     gene            13582..14307
                     /locus_tag="VFV59_04360"
     CDS             13582..14307
                     /locus_tag="VFV59_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325096.1"
                     /translation="MSTEQSGPPSEPEGERPTTPSPTPTQLRLQILSTEHWSLLASRG
                     LAWNESFTRASMFLSTLSFAVVALALVGQASGFGQEFRLFALVILPVVLLLGMATSVR
                     MDSANHHDVQCVIGMNRIRAAYLEIAPDLERYFVMGTTDDMAGIVRTMAVLPDRSTRL
                     NLLSASPTVIAVLNSVLAGGIIGLAVMQFGMAGDAAVIAGVLGFIGVATAWAWYSRNL
                     LRHLVHDYEPMFPGADPGTPLRV"
     gene            complement(14315..14545)
                     /locus_tag="VFV59_04365"
     CDS             complement(14315..14545)
                     /locus_tag="VFV59_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325097.1"
                     /translation="MDERRPDRLPSRSSAGALIGGLLAGLEHALTNRPPPPAQIEERY
                     HRPWAALNGVVVEGLDERLERPEPPDRSGARV"
     gene            15113..16465
                     /locus_tag="VFV59_04370"
     CDS             15113..16465
                     /locus_tag="VFV59_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5325098.1"
                     /translation="MSFANLGLTPDLLRAVQAQGYTDPTPVQREAIPLVLQGRDLLAG
                     AQTGTGKTAAFVLPMLQRLAATERRANDGRPRIRALILTPTRELAIQVEQSVRDYGAG
                     ARRSTTIFGGVGMPGQVAALRRGVDIVVATPGRLLDHARQRTIDLRSVEILVLDEADR
                     MLDMGFIRDIRSVLALLPDRRQSLLFSATFSNEIRSLAEGMLDRPASVQVTPRNTPTE
                     LVRQVVHPVDRSRKRELLSHLVRTHAIEQALVFTRTKHGANKLAEQLWKDGIATAAIH
                     GNKSQPQRVRALNDFKAGRVTLLIATEVAARGLDIEQLPHVVNYELPMVPADYVHRIG
                     RTGRAGAEGDAISLVCVDERPLLRDIERLLGEPIATEVIAGFEPDPTAVAEPIRLRSG
                     QHAGRASAPRHRAAPRAHAGAPSGGSAPRRRGGPRRAGASIGNRERGWSSLPGERRRG
                     "
     gene            16476..16985
                     /locus_tag="VFV59_04375"
     CDS             16476..16985
                     /locus_tag="VFV59_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5325099.1"
                     /translation="MVTRVILTGDTHLPRFGRSLPAALVEALTDADLVLHAGDLTDPF
                     VLDLLAEHTPVVAVAGNNDAPALHERLGLTRVVEVEGVRIGLTHGHAGRGRTTPDRAR
                     NAFDDQGLDAIAFGHSHQPVVERVDGRWLLNPGSPTDRRRQPTFSFLRLEVEGRDLRP
                     ELVTYAHRR"
     gene            17078..>17448
                     /locus_tag="VFV59_04380"
     CDS             17078..>17448
                     /locus_tag="VFV59_04380"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5325100.1"
                     /translation="MRHVETTTRVPGSPERAFDLAVDPHRMSEQMPWVGGITEVRGRG
                     DRLGDSFVFRDHVFGRSERAMTVVTACHRPTFQTTETTYDNGFRVIWTMHFSPCEEGT
                     EIRNVVWWAKPDDWVGRVEEL"
BASE COUNT         2533 a         6215 c         6247 g         2453 t
ORIGIN      
        1 acccacggac agctgacgta cgactgtgcc gatcagtacg gcgggtgggt gaacgatttc
       61 ttcgcgaccg ccttcggggg cggcgattga tggctgaagc gcgctgaccg aaggtcaggg
      121 cgcgccgacg tcgacggcgg cgtccaggtc ctcgcggctg acgccaagca ccacgcggcc
      181 gtcatcggcg ctctcgatgt ccgcgtaggc gacgccgatg gtcgcgtcca ccggtccctc
      241 gacgcgaacc gtgaagctct gctcgcccgc ttcgtcgacc gtgccgaccg tctgaccgtc
      301 ggcgtcgagc accgtccatc ccggctggat ctcgatccgg ccgatctcgt tcagctcctc
      361 catgcgatgg cccttcagca tggccggtgc cacgccagcc gggtgggcgt ggcaccgacc
      421 gtgcaccagt ggccagctca acccccatcg ccccctggag tctcacacgc ccatgtcccg
      481 cctgctcgcc gtcaccttcg tcgtcctcgc cctgatcctc gccgcctgcg agagcgactc
      541 gtccgcatcg gagggtgcga gcggcggctc gatccagccg ggcgcatccg gtgagggtca
      601 agccggcggc agcgccggcg catcggcttc gggtgaggag gaggcggcag gcccgtccct
      661 gaccgatacg accctggcgg tcgagacggt cacgaccgac ctgtccgagc cgacctcgat
      721 ggcgttcctt ggcgagaacg acttcttcgt caccgagaag agcaccggcc tggtccagca
      781 cgtcgtcgac ggacagctgg gcgatccggc ggccgacctg gccgtcaact tcttcgacga
      841 gcgcggcctg ctgggcatcg cgctgcaccc cgacttcgcc gagaacggct acgtctacct
      901 gtactggacc gcgaccgacg gcctgggcga gggtgaccag gggatgttcg gggacgacac
      961 caacgacgag ttcgccgtgc cggacctggg caaccgcgtg gatcggttcg tgtgggatgg
     1021 ctcgcagctg acctgggacc gcaacatcgt ccagctgcgc agcaacacgc tcgacaccga
     1081 cacgtcgggg cgcatccgcg gcaaccacga cggcggcgcc atcgtgttcg ggcaggacgg
     1141 caagctgtac atcatcatcg gcgaccagaa cctccgcggg cagtaccaga acctggccga
     1201 tgggccggcg ccggacgaca tgaacttcgc gggcatgatc gtgcgggtca acgacgacgg
     1261 ctcgattccg gaggacaacc cgttccacgg cgtcggcgcc gagatgggcg gcgaggccgg
     1321 cgagaacgtc cagctgacgt gggcctacgg catccgcaac agcttcggca tcgcggtcca
     1381 ccccgagacc ggcgacatct gggagaccga gaacggtgac gactcgtggg acgaggtgaa
     1441 catcttcccg gccggctcga actccggctg gtggcagctg atgggcccgc cggagcgctt
     1501 cgacgagttc cgccagatcg agaccgagtc cgaggacggc accgacaacc ccgacgtgcc
     1561 gccggaccag ctcgccgaga gcggcgacga ggcccagtcc cggctattcg cgctcgacgg
     1621 cagccagtac gagccgccga tcttctcgtg gcgctacccg ccggcggcca cggcgatcgg
     1681 cttcgtggtg gacgatgccc tcggcgagtc ctcggccaac acggcatgga tcggctcggt
     1741 gctgacggac agcatctacc gatacccgct cgccgccgac ggctcgggct tcgacttcgg
     1801 cgacgatgag gggctcgccg atggcgtgga cgacaactcg gccaagggcg accccggcga
     1861 gagcgctgac tacatgatcg gctccggctt cggcgtcgtg acgcacatcg tccaggggcc
     1921 cgacgggatg ctgtacgtga cgagcctgtc caaccgcgcc gtgtaccgca tcgggccggc
     1981 cagctgaagg aggaggcgcg ccgcaccccg ggtgcacgaa tggcacgcca ccctgccacg
     2041 tgacgtcggg ggtctcgggg tgatcgctct gtggagtgtg ggccgatcgg ccctgtcggg
     2101 gaccggtcgg cgggcgccac gctgcgcgtg gaatcgcgcc gtggccgacc gcccaccggc
     2161 cgatcgcacc ccgaggagct ggcacgtatg ctgaccccga tgcaccgtca ccgagccctt
     2221 ctgccccgga ccatggcggc attggccgcg gccctcctgc tgctcgcgtc attcgcctcg
     2281 atcgcgaccg cccagagcct gaccgaccgc gtcacggacg aagccggagt cctcgacggc
     2341 ggcggccgga gcgaggcgct gaacgcgatc gaacagctcg agtccgcggc gaacatccag
     2401 ctgtgggcgc tgttcgtcgg cagcaccggt ggcgagccga tcgacgacta cacgcagcgc
     2461 ctggtcgacg acaacgggct cggcgtgaac gacgcgctgt tcgtcgtggc ggtcgacgac
     2521 cgccgctacc agctgtggat ttccgactcg ctgaccgacc aggtcaccac cgacgagcag
     2581 gacgcgatcc tcgccgagcg cgtggagccg gcgttgcgtg acggcgactg gagcggatcc
     2641 gtcgtggcgg ccgctgaggg cctggccgat gcggcgacca cctccggtgg tggcggcggc
     2701 ggaggcggtg gtggcggcga cggcggcgat ggtggcaccg gtggcggcga ccaacccgcc
     2761 ggcgagccgt cgagcgggcc cagcttcttc gtggtgctgc tggccgtcgt cctcatcggc
     2821 gtcggcggct ggctgctctg gagccggctg caggccggtc gtgctcggga ggaggacgac
     2881 cgcgaacgag agcgacggtt gcgcgggctt tcgcagcagg ccaacgcgct cctgatcgag
     2941 accgatgagc tgctccgcca cgatgcccag gagctcggct tcgtcgaggc cgagttcgga
     3001 tccgatgccg ccgacccgtt ccgggaggcg ctggcgaagg cccgcgccga gctgcaggcc
     3061 gcgttccgcg tgcgacagca gctggacgac gaccagccgg agacgccgcc cgagcgggag
     3121 aagctgctga acgagatcgt cagccgatgc gcgagcgcga ggaagctggt cgaggagcag
     3181 accgagcgct tccgcgagct gcgcgacctc gagcgtcgcg cgccggagat cctggccgag
     3241 caggagcgga agatcgccgc cgtcgaggca cgcatcccgg acgtggagca gtcgctcgag
     3301 tccctcgcct ccgatgcgtc cggctcgtcg caggcggtgc gcgggaacgt ggccgaggcg
     3361 cgcaagcgga tcgagctggc gcgcggcggc gtcaccgagg gccaggcggc gctcgggcgc
     3421 aacgagggac cgaaggcagc tcgcgcggcc aaggcatccc aggacgcgct ggcgcaggcg
     3481 tccggactgc tcgacgccgt cgaccgcgag gccgcgacgc tcagcgaggc ccggaccgcg
     3541 ctcgacggcg ccgtggccca ggcccgcgcc gacctcgagg ctgcctcaca ggcggtcgcg
     3601 ggtgcgtccg accgcgacca ggccgacgag ctggcggccg cccaggcgaa gctgcaggcg
     3661 gccgatgccg cgctcaccgg tacgccccgc gacctcgtcc tcgcctaccg gctggcgcgc
     3721 gaggcggagt cgcaggccga gcaggtcgtc gcccgcgtcc atgagggcga gcagaagcgc
     3781 gccaaggagc tcgccgcggt cgacgccggc attcgcgccg ccgagctgtc actggaccgt
     3841 gcatccgagt tcatcgcggc acggcgtcac ggcgtgggcc ggcggccgcg gaccgcgctg
     3901 tccgaggcgg acgtcgcgct cgacgaggca cgccgcctgc gccaggccga tccggccgcg
     3961 gcgctcgcgt cggccaagcg cgcgacgcag ctcgccgacg aggcgtacac ccgggcacgc
     4021 aacgacttcg ccagcgtcga ccggcgcggc tacggcggca cggtgatcat cgacggtcag
     4081 ccctactcgt ccggtcggtc gagctggggc tccgatgtgg ggggcgccat catcggcggc
     4141 atcatcggct ccatcctgtc cggcgggggc cgcggtggag gttggggcgg tggcttcggc
     4201 ggcggaggcg gcttcggcgg cttcggcgga ggcggaggcg gcggcatcgg tcgcggcctc
     4261 ggtgggggag gacgatcctt cggcggcggc ttcggcggcg gcgggggacg ctcccgtgga
     4321 ggcggttggt aggacaatca agcgtcggcg tcgcgatcgg cgtccggcac cgcatggagg
     4381 actcgaatcc catggcacag accaccatcc tgggacggat cggccagctg gtccgcgcga
     4441 acatcaacgc gctgctcgac agcgccgagg acccggagag aatgctggac cagctcgtcc
     4501 gcgacttcac caacaacatc gccgaggccg aggaggccgt ggcgcagacc gtcgggcacc
     4561 tgcggctgct cgaggacgac ctgcgcgagg cgcagtcggc gcagaccgag tgggccgaca
     4621 aggcgcgcgc cgcggcgcgc aaggccgatg agatgcggtc cggcggcaac tcgtcggagg
     4681 cggaccggtt cacggagctg gccaagatcg ccatccggcg gcagatcagc ttcgagaacc
     4741 aggtcaagac cttccagggc cagatcgagc agcagaccca gctgaccgat cagctgaagg
     4801 acggcctcaa caagctgcgg ctgaagcgag aggagctggt ccagaagcgc gacgagctcg
     4861 tgtcgcgcgc caagatggcg tccgcccagc ggcaggtcca ggaggcggtg cgcgaggtgt
     4921 cggtcatgga cccgacctcg gagctcaacc gcttcgagga gcgcatccgc aaggaggagg
     4981 cgatggcccg cggcatggcc gaggtctccg cttcgtcgct cgaggaggag ttcgcctcgc
     5041 tcgacgacgc cgatacggag gccgaggtcg aggcgcgcct ggcaagcatg cagggcgcct
     5101 gaccgcaacc ttccatccct cgggtgaccc gcaccacggg tcatccaggc gttgagcacg
     5161 agctccggct tcgcgtgacc cgcaccacgg gtggctcgtc gtcggagctc gccggatctc
     5221 ggggttgcgg catccgttga cccgcaggac gggtcagcac gagccaaacg cgccggcgac
     5281 cccggaagcg ccggatctgg cacgctcgct gctcgggcga ccggcatggc gacacgaacc
     5341 gacgcgcatc acactggcca cacccggcgc gccgcgagac ggccgacgcg cgctccggcc
     5401 gaccgtcggg aggggtcggc gcggggactg gccgatcggt tccgggaatc ggccgagatc
     5461 atcacgcacg cggtcggcac gccgtgggcc ctgggcgtcg cggccctgat cgtggttgcc
     5521 tggggagtga ccggtccggt gttcgggttc agcaacacct ggcagctggt catcaacacc
     5581 ggcaccacca tcgtcacgtt cctgatggtg ttcgtcatcc aggccagcca gaaccgcgat
     5641 tcgcgcgcca tccacctcaa gctggacgag ttgctgcgtg cgacgaaggg cgcgcggaac
     5701 cggttgatcg acgccgaaca cgaggtggag cgcgacatgg agcgtgacga gcgcgacctg
     5761 caacgccgcg cgagatctcg acgcggctag gcctggtccg cgcggacgtt cgcgtcgccg
     5821 accccgtgga agccgaggcg ttcgtagatg cgctcggccg gctcgttgcc ggcggtgacc
     5881 cacaggtgga cgcgccgatg gcccgcctgg gccagcgagc gcatggccag gcgcacgagg
     5941 ccgtcagcca ggccccgtcc cttgtgatcg gacgccgtgc agacgtaggc gatcagcggc
     6001 gtaccctcga actcggagac caggaccgcg ctgaccagcc gctcaccgtc gtacgcgagc
     6061 cggctgtgcc cgagcatcgg ccgcccgctg ccacctcgga ggtagtggtc gatctcgccc
     6121 tgcgccacgt cgagcgtctc gctgccgtcc gcgtcgatgg tgccctggta ggcgtcgagc
     6181 atcagctcgg ccagggcggt gcgcgcggat cgctcggccg tgcgcacggt cagctgcagc
     6241 ggacgcggct ctacctcccc caggtccgcc accaggtgcc gccgcgtcac gccgcgatga
     6301 tgccaccgct ggcacgcggc gtgctccgtc gcgccatggc atcgccggtt ccggcgcagg
     6361 tccgtcaggc ggtgtgcgga tgggtcatcc gtcacttgcc agcgcggtcc gtgctcggta
     6421 gggtggtccc tacccatggc gtctgcccac gctgccgtgc atcggctccc gagcatcttc
     6481 gccgtcgtgg tgaccgccgc gctcctcctg ctggccgcca ccccggctcg ggtcgccgcg
     6541 gccgacgaat tccagccggg cagtggcgtc gtccgccagg gtcccacgcg tccgcccgcg
     6601 cagcagccgc cgggttacga cgtgtcgtac ccgcaatgcg gaacgtcgct gccggaggcg
     6661 ttcgccttcg cggtcgtcgg cgtgaacggc ggtcgcgtct tcagcccgaa cccatgcctg
     6721 ggcaccgggg agaatccgag cgagctggag tgggccgggc ccgaggcgca gctgtacgcc
     6781 aacacgggca acccgggccc cgagctctcg aagtactggc cggatggcca gaccaccccg
     6841 ctcgagtgca acaccgccgc caacccgggc ctcgacacgc ccgaatgcgc ctacgactac
     6901 ggctggaacg cggccgcgga ctcgtaccgg accgccatcg ccgcctacgt ctcgttggga
     6961 tgggccgatc ccgacgcgga ccgcacgcca gtggccaact tctggtggct cgacgtcgag
     7021 accagcaaca gctggcggtc ggagcacgcg ctcaacatcg cggtcctgca gggtgcggtg
     7081 gcgtacctgg agtccgtcga ggtcgggggc atcggcttct actcgacgcc gcagcagtgg
     7141 ggccagatca ccggcggcac ggatgtcttc gccacgtatc cggcgtggca cgccggcgcg
     7201 cgcacgctgc gcggcgcgat ggcgaactgc cgcgacgtgc ccttcaccgg tggcgcgctg
     7261 gtgatgacgc agtacctcga gcgcggactg gacgcgaact acctctgtcc ctgaccgggc
     7321 ggcctacccc gtagcgggga gctgagccgt ggcgcgcgcg ccctcagtcg aggtgcggcg
     7381 ggagcccgaa cagcggaaac cagcgcttcg tgtcgaggaa gaacgtgatc ccgacgatct
     7441 tgccgtcgcg cagctccagc acctgcagcg accacggagc gtggccgccc gccgggtcgg
     7501 gcttgtactg cgcgaacgcc ggcgagccgt tcgcggtggt cgggatgagc ttcgagccct
     7561 cgcacgcgta gccggggccg atgcaccagg ccacgatgtc ctcgtgcgtc tgcagccaca
     7621 ggtcgtacgg cggcatgttc cagacggcgt cctcggccag cagcgcggtg agcgcctgca
     7681 tgtcgtagcg ctcgaacgcg tcgacgtaac gggccagcag ctgccgcgtc tcgggatcgt
     7741 ccggcgcggc cggcggcgcc tcctccgtga ggttgcgagt gttcagcgtg tcgcgcgccc
     7801 gctggagcgc cgaattgacc gatgcgaccg aggtgtccag cagctccgcg acctccgagg
     7861 cgcgccagcg cagcacctcg cgcaggatga gcaccgcgcg ctgcttcggc ggcaggtgct
     7921 ggagcgcggc catgaacgcc agccgcaccg attcgcgcgt ggcggccacc tccgccggat
     7981 cgccgtcggc cgagaccacc ttcgcgtccg ggattggctc cagccagaga ttctcctcca
     8041 gcggctgcgg gagtggcgtc gaggccgact gcgcgggacg caggtccatc ggtcgcgcgc
     8101 gtcgctcgcg acccttgagc atgtccaggc agacgttggt cgtgatgcgg tacagccacg
     8161 agcgcagctg cgcccgccct tcgaagcggt cgaggccgcg ccaggcgcgc accatggtct
     8221 cctgcaccgc atcctcggcc tcgaacgagg acccgagcat gcgataggcg taggcggtga
     8281 gctccgtgcg gtgctgctcc agctggccgg tgagctcggt cgcgtcgggt gcgagctgcg
     8341 tcatggcgga catcctatcg gtcgccacgg gatccgtcct cccccagagc ggcggaggcc
     8401 gccgcccgcc gcgacagcgc ggccagtgcc atggaccagc ggctgacggg cagcagctcc
     8461 cggtgccacg cggcgtcggt acggagacga cggcggacgg cgaggagctg cacgccgccc
     8521 ggatccgcgc tgcgcagtga ggccagtggg agccagccga gcgccagcag gcttcccggc
     8581 ccgaagcgcg ccgatgcgcc gccgacgcac cgcacctcca gcgtgccggc ctccaccagc
     8641 acgaccgcat ccgcccacgc gtccggcgtg ctggcccggg acggtcccgg cgggatggag
     8701 acgcgccagc gctcgaagcg cccccgcgac attcgcgtcg ctcaggcttc cggcgcgatg
     8761 ttctgattgc gtcggaagac gttgtccgga tcgtagcggc gcttgacctc gaccaggcgg
     8821 ttccaggtcg tgccggggta agcggcgcgc acgcgctccg gaccttcgtc ggccaggaag
     8881 ttcacgtacg cacccgcatc cgattggcgg agggcatcgg ataggtcggc gacccaggct
     8941 tcgcgccgcg aacggtcccc ctcgccatcg tagaacgaga agacgttcac catgatgcgg
     9001 ctggcccgat gcgcgtaggc cgtcgcgtcg gacggaacgc gggccgcggc gccgcccagc
     9061 acgcgcagct gagcgccgcg catcggcgcg tcggaggcct cgatggccgc gacgatcgcc
     9121 gccgcgtcct ccagcccgat gcgctcgacg aacatcgttc gcgacacggc ggtcgggtgg
     9181 tagtccgggt cttccggcgg atacatctcg ggataccgga tcgggcggac catgtccgcc
     9241 agcggggtag cgagcgcgcg gaagggggcg taggcggcat cggcctcctc gacggcgccg
     9301 gaccagcaca tgagcgccat gatgaccggc gttccgtggt gctcctccgc cacgaacggc
     9361 atcggtgggc aggccatcac gttggcgatc gccgacagct cgtcggccgc cgcatcggcc
     9421 gcggtcatga aggcggcgat ggtgtccggc gtggccggca ggatgagcat cccgcccacg
     9481 acctggtcga gcgggtgcag gcggtaccgg aaacgcgtga cgacgccgaa gttcccgcct
     9541 ccgccgcgca gcgcccagaa caggtccgga tcgcggtcca cgtcgaccgt gcggatctcg
     9601 ccatcagcgg tgacgatctc cgccgccagc acggagtcga tggtcatgcc gtgcttgcgc
     9661 accaggtagc cgatgccgcc gccgagggtg atgccgccga tccccaccgt cgcggtgtcg
     9721 ccgaagccga tggcgaggcc gtgcgccgcg gccgcctcgg tgacctcgcc cgccgtgagg
     9781 ccggcatcgg cccaggcggt gcccgtgtcg gtgtcgaggt cgaagcgacg caggtcgcgc
     9841 aggtcgatga cgatcccgcc gtccgccgtg ccgtgtccgg ctcccgagtg accgccgctg
     9901 cggaccgcca ggtcggcgcg gatgcgccgg gcgtgcgcga tggtgcgcgc cacgtcgtcg
     9961 gtgcccgcca cgcggacgat ggccgcggga cgggggtcgg tgccccccag cgcgatccgg
    10021 cgcgtcgcgt cgtagtcggc gtcgtccggg gtgatgaccc ggccgcggag gccggcgcgc
    10081 agctcgtcga tggttcgggt cagggtcacg ggtggtcgct cctccggtgc gttcgtccca
    10141 tctgacgacg gccgatggcg gaactcatcg gccgcccggg tcagcggtgg ggaagcagct
    10201 cgaagcgctc gacgtggcgc ggccgcacga tctcgaagtg gcgccgatcg agggtgtaga
    10261 tccggaccgc gcccatccgt tcggcgatcg tcgccacggc ggcatccgcg aagccgatcc
    10321 cgtgatcggc agcctccgac atgatcgaca ggacgcgcgg gatgtcggag ccggccagcg
    10381 gctcgagcct ccagtccgag tcggcgagat agcggatgaa cgccgtctcc cggctggacc
    10441 cgagccgcga gccgatgagg gtgcacacct cgggcagggt cggcatggga acgacgatcg
    10501 tctcgcgctc gtcggacacc gccgccacgc actcgtcgtg cagcgagtcg ccggcgtcga
    10561 ccagggcgta caggacgccg ctgtcgatga ccgcggtcac cgtgcggggt cttcgaggtc
    10621 ggcgggcagc agctcctcga tgcgctccgc gacgtcacca gggccgcggc cgatgccgac
    10681 gaagccgggg agctggcgct caccgtcggt ctgctcggcc acgtacgcct cgagcgcgcc
    10741 gcgaatgacg tgcgcggtcg gcacgccctt gcgccgcgcc aggccctcga gctcggccaa
    10801 aagtccgggg tccagcagga ttgacgttcg cttcactcgc cgatactatc acgcacaagc
    10861 gaatatgcat atgcatatgc ctatatcggc gcctatgcgt cttcgcgggg gatagggttt
    10921 ccgcttcgct ccggtctgct cgccccactt gttcacacaa ccgcagcagc ccggagaact
    10981 gccctcccgt gcctcacgtc accctcaccg cgcgtgctca ccgcgcgctg ttcggcgtcg
    11041 ccatcaccct cacgatcgcc gtccagtccg ccgtggcccc ggccgccacc ttcggcgcgg
    11101 atgccgccgc gcagctcccg tcgcccgttc ccgtcggcga cacccacccg tcgctcgagg
    11161 cgcaactggc cggcgcgctg cgtcgcgctc gcgagaacag caactcgccg gggctgagcg
    11221 tcgccgccat ctcgccgtcc gggaagacct ggtcgggcgc caatggcacg ttccagagcg
    11281 gcgccagcct caacccgacc gatgcgctca ccatcggcag cgtgacgaag accttcgtgg
    11341 ccgccgttgt cctggcgctg gtcgacgaag ggcggatcca gctcgacgtg ccggcctcca
    11401 cctacctgcc gcgcgcgatg gcgctggaga acggggcgac cgtccggcag ctgctgagcc
    11461 ataccagcgg catcgccgac ctgtacggac cggccaagga cgtgctcagc ggcgacccgt
    11521 ggcgggtgat gggctcgaac gacgtgctcg agccgatcgg cggcgactac ttcgcgccgg
    11581 gcaccggcta cgcgtactcc aacacgaact actacctgct cggccacatc gtggaggtgg
    11641 tgacgcacca ctccttcaac gacgagctgg cggcccgctt cacgggaccc ctgggcctgg
    11701 ccagcgtccg cctgctcacc gccgcggaca ccgtcatgcc ggccgcatgg accacgacct
    11761 tctggacgtc gggcgccatg gtgtcgacgc cgatggacct cgcgcgctgg gggcgggccc
    11821 tgtacggcgg acaggtcctg tcggaggcgt cgaccggccg gatgctcgac ttcagcgccg
    11881 gccaccacta cggcctgggg gcgcagctcc tgccgctcgg tggccaggag gtgccagggc
    11941 actcggggct gatgtacgag atgacgacgc tgctggtcag gctgccggac gggatgacac
    12001 tggccatcgt cagcacccag ccctacacgg acctcgaggc ggtgctggtc gggaactacg
    12061 ggggcccttc gctgctggag ctgctctacc acctcgacgg ctgagcgctg ctatcgtcgc
    12121 ccgatgccga cggtctcgcg cctctcgatc acgccggtcc gcagcctggc gctccaccat
    12181 cccgacgccg tcgagctcgg gccgcacggc gtcgcggatg accggcgcta cctcgtcctg
    12241 acgccggacg accggctgtt cgacggcacc aagcacggac cgctggtgcg catgcgggcc
    12301 gatctggctc acgatcccga acggctcacg gtccacctgc ccgacggccg cgtcgtcgag
    12361 gccgtcgtgg agctcggagc ggcgcggcag gtgacgatct acggtcgcac cttcaccgtg
    12421 cgggccgtga tcggtccgtg ggccacggcg atctcgggtg tcatcgggcg tgcggtagcc
    12481 ctggcgcggg tcgagcggct tcccgaggag cgagaccgca acccggtgtc gatcgtttcg
    12541 gaggcatcgg tccacgagct ggagcggcag ggaaacgacg gccggccgct cgacccgcgg
    12601 cggttccgca tgctgatcca gctcgccgat gcggacccgc accaggagga tggctggatc
    12661 ggccggcggg tgcgtatcgg ctccgaggtg gtcgtccggg tgaccaggcc ggacccgcgt
    12721 tgcgtcatca cgacccagga tccggccacg ggcgtgcggg acttcccgac gctgcacgcc
    12781 atcaaggcct accgcggcct gcgagacggt cgccacatcg acttcggcgt ctacgcggac
    12841 gtggaggcgc cgggtgtggt gcgggtgggg gacgacgtct cgcccctgga ctgatcggtg
    12901 cggccctctc tcggcacgcg ctgtgcgtgc gtgtcggtca aggaggtgca ccgatgccgt
    12961 acgacaggat ctcggatctg cccgactccc aggtcgacca gtacagccac caccagaagg
    13021 aggcgttcct cgcggcgttc aacaacgccc tgaaggagta cgacggtgac gagtcgcggg
    13081 ccttcgccac cgcccacgcc gcggccaagc ggacgccgaa ggaggatccg acgccggggg
    13141 actgagagtt tccgggagag ctccgcatcc ttgggggttg cctgcgggtg gtggttgcgt
    13201 ggggcgcatc tatccgcgcg gaaggttcct cgtcgcggcg tgcgggtgat gtgggtcgcg
    13261 cgtggcggga ctgccgagga cgatcgcgcg tgcgggccgt caatagttgc ctgtggcgcg
    13321 acttgccggg acgatctcgc ctgcggaccg tcaatagctg cctgtggcgc gacttgccgg
    13381 gatggcaagt aacgcccgag aagcgggttc cgacagcgag gcgccggcgc tggtttgcac
    13441 ccctcgcaag cccgcgccaa tctcgggccc tatctcggga attacttgcg cccagcgcaa
    13501 gcggacggac cctccgaccg gttgcgtgcg gcgcgtaggc tgatcgctga tcaccacctc
    13561 agccacggga gcgatcgacc gatgtccacc gagcagtccg gtccccccag cgagcccgag
    13621 ggcgagcgcc ccaccacgcc gtccccgacg ccgacgcagc tgcgcctcca gatcctgtcg
    13681 acggagcact ggagcctgct cgcgtcacgt gggctggcgt ggaacgagtc gttcacccgc
    13741 gcgagcatgt tcctctcgac gctgtcgttc gccgtcgtgg cactcgcgct ggtggggcag
    13801 gcctccggct tcgggcagga gttccggctc ttcgccctcg tcatcctgcc ggtcgtgctg
    13861 ctcctgggca tggcgacgtc ggtgcgcatg gacagcgcca accaccacga cgtgcagtgc
    13921 gtgatcggca tgaaccggat ccgcgccgcc tacctggaga tcgcgccgga cctcgagcgc
    13981 tacttcgtga tgggcacgac cgacgacatg gccggcatcg tccggaccat ggcggtcctg
    14041 ccggaccgat cgacccgcct caacctgctc tcggcgagcc cgacggtcat tgccgtcctc
    14101 aactcggtgt tggccggcgg gatcatcggc ctcgcggtga tgcagttcgg gatggcgggg
    14161 gacgccgccg tcatcgccgg ggtgctcggg ttcatcggcg tcgcgacggc gtgggcgtgg
    14221 tacagccgca atctgctgcg ccacctggtc cacgactacg agccgatgtt ccccggtgcg
    14281 gaccccggga cgccgctgcg ggtctgaagc cggcctagac gcgcgctccc gaccggtcgg
    14341 gcggctccgg gcgctcgagg cgctcgtcca gtccctcgac caccacgccg ttgagcgccg
    14401 cccacggtcg gtggtagcgc tcctcgatct gcgcgggcgg cggtgggcgg ttggtgagcg
    14461 cgtgctcgag cccggcgagc agcccgccga tcagcgctcc ggccgatgac cgcgacggca
    14521 gccggtcagg ccgtcgttcg tccacgcgcg gccttcgcct tccgggcggc cggcttggtc
    14581 ggtggcgtag aggagccccc ggcgatcgcc tcgtccacgg cgcgccgggc gaggaagcgc
    14641 gatcggtacg agaggggaga gggtgacgca tcggctgcgc ggtagcgcca tccggtcagg
    14701 cctggctcgt agaagatctc gtagccggcg tactcctcgg cggcgtgggc gaccggcttg
    14761 gtggtggacg tccggcggga ggtggcggcg gcgcgacagc gggcgcagcg gttgaccgcc
    14821 aggccgtgcg ggcagtctcg acacgacgcg cactggtccg cgggaaagcc atggatgcag
    14881 ggtgtgagca cggtgggcct ctggggtggg tacccagcgt actccgatcg cgatcggcgc
    14941 ggatcccgag cactcactcg acgtgtggta ccatttcgaa aacgtgttcg attggcgctc
    15001 cggtctgcgc cgcgggacgc ttcgacgtca ccgccccgat ccacgaccca acctcgtgcc
    15061 ctgaccgggc cccgcgatgc cgcgggtggg ccccaccagg agccattctc ccttgtcctt
    15121 tgcgaatctc gggctgacgc ccgacctgct ccgtgccgtc caggcgcagg gctacaccga
    15181 tccgaccccc gtccagcgcg aggcgattcc gctggtcctc cagggtcggg acctcctcgc
    15241 cggcgcgcag accggcaccg gcaagaccgc cgccttcgtc ctccccatgc tccagcgcct
    15301 cgcggccacc gagcgacggg cgaacgacgg acggccgcgc atccgcgcgc tgatcctcac
    15361 gccgacccgc gagctcgcca tccaggtcga gcagtccgtg cgggactacg gcgccggggc
    15421 tcgacgctcg acgaccatct tcggcggcgt ggggatgccc ggccaggtgg ccgcgctgcg
    15481 tcgcggtgtc gacatcgtgg tggccacccc cggccgcctg ctcgaccatg cacgccagcg
    15541 gaccatcgac ctgcgcagcg tcgagatcct cgtcctggac gaggccgacc ggatgctgga
    15601 catgggcttc atccgcgaca tccgctccgt gctggcgctc ctcccggacc gccgccagag
    15661 cctcctcttc agcgccacct tctccaacga gatccgcagc ctggcggaag ggatgctcga
    15721 ccgaccggcg tcggtgcagg tcaccccgcg caacacgccg accgagctgg tgcggcaggt
    15781 ggtccacccg gtggaccgat cgcgcaagcg ggagctgctg agccacctcg tccgcaccca
    15841 cgcgatcgag caggccctgg tcttcacccg caccaagcac ggcgcgaaca agctcgccga
    15901 gcagctgtgg aaggacggga tcgcgaccgc cgcgatccac ggcaacaagt cacagccgca
    15961 gcgcgtgcgg gccctcaacg acttcaaggc cgggcgcgtc acgctgttga tcgcgaccga
    16021 ggtggccgcc cggggcctcg acatcgagca gctgccgcac gtcgtgaact acgagctgcc
    16081 gatggtgccg gcggactacg tccaccgcat cgggcgcacc ggccgtgccg gcgctgaggg
    16141 cgacgccatc tcactggtgt gcgtcgacga gcggccgctg ctgcgcgaca tcgagcgtct
    16201 gctgggcgag ccgatcgcga ccgaggtcat cgcgggcttc gagccggatc cgaccgcggt
    16261 cgccgagccg atccgcctgc ggagcggcca gcacgctggt cgggcttcgg caccacgcca
    16321 ccgagccgca ccgcgcgccc acgcgggggc accgtccggc ggttccgcgc cacgtcgtcg
    16381 cggcggtccg cggcgcgccg gcgcgagcat cggcaaccgc gagcgcggct ggagcagcct
    16441 gcccggcgag cgacgccgag ggtaggcggc ggccggtggt cacccgggtc atcctcaccg
    16501 gcgacaccca cctgccgcgc ttcggccgct ccctgcccgc cgcgttggtg gaggcactga
    16561 ccgacgccga cctcgtcctg catgcgggcg acctgaccga tccgttcgtg ctcgacctgc
    16621 tggccgagca caccccggtg gtggccgtgg ccggcaacaa cgacgcgccg gcgctgcacg
    16681 agcgcctggg gctgacccgg gtcgtggagg tcgagggcgt gcgcatcggt ctgacgcacg
    16741 ggcacgccgg acgcggccgc accacgccgg atcgggcacg caacgccttc gacgaccagg
    16801 gcctcgacgc gatcgccttc ggccactccc accagcccgt cgtcgagcgc gtcgacggtc
    16861 ggtggctgct caatcccggc tcgccgaccg atcggcgccg ccagccgacg ttcagcttcc
    16921 tgcgtctcga ggtcgagggc cgcgatctcc ggcccgagct ggtcacctac gcccaccgtc
    16981 gctgaccgcg cacgagattc acaacctctg gcactggccg gcgcccctgg acggcaggat
    17041 cgtgatcgtc tgatcgcgcg cgtggaggca atcggtcatg cgacacgtgg aaacgacgac
    17101 cagggtgccc ggctcgccgg agcgggcgtt cgacctcgcg gtcgatccgc atcggatgag
    17161 cgagcagatg ccctgggtgg gcggcatcac cgaggttcgc gggcgcggcg atcgactggg
    17221 cgactcgttc gtgttccgcg atcacgtgtt cgggcgctcc gagcgggcga tgacggtcgt
    17281 gaccgcctgc catcgaccga ccttccagac gaccgagacg acctacgaca acggcttccg
    17341 cgtcatctgg accatgcact tctcgccgtg cgaggagggg accgagatcc gcaacgtggt
    17401 gtggtgggcg aagccggacg actgggtcgg ccgcgtggag gagctgat
//
LOCUS       DASTYP010000091         9424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1062914, whole genome shotgun sequence.
ACCESSION   DASTYP010000091 DASTYP010000000
VERSION     DASTYP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062914"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFV59_04385"
     CDS             complement(<1..372)
                     /locus_tag="VFV59_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEU5325101.1"
                     /translation="MITRTSVYERVGGMAFFERLVDRFYAGVADDPPLRELYPEEDLA
                     GARHRLTLFLAQYWGGPRTYDQERGHPRLRMRHVPFAIDADQRDRWLRHMRAAITDLA
                     PSPDVAAELERYFLMAAESMRN"
     gene            479..1417
                     /locus_tag="VFV59_04390"
     CDS             479..1417
                     /locus_tag="VFV59_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325102.1"
                     /translation="MHSAPDNAAVISARGLTKHYGSVAALNDLTLDVRPGEIFGFLGP
                     NGAGKSTLIRTLLGFLHPTRGAATVLGHDVATESVEIRRLTGYLPGGVALYDSLTGNQ
                     VLDYLASLQGRGSHRRAELVDRLQMPASDLRRKVRDYSRGMRQKIGVIQALQHDPEVA
                     ILDEPTEGLDPLMQQSFYGILADLRAEGRTIFFSSHILSEVERVCDRVAIIRHGNLMA
                     VHEVRELLARRRRRVSIQWRGAAPDPATLPGLADAVVEGDRLFGTLHGDVAEFVRAIA
                     SPSLQDLTIEPASLEEAFLEYYADDVPARDVEEPAP"
     gene            1414..2217
                     /locus_tag="VFV59_04395"
     CDS             1414..2217
                     /locus_tag="VFV59_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5325103.1"
                     /translation="MNGSLFARTVAWQRARLIVVVLAAFGWGVLIPIFYTGFSDVVRD
                     LANSGAFPEELLSFGSGSLFSLPGAVTLGLQHPFAISLLGIFAAGATATAIAGERQRG
                     TLEVLLARPLSRRSVYVSIGLALLLAVALVLAALLGGFVTGAATQGVLDDLSVEHLPM
                     VFLNGFLLWGAFATFGMAASVSFDRTGPAIGLSLAYLLVNYFFEILGSLWTDAEWTQE
                     YSLFHHWQPAEILQGALDPIDLVIVGVAFLLPIVYALVVFPRRDLAAPS"
     gene            complement(2230..4917)
                     /locus_tag="VFV59_04400"
     CDS             complement(2230..4917)
                     /locus_tag="VFV59_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5325104.1"
                     /translation="MPQLPTGTVTFLFTDIEGSTRLAQDRAADWPRLLGRHRELIREA
                     VARHGGVEVSTEGDSFFVAFDSATGGLAAAADAQRALAAEPWPEDAEVRVRMGLHTGE
                     AEVRDGTYVGVAVHRAARIGSAGHGGQVLISDSTRALVVESLPPGIGLLALGRHRLKD
                     LEGDEGLTQLVVDDLPTEFPPLRTLDATPNNLPTQVTSFCGREDEVREVTELLAQVRL
                     LTLTGPAGTGKTRLALRVAAGVMDRFPDGVFFVPLGSISEVELVIPTIAHEVGLPDRG
                     GSHPMERLTAHLRDRTTLLVLDNFEQVLGAAPLVGELLSGVASLSVLATSRSPLHVYA
                     EHEYGVPPLDVPDLRHLPALDKLSQYESVALFIDRARAAVPTFMVRNENAAAIAEICY
                     RLDGLPLAIELAAARVKLLTPQAILARLAQSTTLLTGGARDLPARQQTLRGAIDWSYD
                     LLDEGDRRLFAGFSIFVGGAGLEAVEAVCDDPDLDVLDHLASLVDKSLARRGETADGE
                     PRFSMLETIRDYALEKLRDSGRHDEIAERHATWYLDLVERAADAVMGADKRRWLDTLD
                     VEHENLRGAVSWAIETGHAETAMRFVSALWRFWQMRGYLKEGLERTRAALAMPGAADV
                     PALRARALDAAGGLAYWIGDGDAALDYYEQELALHRELGDRAGEAWTLYSLSFTWTYT
                     KSRRLGSGKLDKERLERAHEIVKEAVRIYDEIGDRSGQAQALWAQSNVLWALDPETTD
                     EAFHEAQRTAHDALQLFREMGDSFMIGWTCYTLALTAIRLHELDQARALLAESIRIFD
                     EAGDVTGYVLVLDGFAAVAFHDEDWERAARLSGAVSTLERRTGTGLAPPNRLLLRFDP
                     DRLKDEPATAAVWAEGERMTIPEAVAYALEGADAPRREG"
     gene            5030..5956
                     /locus_tag="VFV59_04405"
     CDS             5030..5956
                     /locus_tag="VFV59_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5325105.1"
                     /translation="MRSLPAALGRRPVLAGVLGALTIAFSAIFVRLADVEPATAAIFR
                     CFYALPLLAVLAWNERRRYGPRAPGQARLAWIAGVFFALDLVLWHHSIEEVGAGLGTV
                     LGNTQVVVVPLAAWLLLGERPGARVAASVPVVLIGVVLISGVLDGGGAYGRNPLLGVL
                     FGIGTDIAYAGFLLVQRRANADHRRPAGPLFDATLSAAIMSLLIGLPLGEVDLVPTWP
                     AHGWLILLGVAVQVVGWLLISISLPRLPAAITSVVLTIQPVGSVLLGVVLLAEAPSAF
                     QLVGVLFILAGLLLTTLRVRPHRWRRAQPEVG"
     gene            complement(5953..7302)
                     /locus_tag="VFV59_04410"
     CDS             complement(5953..7302)
                     /locus_tag="VFV59_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5325106.1"
                     /translation="MSDQPHQHLPISDIAERLRATPLFGGLADDQLERLTRMGEIVDL
                     APGDRLINEGDVADALYVVLDGELDVTKRAGTSEIPLARVGPGALQGEIGALEGGRRL
                     ASVMAVGEAEVLRIPIEAMRELLSAGPDVALAVIRTAVERLRGMEAGLREREKLAGLG
                     TIAAGLAHELNNPAAAAMRAVRSLGEAIATAESLPRPLPPPRPPADARPPVGALERGD
                     RIEAVAELVGYEAAQALVDDGWTPESLAAQPAEVRPWIAADASVHQLLAELSMALGRI
                     SEIVAAVKGYAYLDQAPVQRVDVRTGIQQTLVILRHRLRDGIDVRTELAEDLPEIEAH
                     GSELNQVWTNLVDNAIDAMGGRGSLTIRAEPDPDGDGVLVCVCDSGPGIPAEVRARLF
                     EPFYTTKPPGKGTGLGLHISHTVVAQHGGWIDVSSEPGRTCFEVHLPLHASTAPDPA"
     gene            complement(7310..8956)
                     /locus_tag="VFV59_04415"
     CDS             complement(7310..8956)
                     /locus_tag="VFV59_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5325107.1"
                     /translation="MAALPAILTVDDDEPVLRSVERDLRARYGDRYRVLAAGSGPEAL
                     ELSREVTRRGDVIALFVVDQRMPVMTGIELLREALPLQPDAKRILLTAYADTEVAIDA
                     INEIRLDQYILKPWEPPDDRLYPVLDDLLADWNAAFRPPFEGLRLLSDRWAPRGHALR
                     DFLTRNQLPYAWVDPTEEDGRRLAATLDPPLAPGEPVVLLPGGAVLRDPSTRDLANAV
                     GLSTHAALPFYDLVIVGSGPAGLAAAVYGASEGLKTLLVEREAPGGQAGTTSRIENYL
                     GFPAGLTGSDLARRALAQARRLGAELLSSQEVASIRRGDPYRTVVLEDGAELSCQAVI
                     VATGVSYRQLEVPGAAELAGAGVYYGAATTEAILYRDTDVAVIGSGNSAGQAAVHLSR
                     FAGRVHLIVRGDDLAATMSAYLVEQIGTIGTIEVHTRTDTRAFHGTDHLERATFAAPD
                     GELEMPLAAAFVFIGQQPRTAWLDGVVARDEKGFILTGSDVGAAQGWNLDREPYLLES
                     SLPGLFAAGDVRHRSIKRIASAVGEGAMAVQFVHQYLADL"
     gene            8995..9141
                     /locus_tag="VFV59_04420"
     CDS             8995..9141
                     /locus_tag="VFV59_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEU5325108.1"
                     /translation="MLWTIVVILLLLWFLGFLLRAFGGLIHLLLIAALVIVIYRLLTG
                     RKLL"
     gene            9146..>9424
                     /locus_tag="VFV59_04425"
     CDS             9146..>9424
                     /locus_tag="VFV59_04425"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861524.1"
                     /note="catalyzes the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU5325109.1"
                     /translation="MLLTGTGRLAPIGLSPWAIRIDDERIAWIGAPHEADDGERIDLG
                     NALVTPGLVDSHTHPVFAGDRSDEAAARLEGARYTDGGILRTMRATRGA"
BASE COUNT         1225 a         3434 c         3361 g         1404 t
ORIGIN      
        1 gttgcgcatc gactcggccg ccatcaggaa gtagcgctcg agctcggcgg ccacgtcagg
       61 cgatggcgcg agatcggtga tggccgcccg catgtggcgc agccagcggt cgcgctggtc
      121 ggcgtcgatg gcgaacggca cgtggcgcat ccgaagccgg ggatggccgc gctcctggtc
      181 gtaggtccgc ggcccgcccc agtactgcgc caggaacagc gtcagccgat gccgggcacc
      241 ggccaggtcc tcctccgggt acagctcgcg cagtggcggg tcgtccgcca cgcccgcgta
      301 gaaccggtcg accaggcgct cgaagaacgc catcccgccc acgcgctcgt agaccgaggt
      361 cctcgtgatc acgggcgcaa gtctagggag tcggttccgg gatcgcccgc ggcgcggatc
      421 ggggcgatcg gtgcgtggag gcaacttcga caccgcgcga cggctacgat cggcgtcgat
      481 gcactccgcg cccgacaacg ccgccgtcat cagcgcccgt ggactgacca agcactacgg
      541 gtcggtcgcc gcgctcaacg acctgaccct cgacgtgcgg cccggcgaga tcttcggctt
      601 cctgggtccg aacggcgccg gcaagtcgac gctgatccgg acgctcctgg ggttcctcca
      661 cccgacccga ggcgcggcga cggtgctggg tcacgacgtc gccaccgagt cggtcgagat
      721 ccggcggctc acgggctacc tccccggcgg cgtggcgctc tacgactcgc tgaccggcaa
      781 ccaggtgctc gactacctgg ccagcctcca gggccgcggg tcccatcggc gggcggagct
      841 ggtggatcgg ctgcagatgc cggcgtcgga cctgcgccgg aaggtccgcg actactcgcg
      901 cggcatgcgc cagaagatcg gagtcatcca ggcgctgcag cacgatccgg aggtcgccat
      961 cctggacgag ccgaccgaag ggctcgaccc gctgatgcag cagtcgttct acggcatcct
     1021 ggccgacctg cgcgccgagg ggcgcacgat cttcttctcg agccacatcc tctccgaggt
     1081 ggagcgcgtg tgcgaccgcg tggccatcat ccgccacggc aacctgatgg cggtccacga
     1141 ggtccgcgag ctgcttgccc ggcgtcggcg gcgcgtctcg atccagtggc gcggcgccgc
     1201 ccccgacccg gcgaccctgc ccgggctggc cgacgcggtc gtcgagggcg accgcctgtt
     1261 cgggacgctc cacggcgacg tcgccgagtt cgtgcgcgcg atcgcctcgc catcgctgca
     1321 ggacctcacc atcgagcccg cgagcctcga ggaggcgttc ctcgagtact acgccgacga
     1381 cgtgcccgcg cgcgacgtcg aggaacccgc gccgtgaacg gcagcctctt cgcgcggacg
     1441 gtggcgtggc agcgagctcg gctgatcgtc gtggtgctgg cggctttcgg gtggggcgtg
     1501 ctgatcccga tcttctacac cggcttctcc gacgtggtgc gagacctcgc caactccggc
     1561 gcgttccccg aggagctgct cagcttcgga tcgggttcgc tcttcagcct tccgggcgcc
     1621 gtcacgcttg gcctgcagca ccccttcgcc atctccctgt tgggcatctt cgccgcgggt
     1681 gccaccgcca ccgcgatcgc cggggagcgg cagcgcggca cgctcgaggt gctgctggcg
     1741 cgtccgctga gccggcggtc ggtctacgtc agcattggtc tggcgctgct cctggcggtg
     1801 gccctcgtgc tcgcggcgct ccttggcggc ttcgtcacgg gagccgcgac acagggcgtc
     1861 ctggacgacc tgtcggtcga gcacctgccg atggtgttcc tcaatggatt cctgctgtgg
     1921 ggggcgttcg ccaccttcgg gatggccgca tcggtcagct tcgatcggac ggggccggcc
     1981 atcggtctga gcctggccta cctgctggtg aactacttct tcgaaatcct cggctcgctg
     2041 tggaccgatg cggagtggac gcaggagtac tccctcttcc accactggca gccggcggag
     2101 atcctccagg gggcgctcga tccgatcgac ctcgtcatcg tcggcgtcgc ctttctgctg
     2161 ccgatcgtct acgcgctggt cgtcttcccg cgacgcgacc tcgcggcccc cagctgagcg
     2221 gggctcggct cagccctcac ggcgaggggc gtccgcgcct tcgagcgcgt acgccacggc
     2281 ctccgggatc gtcatccgct ctccctcggc ccacaccgcg gccgtggccg gctcgtcctt
     2341 gagccggtcc ggatcgaagc gcagcaggag gcggttcggc ggggcgaggc cggtgcccgt
     2401 cctccgttcc agcgtgctga ccgcgcccga cagccgtgcg gcgcgctccc agtcctcatc
     2461 gtggaaggcg accgcggcga acccgtccag gacgagcacg tagccggtca cgtcgccggc
     2521 ctcgtcgaag atgcggatgg actcagccag cagcgcccgg gcctgatcca gctcgtggag
     2581 gcggatcgcc gtcagggcca gcgtgtagca ggtccagccg atcatgaagc tgtcgcccat
     2641 ctccctgaac agctgcaggg cgtcgtgcgc ggtgcgctgc gcctcgtgga aggcctcgtc
     2701 cgtcgtttcg ggatcaagcg cccacagcac gttcgactgc gcccacagtg cctgtgcctg
     2761 ccccgatcgg tcgccgatct cgtcgtagat gcgcaccgcc tccttgacga tctcgtgggc
     2821 ccgctcgagg cgctccttgt cgagctttcc ggagcccagc cgccgggact tggtgtaggt
     2881 ccatgtgaag ctcaggctgt acagcgtcca ggcctcgccg gcgcgatcgc ccagctcccg
     2941 gtggagggcc agctcctgct cgtagtagtc gagcgcggcg tcaccatcgc cgatccagta
     3001 ggccagccca ccggcggcgt cgagggcgcg cgcgcggagc gcggggacgt ccgcggcgcc
     3061 gggcatcgcc agcgcggccc ttgtgcgctc gagaccctcc ttgaggtagc cgcgcatctg
     3121 ccagaagcgc cagagcgcgc tgacgaagcg catggccgtc tcggcgtgac ccgtctcgat
     3181 cgcccacgac accgcgccgc gcaggttctc gtgctcgacg tccagggtat cgagccagcg
     3241 acgcttgtcc gcgcccatga cggcatcggc ggcgcgctcg accaggtcga ggtaccaggt
     3301 cgcgtggcgt tccgcgatct cgtcgtgccg cccgctgtcg cgcagcttct ccagcgcgta
     3361 gtcacgaatc gtctcgagca tgctgaagcg gggctccccg tcagccgtct cgccgcggcg
     3421 cgccagggac ttgtcgacca gcgaggcgag gtggtccagc acgtcgaggt cggggtcgtc
     3481 gcagacggcc tccaccgcct cgaggccggc gccgccgacg aagatcgaga agccggcgaa
     3541 gagcctgcgg tcgccctcgt ccagcaggtc gtagctccag tcgatggcgc cgcgcagggt
     3601 ctgctggcgc gccggcaggt cgcgtgcgcc accggtcagc agcgtcgtcg actgggccag
     3661 gcgcgccagg atggcctgcg gcgtgaggag cttcacgcgt gccgcggcga gctcgatggc
     3721 cagcggcagg ccgtcgaggc ggtagcagat ctcggcgatg gcggcggcgt tctcgttgcg
     3781 caccatgaac gtcggcacgg cggcgcgggc acgatcgatg aacagcgcca ccgactcgta
     3841 ctggctgagc ttgtcgagcg ccggaaggtg gcgcagatcg ggaacgtcga gcggcggcac
     3901 gccgtactca tgttccgcgt agacgtgcag cgggctgcgg ctggtggcga gcacgctgag
     3961 gctcgcgacg cccgacagca gctccccgac cagtggcgca gccccgagga cctgctcgaa
     4021 gttgtcgagg acgagcaggg tggttcggtc gcgcaggtgg gccgtcaggc gctccatcgg
     4081 gtgcgagccg ccccgatcgg gcaggccgac ctcgtgggcg atggtgggga tcaccagctc
     4141 gacctccgag atggagccga gcggcacgaa gaagacgccg tccgggaacc gatccatcac
     4201 gcccgccgcg acgcgcagcg ccagccgcgt cttcccggtg ccggcggggc ctgtcagcgt
     4261 caggagccgc acttgggcca ggagctcggt cacctcgcgg acctcgtcct cgcggccgca
     4321 gaagctggtc acctgggtgg gcaggttgtt gggcgtcgcg tcaagcgtgc gcagcggcgg
     4381 gaactccgtc gggaggtcgt cgaccaccag ctgcgtcagc ccttcgtcgc cctccaggtc
     4441 cttcagccga tgacggccga gggccaacag cccgatgccg ggcggcaggc tctccaccac
     4501 cagggcgcgc gtcgagtccg agatcaggac ctggccgccg tggccggccg agccgatccg
     4561 cgccgcgcgg tggacggcga ccccgacgta ggtgccgtcg cgcacctcgg cctcgcccgt
     4621 gtggaggccc atccgcacgc ggacctcggc gtcctccggc cagggctcgg ccgccaacgc
     4681 gcgctgcgca tcggccgcgg cggcgagccc accggtggcg gagtcgaagg ccacgaagaa
     4741 cgagtcacct tcggtgctca cctcgacgcc gccgtgcctg gcgacggctt cgcggatcag
     4801 ctcacgatgc cgtccgagca gccggggcca gtccgcggcg cggtcctggg cgaggcgagt
     4861 cgacccctcg atatcggtga agaggaacgt gacggtgccg gtcggaagct ggggcatcgg
     4921 agtgcggaag tgtgacggcc aagcggtcgc cagcgcaaga cctccgcgac gacctcgcaa
     4981 cacgtcgcga gatcagcggg agcgccgttc ccccgctacg ctgcaccaga tgcgctcgct
     5041 ccccgcggcg ctcggccgtc gcccggtgct ggcgggcgtg ctgggcgcgc tgaccatcgc
     5101 cttcagcgcg atcttcgtgc gcctcgcgga cgtggagccg gccacggcgg cgatcttccg
     5161 ctgcttctac gcccttcccc tcctcgccgt cctggcgtgg aacgaacgcc gccggtacgg
     5221 gcccagggcg ccgggccagg cgcgcctggc ctggatcgcg ggcgtcttct tcgcgctcga
     5281 cctcgtgctg tggcaccact cgatcgagga ggtcggcgcg gggctcggga cggtgctcgg
     5341 caatacgcag gtcgtcgtcg tgccgctggc cgcgtggctg ctgctgggtg agcgacccgg
     5401 ggcgcgcgtg gcggcctccg tgccggtggt gctgatcggc gtcgtgctca tctcgggcgt
     5461 cctggacggc ggcggcgcgt atggccgcaa cccgctgctc ggcgtcctgt tcgggatcgg
     5521 gaccgacatc gcctacgccg gtttcctcct ggtccagcgc cgcgccaacg ccgaccaccg
     5581 acggccagcc ggtccactgt tcgacgccac gctctcggcg gccatcatgt cgctgctcat
     5641 cggcctgccg ctcggcgagg tcgacctggt gccgacgtgg ccggcgcacg gctggctgat
     5701 cctgctcggc gtcgccgtgc aggtggtcgg ctggttgctc atcagcatca gccttccgag
     5761 gctgccggca gcgatcacgt ccgtggtgct caccatccag ccggtggggt cggtcctgct
     5821 gggcgtggtg ctcctggcgg aggccccatc ggccttccag ctggtgggcg tgctcttcat
     5881 cctggccggg ctgctgctga cgacgctgcg ggtccgtccg catcgctggc gccgggcaca
     5941 gccggaggtg ggctaggcgg gatcgggcgc cgtgctggcg tggagcggga ggtgcacctc
     6001 gaagcaggtg cggccgggct ccgatgagac gtcgatccag ccaccgtgct gagcgaccac
     6061 ggtgtgcgag atgtggaggc ccaggcccgt gcccttgccg ggcggcttgg tcgtgtagaa
     6121 cggctcgaac aggcgcgcgc gcacctcggc cgggatgccc gggccggagt cgcagacgca
     6181 gacgaggacg ccgtcgccat ccggatccgg ctcggcgcgg atcgtcaacg agccacgacc
     6241 gcccatggcg tcgatggcgt tgtcgacgag gttcgtccac acctggttca gctcggagcc
     6301 gtgtgcctcg atctcgggca ggtcctcggc gagctccgtc cgcacgtcga tgccgtcccg
     6361 cagccgatga cgcaggatga cgagcgtctg ctggatgccg gttcgcacgt ccacgcgctg
     6421 cacgggggcc tggtccaggt aggcgtagcc cttgaccgcc gcgacgatct cgctgatccg
     6481 gcccagcgcc atcgacagct cggccagcag ctggtggacc gatgcgtccg ccgcgatcca
     6541 cgggcgcacc tcggccggct gcgccgccag ggactccggg gtccacccat cgtccaccag
     6601 cgcctgcgcc gcttcgtagc cgaccagctc ggccaccgcc tcgatgcgat ccccgcgctc
     6661 cagcgcgccg accgggggcc tcgcgtcggc cggtggccgc ggaggcggca gcgggcgggg
     6721 cagcgactcg gccgtggcga tggcctcgcc cagcgacctc acggcgcgca tggcggcggc
     6781 ggccgggttg ttgagctcgt gcgccaggcc cgcggcgatc gtccccaggc cggcgagctt
     6841 ctcgcgctcg cggagcccgg cctccatgcc ccgcagccgt tcgacggcgg tccggatgac
     6901 ggccagcgcc acgtccggcc ccgcggacag cagctcgcgc atggcctcga tcgggatgcg
     6961 caggacctcg gcctccccga cggccatgac cgaggccagc cggcgcccac cctccagcgc
     7021 cccgatctcg ccctgcagcg cgccggggcc gacccgggcc agcgggatct cgcttgtccc
     7081 ggcgcgcttg gtcacgtcga gctcaccgtc caggaccacg tagagggcat cggccacgtc
     7141 gccctcgttg atgagccgat cccccggcgc caggtcgacg atctcgccca tccgggtcag
     7201 ccgctccagc tggtcgtccg cgaggccgcc gaacagcggc gtggcccgca ggcgctccgc
     7261 gatgtcgctg atcggcaggt gctggtgcgg ctggtcgctc accggtggct cacaggtccg
     7321 ccaggtactg gtggacgaac tgcacggcca tcgcgccctc gccgacggcg ctggcgatgc
     7381 gcttgatgga ccgatgccgc acgtcgcccg ccgcgaacag gcccggcagg ctcgactcga
     7441 ggaggtacgg ctcccgatcc aggttccagc cctgggccgc gccgacgtcg gagccggtga
     7501 ggatgaagcc cttctcgtcg cgcgccacca cgccgtccag ccacgcggtg cgcggctgct
     7561 gcccgatgaa gacgaacgcg gcggcgagcg gcatctcgag ctcgccgtcc ggcgccgcga
     7621 aggtcgcgcg ctccaggtga tcggtgccgt ggaaggcgcg cgtatcggtc cgcgtgtgca
     7681 cctcgatggt cccgatggtg ccgatctgct cgacgaggta ggcgctcatg gtcgccgcca
     7741 ggtcgtcgcc ccgcacgatc aagtggacgc ggccggcgaa gcgcgacagg tgcacggccg
     7801 cctggcccgc cgagttgccg ctgccgatga cggccacgtc cgtgtcgcgg tagaggatcg
     7861 cctccgtcgt ggcagctccg tagtacacgc ccgcgccggc cagctccgcc gcgcccggca
     7921 cctcgagctg ccgatagctc acgccggtcg ccacgatcac cgcctggcac gacagctcgg
     7981 caccgtcctc gagcacgacc gtgcggtacg ggtcgccgcg acgaatcgag gcgacctcct
     8041 gcgacgagag cagctccgcg cccagccggc gcgcctgcgc cagcgcccgg cgcgccaggt
     8101 cgctgcccgt gaggccggcg gggaagccga ggtagttctc gatgcggctc gtcgtgcccg
     8161 cctggccgcc cggcgcctcg cgctcgacca gcagcgtctt gaggccctcg ctggcgccgt
     8221 acacggccgc ggccaggccg gccgggccgc tgccgacgat caccaggtcg tagaacggca
     8281 gcgccgcgtg cgtcgagagg ccgaccgcgt tcgccaggtc gcgcgtgctc gggtcgcgca
     8341 gcacggcacc accgggcagg aggaccaccg gctccccggg cgccagcggc gggtcgagcg
     8401 tcgccgccag gcgacgacca tcctcctcgg tggggtcgac ccaggcgtac ggcagctggt
     8461 tgcgcgtgag gaagtcgcgc agcgcgtgcc cgcgcggagc ccatcggtca gacagcaggc
     8521 gcaggccctc gaacggcggc cggaaggcgg cgttccagtc ggccagcagg tcgtccagga
     8581 ccgggtagag ccggtcgtcc ggcggctccc acggcttgag gatgtactgg tccagccgga
     8641 tctcgttgat cgcgtcgatc gcgacctcgg tgtcggcgta ggcggtcagc aggatccgct
     8701 tcgcgtccgg ctggagtggg agcgcctcgc gaagcagctc gatgccggtc atcaccggca
     8761 tgcgctgatc gacgacgaag agcgcgatca cgtcgccccg gcgggtcacc tcgcggctga
     8821 gctcgagggc ctccgggccg gagcccgcgg cgagcacgcg gtatcggtcg ccgtatcggg
     8881 cgcgcaggtc gcgctcgacg gatcggagga ccggttcgtc gtcgtcgacg gtgagaatcg
     8941 ccggcagggc ggccatgcgc ccgagtgtac gccgccgtgc gctaccctca ccccatgctg
     9001 tggaccatcg tcgtcatcct cctcctgctc tggttcctcg ggttcctgct gcgagccttc
     9061 ggcggcctca tccacctgct cctgatcgcg gcgctcgtca tcgtcatcta ccgcctgctc
     9121 accggacgca agctgctctg agccgatgct gctgaccggc accggtcgcc tggcgccgat
     9181 cggcctgtcg ccctgggcga tccgcatcga cgacgagcgc atcgcctgga tcggcgctcc
     9241 ccacgaggcc gatgacggcg agcgcatcga cctcggcaac gccctggtca ctccaggcct
     9301 ggtcgactcg cacacccacc cggtctttgc cggcgaccga tccgacgagg cggccgcccg
     9361 gctggagggg gcgcgataca ccgatggcgg catccttcga acgatgcgcg ccacgcgcgg
     9421 cgcc
//
LOCUS       DASTYP010000092         5769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1499818, whole genome shotgun sequence.
ACCESSION   DASTYP010000092 DASTYP010000000
VERSION     DASTYP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499818"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1638)
                     /locus_tag="VFV59_04430"
     CDS             complement(136..1638)
                     /locus_tag="VFV59_04430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5325110.1"
                     /translation="MVGKGGPSRAAPRGALAALVGVVVTAVLLGLQLPADPATGVTFS
                     MSPFTDEGWSVLPARNQVLLGRWIVDDWPLVWVQAPFSAVMAVAFSAFGVGIIQARLV
                     SIVVSIVAVGLIGGLLARRFGEAAGVVAGVGLATCALFLYYGRLALLEPMVTMLLVAG
                     AAVLLRRPRIPGAVGTDLGASLAAALLFGLAIATKASAAAAIVGLLVGAALAVTGGGE
                     ATRAMRRRLATAAIATAAIGIGYAAIVVAQPGLTAAIARIWPDATGPDSVVEAITRAL
                     SYVRGSDGAIGLTAPMIAGSLVGVALLVARWRLMRPEQRVLAGAAVGWLVAGLGLLLI
                     VDYRPNRYVVPMLPPMAVLTGIGAGIGLDLLGGRWSAWRRPAAVTAAATVVAILLGAA
                     GIGTLAGWMSTATYRLPEIQAEVLELADDGRPIQGAVTMAMRVPVPTLIVQSDVNAGD
                     LYDSPGVRWLLTNRDATPAWAAAHPEAWAARETLACYPWPSGEACLIRVP"
     gene            1783..2814
                     /locus_tag="VFV59_04435"
     CDS             1783..2814
                     /locus_tag="VFV59_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5325111.1"
                     /translation="MPDEPAIHARGVTRIYKAKPDPVTALAGVDLDVEPGEYFGLLGP
                     NGAGKTTLIKILTTLLLPTSGTAKVAGFDVVHETSKIRRVINMVAGGEQSGYGLLTLR
                     EQLWMFSQFYGLEQREGWRRVDELIEITGLTEQRDQKVRTLSTGQRQKLNFARGLLND
                     PWVLFLDEPTLGLDVAAARDLREHTLEWKAAAPGRTVLLTTHYMVEAEQLCDRIAIVD
                     RGRILALGTPDELRRRVQAESIFRIELDQLPHDGDLDAIAALPGVLSAVRADEDGDGA
                     ASDRVAIKVALADDSALTTVVTGVAQQGAHLIGLAKSEPSLEDVFVELVGRGFGDDDA
                     PGNAKGAAE"
     gene            2811..3689
                     /locus_tag="VFV59_04440"
     CDS             2811..3689
                     /locus_tag="VFV59_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325112.1"
                     /translation="MTAWRGVAARNPREAAEWSRGRVIRTNLRAVFGRAYPRIIGLTR
                     EPSWVFFEIVLPFLAVSAFVFVYRALQAPEEFVGFVVIGGAMTAFWLNVVWMMAAQFY
                     WEKDQGNLELYFTAPMHLMSVLAGMAIGGLVMTSSRALAVIVIGSVVYGVSYQVSDPL
                     LLVAVFVLTMIPLYGLGMLFASLFLLWGREAFHLAQLLQEPIYFLGGVNFPIGPAFGQ
                     LAAIVMATLPLAVGLDAMRQLVFAGSETNGVLPVGAEMVILGVMGVVFIVGARIALGY
                     LETLARREGRLTLRWQ"
     gene            3680..4558
                     /locus_tag="VFV59_04445"
     CDS             3680..4558
                     /locus_tag="VFV59_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325113.1"
                     /translation="MAVIERTPRPAVPGFTFADAWRTFRTAIGLGWAVESNWSDPFLF
                     AVYTLAKPLAAAMILVVMFQVITGGQGTEFLQFMIVGSALWNVVFGVMGGLVQSILED
                     RERYRMMKYVVVTPSSLFPFLLGRSLARVLISFIAVAVTMLVGVAFLGVELRPNLLVL
                     VPALVLGIVAVIALGIFMAGWCLQLRQEAWSYPEAIAGALYLVSGAIFPVNVLPEFLR
                     PVAWISPTTWWLEASRRGLLGHGSPGDIGQLTDATVLLYLALSTAVVVPLALALFAWF
                     VRRARQAGLLDMTTGS"
     gene            complement(4654..5181)
                     /locus_tag="VFV59_04450"
     CDS             complement(4654..5181)
                     /locus_tag="VFV59_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325114.1"
                     /translation="MTREVRIGGAVVAALGAFIFFLMVVGSGGGTRPEIDPLSVQDVL
                     SGGPPANRYGSEELHISGWYAELAADCEGDDGGADASVAWLQRDCPLRVLLPEQPDED
                     VTQAELVAQGIRLAAPLGNAFPSRAEPTGPNRRGQELVFVGHFNDTAAAQCVPERQAR
                     CENTFVVTDYDELLR"
     gene            complement(5178..>5769)
                     /locus_tag="VFV59_04455"
     CDS             complement(5178..>5769)
                     /locus_tag="VFV59_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU5325115.1"
                     /translation="PLGTALRALQDSMPTCDEGARPVLSGDGTERAMLIGQAPGWREI
                     ETGLPFAWDAGKRLCGWLATAGVSIEDFRERWYVTSVGKCYPGRNDGASVDRPPSRRE
                     IERWTPFLLREVELVRPRLVLLVGGLAHRFAFGAGARLDELVGRELTWDRAPAASVLC
                     LPHPSGASTWLNDPGRVELWRSAIGLLRDRWAELDR"
BASE COUNT          813 a         2125 c         1999 g          832 t
ORIGIN      
        1 ccggtcgcgc ggcatctcca cgtcgaggtt cttgaggttg tgctcgcggg caccgcgaac
       61 gacgatcttg tccgagggca ttcgacgaac catcgtagca ggatctgcgc cgatgcgaac
      121 gcctgttcgg tttgcctacg gcacgcggat gaggcacgcc tcgcccgatg gccacgggta
      181 gcaggcgagc gtctcgcgag ccgcccaggc ctcgggatgc gcagccgccc aggcgggcgt
      241 cgcgtcacgg ttcgtcagca gccagcgaac accgggcgag tcgtacaggt ccccggcgtt
      301 gacgtcggat tggacgatca gcgtcggcac cggcacgcgc atcgccatgg tcacggcacc
      361 ctggatcggg cggccgtcat cggccagctc cagcacctcg gcctggatct ccggcagccg
      421 atacgtcgcg gtgctcatcc agccggcgag cgtgccgatg cccgcggcgc cgagcaggat
      481 cgcgacgacc gtggcggccg ccgtcaccgc ggcgggtcga cgccatgcgg accagcggcc
      541 gcccagcagg tccagcccga tcccggcgcc gatgccggtc agcacggcca tgggcgggag
      601 catcggcacc acgtagcgat tcggacggta gtcgacgatg agcagcaggc ccaacccggc
      661 caccagccat ccgacggcgg cgccggccag cacgcgctgc tcgggccgca tgagccgcca
      721 gcgggcgacc agcagcgcca cgccgaccag gcttccggcg atcatcggcg cggtgagccc
      781 gatggcgccg tcggagccac ggacgtagct gagtgcccgc gtgatcgctt cgacgaccga
      841 atcgggcccg gtggcgtcgg gccagatcct ggcgatggcg gcggtgagcc cgggctgcgc
      901 gaccacgatc gccgcatacc cgatgccgat cgccgccgtc gcgatggccg cggtcgccag
      961 ccggcggcgc atggcccgcg tcgcctcgcc tccgccggtc accgccagcg ccgcacccac
     1021 cagcagcccg acgatggcgg cggccgcgga cgccttcgtc gcgatcgcca gcccgaagag
     1081 cagcgccgct gcgagtgagg cacccaggtc ggtgccgacg gcaccgggga tcctcggccg
     1141 ccgcagaagg acggcggccc ccgccacgag cagcatggtc accatcggtt ccagcagggc
     1201 aagccggccg tagtacagga acagggcgca ggtcgccagg ccaacccccg cgaccacgcc
     1261 ggccgcctcg ccgaaccgcc gtgcaaggag cccgccgatc aggcccacgg caacgatcga
     1321 cacgacgatc gacacgagac gcgcctggat gatcccgacg ccgaacgcgg agaaggcgac
     1381 agccatcacc gcgctgaacg gggcctgcac ccagaccagc ggccagtcgt cgacgatcca
     1441 ccggcccagg agcacctggt tgcgtgcggg gagcaccgac cagccctcgt ccgtgaacgg
     1501 cgacatgctg aacgtcacgc cggtggccgg gtcggcgggc agctggagac cgaggagcac
     1561 cgccgtcacg acgacgccca ccagtgccgc caacgcgccg cggggagcgg cgcggctcgg
     1621 gccccccttc cccaccacgt cgcgatccta gcgcgccgtg ccctcccggc gcgcgcacga
     1681 cgcacggaat acgcgacgga tgacggatgc gtggccgggt gttgcggtgc tacccttcgc
     1741 gacccgcccg ccgacccgac ctcggagacc gtctgcccgc ccatgcccga tgaaccggcg
     1801 atccatgccc gcggggtgac ccgcatctac aaggcgaagc ccgatccggt gaccgccctg
     1861 gccggcgtcg acctcgacgt cgagcccggt gagtacttcg gcctgctcgg cccgaacggc
     1921 gccgggaaga ccacgctgat caagatcctc acgacgctgc tgctccccac gagcgggacc
     1981 gcgaaggttg ccggcttcga cgtggtccac gagacctcga agatccggcg cgtcatcaac
     2041 atggtcgccg ggggcgagca gtcgggctac ggactgctga cgctgcgcga gcagctgtgg
     2101 atgttcagcc agttctacgg cctcgagcag cgcgagggct ggcgccgcgt ggacgagctc
     2161 atcgagatca ccggcctgac cgagcagcgg gaccagaagg tgcggacgct ctcgaccggc
     2221 caacggcaga agctgaactt cgctcgcggc ctgctcaacg acccgtgggt cctcttcctt
     2281 gacgagccga cgctgggcct ggacgtggcc gccgcccgcg accttcgcga gcacaccctt
     2341 gagtggaagg ccgcggcgcc gggtcgcacg gtgctcctca cgacgcacta catggtggag
     2401 gcggaacagc tgtgcgaccg catcgccatc gtcgaccgcg gccgcatcct ggcgctcggg
     2461 acgcctgacg agctgcggag gcgggtgcag gccgagtcga tcttccgcat cgagctcgac
     2521 cagctgccgc acgacggcga cctcgacgcc atcgccgccc ttcccggcgt gctctccgcg
     2581 gtccgtgccg acgaggacgg cgacggcgcc gcatccgacc gcgtcgcaat caaggtcgcg
     2641 ctggcggacg acagcgcgct gacgaccgtg gtgaccggtg tggcccagca gggcgcccac
     2701 ctcatcggcc tggcgaagtc ggagccgagc ctggaggacg tgttcgtcga gctcgtcgga
     2761 cgcggcttcg gcgacgacga cgcgcccggg aacgccaagg gggccgcgga gtgacggcct
     2821 ggcgcggcgt cgcggcgcgg aacccgcggg aggcggcgga gtggtcgcgg ggccgcgtca
     2881 tccgcaccaa cctgcgcgcc gtcttcgggc gtgcgtaccc ccggatcatc ggcctcaccc
     2941 gcgagccgtc ctgggtgttc ttcgagatcg tcctgccgtt cctggccgtc agcgccttcg
     3001 tcttcgtcta ccgcgcgctt caggcgccgg aggagttcgt cggcttcgtc gtcatcgggg
     3061 gcgccatgac ggcgttctgg ctgaacgtcg tgtggatgat ggccgcgcag ttctattggg
     3121 agaaggacca gggcaacctg gagctctact tcacggcgcc aatgcacctg atgagcgtcc
     3181 tggccggcat ggccatcggc gggctggtga tgaccagctc gcgggcgctg gccgtgatcg
     3241 tcatcggctc cgtcgtctac ggcgtgagct accaggtcag cgatccgctc ctgctggtgg
     3301 cggtcttcgt gctgacgatg atcccgctgt acggcctcgg gatgctgttc gccagcctct
     3361 tcctgctgtg gggacgcgag gcgttccacc tggcccagct cctccaggag ccgatctact
     3421 tccttggcgg cgtcaacttc cccatcggcc ccgccttcgg tcagctggcg gccatcgtca
     3481 tggcgacgtt gccactggcc gtgggcctcg acgccatgcg ccagctcgtc ttcgccggca
     3541 gcgagacgaa cggcgtgctc ccggtcggcg ccgagatggt gatcctcggc gtcatgggcg
     3601 tcgtcttcat cgtcggggct cgaatcgcgc tcgggtacct cgagaccctc gcgcggcgcg
     3661 aggggaggct gacgctgcgg tggcagtgat cgagcgcacc ccgcgcccgg ccgtgccggg
     3721 cttcaccttc gcggatgcct ggcggacctt ccgcaccgcc atcgggctcg ggtgggcggt
     3781 agagtcgaac tggtcggatc ccttcctctt cgccgtctac acgctggcga agccgctcgc
     3841 cgccgccatg atcctcgtgg tcatgttcca ggtcatcacc ggcgggcagg gaaccgagtt
     3901 cctccagttc atgatcgtcg gcagtgcgct gtggaacgtc gtgttcggcg tcatgggtgg
     3961 cctggtccag tcgatcctcg aggaccgcga gcgctaccgg atgatgaagt acgtcgtcgt
     4021 cacgccctcg tcgctgttcc cgttcctgct gggccggtcc ctggcgcgcg tgctgatctc
     4081 gttcatcgcc gtggccgtga ccatgctcgt gggcgtcgcc ttcctcggcg tcgagctgcg
     4141 accgaacctg ctggtcctgg tgcccgccct ggtcctcggg atcgtcgccg tcatcgcgct
     4201 cggcatcttc atggccgggt ggtgcctgca gctgcgccag gaggcgtggt cctaccccga
     4261 ggcgatcgcc ggggcgctgt acctggtctc cggcgcgatc ttcccggtga acgtcctgcc
     4321 cgagttcctg cggccggtcg cgtggatctc ccccaccacc tggtggctcg aggcgtcgcg
     4381 tcgtgggctg ctgggccatg gctcaccggg ggacatcggg cagctcaccg acgcgaccgt
     4441 cctcctctac ctcgccctca gcacggcggt ggtcgtgccg ctggcactgg cgctcttcgc
     4501 gtggttcgtc cgccgggcgc gccaggccgg actgctggac atgaccaccg gctcctgatc
     4561 gccgatacga gcgtatctgt cgccgggtga tgcctatccg cggcgtcgct gggctacaga
     4621 gatcgagggc tgcctccgct ccacgcggcg ccgctagcga agcagctcgt cgtagtccgt
     4681 cacgacgaac gtgttctcgc accgcgcctg ccgttccggc acgcactgcg cggcggcggt
     4741 gtcgttgaag tgaccgacga acaccagctc ctggccgcgg cggttgggtc ccgtcggctc
     4801 ggcacgcgac gggaaggcgt tgccgagcgg cgccgcgagc cggatgccct gcgccaccag
     4861 ctccgcctgc gtcacgtcct cgtcgggctg ctccggcagc agcacgcgca gcgggcagtc
     4921 gcgctgcagc cacgcgacgg acgcatcggc gcctccgtcg tctccctcgc aatcggcggc
     4981 cagctcggcg taccagccgc tgatgtgcag ctcctcgctg ccgtagcggt tggccggcgg
     5041 gccgccggac agcacgtcct ggacgctcag ggggtcgatc tcgggacgcg tcccgccacc
     5101 ggacccgacg accatcagga agaagatgaa ggcacccagg gcggccacga ccgcgccgcc
     5161 gatacggacc tcccgggtca tcggtccagc tcggcccagc ggtcccgcag caggccgatc
     5221 gccgagcgcc acagctccac ccgtcccggg tcgttgagcc acgtcgacgc gcccgagggg
     5281 tggggcaggc acaggaccga ggcggccggc gcccggtccc acgtgagctc acgccccacg
     5341 agctcatcca gccgagcgcc ggcgccgaac gcgaaccggt gcgccagccc gccgaccagc
     5401 agcacgaggc gcggccggac gagctccacc tcccggagca ggaacggcgt ccagcgctcg
     5461 atctcgcgtc gcgacggcgg gcggtcgacc gacgccccgt cgttccggcc ggggtagcac
     5521 ttgccgacgc tggtcacgta ccagcgctcg cggaagtcct cgatgctcac ccccgcggtc
     5581 gccagccagc cgcagagccg cttcccggcg tcccaggcga acgggagtcc cgtctcgatc
     5641 tctcgccagc ccggggcctg gccgatgagc atcgcccgct ccgtgccgtc gccggacagg
     5701 acgggacgcg cgccctcgtc acacgtcggc atcgagtcct gcagcgcgcg cagcgccgtc
     5761 ccgagcgga
//
LOCUS       DASTYP010000093         7597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_916199, whole genome shotgun sequence.
ACCESSION   DASTYP010000093 DASTYP010000000
VERSION     DASTYP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916199"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFV59_04460"
     CDS             complement(<1..684)
                     /locus_tag="VFV59_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU5325116.1"
                     /translation="MLVPLSWLREYVDIEMSPRQLADALTMRGMEVAAIETVGGDWSN
                     VVVGRVLDVERHPNADTLWLTRVDVGRAGGELQIVCGAQNLEVGQLVPAALPGAVLPG
                     DRRIERSKIRGVVSNGMLCSAAELELGGDADGIHILGHDADLAPGTPLSEAIGETVLD
                     VDVKPNRGDALSMVGLAREIAALTGAELRLPSVEVAEHDEPTEQHVSVRIEEPELNPR
                     FTARYFEGVR"
     gene            complement(685..1767)
                     /gene="pheS"
                     /locus_tag="VFV59_04465"
     CDS             complement(685..1767)
                     /gene="pheS"
                     /locus_tag="VFV59_04465"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU5325117.1"
                     /translation="MTDLRTDLESLRVTARERLASAATADAVEALRHELLGRSGSLTG
                     LLRQLGSVPAADRPTLGQLANEVRGELESAILERLAQLRDADLDARLADEALDMSAPG
                     RPMPVGHLHPVHTTIRDVRELFHAYGFEVFEGPEIETEEYNFELLNIPPDHPARDLWD
                     TLYVAEPGSVEAGEPRPAADETILRTHTSPVQIRAMRALEPPIRVLMPGRCFRYEAVD
                     ASHGFEFFQVEGLVVDRDTSMADLKGLLEEVARALYGRDVRTRFRPGYYPFTEPSVAF
                     DIGCLVCGGAGCPACKRSGWMTILGAGMVHPVVLRAGGYDPDEYQGYAFGMGTDRMTM
                     LRHGIGDIRLFFTGDLRFVTQFAEGR"
     gene            complement(1861..2220)
                     /gene="rplT"
                     /locus_tag="VFV59_04470"
     CDS             complement(1861..2220)
                     /gene="rplT"
                     /locus_tag="VFV59_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU5325118.1"
                     /translation="MARVKRGVAAHRRHKRLLNLAEGRRGTRSTLIRPAREALIHALV
                     YAYRDRRDRKRDMRRLWIERINAASRQHGLPYGRFIAGLRAAGVEVDRKVLADMAVRE
                     PAAFAAMVDTAKASFTS"
     gene            complement(2224..2433)
                     /gene="rpmI"
                     /locus_tag="VFV59_04475"
     CDS             complement(2224..2433)
                     /gene="rpmI"
                     /locus_tag="VFV59_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU5325119.1"
                     /translation="MPKMKTHKGTAKRYKKTGSGKWIRPKAWRGHHLEIKSSRRTRRY
                     DGKAIVSDAQVGQLERLMPYAGKGK"
     gene            complement(2436..3083)
                     /gene="infC"
                     /locus_tag="VFV59_04480"
     CDS             complement(2436..3083)
                     /gene="infC"
                     /locus_tag="VFV59_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU5325120.1"
                     /translation="MARDLRINEMIRIREVRVIDDQGEQLGVMPTFRALALAQEKGLD
                     LVEVAPTAVPPVCRILDYGQYKYELQKREREQKKKQKSQTFKEVRLGVKVDVNDLKTK
                     ARRAAEFLDHGDRVKVTVRFRGREISHANLGRDLLIRTAALLEEHGTIERQPLLEGRS
                     MYLVMAPLERRAERKTDSDGSSEPAGETIGDAVAARAPAAPAPQATPEAPAQQGP"
     gene            3451..4944
                     /locus_tag="VFV59_04485"
     CDS             3451..4944
                     /locus_tag="VFV59_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEU5325121.1"
                     /translation="MTTSTLTPLRVSTALAVAALLGGMLAAGSVRAHDPVLGVDPASV
                     ELTLAPGTSDDVAKTVHTPEVLPTPDIYFLADSTGSMGDVIANVQGDAASVLASVDAM
                     ASDPRYGAGDYKDFQSSQADPYAFNNGAAIPAADDNGAAALAAIGAWSASGGFDGPEG
                     QLYALHRLAAHGDAGFRSDSTPIVVWFGDAPAHDPVCDEISGDTGHDLTEASVTAELV
                     AAGVRVIAISVVTASGSFYPAALDDDPTAFGGNYAADCGTEGGTSGQASRIAAATGGV
                     HLTGVAATDIADAILAGLGALPVDVTPQYSCDDASVTVTWDAASKTVTSGEDATFTET
                     VAAGAGAPQGETVTCTVDFLVDGELLAGFTQTITVDIPDVTAPTVACAEGTNPHGRNV
                     PRATNQNPDGFYLLSADDNVDTDPSIWVVDAGSGEVFGPYSSGTTVKYTQAPGATPGE
                     KPIGSTNGAAGAVTVHIIGTGDMQVYATDASDNVSTAASCLVPPPPR"
     gene            complement(5014..5370)
                     /locus_tag="VFV59_04490"
     CDS             complement(5014..5370)
                     /locus_tag="VFV59_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5325122.1"
                     /translation="MRGVGQLALRSTEPNRVAEFYESAFGFRRLAGDDAGVDLELGGF
                     ELSFRRGETATRDGIQIGFKVDTASEVDAWAQELGRRGFALAAPPSGDRWEGSRSFRI
                     ADPDGRQVAVFYDYPR"
     gene            complement(5393..7204)
                     /gene="thrS"
                     /locus_tag="VFV59_04495"
     CDS             complement(5393..7204)
                     /gene="thrS"
                     /locus_tag="VFV59_04495"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5325123.1"
                     /translation="MAIDADEIGQDASAALASGREDHLAAMRHSAAHMMAAAVLEVFP
                     DARLGIGPAIKDGFYYDFDLPRPLTPDDLEQIEEKMRAQQAADLAFEHSRDLPREEAI
                     GLNRERNQPFKVEIIQDLPESEGPTVSFYRHGTFEDLCRGGHLESTAQLGAFKLLHTA
                     GAYWRGDETRPMLQRIYGTAWDTQEELDRYLWRIEEARKRDHRKLGRELDLYTFHPES
                     PAAPFLHPRGMAIWRALEDWSREVRRGAGFHEVRTPSLVRKELWETSGHWANYQDNMF
                     VLDDYDHVSGLKPMNCPEEILIYKTRARSYRDLPIRFADYSVLFRKERTGVLAGMFRV
                     RQLTQDDSHVFCREDQLDDELRLALSLVRKQYAPFGFEPKARLATRPEKRIGSDEFWE
                     RAENRLRDRLEEDGMSYALDEGGGAFYGPKIDIFIEDALGREWQMATIQADYQLPQRF
                     DLEYRAADGGFERPVIVHYAIYGSFERFIGIITEEYAGAFPLWLAPVQVMVVPIADRH
                     LDYAERVRVALADAGLRAEVDSRSERMQAKLREAQEQKIPVMVVVGDRDQEATAVSPR
                     LRTGESHQGVALDEFVADLVSRNAARELWPFADEAAS"
     gene            complement(7256..7492)
                     /locus_tag="VFV59_04500"
     CDS             complement(7256..7492)
                     /locus_tag="VFV59_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325124.1"
                     /translation="MINRDNPQGPGQGPLDRPSRDAGDPIGDNAYPGGSGMLPPTADM
                     DDPIPETGMPGEPRAEPDQLEEEEELGPEDEPRA"
BASE COUNT         1068 a         2735 c         2647 g         1147 t
ORIGIN      
        1 gcgcacgccc tcgaagtacc gcgcggtgaa gcgcgggttg agctccggct cctcgatgcg
       61 caccgagacg tgctgctccg tcggctcgtc atgctccgcg acctcgaccg acggcaggcg
      121 cagctccgcg ccggtcaggg ctgcgatctc acgagccagg ccgaccatcg acagcgcatc
      181 gccgcggttc ggcttgacgt cgacgtcgag cacggtctcc ccgatcgcct cggacagggg
      241 cgtgcccggc gccaggtccg cgtcgtgacc gaggatgtgg atgccgtccg cgtcgccacc
      301 cagctcgagc tccgccgcgc tgcacagcat cccgttggag acgacgccgc ggatcttcga
      361 ccgctcgatg cgtcgatcgc ccggcagcac cgcccccggc aacgcggccg gcacgagctg
      421 cccgacctcg aggttctgcg cgccgcacac gatctgcagc tcaccgccgg cacgccccac
      481 gtcgacgcgc gtcagccaca gggtgtcggc gttcgggtgc cgctcgacgt cgaggacgcg
      541 cccgaccacg acgttcgacc agtcacctcc gaccgtctcg atggcggcga cctccatgcc
      601 gcgcatggtc agcgcatcgg ccagctggcg cggcgacatc tcgatgtcga catactcgcg
      661 cagccacgag agcgggacca gcacctagcg gccctccgcg aactgcgtca cgaagcggag
      721 atcaccggtg aagaagagcc ggatgtcccc gatgccgtgg cgcagcatcg tcatgcgatc
      781 ggttcccatg ccgaaggcgt agccctggta ctcgtccggg tcgtagccgc ccgcccgcag
      841 cacgaccggg tgcaccatcc cggcgccgag gatcgtcatc cagcccgagc gcttgcaggc
      901 cgggcagccc gcgccgccgc acaccaggca gccgatgtcg aacgcgacgc tcggctcggt
      961 gaacgggtag tagccaggcc ggaaccgcgt gcggacgtcg cgtccgtaca gcgcccgcgc
     1021 gacctcctcc agcaggccct tgaggtcggc catgctcgtg tcgcggtcga cgacgagacc
     1081 ctccacctgg aagaactcga agccgtggct ggcgtcgacg gcctcgtagc ggaagcagcg
     1141 tccgggcatg aggacgcgga tgggcggctc gagggcacgc atggcccgga tctggaccgg
     1201 cgacgtgtgc gtgcggagga tcgtctcgtc ggcggccggc cggggctcac cggcctcgac
     1261 gctccccggt tccgcgacgt acagcgtgtc ccacaggtcg cgcgccggat ggtcgggcgg
     1321 gatgttgagc agctcgaagt tgtactcctc ggtctcgatc tccgggccct cgaagacctc
     1381 gaagccgtag gcgtggaaca gctcccggac gtcccggatc gtggtgtgga ccgggtgcag
     1441 gtgcccgacc ggcatcgggc gccccggcgc ggacatgtcc agcgcctcgt ccgccaggcg
     1501 cgcgtcgagg tccgcgtcgc gcagctgcgc caggcgctcg aggatggccg actcgagctc
     1561 gccgcgcacc tcgttggcca gctgccccag cgtggggcgg tccgccgccg gcaccgatcc
     1621 cagctggcgc aggagcccgg tcaggctgcc cgatcgcccg agcagctcgt ggcgcagcgc
     1681 ctcgaccgcg tcggcggtcg ccgccgacgc caggcgctcg cgggcggtga cgcgcagcga
     1741 ttcgaggtcg gttcgcaggt cagtcatgga tcgtcgccca atcatcgggg aaaacgaacg
     1801 accccgtccc ggttctcggg acgaggtcgt gggtgaccag cgctcggcgc ggaacgctgg
     1861 tcagctggta aaggacgcct tggcggtgtc gaccatggcc gcgaaggcgg ccggctcacg
     1921 caccgccatg tcggccagga ccttccggtc gacctcgacg cccgcggcac gcagcccggc
     1981 gatgaatcgg ccgtacggca ggccatgctg gcgcgaggcg gcgttgatgc gctcgatcca
     2041 caggcggcgc atgtcgcgct tgcgatcgcg gcggtcgcga taggcgtaga cgagggcgtg
     2101 gatgagcgcc tcgcgcgccg ggcggatcag ggtcgagcgg gtgccgcgac ggccctcggc
     2161 gaggttcagc aggcgcttgt gacgccgatg cgcggcgacg ccgcgcttga ctcgggccat
     2221 cggctacttc cccttgccgg cgtacggcat gaggcgctcg agctggccga cctgcgcgtc
     2281 ggacacgatg gccttcccgt cgtatcggcg cgtgcggcgg ctcgacttga tctccaggtg
     2341 gtggccacgc cacgccttcg gccggatcca cttgccggag ccggtcttct tgtagcgctt
     2401 ggccgtgccc ttgtgggtct tcatcttcgg cacgtctacg gtccctgctg tgcgggcgcc
     2461 tcgggcgtcg cctgcggcgc cggcgctgcc ggggcccggg cggcgaccgc gtcgccgatg
     2521 gtctcacccg ccggctcgga cgagccgtcg gaatcggtct ttcgttccgc gcgtcgctcc
     2581 agcggcgcca tgacgaggta catgcttcgt ccctcgagca gcggctgccg ctcgatggtg
     2641 ccgtgctcct cgaggagggc ggcggtgcgg atcagcaggt cgcgtccgag gttggcgtgg
     2701 ctgatctccc gcccgcgaaa gcggacggtc accttcacac gatcgccgtg atccaggaac
     2761 tcggcggcac ggcgggcctt cgtcttcagg tcgttgacgt cgaccttgac gccgagccgg
     2821 acctccttga aggtctgcga cttctgcttc ttcttctgtt cgcgctcgcg cttctgcagc
     2881 tcgtacttgt actgcccgta gtcgaggatg cggcacacgg gagggacggc agtgggagcc
     2941 acctccacga ggtcgagacc cttctcctgc gccagggcga gggctctgaa ggtcggcatc
     3001 acgccgagct gctctccctg atcgtcgatg acccgtacct cgcggatccg gatcatctcg
     3061 ttgatgcgaa ggtcccttgc cagtgcctgc tctccttcaa caacaaggcc acctggccga
     3121 tcacggacgg gtggccctgc aaacgtacgc gaattgcccg cggatgatac gcgagatcgg
     3181 ggtcacggtc aacgcgaccg tgcggtgtgt cgcggcggcg cggatacccg gggtcgacac
     3241 gttggccacc tcgcgccaaa ggtccggctg gccatcgcca tttgccttcg taggatcggt
     3301 gcggaggctc acgccgccga tccgagacgg cgcgcgccgg tctgagaggg cgacggtgtg
     3361 gcgcacccga ccgcggtcac gagaggacca cttccgcggc ggccgccggc acccgaccct
     3421 gacgagccat ccagcctcgg agggtactcc atgaccacat ccaccctcac cccgcttcgg
     3481 gtatccacgg cgctcgccgt ggccgccctc ctcggcggca tgctggccgc gggttcggtc
     3541 cgcgcccacg atcccgtcct cggcgtcgac cccgccagcg tcgagctcac gctcgcgccg
     3601 ggcaccagcg acgacgtggc caagacggtg cacacgcccg aagtcctccc cacgccggac
     3661 atctacttcc tggccgactc caccggcagc atgggcgacg tcatcgcgaa cgtgcagggc
     3721 gacgccgcca gcgtgctggc ctcggtcgac gccatggcga gcgacccgcg gtacggcgcc
     3781 ggcgactaca aggacttcca gagctcgcag gccgatccgt acgcgttcaa caacggcgcc
     3841 gccatcccgg ccgccgacga caacggcgcg gcggcgctgg cggccatcgg cgcctggtcg
     3901 gccagtggtg gcttcgacgg ccccgagggc cagctgtacg ccctgcaccg tctggccgcc
     3961 cacggggacg cgggcttccg gtccgattcc accccaatcg tggtgtggtt cggcgacgcg
     4021 ccggcgcacg atccggtgtg cgacgagatc tcgggcgaca ccggccacga cctgaccgag
     4081 gcgtcggtca ccgccgagct ggtggctgcc ggcgtccggg ttatcgccat cagcgtggtg
     4141 accgcttccg gttccttcta cccggccgcg ctcgacgacg acccgacggc gttcggcggc
     4201 aactacgcgg ccgactgcgg gaccgagggc ggcaccagcg ggcaggcgtc gcggatcgcg
     4261 gcggccaccg gcggcgtgca cctgaccggc gtcgcggcga ccgacatcgc ggatgccatc
     4321 ctggcgggcc tcggcgcgct ccccgtcgac gtcacgccgc agtactcctg cgacgatgcc
     4381 agcgtcacgg tgacgtggga tgcggcgagc aagaccgtga cgagcggcga ggacgccacc
     4441 ttcaccgaga ccgtcgcggc cggcgccggc gcgccacagg gcgagaccgt gacgtgcacg
     4501 gtcgacttcc tggtggacgg agagctgctc gccggcttca cccagacgat caccgtcgac
     4561 atccccgacg tcaccgcgcc gacggtcgcg tgcgccgagg gcaccaaccc gcatggcagg
     4621 aacgtgccgc gggcgacgaa ccagaacccg gacggcttct acctgctgtc ggccgacgac
     4681 aacgtggaca ctgatccgtc aatctgggtc gtcgacgccg gcagcggcga ggtcttcggc
     4741 ccgtactcgt cgggcacgac ggtgaagtac acccaggcac ccggtgcgac gccgggcgag
     4801 aagccgatcg gcagcaccaa cggtgcggcc ggcgcggtga cggtgcacat catcggcacc
     4861 ggagacatgc aggtctacgc cacggacgcc tccgacaacg tgtcgacggc cgcctcctgc
     4921 ctggtgccgc ctccgccacg ctgatcacgg taggcgcccc atcgtaaacc gatacgaggg
     4981 accggtctcc ggaccggtcc ctcgccctcc ccgtcagcgc gggtagtcgt agaagaccgc
     5041 cacctgccgg ccatcgggat ccgcgatgcg aaagctgcgg ctgccctccc atcggtcccc
     5101 tgacggcggt gccgccagcg cgaagccccg gcggccgagc tcctgggccc aggcgtccac
     5161 ttcgctggcg gtgtcgacct tgaagccgat ctggatgccg tcgcgcgtgg cggtctcccc
     5221 gcggcggaag ctcagctcga agccaccgag ctccaggtcg acgccggcgt cgtcccccgc
     5281 caggcgccgg aagccgaagg ctgactcgta gaactccgcg acgcggttcg gttcggtcga
     5341 gcgcagcgcg agctggccga cccctcgcat cttcctcacg ggcgggccgc gctcaggacg
     5401 cggcctcgtc cgcgaacggc cagagctcgc gcgccgcgtt gcggctcacg aggtcggcca
     5461 cgaactcgtc gagcgccacg ccctggtgcg actcgccggt gcggagccgt ggcgagacgg
     5521 cagtcgcctc ctggtcgcgg tcgcccacca cgaccatgac agggattttc tgctcctgtg
     5581 cctcgcgcag cttggcctgc attcgttcgg atcggctgtc gacctcggcg cgaaggcccg
     5641 catcggccag cgcgacgcga acgcgctcgg cgtagtccag gtggcgatcg gcgatcggca
     5701 cgaccatcac ctggaccggt gccagccaca gcgggaaggc gccggcgtac tcctcggtga
     5761 tgatgccgat gaaccgctcg aagctgccgt aaatggcgta gtgcacgatc accggccgct
     5821 cgaacccgcc gtccgcggcg cggtactcga ggtcgaagcg ctgcggcagc tggtagtcgg
     5881 cctggatggt cgccatctgc cactcacggc cgagggcgtc ctcgatgaag atgtcgatct
     5941 tcgggccgta gaacgcgccc ccgccctcgt cgagcgcgta cgacatgccg tcctcctcga
     6001 gccggtcgcg cagccggttc tcggcgcgct cccagaactc gtcggagccg atgcgcttct
     6061 cgggacgcgt cgccagcctg gcctttggct cgaacccgaa gggcgcgtac tgcttccgca
     6121 ccagcgacag ggccaggcgc agctcatcgt cgagctggtc ctcgcggcag aagacgtgcg
     6181 agtcgtcctg ggtcagctgc cggacgcgga acatcccggc cagcacgccc gtgcgctcct
     6241 tgcggaacag caccgagtaa tccgcgaagc ggatcggcag gtcgcggtag gagcgtgccc
     6301 gggtcttgta gatcaggatc tcctccgggc agttcatcgg cttcagcccg gagacgtggt
     6361 cgtagtcgtc caggacgaac atgttgtcct ggtagttggc ccagtggccg gacgtctccc
     6421 acagctcctt gcgaaccagg ctgggcgtgc ggacctcgtg gaatcccgcg ccgcggcgca
     6481 cctcgcgcga ccagtcctcg agcgctcgcc agatggccat cccgcgcgga tggaggaacg
     6541 gcgcggcggg cgactcgggg tggaaggtgt acaggtccag ctcgcggccg agcttgcggt
     6601 ggtcgcgctt gcgagcctcc tcgatgcgcc acaggtatcg gtccagctcc tcctgcgtgt
     6661 cccaggcggt gccgtagatg cgctggagca tcggccgggt ctcgtcgccg cgccagtacg
     6721 cgccggcggt gtgcagcagc ttgaacgcgc cgagctgcgc ggtcgactcg aggtggccgc
     6781 cgcggcagag gtcctcgaac gtcccgtggc ggtagaagct gaccgtcggg ccctccgact
     6841 cgggcaggtc ctggatgatc tccaccttga acggctggtt ccgttcccgg ttcagcccga
     6901 tggcctcctc gcgcggcagg tcacgcgagt gctcgaaggc gagatcggcg gcctgctggg
     6961 cgcgcatctt ctcctcgatc tgctcgaggt cgtcgggcgt cagcgggcgc ggcaggtcga
     7021 agtcgtagta gaagccgtcc ttgatggcgg gaccgatgcc cagcctggca tccgggaaca
     7081 cctcgagcac ggcggccgcc atcatgtggg ccgcgctgtg gcgcatggcg gccaggtggt
     7141 cctcgcggcc cgatgcgagc gccgcgctcg cgtcctggcc gatctcgtca gcgtcgattg
     7201 ccatgcggcg aagggtagcg catgcggggg gccaggcggc ccgcccgccg aagcctcagg
     7261 ccctcggctc gtcctcgggc cccagctcct cttcctcctc gagctggtcg ggctcggcgc
     7321 gcggctcgcc aggcatgccc gtctcgggga tcggatcgtc catgtcggcg gtcggcggca
     7381 gcatgcccga ccctccgggg taggcgttgt cgccgatggg gtcgccggcg tcgcggctcg
     7441 gccggtcgag cggaccctgc ccgggtccct gcgggttgtc gcgattgatc atgcccgcga
     7501 ccgatgcaat cgctgcgcca gccgcgaccg cggcgttcag acgcgcttga actgctcgaa
     7561 cggctgccgg cagtcggcac agtggtagat cgctcgg
//
LOCUS       DASTYP010000094         5956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1245429, whole genome shotgun sequence.
ACCESSION   DASTYP010000094 DASTYP010000000
VERSION     DASTYP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245429"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VFV59_04505"
     CDS             complement(<1..849)
                     /locus_tag="VFV59_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU5325125.1"
                     /translation="MRPRRGRLADGRTILYFDDAGVTRGEPPADRRRLEPGPPPAELR
                     HDALLDEWVILAAHRQGRTFLPPASQCPLCPSRGDALTEVPADDYDVVVFENRFPSLP
                     AASGSDGRPVGRAEVVCYSSDHEASFPTLPDERLDTIAAAWVERSVALSADPGTAAVF
                     VFENRGEEIGVTLHHPHGQIYAYPFVPPRLERMLVAAQRHRDAADGACLACELLAKEL
                     REGRRILVAGSAAVAYVPQAARWPYEVHVVPRRHVGQLADLETTELAEMVRLHADALA
                     RLDQLFE"
     gene            881..1978
                     /gene="galK"
                     /locus_tag="VFV59_04510"
     CDS             881..1978
                     /gene="galK"
                     /locus_tag="VFV59_04510"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU5325126.1"
                     /translation="MVSAPDEPPDRVLARAPGRANLIGEHTDYNDGFVLPVALELQTV
                     LRGRGTPGVIRLTSDAYAGTVVIDPATGDGPMDGWGRHATAVVRALRDRGRSVRGITA
                     HVASDVPAGTGLSSSAAFEVALALTLSPEPMDAVELARLCQQAENDHVGVRSGIMDPL
                     VSAAAEAGSALFIDCRSLETERVQVPDALRILVVDSGQRRELTAGDYNRRREECEAAA
                     TALGVPALRDATLAELHSALLPEPLGRRARHVISENARVREAREALRNDDRRALGRLF
                     AESHRSLAEDFDVSTPELDALVDVARATPGIVASRMTGAGFGGCTVSLVEADAAASAG
                     SRIVAEYARRTGRTARWWVSRAGAGALSLSR"
     gene            complement(1968..2534)
                     /locus_tag="VFV59_04515"
     CDS             complement(1968..2534)
                     /locus_tag="VFV59_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325127.1"
                     /translation="MTTRTASYLAMAPDDHSSSLPVGPGAPPSFARDPEAWFGWIETL
                     LSAAPERIREIGPVEPWADREGHHWRVVATHDGPEIRPGLENPFLVTGELPIITALLV
                     TPTHIGAEVTMRRWRQVMWVPVGSVRLYHLGVAMLARYELPHEAIDRIAPGVWRDAVR
                     RWREHYALPADAHPIPASRIGARGRLND"
     gene            2621..5509
                     /locus_tag="VFV59_04520"
     CDS             2621..5509
                     /locus_tag="VFV59_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEU5325128.1"
                     /translation="MTTRPALDHRPRLHHADPDAPLTVTVYHHTHWDREWWTTRRDFS
                     VRLADLIDRLLDIIDGDDRFTTFVLDGQAVMLEDYLELRPENRDRLVRRIREGRIHVG
                     PWYVLADTLIPSGESAIRNLWLGRRVCAALDVPITPVGYLPDQFGHAAQLPQILAGFG
                     IESAVVWRGFGAPPPGQGADPNAIDLLAPPADGQPYYPRVHSRGRFPEEMQTEFWWEG
                     PDGTRVLALYLAHEYYVSHAADGALTDDAAWRGWRERQDELVGYLRPYASANRILQPY
                     GGDHLPADGRLPDLLERLNREVEADGLRFEQGSLAGFLDDVRRDADRVSVVWHGEGRA
                     FGRKAHLLPGVTSTRLYLKRLNRDAQAALERQAEPLQALSWMLGGPYEQSSLWAAWRL
                     VVQNQPHDSITGCSIDEVHRQNVARYEEAIDLGRFLALRAGERLAARLITDDLPDGAR
                     AFLVFNPLGFTRTEGARLLVDPALEVAPSTWRLADDTGATVPFQVRQVIERRTALPSR
                     LWTEVAFVATDMPSLGYRRYTLTRREEAANRPWALDHTVLGIVARDKGAARTSGLAVG
                     PARLENEHLSVLVDPPTGALTVVDQASGERYEGLNLIEDGGDAGDEYNYSWPLGDVTY
                     RATDVRPALTWLETGPARATLRITWTWRIPAGLTEDRRSRSAELVEFELHSDVTLHAG
                     VRRVDVRTHGVNPAGDHRLRARFPLGRPVETSHAESAFCVVERPVAVEQVERGSAEPA
                     VPEFPQQAFTSVDADGRGLTIANRGLPEASVLDDATGTIALTLLRAVGYLSRGDLLSR
                     IEGAGPVMPTPDAQMIGPFVAEYSIVPHAGSWRDAGSHRAAHAFNADLLAVETPGLRP
                     LTDPWRRDPNPPEPSLRPSGALLEVDGDIEVSAVKRAEERDELVVRVLNESPEPCRGR
                     IRPYRAARTAVRLDLAESPNGELAIDDGWVELELGPWELATIGLRFDA"
     gene            5506..>5956
                     /locus_tag="VFV59_04525"
     CDS             5506..>5956
                     /locus_tag="VFV59_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HEU5325129.1"
                     /translation="MSVALRGVIEGFYGRLWAPDERRRVRAAVAEAGMNVYAYAPKED
                     PFQNAEWRQPYPRTTADELERLADAGRRDGCGLWIGLRPLGISYADESDARRVVEKLA
                     SNLELGAARVILLADDISSELEAMKAGPFAALADAHAWLVTFALSALD"
BASE COUNT          805 a         2150 c         2190 g          811 t
ORIGIN      
        1 ctcgaagagc tggtcgagcc gggccagcgc atcggcgtgg aggcggacca tctccgccag
       61 ctcggtcgtc tcgaggtccg ccagctggcc gacgtggcgg cgcggcacca cgtgaacctc
      121 gtacggccag cgcgccgcct gtgggacgta ggcgaccgcg gccgagccgg cgacgaggat
      181 ccggcgaccc tcgcgtagct ccttggccag cagctcgcac gccaggcagg cgccatccgc
      241 ggcgtcccgg tggcgctgcg ccgccacgag catgcgctcg agccgcggcg gcacgaaggg
      301 gtaggcgtag atctggccgt gcgggtggtg cagcgtcacg ccgatctcct ccccgcggtt
      361 ctcgaacacg aagaccgcgg ccgtccccgg gtccgcgctc agggcgacgc tgcgttcgac
      421 ccaggccgcg gcgatggtgt cgaggcgctc gtcgggaagc gtggggaacg acgcttcgtg
      481 gtcgctcgag tagcagacca cctccgcgcg gccgaccggg cggccgtcgg acccggatgc
      541 ggcaggcaac gaggggaatc ggttctcgaa caccactacg tcgtagtcgt cggccggcac
      601 ctcggtcagg gcatcgccgc gactcgggca cagcgggcac tgactggccg gcggcaggaa
      661 cgtgcgaccc tgccgatgtg ccgcgaggat cacccactcg tccaggaggg catcgtgtcg
      721 cagctccgcc ggcggtggcc ccggctccag gcgccgccgg tccgcgggtg gctctccgcg
      781 agtgacgccg gcgtcgtcga agtagaggat ggtgcggccg tcggcgaggc ggccgcgacg
      841 cgggcgcatc ggttcaggac gtcgctagca tggggcgagt atggtatcgg cccccgacga
      901 acccccggac cgggtcctcg cgcgcgcgcc ggggcgcgcc aacctcatcg gcgagcacac
      961 tgactacaac gacgggttcg tgctccccgt ggccctggag ctccagaccg tgcttcgcgg
     1021 gcgcgggacc ccgggcgtca tccggctcac gtcggacgcg tatgccggaa cggtggtgat
     1081 cgatccggcg accggcgatg gcccgatgga cggctggggc cggcacgcca ccgccgtcgt
     1141 ccgtgcgctg cgtgaccgcg gcagatcggt tcgcggcatc acggcgcatg tcgcctcgga
     1201 tgtgccggcg ggcaccgggc tctcttcgtc ggcggcgttc gaggtggcgc tcgcgctcac
     1261 gctctcccct gagccgatgg atgccgtgga gctcgccagg ctctgccagc aggccgagaa
     1321 cgatcacgtc ggggtccgga gcgggatcat ggacccgctc gtgtcggccg ccgcggaggc
     1381 cgggtcggcc ctgttcatcg actgccggag cctggagacg gagcgggtcc aggtgccgga
     1441 cgcgctgcgc atcctggtcg ttgactccgg gcagcgccgg gagctcacgg ccggggacta
     1501 caaccgacgc cgcgaggagt gcgaggcggc cgcgaccgcc ctgggcgttc cggcgctgcg
     1561 ggacgcgacc ctcgccgagc tgcactccgc gctcctcccc gagccgctcg gacggcgcgc
     1621 caggcacgtc atctcggaga acgcgcgcgt ccgcgaagcg cgcgaggcgc tgcgtaacga
     1681 tgaccgcagg gcgctggggc ggctgttcgc cgagtcgcac cgcagcctgg ccgaggactt
     1741 cgacgtctcc acgccggagc tcgacgcgct ggtcgacgtg gcgagggcca cgccggggat
     1801 cgtcgcctcg cgcatgacgg gcgccggctt cggtggctgc acggtgagcc tggtggaggc
     1861 cgatgccgcc gcgtcggcgg ggtcccggat cgtcgccgag tacgcgcgtc ggaccggtcg
     1921 aaccgcccga tggtgggtga gccgcgcggg cgccggggcg ctgtcgctca gtcgttgagc
     1981 cggccgcgcg ccccgattct cgaggcggga atcgggtggg cgtccgcggg cagggcgtag
     2041 tgctcgcgcc agcggcggac ggcgtcacgc cagacgcccg gggcaatccg gtcgatggct
     2101 tcgtgcggca gctcgtatcg ggcgagcatg gcgacaccga ggtggtagag gcggacgctg
     2161 cccaccggga cccacatgac ctgccgccag cgccgcatgg tcacctctgc gccgatgtgg
     2221 gtcggcgtga cgagcagcgc cgtgatgatc ggcagctcgc cggtgaccag gaaggggttc
     2281 tccagtccgg gccggatctc cggaccgtcg tgcgtcgcga cgacgcgcca gtggtgtccc
     2341 tcgcgatcag cccacggctc caccgggccg atctcgcgga tgcgctccgg cgccgcactg
     2401 agcagggtct cgatccagcc gaaccaggcc tcggggtccc gcgcgaagct cggcggagcg
     2461 cccgggccga ccggcaggct ggaggagtgg tcgtcgggcg ccatggcaag gtacgacgcg
     2521 gtgcgggtgg tcacaagggg agatgcctga gcaatcgtcg tgccgcttct cgtgcctttc
     2581 ggccgctcct tcggagaagc ggtcgcctag gatcgaggcg atgacgactc gacctgccct
     2641 cgaccaccga ccacgcctcc accacgccga tcccgacgcc ccgctcaccg tcaccgtcta
     2701 ccaccacacc cactgggacc gcgagtggtg gacgacacgg cgtgacttca gcgttcgcct
     2761 cgccgatctg atcgatcggc tgctcgacat catcgacggc gacgatcgat tcaccacctt
     2821 cgtcctggac ggccaggccg tcatgctcga ggactacctc gagcttcggc cggagaaccg
     2881 ggatcgcctg gtgcgacgga tccgcgaggg gcggatccac gtcggtccgt ggtacgtgct
     2941 ggcggacacg ctcatcccca gtggcgagtc cgccatccgc aacctgtggc tcggacgccg
     3001 ggtgtgcgcg gccctcgacg tgcccatcac gccggtcggc tacctccccg accagttcgg
     3061 ccacgcggcg cagctgcccc agatcctggc cggcttcggg atcgagagcg ccgtggtctg
     3121 gcgtggcttc ggggcgccgc cgccgggcca gggcgcggac ccgaacgcca tcgacctgct
     3181 ggcgccgccc gccgacggcc agccgtacta cccccgcgtg cactcgcgcg gccggttccc
     3241 cgaggagatg cagaccgagt tctggtggga gggccccgac ggcacccgcg tgctggcgct
     3301 gtacctggcg cacgagtact acgtcagcca cgcggccgat ggcgccctga ccgatgacgc
     3361 cgcctggcgt ggctggcgcg agcgccagga cgagctggtc ggctacctgc gcccgtacgc
     3421 cagcgcgaac cgcattctcc agccctacgg cggtgaccac cttccggccg acggccgtct
     3481 ccccgatctg ctcgagcgac tgaaccggga ggtcgaggcc gacggtctgc ggttcgagca
     3541 ggggtcgctg gcgggcttcc tggacgacgt gcgccgtgat gcggaccgcg tctcggtcgt
     3601 ctggcacggc gagggccgcg ccttcggccg caaggcgcac ctgctgcccg gcgtgacgag
     3661 cacccggctg tacctgaagc ggctgaaccg ggacgcgcag gccgcgctgg agcgccaggc
     3721 ggagccgctc caggcgctct cctggatgct cggcggcccg tacgagcaga gctcgctgtg
     3781 ggccgcctgg cgcctggtcg tgcagaacca gccgcacgac tcgatcaccg gctgctccat
     3841 cgacgaggtc caccgccaga acgtggcgcg ctacgaggag gccatcgatc tcggccgatt
     3901 cctggccctg cgcgccggcg agcggctcgc cgcgcgcctg atcaccgacg acctgcccga
     3961 cggcgctcgg gccttcctcg tcttcaaccc gctgggcttc acccggaccg agggggcgcg
     4021 gctgctggtc gacccggcgc tcgaggtcgc gccgtccacc tggcgcctcg cggacgacac
     4081 cggcgccacc gtcccgttcc aggtacggca ggtgatcgag cgacgcacgg cgctgcccag
     4141 ccggctgtgg accgaggtgg cgttcgtggc gaccgacatg ccgtcgctcg gctaccgccg
     4201 ctacaccctg acgcgtcgcg aggaggcggc caaccgcccg tgggcgctgg accacaccgt
     4261 cctcggcatc gtggcgcgcg acaagggtgc ggcgcggacg agcggcctcg ccgtcgggcc
     4321 ggcacggctc gagaacgagc atctcagcgt gctggtcgac ccgccaaccg gcgcgctgac
     4381 cgtcgtggac caagccagcg gcgagcgata cgagggcctc aacctcatcg aggacggcgg
     4441 cgacgccggc gacgagtaca actactcgtg gccgctcggg gacgtgacgt accgcgccac
     4501 cgacgtgcgg ccggccctca cctggctcga gaccggtccg gcccgcgcca cgctgcgcat
     4561 cacgtggacc tggcggatcc ccgccggact caccgaggat cggcgcagcc gttcggccga
     4621 gctggtcgag ttcgagctgc actcggacgt cacccttcac gccggcgttc ggcgcgtcga
     4681 tgtccggacg cacggcgtca acccggccgg cgaccatcgc ctgcgcgccc gcttcccgct
     4741 gggccgcccg gtggagacgt cgcacgccga gtccgccttc tgcgtggtcg agcgccccgt
     4801 ggcggtggag caggtggagc gcggcagtgc cgagccggcc gtgcccgagt tcccgcagca
     4861 ggccttcacc agcgtcgacg ccgacggccg ggggctcacc atcgccaacc gcgggctgcc
     4921 ggaggcatcg gtcctggacg acgcaaccgg gacgatcgcg ctgaccctgc tgcgcgccgt
     4981 cggctacctg agccgcggcg acctgttgag ccggatcgag ggcgccggac cggtcatgcc
     5041 aacgcccgat gcgcagatga tcggtccgtt cgtggccgag tacagcatcg tgccgcacgc
     5101 cggcagctgg cgcgacgccg gcagccatcg cgccgcgcac gccttcaacg ctgatctcct
     5161 tgccgtcgag acgcccggcc tgcggccgct gaccgatccc tggcgccggg atccgaaccc
     5221 gcccgagccc agtctgcgcc cgtcgggcgc gctcctcgag gtcgacggcg acatcgaggt
     5281 gagcgcggtc aagcgcgcgg aggagcggga cgagctggtg gtccgggtgc tgaacgagtc
     5341 gcccgagccg tgtcgaggac gcattcggcc gtaccgggcg gcgcggacgg ccgtccgtct
     5401 cgacctcgcc gagtcgccga acggtgagct ggcgatcgac gacggatggg tggagctgga
     5461 gctcggtccc tgggagctgg ccaccatcgg cctgcgcttc gacgcgtgag tgtcgcgctg
     5521 agaggggtga tcgagggctt ctacggtcgc ctctgggcgc ccgacgaacg ccgtcgggtt
     5581 cgggccgcgg tcgcggaggc agggatgaac gtctacgcct acgcgccgaa ggaggacccc
     5641 ttccagaacg ccgagtggcg ccagccgtat cccaggacca cggctgacga gctggaacgg
     5701 ctggcggacg cgggccgacg ggacggctgc gggctgtgga tcggactgcg gccactgggc
     5761 atctcgtacg cggacgagtc ggatgcccgc cgcgtggtcg agaagctggc gagcaacctc
     5821 gagctggggg ccgcacgcgt catcctgctg gccgacgaca tctccagcga gctcgaggcc
     5881 atgaaggccg gaccgttcgc cgccctggcc gatgcgcacg cctggctggt gaccttcgcc
     5941 ctgtccgcgc tcgacc
//
LOCUS       DASTYP010000095         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_334324, whole genome shotgun sequence.
ACCESSION   DASTYP010000095 DASTYP010000000
VERSION     DASTYP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334324"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="VFV59_04530"
     CDS             complement(<1..1898)
                     /locus_tag="VFV59_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5325130.1"
                     /translation="MTNQRRGPEERRVDPLLEVRERRRGKRMGDAYVKIVRPFEAEFQ
                     RGDEGELIATERTLLRRSGWKAALRGFRTWLIGRPIHREREEHERLTKVKALAIFSSD
                     NISSSAYGPEEIMRVLAGASAGAIMLTLPLAALIILMLAIVTLSYRQTIKAYPHGASS
                     YIVASDNLGPFLGVLAAAALLIGYVVTVAVSVSAGVLAMTSIIPALYPERVLIGVVAV
                     GVLMLGNLRGIRESGTIFMVPTYAYIVVMLGIIGYGVVRTVLGDVPPFEAPAAWEAVQ
                     ESGQVLGLFLILRAFSQGAVALTGVEAISDGVPAFKSPEWRNARTTLTWAAAIFAVLF
                     LGIAFLVTTMGIVPDPTERQTVLSLLVRHLTGNGPVLVIAQIATALILVLAANTSFAD
                     FPRLSYFLARDEYLPRQFAFRGERLAFTTGILFLSIMAAGLIIGFDASVAGLIPLYTL
                     GVFIAFTLSQTGMLARWWNRREAGWRSGLAINGLGAATTAVVALVVGSSNFFAGSWLV
                     IVLVPILMAVLMAIHRHYRDLNAALAVERIPLADEVAARPIVVVPIARLDRPALQAIA
                     FANSLSADAVAVHVTNDASEAEQLREQWPSWAASTNLVIVESPYRALVGPLMAYLDAI
                     QQQAPERP"
     gene            complement(1993..2826)
                     /locus_tag="VFV59_04535"
     CDS             complement(1993..2826)
                     /locus_tag="VFV59_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325131.1"
                     /translation="MDELRNEARAAVAIARKELRVALRYRLNAINQVLQPLYQILIPG
                     LLLGATFAIGGQNVGLAKTAGTTDVVGYLFLGMLALNLTAAGFWGVAYSIKMELDAGT
                     LEPAWLTPTRRETFVVGMTLAGLAMAVVSTAILVLIGIWLFGADLGPRLVAAVPILAV
                     TSFGILGIGYLIGSVVLRMRESNFFVDAFDFIFIALSGATFPIVVLPGAVQMVSYALP
                     TTHALDLMRVAALGTPPLLPLAAEWVLLVVTSLALLVVGRWAWLSTERRLRVRGTLGQ
                     Y"
BASE COUNT          517 a         1038 c         1050 g          379 t
ORIGIN      
        1 atcggccgtt ccggagcctg ctgctggatg gcgtcgaggt aggccatcag cggcccgacc
       61 agggcccggt agggcgactc gacgatcacc aggttggtgg aggcggccca cgacggccat
      121 tgctcgcgca gctgctccgc ctcggaggcg tcattggtga cgtggacggc gaccgcatcg
      181 gcgctgaggg agttggcgaa cgcgatggcc tggagcgctg ggcggtcgag gcgcgcgatc
      241 ggcaccacca cgatggggcg ggccgcgacc tcgtccgcga gcggaatgcg ctccaccgcc
      301 agcgcggcgt tcaggtcgcg atagtggcgg tggatggcca tgaggacggc catcaggatc
      361 ggcacgagca cgatgaccag ccatgagccc gcgaagaagt tgctgctgcc gacgaccagc
      421 gccaccacgg cggtcgtggc ggcacccagg ccgttgatcg ccaggccgct gcgccagccc
      481 gcctcacgcc ggttccacca tcgggcgagc atgccggtct gcgacagggt gaaggcgatg
      541 aacacgccca gcgtgtagag cgggatcagg ccggcgacgc tggcgtcgaa gccgatgatc
      601 aggccggcgg ccatgatcga gaggaagagg atgccggtcg tgaaggccag gcgttccccg
      661 cggaatgcga actggcgcgg caggtactcg tcgcgcgcca ggaagtagga gaggcgcggg
      721 aagtcggcga agctcgtgtt cgcggccagc accaggatca gtgccgtcgc gatctgcgcg
      781 atgaccagca ccggtccgtt gccggtcagg tgccggacca gcagcgagag gacggtctgg
      841 cgttccgtcg ggtccgggac gatgcccatg gtcgtcacca ggaacgcgat ccccaggaac
      901 agcaccgcga agatggccgc ggcccacgtg agcgtggtcc gcgcgttgcg ccactccggc
      961 gacttgaacg cgggaacgcc atcggagatc gcctccacgc cggtcagcgc caccgcgccc
     1021 tggctgaacg cccgcaggat gaggaacagc cccagcacct ggccgctctc ctggaccgcc
     1081 tcccaggcag ccggtgcctc gaacggcggc acatcgccga ggacggtgcg caccacgccg
     1141 tagccgatga tgccgagcat cacgacgatg taggcgtagg tcggcaccat gaagatggtt
     1201 ccggactcgc ggatgccgcg caggttgccg agcatcagga cgccgaccgc caccacgccg
     1261 atcagcaccc gctcgggata cagcgcgggg atgatcgagg tcatggccag cacgccggcc
     1321 gacaccgaca ccgcgaccgt caccacgtag ccgatgagga gcgcggccgc ggcgagcacg
     1381 ccaaggaacg ggccgaggtt gtcgctcgcc acgatgtagc tcgaggcgcc gtgcgggtag
     1441 gccttgatcg tctgccggta cgacagcgtg acgatggcca gcatcaggat gatcagcgcg
     1501 gccagcggca gcgtcagcat gatcgcgccg gcactggcgc ccgccagcac gcgcatgatc
     1561 tcctcgggcc cgtaggccga tgagctgatg ttgtcgctcg agaagatggc gagcgccttg
     1621 accttggtca gtcgctcgtg ctcctcccgc tcacgatgga tgggtcggcc gatgagccag
     1681 gtgcggaacc cgcgcagcgc cgccttccat cccgagcgtc gcagcagggt ccgctccgtg
     1741 gcgatcagct cgccctcgtc gccacgctgg aactccgcct cgaaggggcg gacgatcttc
     1801 acgtacgcgt cgcccatccg cttcccgcgg cggcgctcac gaacctcgag gagcgggtcg
     1861 acccgacgtt cctcggggcc tcgacgttgg ttggtcacgg cgccgaacgg tacaccacgc
     1921 gccgggatga cggggcggga cgcatcgagt ggagagcacc gcaacccgcg tgccgaccgg
     1981 tggccgcgcc gctcagtact gccccagcgt cccccggacc cgcaggcgcc gctccgtcga
     2041 cagccacgcc cagcgtccca ccaccagcaa cgcgaggctc gtcaccacca gcaggaccca
     2101 ctcggcggcg agtggcagga gcgggggagt gcccagcgcc gcgacccgca tcaggtcgag
     2161 cgcatgggtc gtcggcaggg cgtacgacac catctgcacc gcgccgggca gcacgacgat
     2221 cgggaacgtg gcgccggaca gcgcgatgaa gatgaagtcg aacgcgtcaa cgaagaagtt
     2281 cgactcgcgc atgcggagga ccaccgagcc gatgaggtag ccgatgccca ggatcccgaa
     2341 cgaggtcacg gccaggatgg gcaccgccgc caccaggcgc gggcccaggt cggcgccgaa
     2401 gagccagatg ccgatcagca ccaggatcgc cgtgctcacc accgccatgg ccaggccggc
     2461 gagcgtcatg cccacgacga aggtctcacg gcgggtcggg gtgagccagg ccggctccag
     2521 cgtcccggcg tcgagctcca tcttgatcga gtacgcgacg ccccagaatc ccgcggccgt
     2581 caggttcagc gccagcatgc cgaggaagag gtagcccacg acgtcggtcg tgcccgccgt
     2641 cttcgccaac ccgacgttct gcccgccgat cgcgaacgtc gcgcccagca ggagcccggg
     2701 gatgagaatc tggtacagcg gctggagcac ctggttgatc gcgttgagcc gatatcgcag
     2761 cgcgacccgc agctccttgc gcgcgatcgc cacggcggcc cgcgcctcgt tgcgcagctc
     2821 gtccatcagt actgggccaa ccgcccgccg cggcgggcgt actgctccgt tcgccggaag
     2881 gcgatgacgg ccaggatggc cagcacgacg cccatgccgg ccaggatggc caggtcgccg
     2941 atgacctggc cgacctcagc gccggtcagc agcacctgcc gcag
//
LOCUS       DASTYP010000096        34891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_589719, whole genome shotgun sequence.
ACCESSION   DASTYP010000096 DASTYP010000000
VERSION     DASTYP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 34891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34891
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589719"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFV59_04540"
     CDS             <1..390
                     /locus_tag="VFV59_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325132.1"
                     /translation="EQGAVGAVVARANVTVTQAGARTLIAGERLEVHQGGGAMLLSGG
                     PVEVTMGGFGTALAAGGMRFERGGAMVAIAPSIDVGENSGVGIALTPRLNLAPGARVV
                     LGLREAAVIGAVAGGVIGTIIALLRRR"
     gene            complement(461..895)
                     /locus_tag="VFV59_04545"
     CDS             complement(461..895)
                     /locus_tag="VFV59_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5325133.1"
                     /translation="MIAGAPGTTRPEELRVADLMTIDPVTVAVDASIEEAEDLLRHHR
                     ITGLPVVDADGLLVGVISLTDLLYLAQPTVGALIRGRESGIRVGEVMSVPPITIDTTA
                     TVRDAARAMDADRLHRLVAVDAQGRPVGVISSMDFVSLVAEG"
     gene            complement(892..1386)
                     /locus_tag="VFV59_04550"
     CDS             complement(892..1386)
                     /locus_tag="VFV59_04550"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU5325134.1"
                     /translation="MVTSTPVIDERLENWLHAHAITFEVHPHPLTFTALATARAEGIE
                     PRTFAKVVGVRTEEGRVALIVVDAVDVLDLAKVRRLIGEPVRLLSELELTAACPAWEV
                     GTVPPVPTLAGVQVFADEAVRSDAQISFNAGSHRVAVRVDRAAWEEAAEIAYGDLVRR
                     RTDR"
     gene            complement(1411..2151)
                     /locus_tag="VFV59_04555"
     CDS             complement(1411..2151)
                     /locus_tag="VFV59_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5325135.1"
                     /translation="MKVSDVMTRGVVCATPSMRLRDVARLLSEHQVSGLPVVDEGGGR
                     IVGVISEGDLLAKQLTHPLSRRLPLEWILGEHHDPDELRRRAATTAGEAMTSPALTIE
                     ADRPVREAAARMIERRVNRLPVVAGGRLVGIVTRADLVRAYLRLDDEVRHAVTEDVIR
                     RTMWLDPSRFDIDVDEGIVTIAGTVDRRSTARILAKLIGLVDGVTGLHDQLRWDFDDS
                     SIEPGGETEREPGAASVTAREHPPALHR"
     gene            complement(2267..3148)
                     /locus_tag="VFV59_04560"
     CDS             complement(2267..3148)
                     /locus_tag="VFV59_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5325136.1"
                     /translation="MNILLALDGSPSSLVARDLVAGLSWPEATTVDLLAAYQTPIDWS
                     GGMAPADWMGEVDEATRGQLAEDLEAAAAPLLQHGLAVERHVVAGRPATEIVAAAHAR
                     DAALVVMGSRGRGPLRSMLLGSVAGEVTAHAACPVLVARHPTVTRLVVATDGSAGAEL
                     IPDRLAALGAFTGVPADVVAVSIPDTPTFELIVGLYTLGDERLSHRRQELRQRFAAAA
                     DRMVERLTAVGIPATGHVRAGDPAHEVLALAREREADLVVTGSRGLGALDRLLLGSVA
                     RNVLHHATASVLVVGHP"
     gene            complement(3256..3636)
                     /locus_tag="VFV59_04565"
     CDS             complement(3256..3636)
                     /locus_tag="VFV59_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325137.1"
                     /translation="MNDERSGHDLERDSTGDAVGPAQGGGPPDALPWTLVVEDEGWSA
                     DLEHGVQLPCTGDRIEYITEHGERRMYVVDAVVHTLQRSSSERPPVADAERGPNTLVD
                     AGANPEPPGLLRAGLPRVVVHRAD"
     gene            3711..5075
                     /locus_tag="VFV59_04570"
     CDS             3711..5075
                     /locus_tag="VFV59_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5325138.1"
                     /translation="MELHEDQVARGRRERWWRSTFVIIATVYVSLLLIGLLLDILSGF
                     AQIALTLFIAWLLAFVLSPLVSWMVTRFRLPRGLAVGIVFGAALVIGAAFIFYAAASV
                     AANVGDMTAEFPLTRARIEGVLREWQDAITFGRFAPDLVQVYRDAERSLVGVAGPILG
                     EVPQVGASVLGSLALVIILSVYMLADSAGILAKVNRVIPSRYEDEVEILERTISRAFG
                     GFLRAQVLLAAIQTVLTILVVILVGLPYGFLITAASALGMLIPFFGPPLALIPPIVAT
                     AIFASDWIIPVAGGLLIVQTVLVNYLQPRLMQEALGMHPLLVLVGLLIGAQVAGLWGA
                     LFGIPVIAVLNVFFNYVVNLRTLEESPEETEEVLEEVRAEAPDAPPEELVAMAADRVE
                     AEEAEEEAEAAESMDAASDDLRAAAGELRAAAGEQRQAAGQIGESAAELREASERLAE
                     DRPS"
     gene            complement(5072..5452)
                     /locus_tag="VFV59_04575"
     CDS             complement(5072..5452)
                     /locus_tag="VFV59_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5325139.1"
                     /translation="MTPKSHSILVVDDDSMVLSLYVDALAESGHRVEVAPNGEAALRR
                     LQTGPIPCVVLTDVRMPVMDGFELSSALAADPQLASVPVVMLTGDRMLSFTSPARDKP
                     FDPAELDALVQRTCVLHRRQEGAA"
     gene            5694..6701
                     /locus_tag="VFV59_04580"
     CDS             5694..6701
                     /locus_tag="VFV59_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU5325140.1"
                     /translation="MSATAITSSRRVVARTLGLGISALLTAGLLAWTPTAVSGWNQGS
                     AEATLWQLMNGARVNNGIAPVQSNGTLVALARWRSADMLQSNYFSHTIPSCGCLVYTF
                     YDRNGLSYDWAGENIGWNSGLDDAYSPVRVHEAFMASAGHRANVLDARFTHGGVGAAA
                     ADGQMFQGYVQNTRMYTELFMQARYSAPAPAPAPQQPAPAPRPPSGGGSGGGGGGGGA
                     TAPRTTTALRPAATPQPTPKIVSMAAPRRPSSAAGIDGVAQVVNARPPSVAALVTRFT
                     VDELDRPATGRWTAAAPARDGKDAASAALTRVPLQVAAAPVDDGGFGGFLGAIIGFLF
                     G"
     gene            6770..7516
                     /locus_tag="VFV59_04585"
     CDS             6770..7516
                     /locus_tag="VFV59_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325141.1"
                     /translation="MPVILEARDLVKSYPVGDGHVTALDGVSLAVERGEFVAIMGSSG
                     SGKSTMLHLLGGLDLPSSGEVVIDGVRISALDEERRTLTRREKTGFVFQFFNLVPTLT
                     VAENVALPFLIAGEQLSRHRERVDELLALVGLTDKAAYRPDQLSAGEQQRVALARALA
                     TEPAILLADEPTGNLDYTTGTEILDLLWDSADRLGQTIVLVTHDARAAAYADRVLVVR
                     DGRIRDEIALGRRADHEARGLITRLAELGL"
     gene            7525..10062
                     /locus_tag="VFV59_04590"
     CDS             7525..10062
                     /locus_tag="VFV59_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5325142.1"
                     /translation="MRLATLAWRGLLARPLRTALTVAGVAFGVAVLAATIVIGSSSER
                     ALQGLSADLLGRADVRLRAFEEAGFTPRTLQAIRADPNVVAAAPVAQRRLTAYTAPGE
                     GERVFSVMALGIEPAADAEVRPPSVVSGRALDPDAADEVLVPAAFAARNGLGLGSEVR
                     LDGRREGLPPLRIVGLLRDTGLGAVEGGDVIVLARSTLDDSFAVPAPVRAVDVDLGEN
                     PTSAEIDRLTALLDEPYVVETPEDAARGVAGAQTAFGQVALLFGLVALVVGAFLVGNT
                     LAMTVSERTRELGLLRAAGTTSRQVLGIVLRQAAAIGLVGSAAGVLLGLLLATLLAGL
                     VSSTRAVLLTGLVVPPVGMIVAFLLGLAATLLGAAFPAARAARLSPLDALRPSRRPER
                     GLLERLRPLILLEVAAVLIGLVVTVVVGGGSPVLPILLSLALLIGGALAAAFVLEPLG
                     RLIGRPFEWFFGAQGLLGRASLTRDRARTGLTVGAMMIALAAVVALGTVAESARAGTE
                     RWVDSILPGGHAIRTSLPLDVETFRPTIEATAGLRVASPLLEVPVIRVDGDTREEAIV
                     VGIDPNVFQDNRALVIRDGRRAAAFSALRAGGGVLVPAALADRAGIAAGDTLLLGVPG
                     GQAHEMTVAGVVEYTLPARTPDGALLVGSADARDLFGATTASLWVLVPQPTIGDAAFA
                     SAVRETALQLAAEPLTAADLARDLSRSLDSLTGLLDVLALVAVAIAALGIVNTLGIGV
                     QERIREIAILRSHGMTVGQVQAMIVTEATIMGAVAGVLAVATGVVVASALVNGGVATQ
                     LDAGVRLPWALLIAVVLTGAGIAALSGLYPARAAAKLPIVGSLKHFE"
     gene            10071..10499
                     /locus_tag="VFV59_04595"
     CDS             10071..10499
                     /locus_tag="VFV59_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEU5325143.1"
                     /translation="MATRFWEARAGRAFVGRLETGSDLVEEIERFCLEQSVMAAQVTV
                     IGAVRRARYAYYEQDDHRYRELETDTHHEITGFVGNISLKDDRPFLHAHATFADADGA
                     TVGGHLLRGIQVFAAEVMIREMGGVTLTRMHDEETGLSLW"
     gene            10565..10699
                     /locus_tag="VFV59_04600"
     CDS             10565..10699
                     /locus_tag="VFV59_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325144.1"
                     /translation="MPAILIWVLWIVVSIVLFDVLFVGARVLATRAWEVERPVRRRRR
                     "
     gene            complement(10721..11167)
                     /locus_tag="VFV59_04605"
     CDS             complement(10721..11167)
                     /locus_tag="VFV59_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325145.1"
                     /translation="MTRVLVVYHDVDVADIEVDELRRAGYEVDQCAGPIGGDPCPVAS
                     GQPCWQVEQADVLVYDIWEGAPGHADLIADVRRVHPDKPIVLTSSGSILDALENNPEL
                     SDVPVLHAPTRASLLQAIERAVATGPVPQRVPQHEDRRPAFSGPRW"
     gene            11383..11847
                     /locus_tag="VFV59_04610"
     CDS             11383..11847
                     /locus_tag="VFV59_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325146.1"
                     /translation="MRRVGGQEVSGTAGFRITEEGPAPPWIPRFDRDPIVWLQAVRRW
                     RLGPTWMRVVATLDPGEWISTAGHRWRMLRTVRGVEIRPDLVAPFLHFGSFPIVTALG
                     IGVAVEVELTTESFRWVDVFAPEQFDFAELGRAMLEERQLPHDNLPLLFPAR"
     gene            11910..12680
                     /locus_tag="VFV59_04615"
     CDS             11910..12680
                     /locus_tag="VFV59_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325147.1"
                     /translation="MTAAESSRRGTQPAVVARGLTRQYGRVRAVDGIDLDLPPGRIYG
                     LLGPNGSGKTTLIRLLTGLARPTSGSASVAGVQMPSRANLARIGYMTQADGVYPELSV
                     WENLRFFARLYGPIERSALTESLRLVELDAAVDRPADRLSGGMRRRLSLACALVHDPE
                     VVFLDEPTVGVDPALRVSFWEHFRALADAGRTLLVASHVMDEAERCDVLLFIRAGRVV
                     ASGSADDLRRRAGTPNLEEAFLRFAGEAGVGSAEELAP"
     gene            12677..13834
                     /locus_tag="VFV59_04620"
     CDS             12677..13834
                     /locus_tag="VFV59_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325148.1"
                     /translation="MNLRRVASLTRRIVDQFRRDPRTLALLFVAPIFIMALLGWVLRG
                     QANPQPRVAVVNEAGTSAEPVVDAIEAALADEGGSIVGVGSREADARQAIDERRIDVA
                     LVVPEGFGAGGGELLVITPGLNPPAEGANVLAVQQAIAAAVRSSVPPAMAGLVPEVRH
                     EALYGPADADQLDTLAPVFVGYFAYFFVFILTGISFLRERVGGTLERLMATPITRGEI
                     VLGYSIGFGIFATIQVALVLAFTVLRVDVPAVGPVPAWWFGLGVPVAGSPLLAYLVAL
                     VLGLGAVSLGIFLSTFARSELQILQFIPIVIIPQGLLGGIFWPIETLPSVLEPVARAL
                     PITYAVEGLREVFIAGAGLDSPTVQLDLAVLAGIAALFVVLAAATIRREVA"
     gene            complement(13844..14335)
                     /locus_tag="VFV59_04625"
     CDS             complement(13844..14335)
                     /locus_tag="VFV59_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5325149.1"
                     /translation="MDDTDALREQLTRLLRGQDAHMDFLEAVADFPDEAVNDRAPNVT
                     YTPWQLAEHVRITQWDILEYVRNPSHVSPDWPIGYWPDPEATATPEAFATTVAGFTRD
                     REALLAIVADASVDLLAPMAHAPRHSVGREIRVVADHTAYHTGEFAILRQVMGTWPAA
                     REG"
     gene            complement(14516..15787)
                     /locus_tag="VFV59_04630"
     CDS             complement(14516..15787)
                     /locus_tag="VFV59_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325150.1"
                     /translation="MRWLLEGAAPARLGPGFRWLLASSWTTNLSDGIAIAAGPLLVAS
                     QTEDAFLVAMAALVQWLPPLLFGLYAGALSDRLDRRLIVVTVNLVRGAVLAVIAASVL
                     TDTISVAVVLGALFVLGTAEVFADNTTHTLLPMLVRRDDLATANSRLMAGFVTVNQLA
                     GPPLGAALFALGMALPFVGQALLVVAGAALVSRIALPPAASERRETAIRHDIAEGVRW
                     VRHHAAVRTLVLTIFTFNITFGAAWSILVLYATQRLGLGEIGYGLLTTISAAGGLVGT
                     FSYGWITRRVSLGNLMRIGLIIETLTHLGLAVASVPWVAMVIFFVFGAHAFVWGTTSI
                     TVRQRAVPTELQGRVGSVNTVGTFGGLVIGSALGGVLAQSMGVTAPFWFAFVGSAIFV
                     VLIWRELRHIAHADEAMVDSDLTWMAAADAD"
     gene            15918..16160
                     /locus_tag="VFV59_04635"
     CDS             15918..16160
                     /locus_tag="VFV59_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325151.1"
                     /translation="MTDLELELAALRRRRRSPGFAAWLRQFAPFDRRAIRDPDERDAY
                     WVTVQQRLVESDAQYLMNSRATEPPVRTDLPLLIGG"
     gene            complement(16234..16416)
                     /locus_tag="VFV59_04640"
     CDS             complement(16234..16416)
                     /locus_tag="VFV59_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325152.1"
                     /translation="MEWIIVIVLVVLLLASVGPRAGYYGTASPLYDVLSLLILIGLIV
                     FVLDLLGVINVFGIGA"
     gene            complement(16599..17006)
                     /locus_tag="VFV59_04645"
     CDS             complement(16599..17006)
                     /locus_tag="VFV59_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325153.1"
                     /translation="MLERLVAAAAIFLAANFVMSYIIPAPAPSPWWLVFPFGREGAQA
                     TWTFGRIDGFPLVLMLAVAGLTVLAFIGAFLATFGIWVAADLWRALVIVGASGSLALF
                     LLHLGPWAVAPLALDLLLLWVALSAVWTPSSAT"
     gene            complement(17037..17480)
                     /locus_tag="VFV59_04650"
     CDS             complement(17037..17480)
                     /locus_tag="VFV59_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU5325154.1"
                     /translation="MTGVAVRDVVSLPASHLDLLTRPICGVLTTMGAEGQPQSSLVWV
                     DHDGSCARVNTTLERRKGRNLVSNPKVSLLVVDPTNTGRFIQMRGDAELVFAGAEEHL
                     DALTRKYTTHPRFYGWVYPAEQRARETRVICRIHARRVTLDAIHA"
     gene            complement(17477..17950)
                     /locus_tag="VFV59_04655"
     CDS             complement(17477..17950)
                     /locus_tag="VFV59_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU5325155.1"
                     /translation="MRVTGLVTGARCTTIPPSHLDLVDAPPVAALSTIGADGYPQTSV
                     VWCDFDGECVRVNTMRGFAKERNLRRDPRATLLCYDPAEPLRYLEVRGTVVEMTEVGA
                     LEHLDALASRYAGRPIRYFGDAIPAEFASTETPVLCRIRPTHVVAIDATAGGGSR"
     gene            complement(17962..18363)
                     /locus_tag="VFV59_04660"
     CDS             complement(17962..18363)
                     /locus_tag="VFV59_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325156.1"
                     /translation="MLKILIALVLMAHGIGHSMGPLGMFNIATINPAWHGDSWILSGV
                     GPSLTQVVGTVLWTLALVGFIAVGAVVLGWLPADWWVPLAVGSSLVSIAGIILFPVAF
                     PVFSTLGALAVDVVVLAAALWLGWTPSDVAV"
     gene            complement(18363..19259)
                     /locus_tag="VFV59_04665"
     CDS             complement(18363..19259)
                     /locus_tag="VFV59_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5325157.1"
                     /translation="MPTITIRGVPLYVEVVGKGPPLVLMHGGPGLDHISLTPFRALAD
                     RHTVVLYDHRCNGRSIGAPITSMTFDNLTADADALREHLGFERWDVLGHSFGGHVALE
                     YVLRYPERVSHLVLLDTAGDARWSQERAADVLAERGYDRKTVAVARRFYSGRVAPKDF
                     VRASFRLLPAYDSRFSLLKLARELAAGGWRMQTRPEALMFGGRMMRGWTVMDRLGEIR
                     APTLVMAGHDDFLFPPESQSMLAAGIPDARLRIIQRAGHNPQAERPTETLEAVTAFLD
                     DAALQPVEPAYRPLAAAARGAP"
     gene            19486..21006
                     /locus_tag="VFV59_04670"
     CDS             19486..21006
                     /locus_tag="VFV59_04670"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5325158.1"
                     /translation="MHREVAPAVAAIAHRHPAVGLAVGVVSDGGLACFAGHGLADIAS
                     GTPVTKDTVFRIGSITKTLTGVAVMQLWEQGLLDLDAPVNRYLRAFPLVADREDWRHV
                     TVRHLLTHTAGVPDVRSVRDLLRGGMTPADGRPPVLSVPFGEALPSLADHYADGLRVV
                     AEPGTAWAYSNPSFAALGQVVEDVTGQPLDRYLRQHVFAPLGMEHSDLVRSDRVASRL
                     ATGYAMGPHGASPVQDRDWIGVGAGGAYATSGDLALYLAALLGGGANEHGRVLRASTL
                     ATMLEPQFQPDPRVPGMGLSFFRGDLGGHRVVYHDGILPGFNTSLVAAPDDGIGVFGM
                     TNGSAGAFAWLQVELDALTRHLLGIGDEDRHAPHHPELWPAFCGRYVLPPGVADVRER
                     LMLGGGIEVLVRGGRLLARVLTPIPILFRGFPLEPADRDPDVFRVDLSSLGMAPVRVV
                     FGTRAGGRAMALHTDVGGQPWSLRRAPAAPGWRGPAVRAGLAAAAATAVLWRRRRV"
     gene            21009..22427
                     /locus_tag="VFV59_04675"
     CDS             21009..22427
                     /locus_tag="VFV59_04675"
                     /inference="COORDINATES: protein motif:HMM:NF005687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5325159.1"
                     /translation="MAVSTATELWRLGAVELATLIASRQASCVEVVDAHLRRIQAANT
                     EINAVVIVLAEEALEAAAAADRAIAPGAALQPLHGVPFTVKQNIDVAGTPTTQGYRAL
                     SNAYPARDAPAVERLKAAGAIVLGRTNLPTGAMRWHCESELWGVTLNPWDRSRTPGAS
                     SAGEAAAIATGMSPLGLGNDGLGSLRHPAQCCGVTALKPTLGRVPQDSTVASGDAGPI
                     GIQLTGVTGPLARRVADLRQAFHVIAGPTWRDPWSVPVGFGGPDLAPPIHVAVVLDPG
                     GLGTAAPVQDGVRAAATALADAGYAVEEREPDGIDAAATALVVMDSTPGIRAVWERMA
                     ATAPDPVRRFATAFFASAGEPDGLAVEESFVVRHAVRRAWGQFQESHPLIVAPIATEM
                     PSQAGTDLDEGHVAADIRAMRMATAVNALGLPAVAVPVGVADGLPLAVQVIGPPYRED
                     LALDAAQAIEDRLGVLTPIDPR"
     gene            22438..23835
                     /locus_tag="VFV59_04680"
     CDS             22438..23835
                     /locus_tag="VFV59_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEU5325160.1"
                     /translation="MGPIERLSVLDQLMLWASRRWPQHIGALLILEGEPLLDAAGELR
                     IQELRERVASRLHLVPRFRQLILRPRRGLGGPLWVDAPAFDVARHVRQMRLEPPASEA
                     ELVAAIEGLLLAPMDPGQPLWDMRFVVGLPERRIAVLLRIHHSIADGMAAMRTVTRFL
                     DSSPEAPTQAAPPWRPDAPPSSRALLGDNVRRRLRGIGSSLAPLTRPRATLRTVRAAW
                     PAIREILAEEPATATSLDRMVGERRRIALIRSDLSTVRAIGRRHDATVNDVLLSITTA
                     GMREVLLARGEAVDGTTIRIYVPVSLRRHLHGRQEGNLIAQMAVPLRMGLADSERRLD
                     AIAAETRQRKLRARTSLSNLMVGGTIGRRLLLFAVMRQRVNATSASIPGPKQPLYLAG
                     AQVLEVFPLLPLVANEPLGIGALSYAGALTIAIVADRDAFPDFEALGAAVSNELRSLT
                     EQPVAAPSRRVATAT"
     gene            complement(23845..24423)
                     /locus_tag="VFV59_04685"
     CDS             complement(23845..24423)
                     /locus_tag="VFV59_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEU5325161.1"
                     /translation="MSRVQVVYASRHGGTKGIAERIADVLRSEGAEVVLSDAADRPAA
                     TGFDAHVIGSGVYIGSWLKEGLEFLERNQSILAQQAVWLFSSGPLPGSSKETDTADPV
                     ENALGPADGPGSGGRKKVEAMSAAIHPRGHWVFQGAYDPNDSPKSMQERLVRLMPAAR
                     NMLPTGDFREWDAIEAWAREVGRSVVAKVPVG"
     gene            24539..25219
                     /locus_tag="VFV59_04690"
     CDS             24539..25219
                     /locus_tag="VFV59_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5325162.1"
                     /translation="MEIAPGIHAVRLLGAFAFLVAEERLTLIDAGLAGSRHRLLRYLA
                     AIGRSVEELDLILCTHRHPDHVGGVRELVAASGAEVRMHPADATALRVSFREAVASRT
                     PGQLVAYFTRGPEEARPIGDGDVLPALGGLHVIHTPGHTAGSVCFWAPRDRVLFTGDT
                     LQVERGRITFASLLFSDDYLAAQASIARLAELDVGTIAFSHYPPWRDDPPGALRSLAR
                     RAASTQGL"
     gene            complement(25224..27713)
                     /locus_tag="VFV59_04695"
     CDS             complement(25224..27713)
                     /locus_tag="VFV59_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5325163.1"
                     /translation="MQTILELLERAVADHGTTVALVIKPSFRTRTWTYADIGDQVPRL
                     ARVLRDAGIDAGDRVLIWSESRPEWCLAFLALLHAEAVPVPVDVRTADEFATKIAAQV
                     RATAVLASRSTAADASRLELPILSLEELVSEARRVPPLPRPGIGPETLAEVVFTSGTT
                     GDPKGVMLTHRNIASNAAALRSVIPLGRETRVLSILPLSHMYGLTPGFLASMVAGTRI
                     VFPTSRQPAVLARTFREQGVTMLLAVPQAVKLLTNAIERGVEASGRRALVDRMHAIGR
                     HLPMALRRLLFRPILGRLGGQLRYIAVGAAPMNPHVARQWEEMGVTCLQGYGATELSP
                     AVSFTRIERNRIGTVGEPIPGVEVRVARDGELLVRGPNVFRGYWERPDATAAVLDDDG
                     WYHTGDHGTLDAEGMLTLHGRSKDIIVMPDGTKVHPNDLEKALIADERVRDAAVVGVE
                     RAGGDVEVHAALIVVPSAGDQGPDIVEAANRRLGGHQQIRAHSIWPDDDFPRTSSLKV
                     RKPDVLRWISDGDTGPRPPAASALAQAAGTAVERLVRQLDGVNLAAVRADARLSSDLG
                     LDSLGRVELLSLIEEELGISIDDGELEPDETVAGLQALVDAGSGSEPPTGIHGWPLHP
                     VASVVRIGLQQALMVPLVALFYRRRVRGLEHLDGVRGPVLFAANHHLHLDNPVILASI
                     PLRWRWRLSIAASAERVFDTPLRRFAVSLLGNGFPLAQSGSVRRSFDLLGARLDRGYS
                     ILIYPEGRLTVGGPLQELMAGTGLVATMADVPVVPVRLSIVRPGRIDRGLNGTALRGE
                     VEVAFGAPLHFGSDVDAAEATERIRDAIERL"
     gene            27899..29062
                     /locus_tag="VFV59_04700"
     CDS             27899..29062
                     /locus_tag="VFV59_04700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5325164.1"
                     /translation="MSQPEIIVVGAGVQGASLAFHLARAGAPPLVIDRPGLDSATGRS
                     SGMVRMHYDLPENAALAWRSLAYFRNWSELVGGHCGFTRTGFLQLVPPELNELLAANV
                     GLQQSLGIATQLMSADDVKALAPEMNTSDVELAAYEPESGYADPTSAAASLLAAARAC
                     GASVRHAQVTAILTDGDRVTGVRTPEGDVRADAVVLAAGAWSGDLARSVAIELPIRAW
                     VHDVAYVVRPAAFGGFPAFIDFGRAMYVRPEGAHLVLVGLEDGNPVLDHPDEVRRGAD
                     GFTDRAVERVAGRLPGLAEGGLHSAHHGVDGITPDQQPIIGPAAPYGPDGLWLDTGFS
                     GTGFKTAPAVGESLARMLLHGADASPELAVFGIDRFHTGRRISPPHPYPPIWH"
     gene            complement(29076..29522)
                     /locus_tag="VFV59_04705"
     CDS             complement(29076..29522)
                     /locus_tag="VFV59_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5325165.1"
                     /translation="MSAAAFGRFYDDGEVIVRQGEPADSLYVVQDGRVEIVAETSTAD
                     VVLRSVGRNEIFGEMAIFGGGVRSATVRSKGRSRVLTLDRDNFMRRITEDPSLAFGMI
                     ESMGNRVRELSHEVVRLRVATGVTRVERATDAMREPGALPRRRRPR"
     gene            complement(29519..30769)
                     /locus_tag="VFV59_04710"
     CDS             complement(29519..30769)
                     /locus_tag="VFV59_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325166.1"
                     /translation="MDLDLEVDIYEPRRFDQPGPIGCNMCGGLMSETLVQNLAADGIF
                     LDTSVVQRGIDSYVLHTDVGSVRIGAPRGEVRIGTVHRGSGPRGAGPSRWGSLDQHLL
                     TSAVRRGAKLIPVRVDDVTRTDGRPTIHARGRTPATYDLAVVATGVNTSLLKALERLG
                     IGYERPRLTRAVIREYRLGEASVQRSLGTSMHVFLLNVPRLEFAALIPKGDYVTMCLL
                     GHEIDGGVADAFAASPEVQGVMPTGFEAAPICQCRPHMNVRGVRRPYGERVVFIGDSG
                     VTRLYKDGLGAAYRTSKAAATTAVFHGISERAFREHFLPLCRDISGDNRTGRVAFRLT
                     RLVRRLRPLRRAMLTIARDEQRTGEGPLSGVLWDLFSGSAPYRDILWRMVAPSLVARW
                     LRVLPAGLRGHSAQAQMTEGPAAS"
     gene            complement(31064..32449)
                     /locus_tag="VFV59_04715"
     CDS             complement(31064..32449)
                     /locus_tag="VFV59_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325167.1"
                     /translation="MTSRTARWLPVAGGMLAVSAVAAVLAAALIWATQGFPALPTSFA
                     RGPINIAIGMAAALSYVGAGWLLVSRVPGNAIGWLLLAIGIVYASMAPTALLVDAASG
                     AYRAAPPLTLMVAWVVSSFSAPVIGVMAIAIGLLFPDGRVPRPYGRLAVILASIGAVL
                     ICIAGAADPAGLVWYPTLPNPYAAPSSFAGALDAAEIAGGMALVAGGAVAVMAMARRY
                     RSGDATVRAQMRWILFAGAILAVAMAPFILVRFILDVGETLGELVLAFVNAATAILPI
                     AAAFAITRYHLFGINLIIGRTLVYVPLMAILGGMYTASIAIFQRIFVAVTGESSDVPL
                     VITIFLVAAAFTPVRKGLEGAVDRWVRGAASAAMPAAAEHPDPAVTRAASELVALRRL
                     EERFAADSRRATGQPTVERRLPIDDESRVPCPAGGRPHFVACLGCRYLDAVLTTPPTV
                     VCSRPAPPAGD"
     gene            complement(32446..33738)
                     /locus_tag="VFV59_04720"
     CDS             complement(32446..33738)
                     /locus_tag="VFV59_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5325168.1"
                     /translation="MPASPERGSRLPGAPIVAVAVMAMALTAAGCSTPQPALTGEVSV
                     LGSWTGDEVEAFRAVVAPFEDRTGVEVTYEETRDLRGVLAERLASGHPPDLAGLEGPA
                     HLAELASAGQLRDLAEILDLGAYRSRVAPTFLELGTADGHLVGVFVRTSLKGMIWYNP
                     SVFTLGSPRTWDELQQMAARASGGETHEWCVGLASEETSGWPGTDLIEQFVLHESGVD
                     AYDAWVAGDLPWTSAEIRRGFELYGQVVAERSVAGGRLQTLETNFRDAGEPLFADPPG
                     CLFLHQGSFMPAFLTSGGDREPGRDFDFFPFPTMASAETDGVLGGGDLLGVLTDSAAA
                     AELMRYLVSDEAQSLWVAQGGTLSVNFGVTDYPDHVSARAAEMLTAAEAFRFDASDRM
                     APGLSTAFREAVLDVTAEPANLDAVLADLEAVAREAPP"
     gene            33910..34356
                     /locus_tag="VFV59_04725"
     CDS             33910..34356
                     /locus_tag="VFV59_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325169.1"
                     /translation="MRLAGFAWVIAAVGTAALTSYLVSRASDPCAAESASCPAVAAEA
                     IRALMWPAWAVLLVPGYLLVSRPTPARARAAALFGLVVGPITAAVIIQLGRSAAAGAG
                     WEARGVAFGALLVVPGLLSAVASRDTDQRASRKRLPARHGHTSPGG"
     gene            complement(34502..>34891)
                     /locus_tag="VFV59_04730"
     CDS             complement(34502..>34891)
                     /locus_tag="VFV59_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325170.1"
                     /translation="GSSQTEYGSSWADAAYLAARDRSIPTIMFSAHAADVREAREAES
                     DRAQAADFAAVLAKPFGLDELLAAVATACHRSQPFNQSPDADRQRTLALARRLRKLEA
                     TDIRTSDRREWATFRPPDSDAIVQLYW"
BASE COUNT         4814 a        12557 c        12543 g         4977 t
ORIGIN      
        1 gagcagggtg ccgtgggcgc cgtcgtggca cgcgccaacg tgacggtgac gcaggctggc
       61 gcacggacgt tgatcgccgg cgagcggctc gaggtgcacc agggcggcgg cgccatgctg
      121 ctgagcggag ggccggtcga ggtcaccatg ggcggcttcg ggacggcgct cgccgccggc
      181 ggcatgcggt tcgagcgtgg cggcgcgatg gtggccattg ccccgtccat cgacgtcggc
      241 gagaactcgg gcgtaggcat cgcgctcacg ccgcgcctga acctcgcgcc gggggctcgg
      301 gtggtgctcg gactgcgcga ggccgcggtc atcggtgccg tggccggcgg tgtgatcggg
      361 acgatcatcg cgctcctgcg ccgccgctga tcggcgcccg accgaacccg acgacgccgg
      421 ctcgagggcc ggcgtcgtgc gtcagtgcag cgccaggagg tcagccctcg gcgaccagcg
      481 agacgaagtc catcgacgag atgaccccga cgggtcgtcc ctgcgcgtcc accgccacca
      541 gccggtgcag ccgatccgca tccatggccc gtgccgcgtc tcgcaccgtg gccgtggtgt
      601 cgatggtgat gggcgggacg ctcatcacct cgccgacccg gatgccgctc tcgcggccac
      661 ggatcagcgc gccgacggtc ggctgggcca ggtagagcag gtcggtgagg ctgatgacgc
      721 cgaccagcag gccatcggcg tcgaccactg gcagccccgt gatgcggtga tgtctcagca
      781 ggtcctccgc ctcctcgatg ctcgcgtcga cggcgacggt gaccggatcg atcgtcatca
      841 ggtcggcgac gcggagctct tccgggcgcg tcgtgcccgg cgcaccggcg atcatcggtc
      901 cgtccgccgt cgcaccaggt cgccgtaggc gatctcggcc gcctcctccc acgcggctcg
      961 gtccacgcgg accgccaccc ggtggctgcc ggcgttgaag ctgatctggg cgtcggatcg
     1021 caccgcctcg tcggcgaaga cctggacgcc ggcgagcgtc gggacgggcg gcacggtgcc
     1081 gacctcccac gccgggcagg ccgcggtcag ctccagctcg gagaggagcc gaacgggctc
     1141 gccgatcagc cgccggacct tggccaggtc gaggacgtcg acggcgtcga ccacgatcaa
     1201 cgccacgcgc ccctcctcgg tgcggacccc gacaaccttg gcgaaggtgc gcggctcgat
     1261 gccctccgcg cgcgcggtgg cgagggcggt gaaggtgagg ggatgcggat ggacctcgaa
     1321 cgtgatcgcg tgggcgtgga gccagttctc caggcgctca tcgatcacgg gggtgctggt
     1381 gaccatcgat cggtcctcca attcgctgcg ttaccgatgc agcgcgggtg ggtgctcccg
     1441 tgccgttacc gacgcggcgc caggctcgcg ctccgtctcg ccccccggtt cgatgctgct
     1501 gtcgtcgaag tcccagcgca gctggtcgtg cagcccggtc actccgtcga ccaggccgat
     1561 gagcttggcc aggatgcgag cggtcgatcg ccggtccacc gtcccggcga tggtgacgat
     1621 gccctcgtca acgtcgatgt cgaaccgcga cggatccagc cacatcgtgc ggcggatgac
     1681 gtcctcggtg acggcgtggc gcacctcgtc gtccaggcgc aggtaggcgc ggacgaggtc
     1741 cgcgcgggtc acgatcccga ccaagcggcc cccggccacc actggcagcc ggttcacgcg
     1801 gcgctcgatc atccgcgccg cggcctcacg gaccggacgg tcggcctcga tggtgagcgc
     1861 gggcgaggtc atggcctcac cggccgtcgt cgccgcccgg cgtcgcagct cgtcggggtc
     1921 gtggtgctcg cccaggatcc actccagcgg cagccggcgg ctgagcgggt gggtcagctg
     1981 cttcgcgagc aggtcgccct cggagatgac gccgacgatc cgtccaccac cttcgtcgac
     2041 gacgggaagc ccgctcacct ggtgctcgct gagcagacgc gccacgtcac gcaggcgcat
     2101 cgacggcgtg gcgcagacca cgccgcgggt catgacgtca ctcaccttca tggctcgatc
     2161 gctccacgct tcaggtcggt ggtcccaccg taggacgtgg ccggcggcgg gcggagagcg
     2221 gttcggaccg attcggcggg gccgaccggc acgccgcccg gccaggctac ggatgaccga
     2281 cgaccaggac cgatgcggtc gcgtggtgca ggacgttgcg cgccacgctc ccgagcagca
     2341 gccgatccag cgcgccgagc ccgcgcgacc cggtgaccac caggtcggcc tcgcgttccc
     2401 gggcgagggc caggacctcg tgcgccggat cgccggcgcg gacgtggccc gtggccggga
     2461 tgccgacggc agtcagacgc tcgaccatcc gatccgccgc ggcggcgaaa cgctggcgca
     2521 gctcctggcg ccggtgcgac agccgctcgt cgccgagcgt gtacaggccg acgatcagct
     2581 cgaaggtcgg ggtgtccggg atcgaaaccg ccacgacgtc ggccgggacg ccggtgaagg
     2641 cgcccagggc ggcgagacgg tccgggatga gctccgcccc ggccgaccca tccgtggcca
     2701 cgaccaggcg cgtgaccgtc ggatgccgtg cgacgagcac ggggcacgcg gcgtgagcgg
     2761 tcacctcgcc ggcaacggag ccgaggagca tcgaccgcag cggcccccgt ccgcgcgaac
     2821 ccatcacgac cagcgccgcg tcgcgcgcgt gcgccgcggc gacgatctcg gtcgccgggc
     2881 gcccggccac gacgtgtcgc tcgacggcca acccgtgctg cagcaatggt gcggcggccg
     2941 cctcgaggtc ctcggccagc tggcctcgcg ttgcctcgtc cacctcgccc atccagtcgg
     3001 ccggcgccat gccgccgctc cagtcgatcg gcgtctggta ggccgccagg aggtcgaccg
     3061 tcgtcgcctc gggccacgac aggccggcca ccaggtcgcg ggcgaccagt gaggacggcg
     3121 aaccgtccag tgccagcagg atgttcatcg ggtgcgctcc gagcggtgtc ctcgccagca
     3181 tcgaccgatc ccgggtcgct gaagagggcc gatcagcccc cgggagcgag gaccttcggc
     3241 gacgcgccgt cgcgcctagt cggcgcggtg caccacgacc cgggggaggc cggcgcgcag
     3301 gagcccgggt ggctccgggt tggcgccggc atcgacgagg gtgttcggcc cgcgctcggc
     3361 atcggcgacg ggcggtcgct cgctggacga ccgctggagg gtgtgcacga ccgcgtcgac
     3421 gacgtacatg cgccgctcac cgtgctccgt gatgtactcg atgcggtcgc cggtgcacgg
     3481 gagctggacg ccgtgctcga ggtccgccga ccagccctcg tcctcgacca cgagggtcca
     3541 cggcagcgcg tcgggagggc cgccgccctg cgccggaccg acggcgtcgc cggtcgagtc
     3601 gcgctcgaga tcgtgcccgc tgcgctcgtc gttcatgccc tccagcaccg gcacgaaccg
     3661 ttccagatcg gtggacgcgc accgtccggg cctgccatga tccgccggca atggagctgc
     3721 acgaggacca ggtggcgcga ggccggcggg agcgctggtg gcgttcgacc ttcgtcatca
     3781 tcgccacggt ctacgtctcg ctgctgctca tcggcctcct gctcgacatc ctcagcggct
     3841 tcgcgcagat cgccttgacc ctcttcatcg cctggctgct ggcgttcgtc ctctcgccgc
     3901 tcgtcagctg gatggtcacg cgattccggt tgccccgcgg cctggcggtc ggcatcgtgt
     3961 tcggcgcggc gctggtgatc ggcgcggcgt tcatcttcta cgctgcggcc tccgtcgccg
     4021 ccaacgtcgg cgacatgacc gccgagttcc cgctcacccg agcgcgcatc gagggcgtgc
     4081 tgcgcgagtg gcaggacgcg atcaccttcg gccgcttcgc gccggacctg gtgcaggtct
     4141 accgcgacgc cgagcgatcg ctggtgggcg tcgccgggcc gatcctcggc gaggtgccgc
     4201 aggtcggcgc ctcggtcctc ggctccctcg cgctggtgat catcctgagc gtgtacatgc
     4261 tggccgacag cgccgggatc ctcgccaagg tcaaccgggt gatcccgagc cggtacgagg
     4321 acgaggtcga gatcctggag cggacgatct cccgtgcctt cggcggcttc ctccgtgccc
     4381 aggtgctgct ggccgcgatc cagacggtgc tcacgatcct cgtcgtgatc ctcgtcggac
     4441 tgccgtacgg gttcctgatc accgccgcat cggcgctggg catgctgatc ccgttcttcg
     4501 gcccgccgct cgcgctcatc ccgccgatcg tggccacggc catcttcgcc agtgactgga
     4561 tcatcccggt cgcaggcggg ctgttgatcg tgcagacggt gctggtgaac tacctccagc
     4621 cgcgcctcat gcaggaggcg ctgggcatgc atccgctgct cgtcctggtc ggcctcctca
     4681 tcggcgcgca ggtcgccggg ctgtgggggg cgctgttcgg gatcccggtg atcgccgtgc
     4741 tgaacgtctt cttcaactac gtcgtgaacc tgcggacgct cgaggagtcg cccgaagaga
     4801 ccgaggaggt cctcgaggag gtgcgggccg aggcgccgga cgccccgccg gaggagctgg
     4861 tggcgatggc cgccgaccgc gtcgaggccg aggaagcgga ggaggaggcc gaggcggccg
     4921 agtcgatgga tgccgcctcg gacgacctgc gtgccgccgc gggcgagctc cgggcggcgg
     4981 cgggggagca gcgtcaagcg gccgggcaga tcggcgagtc ggcggccgag cttcgcgagg
     5041 ccagcgagcg gctggcggag gatcggccct cctaggcggc tccctcctgg cgccggtgca
     5101 ggacgcaggt ccgctgcacc agcgcgtcca gctcggccgg gtcgaagggc ttgtcccgtg
     5161 ccggcgaggt gaacgagagc atccggtcgc cggtcagcat gacgaccggg acgctggcca
     5221 gctgcggatc cgcggcgagg gccgacgaga gctcgaagcc gtccatcacg ggcatccgga
     5281 cgtccgtcag cacgacgcac ggaatcgggc cggtctggag ccgtcgcagc gccgcctcgc
     5341 cgttgggcgc cacctcgacc cgatgtccgg actcggccag cgcgtcgacg tacagggaga
     5401 ggaccatcga gtcgtcgtcg acgacgagga tggagtggct cttgggcgtc atcgtccccc
     5461 ttccgctggg tgctcggtgc ggtccgtcga gagcttcgca tgcacgggac gtgccgtccg
     5521 cttctgacct gcgactggcc gaatcgtccc acgcgacccg gtacccggac gcccctgggt
     5581 cacccccgcc gaccggacca ccgggctact gaccgtgacc gcaccgctcc gtacggtatc
     5641 gctcgggcgc cgagccgccc taccaccgca aggcaccgtc accggagcac cagatgtctg
     5701 ccaccgccat cacctcctcg cgccgcgtcg tcgctcggac gcttggcctc ggaatcagtg
     5761 cgctcctcac cgccggcctg ctggcctgga ccccgaccgc ggtgtccggc tggaaccagg
     5821 gctcggccga ggcgacgctg tggcagctga tgaacggcgc ccgcgtcaac aacggcatcg
     5881 cgccggtgca gtccaacggc acgctggtgg cgctcgcgcg ctggcgcagc gcggacatgc
     5941 tgcagagcaa ctacttcagc cacaccatcc cgagctgcgg ctgcctggtc tacaccttct
     6001 acgaccgcaa cggcctgtcc tacgactggg ctggcgagaa catcggctgg aactcgggcc
     6061 tcgacgacgc ctattcgccc gtccgcgtgc acgaagcctt catggcgtca gccgggcacc
     6121 gcgccaacgt cctggacgcg cgcttcacgc acggcggcgt cggcgccgcc gcggccgacg
     6181 gccagatgtt ccagggctac gtccagaaca cgcgcatgta caccgagctg ttcatgcagg
     6241 cccgatacag cgcaccggca ccggcccccg cgccgcagca gcccgcgccc gcgccgcgcc
     6301 cgccatccgg cggcggcagt ggtggcggcg gcggcggcgg tggcgccacg gcaccgcgca
     6361 cgacgacggc gctccggccg gcggccacgc cacagccgac cccgaagatc gtgagcatgg
     6421 ccgccccacg ccggcccagc tcggccgccg gcatcgacgg cgtcgcgcag gtggtcaacg
     6481 ccaggccgcc gtcagtggcc gcgctcgtga ctcgattcac ggtcgacgag ctggatcgcc
     6541 ctgccaccgg acgctggacc gccgccgcgc cggcgaggga tggcaaggac gcggcctcgg
     6601 ccgcgctgac gcgcgttccg ctccaggtcg cggccgcgcc cgttgacgac ggcgggttcg
     6661 gcggattcct cggcgcgatc atcggcttcc tcttcggctg acctccgacc gcccgcggtg
     6721 cgacggcgca ccgcccgggc gatcggtcga cgccggtaga ctgcccgcga tgcccgtcat
     6781 cctcgaggcg cgtgacctcg tcaagtccta cccggtcggc gacggccacg tcacggccct
     6841 cgacggcgtg tcgctggccg tggaacgcgg cgagttcgtc gcgatcatgg gatcctcggg
     6901 gtcaggaaag tcgaccatgc tccacctgct cggcggcctc gacctgccgt cgtccggcga
     6961 ggtcgtcatc gacggcgtgc gcatctcggc actcgacgag gagcgacgca ccctcacgcg
     7021 acgcgagaag accggcttcg tcttccagtt cttcaacctg gtgcccacgc tgacggtggc
     7081 cgagaacgtc gcgctgccgt tcctgatcgc cggcgagcag ctgtcgcgtc accgggagcg
     7141 cgtggacgag ctgctggcgc tggtgggcct gaccgacaag gcggcgtatc ggcccgacca
     7201 gctctcggcc ggggagcagc agcgggtcgc cctcgctcgc gcgctggcga cggagccggc
     7261 catcctgctg gccgatgagc cgaccggcaa cctcgactac acgaccggca ccgagatcct
     7321 cgacctgctg tgggactcgg cggaccgcct gggccagacg atcgtcctcg tcacccacga
     7381 cgcgcgggcc gccgcgtacg ccgatcgtgt cctggtcgtg cgggacggca ggatccgcga
     7441 cgagatcgcc ctgggccggc gcgccgacca cgaggcgcgc ggcctgatca cgcgcctggc
     7501 cgagctgggg ctgtaggcgg accgatgcgc ctggcgaccc tcgcctggcg cgggctcctc
     7561 gcccgccccc tgcgcacggc gctcaccgtc gccggcgtgg ccttcggcgt cgccgtgctg
     7621 gccgccacga tcgtcatcgg ctcgtcctcg gagcgcgcgc tccaggggtt gagcgccgac
     7681 ctcctggggc gcgccgacgt ccggctgcgg gcattcgagg aggccggctt cacgccccgc
     7741 acgctgcagg ccatccgcgc cgatcccaac gtcgtcgccg ccgccccggt cgcgcagcgg
     7801 cgcctgaccg cgtacaccgc ccccggtgag ggcgagcgcg tgttcagcgt gatggccctc
     7861 ggcatcgagc cggcagccga cgccgaggtc cgcccgccat cggtggtctc cggccgcgcc
     7921 ctcgacccgg acgcggcgga cgaggtcctc gtcccggccg ccttcgccgc gcgcaacggc
     7981 ctggggctcg gaagcgaggt acggctggac ggccggcggg agggcctgcc gcccttgcgc
     8041 atcgtcggcc tgctgcgcga caccggcctg ggcgcggtcg agggcggcga cgtgatcgtc
     8101 ctcgcacggt cgacgctgga cgactcgttc gcggtgccgg cgcccgtgcg ggccgtcgac
     8161 gtcgatctcg gcgagaaccc gacgagcgcg gagatcgacc ggctgaccgc gttgctggac
     8221 gagccctacg tggtggagac gccggaggac gccgcccgcg gcgtggccgg ggcgcagacc
     8281 gcgttcgggc aggtcgccct gctgttcggc ctggtcgcgc tggtcgtcgg cgcgttcctg
     8341 gtcggcaaca cgctcgccat gacggtcagc gagcgcacgc gcgagctcgg tctgctgcgc
     8401 gcggccggca cgacgtcgcg gcaggtcctc ggcatcgtgc tgcgccaggc ggccgccatc
     8461 gggcttgtgg gcagcgccgc cggcgtcctg ctggggctgc tgctggccac gctcctggcc
     8521 ggcctcgtca gctccacccg ggccgtcctg ctgaccgggc tggtcgtgcc gccggtcggg
     8581 atgatcgtcg cgttcctcct gggcctggcg gcgacgctgc tcggcgcggc gttccccgcc
     8641 gcgcgcgcag cacgcctctc gccgctggac gcgctgcgtc cgtcgcgccg tccggagcgc
     8701 ggcctgctgg agcggctccg gccgctgatc ctgctcgagg tggcggcggt gctcatcggc
     8761 ctggtcgtca ccgtcgtcgt cggaggcggc agccccgtgc tccccatcct gctgtccctc
     8821 gccctgctca tcggcggcgc cctggccgcg gccttcgtgc tcgagccgct cggccgcctc
     8881 atcggtcggc cgttcgagtg gttcttcggg gcgcagggcc tgctgggacg cgccagcctc
     8941 acgcgcgacc gggcgcggac cgggctgacg gtcggcgcga tgatgatcgc cctggcggcg
     9001 gtggtcgccc tgggtaccgt cgccgagtcc gcacgcgccg gcacggaacg ctgggtggat
     9061 tcgatcctgc ccggcggcca cgccatccgc accagcctgc cgctcgacgt cgagaccttc
     9121 cgtccgacca tcgaggcgac ggccggcctc cgggtcgcga gcccgctgct cgaagtgccg
     9181 gtcatccggg tcgacggcga cacgcgcgag gaggccatcg tcgtggggat cgacccgaac
     9241 gtgttccagg acaaccgtgc cctcgtcatc cgcgacggcc ggcgtgctgc tgccttctcc
     9301 gccctgcgcg ccggcggggg agtgctcgtc cccgcggccc tggcggaccg cgccggcatc
     9361 gcggccggcg acaccctgct gctcggcgtg ccgggcggcc aggcccatga gatgaccgtg
     9421 gccggcgtgg tggagtacac gctgccggcc cgtacgccgg acggcgccct gctggtcggc
     9481 agcgccgacg ctcgcgatct gttcggcgcg accacggcca gcctgtgggt gctcgtcccg
     9541 cagcccacca tcggcgacgc ggccttcgcc tccgcggtcc gcgagaccgc gctccagctg
     9601 gccgccgagc cgctgacggc cgcggacctc gcgcgtgacc tgtcgcgatc gctggacagc
     9661 ctgaccgggc tgctcgacgt tctcgccctg gtcgccgtcg ccatcgctgc gctgggcatc
     9721 gtcaacacgc tcggcatcgg cgtccaggag cgcattcgcg agatcgccat cctgcgttcg
     9781 cacggcatga ccgtcgggca ggtccaggcc atgatcgtca ccgaggccac catcatggga
     9841 gccgtcgcgg gggtcctcgc cgtggcgacc ggcgtcgtcg tcgcgtccgc gctcgtcaac
     9901 ggtggcgtgg cgacgcagct cgatgccggc gttcggcttc cctgggcgct gctgatcgcc
     9961 gtcgtgctca ccggcgcggg gatcgccgcc ctgtccgggc tctatcctgc gagagccgcg
    10021 gcgaagctgc cgatcgtcgg cagcctgaag cacttcgagt aggaggacgg atggcgacca
    10081 ggttctggga ggcgcgcgcg ggccgcgcgt tcgtcggtcg actcgagacg gggagcgacc
    10141 tggtcgagga gatcgagcga ttctgcctgg agcagagcgt gatggcggcg caggtgaccg
    10201 tcatcggcgc ggttcggcgc gcgcgctacg cctactacga gcaggacgac caccgctacc
    10261 gcgagctgga gaccgatacg caccacgaga tcaccgggtt cgtggggaac atcagcctca
    10321 aggacgaccg ccccttcctg cacgcccacg ccaccttcgc ggatgccgac ggggccaccg
    10381 tcggcgggca cctgctgcgc gggatccagg tcttcgcggc cgaggtgatg atccgcgaga
    10441 tgggcggtgt caccctgacg cgcatgcacg acgaggaaac cggcctctcc ctctggtgac
    10501 cgcgtcggtc gggcacggac cgtgcaagga cgggcggcaa cgcccgttca tcaaggagga
    10561 gccggtgccc gccatcctca tctgggtcct gtggatcgtc gtctcgatcg tcctgttcga
    10621 cgtgctgttc gtcggcgccc gcgtgctcgc cacgcgcgcg tgggaggtcg agcgacccgt
    10681 ccgccgccgc cgccgctagc cccgcccggc gggtccgccg ctaccagcgc ggcccgctga
    10741 acgccggacg ccgatcctcg tgctgcggca cgcgctgcgg cactggaccg gtcgcgacgg
    10801 cgcgctcgat cgcctggagc aggctcgccc gcgtgggggc gtgcaggacc gggacgtcgc
    10861 tcagctcggg gttgttctcc agcgcgtcca ggatcgatcc ggacgaggtg aggacgatcg
    10921 gcttgtcggg gtggacccgg cgaacgtcgg cgatcaggtc cgcgtggccg ggcgctccct
    10981 cccagatgtc gtagaccaag acgtcggcct gctcgacctg ccagcacggc tggccggagg
    11041 ccaccggaca cggatcaccg ccgatcgggc ccgcgcactg gtccacctcg tatcccgcac
    11101 ggcggagctc gtcgacctcg atgtcggcga cgtccacgtc gtgatagacc accaggactc
    11161 gcgtcatctc gttgctccct gccgtctgtt gccagcggcg gtcagcgtcg ccgctggcgg
    11221 tgtcgagggt cggtccgccg atgggggagc gacagggtca aagggcacct tgtgacggga
    11281 caacggggaa ctggccgaga agcgtccgtc tgccccttgc caggcgggca ccgcgggcgc
    11341 aggctgggcc gtcaccggac agcgcgcgag ggcacgggcg agatgcgacg ggtgggcggc
    11401 caggaggtgt cgggcaccgc tggctttcgg atcacggaag agggtcccgc gccgccgtgg
    11461 atcccgcgct tcgaccgcga cccgatcgtc tggctccagg cggtccgtcg ctggcggctc
    11521 ggcccgacct ggatgcgcgt ggtcgccacg ctcgaccccg gagagtggat ctccactgcc
    11581 ggccatcgct ggcgcatgct gcggacggtc cgcggcgtcg agatccgtcc ggacctggtc
    11641 gcgccgttcc ttcacttcgg atcgttcccg atcgtgacgg cactcgggat cggggtggcc
    11701 gtcgaagtgg agctgacgac cgagagcttc cgctgggtcg acgtcttcgc gcccgagcag
    11761 ttcgacttcg ccgagctcgg ccgcgcgatg ctggaggagc gacagctgcc gcatgacaac
    11821 ctgccgctcc tcttcccggc ccgctgagcg ccggccgagg gtagccgtgc ggcgttcccg
    11881 gcgctgaccc cgcggcgtac gatgccgccg tgacggcagc cgagtcctcg cgacgcggca
    11941 cccagcccgc ggtcgtcgcc cgcgggctga ccaggcagta cggccgcgtc cgggccgtgg
    12001 acgggatcga cctcgacctc ccgcccgggc ggatctacgg gctgctcgga cccaacgggt
    12061 cagggaagac gacgctcatt cggctgctga ccgggctggc gcggccgacc tcgggttccg
    12121 ccagcgtcgc cggtgtccag atgccctcgc gcgccaacct ggcgcgcatc ggctacatga
    12181 cccaggccga cggcgtctat ccggagctgt cggtgtggga gaacctgcgc ttcttcgccc
    12241 gcctgtacgg accgatcgag cgctccgcgc tcaccgagtc tctccgtctt gtcgagctcg
    12301 acgcggcggt cgatcgaccg gcggaccgtc tctccggtgg catgcgccgg cgactctcac
    12361 tggcctgtgc cctggtgcac gaccccgagg tcgtgttcct cgacgagccg accgtcggtg
    12421 tggacccggc cctccgggtc tcgttctggg agcacttccg cgcgctcgcg gatgcggggc
    12481 ggacactgct cgtcgccagc cacgtcatgg atgaggcgga gcgctgcgac gtgctcctct
    12541 tcatccgcgc cgggcgggtc gtggccagcg gcagcgccga tgacctgcgc cggcgcgccg
    12601 gcacgcccaa cctggaggag gccttcctcc gctttgccgg cgaggcgggg gtcgggtcgg
    12661 ccgaggagct ggcgccatga acctgcgccg cgtcgccagc ctgacccggc gcatcgtgga
    12721 ccagttccgg cgcgatccgc ggacgctcgc cctgctcttc gtcgcgccca tcttcatcat
    12781 ggccctgctc ggctgggtgc tgcgcggcca ggccaacccg cagccgcgcg tcgcggtcgt
    12841 caacgaggcg ggcacgtcgg ccgagccggt ggtggatgcg atcgaggccg ctctggcaga
    12901 cgagggtgga tccatcgtcg gggtgggttc ccgcgaggcg gacgcgcggc aagccatcga
    12961 cgagcgacgg atcgacgtcg cgctcgtcgt cccggagggg ttcggtgccg gcggagggga
    13021 gctgctcgtc atcacccccg gcctcaaccc gccggcggag ggagccaacg tcctggcggt
    13081 ccagcaggcg atcgcggccg ccgtccgcag cagcgtcccg ccggcgatgg cggggctcgt
    13141 tcctgaggtc cggcacgagg cgttgtacgg accggccgac gccgaccagc tcgacacgct
    13201 ggcgccggtg ttcgtcggct atttcgccta cttcttcgtc ttcatcctca ccggcatcag
    13261 cttcctccgg gagcgggtgg gtggaacgct cgagcgcctg atggcgacgc cgatcacgcg
    13321 cggcgagatc gtgctgggct acagcatcgg cttcggcatc ttcgcgacca tccaggtggc
    13381 cctggtcctc gccttcaccg tgctccgcgt cgacgtgccg gcggtcggac ccgtgcccgc
    13441 ctggtggttc gggctcggcg tgccggtggc gggcagcccg ctgctcgcct acctggtggc
    13501 gctggtgctc ggcctcgggg cggtcagtct cgggatcttc ctgtcgacgt tcgcccgcag
    13561 cgagctgcag atcctgcagt tcatcccgat cgtcatcatt cctcagggcc tgctgggcgg
    13621 aatcttctgg ccgatcgaga ccctgccgag cgtcctcgaa ccggtggccc gcgcgctgcc
    13681 gatcacctac gcggtcgagg ggctgcgcga ggtgttcatc gcgggcgccg gcctcgactc
    13741 gccgacggtg cagctcgacc tcgccgtgct ggccggcatc gcggcgctgt tcgtggtgct
    13801 ggccgccgcc acgattcgtc gcgaggtcgc ctgagcctgc ggttcagccc tcgcgggcag
    13861 cgggccaggt ccccatcacc tggcggagga tcgcgaactc gccggtgtgg tacgcggtgt
    13921 ggtccgccac cacgcggatc tcgcgcccga ccgagtgccg tggggcgtgg gccatcggtg
    13981 ccaggagatc gacgctggcg tcggccacga tcgccagcag cgcctcgcga tcccgcgtga
    14041 agccggcgac ggtggtcgcg aacgcctcgg gtgtcgcggt cgcctcggga tcgggccagt
    14101 agccgatcgg ccagtcgggc gagacgtgcg acgggttgcg gacgtactcg aggatgtccc
    14161 actgcgtgat ccgcacgtgc tccgccagct gccacggtgt gtacgtcacg ttcggcgccc
    14221 gatcgttcac cgcctcgtcc gggaagtcgg cgaccgcctc gaggaagtcc atgtgtgcgt
    14281 cctgcccgcg cagcaggcgc gtcagctgct cgcgcagcgc atcggtgtcg tccatggcgt
    14341 ccacggtaca acggtggcag acgccgttcc gtggaccgca tgtcgcaagc agggcgacgc
    14401 cggccagagt agagtgatat cgcactcggt cgcgtcactt ctccccccgg tggcgcgccg
    14461 agtgcactcg tgtctgcggc cgtcagcggc tgtccgcgcg gcgggatgga gagcgtcagt
    14521 ccgcgtcggc tgcggccatc caggtcaggt ccgagtccac catcgcctcg tcggcgtgcg
    14581 cgatgtgtcg gagctcgcgc cagatgagga ccacgaagat ggccgagccg acgaaggcga
    14641 accagaacgg cgccgtgacg cccatcgact gcgccagcac accgcccagc gcggacccga
    14701 tgaccaggcc gccgaatgtg ccgacggtgt tcacgctccc cacgcgcccc tgcagctcgg
    14761 tcggcaccgc gcgctggcga accgtgatgg acgtggtccc ccacacgaag gcgtgcgcgc
    14821 cgaagacgaa gaagatcacc atcgcgaccc acggcacgct ggcgacggcc agtccgagat
    14881 gggtgagcgt ctcgatgatc aggccgatcc gcatcaggtt gcccagggaa acgcgtcgcg
    14941 tgatccagcc gtacgagaac gttccgacca gtccgccggc tgcggagatc gtggtcagca
    15001 acccgtatcc gatctccccg aggcccaggc gctgggttgc gtagaggacg aggatcgacc
    15061 acgcggcccc gaacgtgatg ttgaacgtga agatcgtcag gaccagcgtc cggaccgccg
    15121 catggtgccg cacccagcgc acgccctcgg cgatgtcgtg gcggatggcc gtctcgcgcc
    15181 gctcggacgc ggccggcgga agcgcgattc ggctgaccag cgcggccccc gccaccacga
    15241 gcagcgcctg gccgacgaag ggcagcgcca tgcccagcgc gaacagcgcc gcgcccaggg
    15301 gtgggccggc gagctgattg accgtgacga accccgccat cagccgcgag ttggcggtcg
    15361 ccaggtcgtc gcgccggacg agcatgggga ggagcgtgtg agtggtgttg tcggcgaata
    15421 cctcggcggt tccgaggacg aacagcgcgc cgagcacgac ggcgacggag atcgtgtcgg
    15481 ttaggaccga cgcggcgatc acggccagga cggcgccccg caccaggttg acggtgacga
    15541 cgatgagccg gcggtcgagc cgatccgaca gcgctccggc gtacagcccg aagagcagcg
    15601 gcggcagcca ctgcaccagg gcggccatgg cgaccaggaa ggcatcctcg gtctgcgagg
    15661 ccacgagcag cgggccggcc gcgatcgcga tgccgtcgct caggttcgtc gtccacgacg
    15721 acgcgagcag ccagcgaaag ccgggcccca ggcgggccgg cgccgcaccc tcgaggagcc
    15781 acctcagccc gcgcaccgcg gcagtctagc gaccgatacg cgcgcccgcg cccgggcgcg
    15841 cggcgtgtcg gtaccagggg cgcatggccc attcgggtgg tgcgcgccgc gcgcgcatcg
    15901 gctcagactg cgacgtgatg accgatctcg agctcgagct tgcggccctc cgccgacgcc
    15961 gccgatcgcc cggctttgca gcctggctgc ggcagttcgc gccgttcgac cggcgcgcca
    16021 tccgcgaccc ggatgagcgc gacgcctact gggtcacggt ccagcagcgg ctggtggaga
    16081 gcgacgcgca gtacctcatg aactctcgcg cgacggagcc gcccgtccgc accgatctgc
    16141 cgctcctgat cgggggctga cgccgcgagg ccgccggccg cgtgctgcgc ggtcccgacg
    16201 gcctcatcgg gcagcgcgac gtcagaggct gcctcaggcg ccgattccga agacgttgat
    16261 gacgcccagc agatcgagca cgaacacgat caggccgatc aggatcagga ggctgagcac
    16321 gtcgtagagc gggctcgcgg tgccgtagta gccggcgcgc ggccccaccg aggccagcag
    16381 cagcacgacg agcacgatga cgatgatcca ttccatgggg cgtcctcctc gttgcgatca
    16441 cgccaccgac cctcggccgc tgacgacgac ccgagcacct tagcccgcat cgcgccgtgg
    16501 cggaacccgt tcttcgccga tcgccggggc cagcggatcg gcgcgtcggc tcaggagcag
    16561 ggagcggaat cgccgggcta gacccccgtg gcgtcttctc aggtggccga gcttggcgtc
    16621 cagacggccg agagcgcgac ccacagcagc aggagatcga gcgcgagcgg cgcgaccgcc
    16681 cacggtccga ggtggaggag gaagagcgcg agggagccgc tggctcccac gatcaccagg
    16741 gcgcgccaca agtcggccgc gacccagatc ccgaatgtcg ccaggaaggc gccgatgaag
    16801 gcgagcacgg tcagcccggc gacggccagc atcagcacca gcgggaagcc gtcgatccgg
    16861 ccgaaggtcc aggtcgcctg cgcgccctcg cgcccgaacg ggaagacgag ccaccatggt
    16921 gacggcgccg gcgccgggat gatgtagctc atgacgaagt tggcggccag aaagatcgcg
    16981 gccgcggcga cgagtcgctc gagcatggtc gtgtacctct tcgaccccga cgggcgtcag
    17041 gcgtggatcg cgtccagcgt cacgcgtcgg gcgtggatgc ggcagatcac acgcgtctct
    17101 ctcgcgcgct gctccgccgg gtagacccac ccgtagaacc gtggatgggt cgtgtacttc
    17161 ctggtcagcg catcgaggtg ctcctccgcg cctgcgaaga cgagctcggc gtcgccgcgc
    17221 atctggatga agcggccggt gttcgtcgga tccacgacga ggaggctgac cttcgggttc
    17281 gacaccaggt tgcggccctt gcggcgctcg agcgtcgtat tcaccctggc gcacgagccg
    17341 tcatggtcga cccagaccag gctggactgc ggctggccct cggcgcccat cgtcgtcagc
    17401 acgccgcaga tcggccgcgt gagcagatcc aggtgcgagg cggggagcga caccacgtcc
    17461 cggaccgcga caccggtcat cggctgcctc cgcctgcggt ggcgtcgatg gccacgacgt
    17521 gcgtcggccg gatgcggcac agcaccggcg tctcggtgga tgcgaactcg gcggggatcg
    17581 cgtcgccgaa gtaccggatg ggtcggccgg cgtagcggga cgccagcgcg tccaggtgct
    17641 cgagcgcgcc cacctcggtc atttcgacca ccgtgccgcg cacctccagg tagcgcagcg
    17701 gctccgcggg gtcgtagcac aggagcgtcg ctcgtgggtc gcgccgcagg ttccgctcct
    17761 tggcgaagcc ccgcatcgtg ttgacgcgga cacactcccc atcgaagtca caccacacca
    17821 cggacgtctg agggtagccg tcggcgccga tggtcgacag cgccgcgacc ggtggcgcgt
    17881 cgacgaggtc gaggtgcgac ggcgggatcg tggtgcaccg cgcgccggtg acgaggccgg
    17941 tgaccctcat gggaccgccg ctcagacggc cacgtcgctc ggcgtccagc ccaaccacag
    18001 cgccgccgcg agcacgacga cgtccacggc gagcgcgccg agcgtgctga acaccgggaa
    18061 ggccaccggg aagaggatga tcccggcgat cgacaccagc gacgagccga ccgccagcgg
    18121 aacccaccag tccgccggga gccacccgag gacgacggca ccgacggcga tgaagccgac
    18181 gagcgccagc gtccacagga ccgtgccgac gacctgcgtc aggctcgggc cgaccccgct
    18241 gaggatccag gagtcaccgt gccaagcggg attgatcgtc gcgatattga acatgccgag
    18301 ggggcccatg ctgtggccga tgccgtgggc catcaggacc agcgcgatca ggatcttgag
    18361 catcagggtg cacctctggc ggccgcggca agcggccgat acgctggttc gacgggctgg
    18421 agcgccgcgt cgtcgaggaa ggcagtgacc gcctcgaggg tctcggtcgg ccgctcggcc
    18481 tgcgggttgt gccccgcgcg ctggatgatc cgcagtcgcg cgtccgggat gccggccgcg
    18541 agcatcgact ggctctcggg cgggaagagg aagtcgtcgt gcccggccat caccagggtc
    18601 ggcgcgcgga tctcacccag ccggtccatc acggtccagc cgcgcatcat ccgtccgccg
    18661 aacatgaggg cctcgggccg cgtctgcatg cgccagccgc ccgcggcaag ctcgcgggcc
    18721 agcttcagca ggctgaagcg cgagtcgtag gcgggaagca gccggaacga ggctcgaacg
    18781 aagtccttgg gcgcgacgcg acccgagtag aagcgacgcg ccaccgcgac cgtcttgcgg
    18841 tcgtacccac gctccgcgag gacatcggcg gcgcgctcct gcgaccagcg ggcgtcgccg
    18901 gcggtgtcga gcaggaccag gtgtgagacg cgctcgggat agcgcagcac gtattcgagc
    18961 gccacgtggc cgccgaacga atggcccagc acgtcccagc gctcgaatcc caggtgctcg
    19021 cgcagcgcat cggcgtcggc ggtgaggttg tcgaaggtca tcgacgtgat cggcgccccg
    19081 atcgaccggc cgttgcagcg gtggtcatac aggacgaccg tgtggcggtc ggccagcgca
    19141 cggaacgggg tcagggagat gtggtcgaga cccggtccac cgtgcatcag gacgagcggc
    19201 ggccccttcc cgacgacctc cacgtagagc ggtacgccgc ggatcgtgat ggtcggcatg
    19261 gtcgtggtcc ctccgccttc ccgtcgtgtg ccgggactcg acccatgctt cggggcgcgc
    19321 gggcccccga ccagagccgt tcgaccccaa tccggcccgt ggtccgactt cggcccgttc
    19381 ggccctagcc gcgcggaggc gtgtggccgg accatcgggc aggccactcc tcccgaggcg
    19441 cgtgatcgca tgaacgctgt tgccacccgc cgtccgctgg tgccgatgca ccgagaggtc
    19501 gcgccggcgg tcgcggcaat tgcccatcgc catcccgccg tcggcctggc ggtgggcgtg
    19561 gtgtcggacg gcggtctcgc atgcttcgcg gggcacggcc tcgcggacat cgcgagcggg
    19621 acgccggtca cgaaggacac cgtcttccgc atcggatcca tcaccaagac gctgacgggc
    19681 gtcgcggtca tgcagctgtg ggagcagggc ctgctggacc tcgatgcgcc ggtgaatcgg
    19741 tacctgcgtg ccttcccgct ggtcgccgac cgcgaggatt ggcgccacgt cacggttcgg
    19801 cacctgctca cgcacaccgc cggcgtcccc gacgtgcgaa gcgtgcgcga cctgctgcgc
    19861 ggcggcatga cgcccgcgga cggccgacca cccgtgctca gcgtgccttt cggcgaggcg
    19921 ctgccatcgc tcgcggacca ctacgcggac gggcttcgcg tcgtcgccga gcccggaacg
    19981 gcgtgggcct acagcaaccc gagcttcgcc gcgctgggcc aggtcgtcga ggacgtcacg
    20041 ggacagccct tggaccgata cctgcgccag cacgtcttcg caccgctcgg gatggagcat
    20101 tccgacctcg ttcgatccga ccgcgtcgcg tccaggttgg ccaccgggta cgccatgggc
    20161 cctcacggcg cgagtccggt gcaggaccgt gactggatcg gggtcggggc aggcggggcc
    20221 tatgccacgt ccggcgacct tgcgctgtac ctggctgcgc tgctcggcgg cggagcgaac
    20281 gagcacggtc gtgtgctccg cgcgtcgacg ctggccacga tgctggagcc gcaattccag
    20341 cccgacccgc gcgtgccggg catggggctg agcttcttcc gcggcgacct cggcggccac
    20401 cgggtcgtgt accacgacgg catccttccc ggcttcaaca cgtccctcgt ggcggcgccc
    20461 gacgacggca tcggcgtgtt cggcatgacc aacgggtccg ccggcgcctt tgcctggctg
    20521 caggtcgagc tcgacgccct gacgcggcac ctgctgggca tcggcgacga ggaccgccac
    20581 gcgccgcacc acccggagct gtggccggcg ttctgcggtc ggtacgtgct gccaccgggc
    20641 gttgccgacg tgcgtgagcg cctgatgctc ggcggcggca tcgaggtgct cgtccgaggc
    20701 ggtcgcctcc tggcccgggt gctcacgccg atcccgatcc tgttccgtgg cttccccctc
    20761 gaacccgccg accgcgaccc cgacgtgttc cgcgtggacc tgtcgagcct cgggatggcg
    20821 ccggtccggg tcgtcttcgg gactcgggcg ggcggacggg cgatggcgct ccacaccgac
    20881 gtcggaggcc agccgtggtc gctgcgccgg gcccccgccg cgcccgggtg gcgtgggccg
    20941 gcggtgcgcg cgggcctcgc ggccgcggcg gccaccgccg tgctgtggag acgaaggagg
    21001 gtgtgatggt ggcggtgtcg acagcgacgg agctgtggcg gctgggggcg gtcgagctgg
    21061 cgacgctcat cgcctcccgg caggcgtcct gtgtggaggt cgtcgacgcg cacctgcgcc
    21121 gcatccaggc ggccaacacc gagatcaacg ccgtcgtcat cgtcctggcg gaggaggcgc
    21181 tggaggcggc ggcggccgcg gaccgtgcaa ttgcgccggg cgcggcactc cagccattgc
    21241 acggtgtgcc gttcaccgtg aagcagaaca tcgacgtcgc cggcacgccg accacgcagg
    21301 gctaccgagc gctgtcgaac gcgtatccgg cgagggacgc accggcggtc gagcggctga
    21361 aggcggccgg cgcgatcgtc ctcggccgca cgaacctccc caccggtgcc atgcgctggc
    21421 attgcgagag cgagctgtgg ggagtgactc tgaacccatg ggaccgatcg cggacgcccg
    21481 gcgcatcgag tgccggcgag gccgccgcga tcgcgaccgg catgagccca ctgggcctcg
    21541 gcaacgacgg cctcggctcg ctccgccacc ctgcgcagtg ctgcggcgtc accgctctca
    21601 agccgacgct cggacgcgtg ccgcaggaca gcacggtggc atccggcgac gccgggccga
    21661 tcggcatcca gctcaccggc gtcacgggcc cgctcgcgcg gcgcgtggca gacctccggc
    21721 aggcgttcca cgtcattgcc gggcccacgt ggcgagaccc atggagcgtt cccgtggggt
    21781 tcggcggtcc cgacctcgcg ccaccgatcc acgtcgctgt cgtcctcgac cccggtggac
    21841 tggggacagc ggcacccgtg caggacggcg tgagggcggc ggcaacggca ctggccgatg
    21901 ccggctacgc ggtcgaggag cgggagccgg acggcatcga cgccgcggcg acggcgctcg
    21961 tcgtcatgga cagcaccccc gggattcggg cggtatggga gcggatggcg gccaccgctc
    22021 ccgatccggt tcggcggttt gcgaccgcct tcttcgcctc ggccggcgag cctgacggcc
    22081 tggccgtcga ggagagcttc gtcgttcgcc acgccgtgcg gcgggcatgg ggtcagttcc
    22141 aggaatcgca tccgctgatc gtggccccga tcgcgacgga gatgccgtcg caggccggca
    22201 ccgacctgga cgaggggcac gttgcggccg acatccgcgc gatgcggatg gccacggccg
    22261 tcaacgcgct cggcttgccg gccgtcgccg tgccggtcgg ggtcgccgat gggctgccgt
    22321 tggcggtgca ggtcatcggg ccgccgtacc gcgaggacct ggccctcgac gcggcgcagg
    22381 ccatcgagga ccggctgggc gtcctcaccc cgattgatcc gcggtaggcc cgctgccatg
    22441 ggaccgatcg aacggctgag cgtcctcgac cagctcatgc tgtgggccag tcgccggtgg
    22501 ccgcagcaca tcggcgccct gctcatcctc gagggcgagc cgctgctgga cgcggcgggg
    22561 gagctgcgca tccaggagct gcgggagcgc gtggcgtcgc ggctgcacct cgtgccgcgc
    22621 ttccgccagc tcatcctcag acctcgtcgc gggctcggtg gcccgctctg ggtcgatgcg
    22681 ccggcctttg atgtcgcccg ccacgtgcgc cagatgcggc tggaaccgcc ggcgagcgag
    22741 gcggagctgg tggccgccat cgagggtctg ctgctggcgc cgatggatcc cgggcaaccg
    22801 ctgtgggaca tgcggttcgt cgtcggactg ccggagcgtc gcatcgccgt gctgctgcgc
    22861 atccaccatt ccatcgccga cggcatggcc gctatgagaa cggtcacacg cttcctggat
    22921 tcgtctccgg aagcaccgac ccaggcggcg ccgccctggc ggccggacgc gccgccgtcg
    22981 tcacgcgcgc tgctcggcga caacgtccgg cggcggctgc ggggcatcgg ctcgtcgctc
    23041 gccccgctca cccggccgcg ggcaacgctg cgcaccgtcc gggctgcgtg gccggccatc
    23101 cgcgaaatcc tggccgagga gccggcgacg gccacgagcc tcgaccgcat ggtcggtgag
    23161 cggcgcagga tcgcgctgat ccggtccgac ctgtccaccg ttcgcgccat cgggcgccgt
    23221 cacgacgcga cggtgaacga cgttctcctg tcgatcacga ccgccgggat gcgagaggtg
    23281 ctcctggccc gcggcgaggc ggtcgacggg acgacgattc gcatctacgt cccggtgtcg
    23341 ctgcggcgcc atctgcacgg ccgccaggag ggcaacctga tcgcgcagat ggccgtcccg
    23401 ctccgcatgg gcctggcgga ttccgaacgc cggctggacg ccatcgctgc cgagacgcga
    23461 cagcgcaagc tgcgggcacg gacgtcgctc tcgaacctga tggtcggcgg cacgatcggc
    23521 cgacggctgc tgctcttcgc cgtcatgcgc cagcgcgtca acgcgaccag tgccagcatc
    23581 ccggggccga agcagccgct ctacctcgcc ggcgcccagg tcctcgaggt cttcccgctg
    23641 ctgccgctcg tcgccaacga gccgctgggc atcggcgcgc tgtcctacgc gggcgcgctg
    23701 acgatcgcga tcgtcgccga ccgcgacgcg ttcccggact tcgaggcgct cggcgccgcg
    23761 gtctcgaacg agcttcgttc gttgacggaa cagccggtcg ccgcgccgag ccgccgcgtc
    23821 gcgaccgcga cctgatcgcg gccgtcagcc gacgggcacc ttcgccacca cgctgcggcc
    23881 gacctcgcgc gcccacgcct cgatcgcgtc ccactcccgg aagtcgccgg tcggcagcat
    23941 gttcctggcc gccggcatca gccgcaccag ccgctcctgc atcgacttgg gtgagtcgtt
    24001 cggatcgtag gcgccctgga acacccaatg cccccgggga tggatggccg cgctcatggc
    24061 ctcgaccttc ttgcggccgc cgctcccggg cccgtccgcc ggcccgaggg cgttctcgac
    24121 cgggtcggcc gtatcggtct ccttcgagga gcctggcagg gggccgctgc tgaacagcca
    24181 caccgcctgc tgcgcgagga tggactggtt ccgctcgagg aactccagac cctccttcag
    24241 ccagcttccg atgtagacgc cgctgccgat gacgtgcgcg tcgaagccgg tggcggccgg
    24301 acgatcggcg gcatcgctga gcacgacctc ggcgccctcg gagcgcagca cgtcggcgat
    24361 gcgctcggcg atccccttcg tcccgccgtg gcggctcgcg tacacaacct ggactctcga
    24421 catcgctggg tctccttctt cgatgcgccg agcgtgcgtg gtgagctgca cgttcagcaa
    24481 gtgccggagc gccccttccc ggcgatgagg ccgtcgtcgc ggtccgtatc atcggatcat
    24541 ggagatcgcg cccggcatcc acgccgtgcg gcttctcggc gcgttcgcgt tccttgtcgc
    24601 ggaggagcgc ctcacgctca tcgacgccgg cctcgcggga tcgcggcatc ggctgctcag
    24661 gtacctggcc gccatcgggc gctcggtgga ggagctcgac ctgatcctgt gcacccaccg
    24721 ccatcccgat cacgtgggcg gcgtccggga gctcgtcgcc gcgtccggcg cggaggtgcg
    24781 gatgcatccc gcggacgcca ccgcgctgag agtctcgttc cgtgaggccg tcgcgagccg
    24841 gactccgggt cagctcgtcg cctacttcac gcgtggcccg gaggaggcga ggcccatcgg
    24901 cgacggcgat gtgcttcccg ccctcggcgg cttgcacgtc atccacacgc ccggccatac
    24961 cgccgggagc gtctgcttct gggcgccgcg cgaccgggtg ctcttcacgg gcgatacgct
    25021 gcaggtcgag cgtgggcgaa tcaccttcgc cagtctgctc ttcagcgatg actacctcgc
    25081 ggcgcaggcc tcgatcgcgc gcctggccga actcgacgtc ggcaccatcg ccttcagcca
    25141 ctacccgccg tggcgggacg acccacccgg cgccctgcgg tcgctggcgc gccgtgcggc
    25201 gagcacccag ggactctgac cgctcagagg cgctcgatgg cgtcgcggat ccgttcggtt
    25261 gcctcggcgg cgtcgacgtc ggagccgaag tgcaggggcg cgccgaaggc gacctccacc
    25321 tccccacgca gcgcggttcc gttcaggcct cgatcgatcc tgccggggcg gacgatgctg
    25381 agtcgcaccg ggacgacggg gacatcggcc atggtcgcga ccaatcccgt gccggccatc
    25441 agctcctgga gcggcccgcc gacggtgagc cgaccctcgg gatagatcag gatgctgtaa
    25501 ccgcgatcga gcctggcgcc cagcaggtcg aagctgcgcc gcaccgatcc gctctgcgcg
    25561 agcgggaagc cgttcccgag cagcgacacc gcgaagcggc ggagcggggt gtcgaagacc
    25621 cgctccgcgg aagccgcgat cgagaggcgc catcgccagc gaagggggat cgacgcgagg
    25681 atgaccgggt tgtcgaggtg aaggtgatgg ttggcggcga agagcaccgg tccacggacc
    25741 ccgtcgaggt gttccaggcc acggacccgc cgccggtaga acagcgcgac caacgggacc
    25801 atgagcgcct gctgcagacc gatgcggacg acgctcgcca ccgggtggag cggccagccg
    25861 tggatgcccg tgggcggctc gctgccggac ccggcgtcga cgagcgcctg gaggccggcg
    25921 acggtctcat cgggctccag ctcgccgtca tcgatcgaga tcccgagctc ctcctcgatc
    25981 aggctgagca gctccacgcg gcccagcgag tccaggccga ggtcggaaga gaggcgcgca
    26041 tcggcccgaa ctgcggcgag gttcacaccg tcgagctggc gcaccaggcg ttcgaccgcg
    26101 gtgcccgctg cctgggcgag ggctgacgcc gccggcggcc tgggtccggt gtcgccgtcg
    26161 ctgatccagc gcagcacgtc cggcttgcga accttcaggc tgctcgtgcg cgggaagtcg
    26221 tcatccggcc agatcgagtg cgcgcggatc tgctggtgcc cgccgagcct gcggttcgcc
    26281 gcctcgacga tgtcgggccc ctggtccccg gcgctgggga ccacgatgag cgcggcatgg
    26341 acctcgacgt cgccgcctgc gcgctcgacg ccgacgaccg ccgcgtcgcg cacccgttca
    26401 tcggcgatca gcgccttctc gaggtcgttc ggatggacct tggtgccgtc cggcatgacg
    26461 atgatgtcct tcgagcggcc gtgcagggtc agcatcccct ccgcgtcgag cgtcccgtgg
    26521 tccccggtgt ggtaccagcc gtcgtcgtcc aggacggcgg cggtggcatc cggccgctcc
    26581 cagtagccgc ggaagacgtt cgggccgcgg accaggagct ccccgtcgcg cgcgacccgc
    26641 acctcgaccc cggggatcgg ttcgccgacc gtcccgatcc ggttgcgctc gatgcgtgtg
    26701 aacgagacgg ccggtgagag ctcggtcgcg ccgtacccct ggaggcacgt gacgcccatc
    26761 tcctcccact gccgggcgac gtgcggattc atgggtgcgg cgcccacggc gatgtaccgc
    26821 agctgaccgc ccagcctgcc gaggatcggc cggaagagaa gccgccgcag ggccatcggc
    26881 aggtggcgcc cgatggcgtg catgcggtcc accagggccc gccgcccgct cgcttcgacc
    26941 cctcgttcga tggcgttggt gagcagcttc acggcctggg ggacggcgag gagcatcgtg
    27001 acgccctgct cccggaacgt gcgcgccagc acggccggtt ggcggctggt cgggaacacg
    27061 atccgggtcc cggccaccat cgacgcgagg aacccgggcg tgaggccgta catgtgcgag
    27121 agcggcagga tcgacaggac gcgggtctcg cgacccagcg ggatgacgct ccgaagcgca
    27181 gccgcgttcg acgcgatgtt gcgatgcgtg agcatcacgc ccttcgggtc cccagtggtg
    27241 ccggacgtga agacgacctc ggccagcgtc tcggggccga ttccggggcg ggggagcggt
    27301 ggcacccggc gagcctccga aacgagctcc tcgagcgaga ggatcggcag ctcgaggcgg
    27361 ctcgcgtcgg ccgccgtcga gcgcgacgcg agaacggccg tcgccctcac ctgagcggcg
    27421 atcttcgtcg cgaactcgtc cgcggtccga acgtcgaccg gcaccggcac ggcctcggca
    27481 tggagtagcg ccaggaacgc caggcaccac tccggccggc tctccgacca gatcagcacc
    27541 cgatccccgg catcgatgcc ggcatcgcgc agcacccgcg ccagcctcgg gacctggtca
    27601 ccgatgtcgg cgtaggtcca ggtccgtgtc cggaagctgg gcttgatgac gagcgcgacc
    27661 gtcgtcccgt ggtccgccac ggcacgctcg agcagctcga ggatggtctg catgccaatc
    27721 gacccccgct cgttcgggcc accgtcctcc gcgtccgtcc ttcgtcgcaa gggtcattgg
    27781 gaccgattcg cgctggagcg gttgtgcgtc gacgcgccac ctccgcgccg tcatgccccg
    27841 cgccggaggg ggcatccggc cctgccgcgt ccggctgcgc agcacgaaca atccgcggat
    27901 gagccagccc gagatcatcg tcgtcggcgc cggagtgcag ggcgccagcc tggcgttcca
    27961 cctcgcccga gccggagcgc cacccctcgt catcgaccgg cccggtctcg acagcgccac
    28021 cggccggtcg agcggcatgg tccgcatgca ctacgacctg cccgagaacg cggcgctggc
    28081 gtggcggtcg ctggcgtact tccggaactg gtcggagctc gtcgggggcc actgcggctt
    28141 cacgcgaacc ggcttcctcc agctcgtgcc acctgagctc aacgagctcc tggccgccaa
    28201 cgtcgggctc cagcagtcgc tgggcatcgc cacccaactc atgtcggccg acgacgtgaa
    28261 ggccctcgcc ccggagatga acacgagtga cgtcgagctg gccgcgtacg agccggagtc
    28321 gggctacgcc gacccgacct cggcggccgc gtcgctgctg gctgccgcca gggcatgtgg
    28381 cgcatcggtc cggcatgcgc aggtcacggc aatcctcacc gacggcgacc gtgtgaccgg
    28441 ggttcgcaca ccggaaggcg acgttcgagc cgatgcggtc gtgctcgccg ccggcgcgtg
    28501 gtcgggcgac cttgcgcgca gcgtcgccat cgagctgccg attcgcgcct gggtgcacga
    28561 cgtggcctac gtcgtcaggc ccgcggcgtt cggcgggttc ccggcgttca tcgacttcgg
    28621 ccgcgccatg tacgtgcggc ccgagggggc gcacctggtg ctggtcggtc tcgaggacgg
    28681 caacccggtg ctggaccatc cggatgaggt gcggcgcggc gcggacggct tcacggatcg
    28741 ggcggtcgag cgcgttgcgg gacgtctgcc gggcctcgcc gagggcgggc tgcacagtgc
    28801 gcaccacggc gtcgacggca tcacgcccga tcagcagccg atcatcggtc cggcggctcc
    28861 gtacgggccc gatgggctgt ggctggacac cgggttcagt ggcaccgggt tcaagacggc
    28921 gcccgccgtg ggcgagtcgc tggcccgcat gctcctgcac ggagccgatg cgtcaccgga
    28981 gctcgccgtc ttcggcatcg accggttcca cacgggccgc cgcatctctc caccgcatcc
    29041 gtacccgccg atctggcact gagcgacccc ggcggtcatc gcgggcggcg gcgtcgtggc
    29101 agcgctcctg gttcccgcat ggcgtccgtc gcccgctcca cgcgggtgac accggtcgcg
    29161 acgcgaagcc tcacgacctc gtggctcagc tcccgcaccc ggttccccat gctctcgatc
    29221 atgccgaacg cgagtgacgg gtcctccgtg atccggcgca tgaagttgtc ccggtcgagg
    29281 gtcagcactc gtgatcggcc tttcgaccgg acggttgcag atcggacgcc tccgccgaag
    29341 atcgccatct cgccgaagat ctcgttccgg ccaaccgacc gcagcaccac gtccgccgtg
    29401 ctcgtctccg cgacgatctc gacgcgaccg tcctggacca cgtagaggga atcggcgggc
    29461 tcaccctggc gaacgatgac ctcgccgtca tcgtagaagc gtccgaaggc cgcggcgctc
    29521 atgacgcagc cggcccttcc gtcatctgcg cttgggcgga atggccacgg agcccggccg
    29581 gaaggacgcg caaccaccgc gccacgagcg acggggcgac cattcgccac aggatgtcgc
    29641 ggtagggagc gctgcccgag aacaggtccc acaggacgcc gctcagcgga ccctcgccgg
    29701 tgcgctgctc gtcccgcgca atggtcaaca tcgcgcgacg cagcggacgg agacgtcgaa
    29761 ccagccgagt caggcggaat gcgacgcggc ccgttcgatt gtcaccgctg atgtctcggc
    29821 agagcggcaa gaagtgctcc cggaaggcgc gttccgagat gccgtggaag acggccgtgg
    29881 tcgctgccgc cttcgaggtc cggtacgcgg cgcccaggcc gtccttgtag agccgcgtca
    29941 cgccactgtc gccgatgaac accacgcgct ccccgtacgg ccgacgcacg ccgcgcacgt
    30001 tcatgtgggg ccggcattgg cagatcggcg ccgcctcgaa cccggtgggc atcacgccct
    30061 ggacctcggg cgacgccgcg aacgcatcgg ccacgccgcc gtcgatctcg tggccgagca
    30121 ggcacatggt gacgtagtcg cccttcggga tgagcgccgc gaactcgagg cgagggacgt
    30181 tcaggaggaa gacgtgcatg gacgtgccga gcgatcgctg gacggatgcc tccccgagcc
    30241 ggtactcgcg aatcacggcc ctcgtcagcc gcggccgctc gtagccgatc cccagccgct
    30301 cgagcgcctt tagcagcgac gtgttcactc ccgttgcgac caccgcaagg tcataggtcg
    30361 ccggcgtgcg accgcgcgcg tggatcgtcg gtcgaccgtc tgtccgcgtc acgtcgtcca
    30421 cccgcacggg gatgagcttc gctccgcgcc ggacggcgct cgtcaggaga tgctggtcga
    30481 gcgatcccca ccgggatgga cctgcaccgc gcggcccgga tccccgatgg acggtaccga
    30541 tgcgaacctc gccccgagga gcgccgatgc gaacgctccc gacgtcggtg tggagcacgt
    30601 aggagtcgat gccgcgctgt acgacgctgg tatccaggaa gatgccatcg gcggccaggt
    30661 tctggaccag cgtctccgac atcaggccgc cgcacatgtt gcagccgatc ggtccgggct
    30721 ggtcgaaccg acgaggctcg tagatgtcga cctcgagatc gagatccacc cgagcggcca
    30781 ttgcgagcag ggcgtgagcg aagagcgaac ctgcggggcc accgccgatg acggccaccg
    30841 tcgagcccga ggagagaacc gcggtccctc ttctgctgcc ggtgcgtctg gcgctgcggc
    30901 cttccacttg cgacgctcca tggagacgat gcgggttcac gatgcccccg agacgggttg
    30961 ccggatacgg ccatccgtcg cggcctgggc gcgacatgtg ccggagacgt ggcagccacg
    31021 cggatgcgtc gtgcacatcg acgtcgcgca cgtcgacgcc ccgtcagtcc ccagcaggcg
    31081 gtgcggggcg cgagcagacg acggtcggcg gcgtcgtcag gacggcgtcg aggtagcggc
    31141 agccgagaca cgccacgaag tgcggcctgc cgccggccgg acagggcacg cggctctcgt
    31201 cgtcgatcgg aagccgccgc tcgacggtcg gctgtccggt ggcacgccgg gagtcggcgg
    31261 cgaaacgttc ctcgagcctg cgcagcgcga ccagctcgga ggcggcgcgt gtgacggcgg
    31321 gatccgggtg ctccgctgcc gccggcatcg cggcggatgc tgcaccgcgc acccagcgat
    31381 cgacggcccc ttcgagaccc ttgcggacgg gtgtgaaggc ggcggcgacc aggaagatcg
    31441 tgatcacgag tggcacgtcg gaggactcac cggtcaccgc gacgaagatg cgctggaaga
    31501 tcgcgatcga ggcggtgtac atgccgccca ggatggccat gagcgggacg tacacgaggg
    31561 tgcggccgat gatgaggttg atcccgaaga ggtggtagcg ggtgatggcg aaggccgcgg
    31621 cgatcggaag gatggccgtg gccgcgttca cgaaggccag caccagctca ccgagcgtct
    31681 cgccgacgtc gaggatgaag cgcacgagga tgaacggcgc catggcgacg gcgaggatcg
    31741 ctccggcgaa gaggatccac cgcatctgcg ctcggacggt cgcgtcgccg ctccgatacc
    31801 gccgcgccat ggccatgacg gcgacggcgc cgccggcaac cagcgccatg cctccggcga
    31861 tctcggccgc gtccaacgca ccggcgaagc tcgacggtgc ggcatacggg ttgggcaacg
    31921 tggggtacca cacgaggccg gcaggatcgg ccgctccggc gatgcagatc aggaccgcgc
    31981 cgatgctggc gaggatgacg gcgagccgtc cgtacgggcg gggaacgcgg ccatccggga
    32041 acaggaggcc gatcgcgatg gccatcacac cgatgaccgg tgccgagaac gacgacacga
    32101 cccacgcgac catcagcgtc aacggcggtg cggcgcgata ggcgccgctc gccgcgtcga
    32161 cgaggagcgc ggtcggcgcc atcgacgcgt agacgatgcc gatggccagg agcagccagc
    32221 cgatggcgtt gcccgggacg cgcgagacca gcagccagcc ggcgccgacg tagctgagcg
    32281 ccgcggccat tccgatcgcg atgttgatcg gaccgcgcgc gaagctggtc ggaagtgccg
    32341 ggaagccctg cgtggcccag atcagcgcag cggcgaggac cgccgccacg gcgctcacgg
    32401 cgagcatgcc gccggcgacc gggagccacc gcgcggtccg cgaggtcacg gcggcgcctc
    32461 gcgtgccacg gcctccagat cggccaggac ggcgtcgagg tttgccggtt cggcggtgac
    32521 gtccaggacg gcctcgcgga acgcggtgct caggcccggt gccatgcgat cggacgcgtc
    32581 gaaccggaag gcctccgcgg ccgtcaacat ctcggccgcc cgagcactga cgtgatccgg
    32641 gtaatcggtc acgccgaagt tcacggacaa cgtgccgccc tgcgcgaccc acagcgactg
    32701 ggcctcgtcc gataccaggt agcgcatcag ctccgcggcg gccgcactgt cggtcaggac
    32761 gccgagcagg tcacctccgc cgagcacccc atcggtctcc gcggacgcca tggttgggaa
    32821 ggggaagaag tcgaagtcgc ggcccggctc tcggtcgccc cccgaggtga ggaaggcggg
    32881 catgaagctg ccctggtgga ggaagaggca tcccggcgga tcggcgaaca gcggctctcc
    32941 ggcgtcccgg aagttcgtct cgagcgtctg cagccgtcca ccggcgacgg atcgctccgc
    33001 cacgacctgt ccgtagagct cgaagccgcg ccggatctcg gcggacgtcc acgggagatc
    33061 cccggcaacc cacgcgtcgt acgcgtcgac gccggactcg tggagcacga actgctcgat
    33121 cagatccgtg cccggccatc ccgaggtctc ctcggacgcc agcccgacgc accactcgtg
    33181 cgtctcgcca ccggatgcgc gggcggccat ctgctgcagc tcgtcccacg tccgcggaga
    33241 cccgagcgtg aagaccgagg ggttgtacca gatcatgccc ttgaggctgg tgcggacgaa
    33301 gacgccaacc aggtggccgt cggcggtgcc cagctcgagg aacgtcggcg ccacgcgaga
    33361 ccgatacgcc cccaggtcga ggatctcggc gaggtcgcgc agttgtccgg cggaggcgag
    33421 ctccgccagg tgggccggtc cttccaggcc ggcgagatcg ggcggatgac cgctcgcaag
    33481 gcgctcggcc aggacgccgc ggaggtcgcg cgtctcctcg tacgtgacct caacccccgt
    33541 ccggtcctcg aacggagcga cgacggcccg gaaggcctcg acctcgtccc cggtccagct
    33601 cccgagcacc gagacctcgc cggtgagtgc cggctgcggc gtcgagcacc ctgccgcggt
    33661 cagggccatg gccatcacgg cgaccgccac gatcggcgcg ccaggcagac gcgacccccg
    33721 ctcaggcgac gcgggcatga accaatggtc cgtggttcga tcggtggcgc cagtgccgaa
    33781 tgcccccgct cacggcggac gatggaagac gcacgggcca atcccgacgg ctgagggacg
    33841 gccggcactt ggcagtcacg cacgtcgcgc gatcctgaac gggttcggca ccaggggaca
    33901 cgaggcgtgg tgcggctcgc cggtttcgcg tgggtcatcg cggcggtcgg cacggcggcg
    33961 ctgacgtcgt acctggtatc ccgcgcgtcg gacccatgcg ccgcggaatc cgcgtcctgt
    34021 ccggcggtgg ccgcggaggc gatccgggcg ctgatgtggc cagcctgggc ggtcctcctt
    34081 gttcccggtt acctcctcgt ctcgcgaccg accccggcgc gtgcgcgggc cgcggcgctc
    34141 ttcgggctgg tggtcgggcc gatcaccgcg gcggtcatca tccagctggg tcggtccgca
    34201 gcggcaggcg ccggctggga ggcgcgtggt gtcgcgttcg gagccctgct cgtcgttccg
    34261 ggactcctca gcgcggtcgc gtcgcgggac accgaccagc gcgcgtcccg gaaacggttg
    34321 cctgcgcgcc acgggcacac ctcacccggt ggatgaggcg tgcccagccc gcgagaagct
    34381 acgcggagcc gcccgggatc gccgcggcga tcgcggcgtc cagctcgaag aactggccga
    34441 tcggcttcag cgtgccgccg ccggtgtcgt acgagccgac caggtagacg cccatcgact
    34501 gctaccagta gagctgcacg atggcatcgc tgtccggcgg gcggaacgtc gcccactcgc
    34561 gacggtcgct cgtgcgaatg tccgtggcct cgagcttgcg gaggcgccgc gccaatgcga
    34621 gcgtccgttg ccgatcggcg tccggcgact ggttgaatgg ctgggacctg tggcacgccg
    34681 tggccacggc ggcgaggagc tcatccagac cgaacggctt cgcgagcacg gcggcgaaat
    34741 cggcggcttg cgctcggtcg gactcggcct cccgagcctc tcgcacgtcc gcggcgtgcg
    34801 cactgaacat gatcgtgggg atcgatcggt cgcgggcggc gaggtatgcc gcatcggccc
    34861 agctcgatcc gtactcggtc tgggagctgc c
//
LOCUS       DASTYP010000097         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_262324, whole genome shotgun sequence.
ACCESSION   DASTYP010000097 DASTYP010000000
VERSION     DASTYP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262324"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VFV59_04735"
     CDS             <1..960
                     /locus_tag="VFV59_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5325171.1"
                     /translation="ADRYATAASVSAYAFPAGAPAAFLATGVNFPDALGGIAAAATQH
                     APVLLVRGALIPATIGVELSRLDPDHAWLLGGEAVVGIGVAKTVQRMFGTCWSGNKPA
                     AGSAQVLTRIPDAPNQVALTFDMGGRMTPALDIVAFLVEHQVCATIFPTGAASQTADG
                     AAVLAALRAHPELFEVGNHTVNHCHLVVGGDGPSCPATPPSDAVITQELAGAEALVSA
                     GFGQSTAPYWRPPYGESDAHVRAVAASAGYTKTFMWDIDTIDWDAATTASQIVARVVP
                     NAVSGSIVLMHLGGYATLDALPSVVSQLRGRGFQLTTASDMFD"
     gene            complement(1138..1791)
                     /locus_tag="VFV59_04740"
     CDS             complement(1138..1791)
                     /locus_tag="VFV59_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5325172.1"
                     /translation="MSARVRVLEHARDRARATRQSTGREIREARVAAGLRLRDLGGVV
                     GRSEAWVSRVERGLVPRVSVAELTVLAAAAGLRLWVATYPGERLIRDAPQLDLLHRIR
                     ETIGTGWSWSYEVVARDRRGLRAADAVIRRGREVVMIEAFTRFTDAQAQIRAVHIKAR
                     DLGLERVVLVVKASHTNRRALLAAREIVEASFPLRTRAVLAALRAGRAPEANGIMVL"
     gene            complement(1837..>2403)
                     /locus_tag="VFV59_04745"
     CDS             complement(1837..>2403)
                     /locus_tag="VFV59_04745"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEU5325173.1"
                     /translation="AEIEPLEGRLTVAVASAPASGRPTAEAPVRIGPEDVSVEVRGAG
                     NATRQITHIITPEFPADRLEVVEVYTPSGNWSSWPPHKHDTDDMPREAVLEEVYSYRF
                     RRPEAWGVQRLYGGSADGLWAVRDGETVIVADGYHPFVATHGDDAYYLNALAGDIRTM
                     ACSYDPALDHVREAWASMAPDPRVPLVR"
BASE COUNT          356 a          897 c          801 g          349 t
ORIGIN      
        1 gcggatcgat acgccaccgc cgcatcggtc tccgcctacg cctttcccgc tggcgcaccg
       61 gccgcgttcc tggcgaccgg ggtgaacttc cccgatgccc tcggcggcat cgccgccgcc
      121 gcaacccagc acgcgcccgt cctgctggta cggggggctc tcatcccggc cacgatcggc
      181 gtcgagctct cgcgcctcga tccggatcac gcctggctgc tgggcggtga ggccgtcgtc
      241 ggcatcggcg tcgcgaagac ggtgcagcgg atgttcggca cctgctggtc gggcaacaag
      301 cccgcggccg gatcggcgca ggtgttgacg cgcatcccgg atgcgccgaa ccaggtggcg
      361 ctgaccttcg acatgggcgg gcggatgacc ccggccctcg acatcgtcgc cttcctggtg
      421 gagcaccagg tctgcgcgac catcttcccg acaggcgctg cctctcagac ggccgacggt
      481 gccgcggtcc tcgcggccct tcgcgcgcac cccgagctgt tcgaggtcgg caaccacacc
      541 gtgaaccatt gccacctggt cgtcggcggg gacggtccgt cctgtccggc cacgccaccg
      601 tccgacgccg tgatcacgca ggagctcgcc ggtgccgagg ccctcgtctc ggccggcttc
      661 gggcagtcga cggcgcccta ctggcgcccg ccgtacggcg agtccgatgc ccacgtccgc
      721 gcggtggcgg cctcggccgg ctacaccaag acgttcatgt gggacatcga caccatcgac
      781 tgggatgcgg cgaccaccgc gtcgcagatc gttgctcgcg tggtgccgaa cgcggtatcg
      841 gggagcatcg tgctcatgca cctcggcggg tacgccaccc tcgacgcgct gccctccgtc
      901 gtgagccagc tccgtggccg cgggttccag ctgaccaccg cctcggacat gttcgactga
      961 tttcggggca ccgatgtgtc gcttgctccc gcagcaagta actgccaaga agggcgtcat
     1021 caaggcccgt tcttcgccgt ggcttgcggc ccgtgcaagc cagcgccgaa ttgcgctgct
     1081 tgccgggagt tacttgcagt ccacgcaagc gccgcgagtc gccgcgggca cacggcccta
     1141 cagcaccatg atcccgttgg cctccggggc tctgcccgcc cgcagcgcgg cgagcaccgc
     1201 gcgcgtccgc agcggaaacg acgcctcgac gatctcacgc gccgcgagga gtgcacgccg
     1261 attggtgtgc gacgccttga cgaccaacac aacgcgctcg agcccgagat cgcgcgcctt
     1321 gatgtgcacg gcgcggatct gtgcctgcgc atcagtgaag cgggtgaacg cttcgatcat
     1381 cacaacctcg cgaccgcgac ggatcaccgc gtcggccgcc cgaaggccgc gccggtcgcg
     1441 cgccacgacc tcgtagctcc acgaccagcc ggtaccgatc gtttcgcgga tgcgatggag
     1501 caggtcgagc tggggcgcgt cccggatcag gcgctcgccg gggtacgtcg cgacccatag
     1561 tcggaggcct gcagcggcgg cgagcacggt cagctccgcc accgagacgc ggggcaccaa
     1621 cccgcgctcg acgcgcgaca cccaggcctc ggaccggcca accaccccac cgaggtcgcg
     1681 aagccggagc ccagcggcaa cgcgagcctc gcgaatctct cgacccgtcg actgacgggt
     1741 cgctcgggcc cgatcacggg catgctcgag aacgcggacg cgcgcggaca cccggcgagg
     1801 atgcccctgc tcgcataccg aggtattacc gccgggttac cggaccagcg gaaccctcgg
     1861 atctggcgcc atcgacgccc acgcctcacg gacgtggtcg agggccgggt cgtaggaaca
     1921 cgccatggta cggatgtccc cggccagcgc gttgaggtag taggcgtcgt cgccgtgggt
     1981 cgcgacgaac gggtgatagc cgtccgcgac gatcaccgtc tccccgtcac gcaccgccca
     2041 cagcccgtcc gccgatccgc cgtacagccg ctgcacgccc cacgcctcgg gacggcggaa
     2101 gcggtacgag tacacctcct cgagcaccgc ctcgcgaggc atgtcatcgg tgtcgtgctt
     2161 gtgcggcggc cagctcgacc agttgcccga gggcgtgtag acctcgacca cctccagccg
     2221 atcggccggg aattcggggg tgatgatgtg cgtgatctgg cgcgtcgcat tgccggcgcc
     2281 gcgcacctcc acggacacgt cctccggacc gatgcgaacc ggtgcctcgg cggtcggacg
     2341 gcccgaggcc ggcgcactgg cgaccgcgac ggtcagccgc ccctcgagcg gctcgatctc
     2401 agc
//
LOCUS       DASTYP010000098         9857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_334377, whole genome shotgun sequence.
ACCESSION   DASTYP010000098 DASTYP010000000
VERSION     DASTYP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334377"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1871
                     /locus_tag="VFV59_04750"
     CDS             114..1871
                     /locus_tag="VFV59_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5325174.1"
                     /translation="MTTAQPTDRVNADLGAPPWLAHYEAGVPSEIEVMEVPIDDLLRR
                     TAQRAGERTALIFFDKRTGYRELDRAADRFAHVLASMGVAKGDRISIHLPTSPAFVIA
                     FMGIMRIGAVAVPMNPLFVERELAVLFEQTRPKVSVTVDLLVPRLDRVAAEHGADGGR
                     PGRLIVTGIQDSLPVPIKWLYPLKARREGRWNPAKHSERTPNLFRLLREAPGRPFPSA
                     AAPDDLAVLQPTGGTTGLPKCAMLTHRNLVANAQQTAAWFPAVGADDDVSILCPLPLF
                     HIYGMTVDMNYAVLVGGTLILIPRFDPEMVLKATKHRPRLFPGAPIMYQTLAHHPHVG
                     KYDLRSIEACISGAAPLAPEVQRDFEAVTGGRVVEGYGLTEASPVTHCNPVHGERRNG
                     TIGLPFPSTEARILDPQTGEPVPPGEVGELVIRGPQVMSGYWERPDETATTLRDGWLH
                     TADIATMSDDGFFTIVDRAKDLIIVGGINVYPREVEEVLVTHPAVEDAGVIGIPEERH
                     GEVPYAFVVLSPGRTASAEELIAHCRANLSRYKVPARIEFREELPKTMIGKVLRKELR
                     KQVDAVADRANAASDGGAG"
     gene            complement(1868..2410)
                     /locus_tag="VFV59_04755"
     CDS             complement(1868..2410)
                     /locus_tag="VFV59_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325175.1"
                     /translation="MTETPEAPRHDGPTEVVEDHRGELIERLSGGTATLLLAWLIGIG
                     AFSTLLASWIFIWLVTGERLGIGALFGGFGFYTALAGATGPCVLWLTGRAQGHTLRWF
                     LWTGVKIALVMIAIIIGVILIATLLLTGTLPGANALGAAVIMVIGAVILGLIWGVATW
                     AADGWIARARLDEPAEDRLD"
     gene            complement(2407..3564)
                     /locus_tag="VFV59_04760"
     CDS             complement(2407..3564)
                     /locus_tag="VFV59_04760"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5325176.1"
                     /translation="MTDRFETLAVHAGAEPDPQTGAVVPPIQMSTTFKQDGVGGLRGG
                     FEYGRSGNPTRAALEAAIAALENGTHGLAYASGLAATQNLLYLLEPGDGLLMSDDVYG
                     GTWRLADKVWRRYGIETTPVDLRDLDAVAAALNGDRPPKMVWIETPTNPLLKVVDISA
                     VARLSAVAGAITVVDNTFATPYLQRPLELGADLVLHSATKYLGGHSDVIGGLVVTRRD
                     DLAERLRFHQNAAGAVPSPFDCFLIMRGLRTLAVRMERASANALVLALWLSERGDVER
                     VHYPGLPDHPAAELVERQMRLPGAMLSVEVAGGEDAARRIAGATRLFTLAESLGAVES
                     LIEVPAPMTHASVADSPLAVPPSLIRLSVGIENVDDLRADLDAAFAGHGNA"
     gene            complement(3621..5168)
                     /locus_tag="VFV59_04765"
     CDS             complement(3621..5168)
                     /locus_tag="VFV59_04765"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU5325177.1"
                     /translation="MTHVAEPPASAQEGSSELERVNHWIGGGRVAGTSGRRGPVYNPA
                     TGEVAREVDFASVEEVDAAVRAAHEAFPAWRATSISRRTEILFRIRNLVDQHREEIAA
                     HLTREHGKVRTDALGEVARGLENIEFATGISHLLKGGFSEQASTGVDVYQLRQPLGVV
                     AGITPFNFPAMVPMWMFANAIACGNTFVLKPSEKDPSASVYLAELMKEAGLPDGVFNV
                     VHGDKVAVDRILEHPDIAAVSFVGSTPIARYIYETGTKNGKRVQALGGAKNHMIVLPD
                     ADIDMAADAAVSAAYGSAGERCMAISVVVAVGDVADPLVGAIEQRLPKIKIGPGHEEG
                     SEMGPLITREHRDKVASYLDSGVSQGATLRADGREHVLYDKESGGFFLGVSLLDNVSP
                     EMECYRNEIFGPVLEVIRVPNYPSALELVNSNEYGNGTAIFTRDGGAARQYQFDVNVG
                     MVGINVPIPVPVAYYSFGGWKASLFGDLHMYGPEGIQFYTRAKVVTARWPDPGTSSVD
                     LGFPRTR"
     gene            5280..5462
                     /locus_tag="VFV59_04770"
     CDS             5280..5462
                     /locus_tag="VFV59_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325178.1"
                     /translation="MDERERDQDSPPIDESTYGGSSSETGGDYDDAGPTIANPAGDTM
                     SEEGERGQRPPEAPST"
     gene            complement(5577..6479)
                     /locus_tag="VFV59_04775"
                     /pseudo
     CDS             complement(5577..6479)
                     /locus_tag="VFV59_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
     gene            complement(6517..7425)
                     /gene="lgt"
                     /locus_tag="VFV59_04780"
     CDS             complement(6517..7425)
                     /gene="lgt"
                     /locus_tag="VFV59_04780"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU5325179.1"
                     /translation="MIDPIALEIGPLQLHWYGIIIAAAVLIGGLLGTRVARWLNENPD
                     DGWSMLLLVMVLAVIGARLYHVIHLWDYYSQNPLLIPQVWNGGLGIPGGVAGGALGIF
                     LYTRANHLNAARWMDVFAPALLLGQVIGRLGNFVNQELYGPPISECGNGTFPPCLPWG
                     IQIDAAHRAGTPWDAVTYPVDTTFFVPMFAYEMVLNLIGMLVILWVIRRFGSRLFAGD
                     AVLMYIMWYGSVRTLLETYRVNNWTILGVPTAMWIGIIGVVLSAAWFWYRHRRGWGSP
                     MINPGPSPESSQPADDVRPQPEPTAG"
     gene            complement(7435..8715)
                     /locus_tag="VFV59_04785"
     CDS             complement(7435..8715)
                     /locus_tag="VFV59_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325180.1"
                     /translation="MEGTKRHDAPAEEEGARWLVMRHPDLRRYLAARFVVSTAVQIQT
                     VAVGWHIYTETGDPLHLGLVGLSQFLPFILLILPAGHVADQFDRRRVQLVTFSILALC
                     AAALLALAVAGTRDPLPVFGVMAVFGAARAFNHPTGQALLVNVATPALFPRAVAVNAS
                     LLQVATIIGPALGGAIVIAGVAPAYAVTLAMLLVGVVLIAGLRAGGRGEAVREPVSWS
                     SLLSGIRFVRGHPAVLGSISLDLFAVLFGGATALLPIYAGEILHAGPVGLGIMRAAPA
                     VGAAVLAAAVATRPIRRHAGRWMFGGVAAFGLATLVFGVSTHLVVSVAALAVMGAGDM
                     VSVYVRHLLVQLETPDEIRGRVSAVNAVFIGASNELGEFESGITASWWGIVPAVLVGG
                     AATLAVAAAWTRLFPVLWRLDRFPGQRESSAHPG"
     gene            8746..>9857
                     /locus_tag="VFV59_04790"
     CDS             8746..>9857
                     /locus_tag="VFV59_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325181.1"
                     /translation="MNHAAPGPPRLPLPLSQLITLSIYWLGIQTIWGGLNITIIPGRL
                     DDVSRETQGTLLAIVMLAGAVAPIVIQPTIGVISDYTVTRWGRRKPYIVIGSLLDVVF
                     LAGIAFSNDFVALVAFYFLLQVSSNFAQGPFQGYVPDLVPARQVGLASGLMGLMLTLG
                     TILGVGIAAVGGMVENVALATLALGLVEVATMVVLVLTVDEGSAAPRRTRSWREIALS
                     AWGRDVLHQRDVLWLLAVRLLFLGAYNATLIAVGYFRRSHGMAPADADGIVFVATAIV
                     GVTTALAAIPGGRLSDRLGRRSVIWIAGAIAAAGLLGVAAAPTPELAIAAWVPFGIGM
                     GLFLSADWALMADVIPKETAGRFMGILNAGTAMAGPV"
BASE COUNT         1497 a         3511 c         3472 g         1377 t
ORIGIN      
        1 cggcctgcgc cgcctgctgc gcggtgagcc ggtggaccgc atcgggctcg gccgtcgcgt
       61 tgccgcgacg gtcatcgccc gggcaggtta cccagtctga gggaggagga ccgatgacga
      121 ccgcccagcc caccgaccgc gtgaacgcgg atctcggcgc tccaccctgg ctcgcccact
      181 acgaggccgg ggtgccgtcc gagatcgagg tcatggaggt gccgatcgac gacctgctgc
      241 ggcgcaccgc ccagcgagcc ggcgaacgga cggccctgat cttcttcgac aagcgcaccg
      301 gctaccgcga gctggaccgt gcggcggatc ggttcgccca cgtgctcgcc tccatgggag
      361 tggcgaaggg cgaccggatc agcatccacc tgccgaccag tccggcgttc gtgatcgcct
      421 tcatggggat catgcgcatc ggcgccgttg ccgtgcccat gaacccgctc ttcgtggagc
      481 gcgagctcgc ggtgctcttc gagcagacgc ggcccaaggt cagcgtcacc gtggatctgc
      541 tcgtcccgcg cctggaccgg gttgccgccg agcacggtgc cgacgggggt cggccgggac
      601 ggctgatcgt caccggcatc caggactccc tgccggtccc gatcaagtgg ctctaccccc
      661 tcaaggcacg ccgcgaggga cgctggaacc cggcgaagca ttccgagcgc accccgaacc
      721 tgttccgcct gcttcgcgag gcacccggcc ggccgttccc ctcggccgct gcccccgacg
      781 acctggcggt cctgcagccc acgggcggga cgaccggact ccccaagtgc gccatgctca
      841 cgcaccgcaa cctggtcgcc aacgcccagc agacggcggc ctggttcccg gcggtcggcg
      901 ccgatgacga cgtctcgatc ctgtgcccgc tgccgctgtt ccacatctac ggcatgaccg
      961 tggacatgaa ctacgcggtg ctggtgggcg ggacgctgat cctcatcccg cgcttcgacc
     1021 ccgagatggt cctgaaggcc acgaagcatc ggccgcgcct attcccgggt gcgccgatca
     1081 tgtaccagac gctcgcgcac catccgcacg tcgggaagta cgacctgcgc agcatcgagg
     1141 cctgcatcag tggcgccgcg ccgctggcgc cggaggtgca gcgcgacttc gaggccgtca
     1201 ccggcggccg cgtggtggag ggctacggcc tcaccgaggc ctcaccggtc acccactgca
     1261 acccggtcca cggcgagcgt cggaacggca ccatcgggct gccgttcccc tcgaccgaag
     1321 cgcgcatcct cgacccgcag accggcgagc ccgtgccgcc cggagaggtc ggcgagctcg
     1381 tgatccgcgg cccgcaggtg atgagcgggt actgggagcg gcccgacgag accgccacca
     1441 cgctccgaga cggctggctg cacacggccg acatcgcgac catgagcgac gacggcttct
     1501 tcaccatcgt cgaccgggcc aaggatctga tcatcgtcgg cggcatcaac gtctacccgc
     1561 gcgaggtgga ggaggtgctc gtcacccatc cggccgtcga ggatgccggc gtcatcggca
     1621 tccccgagga gcgacacggc gaggtgccgt acgcgttcgt ggtgctcagc ccgggcagga
     1681 cggcgagcgc ggaggagctg atcgcccact gccgcgcgaa cctgtcccgg tacaaggtgc
     1741 cggcgcggat cgagttccgc gaggagctgc ccaagacgat gatcggcaag gtgctgcgca
     1801 aggagctgcg caagcaggtc gacgccgtcg cggaccgtgc gaacgccgcg tcggacgggg
     1861 gtgccggcta gtccaggcga tcctcggccg gctcgtccag gcgggcccgc gcgatccagc
     1921 cgtccgcggc ccaggtggcg acgccccaga tcaggcccag gatcacggcg ccgatcacca
     1981 tgatgacggc cgcgcccagc gcgttcgccc ccggcagcgt cccggtgagc agaagggtcg
     2041 cgatcaggat gacgccgatg atgatcgcga tcatgaccag cgcgatcttc acgccggtcc
     2101 acaggaacca gcgcagggtg tggccctgtg cccggcccgt cagccacagc acgcacgggc
     2161 cggtcgcgcc ggcgagcgcg gtgtagaagc cgaagccgcc gaacagcgcg ccgatgccca
     2221 gccgctcacc ggtgaccagc cagatgaaga tccagctggc cagcagggtc gagaaggcgc
     2281 cgatgccgat gagccacgcc agcagcagcg tggcggtgcc gccggacagc cgctcgatca
     2341 gctccccgcg atggtcctcg acgacctccg tcgggccgtc gtgccgtggc gcttccgggg
     2401 tctcggtcat gcgttcccgt ggccggcgaa ggcggcgtcc aggtcggcgc gcaggtcgtc
     2461 gacgttctcg atcccgacac tcaggcggat cagcgacggc gggaccgcca gcggcgagtc
     2521 ggcaaccgac gcgtgcgtca tgggagccgg cacctcgatc agcgactcga ccgcgccgag
     2581 cgactccgcg agggtgaaga gccgggttgc gccggcgatc cgccgcgccg cgtcctcgcc
     2641 cccggcgacc tcgacgctga gcatcgcgcc ggggaggcgc atctggcgct cgaccagctc
     2701 ggcggccggg tgatcgggca gtccgggata gtgcacgcgc tcgacgtcgc cgcgttccga
     2761 tagccagagc gcgaggacca gcgcgttggc gctggcccgc tccatccgaa ccgccagcgt
     2821 gcggaggccg cgcatgatca ggaagcagtc gaacgggctg ggcacggcgc cggccgcgtt
     2881 ctggtggaag cgcagccgct cggcaaggtc gtcgcgccgc gtgaccacca acccaccgat
     2941 gacgtccgaa tgtccaccca ggtacttcgt ggccgagtgc agcaccaggt ccgcgccgag
     3001 ctcgagcggc cgctgcaggt agggcgtggc gaacgtgttg tcgaccacgg tgatggcgcc
     3061 ggccaccgcc gacaggcggg caaccgcgct gatgtccacg accttcagca gcgggttggt
     3121 cggcgtctcg atccagacca tcttcggcgg ccgatctccg ttgagcgcgg cggccacggc
     3181 gtcgagatcg cgcaggtcca ccggcgtggt ctcgatcccg tagcgccgcc agaccttatc
     3241 ggccagccgc cacgtcccgc cgtacacgtc gtcgctcatg agcagcccgt cgcctggctc
     3301 cagcaggtac agcaggttct gcgtggcagc caggccggac gcgtaggcca gtccgtgcgt
     3361 gccgttctcg agcgccgcga tggccgcctc gagcgccgcg cgcgtcgggt tgccgctgcg
     3421 gccgtactcg aacccaccgc gcaggccgcc gaccccgtcc tgcttgaagg tggtgctcat
     3481 ctgaatcggc ggcaccacgg cgccggtctg cggatccggc tcggcaccgg cgtggaccgc
     3541 aagcgtctcg aatcggtcgg tcatggatac ctcagagagg cgcggagtat atcgggatat
     3601 cccccgcgcc gagggactcc ctatcgggtg cgcgggaagc ccaggtccac gctcgacgtg
     3661 ccggggtcgg gccagcgagc cgtcaccacc ttggcgcgcg tgtagaactg gatgccctcc
     3721 ggcccgtaca tgtgcaggtc gccgaacagc gacgccttcc agccgccgaa gctgtagtag
     3781 gcgaccggca ccgggatcgg cacgttgatg ccgaccatgc cgacgttcac gtcgaactgg
     3841 tactgccgcg cggcgccgcc gtcacgggtg aagatggcgg tgccgttgcc gtactcgttc
     3901 gagttgacca gctcgagggc cgatgggtaa ttgggcacgc ggatgacctc cagcacgggg
     3961 ccgaagatct cgttgcggta gcactccatc tcgggagaga cgttgtccag cagcgagacg
     4021 cccaggaaga agccgcccga ctccttgtcg tacagcacgt gctcgcgtcc atcggcgcgc
     4081 agggtggccc cctgcgagac ccccgaatcc aggtacgagg ccaccttgtc ccggtgctcg
     4141 cgcgtgatga gcgggcccat ctcggagccc tcctcgtggc cggggccgat cttgatcttc
     4201 ggcagccgct gctcgatcgc gccgaccagc ggatcggcga cgtcaccgac ggccacgacc
     4261 acgctgatgg ccatgcagcg ctcgccggcg gagccgtagg cggcggagac ggcggcatcg
     4321 gcagccatgt cgatatcggc gtccggcagg acgatcatgt ggttcttcgc accgcccagg
     4381 gcctgcacgc gcttgccgtt cttcgtgccc gtctcgtaga tgtagcgcgc gatcggcgtg
     4441 ctgccgacga agctgacggc cgcgatgtcg gggtgctcga ggatgcggtc gaccgccacc
     4501 ttgtcgccgt ggacgacgtt gaagacgccg tccggcaggc cggcctcctt catcagctcg
     4561 gcgaggtaga ccgatgcgga cgggtccttc tcagatggct tgagcacgaa ggtgttgccg
     4621 caggcgatgg cattggcgaa catccacatc ggcaccatgg ccgggaagtt gaacggcgtg
     4681 atgccggcca cgacgcccag gggctggcgg agctggtaca cgtcgacccc ggtcgaggcc
     4741 tgctcgctga agccgccctt gagcaggtgc gagatgccgg tcgcgaactc gatgttctcg
     4801 aggccgcggg cgacctcgcc cagcgcatcg gtccggacct tgccgtgctc ccgggtcagg
     4861 tgcgcggcga tctcctcgcg atgctggtcg accaggttgc ggatgcggaa caggatctcg
     4921 gtccggcggc tgatgctggt ggcgcgccac gccgggaacg cctcgtgcgc tgcgcgaacc
     4981 gcggcatcca cctcctcgac cgatgcgaag tcgacctcgc gcgcgacctc gcccgtggcc
     5041 gggttgtaga ccgggcctcg acggccgctc gtgccggcca cgcggccccc gccgatccag
     5101 tggttgaccc gctccagctc gctcgaccct tcctgggccg aggccggcgg ctctgcgacg
     5161 tgcgtcatga ggctcctcgt cgtgctccct cggtgatcag aacggcaacg ataccgcgcc
     5221 ggccggttcc cgtcaggaac gccggactgc tggcacgcag gctgcgagtg gccgccagca
     5281 tggacgaacg tgagcgcgac caggactccc ctcccatcga cgagtcgacg tacggcggct
     5341 cctcatccga gacaggcgga gactacgacg atgcggggcc gaccatcgcc aaccccgcgg
     5401 gcgacacgat gtcggaggaa ggggagcgcg gccagcggcc accggaggct ccttcgacct
     5461 gacccgctct acgacggctg cgggatctgc tcgccgcgca gcacgacctc ggcacgggtg
     5521 gtccgccagt cgtacgacgt caccgactcg tcggcgccca acacaccgac cgaacgcagc
     5581 cactcatcgt tgtagagctt cccgacgtag ttgcggcccg agtcgggcag caggacgacc
     5641 atcagcgcct cgggatcgtc gagatcgcgc gcgacctgga gcgcggccca cagcgcggtc
     5701 ccgcacgact cccccatcag gatgccctcc gccgccgtgg cggcgcgcgc catcagcatc
     5761 gagtcgcggt cgctgacgcg gacgatcaca tcgcagatcc gcgtgtcgta cgtttcgggc
     5821 cagaagtcct ccccgatccc ctcggtgagg tacgcgcgcg gcgtgtcgcc ggagtacacg
     5881 gagccggcgg ggtcggcccc gatgatctcg atggccggat tgcgctcctt caggtagcgc
     5941 cccgctccgg tgacggtgcc gccggtcccg acggcgatca ccaggtgcgt gagacggccc
     6001 tcggtcgcct cccagatctc cggtccggtg gtgaggtagt gcgcctcggg attcgccgca
     6061 ttgtggtact ggtccggctt ccagccgcct ggggtctcgc gcgccaggcg atcggatacc
     6121 gagtagtacg agcgcgggtc ctcgggggcg acgtcggtgg ggcacaccac gacctccgcg
     6181 ccgtacgcgc gcaggagagc tcgcttctcc tcggactgct tgtcggccat cacgaagatg
     6241 caccgatacc ccttcacggc cgccgcctgc gccagcccga ccccggtgtt gccgctagtc
     6301 ggctcgacga tcacgccgcc gggcttgagc agcccggccc gctcggccgc ctcgatcatc
     6361 ggcatggcga tgcggtcctt gaccgagcca ccggggttca tgtgctccag cttcgccgcg
     6421 atgcgcgggc ggacatcggc cgccaagcgg tccagcgcga cgagcggcgt gcgcccgatg
     6481 gcggcggtga cgtccggcag gatcccgccg cgctcgtcag ccagcggtcg gctccggctg
     6541 cggccgcacg tcgtcagcgg gctgcgacga ctccggtgac ggccccgggt tgatcatcgg
     6601 cgagccccag ccgcggcggt ggcggtacca gaaccaggca gccgacagga caaccccgat
     6661 gatgccgatc cacatcgcgg tcggcacgcc caggatggtc cagttgttga cccggtaggt
     6721 ctcgagcagg gtgcggacgg agccgtacca catgatgtac atcagcaccg cgtcgccggc
     6781 gaagagccgc gagccgaatc ggcggatgac ccacaggatc accagcatgc cgatcaggtt
     6841 gagcaccatc tcgtacgcga acatcggcac gaagaaggtc gtgtcgaccg ggtaggtcac
     6901 cgcgtcccac ggggtgccgg cgcggtgagc ggcgtcgatc tggatgcccc acggcaggca
     6961 gggcgggaac gtgccgttgc cgcactcgct gatgggcggc ccgtacagct cctggttcac
     7021 gaagttgccc aaccggccga tgacctggcc gagcagcagc gccggcgcga agacgtccat
     7081 ccagcgcgcg gcgttgagat ggttcgcccg ggtgtagagg aagatgccca gcgccccacc
     7141 ggccacgccg cccgggatgc cgagcccgcc gttccacacc tgcgggatca ggagcgggtt
     7201 ctggctgtag tagtcccaca ggtggatgac gtggtagagc cgggcgccga tgaccgccag
     7261 caccatgacg agcagcagca tcgaccagcc gtcgtccggg ttctcgttga gccagcgggc
     7321 gacccgggtg ccgagcaggc cgccgatcag caccgccgcc gcgatgatga tgccgtacca
     7381 gtgcagctgc agcggcccga tctcgagcgc gatggggtcg atcaccggcc gattctagcc
     7441 cggatgcgct gacgactccc gctggccggg gaatcggtcc agacgccaca gcacggggaa
     7501 gaggcgcgtc cacgccgccg ccaccgccag ggtcgccgcg ccgccgacca ggacggccgg
     7561 gacgatgccc caccacgagg cggtgatgcc ggactcgaac tcgcccagct cgttggaggc
     7621 gccgatgaag acggcgttca cggcgctgac gcggccgcgg atctcgtcgg gggtctcgag
     7681 ctggaccagc aggtggcgca cgtagacgct gaccatgtcc ccggcgccca tcacggccag
     7741 ggccgcgacg ctcacgacga gatgcgtgga cacgccgaag acgagcgtcg ccaggccgaa
     7801 ggccgcgaca ccgccgaaca tccagcggcc ggcgtggcga cggatggggc gggtggcgac
     7861 ggccgccgcg aggaccgcgg ccccgaccgc gggtgcggcg cgcatgatgc cgaggccgac
     7921 cggccccgcg tgcaggatct cgccggcgta gatcggcagc agggcggtgg cgccgccgaa
     7981 cagcacggcg aacaggtcga gtgagatgga gccgaggacg gcggggtggc cccggacgaa
     8041 gcggatgccg gacaggagcg agctccacga caccggctcg cgaaccgcct cgccccggcc
     8101 gcccgcgcgc agcccggcga tcagcacaac ccccaccagc agcatggcga gcgtgacggc
     8161 gtaggccggc gccacaccag cgatgacgat ggcgcctccg agcgccggcc cgatgatcgt
     8221 ggccacctgg agcagcgagg cgttcaccgc cacggcgcgc gggaagagcg ccggcgtcgc
     8281 gacgttgacc agcagcgcct gcccggtcgg gtggttgaag gcccgcgccg cgccgaagac
     8341 cgccatgacg ccgaacaccg gcagcggatc ccgggtcccg gcgacggcga gcgccaggag
     8401 cgccgccgcg cacagcgcca ggatcgagaa ggtcacaagc tggacccggc gccggtcgaa
     8461 ctggtcggcg acgtgcccgg cggggaggat cagcaggatg aacggcagga actgggagag
     8521 gccgaccagg cccaggtgga gcgggtcgcc ggtctcggtg tagatgtgcc agccgacggc
     8581 gacggtctgg atctggaccg cggtcgagac gacgaagcgc gccgccaggt agcggcgcag
     8641 gtcgggatgg cgcatgacca gccaccgcgc gccttcctcc tcggcgggag cgtcgtggcg
     8701 cttcgtccct tccatcggcg gggagcctag cgtagattcc gccccgtgaa ccacgccgca
     8761 cccgggccac cgcggctacc gctaccgctc tcgcagctga tcacgctgtc gatctactgg
     8821 ctgggcatcc agaccatctg gggcggcctc aacatcacga tcattcccgg ccgcctggac
     8881 gatgtcagcc gcgagacaca gggcacgctg ctcgcgatcg tgatgctggc cggggccgtc
     8941 gcccccatcg tcatccagcc gacgatcggg gtcatcagcg actacaccgt cacacgttgg
     9001 gggcggcgca agccgtacat cgtcatcggc tcgctgctcg acgtcgtgtt cctggccggc
     9061 atcgcgttca gcaatgactt cgtcgccctg gtcgcgttct acttcctgct ccaggtcagc
     9121 tcgaacttcg cgcagggtcc gttccagggg tacgtgccgg acctcgtccc ggcgaggcag
     9181 gtgggcctgg ccagcggcct gatggggctg atgctgacgc tcgggacgat cctgggcgtc
     9241 ggaatcgcag cggtgggcgg catggtcgag aacgtcgcgc tggccacgct cgccctcggc
     9301 ctggtcgagg tcgccaccat ggtggtgctg gtgctcaccg tggacgaggg atcggccgcc
     9361 ccgcgacgca cgcgttcgtg gcgcgagatc gcgctgtcgg cctggggacg cgacgtgctg
     9421 caccagcgcg acgtcctgtg gctgctggcc gtccgcctcc tcttcctggg cgcgtacaac
     9481 gccacgctca tcgcggtcgg ctacttccgg cgctcgcacg gcatggcgcc ggcggacgcc
     9541 gacggcatcg tcttcgtcgc gaccgccatc gtcggcgtga ccacggcgct cgccgccatc
     9601 ccgggcggac ggctgtcgga ccggctcggg cggcgcagcg tcatctggat cgcgggcgcc
     9661 atcgcggccg cggggctgct cggcgtggcg gcggcgccga ccccggagct cgccatcgcc
     9721 gcgtgggtgc cgttcggcat cggcatgggg ctgttcctct cggccgactg ggcgctgatg
     9781 gccgacgtca ttcccaagga gacggccggg cgcttcatgg gcatcctcaa cgcgggcacc
     9841 gccatggccg gaccggt
//
LOCUS       DASTYP010000099         5822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1719278, whole genome shotgun sequence.
ACCESSION   DASTYP010000099 DASTYP010000000
VERSION     DASTYP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719278"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV59_04795"
     CDS             complement(<1..183)
                     /locus_tag="VFV59_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325182.1"
                     /translation="MDNDDRDRRDWPSEDEATRPAAEPDGAAELTDAPAPTEPGDLAE
                     PRPWDEPAASPPTPDAT"
     gene            complement(229..978)
                     /gene="purQ"
                     /locus_tag="VFV59_04800"
     CDS             complement(229..978)
                     /gene="purQ"
                     /locus_tag="VFV59_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5325183.1"
                     /translation="MTALVAARPRVAVVVFPGTWSERDFLHVAQSVLGWEAWTVWHTA
                     TDLGSPDAVVLPGGFAHGDHLRTGAIARFSPVMGPVEAFAAAGGPVIGSCNGFQVLTE
                     AGMLPGALMRNDHLEFRCDWQWLRVEHPASAAAWLGDLADGEAIRLPISHGEGRYVAD
                     AATLDALEAGGRVVLRYADSAGDVTSAANPNGSERGIAGIVNERGNVLGLMPHPERAA
                     EAEVGGTDGLRLFRSLERWLQHQPAAAERRL"
     gene            complement(975..1259)
                     /gene="purS"
                     /locus_tag="VFV59_04805"
     CDS             complement(975..1259)
                     /gene="purS"
                     /locus_tag="VFV59_04805"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU5325184.1"
                     /translation="MRWLAEVHVALKSGIADPEGQTIAAALRSLGYASVGDVRSGKLL
                     RIGFDADDPPTAEATVAEMCRRLLANPVTETATWELRADEQQDAEPAMPS"
     gene            complement(1270..2154)
                     /locus_tag="VFV59_04810"
     CDS             complement(1270..2154)
                     /locus_tag="VFV59_04810"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5325185.1"
                     /translation="MTITTTRLEGLPLVHRGKVRETYEIDPQHLLLVATDRLSAFDVV
                     FDQPIPDKGAVLTRLSAWWFSELADLGPTHFVSADVADLPEPARTPEMAPRSMVVRRA
                     QRVDVECVVRGYLAGSGWAEYRTQGTVCGHRLPDGLREADRLPEPIFTPSTKAEVGHD
                     ENISRETLAEMVGAELARELEDRSIALYQAGARRAEEVGLILADTKFEFGHIDGRLAL
                     IDEVLTPDSSRFWDADRYEPGMSPPSFDKQFVRDFVADSGWNKEPPAPELPADVIAGT
                     RDRYVAAFERLTGRAWEG"
     gene            complement(2151..2663)
                     /locus_tag="VFV59_04815"
     CDS             complement(2151..2663)
                     /locus_tag="VFV59_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5325186.1"
                     /translation="MTPDLSIEVQLDLALRLTVGLILGAIIGFERELHRQPAGFRTHS
                     LVSLGAALFTIVSAYGFVGDQVDPTRIAAQIVSGIGFIGAGTILQHRGHIRGLTTAAS
                     LWSVAAIGTAAGARLYVVAIIGTVLILIILSILDRVETMARHRLQLPPEGFILPDDQG
                     PDDGKSEDLP"
     gene            complement(2660..3562)
                     /locus_tag="VFV59_04820"
     CDS             complement(2660..3562)
                     /locus_tag="VFV59_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325187.1"
                     /translation="MAGWVAAATGAGDDVAERPGLWVPAALGWIGTVGWIPLVAGVAR
                     PPTTSELTYAGQAIFSSGAWPWNAVALGVAAVGVLVVAMGAAAYGDAWLIRRLAGRAG
                     PSLDEVEGAAAVQLVAVAPAIMALIAASTTLVVIGPQEFNAPSGAVPPILRVALRLWP
                     FLAAAVLLALVGSAWGAAAVRWGLATDRFARSALWAPRRLLEAAPGSVLQAVIGPIVG
                     LVYLVVATALLRVLWAPIEVQLGSGIDAAPLLLLVGFVAIWLCLVLGGGALRAWSCAT
                     WSRLLAAEPLPDPQPATRERPIDR"
     gene            complement(3575..4891)
                     /gene="purB"
                     /locus_tag="VFV59_04825"
     CDS             complement(3575..4891)
                     /gene="purB"
                     /locus_tag="VFV59_04825"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5325188.1"
                     /translation="MIDRYALPELRDIFSERHKLDLWLRIELLAVEAWRDAGLVPHDD
                     WERIRAAAGEVDAERAREIEQESQHDVIAFLRSVTERLGPEGRWLHYGLTSSDVLDTA
                     TAVVLRDAARVIERELHRLVEVVRRLALQYRTTPMVGRSHGIHAEPITFGFKAAGWYA
                     ELRRNVERLARAREAIATGSISGAVGTHANVTAEVERHVLDGLGLQPDPAPTQVVSRD
                     RHAELLTTLAILGGTLERIAVEIRHLQRTEVGEAFEPFGSGQQGSSAMPHKRNPVLAE
                     RISGLARLLRADALVGLENMALWHERDISHSSAERFVFERSLGVAAYATRTLADLLDG
                     LEVDAERMRSNLEQLGGMVYSEALLLAMVAKGADRQAAYRLVQGAARRGWAGETTFAD
                     ALRTDPAVAEWLAPEEIERAMDLDHHLAGIDATYRALRLAGDDAGE"
     gene            complement(4888..5385)
                     /gene="purE"
                     /locus_tag="VFV59_04830"
     CDS             complement(4888..5385)
                     /gene="purE"
                     /locus_tag="VFV59_04830"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011865032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU5325189.1"
                     /translation="MLSVVPTVAVLCGSRSDLGTLKGCFDVLDLYEIPWEASVISAHR
                     QADALHAYVAEAEAAGVKLFIGAAGLAAHLPGVLASLTAKPVIGVPLDGGALGGADAL
                     YAVVQMPPGVPVATVAIGSAGAKNAAHLAARILALGDNGIAQRVAAFRDDQAGKAELR
                     IDPRA"
     gene            5467..5691
                     /locus_tag="VFV59_04835"
                     /pseudo
     CDS             5467..5691
                     /locus_tag="VFV59_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005271362.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
BASE COUNT          789 a         2184 c         2081 g          768 t
ORIGIN      
        1 ggtcgcgtcc ggggtcggtg gcgacgcggc gggctcgtcc cacggtcgtg gctcagccag
       61 gtcaccgggc tcggtggggg caggtgcgtc ggtgagttcg gccgccccgt cgggctcggc
      121 ggcgggccgg gtggcctcgt cctcgctcgg ccagtcgcgg cggtcccggt cgtcgttgtc
      181 catgcgagtc atcgcctccc tgggggccgg ctcagaggtt ctggccgatc atagccgacg
      241 ctcggcggcc gccggctgat gctgcagcca gcgttccagc gagcgaaaga ggcgcagccc
      301 gtcggtgccg cccacctcgg cctccgccgc gcgctccggg tgcggcatca ggcccagcac
      361 gttgccgcgc tcgttcacga tccccgcgat cccccgctcg gagccattcg gattcgccgc
      421 cgaggtcacg tcaccggccg agtccgcgta gcgcagcacc acgcggccgc cggcctcgag
      481 cgcgtcgagg gtggcggcgt cggccacgta ccgcccctcg ccgtgcgaga tcggcaggcg
      541 gatcgcctcg ccgtcggcca ggtcacccag ccacgccgcg gccgacgccg gatgctccac
      601 ccgcagccac tgccagtcgc agcggaactc caggtggtca ttccgcatca gcgcgcccgg
      661 aagcattccg gcttcggtga ggacctggaa gccgttgcac gagccgatga ccgggccgcc
      721 cgcggcagcg aacgcctcga cggggcccat caccggcgag aatcgcgcga tggcgcccgt
      781 ccgcaggtgg tcgccgtgcg cgaagccgcc gggcagcacc accgcgtcgg gcgagccgag
      841 gtccgtcgcg gtgtgccaca ccgtccacgc ctcccacccg agcaccgact gcgcgacgtg
      901 gagaaagtct cgttcgctcc aggtgcccgg gaagacgacg acggccacgc gcggtcgggc
      961 ggcgaccagc gcggtcacga gggcatcgcc ggttcggcgt cctgctgctc gtcggcgcgc
     1021 agctcccacg tggccgtctc ggtcaccggg ttcgcgagca ggcgccggca catctcggcc
     1081 acggtcgcct cggcggtggg aggatcatcg gcatcgaagc cgatgcgcag gagcttcccc
     1141 gaccgcacgt cgcccacgct ggcgtagccg agcgagcgca gggcggccgc gatggtctgc
     1201 ccctccggat cggcgatgcc gctcttcagg gcgacgtgga cctcggcgag ccagcgcatc
     1261 ggttcgcgtt cagccctccc aggcgcgccc ggtcaggcgc tcgaaggcgg ccacgtagcg
     1321 gtctcgggtt ccggcgatca cgtcggccgg cagctcgggt gcgggcggct ccttgttcca
     1381 acccgaatcg gcgacgaagt cgcgcacgaa ctgcttgtcg aagctcggcg ggctcatgcc
     1441 gggctcgtag cggtcggcgt cccagaagcg gctggaatcc ggcgtcagca cctcgtcgat
     1501 cagcgccagc cgcccgtcga tgtggccgaa ctcgaacttc gtgtcggcca ggatgagccc
     1561 gacctcctcg gctcgccgcg ccccggcctg gtacagcgcg atcgaccgat cctcgagctc
     1621 acgggccagc tctgcgccca ccatctcggc gagggtctcg cggctgatgt tctcgtcgtg
     1681 gcccacctcg gccttggtcg acggggtgaa gatcggctcc ggcagccggt cggcctcgcg
     1741 cagcccgtcc gggagccggt ggccgcagac cgtcccctgc gtgcggtact cggcccagcc
     1801 cgaccccgcg aggtaaccgc gcaccacgca ctcgacgtcg acccgctgcg cgcgccgcac
     1861 gaccatggag cgcggcgcca tctccggggt ccgcgccggc tccgggaggt ccgcgacgtc
     1921 ggcggagacg aaatgggtcg gacccaggtc ggcaagctcg ctgaaccacc atgccgagag
     1981 gcgggtgagg acggcgccct tgtccgggat cggctggtcg aagacgacgt cgaaggccga
     2041 cagccggtcg gtggcgacca gcagcaggtg ctgcggatcg atctcgtacg tctcgcgcac
     2101 cttgccgcgg tgcaccagcg gcagcccctc gagccgggtg gtcgtgatgg tcatgggagg
     2161 tcctcgctct tgccgtcgtc gggtccctgg tcgtcgggaa ggatgaagcc ctcggggggc
     2221 agctgcaggc ggtggcgggc catcgtctcg acccggtcga ggatggagag gatgatcagg
     2281 atgagcaccg tgccgatgat cgccacgacg tacagccgtg cgcccgctgc cgtgccgatc
     2341 gcggccaccg accagaggct cgccgccgtg gtcaggccgc ggatgtgccc gcggtgctgg
     2401 aggatcgtgc cggcgccgat gaagccgatg cccgacacga tctgcgccgc gatgcgcgtg
     2461 ggatcgacct ggtcgccgac gaagccgtag gcgctgacga tggtgaacag ggccgcgccc
     2521 agcgacacca ggctgtgagt ccggaagccg gccggctggc ggtgcagctc gcgctcgaag
     2581 ccgatgatcg cgccgaggat gaggccgacg gtcaaccgca gggccaggtc cagctggacc
     2641 tcgatggaca gatccggggt catcggtcga tcggtcgctc ccgtgtggcc ggctgcgggt
     2701 cgggcaatgg ttcggccgcg agcagtcggc tccacgtcgc gcagctccac gcccggagcg
     2761 ctccaccgcc gaggaccagg cacagccaga tcgccacgaa gcctaccagc agcaggagcg
     2821 gcgccgcgtc gatccccgac ccgagctgca cctcgatggg cgcccacagc acgcgcagca
     2881 gcgccgtcgc cacgaccagg tagaccagac cgacgatggg gccgatgacc gcctgcagca
     2941 ccgagcccgg cgctgcctcg agcagccgcc ggggcgccca cagggccgac cgcgcgaagc
     3001 ggtcggtggc gagtccccat cgcacggcgg ccgctcccca tgcactcccg acgagcgcca
     3061 ggagcaccgc cgcggcgagg aacggccaca gccgcagcgc cacgcggagg atcggcggaa
     3121 cggcgccact cggcgcgttg aactcctgcg gcccgatgac gaccagggtc gtcgaggccg
     3181 cgatcagggc catgatggcc ggcgccacgg cgacgagctg caccgcggcc gcgccctcga
     3241 cctcgtccag ggacggtcct gcgcgtccgg ccaggcgccg gatgagccac gcgtcaccgt
     3301 aggccgcggc gcccatcgcc acgacgagca caccgacggc cgcgacgccg agggccaccg
     3361 cgttccacgg ccacgcgccg gagctgaaga tcgcctgccc ggcgtaggtc agctccgagg
     3421 tcgtcggcgg tcgcgccacg ccggcgacca gcgggatcca cccgaccgtg ccgatccagc
     3481 cgagcgcggc cggcacccac agccccggcc gttcggccac gtcgtcgccc gcgccggtgg
     3541 ccgctgcgac ccagcccgcc atgtgcggcg gtccctactc gcccgcgtcg tcgccggcga
     3601 gacgcagcgc ccggtacgtg gcgtcgatgc cggccaggtg gtggtcgagg tccatggcgc
     3661 gctcgatctc ctcgggggcc agccactcgg ccacggcggg gtcggtgcgc agggcatcgg
     3721 cgaaggtcgt ctcgcccgcc cacccccgcc tcgccgcgcc ctgcaccagc cggtacgcgg
     3781 cctggcggtc cgcgcccttc gccaccatgg ccagcagcag ggcctcggag tagaccatgc
     3841 cccccagctg ctcgaggttc gaccgcatcc gctccgcatc gacctcgagg ccgtcgagga
     3901 gatcggccag cgtgcgcgtc gcataggcgg ccacgcccag cgaccgttcg aacacgaagc
     3961 gctccgcgga gctgtggctg atgtcgcgct cgtgccacag cgccatgttc tcgagcccga
     4021 ccagcgcatc ggcacgcagc aggcgcgcca ggccggagat gcgctcggcg aggaccgggt
     4081 tgcgcttgtg gggcatggcg ctcgaaccct gctggcccga cccgaacggc tcgaacgcct
     4141 cgccgacctc cgtgcgctgc aggtgccgga tctcgaccgc gatccgctcc agcgtgccgc
     4201 ccaggatggc cagcgtggtg agcagctccg cgtggcggtc gcggctcacg acctgcgtcg
     4261 gcgccgggtc gggctgcaag cccagcccgt cgaggacgtg gcgctcgacc tcggccgtca
     4321 cgttggcgtg ggtcccgacc gcgccggaga tggagccggt ggcgatcgcc tcgcgggcgc
     4381 gcgccagccg ctcgacgttg cgccgcagct cggcgtacca gccggccgcc ttgaacccga
     4441 aggtgatggg ctcggcgtgg atcccgtggc tgcgcccgac catcggcgtg gtgcggtact
     4501 gcagggccag ccggcgcacg acctccacca gccggtgcag ctcccgctcg atcaccctcg
     4561 ccgcgtcgcg cagcacgacc gcggtcgcgg tgtcgaggac gtccgagctg gtcagcccgt
     4621 agtgcagcca gcggccctcg gggcccaggc gctcggtgac ggagcgcagg aacgcgatga
     4681 cgtcgtgctg cgactcctgc tcgatctccc gcgcgcgctc cgcgtccacc tcgccggcgg
     4741 ccgcgcggat ccgctcccaa tcgtcgtgcg gcaccaggcc ggcgtcacgc cacgcctcga
     4801 cggccagcag ctcgatgcgc agccacaggt cgagcttgtg ccgctcgctg aagatgtctc
     4861 gcagctcggg gagcgcgtac cggtcgatca cgcccgggga tcgatcctca gctcggcctt
     4921 gccggcctgg tcgtcgcgga acgcggccac gcgctgcgcg atgccgttgt cgccgagggc
     4981 caggatgcgg gccgcgaggt gcgcggcgtt cttcgcgccg gcgctgccga tggccacggt
     5041 cgcgaccggc accccgggcg gcatctgcac cacggcgtac agcgcatcgg ccccgccgag
     5101 ggctccgccg tccagcggaa ccccgatgac cggctttgcc gtcaggctgg ccagcacgcc
     5161 gggcaggtgg gcagcgagtc cggccgcacc gatgaacagc ttcacgccgg ccgcctcggc
     5221 ctcggcgacg taggcatgga gcgcatcggc ctgccggtgg gcgctgatga ccgatgcttc
     5281 ccacgggatc tcgtacaagt cgagcacgtc gaagcagccc ttcagggtcc cgaggtcgga
     5341 gcgggagccg cacaacacgg cgacggtcgg cacgaccgaa agcatacccg tccggctgcg
     5401 ccacgcgcgt acgatggctg gcgcgcgatg tgctgcccca cgtcgcgtcg catggacgcc
     5461 cgtagtgatc cgcgctacga cacggcggac ttctccgact cggcgcccgg gaacctgcgg
     5521 gcggactacg tgctgccaag tcggagtctg cacatcgtgg acgctggcgt cttctgtccc
     5581 gtccaggccg acccgctctc gtcgctcacc ggggtcttcc cgttcccgag ctcggatcac
     5641 cgcctcgtgt gggtggatgc cacggtgccc gggagcgcgc gcggaggctg accgtcagcg
     5701 gcccgagggt tgcgcttcca tctccaccgc ggagcgcacc tctcgggccg ccacgtcagt
     5761 ccggtactgc gacccctcca gcgccacctc ggctacgccc cggtacgccg catcgcgggc
     5821 gg
//
LOCUS       DASTYP010000100        16814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1500184, whole genome shotgun sequence.
ACCESSION   DASTYP010000100 DASTYP010000000
VERSION     DASTYP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16814
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500184"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFV59_04840"
     CDS             complement(<1..266)
                     /locus_tag="VFV59_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325190.1"
                     /translation="MNRRALGALLVAYGVFGIILTIAGMVVGFDLAARVERLTGTAGD
                     TLQAAARATRAAGDSFASVDSSLAESQTSADTAADLARDASATL"
     gene            complement(270..806)
                     /locus_tag="VFV59_04845"
     CDS             complement(270..806)
                     /locus_tag="VFV59_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325191.1"
                     /translation="MQHVHEHRFRPQPAGSLSLRQFVESLRRWTPDMRHVVQIALTRT
                     ENERRQYLQQFHAGHHDRQPGPLDSFIRWAYSDPRLDDIPLLLAEIELGPDAYAGALE
                     HEADVLVRERAHGKTPAQRVADYLALLGMENSERVRAAIASLLPPGTPDASVAATVRH
                     MRRRWLELYGDDPFFAED"
     gene            872..2425
                     /locus_tag="VFV59_04850"
     CDS             872..2425
                     /locus_tag="VFV59_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5325192.1"
                     /translation="MRRLTRPAALIAAGVLALVSTTSGAPIRAAEAPLRFLAGPAGTL
                     DPAFISDAADVQLLLQLYAGLTRLDEQGEPYPSLARSWEISDDGLTYDFTIRDDLAFS
                     DGTPLTAQDVRRSWLRLLDPTTAATAPDVLSVISGAPERLAGDADEDVVGIQVTDDTH
                     LRVTLRYPASYFLDILATPAAFVVPPRADATGTWQTPDDFVGSGPYAVDGIDGVDLVL
                     RANDEYVAGPPPIPEIRWVAAVDADAATAFQAGDVDLAQVAGWDATWIAYDRDLGPRL
                     HAAEPLTISYFGFDTTSPPFDDARVRRAFALALDRERLVPLSEGAESQAAASLVPPAI
                     WPDGFAPGIDHDLDEARRLLDEAGYEDRSDLGTIVVNGSGLDIGPAAAAWREELGVDV
                     RAEVMDFGDFLASLETDPPNLFTVNWIADYASPHALYGLLLAPDAASNWGRWDDDEFE
                     SLLEAAAAAPEDEVGRAYDEVESYVQEQAPVIPWSYGETWWLVADGLRGLGNLTIGLT
                     DFGRVSWDR"
     gene            2422..3756
                     /locus_tag="VFV59_04855"
     CDS             2422..3756
                     /locus_tag="VFV59_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEU5325193.1"
                     /translation="MILISRWLAAPALAVTLALLGAGSVAAFDGFGTSTADSTYDQGM
                     TFTIELTGAAPDRLELLLTTPGSDAVFVAPVPVRGASATYTWDTAVDHVTPNTPVTYQ
                     WRAIEGDEVTLSDPATLRYEDDRPGLDWQSANLGDATVHWYGDAETQARRFGELTAVG
                     VQRAEDLLGTTLAGPVDVFVYQSRDDFFGALGPGAREWTGAAAFTEIRTIFMWNSNSA
                     GDQAYLEQAMIHEVTHVVFADATDNPYHEPARWLNEGIATWSEDFDGADEAAIVRVEA
                     SGGGLFAFEAIEAQFPIGDRGGRLSYAQGTAMIDRIVDTYGEESLAKIAAAYRDGASD
                     AEALEAGTGVPAEQLYDDFYAAYGVDAPEPIAPDPIPASNVDRPEAGPLDPGGVDVPA
                     TPPPGDGAPGEHDGSAGGDPGAPLVVGALVVGAAAALAGALWLSRRAARRAA"
     gene            3753..4511
                     /locus_tag="VFV59_04860"
     CDS             3753..4511
                     /locus_tag="VFV59_04860"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEU5325194.1"
                     /translation="MSRRLPPKRRFVRPQWAISIGVALAAIGFVGAAQWNSSLARQEF
                     VTSAQRVLIGEAQDLQREQDDLRAQIEEAEDRVRSFQTTSAGSQAQLDQINSDLAEAR
                     LESGLVAVRGPGAVVEIADSTRQVPPGESVNNYLVLADDLRDIVTALWASGAEAITIS
                     GERLVSTSSIYSAGSAILVNTVPLVPPYRIEAVGPEGLHDRFLSNQAFVGRVGRRIES
                     YGLQFASEPRDDLELPAFVGNTRMRWGAPETAAD"
     gene            4511..5182
                     /locus_tag="VFV59_04865"
     CDS             4511..5182
                     /locus_tag="VFV59_04865"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEU5325195.1"
                     /translation="MAGRIAQLSLFVVAGLIGVLLVGQLRSQARPIELSNLSPQELSG
                     LVETLQQRNVELTNALAELREQVRDYERAEVQGQSTLELTSEELTRVRAFAGLEPVVG
                     QGIAIRMTGPFDPTAVNDLVHELRNAGAEAIAIDGIRITGRSVAVLGTSAIEIDGRPL
                     DETVEVLAVGYPTGLQSSIERPGGLKTYLEQSLDVTMSIETRRNLRLPATERDLAPTV
                     AEPVE"
     gene            5223..6671
                     /locus_tag="VFV59_04870"
     CDS             5223..6671
                     /locus_tag="VFV59_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5325196.1"
                     /translation="MKELIDRALDAAERGGASYTEVRIAERETEQLTVKNGALEAATS
                     NASGGFGVRVLVNGAWGFSSSRVTDAEEGERIAREALEIARASALAQRVPVELDDTPP
                     AVDSYRTPFEEDPFAVSLDEKLRILMEADEAMARVPGVAIREGVMFAGRERKTFASSA
                     GARIEQEIVEAGAGIEATAVNESEMQQRSYPNSFGGQMVTGGFEAVRALDLAGNAQEM
                     AELAVALLDAPQCPSGEMDVILDSTQVALQIHESCGHPTELDRVFGTEASFAGTSFLT
                     TDKLGQLRYGSELVNIDADATAPGGLGTFGYDDEGVPAQKVPLIDHGLLVGYLTSRET
                     APRIGRRSMGSARAWTWSHIPLIRMTNINLRPGDAGSLEDLIADTRDGIFMSINKSWS
                     IDDRRLNFQFGDQAGWLIKNGKRAQLVKNPTYTGITPQFWGSCDAICSADEWTLWGLP
                     NCGKGEPMQTGHVGHGAAPARFRNVKVGVGRW"
     gene            6665..8035
                     /locus_tag="VFV59_04875"
     CDS             6665..8035
                     /locus_tag="VFV59_04875"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5325197.1"
                     /translation="MVATRDEVSVLLDRVLEYAGGGVQAEALYLGEDAALTRFATNQI
                     HQNVRAHDASLQVRIIDGERVGVAATNRLDEASIRDVVERARAIAQRSAPNPRTAVLP
                     EPTDRDHESDLGYVAETAEASADVRAEGAAAVIAAGEAKKLETSGSYATSTMTMAVAN
                     TRGIRSLHRSTRAALLTVMMDGFASGAASGYANAVSPDVRDIDAAALGAEAAEKGDRM
                     RGAGELEPGEYEVILEEYAVAGLLEYLSFIGFSGLAHEEGRSFMELGKRVMGENVSIW
                     DDGHDVTGMPSPIDFEGVVRQRVDLIRDGVAAAVVHDAATGARAGTGSTGHALPAPNT
                     FGPMAMNLFMAPGTTPREELIAGVKRGVWVTRFHYINPVHPKKAILTGMTKDGTFLIE
                     DGRISRPLLNFRFTQSIPEAFSDVRAASRETKLLPGEFAGSARCPALHLGSFNFTGVT
                     AAEGAG"
     gene            8032..8247
                     /locus_tag="VFV59_04880"
     CDS             8032..8247
                     /locus_tag="VFV59_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEU5325198.1"
                     /translation="MTVAAPLDLRIGDRLRLRKEHPCGSREWDVVRLGADIGLVCAGC
                     GHRILMDRLDVERRFTAFVRRGDEGPA"
     gene            8251..9762
                     /locus_tag="VFV59_04885"
     CDS             8251..9762
                     /locus_tag="VFV59_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325199.1"
                     /translation="MAHIEVPTDAGDELAGAGTVFRNRAFVYLWSAQALSQLASNMVL
                     AALIATVVTTTGSLTANAVLILTFLVPAVLFSTLGGVLVERGDAKIIMLATNVVRAVG
                     TFLFILVAPTTSTAIVPLVYLINFGVATATAIFAPAELTSIPRIVDRRHLMAANSIFI
                     LTVNATFAIGFGVLGPLVLNVFGATAVYVIVGCMFALAAAAILPLPSVKPDHRPSAVG
                     DAAGRAVRELVDQLAEGVRFVRSHRRIAWSLTYLGIAASLIGVLGAIGPGFATDILRL
                     RPEDFFFIMGPAGLGAVVGILFLNSYGKGIPKRFVIDIGLVAMGFTLVALALVRPITS
                     LVAPAVAPIEDVFPTIAPITSLIALVVVIAVMAGVEYAFVAIPSQTALQEELPSDVRG
                     RIFGILNTLLSVASFLPVILAPAAADLINIFLRGAGIPIVMAILGALTLWAGIASWRR
                     NARAGLHQHETGQSPAEGVDSAPAPGTPERPAVDPPEAPHRHRRRRRQPPDAG"
     gene            9752..11083
                     /locus_tag="VFV59_04890"
     CDS             9752..11083
                     /locus_tag="VFV59_04890"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5325200.1"
                     /translation="MRARAISAVFLLVAAAVAWLTVTEPLAARVLQAGVAASLVYVAW
                     LAWRGSRLMRAAFRAARHPTGERMDAGGQLPPVTLVVPAWNEASVIAETVAHLASFAY
                     AGEDGRPRYDVLVVDDGSTDDTGALARSAASSAPHVQVMRREPQRDAPRTKGAVLAWA
                     MDGVQGEVVGVVDADTRVGPSFLQDAMLAWRGDPTAAALQVARRPHNAHRSWLTAAQA
                     EEQLMDLASQCGRRAVDGTAELRGNGMFVRVAALEAVGGWDATALTEDLELSTRLAIA
                     GEHVALAPHVAIGEEAIESIPPLWHQRVRWAEGSLRRLIQHAPAMLSGSQPLSRKADF
                     LAFAAEFLLPPLFATTTLASLVTVVMPQPADWTVPVSLALGYGLGVFLLALGGLSAVG
                     VRGLSLVGRAGRGSLFLSHWLLVVPWALVRILAGPGEGHFRSTPRFTPTTP"
     gene            11080..12708
                     /locus_tag="VFV59_04895"
     CDS             11080..12708
                     /locus_tag="VFV59_04895"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5325201.1"
                     /translation="MTASHATIVRGAVILRAEGGMLRTAEAIAIRDRRVVAAGSRAEV
                     EAAVPVAGTVHDVGPRVVLPGIHDFHVHLVGLARARAGVDLGAATRPAGLLDRLREAA
                     RATPPGAWVTGRGWTETQVVAAGLDRMADAVGSRAGFLASHDGHSAWASPAALSAARI
                     GPETPDPPGGRIERGAHGVPTGVLRETALDLVASHVPKLQGEALRPHLERVLRELASL
                     GITGASEAGDYTNEGGIGRDAAMGDSFSTLTDLGGLVDGRLRLSIGIPVDALPAAAAR
                     GFTTGAPLGESRTLRFGWAKEYADGALGSGTAALFSPRTCGSGGTGIMRVTDDELDAL
                     FALGRSARIGLAIHAIGDRAVAAVIDAATRAGPPATGVPSDRMEHVQLSRQQDRPRLA
                     GRLTASVQPIHAAADRDLVEACWDGRQDDAYAWRSIVEAGARLAFGSDTPVESVNPWL
                     GLHAAVHRRLPGDERDDWRPAEALTAVEAIAAYTTGPALALGREDEGHLEPGARADLA
                     VLDVDLPTLLAADERLGTVTSVLTMVDGDVIHEG"
     gene            12823..13074
                     /locus_tag="VFV59_04900"
     CDS             12823..13074
                     /locus_tag="VFV59_04900"
                     /inference="COORDINATES: protein motif:HMM:NF042901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325202.1"
                     /translation="MDRSQDEPEGASGRVPVGPGPASSFDPTVLDFGHYAGRSLEELA
                     SVDPEYLRWLARHPSGYRYRTEIGRVLASAVPRAGDWER"
     gene            13152..13691
                     /locus_tag="VFV59_04905"
     CDS             13152..13691
                     /locus_tag="VFV59_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5325203.1"
                     /translation="MTSTRPRPLSAAAPRRAGGATGPWRHILLACDGAAGTIAAGRRA
                     VELAARDGAALTIVLVRPAPATRAEPSVPPAADDIASLPEAIERVLAAATRAGIAAHG
                     EVRRGQSGAAILDVAREVDADLIIIGRHGPCIGTSFAVANCGYVVTHSDRPVLVVQPW
                     AREGDLLDAPLRPAAEEAS"
     gene            13811..14656
                     /locus_tag="VFV59_04910"
     CDS             13811..14656
                     /locus_tag="VFV59_04910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5325204.1"
                     /translation="MRAHEANVVVAAGSLLLVLVSMLREPAVPWLTWDSRVYWETSLA
                     PDLYANARVGALGAYLYSPAFAQLLAPAGLLPWPLFLFGWTFVSIRVAAWLCSGPIRR
                     HRALWLPLATLAILDIGAGNVNVLIGGAIVLALRWSGAWSAVLLTKVTPGMAVIWHLV
                     RREWRPLLSALAVTLLVVGISAVFEASAWVDWGTVLLSNATPHELSGDLAIPAAVRFP
                     VGLVIVAWGALTDRRWVVPIAAILFLPVIWPNTLALLLASAALWADAQPSPAGGAAVR
                     RTVPA"
     gene            14653..15819
                     /locus_tag="VFV59_04915"
     CDS             14653..15819
                     /locus_tag="VFV59_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEU5325205.1"
                     /translation="MNAQRLVWVDWLKGLVVLGVVAFHAAQPYVVTDWIVNATEDSYV
                     LSGLAGLGYLFAMPLMFLLAGVTGWIALERRTTWQYLSVRLLRLGLPLVVGLAFLSPL
                     ESWVGQLSYGETPDLPSFVARTWSELPPLTSPRWLGDVGHHLWFLGFLLGYVLLTIVP
                     LRWLRRRTPPSATRHVPAVVAMLGPIVPIAILQWLVRPPAPQYRDWADAAVWLVYFCI
                     GIGLAFDRAWLRPIARSGGWMTIPALLLAALLLPLGLDGVMRLESAPSLGVASLAYAS
                     ARTAIGWWLVCAVMALGMRWLTGAAWAAERAGEASLAFYVLHHPIVVVIAAAVAPWSV
                     GALAGFVVIFVSSVAVSAALYEGVVRRVGVLRSVFGVAEPPPERPLGPPQPEPI"
     gene            complement(15956..16387)
                     /locus_tag="VFV59_04920"
     CDS             complement(15956..16387)
                     /locus_tag="VFV59_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325206.1"
                     /translation="MLPTARRWTGLSLRARDITTVQGAAALIDDLGADLDRHLQHERR
                     PAERLRLLRETTNRITRVANDAIQAYQRASRAAASELTKPRGNVAAAMEARVILDAGR
                     SEILRALTAAQRRYPSDVDSDLPGTDDSISSRVPPGPPPPA"
     gene            complement(16510..16713)
                     /locus_tag="VFV59_04925"
     CDS             complement(16510..16713)
                     /locus_tag="VFV59_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU5325207.1"
                     /translation="MTTGTVKRVISERGFGFIAAPDGKEYFFHKDGLTSSLDFDRLVG
                     GESVTFEVEAGPKGPRAVQVQGA"
BASE COUNT         2198 a         6027 c         6119 g         2470 t
ORIGIN      
        1 tcgagcgtgg ccgacgcgtc gcgcgccagg tcggcggcgg tatcggccga ggtctgtgat
       61 tcggccaggc tggagtcgac gctggcgaac gaatcacccg ccgcgcgcgt ggcccgggcg
      121 gcagcctgca gggtgtcgcc ggccgtgccg gtgagccgct cgacgcgggc cgcgaggtcg
      181 aagccgacca ccatgccggc gatggtgagg atgatcccga agacgccgta cgcgacgagg
      241 agggcgccga gggcgcggcg gttcatcggt cagtcctccg cgaagaacgg gtcgtcgccg
      301 tagagctcca gccaccggcg gcgcatgtgg cggacagtgg cggccaccga agcatccggc
      361 gtgccgggcg gcaggaggct ggcgatggcg gcgcggactc gctcgctgtt ctccatgccg
      421 aggagcgcca ggtagtcggc gaccctctgt gctggcgtct tgccgtgggc ccgctcccgg
      481 accaggacgt cggcctcgtg ctcgagggca ccggcgtacg catcgggccc gagctcgatc
      541 tcggcgagca gcagcgggat gtcgtcgagg cgcgggtcgg agtacgccca gcggatgaag
      601 ctgtcgagcg gacccggctg ccgatcgtgg tgaccggcgt ggaactgctg caggtactgg
      661 cgtcgctcgt tctcggtgcg ggtgagcgcg atctgcacca cgtgacgcat gtccggcgtc
      721 cagcgccgaa gcgactcgac gaactggcgc agggagaggc ttccggccgg ctgcggccgg
      781 aatcggtgtt catggacgtg ctgcacggcg cccgtatact agccgccgtg cgccgagccc
      841 tcaccccggc ggcccgagtc caggagcccg catgcgacgt ctgacacggc cggccgcact
      901 gatcgcggcc ggcgtgctgg cgctggtgag cacgacatcc ggagcaccga tccgagcggc
      961 cgaagccccc ctgcgcttcc tcgccggacc ggccgggacg ctggatccgg ccttcatctc
     1021 ggacgccgcg gacgtccagc ttctcctcca gctgtacgcc ggcctgacgc gcctcgacga
     1081 gcagggtgag ccctacccgt cgctggcccg cagctgggag atcagcgacg acgggctcac
     1141 gtacgacttc accatccggg acgacctcgc cttcagcgac ggcacgcccc tgacggcgca
     1201 ggacgtccgt cgctcctggc tgcgcctgct ggatcccacc acggccgcga ccgccccgga
     1261 tgtcctgtct gtcatcagcg gcgcgcccga gcgcctggcc ggtgacgcgg atgaggacgt
     1321 ggtcgggatc caggtgaccg acgacacgca tctgcgcgtg acgctccgct acccggcgag
     1381 ctacttcctc gacatcctgg cgacgcccgc cgcgttcgtc gtgccgcctc gggccgacgc
     1441 cacgggcacg tggcagaccc cggacgactt cgtgggcagc ggcccgtacg cggtggacgg
     1501 gatcgacggc gtggacctgg tcctgcgcgc caacgacgag tacgtggccg gcccgccgcc
     1561 catccccgag attcgctggg tcgccgccgt cgacgccgac gctgccaccg ccttccaggc
     1621 cggtgacgtg gacctcgccc aggtcgccgg ctgggacgcc acctggatcg cctacgaccg
     1681 cgacctcggg ccccgcctgc acgccgcgga gccgctcacc atcagctact tcggcttcga
     1741 caccacctcc ccgccgttcg atgacgcccg ggtacgccga gcgttcgccc tggcgctcga
     1801 ccgggagcgg ctggtgcccc tgtcggaggg cgccgagtcg caggcggccg cgagcctggt
     1861 gccgccggcc atctggccgg acggtttcgc gccggggatc gaccatgacc tggacgaggc
     1921 ccggcgcctg ctcgacgagg ccgggtacga ggaccgatcc gatctcggca ccatcgtggt
     1981 caacggcagc ggcctcgaca tcggtcctgc cgccgccgcc tggcgggagg agctcggcgt
     2041 cgacgtgcgg gccgaggtga tggacttcgg ggacttcttg gcctcgctcg agaccgatcc
     2101 gccgaacctg ttcaccgtca actggatcgc cgactacgcc tcaccgcacg cgctctacgg
     2161 cctgctcctc gcccccgatg ccgcgagcaa ctggggacgc tgggacgacg acgagttcga
     2221 gtcgctgctg gaggcggcgg ccgcggctcc cgaggacgag gtgggccggg cctacgacga
     2281 ggtcgagtcc tacgtccagg agcaggcccc ggtcatcccg tggtcgtacg gcgagacatg
     2341 gtggctcgtg gccgacggac tgcgcgggct gggcaacctc accatcggcc tgaccgactt
     2401 cggacgcgtc tcgtgggacc gatgatcctg atcagccgct ggctggccgc accggcgctc
     2461 gccgtgacgc tcgcgctcct cggcgccggc agcgtggccg cgttcgacgg cttcggcacg
     2521 tcgacggcgg acagcacgta cgaccagggc atgaccttca ccatcgagct gacgggcgcc
     2581 gctccggatc ggctggagct gctgctgacc acgccggggt ccgacgcggt cttcgtcgcc
     2641 ccggtcccgg tgcgtggggc gtccgcgacc tacacctggg acaccgccgt cgaccacgtc
     2701 acgcccaaca ccccggtgac ctaccagtgg cgcgccatcg agggcgacga ggtgacgctg
     2761 agcgacccag cgacgctgcg gtacgaggac gaccgtcccg gactggactg gcagtcggcc
     2821 aacctgggcg atgccacggt gcactggtac ggcgacgcgg agacccaggc tcgacgcttc
     2881 ggtgagctca ccgctgtcgg cgtgcagcgc gccgaggacc tcctgggcac cacgctggcc
     2941 ggccccgtcg acgtcttcgt ctaccagtcg cgcgacgact tcttcggtgc gctcggaccg
     3001 ggggcgcggg agtggaccgg cgcggcggcc ttcaccgaga tccgcacgat cttcatgtgg
     3061 aactcgaaca gcgcgggtga ccaggcgtac ctcgagcagg cgatgatcca cgaggtcacg
     3121 cacgtcgtct tcgccgatgc caccgacaac ccgtaccacg agccggcgcg ctggctgaac
     3181 gaggggatcg ccacctggtc ggaggacttc gacggcgccg acgaggcggc catcgtgcgg
     3241 gtcgaggcgt ccggtggcgg gctcttcgcc ttcgaggcca tcgaggcgca gttcccgatc
     3301 ggggaccgcg gtggccgcct ctcgtacgcg cagggcacgg cgatgatcga ccgcatcgtg
     3361 gacacgtacg gtgaggagtc gctggcgaag atcgccgccg cctaccgcga cggagcctcc
     3421 gacgccgagg cgctcgaggc ggggaccggc gtgccggcgg agcagctcta cgacgacttc
     3481 tacgccgcct acggcgtgga tgcccccgag ccgatcgcgc cggacccgat cccggcatcg
     3541 aacgtcgacc ggcccgaggc gggcccgctg gatccgggcg gggtcgacgt gccggcgacg
     3601 ccgccgccgg gggacggcgc acccggcgag cacgacggat cggcaggcgg tgatccgggg
     3661 gcgccgctgg tcgtgggggc gctggtggtg ggcgccgcgg cggccctggc cggcgcgctg
     3721 tggctgtcgc gtcgcgcggc gcggagggcg gcgtgagccg ccggctgcca ccgaagcggc
     3781 ggttcgtgcg gccgcagtgg gcgatcagca tcggcgtggc gctcgcggcc atcggcttcg
     3841 tgggtgccgc ccagtggaac agctcgctgg cgcgccagga gttcgtcacc tcggcgcagc
     3901 gcgtcctcat cggagaggcc caggacctcc agcgggagca ggacgatctg cgtgcccaga
     3961 tcgaggaggc cgaggaccgc gtccgctcct tccagacgac gtcggccggt tcacaggccc
     4021 agctggacca gatcaacagc gacctggccg aggcgcgcct cgagagcggg ctggtcgccg
     4081 tccgcggccc tggcgccgtg gtcgagatcg ccgactccac gcgacaggtg ccgcccggtg
     4141 aatcggtcaa caactacctg gtgctggctg acgacctgcg cgacatcgtg accgccctgt
     4201 gggcatcggg cgcggaggcg atcacgatca gcggcgagcg gctcgtctcg acctcctcga
     4261 tctacagcgc cggctccgcc atcctggtca acaccgtgcc gctggtgccg ccgtaccgca
     4321 tcgaggcggt tggtcccgag ggcctccacg accggttcct gtcgaaccag gccttcgtcg
     4381 ggcgcgtcgg ccggcgcatc gagtcgtacg ggctccagtt cgccagcgag ccgcgtgacg
     4441 acctggagct cccggccttc gtcggcaaca cccggatgcg ctggggcgcg ccggagacgg
     4501 cggcggactg atggccggac ggatcgccca gctcagcctg ttcgtggtcg ccgggctcat
     4561 cggcgtcctg ttggtcgggc agctgcgctc gcaggcacgg ccgatcgagc tgtccaacct
     4621 ctcaccccag gagctgtccg gcctggtgga gacgctccag cagcgcaacg tggagctcac
     4681 caacgccctc gccgagctgc gcgagcaggt gcgggactac gagcgagcgg aggtgcaggg
     4741 gcagtccacc ctggagctga ccagcgagga gctgacgcgc gtgcgggcct tcgcgggcct
     4801 cgagccggtg gtcgggcagg gcatcgccat ccgcatgacc gggcccttcg acccgaccgc
     4861 cgtcaacgac ctcgtgcacg agctgcgcaa cgcgggagcc gaggcgatcg ccatcgacgg
     4921 gatccgcatc acgggccgct cggtggccgt gctgggcacc agcgccatcg agatcgacgg
     4981 gcgtccgctg gacgagacgg tcgaagtgct ggccgtgggc tacccgaccg ggctgcagtc
     5041 atccatcgag cggcccggcg gtctcaagac gtacctggag cagtcactcg acgtcacgat
     5101 gtcgatcgag acgcgccgga acctgcggct gcccgcgacg gagcgcgatc tcgctccgac
     5161 ggtggccgag ccggtcgagt agccttgtcg ccccgggcgg aactcgccgt acgaaggatg
     5221 ccgtgaagga actcatcgac cgcgccctgg acgctgccga gcgaggaggc gcgagctaca
     5281 ccgaggtgcg gatcgccgag cgcgagaccg agcagctgac cgtgaagaac ggcgcgctgg
     5341 aggccgcgac ctccaatgcc agtgggggat tcggcgtgcg cgtcctggtc aacggggcgt
     5401 ggggcttcag ctccagccgg gtcaccgatg ccgaggaggg cgagcgcatc gcgcgcgagg
     5461 cgctcgagat cgcgcgcgca tcggcgctgg cgcagcgcgt gccggtggag ctggacgaca
     5521 cgccgccggc ggtcgacagc tatcgcacgc cgttcgagga ggaccccttc gcggtcagcc
     5581 tggacgagaa gctgcggatc ctcatggagg cggacgaggc gatggcccgc gttccgggcg
     5641 tcgccattcg cgagggcgtc atgttcgccg gacgagagcg caagacgttc gcgtcgagcg
     5701 ctggcgcgcg gatcgagcag gagatcgtcg aggccggcgc cgggatcgag gcgaccgcgg
     5761 tgaacgagtc cgagatgcag cagcgcagct acccgaactc gttcggcggc cagatggtga
     5821 caggcggctt cgaggcggtc cgcgccctcg acctggccgg caacgcgcag gagatggcgg
     5881 agctcgccgt tgccctgctc gacgctccgc agtgcccgtc cggcgagatg gacgtgatcc
     5941 tcgactcgac gcaggtggcg ctccagatcc acgagtcatg cggccacccg accgagctcg
     6001 accgcgtgtt cgggacggag gcctcgtttg ccgggaccag cttcctcacg accgacaagc
     6061 tcgggcagct gcggtacggc agcgagctcg tcaacatcga cgccgatgcg accgcgcccg
     6121 gggggctggg gacgttcggc tatgacgacg agggcgtgcc cgctcagaag gtcccgctca
     6181 tcgaccacgg gctgctggtc ggatacctga cgagccgcga gacggcgcca cgcatcggtc
     6241 ggcgcagcat gggcagcgcg cgggcctgga cctggagcca tatcccgctg atccgcatga
     6301 cgaacatcaa cctgcgaccc ggcgacgccg gctcgctcga ggacctcatc gcggatacgc
     6361 gtgacggcat cttcatgagc atcaacaaga gctggtccat cgacgatcgg cggctgaact
     6421 tccagttcgg cgaccaggcg ggatggctga tcaagaacgg gaagcgcgcg cagctggtga
     6481 agaacccgac ctacaccggg atcaccccgc agttctgggg cagctgcgac gccatctgct
     6541 ccgccgacga atggacgctg tgggggcttc ccaactgcgg caagggtgag ccgatgcaga
     6601 ccggccacgt cggccacggc gcggcgcccg cgcgcttccg caacgtgaag gtgggagtgg
     6661 gccgatggta gccacgcgcg acgaggtcag cgtcctgctg gaccgtgtgc tggagtacgc
     6721 cggtggcggc gtccaggcgg aggcgctgta cctcggtgag gacgccgcgc tgacgcgctt
     6781 cgcgaccaac cagatccacc agaacgtccg cgcgcacgac gcctcgctgc aagtccgcat
     6841 catcgacggg gagcgcgtcg gggtggcggc caccaatcgc ctggacgagg cctcgatccg
     6901 ggacgtggtg gagcgcgccc gcgccatcgc ccagcgctcg gcgccgaacc cgcgtaccgc
     6961 ggtcctgccc gagccgaccg accgggatca tgagagcgac ctcgggtacg tggcggagac
     7021 ggccgaggcc agcgccgacg tgcgcgccga gggcgccgcc gcggtcatcg ccgccggcga
     7081 ggcgaagaag ctcgagacgt cggggtcgta cgccacgtcg accatgacga tggcggtcgc
     7141 caacacccgg ggcatccgca gcctgcatcg gtcgacccgt gcggcactgc tgacggtgat
     7201 gatggacggc ttcgcgtcgg gcgcggcatc ggggtacgcc aacgccgtct caccggacgt
     7261 ccgggacatc gacgccgccg cgctcggggc cgaggcggcc gagaaggggg accggatgcg
     7321 cggtgccggc gagctggagc ccggcgagta cgaggtcatc ctcgaggagt acgcggtcgc
     7381 cgggctgctc gagtacctct ccttcatcgg cttctcgggc ctggcgcacg aggagggccg
     7441 ttccttcatg gagctcggca aacgggtgat gggcgagaac gtctcgatct gggacgacgg
     7501 ccacgatgtc accggcatgc cctcaccgat cgatttcgag ggcgtggtgc ggcagcgggt
     7561 cgacctcatc cgcgacggcg tcgccgcggc cgtcgtgcac gacgccgcca ccggcgcgcg
     7621 cgccggcacg ggctcgacgg gtcacgccct gccggcgccg aacacgttcg ggccgatggc
     7681 gatgaacctg ttcatggcgc cggggacgac gccgcgcgag gagctcatcg cgggcgtgaa
     7741 gcgtggggtg tgggtgacgc ggttccacta catcaacccg gtccatccca agaaggcgat
     7801 cctcaccggc atgaccaagg acggcacgtt cctgatcgag gacggccgca tctcgcgacc
     7861 gctgttgaac ttccgcttca cgcagtcgat tcccgaggcc ttcagcgacg tgcgtgccgc
     7921 ctcgcgcgag acgaagctgc tgcccgggga gttcgcgggg agcgcgcggt gtcccgccct
     7981 gcacctcggc tcgttcaact tcaccggtgt cacggccgcg gagggcgccg ggtgacggtg
     8041 gccgccccgc tggacctccg catcggcgat cgcctgcggc tgcgcaagga gcacccgtgc
     8101 ggaagccgcg agtgggacgt cgtgcggctg ggtgccgaca tcgggctcgt ctgcgccggc
     8161 tgcgggcatc ggatcctgat ggatcgcctc gacgtcgagc gccgcttcac cgcgttcgtc
     8221 cggcgcggcg acgagggtcc ggcctgaccg atggcccaca tcgaggttcc gactgacgcc
     8281 ggcgacgagc tggccggagc ggggaccgtc ttccgcaacc gcgccttcgt ctacctctgg
     8341 tcggcccagg cgctgagcca gctcgccagc aacatggtgc tcgccgcgct catcgccacg
     8401 gtcgtcacca cgaccggttc gctgactgcc aacgccgtcc tcatcctgac gttcctggtg
     8461 ccggccgtgc tgttcagcac gctcggcggc gtcctggtcg agcgcggcga cgcgaagatc
     8521 atcatgctcg ccacgaacgt ggttcgggcg gtcggcacgt tcctcttcat cctggtcgcg
     8581 ccgacgacca gcacggccat cgtcccgctg gtctacctga tcaacttcgg cgtggcgacg
     8641 gcgaccgcca tcttcgcccc cgccgaactg acctcgatcc cgcgcatcgt cgaccgccgg
     8701 cacctgatgg cggcgaactc gatcttcatc ctgaccgtca acgccacgtt tgccatcggc
     8761 ttcggggtgc tcgggccgtt ggtcctgaac gtcttcggcg cgacggcggt gtacgtcatc
     8821 gtgggctgca tgttcgccct cgccgccgcg gcgatcctgc cgctcccgtc cgtcaagccg
     8881 gaccaccggc cgtcggcggt gggcgacgct gccgggcgcg ccgtgcgcga gctggtggac
     8941 cagctggccg agggcgtccg cttcgtgcgc agccaccgcc ggatcgcatg gagcctcacc
     9001 tacctgggca tcgccgccag cctgatcggc gtgctcggcg ccatcggtcc cggcttcgcg
     9061 accgacatcc tgcgccttcg ccccgaggac ttcttcttca tcatgggtcc cgccgggctc
     9121 ggggcggtcg tcggcatcct cttcctcaac tcatacggga agggcattcc gaagcgcttc
     9181 gtgatcgaca tcggcttggt ggcgatgggc ttcacactcg tcgccctggc gctggtgcgg
     9241 ccgatcacga gcctggtggc gccggcggtc gcccccatcg aggacgtctt cccgaccatc
     9301 gcgccgatca ccagcctcat cgccctggtg gtggtcatcg cggtcatggc cggcgtggag
     9361 tacgccttcg tggccattcc gtcccagacc gcgctccagg aggagctgcc cagcgacgtt
     9421 cgtggccgca tcttcggcat cctgaacacg ctgctgtcgg tcgcctcgtt cctgcccgtc
     9481 atcctggcgc cggcggctgc cgacctcatc aacatcttcc tgcggggggc cggcatcccg
     9541 atcgtcatgg ccatcctcgg ggcgctcacg ctgtgggccg gcatcgcgtc ctggcggcgc
     9601 aacgcgaggg ccggcctgca ccagcacgag acgggccagt cgccggccga gggagtcgac
     9661 agcgccccgg ctccgggtac gcccgagcgg ccggcggtcg atccgcccga ggcaccccac
     9721 cgacaccgcc gacgccgtcg gcagccgccc gatgcgggct agggccatct cggccgtctt
     9781 cctgctcgtc gccgccgccg tcgcctggct caccgtcacc gaaccactcg ccgctcgcgt
     9841 cctccaggct ggtgtcgccg cgtcgctggt ctacgtcgcc tggctcgcgt ggcgtggatc
     9901 gcgcctgatg cgggcggcgt tccgtgcggc gcgtcacccg acgggggagc ggatggatgc
     9961 cggaggccag ctgccgcccg tgacgctggt cgtcccggcc tggaacgagg cgtcggtcat
    10021 cgccgagacc gtcgcacacc tcgcgtcctt cgcctacgcc ggcgaggacg gccgcccccg
    10081 ctacgacgtg ctggtggtcg acgacggctc gaccgacgac acgggcgcgc tggcgcggtc
    10141 ggcggcgtcg agcgcgccgc acgtccaggt gatgcgccgg gagccacagc gcgacgcgcc
    10201 ccgcacgaag ggcgcggtcc tggcatgggc gatggacggc gtccagggcg aggtggtcgg
    10261 cgtcgtcgac gcggacacgc gggtcggtcc gtccttcctc caggacgcga tgctcgcctg
    10321 gcggggggat ccgaccgccg ccgccctcca ggtcgccagg cgcccccaca atgcgcatcg
    10381 atcgtggctg accgcggccc aggccgagga gcagctgatg gacctcgcca gccagtgcgg
    10441 ccgccgcgcg gtcgacggga cggccgagct gcgtggcaac ggcatgttcg ttcgcgtcgc
    10501 cgcgctcgag gcggtcggcg gctgggacgc gaccgcgctc acggaggacc tcgagctctc
    10561 gacacgactc gccattgccg gagagcacgt cgccctggcg ccgcacgtcg ccatcggcga
    10621 ggaggcgatc gagtcgattc caccgctctg gcaccagcgg gtgcgctggg ccgagggaag
    10681 cctgcggcgc ctcatccagc acgcgcccgc gatgctctcg ggctcgcagc cgctcagccg
    10741 caaggccgat ttccttgcct tcgccgccga gttcctgctg ccgccgctgt tcgcgacgac
    10801 caccctggcg agcctcgtca ccgtcgtgat gccgcagccg gcggactgga ccgtgcccgt
    10861 ctcgcttgcg ctcggttacg gcctgggcgt gttcctgctg gcgctcggcg gcctctcggc
    10921 cgtcggggtc cggggcctct ccctggtcgg acgtgccggc cgaggatcgc tcttcctctc
    10981 gcactggctg ctggtggtgc cgtgggcgct ggtccgcatc ctggcgggtc caggggaggg
    11041 ccacttccga tccacgccgc gcttcacgcc caccacgccg tgaccgcctc ccacgcaacg
    11101 atcgtccgcg gggccgtcat cctgcgcgcc gagggcggca tgctgcgcac cgcggaggcc
    11161 atcgcgatcc gcgaccggcg cgtcgtcgcc gccggctccc gtgccgaggt ggaggcggcg
    11221 gtgccggtcg cgggcacggt ccacgacgtc ggcccccgcg tcgtcctgcc ggggatccac
    11281 gatttccacg tgcacctcgt cgggctggcg cgcgctcgcg ccggggtcga cctgggcgcg
    11341 gcgacccgtc cggcaggcct cctggaccgg ctgcgggaag ccgcgcgcgc aacccctcca
    11401 ggcgcctggg tgaccggccg gggctggacc gagacgcagg tcgtcgcggc cggtctcgac
    11461 cgcatggccg atgcggtcgg gtcgcgggcc ggcttcctcg cgagccacga tggccactcc
    11521 gcgtgggcgt cgcccgcggc gctgtccgcc gcgcggatcg ggccggagac gcccgatcca
    11581 ccggggggtc gcatcgagcg tggggcccat ggcgtcccga ccggcgtcct gcgcgagacg
    11641 gcgctcgacc tggtcgcgtc gcacgtgccc aagctccagg gtgaagccct gcgcccgcac
    11701 ctcgaacgcg tgctgcggga gctcgcgtcg ctgggcatca ccggggcctc ggaggcgggg
    11761 gactacacga acgagggcgg catcgggcgc gacgcggcga tgggggactc gttctcgacg
    11821 ctcacggatc tcggcgggct ggtcgacggc cgcctccggc tctcgatcgg cattcccgtc
    11881 gacgcgcttc ccgcggcggc cgcgcggggc ttcacgaccg gcgcgccgct gggcgagtcc
    11941 aggacccttc gcttcggctg ggcaaaggag tacgccgacg gggcgctggg atccggtacc
    12001 gccgcgctct tcagcccgcg aacgtgcgga tcgggcggca ccgggatcat gcgcgtcacc
    12061 gacgacgagc tcgacgctct cttcgcgctg gggcgaagcg cccgcatcgg tctcgcgatc
    12121 cacgccatcg gcgatcgagc ggtggccgcc gtcatcgacg ccgccacgcg agcgggcccg
    12181 ccggccaccg gggtgccgtc ggatcggatg gagcacgtcc agctgtcgcg gcagcaggac
    12241 cggccgcggc ttgccggccg gttgacggcc agcgtgcagc cgatccacgc cgccgcggac
    12301 cgggacctcg tggaggcgtg ctgggacggc cggcaggacg acgcgtacgc ctggcgctcg
    12361 atcgtggagg ccggcgcgcg gctcgcgttc gggtcggata cccccgtcga gtcggtgaac
    12421 ccatggctcg gcctccacgc cgcggtccac cgacgcctac cgggggacga gcgcgatgac
    12481 tggcgtccgg cggaggcgct cacggcggtc gaggccatcg cggcctacac caccgggccg
    12541 gccctcgcac tcggccgcga ggacgagggt catctggaac ccggggctcg ggccgacctg
    12601 gccgtcctcg acgtggatct cccgaccctg ctcgccgccg acgaacggct gggaacggtg
    12661 acgtccgtgc tcaccatggt cgacggggac gtgatccacg agggctgatc ggccgttgcg
    12721 cgggcgcatc gcccggcgct aggatgctgg ccgggcaagc ggcctccccc gcggcgcatg
    12781 cgacacgccc gccggaccgc ggctcccatc ggaggagagc agatggatcg ctcacaggat
    12841 gagccggagg gggcgtcggg acgtgtgccg gtcggacccg ggccagcgag cagcttcgat
    12901 ccgacggtgc tcgacttcgg gcactacgcg ggccgcagcc tcgaggagct ggcttcggtc
    12961 gatccggagt acctgcgctg gctggcgcgc cacccgtccg ggtaccggta ccggaccgag
    13021 atcggccgcg tcctggcgtc ggcggtgcct cgcgccggcg actgggagcg ctgagcgcgg
    13081 ggccgttcga cccggcgccc ccgggtcgcc cggctctggg gatctgaccg ccgccatggg
    13141 atacctgtgg catgacctca acgcgtcccc gcccgctgtc ggcggcagcg ccacgccgcg
    13201 ccggcggggc aaccggtccc tggcgccaca tcctgctcgc ctgtgacggg gctgccggca
    13261 ccatcgcggc tgggcgccgt gccgtcgagc tcgcggcgcg cgacggcgcc gcgctgacca
    13321 tcgtcctggt gcgcccggca ccggcgaccc gcgcggagcc ttcggttcca ccggccgccg
    13381 acgacatcgc cagtctgccg gaggcgatcg aacgcgtcct cgccgccgcc acacgcgcgg
    13441 gcatcgccgc acacggggag gtgcgccgcg gacagtcggg ggcggcgatc ctggacgtcg
    13501 cccgcgaggt ggacgccgac ctgatcatca tcgggcgcca cggcccgtgc atcgggacgt
    13561 ccttcgcggt ggcgaactgc ggttacgtcg tgacgcacag cgaccggcca gtgctcgtgg
    13621 tgcagccgtg ggcccgagaa ggagacctgc tcgacgcccc gcttcgaccc gcagcggagg
    13681 aggcctccta gcccgtagca ttcgaggaca gccgcatccg atgtggggga tggacgtttc
    13741 aacgccacgg cgtctcgcca gccctgccgc gcgcgggctc accgctctcg gtcgccgggc
    13801 cggaatcgcg gtgcgcgcgc acgaagcgaa cgtcgtcgtg gcggccggat cgctcctgct
    13861 ggtcctcgtc tcgatgctcc gcgagccggc cgttccgtgg ctgacgtggg acagccgggt
    13921 ctactgggag acgagcctcg cgcccgacct gtacgccaac gcacgggtcg gtgccctcgg
    13981 cgcgtacctg tactcacccg cgttcgccca gctcctcgct ccggccggcc tcctgccctg
    14041 gccgctgttc ctcttcggct ggacgttcgt ctcgatccgg gtggccgcct ggctctgctc
    14101 cggaccgatc cgccgccacc gggcgctgtg gctgccgctc gcgacgctcg cgatcctgga
    14161 catcggggcg gggaacgtga acgtgctgat cggtggcgcc atcgtgctcg cgttgcgctg
    14221 gtcgggggcg tggagcgccg tgctgctcac caaggtgacg ccggggatgg ccgtcatctg
    14281 gcacctcgtg cggcgcgagt ggcgcccgct gctcagcgcg ctcgcggtga cgctcctggt
    14341 cgtgggcatc tcggcggtgt tcgaagccag cgcctgggtc gactggggca ccgtgctgct
    14401 gtcgaacgcc acgccgcatg agctgagtgg cgacctcgcc atcccggccg ccgtgcgctt
    14461 cccggtcggg ctggtcatcg tcgcgtgggg agcgctgacc gaccgacgct gggtggtccc
    14521 gatcgcggcc atcctcttcc tgcccgtgat ctggcccaac acgctggccc tgctgctggc
    14581 gtcggccgcc ctctgggccg atgcgcagcc ctcgccggcc ggcggcgctg ccgtgcgccg
    14641 aacggtaccg gcatgaacgc acagcgcctc gtgtgggtcg actggctgaa ggggctcgtg
    14701 gtgctcggcg tcgtggcgtt ccacgcggcg cagccgtacg tcgtcacgga ttggatcgtc
    14761 aacgcgaccg aggacagcta cgtgctgagc ggcctggcag ggctggggta cctgttcgcc
    14821 atgccgctga tgttcctcct cgctggcgtc actggctgga tcgcgctgga gcgccgcacg
    14881 acctggcagt acctcagcgt gcggctcctg cgactcggcc tcccgctcgt ggtcggcctc
    14941 gccttcctga gccctctcga gagctgggtg gggcagctga gctacggaga gactcccgat
    15001 ctgccgtcgt tcgtcgcacg cacgtggtcg gagctgcccc cgctcacgtc cccgcgctgg
    15061 ctgggtgacg tgggccacca tctgtggttc ctggggttcc tgctcggcta cgtgctgctg
    15121 accatcgtgc cgctgcgctg gttgcggcgt cgcaccccgc cctccgccac gcgccacgtg
    15181 ccggccgtcg tcgcgatgct cgggccgatc gtgccgatcg cgatcctgca gtggctggtc
    15241 cgcccgccgg caccgcagta ccgagactgg gccgatgccg cggtgtggct cgtgtacttc
    15301 tgcatcggga tcgggctggc cttcgaccgg gcctggctcc ggccgatcgc ccggagcggg
    15361 ggatggatga ccatcccggc actgctgctc gccgcgctgc tgcttcccct gggcctcgac
    15421 ggcgtcatgc gcctcgagtc ggcgccctcg ctcggcgttg cctcccttgc gtacgcctcc
    15481 gcccgcacgg ccatcggttg gtggctggtg tgcgcggtga tggcgctcgg tatgcgctgg
    15541 ctgacggggg ctgcctgggc ggcggagcgg gcgggcgagg cgtcgctcgc gttctacgtc
    15601 ctgcaccatc cgatcgtggt ggtcatcgcg gcggccgtcg ccccgtggtc ggttggcgcg
    15661 ctcgcgggct tcgtggtgat cttcgtgtcg tcggtcgcgg tcagcgcggc gctgtacgag
    15721 ggcgtcgtcc gccgggtcgg cgtcctgcgg tccgtgttcg gtgtcgccga gcctccgccc
    15781 gagcggcccc tgggtccacc ccagcctgag ccgatctgag ccgccccgct ccctgaccgc
    15841 ccccgtcacg gctggattcg tgatcgtcgg catgaggggc gacgctcacg gccctgctcg
    15901 ccagcgaggt gacgacggcc tcggacgggt agcgcatacg aggcagcgga gggcctcagg
    15961 cgggcggtgg gggaccgggc gggactcgcg agctgatgct gtcatcggtg ccgggcaggt
    16021 ccgagtcgac gtcgctcgga taccgccgct gggccgcggt cagggcgcga agaatttcgg
    16081 atcgaccggc gtcgaggatg acccgggctt ccatggcggc cgccacattg ccgcgtggct
    16141 ttgtgagctc gctggcggcg gcgcggctgg cacgctggta cgcctggatc gcgtcgttgg
    16201 ccacgcgggt gatgcggttc gtcgtctcgc gaagcagccg caggcgctcg gccggccgcc
    16261 gttcatgctg caggtgccga tcgaggtcgg cgccgaggtc gtcgatgagc gcggcggcgc
    16321 cctggaccgt ggtgatgtcg cgcgcccgca ggctcagccc ggtccagcgt ctcgcagtgg
    16381 gtagcacagc acacctccat cctggatgcc ccgggcgatc ggcccggagg ccatcgtgcg
    16441 cgacctggca cgacggagcg tcggtcgcgc ccgaaccggg aaggtccgga cgcgatcggc
    16501 gtagggaggt caggcgccct gcacctgcac cgcgcgggga cccttcgggc cagcctcgac
    16561 ctcgaatgtc accgactcgc cgccaacgag gcgatcgaag tccagggacg aggtgaggcc
    16621 gtccttgtgg aagaagtatt ccttcccgtc gggggcggcg atgaagccga agccgcgctc
    16681 tgagatgacg cgcttgacgg taccggtggt catgagatga agcccttctc tcctcgaact
    16741 gtcgtgcggc gctcggccgg ttcgagaaga acctcatcga ccaggcgaag actgccgaga
    16801 tgcggcagga ccgc
//
LOCUS       DASTYP010000101         9017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_989890, whole genome shotgun sequence.
ACCESSION   DASTYP010000101 DASTYP010000000
VERSION     DASTYP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989890"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFV59_04930"
     CDS             complement(<1..795)
                     /locus_tag="VFV59_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5325208.1"
                     /translation="MTSPHRGPSPDLVGAGLVALAASCFGTLGPLSRFAGDAGVDALT
                     LVTWRAAIGAMVVTAFIAAWAIGRGIRPASLRQIPVRDRWFMLAAAGANMVLNLAAFV
                     AFERISIALTLLVFYLYPAGVALVSTMWFGERLDRLRWGALVVSLAGMALVVAGAESL
                     GSVDPVGIGLAFLAAMAQVFYVLAARHGFAHVPGPQAAALTMGGAATLYVLVAFGIGT
                     VGALGQPVASSAAFVPVLLAGVIGAGIPTLAFILGIRRLGPSQAAIL"
     gene            complement(792..2798)
                     /locus_tag="VFV59_04935"
     CDS             complement(792..2798)
                     /locus_tag="VFV59_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU5325209.1"
                     /translation="MSLVTLTGVAKSHGPQHLFSDVGLQISAGRRLAIVGPNGAGKTT
                     LLEIITGDQEADAGTVTRARDVVIGYLRQEVAESRGRTALAEVLAGAGEVSGIERRMR
                     HLEMELEDAADDDELAELMDEYGRLQHRFEAMGGYGLEAEARRILAGLGFGESDMERD
                     IGEFSGGWMMRIALARLLLQNPDVLLLDEPTNHLDLASVEWLQGFLSQYAGAIVLVSH
                     DRDFINEIVNRVVELHDGRATEYVGDYADFVEQRTERMAQLEAAAKNQQRKIAHTEAF
                     IERFRYKASKARQVQSRIKALERMDRVESPGRRTRSVKFRFPEPPRSGRTVITLADIA
                     KSYGSTVVYRGDLDLTLERGQKVALIGPNGAGKSTLLKILAGVLPFEGGTRELGSNVR
                     VAYFAQHQIDALDPTKSVFEELNDAAPRLSTAEVRKLLGAFLFSGDAVEKKVGVLSGG
                     EQTRLALAKLLADPANLLCLDEPTNHLDIQSRDVLEDALNAFSGTIVLITHDRYLIRS
                     VANTIVEVNAGEATVYPGDFEYYAAKRGVDIETRGAVEGAHASPRGVVVDASKPRESA
                     REAAERKRREAEERNARHRRTRDLRESLRRAEHEAREADAELTALTERLADPSIYADA
                     DLVRSLVERHNALRDRTDALASERERLSAELSVAEAEPVGGGRR"
     gene            complement(2946..3860)
                     /gene="thrB"
                     /locus_tag="VFV59_04940"
     CDS             complement(2946..3860)
                     /gene="thrB"
                     /locus_tag="VFV59_04940"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU5325210.1"
                     /translation="MPPIASVDVPATSANLGSGFDCFAGALSLKLRAELWHGDEPGIL
                     LNARGEGAPLPGEPADENLLIRGFREGLRVSAAPGAGSWRIEVSSMIPAARGLGSSAA
                     AIVAGVVLGAAVGRRQVEADELLELASRLEGHPDNVAAALYGGFTIAVADPGQPITLR
                     RFRVPEAWIPVAFIPQRESRTHEMRAALPSSVSHAAAARQAGRTALLATAIMTSDAGL
                     LRVAMADELHQPYRLPVLPGTRDLIGLAYERGAAGACLSGAGPTVLAICDSVAGAHAV
                     EKAWNGAGLPGMATRLRFDTAGARLIAD"
     gene            3982..4482
                     /locus_tag="VFV59_04945"
     CDS             3982..4482
                     /locus_tag="VFV59_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5325211.1"
                     /translation="MSRTAQPTTRRATRRRAEAAPHQPMTAEAALRDDPVVWLSSVQP
                     DGRPHVVPVWFHWDGERIVAFSKPRARKIENLREHASVMLAVGTPGPDFEVELIEATA
                     ELPDVPASDVIPDGFGAKYRELLRRAGLTVQKFAEVYSQPIVLRPTRFLGYGGRGWDR
                     AQAVAS"
     gene            complement(4492..4860)
                     /locus_tag="VFV59_04950"
     CDS             complement(4492..4860)
                     /locus_tag="VFV59_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5325212.1"
                     /translation="MPTPDLSVLVVDDEPDIRATVSAMLEIEGYRVTEATNGADALAV
                     VQREEPDLILLDMRMPVLDGWGFADELRRRGVRIPIVVMTAARDAATWAAEIAASDYL
                     AKPFGFDDLISAIERVQTSP"
     gene            complement(4847..6778)
                     /locus_tag="VFV59_04955"
     CDS             complement(4847..6778)
                     /locus_tag="VFV59_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU5325213.1"
                     /translation="MTALELIGLVNQVLFIGLFVAVLVAAVRQPTRTRLDALLLFGSA
                     ASVVILGRLADWLGFSGQPWVGSVTLLLLNVAPLAMLRLVDDFSDSPRWVQMAGLVAF
                     VAIGILGFILPVTNETVLIATMAFLVVVGGYAAVAFARQSQRTRGITRRRMTAVTVGA
                     GAFIAALLVAFVGALDPDLAQTTRPVTQLLALVAVSAFFLGFTPPRWVRLVWRDPDVR
                     RFLERSTQIIGVTDDYAALTQMQAAAADAFGANGAVIGVAVPERGTLRYAARSGWEEY
                     PDDLYVGGRAFTDRRRVVVLDSAAFDPENVEAYRRVGARTVIASPIVTDGEPLGVLTI
                     FASRAPIFAEDDLWLLDLLASHTGVLLEARALSAQASDLRAREDAARLKEEFISAAAH
                     DLRTPLTVVLGQAELLQRRILRRPNEPPDPASIGRIVREAHRLRDLVTELLDAQRLEE
                     SRAVLRFQRVDLRQVVQTVVERHREYGATLEADLAALELSVEADTGRMEQVVENLVEN
                     ALKYGGDAPPSIRAWAADEQALVSVVDHGIGIPEPERERVFERFFRASNAHSITDTGI
                     GLGLYICRRIVEAHGGGIWFEPTPGGGSTFTLSVPIARRNAEAHGMTVDPAADAAPPM
                     DAAWNPTPALSTDSAHADA"
     gene            complement(6870..7442)
                     /locus_tag="VFV59_04960"
     CDS             complement(6870..7442)
                     /locus_tag="VFV59_04960"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5325214.1"
                     /translation="MTLDETTARCDWATGDPLMTDYHDAEWGVPLRDPHRLFELLCLE
                     GAQAGLAWITILRKRDGYRAAFDGFDPHRIAGYDDADRERLMTDARIVRNRAKIDAFI
                     GNARAFLAIPDFVDLVWSVVDGRTVVNRWTLMEQIPAQTGASDALSRLLRDRGFRFVG
                     PTICYAFMQSAGLVDDHLVTCFRHGLAAKS"
     gene            complement(7559..7738)
                     /locus_tag="VFV59_04965"
     CDS             complement(7559..7738)
                     /locus_tag="VFV59_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325215.1"
                     /translation="MNPTAALLLARATEDDYRRQLRQRQLAKAARREDRTESRSWLDR
                     IRSARPRLADSASRA"
     gene            8098..>9017
                     /locus_tag="VFV59_04970"
     CDS             8098..>9017
                     /locus_tag="VFV59_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5325216.1"
                     /translation="MAPELAPQHLSFLFADVEGSTRMTEQYGVVAGAALTRYHEIVAE
                     RAAAHGGRIFERIGDGAYACFDAAREAVQAADDIQGVMTDTDWGEIGRVRVRLVVVSG
                     EVEVRGDRFFGLPLYRAARLQALADGGETLLAGTTVDELGGWLPDGTMLRDLGAHRLR
                     DMVEPERIFALVHTSRARLGDEPPPEPSRIQGTETVTDEEGPIRVLLVDDHAVVRRGL
                     RGFLELLKDFDVVGEAENGREGVAAADRLVPDVILMDLLMPEMGGLEAIAAIKQAHPE
                     IEIVAVTSFIEEEKVTSALEAGASGYLLKDA"
BASE COUNT         1301 a         3162 c         3313 g         1241 t
ORIGIN      
        1 gaggatggcc gcctgcgagg gaccaaggcg ccggatgccc aggatgaagg cgagggtcgg
       61 gatgccggcc ccgatgacgc cggccagcag gacgggaacg aacgccgcgc tcgacgcgac
      121 cggctgcccc agcgcgccga cggttccgat cccgaacgcg accaggacgt agagggtcgc
      181 cgcgcctccc atggtcaggg ctgcggcctg cggcccgggg acgtgggcga agccgtggcg
      241 cgccgccagg acgtagaaga cctgggccat ggcggccagg aacgccaggc cgatgccgac
      301 cgggtccacc gaccccagcg actccgcgcc cgcgacgacg agtgccatgc cggccagcga
      361 gaccaccagg gcgccccagc gcagccggtc cagccgctcg ccgaaccaca tcgtcgagac
      421 gagcgccacg ccggcggggt agaggtagaa gacgagcagg gtcagggcga tcgagatgcg
      481 ctcgaaggcg acgaacgccg cgaggttcag gaccatgttg gccccggccg ccgcgagcat
      541 gaaccagcgg tcgcgcaccg ggatctggcg cagtgatgcc gggcggatgc cgcgaccgat
      601 ggcccaggcg gcgatgaagg cggtcaccac catcgcgccg atggccgcgc gccaggtgac
      661 cagggtcagg gcgtccacgc cggcgtcgcc ggcgaagcgg ctcagcggtc ccagcgtgcc
      721 gaagcaggac gcggccagcg cgacgaggcc ggccccgacg agatcggggg acggaccgcg
      781 gtgcggcgat gtcatcggcg gccgccgccc accggttcgg cctcggcgac gctcagctcc
      841 gccgacaggc gctcgcgctc gctggcgagc gcatcggtac ggtcgcgcag cgcgttgtga
      901 cgctcgacca ggctgcgcac gaggtcggca tcggcgtaga tggacgggtc ggcgaggcgc
      961 tccgtcagcg ccgtcagctc agcgtcggcc tcgcgggcct cgtgctctgc ccgacgcagc
     1021 gactcccgaa ggtcgcgtgt gcgtcggtgc cgcgcgttgc gctcctccgc ctcgcgacgc
     1081 tttcgttcgg ccgcctcgcg cgctgattcg cgtggcttcg aggcgtccac caccacgccg
     1141 cgcggggagg cgtgcgcgcc ctcgacggcc ccccgcgtct cgatgtcgac cccgcgcttg
     1201 gccgcgtagt actcgaagtc gcccggatag acggtcgcct cgccggcgtt cacctcgacg
     1261 atcgtgttcg ccacgctgcg gatcaggtag cggtcgtggg tgatcagcac gatggtgccg
     1321 ctgaacgcgt tcaacgcgtc ctccagcacg tcgcgcgact ggatgtccag gtggttcgtc
     1381 ggctcgtcca ggcacagcag gttggccggg tcggccagca gcttcgccag ggccagccgg
     1441 gtctgctcac cgcccgacag gacgccgacc ttcttctcga cggcgtcgcc gctgaacagg
     1501 aacgcgccga gcagcttccg cacctcggcc gtcgagaggc ggggcgccgc gtcgttcagc
     1561 tcctcgaaga ccgacttggt cggatccagc gcgtcgatct ggtgctgcgc gaagtacgcg
     1621 acccgcacgt tggagccgag ctcccgcgtg ccgccctcga acggcagcac cccggcgagg
     1681 atcttgagca gcgtcgactt gccggcgccg ttcggaccga tgagcgcgac cttctggccg
     1741 cgctcgaggg tcaggtccag gtcgccgcgg tagaccacgg tgctgccgta cgacttggcg
     1801 atgtcggcca gggtgatcac ggtgcgcccg ctgcgcggtg gctcggggaa gcggaacttc
     1861 acgctgcgcg tccttcggcc cggcgactcg acccggtcca tccgctcgag cgccttgatc
     1921 cgcgactgga cctggcgggc cttgctcgcc ttgtatcgga accgctcgat gaaggcctcg
     1981 gtgtgcgcga tcttgcgctg ctggttcttc gccgccgcct ccagctgcgc catccgctcc
     2041 gtgcgctgct cgacgaagtc ggcgtagtcg cccacgtact cggtggcgcg gccgtcgtgc
     2101 agctccacca cgcggttgac gatctcgttg atgaagtcgc ggtcgtggct gaccagcacg
     2161 atcgcgccgg cgtactgcga caggaacccc tgcagccact cgaccgacgc gaggtccagg
     2221 tggttcgtcg gctcgtccag cagcagcacg tccgggttct ggagcaggag ccgggcgagc
     2281 gcgatgcgca tcatccagcc accgctgaac tcgccgatgt cgcgctccat gtcggactcg
     2341 ccgaagccca gcccggccag gatgcgccgc gcctccgcct ccagcccgta gccgcccatc
     2401 gcctcgaacc gatgctgcag ccggccgtac tcgtccatca gctcggccag ctcgtcgtcg
     2461 tcggcagcgt cctcgagctc catctcgagg tgccgcatgc gtcgctcgat gccgctcacc
     2521 tcgccggcgc ccgccagcac ctcggcgagg gcagtccgac cgcggctctc ggcgacctcc
     2581 tggcgcaggt agccgatgac cacgtcccgt gctcgggtca ccgtccccgc gtcggcctcc
     2641 tggtcgccgg tgatgatctc gagcagggtc gtcttgccgg cgccattggg gccgacgatc
     2701 gcgaggcggc ggccggcgct gatctgcagg ccgacatcgc tgaacaggtg ctgcgggccg
     2761 tggctcttgg ccacgccggt cagggtcacg aggctcatgg gctgcccaga tggtagccgg
     2821 ggtgtggcgg cgggacgcgg ccggcgtcca gggttctgcc cgtgcgacgg cgattgatgg
     2881 ggttgatcac agcgcaccgg acgggccgtt cccgcgccgg gcggtcggcc ctcgcccggc
     2941 gacgctcagt ccgcgatcag ccgtgctccc gcggtgtcga accgcagccg cgtcgccatc
     3001 cccggcaggc cggccccgtt ccacgccttc tcgacggcgt gcgctccggc gaccgagtcg
     3061 cagatggcca ggacggtcgg cccggccccg gacaggcagg cgccggcggc ccctcgctcg
     3121 tacgccagcc cgatcaggtc acgcgtgccg ggcagcaccg gcagccggta gggctggtgc
     3181 agctcgtccg ccatggccac gcggaggagc ccggcgtccg aggtcatgat cgcggtcgcc
     3241 agcagcgccg tgcggccggc ctggcgcgcg gcggccgcat ggctgaccga tgacggcagg
     3301 gccgctcgca tctcgtgcgt ccggctctcg cgctggggga tgaaggcgac cgggatccac
     3361 gcctccggca cccggaagcg gcgcagggtg atcggctggc cggggtcggc gaccgcgatg
     3421 gtgaagccgc cgtacagggc cgccgcgacg ttgtccgggt ggccctcgag gcgtgaggcc
     3481 agctcgagca gctcgtcggc ctccacctgc cgccgcccca ccgcggcgcc gagcaccacg
     3541 ccggccacga tggcggcggc gctgctgccc agcccacggg ctgccgggat catgctcgag
     3601 acctcgatgc gccacgagcc ggcgcccggg gcggccgaga cgcgcagacc ctcgcggaag
     3661 ccgcggatga gcaggttctc gtcggccggc tcgccgggca gcggtgcgcc ctcgccgcgg
     3721 gcgttgagca ggattccggg ctcgtcgccg tgccacagct cggcgcggag cttgagcgac
     3781 agggcgccgg cgaagcagtc gaagccactt ccgaggttgg cgctcgtggc cgggacgtcg
     3841 accgaggcaa tgggcggcat gcgcggcgag tatatgaggc gacctacgtc atccggccgc
     3901 gcgtcgctca acctacgtcg gagcgcggcg tgcgacaccg caccgtggga tcgaggggcg
     3961 ccgccaccga gactgagcgt catgtcacga accgcccagc ccaccacccg ccgcgccacc
     4021 aggcgtcgcg cggaggcggc accgcaccag cccatgaccg cggaggccgc gctccgcgac
     4081 gacccggtcg tgtggctgag ctcggtccag cccgacggcc ggccgcatgt cgtgccggtc
     4141 tggttccact gggacgggga gcggatcgtg gcgttcagca agccccgcgc gcgcaagatc
     4201 gagaatctgc gcgaacacgc cagcgtgatg ctcgcggtcg gcacgccggg ccccgacttc
     4261 gaggtcgagc tgatcgaggc gaccgctgag ctgcccgacg tcccggccag cgacgtgatt
     4321 cccgacggct tcggtgccaa gtaccgcgag ctgctgcggc gcgccggcct caccgtccag
     4381 aagttcgccg aggtgtactc gcagccgatc gtgctgcggc cgacccgctt cctgggctac
     4441 ggcgggcgcg gctgggatcg cgcgcaggcc gtcgccagct gagccgaccg cctacggcga
     4501 ggtctggact cgctcgatgg cggagatgag gtcgtcgaag ccgaacggct tggccaggta
     4561 gtccgaggcg gcgatctcgg ccgcccaggt ggcggcgtcg cgagccgccg tcatcaccac
     4621 gatcgggatc ctgacgcccc gacggcgcag ctcgtcggcg aagccccagc cgtcgaggac
     4681 gggcatccgc atgtccagca ggatgaggtc gggctcctcg cgctggacga cggccagcgc
     4741 atcggctccg ttggtggcct ccgtcacgcg gtagccctcg atctcgagca tggccgagac
     4801 cgtggcacgg atgtcgggct cgtcgtcgac gacgaggacg gacaggtcag gcgtcggcat
     4861 gggcggaatc ggtactcaat gcgggcgttg ggttccaggc tgcgtccatt ggtggcgcgg
     4921 catcggccgc cggatcgacg gtcatcccat gcgcctcggc gttccggcgc gcgatgggga
     4981 cggagagcgt gaacgtactg ccgccgccgg gcgtcggctc gaaccagatg ccacctccgt
     5041 gcgcctcgac gatgcggcgg cagatgtaca ggcccaggcc gatcccggtg tcggtgatgc
     5101 tgtgcgcgtt cgaggcgcgg aagaatcgct cgaagacgcg ctcgcgctcc ggctccggga
     5161 tgccgatgcc gtggtcgacg accgagacga gggcctgctc gtcggcagcc caggcccgga
     5221 tggagggcgg ggcgtcgcca ccgtacttga gcgcgttctc gaccaggttc tcgacgacct
     5281 gctccatgcg tcccgtatcg gcctcgacgg aaagctccag cgcggcgagg tcggcctcca
     5341 gcgtcgcgcc gtactcgcgg tggcgctcga ccacggtctg gaccacctgg cgcaggtcga
     5401 cgcgctggaa gcgcagcacg gcacgggact cttccaggcg ctgcgcgtcg aggagctcgg
     5461 tgaccaggtc gcgcagtcga tgcgcctcgc gcacgatgcg gccgatgctc gccggatccg
     5521 gcggctcgtt cggccggcgc aggatgcgac gctgcagcag ctcggcctgg ccgagcacaa
     5581 ccgtcagcgg cgtccgcaga tcatgggcgg cggccgagat gaactcctcc ttgagccgtg
     5641 ccgcgtcctc gcgggcacgg aggtcgctgg cctgggcact cagggcgcgg gcctcgagca
     5701 ggacgcccgt gtgcgaggcg agcaggtcca gcagccacag gtcgtcctcg gcgaagatcg
     5761 gcgcgcggct ggcgaagatg gtcagcacgc cgagcggctc gccgtccgtc acgatgggcg
     5821 aggcgatcac ggttcgggcg ccaacccggc ggtacgcctc cacgttctcc gggtcgaagg
     5881 cggcggagtc gagcaccacc acgcgtcgcc gatccgtgaa tgcccgcccg ccgacgtaca
     5941 ggtcgtcggg gtactcctcc cagcccgaac gggccgcgta ccgcagcgtt ccgcgctccg
     6001 ggacggccac accgatgacg gcgccgttgg cgccgaaggc atcggccgcc gcagcctgca
     6061 tctgcgtcag ggccgcgtag tcatccgtga cgccgatgat ctgggtcgag cgctcgagga
     6121 accgccggac gtcggggtcg cgccagacca gccggaccca ccgcggcggg gtgaagccca
     6181 ggaagaaggc gctcaccgcg accagggcca ggagctgcgt caccgggcgg gtggtctgcg
     6241 cgaggtcggg atccagcgca cccacaaagg cgacgagaag cgcagcgatg aacgcgccgg
     6301 ccccgacggt cacggccgtc atgcgtcgcc tggtgatgcc gcgcgtgcgc tggctctgtc
     6361 gcgcgaacgc gacggcggcg tagccgccca cgacgaccag gaaggccatc gtcgcgatca
     6421 ggaccgtctc gttcgtcacc gggaggatga accccaggat gccgatggcc acgaaggcca
     6481 ccagcccggc catctgcacc cagcgcggac tgtcgctgaa gtcgtcgacg agccggagca
     6541 tggccagcgg ggcgacgttg agcaacagga gggtgacgct gccgacccaa ggctgacccg
     6601 agaagccgag ccagtccgcc agccgcccga gaatgacgac cgaggcggcc gacccgaaga
     6661 gcagcagcgc atccagccgc gtccgcgtcg gctgccgcac ggcagcgacc agcacggcca
     6721 cgaagaggcc gatgaagagg acctggttca ccaggccgat caactcgagg gcagtcatct
     6781 gggtccgcgt acgcgatcgg caggcaacgg ctgcacgtcg catgccacga cgggaagcgc
     6841 ttgctcggcg gtggtgcgtg ctggcgccat cacgacttcg ccgcgaggcc gtgccggaag
     6901 cacgtgacca ggtgatcgtc gacaaggccg gccgactgca tgaacgcgta gcagatggtc
     6961 ggcccgacga accggaagcc gcggtcgcgc agcagccggg agagggcgtc ggaggcgccc
     7021 gtctgtgccg ggatctgctc catgagcgtc caccggttga ccacggtccg gccgtccacg
     7081 acggaccaca cgagatcgac gaagtcggga atggccagga acgcccgcgc attgccgatg
     7141 aacgcgtcga tcttcgcccg gttgcgcacg atgcgcgcat cggtcatgag gcgctctcga
     7201 tccgcgtcgt cgtatccggc gatgcgatgg gggtcgaacc cgtcgaaggc ggcacggtag
     7261 ccatcacgct tgcggaggat ggtgatccac gccagcccgg cctgggcgcc ctcgaggcag
     7321 agcagctcga acagccgatg cggatcgcgc agcggcacgc cccactcggc atcgtggtag
     7381 tcggtcatga gcgggtcgcc ggtcgcccag tcgcagcgag ccgtggtctc atcgagcgtc
     7441 aagggcgccg aggatagcgg cagcggaacg cgcgggtcga gcgacccggg cgtcatcggt
     7501 gctggtgctg gtgctggtgc tggcggcgcg ttgccggtgg gcccaacatc ggcgcggttc
     7561 aggcgcgtga tgcggagtcg gccaggcgag ggcgcgcgct gcggatccgg tcgagccacg
     7621 aacgcgactc ggtgcggtcc tcgcggcggg ccgccttcgc cagctgtcgc tggcgaagct
     7681 gacggcggta gtcgtcctcg gtagcccgcg ccagcaggag cgcggcggtg gggttcatcg
     7741 gtcggcctcc tcggaaggcg tacggtggtg gaacggtcag cggagaaacg cccggagcac
     7801 gctccgcgcg atctcggcgg ttcgccggct cctccgttcc ggccggtgcg gccgatgcgg
     7861 aggcggcgag aagaacgcga cgtccggcgc cgtggtggcc cacgtgccga gtgccatcgt
     7921 catggctgcc tcctttcctg gtgtggtctc ggtatcggat ggatgcgact catgctggtc
     7981 ggacgagcag gtgcttccaa cggtcggcgg tcgcggtcgg gcgcggatca tcggccgagc
     8041 gatccgtacg tcattccgcc gccgtcactc accggttcgc gggcctatcc tgtgaccatg
     8101 gcgcccgagc tcgctccgca gcacctgagc ttcctgttcg ccgacgtgga ggggagcacg
     8161 cggatgacgg agcagtacgg tgtcgtcgcg ggcgccgccc tgacgcgcta ccacgagatc
     8221 gtggccgagc gggcggccgc gcacggcgga cgcatcttcg agcgcatcgg tgacggcgcc
     8281 tacgcctgct tcgacgcggc tcgcgaggcg gtgcaggccg cggacgacat ccagggcgtc
     8341 atgacggaca ccgactgggg cgagatcggg cgggtccgcg ttcggctggt ggtggtcagc
     8401 ggcgaggtcg aagtgcgcgg cgaccggttc ttcgggctgc cgctgtaccg cgccgcgcgc
     8461 ctgcaggccc tcgccgacgg gggagagacg ctccttgccg gcaccacggt ggacgagctc
     8521 ggcgggtggc tgccggacgg cacgatgctc cgcgacctcg gcgcgcatcg gctgcgcgac
     8581 atggtggagc ccgagcgcat cttcgcgctg gtgcatactt cccgcgcgcg tctcggcgac
     8641 gagccgccgc ccgagccatc ccggattcag gggaccgaga ctgtgactga cgaagaaggg
     8701 ccgattcgcg tcctgctggt ggacgatcac gccgtcgtcc ggcgcggact gcggggcttc
     8761 ctggagctgt tgaaggactt cgacgtcgtc ggcgaggcgg agaacggccg cgaaggcgtc
     8821 gccgccgccg accgcctggt gcccgacgtc atcctcatgg acctgctcat gccggagatg
     8881 ggtggcctgg aggcgatcgc cgccatcaag caggcgcacc cggagatcga gatcgtggcc
     8941 gtcacctcct tcatcgagga ggagaaggtc acctcggcgc tcgaggcggg cgcgtcgggc
     9001 tacctgctca aggacgc
//
LOCUS       DASTYP010000102         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1136420, whole genome shotgun sequence.
ACCESSION   DASTYP010000102 DASTYP010000000
VERSION     DASTYP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136420"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            621..872
                     /locus_tag="VFV59_04975"
                     /pseudo
     CDS             621..872
                     /locus_tag="VFV59_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683305.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
     gene            1195..1638
                     /locus_tag="VFV59_04980"
     CDS             1195..1638
                     /locus_tag="VFV59_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5325217.1"
                     /translation="MLEPVRIGHVVLKVRDLDRALAFYRDLLGFRVASTMSNVMIFLT
                     ATGESHHDLALLRVGDSAPSPLPSAVGLYHVAVQLRDWEAVKRAHALLSERGLLKGTA
                     DHGVTKSLYTVDPDGNEIELFCDAPREEWEGRVAEAMTVRPLDIG"
     gene            1676..2698
                     /locus_tag="VFV59_04985"
     CDS             1676..2698
                     /locus_tag="VFV59_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325218.1"
                     /translation="MPFLPVVLLLAWQAISKSASFALGWATALYFGQVPGRQGRILSV
                     ISLLAAGWVIVVVGFAVPIFTGAILEATGVIEENFDVRWIHYLGLVAAIALTPPIVAG
                     TAVWGEFHEDRSWRRWLRMIPMSYPATAMLGLSVLQMVVITPFLLVMRWRQKRKLVQV
                     PLIMHSGCDDDDMVELVRRALATIDIDDVSAAEAEGPRSWPLRTIGFAAEHLLGAVVR
                     GEPMRMRADGLEILAYATNIAVLGPKEDAYRARAAILRTLTFADAYLTWSDDARDFED
                     ALRRAHDEADGDLDELRDRFDEVQAKMDVASLNSEEWNVLYRIRLELEADAARRDDGA
                     SRRMTA"
     gene            complement(2695..2949)
                     /locus_tag="VFV59_04990"
     CDS             complement(2695..2949)
                     /locus_tag="VFV59_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325219.1"
                     /translation="MAALAPAFALGALDPDEHGFLKEHRATCGLAHPELREGLVLASI
                     FGAAEAVRTRPRPCLRRQVLDAIHAEALAAPRHREASDRR"
     gene            complement(3030..3182)
                     /locus_tag="VFV59_04995"
     CDS             complement(3030..3182)
                     /locus_tag="VFV59_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325220.1"
                     /translation="MSRSPRRRKGRPYQPLPQRPGGAAWGARLLVLAVGILLILGSLA
                     LFGGLR"
     gene            complement(3179..>3508)
                     /locus_tag="VFV59_05000"
     CDS             complement(3179..>3508)
                     /locus_tag="VFV59_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325221.1"
                     /translation="ERIPGTMERLSTLVEDVDASFDNAMITLDNERRSRRFVAIDEAK
                     AILDRWLPPSVIADVREWDVARLHEPYGDGTYHGKLLKRTRKQLRSVSSRDALTVPFL
                     FRRSWSS"
BASE COUNT          485 a         1259 c         1236 g          528 t
ORIGIN      
        1 ccggtgaccg cctcgtgcga acggaggagc ttctggtcct cggtccggcg gtctccgggc
       61 gacgtgccac gcacgacaac ggcgcctcga cggcgctcgg gctgcttctt ccgacgtggc
      121 atcgacaact ccgcaactcg acaggcccgg aaccgggtgc tagggtggcc cgcgccggca
      181 cggaatccgc agggatcgtg ccggctggac cacgtactcg ggaagattct attgccggac
      241 catccgcggc acctcgccgc cgtcaccccg cgggggcgct gaccatgtcg attcttcgac
      301 ggatcgcggc gctcgccgtc atcagcctca ccggtgtggc cctgctggcg cccctggccg
      361 ctcgctccgc cgccgcacct gtcccggtgg acctgccgga gcagctgctc ccagcctcgt
      421 ccgccgctcc cctcctcgcc gatcgcagcc ttctctccgc gcgatccgcg agcctgacca
      481 ccaccgccgc gacctctgag gagatcgcga ccccggcggt cccggccccg gcatcggtct
      541 cgatgcaggc gaccctcgtc accatcccga ccccgaagcc ggcacccgtg gccgccgcac
      601 cccagcgcgt cgtcacctac gacggcaaca ccgtctggga ccagctggcg cagtgcgagt
      661 cgggcggcaa ctgggccatc aacacgggca acggttacta cggtggcctg cagttcaacc
      721 tcgggacgtg ggaggcgtac ggcgggctcg agttcgccga gcgcccggac ctcgcgaccc
      781 gggagcagca gatcatcgtc gccgagcggc tccacgcggc gcgcggcttc cagccgtggc
      841 cggcgtgccg catcaagctg ggtctgccgt aaccgctcgt ctccgtctgg tggcggccgc
      901 cccgccgctg gccgcggctc cgactccgcc atccacgccg tgagggcgtg actggcgctg
      961 gccgcggttc cggctccgcc atccacgccg tgagggcgtg agcgacgtca ttcgggcgcc
     1021 acgagtgcgc caaatccgcc ggattcacgc gtgggaggcg tgaggtggcg gcgtcgcggc
     1081 ggtgatcgcc gaggttcacc acccccgggc gtgaatggcg agatccggat gcagccgacg
     1141 ggtacggacg ggaatggcgc gaagggggtg gcgtgggccg gtagcatcgg cggcatgctc
     1201 gaacccgtcc gcatcggtca tgtcgtcctc aaggttcgcg atctcgatcg cgcgctggcc
     1261 ttctaccgcg atctgctcgg gttccgcgtg gcgagcacga tgagcaacgt gatgatcttc
     1321 ctgaccgcga ccggggagag ccaccacgac ctcgcgctgc tgcgggtcgg cgactcggcg
     1381 ccgtcacccc tccccagcgc ggtcgggctg taccacgtcg ccgtgcagct gcgcgactgg
     1441 gaggcggtga agcgcgcgca cgccctcctg agcgagcgag ggctgctgaa gggcacggcc
     1501 gaccacggag tgacgaagag cctctacacc gtcgacccgg atggcaacga gatcgagctg
     1561 ttctgcgacg cgccccgcga ggagtgggag ggtcgtgtgg cggaggccat gacggtgcgg
     1621 ccgctcgaca tcggctagcc gcggcaccgg cacgtcgctt gcttggtcac cgttcgtgcc
     1681 gttcctcccc gtcgtcctgc tgctggcgtg gcaggcgatc agcaagtccg catccttcgc
     1741 gctcggttgg gcgaccgccc tgtacttcgg acaggtgccc ggccgccagg gccggatcct
     1801 gagcgtcatc agcctgctcg ccgcgggctg ggtgatcgtg gtggtgggct tcgcggtccc
     1861 gatcttcacc ggcgccatcc tcgaggcgac cggcgtgatc gaggagaact tcgacgtccg
     1921 ctggatccac tacctcggtc tcgtggcggc catcgcgctg acgccgccca tcgtcgccgg
     1981 caccgcggtc tggggcgagt tccacgagga ccggagctgg cggcgctggc ttcggatgat
     2041 tcccatgagc taccccgcca cggcgatgct ggggctatcc gtgctccaga tggtcgtgat
     2101 cacgcccttc ctgctggtca tgcgctggcg gcagaagcgg aagctggtcc aggtgccgct
     2161 gatcatgcat tccggctgcg acgacgatga catggtcgag ctggtgcgcc gggcgctggc
     2221 gaccatcgac atcgacgacg tctcggcggc cgaggccgaa ggcccgcgct cctggcccct
     2281 ccgcaccatc ggcttcgccg ccgagcacct gctgggtgcc gtcgtccggg gtgagccgat
     2341 gcggatgcgc gccgacggcc tcgagatcct ggcctacgcc acgaatatcg ccgtgctggg
     2401 tccgaaggag gacgcctacc gcgcccgggc ggcgatcctg cgcacgctca ccttcgcgga
     2461 cgcgtacctg acctggagcg acgacgcgcg agacttcgag gacgcgctgc ggcgggcgca
     2521 cgacgaggcc gatggcgacc tcgacgagtt gcgcgaccgg ttcgacgagg tgcaggccaa
     2581 gatggacgtc gccagcctca actccgaaga gtggaacgtc ctctaccgca tccgcctgga
     2641 gctggaggcc gatgcggccc gccgcgacga cggcgcatca cgccgcatga cggcctagcg
     2701 ccggtccgac gcctcgcggt ggcggggagc ggcgagtgcc tcggcgtgga tcgcgtcgag
     2761 cacctggcgt cgcaggcatg ggcgcggtcg cgttcgcacg gcctccgcgg cgccgaagat
     2821 cgaggcgagg accagtccct cgcggagctc ggggtgcgcg aggccgcagg tcgctcggtg
     2881 ctccttcagg aagccgtgct cgtcgggatc gagggctccg agcgcgaacg ccggcgcgag
     2941 ggccgccact cgggcgcaat cgtgaccgtt cacgctctcc ctacggacgc gcgtcctcgc
     3001 cggatcatcc gggggaggac cgccccgcgc tacctcaggc cgccgaagag cgccaggctg
     3061 ccgaggatca gcaggatgcc cacggccagc accagcagcc gggcgcccca cgccgcaccc
     3121 ccaggccgct ggggcagcgg ctggtacggc cggcccttgc gtcgtcgcgg cgagcggctc
     3181 acgaggacca gctccgtcgg aagaggaagg gcacggtcag cgcgtctcga ctcgaaacgg
     3241 atcgcagctg ctttcgcgtt cgcttgagca gcttgccgtg gtacgtgccg tcaccgtagg
     3301 gctcgtggag tcgggcgacg tcccactccc gcacgtcggc gatcacggac ggcggcagcc
     3361 atcggtccag gatggccttg gcctcgtcga tcgccacgaa ccgccgtgag cgccgctcgt
     3421 tgtcgagggt gatcatcgcg ttgtcgaagc tggcgtcgac gtcctcgacc agggtgctca
     3481 gccgctccat ggtgccgggg atgcgctc
//
LOCUS       DASTYP010000103         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1099895, whole genome shotgun sequence.
ACCESSION   DASTYP010000103 DASTYP010000000
VERSION     DASTYP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099895"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="VFV59_05005"
     CDS             complement(<1..2033)
                     /locus_tag="VFV59_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5325222.1"
                     /translation="MDRFDKFTDRARKVLTLAQDEAQRFNHNYIGTEHLLLGLVREGE
                     GVAARVLENMNVELSKVRTAVEFIIGRGDRPVVGEVGLTPRAKRVIELAIDEARRLGH
                     NYIGTEHLLLGLVREGEGIAAGVLESLGVNLDKVRHQVIHVLSQSSTATPAQETKRPS
                     KTPTVDQLGINLTDAARAGTLDPVIGREKEIERVIQILGRKTKNNPALIGEPGVGKTA
                     IAEALAQRIVSGDVPETLLDKRVLTLDIGSLVAGTKYRGEFEERLKKIIEELRSSSDS
                     VLFIDELHTLVGAGAAEGAIDAANILKPPLSRGELQCIGATTLDEYRKYIEKDAALER
                     RFQPVMVEEPTVDEAIDILFGIRERYEQHHRVKISDEAIHAAVDLSVRYVADRALPDK
                     AIDLVDEAGSRVRLRSASAPAEIKAATQALEEITRQKDEAIAGQDYEAAARLRDEEAQ
                     GKERIEEMRKAWHEEQAKETPVVTDEDIAQVVSMWTGIPVIRISAEESERLLKMEEAL
                     HGRVIGQQEAIETISKAVRRARAGLKDPKRPIGSFIFMGPTGVGKTELAKALAEFMFG
                     SEDALVKIDMSEFMERHNVARLVGAPPGYVGYDEGGQLTEAVRRKSYSVVLLDEIEKA
                     HPEVFNILLQILEDGHLTDAKGRRVDFRNTVIIMTSNVGAQQLQRDTSIGFRAG"
     gene            complement(2275..>3384)
                     /locus_tag="VFV59_05010"
     CDS             complement(2275..>3384)
                     /locus_tag="VFV59_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU5325223.1"
                     /translation="WRAMQERFEAGEIVEARVIDHNKGGLIVDLGVRGFVPISQIVDF
                     PRRPRDEQPRDAAQEIAEKLQPFVGRTLRLKILEVNRKANRLILSEKVALYEERREKR
                     DELFSSLQVGQRVMGIVRSIAPFGVFVDLGGIDGLVHKSELSWNKVNNPETAYQIGDE
                     VEAEVIDINHERGRISLSIRRLQPDPWQESVAKYKIGDVIEGTVTKLVNFGAFVRVEE
                     GLEGLIHISELSHQRVAHPGDVVQEGQQVKLRIISLDSERHRLGLSLKQAEDRPAPAP
                     REDRPRRDRGGRRDRYDYRPEDATAEPEGGIDNTMAAAFAESGLLEQFRQSQGSASVE
                     TEVEAEDQTEVVDQTTAEDQTAAEDQTAAEGDEQA"
BASE COUNT          431 a         1185 c         1138 g          630 t
ORIGIN      
        1 ccggcccgga agccgatgct cgtgtcgcgc tgcagctgct gcgcgccgac gttgctggtc
       61 atgatgatga ccgtgttgcg gaagtcgacg cggcggccct tcgcatcggt caggtggccg
      121 tcctcgagga tctgcagcag gatgttgaag acctcggggt gcgccttctc gatctcgtcc
      181 agcaggacca cgctgtagct cttgcgccgc accgcctcgg tcagctggcc accctcgtcg
      241 tagccgacgt agccgggcgg agcgccgacc aggcgcgcca cgttgtgccg ctccatgaac
      301 tcgctcatgt cgatcttcac gagcgcgtcc tcggagccga acatgaactc ggcgagcgcc
      361 ttggccagct cggtcttccc gacgccggtg gggcccatga agatgaacga gccgatcggc
      421 cgctttgggt ccttcaggcc ggcgcgcgca cggcgcaccg ccttgctgat ggtttcgatg
      481 gcctcctgct gcccgatgac gcggccgtgc agcgcctcct ccatcttgag cagccgctcg
      541 gactcctcgg cgctgatgcg gatgaccggg atgccggtcc acatgctcac gacctgggcg
      601 atgtcctcgt cggtgacgac cggcgtctcc ttcgcctgct cctcgtgcca cgccttgcgc
      661 atctcctcga tgcgctcctt gccctgtgcc tcctcgtccc gcaaccgcgc cgcggcctcg
      721 tagtcctggc cggcgatggc ctcgtccttc tggcgcgtga tctcctccag cgcctgcgtc
      781 gcggccttga tctccgccgg agccgatgcg gaccgcagcc gcacccggga cccggcctcg
      841 tcgaccaggt cgatcgcctt gtccggcagg gcgcggtcgg ccacgtagcg gaccgacagg
      901 tcgacggcgg cgtggatcgc ctcgtcgctg atcttcacgc gatggtgctg ttcgtagcgc
      961 tcccggatgc cgaagaggat gtcgatcgcc tcgtcgacgg tcggctcctc caccatgacc
     1021 ggctggaatc gccgctcgag cgccgcgtcc ttctcgatgt acttgcggta ctcgtccagc
     1081 gtcgtggcgc cgatgcactg cagctcaccg cgggatagcg gcggcttcag gatgttggcc
     1141 gcgtcgatgg cgccctcggc cgctccggca cccaccaggg tgtggagctc gtcgatgaac
     1201 agcacgctgt ccgagctgga gcgcagctcc tcgatgatct tcttgaggcg ctcctcgaac
     1261 tcgccgcggt acttggttcc cgccacgagc gagccgatgt ccagcgtcag cacgcgcttg
     1321 tcgagcagcg tctccggcac gtcgccggac acgatgcgct gcgccagcgc ctccgcgatg
     1381 gccgtcttgc ccacaccagg ctcgccgatg agcgcggggt tgttcttcgt cttgcgcccc
     1441 aggatctgga tgacgcgctc gatctccttc tcgcgcccga tgaccgggtc gagggtgccg
     1501 gcgcgtgccg catcggtcag gttgatgccc agctggtcga cggtcggcgt cttcgacggc
     1561 cgcttcgtct cctgcgccgg cgtggcggtg ctcgactggc tcaggacgtg gatgacctgg
     1621 tggcgcacct tgtcgaggtt cacgcccagg ctctcgagca cgccggcggc gatgccctcg
     1681 ccctcgcgca ccagtccgag cagcaggtgc tcggtgccga tgtagttgtg cccgaggcgg
     1741 cgcgcctcgt cgatggccag ctcgatgacg cgcttggcgc ggggcgtcag gccgacctcg
     1801 ccgaccaccg gccggtcgcc gcgtccgatg atgaactcga ccgcggtgcg gaccttcgac
     1861 agctcgacgt tcatgttctc gagcacgcgc gcggcgacac cctcgccctc acgcacgagg
     1921 ccgaggagga gatgctcggt gccgatgtag ttgtggttga accgctgggc ctcgtcctgc
     1981 gcgagggtga ggaccttgcg ggctcgatcg gtgaacttgt cgaatcggtc cattggggcg
     2041 ctttccagat gcgtctgagt cgagtgtacg gcacgcggtg cgcgagttgc tgccgcgcat
     2101 cgctgccgat ggccgtgtga agagctatac ggacgacgac acgtccgggc tcatttgttc
     2161 cgcgtctcga cgcggattcg tgccccctgt ggctggccgg aatgcaggaa gcggacccgc
     2221 acgggcccgc ttccgcggtc gcctggtgtg cggccgtggc ggccgcgccg ggtatcaggc
     2281 ctgctcgtcg ccctcggccg cggtctggtc ctcggccgcc gtctgatcct cggccgtggt
     2341 ctggtccaca acctcggtct gatcctcggc ctcgacctcg gtctcgacgg atgccgagcc
     2401 ctgcgactgg cggaactgct cgaggaggcc cgactccgcg aaggctgcgg ccatcgtgtt
     2461 gtcgatcccg ccctcgggct ccgcggtggc gtcctcggga cggtagtcgt accggtctcg
     2521 acggccgccg cgatcgcgcc gcggtcgatc ctcgcgcgga gccggcgccg gacgatcctc
     2581 ggcctgcttc aggctgaggc ccaggcgatg ccgctccgag tccaggctga tgatccgcag
     2641 cttgacctgc tggccctcct ggacgacgtc gccgggatgg gcgacgcgct ggtgggacag
     2701 ctcgctgatg tggatcaggc cctcgagccc ctcctcgacg cggacgaagg caccgaagtt
     2761 gacgagcttg gtgacggtcc cctcgatgac gtcgccaatc ttgtacttcg ccaccgactc
     2821 ctgccacgga tcgggctgga ggcgccggat cgagaggctg atacggccac gctcgtggtt
     2881 gatgtcaatg acctcggcct cgacctcgtc gccgatctgg tacgcggtct ccgggttgtt
     2941 gactttgttc cacgagagct cgctcttgtg gaccaggcca tcgatcccgc ccaggtcgac
     3001 gaagacgccg aacggcgcga tgctgcggac gatgcccatg acccgctgcc cgacctgcag
     3061 gctgctgaac agctcgtcgc gcttctcgcg gcgctcctcg tagagggcca ccttctccga
     3121 gaggatcagg cggttggcct tgcggttcac ctcgaggatc ttcaggcgca gcgtgcggcc
     3181 gacgaacggc tgcagcttct ccgcgatctc ctgggcggcg tcgcgtggct gctcgtcgcg
     3241 cggacggcgg gggaagtcga cgatctggct gatcggcacg aagccgcgca cgccgaggtc
     3301 gacgatgagg ccgcccttgt tgtggtcgat gacccgcgcc tcgacgatct cgcccgcctc
     3361 gaagcgctcc tgcatcgcgc gcca
//
LOCUS       DASTYP010000104         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1317796, whole genome shotgun sequence.
ACCESSION   DASTYP010000104 DASTYP010000000
VERSION     DASTYP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317796"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /gene="topA"
                     /locus_tag="VFV59_05015"
     CDS             complement(<1..1940)
                     /gene="topA"
                     /locus_tag="VFV59_05015"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU5325224.1"
                     /translation="MARPLVIVESPAKARTLARLLGDDYRVEASVGHVRDLPQNASEI
                     PAEIRDKPWASIGVDVEHGFRPYWVVSRDKTGRIRELRAAMKGVEEVLLATDPDREGE
                     SISWHLREVLAPKVPVRRIEFHEITEEAVRRAIANAREIDLRLVDAQESRRIVDRLYG
                     YMVSPVLWKKVQTGLSAGRVQSVAVRLIVEREEARRAFRSSAYWGLEAKIGAETGGFT
                     ATLNRVGDRRVATGRDFDTTTGELRERSVLRLDEATAGALRDDLADRLPWTVTAVEER
                     PVTQSPAPPFTTSTLQQEANRKLGFSADRTMNAAQRLFQEGIISYHRTDSVSLSERAL
                     GEAASAIRSLYGDEVYRGARQYRTRVKNAQEAHEAIRPTDFGRTPQRFGQRLGSDEAR
                     LYELIWKRAVASQMADARLLRTNVEITASGTDGPAVFGASGKTVQFPGFLRAYVEGSD
                     DPAAELSDQETVLPRLAVGDTIHAEGPLTLEGLDARSHETTPPARYTDASLVKRLEED
                     GIGRPSTYASIISTIERRGYVFRQGKALVPTFTAFAVVHLLKEHFAELIELGFTGQIE
                     ENLDEISKGELARDEFLAEFYEGDPEHDWPGLRPLVENETQIEYPVVQLGEHPQTGAP
                     VVVRIGRFGPYVQVGEGDDAINA"
     gene            complement(2149..>3213)
                     /locus_tag="VFV59_05020"
     CDS             complement(2149..>3213)
                     /locus_tag="VFV59_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5325225.1"
                     /translation="ATDVSATTSITIGFSAEMDEADVESRFAIAPDVAGDLTWDAGQL
                     VFTPTERLVPGARYTISVNGAHDRRGNALGGELNFSFIVQAGAQLITTDPKLGASDVE
                     PATVEMWFSQPMDVDATNAAFALVDTTTGAPVGGHLVWNEAGTQVIYTPDQPLAGGRT
                     FDVTIGDGGLDAAGNPVTASWSFTTKAAPVATTERSSTAASTRSATAAPAPVIPPPAP
                     ASDAAGYAVNQINAARAAYGFGPVVLDPTISAVAYGHAYDQAINNYFSHTGLDGSTRD
                     SRLRAGGVSFGWSGENQCYLIGRSVTATLDWCHSAFMSEPYPGYWNHIANVLNPNAHR
                     VGVGIAQVGGKIVIVWDFTD"
BASE COUNT          430 a         1140 c         1138 g          505 t
ORIGIN      
        1 gcgttgatgg cgtcgtcgcc ctcgccgacc tgcacgtacg gtccgaatcg accgatgcgc
       61 acgaccaccg gcgcgcccgt ctggggatgc tccccgagct gcacgaccgg gtactcgatc
      121 tgggtctcgt tctcgaccag cggtcgcagc cccggccagt cgtgctccgg gtcgccttcg
      181 tagaactcag cgaggaactc gtcgcgcgcc agctcgccct tgctgatctc gtcgaggttc
      241 tcctcgatct ggccggtgaa ccccagctcg atgagctcgg cgaagtgctc cttcagcagg
      301 tggaccacgg cgaacgccgt gaaggtcggc accagggcct tcccctgccg gaagacgtag
      361 ccgcgccgct cgatggtcga gatgatcgag gcgtacgtcg acgggcggcc gatgccgtcc
      421 tcctcgaggc gcttgaccag cgacgcatcg gtgtagcgag cggggggtgt cgtctcgtgc
      481 gaccgcgcgt ccagcccctc cagcgtcagc ggtccctcag cgtggatggt gtcgcccacc
      541 gcgaggcgtg gcaggacggt ctcctggtca ctcagctccg cggccgggtc gtcggatccc
      601 tccacgtacg cgcgcaggaa gcccgggaac tgcaccgtct tgccggatgc accgaacacc
      661 gccggcccat cggtgcccga cgcggtgatc tcgacgttgg tgcggagcag gcgagcgtcc
      721 gccatctgcg aggccaccgc gcgcttccag atcagctcgt agagccgggc ctcgtcgctg
      781 cccagccgct gaccgaagcg ctgcggcgtt cggccgaagt cggtgggccg gatggcctcg
      841 tgcgcctcct gggcgttctt gacccgggtg cggtactggc gcgctccccg gtatacctcg
      901 tcgccgtaca gggagcggat ggcagatgcc gcctcgccca gcgcccgctc gctcagcgac
      961 accgagtcgg tgcggtggta cgagatgatc ccctcctgga agaggcgttg cgccgcgttc
     1021 atggtgcggt ccgcgctgaa gccgagcttg cggttcgcct cctgctgcag cgttgaggtg
     1081 gtgaacggcg gcgccggcga ctgcgtgacc ggccgctcct cgacggccgt caccgtccac
     1141 ggcagccgat ccgccaggtc gtcgcgcaac gcgccggccg tcgcctcgtc gagccgcagg
     1201 accgatcgct cgcggagctc gcccgtggtg gtatcgaagt cccggccggt ggcgacccgc
     1261 cggtcgccga cgcggttcag cgtggcggtg aagccgcccg tctccgcgcc gatcttcgcc
     1321 tcgaggcccc agtacgcgct cgagcggaag gcgcgacgtg cctcctcgcg ctcgacgatc
     1381 aggcgcaccg cgacgctctg cacgcgaccg gcactgaggc cggtctgcac cttcttccac
     1441 agcaccggcg agaccatgta gccgtacagc cggtccacga tgcgccggct ctcctgcgcg
     1501 tcgaccagcc gcaggtcgat ctcccgcgcg ttcgcgatgg cgcgccgcac cgcctcctcg
     1561 gtgatctcgt ggaactcgat gcgccggacg ggcaccttcg gggcgaggac ctcgcgcagg
     1621 tgccagctga tcgactcgcc ctcgcggtcg gggtcggtcg ccaggagaac ctcctcgacg
     1681 cccttcatgg ccgcccgcag ctcgcggatg cggccggtct tgtcgcgcga gacgacccag
     1741 tacggccgga agccgtgctc gacgtcgacg ccgatgctgg cccacggctt gtcgcggatc
     1801 tcggcgggga tctcgctggc gttctgcggc aggtcccgca cgtgcccgac gctggcctcg
     1861 acgcggtagt cgtcgccgag gagccgcgcc agcgtcctcg ccttggccgg tgactcgacg
     1921 atgaccagcg gcctggccat cagcgaccag cccggaacgg tgtcgtcgtg cccacggcga
     1981 gcgcctcgac gagcgcgagg tcgccggcgg cgttcatcgt gcgcggcatc ggtcggagag
     2041 tgtagcaccg gcccaggccc accccgccca tggcggcgag gggcccggga gccggtcgat
     2101 accgggtggg agcggcacgg gcggccgagc gccgcccgtg tccgtcggtc agtcggtgaa
     2161 gtcccagacg atgacgatct tgccgccgac ctgtgcgatg ccgaccccga cccgatgtgc
     2221 attcggattg aggacgttcg cgatgtggtt ccagtagccg ggatacggct cggacatgaa
     2281 ggcgctgtgg caccagtcga gcgtggcggt cacgctgcgc ccgatcaagt agcactggtt
     2341 ctcgcccgac cacccaaagc tgaccccgcc ggcgcgcagc cgcgagtcgc gggtgctgcc
     2401 gtcgagcccg gtgtggctga agtagttgtt gatggcctga tcgtaggcat gcccgtaggc
     2461 caccgccgag atcgtcgggt cgagaacgac cgggccgaaa ccataggccg cgcgcgccgc
     2521 gttgatctgg ttgacggcgt atccagccgc gtccgacgcg ggggccggag gcgggatgac
     2581 cggcgcgggc gccgccgtcg ccgaacgcgt cgaggccgcc gtcgaagagc gctcggtggt
     2641 cgccaccggg gcggccttcg tggtgaacga ccacgacgcc gtcacggggt tgccggccgc
     2701 gtcgagaccg ccgtccccga tcgtcacgtc gaaggttcgc ccgcccgcga gcggctggtc
     2761 cggcgtgtag atcacctgcg tgccggcctc gttccagacg aggtgcccgc cgaccggcgc
     2821 gccggtggtc gtgtcgacca gggcgaacgc ggcgttcgtg gcgtcgacat ccatcggctg
     2881 gctgaaccac atctccaccg tcgccggctc gacgtcgctg gcgccgagct tcgggtcggt
     2941 ggtgatgagc tgcgcaccgg cctgcacgat gaagctgaag ttcagctcgc ctccgagcgc
     3001 gttgccgcgg cggtcgtgcg cgccgttgac gctgatggtg tagcgagcgc cgggaacgag
     3061 gcgctcggtc ggcgtaaaga ccagttggcc ggcgtcccac gtcaggtcgc cggcgacgtc
     3121 cggcgcgatc gcgaaacgtg actcgacgtc tgcctcgtcc atctcggcgc tgaagccgat
     3181 ggtgatcgac gtggtggcgc tcacatccgt cgc
//
LOCUS       DASTYP010000105         5723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1027412, whole genome shotgun sequence.
ACCESSION   DASTYP010000105 DASTYP010000000
VERSION     DASTYP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027412"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VFV59_05025"
     CDS             complement(<1..1496)
                     /locus_tag="VFV59_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5325226.1"
                     /translation="MNDEEGLTVARRETTDDLDALMEALPPEICERLRALPDRADLLE
                     VVMDLGRRPEARTTTGEVDLLDREITEADIAHVIEHIGVFGDDNRAGIERTLHRISAI
                     RNRAGKVVGLTCRIGRAVFGTIDIIRDIVEGGQSVLLLGRPGVGKTTMLREVARVLAD
                     DLGKRVVVVDTSNEIAGDGDIPHPGIGDARRMQVRTPTDQHSVMIEAVENHMPEVIVI
                     DEIGTELEAAAARTIAERGVQLVGTAHGNTLDNLMLNPTLSDLVGGIQPVTLGDEEAR
                     RRGTQKTVLERKAPPTFDVLVEIVERDSVIVHRNVAETVDAILRGHMVPPEARYRDDG
                     GELKAQTKYDYRISETAAGNPAFAPIEPTGGFGAFGRGGGRPGSPGGRYERGGLRPLP
                     GGGDVVAGGGRARTLPGERLTGGTSGTTGPGTGGGTAGADPRQELARAISEMQEPGVD
                     RPIGAQRPATPGDRARNQRAMHVFPFGVSRKRLEQAVRELGVPVSVAR"
     gene            complement(1500..2552)
                     /locus_tag="VFV59_05030"
     CDS             complement(1500..2552)
                     /locus_tag="VFV59_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325227.1"
                     /translation="MIEVERTIRAARLTDRQALADLSRRVHTSPEAHRRSLGVPAPTT
                     DSPRISLSSLMPSWLPLRAPSVHLVAEVDGELAGSCRAIEEPHRDDWVITELDAAEGP
                     MAAEVRFHLLNALLEEGVRHDVGRFHAACADVRDNIELFGQAGFMAYAQEEIWWRPPE
                     VVRGPRSWLRRTVSRDGRRPRRDPDGLALQPAGAPDAWHLFDLWTHATPPAIARIEGY
                     GASDWESAGNEAIVPRSSLNPILHFGEVDAWLLPHEQRVAGFAQHGSCRSGPHYLRFL
                     VRDGMDGAAFVGAVIDRLSTDALAAGILAPVRTYESAGTRAAEATGFEPIGRVTLLVR
                     EVRAQVRQPAMVAVTQ"
     gene            complement(2549..3160)
                     /gene="pdxT"
                     /locus_tag="VFV59_05035"
     CDS             complement(2549..3160)
                     /gene="pdxT"
                     /locus_tag="VFV59_05035"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU5325228.1"
                     /translation="MTSAGRTPRIGVLALQGAVREHVDAIRDVGAEPVEVRLPRDLVD
                     LDALILPGGESTTMRRLIDEYGLREPIAALARGGAPMLGTCAGMILLADRIADGDEPM
                     FRALDLEVRRNGYGRQLDSFEADLDAPALGADPLHAVFIRAPQVVDVGPAAEVLARDP
                     DGRPVAVRQGRVIATAFHPELTGDRRLHRLLVDLIGSEAGRTA"
     gene            complement(3174..4055)
                     /gene="pdxS"
                     /locus_tag="VFV59_05040"
     CDS             complement(3174..4055)
                     /gene="pdxS"
                     /locus_tag="VFV59_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU5325229.1"
                     /translation="MERSTWATKVGLARMLSGGVIMDVVTADQARIAEEAGAVAVMAL
                     ERVPSDIRKFGGVARMSDPKLIEEIKAAVTIPVMAKCRIGHFAEAQILQALEVDFIDE
                     SEVLTPADEEHHIDKHRFDIPFVCGCRDLGEALRRIGEGAAMIRTKGEAGTGDIVEAV
                     RHMRAVQKGIRQLQSMREDELFAAAKELRAPLDVVKQTAQLGSLPVPNFSAGGVATPA
                     DASLMRQLGAEAVFVGSGIFKSENPERYARAIVRAATHYDNPDILAEVSAGLGAPMLG
                     ISSGEIPPEERLAVRGW"
     gene            4224..4523
                     /locus_tag="VFV59_05045"
     CDS             4224..4523
                     /locus_tag="VFV59_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU5325230.1"
                     /translation="MPDVTLPVRNPSGLHARPAALFVKTAAGFAADVRVTNLTRNPEK
                     TVAARSLLGVLGLGVSSGHEIRISATGDDADAAITTLRELVESGLGEELAPDAPG"
     gene            4520..>5723
                     /locus_tag="VFV59_05050"
     CDS             4520..>5723
                     /locus_tag="VFV59_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU5325231.1"
                     /translation="MSRSLRGVAAAPGVALAAPWVYRPPTGGEDRLTLDEAVAVAAAE
                     LAALAARLRERGRAEEAGILDAQALMAEDPELLDAARAAVDGGAPASEAVIAAGEEQA
                     AMLAALDDEVLSARAADVRDVAERIARAIRGQSVPRLERPSVAVALDLPPSVTAELDP
                     ALLIGIALEAGSPTAHAAILARALGIPAAVGVPGLLAAAEGATAIGVVGDGGEVVIDP
                     DAEATERLRAAMVRREEQRRADATLRTAPLATADGHRVTLLANVGRPEEAAAAAEAGA
                     EGIGLFRTEFLFMGRATAPSVAAQTAAYAEAFAAFPGRPVVVRTLDIGGDKQLPYLRQ
                     APEENPFLGVRAIRLAAHEPNRTLLVDQLRAILAAAAQTTAEVWIMAPMVADLDDVAL
                     VRTLVDDAG"
BASE COUNT          706 a         2150 c         2068 g          799 t
ORIGIN      
        1 tcgcgcgcca cgctcaccgg cacgccgagc tcgcgcacgg cctgctccag ccgcttgcga
       61 ctgacgccga acgggaagac gtgcatggct cgctggttgc gggcacggtc gccgggcgtc
      121 gcgggccgct gtgctccgat gggccggtcg acgcccggct cctgcatctc gctgatcgcg
      181 cgcgccagct cctggcgcgg gtccgcgccg gccgtaccac cacccgtacc cgggccggtg
      241 gtgccgctgg tgccgccggt cagccgctcg ccgggcagcg tgcgtgcacg cccaccgccg
      301 gcgaccacgt caccgccgcc gggcagcgga cgcaggccgc cgcgctcgta tcggcctccg
      361 ggcgagcccg gtcgtccgcc gccgcgcccg aaggcgccga acccgccggt cggctcgatc
      421 ggtgcaaagg ccgggttgcc ggccgccgtc tcgctgatgc ggtagtcgta cttcgtctgc
      481 gccttcagct cgcctccgtc gtcgcggtag cgggcctccg gcgggaccat gtggccgcgc
      541 aggatggcgt cgaccgtctc cgccacgttg cggtgcacga tcacgctgtc gcgctccacg
      601 atctccacga ggacgtcgaa ggtcggcggc gccttgcgct cgagcaccgt cttctgggtc
      661 ccgcgccggc gtgcctcctc gtcgccgagg gtgacgggct ggatgccgcc gaccaggtcc
      721 gacagcgtcg ggttgagcat caggttgtcg agcgtgttgc cgtgcgcggt gcccaccagt
      781 tgcacgccgc gctcggcgat cgtccgggcc gccgcggcct cgagctccgt cccgatctcg
      841 tcgatgacga tgacctcggg catgtggttc tcgaccgcct cgatcatcac gctgtgctgg
      901 tccgtcggcg tgcggacctg catgcgccgc gcgtcgccga tcccgggatg cgggatgtcg
      961 ccatcgccgg cgatctcgtt cgaggtgtcc accaccacca cgcgcttgcc gaggtcatcg
     1021 gccaggacgc gggcgacctc ccgcagcatg gtcgtcttgc cgacgcccgg ccgcccgagg
     1081 agcagcaccg actgcccgcc ctcgacgatg tcgcggatga tgtcgatggt gccgaacacc
     1141 gcacgaccga tgcggcaggt gagccccacc accttcccgg cccggttgcg gatggcgctg
     1201 atccgatgca gcgtgcgctc gatccccgcc cggttgtcgt cgccgaagac gccgatgtgc
     1261 tcgatgacgt gggcgatatc ggcctcggtg atctcgcggt ccagcaggtc gacctcgccg
     1321 gtcgtggtgc gcgcctccgg cctgcggccc aggtccatga cgacctcgag caggtcggcg
     1381 cgatccggca gggcccgcag ccgctcgcag atctcgggcg gcagcgcctc catcagcgca
     1441 tcgaggtcgt cggtcgtctc gcgtctcgcc accgtcaacc cctcctcatc gttcatcggt
     1501 cattgcgtca cggccaccat ggccggctgg cgcacctgcg cgcgcacctc ccgtacgagg
     1561 agcgtgacgc ggccgatggg ctcgaagccc gtcgcttcag cagcgcgcgt gcctgccgac
     1621 tcgtatgttc gcaccggagc gaggatgccg gcggccagcg cgtccgtgct caggcggtcg
     1681 atgacggcgc cgacgaaggc cgcaccgtcc atcccgtcgc gcaccaggaa gcgcaggtag
     1741 tgcgggccgg agcggcagct gccgtgctgg gcgaagcccg cgacgcgctg ctcgtgcggc
     1801 agcagccagg catccacctc gccgaagtgc aggatcgggt tgagcgacga gcgcggcacg
     1861 atggcctcgt tcccggccga ctcccagtcg gacgcgccgt agccctcgat ccgcgcgatg
     1921 gccggcggcg tggcgtgggt ccacaggtcg aagaggtgcc acgcgtccgg ggctccggcg
     1981 ggctggagcg ccaggccgtc cgggtcgcgc cgcggacggc gaccgtcacg cgagacggtg
     2041 cgtcgcagcc aggagcgcgg tcctcgcacc acctccggtg gccgccacca gatctcctcc
     2101 tgtgcgtagg ccatgaaccc cgcctgcccg aacagctcga tgttgtcgcg cacgtccgcg
     2161 caggcggcgt ggaagcggcc cacgtcatgg cggacgccct cctccagcag cgcgttgagc
     2221 aggtggaagc ggacctcggc ggccatgggt ccctcggccg cgtcgagctc ggtgatgacc
     2281 cagtcgtcgc gatgcggctc ctcgatggcg cgacagctgc cggccagctc gccgtccacc
     2341 tccgccacca ggtggaccga cggggcacgc agcggcagcc acgacggcat gaggctcgac
     2401 aggctgatcc gcggcgagtc ggtggtcggc gccggaacgc ccaggctgcg ccgatgcgcc
     2461 tccggcgagg tgtggacgcg tcgcgacagg tcggccagcg cctgccggtc cgtcagccgc
     2521 gcggcgcgga tggtccgctc gacctcgatc acgccgtccg cccggcctcg ctcccgatca
     2581 ggtcgaccag gagccgatgc agccgccggt cgccggtcag ctcggggtgg aaggcggtcg
     2641 cgatgacgcg gccctggcgc accgccaccg gacggccgtc cggatcgcgg gccagcacct
     2701 cggccgcggg tccgacgtcg acgacctgcg gcgcgcggat gaagacggcg tgcagcggat
     2761 cagcgccaag ggccggcgcg tcgaggtcgg cctcgaagct gtccagctgc cggccgtagc
     2821 cgttgcgtcg tacttcgagg tccagcgccc ggaacatcgg ctcgtcaccg tcggcgatgc
     2881 gatcggccag caggatcatc ccggcgcacg tgccgagcat cggcgcgccg ccgcgcgcca
     2941 gcgcggcgat cggctcgcgc agcccgtatt cgtcgatcag ccgccgcatg gtcgtcgact
     3001 cgccgcccgg caggatcagc gcgtccaggt cgaccaggtc gcgcggcagg cgcacctcga
     3061 ccggctccgc accgacgtcg cggatggcgt cgacgtgctc ccgcaccgcc ccctgcagcg
     3121 cgaggacgcc gatgcggggc gttcggcccg cactcgtcat cggtctgagc agctcaccag
     3181 ccgcggacgg ccaggcgctc ctccggcggg atctcgcccg agctgatgcc gagcatcggc
     3241 gcgccgaggc cggcgctgac ctcggccagg atgtcggggt tgtcgtagtg cgtcgccgcc
     3301 cggacgatcg cccgcgcgta gcgctcgggg ttctcgctct tgaagatgcc cgaaccgacg
     3361 aacaccgcct cggcgccgag ctggcgcatc aggctggcat cggcgggcgt cgcgacgcca
     3421 cccgcgctga agttcgggac cggcagggag cccagctggg cggtctgctt caccacgtcc
     3481 agcggcgcgc gcagctcctt ggcggcggcg aacagctcgt cctcgcgcat cgactggagc
     3541 tggcggatgc ccttctgcac ggcgcgcatg tgccgcacgg cctcgacgat gtcgccggtt
     3601 cccgcctcgc ccttggtgcg gatcatggcc gccccctcac cgatgcgccg cagcgcctcg
     3661 cccaggtcgc ggcagccgca cacgaacgga atgtcgaacc gatgcttgtc gatgtggtgc
     3721 tcctcgtcgg cgggggtgag cacctcggac tcgtcgatga agtccacctc cagggcctgg
     3781 aggatctgcg cctccgcgaa gtgaccgatg cggcacttgg ccatgacggg gatggtgacc
     3841 gcggccttga tctcctcgat cagcttcggg tcgctcatgc gggccacgcc accgaacttc
     3901 cggatgtcgg acggcacgcg ctcgagggcc atgacagcca cggcgcccgc ctcctcggcg
     3961 atcctggcct ggtcggccgt gacgacgtcc atgatcacgc cgccggacag catgcgggcg
     4021 aggccgacct tggtggccca ggtagatcgt tcgatggtgg tcattcgtgt tccctacgcg
     4081 cagcaccgat gcgggcgcgc cgcatcgctc ggatttggcc actgattcta ctccgggcgt
     4141 gccccgcggc cgaatggcga ggccgggtgc ttcgtgcgcc ggtgggctcg tcacgcgcac
     4201 cggacgtcgc cgatactggc ggcatgcctg acgtcacgct cccggtccgc aacccctcgg
     4261 gcctccacgc ccgccccgcg gcgctgttcg tgaagaccgc cgccggcttc gccgccgacg
     4321 tgcgcgtcac gaacctcacc cgcaatccgg agaagaccgt ggccgcgcgg tcgctgctcg
     4381 gcgtgctggg cctgggtgtc tcgtcggggc acgagatccg gatcagcgcc acgggcgacg
     4441 acgccgacgc ggccatcacc acgctccgcg agctcgtcga atccggcctg ggcgaggagc
     4501 tcgcgccgga cgccccggga tgagccggag cctgcgcggc gtcgcggccg cgccgggcgt
     4561 ggccctcgcc gccccgtggg tctaccgccc gccgaccggc ggcgaggatc ggctcaccct
     4621 cgacgaagcc gtcgcggtcg cggcagcgga gctcgccgcc ctggccgccc ggctgcgcga
     4681 gcgcggccgc gcggaggagg cgggcatcct ggacgcgcag gcgctcatgg ccgaggatcc
     4741 cgagctgctg gacgccgctc gcgcggcggt ggacggcggc gcgcccgcct cggaggccgt
     4801 cattgccgcc ggggaggagc aggcggccat gctcgccgcg ctggacgacg aggtgctgtc
     4861 cgcgcgggcc gcggacgtgc gcgacgtggc cgaacgcatc gcgcgcgcca tccgtggcca
     4921 gtcggtccct cgcctcgaac gcccgagcgt tgcggtggcg ctggacctgc caccgtcggt
     4981 gacggcggag ctcgatcccg ccctgctgat cggcatcgcg ctcgaggcgg gctcgcccac
     5041 agcccacgcc gcgatcctgg cgcgcgccct cggcatcccc gccgccgtgg gcgtgccggg
     5101 gttgctggcg gccgcggagg gcgcgacggc catcggtgtc gtgggggacg gcggcgaggt
     5161 cgtgatcgac ccggacgccg aggcgacgga gcggctgcga gccgccatgg ttcgccgcga
     5221 ggagcagcga cgcgcggacg cgacgctgcg caccgctccg ctcgcgaccg ccgacggcca
     5281 tcgcgtgacg ctgctggcca acgtcggccg acccgaggag gcggccgcgg cggcggaggc
     5341 gggcgccgag ggcatcgggc tgttccgcac cgagttcctg ttcatgggcc gcgccaccgc
     5401 gccgtcggtc gccgcgcaga ccgccgccta cgccgaggcc ttcgccgcgt tcccgggacg
     5461 tcccgtggtg gtgcgcacgc tcgacatcgg tggcgacaag cagctgccgt accttcgcca
     5521 ggcgccggag gagaacccgt tcctgggcgt gcgcgccatt cgcctcgcgg cgcacgagcc
     5581 gaaccgcacc ctcctggtgg accagctgcg cgccatcctg gccgcggcgg cgcagacgac
     5641 cgccgaggtg tggatcatgg cgccgatggt cgccgacctc gacgacgtcg ccctcgttcg
     5701 cacgctggtc gacgacgcgg gcg
//
LOCUS       DASTYP010000106         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1355178, whole genome shotgun sequence.
ACCESSION   DASTYP010000106 DASTYP010000000
VERSION     DASTYP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355178"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFV59_05055"
     CDS             complement(<1..970)
                     /locus_tag="VFV59_05055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325232.1"
                     /translation="MSIPVDRSYRGLLRVPTLGRVLLSMQVARVAQSMVGVALVLFTL
                     DRYGSPAFAGLVTFASVFPGLLISPIAGALLDRHGRIRLVLLDYVVALLAMAVIGSLA
                     LADALPAPLLLAIAVVSSLTSILSHVGLRSLFPILVPEPLWERVNAVDSNGYVVATIL
                     GPPLAALGVALLGAPVTLLLIGLAFGAAAFILRRVPEPSAPTVSSGRVLADAWAGVVY
                     TWRNRTLRGLGFAITLGNLAGGMTTIVVPLIVLERLGQSEALVGFVFAASGLSGMASA
                     FLFGRMDSRNREWAMLVVPMLFMVPTVGLLLVAAGQAAPLVGLGVLV"
     gene            complement(1008..1502)
                     /locus_tag="VFV59_05060"
     CDS             complement(1008..1502)
                     /locus_tag="VFV59_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325233.1"
                     /translation="MTTSPSTNRRPWLMAYAVALAVVWIVTVLTWDVRSGSNPELSTV
                     AQLLQYLLVLAASTVIALIARLAAAAMPEDETVELFDGRLTFASDGGSGHAFWLALAV
                     GTGTMLVNIALLIAADALVGARDPGTWLAWIGGGLAVGCLLGLAGAMVASVLAMVVRR
                     RAHA"
     gene            complement(1591..2394)
                     /gene="arsM"
                     /locus_tag="VFV59_05065"
     CDS             complement(1591..2394)
                     /gene="arsM"
                     /locus_tag="VFV59_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEU5325234.1"
                     /translation="MTDQIHETVRQHYAAAALQATAGSAACCDEQGIGADLYSALERD
                     ELPDAAVLASLGCGNPIAVADLHPGERVLDLGSGGGIDVLLSARRVGPTGRAIGLDMT
                     DEMLALAQRNAEEAGATNVEFLKGQIEAIPLPADSVDVVISNCVVNLAADKAAVFAEI
                     ARVLRPGGRIGITDIVADDTLTAEQRAERGSYAGCIAGALSFAEFEAGLRGVGLADVS
                     LTPTHAVTDGMWSVIAKASKPADAAPAVSLEARELPLAATGCCGAGGCC"
     gene            complement(2405..2695)
                     /locus_tag="VFV59_05070"
     CDS             complement(2405..2695)
                     /locus_tag="VFV59_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5325235.1"
                     /translation="MKQADPDVAFLAALADPVRLSIVRQLAATADVCACDFTECCTVS
                     QPTVSHHLRVLREAGVVVSQRQGTNVIYSLAPDFEKRWAAVGASFANLVQIA"
     gene            complement(2704..2865)
                     /locus_tag="VFV59_05075"
     CDS             complement(2704..2865)
                     /locus_tag="VFV59_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325236.1"
                     /translation="MDLHFQMLAEARQAEITRDLRRRTLVATLRACRRRLLGIFPVGA
                     PCPPAATAC"
BASE COUNT          508 a         1042 c         1113 g          354 t
ORIGIN      
        1 cgacgagcac gcccaggccg accaggggcg ccgcctggcc ggcggcgacc agcagcagcc
       61 cgaccgtggg gaccatgaac agcatcggca ccacgagcat cgcccactcg cgattgcggc
      121 tgtccatccg gccgaacagg aacgccgagg ccatccccga cagcccggag gcggcgaaga
      181 cgaagccgac cagcgcctcg ctctgaccca gccgctcgag cacgatcagc ggcaccacga
      241 tcgtggtcat cccgccggcc aggttgccga gcgtgatcgc gaagccgagg ccgcgcagcg
      301 tgcggttgcg ccacgtgtag accacgccgg cccacgcatc ggcgaggacg cggccggagg
      361 agacggtcgg cgcggacggc tccggcacgc gtcgaaggat gaaggccgcc gcgccgaacg
      421 cgaggccgat gagcagcagc gtcaccggcg cgcccagcag cgccacaccc agcgcggcca
      481 gtggcgggcc caggatggtc gccaccacgt agccgttgga atcgacggcg ttgacccgct
      541 cccacagcgg ctccgggacc aggatgggga agaggctgcg cagcccgacg tggctgagga
      601 tcgaggtgag ggacgagacg accgcaatcg ccagcagcag cggggccggc agcgcatcgg
      661 ccagcgccag cgagccgatg acggccatcg caaggagcgc cacgacatag tccagcagaa
      721 ccaggcggat ccgaccgtgc cggtccaaca gcgcgccggc gatcgggctg atcaggaggc
      781 cggggaagac gctcgcgaag gtgacgaggc cggcgaaggc gggggagccg tatcggtcca
      841 gggtgaagag cacgagcgcg acgccgacca tcgactgcgc cacgcgcgcg acctgcatgc
      901 tgagcagcac gcgcccgagg gtcggcacgc gcagcaggcc acggtaggaa cggtcgacgg
      961 ggatggacac gggacgccga ttctagccgc gcgccggcgg tggccgatca ggcgtgggcc
     1021 ctccgacgga ccaccatcgc cagcaccgat gcgaccatcg cgccggccag gccgagcagg
     1081 cagccgacgg cgaggccgcc gccgatccag gcgagccacg ttcccgggtc ccgcgcgccg
     1141 acgagcgcgt ccgcggcgat caggagcgcg atgttgacga gcatggtgcc cgtcccgacc
     1201 gcgagtgcca gccagaacgc gtggccgctg cctccgtcag aggcgaaggt caacctcccg
     1261 tcgaacagct ccaccgtctc gtcctccggc atggccgcgg ccgcgaggcg agcgatcagt
     1321 gcgatcacgg ttgacgccgc gaggaccagc aggtactgga gcagctgcgc gaccgtggac
     1381 agctcgggat tcgagccgga ccggacgtcc caggtgagaa cggtgacgat ccagacgacg
     1441 gccagtgcga cggcataggc catcagccac ggacgtcggt tggtgctggg cgacgtcgtc
     1501 atgtggttcc tccccggccg gctgggcggc cgtcgcgggt cacgatccgc gtcggcggcg
     1561 tggccgtcga gggccgatgg tcacccggaa tcagcagcag ccgccggctc cgcagcagcc
     1621 cgtcgcggcc agtggcagct cccgcgcctc gagcgacacg gcaggtgcgg catccgcagg
     1681 cttggaggcc ttcgcgatga cgctccacat gccgtcggtc accgcgtggg tcggggtcag
     1741 cgacacgtct gccaacccaa ccccgcgcag gccggcctcg aactccgcga aggagagcgc
     1801 gcccgcgatg cagccggcgt aggagccgcg ctccgctcgc tgctcggcgg tgagcgtgtc
     1861 atcggccacg atgtcggtga tcccgatccg cccgcccggc cgcaggacgc gggcgatctc
     1921 ggcgaagacg gcggccttgt cggccgcgag gttcacgacg cagttgctga tgaccacgtc
     1981 gacagagtcg gcgggcagcg gaatcgcctc gatctgtccc ttgaggaact cgacgttggt
     2041 cgcgccggcc tcctccgcat tgcgctgggc gagcgcgagc atctcgtcgg tcatgtcgag
     2101 gccgatggcg cgaccggtcg ggccgacccg cctggccgac agcagcacgt cgatcccgcc
     2161 acccgagccc aggtccagca cgcgctcgcc cggatgcagg tccgcgacgg cgatcgggtt
     2221 gccgcagccg agagaggcca ggacggccgc atcgggcagc tcgtcgcgct cgagcgcgct
     2281 gtacagatcg gcgccgatgc cctgctcatc gcagcaggcg gcgcttccgg cggtggcctg
     2341 caacgccgcg gcggcgtagt gctggcgcac ggtctcgtgg atctggtcgg tcacggcgcc
     2401 ctcctcaggc gatctggacg aggttggcga agctggcgcc gacggcggcc caacgcttct
     2461 cgaagtcggg ggcaagcgag tagatgacgt tggtgccctg ccgctggctc accaccacgc
     2521 ccgcctcgcg caggacgcgg aggtggtgcg agaccgtcgg ctggctcacg gtgcagcact
     2581 cggtgaagtc gcacgcgcag acgtcggcgg tcgccgccag ctggcggacg atcgacagcc
     2641 ggaccgggtc cgccagggcg gcaaggaagg cgacgtcggg atcggcctgt ttcattggtc
     2701 ggttcagcag gcggtcgccg cgggcgggca tggcgcaccg accgggaaga tgccgagcag
     2761 gcgacggcgg caggctcgga gggtggcgac cagggtccgg cgccggaggt cgcgcgtgat
     2821 ctcggcctga cgcgcctcgg cgagcatctg gaagtgaagg tccatgcggt gatggtacgc
     2881 catgtatcga tcaatgtcaa tatagacgca caccgatatt gccgatggcc cttggcgcgg
     2941 gcagggcctg tgcaggaggc tggaacgcac accccgaccg gagaaggaga cgaaccgatg
     3001 acggcctcga ccattca
//
LOCUS       DASTYP010000107         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_881535, whole genome shotgun sequence.
ACCESSION   DASTYP010000107 DASTYP010000000
VERSION     DASTYP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881535"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /gene="argS"
                     /locus_tag="VFV59_05080"
     CDS             complement(<1..1635)
                     /gene="argS"
                     /locus_tag="VFV59_05080"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5325237.1"
                     /translation="MSLRADLTRALADARDRAIADGTLTVASEAVLPEVGLEQPANPE
                     HGDWASNVAMQLAPLARTAPMRIADAIVSHLERPPSIADVSVAPPGFLNFRLDPTWVA
                     AQVAPILEAGAAYGRHPAQQPRRINVEFVSANPTGPLHVGNARGAFVGDVLSRVLEAA
                     GHRVTREYYFNDFGTQVAMLGASVLAKRTGGELPEDGYRGAYVDDLAAELPPELLADA
                     QANPEDAAMTVGRWASETVRAGIEASMEHLGVHFDVWKEESSLHTGGWVERAVQRLRD
                     AGFVYEEEGATWFRSTAFGDDKDRVVYRSNGQPTYFASDIGYVTEKFSRGFDELIYIW
                     GADHHGTVARVRNAAEAMGFDREAVRMLLIAWVRFVRDGVEIPMSKRSGEFITLDEVL
                     AEVGVDAARWFFSSRAFTTGIDFDLELAKKQSNENPVYYVQYAHARCSSILRNAAETG
                     VKADASGTGPLLQHPSEQALLRHLLDFPHAVATAAARRETHEVPKFVYELASAFSAFY
                     RDCKVLSEDTALSAARLALVDATRSVLANGLGLLGIS"
     gene            complement(1682..1852)
                     /locus_tag="VFV59_05085"
     CDS             complement(1682..1852)
                     /locus_tag="VFV59_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325238.1"
                     /translation="MNPNTVTTTAQRPNRILVILAAAWMIVALTIGGSLTGQNPTDSS
                     STTTTVTTASRP"
     gene            complement(1984..>2376)
                     /locus_tag="VFV59_05090"
     CDS             complement(1984..>2376)
                     /locus_tag="VFV59_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HEU5325239.1"
                     /translation="VAVGARKPLIAFNVNLRTDNLALAVKIANTIRESSGGMPAVQAM
                     GVLLEPPGQAPMAQVSMNLVDWERTGIAAVVREVRRMALEAGTDIDHCELIGLAPTGA
                     LLEVAADTLGLRDFSADQALELRLARDR"
BASE COUNT          338 a          802 c          884 g          352 t
ORIGIN      
        1 ggagatgccg agcaggccca gcccgttggc gaggacgctg cgggtcgcgt cgaccagcgc
       61 cagccgcgcg gccgagaggg ccgtgtcctc gctcaggacc ttgcagtcgc ggtagaacgc
      121 ggagaaggcc gaggccagct cgtagacgaa cttcgggacc tcgtgcgtct cccgacgcgc
      181 ggcggcggtg gcgaccgcgt gagggaagtc caggaggtgg cgcaggagcg cctgctccga
      241 cgggtgctgg agcagcgggc cagtcccgga ggcatcggcc ttgacgccgg tctcggcagc
      301 gttgcgcagg atggacgagc agcgggcgtg cgcgtactgc acgtagtaga cggggttctc
      361 gttgctctgc ttcttggcca gctcgaggtc gaagtcgatc ccggtcgtga aggcgcgtga
      421 gctgaagaac cagcgagccg cgtcgacgcc gacctcggcc agcacctcgt caagggtgat
      481 gaactcgccc gagcgcttgc tcatcgggat ctcgacgccg tcccggacga agcggaccca
      541 ggcgatgaga agcatgcgga ccgcctcgcg atcgaagccc atcgcctcgg cggcgttgcg
      601 gacgcgcgcg acggtgccgt gatgatcggc gccccagatg tagatgagct cgtcgaagcc
      661 gcgactgaac ttctccgtca cgtagccgat gtcgctggcg aagtacgtcg gctggccgtt
      721 ggaccgatac accacccggt ccttgtcgtc accgaacgct gtcgaccgaa accaggtggc
      781 gccctcctcc tcgtacacga agcccgcgtc gcggaggcgc tgcacggcgc gctcgaccca
      841 gccgccggtg tggaggctgc tctcctcctt ccacacgtcg aagtgcacac ccaggtgctc
      901 catggacgcc tcgatgccgg cgcggaccgt ctcggacgcc cagcgcccca cggtcatggc
      961 ggcatcctcg gggttggcct gcgcgtcggc caggagctcg ggcggcagct cagccgccag
     1021 gtcgtcgacg taggcgccgc gatagccatc ctcgggcagc tcgccgccgg tgcgcttcgc
     1081 caggaccgat gcgccgagca tcgcgacctg ggtgccgaag tcgttgaagt agtactcgcg
     1141 cgtcacgcgg tgaccggccg cctcgaggac gcgggagagc acgtcaccca cgaaggcgcc
     1201 gcgcgcgttg ccgacgtgga gcggcccggt cgggttcgcc gagacgaact cgacgttgat
     1261 gcggcggggt tgctgcgcgg ggtggcgtcc gtacgcggca cccgcctcga ggatcggtgc
     1321 gacctgcgcc gccacccagg tcggatcgag gcggaagttc aggaatccgg gcggcgcgac
     1381 cgacacgtcg gcgatcgacg gcggccgctc caggtgcgag acgatggcgt cggcgatgcg
     1441 catcggcgcc gtgcgcgcca gcggcgccag ctgcatggcg acgttgctgg cccagtcccc
     1501 gtgctccggg ttggccggct gctcgaggcc gacctcgggg aggacggcct cggaggcgac
     1561 ggtcagggtg ccgtcggcga tggcgcggtc gcgcgcatcg gccagggcac gggtgagatc
     1621 ggcgcggagg ctcatggggc gtcgagtcta gcggtcgacg ccgggcgcgg cctcgcgcgc
     1681 gttacggacg gctggcggtc gtgaccgtcg ttgtggtcga cgagctgtcg gtcgggttct
     1741 gaccggtcag cgatccgccg atcgtcagcg cgacgatcat ccatgcggcc gccaggatca
     1801 ccaggatccg gttcgggcgc tgggcggtgg tggtgacggt gttcggattc atcggtctgg
     1861 tgggcgttct ctgatggtcc ttcgggttgg tgggcacgtc ccccgatggg cgaggaaagg
     1921 caatccgcgt gccaacgcgt gaaccaccgg tgcgccgatg caccgaagcc ccgtccccgg
     1981 acctcagcgg tcgcgcgcga gtcgcagctc gagggcctgg tccgccgaga agtcccgcag
     2041 gccgagggtg tcggccgcga cctccagcag ggcgccggtc ggcgcgaggc cgatcagctc
     2101 gcagtggtcg atgtcggtgc ccgcctccag cgccatccgc cgcacctcgc gcacgacggc
     2161 cgcgatcccc gtgcgctccc aatccaccag gttcatgctg acctgcgcca tcggcgcctg
     2221 gccgggcggc tccagcagga cgcccatggc ctgcacggcg ggcatgccgc ccgacgactc
     2281 gcggatggtg ttggcgatct tcacggccag ggccaggttg tccgtccgca ggttcacgtt
     2341 gaaggcgatg agcggcttgc gggcacccac ggcgac
//
LOCUS       DASTYP010000108        22214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1173384, whole genome shotgun sequence.
ACCESSION   DASTYP010000108 DASTYP010000000
VERSION     DASTYP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 22214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22214
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173384"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFV59_05095"
     CDS             complement(<1..887)
                     /locus_tag="VFV59_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325240.1"
                     /translation="MARVRAAGWAGGAVLALGLVAQPPSTARAADEPSLTLPGSTLTA
                     VTVDLDGDAAREVVRLRGDGPASSVLEVWAHDGATWSMTDEVATEPIRIADGSPADSQ
                     GIGLLRWTHAGGEELVLVTAATDVSGSTFPSPCCLAFSMLERAGRGYRIVGVPGGGGS
                     GDAAMVVDLDGDGTDEIVTQRTVVLSDTDPGTLELDVHRWSDGRFETMQRLSSPVAYG
                     VLPAETDGRPGDELLLYGTLDGSLVRYAFRDGAVVAEETPLGTADRGDAFLGGVADGL
                     IVVNGSQTASVVRWPADGPA"
     gene            complement(903..2054)
                     /locus_tag="VFV59_05100"
     CDS             complement(903..2054)
                     /locus_tag="VFV59_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5325241.1"
                     /translation="MDFALSPQHEEIRRTVREFAERRIAPVADELERRGEFPMEIIRE
                     AAGLGLLGVPYPEEIGGTGLDSLAYAITVEELSRVSGSVGIIVSAHTSLGCNPLWLAG
                     TDAQKEKYLRPLASGKVIGAYGLTEPGAGSDSRGTRTRARRAGECWVLNGSKRFITNA
                     GVAGTYIVTAVTEAEQSSGRISAFIVPADTPGFSVGRMEEKMGLHASNTGELLFDDCR
                     IPLENLLGAEGEGDKLFLKTLDGGRIGIAAMALGLAQAAYEAASAYAKEREQFGRPIG
                     AFQGVAFMIADMATEIDAARLMTYRAAWLKDRGRSYSTEAAMAKLFASEVAQRATNAA
                     VQVHGGYGYITEYKVERYLRDAKLTEIGEGTSQIQRMVIARNLLGLRAM"
     gene            complement(2199..2330)
                     /locus_tag="VFV59_05105"
     CDS             complement(2199..2330)
                     /locus_tag="VFV59_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325242.1"
                     /translation="MAKLNHQKRQRPRTDAARRRRRTLQQRDERRTRRDLEHWRRSS"
     gene            2487..3665
                     /locus_tag="VFV59_05110"
     CDS             2487..3665
                     /locus_tag="VFV59_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325243.1"
                     /translation="MSTSSDVDVRLDRVTKRFGEFTAVDELSLEIEHGEFFSLLGPSG
                     CGKTTTLRMIGGFEEVTAGTIYLGDADVTDLPPFKRDTNTVFQNYALFPHLNVYENIA
                     FGLRRRKVPPNEIRHQVAFMLKLVELPGYESRKPSQLSGGQQQRVALARALINHPRVL
                     LLDEPLGALDLKLRKQMQVELKRIQSEIGITFIFVTHDQEEAMTMSDRIAVMRHGHIE
                     QLGTPEELYERPRTEFVAGFLGVSNLLDAEVAGRSSDWVDLRLADGTVVRAPGADVNG
                     SSHVRIGVRPEKLRVVAIGDGATTVPVAAGETNAIEGVVLDASYIGVSTQYLVETADG
                     RKLTVYAQNLETSGASEAHPDGQRVRLTWKPQHTFVIGSAGVAAREEMPGEEEVAANV
                     "
     gene            3658..4938
                     /locus_tag="VFV59_05115"
     CDS             3658..4938
                     /locus_tag="VFV59_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5325244.1"
                     /translation="MSDQTQIERAIARAFEPRRRLSRRSFLRQGGRGVVIAGSALTIP
                     SILAACGIGPGASPSAGAGSQGALPSAPAGTLDFANWPAYIDIDEETGEYPTLAKFTD
                     ETGIQVAYTEDINDNEEFFGKIQPDLSAGNSTGYDLIVMTDWMIEKMIRLGYLEELDH
                     SKLGNFDANALDLYKDPWYDPGNVHSIAWQSGITGIGYNPTLTGRPITKFDDLLDPEF
                     AGRVGLFSEMRDTMSLTLLSLGIDPETATVEDATRAKDKLLEAAQAGQFRNFYGNDYY
                     DELANGNLALTVAWSGDVSQMKLYDNPDVEFVVPADGGMLWIDNMAIPKAAAHPLDAH
                     MMMDFWYDPANAVPLTEYVGYFSPVKGVTEKVLEDADAAEAEGDTEWADALRVISATA
                     NPTDEVLSQVHNYKKLSEDEEAQWNDLFNEVVAG"
     gene            4943..5794
                     /locus_tag="VFV59_05120"
     CDS             4943..5794
                     /locus_tag="VFV59_05120"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325245.1"
                     /translation="MPRRWRWTVPYLFLIPGGLWLLAFFVVPMLVMASVSLQEGSLGT
                     GYRMTWNFGIYPEMVQQWAAQFGRSLLYAIIVTVLTVAIGYPMAYTIAFRGGRYKSIL
                     LLLVILPFFTAYIIRTLAWKLILADNGFVLGSLKDLGILGEGFRLIATPVAVISGLTY
                     NFLPFMVLPLYVALEKIDPKLVEAATDLYASRLQAFLRVTLPLSMPGVFAGSLLTFIP
                     AVGDFINSEILGSRDTTMIGQVIQRLFLNNNAYPEAAALGFMLMAAIMGLVLVYARVV
                     GADELTR"
     gene            5794..6654
                     /locus_tag="VFV59_05125"
     CDS             5794..6654
                     /locus_tag="VFV59_05125"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325246.1"
                     /translation="MTAVAVPTAVAPVPLRRPTLRTRLARLILPVFTGLAVIYLFLPI
                     AVMIAFSFNDPAGRQNITWQGFTLRNYVDVWGRPDITGPMIASLTVAVVSTVIATVLG
                     TLIGMALTRYDFRGRSGLNLLIFIPMATPEIIMGASLLSLFVSIGQQRDLLTIVIAHV
                     MFNISYVVVTVRARIAGFNRSLEEAAMDLGADEWQTFRRVTFPLIFPGILAAALLAFA
                     LSIDDYVITSFVAGRTVTFPLWVFGVSRLGVPPEVNVLGTLIFVVALLFIAVQIWSQR
                     RALTTRPSDQ"
     gene            6651..7430
                     /locus_tag="VFV59_05130"
     CDS             6651..7430
                     /locus_tag="VFV59_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5325247.1"
                     /translation="MSYLIGEAARRVGVSPSALRLWERQGLVRPARRGRYRLYTDADL
                     EHLRGIRRLRQVDHLNAPGIRRVLRDSVATPHAENHVDGRPLRRIRERAGLSLRQASE
                     LSDLSVSFLSALERGATGASVATLQRVTSAYGTTMNELFGTTEAVEGRLVHADDRPAM
                     ELDEASVRIEQLARGATLLEPQLFVLAPGASSNGAYAHEGEEFIYLLDGAATVWVGDD
                     APYELAHPGDALTFPSTLPHRWRNDATGETRLLWINTPPTF"
     gene            7403..8713
                     /locus_tag="VFV59_05135"
     CDS             7403..8713
                     /locus_tag="VFV59_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5325248.1"
                     /translation="MDQHATDVLTDWAALRSGIPPVWARYTDLVVGRASGSWVETVDG
                     ERYLDYTCGIGVTNTGHAHPRVVAAVQEQAARALHLQQNIFHHQPGLELHRRLPQRFP
                     NGRSEVEYGLFLSNSGAEAVEAAVKLAKYVTRRPNVVAFRGGFHGRTHGAMALTSSSV
                     KYRAHYEPLMGGVHFVPFPYPLRMGGPDAALSTTLAALDDLFATLVSPEDVAAFVVEP
                     ILGEGGYVIPPDGFLPALREVADRHGILLAVDEVQTGYARTGRFFATEWTDATPDIVI
                     MAKGIASGLPLSGILAPRELLDRMGPGSHGGTYGGNVVACAAGLATLDVIEDEDLAGR
                     AHRLGERLLEGLREAAGDHPAVAEVRGRGMMVAIELAEAGTLAPRGDLAKALLAEAMA
                     RKLLLLTCGTQGQVVRIIPPLVTTDDEVELAISTIAEALGAIGH"
     gene            8735..9127
                     /locus_tag="VFV59_05140"
     CDS             8735..9127
                     /locus_tag="VFV59_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5325249.1"
                     /translation="MGQRIWVVDDEPFIRTAMLAVLEEVGHEARGFADAEGLYTALVQ
                     EGVTPDLLVLDQRLPDEFGTEILHSLRERPQYREIPVVFVTAIDDEEADRLVELAPVL
                     RKPFDFTDFVDIVRANLPAQEQPAPDAG"
     gene            complement(9137..9721)
                     /locus_tag="VFV59_05145"
     CDS             complement(9137..9721)
                     /locus_tag="VFV59_05145"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5325250.1"
                     /translation="MSEPVWERLRAPDHRRLRIGPIRRASVEQLRIVDGQLPEHDDAV
                     AFDAHHEPDGRIRVRAAARDDIDVTPWEIVADRLDIDGSDGRLEALLAGEGRVAAPGE
                     LDRQACNCRGVSVDAAYHTIAAGWETADAVKRATRIGFGPCQGRRCIPWLADRLELDP
                     EDRLAQITPRPPLVPVPISVLRAFVEPDGTRTPD"
     gene            complement(9718..10215)
                     /locus_tag="VFV59_05150"
     CDS             complement(9718..10215)
                     /locus_tag="VFV59_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5325251.1"
                     /translation="MDEEERRRLRALMGRFATGVTVIAARHGPLLSGMTANAIASISV
                     DPPLLLASIATKAETHTAVIGSHAFAVSVLADDQRELADCFAQPTTAVKLTRFCGADW
                     HEAETGSPILEGALAFFDCRLVERHVGGDHTMFLGEIVAAGYREDAEPLVWYASGYRR
                     LGADA"
     gene            complement(10226..11071)
                     /locus_tag="VFV59_05155"
     CDS             complement(10226..11071)
                     /locus_tag="VFV59_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5325252.1"
                     /translation="MRIYLSVDMEGLAGVAHAHQVSFGPAVDRVDYDRSRALMAAETN
                     AAVEAAFERGAEEVVVNDSHWQMRNLRAEDLHAGARLIVGDKPYSMTQGIGLPGDGAF
                     DGAAFIGYHAGAGNPRGVIAHTYSSATILEVRVNGVPHNEAAVNAIRLGHHGVPVMLV
                     AGDDALAEEVEQLLPWAERVVVKRALGYSVAESLSPREACTAIRDGMRRAIGRLTEMR
                     PYRPELPLHAEIDFRLPVMADHAAVLPGTERIGGRSVAFTAPDGDTFYRTFAVLFRLA
                     GIPAA"
     gene            complement(11081..11377)
                     /locus_tag="VFV59_05160"
     CDS             complement(11081..11377)
                     /locus_tag="VFV59_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325253.1"
                     /translation="MTGMRPPGTGLAAMRIGVEFGAPEDFADGMERALARGGVRGATL
                     VAALDRGDMSIHIPREDGPCWNHVPLFHLHAGEEPTAEDWATTSGILEKLERYR"
     gene            complement(11374..11793)
                     /locus_tag="VFV59_05165"
     CDS             complement(11374..11793)
                     /locus_tag="VFV59_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325254.1"
                     /translation="MQPQPTPESPEPSSVPIGRSGRFDAMALSTAGDHAVLVRDLRHD
                     NRGRALLTTDKLPSGERLVVMPLDDEVDVRVDGDALAVWLGAGAGAPLPTHEMPVEAQ
                     HVPFWATVIGGGLLLLLAAFAVIGSAVTFTWLVRIFG"
     gene            complement(11807..12742)
                     /locus_tag="VFV59_05170"
     CDS             complement(11807..12742)
                     /locus_tag="VFV59_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5325255.1"
                     /translation="MTRPLKVGVQLPEVERVVRWPELREMTLTAERIGLDSIWVGDHL
                     LYRDDGLPPRGPWEAWSTLAAIGAITERVEIGPLVAATSFHNPAMLAKKAATVDEISG
                     GRLILGVGAGWNEAEYRAYGFPFDHRVSRFEEAFTVIRTLLATGRCDFHGTYYDLEDA
                     ELLPRGPRPEGPPLMVGSMGERMLGITLPHVSAWNAWFTWFGNSVEGYRELHERIDQG
                     CRTAGRDPQDVERTVALFVGFPGAQGRQLGDLNRPDVEHLPGDAAQLAATLADFAAAG
                     VAHVQLVLDPITVDTIAALEPMLAVLDGRGAVRGG"
     gene            complement(12769..13674)
                     /locus_tag="VFV59_05175"
     CDS             complement(12769..13674)
                     /locus_tag="VFV59_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5325256.1"
                     /translation="MPEWDPERVVDPDLARALIEARVPELRGARIERLAEGWDNVVYV
                     VDGRWVFRFPQREIAVPGVRREIETLERIGPHLPLPVPVPRWVGDPDERYPWPWFGTE
                     HLPGVELASSGLPDERRATIGRELGRFLRALHGPMLRSRIGPTLPIDPMRRTDMAHRV
                     RLTRERLARVVGAGLWEPTAAVDALLRDARDLPPSPRVVVLHGDLHVRHVLVDGERPT
                     GVIDWGDVCAGDPSADLSLAYGALVGAAREAFLVAYGPVDGLTELRARVIAVFLAAAL
                     LEYAADAGMAALGAEASDALARAVA"
     gene            complement(13753..15786)
                     /locus_tag="VFV59_05180"
     CDS             complement(13753..15786)
                     /locus_tag="VFV59_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5325257.1"
                     /translation="MTTFRYSRWDGSQQLPAFDADDVLDALSDDILAEGDVRRALQRL
                     MQRGLRGTRGGDIPGLRRILERLRAQRQEELERSNLDGVLEDLNQRLEEILKQERRGI
                     EERTKAAEQRALDAPPGPEQDQARMAEQVVRRTARQRENRLDALPPNLAGRLHGLRDY
                     EFMDDGAREAFNALTDELRQQMLQTYFQGLKEGVERITPEDLAGVREMVRDLNQLLEK
                     HATGADTTEDFAAFMAKHGSYFPPGISDTDALIEHLHRQASQMASLLNSMSPQMREEL
                     RDLMDDLLRDDRLKIDMARLAGNLQAARPDLPFGEPYPFAGDEPMGLADALAAIDRQQ
                     QYDAMEEALGAARDPDGLEKVDAEALRDLAGDDAIDDLEQLRELTKALEEAGYLDRDG
                     GRLELTPRAARKLGMRALTDIFSRLRREGFGGHPLPAAGRGGEPTEETKPYEFGDPFA
                     LDINRTLFNAMARGGPGTPVSIAPADFEVARTEETTTSSTVLLLDMSRSMLLRGCSSA
                     AKRVAMALHTLIHTKYPRDRLYVVGFAYYARQLKPESIPTLSPYEFEYGTNLQHALII
                     ARQLLGRSTGGNKEMVVITDGEPTAHIEGGQVEFAYPPTLRTVQSTLREVGRCTREGI
                     VINTFMLERSRYLSDFVDLMSRINRGRAFYVEPEHLGEYVLVDYVSKKTKRVA"
     gene            complement(15843..16400)
                     /locus_tag="VFV59_05185"
     CDS             complement(15843..16400)
                     /locus_tag="VFV59_05185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325258.1"
                     /translation="MPAQDLRLRLAEPDRDAEAIASIYRPAVDSTIASFEEAAPTADE
                     MARRTSATLERFPWLVAELDANVVGYAYAGPHRDRPGYRWSANVSVYVDAAWHGQGIG
                     RALYDGLFDLLRRQGYVNAYAGIALPNPASVALHAAMGMRQVARYERVGWKFGAWHDV
                     AWYWLRLAEPEGAPPEPVPIASLDR"
     gene            16399..16800
                     /locus_tag="VFV59_05190"
     CDS             16399..16800
                     /locus_tag="VFV59_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325259.1"
                     /translation="MAGIVRDAARGVLARASMSSNDIHGIESGWSVKTADGEGIGSVE
                     ETTDRYILVKSGLINASRHYLPAVELEHVRPEQKEIGVSLTKAEFEAGDWSEPPTQGP
                     RADVPLNPDTARDEDNPIRHQPVDPERPISL"
     gene            16856..17527
                     /locus_tag="VFV59_05195"
     CDS             16856..17527
                     /locus_tag="VFV59_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325260.1"
                     /translation="MTRRALFLVLPFALAVPAAYGLAFAYAGYPLELGPLLIGALGWV
                     LALVLRAPVGLAGLKITGSQESAQRWVVASSGPLEESVRLAALLLVGRDLPTALWLGL
                     GWAAIEVGYAIANGFAMAALASRTDPEAERARAMLPPSAFAASAPWWGVVERIWASAL
                     HIGFTLMLAAAPILWVVTVPLHTLTNVTFVRLGQRRSMLIVSGLGLLFGAIILAGGLA
                     LHRIG"
     gene            17608..21177
                     /locus_tag="VFV59_05200"
     CDS             17608..21177
                     /locus_tag="VFV59_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5325261.1"
                     /translation="MICTACGAENDAGRKFCLQCGQRLAVACPSCGTANPAGARFCGE
                     CGGPLGGADAIAGVPAVSSGASTGPAQSRTTTATERRLVSVLFADLVGFTTISESRDA
                     EDVRALLDRYFATSREIVERYGGIVEKFIGDAVMAVWGTPVAQEDDAERAVRAALDLV
                     DAARALGQQAGVPDLALRAGVLTGEAVVTIGATGMGMVAGDVVNTASRLQSVSAPGSV
                     LVGEATRRAASEAIVFEPAGEQTLKGKESPVPAFRALRVVAQRGGVGRSELLEPPFVG
                     RDPELRLIKDFYHATAREQGPRLVSVIGQAGIGKSRLAWEFLKYIDGVTENVWWHEGR
                     SPAYGDGISFWALGEMVRMRAGIGEGADAGTTRERISASLTEFVVDPDERASLEGPLL
                     HLLGISDETAFERSALFVAWRTFFERIADQGPVVMVFEDLQWADDGLLDFIEDLVTWS
                     RGRSIYVITLARPELLDRRPTWGAGLRAFTSLSLQPLSDEDVRTLLDGLVPGLPSNVT
                     EAIVARAEGVPLYAVETVRMLLNDGRIERDGDRFRPVGDLSALAVPESLHALIAARID
                     ALPAAERALLQDASVLGQSFSREALVAVTRVDEGEVDGMLRHLAQRELIDVDDDPRSP
                     ERGQYRFVQGLLREVAYGTLARDDRRTRHLAAARYYETLGDDERAGVLAQHYVDAYQA
                     HPDGPEGAAVAAQARVALRAASQRAAALGSHRRALQYIESALSVTTDQREELDLRVAA
                     GTRAGDAGLMEPAVTHLERAIDIARLLGEHSIRRRATTTLADVLTEGRQKESQQLLEQ
                     LLAEPDFDESDPEYLNAATQLAKVHMRRLEDLKAIELADRMLPLVERGGDQELALDLL
                     ITRGVALGNLNRNVEATALIQGALESALRGGHVDVALRGYTNLGYVLASDDVQAAHRV
                     SREGVEMAIKTGKTWPIRYLVGNAIDGALEIGEWDWLLRTVAEQDQGELELAERIWYS
                     AYRLLVETFRGEEVEHEAGEIYEASRTVDDLQYLLIGSYPMTAWALVNGHYDRVMQIS
                     DGLLGQGPINAAEVAAWAARAAAWAGDVEALRRYAEASRATRSGRRSEVLLHATSGQL
                     AALEGRDADARAAFAAALRLSRDLGLRFWTGLIALDVLTLGAMEPDERARAADEARVI
                     FTDLGATPLLRRLDQVGPPTSQRGGSARGQEAPTEAAEVPGEA"
     gene            21174..>22214
                     /locus_tag="VFV59_05205"
     CDS             21174..>22214
                     /locus_tag="VFV59_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5325262.1"
                     /translation="MTLAQRAFIAATRLGARPEDSAVDRLQRGLLVSVLVIVIGPCLA
                     WGALYWLNGERVAALVPWTYIGLCVASLAVLATTGAFRLVRSVQLTLILVLPMTLCVL
                     LGGIVPSSGVVLWSFLAPVAAIAFDRPDRAWRWFAGFIGVLLLAIVLPPFVRPEPAAM
                     PDAMVLAFAGLNIGAVGLLCFSLLAAFATERESAQRRVEELLLNVLPRGIVERLQTER
                     GSIADHFDQASVLFADVVDFTPLSQRLPPREVVDMLDRLFTEFDALADALGVEKIKTI
                     GDCYMAASGVPVSRADHAVAIARLALSMRECVGRFPRADDGRPLRIRIGLNAGPVVAG
                     VIGRRRFLYDLWG"
BASE COUNT         3126 a         7920 c         7883 g         3285 t
ORIGIN      
        1 gccggtccgt cagcgggcca gcgaacgacg gacgcggtct ggctcccatt cacgacgatc
       61 agcccgtccg cgacgcctcc caggaaggcg tcgccgcggt ccgcggtacc gagcggggtc
      121 tcttccgcca ccacggctcc atctcggaac gcgtatcgca cgagggaacc gtcgagggtg
      181 ccgtacagca gcagctcgtc gcccggccga ccgtcggtct ccgcggggag aaccccgtag
      241 gcaacggggc tggagaggcg ctgcatcgtc tcgaatcggc cgtcgctcca gcgatggacg
      301 tcgagctcga gcgtgcccgg atcggtgtcg gacagcacca ccgtgcgttg cgtgacgatc
      361 tcgtcggtgc catcgccatc gaggtcgacg accatggcag cgtcacccga ccccccgccg
      421 cccggcacgc cgacgatccg gtatccccgg ccagcccgct ccagcatcga gaacgccagg
      481 cagcagggcg acgggaacgt tgacccgctg acatccgtcg ccgccgtgac gagcaccagc
      541 tcctccccgc cagcgtgcgt ccagcgcaac agtccgatgc cctgcgagtc cgccggcgat
      601 ccgtcggcga tccggatcgg ctccgtcgcg acctcgtcgg tcatcgacca cgtggcgccg
      661 tcgtgtgccc aaacctcgag gaccgatgag gcgggcccat caccgcgcag ccggacgacc
      721 tcgcgcgcgg catccccgtc caggtccacg gtcacggcag tcagggtcga ccccggcagg
      781 gtcagtgacg gctcgtccgc ggcgcgcgcc gtggatggcg gctgcgcgac gaggcccagc
      841 gcgaggacgg cgccccctgc ccagccggct gcgcgcaccc gcgccatggg tcgtccgtgc
      901 gcctacatcg cacgcaggcc gaggaggttg cgcgcgatca ccatgcgctg gatctggctg
      961 gtcccctccc cgatctcggt cagcttcgcg tcgcgcaggt agcgctcgac cttgtactcg
     1021 gtgatgtagc cgtagccgcc gtggacctgg accgccgcgt tcgtggcgcg ctgcgcgacc
     1081 tcggaggcga atagcttcgc catggccgcc tcggtcgagt agctgcggcc ccgatccttg
     1141 agccaggccg cgcggtaggt catcaggcgc gccgcgtcga tctcggtcgc catgtcggcg
     1201 atcatgaagg cgacgccctg gaaggcgccg atcggccgcc cgaactgctc gcgctccttc
     1261 gcataggccg atgcggcctc gtaggccgcc tgcgccaggc cgagcgccat ggcggcgatg
     1321 ccgatgcgcc ccccatcgag cgtcttgagg aacagcttgt cgccctcgcc ctccgcgccg
     1381 agcaggttct cgagcgggat gcggcagtcg tcgaagagca gctccccggt gttgctggca
     1441 tggaggccca tcttctcctc catccgcccg acgctgaagc ccggcgtgtc ggccggcacg
     1501 atgaaggcgc tgatccggcc gctgctctgt tcagcctccg tcaccgccgt gacgatgtag
     1561 gtcccggcca cgcccgcgtt ggtgatgaac cgcttcgagc cgttcaggac ccagcactcg
     1621 cccgcgcgtc gcgcacgcgt ccgcgtgccg cgggagtcgc tccccgcacc gggctccgtc
     1681 agcccgtagg cgccgatcac cttgccggac gccagcggtc gaaggtactt ctctttctgc
     1741 gcatcggtcc cggcgagcca cagcgggttg cagcccaggc tggtgtgggc gctgacgatg
     1801 atcccgacgc tgccactgac gcgactgagc tcctcgacgg tgatggcgta ggcgagcgag
     1861 tccaggccgg tgccgccgat ctcctcggga tacgggacgc cgaggaggcc gaggccggcc
     1921 gcctcgcgga tgatctccat cgggaactcg ccacggcgct cgagctcgtc ggcgaccggg
     1981 gcgattcgcc gctcggcgaa ctcgcggacg gtgcgacgga tctcctcgtg ctgcggggag
     2041 agggcgaagt ccatgtgggg cggcgctcca gctgcggaat cggtggcgct cgaggagtgc
     2101 gagtctacca gcgcccaccg gcgatccggt tcgccacacc cggacatgga tggaccgccg
     2161 atcgatcgat cagcggtccg gtgccatgcg ggcgcgaatc aggaggagcg gcgccagtgc
     2221 tccaggtctc ggcgcgtgcg gcgttcgtcg cgctgctgaa gggtgcggcg gcggcggcgt
     2281 gccgcatcgg tgcgtgggcg ctgccgcttc tggtggttga gcttggccat ggtgcggtgt
     2341 tgtgctcccg tgtcggtaac cccaatgacg cgcagaaccg gctcgaagtt gcagccagca
     2401 cgacattcgc ttgctctgtt gacgggcgag aggagggccg ccgtatactc cgcgggctta
     2461 ctgcaacgcg acaaggttgg gcccgggtga gcacttcgtc cgacgtcgac gttcggctcg
     2521 atcgcgtgac caaacggttc ggcgagttca ccgcggttga cgagctgagc ctggagatcg
     2581 agcacggcga gttcttctcg ctgctcggcc cgtcgggctg cggcaagacc accacgctgc
     2641 ggatgatcgg cggcttcgag gaggtcaccg ccggcacgat ctacctgggt gacgcggacg
     2701 tcaccgacct gccgccgttc aagcgcgaca cgaacaccgt cttccagaac tacgcgctgt
     2761 tcccgcacct gaacgtctac gagaacatcg ccttcggcct gcgccgtcgc aaggtgccgc
     2821 cgaacgagat ccggcaccag gtggcgttca tgctcaagct ggtcgagctg cctggctacg
     2881 agtcgcgcaa gcccagccag ctgtccggcg ggcagcagca gcgggtcgcg ctggcgcggg
     2941 cgctcatcaa ccaccctcgc gtcctcctgc tggacgagcc gctcggcgcg ctcgacctca
     3001 agctgcgcaa gcagatgcag gtcgagctga agcgcatcca gagcgagatc ggcatcacct
     3061 tcatcttcgt cacccacgac caggaggagg cgatgacgat gtcggatcgc atcgccgtga
     3121 tgcggcacgg gcacatcgag cagctcggga cgcccgagga gctgtacgag cggccgcgga
     3181 cggagttcgt ggccggcttc ctgggcgtga gcaacctgct cgacgccgag gttgccggac
     3241 gctccagcga ctgggtcgac ctgcgactgg cggacggcac ggtggtgcgc gcaccgggtg
     3301 ccgacgtgaa tggctcgagc catgtccgga tcggagtccg tcccgagaag ctgcgggtcg
     3361 tggccatcgg cgatggcgcg acgacggtgc cggtcgcggc cggggagaca aacgccatcg
     3421 agggcgtggt gctcgacgcc tcgtacatcg gcgtcagcac gcagtacctg gtcgagacgg
     3481 cggacggccg gaagctgacg gtctacgcgc agaacctgga gaccagtggc gccagcgagg
     3541 cgcacccgga tgggcagcgg gtgcggctga cctggaagcc acagcacacg ttcgtgatcg
     3601 ggtcggccgg cgtggccgcc cgcgaggaga tgcccggcga ggaggaggtt gcggccaatg
     3661 tctgaccaga cccagatcga gcgcgccatc gcgcgtgcct tcgagccacg ccgccggttg
     3721 agccggcgat ccttcctgcg gcagggcggc cgcggcgtgg tcatcgccgg ttccgcgctg
     3781 accatcccgt cgatcctggc cgcgtgcggc atcggtccgg gggcgtcacc gagcgccggg
     3841 gcggggagcc agggggcgct gccgtccgcg ccggcgggca cgctcgactt cgcgaactgg
     3901 cccgcgtaca tcgacatcga cgaggagacc ggcgagtacc cgacgctggc caagttcacc
     3961 gacgagaccg ggatccaggt cgcctacacc gaggacatca acgacaacga ggagttcttc
     4021 gggaagatcc agcccgacct gtcggccgga aactcgaccg ggtacgacct catcgtcatg
     4081 accgactgga tgatcgagaa gatgatccgg ctcggctacc tcgaggagct cgaccactcc
     4141 aagctgggga acttcgacgc caacgccctc gacctgtaca aggacccgtg gtacgacccg
     4201 ggcaacgtcc acagcatcgc ctggcagtcg ggcatcaccg gcatcggcta caacccgacg
     4261 ctgaccgggc gacccatcac caaattcgac gacctgctcg accccgagtt cgcgggccgc
     4321 gtcgggctct tcagcgagat gcgcgacacg atgtcgctca cgctgctcag cctggggatc
     4381 gatccggaga ccgcgacggt cgaggatgcg acgcgcgcga aggacaagct gctggaggcc
     4441 gcccaggccg gtcagttccg caacttctac ggcaacgact actacgacga gctggccaac
     4501 ggcaacctgg ccctgacggt ggcgtggtcg ggtgacgtca gccagatgaa gctgtacgac
     4561 aaccccgatg tcgaattcgt ggttccggcc gacggcggga tgctgtggat cgacaacatg
     4621 gccatcccga aggccgcggc gcacccgctg gacgcccaca tgatgatgga cttctggtac
     4681 gacccggcca acgcggtgcc gctgaccgag tacgtcggtt acttcagccc cgtcaagggc
     4741 gtcaccgaga aggtcctgga ggacgccgac gcggccgagg cggagggcga cacggaatgg
     4801 gccgacgcgc tgcgcgtcat ctcggccacc gcgaacccga cggacgaggt cctgagccag
     4861 gttcacaact acaagaagct gagcgaggac gaggaggcgc agtggaacga cctcttcaac
     4921 gaggtcgtcg ccggatagcg ccatgccgcg tcgctggcgc tggaccgtcc cctacctctt
     4981 cctgatcccg ggcgggctct ggctgctcgc cttcttcgtg gtgccgatgc tggtcatggc
     5041 atcggtcagc ctgcaggagg gctcgctcgg gaccggatac cgcatgacgt ggaacttcgg
     5101 gatctacccg gagatggtgc agcagtgggc ggcacagttc ggacggtcgc tgctgtacgc
     5161 gatcatcgtc acggtgctca cggtcgccat cggctacccg atggcctaca ccatcgcctt
     5221 ccgcggtggc cgctacaaga gcatcctgct gctcctcgtc atcctcccct tcttcaccgc
     5281 ctacatcatc cgcacgctcg cctggaagct gatcctggcc gacaacggct tcgtgctcgg
     5341 gtcactcaag gacctcggca tcctgggcga gggcttccgg ctgatcgcca cgccggtcgc
     5401 ggtgatcagc ggcctgacct acaacttcct gccgttcatg gtcctgccgc tgtacgtggc
     5461 gctcgagaag atcgacccca agctggtgga ggcggcgacc gacctctacg ccagccgcct
     5521 ccaggccttc ctgcgcgtca cgttgccgct ctcgatgccc ggcgtcttcg ccggctcgct
     5581 gctgaccttc attcccgccg tcggcgactt catcaactcc gagatcctgg gcagtcgcga
     5641 cacgacgatg atcggccagg tcatccagcg gctcttcctc aacaacaacg cctaccccga
     5701 ggcggcggca ctcggcttca tgctgatggc cgccatcatg ggcctggtcc tggtctacgc
     5761 ccgcgtcgtg ggcgccgatg agctgacgcg ctgatgaccg ccgtcgccgt cccgaccgcc
     5821 gtcgcgccgg tgcccctccg ccgccccacg ctgaggacgc gcctggcgcg actcatcctg
     5881 ccggtcttca ccggcctggc ggtgatctac ctcttcctgc ccatcgcggt gatgatcgcg
     5941 ttcagcttca acgacccggc cgggcggcag aacatcacct ggcagggctt cacgctccgc
     6001 aactacgtcg acgtctgggg tcggccggac atcaccggac cgatgatcgc cagcctcacg
     6061 gtggcagtgg tgtccacggt gatcgccacg gtgctcggca cgctgatcgg gatggcgctc
     6121 acgcgctacg acttccgcgg ccgctccggg ctgaacctgc tcatcttcat ccccatggcc
     6181 acgcccgaga tcatcatggg cgcctcgctg ctgagcctct tcgtcagcat cgggcagcag
     6241 cgggacctgc tcaccatcgt catcgcccac gtcatgttca acatcagcta cgtggtggtg
     6301 acggtgcgcg cccggatcgc cggcttcaac cgctccctgg aggaggcggc catggacctg
     6361 ggggcggacg agtggcagac cttccggcgc gtcaccttcc cgctcatctt cccgggcatc
     6421 ctggcggcgg ccctgctggc cttcgcgctg tcgatcgacg actacgtgat cacctccttc
     6481 gtcgccggcc ggaccgtcac cttcccgctg tgggtgttcg gcgtgtcgcg cctgggcgtg
     6541 ccgccggagg tgaacgtgct gggcaccctg atcttcgtcg tcgcgctgct gttcatcgcg
     6601 gtccagatct ggagccagcg tcgcgcgctg accacccgac cgagcgacca gtgagctacc
     6661 tcatcgggga ggccgcgcgc cgcgtgggcg tctccccctc cgcgctgcgc ctctgggagc
     6721 ggcagggcct ggtgcgtccg gcgcgacgag gccgctaccg cctctacacc gatgccgacc
     6781 tggagcacct gcgtggcatc cgtcgcctcc ggcaggtcga ccacctgaac gcccctggca
     6841 tccgccgcgt gctgcgcgac tcggtcgcca cgccgcacgc ggagaaccac gtcgatgggc
     6901 ggccgctgcg gcgcattcgc gagcgcgccg gcctgagcct gcgccaggcg tcggagctca
     6961 gcgacctgtc ggtcagcttc ctctcggccc tggagcgcgg cgcgaccggg gcaagcgtgg
     7021 cgacactgca acgcgtcacc tcggcctacg gcacgaccat gaacgagctg ttcgggacga
     7081 ccgaggccgt cgaaggacgg ctggtgcacg ccgatgaccg gccggcgatg gagctggacg
     7141 aggcgtcggt gcgcatcgag cagctggctc gcggtgccac cctgctggag ccgcagctct
     7201 tcgtcctggc acccggggcc agcagcaacg gcgcctacgc ccacgagggg gaggagttca
     7261 tctacctgct ggacggcgcc gcgaccgtgt gggtcggcga cgacgcgccg tacgagctgg
     7321 cgcatccggg cgacgccctg accttcccgt caaccctgcc gcatcggtgg cgcaacgacg
     7381 ccacgggaga gactcgactg ctatggatca acacgccacc gacgttctga ccgactgggc
     7441 ggccctccgt tccggcatcc cgcccgtctg ggctcgctac accgacctgg tggtcgggcg
     7501 cgccagcggc tcgtgggtgg agaccgtcga cggcgagcgc tacctcgact acacctgcgg
     7561 catcggggtg accaacacgg ggcacgccca tccgcgggtc gtggccgcgg tccaggagca
     7621 ggccgctcgc gcgctgcacc tccagcagaa catcttccac caccagcccg gcctggagct
     7681 gcaccggcgc ctgccgcagc gcttcccgaa cggccgttcc gaggtcgagt acgggctctt
     7741 cctctcgaac tcgggcgcgg aggcggtcga ggccgccgtc aagctggcca agtacgtgac
     7801 gcggcgaccg aacgtggtgg cgttccgcgg cggcttccac ggtcggaccc acggcgccat
     7861 ggcgctgacc agctcgagcg tgaagtaccg cgcccactac gagccgctga tgggtggcgt
     7921 gcacttcgtg ccgttcccct acccgctgcg catgggcggc cccgatgcgg ccctgtccac
     7981 cacgctcgcg gcgctggacg atctgttcgc cacgctggtg tcacccgagg acgtggcggc
     8041 gttcgtggtg gagccgatcc tcggcgaagg cggctacgtg attccgccgg acggcttcct
     8101 gcccgcgttg cgggaggtgg ccgaccgcca cggaatcctg ctcgccgtcg acgaggtgca
     8161 gaccggctac gcccgcacgg ggcgcttctt cgccaccgag tggaccgatg cgacgccgga
     8221 catcgtgatc atggccaagg gcatcgcctc ggggctgccg ctgtccggca tcctggcgcc
     8281 acgcgagctg ctggaccgga tggggcctgg ctcgcacggc gggacctacg gcgggaacgt
     8341 cgtggcctgc gcggccgggt tggcgaccct cgacgtcatc gaggacgagg acctggccgg
     8401 gcgagcgcat cggctgggcg aacgcctgct ggaaggcctt cgtgaggccg cgggcgacca
     8461 tccggccgtg gcggaggtgc ggggtcgcgg catgatggtc gccatcgagc tcgccgaagc
     8521 cggtacgctg gcgccgcgcg gcgacctggc caaggccctg ctggcggagg cgatggcaag
     8581 gaagctgctg ctgctgacgt gcggcacgca ggggcaggtg gtccggatca tcccgccgct
     8641 cgtgaccacc gacgacgagg tggagctggc gatctcgacc atcgcggagg cgctgggcgc
     8701 gatcggtcac tgaagcgagg agcgagggac gacgatgggg cagcggatct gggtcgtcga
     8761 cgacgagccg ttcatccgga cggccatgct ggccgtgctc gaggaggtcg gccacgaggc
     8821 gcgcgggttc gcggacgccg aaggcctgta caccgccctc gtccaggagg gcgtcacccc
     8881 cgacctgctg gtcctggacc agcgcctgcc ggacgagttc ggcacggaga tcctgcactc
     8941 gctccgcgag cggccgcagt accgcgagat cccggtggtg ttcgtcaccg ccatcgatga
     9001 cgaggaggcg gaccgcctgg tcgagctcgc gccggtcctg cgcaagccgt tcgacttcac
     9061 cgacttcgtc gacatcgtgc gcgccaacct cccggcgcag gagcagccgg ctcccgacgc
     9121 cggctgagac cgagcgtcag tccggggtcc gcgtgccgtc gggttcgacg aaggcgcgga
     9181 gcaccgagat cggcaccggt accaggggcg gccgcggggt gatctgcgcc agacggtcct
     9241 ccggatcgag ctccaggcgg tcggccagcc acggaatgca ccgccggccc tggcacggac
     9301 cgaagccgat gcgcgtcgcg cgcttcaccg catcggccgt ctcccagccg gccgcgatgg
     9361 tgtggtaggc ggcgtcgacg ctcacgccac ggcagttgca cgcctggcgg tccagctcgc
     9421 cgggcgccgc cacgcggccc tcgccggcca gcagcgcttc cagccggccg tcggagccgt
     9481 cgatgtccag ccggtcggcc acgatctccc acggggtgac gtcgatgtcg tcgcgcgcgg
     9541 cggcgcgcac gcggatgcga ccgtcgggtt catggtgggc gtcgaacgcc acggcgtcgt
     9601 cgtgctccgg cagctgcccg tccacgatgc gcagctgctc gacgctggcg cggcggatcg
     9661 ggccgatgcg caggcggcgg tgatcgggag cccgcagccg ctcccagacg ggctcgctca
     9721 cgcatccgcc cccagccggc ggtagccgga cgcgtaccag acgagcggct ccgcgtcctc
     9781 gcggtacccg gccgccacga tctcgccaag gaacatggtg tggtcgccgc cgacgtgacg
     9841 ctccaccagc cggcaatcga agaaggcgag cgccccctcc aggatcggcg agccggtctc
     9901 agcctcatgc cagtccgcgc cgcagaaccg cgtcagcttg accgcggtgg tgggctgggc
     9961 gaagcagtcg gccagctcgc gctgatcatc ggccaggacg ctgaccgcga acgcgtgcga
    10021 gccgatgacg gcggtgtgcg tctccgcctt ggtggcgatg ctggccagca ggagcggcgg
    10081 atccacgctg atgctggcga tggcgttggc cgtcatcccc gacaggagcg gtccgtggcg
    10141 cgccgcgatg acggtcacgc cggtggcgaa ccggcccatc aaggcgcgca gccgtcggcg
    10201 ctcctcctcg tccatccgtc gcgacctacg cggccgggat gccggccagg cggaagagca
    10261 cggcgaaggt tcggtagaag gtgtcgccgt cgggagcggt gaaggcgacg ctgcgccccc
    10321 cgatccgttc cgtgccgggc aggacggcgg cgtggtccgc catcaccggc agacggaagt
    10381 cgatctccgc gtgcagcggc agctccggcc ggtacgggcg catctcggtc aggcggccga
    10441 tcgcgcggcg catgccgtcg cggatggccg tgcacgcctc gcgcggcgag agcgactccg
    10501 cgacggagta gccgagtgcg cgcttcacca cgacgcgctc cgcccatggc agcagctgct
    10561 cgacttcctc ggccagcgcg tcgtctccgg cgaccagcat gaccggcacg ccgtggtggc
    10621 cgagccggat ggcgttgacg gcggcctcgt tgtgcgggac gccgttgacc cggacctcga
    10681 ggatggtggc cgaggaatag gtgtgtgcga tcacgccgcg cgggttaccg gcgccggcgt
    10741 ggtagccgat gaaggcggcg ccgtcgaagg cgccatcgcc cggcagcccg atgccctggg
    10801 tcatgctgta cggcttgtca ccgacgatca ggcgagcgcc ggcgtgcaga tcctccgcgc
    10861 gcaggttccg catctgccag tggctgtcgt tgacgacgac ctcctcggcg ccgcgctcga
    10921 acgccgcctc gaccgccgcg ttcgtctccg ccgccatcag cgcgcggctg cggtcgtagt
    10981 cgacccggtc gaccgcgggg ccgaagctca cctggtgggc atgggccacg cccgcgaggc
    11041 cctccatgtc gaccgagagg tagatccgca tgcgccgatg ctaccggtag cgctcgagct
    11101 tctcgaggat gccgctcgtc gtcgcccagt cctcggcggt cggctcctcc ccggcgtgga
    11161 ggtggaacag cggcacgtgg ttccagcacg ggccgtcttc gcgcgggatg tggatgctca
    11221 tgtcgccgcg gtccagcgcg gcgaccagcg tcgcgccgcg aacgcccccg cgcgccaggg
    11281 cgcgctccat gccgtcggcg aagtcctcgg gtgcgccgaa ctcgacgccg atgcgcatgg
    11341 cggcgagccc cgtccccggc ggacgcatgc cggtcacccg aagatcctca ccagccacgt
    11401 gaacgtcacc gccgagccga tgaccgcgaa ggcggccagc agcaggagca gcccgccgcc
    11461 gatgacggtg gcccagaacg ggacgtgctg ggcctcgacc ggcatctcat gcgtcggcag
    11521 gggcgcgccg gccccggcac cgagccacac cgccagggcg tcgccgtcga cgcggacgtc
    11581 gacctcgtca tccaggggca tgacgaccag gcgctcgccg gagggcagct tgtcggtcgt
    11641 caacagggcg cggccgcggt tgtcgtgccg aaggtcgcgg acgagcaccg cgtggtcgcc
    11701 ggccgtgctc agcgccatcg cgtcgaatcg accgctacgg ccgatcggga ccgacgacgg
    11761 ctccggcgac tcgggcgtgg gctgcggctg cacgccggcg atggtatcag ccaccccgca
    11821 cggcaccgcg tccgtcgagc acggcgagca tgggctccag ggcggcgatg gtgtcgacgg
    11881 tgatcgggtc cagcaccagc tggacgtgcg caacgccggc cgcggcgaag tcggccagcg
    11941 tggccgccag ctgtgccgcg tcgccgggca ggtgctcgac gtcggggcgg ttcaggtcgc
    12001 cgagctgccg gccctgcgcg ccggggaatc cgacgaacag ggcaaccgtc cgctcgacgt
    12061 cctgcgggtc gcgaccggcc gtccggcacc cctggtcgat ccgctcgtgg agttcccggt
    12121 agccctcgac gctgttgccg aaccaggtaa accacgcgtt ccaggcgctg acgtgcggca
    12181 gcgtgatgcc cagcatccgc tcccccatcg acccgaccat caagggcggc ccctccggcc
    12241 gcgggccgcg cggtagcagc tccgcgtcct cgaggtcgta gtaggtgccg tggaagtcgc
    12301 agcggccggt ggccaggagc gtgcggatga cggtgaacgc ctcctcgaag cggctcaccc
    12361 gatggtcgaa gggaaagccg taggcgcggt actccgcctc gttccatccg gctccaacgc
    12421 ccaggatcag ccgcccgccg ctgatctcgt cgacggtcgc ggccttcttg gccagcatcg
    12481 ccgggttatg gaagctggtc gccgccacca gcggcccgat ctccacccgc tcggtgatcg
    12541 cgccgatcgc ggcgagcgtc gaccacgcct cccacgggcc gcgcggcggc agcccatcgt
    12601 cgcggtagag caggtggtcc ccgacccaga tcgagtcgag gccgatgcgc tcggccgtca
    12661 gggtcatctc ccgcagctcc ggccagcgca ccacgcgctc gacctcgggc agctgcacgc
    12721 ccaccttcag cggtctggtc atcgcgctga ttgtggcagc ggcggcggtc aggcgacggc
    12781 gcgcgccagc gcgtccgacg cttcggcacc cagcgcggcc atgcccgcgt ccgcggcata
    12841 ctcgagaagc gcggcggcca ggaagacggc gatcacgcgc gcccgaagct ccgtcagccc
    12901 gtcgaccgga ccgtacgcga ccaggaacgc ctcgcgcgct gcaccaacca gggccccata
    12961 ggcgagcgac aggtcggccg aggggtcgcc ggcgcagacg tcgccccagt cgatcacgcc
    13021 ggtcggccgc tccccatcga ccagcacgtg ccgcacgtgc aggtcaccgt gcagcaccac
    13081 gacgcgcggc gacggtggca ggtcccgcgc atcgcgcagc agcgcgtcca ccgcggccgt
    13141 cggctcccac agcccggccc cgacgacgcg cgccagccgc tcgcgcgtca ggcgcacccg
    13201 gtgcgccatg tcggtgcgcc gcatcgggtc gatcggcagg gtcgggccga tgcgggagcg
    13261 cagcatcggc ccatgcaggg cgcggaggaa gcgacccagc tcccgtccga tcgtcgcccg
    13321 acgctcgtca ggcagaccgg aggaggccag ctcgacgccg gggaggtgct cggtcccgaa
    13381 ccacggccac gggtaacgct cgtccggatc accgacccag cggggcacgg ggacgggcag
    13441 cggcaggtgg ggcccgatcc gctcgagcgt ctcgatctcc cgacgaacgc cgggcacggc
    13501 gatctcacgc tgcgggaagc ggaagaccca gcgcccgtcg accacgtaca cgacgttgtc
    13561 ccagccctcc gccagccgct cgatgcgcgc gccacgcagc tccggcacac gcgcctcgat
    13621 caacgcgcgc gccaggtcag ggtcgacgac gcgctcggga tcccactcag gcacgccgga
    13681 caccgtagcg gagaggcgcc caggtagcgc tcgggtcttg gagtatatac ggatatactc
    13741 caaggctccc cactacgcca cccgcttggt cttcttgctg acgtagtcca cgagcacgta
    13801 ctcgcccagg tgctccggct cgacgtagaa cgcccggccg cggttgatgc ggctcatcag
    13861 gtcgacgaag tccgacaggt agcgcgaccg ctcgagcatg aaggtgttga tcacgatgcc
    13921 ctcgcgggtg catcggccca cctcgcggag cgtggactgg accgtgcgca gcgtgggcgg
    13981 gtacgcgaac tcgacctgcc cgccctcgat gtgcgccgtc ggctcaccgt cggtgatgac
    14041 gaccatctcc ttgttgccac cggtggatcg gcccagcagt tgccgcgcga tgatcagcgc
    14101 gtgctggagg ttcgtgccgt actcgaactc gtacgggctc agcgtcggga tcgactcggg
    14161 cttcagctgc cgcgcgtagt acgcgaagcc caccacgtac aggcggtcac gcggatactt
    14221 ggtgtggatc agcgtgtgga gcgccatcgc cacccgcttg gcggccgatg agcagccccg
    14281 cagcagcatc gagcggctca tgtcgagcag aaggaccgtc gacgacgtgg tcgtctcctc
    14341 cgtgcgcgcc acctcgaagt cggccggcgc gatgctcacc ggcgtccccg ggccgccccg
    14401 cgccatggcg ttgaacagcg tgcggttgat gtcgagcgcg aacgggtccc cgaactcgta
    14461 gggcttggtc tcctcggtcg gctcgccgcc gcggccggcg gcgggcagcg gatggccgcc
    14521 gaacccctcg cggcgcagcc ggctgaagat atcggtcagc gcccgcatgc cgagcttgcg
    14581 cgccgcgcga ggcgtgagct ccaggcggcc gccgtcgcgg tcgaggtacc cggcctcctc
    14641 cagcgccttc gtcagctcgc gcagctgctc gaggtcatcg atggcgtcgt cgccggccag
    14701 gtcgcggagc gcctcggcgt cgaccttctc gagcccgtcg gggtcgcgcg cggctccgag
    14761 cgcctcctcc atggcgtcgt actgctgctg gcggtcgatg gcggccagcg catcggccag
    14821 gcccatcggc tcgtcgcccg cgaacggata gggctcgccg aagggcaggt cgggtcgagc
    14881 ggcctggagg tttccggcca gccgggccat gtcgatcttc agccgatcgt cgcgcagcag
    14941 gtcgtccatc aggtcgcgca gctcctcgcg catctgcggg ctcatcgagt tgagcagcga
    15001 cgccatctgc gaggcctggc ggtggaggtg ctcgatcagg gcatcggtgt cggagatgcc
    15061 gggcgggaag tagctgccgt gcttggccat gaaggcggcg aagtcctcgg tggtatcggc
    15121 gccggtggcg tgcttctcca gcagctggtt gaggtcgcgg accatctctc gcacgccggc
    15181 gaggtcctcg ggcgtgatgc gctcgacccc ctccttcagc ccctggaagt aggtctgcag
    15241 catctgctgg cgcagctcgt cggtcagcgc gttgaaggcc tcgcgagcgc cgtcgtccat
    15301 gaactcgtag tcccgcagcc catggagccg gccggccagg ttggggggca gcgcgtccag
    15361 ccggttctcc cgctgccgcg cggtgcggcg cacgacctgc tccgccatcc gcgcctggtc
    15421 ctgctcaggc cccggcggcg cgtcgagggc gcgctgctcg gcggccttcg tgcgctcctc
    15481 gatcccccgt cgctcctgct tcaggatctc ctcgagccgc tggttgagat cctcgagaac
    15541 gccgtccagg ttgctgcgct cgagctcctc ctgccgctgg gcgcgcagcc gctcgaggat
    15601 ccgtcgcagg cccgggatgt caccgccgcg cgtcccgcgc aggccgcgct gcatcagccg
    15661 ctggagcgcc cgtcggacgt cgccctcggc caggatgtca tcggagaggg cgtcgagcac
    15721 gtcgtccgcg tcgaaggccg gcagctgctg gctgccgtcc catcgcgagt agcggaacgt
    15781 cgtcatggtc gcgacagcct aggccgatgc cggggtcgcg tccatctggc accccgcgcc
    15841 ggtcaccggt ccagcgacgc gatcggtacc ggttctggcg gagcgccctc cggctcggcg
    15901 aggcggagcc agtaccacgc cacgtcgtgc caggcgccga acttccagcc cacgcgctcg
    15961 tatcgggcaa cctgtcgcat gcccatggcc gcgtgcaggg cgacgctggc cgggttcggc
    16021 agcgcgatcc ccgcgtaggc gttcacgtag ccctggcggc gcagcaggtc gaagaggccg
    16081 tcgtagagcg cccgcccgat cccctgcccg tgccacgccg catccacgta gaccgacacg
    16141 ttggccgacc agcggtagcc ggggcggtcg cggtggggtc cggcgtaggc gtagccaacc
    16201 acgttcgcgt ccagctcggc gacgagccac gggaagcgct ccagggtcgc gctcgtgcgg
    16261 cgggccatct cgtccgcagt cggggcggct tcctcgaagc tcgcgatcgt cgagtcgacg
    16321 gccgggcggt agatggaggc gatggcctcg gcgtctcgat cgggctcggc aaggcgaagc
    16381 cgaaggtcct gggcgggcat ggccggcatt gtccgggatg cggcacgcgg cgtgctggct
    16441 cgggcgagca tgagcagcaa cgacatccac ggcatcgagt cgggctggtc ggtgaagacc
    16501 gccgatggcg agggcatcgg ctccgtcgag gagacgacgg accgatacat cctcgtcaag
    16561 tcgggcctca tcaacgccag ccggcactac ctccccgcgg tcgagctgga gcacgtgcgg
    16621 ccggagcaga aggagatcgg cgtctcgctg acgaaggcgg agttcgaggc cggcgactgg
    16681 tcggagccac cgacccaggg gccgcgcgcg gacgtgccgc tcaaccctga caccgcccgc
    16741 gacgaggaca acccgatccg ccatcagccg gtcgacccgg agcgacccat cagcctctga
    16801 cggcggtcgg ctgacctcaa gcgtccggcg accgaattcg gcaccatagg cgccgatgac
    16861 gcggcgtgcc ctcttcctcg tcctcccatt cgcgctggcc gtgccggcag cgtacggtct
    16921 cgccttcgcg tacgcgggat atcccctcga gctcggtccc ctgctgatcg gcgcgctggg
    16981 ttgggtcctg gccctggtgt tgcgcgcgcc cgtcgggctc gcggggctga agatcaccgg
    17041 ctcgcaggag agcgctcagc ggtgggtcgt cgcctcttcg gggccgctgg aggagtccgt
    17101 gcggctcgcg gccttgctgc tggtcgggcg tgatctcccc acggccctct ggctgggcct
    17161 cggctgggcg gccatcgagg tcggctacgc gatcgccaac ggcttcgcca tggcggccct
    17221 ggcgtcccgc accgacccgg aggcggagcg cgcccgcgcg atgctcccgc cgtcggcctt
    17281 cgcggcctcg gcgccgtggt ggggcgtggt cgagcggatc tgggcgtcgg ccctgcacat
    17341 cggcttcacg ctgatgctgg ccgccgcgcc gatcctgtgg gtcgtgaccg tgccgctgca
    17401 cacgctgacc aacgtgacct tcgtgcgcct cggccagcgc cgctcgatgc tcatcgtcag
    17461 cgggctgggc ctgctgttcg gggccatcat cctggccggc ggcctggcgc tccaccgcat
    17521 cggctgagcc gtcatccgcc cgttcgcctt gcgggcgagg cgatcgtcgg acgatactct
    17581 ggctccccgc ccacctggag gacgcccttg atctgcacgg cctgcggcgc cgagaacgat
    17641 gccggacgca agttctgcct ccagtgcggc cagcgcctgg ccgtcgcctg cccgagctgc
    17701 ggcacggcca accccgcggg cgcgcgtttc tgcggcgagt gcggcgggcc gctcggcgga
    17761 gccgatgcga tcgccggcgt gccggccgtg tcgtcagggg catccaccgg tccggcccaa
    17821 tcgcggacga cgacggccac tgagcgccgc ctggtgagcg tcctcttcgc cgacctggtc
    17881 gggttcacca ccatctcgga gtcccgcgac gccgaagacg tccgggcact gctcgaccgc
    17941 tacttcgcca cctcgcgcga gatcgtcgag cgctacggcg gcatcgtcga gaagttcatc
    18001 ggtgatgcgg tgatggccgt gtggggcacc cccgtcgccc aggaggacga cgcggaacgg
    18061 gcggtgcgcg ccgcgctgga cctggtcgat gccgcgcgcg ccctcggaca gcaggccggc
    18121 gtgccggacc tggcgctgcg cgcgggcgtc ctgaccggcg aggccgtcgt gacgatcggg
    18181 gcgacgggca tgggcatggt cgccggcgac gtggtcaaca ccgcgagccg gctccagtcc
    18241 gtctcggcgc cgggcagcgt cctggtgggc gaggcgacgc gacgcgcggc gagcgaggcg
    18301 atcgtcttcg agccggcggg cgagcagacg ctcaagggca aggagtcgcc cgtcccggcg
    18361 ttccgggcgc tgcgcgtcgt ggcgcaacgc ggcggcgtcg gtcgcagcga gctgctcgag
    18421 ccgccgttcg tcggccgcga cccggagctg cggctgatca aggacttcta ccacgcgacg
    18481 gcgcgcgagc aggggccgcg cctcgtcagc gtcatcgggc aggcgggcat cggcaagagc
    18541 aggctcgcct gggaattcct gaagtacatc gacggcgtca cggagaacgt ctggtggcac
    18601 gagggccgct cgcccgccta tggcgacggg atcagcttct gggcgctggg cgagatggtg
    18661 cggatgcgcg ccggtatcgg cgaaggagcc gatgcgggca cgacccgaga gcggatctcg
    18721 gcgtcgctga ccgagttcgt ggtcgatccg gacgagcgag cgtcgctgga ggggccgctg
    18781 ctgcacctgc tcgggatcag cgacgagacg gcgttcgagc gcagcgcgct cttcgtggcg
    18841 tggcggacct tcttcgagcg catcgcggac cagggtccgg tggtcatggt cttcgaggat
    18901 ctgcagtggg ccgatgacgg cctgctcgac ttcatcgagg acctggtgac ctggtcgcgc
    18961 ggacgctcga tctacgtcat cacgctggcg cgcccggagc tgctcgaccg gcggccgacc
    19021 tggggcgccg ggctgcgtgc gttcacctcg ctcagcctgc agccgctgtc cgacgaggac
    19081 gtccgcacgc tcctcgacgg gctggtgccg ggcctgccgt ccaacgtcac ggaggcgatc
    19141 gtggcgcgcg cggagggcgt gccgctctac gcggtcgaga ccgttcggat gctcctcaac
    19201 gacgggcgca tcgagcgcga cggcgaccgg ttccgcccgg tcggtgacct gtccgcgctc
    19261 gccgtcccgg agtcgctgca cgcgctcatc gccgcccgaa tcgacgcgct gccggcggcc
    19321 gagcgtgcgc tgctgcagga cgcatcggtc ctgggccagt cgttctcgcg agaggcgctc
    19381 gtggcggtga cccgcgtgga cgagggcgag gtggacggca tgctgcgcca cctggcccag
    19441 cgcgagctga tcgacgtcga cgatgatccg cgttcgccgg agcgaggcca gtaccgcttc
    19501 gtgcaggggc tgctgcggga ggtggcctac ggcaccctgg cgcgcgacga tcggcgcacg
    19561 cgccacctgg ccgccgcccg ctactacgag acgctcggcg acgacgagcg ggccggtgtg
    19621 ctggcccagc actacgtcga cgcgtaccag gcccacccgg acggccccga gggggcggcg
    19681 gtggccgccc aggcacgggt cgcgctgcgg gccgcgtccc agcgcgcggc cgcgctcggg
    19741 tcgcaccgac gggccctgca gtacatcgag agcgcgctgt ccgtgaccac cgatcagcga
    19801 gaggagctgg atctgcgggt ggccgccggt acgcgcgccg gcgacgccgg cctgatggag
    19861 ccggccgtca cgcacctcga gcgcgccatc gacatcgcgc ggctgctcgg cgagcactcc
    19921 atccgacgcc gcgccacgac gacgctggcc gacgtcctca cggagggacg gcagaaggag
    19981 tcgcagcagc tcctcgagca gctgctcgcg gagccggact tcgacgaatc ggatccggaa
    20041 tacctcaatg ccgcgaccca gctggcgaag gtccacatgc gccgcctgga ggacctgaag
    20101 gcgatcgagc tcgccgatcg catgctgccg ctggtcgaac ggggcggcga ccaggagctt
    20161 gccctcgacc tgctcatcac ccgtggggtg gccctcggca acctgaaccg caacgtcgag
    20221 gcgacggccc tgatccaggg cgccctcgag tcggcgctgc gcggcggtca cgtggatgtc
    20281 gccctgcggg gctacacgaa ccttggctac gtgctggcca gcgacgatgt ccaggccgcc
    20341 caccgcgtct cgcgcgaggg tgtcgagatg gcgatcaaga ccgggaagac gtggcccatc
    20401 cgctacctgg tcggcaacgc catcgacggg gcgctcgaga tcggcgagtg ggactggctg
    20461 ctccggaccg tggccgaaca ggatcagggc gagctcgagc tggcggagcg catctggtac
    20521 tcggcgtatc ggctcctggt cgagaccttc cggggcgagg aggtcgagca cgaggcgggc
    20581 gagatctacg aggcgagccg aacggtcgac gacctccagt acctgctgat cggctcgtac
    20641 ccgatgacgg catgggccct cgtcaacggg cactacgacc gcgtcatgca gatctcggac
    20701 gggctgctgg gccagggacc gatcaacgcc gccgaggtcg ccgcgtgggc cgcgcgcgcc
    20761 gcggcctggg cgggcgacgt cgaggcgctg cggcgctacg cggaggcgtc gcgcgcgaca
    20821 cgcagcggac gacgcagcga ggtcctgctc cacgccacca gcggccagct ggccgccctg
    20881 gagggtcgcg acgcggatgc gcgcgccgcc ttcgcggcgg cgctgaggct gtcacgcgac
    20941 ctcggtctgc gcttctggac ggggctgatc gcgctcgacg ttctgaccct gggcgcgatg
    21001 gaacccgacg aacgcgcccg cgcggccgac gaggcacgcg tcatcttcac ggacctgggc
    21061 gcaacgccgc tgctgcggcg cctggaccag gtcgggccgc ccaccagcca gcgcggagga
    21121 agtgcgcggg ggcaggaggc gccgaccgag gctgccgagg taccgggcga ggcgtgacgc
    21181 tcgcgcagcg cgccttcatc gccgccaccc ggctgggagc gcggccggag gactcggccg
    21241 tcgaccggct gcagcggggc ctgctcgtca gcgtcctggt gatcgtcatc ggaccctgcc
    21301 tcgcctgggg cgcgctgtac tggctcaacg gcgagcgggt cgcggcgctg gtgccgtgga
    21361 cctacatcgg gctctgcgtc gcgtcgcttg ccgtcctggc gacgaccggg gcgttccggc
    21421 tcgtccggtc cgtccagctc accctcatcc tcgtgctgcc gatgaccctg tgcgtcctgc
    21481 tcggcggcat cgtgccgtcc agcggcgtcg tcctgtggtc cttcctggct cccgtggcgg
    21541 cgatcgcgtt cgatcgcccg gaccgagcgt ggcgctggtt cgccggcttc atcggcgtgc
    21601 tgctgctcgc gatcgtgctg ccgccgttcg tccgtccgga gccggcggcg atgcccgacg
    21661 ccatggtgct ggcgttcgcc ggcctgaaca tcggcgccgt gggactgctg tgcttctccc
    21721 ttctggcggc gttcgccacg gagcgggagt ccgcgcagcg acgggtcgag gagctgctcc
    21781 tcaacgtcct cccgcgcggc atcgtcgagc gtctgcagac cgaacgcggc tcgatcgccg
    21841 accacttcga ccaggccagc gtcctgttcg ccgacgtggt cgacttcacg ccgctctcac
    21901 agcggcttcc gccgcgcgag gtcgtcgaca tgctcgaccg cctcttcacc gagttcgacg
    21961 cgctggccga tgcgctgggc gtcgagaaga tcaagacgat cggcgactgc tacatggcag
    22021 cgtcgggggt cccggtgtcg cgggccgatc atgcggtggc gatcgctcgc ctggcgctgt
    22081 ccatgcgtga gtgtgtcggg cgattcccgc gggcggacga cgggcggccg ctgaggattc
    22141 gcatcggcct caatgccggc ccggtggtgg ccggcgtcat cggtcgtcgc cggttcctct
    22201 acgacctgtg gggt
//
LOCUS       DASTYP010000109         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_481207, whole genome shotgun sequence.
ACCESSION   DASTYP010000109 DASTYP010000000
VERSION     DASTYP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481207"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFV59_05210"
     CDS             <1..650
                     /locus_tag="VFV59_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEU5325263.1"
                     /translation="TNDLVDRSRDQAAARLEKPLAAGDISPEGALWVASLGLGLQLAA
                     SLRIGELALVLGLVAVASGLAYNVWLSRTPWSVIPYLVSFGVLPLWVAAGVGVPLERV
                     AVAPLLVGPFAAAAHLANTLRDFEGDQATGSRNLAQLLGRTTSFRVAWVLAMAVGLGV
                     GVAFALGGRVELVGGALGLVGLIAVAQGYAGSERLWGGMLVAAVCWTAAWALGSG"
     gene            complement(917..1069)
                     /locus_tag="VFV59_05215"
     CDS             complement(917..1069)
                     /locus_tag="VFV59_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325264.1"
                     /translation="MKAVKVLLISIAALVAAMIVIPLTILAGLFVWLKLTEETDDEEM
                     ELDDAV"
     gene            complement(1087..1611)
                     /locus_tag="VFV59_05220"
     CDS             complement(1087..1611)
                     /locus_tag="VFV59_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325265.1"
                     /translation="MRIYDGSPRQDWEEVLRSIGAFVDQERLKDLLLLELDGGFLLQG
                     LAMGAGGADSDAFGSLSKRTYELTDDQVAELMDASEARRGSSESDRPKPDLANYYELA
                     LRIVGKYIDGQRAHDVFLFEQDGSFVVRLFGGAGTHGAGHELAEFTREEILAMIEAAP
                     EQRIQPAPNPSGAP"
     gene            complement(1690..2607)
                     /locus_tag="VFV59_05225"
     CDS             complement(1690..2607)
                     /locus_tag="VFV59_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEU5325266.1"
                     /translation="MNARPDWLSVSEEVATALAEGRPVVALESSLIAQGLPVPHNLET
                     ALAAEEAVRESGAVPATTAVEDGRVVVGADRALLERLADPQRQIAKAASRDLGPLLAR
                     RALASTTVSATMRVAHATGIAMFATGGIGGVHRGAATTFDVSSDIDELATTPVAVVSS
                     GAKSILDLPATLEMLETRRVPVVGYGVDELPAFYSTTSGLRLPHRVDGPQEAAAAIAA
                     HRSIPASGGFLLVQPPPADLAIDPAEVDAWIADALHDAETRGIRGGAVTPHLLSHLAQ
                     ASGGRTLRVNIGLIVGNARTAGRVASALT"
     gene            complement(2604..3515)
                     /locus_tag="VFV59_05230"
     CDS             complement(2604..3515)
                     /locus_tag="VFV59_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEU5325267.1"
                     /translation="MRLRAAVMGDAVVDVHVSGTSPRVGSDVPAAIRLEPGGQGANLA
                     VRLARRGVDVRLGCSLADDAAGRMLRERLTADGVEMMVAPAERTGAVVVLVDADGERS
                     MLSQRVPLTPALDAIFLGHLVADLDWLAVSGYVLLEPDAEVLARSLVTTPARRVVLGC
                     ALRPVEAARWHASAAACRPSLLVLNADEAATVAGTTDPADAPARLAKDLAPAVVVTQT
                     GGATAVIDGTQVAAWGQVAEEAVDTTGAGDAFAAALLATIGRDHLTARILEAALTAAV
                     QAGSAATRVIGAQGRIPGERDADRLHS"
     gene            complement(3522..>3875)
                     /locus_tag="VFV59_05235"
     CDS             complement(3522..>3875)
                     /locus_tag="VFV59_05235"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEU5325268.1"
                     /translation="RALLPAVRATLIQLAIERRRADEAHNALHRQLGERLGPGDPQRQ
                     RLEARTGEYRAHIRALLEHLESMGVVIRDLESGLVDFPTLRDGEAAWLCWRLDDPDLA
                     FWHTTREGYASRRPL"
BASE COUNT          518 a         1377 c         1438 g          542 t
ORIGIN      
        1 ccacgaacga tctggtcgac cgatcccgcg accaggcggc ggcgcgcctg gagaagccgc
       61 tggcggccgg cgacatctcc ccggagggag cgctgtgggt cgcgtcgctg ggactcgggc
      121 tccagttggc cgcctcgctc cggatcggcg agctggcgct ggtgctgggg ttggtcgccg
      181 tcgcatcggg gctggcatac aacgtctggc tgtcgcgaac gccgtggtcg gtcatcccct
      241 acctcgtgag cttcggcgtg ctgccgctgt gggtcgcggc gggcgtcggc gttccgctgg
      301 agcgcgtggc tgtggccccg ctgctggtgg ggccgttcgc cgccgccgcg cacctggcga
      361 acaccctgcg cgacttcgag ggcgatcagg cgaccggttc tcgcaacctg gcccagctgc
      421 tgggacggac gacatcgttc cgggtggcgt gggtgctggc gatggccgtc ggtctggggg
      481 tgggcgtcgc cttcgccctc ggtgggaggg tcgagctggt cggtggggcg ctgggcctcg
      541 tcgggctcat cgcggtcgcg caggggtacg cggggagcga gcgcctgtgg ggcggcatgc
      601 tggtcgcggc cgtgtgctgg accgcggcct gggcgctggg cagcgggtag cggctccacg
      661 aatccgggcg tgggtttgcg tcgggttgcg ccaggtgcaa gtaacgcccg acgtcggcgc
      721 catctcgacg ctgccttgcg gccggtgcaa accggcgcca tcagcaggcc tgcacgcggg
      781 tcatctccgc agttacttgc cgccgcggca aagcgccacg gagcacgcgc cacctgacgg
      841 cgccacatca ccccgagaac gaagaagcgg ccccggatcg ggaccgcttc aggcgcgaag
      901 agatgcgacg gacgtgtcag acggcgtcgt cgagctccat ctcctcgtca tcggtctcct
      961 cggtgagctt cagccacacg aagagccccg cgaggatggt gagcgggatc acgatcatcg
     1021 ccgccaccag ggccgcgatg ctgatgagta ggaccttgac cgccttcatg ggaaggatct
     1081 cccctgctac ggcgcgccgc tggggttggg cgcgggctgg attcgctgtt ctggtgctgc
     1141 ctcgatcata gccaagatct cctcccgggt gaactcggcg agctcgtgtc cagcaccgtg
     1201 cgtgccagcg ccaccgaaga gacggacgac gaaggacccg tcctgctcga agaggaagac
     1261 gtcgtgggcg cgctggccgt cgatgtactt gcccacgatc cgcagcgcca gctcgtagta
     1321 gttggccagg tcgggcttcg ggcgatccga ttccgatgag ccgcggcgcg cctcggacgc
     1381 gtccatcagc tcggccacct gatcgtcggt cagctcgtac gtccgcttcg agagtgagcc
     1441 gaaggcgtcc gaatcggctc cgccggcgcc catggccagc ccctggagca ggaagccgcc
     1501 gtccagctcg agcagcagca ggtccttcag ccgctcctgg tcgacgaagg ccccgatcga
     1561 gcgcagcacc tcctcccagt cctgccgcgg gctgccgtcg tagatgcgca tggtcgaacc
     1621 cgtccctccg ctccgcccac cggctctcgg ctcatcggtg agtccgagcg agcatacgct
     1681 ccgccggggt caggtcaagg ccgatgcgac ccgtccggcc gtccgcgcgt tcccgacgat
     1741 gaggccgatg ttcacgcgca gcgtcctccc gcccgacgcc tgcgccaggt gcgacagcag
     1801 gtgcggggtg acggcgccgc cccggatgcc cctcgtctcc gcgtcgtgca gcgcgtccgc
     1861 aatccaggca tccacctcgg ccgggtcgat cgccaggtcg gcgggcggcg gctgcacgag
     1921 caggaagcca ccggacgccg gaatggaccg atgcgccgcg atcgcggcgg cggcctcctg
     1981 gggcccgtcg acgcgatgcg gcagacgcag gcccgaggtc gtgctgtaga aggcgggcag
     2041 ctcgtcgacg ccgtacccga ccaccgggac gcggcgcgtc tcgagcatct cgagggtcgc
     2101 cggcaggtcg aggatcgact tggccccgga gctgacgacc gcgaccggcg tggtggcgag
     2161 ctcgtcgatg tcgctcgaga cgtcgaaggt cgtggccgcg ccgcggtgca ccccgccgat
     2221 cccgccggtc gcgaacatcg cgatgccggt ggcgtgggcc acccgcatgg tcgcgctgac
     2281 ggtcgtcgag gcgagggcgc gacgcgccag cagcggaccg aggtcgcgcg aggcggcctt
     2341 cgcgatctgg cgctgtgggt cggcgaggcg ctccaggagg gcccggtcgg cgccgacgac
     2401 gacgcggcca tcctcgaccg cggtggtggc cggaaccgcg cccgactcgc gcacggcctc
     2461 ctcggccgcc agcgccgtct cgaggttgtg gggcaccggc aggccctgcg cgatgagact
     2521 ggactcgagg gcgaccacgg gacggccctc cgccagtgcc gtcgcaacct cctcggagac
     2581 gctgagccag tcgggtcgcg cgttcacgaa tgcagcctat ccgcatcgcg ctccccggga
     2641 atgcggccct gcgctccgat gacccgcgtc gcggcactcc ccgcctggac ggcggccgtc
     2701 agggcggcct cgaggatccg ggcggtcaga tggtcgcgcc cgatggttgc gaggagcgcg
     2761 gccgcgaagg catcgccggc gccggtggtg tcgacggcct cctcggcgac ctgtccccat
     2821 gccgccactt gcgttccgtc gatgacggcg gtcgccccgc cggtctgggt caccacgacg
     2881 gcgggggcca ggtccttcgc gaggcgcgcc ggagcgtcgg cggggtcggt cgtgccggcg
     2941 actgtcgccg cctcgtcggc gttgaggacc agcagcgacg gccggcaggc agccgcggaa
     3001 gcgtgccagc gcgccgcctc gaccggccgc agggcgcagc cgagcacgac gcgacgagcc
     3061 ggcgtggtca cgagcgagcg agccaggacc tccgcatcgg gctccagcag gacgtagccg
     3121 gagacggcga gccagtcgag gtctgcgacg agatgaccga ggaagatcgc gtcgagagcc
     3181 ggagtcagcg gcacgcgctg gctgagcatc gagcgctcgc catcggcgtc gaccaggacc
     3241 accaccgcgc cggtgcgttc cgccggcgcc accatcatct cgacgccatc ggcagtcagc
     3301 cgctcgcgca gcatgcggcc ggccgcgtca tcggcgagcg aacagcccag gcggacgtcg
     3361 acgccacgcc gcgcgagtcg aaccgccagg ttcgccccct gcccgcccgg ttcgaggcga
     3421 atggccgcgg gcacgtcact tccgacccgc ggcgaggtgc cgctgacgtg gacgtccacg
     3481 acggcatcgc ccatgacggc ggcgcgcagg cgcatcggtc cctagagcgg acggcgcgag
     3541 gcgtagccct cgcgggtggt gtgccagaag gccaggtcgg ggtcgtcgag ccgccagcac
     3601 agccaggccg cctcgccgtc gcgcagcgtc gggaagtcca ccagtcccga ctcgaggtcg
     3661 cggatcacga cgcccatcga ctccaggtgc tcgagcagcg cccggatgtg ggctcggtac
     3721 tcgccggtcc gcgcctcgag ccgttggcgc tgcgggtcgc cgggtcccag ccgctcgccg
     3781 agctgtcggt ggagggcgtt gtgggcctca tcggcccgcc gtcgctcgat ggccagctgg
     3841 atcagcgtcg cccggacggc cggcagcagc gcccg
//
LOCUS       DASTYP010000110         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_7617, whole genome shotgun sequence.
ACCESSION   DASTYP010000110 DASTYP010000000
VERSION     DASTYP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7617"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFV59_05240"
     CDS             <1..351
                     /locus_tag="VFV59_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325269.1"
                     /translation="YVLTRDRDLAAALDERLVGAIAFFYNDAARLHQAVTLRPPQVVV
                     VDTGAVRQEYGDAGLGPVFDFLRHRAPAARLAVRPTAGVEHLVAAEAGPDVDLLPAER
                     EGCVEAVAASCGCS"
     gene            complement(362..862)
                     /gene="sodX"
                     /locus_tag="VFV59_05245"
     CDS             complement(362..862)
                     /gene="sodX"
                     /locus_tag="VFV59_05245"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HEU5325270.1"
                     /translation="MRPLRGLTGPLVACLALIGALAAALAATVGRAFDVVEVRGRSMT
                     PALSPGDRLLVLPLRRVPRVGEIVLASDPREPSRELVKRVAAVSGDRVTLRGDNPAAS
                     TDARTFGSVGVADVRWRAVARTWPPERIGPLRADLSPALESVDEGGEPACAFPEALIA
                     GDETPR"
     gene            complement(883..1314)
                     /gene="sodN"
                     /locus_tag="VFV59_05250"
     CDS             complement(883..1314)
                     /gene="sodN"
                     /locus_tag="VFV59_05250"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEU5325271.1"
                     /translation="MRSLRTLFRPSRVVDAHCDIPCGVYDPEQARIEAESCLRIIEKY
                     EGNEDPLFRTRCIHVKEERAELVKHHLDVLWHDYFKPEHLEKYPDLHETLWKATKQAS
                     KVKQSLDAGAARELLGMIDKIDEMWKATGGPEKTRVNGRPG"
     gene            complement(1537..2292)
                     /gene="fabG"
                     /locus_tag="VFV59_05255"
     CDS             complement(1537..2292)
                     /gene="fabG"
                     /locus_tag="VFV59_05255"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU5325272.1"
                     /translation="MFDLTGRTALVTGGSRGLGRAIVLALAGQGADVAINYRGNADAA
                     NEVADQVRALGRRAVLVQGDTSGGREACEAIVKAALDELGKIDILVNNAGITRDNLMM
                     RMDAEEWDAVIDTNLSGPFWMTRAIARPMMKARYGRIINMSSAAGRMGNAGQANYVAA
                     KAGLIGLTKTTARELASRNVTCNAIAPGLITTDLTADLPEAATEALKSLTPLGYVGDV
                     SDVAAAAVYLASDEARYVTGQVLGVDGGIVMGS"
     gene            complement(2292..>3065)
                     /locus_tag="VFV59_05260"
     CDS             complement(2292..>3065)
                     /locus_tag="VFV59_05260"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEU5325273.1"
                     /translation="LGATKAAAFDLAAACSGFVYALATATGFVRSGMYRNVLVIGVEV
                     LSRFINWQDRNTCVLFGDGAGAVLLQASDQPGGLTGFQLFADGSGFEGIIVPAGGSAC
                     PASPQTAALGQHFVQMAGRDVYRYATRQLTESALAAVRDAGLTMDDIDQFVFHQANLR
                     IIESVGKQLGMPMEKCFVNIEKYGNTSAASVPMALVEAIAAGRIHPGDRILMVAFGAG
                     FTAAAAVVEWTADPQRAFLAPGSQTRLGQPQRAVEEVAA"
BASE COUNT          415 a         1072 c         1114 g          464 t
ORIGIN      
        1 tacgtcctga cccgcgaccg cgacctcgcc gccgcgctcg acgagcgcct ggtcggagcc
       61 atcgccttct tctacaacga cgccgcccgg ctgcaccagg cggtgaccct gcgcccgccg
      121 caggtcgtgg tcgtcgacac cggcgccgtg cggcaggagt acggggacgc cggactgggt
      181 cccgtcttcg acttccttcg tcaccgcgct cccgcggcac ggctggcggt ccgccccacg
      241 gccggggtcg agcacctcgt cgcggccgag gcgggcccgg acgtggacct gctccccgcc
      301 gagcgcgagg ggtgcgtcga ggcggtcgcg gcgtcctgcg gctgcagcta ggggcgacgc
      361 gctagcgcgg cgtttcgtcg cccgcgatca gcgcctccgg gaaggcacac gccggctcgc
      421 cgccctcgtc cacggattcg agggccgggg aaaggtccgc ccgcagtgga ccgatgcgct
      481 cggggggcca ggtgcgtgcc accgcccgcc agcgaacgtc ggcgacgccg acgctgccga
      541 aggtccgggc gtccgtgctg gcggccgggt tgtcgccacg cagcgtcacg cggtcgccgc
      601 tcaccgcggc cacgcgcttg accagctcgc gggacggctc ccgcgggtcg gacgccagca
      661 cgatctcgcc gacgcgtgga acgcggcgaa gcggcagcac cagcagccgg tcaccgggcg
      721 acagcgccgg cgtcatggat cggccgcgca cctcgaccac gtcgaaggca cggccgacgg
      781 tcgcggcgag ggcggccgcg agggctccga tgagcgccag gcatgcgacg aggggcccgg
      841 tcaggcccct cagcggtcgc atcggtcgat tcgcccggaa ggtcagccgg ggcggccgtt
      901 gacccgggtc ttctccggcc cgccggtcgc cttccacatc tcgtcgatct tgtcgatcat
      961 cccgagaagc tccctggcgg caccggcgtc caggctctgc ttgaccttcg acgcctgctt
     1021 ggtggccttc cagagggtct cgtgcagatc cgggtacttc tccaggtgct ccggcttgaa
     1081 gtagtcgtgc cacaggacgt cgagatggtg cttcaccagc tcggcgcgct cctccttgac
     1141 gtggatgcat cgggtgcgga agagcgggtc ctcgttgccc tcgtacttct cgatgatccg
     1201 caggcaggac tcggcctcga tccgggcctg ttcggggtcg tagaccccgc acgggatgtc
     1261 gcagtgcgcg tcgacgacgc ggctgggtcg gaacagggtg cgcagagaac gcatcggtct
     1321 cctttcgccg gtgagcaatc gcttggctgt cgaagtgacg acattgtatg gcgctgcaag
     1381 cggcgcgccg ggttcggcga ttgcgcggga aggtgcagcg aagcccgggg tgaacccggg
     1441 cttcgtgcgg cggagggcgc caccatggat acggcgtgcc ggggagcgtg gatcagcgcg
     1501 gcacgaaggt gaccgtccga cggaagcgct cgggactcag ctccccatca cgatgccgcc
     1561 gtccaccccg agcacctgcc cggtcacgta gcgggcctcg tccgaggcga ggtacaccgc
     1621 ggcggcggcc acgtcgctga cgtcaccgac atagcccagt ggggtgagtg acttgagcgc
     1681 ctcggtcgcc gcctcgggca ggtccgccgt gaggtcggtg gtgatcagtc ccggggcgat
     1741 ggcgttgcag gtgacgttgc ggctggcgag ctcgcgcgcc gtcgtcttgg tcaggccgat
     1801 gaggcccgcc ttggcggcca cgtagttcgc ctggccggcg ttgcccatgc ggccggcggc
     1861 gctcgacatg ttgatgatgc ggccgtatcg ggccttcatc atcggcctgg cgatcgcgcg
     1921 cgtcatccag aacgggcccg acaggttggt atcgatgacc gcgtcccact cctcggcatc
     1981 catgcgcatc atcaggttgt cgcgcgtgat gccggcgttg ttcaccagga tgtcgatctt
     2041 gccgagctca tccagggcgg ccttgacgat ggcctcgcac gcctcccgac cgccgctcgt
     2101 atcgccctgg accaggacgg cgcggcgacc cagcgcacga acctggtcgg cgacctcgtt
     2161 cgccgcgtcg gcgttgccgc ggtagttgat cgccacgtcg gcgccctgtc cggccagcgc
     2221 caggacgatg gcgcgtccga gcccgcggct gccgccggtc acgagcgcgg tgcgtccggt
     2281 gaggtcgaac atcaggcggc gacctcctcg accgcgcgct gcggctgccc gagccgcgtc
     2341 tgggagcccg gagccaggaa ggcgcgctgt ggatcggccg tccactccac gaccgcggct
     2401 gcggcggtga agcccgcgcc gaaggcgacc atcaggatgc gatcaccggg atggatgcgg
     2461 ccggcggcga ttgcctcgac cagggccatg ggcaccgagg cggcgctcgt gttgccgtac
     2521 ttctcgatgt tcacgaagca cttctccatc ggcatgccca gctgcttgcc gacgctctcg
     2581 atgatccgca ggttcgcctg gtggaacacg aactggtcga tgtcgtccat ggtgaggccg
     2641 gcatcgcgca cggcggcgag ggcggactcg gtgagctgcc gcgtcgcgta gcggtagacg
     2701 tcgcgtccgg ccatctggac gaagtgctgc cccagggccg ccgtctgcgg ggaggcgggg
     2761 caggcgctgc cgccggccgg cacgatgatc ccctcgaagc ccgagccgtc ggcgaagagc
     2821 tggaacccgg tgaggcctcc cggctgatcc gatgcctgca gcagcacggc ccccgcgccg
     2881 tcgccgaaga gcacgcaggt gttgcggtcc tgccagttga tgaagcggct gagcacctcc
     2941 acgccgatga ccagcacgtt gcggtacatg ccgctgcgca cgaagccggt ggccgtggcg
     3001 agggcgtaga cgaagcccga gcacgcggcg gccaggtcga aggccgccgc cttcgtggcg
     3061 ccgag
//
LOCUS       DASTYP010000111         5063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1391669, whole genome shotgun sequence.
ACCESSION   DASTYP010000111 DASTYP010000000
VERSION     DASTYP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391669"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="VFV59_05265"
     CDS             <1..1747
                     /locus_tag="VFV59_05265"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5325274.1"
                     /translation="VAGAAAERAAAPPVPSLRSFAWHHAPMSSSFAEAQDAFFVDFFR
                     FYPYHATEAGNHQYDDVWPDLTDAGRRARLDWLAEVRARIQSAEAASREEGIDRAVLL
                     RQIDELRFDDEELDELSWSPIIYAYLFGGGLFGLLSREFAPLADRLASAAGRMERMPA
                     AIDAARANLTSGRGRPVARFHAEKAIETMPGVADLCRTAVSMAADVDQSLRERVSRAA
                     DAAIAAVDDFTAWLRDELLPTADGDFRLGAELFDRKFHHSLKATISPEELERRAAQAF
                     DDVRAEMLRIARELWPTWIGDEDLPDDPDVLTRRVLDAIAVDHPSAEELLDYCTAEHE
                     RILSFVRDRDLVGLPDEPLQIIWTPPFLRAFGGAMLIPPGPLDKGLDSFFAITPPRED
                     WSDEQVESMLRENNSRSLKVLTIHEATPGHYLQLAWSNRCDSLARAVFGSGVFAEGWA
                     VYVTQVMMDVGYADDDPALLLAHWKYFLRAATNTLMDIRIHRGGMTAEEAMTLMVDGG
                     FQERSEASEKWSRARLSSTQLCSYFLGAAEMTELEREARRRAGDAFEWRPFLESVLSH
                     GTPPMPVIRDILYGD"
     gene            complement(1754..2884)
                     /locus_tag="VFV59_05270"
     CDS             complement(1754..2884)
                     /locus_tag="VFV59_05270"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5325275.1"
                     /translation="MAAALLSLLAFVAPSLTLAPLQAVEPGLPAAPVDVAVERVVPRL
                     APHLLERAAPPAAADQQLGLALEAARATGSAYGVTFALVRDGVVTWTGAAGRARDGAT
                     PLTTDARFVIGSVTKTYVAATVLQLVAEGRLALSDPAVDHLSGVSISRDITVRQLLDH
                     TSGLADLFNDATRRGLEEHPGTAWTREEVFGSLHAPWYRPGEGWAYANTNYYLLGLIV
                     ESITGTSLADDLQQRLLGPMHLDATSVLTGATGEPLSPAWASIFWASGAMSASASDLA
                     RWGDALYDGRVLPTALRSQMLDWNDDDYGLGAQRIEVPGAVGYGHTGLLDTYTTLLLY
                     LPSADVTLALLVNRTNVDLGAMLTAHPAGDGPSLLDLALGRD"
     gene            3069..4460
                     /locus_tag="VFV59_05275"
     CDS             3069..4460
                     /locus_tag="VFV59_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325276.1"
                     /translation="MTITNLTDRLGRSIPTLLRTARRPDGAAPLPAADRGGGFRRGFT
                     ALGHPNYRRYWFGQLGSVVGSWMQSVALPWLVLQLGGSPIQLGLVMALMYGPSMFFAP
                     LGGVLADRVDKRRALIVVNAVSALQAAGLFVLAATGAVEIWHVYALALVAGFANAVEM
                     PVRQAFVAELVPREDLVNAIALSSTMFNLSRVVGPAVAGVTIAVFGVAVNFGINAVSY
                     LSVIAGMWLIDRRDLFRSEITQLPTSVRSSLAEGLRYARATPTVLWPLVLLGSVSALA
                     MNFQTLLPLYARNSLGLDSGGYGALFATMGVGSLAGSLLLAFATGQRPLLRLIIGGGA
                     AFLALAFLLGFVGTPLLAFPLVIGIGLASMLMVNTINVTVQNSVPDALRGRVMSLYVT
                     VFAGTAPIGGLVSGALAQALGAAAAFSIGAAAAGMVLAFVAWRLRSVQMPRHAVAGAS
                     ADAPVRRPTSVVA"
     gene            4533..>5063
                     /locus_tag="VFV59_05280"
     CDS             4533..>5063
                     /locus_tag="VFV59_05280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325277.1"
                     /translation="MAEPGSSRPDQPRSLPLRFSLGGLKRGFASLGIRNYRLYWIGQV
                     VSLVGTWMQQVSLPWLVLALGGTPIQLGLVAVLQFGPAMVLAPFGGVFADRVDKRKAL
                     MGTQAAAAVQAAILFALTITGVVEIWMVMVMALWLGLVNAVDMPVRQALAADLVPREL
                     LPNAIALNSMAFNSARV"
BASE COUNT          665 a         1833 c         1763 g          802 t
ORIGIN      
        1 cgtcgcggga gcggccgctg agcgcgcggc cgctccacct gttccgtcgc tccgcagctt
       61 cgcgtggcac catgcgccaa tgtcttccag cttcgccgag gcgcaggacg ccttcttcgt
      121 cgacttcttc cgcttctacc cgtaccacgc gacggaggcg ggcaaccacc agtacgacga
      181 cgtctggccg gatctgaccg atgccggccg ccgcgctcgg ctggactggc tggccgaggt
      241 gcgagcgcgc atccagtccg cggaggccgc cagtcgcgag gaagggatcg accgagcggt
      301 cctgctgcgc cagatcgacg agctgcgctt cgatgacgag gagctcgacg agctgtcgtg
      361 gagcccgatc atctacgcct acctcttcgg cggcggactc ttcggcctgc tgtcgcgcga
      421 gttcgcgccg ctggcggatc ggctcgccag cgccgccggg cgcatggagc gcatgccggc
      481 ggccatcgac gcggcgcgcg cgaacctcac ctccggccgc ggccgtcccg tcgcgcggtt
      541 ccacgccgag aaggcgatcg agacgatgcc gggcgtcgcg gacctgtgcc ggacggccgt
      601 ctccatggcc gcggacgtcg atcagtcgct gcgcgagcgc gtgtcacgtg cggccgacgc
      661 ggccatcgcc gccgttgacg acttcaccgc atggctgcgc gacgagctgc tgcccacggc
      721 cgacggcgac ttccgccttg gcgcggagct gttcgaccgc aagttccacc actcgctcaa
      781 ggccaccatc tcgccggagg agctggagcg ccgagccgcg caggcgttcg acgacgtgcg
      841 cgccgagatg ctgcgcatcg cgcgcgagct gtggccgacg tggatcggcg acgaagacct
      901 gcccgacgac ccggacgtcc tgacccgtcg tgtcctggac gccatcgcgg tcgaccaccc
      961 gtcggcggaa gagctgctcg actactgcac cgccgagcac gagcgcatcc tgagcttcgt
     1021 ccgcgatcgc gacctggtgg ggctccccga cgagcccctc cagatcatct ggacgccgcc
     1081 gttcctgcgc gcgttcggcg gcgccatgct catcccgccg gggccgctgg acaaagggct
     1141 cgacagcttc ttcgccatca ccccgccgcg cgaggactgg agcgacgagc aggtcgagtc
     1201 gatgctgcgc gagaacaaca gccgctcgct gaaggtcctc accatccacg aggcgacccc
     1261 cggccactac ctccagctcg cctggtcgaa ccgatgcgac tcgctggcgc gagccgtctt
     1321 cggctcaggc gtcttcgcgg agggctgggc cgtgtacgtg acgcaggtga tgatggacgt
     1381 cggctacgcc gatgacgatc ccgccctgct gctggcccac tggaagtact tcctgcgcgc
     1441 ggcgaccaac acgctgatgg acatccgcat ccaccgcggc ggcatgaccg cggaggaggc
     1501 catgaccctc atggtcgacg gcggcttcca ggagcgctcc gaggcgagcg agaagtggtc
     1561 gcgcgcgcgc ctcagctcga cgcagctgtg ctcctacttc ctgggcgccg cggagatgac
     1621 ggagctcgaa cgggaggccc gtcgccgcgc gggcgatgcc ttcgagtggc gtcccttcct
     1681 cgagtccgtg ctcagccatg gcacgccgcc gatgccggtc attcgcgaca tcctctacgg
     1741 cgactgagcg ggctcagtcg cgaccgagcg cgaggtccag cagcgacgga ccgtcgccgg
     1801 ccggatgggc ggtgagcatc gcgcccaggt cgacgttcgt gcggttcacc agcagggcga
     1861 gcgttacgtc ggccgacggg aggtagagca ggagcgtcgt gtacgtgtcg agcagcccgg
     1921 tgtggccgta gccaacggcg ccgggcacct cgatgcgctg cgcgcccagg ccgtaatcgt
     1981 cgtcgttcca gtcgagcatc tgcgacctca gcgcggtcgg cagcacgcgt ccgtcataca
     2041 gcgcatcacc ccagcgcgcc aggtcggacg cggacgccga catcgcgccc gacgcccaga
     2101 agatcgacgc ccacgccggc gacagcggct cgccggtcgc cccggtcagg acgctcgtcg
     2161 cgtccaggtg catcggtccc aacagtcgtt gctgcaggtc gtccgccaat gaggtgccgg
     2221 tgatggactc gacgatcagc ccgagcaggt agtagttcgt gttggcgtag gcccagccct
     2281 cgcccggccg gtaccagggg gcgtggagcg acccgaagac ctcctcccgc gtccacgcag
     2341 tgccggggtg ctcctcgagg ccgcgacgcg tcgcatcgtt gaacaggtca gccagcccgc
     2401 tggtgtggtc gagcagctgc cggacggtga tgtcgcgact gatcgagacg cccgagaggt
     2461 ggtcgaccgc gggatccgac agcgcgaggc gaccctccgc gacgagctgc aggacggtcg
     2521 ccgccacgta ggtcttggtg acgctgccga tgacgaagcg cgcgtccgtg gtgagcggcg
     2581 tcgccccatc gcgagcgcgc cccgcggcgc cggtccacgt cacgacgccg tcgcggacga
     2641 gcgcgaaggt cacgccgtac gcggagccgg tcgctcgggc cgcttcgagc gcgaggccca
     2701 actgctggtc cgcggccgcg ggtggcgctg cgcgctcgag gaggtgcggt gccagccgcg
     2761 ggacgacgcg ctcgacggcg acgtcgaccg gtgccgccgg cagcccgggc tcgacggcct
     2821 gcagcggcgc cagcgtcagg ctcggtgcga cgaaggcaag gagcgagagg agcgccgccg
     2881 ccacgggcgc acgaagaccc aggcggggcg ggtgccgtcg cgccccccgc gcacggatcg
     2941 ctcgggcctg ggtgcgatgt cgcgtcgtgc tcgcatcatg ccgcatccag cgcctcgcgc
     3001 gaagccctgt cgctcacggc gccccttgat tcgttcggcg aaagttgtta gcattgctaa
     3061 ccactcgcat gacgatcacg aacctcaccg atcgactcgg ccggtcgatc ccgaccctcc
     3121 tccgcaccgc gcgccgcccc gacggggcgg cgccgctgcc tgctgccgat cgcggcggcg
     3181 ggttccgtcg ggggttcacg gcgctcggcc accccaacta ccggcggtac tggttcggcc
     3241 agctcggcag cgtcgttggc tcgtggatgc agtcggtggc gctgccgtgg ctcgtgctcc
     3301 agctgggcgg ctcgcccatc cagctcggcc tggtcatggc gctcatgtac ggcccatcga
     3361 tgttcttcgc gccactcggc ggcgtgctgg ccgaccgcgt cgacaagcga cgcgcgctga
     3421 tcgtggtgaa cgccgtctcg gcgctccagg ccgcgggcct gttcgtgctg gccgcgacgg
     3481 gtgctgtgga gatctggcac gtctacgcgc tggccctggt cgcggggttc gcgaacgcgg
     3541 tcgagatgcc ggtgcgccag gcattcgtgg ccgagctggt gccgcgcgag gacctggtca
     3601 acgccatcgc cctcagctcc acgatgttca acctgtcgcg cgtcgttggc ccagccgtgg
     3661 cgggcgtcac catcgcggtc ttcggcgtgg cggtgaactt cggcatcaac gccgtgagct
     3721 acctgtccgt catcgcgggg atgtggctga tcgaccgtcg cgacctcttc cggtcggaga
     3781 tcacgcagct gccgacgtcg gtacgctcca gtctcgcgga ggggctgcgc tacgcccgcg
     3841 caacgcccac ggtgctgtgg ccattggtcc tgctcggaag cgtctcggcg ctggccatga
     3901 acttccagac gctgctgccg ctctacgccc gcaactcgct gggcctcgac tccggcgggt
     3961 acggcgcgct gttcgcgacc atgggcgtcg gctccctggc cggctcgctg ctgctggcct
     4021 tcgcgaccgg gcagcgcccg ctgctgcggc tgatcatcgg cgggggagcg gccttcctgg
     4081 cgctcgcctt cctcctcggc ttcgtcggca cgccgctgct ggccttcccg ctggtgatcg
     4141 ggatcgggct ggcctcgatg ttgatggtca acaccatcaa cgtcacggtg cagaacagcg
     4201 tccccgacgc gctgcgcggc cgggtcatgt cgctctacgt cacggtcttc gcgggcacgg
     4261 cgccgatcgg cggactcgtc tcgggcgcgc tggcgcaggc gctgggtgcc gccgctgcgt
     4321 tcagcatcgg cgccgctgcc gcaggcatgg tcctggcctt cgtggcatgg cgcctgcgct
     4381 cggtgcagat gccgcggcat gcggtcgccg gcgcctcggc cgatgcgccc gtgcggagac
     4441 ccaccagcgt cgtggcctga tgccgatccg cgtctggacc ggccccgccg cgatcgttag
     4501 catcatcaag ttcgtcggag ccctgaccgc ccgtggctga acccggttcc tcccgccccg
     4561 atcaaccgcg atccctcccg ctgcgcttct cgctcggtgg gctgaagcgc ggcttcgcct
     4621 cgctcgggat ccgcaactac cgcctctact ggatcggcca ggtcgtgagc ctggtcggca
     4681 cgtggatgca gcaggtcagc cttccgtggc tcgtgctggc gctcggcgga acgccgatcc
     4741 agctcggcct cgtggcggtc ctgcagttcg gccccgccat ggtgctcgca ccgttcggcg
     4801 gtgtcttcgc ggatcgcgtc gacaagcgca aggcgctcat ggggacgcag gccgccgccg
     4861 ccgtgcaggc cgccatcctc ttcgcgctca ccatcaccgg cgtcgtcgag atctggatgg
     4921 tcatggtcat ggcgctgtgg ctgggcctcg tgaacgcggt tgacatgccg gtccgccagg
     4981 cgctcgcggc cgacctcgtt ccccgcgagc tgctcccgaa cgcgatcgcg ctcaactcga
     5041 tggcgttcaa ctcggcgcgc gtc
//
LOCUS       DASTYP010000112         5366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_700023, whole genome shotgun sequence.
ACCESSION   DASTYP010000112 DASTYP010000000
VERSION     DASTYP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700023"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VFV59_05285"
     CDS             <1..133
                     /locus_tag="VFV59_05285"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU5325278.1"
                     /translation="FRSYVGEYCTSLEMAGAHLSLLKVDDEIKELLLAPAEVAYRVF"
     gene            348..1007
                     /locus_tag="VFV59_05290"
     CDS             348..1007
                     /locus_tag="VFV59_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325279.1"
                     /translation="MSHRRYASGWAAIAVAMITIGSLLIFAGGVTATQPEGLHCPDGW
                     MTKDDSGSDDNDLVPVAGLYICVKAGSPNSNETELGNTGIILTDGVSTLREYLFAAGI
                     VDGSGEFGRDVSYWVQYQAPTPTASPTPTPTPTPTPTASPTPSPTPTASPTPTPTPSP
                     GGGVLPGNPTPTPKPTARGAVLPSTSMPLPGIDLTLIGTLFLLAGTGFGAASLIERRR
                     R"
     gene            1164..1829
                     /locus_tag="VFV59_05295"
     CDS             1164..1829
                     /locus_tag="VFV59_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325280.1"
                     /translation="MFGFLRRWRTLAMVAIFAAVAAAIPMLSSHDHAAIAATSARTNR
                     AAFHDAMRRLWEDHIVWTRMFIVSAVADLPDLSQATDRLLANQDGIGSAIAPFYGEAA
                     GEQLTGLLRDHILGAAALLAAAKSGDEAAVEAASTAWYANGDDIAAFLNAANPRAWPL
                     DEMRAMMRGHLDLTLQEAVARLEGRYADDVAAYDRIHDQILHMADMLSDGIIAQLPSS
                     FAP"
     gene            complement(1880..2380)
                     /locus_tag="VFV59_05300"
     CDS             complement(1880..2380)
                     /locus_tag="VFV59_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325281.1"
                     /translation="MADRLDVRRRLRAADWPLWGGVALLVASMVLFAWQPTDVYATVN
                     VCSWCDLGWTSGVGVTGGWGIGSLGALIGWSMVAASLVFATWRPDREAPSWLRLVPVG
                     VMVAGVLVLVINLGEVLATAFGLLTLHLTLGVPAWIAGIALLTTWFRRNDGDLSALGR
                     LRNLRR"
     gene            2636..>5366
                     /locus_tag="VFV59_05305"
     CDS             2636..>5366
                     /locus_tag="VFV59_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5325282.1"
                     /translation="MALAESTGRFELAPTRENFSHIPEVLPLPNLIQTQTDSFHWFVE
                     TGLRELLDEITPITDFTGNNLELHFREYYFEEPKFSEEECRTRDLTYARPLKVEVDLV
                     IKETDEVKHQTVFMGDFPWMTEQGTFIINGAERVVVSQLVRSPGVYYTATEDPSTGRM
                     LYAAKVIPNRGAWLEFETSNKDLLSVKVDRKRKIPVTTLLRAVGLDTGEIERIFGPVD
                     DNPEHPYVQLTLEKDPAETQDQALIEVYKKLRPGDPPTVENARALVTSLFFNFRRYDL
                     ARVGRYKLNKALGETAKKLGTELPSEDAANPRESRVISRDDIVAIIGKLIELNNGRGD
                     PDDIDHLGNRRIRANGELIQNQFRIGLLRMERVVKERMTITDADQATPNTLVNIRPVV
                     AAMKEFFGGSQLSQFMDQTNPLAELTNKRRLSALGPGGLSRERAGFDVRDVHPSHYGR
                     MCPIETPEGPNIGLIGSLATYGQINRHGFIETPYRRVRRFVSHRDRKDDVLGQVLAED
                     LVLTDGGKPVAKAGTKIDEKIWTQLKEAKVAAARIRPVVTDEIEYLDAAEEERFYVAQ
                     ANAALTDDGHFAEERVLVRWKDKFLEEPPDVVDYMDVSPKQIVSVATAMIPFLEHDDA
                     NRALMGSNMMRQSVPLLQPEAPVVGTGVEYRAALDSQGVVVARAAGTVVSVTADWIVV
                     ERDEGDERDTYRLKKFLRSNQGTCINQRPVVDVGTRVAAGDVLADGSSTDGGEMALGQ
                     NILVAFLPWEGGNYEDAIVISERLVKDDLFTSIHIEKHEIEARDTKLGPEEITRDIPN
                     VGEESLRNLDENGIIRVGAEVKAGDILVGKITPKGETELTAEERLLRAIFGEKAREVK
                     DTSLRVPHGVRGKVIDVTVFSRENGDELPAGVNQMVRVSIAQKRKISIGDKMAG"
BASE COUNT          948 a         1915 c         1776 g          727 t
ORIGIN      
        1 cttccgcagc tacgtcggcg agtactgcac ctcgctcgag atggcgggcg cgcacctgtc
       61 gctgctgaag gtcgacgatg agatcaagga gctgctgctg gctccggcgg aggtcgccta
      121 ccgcgtcttc tgatccgatc cgtcgcgact ccacggcgcc ggcccgactc gggtcggcgc
      181 cgcttcgtgc ggcatccctg cgcccgcggt gccggggatc aacgcgcgcc aaccgggcca
      241 gtccagatgg cccttgtcgg tcatgggccg aacgtcctac gacggtccca gacccgcgct
      301 gagcgtcggc ggaggtccga cgcacacaca cgaaaggggg tgcggggatg tcccacaggc
      361 gctacgcgag cggatgggca gcgattgccg tcgcgatgat caccatcggc tcgctgttga
      421 tctttgcggg gggcgtcacg gcgacccagc cggagggcct ccactgtcct gacgggtgga
      481 tgacgaagga cgattccggt tcggacgaca acgatctcgt gcccgtcgcc ggcctgtaca
      541 tctgcgtgaa ggcaggctcg ccgaactcca atgagacgga gctcggtaac accggaatca
      601 tcctcaccga cggtgtgtcg acgctcaggg agtacctgtt cgccgcaggc atcgtcgacg
      661 ggagcggtga gttcggtcgc gacgtcagct actgggtgca gtaccaagcg cctacgccga
      721 cggcctcgcc aacacccaca ccgacgccga cgccgacgcc gaccgcctcg ccgacaccaa
      781 gcccgacgcc gacggcctcg ccgacaccaa ccccaacgcc aagtccgggc ggcggcgtgc
      841 taccgggcaa tcccaccccg acgcccaagc cgaccgcacg tggcgcggtc ctgcccagca
      901 cgtcgatgcc gctgccaggc atcgacctca cgctcatcgg caccctcttc ctgctcgccg
      961 ggaccgggtt cggggcggcg agcctgatcg agcgccgtcg ccgctgaccg atagcgcacc
     1021 gcatccgcga agggccggcc agaaggccgg cccttcgggc gtccgcgagg acggcgaccg
     1081 ctccacggtg taacgcggcc tgtataacca acatcagttg ccgatacaga tgcggcgccg
     1141 cacacctgga ggaggtctca cgaatgttcg gtttcctgcg ccgctggcgc accctggcga
     1201 tggtggcgat cttcgccgcg gtcgccgccg cgatcccgat gctctcgtcc cacgaccacg
     1261 ccgcgatcgc ggccacgagc gcccggacga accgcgccgc cttccacgac gccatgcggc
     1321 ggctctggga ggatcacatc gtctggactc ggatgttcat cgtcagcgcc gtcgccgacc
     1381 tgcccgacct gtcccaggcg accgaccggc tcctggcgaa ccaggacggg atcggctcgg
     1441 ccatcgcacc gttctacggc gaggcggccg gagagcagct gacagggttg ctgcgcgacc
     1501 acatcctggg agccgccgcg ctgctcgcgg cggcgaagtc cggcgacgag gcggccgtcg
     1561 aggcggcatc gaccgcgtgg tacgccaacg gcgacgacat cgcggccttc ctcaacgcgg
     1621 cgaatccacg cgcctggccg ctggacgaga tgcgggcgat gatgcgcggc cacctcgacc
     1681 tgaccctgca ggaggcggtc gcgcgcctcg agggccggta cgccgacgac gtggccgcct
     1741 acgaccggat ccacgaccag atcctgcaca tggccgacat gctgagcgac ggcatcatcg
     1801 cccagttgcc ctcgtcgttc gctccctgat cctcgacggg cgggtccatg cgtggaccgg
     1861 cccggccagg ccgcccgtgt cagcgacgca ggttgcgcag gcggcccaac gccgagaggt
     1921 cgccgtcgtt gcggcggaac cacgtcgtca gcagcgcgat cccggcgatc cacgccggca
     1981 cccccagggt gaggtgcagc gtgagcagcc cgaaggcggt tgcgagcacc tccccgaggt
     2041 tgatgacgag caccagcacg ccggcgacca tgacgcccac cggcaccagc cgcagccacg
     2101 atggcgcctc gcggtccgga cgccaggtgg cgaagaccag gctggcggcc accatcgacc
     2161 agccgatcag cgcgcccagc gagccgatgc cccatccgcc ggtcacgccg acgccgctgg
     2221 tccaacccag gtcgcaccac gagcagacgt tgaccgtcgc gtacacgtcg gtgggctgcc
     2281 acgcgaacag caccatcgac gcaaccagga gcgcgacgcc gccccacagt ggccagtccg
     2341 cggcgcgcag gcggcgccgg acgtcgaggc gatcggccac gggggcccgc gcggcggaac
     2401 gctgggcacc ggcagcttgc ggcgaggtcg agggtgacga ggcaggatcg gtcacgacgt
     2461 tcccccaggg cagtgtgcag aatcgcgcga gacggtagca tgccgctcac gaccgccggg
     2521 cccgttcggg acgacggttc caccgtgatg cgcttcagtt gacgaagtgc cacccggtga
     2581 tatactcctc gttcgcgtgt ccgcgcccca ccatgccctc tccgaggagt cctgaatggc
     2641 tttggccgag tcgaccggcc gcttcgagct ggccccgacc cgtgagaatt tctcgcacat
     2701 ccccgaggtt ctcccccttc cgaacctgat ccagacccag accgattcgt tccactggtt
     2761 cgtcgaaacc gggctccgcg agctcctcga cgagatcacc ccgatcaccg acttcaccgg
     2821 caataatctc gagctgcact tccgcgagta ctacttcgag gagccgaagt tcagcgaaga
     2881 ggagtgccgc acccgcgatc tcacctacgc gcgcccgctg aaggtggagg tcgacctcgt
     2941 catcaaggag accgacgagg tcaagcacca gacggtcttc atgggcgact tcccgtggat
     3001 gaccgagcag ggcaccttca tcatcaacgg cgccgagcgc gtcgtggtct cgcagctggt
     3061 gcgctcgccc ggcgtctact acaccgccac cgaggatccg agcaccggcc gcatgctgta
     3121 cgcggccaag gtgatcccga accgcggggc ctggctcgag ttcgagacct ccaacaagga
     3181 cctcctcagc gtcaaggtcg accggaagcg caagatcccg gtcaccacgc tgctgcgcgc
     3241 cgtcggcctg gacaccggcg agatcgagcg catcttcggc cccgtcgacg acaacccgga
     3301 gcacccgtac gtccagctga ccctcgagaa ggacccggcg gagacccagg accaggccct
     3361 gatcgaggtt tacaagaagc tgcgccctgg cgatccgccg acggtggaga acgcgcgtgc
     3421 cctcgtcacc agcctcttct tcaacttccg ccgatacgac ctggctcgcg tcggccgcta
     3481 caagctgaac aaggcgctgg gcgagaccgc gaagaagctc ggcaccgagc tgcccagcga
     3541 ggacgcggcc aatccgcgcg agagccgggt catcagccgc gacgacatcg tggcgatcat
     3601 cggcaagctg atcgagctga acaacggccg tggcgacccc gatgacatcg accacctggg
     3661 caaccgccgc atccgcgcca acggcgagct gatccagaac cagttccgca tcggcctcct
     3721 gcgcatggag cgcgtggtca aggagcggat gacgatcacc gatgcggacc aggcgacgcc
     3781 gaacacgctc gtcaacatcc gccccgtcgt ggcggccatg aaggagttct tcggcggcag
     3841 ccagctgagc cagttcatgg atcagaccaa ccccctggcc gagctgacca acaagcgccg
     3901 cctcagcgcg ctcggccccg gcggcctgtc gcgcgagcgc gccggcttcg acgtccgcga
     3961 cgtgcatccg tcgcactacg ggcggatgtg cccgatcgag acgcccgagg gcccgaacat
     4021 cggcctcatc ggctcgctgg cgacctacgg gcagatcaac cggcacgggt tcatcgagac
     4081 gccgtaccgg cgcgtgcgcc ggttcgtcag ccaccgcgat cgcaaggacg acgtcctggg
     4141 ccaggtgctg gccgaggacc tggtcctgac cgatggcggc aagccggtcg ccaaggccgg
     4201 gacgaagatc gacgagaaga tctggaccca gctcaaggag gcgaaggtcg ccgccgcccg
     4261 gatccgaccg gtggtcaccg atgagatcga atacctcgac gccgccgagg aggagcgctt
     4321 ctacgtcgcg caggccaacg ccgcgctgac cgacgacggg cacttcgccg aggagcgcgt
     4381 gctggtgcgc tggaaggaca agttcctgga ggagccgccg gacgtcgtcg actacatgga
     4441 cgtcagcccg aagcagatcg tgagcgtcgc caccgcgatg atcccgttcc tcgagcacga
     4501 cgacgccaac cgcgccctga tgggctcgaa catgatgcgg cagtcggtgc cgctgctcca
     4561 gcccgaggcc cccgtggtcg ggaccggcgt ggagtaccgc gccgcgctcg attcgcaggg
     4621 cgtcgtcgtc gccagggccg cgggcaccgt ggtcagcgtg accgccgact ggatcgtggt
     4681 cgagcgcgac gagggcgacg agcgcgacac gtatcggctc aagaagttcc tgcgctccaa
     4741 ccagggcacc tgcatcaacc agcgtcccgt cgtggacgtc gggacgcggg tcgcggccgg
     4801 cgacgtgctg gccgatggct ccagcaccga cggcggcgag atggcgctgg gccagaacat
     4861 cctggtcgcc ttcctcccgt gggagggtgg caactacgag gacgccatcg tcatcagcga
     4921 gcgcctcgtc aaggacgacc tgttcacctc gatccacatc gagaagcacg agatcgaggc
     4981 ccgcgacacc aagctgggcc ccgaggagat cacgcgcgac atcccgaacg tcggcgagga
     5041 gtccctgcgg aacctcgacg agaacggcat catccgcgtt ggtgccgagg tcaaggccgg
     5101 cgacatcctg gtcggcaaga tcacacccaa gggcgagacc gagctgaccg ccgaggagcg
     5161 cctgctgcgg gccatcttcg gcgagaaggc gcgtgaggtg aaggacacca gcctgcgcgt
     5221 gccgcacggc gtgcgcggca aggtcatcga cgtgaccgtc ttcagccgcg agaacggcga
     5281 cgagctgccc gccggggtca accagatggt gcgcgtgtcg attgcccaga agcgcaagat
     5341 cagcatcggc gacaagatgg cggggc
//
LOCUS       DASTYP010000113         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1318356, whole genome shotgun sequence.
ACCESSION   DASTYP010000113 DASTYP010000000
VERSION     DASTYP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318356"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1373)
                     /gene="moaCB"
                     /locus_tag="VFV59_05310"
     CDS             complement(393..1373)
                     /gene="moaCB"
                     /locus_tag="VFV59_05310"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HEU5325283.1"
                     /translation="MRPSHLDDEGAPRMVDVGDKPVTRRRAVAGGVVRMRPDVLVTLL
                     DAGGPKGDALVTARLAGIAGAKRTADLVPLCHPLPLDRVDVELTPDSMAGTVSIRATA
                     QATARTGVEMEALTAASVAALTLYDMAKALQRDIVIERIELLEKSGGRSGDYRRDAVA
                     APPPVPTAEHEAAVVTCSNRSAAGEREDTSGPALVDRLRQAGFDVAPDAHVVADDEER
                     IAALLATLADAGHRLILTTGGTGLTPTDVTPAATRRVVDRDVPGLAELMRSAGLASTP
                     MAALSRAVVGTRGSTLIANLPGSPKGATESLDALLPVLRHALDQLAGGDH"
     gene            complement(1370..2575)
                     /gene="glp"
                     /locus_tag="VFV59_05315"
     CDS             complement(1370..2575)
                     /gene="glp"
                     /locus_tag="VFV59_05315"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU5325284.1"
                     /translation="MLTLEEAQRRLLARAHLTDGERVPLADALGRVLVDPVVHAAADV
                     PPFANSAMDGFAVRAADTPGELRVVGEAAAGAPSVPQVDPGTAVAIATGGPMPEGADT
                     VVPIEEVEERGDVVRIPNPAGPGRHVRTAGHDTRRGEEVCLRGELTPAKIAVLASLGL
                     GDVEVHRRPRVAILSTGDELVEPGDRLAPHQIHDANGVALVAAVMDAGGEAVLRSRVR
                     DEAGPTESAMAEAARGADLVVTSGGVSVGRRDHVRAVLERQGALDFWRIAVQPGKPLA
                     VGELGGATVIGLPGNPVSALVTFELFVRPFLRAMLSLSGDGRVRVRATPTERMTKDPA
                     RRAFFRVRVSQEDTAIHAAPAGGQQSSQLRPMADANGLLVVPEGAEAAEPSHGYETIL
                     LGPVHLVGR"
     gene            complement(2587..>3716)
                     /locus_tag="VFV59_05320"
     CDS             complement(2587..>3716)
                     /locus_tag="VFV59_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5325285.1"
                     /translation="MGPGWRIGRIGGIDIAIHPSWLVIFFLVTFSLAESVFPREFRGW
                     STALYWGIGAITALLFFGSVLVHELSHALLARRFGLKVEGITLFIFGGATTIDSESRT
                     PREEALIALAGPVSSFLVGGVLLGIGAVVDQEQIRALTEWLGVINLLLGAFNLLPGFP
                     MDGGRVLRALIWRIRGDRLVATRNAAMVGRLFAYLLIGFGVYLALGPGNIFGGLWLAL
                     IGWFLSNAAEATAAQAGIERSLRGVRVRDAMEAEPPSVSPNETVGDLVQERLLRGEDR
                     SFLVRHDDGGLAGLVTLTDVRRLAREQWPQARVTDIMTRYADLATIGPDAPLADALRV
                     IQEREVGQLPVVEGADRRNPVGVVTRRGILRLIEARMKLGF"
BASE COUNT          534 a         1345 c         1376 g          461 t
ORIGIN      
        1 gccgaaccgg tcgatcggct gtccccggcg caggatcgcg aggtgccagc gcgctcgacg
       61 ccagaacagg aacgccgcgg cggcggccgc gacgaggacg agcgggtaga acgggatggt
      121 ggcgtgcatg ggtcggcgtc atggtagccg cgctccggcg tgcggtggcc gatggatcag
      181 tcatgtgcgc gctggcgtgc gggtgaccga tggatcagtc agatggcgcg gaaggcccga
      241 ttccggtgcg gcctggccga cgcatcggcc actgggacgc tgaagcggca ccgcgcggag
      301 cggtctacgc tccgccgcgg ccgacccatc ggtcatgcgg cgcgccaagc ggccgtccgg
      361 tgcgaattga ccgatgcagc agccgcgccg gcctagtgat cgccgccggc gagctggtcg
      421 agggcgtgac gcaggaccgg cagcagcgcg tcgagcgact cggttgcccc cttcggcgag
      481 cccggcaggt tggcgatgag cgtggagccg cgcgtgccga ccacggcgcg cgacagtgcc
      541 gccatgggcg tcgacgccag gccggccgag cgcatcagct ccgccagacc gggcacgtcg
      601 cggtcgacga cgcgccgcgt ggcggccggc gtcacgtcgg tgggcgtcag gccggtgccg
      661 ccggtggtca ggatgagtcg gtgaccggca tcggcgaggg tcgcgagcag ggccgcaatc
      721 cgctcctcgt catcggcaac gacgtgcgcg tcgggcgcca cgtcgaagcc ggcctgccga
      781 agccgatcca cgagtgccgg accggacgtg tcttcccgct ccccggccgc ggagcgattg
      841 gagcaggtca ccaccgcggc ctcgtgctcg gcggtcggca cgggtggcgg ggccgccaca
      901 gcgtcccggc ggtagtcgcc ggaccgtccg ccggacttct ccagcagctc gatccgttcg
      961 atgacgatgt cgcgctgaag agccttggcc atgtcgtaga gcgtcagtgc ggcgaccgag
     1021 gccgcggtca gcgcctccat ctcgacgccg gtccgcgccg tcgcctgggc cgtggcgcgg
     1081 atgctgaccg tgccggccat ggagtcgggt gtcagctcca cgtcgactcg atcgaggggg
     1141 agcgggtggc acagcgggac gagatcggcc gtccgcttcg ccccggcgat ccccgccagg
     1201 cgcgcggtga ccagggcgtc gcccttcggg ccgcccgcgt cgagcagcgt gaccagcacg
     1261 tcagggcgca tccgaaccac gccgccggcc accgcgcggc gcctggtgac cggcttgtcg
     1321 ccgacgtcca ccatgcgcgg ggcgccttcg tcgtcgaggt ggctgggtct catcggccga
     1381 ccaggtgaac tggaccgagg aggatcgtct cgtagccgtg cgacggctcg gccgcctcgg
     1441 cgccctccgg cacgaccagg agcccgttcg cgtcagccat ggggcgcaac tgcgacgact
     1501 gctggccacc ggcgggcgcc gcgtggatgg ccgtgtcctc ctgcgagacc cgaacgcgga
     1561 agaacgcgcg gcgcgccgga tccttcgtca tgcgctccgt cggcgttgcg cggacgcgga
     1621 cgcgcccgtc gccggacagg ctcagcatgg cgcggaggaa cggccgcacg aagagctcga
     1681 aggtgacgag ggcgctcacc gggttgccgg gcaggccgat gaccgtcgcg ccgccgagct
     1741 cgccgaccgc caggggcttc cccggctgga ctgcgatccg ccagaagtcg agggcgccct
     1801 gccgctcgag cacggcccgc acgtggtcac ggcgccccac gctgacgcca ccggacgtca
     1861 cgacgaggtc cgcgccgcgc gccgcctcgg ccatggccga ctcggtcggc cccgcctcgt
     1921 ccctcacccg ggagcgcagc acggcctcgc cgcccgcgtc catgacggcg gccacgaggg
     1981 ccacaccgtt cgcgtcgtgg atctggtgcg gggcgaggcg atcgccgggc tccacgagct
     2041 cgtcgccggt ggacaggatg gcgacccgcg gccggcggtg gacttcgacg tcgccgagac
     2101 cgagcgacgc gagaacggcg atcttggccg gcgtcagctc cccgcgcagg cagacttcct
     2161 cgccgcgtcg cgtgtcgtgc ccggccgtgc gcacgtggcg gccgggtccc gcgggattcg
     2221 ggatgcggac cacatcaccc cgctcctcga cttcctcgat cggcacgacc gtgtcggcgc
     2281 cctccggcat cggcccaccg gtggcgatcg cgaccgccgt gcccgggtcg acctgcggca
     2341 cggacggcgc gccagcggcc gcctccccca ccacgcgaag ctcgcccggc gtatcggcgg
     2401 cccgcaccgc gaacccgtcc atggccgagt tggcgaaggg cggcacatcg gccgcggcat
     2461 gaacgacggg atcgacgaga acccggccga gggcatcggc gagcggcacc cgctcgccat
     2521 cggtcaggtg cgctcgcgcc aggaggcgtc gctgcgcctc ctcgagcgtc agcatcggtc
     2581 cggccatcag aagcccagct tcatgcgcgc ctcgatcagg cggaggatcc cccgtcgcgt
     2641 gaccacgccc accgggttgc ggcggtcggc accctcgacg acgggcagct gaccgacctc
     2701 gcgctcctgg atgactcgca gcgcgtcggc cagcggcgca tcggggccga tggtggcgag
     2761 gtcggcgtac cgggtcatga tgtcggtcac gcgtgcctgc ggccactgct cgcgcgccag
     2821 ccgtcgcacg tcggtcagcg tgacgaggcc ggcgaggcca ccatcgtcgt gccgaaccag
     2881 gaaggatcgg tcctcgccgc gcagcagccg ctcctgcacc aggtcgccga ccgtctcgtt
     2941 gggtgagacg ctcggcggct cggcctccat tgcgtcgcgt acgcgcacgc cgcgcaggct
     3001 gcgctcgatc cccgcctggg ccgccgtcgc ctccgccgcg ttcgagagga accagccgat
     3061 gagcgccagc cacaggccgc cgaagatatt gccaggcccg agggcgaggt agacgccgaa
     3121 gccgatgagc aggtacgcga agagccgccc gaccatggcg gcgttgcggg tcgcgaccag
     3181 gcggtcgccg cggatgcgcc agatgagggc gcgcaggacg cggccgccgt ccatcgggaa
     3241 gccgggaagc aggttgaagg ccccgagcag gaggttgatc acgcccagcc actcggtcag
     3301 ggcccgaatc tgctcctggt cgaccacggc gccgatcccc agcagcacgc cgccgaccag
     3361 gaagctcgag accggaccgg ccagggcgat cagcgcctcc tctcgcggcg tgcgcgattc
     3421 gctgtcgatg gtcgtcgccc caccgaagat gaacagcgtg atgccctcga ccttcaggcc
     3481 gaagcgacgg gccagcagcg catggctcag ctcgtggacc aggaccgagc cgaagaagag
     3541 gagggccgtg atggcgccga tgccccagta cagcgcggtg gaccagccgc ggaactcgcg
     3601 cgggaacacc gactcggcca gcgagaaggt gaccaggaag aagatcacca gccagctggg
     3661 gtggatcgcg atgtcgatgc cgccgatgcg gccgattcgc cagccgggtc ccatgg
//
LOCUS       DASTYP010000114         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_299701, whole genome shotgun sequence.
ACCESSION   DASTYP010000114 DASTYP010000000
VERSION     DASTYP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299701"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VFV59_05325"
     CDS             <1..1291
                     /locus_tag="VFV59_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEU5325286.1"
                     /translation="VVSDRVHQLGEVLRTAREAKGVDLARVERDTKIRERYLSALERG
                     EYRELPGAVYTKGFLRNYGAYLGLDPEYLIDLYRIETGALTVERPAVAPRPLRGRRAR
                     ALVLTPNVVAAALLTVAVGGFIAYLAYEFVNFARQPELTITDPVGNVSGYTDDTITVR
                     GVTAPNARITVSGLPENPTVLADEEGNFRVTLNLLPGSNEFRLKARDPVTDRDSEEET
                     RVVVVESEVAASPSSSPAAITVTAPVADATVTSPVAVTGTSAPGATLTVTATLVSVAT
                     PTFSVTDAAGAAVALAPATPAPPAPTTLTADGSGAFNGTLPLAAGAWDVTVAPDTGEP
                     VTRRVTVGPAQGLVGALRLDGGDSYLEVDADGAPVPGVSGTIAAAGDRIDLTATRELR
                     IRAGNAGAVRVTINGIDIGAMGPAGAVVEWRISRSGG"
     gene            1329..1820
                     /locus_tag="VFV59_05330"
     CDS             1329..1820
                     /locus_tag="VFV59_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU5325287.1"
                     /translation="MAGESSFDVVSDFDQQELVNALDQARREIATRYDFKGSKVGFEL
                     GKESITLTADDEYRAGAAKDLLESKAVRRGLSLKIFDWGRIEPAAGGTVRQEIKLKRG
                     LTSDQAKELSKRIREQFPKTKPVIQGDAVRVSAKSKDDLQAVIADLRALDYPVALQFT
                     NYR"
     gene            1825..2340
                     /locus_tag="VFV59_05335"
     CDS             1825..2340
                     /locus_tag="VFV59_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEU5325288.1"
                     /translation="MPSPRPPTDDQLESAARRLGRALVKRGWQMAAAESCTGGLIGHV
                     ITQVPRSSDHFLGGIVSYSNAVKDSLLGVPEALIDELGAVSAEVAEGMAEGALRAFPV
                     ATIAVSVTGIAGPDGGSDEKPVGLTYVGAAIRDGRAVVERHQWPFDRDGNKRASALAA
                     LELAAQLVAEG"
     gene            2344..>3393
                     /locus_tag="VFV59_05340"
     CDS             2344..>3393
                     /locus_tag="VFV59_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5325289.1"
                     /translation="MVRASERIHVIGIAGSGAAGTAVLLAHAGAIVDGCDADAPSPYT
                     PPLDAAGVRYVNGHDPAHLAGVERVAITPALRAVPAHPELEAAVASGIPVVAWQALLG
                     ELMANPGRIGIGVAGTHGKSTTTAMLGHLLIGAGLNPTVEVGAFIDAWGASVRPGAGR
                     PFVVEADEFGDNFLNYHPGGAIVTNVEMDHPDYFADREAVMASFERFVRGMGGDPALG
                     GRLLVVAADNPGATELVGRLRGWQGRIIRYGPGGDIVATDVQRDERGTRFTLFDHPFA
                     MSLAGEHNVANATAALIMARTLGADLDRLAMALETFAGAGRRMELIADTPRVTIYDDY
                     GHHPTEVRAALSAARQ"
BASE COUNT          506 a         1211 c         1230 g          446 t
ORIGIN      
        1 tgtcgtgagc gaccgggtcc accagctcgg ggaggtgctg cgcaccgccc gcgaggcgaa
       61 gggcgtggac ctggcccggg tcgagcgaga cacgaagatc cgggagcggt acctctcggc
      121 gctggagcgg ggcgagtacc gcgagctccc cggcgcggtc tacaccaagg gattcctgcg
      181 caactacggc gcgtacctgg ggctcgaccc ggagtacctg atcgacctgt accgcatcga
      241 gaccggcgcg ctcaccgtcg agcgaccggc ggtggcgccg cgtccgctgc gcggccggcg
      301 cgcgcgggcc ctggtgctga cgccgaacgt cgtggccgcc gcgctcctga ccgtggcggt
      361 cggtggcttc atcgcctatc tcgcctacga gttcgtcaac ttcgcccgcc agccggagct
      421 gacgatcacc gacccggtgg gcaacgtcag cggatacacc gacgacacca tcaccgtccg
      481 cggcgtcact gctccgaacg cccggatcac cgtgagcggc ctgcccgaga acccgaccgt
      541 gctcgccgat gaggagggca acttccgcgt gacgctgaac ctcctcccgg gctcgaacga
      601 gttcaggctg aaggctcgcg acccggtcac cgaccgcgac tccgaggagg agacgcgggt
      661 cgtggtcgtc gagagcgagg ttgcggccag cccgtcctcg agccccgcgg cgatcacggt
      721 caccgccccg gtcgccgacg cgacggtgac gtcgccggtc gcggtcaccg gcacgagcgc
      781 gccaggagcc acgctgaccg tcaccgcgac gctcgtctcg gtcgcgacgc cgacgttcag
      841 cgtcaccgac gcggccggcg cggccgtggc cctggcaccc gcgacgccgg ccccaccggc
      901 cccgacgacg ctgacggcgg acggcagcgg cgccttcaac ggaacgctgc cgctcgcagc
      961 cggcgcatgg gacgtgacgg ttgcccccga caccggcgag ccggtgacgc ggcgcgtgac
     1021 cgtcggaccc gcgcagggcc tcgtgggggc gctgcggctc gacggcgggg actcgtacct
     1081 cgaggtcgat gccgacggcg cgccggtgcc cggcgtgtca ggcaccattg cggcagctgg
     1141 cgaccgcatc gacctcacgg caacgcgcga gctgcgcatc cgggccggca acgccggcgc
     1201 cgtgcgcgtc acgatcaacg gcatcgacat cggcgcgatg ggaccggccg gagccgtcgt
     1261 ggagtggcgc atcagccgct ccggcggctg agccggagcg catcggtcag cgaaggaggc
     1321 agacgacctt ggcaggagag tcatcgttcg acgtggtgag cgacttcgac cagcaggagc
     1381 tggtcaatgc gctggaccag gcgcggcgcg agatcgcgac gcgctacgac ttcaagggca
     1441 gcaaggtcgg cttcgagctg ggcaaggaat cgatcacgct caccgccgac gacgagtacc
     1501 gcgccggggc cgcgaaggac ctgctggagt cgaaggccgt tcgccgcggc ctgagcctga
     1561 agatcttcga ttggggcagg atcgagcccg cggccggcgg gacggttcgg caggagatca
     1621 agctcaagcg tggcctcacc agcgaccagg ccaaggagct ctcgaagcgc atccgcgaac
     1681 agttccccaa gaccaagccg gtgatccagg gcgacgccgt ccgcgtcagc gcgaagtcga
     1741 aggacgacct gcaggcggtc atcgcggacc tccgcgcgct cgactacccg gtcgcgctcc
     1801 aattcacgaa ctaccgctag accaatgccg tcgccgcgcc cgcccacgga cgaccagctc
     1861 gagtccgccg ctcggcggct gggccgagcg ctcgtcaagc gtggctggca gatggccgcc
     1921 gccgagtcgt gcaccggcgg gctcatcgga cacgtcatca cgcaggtgcc caggtcgagc
     1981 gaccacttcc tgggtgggat cgtcagctac tccaacgcgg tgaaggactc gctgctgggc
     2041 gttcccgagg cgctgatcga cgagctcggc gccgtctccg ccgaggtggc cgagggcatg
     2101 gccgagggtg cgctgcgcgc gttcccggtc gccacgatcg ccgtctcggt caccggcatc
     2161 gccggacccg acgggggatc ggacgagaag ccggtcggcc tgacctacgt cggcgccgcc
     2221 atccgcgacg gccgcgccgt ggtggagcgg caccagtggc cgttcgaccg tgacggcaac
     2281 aagcgcgcgt cggcgctggc cgccctggag ctggcggcgc agctcgtggc cgagggctga
     2341 ccgatggtgc gcgccagcga gcggatccac gtcatcggga tcgcgggatc cggggcggct
     2401 ggcacggccg tgctgctcgc ccatgccggg gccatcgtgg atggctgtga cgcggatgcc
     2461 ccgtcgccgt acaccccacc gctcgacgcc gcgggagtcc gctacgtcaa cggccatgac
     2521 cccgcccacc tcgccggtgt cgagcgcgtg gcgatcaccc ccgcgctgcg cgccgtcccg
     2581 gcccacccgg agctggaggc cgcggtcgcg tcgggcatcc cggtggtcgc ctggcaggcg
     2641 ctcctcgggg agctcatggc gaaccccggc aggatcggca tcggggtggc cggaacgcat
     2701 ggcaagtcca ccaccacggc gatgctcggc cacctgctga tcggcgcggg actcaacccc
     2761 acggtggagg tcggggcgtt catcgacgcg tggggcgcat cggttcggcc tggagccggt
     2821 cgtcccttcg tggtggaggc ggacgagttc ggcgacaact tcctgaacta ccaccctggc
     2881 ggcgcgatcg tcaccaacgt cgagatggat caccctgact acttcgcgga tcgcgaggcg
     2941 gtgatggcct cgttcgagcg gttcgtgcgg ggcatggggg gcgatccggc gctcggcggg
     3001 cggctgctgg tcgtcgccgc cgacaacccg ggcgccactg agctggtcgg gcgtctgcgc
     3061 ggctggcagg gccgcatcat caggtacggg ccggggggcg acatcgtcgc caccgacgtc
     3121 cagcgcgacg agcgcggcac ccggttcacc ctgttcgacc atccgttcgc catgtcactg
     3181 gccggggagc acaacgtggc caacgcgacg gccgcactga tcatggcgcg cacgctgggc
     3241 gcggacctcg atcggctggc gatggcgctc gaaacgttcg cgggagccgg gcggcgaatg
     3301 gagctgatcg ccgatactcc gcgcgtgacg atctacgacg actacggcca ccatcccacc
     3361 gaggtgcgcg cggcgctctc cgccgctcgc cag
//
LOCUS       DASTYP010000115        10974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1429253, whole genome shotgun sequence.
ACCESSION   DASTYP010000115 DASTYP010000000
VERSION     DASTYP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429253"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="pyrD"
                     /locus_tag="VFV59_05345"
     CDS             <1..721
                     /gene="pyrD"
                     /locus_tag="VFV59_05345"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEU5325290.1"
                     /translation="TVTPHPQPGNPRPRLFRLPPDEALVNRMGFNNAGADALARRIAE
                     ARPDLPPGFVVGANIGRNRDTPDERAVEDYAAAAAVLGPVADYLAINVSSPNTQGLRN
                     LQDPVRLPEIVAAVAGAAPSHPIFVKLAPELDVERRRAILEALVDGPTAGVILSNTTV
                     QRDGLRGAAAAEQGGVSGRPLVGRTGEVLAEVRSATGDRFVIVASGGLERRPAEADLV
                     ELWTAMIYRGPGVIGAAARTG"
     gene            complement(728..1174)
                     /locus_tag="VFV59_05350"
     CDS             complement(728..1174)
                     /locus_tag="VFV59_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5325291.1"
                     /translation="MTMSEPIAAIRKELILPIAPDRVFRLFTADIDRWWPLATHSVEG
                     EDAAGVSIDGRVGGLIAEASTTGRRHVWGTIQLWDPPRRFVTTWHPGRPADAPTELEV
                     RFEPWEDGTRLVLEHRGWEHAAWIGDRSNYESGWDPVLARFVETVR"
     gene            complement(1161..1475)
                     /locus_tag="VFV59_05355"
     CDS             complement(1161..1475)
                     /locus_tag="VFV59_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5325292.1"
                     /translation="MRYERALDVLGDPMRRRIMELLRPGPLPVGAIAERLPVSRPAVS
                     RHLRLLTDASLVRHRAHGTRNLYEVDRRGLEEVRAWLDAWWDEPLDRFADHVEEHSDD
                     DV"
     gene            1618..2988
                     /locus_tag="VFV59_05360"
     CDS             1618..2988
                     /locus_tag="VFV59_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017317233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEU5325293.1"
                     /translation="MTTMEATPSRAAATEQAAPHIVTELPGPKARALIERDEAVASPS
                     LTRAYPLVAESGAGAVITDVDGNRFLDFAAGIAVCSTGHAHPKVVSAIKEQADRLIHV
                     AATDFYEPRYVDFMERLADIAPFEEKARVFVTNSGTEAVEGAIKLARYHTHRPGIIAF
                     EGAFHGRTMGALSLTNSKIKQRAGFGPLIPMVHHAPFPRVRSWKEGSGGDGTAELEVL
                     RRSILGRIIAPTDVAAIVIEPIQGEGGYFPAPAAFLRGLREICDEHGILLVADEIQSG
                     MGRTGTWWAIEHAGVEPDIVTTAKGIASGMPIGAFIARDSVWTWPPGAHGSTFAGNPI
                     CAVAGLATLDIIESEAMANAAAMGDRLRAGLERVADDIAEVRDIRGIGLMLGVEFSDH
                     AHAEAVQEAAFQRGLLTLECGESSLRFSPPLIVSEAEVDRAIEIVGEALRELSHPRAG
                     IAETGG"
     gene            complement(3062..4192)
                     /locus_tag="VFV59_05365"
     CDS             complement(3062..4192)
                     /locus_tag="VFV59_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5325294.1"
                     /translation="MRTVAAGGLVAVLLLLAACSAPVASPSTSGPSASPTASGHPSAS
                     PSPSPTATPSAEQPTPSASVGGTTYTVQPGDGLMAIARAFDTTADQLVAWNVDRYPSL
                     ATDPNVLEPGWILVVSGDPDVTPRATPSPTPRATLPPTATGCTAGNRADTGAAETFRT
                     IPDAGAEVALTFDMGGRMDPAVDILEFLVEHEVCATIFATGAMSQTAQGQAALAIVRD
                     HPELFEMGNHTMYHCDLVRGGGGSPSSQPCVGGAPSADRIRQELTDAEAILRDATGQD
                     PQPYWRPPYGSINDAVLGAAASVGYTKTFLWDIDSVDWKPISDGGPTAAQIASKVVGN
                     AVNGSNVLFHLGGYETLDALELLVPALRDRGFVLTSLSDLLD"
     gene            complement(4198..4911)
                     /locus_tag="VFV59_05370"
     CDS             complement(4198..4911)
                     /locus_tag="VFV59_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU5325295.1"
                     /translation="MRLPYFPLHQVLFPHLPLPLHVFEERYRALARDVVAEGSPFGGR
                     FVVSMITEGREVGGDAATQPIGTICEVHSAEQFPDGRWLLLVVGVARARLGAVDRSGP
                     YPLVEAHELPEREGDGATSALPAVQQALDAYLDTVKRFVAATASGASDPREPRDVTTT
                     LDQVLKPIHLPDDPSAASYAVAGVLQVELSRKQHLLELPDAATRLRAELEMLRRESRL
                     LGDGATAPVPAADLGYHPN"
     gene            complement(4998..6497)
                     /locus_tag="VFV59_05375"
     CDS             complement(4998..6497)
                     /locus_tag="VFV59_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5325296.1"
                     /translation="MTTTYQNLIGGEWKPAASGRTFTSVSPANHEELVGEFPASGAED
                     VEAAVAAAKAAYPSWSLMPAPKRGEILFRVARLLAEHKEELSRLMTREMGKVLAEARG
                     DVQEAIDVAYYMAGEGRRLFGQTVPSEMPDKFAMAIRRPIGVVGIITPWNFPVAIPAW
                     KLFPALICGNTAVMKPASDTPACMVRFVELLVEGGIPAGVVNVVTGSGGEVGNAIVDH
                     PDVRVISFTGHTDTGVEISTRAAKTLKRVSLELGGKNPIVIWEDADLDLALDSVVWSA
                     FGTSGQRCTAASRLIVHRNVHDDFVHRLQQRVAKLVLGDGLEETTDVGPVINDRAVER
                     IAEYATIGRSEADLVIGGEPAREGALAKGSFFQPTIFTEVKPDARIAQEEIFGPVASV
                     IPVTTWEETVEVVNGVKYGLSTSLFTHDINLAFRSIRDFDSGLGYVNHGTIGAEAHLP
                     FGGIKATGNGHREVGQAALDFFSEWKSVYIDYSGKLQRAQIDTTFVDQD"
     gene            6875..7840
                     /locus_tag="VFV59_05380"
     CDS             6875..7840
                     /locus_tag="VFV59_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325297.1"
                     /translation="MVRRHPTLVPLLLAVLLMTCTAVPVLGAKPSQPGKPAPSSPPTS
                     PPPTLEEPPLTPEQEAASAAKVASAQAYLASVDAGGLELSNLNCVTPTGTAEVTATTD
                     AAGGGRSADGPGTDATIDACWVPQGFVGVEARDQIGGIYCGPAVGQVISNYTWAMGSA
                     WNKYTQDVIAGWMSTVINQGTSAEKLEVGLERATIGAPRRPSNWDWVITYLRDLNGNG
                     DIADEMHGYIRTNVSSSKMPLAIPVKPHDPNSTYYLSSWPKPVWSPGHWIAAYGWVGY
                     YTGTDYARTYYTDSSKDEGGSTGKFWDPTRHIARLIAQHTGRFVW"
     gene            8044..9087
                     /locus_tag="VFV59_05385"
     CDS             8044..9087
                     /locus_tag="VFV59_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325298.1"
                     /translation="MHPLDLAWTSPILEFISDGTAVVFSSGVADGPDGDTAPDLWRYT
                     PGEAAPELVWSNPKRDRQVIRIAGDAGTLAWVDMDARGRPDWNLWLLPRHESEPLLLD
                     VHPGDAAVPSLVPSMAVSEDVIAWTAFDAGAHGAVSQLLVAQAPDWRPRLLLEREAAE
                     AELWLPSVRGTSLAFTEVRYADDRTTDERFVYLTDATDPSSLTRLDESARATMPLILP
                     GGDVVWKETDPGFNMFNWGELVRWDHETGAIRPMATRWQEYVNYPSVGERFVAAWGSD
                     SFTFAVYDAELAVWRLIDRYPLEQHVAVPRVHVGGPLIAWVVIPTLDSDPTPPQLYWA
                     YLPGAGTSTKPPP"
     gene            complement(9100..10485)
                     /locus_tag="VFV59_05390"
     CDS             complement(9100..10485)
                     /locus_tag="VFV59_05390"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEU5325299.1"
                     /translation="MTEYRIEHDSMGEVRVPADAKWRAQTQRAIENFPISGMRLERAQ
                     IQALARIKAAAAAVKLELGMIDADLAQAIIGAAEEVARGEHDHEFPLDVFQTGSGTSS
                     NMNMNEVLATLAAERLGRPVHPNDDVNHPMSSNDMFPASIHIAATSGVVNDLVPALEH
                     LAESLERKAGEFAEVVKSGRTHLMDATPVTLGQEFGGYARQVRLGVERLHATLPRVAE
                     LPLGGTAVGTGINSPAGFGGRVIAEVASATGLPLTEAVDHFEAQGARDGLVELSGQLR
                     TIAVGLTKIANDVRWMGSGPRAGLGEINLPDLQPGSSIMPGKVNPVLPEATLMVCAQV
                     IGNDAAVAWGGAAGNFELNVMLPMLGRNLLESIRLLSNVTRLLADRCVDGITANVERL
                     REYAESSPSIVTPLNRYLGYEEAASIAKQALKERKTIRQVVVERGHVEAGRLTEQQLD
                     EALDVMRMTHP"
     gene            complement(10482..10946)
                     /locus_tag="VFV59_05395"
     CDS             complement(10482..10946)
                     /locus_tag="VFV59_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEU5325300.1"
                     /translation="MTDLRSAGPARTDPGTAGEQPAGRTAPLDPREAERLFRRGVALF
                     NGVRYWHAHEAWETLWRAAPDEERDFYQGLIQVAAGLLHLQRKNLRGARNKLGEGLDR
                     LEPYQPVHNGVLVNELIGKGRRVLDDLNAGYLPYLIPPVIRFVDAEEPDFSR"
BASE COUNT         1581 a         3852 c         3920 g         1621 t
ORIGIN      
        1 gacggtgacc ccgcatccgc agccgggcaa tccccgcccc cgcctcttcc ggcttccgcc
       61 cgacgaggcc ctggtcaacc gcatgggctt caacaacgcc ggagccgatg cgctggcgcg
      121 ccggatcgcc gaggcccggc ccgacctgcc gcccggcttc gtggtcggcg cgaacatcgg
      181 ccgcaaccgg gacacgccag acgaacgggc cgtggaggac tacgcggcgg ccgcggcggt
      241 gctggggccg gtggcggact acctggcgat caacgtcagc agcccgaaca cgcaggggct
      301 gcgcaacctg caggaccccg tccgcctgcc cgagatcgtg gcggcggtcg cgggcgcggc
      361 accgtcccac ccgatcttcg tcaagctggc gcccgagctc gatgtcgagc ggcgccgcgc
      421 catcctcgag gcgctggtcg acgggccgac cgcgggggtg atcctgtcga acaccactgt
      481 gcagcgcgac gggctgcgcg gagcggcggc ggccgagcag ggaggtgtct caggacggcc
      541 gctggtgggg cggaccggcg aggtcctggc ggaggtgcgc tcagcgaccg gggaccgatt
      601 cgtcatcgtc gcgtccggtg gcctggagcg ccggccggcc gaggccgacc tggtggagct
      661 gtggaccgcg atgatctacc gcggcccggg cgtcatcggc gcggccgcac ggaccgggta
      721 gcggcgctca gcggacggtc tcgacgaacc gcgcgaggac cggatcccag cccgactcgt
      781 agttggagcg gtcgccgatc cacgccgcgt gctcccagcc gcggtgctcg aggacgaggc
      841 gcgtcccgtc ctcccacggc tcgaagcgca cctcgagctc ggtcggcgcg tcggccggcc
      901 ggccgggatg ccaggtcgtc acgaagcggc gcggcgggtc ccacagctgg atggtgcccc
      961 agacgtgccg ccgtcccgtc gtgctcgcct cggcgatcaa cccaccgaca cgaccgtcga
     1021 tgctcacccc ggcggcatcc tcgccctcca ccgagtgcgt cgccagcggc caccatcggt
     1081 cgatgtcggc ggtgaagagg cggaaaacgc gatccggcgc gatcggcaag atcagctcct
     1141 tgcgaatggc ggcgatcggc tcagacatcg tcatcggaat gctcctccac gtggtcggcg
     1201 aatcggtcga gcggctcgtc ccaccaggcg tcgagccagg cgcggacctc ctcgaggccg
     1261 cgccgatcga cctcgtacag gttgcgggtg ccgtgcgccc ggtgccggac cagcgacgca
     1321 tcggtcagca ggcgaaggtg ccgcgacacg gccgggcggc tcaccgggag tcgctcggca
     1381 atggcgccaa ccggcagcgg tccgggacgg agaagctcca tgatccgccg tcgcatcggg
     1441 tcgccgagga cgtcaagcgc ccgttcgtaa cgcattggtt acggtaactt acgggttacg
     1501 caacgtcgtg tcaagcccgg tgctggctac cgagggcgcg tcggcgctgg tagcatggca
     1561 tcgcacttag ccaggggcgc gggcaggacg cgccgctccg tttcggagtg acccctcatg
     1621 accaccatgg aggcaacccc ctcgcgtgcg gctgccaccg agcaggccgc gccacacatc
     1681 gtcaccgagc tgccgggccc gaaggcccga gcgctgatcg agcgggacga ggccgtcgcg
     1741 agcccctcgc tgacgcgcgc ctacccgctc gtcgcggagt ccggcgccgg cgccgtcatc
     1801 acggacgtcg acggcaaccg cttcctcgac ttcgccgccg gcatcgccgt gtgctccacc
     1861 ggccacgcgc acccgaaggt ggtgtcggcc atcaaggagc aggctgatcg cctcatccac
     1921 gtcgcggcga cggacttcta cgagccccgc tacgtcgact tcatggagcg gctggcggac
     1981 atcgcgccgt tcgaggagaa ggcacgcgtc ttcgtcacca actcggggac cgaggccgtc
     2041 gagggcgcca tcaagctggc gcgctaccac acccatcggc ccgggatcat cgccttcgag
     2101 ggcgccttcc acggccggac catgggcgcg ctctcgctca ccaactcgaa gatcaagcag
     2161 cgcgccggct tcggaccgct gatcccgatg gtgcaccacg cccccttccc gcgcgttcgg
     2221 tcgtggaagg agggctccgg cggtgacggt acggccgagc tcgaggtgct gcgccgttcg
     2281 atcctgggcc ggatcatcgc gccgaccgac gtggccgcga tcgtcatcga gccgatccag
     2341 ggcgagggcg gctacttccc ggcgccggcc gccttcctcc gcgggctgcg cgagatctgc
     2401 gacgagcacg gcatcctgct ggtcgccgac gagatccagt ccggcatggg ccgcaccggc
     2461 acgtggtggg cgatcgagca cgccggcgtc gagccggaca tcgtcaccac ggccaagggc
     2521 atcgcctcgg gcatgccgat cggcgcgttc atcgcgcgcg actcggtctg gacctggccg
     2581 cccggggccc acggctcgac cttcgccggc aacccgatct gcgccgtcgc cggcctggcg
     2641 acgctggaca tcatcgagtc cgaggcgatg gccaacgcgg cggccatggg cgaccggctc
     2701 cgcgccggcc tcgagcgggt cgccgacgac atcgccgagg tgcgcgacat ccggggcatc
     2761 ggcctgatgc tcggggtcga gttctcggac cacgcccacg ccgaggcggt gcaggaggcg
     2821 gcgttccagc gcggcctgct gaccctcgag tgcggcgagt cgtcgctgcg cttcagcccg
     2881 ccgctgatcg tctccgaggc cgaggtggac cgggcgatcg agatcgtggg cgaggcgctc
     2941 cgcgagctgt cgcacccgcg ggcgggaatc gccgagaccg gcggctgacc cacctccgca
     3001 accgatccgg aagcgccgct cctcgatcgg ggagcggcgc ttcgacgtcc cggggccgcg
     3061 gtcagtcgag caggtcggag aggctcgtca gcacgaagcc acggtcccgc agcgcgggga
     3121 cgaggagctc gagcgcatcg agcgtctcgt agccgcccag gtggaacagc acgttcgacc
     3181 cgttcaccgc gttgccgacc accttcgagg cgatctgtgc ggccgtcggc ccgccgtcgc
     3241 tgatgggctt ccagtcgacg gagtcgatgt cccacaggaa ggtcttggtg tacccgaccg
     3301 aggcggccgc acccaggacg gcgtcgttga tcgatccgta gggtggccgc cagtagggct
     3361 gcgggtcctg gccggtcgcg tcgcgcagga tggcctccgc atcggtcagc tcctggcgaa
     3421 tccgatcggc gctcggggcg ccgccgacgc acggctggct cgagggcgat ccgccacccc
     3481 cacgcacgag gtcgcagtga tacatcgtgt ggttgcccat ctcgaacagt tcaggatggt
     3541 cgcgcacgat ggccagggcc gcctggccct gcgccgtctg gctcatggcg ccggtggcga
     3601 agatggtggc gcacacctcg tgctcgacca ggaactcgag gatgtcgacg gccgggtcca
     3661 tgcgcccacc catgtcgaag gtcagcgcga cctcggcgcc ggcgtccggg atcgtccgga
     3721 acgtctccgc cgccccggtg tcggcgcggt tgccggcggt gcagcccgtg gccgtcggcg
     3781 gcagcgtggc gcgaggggtg ggtgacggcg tcgctcgcgg cgtgacgtcg ggatcaccac
     3841 tgacgaccag gatccagccg ggttcgagga cgttcgggtc cgtcgccagc gaggggtagc
     3901 ggtccacgtt ccacgccacc agctggtcgg ccgtcgtgtc gaaggcgcga gcgatggcca
     3961 tgaggccgtc gccgggctgc accgtgtacg tggtgccgcc gaccgatgcg ctcggcgtcg
     4021 gctgctcggc gctcggcgtg gccgtcggtg acggtgacgg tgaggccgag gggtggccgc
     4081 tcgccgtcgg cgacgcggag ggcccggacg tcgaaggcga ggccacgggg gcggagcacg
     4141 cggcgaggag gaggagcacg gcgaccaggc ccccggcggc gacggttcgc atgcgcgtca
     4201 gttcgggtgg tagccgagat cggcggccgg gaccggcgcc gtggcgccgt cgccgagcag
     4261 tcgagattcg cggcggagca tctcgagctc ggcgcgaagg cgcgtggccg catcgggcag
     4321 ctccagcagg tgctgcttgc ggctgagctc gacctggagg acaccggcga cggcgtagct
     4381 ggccgccgac gggtcgtcgg gaaggtggat gggcttgagg acctggtcca gggtggtcgt
     4441 cacgtctcgt ggctctcgcg ggtcactcgc tccagacgcg gtggcggcca cgaaacgttt
     4501 cacggtgtcg aggtaggcgt cgagcgcctg ctgcacagcg ggcagcgcgg acgtggctcc
     4561 gtcaccctcg cgttccggca gctcatgggc ctccacgagc gggtagggcc cgctgcggtc
     4621 caccgctccc agccgcgccc gggcgacgcc gacgaccagg agcagccacc ggccgtccgg
     4681 gaactgctcc gcgctgtgca cctcgcagat ggtgccgatc ggctgggtcg cggcgtcgcc
     4741 gccgacctcg cggccctcgg tgatcatcga aaccacgaag cgcccgccga acggggagcc
     4801 ctcggccacc acgtcgcggg ccagcgcccg gtaccgctcc tcgaagacgt ggagcgggag
     4861 tgggaggtgc gggaagagca cctggtgcag cgggaagtac gggaggcgca tgctgcggcg
     4921 catggtcgca ccgggcgtgc cacccggcaa ccgcggcgtg aggcggcgcc gcgggagggc
     4981 ccgcggcgca tcggtcctca atcctggtcg acgaaggtcg tgtcgatctg ggcgcgctgc
     5041 agcttgccgg agtagtcgat gtagacgctc ttccactcgc tgaagaagtc gagggccgcc
     5101 tggcccacct cgcggtggcc gttgccggtg gccttgatgc cgccgaacgg caggtgcgcc
     5161 tcggcgccga tggtgccgtg gttcacgtac cccaggcccg agtcgaagtc ccggatgctg
     5221 cggaaggcca ggttgatgtc gtgcgtgaac agcgaggtcg acaggccgta cttcacgccg
     5281 ttgacgacct cgacggtctc ctcccacgtc gtcaccggga tgaccgatgc gaccggcccg
     5341 aagatctcct cctgcgcgat gcgggcatcc ggcttgacct ccgtgaagat ggtcggctgg
     5401 aagaacgagc ccttcgcgag ggccccctcg cgcgccggct cgccgccgat gaccaggtcg
     5461 gcctcgctgc ggccgatggt cgcgtactcc gcgatccgct ccaccgcgcg atcgttgatg
     5521 accgggccca cgtcggtggt ctcctccaga ccgtccccca gcacgagctt ggcgacccgc
     5581 tgctgcagcc gatgcacgaa gtcgtcgtgg acattgcgat gcacgatgag gcgcgaggcg
     5641 gccgtgcagc gctggccgga ggtgccgaag gccgaccaca ccacgctgtc gagcgccagg
     5701 tcgaggtccg catcctccca gatgacgatc gggttcttcc caccgagctc caggctgacg
     5761 cgcttcaggg tctttgccgc gcgcgtgctg atctcgacgc ccgtatcggt gtggccggtg
     5821 aagctgatga cccgcacgtc ggggtggtcg acaatggcgt tgccgacctc gccgcccgac
     5881 cccgtgacga cgttgacgac cccggccggg atgccgccct cgaccagcag ctcgacgaag
     5941 cggaccatgc aggccggcgt gtcggatgcg ggcttcatga ccgcggtgtt gccgcagatc
     6001 agcgccggga agagcttcca agccgggatg gcgaccggga agttccacgg ggtgatgatc
     6061 ccgaccacgc cgatgggccg gcggatggcc atggcgaact tgtcgggcat ctccgagggg
     6121 acggtctgac caaagaggcg ccggccctcg ccggccatgt agtaggcgac gtcgatcgcc
     6181 tcctggacat ctccccgcgc ctccgccagc accttgccca tctcgcgcgt catgaggcgg
     6241 ctgagctcct ctttgtgctc ggccaggagc cgggccacac ggaagaggat ctcgccgcgc
     6301 ttcggggccg gcatcaggga ccagctcggg taggccgcct tcgcggcggc caccgcggcc
     6361 tcgacgtcct cggctccgga ggccgggaac tcgcccacca gctcctcgtg gttggccggg
     6421 ctgacgctgg tgaaggtgcg gcccgatgcg gccggcttcc actcgccgcc gatcaggttc
     6481 tggtacgtgg tcgtcacggg aaggtcatct ccttgggcgc gcggagcgat gcgcgcgagt
     6541 gtgttcgggc ctcgattcta tccctgcccc gcagggacat ctgcccctac acctcggcgc
     6601 ggcgctgggc gaggatgagg ccacaattga agaaccgtcg cgtcgggttc ctgttgttca
     6661 acgataacgg ggctgaagta gtggcacccc ggcgtgatgg ctcagcgagg cgccgatccc
     6721 atccgggatc ggcgttttcg tatgcctgct catggccgac gcggccagta atcgggcagt
     6781 aagcggccga gaagagactg tgcggcatca tggccgccga tacgacgccc tcgcccacgc
     6841 cggctcgcct ccatggtcta ggaggctgcc cccgatggtc cgcagacacc caaccctcgt
     6901 tcccctgctc ctcgccgtgc ttctgatgac gtgcaccgcc gttcccgtcc tcggcgcgaa
     6961 gccgagccag ccgggcaagc cggcgcccag ctcgcccccg accagccctc ccccgaccct
     7021 ggaggagccg ccgctcacgc ccgagcagga ggcggcatcc gccgcgaagg tcgcctccgc
     7081 ccaggcatac ctcgcctcgg tggatgccgg cggcctcgag ctgtcgaacc tgaactgcgt
     7141 cacgccgacc gggaccgctg aggtgaccgc cacgacggat gccgcgggcg gcggtcgctc
     7201 cgcagacggt ccgggcaccg acgccacgat cgacgcctgc tgggtgccgc agggctttgt
     7261 gggcgtggag gcccgggacc agatcggtgg catctactgc gggcccgccg tcgggcaggt
     7321 catctcgaac tacacctggg ccatggggtc ggcgtggaac aagtacacgc aggacgtgat
     7381 cgccggctgg atgtcgacgg tgatcaacca gggcacctct gcggagaagc tcgaagtcgg
     7441 gctcgagcgc gccaccatcg gcgctccgcg ccgtccgagt aactgggact gggtgatcac
     7501 ctacctgcgc gacctcaacg gcaatggcga catcgccgat gagatgcacg ggtacatccg
     7561 cacgaacgtg agcagctcga agatgccgct ggccatcccg gtcaagcccc acgaccccaa
     7621 ctccacgtac tacctgtcca gctggccgaa gccggtgtgg tccccgggcc actggatcgc
     7681 ggcgtacggc tgggtcgggt actacaccgg cacggactac gcccgcacgt actacaccga
     7741 ctcctcgaag gacgaggggg gctcgaccgg gaagttctgg gatccgacgc gtcacatcgc
     7801 tcgtctgatc gcgcagcaca ccgggcggtt cgtgtggtga gacgaccgtg cgcagcgctc
     7861 acggcgcttg gcgttgcggc gatgggggtg acggcctgcg gggccgtcac ccccatcccg
     7921 tccgaggcat ctccatcggc cgtggtggtt gcgacgccag agcccacccc aggggttgcc
     7981 tccagcgccc cgccaccgac gccgagaaca cggcccctgg gggacctcgg acggcagatc
     8041 gagatgcacc cgctcgacct ggcgtggacc agcccgatcc tcgagttcat cagcgacggc
     8101 accgcggtcg tcttctcctc gggcgtggcc gacggacccg atggcgacac cgcgccagac
     8161 ctgtggcgct acaccccggg ggaggcggcg ccagagctgg tgtggtcgaa cccgaagcgc
     8221 gatcggcagg tgatccgcat cgccggcgac gccggaacgc ttgcctgggt cgacatggac
     8281 gcaaggggtc ggccggactg gaacctctgg ctccttccgc gtcacgaatc ggagccgctc
     8341 ctgctcgatg tgcatccggg agacgctgcc gtcccgagcc tggtgccgag catggcagtc
     8401 tccgaggacg tcattgcctg gaccgcattc gatgcgggcg cgcatggtgc agtctcgcag
     8461 ctgctcgttg cacaggcacc ggactggcgg ccgcggcttc tgctcgagcg cgaggccgcg
     8521 gaggccgagc tctggctgcc gtcggttcgc ggcacgtccc tcgcgttcac cgaagtccgg
     8581 tacgccgacg accggaccac ggatgagcgg ttcgtgtacc tcaccgatgc gaccgatcct
     8641 tcctcgctca cgcgcctcga cgagagtgcg cgggcgacca tgccgctgat cctccccggc
     8701 ggcgatgtcg tgtggaagga gaccgatccg ggcttcaaca tgttcaattg gggagagctc
     8761 gtgcggtggg accacgagac gggcgcgatc cggccgatgg cgacccgctg gcaggagtac
     8821 gtgaactacc cgtccgtcgg cgagcgcttc gtcgccgcgt ggggatcgga ctccttcacg
     8881 ttcgccgtct acgacgcgga gctggcggtc tggcgcctca tcgatcgata cccgctcgag
     8941 cagcacgtcg ccgtgccgcg ggtccacgtc ggcggtccgt tgatagcgtg ggtcgtgatc
     9001 cccacgctgg attccgaccc gacgccgccg cagctgtact gggcatacct gccgggcgcg
     9061 ggaacgagca ccaagccgcc gccataggcg cggcgaacgt cagggatgcg tcatccgcat
     9121 gacgtccagg gcctcgtcca gctgctgctc ggtgaggcgc ccggcctcga cgtggccgcg
     9181 ctcgacgacg acctggcgaa tggtcttgcg ctccttgagc gcctgcttgg cgatgctcgc
     9241 cgcctcctcg tacccgaggt agcggttgag tggcgtgacg atcgatggtg agctctccgc
     9301 gtactcgcgc aggcgctcga cgtttgcggt gatcccgtcg acgcaccgat ccgcgaggag
     9361 ccgggtgacg ttcgacagca gccggatgga ctcgagcagg ttgcggccga gcatcggcag
     9421 catgacgttc agctcgaagt tgccggccgc gccgccccac gcgaccgcgg cgtcgttgcc
     9481 gatgacctgc gcgcagacca tcagcgtcgc ctcggggagg accgggttca ccttgccagg
     9541 catgatcgac gagccgggct gcaggtcggg aaggttgatc tcccccaggc cggcacgcgg
     9601 gccggatccc atccatcgca cgtcgttggc gatcttggtg aggcccaccg cgatggtccg
     9661 caattgcccc gacagctcga ccagaccatc ccgcgcgccc tgggcctcga agtgatcgac
     9721 ggcctcggtc agtggcaggc cggtcgcgct cgccacctcc gcgatgacgc ggccgccgaa
     9781 gccggccggt gagttgatgc cggtcccgac ggcggtgccg cccaacggca gctccgcgac
     9841 ccgcggcagc gtggcgtgga ggcgctcgac gccgagccgc acctgccggg cgtacccgcc
     9901 gaactcctgc ccgagcgtca ccggggtcgc gtccatgagg tgggtgcgcc ccgacttcac
     9961 gacctcggcg aactcgccgg ccttgcgctc gagggactcc gccaggtgct cgagcgcggg
    10021 cacgaggtcg ttgacgaccc ccgacgtcgc cgcgatgtgg atcgaggccg ggaacatgtc
    10081 gttcgacgac atcggatggt tgacgtcatc gttggggtgg accggccgac cgagacgctc
    10141 ggcggcgagc gttgcgagga cctcgttcat gttcatgttg gagctggtgc ccgagccagt
    10201 ctggaagacg tcgagcggga actcgtggtc gtgctcgccg cgggcgacct cctccgcggc
    10261 gccgatgatc gcctgcgcca gatccgcgtc gatcatcccg agctcgagct tgacggccgc
    10321 ggcggcggcc ttgatgcgcg ccagggcctg gatctgcgcg cgctccagcc gcatgccgga
    10381 gatcgggaag ttctcgatcg cgcgctgggt ctgggcgcgc cacttggcat cggcgggaac
    10441 gcggacctcg cccatcgagt cgtgctcgat gcggtactcg gtcatcggct gaagtccggc
    10501 tcctccgcgt cgacgaatcg gatcaccggg gggatgaggt atggcaggta gccggcgttg
    10561 aggtcgtcca ggacgcgccg gcccttcccg atcagctcgt tgaccagcac gccgttgtgc
    10621 accggctgat acggctcgag cctgtccagg ccctcgccga gcttgttccg cgccccgcgc
    10681 aggttcttgc gctgcaggtg cagcagtccg gcggcgacct ggatcaggcc ctggtagaag
    10741 tcccgctcct cgtccggcgc ggcccgccac agcgtctccc atgcctcgtg cgcgtgccag
    10801 tagcggacgc cgttgaagag ggcgacgccc cgacgaaaca ggcgctcggc ttcacgggga
    10861 tcgagcggcg cagtccgccc ggcgggttgc tcgcccgccg ttccgggatc ggtccgggca
    10921 gggccggcgc tcctgaggtc cgtcacgccc cgatcgtagc aggggcaggt ggaa
//
LOCUS       DASTYP010000116        11491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_8163, whole genome shotgun sequence.
ACCESSION   DASTYP010000116 DASTYP010000000
VERSION     DASTYP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8163"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFV59_05400"
     CDS             complement(<1..359)
                     /locus_tag="VFV59_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEU5325301.1"
                     /translation="MTVRRIDGGVTAPSGFRVAAARAGLRSGMGPDVMLLVSERGPVP
                     AAATFTRNRLRAAPVEVSMRSLRSSAGHAAAIVANAGCANAGTGTPGLADARAVQHLV
                     ADRLGVADRFVLTASTGL"
     gene            complement(356..1381)
                     /gene="argC"
                     /locus_tag="VFV59_05405"
     CDS             complement(356..1381)
                     /gene="argC"
                     /locus_tag="VFV59_05405"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5325302.1"
                     /translation="MNAPVRVAVVGASGYAGAELVRLLAGHPDVTIHTLYARGRDATP
                     MAEEYPHTAPLGVRLEDGAPEPGSIDVAFLALPHGASAELSGRLANGGTTVIDIGSDL
                     RLRDPAAYPDWYGFDHPAPDVLADAVYGLTEFARDRLRGARLIANPGCYPTASLLALT
                     PFARAGILRGDVVVDAKSGVSGAGRSAGTDFLYTELEGGTKAYGIPRHRHTPEIAQGL
                     ADAGAEVHLTFVPHLVPQVRGIVATCYLTLADDLSDDDLAGILDAAYADEPFVHRVTK
                     PPSSKLAWGSNHAFVFAARTAERRAVAVAAIDNLGKGAAGQAVQNMNVALGLPETAGL
                     TGIGVYP"
     gene            1824..3254
                     /locus_tag="VFV59_05410"
     CDS             1824..3254
                     /locus_tag="VFV59_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEU5325303.1"
                     /translation="MQTATPAPAKTPVPPHRLELAADTLTPVAAALRLGSRCAFLLES
                     VEEGARYGRYSIVGVHGRTLSVRADIATLTGGDGERTFTAADPLEALGQVLAEQAPTE
                     ADPAFPIGSGVGYLAYESAARWERLPVPDADPLGLPDAVFHLPSAVIVFDHLAQVARV
                     TSTSTNGADVRLEEVARLLGTPSRDEIPARFDPVDVPVDRDPAGRATYEAGVARLVEE
                     IHAGEMLQCVLARRFTVPTRRSAIEVYRALRRINPSPYLFLLDLSPVAAAGTALVGAS
                     PELLVRVRGGEVVTRPIAGTRPRADDPATDAALEDELRADPKELAEHAMLVDLARNDV
                     GRVAAAGSVEVASLRTVERYRHVMHLVSEVHGRLREGLDAFDAVRAVFPAGTLSGAPK
                     VRAMQAIAELEGERRGPYGGAIGYFSPTDVECAITIRSAVVGEGRATVHAGAGVVADS
                     VPEREAAEVAAKAASMLAAIGPEVAS"
     gene            3251..3841
                     /locus_tag="VFV59_05415"
     CDS             3251..3841
                     /locus_tag="VFV59_05415"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007427436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU5325304.1"
                     /translation="MSGPRVFLLDNYDSFTWNLVHLLQAVGATVSVARNDEVTVEEVA
                     AMDPDAIVISPGPSRPERAGISVELVRALGASVPILGVCLGHQAIGVAYGARVIRVPP
                     VHGKAGSVHHDGTGSFAALPSPLSAARYHSLAIDPLGLPDGLEVTARSEDGVVMAVRH
                     RTHPVEGFQFHPESILTDDGAALVDAFLARTTGVPA"
     gene            3838..4878
                     /gene="trpD"
                     /locus_tag="VFV59_05420"
     CDS             3838..4878
                     /gene="trpD"
                     /locus_tag="VFV59_05420"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5325305.1"
                     /translation="MNDVQPTEPMRPAVAAAVEVAAGGGHLPAPLAEAAMEELMDGSA
                     PPVQVAALLAMLRLRGETPEELAAFARVMRSRAVHVDAPEDAVDLCGTGADGLHTFNI
                     STLSAFVVAGAGAPVAKHGNRAITSRSGSADLVEALGIPLDPGPEEVARSIRDTGFGF
                     IFAPSYHPAMKHAMPIRRELPMRTIFNLLGPLSSPARVRRQLLGVADPALLSLVAGAL
                     SRLEARRALVVHGHDGTDELSLGGPNQALLVEDGEVRELVIDAAELGLERAPLATLVG
                     GDAQLNSAIAISVLGGEPGPARDVVLLNAGAALFVAGVAADVAEGVGLAADAIDSGRA
                     ADVVSAAAGAAP"
     gene            4875..6314
                     /locus_tag="VFV59_05425"
                     /pseudo
     CDS             4875..6314
                     /locus_tag="VFV59_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6321..7544
                     /gene="trpB"
                     /locus_tag="VFV59_05430"
     CDS             6321..7544
                     /gene="trpB"
                     /locus_tag="VFV59_05430"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5325306.1"
                     /translation="MSAARTDPTGADRVDTRGRYGPFGGRYVPETLIPPLDELVGAWT
                     AARRDAGYRADLARLQRDFIGRPTALFEVAPTALADTARTDVRVFLKREDLAHTGAHK
                     INNAIGQALLARRMGKTRIVAETGAGQHGVATAAACALLGLTCVVYMGSTDVERQAPN
                     VRRMHLLGAEVRPVTTGHGTLRDALNEALRDWVANVETTAYILGSAAGPHPYPDMVAE
                     LQSVIGREARAQLLERVGRLPDLAVASVGGGSNAIGLFRPFLDARTRLLGVEAGGRGD
                     GLGDNAASLGLGSPGVLHGAFTMLTQTEDGQVVEPHSVSAGLDYPGVGPQLSALTAVG
                     RLEVARTSDAEAVAALHRLTRCAGILPALEPSHALAAVGRLLPSLPADAVVVVNLSGR
                     GDKDLDIVDRFGGAA"
     gene            7541..8359
                     /gene="trpA"
                     /locus_tag="VFV59_05435"
     CDS             7541..8359
                     /gene="trpA"
                     /locus_tag="VFV59_05435"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU5325307.1"
                     /translation="MSLPPGARRIRDAFASAEGDGRRVLVTYLMAGHPSEEDATDAAD
                     AALRAGADILEIGVPFSDPVADGPLIAAAGHAAVAAGAGMATVLRTLRELRRRGREHP
                     IVAMGYLNPMVAHGVERTLAELRDAGLDGLIVPDLPAGEDPRLERAIGRAGLGVTFLV
                     TPNSTEERLERTIRASTAFLYVVPLYGVTGVRQSVAATTGPLLERIRSRVAGRVPVAV
                     GFGVSRPEHVRELATVADGIIVGSAIVAAQNDAGAEGVASLVTDLAAASTLVPA"
     gene            complement(8401..9390)
                     /locus_tag="VFV59_05440"
     CDS             complement(8401..9390)
                     /locus_tag="VFV59_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5325308.1"
                     /translation="MTLLPLSPGEPPLRLGLFRTEGGAIIPDLRVAFRRDGPRSASAV
                     LVIHALTGSADAAGDWWKPLIGAGLALDTGATQVVSLNLLGSCYGTTGPRDLDPFPPI
                     TPRDMARAQWAALDALGVERVELAVGGSLGGMVALEVALERPASVAAVMPIAAPASIG
                     NLAAGWNHIQLQLLDLDRERGLELARQLAMTTYRSEVDFEARERIGSYLEHQGTKLRL
                     RFHAGSYAALVAAMNAHDIGRDRGGVVAALSTLASGGTRLHGVGIEGDLLYGPRQVRA
                     LVDAARLAGVTASYDEIRSDKGHDAFLVEWDQLTGILRRALQGRDEGRYAASA"
     gene            complement(9387..10709)
                     /locus_tag="VFV59_05445"
     CDS             complement(9387..10709)
                     /locus_tag="VFV59_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEU5325309.1"
                     /translation="MSETNGTDTPRTPRTETLALHAGQQPDPTTGSRAVPIYATSSYS
                     FQDADHAARLFALQEFGNIYTRIMNPTTDVFEQRMAALEGGVGALGLASGQAAETLAI
                     LNLAGAGDNIVASTSLYGGTFNLFAYTLPRLGIEVRFVEGNAPDGFVTQTDARTRAYY
                     VETIGNPRLDVPDLAAIANAAHGEGLPLIVDNTFAPLIARPIDHGADIVVHSATKWIG
                     GHGTSIGGVVVDGGRFDWAASPRFPQFVEPDPSYHGVSYTTAFGPAAFILKLRVQLLR
                     DLGPALSPFNSWLFIQGLETLPLRIARHSENALAVARWLEADPRVEWVSYPGLESHPT
                     HANATRYLDGGFGGMLTFGVRGGATAGRRLIDSVKLFSLLANVGDAKSLIIHPASTTH
                     QQLDAEAQRKSGVTPDLVRLSVGIEAIEDIIADLDQALDVATNEELAA"
     gene            complement(11018..>11491)
                     /locus_tag="VFV59_05450"
     CDS             complement(11018..>11491)
                     /locus_tag="VFV59_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018253099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5325310.1"
                     /translation="AFVGDGNNVCHSLLLAGSLAGMHLRVATPPGYEPDPAVVRAALG
                     TARWRGGSVVLGTDPVSAVRGADAVYTDAWTSMGAEAEADLRRLRFVGFRVDEALLAH
                     APGALVMHCLPAHRGEEITDAVLDGPQSIVLDQAENRLYVQQALLVDLLGADPED"
BASE COUNT         1471 a         4161 c         4295 g         1564 t
ORIGIN      
        1 aggccggtcg aggcggtgag gacgaaccga tcggcgacgc ccagccgatc ggcgacgaga
       61 tgctggaccg ctcgggcatc cgccagcccc ggcgtgccgg tgcccgcgtt ggcgcatccc
      121 gcgttcgcca cgatggcggc ggcgtggccc gctgaggagc gcagcgagcg catcgagacc
      181 tcgaccggcg cggcgcgcag ccggttgcgg gtgaacgtcg ccgcggctgg aacgggaccc
      241 cgctcgctga cgaggagcat cacgtccggg cccatcccgg agcggagccc ggcgcgtgcc
      301 gccgccaccc ggaacccgga gggcgccgtg acgccgccgt cgatgcggcg aacggtcacg
      361 gatagacccc gatcccggtg agtcccgcgg tctcgggcag tcccagcgcc acgttcatgt
      421 tctgcacggc ctgtccggcc gcgcccttgc ccaagttgtc gatggccgcg acggccaccg
      481 cccgtcgctc tgcggtgcgg gccgcgaaga cgaacgcgtg gttggagccc caggcgagct
      541 tcgaggacgg gggcttcgtc acgcggtgca cgaacggctc gtcggcgtac gccgcgtcca
      601 ggatccccgc gagatcgtcg tccgagaggt cgtcggcgag ggtcaggtag caggtcgcca
      661 cgatgccgcg cacctgcggc accaggtgcg ggacgaaggt gaggtgcacc tcggcgccgg
      721 catccgccag gccctgcgcg atctcgggcg tgtggcggtg tcgagggatg ccgtacgcct
      781 tcgtgccgcc ctcgagctcg gtgtagagga agtccgtccc ggcgctgcgc ccggcgcccg
      841 agacgccgga cttcgcgtcg accaccacgt cgccgcgcag gatgccggca cgcgcaaagg
      901 gtgtgagcgc cagcagggag gccgtcgggt agcagccggg attcgcgatc agccgcgcgc
      961 cgcggagtcg gtcgcgggcg aactcggtga ggccgtagac cgcatcggcc aggacgtctg
     1021 gagccgggtg atcgaagccg taccagtccg ggtaggcggc gggatcgcgc agccggaggt
     1081 cagagccgat gtcgatcacc gtcgtgcctc cgttggccag gcgcccgctg agctcggcgg
     1141 aggcgccgtg cggcagcgcc aggaaggcca cgtcgatgct ccccggctcc ggggcgccgt
     1201 cctccagtcg cacgccgagt ggggcggtgt gcgggtattc ctccgccatg ggcgtggcgt
     1261 cacggccacg ggcgtacagc gtgtggatcg tgacgtcggg atgtccggcg aggagtcgga
     1321 cgagctcggc gccggcatag ccggatgcac cgacgacggc gactcggacg ggagcgttca
     1381 ccgcagaagt atgcaacttg tctgcataca catgcaatgg tgggtcaacg tgcaggtcct
     1441 tgacgcgcat cggcggcgca cctaggatgc gactccaact tcagacggcg tcgaccggga
     1501 cgagtacctc gtcgcacggc caccgcagcg agccgggatg gtgcaagccg gcgtggcacg
     1561 ggacgtggga atggacccgc gagcctcgct ccgaacgtgg ttcgggccgc ggtggatatc
     1621 gggatatacg ccaggcaccg ggcaccaact aggcggcgac gggaggcccc cgttacctcg
     1681 ggccggcgca tcggcgctcc agatgggagc gacccgtgcg agccgagtgc ggaccatcgg
     1741 tccgaagcag ggtggcaccg cggaagaagc ctccgtccct cgaggatgga ggctttcgtg
     1801 attctcgcga agaagaccaa ccgatgcaga ccgccacccc cgcacccgcc aagacaccgg
     1861 tacccccgca ccgcctggag ctggcggccg atacgctcac gcctgtcgcg gccgcgctcc
     1921 ggctgggcag ccgatgcgcc ttcctgctcg aatcggtcga ggagggcgcc cggtacggtc
     1981 gctactcgat cgtcggggtg cacggccgca cgctgagcgt ccgggccgac atcgcgacgc
     2041 tgaccggtgg tgacggcgag cgcaccttca ccgcggccga tccgctggag gcgctgggcc
     2101 aggtcctggc tgagcaggcc ccgaccgagg cggaccccgc cttcccgatc ggcagtgggg
     2161 tgggctacct ggcgtacgag agcgcggcgc gctgggagcg gctgccggtg cccgatgccg
     2221 atcccctcgg cctgcccgac gcggtcttcc acctgccgtc agcggtgatc gtcttcgacc
     2281 acctggcgca ggtcgcgcgc gtcacgagca cgtcgacgaa cggcgccgat gttcggctgg
     2341 aggaggtcgc gcgactgctc ggcacgccct cgcgcgacga gatccccgcg cggttcgatc
     2401 cggtcgacgt cccggtcgac cgcgatcccg cgggccgggc gacctacgag gccggcgttg
     2461 cgcgactggt cgaggagatc cacgccgggg agatgctcca gtgcgtcctg gcgcgccggt
     2521 tcacggttcc gacgcggcgg tcggcgatcg aggtgtaccg ggcgctgcgc cgcatcaacc
     2581 cctcgccgta cctgttcctg ctggatctct cgccggtagc ggcggcgggg acggcgctgg
     2641 tcggggcgtc gccggagctg ctggtgcgcg tccgcggcgg cgaggtggtc acgcggccga
     2701 tcgccggcac gcgcccgcgt gcggacgacc cggccacgga tgcggcgctg gaggacgagc
     2761 tgcgcgccga tccgaaggag ctggcggagc acgccatgct cgtcgacctg gctcgcaacg
     2821 acgtgggacg ggtggcggcg gcgggcagcg tcgaggtggc ctcgctgcgg accgtggagc
     2881 gctaccgcca cgtgatgcac ctggtctcgg aggtgcacgg ccggctgcgc gagggcctcg
     2941 acgcgttcga cgcggtccgc gccgtcttcc ccgccggcac gctctccggc gcgcccaagg
     3001 tgcgcgccat gcaggccatc gccgagctcg agggggagcg cagaggcccc tacggcggcg
     3061 ccatcggcta cttcagcccg accgatgtcg agtgcgccat caccatccgc tcggccgttg
     3121 tcggcgaggg ccgcgcgacg gtgcatgccg gcgccggggt cgtcgcggac tcggtcccgg
     3181 agcgcgaggc ggccgaggtc gcggccaagg cggcgagcat gctcgccgcc atcggtcccg
     3241 aggtggcgtc gtgagcggcc cgcgcgtctt cctgctcgac aactacgact cgttcacgtg
     3301 gaacctggtt cacctgctcc aggccgtcgg tgcgaccgtc agcgtggcgc gcaacgacga
     3361 agtgacggtc gaggaggtcg cggcgatgga tccggacgcg atcgtcatct cgccgggacc
     3421 ctcccgaccg gagcgcgcgg gcatcagcgt cgagctggtg cgcgcgctcg gcgcatcggt
     3481 ccccatcctc ggcgtgtgcc tgggccacca ggccatcggc gtcgcctacg gggcgcgggt
     3541 gatccgcgtg ccgccggtgc acggcaaggc cgggtcggtg caccacgatg gaaccggctc
     3601 gttcgctgcc ctgccgtcgc cgttgagcgc cgccaggtat cactcgctcg ccatcgaccc
     3661 cctgggcctg cccgacggcc tggaggtcac cgcgcgatcc gaggacgggg tggtcatggc
     3721 cgtgcgccat cggacgcatc cggtggaggg cttccagttc catcccgaat ccatcctgac
     3781 cgatgacggc gccgccctcg tggacgcctt cctcgcccgc accaccggag tccccgcatg
     3841 aacgacgtcc agcccaccga accgatgcgc cccgccgtgg ccgccgccgt cgaggtcgcc
     3901 gccggcggcg gccatctccc cgcgccgctg gccgaggccg ccatggagga gctgatggac
     3961 ggctccgcgc cgcccgtcca ggtcgccgcg ctgctcgcca tgctgcggct gcgcggcgag
     4021 acgccggagg agctggccgc gttcgcgcgc gtgatgcgct cacgggccgt gcacgtggac
     4081 gcgcccgagg acgcggtgga cctgtgcggc accggcgccg acggcctgca caccttcaac
     4141 atctcgacgc tctccgcgtt cgtcgtcgcg ggcgccggtg cgccggtcgc caagcacggc
     4201 aaccgtgcca tcacgtcgcg ctccgggtcg gcggacctgg tcgaggcgct cggcatcccg
     4261 ctcgaccccg ggccggagga ggtcgcgcgc tccatccgcg acaccggctt cggcttcatc
     4321 ttcgcgccct cctaccaccc ggccatgaag cacgccatgc cgatccgccg ggagctgccg
     4381 atgcgcacga tcttcaacct gctcgggccg ctcagctcgc ccgctcgcgt gcggcggcag
     4441 ctgctcggcg tcgcggatcc cgccctgctc tccctggtcg cgggggcgct gagccggctg
     4501 gaggcgcggc gtgcactggt cgtgcatggc cacgacggca cggacgagct gtcgctgggc
     4561 ggtcccaacc aggcgctgct ggtcgaggac ggcgaggtcc gcgagctcgt gatcgatgcg
     4621 gcggagctgg gcctggagcg ggcgccgctg gcgacgctgg tcggcggcga cgcgcagctg
     4681 aactccgcga tcgccatctc ggtgctgggc ggggagccgg ggccggcgcg ggacgtcgtc
     4741 ctgctcaatg ccggcgcggc cctcttcgtg gcgggcgttg ccgcggacgt cgccgagggc
     4801 gtcgggctgg cggcggacgc catcgactcc ggacgcgccg ccgacgtcgt gagcgccgcc
     4861 gccggggccg cgccgtgagc gtcctcgagc cgctgctgtc cgcctcgcgc acgcgagcgc
     4921 gccgcgacgc gcgcaacgag acactgccgg agcttcggcg tcgcgtcgag cgcatgccgc
     4981 cggccccgcg cttcgaggcc gcgctgcggt acgcggaccg cctggcgctc gtggcggagg
     5041 tcaagcgcgc gtcaccgtcg gccggcagct tcgacgccgt tggtgctggt gcggacgctg
     5101 ttcgtgacct ggcccttcgg tacgcggacg ccggcgcggc ggcgctctcg gtgctcaccg
     5161 agccgactcg cttcggcggc agcgatcggg acctggaggc ggccgcgccc atcggtccac
     5221 ccgtgctgcg caaggacttc accgtcacgc cgtaccaggt ctggcaggcg cgcgccctcg
     5281 gggccggtgc ggtgctgctg atcgtccgcg ccctggacga cgtcaccctc cgctcgctgc
     5341 tcgcaacgtc cgcggaggcc ggcgtggacg cgctggtcga ggtccacacc gcggaggagc
     5401 tggaccgcgc cctggcctgt gacgcgaccc tgatcggggt caacgcccgc gacctggaca
     5461 cgctggacgt gtcgctggac cgggcgttcg agctgctcga gtcggcgcgc tcctccgggg
     5521 cgacgctcgt cgccgagagc ggcctggcca cgctgtccga ccttcgcgcg gcggccgatg
     5581 cgggcgccca cgcggtcctc gtcggcaccg cgctcctgca cgcgcccgat cccgcggcgc
     5641 gggtgcgtga gctggtcgac ggagcgccac ggcgcgcacc ggggccggtt cccgcgacgc
     5701 cccggcggtc gatggtcaag gtctgcggat tgcgggacac ggcgggcgtt cgcgccgccg
     5761 tcgccgcgga cgccgatctg gcggggttca tcgtggcgcc gtcacggcga caggtggcgc
     5821 ccgaggaggc gggacgcctg gcgcagggct tgggcaccgt gcagccgatc ctggtattcc
     5881 gctcgccctc cgcgtcggag ctcgacgacg cgatcggcgc cttcggggga taccggacac
     5941 cgggcgtcca gctggcgggc ttcgacgagc ccccgccgtg gcttgcggag cgcgcccccg
     6001 cgctgcccgc ggtgatcggg gtcgtccacc gcccgacgtc ggtgcgttcg gccctgcgcc
     6061 tggcggagcg ctggtacgcc gccggcgcca cgcacctgct gctcgagggc gcccccgcga
     6121 ccgatggcgg cggcaccggc agcgcggcgc ccctcgacgt cgcccggcgg ctcaaccgga
     6181 tcctgccggc cggggtcgct ggtggcctgg acccgaccaa cgtcgcggcg gcggtgcgtc
     6241 acgcgcggcc cgccctggtg gacgccgcat ccggcctcga gcgtgcggga cagagcgacc
     6301 ctcggcgcat cgcccgattc gtgagcgcgg cccggaccga tcccacgggg gccgaccgcg
     6361 tcgacacgcg tggccggtac ggacccttcg gcggccgcta cgtccccgag acgctgatcc
     6421 cgcccctgga cgagctggtg ggtgcgtgga ccgcggcgcg gcgcgacgcc ggatatcggg
     6481 cagacctggc gcgtctccag cgcgatttca tcggccggcc cacggcgctg ttcgaggtcg
     6541 caccgacggc cctggcggac acggcgcgga ccgacgtgcg cgtgttcctg aagcgcgagg
     6601 acctggcgca caccggcgcg cacaagatca acaacgccat cggccaggcg ctcctggcga
     6661 ggcgcatggg caagacccgg atcgtggcgg agaccggggc cggacagcac ggcgtggcga
     6721 cggcggccgc ctgcgcgctg ctggggctca cctgtgtcgt gtacatgggc agcaccgatg
     6781 tcgagcgcca ggcgccgaac gtccgacgca tgcacctgct cggcgcggag gtccggccgg
     6841 tgaccaccgg tcacggcacg ctgcgcgacg cgctgaacga ggcgctgcgg gactgggtgg
     6901 ccaacgtcga gacgacggcg tacatcctcg ggtccgccgc cgggccgcac ccgtacccgg
     6961 acatggtggc cgagctccag tcggtcatcg gccgcgaggc gcgggcccag ctgctggagc
     7021 gggtcgggcg gctgcccgac ctggccgtgg catccgtcgg cggcggaagc aacgccatcg
     7081 gcctcttccg cccgttcctg gacgcccgta cccgcctgct cggcgtggag gccgggggac
     7141 gcggggacgg gttgggcgac aacgccgcct cgctgggcct cggctcaccg ggtgtgctgc
     7201 acggcgcgtt caccatgctg acccagaccg aggacgggca ggtcgtcgag ccccactcgg
     7261 tctcggccgg gctcgactac cccggggtcg ggccgcagct ctcggcgctg acggcggtcg
     7321 gtcgcctgga ggtggcgcgg acgtccgacg cggaggcggt ggccgccctc catcggctca
     7381 cgcggtgcgc cgggatcctt cccgcgctgg agccctcgca cgcgcttgcc gccgtgggcc
     7441 ggctgctgcc gtcgctgccg gccgatgcag tggtcgtggt caacctctcc ggccgcgggg
     7501 acaaggacct ggacatcgtg gatcgattcg gtggtgccgc atgagcctgc cgcccggcgc
     7561 acgccgcatc cgcgacgcct tcgcgtcagc cgaaggggat ggccgtcggg tgctggtcac
     7621 ctacctcatg gccggccacc cgagcgagga ggacgcgacc gacgcggccg atgcggcgct
     7681 gcgcgccggc gccgacatcc tggagatcgg ggtgccgttc tccgaccccg tggccgatgg
     7741 accgctcatc gccgccgcgg gccacgccgc cgtcgcggcg ggggccggca tggccacggt
     7801 cctgcgcacc ctgcgtgagc tgcggcgacg cgggcgggag catccgatcg tggccatggg
     7861 gtatctcaac ccgatggtgg cgcacggcgt cgagcgcacg ctcgccgagc ttcgtgacgc
     7921 cgggctggac ggcctcatcg tgccggacct gccggccggg gaggatccac gtctcgagcg
     7981 cgccatcggc cgggcgggcc tgggcgtcac gttcctcgtc acgccgaact cgaccgagga
     8041 gcggctggag cgcacgatcc gggcctcgac cgcgttcctc tacgtcgtcc cgctgtacgg
     8101 cgtgacgggc gtccgccagt cggtggccgc cacgacgggc ccgctgctgg agcggatccg
     8161 atcacgcgtc gccggccgcg tgccggtggc cgtcggcttc ggcgtctcgc gtcccgagca
     8221 cgtccgcgag ctggcgacgg tggcggacgg gatcatcgtc gggtcggcca tcgttgccgc
     8281 ccagaacgac gccggcgccg agggcgtcgc ctcgctcgtc acggacctgg cggccgcctc
     8341 gaccctggtc cccgcctgac gccggagcct gccttgcgct gaacgtcggc cgctccgcgc
     8401 tcaggcgctg gcggcatagc gcccctcgtc ccgaccctgc agcgcgcgcc gcaggatgcc
     8461 cgtgagctgg tcccactcga ccaggaacgc gtcgtggccc ttgtccgacc ggatctcgtc
     8521 gtagctggcg gtcacgccgg ccaggcgcgc cgcgtcgacc agcgcacgga cctggcgcgg
     8581 gccgtacagc aggtccccct cgattccgac accgtgcagc cgcgtgccgc ccgaggcgag
     8641 cgtcgacagc gccgccacga cgccgccgcg atcgcgaccg atgtcgtgcg cgttcatcgc
     8701 cgccaccagc gccgcgtacg agccggcatg gaaccgcagc cgcagcttgg tcccctggtg
     8761 ctcgaggtag gagccgatgc gctcgcgcgc ctcgaagtcc acctcggagc ggtaggtcgt
     8821 catcgccagc tgccgggcca gctcgagccc gcgttcccgg tcgaggtcga gcagctgcag
     8881 ctggatgtga ttccacccgg ccgccaggtt gccgatgctc gccggcgccg cgatcggcat
     8941 gacggccgcc accgacgccg gccgttccag ggccacctcg agggcgacca tgccgcccag
     9001 cgagccgccc accgcaagct cgacccgctc aacgccgagc gcgtcgagcg cggcccactg
     9061 cgcacgggcc atgtcgcgcg gggtgatcgg cgggaaggga tcgaggtccc gcggcccggt
     9121 cgtgccgtag cacgatccaa gcaggttgag cgagaccacc tgcgtggccc cggtgtcgag
     9181 cgccaggccc gcaccgatga gcggcttcca ccagtcgccg gccgcatcgg ccgagccggt
     9241 gagcgcgtgg atcaccagca ccgccgatgc cgatcgcggc ccgtcgcgcc ggaacgcgac
     9301 gcgcaggtcg gggatgatcg cgccgccctc ggtccgaaac agaccgaggc gcagcggcgg
     9361 ctccccggga ctcaggggga ggagcgtcat gcggccagct cctcgttggt ggccacgtcc
     9421 agcgcctggt cgaggtccgc gatgatgtcc tcgatcgcct cgatgccgac cgacagccgc
     9481 accaggtccg gcgtgacgcc cgacttgcgc tgggcttcgg cgtccagctg ctggtgggtc
     9541 gtggaggccg ggtggatgat cagcgacttc gcgtcgccga cgttggcgag gaggctgaac
     9601 agcttcacgc tgtcgatcag ccgccggccg gcggtcgcgc cgcctcgcac gccgaacgtc
     9661 agcatcccgc cgaagccgcc gtccaggtat cgcgtggcgt tggcgtgggt cgggtgcgac
     9721 tcgagccccg ggtagctgac ccactcgacc cgcgggtccg cctccagcca gcgcgccact
     9781 gcgagcgcgt tctcgctgtg ccgcgcaatt cggagcggca gcgtctcgag accctggatg
     9841 aacagccacg agttgaacgg cgacagcgcc ggtccgaggt cccgcaacag ctgcacgcgg
     9901 agcttcagga tgaaggcggc ggggccgaac gcggtcgtgt agctgacccc gtggtacgag
     9961 gggtccggct ccacgaactg cgggaagcgc ggcgaggcgg cccagtcgaa cctcccgccg
    10021 tcgaccacca cgccgccgat gctcgtcccg tgcccgccga tccacttggt ggccgagtgg
    10081 accacaatgt cggcgccgtg gtcgatgggc cgggcgatga gcggcgcgaa ggtgttgtcg
    10141 acaatcagcg gcagcccttc gccgtgagcc gcgttcgcga tcgccgcgag atccggcacg
    10201 tcaagccgcg ggttgccgat ggtctccacg tagtaggccc tggtgcgcgc atcggtctgc
    10261 gtgacgaagc cgtccggcgc gttgccttcg acgaagcgca cctcgatgcc gaggcgcggc
    10321 agggtgtagg cgaacaggtt gaaggtgccg ccgtacaggc tcgttgaggc aacgatgttg
    10381 tcgccggcgc ccgccaggtt gaggattgcc agcgtttcgg ccgcctgccc ggaggccagg
    10441 cccagcgcgc cgacgcctcc ctcgagcgcc gccatgcgct gctcgaagac gtcggtcgtc
    10501 gggttcatga tccgggtgta gatgttgccg aactcctgca gcgcgaagag gcgggcggcg
    10561 tgatccgcgt cctggaagct gtaggagctg gtggcataga tcgggaccgc ccgcgagccg
    10621 gtcgtcgggt cgggctgctg ccccgcgtgc agggcgagcg tctcggtgcg gggggtgcgt
    10681 ggcgtatcgg tgccgttggt ctcgctcatg gtgcttgcgt ccaatctccc tgggtcgacc
    10741 accgggggga gcgaggggcc acgcgtggtg gtcggttggt gcgacgcggc cccggaaggt
    10801 gggggtgtgc ggctgatccg cgtcgccgaa gggatacgaa caggccgcac cgctctcgtg
    10861 gagtggtgcg gccccgatca ggtcctgagc cggatgtcag tggtcagcgc tcaggcagcg
    10921 tcctccaggc ggcggggccg ccagggcata cacatgcagg tgaagtgggg gacgtggtga
    10981 tcggggtaca tcggtgccga ggacgctacc agcggtgtca atcctccggg tccgcgccca
    11041 gcagatcgac gaggagcgcc tgctggacat agagccgatt ctccgcctgg tccagcacga
    11101 tcgactgcgg gccatccagg accgcgtcgg tgatctcctc gccgcggtgc gccggcaggc
    11161 aatgcatgac gagcgccccc ggcgcatgcg ccagcagagc ctcgtccacc cggaagccga
    11221 cgaagcgcag gcggcgcagg tccgcctccg cctcggcacc catgctggtc caggcgtcgg
    11281 tgtagaccgc gtccgcgccg cgcacggcgg acaccgggtc cgtcccgagg accaccgagc
    11341 caccgcgcca ccgcgccgtc cccagggccg cgcgcacgac agcggggtcg ggctcgtacc
    11401 ccggcggcgt cgcgacgcgc aggtgcatgc cggccagcga ccccgccagc agcagcgagt
    11461 ggcagacgtt gttgccgtcg ccgacgaagg c
//
LOCUS       DASTYP010000117         5383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1575320, whole genome shotgun sequence.
ACCESSION   DASTYP010000117 DASTYP010000000
VERSION     DASTYP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575320"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFV59_05455"
     CDS             <1..463
                     /locus_tag="VFV59_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5325311.1"
                     /translation="DAVRAMAEAAERLVPELGERLRRHGLGEIEVTEGELHVRVVADP
                     AVTPAATPADAAAPAAAAAPAPAAPGRASTPPPVEVASPAVGFFVYADGLGPGQAVGK
                     GDRIGYVEMLGVRHDVHAPRAGVVSHLVAESGEAVEYGQTVVEIEAEAAAR"
     gene            460..1404
                     /gene="fabD"
                     /locus_tag="VFV59_05460"
     CDS             460..1404
                     /gene="fabD"
                     /locus_tag="VFV59_05460"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5325312.1"
                     /translation="MSLALLFSPQGSQSVGMGRALADASPAARAVFAEADAVLGWSVS
                     SVCFDGPPERLDDTRQTQPALLATSVAALRALEERARLAPAFVAGHSVGEYAALVAAD
                     VLDLAAALRLVARRGELMAAVDVPGGMAAVIGLERDAVQAVVDDVARPTQLVVANDNA
                     PGQVVISGELEALNAAEGAMKAAGARRVLRLPVSGPFHSPLMAPVAEQLEAAFEGEAW
                     RDASIPVVSNVTAEPLTDAGRIRALLAEQVRSPVEWVASVRRMVDEGVDRFVECGPGT
                     ALTNMIKRIAPEARIGNVSDPETLDATVAELARVEIGA"
     gene            1404..2762
                     /gene="accC"
                     /locus_tag="VFV59_05465"
     CDS             1404..2762
                     /gene="accC"
                     /locus_tag="VFV59_05465"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5325313.1"
                     /translation="MFGKILIANRGEIAVRILRACRDLGIPAVVAYSEADRDSLAVKL
                     ADEAICIGPAEARRSYLNQPALISAAMITGCDAVHPGYGFLSEDATFAEACVAHDLAF
                     IGPRPEVLERFASKYAVRRMLAANGLPTVPGSQGIVTDLRDALQQAGDAGYPVLLKPS
                     AGGGGRGMRLVRSPREMETSLPLARSEAQAAFGDDSIYFEKWIEESRHVEVQVLVDRH
                     GNGIHLGERDCSVQRRHQKIIEEGPSPAMDDPTRERLRDLAIRAVVAAGYESAGTLEF
                     LLDGDGNFYFIEINCRIQVEHPVTELLTGVDLIAEQIRLAAGERLSLRQEDIEFRGHA
                     IEFRINAEDPSDNFSPQTGEVESLHLPGGPGVRVDTHLYPGYEVPPFYDSLLAKMIVW
                     GEDREQALARSRRALAELEIGGVKTNVPFHRGIIDNEAFVDARVSTNLLDRVGPAAFV
                     AS"
     gene            2800..3309
                     /gene="fabZ"
                     /locus_tag="VFV59_05470"
     CDS             2800..3309
                     /gene="fabZ"
                     /locus_tag="VFV59_05470"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU5325314.1"
                     /translation="MTTETTSETTPDEGVTLPLEAADIMRILPHRYPFLMVDRVIELE
                     PGKRVVGMKQVTANEPQFTGHFPGRPIMPGVLMVEALAQTAGVAVMTLPEYRGKLGLF
                     AGIDECRFKRMVVPGDTLHLEVTVEKLRGMFGRMRGVARVGEEVAVEATLSIIIPRDQ
                     GLGGGQTDA"
     gene            3302..4573
                     /gene="fabF"
                     /locus_tag="VFV59_05475"
     CDS             3302..4573
                     /gene="fabF"
                     /locus_tag="VFV59_05475"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5325315.1"
                     /translation="MRDGERRVVVTGMGAVTPIGNDVPSFWDSLVNGRSGIGPITTFD
                     PSKVAAKIAGEVKDFDAAETMPRKEVRRNDRYVHFAWAATTEALRDAGLPNPIEDEAL
                     AWRTGAIVGSGIGGINTMIRDIQEATLEGIDKVGPFLVTAMIPDMAAGYIAIYANVRG
                     PNYATVSACSSSNHAIGDSLNIIRRGEADVMIAGGAEAGIGEIPVAAFSAMRALSTKR
                     NDEPTKASRPFDAERDGFVMGDGAGILVLEELEHAKARGARIHAEVVGYGATDDASHI
                     TLPAPGGRGAVESMRVAIADAGLTTEDVDYINAHGTSTPPNDRSETAAIKTLFGERAY
                     RIPVSSTKSMTGHLMGAGGGIEAIASIRAIETGIVPPTINQEHPDPECDLDYVPNEAR
                     ELPDGVAVAMSNSFGFGGHNATLVFRRYEDR"
     gene            4570..5058
                     /locus_tag="VFV59_05480"
     CDS             4570..5058
                     /locus_tag="VFV59_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5325316.1"
                     /translation="MSDHEPDRILAAVDALAPAFERAGLDELEVTVGELRVRLARPRA
                     APVPVASAAAATPSAPAAIAPGPTARTDEPPPFDQAAPGRHLVTAPLTGIWYSAPSPG
                     ARPYVTEGAEVTAGQVIGLIEAMKLFNEIKSDQSGTVTRFLVESGTLVKRKQPLIEVD
                     PG"
     gene            5055..>5383
                     /locus_tag="VFV59_05485"
     CDS             5055..>5383
                     /locus_tag="VFV59_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325317.1"
                     /translation="MTATNTDAKEHAMTNALITGWGMYAPSRVMTNDDLTKLVDTSDE
                     WIVSRTGIRERRIAADDETTTTLSVHAARDALAVAGVDAADVDLIVLGTCSPDYPLPA
                     TACLVAR"
BASE COUNT          801 a         1909 c         1971 g          702 t
ORIGIN      
        1 ggacgcggtg cgggccatgg ccgaggcggc cgaacggctc gtgcccgagc tcggtgagag
       61 gctccgtcgc cacggcctgg gcgagatcga ggtgaccgag ggcgagctcc acgtgcgggt
      121 cgtcgccgat cccgcggtca cccccgcggc cacgccggcg gatgccgccg ccccggccgc
      181 ggccgccgcg ccggcaccgg ctgcaccggg ccgcgcgtcg accccgcccc cggtcgaggt
      241 cgcctccccg gcggtcggct tcttcgtcta cgccgacggc ctcggccccg gacaggcggt
      301 cggcaagggc gaccgcatcg gctacgtcga gatgctcggc gtgcgacacg acgtccacgc
      361 cccacgcgcc ggcgtcgtca gccacctggt ggcggagagc ggcgaggcgg tcgagtacgg
      421 acagaccgtg gtcgagatcg aggcggaggc ggccgcgcga tgagcctcgc cctcctcttc
      481 agcccgcagg gctcgcagtc ggtcggcatg gggcgcgccc tcgccgacgc ctcgccggcc
      541 gcgcgcgccg tcttcgcgga ggccgacgcg gtcctggggt ggtccgtctc gtccgtgtgc
      601 ttcgacggtc cgcccgagcg cctcgacgac acgcggcaga cccagccggc gctcctcgcc
      661 acgtcagtcg ccgcgctccg cgcgctggag gagcgagcca ggctggcgcc ggcattcgtg
      721 gccgggcact cggtcggcga gtacgccgcg ctggtcgcgg ccgacgtcct cgacctcgcc
      781 gcggcgctcc ggctcgtcgc gcggcgcggc gagctgatgg ccgccgtcga tgtgcccggc
      841 ggcatggccg cggtgatcgg cctggagcgc gacgcggtgc aggcggtcgt tgacgacgtg
      901 gcccggccga cccagctggt ggtcgccaac gacaacgccc ccgggcaggt cgtcatctcc
      961 ggcgagctcg aggcgctcaa cgcggccgag ggcgccatga aggccgctgg agcgcggcgc
     1021 gtgttgcggc tgcccgtctc cggcccgttc cactcaccgc tcatggcgcc ggtggcggag
     1081 cagctcgagg ccgcgttcga gggcgaggcc tggcgcgatg cctccatccc ggtcgtgagc
     1141 aacgtcacgg ccgagccgct gaccgacgcc ggtcggatcc gggcgctcct ggccgagcag
     1201 gtccgttcgc cggtggagtg ggtcgcatcg gttcgccgca tggtcgacga gggcgtcgac
     1261 cgcttcgtcg agtgcgggcc cgggaccgcg ttgacgaaca tgatcaagcg catcgcgccg
     1321 gaggcgcgca tcggcaacgt cagcgatccc gagaccctgg acgccacggt cgcggagctc
     1381 gcgcgcgtgg agatcggcgc ctgatgttcg gcaagatcct gatcgccaac cggggcgaga
     1441 tcgccgtccg catcctgcgg gcgtgccgcg acctcgggat cccggcggtg gtggcctaca
     1501 gcgaggcgga tcgcgactcg ctggcggtga agctggcgga cgaggcgatc tgtatcggcc
     1561 cggcggaggc ccgacgcagc tacctgaacc agccggcgct catcagcgcc gccatgatca
     1621 ccggctgcga tgccgtgcac cccggctacg ggttcctcag cgaggacgcg acgttcgccg
     1681 aggcgtgcgt ggcgcacgac ctggcgttca tcgggccgcg accggaggtc ctggagcgct
     1741 tcgcgtcgaa gtacgcggtg cgccgcatgc tcgccgccaa cgggctgccg accgtgccgg
     1801 gcagccaggg gatcgtgacc gacctgcggg acgcgctcca gcaggcgggc gacgcgggct
     1861 acccggtgct gctgaagcca tcggccggtg gaggcggtcg cggaatgcgg ctcgtccggt
     1921 cgccgcgcga gatggagacg tcactgcccc tggcccgctc cgaggctcag gccgccttcg
     1981 gggacgacag catctacttc gagaagtgga tcgaggagag ccggcacgtc gaggtgcagg
     2041 tgctggtgga tcggcacggc aacggcatcc atctcggtga gcgcgactgc agcgtccagc
     2101 gacggcatca gaagatcatc gaggaggggc cctcgccggc catggacgac ccgacgcgcg
     2161 agcggctgcg cgacctggcc atccgggccg tggtcgccgc cggttatgag agcgccggga
     2221 ccctcgagtt cctgctcgac ggcgacggga acttctactt catcgagatc aactgccgaa
     2281 tccaggtcga gcatccggtg accgagctgc tgaccggcgt cgacctgatc gccgagcaga
     2341 tccgtctggc cgccggcgag cgactctcgc tgcggcagga ggacatcgag ttccggggcc
     2401 acgccatcga gttccgcatc aacgccgagg atccgagcga caacttctcg ccgcagaccg
     2461 gtgaggtcga gtcgctccac ctgcccggcg gacccggcgt gcgcgtcgac acgcacctgt
     2521 accccggcta cgaggtcccg ccgttctacg acagcctgct ggccaagatg atcgtgtggg
     2581 gcgaggatcg cgagcaggcg ctggcgcgat cgcgtcgcgc gctggccgag ctcgagatcg
     2641 gcggcgtgaa gacgaacgtg ccgttccacc ggggcatcat cgacaacgag gcgttcgtcg
     2701 acgcccgcgt gagcacgaac ctgcttgacc gggtcggtcc ggccgccttc gtggccagct
     2761 gagccgatcc cgcccatgac cccaccgacg aaaggcgaga tgacgaccga gacgacatcc
     2821 gagaccaccc cggacgaggg cgtgaccctg ccgctcgaag ccgccgacat catgcggatc
     2881 cttccgcacc gctacccgtt cctgatggtc gatcgggtga tcgagctgga gcccgggaag
     2941 cgcgtggtgg gcatgaagca ggtcaccgcc aatgagccgc agttcaccgg gcactttccc
     3001 ggacggccga tcatgcccgg cgtcctcatg gtcgaggcgc tcgcgcagac cgccggcgtc
     3061 gcggtgatga ctctgccgga gtaccgcggc aagctcgggc tgttcgccgg catcgacgag
     3121 tgccgcttca agcggatggt ggtcccgggg gacacgctcc atctcgaggt caccgtcgag
     3181 aagctgcgcg gcatgttcgg ccgcatgcgc ggcgtcgccc gggtcggcga ggaggtggcg
     3241 gtcgaggcga cgctgtcgat catcatcccg cgcgaccagg gactgggtgg agggcagacc
     3301 gatgcgtgat ggggagcgtc gcgtcgtcgt caccggcatg ggcgccgtga cgccgatcgg
     3361 caacgatgtg ccgtccttct gggactcgct ggtcaacggg cggagcggca tcggcccgat
     3421 cacgaccttc gacccgtcga aggtcgcggc gaagatcgcg ggggaggtga aggacttcga
     3481 cgcggcggag accatgccgc gcaaggaggt ccgccgcaac gaccggtacg tccacttcgc
     3541 gtgggccgcg acgacggagg cgctgcgcga cgcggggctg ccgaacccca tcgaggacga
     3601 ggccctggcc tggcggaccg gcgcgatcgt cggctcgggc atcggcggca tcaacaccat
     3661 gatccgcgac atccaggagg cgaccctcga gggcatcgac aaggtcggtc cgttcctggt
     3721 caccgccatg atcccggaca tggcggccgg ctacatcgcc atctacgcca acgtccgggg
     3781 gccgaactac gccaccgtca gcgcgtgctc gagctcgaac catgccatcg gcgactcgct
     3841 gaacatcatc cgccgcggtg aggccgatgt gatgatcgcc ggcggggcgg aagcggggat
     3901 cggcgagatc ccggtcgcgg cgttcagcgc catgcgggcg ctgtcgacga agcgcaacga
     3961 cgagccgacg aaggcgtccc ggccgttcga cgccgagcgc gatggcttcg tgatgggcga
     4021 cggcgcgggg atcctggtcc tggaggagct ggagcacgcc aaggcgcgcg gggcccgcat
     4081 ccacgccgag gtggtcggct acggcgccac cgacgacgcc tcgcacatca ccctgccggc
     4141 acccggcggc cgcggcgccg tggagtcgat gcgcgtcgcg atcgccgacg ccggcctcac
     4201 cacggaggac gtcgactaca tcaacgcgca cggcaccagc acgccgccga acgaccgatc
     4261 ggagaccgcg gccatcaaga cgctcttcgg cgagcgcgcc taccgcatcc cggtctcgag
     4321 cacgaagtcg atgaccgggc acctgatggg cgccgggggc gggatcgagg cgatcgcgtc
     4381 catccgcgcc atcgagacgg gcatcgtgcc gcccaccatc aaccaggagc accccgaccc
     4441 ggagtgcgac ctcgactacg tgccgaacga ggcgcgcgag ctgcccgacg gcgtcgcggt
     4501 ggccatgagc aacagcttcg gcttcggcgg gcacaacgcg acgctggtct tccggcgata
     4561 cgaggaccga tgagcgacca cgagcccgat cgcatcctgg cggccgtcga cgccctggcg
     4621 ccggcgttcg agcgcgccgg cctcgacgag ctggaggtga cggtggggga gctgcgcgtc
     4681 cggcttgcca ggccgcgcgc cgcgcccgtg cccgtggcct cggcggccgc cgcgaccccg
     4741 tccgcaccgg cggcgatcgc gccgggcccg acggcgcgca cggacgagcc gccgccgttc
     4801 gaccaggcgg cgccggggcg gcacctggtg accgcccccc tgaccgggat ctggtactcg
     4861 gcgccctcgc cgggcgcacg gccgtacgtc accgagggcg cggaggtgac ggcgggccag
     4921 gtcatcggcc tcatcgaggc catgaagctg ttcaacgaga tcaagtccga ccaatccggc
     4981 acggtgaccc ggttcctcgt cgagagcggg accctggtca agcgcaagca gccgctgatc
     5041 gaggtcgacc ccggatgacc gcgaccaaca ccgacgcgaa ggagcacgcc atgacgaacg
     5101 ccctgatcac cggctggggc atgtatgcgc cgtcgcgggt gatgaccaac gacgacctga
     5161 cgaagctcgt cgacacgagc gacgagtgga tcgtgagccg gacgggcatc cgcgagcgtc
     5221 gcatcgcggc cgacgacgag acgacgacga cgctgagtgt gcacgccgcg cgcgacgccc
     5281 tggcggtcgc cggcgtggac gccgcggacg tcgacctgat cgtgctcggc acgtgctcgc
     5341 cggactatcc gctgcccgcg accgcctgcc tggtggcccg cga
//
LOCUS       DASTYP010000118         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1611383, whole genome shotgun sequence.
ACCESSION   DASTYP010000118 DASTYP010000000
VERSION     DASTYP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611383"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VFV59_05490"
     CDS             <1..145
                     /locus_tag="VFV59_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HEU5325318.1"
                     /translation="AGQMIEDVRLAVEGRCRIGFHGRTGGMVPTPAEVLTALRAQREE
                     AAS"
     gene            142..909
                     /locus_tag="VFV59_05495"
     CDS             142..909
                     /locus_tag="VFV59_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5325319.1"
                     /translation="MTITAPAPRVIYERPAPLVDRSSHYCPGCGHGIIHRVLAEVMDE
                     LQLAPRTIAVASVGCSVFAYDYLAVDWVEAPHGRAPAVATGVRRVRPDAFVLTYQGDG
                     DLAAIGTAEIVHAAARGERITVIFVNNGIYGMTGGQMAPTTLLGQRTTSSPTGRDPVL
                     AGFPIRITEMLALLPGVAYAARGSVSDPSHIGKAKAMLRTACQAQLDDAGFSIVELLS
                     NCPVGWGMTAPESMAHLAEVEETYPLGVLVDRTKEAR"
     gene            909..1481
                     /locus_tag="VFV59_05500"
     CDS             909..1481
                     /locus_tag="VFV59_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5325320.1"
                     /translation="MERSVVFAGFGGQGLLFAGQVLARAAIRDGLEVFWIPSYGPEMR
                     GGTASCTVILADGQIGSPVVDRYDAAVVMNPPSLGKFAPRVARNGLLLVNTSLVGLDD
                     VPPMPDGATVLPLPCTELASRGGDDKLVSVVALGALVERTAWVTPESVVATIRAVVGE
                     KRPQIVEADIGAFEAGRAAAAREAIAAGGG"
     gene            1532..3163
                     /locus_tag="VFV59_05505"
     CDS             1532..3163
                     /locus_tag="VFV59_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5325321.1"
                     /translation="MPSFTALVDSFLAQEFEDSPVRASSLGLTEYDDRLDDLSEASIL
                     RRNQRDGHWLDEFRAVDAGTLSFDEQIDRDLVISILRGRQIVEDFEVWRRQPDTYLNP
                     GMTGIFTLFLHQLRPMPELVESAVSRMRQIPANLEAGRANLRPELVPAIFLDRAANQA
                     RAGARYVSEILPAQVEDQRLRGQLADAGREAASAYEAYVAFLEEMRPAASREWALGEE
                     RYSALLREKELLGFGARELRDRGRAEYESLAADLTRCARELRGTDDWKAVLDELNEDH
                     PRTPEQMRIGYEEWTERARQYLKEHRLVSFPAGEECAVVPSPPFQRPVLAVASYMSPP
                     PFSDKMLGHFFVPFPPDGASEDEIQKRLASNSYPGIPTTAVHEAYPGHHWHLVMSKAN
                     PSRVRQVFRTPYFSEGWALYAERMMREQGFFTDLRHEMNQYEATIFRAARIIVDTSLH
                     MGEMTWDEAVEFMTTRTGLTEPTAKAEVTRYCAWPTQASSYLTGCLEIVRIREAFFAA
                     HGSSDTDALRAFHDGITSSGGLPIALAERAVLAVA"
BASE COUNT          461 a         1128 c         1153 g          467 t
ORIGIN      
        1 ggcgggccag atgatcgagg acgtccggtt ggccgtggag ggacgctgcc gcatcggctt
       61 ccatggacgc accgggggga tggtgccgac gcccgcggag gtgctgacgg cgctccgggc
      121 acagcgagag gaggccgcgt catgacgatc accgctcccg ccccgcgcgt gatctacgag
      181 cggccggctc cgctggtgga tcggtcgagc cactactgcc cgggctgcgg gcacggcatc
      241 atccaccgcg tcctggcgga ggtgatggac gagctgcagc tggcaccgcg gacgatcgcg
      301 gtcgcgtcgg tcggctgctc ggtcttcgcg tacgactacc tcgccgtcga ctgggtggaa
      361 gcgccacatg gccgcgcccc ggccgtcgcc accggcgtcc gtcgcgtgcg ccccgacgcg
      421 ttcgtgctga cctaccaggg cgacggcgac ctggccgcca tcggcacggc ggaaatcgtc
      481 cacgccgcgg cccgaggcga gcgcatcacc gtcatcttcg tcaacaacgg gatctacggc
      541 atgacgggcg gccagatggc gccgacgacg ctgctcggcc agcgcaccac gtccagcccg
      601 accggccggg acccggtcct cgccggattc ccgatccgca tcaccgagat gctggcgctg
      661 ctgccgggcg tcgcgtacgc ggcgcgggga tcggtctccg atccgtcgca catcggcaag
      721 gccaaggcga tgctgcggac cgcctgccag gcgcagctcg acgatgcggg cttctccatc
      781 gtggagctgc tgtcgaactg cccggtcgga tgggggatga ccgcaccgga gtccatggcc
      841 cacctggccg aggtcgagga gacctatccg ctcggcgtcc tggtcgaccg cacgaaggag
      901 gcgcgctgat ggagcgctcg gtcgtcttcg ccggcttcgg cggccagggg ctgctcttcg
      961 ccggccaggt cctcgcccgc gccgccatcc gcgacgggct cgaggtcttc tggatcccgt
     1021 cgtatggccc ggagatgcgc ggcggtaccg cctcgtgcac ggtcatcctc gcggatgggc
     1081 agatcggctc accggtggtg gaccgatacg acgccgccgt ggtgatgaac ccgccgtcgc
     1141 tcggcaagtt cgcgccgcgc gtcgcgcgga acggcctgtt gctggtcaac acctcgctcg
     1201 tcggcctgga cgacgttccg cccatgcccg acggtgcgac cgtcctgccg ctgccgtgca
     1261 ccgagctcgc gtcacgcggc ggcgacgaca agctggtcag cgtggtggcg ctgggcgcgc
     1321 tggtggagcg aaccgcctgg gtgacgccgg agtccgtggt cgccacgatc cgcgccgtcg
     1381 tgggcgagaa gcggccgcag atcgtggagg ccgacatcgg cgccttcgag gcgggccgcg
     1441 cagcggcggc gcgcgaggcg atcgcggccg gcggcggatg acggcatcgg cctagactgg
     1501 acggcccggg caccaacaca ggagtctccg catgccgtcg ttcaccgcgc tcgtcgattc
     1561 gttcctggcc caggagttcg aggactcgcc cgtgcgggcc agctccctgg gcctcaccga
     1621 gtacgacgac cgcctggacg acctctcgga agcgtcgatc ctcagacgga accagcgaga
     1681 cggccactgg ctcgacgagt tccgagcggt cgacgccggg acgctgtcgt tcgacgagca
     1741 gatcgaccgc gacctggtga tcagcatcct gcgtggtcgc cagatcgtgg aggacttcga
     1801 ggtctggcgc cggcagccgg acacgtacct caatccgggg atgaccggca tcttcaccct
     1861 cttcctgcac cagctgcggc cgatgccgga gctggtcgag tcggcggtca gccgcatgcg
     1921 ccagattccg gcgaacctgg aggcggggag ggcgaacctg cggccggagc tcgttccggc
     1981 catcttcctg gaccgtgccg ccaaccaggc ccgggcgggg gcccgctacg tcagcgagat
     2041 cctccccgcg caggtcgagg accagcggct gcgcggccag ctggccgatg cgggccggga
     2101 ggcggcatcg gcctacgagg cgtacgtcgc cttcctggag gagatgcgcc cggcggcgag
     2161 ccgggagtgg gcgctcgggg aggagcgcta ctcggccctg ctgcgcgaga aggagctgct
     2221 cggtttcggc gcgcgagagc tgcgggatcg cggacgcgcg gagtacgagt cgctggccgc
     2281 cgatctgacc cgatgcgccc gcgagctgcg cggcaccgac gactggaagg cggtcctcga
     2341 cgagctcaac gaggaccacc cgcgcacgcc ggaacagatg cgcatcggct acgaggaatg
     2401 gacggagcgc gctcggcagt acctgaagga gcaccggctc gtctccttcc cggccgggga
     2461 ggagtgtgcc gtcgtgccgt cgccaccctt ccagcggccg gtgctggccg tcgcgtcgta
     2521 catgtcaccg ccgccgttca gcgacaagat gctgggccac ttcttcgtgc cattcccgcc
     2581 ggacggcgcg tcggaggacg agatccagaa gcgcctggcc tcgaactcgt accccggcat
     2641 cccgaccacc gccgtccacg aggcgtaccc ggggcaccac tggcacctgg tgatgtcgaa
     2701 ggccaacccg tcgcgcgtgc ggcaggtgtt ccgcaccccc tacttcagcg agggctgggc
     2761 cctctacgct gagcggatga tgcgggagca gggcttcttc accgacctgc gccacgagat
     2821 gaaccagtac gaggccacga tcttccgggc cgcgcggatc atcgtcgaca cctcgctcca
     2881 catgggcgag atgacctggg acgaggcagt cgagttcatg accacgcgca ccgggttgac
     2941 ggagccgacg gccaaggccg aggtcacgcg gtactgcgcc tggccgacgc aggcgtcgtc
     3001 gtacctcacc ggctgcctgg agatcgtccg catccgggag gccttctttg cggcccacgg
     3061 ctcctccgac accgatgcgc tgcgcgcctt ccacgacggc atcaccagca gcggcgggct
     3121 gccgatcgcg ctggcggagc gggcggtgct ggccgtcgcc tgaccgccga ggtgctgtgc
     3181 gccgcgtgag tcgccattcg atggccgtt
//
LOCUS       DASTYP010000119         7188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1029043, whole genome shotgun sequence.
ACCESSION   DASTYP010000119 DASTYP010000000
VERSION     DASTYP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029043"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..665
                     /gene="rpsL"
                     /locus_tag="VFV59_05510"
     CDS             246..665
                     /gene="rpsL"
                     /locus_tag="VFV59_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU5325322.1"
                     /translation="MPTISQLVRKGRKPATKKVKAPALRRTLNTLKQTTSEGRGAPQR
                     RGVCLQVMTRTPKKPNSALRKVARVRLTNGMEVTAYIPGIGHNLQEHSMVLVRGGRVK
                     DLPAVKYHIVRGTLDAAGVRDRKQGRSKYGAKSPGKS"
     gene            670..1143
                     /gene="rpsG"
                     /locus_tag="VFV59_05515"
     CDS             670..1143
                     /gene="rpsG"
                     /locus_tag="VFV59_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU5325323.1"
                     /translation="MPRRPTIARKTKYAEQLTGTALTLDQFTNKLMHGGKRQLAHRIL
                     EEALARCERQAGRSGVEVMEAALRNAMPLIEVKPKRVGGSTYQIPVEVRADRRVSLGM
                     RWLIDSARKRNGRSMAEKLAAEFMDASNGIGAAVKRREDTHRMAEANRAFSHFKW"
     gene            1148..3256
                     /gene="fusA"
                     /locus_tag="VFV59_05520"
     CDS             1148..3256
                     /gene="fusA"
                     /locus_tag="VFV59_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5325324.1"
                     /translation="MTTSTATRTDRAGTNIPLQRTRNIGIIAHIDAGKTTVSERILFY
                     TKKIYKLGEVHEGAATMDWMEQEQERGITITAAATTCFWNDHRINLIDTPGHVDFTVE
                     VERSLRVLDGAVVVFDGVAGVEPQSETVWRQADKYQVPRFCFVNKLDRTGADFWRVVD
                     MIKDRLGVNAVPLQVPIGAEDQFRGVVDLINMKAITYGNDLGDQIQVGDIPADVQALA
                     DEWREKMIESLADLDDDIAHKYLEGEEFTADELRRALRAGTLAYQIVPVLTGSALKNK
                     GVQPMLDAVIDFLPSPLDVPPVHGRDPRSGDELTRETDADAPFAALAFKIAADPFVGK
                     LAFFRVYSGTLKTGSYVYNSTKGTKERVGRLLQLHANHREEIDQVSAGDIAAAVGLKN
                     TTTGDTLCDESKPIVLESITFPEPVIELAVEPKTKADQDKMAVALQRLAEEDPTFRVS
                     TDQESSQTRIAGMGELHLEVIVDRMLREFKVQANVGRPQVSYREAIRKPVRAEGRFVR
                     QTGGKGQYGHAILQLEPLERGTGFEWESKIVGGTVPREYWRAIEEGVKEAFQGGVMAG
                     YPVIDVKATLVDGSFHEVDSSEMAFKIAGSMAAKDGVGKADPAILEPVMKVEVTTPEE
                     FMGDVIGNLNSKRGQIDGLDERGSSRVVRAHVPLAEMFGYATELRSMTQGRAAYSMEF
                     SRYAEVPTSLANELIAKSKS"
     gene            3289..4491
                     /gene="tuf"
                     /locus_tag="VFV59_05525"
     CDS             3289..4491
                     /gene="tuf"
                     /locus_tag="VFV59_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005385492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5325325.1"
                     /translation="MAKQKFDRSKPHVNVGTIGHVDHGKTTLTAAITKFLSLSGGADF
                     RAFDQIDNAPEERQRGITIAISHVEYQTANRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVEVPSLVVYLNKVDMVDDEMLVDLVEMEVRELLSK
                     YEFPGDDIPVIRGSALKALESTSDDPESPDYASIKELMDAVDSYIPTPERDIDKPFLM
                     PIEDVFGIKGRGTVATGRIERGIVNTGDTVELVGIHAKSRQLVVTGVEMFKKTLDQGQ
                     AGDNVGLLLRGIEREELERGQVLAKVGSVKPGTKFKAEVYVLSKEEGGRHTPFYAGYR
                     PQFYLRTTDVTGAIILPAGVEMIMPGDNVNIDVELITPIAIEQGQRFAIREGGHTVGA
                     GVITEVVG"
     gene            4497..4805
                     /gene="rpsJ"
                     /locus_tag="VFV59_05530"
     CDS             4497..4805
                     /gene="rpsJ"
                     /locus_tag="VFV59_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU5325326.1"
                     /translation="MAKQRIRIRLKAYDHKLLDQSAEQIVETAQRTGADVAGPVPLPT
                     RIEKYTVIRGPFVHKDGREQFEIRTHKRLIDIIDPSTKTVDALMRLSLAAGVDIEIKM
                     "
     gene            4873..5496
                     /gene="rplC"
                     /locus_tag="VFV59_05535"
     CDS             4873..5496
                     /gene="rplC"
                     /locus_tag="VFV59_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU5325327.1"
                     /translation="MPAGLIGRKLGMTRIFGDDGSVVPVSVIEATPNTITRIRDAAKD
                     GYTALQLGAGTARRATKPVAGQFKHLDKEQQRPRNVREVRVDSADGYEVGQQLGVDLF
                     EAGELVDVTGVSKGHGFTGVIARHNFRRGPKSHGSDHHRAPGSVGAGTYPGRVWKGTR
                     MAGHMGNEQVTVKKLKVVKIDPERNLILVKGAVPGARNGLLLVQKAK"
     gene            5499..6122
                     /gene="rplD"
                     /locus_tag="VFV59_05540"
     CDS             5499..6122
                     /gene="rplD"
                     /locus_tag="VFV59_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012369010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU5325328.1"
                     /translation="MPQTTILSPDGAKAGTVDLAESLFAAPINESVIHQAVVRQLAGR
                     RAGTSDTQTRGEVQGGGAKPWRQKGTGRARQGSRRAPHWTGGGVVFGPHPRSYEQKMP
                     TKMRRSALRGVLSAKAADGALRVVEGFGLDDIKTKTMLERLAGWNAEGKVLLVLPARD
                     ERVELSARNLRQVRVVLADSLNVVDLLEADTIVFTSDALSRAQEVYA"
     gene            6119..6415
                     /gene="rplW"
                     /locus_tag="VFV59_05545"
     CDS             6119..6415
                     /gene="rplW"
                     /locus_tag="VFV59_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5325329.1"
                     /translation="MTAQSLHDVLLRPVISEKSTLETERNNYTFAVARDANKFLIKAA
                     VEAEFKVTVLDVRIVNVRPKQKRRGRRTLGTVPGWKKAVVTIAAGDKIELFEAV"
     gene            6418..>7188
                     /gene="rplB"
                     /locus_tag="VFV59_05550"
     CDS             6418..>7188
                     /gene="rplB"
                     /locus_tag="VFV59_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5325330.1"
                     /translation="MPLRSYRPTSPGLRQMTRSTFEEITTDTPHKPLTERLVRRAGRN
                     SQGKLTVRHQGSGHKRLYRVIDWKRDKFGVPARVATIEYDPNRSARIGLLHYADGDKR
                     YMLLPHGIKVGDTVASGPDVEARVGNALPLSRIPLGTQVHNVELSRGRGGQIVRSAGS
                     SAQLLAKEGEFATLRLPSGEVRRVRVECMATIGQVSNLDHENQKIGKAGRSRHMGKRP
                     TVRGSAMNPNDHPHGGGEGKQPTGMPPKTKWGKPAMGLR"
BASE COUNT         1316 a         2516 c         2422 g          934 t
ORIGIN      
        1 agcgccggac gggtggccct cgggtgctcc ctcgcgcagg accttcggtt gacacgtcga
       61 acgggtgttc cgtatactcc gccttcggtg tcccgcctgc gggacgcctt cgtgtgtgcc
      121 cagtcgggca cccttcgaga agtcccatga tgcagccccg agcgggtttc cgtgggcctc
      181 gtcggggtcg aaacaggcga ggcacgcacg gattccacac gcgagtcagg aacgcgaggt
      241 tctcgttgcc aacgatcagc cagctggtgc ggaagggccg caagccggcc accaagaagg
      301 tcaaggcgcc ggcgctgcgc cgcacgctga acaccctcaa gcagaccacc agcgagggac
      361 ggggcgcgcc gcagcgacgc ggcgtctgcc tgcaggtgat gacgcgcacc ccgaagaagc
      421 cgaactcggc gcttcggaag gtcgcccgcg tccgcctgac caacgggatg gaggtcaccg
      481 cctacatccc gggcatcggg cacaacctcc aggagcactc catggtgctc gtgcgcggcg
      541 gccgcgtgaa ggacctgccg gcggtcaagt accacatcgt gcgcggcacc ctcgacgcgg
      601 ccggcgtccg cgatcgcaag cagggccgca gcaagtacgg cgcgaagtcg ccgggcaaga
      661 gctagaccga tgccacgacg tcccaccatc gcgcgcaaga ccaagtacgc cgagcagctg
      721 accggcacgg cgctgacgct ggatcagttc acgaacaagc tgatgcacgg cggcaagcgg
      781 cagctcgcgc accgcatcct cgaggaggcc ctcgcccgct gcgagcgcca ggccggccgc
      841 tccggcgtcg aggtgatgga ggctgcgctc cgcaacgcca tgcccctgat cgaggtcaag
      901 cccaagcggg tcggcggctc cacctaccag atcccggtcg aggtccgcgc cgaccgtcgg
      961 gtcagcctcg gcatgcgctg gctgatcgac tcggcgcgca agcgcaacgg gcgcagcatg
     1021 gccgagaagc tggcggccga gttcatggat gccagcaacg gcatcggcgc ggcggtcaag
     1081 cgccgcgagg acacgcaccg catggcggag gcgaaccgcg ccttcagcca cttcaagtgg
     1141 taggccgatg accaccagca ccgccacccg caccgaccgc gcgggcacga acatccccct
     1201 ccagcgcacc cgcaacatcg ggatcatcgc ccacatcgac gcgggcaaga ccacggtgtc
     1261 cgagcggatc ctcttctaca ccaagaagat ctacaagctc ggcgaggtcc acgagggcgc
     1321 tgccaccatg gactggatgg agcaggagca ggagcggggg atcaccatca ccgccgcggc
     1381 caccacctgc ttctggaacg accatcgcat caacttgatc gacacgcccg ggcacgtgga
     1441 ctttacggtc gaggtcgagc gcagcctgcg cgtgctcgac ggcgcggtcg tcgtcttcga
     1501 cggcgtggcg ggcgtggagc cgcagtccga gaccgtgtgg cgccaggccg acaagtacca
     1561 ggtgccgcgc ttctgcttcg tcaacaagct cgaccgcacc ggggccgact tctggcgcgt
     1621 ggtcgacatg atcaaggacc gcctgggcgt caacgccgtg ccgctgcagg tgccgatcgg
     1681 cgccgaggac caattccgcg gcgtggtcga cctgatcaac atgaaggcga tcacctacgg
     1741 caacgacctg ggcgaccaga tccaggtcgg cgacatcccg gccgatgttc aggcgctggc
     1801 cgacgagtgg cgcgagaaga tgatcgagtc gctggccgac ctcgacgacg acatcgcgca
     1861 caagtacctc gagggcgagg agttcacggc cgacgagctg cgtcgcgcgc tgcgcgccgg
     1921 cacgctggcc taccagatcg tccccgtcct caccggctcg gcgctgaaga acaagggcgt
     1981 ccagccgatg ctcgacgccg tcatcgactt cctgcccagt ccgctcgacg tgccgccggt
     2041 ccacggccgt gacccgcgct ccggcgacga gctgacgcgc gagaccgatg ccgatgcgcc
     2101 gttcgcggcc ctcgccttca agatcgccgc cgaccccttc gtcgggaagc tggccttctt
     2161 ccgcgtctac tccggcacgc tcaagaccgg ctcgtacgtg tacaacagca cgaagggcac
     2221 gaaggagcgc gtgggccggc tgctgcagct gcacgccaac caccgcgagg agatcgacca
     2281 ggtcagcgcc ggcgacatcg ccgccgcggt cggcctgaag aacacgacga ccggcgacac
     2341 gctgtgcgac gagtccaagc cgatcgtgct ggaatcgatc accttccccg agccggtcat
     2401 cgagctcgcg gtcgagccga agaccaaggc ggaccaggac aagatggcgg tcgcgcttca
     2461 gcgcctggcc gaggaggatc ccaccttccg cgtcagcacc gaccaggaga gctcgcagac
     2521 ccgcatcgcc gggatgggcg agctgcacct cgaggtgatc gtcgaccgca tgctgcgcga
     2581 gttcaaggtg caggccaacg tcggccgccc gcaggtgagc taccgggagg cgatccgcaa
     2641 gcccgtccgc gccgaggggc gcttcgtgcg ccagaccggc ggcaagggcc agtacggcca
     2701 cgccatcctg cagctggagc cgctcgagcg cggcaccggg ttcgagtggg agtcgaagat
     2761 cgtcggcggc accgtgccgc gcgagtactg gcgggccatc gaggagggcg tcaaggaggc
     2821 cttccagggc ggcgtgatgg ccggctaccc ggtcatcgac gtcaaggcca cgctggtcga
     2881 cggctcgttc cacgaggtcg actcgtccga gatggcgttc aagatcgccg gctcgatggc
     2941 cgccaaggac ggcgtcggca aggccgatcc ggccatcctc gagccggtca tgaaggtcga
     3001 ggtgacgacg cccgaggagt tcatgggcga cgtcatcggc aacctcaaca gcaagcgtgg
     3061 ccagatcgac ggtctcgacg agcgcgggtc cagccgcgtc gtgcgagctc acgtccccct
     3121 ggccgagatg ttcggctacg ccaccgagct gcgcagcatg acccagggtc gcgccgccta
     3181 ctcgatggag ttcagccgct atgcagaagt tcccaccagc ctcgccaacg agctgatcgc
     3241 gaagtccaag agctagtacg gaccgataag gagcgacgaa gaccaaccat ggccaagcag
     3301 aagttcgacc gcagcaagcc gcacgtgaac gtcggcacca tcggtcatgt cgaccatggc
     3361 aagacgacgc tgacggctgc catcaccaag ttcctgtcgc tgtccggcgg cgcggacttc
     3421 cgtgcgttcg accagatcga caatgctccc gaggagcgcc agcgcggcat caccatcgcc
     3481 atcagccacg tcgagtacca gacggcgaac cgccactacg cgcacgtcga ctgccccggc
     3541 cacgccgact acatcaagaa catgatcacc ggtgccgccc agatggacgg cgcgatcctg
     3601 gtcgtgtccg ctgccgacgg tccgatgccc cagacccgcg agcacatcct cctcgcccgc
     3661 caggtcgagg tcccgtcgct ggtcgtctac ctgaacaagg tggacatggt cgacgacgag
     3721 atgctggtcg acctggtcga gatggaggtc cgcgagctcc tcagcaagta cgagttcccg
     3781 ggcgacgaca tccccgtcat ccgcggctcg gcgctgaagg cactcgagtc gaccagcgac
     3841 gatccggaga gcccggacta cgcctccatc aaggagctga tggacgcggt cgacagctac
     3901 atcccgaccc ccgagcgcga catcgacaag ccgttcctga tgccgatcga ggacgtcttc
     3961 ggcatcaagg gtcgtggcac ggtcgcgacc ggccgcatcg agcgcggcat cgtcaacacc
     4021 ggcgacaccg tcgagctggt cggcatccac gccaagagcc gccagctggt ggtcaccggc
     4081 gtcgagatgt tcaagaagac gctcgaccag ggccaggccg gcgacaacgt cgggctcctg
     4141 ctgcgcggca tcgagcgcga agagctcgag cgcggccagg tgctcgccaa ggtcggcagc
     4201 gtgaagcccg gcaccaagtt caaggccgag gtgtacgtcc tctcgaagga agagggtggc
     4261 cgccacacgc cgttctacgc cgggtaccgg ccgcagttct acctgcggac caccgacgtg
     4321 accggcgcga tcatcctgcc cgcgggcgtc gagatgatca tgcccggtga caacgtcaac
     4381 atcgacgtcg agctgatcac cccgatcgcg atcgaacagg gtcagcgctt tgcgattcgc
     4441 gagggcgggc acaccgtcgg cgcgggcgtc atcaccgagg tcgtcggcta gccatcatgg
     4501 ccaagcagcg catccgcatc cggctgaagg cgtacgacca caagctgctc gaccagtcgg
     4561 ccgagcagat cgtggagacc gcgcagcgga ccggcgccga cgtcgccggc ccggtgccgc
     4621 tgccgacccg gatcgagaag tacacggtca tccggggccc gttcgtccac aaggacggcc
     4681 gggagcagtt cgagatccgg acgcacaagc gcctcatcga catcatcgat ccgtccacca
     4741 agacggtcga tgcgctgatg cggctgtcgc tcgcggccgg cgtcgatatc gagatcaaga
     4801 tgtgaacccc ttcgggcggg ccgcctcggc ccgccgcgag ggcgttccgc catcaggaag
     4861 gaaccacgaa cagtgccggc aggtttgatc gggaggaagc tcgggatgac gcgcatcttc
     4921 ggcgacgacg gaagcgtcgt gccggtgagc gtcatcgagg ccaccccgaa cacgatcacg
     4981 cgcatccgcg acgcggcgaa ggacgggtac accgccctgc agctcggcgc gggaaccgcg
     5041 cgccgcgcga cgaagccggt ggccggccag ttcaagcacc tcgacaagga gcagcagcgc
     5101 ccgcgcaacg tgcgcgaggt gcgggtcgac tcggccgatg gctacgaggt cggccagcag
     5161 ctcggcgtcg acctcttcga ggcgggtgag ctggtggacg tgaccggcgt cagcaagggt
     5221 cacggcttca ccggcgtcat cgcacgccac aacttccgtc gcggccccaa gtcgcacggc
     5281 tccgaccacc accgggcgcc cggctccgtc ggtgccggca cgtacccggg acgcgtctgg
     5341 aaggggaccc gcatggccgg tcacatgggc aacgagcagg tgaccgtgaa gaagctcaag
     5401 gtcgtgaaga tcgatccgga gcgcaacctc atcctggtca agggtgcggt gcccggggcg
     5461 cgcaacggcc tgctgctcgt gcagaaggcg aagtaacgat gccccagacg acgatcctct
     5521 ccccggacgg cgcgaaggcc ggcacggtcg acctggccga gtcgctcttc gccgcaccga
     5581 tcaacgaaag cgtcatccac caggcggtgg tccgccagct ggccggacgt cgcgccggca
     5641 cctccgacac gcagacgcgc ggcgaggtgc agggcggcgg cgccaagccg tggcgccaga
     5701 agggcaccgg ccgcgcccga cagggaagcc ggcgtgcccc ccactggacc ggcggtggcg
     5761 tggtcttcgg accgcacccg cgcagctacg agcagaagat gccgaccaag atgcgccgca
     5821 gcgcgctgcg cggcgtgctg tccgccaagg cggcggatgg cgccctgcgc gtggtcgagg
     5881 gcttcgggct cgacgacatc aagacgaaga cgatgctcga gcgcctggcg ggctggaacg
     5941 ccgagggcaa ggtccttctg gtcctgcccg ctcgcgacga gcgcgtcgag ctctcggcgc
     6001 ggaacctgcg ccaggtccgc gtggtgctgg ccgacagcct caacgtggtc gacctgctcg
     6061 aggccgacac catcgtcttc accagcgacg cgctgagccg cgcccaggag gtgtacgcat
     6121 gaccgcgcag agcctgcacg acgtgctcct ccggcccgtc atcagcgaga agagcacgct
     6181 cgagaccgag cgcaacaact acacgttcgc ggtcgcccgc gacgccaaca agttcctcat
     6241 caaggcagcc gtcgaggccg agttcaaggt gaccgtgctc gacgtgcgaa tcgtcaacgt
     6301 ccgtcccaag cagaagcggc gcggccgccg gacgctgggc acggtgccgg gctggaagaa
     6361 ggccgtggtg acgatcgccg ccggggacaa gatcgagctc ttcgaggccg tctgaccatg
     6421 ccacttcgca gctaccgacc gacgtcgccg ggccttcgcc agatgacccg gtcgacgttc
     6481 gaggagatca cgaccgatac gccgcacaag ccgctcaccg agcggctggt ccggcgcgcc
     6541 ggccgcaaca gccagggcaa gctcaccgtc cgccaccagg gctcgggcca caagcgcctg
     6601 taccgggtga tcgactggaa gcgcgacaag ttcggcgtgc ctgctcgcgt cgcgaccatc
     6661 gagtacgacc cgaaccgctc ggcgcgcatc ggcctgctgc actacgcgga cggcgacaag
     6721 cgctacatgc tgctgccgca cggcatcaag gtcggcgaca ccgtcgcctc cggcccggac
     6781 gtcgaggcgc gcgtcggcaa cgccctgccg ctgtcgcgca tcccgctggg cacccaggtg
     6841 cacaacgtcg agctgtcgcg gggccgcggc ggccagatcg tccgctccgc ggggagcagc
     6901 gcgcagctgc tggccaagga gggcgagttc gccaccctgc gcctcccgtc cggcgaggtg
     6961 cgccgcgtgc gcgtggagtg catggcgacc atcggccagg tcagcaacct cgaccacgag
     7021 aaccagaaga tcggcaaggc cggtcgctcg cgccacatgg gcaagcggcc gaccgtgcgc
     7081 ggctcggcca tgaacccgaa cgaccatccg cacggtggtg gcgagggcaa gcagccgacc
     7141 ggcatgccgc cgaagaccaa gtggggcaag ccggccatgg gcctgcgg
//
LOCUS       DASTYP010000120         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_773777, whole genome shotgun sequence.
ACCESSION   DASTYP010000120 DASTYP010000000
VERSION     DASTYP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773777"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFV59_05555"
     CDS             <1..465
                     /locus_tag="VFV59_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HEU5325331.1"
                     /translation="DKLAPDVALMGMASDDARGSLVPGAVAAMRNVPLLSYGAELTLG
                     DGEARVRRLSSTGFDWVAAPLPVVASVTDQVGEPRYASLKGIMAAKRKPLETWSLADI
                     GLSADDLHGAALTKVTDARPPEAKPPTERVENLPAAEAAARIADWLAARKLI"
     gene            471..1451
                     /locus_tag="VFV59_05560"
     CDS             471..1451
                     /locus_tag="VFV59_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU5325332.1"
                     /translation="MSNDILFLAEFNGSAARSALELATGAAQLAAESGGSAVALAYGP
                     GASDGAAALGAQGAARAIVLGDDDRPAIGFAPAAAAAVRDADALALLAPATPNGRDIA
                     AALVGLLDLPAFGPVRAARVADGTIETEQATLQGSVITTSRPADGAPTPSVVLVLANT
                     FTPAEGGSGSASVEQAPGADDSALAKATVAERHEAEAKVVNLEEATVIVAGGRGVGSE
                     EGFGPLRELASALGGAVGASRAAADAGWIPYQLQIGQTGKVVKPSLYLGVGISGAIQH
                     RVGMQTAEHVVAINKDPDAPIGEFADLFVVGDLFQVVPALTDEIKRRKGA"
     gene            1460..1975
                     /locus_tag="VFV59_05565"
     CDS             1460..1975
                     /locus_tag="VFV59_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325333.1"
                     /translation="MRNLFGGRRRSVFGHGKELATYRNTLFELTTRIDARLGAVADDM
                     DALRRRAIEPEEAVRRLTVHEDALDANEQELEGMLAPEDLHGLHMEYEANLERALRGI
                     VTVERGCAITRLPHRPPEDEEPFTYYKRGHANVTHARMRMAEIVEVLLKWEPGKPADA
                     SLTVRLRQDEG"
     gene            1980..2939
                     /gene="ligD"
                     /locus_tag="VFV59_05570"
     CDS             1980..2939
                     /gene="ligD"
                     /locus_tag="VFV59_05570"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU5325334.1"
                     /translation="MPAASDPESRDGVALTNLDQPLFDGAGATKRDLVDYLDAIADRI
                     LPVLRGRALSVIRVHRGQEAFMQKNVPRYTPDWVRTVRVWSESSRRDVDYALCDDRRT
                     LLWFANQRAVEYHPTLVRADRPDRATHIVLDLDPPAEGGFAMAVRAAYLVRQALTDAG
                     LEGVAKTSGAKGVHVFVPIDRRATIDEAAAATRAIAERAARLDPEIATTAFLKEDRGG
                     RVFVDSTRAGGATVVAAYSPRVRPGAPVSFPVHWDELDGVAPGDFTIHTALDRLGSAD
                     PWAELMPRAQRLPAALIAEGRAIPTARVQAMHEGKRRARARRG"
     gene            3026..3490
                     /gene="nrdR"
                     /locus_tag="VFV59_05575"
     CDS             3026..3490
                     /gene="nrdR"
                     /locus_tag="VFV59_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU5325335.1"
                     /translation="MRCPRCEQTGTRVIDSRDLEGGSTIRRRRECEHCAYRFTTYERP
                     EGARLVVVKRDGSREDFDREKVRAGMLKALQKRPVTLDRVEAAVDGIETQLRARGDAE
                     VASTEIGRLATDALRELDQLAYIRFASVYHAYDDIETLQREVDRLIRQRAGS"
BASE COUNT          510 a         1326 c         1279 g          467 t
ORIGIN      
        1 gacaagctgg cgccggacgt cgccctgatg ggcatggcct cggacgacgc gcgcggctcg
       61 ctggtgcccg gcgcggttgc cgcgatgcgg aacgttccgc tgctctccta cggcgccgag
      121 ctgacgctcg gtgatggcga ggcgcgcgtg cgccgcctct cgtcgaccgg cttcgactgg
      181 gttgccgcac cgctccccgt ggtcgccagc gtgaccgacc aggtcggcga gccccgctac
      241 gcgagcctta agggcatcat ggccgcgaag cgcaagccgc tcgagacgtg gtcgctggcc
      301 gacatcggcc tctctgcgga cgacctccat ggcgccgcgc tgaccaaggt caccgatgcg
      361 cgcccgcccg aggccaagcc accgaccgag cgcgtcgaga acctgcccgc ggccgaggcg
      421 gccgccagga tcgccgactg gctcgccgca cgaaagctga tttgagaccg atgagcaacg
      481 acatcctctt cctcgccgag ttcaacggct cggccgcccg atcggccctc gagctcgcca
      541 ccggtgccgc ccagctggcg gccgagtccg gtggctcggc cgtcgccctc gcctacgggc
      601 ccggtgcctc cgacggcgcc gccgccctgg gcgcccaggg tgcggcgcgc gccatcgtgc
      661 tgggtgacga cgaccggccg gccatcggct tcgcaccggc cgccgccgcc gcggtgcgcg
      721 atgccgatgc gctcgccctc ctggccccgg ccacgcccaa cggccgcgac atcgctgcgg
      781 cgctggtggg gctgctggac cttcccgcct tcgggccggt ccgtgcggcc cgggtggccg
      841 acggcaccat cgagacggag caggcgacgc tgcagggcag cgtcatcacc acctcgcggc
      901 cggccgacgg cgcgccgacc ccctcggtcg tgctggtgct agccaacacg ttcacgccag
      961 cggagggcgg cagcggcagc gcatcggtcg agcaggcgcc gggcgccgac gactcggccc
     1021 tggcgaaggc gaccgtcgcg gagcgccacg aggcggaggc gaaggtcgtc aacctcgagg
     1081 aggcgacggt gatcgtggcc ggcggccgcg gcgtcggcag cgaggagggc ttcggtccgc
     1141 tgcgcgagct cgcctcggcc ctgggtggtg ccgtgggcgc gtcccgggcg gccgccgacg
     1201 ccggctggat tccgtaccag ctgcagatcg ggcagaccgg gaaggtcgtg aagccgtcgc
     1261 tgtacctggg cgtcgggatc agcggtgcga tccagcatcg ggtcggcatg cagaccgccg
     1321 agcacgtcgt ggccatcaac aaggatcccg acgcgccgat cggcgagttc gcggacctgt
     1381 tcgtggtcgg cgacctgttc caggtcgtgc cggcgctgac ggacgagatc aagcggcgga
     1441 agggcgcctg agcctcgtga tgcgcaacct cttcggcggc cggcgacgca gcgtcttcgg
     1501 ccacggcaag gagctcgcga cgtaccggaa cacgctcttc gagctgacca cccggatcga
     1561 cgcccgactt ggcgccgtgg ccgacgacat ggatgccctg cgccgccgcg ccatcgagcc
     1621 ggaggaggcg gtgcggcgtc tcaccgtcca cgaggacgcg ctggacgcga acgagcagga
     1681 gctcgagggc atgctggccc ccgaggacct gcacggcctg cacatggagt acgaggccaa
     1741 cctcgagcgc gcgctgcgcg gcatcgtcac cgtcgagcgc ggctgcgcca tcacgcggct
     1801 gccacaccgc ccacccgagg acgaggagcc gttcacgtac tacaagcgcg ggcacgccaa
     1861 cgtgacgcac gcgcggatgc ggatggcgga gatcgtggag gtgctcctga agtgggagcc
     1921 cgggaagccc gccgacgcgt cgctcacggt gaggctgcgc caggacgagg gctaggcggg
     1981 tgccggccgc atccgacccg gaatcccgcg acggggtcgc gctgaccaac ctcgaccagc
     2041 cgctgttcga cggcgccggc gccaccaagc gcgacctggt cgactacctc gacgccatcg
     2101 ccgaccgcat cctgcccgtc ctgcgcggac gggcgctctc ggtcatccgg gtccaccgcg
     2161 ggcaggaggc gttcatgcag aagaacgtcc cgcgctacac gcccgactgg gttcgaaccg
     2221 tgcgcgtctg gtcggagagc tcgagacgcg acgtcgacta cgccctgtgc gacgaccggc
     2281 gcacgctgct gtggttcgcc aaccagcgcg ccgtggagta ccacccgacg ctggtgcggg
     2341 ccgatcgccc ggaccgcgcg acgcacatcg tcctcgacct cgacccgccg gccgagggcg
     2401 ggttcgccat ggcggtgcgt gccgcgtacc tggtccgcca ggcgctgacc gatgcgggcc
     2461 tcgagggcgt ggcgaagacc tccggcgcca agggcgtgca cgtcttcgtg ccgatcgacc
     2521 ggcgcgccac catcgacgag gccgccgccg cgacccgcgc catcgccgag cgcgccgccc
     2581 gcctcgatcc cgagatcgcg accacggcct tcctcaagga ggaccgtggc ggccgggtgt
     2641 tcgtcgactc cacgcgcgct ggcggcgcga ccgtggtagc ggcctacagc ccgcgcgtcc
     2701 ggcccggcgc gccggtctcc ttcccggtcc actgggacga gctggacggc gtcgcacccg
     2761 gcgacttcac gatccacacg gccctcgacc ggctgggcag cgcagacccc tgggcggagc
     2821 tgatgccgcg cgcgcagcgc ctgcccgcgg ccctcatcgc cgagggccgc gccatcccga
     2881 ccgctcgggt gcaggcgatg cacgagggga agcgccgagc ccgcgcgcgt cgcggctagg
     2941 cccacggcgc tcggcaatgc cacatcttgt ggtatgctcc gcgtcggtac cactccttgt
     3001 gtagtccgca ccgaacggga gctgcatgcg ctgtccacgc tgtgaacaga ccgggacgcg
     3061 ggtcatcgac tcgcgcgatc tcgagggggg cagcaccatt cgtcgccggc gcgagtgcga
     3121 gcactgcgcg tatcggttca ccacgtacga acggcccgag ggcgcgcgcc tggtcgtcgt
     3181 caagcgcgac ggctcgcgcg aggacttcga ccgcgagaag gttcgggcgg ggatgctcaa
     3241 ggcgctccag aagcgtcccg tgaccctcga ccgggtcgag gccgccgtgg acggcatcga
     3301 gacccagctg cgggctcgcg gcgatgccga ggtcgcctcg accgagatcg gccgcctcgc
     3361 caccgatgcg ctgcgcgagc tcgatcagct ggcgtacatc cgcttcgcat ccgtctacca
     3421 cgcctacgac gacatcgaga ccctccagcg agaggtggat cggctgatcc gtcagcgcgc
     3481 cggatcgtaa gatcagcgcc gcatgatcct gtccgaccgc gacatccgcg ccgagatcga
     3541 ggccggccgg atcgtcatcg atccgttcgt gcccgatgcc gt
//
LOCUS       DASTYP010000121         4750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1319470, whole genome shotgun sequence.
ACCESSION   DASTYP010000121 DASTYP010000000
VERSION     DASTYP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319470"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..2428)
                     /locus_tag="VFV59_05580"
     CDS             complement(8..2428)
                     /locus_tag="VFV59_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5325336.1"
                     /translation="MGFGTTNGHPSGNGNGSASGNGHRPVERDGHVTDYTPIWPMVPG
                     TRTISVTINGQQVEAEEGQTILQACRVLGIDIPTLCYEPKLAPYGACRICAVEVEGVE
                     GTPISCGTTITEGMVITTHSDRVLENRRMVLELIFSDHDAYCLPPCQFKCPTRVDIPG
                     YLKQNTLGNWEEATRILKRSLPFPAILGRVCPAPCETHCRREEVDTAIAIRDSHRYCA
                     DRVLEVDAPAPIPWPKEPATGRKVAIIGAGPAGMAAAYYLQLRGHQCDVFEAEPEQGG
                     MLRYGIPQYRLPKGWMDRELNHVWELGAQFRPNMRLGRDFHIADLLPEYDAVYVGIGC
                     YRSNELGIPGEDADGMVNALENLYNSTRGVPVPRLKGARVVVIGGGFTAIDCTRTSVR
                     QGAAEVTLVYRRDLKDMPAADEAHEAIEEGARLIFQAAPTRILTDENNKVTGVEFQRM
                     ALGEPDEKGRRRPEPRPGTEFVVECDTVLGAIGQGPDLSWIETEPEELRSQLEVSRRG
                     TLVAEEHIFRTNVPKVFASGDVRTGAATVVEAIGEGRRASYAIDYWLRGHDLDDPQVR
                     RIVTEPQPNFLTIVPFTDDVKEPRAIMGKLGPEGRRDNFSEYEFGYTGEQAMAEASRC
                     LQCTCEAIGHCDLREAAIEYETTLNMAIDERSIQDNPYVGVNHGYGRDETHSFILRDY
                     SRCIDCGRCAQVCKEIVGAGCYDFIGKGFDSLVTTADYVSLNETPCVSCGRCAETCPV
                     GALMPRPRTLETYQLDISRCIFCGICADACPYDALRCGPEFEFSEYQRDLPMLDILEM
                     SDRERPTR"
     gene            complement(2435..3802)
                     /locus_tag="VFV59_05585"
     CDS             complement(2435..3802)
                     /locus_tag="VFV59_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5325337.1"
                     /translation="MANTLSPQIRILDAGERRILTDVGRIDPGSIASFREHGGYAGLE
                     RAVTRMSPEQVIAEIRTAGLRGRGGAGYPTADKWEAVRATPAERKIVIANLMGADPSA
                     LGDRALAEGNPHLVLEGLLIAAFATGAAEAIVAVRREWTVAIERLRAAAADATAARLA
                     GYLVLGTDTSIQLSIWEGSGAYVAGEETALISALAGDRGMPLIRPPYPARTGLSGAPT
                     AVQNAETLAHVAWIVSHSAEAFASVGSEASRGTKLVTLMGKVAQPGLLEVPMGTPLID
                     VLGMAGGGVGATKALFVGGPSGGAIDAGSLNTPYEYESLEAAGAIVGSGSILVTDTST
                     CMVDTARFFVDFSAREACGKAVPCRIGTRRLVEALDRILAATPRPNDFVLLRELSRKV
                     SDTALCQLERLAPRPILTTLERFADEYRAHAERGECLAGACRTEGLAPLLQPLTGIDA
                     APRVE"
     gene            complement(3802..4647)
                     /locus_tag="VFV59_05590"
     CDS             complement(3802..4647)
                     /locus_tag="VFV59_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU5325338.1"
                     /translation="MGFPGIGIAKGMATTFKNFFTPKVTRQYPEERPPIPDRWRGRLD
                     LIYDPFGDHKCEVCFQCAQVCPVEAIDMSGFDSQGNRIRYGMPEIYDERRDPNAYRRA
                     GLPARPMRNPARWDEAIDTAFVDEVVDAYGAQPAALVAIFRGVQERFGYLPERALRRI
                     SDRLVIHWAQVFGAASLGGFRLLPTEGHLITVCDCASCRFAGGDRILRAIEDDLGIRP
                     GETTADGRFTLETRTDVGAGAISPAVRIDTLVYGPVTAEQVTHLVDERRRGAAAAGAT
                     AGAAS"
BASE COUNT          654 a         1636 c         1737 g          723 t
ORIGIN      
        1 cgaagcgtca gcgggtgggg cgctcccggt cggacatctc gaggatgtcg agcatgggca
       61 ggtcgcgctg gtactcgctg aactcgaact ccggaccgca gcgcagggcg tcgtacgggc
      121 aggcgtcggc gcagatgcca cagaagatgc agcgcgagat atccagctgg taggtctcga
      181 gcgtccgcgg ccgcggcatg agggcgccga ccgggcaggt ctccgcgcat cggccacagg
      241 agacgcacgg cgtctcgttg aggctcacgt agtccgcggt cgtgacgagc gagtcgaagc
      301 ccttgccgat gaagtcgtag cagccggcgc cgacgatctc cttgcagacc tgcgcgcagc
      361 ggccgcagtc gatgcagcgc gagtagtccc gcaggatgaa ggagtgggtc tcgtcgcggc
      421 cgtagccgtg gttgacgccg acgtacgggt tgtcctggat cgagcgctcg tcgatcgcca
      481 tgttgagcgt cgtctcgtac tcgatcgccg cctcgcgcag gtcgcagtgg ccgatcgcct
      541 cgcaggtgca ctgcaggcag cgtgacgcct cggccatggc ctgctcgccg gtgtagccga
      601 actcgtactc gctgaagttg tcgcgccgac cctcggggcc gagcttgccc atgatggccc
      661 gcggctcctt cacgtcatcg gtgaagggca cgatggtcag gaagttgggc tgcggctcgg
      721 tcacgatgcg ccgcacctgt ggatcgtcga ggtcgtggcc gcgcagccag tagtcgatgg
      781 cgtacgaggc gcgccgcccc tcgccgatgg cctcgaccac cgtggccgca ccggtccgga
      841 cgtcgccaga ggcgaagacc ttcgggacgt tggtgcggaa gatgtgctcc tcggcgacca
      901 gcgtgccacg gcggctgacc tcgagctggg agcggagctc ctccggctcg gtctcgatcc
      961 acgacaggtc cggcccctgg ccgatggccc ccagcacggt gtcgcactcg accacgaact
     1021 cggttccggg gcggggctcc ggccgacggc ggcccttctc gtccggctcg ccgagcgcca
     1081 tgcgctggaa ctcgacgccg gtcaccttgt tgttctcgtc ggtcaggatg cgggtcgggg
     1141 cggcctggaa gatgaggcgc gcgccctcct cgatggcctc gtgggcctcg tcggccgcgg
     1201 gcatgtcctt caggtcgcgg cggtagacca gcgtgacctc cgcggcgccc tggcgaaccg
     1261 aggtccgggt gcagtcgatg gccgtgaagc caccgccgat gacgaccacc cgcgcgccct
     1321 tcaggcgcgg caccggcacg ccgcgggtcg agttgtagag gttctcgaga gcgttgacca
     1381 tgccgtccgc gtcctcgccg ggaatgccca gctcgttgga gcggtagcag ccgatgccga
     1441 cgtagaccgc gtcgtactcc ggcaggagat cggcgatgtg gaagtcgcgg cccagccgca
     1501 tgttcgggcg gaactgcgcg ccgagctccc agacatggtt gagctcgcgg tccatccagc
     1561 ccttcggcag ccggtactgc gggatgccgt aacggagcat gccgccctgc tcgggctccg
     1621 cctcgaagac gtcgcactga tggccgcgca gctgcaggta gtaggcggcc gccatgccgg
     1681 ccgggcccgc accgatgatc gcgaccttgc ggccggtggc gggctccttc ggccatggga
     1741 tcggggccgg ggcgtcgacc tcgagcaccc ggtcggcgca gtagcggtgc gagtcgcgga
     1801 tggcgatggc cgtgtcgacc tcctcgcgcc ggcaatgcgt ctcgcacgga gcgggacaga
     1861 cgcggccgag gatggcgggg aacggcagcg agcgcttcag gatgcgcgtc gcctcttccc
     1921 agttgcccag ggtgttctgt ttcaggtagc ccgggatgtc gacgcgggtc gggcacttga
     1981 actggcaggg tggcaggcag taggcgtcgt gatcgctgaa gatcagctcc agcaccatgc
     2041 gccggttctc gaggacccga tcggaatggg tggtgatgac cattccctcg gtgatcgtcg
     2101 tgccgcacga gatcggcgtg ccctcgacgc cctccacctc cacggcgcag atgcggcacg
     2161 cgccgtacgg tgcgagcttc ggctcgtagc agagggtcgg gatgtcgatg cccaggacgc
     2221 ggcaggcctg gaggatcgtc tggccttcct cggcctcgac ctgctgtccg ttgatcgtta
     2281 ccgagatcgt ccgcgtgccg gggaccatcg gccagatcgg cgtgtagtcg gtcacgtggc
     2341 cgtcgcgctc gaccggccgg tggccgttgc cgctcgcgct gccgttgccg ttgccagacg
     2401 gatgtccgtt cgtcgttccg aagcccatcg gttcctactc tacccggggt gctgcgtcga
     2461 tgccggtgag cggctggagg agcggcgcca ggccctcggt gcggcacgcg ccggcgaggc
     2521 actcgccgcg ctcggcgtgg gctcggtact cgtccgcgaa ccgctcgagc gtcgtcagga
     2581 tcggccgcgg cgccaggcgc tccagctggc atagggcggt gtcgctcacc ttgcgcgaca
     2641 gctcgcgcag caggacgaag tcgttcggac gcggtgtcgc ggccaggatg cggtcgagcg
     2701 cctcgaccag ccgccgcgtc ccgatgcggc acgggaccgc cttgccgcag gcctcgcggg
     2761 cgctgaagtc gacgaagaag cgggcggtgt cgaccatgca ggtcgacgta tcggtcacca
     2821 ggatcgagcc cgatccgacg atcgcccccg ccgcctccag cgactcgtac tcgtacggcg
     2881 tgttgagcga tccggcgtcg atggcgccgc ccgagggtcc gcccacgaag agggccttgg
     2941 tcgccccgac cccgccgccg gccatgccca gcacgtcgat cagcggcgtg cccatcggca
     3001 cctccaggag gccgggctgg gcgaccttgc ccatgagggt caccagcttc gtcccgcggc
     3061 tcgcctcgct gccgaccgag gcgaaggcct ccgcggagtg cgacacgatc cacgcgacgt
     3121 gggccagcgt ctcggcgttc tgcaccgccg tcggcgcacc cgacaggccc gtgcgagcgg
     3181 ggtacggagg ccggatgagc ggcatgccgc ggtcgccggc cagggcgctg atcagcgccg
     3241 tctcctcgcc cgcgacgtag gcgcccgagc cctcccagat cgaaagctgg atgctggtat
     3301 cggtgccgag caccaggtag ccggccaggc gtgcggcggt cgcgtcggcc gcggcggcgc
     3361 gcagccgctc gatggcgacc gtccattcgc gccggaccgc cacgatggcc tcggcggcgc
     3421 cggtggcgaa ggccgcgatc aggagccctt cgagaacgag gtgcgggttg ccctccgcca
     3481 gggcgcggtc gcccagggcg ctcggatcgg cgcccatgag gttggcgatg acgatcttgc
     3541 gctcggccgg cgtggcgcgc acggcctccc acttgtcggc ggtcgggtag ccggctcccc
     3601 cacgcccgcg caggcccgcc gttcggatct cggcgatgac ctgctcgggt gacatccgcg
     3661 tcaccgcgcg ctcgagcccg gcgtacccac cgtgctcgcg gaagctggcg atggagccgg
     3721 ggtcgatgcg accgacgtcg gtgaggatcc ggcgctcacc ggcgtccagg atgcggatct
     3781 ggggcgagag ggtgttcgcc atcagctggc cgccccagcg gtcgcgcccg cggcagccgc
     3841 accacggcga cgctcgtcga cgaggtgcgt gacctgctcg gccgtcaccg gcccgtacac
     3901 cagcgtgtcg atgcggaccg ccgggctgat ggcgcccgcc ccgacatcgg tccgggtctc
     3961 cagcgtgaag cggccgtcgg cggttgtctc cccgggcctg atgcccaggt cgtcctcgat
     4021 ggcgcgcagg atccggtcgc cgccggcgaa gcggcacgag gcgcagtcgc agaccgtgat
     4081 caggtggccc tcggtcggga gcaggcggaa gccgcccagg ctggcggcgc cgaagacctg
     4141 cgcccagtgg atgaccaggc ggtcgctgat gcggcgcagc gcgcgctcgg ggaggtagcc
     4201 gaaacgctcc tggacgccgc ggaagatggc gaccagcgcc gccggctgcg cgccgtaggc
     4261 gtcgaccacc tcgtccacga aggccgtgtc gatcgcctcg tcccagcgcg ccgggttccg
     4321 catcgggcgg gccgggaggc cggcgcggcg gtaggcgttc gggtcacgcc gctcgtcgta
     4381 gatctccggc atgccgtacc ggatccggtt gccctgggag tcgaagccgc tcatgtcgat
     4441 ggcctcgacc gggcagacct gcgcgcactg gaagcagact tcgcacttgt gatccccgaa
     4501 cgggtcgtag atcaagtcca ggcggccgcg ccagcgatcg gggatcggcg gccgctcctc
     4561 ggggtactgg cgcgtgacct tcggcgtgaa gaagttcttg aaggtcgtgg ccatgccctt
     4621 ggcgatgccg atgccgggga atcccatcgg tcctgcgcag cctccctaca ggtccagcac
     4681 gaccaccgcg atggcggtca cgaacaggtt cacgagcgcg agcggcagca ggaccttcca
     4741 ggccatgccc
//
LOCUS       DASTYP010000122        19844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_956031, whole genome shotgun sequence.
ACCESSION   DASTYP010000122 DASTYP010000000
VERSION     DASTYP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 19844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19844
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956031"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFV59_05595"
     CDS             complement(<1..644)
                     /locus_tag="VFV59_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEU5325339.1"
                     /translation="MKTFPRLLRQRPRGAGTEIVGEEPRGEPRPEIIEAPTLRWINID
                     EPRLADRAWLAEEFGFHELDLEDVASHNQRPKVDEYADYLFLVMQFPRYDKESGRLHA
                     AELDVFVGPNYVITLPNEEILPLPSLFERTAAREDVRQQLMTKGSGYLLYEILDRCVD
                     ASFPMLGALGRKLRRLEDDIFEGGASSEIVREISNAKQEIINFRAVIRPQRAVFR"
     gene            770..2521
                     /gene="polX"
                     /locus_tag="VFV59_05600"
     CDS             770..2521
                     /gene="polX"
                     /locus_tag="VFV59_05600"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEU5325340.1"
                     /translation="MPRPRSVSRSGDAVISNAELARIFTEIGDMLEILGEVVYKAVAY
                     RRVADAIERYPDDVAALFRRGTPPKLPGAGSALTAKLAELSETGRLEYHERLRAQVPA
                     GLLDMLRVPGIGPKTVRLLHAELGIDSVEGLRAAAEQGQLRHVKGLSERTEQNVLEAI
                     GRIERRSTRLLIHDADALVATLLADLADVPGVRRIERAGSLRRRRSTIGDLDLLAAVD
                     DPVAVIARLDGLPEVEKVLSAGTDKSSIVLREGPQVDLMVCPPDAWGTHLVHFTGSKD
                     HNVALRGMALDRGWSLSEKGFKVIETGELLLEADEEAVYARLGMPWIAPELREGDGEI
                     EAALAGALPELVTVDDVRGDTHTHSDWTDGVDSIETMARAARDLGHEYMVLTDHSPSL
                     GITRGLPPARVEEQRAEIQRLNGELAPFRILHGTEMEIRADGSLDYPDALLARYDVVV
                     ASIHTGRGQPAEQLTRRALAAIENPHVDILAHPAGRIVNRRDPLPLDWPRVFEAAART
                     GTALEINGSPRLDLEDSLARAAGRAGARLTLASDAHRTEELGQQRYAVDVARRAWLTA
                     DQLLATRSADDLLELLR"
     gene            2518..3294
                     /locus_tag="VFV59_05605"
     CDS             2518..3294
                     /locus_tag="VFV59_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325341.1"
                     /translation="MITRLVKDRRSLAVLRRIDRPAAVLLAIAQAVTAAVGWFVDPIW
                     LAVTVAVQLALGGIGAVRVIGAGPAELGFARYAMPATAGIAATLVGRLLPGGLSLLLV
                     PIVAVLLWSVWYLELRLARGSGGLTIGGLLHTVILFSAAAGLLDLFGPKAWPTPLILV
                     AVFALPVALRSAEARGAVGAEGVGQALLHLLVVMEIGAGAVLLDLPITILAALMALAF
                     YAWAGAVDALRGGTSGRAVAIEYGAFVLLGLLVALLLHRS"
     gene            3391..4236
                     /locus_tag="VFV59_05610"
     CDS             3391..4236
                     /locus_tag="VFV59_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5325342.1"
                     /translation="MLATLMALGEADTTTRQRRQLGEAAIAQATRGEWDAAVATNRQL
                     LELGPDIDAYNRLGKALAELGRNEEAVEAYEQALERDATNRIAQRNVERLRLVIAAAA
                     KTDGKGRAEKAPATLFIEEMGKTGRARLLNLAGPRVLAPLSPGDAVELGAENDELVAR
                     VGGELIGSVEPRIGSRLLKLMAGGNRYEAAITTIHEAGGELSIIIREVFTHPSNFGKV
                     SFPTVSQGGGGVRPYMKGSALRYDDDEEVEEVDEESEEVEELDTTLPEYSGEPDLEEE
                     LVEEP"
     gene            4241..7015
                     /locus_tag="VFV59_05615"
     CDS             4241..7015
                     /locus_tag="VFV59_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325343.1"
                     /translation="MRDAERTTLDHVVLPRRRPGLPLLERLFPGPAENLLDAELAAFA
                     TEGDTVLDPWAGAGWTARRTVQRGLHAIAADASPLAQLAGVTLLTAPDPIALDAAFAQ
                     LAGSRRVDVPLRQHLEELYASRCRACRRPVVVDQFIWPRDGDAPGRKIYRCAACEAAL
                     GGGEVDRVAPVDEIDLAKLGIDAGADASVAEETAPFAAAALDAAGVEDPDAPLGEAGG
                     PPLPPSPPVDPPVGEASRPRFASTVRPDPVPVGSAEGIRHAPQYLDLVARFPVLDGRH
                     DLVAELLDLYTPRNLYALHAIGSKIDSEVHDSGAAGALRLALASVLLPASRLNGYPGR
                     VAGLRISGGHVRQPASRHQREINVWRAFEEAYREVRAVVADAARDRRPARFAADAGEL
                     GGMEAANVLWLRTRPGVVAQYLPAEGIDFVLSAPAPPRAHDELSFEYLATSWILGREA
                     AETLPLESVFAGTSPPAGAEATAVRHGLASAAAALKPGGRCSLILEGADPDRLVTFAQ
                     AAAAVGFEISDLVYRESRRSGDGLVLHLRKPSAEDRLRAAAQPGPLRLGVADGHLTYP
                     ELASAIQRAVVDLLRDRGEPAGLLRVVSAITLELARAGLLRRFATTRGGGEADADAAD
                     DEPRTLDAGPHALATLIREELWRDEHPALVRIGDDSDPQWWLRDPELAQAPLADRVER
                     AAWSVLSTAGRIDEDGFLDRIYALFPGLAAPDEELVRATLEAYAVPGERGSLGTDDDI
                     ASRTEDHARVIARLVEYGHRLGLRTWIARREQERTVAGRPLLALLSDEERRVYLPLVV
                     RGAADALGAVDVIWYVRGKLAFLFEVEWTAAVGETILGRGREVPVGEQQARFLVLPAR
                     RAELLRLKLQRSPWLRAEVERQNWHVLKWEHLETLAARPGARLEWLEPVLGLDPLIER
                     GGEQLTMFGE"
     gene            7137..7865
                     /locus_tag="VFV59_05620"
     CDS             7137..7865
                     /locus_tag="VFV59_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5325344.1"
                     /translation="MSPLERHELISPLERALVVISHPDDAEFGAAPTIAMLTRAGVRV
                     DYVVTTDGSKGTEDRDVAPQQLAATRMAEQRDAADILGVTDIVHLGYPDGYLTPSLDL
                     RRDITRQIRRYRPDLVITQNPQRRLDHNPFIGHPDHLATGEATLASVYPAARDHLNFP
                     ELYSDEQLEPWKVRQVLLTGVEEPNLWLDVSETFDVGIRSILAHVSQVDPGTVEERMR
                     ERARIIGEPQGIGLAQAFLSIILT"
     gene            7862..9781
                     /locus_tag="VFV59_05625"
     CDS             7862..9781
                     /locus_tag="VFV59_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325345.1"
                     /translation="MTRRGGGGGGPWGRFGAPEPDLTRRPGSERQTLRRIAGFFRPYR
                     GRLTVIAVLILITVSVGVVNPILIKLVLDNLLPGGPQDLDLLYLQVGLMVVLPIITSA
                     IGVWQSYLSNVVGQRVMEDLRLALYRHLQWMPLRFFTETRTGEIQSRISNDVGGVQSV
                     VTDTAASLLSNFATVATTVIAMLVLDWRLTLLSLGLLPVFAYITYRVGKVRREVSTLT
                     QRSLAEVSAITEESLSVSGILLSKTFGQQEASIERFRRESRRLGDLQIRGQMIGRWFF
                     ALIGTFFSIMPAFVYLLAGSLIIGGDTNISIGTIVAFTTLQSRLFFPLGQLLNVQVEI
                     QGALALFDRIFEYLEMEHDITDAPDAVALAPATMRGEVRFDHVTFRYPVAAAQLHAAI
                     ETAEAEAEGRAVPTQIQPFALEDISFTARPGQLVALVGPSGSGKTTSTYLIPRLYDVD
                     AGSVSIDGVDVRGIRLESLGRVIGFVTQETYLFHDTVLANLRYAKPEATMEEIEEAAR
                     AAAIHQRITELPDGYDTVVGERGYKLSGGEKQRVAIARVLLKDPRILILDEATSALDT
                     VSERLIQAALQRLMEGRTTIAIAHRLSTILRADQILVMQRGRIVERGTHDELLRHEGL
                     YARLSAEQFGPGSAA"
     gene            9823..10368
                     /locus_tag="VFV59_05630"
     CDS             9823..10368
                     /locus_tag="VFV59_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5325346.1"
                     /translation="MTMEMGMLEGLHGSTAQADADRLAVVDLQARRGPELYGFARRLG
                     LGPEAAEDAVQETLLRAWTVLADRSPVRDLDAWCFTTLYRLSMDQHRWRRRVQRLAER
                     LRPAPPRSESASVADRLTLWDAVDRLPERQRTVVQLRYRADLTFEQIAQVLHIKPVSA
                     RSHASRALDALASLLAEEDFR"
     gene            10365..11105
                     /locus_tag="VFV59_05635"
     CDS             10365..11105
                     /locus_tag="VFV59_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325347.1"
                     /translation="MTRDELERALRSSVDPHERTYRAAPLPASVEEARSRIGVRRTNG
                     GSALRFAGVVVATALAVVAVAAGYGLVTGGRGTGGDASASDASMVPITTPAASGSVAA
                     RPCRAADFAITSDPWDAGAGSRGTRVVFRAADSLDACVLGGRLAARIVDAQDEVLVAA
                     ESAETPTTTVTGGVQLEIGVRWSNWCGAAPREPLELRVRFPSDSAEVALVPPAGTSIL
                     VPPCMGGGEGTALSLTEFQASDRPAPEG"
     gene            complement(11121..11273)
                     /locus_tag="VFV59_05640"
     CDS             complement(11121..11273)
                     /locus_tag="VFV59_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325348.1"
                     /translation="MACRTTAFWALAHWTSRSAAQGFYEAWGTADEPGEEGWRLEGDV
                     GLVPLG"
     gene            11315..13381
                     /locus_tag="VFV59_05645"
     CDS             11315..13381
                     /locus_tag="VFV59_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5325349.1"
                     /translation="MPNRLADQTSPYLLQHAHNPVDWYPWGPEALERARAEDRPIFLS
                     IGYAACHWCHVMERESFENSEIAALMNREFVAIKVDREERPDLDDVYMAAVQAMTGSG
                     GWPMSVFLTPDLRPFYGGTYFPPEDRHGMPGFPRVLEAVADAYRNRRSEIEQQAGMLA
                     SHLASQLAVAPGRSEPQWQQLDAATTRLAASFDDVHGGFGSAPKFPPPMTLEFLLRSW
                     HRTGSARALRMVTRTLDAMADGGIHDQLGGGFARYSTDAHWLVPHFEKMLYDNALLAH
                     AYLEAHRATGTDRYARVARDTLDFMLRELRTADGGFASALDADSEGVEGRFYAWTDAE
                     LRSALADAGFGADERAALAAYWGVEPAGNWEGTNVLHVSDPGAADATLVERARQALLE
                     ARERRTRPARDDKQLASWNGLALRAFARAALVLDGPSYLAVTAELVEFVERHLVRDGG
                     RRLWRTVRDGQAHTPAFAEDYLAVADGLLDAHAALGTAAPLLLARTLVSTAVAEFWDD
                     EAGTFVDTSSEHERTVAQPRGMVDNAVPSANGLGADVLQRLALLTGDEDLARRARAIL
                     SAVAPALDRQPSAFGRMLGAADRLLGEPIDVVVAWTDADRGQPLRVAAMRPYVPDLVL
                     TSVGGGDEHASWPLYAAKVARADRATAYACRGYACDEPTDDAGRLVEQVRGLARAAAE
                     VSRPGS"
     gene            complement(13362..13772)
                     /locus_tag="VFV59_05650"
     CDS             complement(13362..13772)
                     /locus_tag="VFV59_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU5325350.1"
                     /translation="MQHREITLESGSQPAVVDLTDACVRFLADVHARDGLLTVFVPHA
                     TAGVVIMELGAGSDDDLLASLERLLPRDERWRHRHGSPGHGADHVLPLLVAPSVSVPV
                     VGGRMTLGTWQSVALVDPNRDNPRRTVQLSFLAG"
     gene            complement(13772..14632)
                     /gene="ccsA"
                     /locus_tag="VFV59_05655"
     CDS             complement(13772..14632)
                     /gene="ccsA"
                     /locus_tag="VFV59_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5325351.1"
                     /translation="MTELITPLLVAALAVLLATAAAGFVVAVLAPRPLATTGGADVDA
                     PRPPLAGLLVPGVGLGAGLLVAASVARAVAAGHAPWSNLHEVSIAFAAMLLLAYLALA
                     RSVPISGLAPLAAVLGAGLITVALNLDDRVQPLVPALQQPLLLTVHVGTAILAYALAG
                     VAFLAAAAELGQRAAGGRIALLPSVAVLRTVGHRAVLIGFPILTVAIVLGSVWANLAW
                     RSYWSNDPKELAAAATWLVFGAYLHVATRTDGWGRLAPWLIVVGFAGVLFTYLGAGLL
                     IVGEHSYGRP"
     gene            complement(14672..15085)
                     /locus_tag="VFV59_05660"
     CDS             complement(14672..15085)
                     /locus_tag="VFV59_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5325352.1"
                     /translation="MEFIRNAIEWIAADSPKLLFDLFPFLMLGVVGLFLLWLLIGYLR
                     VSQVGIHDGQAVQQAVALPPATDPNALPAAPQGVPYCAFDGLAYPLGARFCTACERDL
                     VLDCATCGATLSAGDASCYRCGTPTGVDAAAHHLA"
     gene            15163..16284
                     /locus_tag="VFV59_05665"
     CDS             15163..16284
                     /locus_tag="VFV59_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU5325353.1"
                     /translation="MPASRLDPRTPLTELHVHLGAAVTPPIMWGIAHAQGIRLPTKDY
                     WAFRDLITVGRRKRSFQAYLDLFHWTELIQSSPLAVERSVYEVIGGAYRKNNITQLEL
                     RFNPMKRNRGGEQDLDHIIAAAVRGMDRAVLEYPQVRAGLVFCLDRGFSRELNEIIAA
                     KAIAWHSRGVVGIDIAGPVVDRFRFADYTDVYAECRRAGLGLTVHAGETGGPDEVRRA
                     VEALEPDRIGHGVRASEDAGVMGLLRERGIVLEVCPSSNLNTGVLDDLAAVRRVVRSF
                     VDHGVRFTVSTDGPEMLRSMLRDELKLLLRHEILDLDEVRRAIAIGRDASFLDRAPVI
                     ESSVADRDGGGLGGGPDSGPESGSDGADSRAAIPVEAGA"
     gene            16303..17172
                     /locus_tag="VFV59_05670"
     CDS             16303..17172
                     /locus_tag="VFV59_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU5325354.1"
                     /translation="MSSLWRPVRERTPARSLMVVVAHPGDESFGFGGAIATAAAAGAY
                     VVVVCATRGWFDARLLDKSPAPGGKNRDVKYDAITWRNLDTVREDEMRRSVALLGVRV
                     LRMLDYAEGDLDRADFDLLVARIVEPIRMHRPEVILSFGPDGVTGDPDHVVLSRAVRA
                     AYERAAQPLAYEDDIEEDQVAWRAAKLYDLVVPARLTGEVLGDRTPLEGYGSAVAPTL
                     SLDLGEYAQLKLAAISRHVSQTGSVGPFHDWPAAARDAWLAQEHYRLVTSAVPVPAGA
                     EASPEGSLFDGLP"
     gene            17264..18370
                     /locus_tag="VFV59_05675"
     CDS             17264..18370
                     /locus_tag="VFV59_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEU5325355.1"
                     /translation="MANQLSDDNILAVLGGVKDPEIGRDLVSLGMVKNIAIDGSVVDL
                     TVELTTPACPLKAKIESDIVDALSGLGASLVRVDWASNVKRTFGGPAADLIPGVKNTI
                     AVASGKGGVGKSTVAVNLAVSLARDGARVGLLDADITGPNVPLMMGTQDAHVTGNGGR
                     PNPVVAHGVKMMSIQYFLQGDAPVIWRGPLIHSAIAQFLRDVEWGELDYLVIDLPPGT
                     GDAALSLSQLIPLTGAVIVTTPQEVSLLDARKAVAMFNKVNVRTLGVVENMSGFICDG
                     CGKEHDIFGSGGGAKIARELELEVLGRVPIEPGVRSGGDAGVPITSPQYPGGDDSRAA
                     QALREVARIIAGRVSVLAAPFAGVGSVTPTATAG"
     gene            complement(18345..19784)
                     /locus_tag="VFV59_05680"
     CDS             complement(18345..19784)
                     /locus_tag="VFV59_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEU5325356.1"
                     /translation="MDWSWFWATDASVARLVIERGIALVYLLAFVSAWHQFPALLGER
                     GLLPVPRYLAFLPFRRAPSLFHLGYTDARLRLVAGVGALGAAALLIGLPQQAPLPITM
                     ATWALLWVLYLSIVNVGQTFYAFGWESLLLDAGFLAIFLGNAEVAPPLLVILLFRWLT
                     FRLEFGAGLIKVRGDPCWRDGTCMRYHHETQPMPNPLSWWFHHLPEPLHRLEVYGNFV
                     TQLVLPFGLFLPQPIAGLSAAAMILTQGYLLVSGNYAWLNALTVILAFSGLPDAAFGP
                     LADGLGGGRADAPRWFIGLVLAWTLATVVLSWWPVRNLLGRGQLMNFSFNPFHLVNTY
                     GAFGSVTRERYEVIVEGTRSAVPDEDAEWLAYEFIGKPGDPRRRPPQVAPYHLRLDWL
                     MWFVPLSPAYASEWFPAFLQKLLDNDRPTLRLLRGNPFPDEPPRWVRARYARYRFTTM
                     SERRASGATWAVEPAGTLMPPVSRRSRSA"
BASE COUNT         2820 a         7123 c         7210 g         2691 t
ORIGIN      
        1 cggaagacgg cgcgctgggg gcggatgacg gcgcggaagt tgatgatctc ctgcttggcg
       61 ttgctgatct cgcgcacgat ctcgctcgag gctccgccct cgaagatgtc gtcctccagg
      121 cgtcgcagct tgcggcccag ggcaccgagc atggggaagc tggcgtcgac gcatcggtcg
      181 aggatctcgt acagcaggta gcccgagccc ttcgtcatca gctgctgccg cacgtcctcg
      241 cgcgccgccg ttcgctcgaa cagcgagggc agcgggagga tctcctcgtt ggggagggtg
      301 atgacgtagt tcggcccgac gaagacatcc agctccgctg cgtgcagccg accgctctcc
      361 ttgtcgtacc gggggaactg catgaccagg aacaggtagt cggcgtactc gtcgaccttc
      421 ggccgctggt tgtggctggc gacgtcctcg aggtcgagct cgtggaagcc gaactcctcc
      481 gccagccacg cgcggtcggc cagccggggc tcgtcgatgt tgatccagcg cagcgtcggc
      541 gcctcgatga tctccgggcg cggctcgcca cgcggctcct cgcccacgat ctcggtgccg
      601 gcgcccctgg gccgttggcg caggagccga gggaaggtct tcatcgggtc acccgccagg
      661 ccaaacgctc ggtatcgggc gccgcgctgc ggcccggcgc cggcgcacat tctagtgccc
      721 accggggcgt ctccggatgc tcgcgaagtc gccctctaga atgcgggacg tgccccgacc
      781 tcggagcgtc tcccggagcg gcgacgccgt catcagcaac gccgagctgg cgcgcatctt
      841 caccgagatc ggcgacatgc tcgagatcct cggcgaggtg gtctacaagg cggtcgccta
      901 ccggcgcgtc gccgacgcca tcgagcgcta cccggacgac gtcgcggcgc tcttccgacg
      961 cggcacgccg ccgaagctcc ccggcgcggg ttctgcgctg acggcgaagc tcgccgagct
     1021 gtccgagacc gggcggctgg agtaccacga gcggctccgg gcgcaggtgc cggccgggct
     1081 gctcgacatg ctgcgcgtgc cgggcatcgg gcccaagacc gtgcggctgc tccacgcgga
     1141 gctgggcatc gactccgtcg aggggcttcg ggctgcggcc gagcaggggc agctgcgaca
     1201 cgtgaaaggc ctctcggagc gcaccgagca gaacgtcctg gaggcgatcg ggcggatcga
     1261 gcgccgcagc acacgcctcc tcatccacga cgccgatgcg ctggtcgcga cgctgctcgc
     1321 cgacctggcc gacgttccgg gcgtgcgcag gatcgagcgg gccgggtcgc tgcgccgccg
     1381 gcgctcgacc atcggcgacc tggacctgct cgccgccgtg gacgatccgg tcgccgtgat
     1441 cgcgcgcctg gacggcctgc cggaggtcga gaaggtgctc tcggccggca cggacaagtc
     1501 cagcatcgtg ctgcgcgagg gcccccaggt cgacctgatg gtgtgcccgc cggacgcgtg
     1561 ggggacgcac ctcgtccact tcacggggag caaggaccac aacgtcgcgc tgcgcggcat
     1621 ggcgttggac cgcggctggt cgctgtccga gaaggggttc aaggtcatcg agacgggcga
     1681 gctgctgctc gaggccgacg aggaggccgt ctacgcgcgg ctgggaatgc cgtggatcgc
     1741 gccggagctg cgcgagggcg acggcgagat cgaggccgcg cttgccggcg cgctgcccga
     1801 gctggtgacg gtcgacgacg tccggggtga cacccacacc cactccgact ggaccgatgg
     1861 cgtcgactcg atcgagacga tggcgcgcgc cgcgcgcgac ctcggccacg agtacatggt
     1921 cctgaccgac cactcgccgt cgctcgggat cacgcgcggc ctgccgccgg ctcgtgtcga
     1981 ggagcagcgt gccgagatcc agcgcctgaa cggcgagctg gcaccgttcc gcatcctgca
     2041 cggaacggag atggagatcc gcgccgacgg atcgctcgac taccccgatg cgctgctggc
     2101 gcgctacgac gtcgtggtgg cgagcatcca caccggacgc gggcaaccgg ccgagcagct
     2161 gacgcgccgc gccctggcgg cgatcgagaa cccacacgtc gacatcctgg cccacccggc
     2221 cgggcggatc gtgaaccgcc gcgacccgct gccgctcgac tggccgcgcg tcttcgaggc
     2281 ggccgcccgc accgggacgg ccctcgagat caatggcagt ccgcgactcg acctcgagga
     2341 ctcgctggcg cgggccgcgg gacgtgcggg cgcgcgcctg accctcgcct ccgacgcaca
     2401 ccgcaccgag gagctgggac agcaacgcta cgcggtcgac gtggcgcgcc gcgcctggct
     2461 caccgccgac cagctgctcg cgacgcgctc cgccgatgac ctgctcgagc tgctgcggtg
     2521 atcacgaggc tcgtcaagga ccgtcgcagc ctcgcggtgc tgcgacgcat cgatcggccg
     2581 gcggcggtgc tgctggccat cgcgcaggcg gtgaccgcgg cggtcggctg gttcgtcgac
     2641 cccatctggc tggcggtcac ggtggcggtt cagctcgcgc tcggcggcat cggcgcggtg
     2701 cgggtcatcg gtgccggtcc cgccgagctc ggcttcgcgc gctacgcgat gccggcgacc
     2761 gccgggatcg ccgcgacgct cgtcgggcgg ctgctgccgg gtggcctcag cctgctgttg
     2821 gtgccgatcg tggcggtgct gctgtggtcg gtgtggtacc tcgagctgcg cctggccagg
     2881 ggcagcggcg gcctgacgat cggtggcctg cttcacacgg tcatcctctt cagtgcggcc
     2941 gccgggctgc tcgatctctt cgggcccaag gcgtggccga cgccgctgat cctggtcgcg
     3001 gtcttcgccc tgcccgtcgc cctgcgctcg gcggaggcgc gcggcgccgt gggtgccgag
     3061 ggcgtcggcc aggcgctgct gcacctgttg gtggtgatgg agatcggagc cggtgccgtg
     3121 ctgctcgacc tgccgatcac catcctggcc gcgctgatgg cactggcctt ctacgcctgg
     3181 gcgggtgccg tcgacgcgct gcgcggcggc acgtccgggc gagcggtcgc gatcgagtac
     3241 ggggcgttcg tgctgctcgg actgctggtg gcactgctcc tgcatcggtc ctgaccccgc
     3301 gccggggggc cgcgttgaca ccccccgggg gcggtggtac aagggggcgc tttcggcccc
     3361 tcgggccgat cctgacaccc tgttatcgga gtgctcgcaa cgctcatggc gcttggagaa
     3421 gccgacacca ccacacgaca acggcggcag ctcggggagg cggccatcgc gcaggccacc
     3481 cgcggggagt gggacgcggc cgtcgccacg aaccgccagc tgctcgagct cggaccggac
     3541 atcgacgcct acaaccggct cggaaaggcc ctggcggagc tgggccgcaa cgaggaagcg
     3601 gtcgaggcct acgagcaggc gctcgagcgc gacgccacga atcgcatcgc gcagcgcaac
     3661 gtcgagcggc tgcggctggt gatcgccgcc gcggccaaga cggacggcaa gggtcgggcc
     3721 gagaaggcgc ccgccacgct gttcatcgag gagatgggca agaccggtcg cgcgcggctg
     3781 ctgaacctgg ccggcccgcg cgtcctcgcc ccgctgtccc ccggggacgc ggtcgagctc
     3841 ggcgcggaga acgacgagct ggtcgcgcgc gtgggcggtg agctcatcgg cagtgtggaa
     3901 ccgcgcatcg gcagccggct gctgaagctc atggccggtg gcaaccgcta cgaggccgcc
     3961 atcaccacca tccacgaggc cggcggggag ctgtcgatca tcatccgcga ggtcttcacc
     4021 cacccgtcga acttcgggaa ggtgtcgttc ccgaccgtct cgcagggcgg cggtggcgtc
     4081 agaccgtaca tgaagggcag cgcacttcgg tacgatgacg acgaagaggt cgaggaggtc
     4141 gacgaggagt cggaggaggt cgaggagctc gacacgaccc tccccgagta ctcgggcgag
     4201 ccggacctcg aagaggagct cgtagaagag ccatagtcgg atgcgcgacg cggaacggac
     4261 gactctcgac cacgtcgtcc ttccgcgacg ccgtcccggg ctgccgctcc tcgaacgcct
     4321 ctttccgggg ccggccgaga acctgctcga cgccgagctg gccgccttcg ccacggaggg
     4381 cgacaccgtc ctggatccgt gggccggtgc cggctggacg gcacgacgga cggtccagcg
     4441 agggctccac gcgatcgcgg ccgacgcgag cccgctggcc caactggccg gcgtcacgct
     4501 gctgaccgcg cccgacccga tcgcgctcga cgccgccttc gcgcagctgg ccgggtcgcg
     4561 acgcgtggac gtcccgctgc ggcagcacct cgaggagctc tacgcgtcgc gctgccgcgc
     4621 ctgccgccgg ccggtggtcg tcgaccagtt catctggccc cgagacggtg acgcgccagg
     4681 gcggaagatc taccgatgcg ccgcctgcga ggcggcgctg ggtgggggag aggtggatcg
     4741 cgtggcgccc gtcgatgaga tcgacctcgc caagctcggg atcgacgcgg gtgccgatgc
     4801 ctcggtcgcg gaggagactg caccgttcgc cgccgcggcg ctcgacgccg cgggcgtgga
     4861 agatcccgat gctccgctgg gcgaggcagg cgggccgccg ctgcctccct ctccgcccgt
     4921 cgatccgccc gtcggagagg catcgcgtcc gcggttcgcg tcgaccgtgc gaccggaccc
     4981 cgtgccggtc gggtccgccg aggggatccg ccacgccccg cagtacctgg acctggtggc
     5041 gcgcttcccc gtgctggacg ggcgccatga cctggtggcg gagctgctgg acctctacac
     5101 accgcggaac ctgtatgcgc tccacgccat cggctcgaag atcgacagcg aggtgcacga
     5161 cagcggcgcg gccggcgcgc tgcgcctggc cctggcctcg gtcctcctgc cggcgagccg
     5221 cctgaacggg tatccgggcc gcgtcgccgg cctgcggatc agcggcggcc acgtgcgcca
     5281 gccggccagc cgtcaccagc gcgagatcaa cgtgtggcgc gccttcgagg aggcgtaccg
     5341 cgaggtgcgc gccgtcgttg ccgacgcggc gcgcgatcgc cgtccggcac ggttcgccgc
     5401 ggacgccggg gagctgggcg gcatggaggc ggcgaacgtg ctgtggctgc ggaccaggcc
     5461 cggcgtcgtg gcgcagtacc tgccggcgga gggcatcgac ttcgtcctca gcgcgccggc
     5521 gccgccccgc gcccacgacg agctgtcgtt cgagtacctc gccacctcgt ggatcctggg
     5581 acgcgaggcc gccgagacgc tgccgctcga gtccgtcttc gcgggcacgt cgccgccggc
     5641 cggtgccgag gcgaccgccg ttcgtcacgg cctggcatcg gccgcggccg cgctcaagcc
     5701 cggcggtcgc tgcagcctca tcctcgaggg cgccgacccg gatcggctcg tcaccttcgc
     5761 gcaggcggcg gccgcggtcg ggttcgagat cagcgacctc gtgtaccgcg agtcgcggcg
     5821 ctcgggcgac ggcctggtgc tccaccttcg caagccgtca gccgaggacc ggctgcgtgc
     5881 cgcggcacag cccgggccgc tgcgcctggg cgtggccgat ggccacctga cctatcccga
     5941 gctggcgtcc gccatccagc gcgccgtggt cgacctgctg cgagaccgcg gcgagcccgc
     6001 cggcctgttg cgcgtcgtca gcgccatcac gctggagctc gcgcgtgccg gcctcctgcg
     6061 ccgattcgcg accacgcgcg gcggcggcga ggccgatgcg gacgcagccg acgacgagcc
     6121 gaggaccctg gatgccgggc cgcatgccct ggcgaccctg atccgcgagg agctgtggcg
     6181 cgacgagcat cccgcactgg tgcggatcgg cgacgacagc gacccgcagt ggtggctgcg
     6241 cgacccggag ctggcccagg cgccgttggc ggaccgcgtg gagcgtgccg cgtggtcggt
     6301 gctgtccacg gctgggcgga tcgacgagga cgggttcctg gaccgcatct acgcgctctt
     6361 ccccggcctg gcggcaccgg acgaggagct ggttcgcgcc acgctggagg cctacgcggt
     6421 accgggcgag cgaggctcgc tgggcaccga cgacgacatc gcctcgcgga ccgaggacca
     6481 tgcccgcgtc atcgcgcggc tcgtcgagta cggccaccgg ctcgggctgc gcacctggat
     6541 cgcgaggcgc gagcaggagc gaacggtggc gggccgcccg ctgctcgccc tcctctccga
     6601 cgaggagcgc cgcgtctacc tccccctggt ggtgcgagga gcggccgatg cgctgggtgc
     6661 cgtcgacgtc atctggtacg tccgcgggaa gctggcgttc ctgttcgagg tcgagtggac
     6721 ggcagcggtc ggcgagacga tcctgggccg cgggcgggag gttccggtgg gcgagcagca
     6781 ggcgaggttc ctggtccttc ccgctcgtcg ggcggagctg ctgcggctca agctccagcg
     6841 ctcgccgtgg ctgcgcgccg aggtggagcg acagaactgg cacgtcctga agtgggagca
     6901 cctcgagacg ctggcggcgc gtccgggcgc acgcctggag tggctggagc cggtgctcgg
     6961 gctcgacccg ctcatcgagc gcgggggcga gcagctcacc atgttcggcg agtagcactc
     7021 aggggcgaca ggtgcttgcc cgctcggagg gcgaccgcta gactcgccgc gggagtgccg
     7081 tggttccccc ctgcggcact cccactcacg tcccacgatg cggctacgat gaggggatga
     7141 gccccctcga acgccacgag ctgatcagcc cgctcgagcg cgcgctcgtc gtgatcagcc
     7201 accccgacga cgccgagttc ggcgctgcgc ccaccatcgc catgctgacg cgcgccggcg
     7261 tgcgcgtgga ctacgtcgtg acgaccgatg gcagcaaggg cacggaggac cgggacgtcg
     7321 ccccccagca gctggccgcg acccggatgg ccgagcagcg cgacgcagcc gacatcctgg
     7381 gcgtcaccga catcgtccac ctcggctacc cggacggcta cctgacgccg tcactggacc
     7441 tgcgccgcga catcacccgc cagatccggc gctaccggcc cgacctggtg atcacgcaga
     7501 acccacagcg ccgcctggac cacaacccgt tcatcgggca cccggaccac ctggccacgg
     7561 gggaggcgac gctggcatcg gtctacccgg cggcgcgcga ccacctcaac ttcccggagc
     7621 tgtacagcga cgagcagctc gagccgtgga aggtgcgcca ggtcctgctc accggcgtcg
     7681 aggagccgaa cctgtggctc gacgtgtccg aaacgttcga cgtcggcatc cgctccatcc
     7741 tggctcacgt cagccaggtc gaccctggca ccgtcgagga gcgcatgcgg gagcgcgcgc
     7801 ggatcatcgg tgagccgcag ggcatcgggc tggcccaggc cttcctctcg atcatcctga
     7861 cgtgacgcgc cgcggcggag gcggcggtgg cccgtggggc cggttcgggg cgccggagcc
     7921 ggacctgacc cggcgaccgg gcagcgaacg gcagacgctg cgacgcatcg ccggcttctt
     7981 ccggccgtat cgcgggcgcc tgaccgtcat cgccgtcctg atcctgatta cggtcagcgt
     8041 cggcgtcgtc aacccgatcc tgatcaagct ggtcctggac aacctgctgc cgggagggcc
     8101 gcaggacctg gacctgctgt acctccaggt cgggctgatg gtggtgctgc cgatcatcac
     8161 cagcgccatc ggcgtctggc agtcctacct gtccaacgtg gtcggccagc gggtgatgga
     8221 ggacctgcgg ctggccctgt atcggcacct gcagtggatg cccctgcgct tcttcaccga
     8281 gacccgaacc ggcgagatcc agagccggat cagcaacgac gtcggcggcg tgcagagcgt
     8341 cgtcaccgat acggccgcct cgctcctgtc gaacttcgcg accgtcgcca ccaccgtcat
     8401 cgcgatgctc gtgctcgact ggcggctgac gctgctcagc ctgggcctcc tgccggtgtt
     8461 cgcctacatc acctaccgcg tcggcaaggt gcggcgcgag gtgtcgacct tgacgcagcg
     8521 ctcgctggcg gaggtcagcg ccatcaccga ggagtcgctg agcgtctcgg gcatcctgct
     8581 ctccaagacc ttcggccagc aggaggcgtc catcgagcgc ttccggcggg agtcccgacg
     8641 cctcggcgac ctgcagatcc gagggcagat gatcggtcgc tggttcttcg ccctgatcgg
     8701 caccttcttc agcatcatgc cggcgttcgt gtacctgctg gccggctcgc tgatcatcgg
     8761 cggggacacg aacatcagca tcggcacgat cgtggcgttc accacgctcc agtcgcgcct
     8821 cttcttcccg ctcgggcagc tgctgaacgt gcaggtcgag atccaggggg cgctcgccct
     8881 gttcgaccgc atcttcgagt acctcgagat ggagcacgac atcaccgatg cgccggacgc
     8941 ggtggccctc gcgccggcga cgatgcgcgg cgaggtccgg ttcgaccacg tcaccttccg
     9001 ctacccggtg gccgcggcgc agctccacgc ggccatcgag accgccgagg cggaggccga
     9061 ggggcgtgcg gtgccgaccc agatccagcc gttcgcgctc gaggacatct cgttcacggc
     9121 ccgccctggc cagctggtcg ccctcgtcgg gccgtcgggg agcggcaaga cgacgtccac
     9181 ctacctcatc ccgcggctgt acgacgtgga cgccggttcc gtatcgatcg acggcgtcga
     9241 cgtgcgcggc atccggctcg aatccctcgg acgtgtcatc ggcttcgtca cgcaggagac
     9301 gtacctcttc cacgacacgg tgctggcgaa cctgcgctac gccaagcccg aggcgacgat
     9361 ggaggagatc gaggaggcgg cgcgcgccgc cgcgatccac cagcggatca ccgagctgcc
     9421 ggacggctac gacacggtcg tcggcgagcg tggctacaag ctgtcgggcg gcgagaagca
     9481 gcgcgtggcg atcgcccggg tgctgctcaa ggacccgcgc atcctgatcc tggacgaggc
     9541 gacctcggcc ctcgacacgg tcagcgagcg gctgatccag gccgcgctgc agcggctgat
     9601 ggagggacgc accacgatcg ccatcgccca ccggctctcg accatcctgc gtgccgacca
     9661 gatcctggtc atgcaacgcg gccggatcgt cgagcgcggc acgcatgatg agctgctgcg
     9721 ccacgaaggg ctgtacgctc ggctgtcggc cgagcagttc gggccgggca gcgccgcctg
     9781 aggccggctc aacgtttcgc ccgtcccgtg cgtcggaccg gcatgacgat ggagatgggc
     9841 atgctcgaag ggctgcacgg atcgaccgcc caggccgatg cggatcggct ggccgtcgtg
     9901 gacctccagg cccgccgcgg ccccgagctc tacgggttcg cccgccgcct ggggctcggg
     9961 cccgaggccg ccgaggacgc cgtccaggag acgctgctgc gcgcgtggac cgtcctggcg
    10021 gatcggtccc cggtccgtga cctggacgcg tggtgcttca ccaccctgta ccgcctcagc
    10081 atggatcagc accgatggcg gcggcgcgtg cagcggctgg cggagcggct gcgccccgcg
    10141 ccaccgcggt cggagtccgc ctcggtcgcc gatcggctga ccctgtggga cgccgtcgac
    10201 cgcctgccgg agcggcagcg gaccgtggtg cagctccgct accgcgccga cctgaccttc
    10261 gaacagatcg ctcaggtcct gcacatcaag ccggtctccg cgcgcagcca tgcgagccgc
    10321 gccctggacg cgctggccag cctgctggcc gaggaggact tccgatgacc cgcgacgagc
    10381 tcgagcgagc cctgcgttcg tcggtcgacc cccacgagcg gacctaccgt gccgcgccgc
    10441 tgccggccag cgtggaggag gcccggagcc ggatcggggt tcgtcggacc aacggcggga
    10501 gcgcgctccg cttcgccggc gtcgtggtcg ccacggcgct ggcggtggtg gccgtggcgg
    10561 ccgggtacgg cctggtgacc ggcgggcgcg gcaccggcgg cgatgcctcg gccagcgacg
    10621 cttcgatggt gccgatcacc acgcccgctg cgtccggctc cgtggcagcc cgtccgtgcc
    10681 gggcggccga cttcgcgatc accagcgatc cctgggatgc cggggcgggg tcgcggggga
    10741 cgcgcgtcgt gttccgcgcc gccgactcgc tcgatgcctg cgtcctgggc ggccggctcg
    10801 ccgcccggat cgtcgacgcg caggacgagg tgctggtcgc ggccgagtcc gccgagacgc
    10861 cgaccacgac cgtgaccggt ggcgtccagc tggagatcgg cgtgaggtgg agcaactggt
    10921 gtggcgccgc gccccgggag ccgctcgagc tgcgcgtgcg cttcccgtcc gactcggcgg
    10981 aggttgcgct cgtcccgccg gccgggacct cgatcctcgt gcccccgtgc atgggcggcg
    11041 gggagggcac ggcgctgagc ctgaccgagt tccaggcgtc cgaccgaccg gctcccgagg
    11101 gctgagccgc ggacgaagcc tcagcccagg ggcacgaggc cgacgtcgcc ctcgaggcgc
    11161 cagccctcct cgcccggctc gtcggcggtc ccccaggcct cgtagaagcc ctgcgccgct
    11221 gaccggctgg tccagtgcgc cagtgcccag aaggccgtcg tcctgcatgc cacgcatcgt
    11281 acggcgcggc acaacgcgtg cgagactggc gtccatgccg aaccgcctcg ccgaccagac
    11341 gagcccctac ctgctgcagc acgcgcacaa cccggtcgac tggtacccgt gggggccgga
    11401 ggccctcgag cgggcgcgcg cggaggatcg gccgatcttc ctctccatcg gctacgccgc
    11461 ctgccactgg tgccacgtga tggagcgcga gagcttcgag aactccgaga tcgccgcgct
    11521 catgaaccgg gagttcgtgg cgatcaaggt cgaccgcgag gagcgcccag acctcgacga
    11581 cgtctacatg gccgcggtgc aggccatgac cggctccggc ggatggccga tgagcgtctt
    11641 cctgaccccg gacctgcgtc ccttctacgg cggcacgtac ttcccgcccg aggatcgcca
    11701 cggcatgccc ggcttcccgc gcgtcctcga ggccgtcgcg gacgcctacc gcaaccgtcg
    11761 aagcgagatc gagcagcagg ccgggatgct ggcgtcccac ctggcgtccc agctggccgt
    11821 cgcccccggc cgctccgaac cgcagtggca gcagcttgac gccgccacga cgcgcctggc
    11881 cgccagcttc gacgacgtgc acggcggctt cggcagtgcg ccgaagttcc cgccgcccat
    11941 gaccctcgag ttcctgctgc gctcgtggca ccgaacgggc agcgcgcggg cgctgcgcat
    12001 ggtgacgcgg acgctcgacg ccatggccga cggcggcatc cacgaccagc tcggcggcgg
    12061 cttcgcccgc tacagcaccg acgcgcactg gctggtgccg cacttcgaga agatgctgta
    12121 cgacaacgcg ctgctggcgc acgcctacct ggaggcccac cgggcgaccg gcacggaccg
    12181 atacgcgcgc gtggcgcgcg acaccctcga cttcatgctg cgcgagctcc gcacggccga
    12241 tggcggcttc gcctcggcgc tcgacgccga cagcgagggc gttgagggtc gcttctacgc
    12301 ctggaccgat gcggagctcc gctccgccct ggccgacgcc ggcttcggcg ccgacgaacg
    12361 ggccgccctg gccgcgtact ggggcgtcga gccggctggc aactgggagg ggaccaacgt
    12421 cctccacgtc agcgaccccg gcgccgccga cgcgacgctg gtggaacgag ctcgccaggc
    12481 gctcctggag gctcgcgagc gccgcacccg gccggcacgc gacgacaagc agctggcctc
    12541 gtggaacggg ctcgccctgc gcgccttcgc gcgtgccgcc ctggtcctgg acggtccctc
    12601 gtacctggcg gtcaccgccg agctcgtcga gttcgtcgag cgccacctgg tccgcgacgg
    12661 gggacgccgg ctgtggcgca cggtgcggga cggccaggcc cacaccccgg ccttcgcgga
    12721 ggactacctg gccgtggccg acggcctgct cgacgcccac gcggcgctgg ggacggcggc
    12781 gccgctgctg ctcgcgcgca cgctggtcag caccgccgtc gccgagttct gggacgacga
    12841 ggccggaacc ttcgtcgaca catcgtccga gcacgaacgg acggtcgcac agcctcgcgg
    12901 catggtggac aacgccgtcc cgtcggccaa cggcctcggc gcggacgtgc tccagcggct
    12961 cgcgctgctg accggcgacg aggatctcgc tcgtcgagct cgcgcgatcc tctcggcggt
    13021 cgcaccggcc ctcgaccgcc agccgagcgc cttcgggcgc atgctggggg ccgcggatcg
    13081 gctgctggga gagccgatcg acgtcgtcgt ggcctggacg gacgccgacc gcggacagcc
    13141 cctgcgcgtc gcggccatgc gtccctacgt gcccgacctc gtcctgacgt cggtgggcgg
    13201 cggcgacgag cacgcgagct ggcccctgta cgccgccaag gtcgcccgag ccgaccgcgc
    13261 gacggcgtac gcgtgtcgcg ggtacgcgtg cgacgagccg acggacgacg cgggccgcct
    13321 ggtcgagcag gtccgcgggc tggcgagggc ggcagccgag gtcagccggc caggaagctg
    13381 agctgcaccg tgcgccgggg gttgtcgcgg ttggggtcga ccagcgcgac cgactgccag
    13441 gtcccgagcg tcatccggcc accaacgacg ggcacgctca ccgacggcgc gaccagcagc
    13501 ggcaggacgt ggtccgcccc gtggcccggc gagccgtgcc ggtgtcgcca gcgctcgtca
    13561 cgcggcagca gccgctcgag ggaggccagc aggtcgtcgt ccgacccggc gccgagctcc
    13621 atgatcacca cgccggcagt ggcgtgcggg acgaagacgg tgagaagccc gtcccgcgcg
    13681 tggacgtccg ccaggaatcg gacgcacgca tcggtcaggt ccaccacggc gggctggctg
    13741 ccgctctcga gcgtgatctc gcggtgctgc atcacggcct cccgtagctg tgctcgccga
    13801 cgatgagaag gccggctccc aggtaggtga agaggacgcc cgcgaacccg acgacgatca
    13861 gccatggcgc cagccggccc cagccatcgg tgcgggtcgc gacgtgcagg taggcgccga
    13921 agaccagcca cgtggccgcg gcggcaagct ccttgggatc gttgctccag tacgagcgcc
    13981 acgccaggtt cgcccagacc gagcccagca cgatggccac ggtcaggatg gggaagccga
    14041 tgagcaccgc gcggtgtccg acggtccgca gcaccgcgac ggacggcagc aacgcgatcc
    14101 ggcccccggc cgcgcgttgc ccgagctcgg ccgccgccgc gaggaaggcg acgccggcca
    14161 gcgcgtaggc gaggatcgcc gtgccgacgt ggacggtgag cagcaacggt tgctggagcg
    14221 ccggaacgag cggctggacg cggtcatcca ggttcagcgc gacggtgatc agcccggccc
    14281 ccagcaccgc ggccagcggc gccaggccgg agatcggaac gctgcgcgcg agggcgagat
    14341 aggcgaggag cagcatggcc gcgaaggcga tgctcacctc gtggaggttc gaccacggtg
    14401 cgtgcccggc ggcgaccgcc cgcgcgacgg acgccgcgac cagcagcccg gccccgagtc
    14461 cgacgccagg gacgagcagc ccggcgagcg gcggtcgggg agcgtcgacg tccgcaccgc
    14521 cggtggtcgc gagcgggcgt ggggcgagga cggccacgac gaaccccgcg gcggccgtgg
    14581 cgagcagcac cgcgagggcg gcgacgagga gtggagtgat gagctcggtc acgcgatgtc
    14641 ccggtcgagc ggccggatcg gccgcggccg gtcaggcgag gtggtgggcg gccgcatcca
    14701 cgccggtcgg cgtgccgcag cggtagcagg acgcatcgcc cgccgagagg gttgcgccgc
    14761 aggtggcgca gtcgagcacc aggtcgcgct cgcaggcggt gcagaagcgg gccccaagcg
    14821 ggtaggcgag gccgtcgaag gcgcagtacg gcacgccctg cggggctgcg gggagcgcgt
    14881 tcgggtcggt ggccggcggc agcgcgaccg cctgctgcac ggcctgcccg tcgtggatgc
    14941 cgacctggct gacgcggagg tagccgatca ggagccacag caggaagagg ccgacgacgc
    15001 cgagcatcag gaatgggaag aggtcgaaca gcagcttcgg cgagtcggcg gcgatccact
    15061 cgatcgcgtt gcggatgaac tccatggccg ccgattctag cagggccgat cacgcgcgaa
    15121 gcgtgcgcta cactcgccgg cacactcgcc accgacccgc cgatgcccgc ctcgcgactc
    15181 gatcccagaa cgccgctgac cgagctccac gtccacctcg gcgcggccgt cacgccaccg
    15241 atcatgtggg gcatcgcgca cgcccagggc atccgcctgc cgaccaagga ctactgggcg
    15301 tttcgtgacc tcatcaccgt cggacgccgc aagcgctcgt tccaggccta cctggacctc
    15361 ttccactgga ccgagctgat ccagtccagc ccgctggccg tcgagcgcag cgtgtacgag
    15421 gtcatcggcg gcgcgtaccg caagaacaac atcacgcagc tcgagctgcg gttcaatccg
    15481 atgaagcgca accgcggcgg cgaacaggac ctggaccaca tcatcgcggc ggccgtgcgc
    15541 ggcatggacc gcgcggtact cgagtacccg caggtccgtg ccgggctcgt cttctgcctg
    15601 gatcgcggct tcagtcgcga gctgaacgag atcatcgcgg ccaaggccat cgcctggcac
    15661 agccgaggcg tggtcggcat cgacatcgcc gggccggtgg tcgatcggtt ccgcttcgcc
    15721 gactacaccg acgtctacgc cgagtgccgc cgcgccggcc tgggcctgac cgtccacgcc
    15781 ggcgagacgg gcggccccga tgaggtgcgc agggcggtgg aggcattgga gccggaccgc
    15841 atcggtcatg gcgtgcgggc gtcggaagat gccggcgtca tgggcctgct gcgtgagcgc
    15901 ggaatcgtgc tggaggtgtg tccctcgtcg aacctgaaca ccggcgtgct cgacgacctg
    15961 gccgccgtcc ggcgggtcgt ccggtccttc gtcgaccacg gcgtgcggtt cacggtcagc
    16021 accgacgggc cggagatgct gcgctccatg ctgcgcgacg agctcaagct gctgctgcgg
    16081 catgagatcc tggacctgga cgaggttcgg cgcgccatcg ccatcggccg tgacgcgagc
    16141 ttcctggacc gcgcgccggt gatcgagagc tcggtcgccg atcgggacgg cggcggcctc
    16201 ggtggcggcc ccgacagcgg ccccgagagc ggctccgacg gcgccgattc ccgtgcggcc
    16261 attcccgtcg aggccggcgc ctaggcccgt atactgccgg ccgtgtcctc cctctggcgg
    16321 ccggtccgcg agcgcacccc cgcgcgcagc ctgatggtcg tcgtcgccca cccgggcgac
    16381 gagtcgttcg gcttcggcgg cgccatcgcc accgcggcgg cggccggggc atacgtggtc
    16441 gtggtgtgcg ccacgcgcgg ctggttcgac gcacgcctgc tggacaagtc gccggcgccc
    16501 ggcgggaaga accgggacgt caagtacgac gccatcacct ggcgcaacct cgacacggtg
    16561 cgcgaggacg agatgcgccg ctccgtcgcc ctcctgggcg tccgcgtgct ccgcatgctc
    16621 gactacgccg agggcgacct cgatcgggcg gacttcgacc tgctggtggc gcggatcgtg
    16681 gagccgatcc gcatgcatcg gccggaggtc atcctctcct tcgggcccga cggcgtcacc
    16741 ggagatccgg accacgtggt gttgtcccgc gccgtccgcg cggcctacga gcgggcggcc
    16801 cagccgctgg cctacgagga cgacatcgag gaggaccagg tcgcctggcg ggccgcgaag
    16861 ctctacgacc tcgtggtgcc ggcccggctc accggcgagg tgctcggcga tcggacgccg
    16921 ctcgaggggt acgggtcagc ggtcgcgccg acgctgtcgc tcgacctcgg cgagtacgcg
    16981 cagctgaagc ttgccgccat ctcccggcac gtctcgcaga ccggctcggt cggtcccttc
    17041 cacgactggc cggctgcggc gcgcgacgcg tggctcgccc aggagcacta ccgcctggtg
    17101 acctccgccg tcccggtgcc cgccggcgcc gaggcctcgc cggagggctc gctgttcgac
    17161 gggctgccct gacccgaccg gttcgggaac tgaagcgtcg gggcacgtct ggtacgctgc
    17221 aggggccgca tcggcccctc tgctaggagt tcccgagcca gtcatggcca accagctcag
    17281 tgacgacaac atcctcgccg tgctcggcgg ggtgaaggat cccgagatcg ggcgcgatct
    17341 ggtgtcgctc gggatggtga agaacatcgc gatcgacggc agcgtcgtgg acctgacggt
    17401 cgagctcacg acgcccgcgt gcccgctgaa ggcgaagatc gagagcgaca tcgtcgacgc
    17461 gctgtccggc ctcggcgcca gcctcgtgcg cgtcgactgg gccagcaatg tgaagcgcac
    17521 cttcggtggc cctgcagccg atctcatccc cggcgtgaag aacacgatcg ccgtggcgtc
    17581 aggcaagggc ggcgtcggca agtcgacggt cgccgtgaac ctcgccgtca gcctggcgcg
    17641 cgacggcgcg cgggtcggcc tgctggacgc cgacatcacc gggcccaacg tcccgctgat
    17701 gatgggcacg caggacgccc acgtcaccgg caacggcggg cggccgaacc ccgtcgtcgc
    17761 gcacggcgtg aagatgatga gcatccagta cttcctgcag ggcgacgcac cggtcatctg
    17821 gcgcggcccc ctcatccaca gcgccatcgc ccagttcctg cgtgacgtcg agtggggcga
    17881 gctcgactac ctggtcatcg acctcccacc ggggaccggc gatgcggcgc tcagcctcag
    17941 ccagctcatc ccgctcaccg gcgcggtgat cgtcaccacg ccacaggagg tcagcctcct
    18001 cgacgcgcgc aaggccgtgg cgatgttcaa caaggtcaac gtccgcacgc tgggcgtggt
    18061 cgagaacatg agcggcttca tctgcgacgg gtgcggcaag gagcacgaca tcttcggatc
    18121 cggcggcggc gcgaagatcg cccgtgagct cgagctcgag gtcctcggcc gcgtgccgat
    18181 cgagcccggc gtccgatccg gcggcgacgc cggtgtgccg atcacctccc cgcagtaccc
    18241 cggcggcgat gactcgcgcg ccgcgcaggc gctgcgcgag gtggcgcgca tcatcgccgg
    18301 gcgggtgagc gttctggcgg cgcccttcgc cggcgtcggt tccgtcacgc cgaccgcgac
    18361 cgccggctga ccggcggcat cagcgttccg gccggctcga cggcccaggt ggcccccgag
    18421 gcgcggcgct cgctcatcgt cgtgaagcgg tagcgcgcgt atcgcgcgcg gacccagcgc
    18481 ggtggctcgt cggggaacgg gttgccgcgc agcagccgca acgtcggccg gtcgttgtcc
    18541 agcagcttct gcaggaacgc cgggaaccac tccgacgcgt aggccggcga caacggcacg
    18601 aaccacatca gccagtccag ccgcaggtgg tacggcgcga cctgcggcgg ccgtcgccgc
    18661 ggatcgccgg gcttgccgat gaactcgtac gccagccact cggcgtcctc gtccggcacg
    18721 gcggatcgcg tgccctcgac gatgacctcg tagcgctcgc gcgtcacgct gccgaaggcg
    18781 ccgtacgtgt tcaccaggtg gaacgggttg aacgagaagt tcatgagctg gccgcggccc
    18841 aggaggttgc ggacgggcca ccagctcagc acgacggtgg ccagcgtcca ggccagcaca
    18901 aggccgatga accagcgggg tgcatccgcg cggccgccgc ccagcccatc ggccagcggc
    18961 ccgaaggcag cgtccggcag gcccgagaag gccaggatga cggtcagcgc gttgagccac
    19021 gcgtagttgc cgctgaccag caggtagccc tgggtcagga tcatggcggc ggcgctgagc
    19081 ccggcgatcg gctgcggcag gaagagcccg aacggcaaaa ccagctgcgt gacgaagttc
    19141 ccgtacacct cgagccggtg cagcggctcc ggcaggtggt ggaaccacca cgagagcggg
    19201 ttcggcatcg gctgcgtctc gtggtggtag cgcatgcagg tgccgtctcg ccagcacggg
    19261 tcgccgcgca ccttgatcag gcccgctccg aactccaggc ggaaggtcag ccagcggaag
    19321 agcaggatca cgaggagtgg gggcgccacc tccgcgttgc ccaggaagat ggccaggaac
    19381 ccggcatcca gcagaagcga ctcccagccg aaggcgtaga aggtctggcc gacgttgacg
    19441 atcgacaggt acaggaccca cagcagcgcc caggtcgcca tcgtgatggg cagcggggcc
    19501 tgctgtggga gcccgatgag cagcgccgcg gcgcccagcg cgccgacgcc ggcgacgagg
    19561 cggaggcgcg catcggtgta gccgaggtgg aagaggctcg gcgcgcgccg gaacggcagg
    19621 aaggccaggt agcggggaac cgggagcagg ccgcgctcac cgagcagggc ggggaactgg
    19681 tgccaggcgg acacgaaggc cagcaggtag accagggcga tgccgcgctc gatgacgagg
    19741 cgcgccacgc tcgcatcggt cgcccagaac cagctccagt ccacgtcgct ccacgcctgc
    19801 gaggtccgtg ccggatgaga cgatggtcac ccggaaggcc catt
//
LOCUS       DASTYP010000123         6039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_591790, whole genome shotgun sequence.
ACCESSION   DASTYP010000123 DASTYP010000000
VERSION     DASTYP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591790"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFV59_05685"
     CDS             complement(<1..880)
                     /locus_tag="VFV59_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5325357.1"
                     /translation="MIDFSLTDAQREVQRTARAFAEREILPRISEMDANATYDRSLYE
                     KMGSEGFLGLPIPEEYGGAGMDYIAFALLCEEMERADTAFRVILSVHTGLNSLTLLQW
                     GNEEQKQKYLVPQARGEKLATFGLTEPGVGSDAGQLQTTARRDGDRYILNGSKIWISL
                     ADTADHFLVFANVDRSKGYKGITAFIVERSFPGFSSESLHGKLGVRAGNTGILTFSDC
                     EVPAANRVGEEGEGFTIAMSAIDQGRYTVAAGSVGLAQACLDASVKYAHERQAFGEEI
                     GRHQLVKQMIAKMHQGI"
     gene            1103..1972
                     /gene="efeU"
                     /locus_tag="VFV59_05690"
     CDS             1103..1972
                     /gene="efeU"
                     /locus_tag="VFV59_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEU5325358.1"
                     /translation="MSTGLLTGLREGVEAALIVSIILAYLAKTGNQRHFSAIWLGVGA
                     AIVVSIGIGVALFVTIGGFEEPAEQIFEGFAMLIAAVVVTWMLFWMRRTSANIKGELQ
                     AGVDRALVSGGLLGLGALAFTAVIREGIETALFLMGQATAAGSQASSTLVGAVLGLVL
                     AVVIGWALYRGARIINLHTFFTWTGVALIFIAAGLLSYAVHEFIEAGWITVGTATAFD
                     ISGILPHSPDAGPMGVVGSILRALVGYTSTPEWATFIAWLVYIVVVLVLYTRPVRPSA
                     SRASSPERTAASA"
     gene            complement(2009..2782)
                     /locus_tag="VFV59_05695"
     CDS             complement(2009..2782)
                     /locus_tag="VFV59_05695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5325359.1"
                     /translation="MCFDADARPPLPPIRGGAIDARDLTLTSRDGTRFAAHAARAEQP
                     AGAGIVIIPDVRGLAPYYEELAHRFAEAGVHAVAIDFYARTAGAEKRGADFEYEPHVS
                     QVTIDGAAADVAAAVAFLRSPEGGGATALYTVGFCLGGRLSLLQATSGLDLAGVIGFY
                     PWPFGPHRSGLPAPTDEAPRFGCPVLALYGGEDRGIPAEARDAFDRALDAAGIEHRSV
                     VYDGAPHSFFDRKAGDFADASADAWAEVLAFMNVSGAAR"
     gene            complement(2823..3986)
                     /locus_tag="VFV59_05700"
     CDS             complement(2823..3986)
                     /locus_tag="VFV59_05700"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5325360.1"
                     /translation="MPDEPTLRLEEGRRLLDTGDFAAAVRILAPLTGHPDPDLAGEAW
                     LAIGTARYRADDEPGALAAWQQAANTNGASAWLGWRSVAEQLVRDGDLDSAIGAYREA
                     DRRAPPGERGAIANRIAWLLKETGHDFEARRQFNRARGAYATHVPVVTWAIIAICVAV
                     FGIDALLSGGSILSGGLFGSVGPVGEGQLLNGIMVASGEWWRILTSTFVHLGLIHLGF
                     NMYVLYLYGPVVERMYGSLEYALIYLLCGAGGSVLTILWDPTQFAAGASGAIFGIIGL
                     LFAVSRRHHAVLGREARSMMAGIGSYLVFLLIFTFVMPRISWTGHLGGLVVGFVLGWL
                     LPPTGVATLGSLWRSPTGEQLHRGMPVSLRLAVYAGMLILLVVGSVVAANLVG"
     gene            complement(4140..4322)
                     /locus_tag="VFV59_05705"
     CDS             complement(4140..4322)
                     /locus_tag="VFV59_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5325361.1"
                     /translation="MTNEVGRLLTASEVAEMLHLHVNTVKRLGDRGELPFFRVCKRGD
                     RRFRYDDVIDFLRRSK"
     gene            4473..5387
                     /locus_tag="VFV59_05710"
     CDS             4473..5387
                     /locus_tag="VFV59_05710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5325362.1"
                     /translation="MTDASPPPDVRALLEERAAAREARDWATADALRDRIAALGWEVQ
                     DSGGATTARPMLATGTVEELPSLLDAPAELPLGLQLLAEDHPDDLRRFLRGLAAHPPS
                     VPWELVIVANEPSYDLPALLAAFELPIAVLESHGRLGWGDAVTLGLRRSRGEITVLLD
                     TSLEPTGDAWTQLLAAFDDPTVGVAGGWGVTSGDARQFEDAPPGEVDAVEGYCLAIRR
                     AALRAVGGVDRRFRFYRNADLDLSFAVRDAGWRAMRTEPLPFDRHEHRGWTSLPDAER
                     DRLSKRNFYRFLERWGSRRDLLLHPDQS"
     gene            5384..5689
                     /locus_tag="VFV59_05715"
     CDS             5384..5689
                     /locus_tag="VFV59_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325363.1"
                     /translation="MKAPTVHVVPWLRPLGARVIPNWLAITIGRHVFAWRALTDVELE
                     HELEHARQWARIGWAFPLAYLADSMRVRRRGGRWYDDNRFEREARAAAAARRTNPKV"
     gene            complement(5686..>6039)
                     /locus_tag="VFV59_05720"
     CDS             complement(5686..>6039)
                     /locus_tag="VFV59_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5325364.1"
                     /translation="LRAPGRLMLPPPLRPLPGPNAPGRVAAEERLRAAGVTPYAWGNG
                     PGDTYAAHEHGYEKLLVCADGSITFLVGSDQTPVTLSAGDGLVLPAGTRHAAVVGPEG
                     CTCLEGHRAGWAESP"
BASE COUNT          862 a         2173 c         2125 g          879 t
ORIGIN      
        1 cgatgccctg gtgcatcttg gcgatcatct gcttgaccag ctggtgccgg ccgatctcct
       61 caccgaaggc ctggcgctcg tgcgcgtact tgaccgatgc gtccaggcac gcctgcgcca
      121 gcccgacgga tccggcggcg acggtgtacc ggccctggtc gatcgcgctc atcgcgatcg
      181 tgaagccctc cccctcctcg ccgacccggt tcgcggccgg cacctcgcag tcactgaacg
      241 tcaggatgcc ggtgttgccg gcgcggacgc cgagcttgcc gtgcagagac tcggagctga
      301 agcccgggaa ggagcgctcg acgatgaacg cggtgatccc cttgtatccc ttcgaccgat
      361 cgacgtttgc gaagaccagg aaatggtcgg ccgtatcggc caggctgatc cagatcttgc
      421 tgccgttgag gatgtagcgg tcgccgtcac gccgcgcggt ggtctgcagc tggccggcgt
      481 cggagccgac ccccggctcg gtcaggccga aggtcgccag cttctcgcca cgcgcctgcg
      541 ggaccaggta cttctgcttc tgctcctcgt tcccccactg cagcagggtc agcgagttga
      601 gaccggtatg aacggacagg atcacgcgga aggcggtgtc ggcgcgttcc atctcctcgc
      661 atagcagcgc gaaggcgatg tagtccatgc cagcaccgcc gtactcctcg gggatgggca
      721 ggccaaggaa accttccgaa cccatcttct cgtagagaga gcggtcgtag gtggcgttgg
      781 catccatctc ggagatgcgg ggcaggatct cgcgctccgc gaaggcgcgc gccgtgcgct
      841 gcacctcgcg ctgcgcatcg gtcagcgaaa aatcgatcat ggggaatggt gctccctgcg
      901 ggaactcggg gtcactcggg tgggacggcc cgattctaga ccgatgctgc ggtggagcag
      961 cgccacctgg aatcccgacc agttcatcgg tgattcggtt tgacgaagga acggagtaag
     1021 gctagcctaa ctcgccgtcg ggccggacga tccagccagg gtcgaactcg acgcacggga
     1081 gagcacgttg gacgtcgggt tcttgagcac cgggctcctg accgggttgc gggagggggt
     1141 cgaagccgcc ctcatcgtca gcatcatcct cgcgtacctg gccaagacgg ggaaccaacg
     1201 tcacttcagc gcgatctggc tcggcgtcgg cgccgcgatc gtcgtcagca tcggcatcgg
     1261 cgtcgccctc ttcgtcacca tcggtgggtt cgaagagccg gcggagcaga tcttcgaggg
     1321 cttcgccatg ctgatcgcgg ccgtcgtcgt gacgtggatg ctcttctgga tgcgacgcac
     1381 cagcgccaac atcaagggtg agctgcaggc cggcgtggac cgggccctcg tgagcggcgg
     1441 gttgctcggg ctgggcgccc tggccttcac cgccgtcatc cgggagggga tcgagaccgc
     1501 gctcttcctg atgggtcagg cgacggccgc ggggtcgcag gccagcagca cgctcgtcgg
     1561 ggcggtcctg ggcctggtgc tcgccgtcgt catcggctgg gcgctctacc gcggcgcgcg
     1621 aatcatcaac ctgcacacct tcttcacctg gaccggcgtc gcgctcatct tcatcgccgc
     1681 cgggctgctc tcctacgccg tccacgagtt catcgaggcc ggctggatca ccgtggggac
     1741 cgccaccgcc ttcgacatca gcggcatcct gccgcacagc ccggatgccg gaccgatggg
     1801 cgtcgtcggc tccatcctgc gcgcgctggt cggctacacg agcacgcccg agtgggccac
     1861 gttcatcgcc tggctggtgt acatcgtcgt cgtgctcgtg ctgtacaccc gaccggtccg
     1921 tccgtccgcg tcgcgcgcct cctcgccgga gcggaccgca gccagcgcgt agcggccacc
     1981 ctcaccgcga ggccggcgtc gtccgccgtc agcgtgcggc gccactcacg ttcatgaagg
     2041 cgagcacctc cgcccatgcg tccgcgctgg cgtcggcgaa gtcgcccgcc ttgcgatcga
     2101 agaacgagtg cggggcgccg tcgtacacca cgctgcggtg ctcgatcccg gcagcgtcga
     2161 gcgcgcgatc gaacgcgtcc cgggcttcgg ccggaatccc ccggtcctca ccgccgtaca
     2221 gcgcgagcac cgggcacccg aatcgcggcg cctcgtccgt cggggccggc agcccgctgc
     2281 gatgcgggcc gaagggccac gggtagaagc cgatcacccc ggccaggtcc aggcccgacg
     2341 tcgcctgcag caggctgagg cggccgccca ggcagaagcc gacggtgtag agcgccgtcg
     2401 ccccgccgcc ctccgggctg cgcaggaagg cgacagcggc ggcgacgtcc gccgccgcac
     2461 cgtcgatggt cacctgggac acgtgcggct cgtactcgaa gtcggccccg cgcttctcgg
     2521 cgcccgcggt gcgcgcatag aaatcgatgg ccaccgcgtg cacgccagcc tccgcgaagc
     2581 ggtgggccag ctcctcgtag tacggcgcca gcccgcgcac gtcggggatg atgacgattc
     2641 ccgcaccggc gggctgctcg gcgcgggcgg cgtgcgccgc gaagcgcgtg ccgtcccggc
     2701 tggtgagcgt caggtcgcgg gcgtcgatcg caccgccgcg gatgggcggg aggggtggtc
     2761 gggcatcagc gtcgaagcac atgccgtgca ggatcgcacg cgccgtgccg cccgtgtggc
     2821 gatcagccga cgaggttcgc ggcgaccacc gagccgacga ccaggaggat cagcatcccg
     2881 gcgtagacgg ccaggcgcag gctcaccggc atgccgcggt gcagctgctc gcccgtcggc
     2941 gagcgccaca ggcttcccag cgtcgcgacc ccggtgggtg gcagcagcca gcccagcacg
     3001 aagcccacga ccaggccgcc caggtggccg gtccagctga tgcgcggcat gacgaaggtg
     3061 aagatcagca ggaagaccag gtagctgccg atgccggcca tcatgctgcg cgcctcgcgc
     3121 ccgagcaccg catggtgacg gcgggagacg gcgaacagca ggccgatgat gccgaagatg
     3181 gcgcccgacg caccggccgc gaactgggtc gggtcccaca ggatggtcag cacgctgccg
     3241 cccgcgccgc acagcaggta gatcagcgcg tactccagcg agccgtacat gcgctcgacc
     3301 accggcccgt acaggtacag cacgtacatg ttgaagccca ggtggatcag cccgaggtgc
     3361 acgaaggtgc tggtcaggat ccgccaccac tcgccgctgg ccaccatgat cccgttgagc
     3421 agctgtccct ctcccacggg gccgacgctg ccgaagagcc cgccggacag gatgctgccg
     3481 ccggagagca gcgcgtcgat gccgaacacc gcgacgcaga tggcgatgat cgcccaggtg
     3541 accacgggaa cgtgcgtggc gtaggcaccc cgggcgcgat tgaactgccg gcgcgcctcg
     3601 aagtcgtggc cggtctcctt cagcagccac gcgatccggt tggcgatcgc gccccgctcg
     3661 ccgggcgggg cccgccggtc ggcctcgcgg taggcgccga tcgcggagtc caggtcgcca
     3721 tcgcgcacga gctgctcggc cacgctgcgc catccgagcc acgcgctggc gccgttcgtg
     3781 ttggccgcct gctgccaggc cgccagcgca cccggctcgt cgtccgcgcg gtagcgggcg
     3841 gtgccgatgg ccagccaggc ctcccccgcg aggtcgggat cgggatggcc ggtcagcgga
     3901 gccaggatgc ggacggcggc ggcgaagtcg ccggtgtcca ggagccggcg cccctcctcg
     3961 aggcgcagcg tcggttcgtc gggcatgcgc ggccgattat gcccggatcg tcacgcacac
     4021 agcgaacgac cgccgagaga ttggcacctc ggcggtcgtt cgtgccaccg cccccctggc
     4081 agcggcctgc cccggtgtgg gcccgggagc atcgaatgcg gtcggagtcg ggcgggcggc
     4141 tacttgctcc ggcggaggaa gtcgatcacg tcgtcgtagc ggaagcgccg atcaccacgc
     4201 ttgcacacgc ggaagaacgg cagctcgccc cgatcgccga ggcgcttgac cgtgttcacg
     4261 tgcaggtgga gcatctcggc gacctcggac gccgtcagca gccttccgac ctcgttcgtc
     4321 atcatgggtt gcgtctggtc ctcgtgtagg gcactgaccg cgcgcggtgc accgggccgg
     4381 tgcagtcggt gcggctcgtg gtcggatagt aaggttgcgg tggcctcgga ccgaccggta
     4441 cgttcggggc acccccagga gtacttccgt ccatgaccga tgcctccccg ccgcccgacg
     4501 tccgcgcgct cctcgaggag cgggccgccg cgcgggaggc gcgcgactgg gcgacggccg
     4561 atgcgctccg cgaccggatc gccgcgctcg gatgggaggt ccaggactcc ggcggggcca
     4621 cgacggcccg acccatgctc gccaccggga cggtagaaga gctgccgtcg ctgctcgacg
     4681 ctcccgccga gctgcccctg ggcctgcagc tgctggccga ggatcacccg gacgacctgc
     4741 gccgcttcct ccgcgggctc gccgcgcatc cgccgagcgt gccctgggag ctggtgatcg
     4801 tcgccaacga gccgagctac gacctgccgg ccctcctggc cgcgttcgag ctgccgatcg
     4861 ccgtgctcga gagccatggc cgcctgggct ggggggacgc cgtcacgctg gggctgcgcc
     4921 ggtcccgcgg cgagatcacc gtcctgctcg acacctcgct ggagccgacg ggcgacgcgt
     4981 ggacgcagct cctcgccgcg ttcgacgacc cgaccgtcgg ggtcgcgggt ggctggggcg
     5041 tcaccagcgg cgatgcccgc cagttcgagg acgccccgcc cggcgaggtg gacgccgtcg
     5101 agggctactg cttggcgatt cgacgcgcgg ccctgcgcgc ggtcggcggc gtcgaccgcc
     5161 ggttccgctt ctaccgcaac gccgacctgg acctgagctt cgcggtccgc gacgccggct
     5221 ggcgcgccat gcgcaccgag cccctcccct tcgatcggca cgaacaccgc ggctggacgt
     5281 cgctgcccga cgccgagcgc gaccgtctca gcaagcgcaa cttctaccgc ttcctggagc
     5341 ggtgggggtc ccgccgtgac ctgctgctcc acccggacca gtcgtgaagg cgcccaccgt
     5401 ccacgtggtg ccgtggctgc ggccgctggg cgcgcgcgtg atccccaact ggctggccat
     5461 caccatcggc cgtcacgtct tcgcctggcg agcattgacc gatgtcgagc ttgagcacga
     5521 gctcgagcac gcgcgccagt gggcccggat cggctgggcg ttcccgctcg cgtacctcgc
     5581 ggactccatg cgcgtccgtc gacgcggcgg tcgctggtac gacgacaacc gcttcgagcg
     5641 tgaggcgcgc gcggcggcgg ccgcgcgccg gacgaacccg aaggtctagg gcgactcggc
     5701 ccaaccggcc cgatggccct cgaggcaggt gcagccctcg ggtcccacga ccgcggcgtg
     5761 ccgcgtcccg gccggcagca ccaacccgtc accggccgac agcgtcacgg gcgtctgatc
     5821 cgatcccacc aggaaggtga tggatccgtc ggcgcacacg agcagcttct cgtagccgtg
     5881 ctcgtgggcg gcgtaggtgt cgcccggtcc gttgccccac gcgtacggcg tcacgcccgc
     5941 cgcccggagc cgctcctccg cggcgaccct ccccggagcg ttcggcccgg ggagcggtcg
     6001 cagcggcggc ggcagcatca gtcgaccagg agcacggag
//
LOCUS       DASTYP010000124         7571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1575983, whole genome shotgun sequence.
ACCESSION   DASTYP010000124 DASTYP010000000
VERSION     DASTYP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575983"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFV59_05725"
     CDS             complement(<1..597)
                     /locus_tag="VFV59_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU5325365.1"
                     /translation="MKERPTLDETLDALVAVLREVRASSGRSRSELVARTGLARGAVA
                     QRVSELIDLGLVTETDVGPSTGGRPPRRIAFRGDAGHILVADIGATSIDVAVTDLEGR
                     ILGHRHQPADVAAGPDACLAAVDERFAELVRETHGIPGDLWGIGIGLPGPVEFRTGRP
                     VSPPIMPGWDGYPVRERFTDRYAAPVWVDNDVNIMALGE"
     gene            738..1898
                     /locus_tag="VFV59_05730"
     CDS             738..1898
                     /locus_tag="VFV59_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5325366.1"
                     /translation="MRRPSEIGAAVIGSGFIGTVHIEALRRIGVQVHGLLGSSPERGA
                     ASAQRIGLPRAYASLDELLADERVEVVHVTSPNHLHHPQVVQILAAGRHVVCEKPLAL
                     TSEQSGELVRLAADSGRVNAVNFNIRFYPLNQHVAGMVREGRLGDVRLVSGHYFQDWL
                     LLESDWNWRLEPDLGGSLRAVGDIGSHWLDLVSFMTGLRITEVMADLHTFIPTRHQPA
                     GPVETFSTERAANTVERKIGTEDIATILLRFENGARGTVAVSQLSPGRKNSLEYEIDG
                     SSSAVAWESELPDHLWVGHREAPNEILQRNPALMNAPGQAAAALPGGHVEGFADTFAA
                     LFRAVYADVGAGGPSERPVYPTFADGHDEMLVGDAVARSASDGRWTAVARGR"
     gene            1932..2846
                     /locus_tag="VFV59_05735"
     CDS             1932..2846
                     /locus_tag="VFV59_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU5325367.1"
                     /translation="MQLGFLTAPFPDTPLNEVADWAAGNGFEILEIACWPRASGPTRR
                     YAGTSHIDVADLSESQAKEIREEVESKGLGISGLGFYPNPMHPDRETREAAIAHMKLV
                     IEACAKMGVPYFNTFMGGDPKLHVDANWERALQIWPEIVDHATEHGVKVTIENCPMIF
                     SYDEWPAGNNIAWSPYIWRRIIETWGDTIGLNYDPSHLVWLMIDQERFIREFGPSIWH
                     VQAKDVQINRDGLYERGSLSGGMGWQIPRLPGLGDADWPKIFAALYRAGYDGAVAIEH
                     EDRDFEKTDELIKRGFLIARDVLRPYIK"
     gene            2852..3613
                     /gene="deoC"
                     /locus_tag="VFV59_05740"
     CDS             2852..3613
                     /gene="deoC"
                     /locus_tag="VFV59_05740"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5325368.1"
                     /translation="MTTSTTPRFTVDNVTERQLAKTIDHSLLKPELDDAFIEEGCRLA
                     AEYDVASVCVRPVDVARAKALLDGTDVAVGTVIGFPHGSSTTATKVFEAREALANGAT
                     ELDMVIPIGALRSGRDEDVRADIAAVVGVAHAAGAIVKVILENAYLTDDEKERGSRLA
                     EAAGADFVKTSTGFAPSGATHEDLALMRRVTSPHVQVKAAGGVRTLDDLLAVMNLGVT
                     RIGATQTKPILDDFRARRSGTGVTAGPATTDEGAY"
     gene            3615..4409
                     /locus_tag="VFV59_05745"
     CDS             3615..4409
                     /locus_tag="VFV59_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5325369.1"
                     /translation="MNAAAPPAVRWISEATALIERVAATQSDAIERASQWCADAIAAG
                     GLVHLFGTGHSRIPVEEMFPRYGSYPGFNPLVELSMTFHTQVVGANGQRQAMFIERTP
                     GLAEVILDNFRFGPHDVMVVFSASGLTAVPVEMARGARKRGLRVIAVTSVAQSMAGEP
                     SAEVGSRLLDEADLVIDLCTPHADALCDLPGLDTPVGPGSTVAAVAIVNSIKVRVAEL
                     LTERHAMPPVLTRPSVVGEERSRALFDEAYREHARRVAAIFDNARR"
     gene            4481..5602
                     /locus_tag="VFV59_05750"
     CDS             4481..5602
                     /locus_tag="VFV59_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011454587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5325370.1"
                     /translation="MVAMVAIALVLVACSGGTGSASASQPGSSDAPASQGGGDASYRI
                     GYSNGGGVGNGFREEQVCTAKAEALASDQVTELVTIHRNTDAAGQLSDIRDLIAADVD
                     AIVFNPNDPEALNPALQEAHDAGIVTVSVDAYVTDENTYNLYNNQVEYAYLGAKWLFE
                     QLDGEGTVYYMRGLAGHPADSDRDIGFKRALEEYPDIQVVPNEEGVHTGWDPATTTQL
                     INDFLASGQYADIDGIWTSGMDSQVVDAIKAAGEDYVPIVGADLGAFVAQLLDEEGYS
                     GLEGAAVTNTAAVGGAGVNLALKLLNGEDVQTAEDASQPNTVLLTPVLADNVSDEGRQ
                     LLESWQVDGLDPLWPLGLQIDGWTSYTPEQAIDCVGPGE"
     gene            5668..7206
                     /locus_tag="VFV59_05755"
     CDS             5668..7206
                     /locus_tag="VFV59_05755"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5325371.1"
                     /translation="MTTTTATDLLLEATGVSKAYGAVVALRGASLAVRRGEIHALMGA
                     NGAGKSTFVKILTGAVRPDSGTIMVAGKPRTDHSPAEARRHGVVSVYQEPSLIPDLDI
                     RSNLRLTETPVEAFRHWLSELGLERLDLSRLARRLPLASLRIIDLARAMAIEPDILLL
                     DEMTAALPANLSDRVLETIRRQGGGSRSVIFISHRMSEIGSLCNRATVLREGETVGVV
                     DVTAGSEERIVELMLGEVSQALEETEDRTGAGARISETPRLSARGISAGRHLTDVSFD
                     VYPGEVLGVVALEGQGQDELFNVLSGAARPSDGELLVDGRPVSFGHPADAIREGVVYV
                     AADRAEALLMQRSVRENIALPFAARVRRWGLIDVAEERRKVNEAIERLQIDTRAQREV
                     RRLSGGNQQKVTIARWVAGGVHTMLCFDPTRGIDIRTKQQIYRLLRDLAAAGAGVILY
                     TSELNEIRLACDRAVVIFGGRVVDELDAADADEETLLRAAHNLRRHRDATVPEQVQPE
                     AAAT"
     gene            7203..>7571
                     /locus_tag="VFV59_05760"
     CDS             7203..>7571
                     /locus_tag="VFV59_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325372.1"
                     /translation="MSARFATLRAAGANALLTDFVRRHAWTLGLLGFLVLLLIATKII
                     QPTYGVTGIQGLAISVLPLALAAAAQAVIVISGGIDLSIGSMMALTSVVAAANMDGQS
                     EGYGVLVVIGVLLLGLVLGAI"
BASE COUNT         1184 a         2676 c         2603 g         1108 t
ORIGIN      
        1 ctcgccgagc gccatgatgt tcacgtcgtt atcgacccag acgggcgcgg cgtagcggtc
       61 cgtgaaccgc tcccgaaccg ggtagccgtc ccagccgggc atgatcggcg gcgagacggg
      121 acgccccgtc cggaactcca ctggccccgg caggccgatg ccgatgcccc acaggtcgcc
      181 ggggatgccg tgcgtctcgc ggaccagctc ggcgaaccgc tcgtcgaccg ccgcgaggca
      241 ggcgtcgggg cctgccgcga cgtccgccgg ctggtgacgg tggccgagga tgcgtccctc
      301 gaggtcggtc accgccacgt cgatgctcgt ggccccgatg tcggcgacca ggatgtggcc
      361 ggcatcgccg cgaaacgcga tgcgccgcgg tgggcgcccg ccggtgctgg gcccgacgtc
      421 ggtctcggtc accagcccga ggtcgatcag ctcgctgacc cgctgggcta ccgcgcctcg
      481 cgcgaggccc gtgcgtgcca cgagctccga ccgggatcgc ccgctcgacg cgcggacttc
      541 tcgcagcacg gccaccaggg cgtcgagcgt ttcgtccagc gtcggccgtt ccttcacggg
      601 ctggatcata gcgacgcggc ggcgcacgat cgcacttttg cgcttgacgg accaaagtat
      661 gatcgggcgg tgcacccacc cgccgctgtg aacgcgacgc tcggtcgcga cctgtctaga
      721 catctagacc catcccgatg aggcgaccga gcgagatcgg ggccgccgtc atcggcagcg
      781 gcttcatcgg aacggtccac atcgaggccc tgcgccggat cggcgtgcag gtccacggcc
      841 tcctcggctc gtccccggag cgcggggcgg cgagcgcgca gcgcatcggc cttcccaggg
      901 cctacgccag cctggacgag ctgctcgccg acgagcgcgt cgaggtcgtg cacgtcacca
      961 gcccgaacca cctccaccat ccgcaggtcg tgcagatcct ggcggccggc cgccacgtcg
     1021 tgtgcgagaa gccgctcgcc ttgaccagcg agcagtcggg cgagctcgtg cgcctggcgg
     1081 cggacagcgg tcgcgtcaat gccgtcaact tcaacatccg cttctacccg ctgaaccagc
     1141 acgtggcggg catggtccgc gagggacggc tgggtgacgt tcgcctggtg agcgggcact
     1201 acttccagga ctggctgctg ctcgagagcg actggaactg gcggctggag ccggacctgg
     1261 gcgggtcgct gcgcgcggtc ggtgacatcg gctcgcactg gctggacctc gtctcgttca
     1321 tgaccgggct gcgcatcact gaggtcatgg cggaccttca caccttcatt ccgaccaggc
     1381 accagcccgc cggccctgtc gagaccttct cgacggaacg cgcggcgaac acggtcgagc
     1441 ggaagatcgg caccgaggac atcgcgacga tcctgctgcg cttcgagaac ggtgcgcgcg
     1501 ggaccgtggc ggtgagccag ctcagccctg gccgcaagaa ctcgctggag tacgagatcg
     1561 acggctcgtc gtccgcggtc gcgtgggaat cggagctgcc cgaccacctg tgggtcggcc
     1621 atcgggaggc acccaacgag atcctgcagc gcaaccccgc gctgatgaat gcgccgggcc
     1681 aggcggccgc ggcgctcccc ggcgggcacg tcgagggctt tgccgatacg ttcgcggcgc
     1741 tcttccgcgc cgtctacgcc gacgtcgggg cgggcggtcc ctctgagcgc ccggtctacc
     1801 cgaccttcgc ggacggccac gacgagatgc tcgtcggaga cgccgtggcc cgaagcgcat
     1861 cggacggccg atggaccgcg gtcgcgcggg gccgatgaca cccatgcacc acctcagagg
     1921 agcgtgatcc gatgcagctc ggcttcctga ccgcaccgtt ccccgacacg ccgctgaacg
     1981 aggtcgccga ctgggcggcc ggcaatgggt tcgagatcct ggagatcgcc tgctggccgc
     2041 gcgccagcgg ccccacgcgc cgctacgccg gcacctcgca catcgacgtc gccgacctgt
     2101 ccgagtcgca ggcgaaggag atccgcgagg aggtcgagtc gaaggggctg ggcatctccg
     2161 ggctcggctt ctaccccaac ccgatgcacc cggaccggga gacccgcgag gcggcgatcg
     2221 cccacatgaa gctcgtcatc gaggcgtgcg ccaagatggg tgtcccgtac ttcaacacct
     2281 tcatgggcgg cgaccccaag ctccacgtcg acgccaactg ggagcgcgcg ctccagatct
     2341 ggcccgagat cgtggaccac gccaccgagc acggcgtgaa ggtcacgatc gagaactgcc
     2401 ccatgatctt cagctacgac gagtggccgg ccgggaacaa catcgcctgg tccccgtaca
     2461 tctggcggcg catcatcgag acctggggcg acaccatcgg gctcaactac gacccgagcc
     2521 acctggtctg gctgatgatc gaccaggagc gcttcatccg cgagttcggc ccgtcgatct
     2581 ggcacgtgca ggcaaaagac gtccagatca accgcgacgg gctgtacgag cgcggcagcc
     2641 tgtccggcgg catggggtgg cagatccccc gcctgccggg cctgggcgac gccgactggc
     2701 ccaagatctt cgccgccctg taccgcgccg ggtacgacgg cgccgtcgcg atcgagcacg
     2761 aggatcgcga cttcgagaag accgatgagc tcatcaagcg cgggttcctg atcgcccgcg
     2821 acgtcctccg cccgtacatc aagtgaacgc catgaccacc agcacaacgc cccggttcac
     2881 ggtcgacaac gtcaccgagc gtcagctcgc caagacgatc gaccactcgc tgctcaagcc
     2941 cgagctggac gacgccttca tcgaggaggg ctgccgcctg gccgccgagt acgacgtcgc
     3001 ctcggtttgc gttcgtcccg tggacgtcgc acgcgcgaag gccctgctcg acgggaccga
     3061 tgtggccgtc ggcaccgtca tcggcttccc gcatggctcc tccacgaccg ccacgaaggt
     3121 cttcgaggcg agggaggctc tcgccaacgg ggcgaccgag ctcgatatgg tcatccccat
     3181 tggcgcgctg aggtccgggc gcgacgagga cgttcgagcc gacatcgccg ccgtcgtcgg
     3241 ggtggcccac gcggccggag ccatcgtcaa ggtgatcctc gagaacgcct acctgaccga
     3301 tgacgagaag gagcgcggca gcagactggc ggaggccgcc ggcgccgact tcgtgaagac
     3361 gtcgaccggt ttcgccccct ccggcgcgac gcacgaggac ctggcgctga tgcgacgcgt
     3421 cacctcgccg cacgtgcagg tcaaggccgc gggcggggtg cgcaccctgg acgacctgct
     3481 ggccgtcatg aacctcggcg tcacgcgcat cggcgcgacc cagacgaagc cgatcctgga
     3541 cgacttccgg gcgcggcgct ccggtaccgg ggtgacggcg ggtccggcca ccaccgacga
     3601 gggcgcgtac tgacgtgaac gccgccgcgc cgccggccgt gcgctggatc tcggaggcga
     3661 ccgcgctcat cgagcgggtc gccgccacgc agagcgacgc catcgagcgg gccagccagt
     3721 ggtgcgccga cgccatcgcc gcgggcggcc tggtccacct cttcggcacc gggcactcac
     3781 gcatcccggt cgaggagatg ttcccgcgct acggctcgta ccccgggttc aacccgctcg
     3841 tcgagctatc gatgacgttc cacacccagg tcgtcggcgc gaacggccag cggcaggcga
     3901 tgttcatcga gcggacgccg ggcctggcag aggtcatcct cgacaacttc cgcttcgggc
     3961 cgcacgacgt catggtcgtg ttctcggcct cgggcctcac ggccgtgccg gtggagatgg
     4021 cgcgcggcgc caggaagcgc ggcctgcgcg tgatcgccgt gacctccgtc gcgcagtcga
     4081 tggccggcga gccctccgcc gaggtcgggt cgcggctgct ggacgaagcc gacctggtga
     4141 tcgacctgtg cacgccccac gcggacgcgt tgtgcgatct gccggggctc gacacgccag
     4201 tcggcccggg ctcgacggtg gcggccgtgg ccatcgtcaa ttcgatcaag gtgcgcgtgg
     4261 cggagctcct gacggagcgc cacgccatgc ctcccgtcct cacccgaccc tccgtggtcg
     4321 gggaagagcg ctcgcgagcg ctgttcgacg aggcgtaccg agaacatgcc cgccgcgtgg
     4381 cggcaatctt cgacaatgcg aggaggtgag cgagaacgtg tgtcgttggg gggaaacagg
     4441 aggaggattt cagtccatga cgagggttag attccaatcc atggtggcga tggtcgccat
     4501 cgccttggtg ctcgtggcgt gttcgggcgg aaccggttcc gcttcggcgt cgcagcccgg
     4561 atcgtcggat gctccggcga gccagggcgg cggtgacgcg agctaccgca tcggctactc
     4621 gaatggtggc ggcgtcggca acggcttccg cgaggagcag gtctgcaccg cgaaggcgga
     4681 ggccctggcg tccgaccagg tcacggagct cgtgaccatc caccgcaaca ccgatgcagc
     4741 cggtcagctg tccgacattc gcgacctcat cgccgccgat gtcgacgcga tcgtcttcaa
     4801 cccgaacgat cccgaagccc tgaacccggc gctccaggaa gcgcatgacg cggggatcgt
     4861 caccgtctcg gtcgatgcct acgtcaccga cgagaacacg tacaacctgt acaacaacca
     4921 ggtcgagtac gcctatctcg gcgccaagtg gctgttcgag cagctcgatg gcgaaggcac
     4981 cgtgtactac atgcgcggcc tcgccggtca cccggccgac agcgaccgcg acatcgggtt
     5041 caagcgtgcg ctcgaggagt acccggacat ccaggtcgtt ccgaacgagg aaggcgtcca
     5101 caccggctgg gatccggcga cgaccaccca gctgatcaac gacttcctgg ccagcggcca
     5161 gtacgccgac atcgacggca tctggacgtc cggcatggac agccaggtcg tggatgcgat
     5221 caaggctgcc ggtgaggatt acgtgccgat cgtcggtgcg gacctcgggg cgttcgtcgc
     5281 tcagctcctc gacgaggagg gctactcggg cctcgagggc gctgccgtga ccaacacggc
     5341 cgccgtcggt ggcgccggcg tcaacctggc gctgaagctc ctcaacggtg aggacgttca
     5401 gacggccgag gatgcctcgc agcccaacac cgttctgctg acgccggtcc tcgcggacaa
     5461 cgtcagtgac gagggtcgtc agctgctcga gtcgtggcag gtcgacggtc tcgatccgct
     5521 gtggccgctt ggcctgcaga tcgacggctg gacgagctac acccccgagc aggcgatcga
     5581 ctgcgtcggc ccgggcgagt aggcgttccg agccacgagc gaacggggat gccggcgcca
     5641 gccggcatcc ccacgcatca ggtgaccatg accaccacca ccgcgaccga cctgctgctg
     5701 gaggcgaccg gggtctcgaa ggcctacgga gccgtcgtcg cgctgcgtgg tgcgtcgctc
     5761 gccgtccgtc gcggcgagat ccacgccctg atgggcgcga acggcgccgg caagagcacg
     5821 ttcgtcaaga tcctgaccgg tgccgtccgc ccggacagcg gcaccatcat ggtggccggc
     5881 aagccacgca cggaccactc acccgctgag gcacgccggc acggggtggt ctccgtctac
     5941 caggagccgt cgctcatccc ggacctcgac atccgctcca acctgcggct gaccgagacg
     6001 ccggtcgagg cgttccgaca ctggctctcg gagctcggcc tggagcggct ggacctgtcg
     6061 cggctggccc gccgcctgcc gctcgcctcg ctccgcatca tcgacctggc ccgggcaatg
     6121 gccatcgagc ccgacatcct gctcctagac gaaatgaccg cggcgctgcc cgccaacctg
     6181 agcgaccgcg tcctggaaac gatccgccgg cagggtggcg gctcgcgttc ggtgatcttc
     6241 atctcgcacc ggatgagcga gatcggctcg ctctgcaacc gcgcgacggt gctgcgcgag
     6301 ggtgagaccg tcggcgtggt ggacgtcacc gccggatccg aggagcgcat cgtcgagctc
     6361 atgctgggcg aggtaagcca ggccctcgag gagaccgagg atcgcacggg ggcgggggcg
     6421 cgaatcagcg aaacgcctcg cctctcggcg cgcggcatca gcgcgggccg tcacctgacc
     6481 gatgtctcgt tcgacgtcta tcccggcgag gtgctcgggg tggtcgcgct cgagggccag
     6541 ggccaggacg agctgttcaa cgtcctctcg ggagccgctc ggccgagcga cggtgagctg
     6601 ctggtcgacg gcaggcccgt ctcgttcggc cacccggccg acgccatccg cgagggcgtc
     6661 gtgtacgtgg ccgcggaccg tgccgaggcg ctcctgatgc agcgatcggt tcgggagaac
     6721 atcgcactgc ccttcgccgc gcgcgtccgt cgctgggggc tcatcgacgt cgcggaggag
     6781 cgccggaagg tcaacgaggc catcgagcgg ctccagatcg acacgcgcgc gcagcgcgag
     6841 gtgcgccgtc tgtccggcgg caaccagcag aaggtcacca tcgcgcgctg ggtcgccggt
     6901 ggcgtgcaca ccatgctgtg cttcgacccg acccgcggca tcgacatccg gaccaagcag
     6961 cagatctacc gcctgctccg cgacctcgcg gcggcgggtg cgggcgtgat cctgtacacc
     7021 tcggagctca acgagatccg tctggcgtgc gaccgcgccg tggtcatctt cggcggtcgc
     7081 gtcgtcgacg agctcgacgc ggccgatgcc gacgaggaga ccctgctccg ggcggcgcac
     7141 aacctgcgcc ggcatcgtga cgcgaccgtg cccgagcagg tccagccgga ggccgctgcg
     7201 acatgagcgc ccgcttcgcg acgctgcgcg cggccggcgc gaacgccctg ctgaccgact
     7261 tcgttcggcg ccacgcctgg acgctgggcc tgctgggctt cctggtgctg ctgctcatcg
     7321 ccacgaagat catccagccg acgtacggcg tcaccggcat ccagggactc gccatctcgg
     7381 tcctgccact ggccctggcg gccgcggcgc aggcggtgat cgtgatctca ggcggcatcg
     7441 acctctccat cggctcgatg atggccctca ccagcgtggt cgccgccgcg aacatggacg
     7501 ggcagagcga gggctacgga gtcctggtcg tcatcggcgt gctgctgctc ggcctggtcc
     7561 tcggcgcgat c
//
LOCUS       DASTYP010000125         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1283888, whole genome shotgun sequence.
ACCESSION   DASTYP010000125 DASTYP010000000
VERSION     DASTYP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283888"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1233)
                     /locus_tag="VFV59_05765"
     CDS             complement(76..1233)
                     /locus_tag="VFV59_05765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5325373.1"
                     /translation="MTEPPEHPRAPDGLLIALTVATNGGVLVLGLVAAWWVGAHLPNP
                     LAAWDRWDAPHYTDIAVFGYMANDPGNLVGPNGYRQVYPGDLDLYIVFFPLYPWLVGA
                     VNAVLQAPVASAFVVSGVASLFVAPLLYRLVAADLGGRIGLWAAAFLLTFPTAYFLHI
                     GYTESLFLALAFGSMWLARTNRWWAAGVLGGLAALTRANGLVLLPTLAVEAYLQWRVD
                     RRVRLQWLAIGGVAIGFAVYLGVNAAVYGDPFAFSLIQREHWFKDLSPPWEGIAGMIG
                     WTANAEADTAFMLGWMELLFTGIGLAATVLTALWLRPSWAVWMAGNWLLITGTGFVMS
                     VPRYVLVLFGIFVWMAMAAQRWPWVGWLLAALSVASMGYFAWRFAIGWWAF"
     gene            1305..1381
                     /locus_tag="VFV59_05770"
     tRNA            1305..1381
                     /locus_tag="VFV59_05770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1339..1341,aa:Pro,seq:cgg)
     gene            1428..>3173
                     /locus_tag="VFV59_05775"
     CDS             1428..>3173
                     /locus_tag="VFV59_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5325374.1"
                     /translation="MPAPPTTAAEHRVEHGGELITTIALGLSVAFVAGFLARRLRLPP
                     IVGYLLAGVALGPFTPGLFADQAVATELAEIGVILLMFGVGIHFSFRDLLAVRGIAIP
                     GAIVQSTVATVLGIGLAMLIGWGVAAGVVLGLSLSVASTVVLLRALIQRNALDTVHGR
                     AAVGWLIVEDIFTVLVLVLLPGFALVVQGSGGSGVDVVLALGEALLKAAVLGVLMVVV
                     GARVVPWLLLQVAREGSQEMFTLAVLAIALGIAFASYAIFGVSLALGAFLAGAVVGES
                     DVSHKAAADALPLRDAFSVLFFVSVGMLLDPAYLVANPLVVLAVVALIVIGKSVAAVG
                     IVAVLGYPVRTGLTVGAGLAQVGEFSFILATAGAALDLLPREAYQLVVAGALISITLN
                     PLMFALVDPLDAWLKRHPALRGRLDHGGGRLALPADSQEPMSGHAVICGWGRVGRTVA
                     RALEARSFRFVVVEEDRRTVEALRKRGIQALYGDIADAQLLHHAGIERARVLVFAAYD
                     PPAAEFAVEHARSVNPRIEIVARVETPEEARRLLDRGATQTVEGERELAVQMARYTLR
                     RFGVTSREVDVITQGL"
BASE COUNT          441 a         1133 c         1135 g          464 t
ORIGIN      
        1 tggcagacct gagatcacct caacggtcgc cgcggttcct tcgggacatt tcgttgtccg
       61 ccgaacggcg caacctcaga acgcccacca gccgatggcg aagcgccacg cgaagtagcc
      121 catggaggcc acggacagcg cggccagcag ccagccgacc cacggccagc gctgcgcggc
      181 cattgccatc cacacgaaga tcccgaacag caccagcacg tagcgcggga cgctcatcac
      241 gaacccggtg cccgtgatca ggagccagtt gcccgccatc cacaccgccc agctcggccg
      301 cagccagagc gcggtgagca ccgtcgcggc cagcccgatc cccgtgaaca gcagctccat
      361 ccagccgagc atgaaggccg tatcggcctc ggcgttggcc gtccagccga tcatcccggc
      421 gatgccctcc catggcgggg agaggtcctt gaaccagtgc tcgcgctgga tcaggctgaa
      481 ggcgaacggg tcgccgtaga ccgcggcgtt gacgcccagg tagaccgcga agccgatggc
      541 gacaccgccg atcgccagcc actggagccg cacgcgccgg tcgacgcgcc actggaggta
      601 ggcctcgacc gccagtgtcg gcagcagcac caggccgttc gcccgcgtca gcgccgcgag
      661 cccgccgagc accccggccg cccaccatcg gttggtccgc gccagccaca tcgagccgaa
      721 ggcgagcgcc aggaagaggc tctcggtgta gccgatgtgc aggaagtacg cggtcgggaa
      781 ggtcagcagg aaggccgcgg cccacaggcc gatgcggccg ccgaggtcgg ccgccaccag
      841 ccggtacagc aggggcgcga cgaacagcga ggccacgccc gacacgacga acgccgatgc
      901 caccggcgcc tggagcaccg cgttcacggc cccgaccagc cacgggtaca gcgggaagaa
      961 gacgatgtac aggtcgaggt cgccggggta gacctgccgg tagccgttgg gcccgaccag
     1021 gttgcccggg tcgttggcca tgtagccgaa gaccgcgatg tcggtgtagt gcggcgcgtc
     1081 ccatcggtcc caggcggcca acgggttcgg caggtgcgcg ccgacccacc aggcggccac
     1141 caggcccaaa accagcacgc cgccgttcgt ggccacggtc agcgcgatga gcagcccatc
     1201 aggcgcgcgt ggatgctcgg gcggctcggt catcggcgcg actatagggg agggcccggc
     1261 ggtggaccgt cggcccttcg gctgctacca tcggagcctc cggtcggggc gtggcgcagc
     1321 ttggtagcgc gcgtcgttcg ggacgacgag gtcggaggtt caaatcctct cgccccgacc
     1381 attctcatca cgccggccgt tgagcgcacg acgcacgacc gacgccgatg ccggctccac
     1441 ccaccacggc agcggagcat cgcgtggagc acggcggcga gctgatcacc accatcgccc
     1501 tcgggctgag cgtggccttc gtcgccggct tcctggcgcg gcgcctgcga cttccgccca
     1561 tcgtcggcta cctgctggcc ggtgtcgccc tcggcccgtt cacccccggc ctcttcgccg
     1621 accaggcggt cgcgaccgag ctggccgaga tcggcgtgat cctgctgatg ttcggcgtcg
     1681 gcatccactt cagcttccga gatctgctgg cggtgcgcgg catcgcaatc ccgggcgcca
     1741 tcgtccagag caccgtcgcg acggtgctcg gcatcggcct ggcgatgctc atcggctggg
     1801 gcgtcgccgc cggcgtcgtg ctgggcctct cgctgtcggt cgcgagcacg gtcgtcctgc
     1861 tgcgggccct catccagcgc aacgccctcg acaccgtgca cgggcgcgcg gccgtgggct
     1921 ggctcatcgt ggaggacatc ttcaccgtgc tggtgctggt cctcctgccc ggcttcgcgc
     1981 tggtggttca ggggtccggc gggagcggcg tggacgtggt gctggcgctc ggcgaggcgc
     2041 tgctcaaggc cgcggtactg ggcgtgctga tggtggtcgt cggagcgcgc gtggtgccct
     2101 ggctgctgct gcaggtcgcg cgcgaggggt cgcaggagat gttcaccctg gccgtgctgg
     2161 cgatcgcgct cgggattgcc ttcgcgtcct acgcgatctt cggcgtgtcg ctggcgctcg
     2221 gcgccttcct ggcgggggcc gtggtgggcg agagcgacgt ctcgcacaag gctgcggcgg
     2281 acgccctgcc gctccgggac gccttcagcg tcctcttctt cgtctcggtc gggatgctgc
     2341 tggaccccgc ctacctcgtc gcgaatccgc tggtggtgct ggccgtcgtg gcgctgatcg
     2401 tcatcggcaa gtccgtggcc gccgtcggca tcgtcgccgt gctggggtac ccggtgcgca
     2461 ccgggctcac cgtgggggcg ggcctggcgc aggtcgggga gttcagcttc atcctggcga
     2521 cggcgggcgc ggcgctcgac ctgctgccgc gagaggcgta ccagctcgtc gtggccggcg
     2581 cgctgatctc gatcaccctg aacccattga tgttcgcgct ggtggacccg ctcgacgcgt
     2641 ggctcaagcg ccatccggcg ctccgcggcc ggctcgatca cggcggtggg cgcctggcac
     2701 tgccggccga cagccaggag ccgatgagcg ggcacgccgt gatctgcggc tggggccgtg
     2761 tcggacgcac cgtggcgcgg gccctcgagg cccggtcgtt ccgcttcgtg gtcgtggagg
     2821 aggatcgccg gaccgtggag gcgctgcgca agcgcggcat ccaggcgttg tacggcgaca
     2881 tcgccgatgc gcagctgctg caccacgccg gcatcgagcg ggcccgcgtc ctggtcttcg
     2941 ccgcctacga ccctccggcc gccgagttcg ccgtcgagca cgcccgcagc gtcaatccgc
     3001 ggatcgagat cgtggcgcgc gtcgagacgc cggaggaggc ccgtcgcctg ctggaccgcg
     3061 gcgcgaccca gacggtcgag ggcgagcgtg agctggcggt gcagatggcc cgatacaccc
     3121 tgcgccgctt cggcgtcacc agccgggagg tcgacgtcat cacccagggc ctg
//
LOCUS       DASTYP010000126        15088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_82417, whole genome shotgun sequence.
ACCESSION   DASTYP010000126 DASTYP010000000
VERSION     DASTYP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15088
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82417"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VFV59_05780"
     CDS             <1..1294
                     /locus_tag="VFV59_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5325375.1"
                     /translation="VADLAGGVDSIEVRRLLDVPDARYEPIGAVDGTLYLLTDRDAPN
                     GRVVSADAAGDAAADAQPVLRELIGEGADAIEQVVLAGGRLAVLTLHDAHHRLAIHEL
                     DGTFVTQVALPAIGTVAELAGRREDDELFVTFLTFAAPASVLAVRMADGSVRTVSRPS
                     LGWDPDAYVTEQVFVTSDDGTRVPLFLTHRGDVTPSGDVPTLLYGYGGFQVPVGPTFK
                     PEWLAWMERGGLLAVASLRGGAEYGKAWHDAGRLANKQNVFDDFAACARWLAASGWTR
                     RQRIGILGRSNGGLLVGASITQHPELFGAAVAEVGVMDMLRFHRFTIGWGWTSDYGSA
                     DDPEQFEWLRAYSPLHNVRPGVAYPPTLVTTGDHDDRVVPGHSFKFAAALQAAQDADA
                     PVVIRIDTDAGHGVGKPVSKLIDERADVLAFLELALEG"
     gene            complement(1316..2371)
                     /locus_tag="VFV59_05785"
     CDS             complement(1316..2371)
                     /locus_tag="VFV59_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5325376.1"
                     /translation="MTTPDAAAAFEALLARRGGDAVLLLDASLRVVRAGDEVAALAER
                     PVHALAGMSLIAALGSASVDSLARRAASTGDAQSGEADLGHLGARTFAVDALPIDGGG
                     VVLSLHDITALRRMERVRRDFVANTGHELRTPLTSIKLLAETLASGAGEDATLTAEFA
                     TQIERETDHLVQLVDELLELTRIESGETDLVLERADPSDLVAACAARIGPVAQRREVS
                     VRVGPDAAGEVAVRVDRQRLGQALLNLAHNAVKFSHPGGEIRLSWERTDGHVRFAVAD
                     DGVGIPEQHQPRVFERFYKVDRSRTPERDAGMPGGSAGLGLSIVRHIAEAHGGSVGMT
                     SEEGVGSTFWIEVPVAV"
     gene            complement(2368..3069)
                     /locus_tag="VFV59_05790"
     CDS             complement(2368..3069)
                     /locus_tag="VFV59_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5325377.1"
                     /translation="MRRRLLVVEDDATLGQTLRFSLAREGYDVSVATTGPAALDAVRA
                     RRCDLVLLDVMLPGMSGVEVLRVMRQEGIETPVIIVSAKTEELDRVVGLKVGADDYVT
                     KPFSRAELLARIEAVLRRGRRAEAEEATASEVLEFDGLRIDPERREVTLAGEALHLTT
                     KEFDLLAHLAASPGRIFTRDQLLSRIWGFDYEGDTRTVDVHLSWLRTKLRGHGGHNWF
                     RTVRGVGYAFAPPEP"
     gene            complement(3082..3780)
                     /gene="phoU"
                     /locus_tag="VFV59_05795"
     CDS             complement(3082..3780)
                     /gene="phoU"
                     /locus_tag="VFV59_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5325378.1"
                     /translation="MEMERTAQTESEHLGPRATFDRALDRVKDDVLRLGAMVEDALAR
                     AGRALVERDSELADRVRWDDARVNDLQRDINTEITTLIATQAPVARDVRELLALYHAS
                     AELERMGDYAVNIAKLAQQLAAEPETPILKQIPRMEQVCRSQLRAAMRALVDVSEADA
                     RAVCQRDDEIDHLYNSVYEDAVGIMQTEPERARQAIHMLFAAHHLERLGDRVTNIGED
                     VVYLATGQVEDLNP"
     gene            complement(3791..4576)
                     /gene="pstB"
                     /locus_tag="VFV59_05800"
     CDS             complement(3791..4576)
                     /gene="pstB"
                     /locus_tag="VFV59_05800"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019870431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU5325379.1"
                     /translation="MPKPGEEADAVFELRDMSVHYGAFRAVRDLSLTVRRNRITALIG
                     PSGCGKSTVLRSLNRMNDLVPGARVEGTVLYDGQDIYAPDVDAVEVRRRIGMVFQKPN
                     PFPKSIYDNVAFGPRLNGFRGDLDGLVEEALRRAALWDDVKDKLRQNALSLSGGQQQR
                     LCIARAIAVEPDVILMDEPASALDPIATLRIEELMRELKENYTIVIVTHNMQQAARAS
                     DETAFLTMGDDRAGYLVEMAPTEQLFTRPASQLTEDYITGRFG"
     gene            complement(4663..5541)
                     /gene="pstA"
                     /locus_tag="VFV59_05805"
     CDS             complement(4663..5541)
                     /gene="pstA"
                     /locus_tag="VFV59_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5325380.1"
                     /translation="MSGRSLALRSGHSRRRDRFDLVMRGVLLLAVLLAVVPLASILVV
                     VVTNGLGAMSWEFLTTTQPLSFRQEGGGFLHGLVGTAMMLGIAILIAVPLGIAAAVFL
                     TEYPEHRLTRPVRFFTDVMTGVPSIFVGLFVYAALVREAGLGFGTLPGGVALSILMLP
                     IVVRSAEEILRLVPADLRSASTAMGARRWQTTLFVVIPAAASGLVTGVMLAVARGAGE
                     TAPLILTAFGTPYLVSALTGEPQTALPLLIFEGARLPFPAAQARAWAGALELMALVLV
                     LTLLARWIGGRNRYGR"
     gene            complement(5538..6518)
                     /gene="pstC"
                     /locus_tag="VFV59_05810"
     CDS             complement(5538..6518)
                     /gene="pstC"
                     /locus_tag="VFV59_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU5325381.1"
                     /translation="MASRPATAAVRGIARGRLGDRAFRWLVTASGALVLAILAWMIIS
                     TTLESWPVFAKEGLGFFTSTDWRPGASRTDVTGDYGALAFLFGTVVTSLIATLIAVPL
                     AVGIALYLVEVAPRWLRGPLTATVDLLAMIPSVVVGLWGVLFFVPVVVFPVMDALAST
                     IGTVVPLFAGPPIVRSIFAAGVVLALMVLPIVTAIVREVFATTPVDERNAAFGLGATR
                     WEVMRHVVLPRARAGIIGATMLGLGRALGETIAVLLVIGGSAQITINLFQPGQTIAGQ
                     IASGFGEASPEGIRGLIALGVALFAITILINVAARAIVWRIGRPSGDSAL"
     gene            complement(6520..7611)
                     /gene="pstS"
                     /locus_tag="VFV59_05815"
     CDS             complement(6520..7611)
                     /gene="pstS"
                     /locus_tag="VFV59_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU5325382.1"
                     /translation="MTPTTRRPLIAAGLGLTLTIAACGGGTGTSDDSPASSGGGTGLS
                     ADLVGAGASFPEPIYLEWIGQYQDVEPGVTINYQGIGSSGGREQFIAGQVDFAGSDAF
                     MKDDEIAAALDASGCSDVLHIPTVFGGVAIAFNVPGVDQLTLDGETIAKIALGDITNV
                     NDPAIASLNPDVDLPDQALTWVHRSDGSGTTSIFTKYLEDVSPDWADQVGSGSEVEWP
                     TGIGGDQNDGVAAAISQQPGGIGYVSYEFANEAGLAVAAVVNADGNAVVPSTDSVSEA
                     ANTVEVPDDFRFNILGVGGDGYPIAGATWILAYSCGMEPAQADALKGFLTWALTEGDA
                     TATELNYAPLPDELQARVLEQVDRINEEG"
     gene            complement(7708..8709)
                     /locus_tag="VFV59_05820"
     CDS             complement(7708..8709)
                     /locus_tag="VFV59_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU5325383.1"
                     /translation="MLEGITLTLLIVLALSVAFDYINGFHDTANAIATSVSTRALAPG
                     WAIVMSAVANFVGALTGTAVAHTVGAGLIDTQVESQAVIAAALVGAIAWNLLTWRLGI
                     PSSSSHALIGGLLGAALTASGLGAWQMDGIVNKVLVPLVGSPIVGFIGGLLLMALIFN
                     IFRRAHPARLNAVFRRLQVLSAAYMAFSHGSNDAQKTMGIMTLALVTAGVIPSFEVPL
                     WVILVAASAISLGTAAGGWRIIKTMGTKVVKLDPVHGFAAETTAATVIFGASHFGMPV
                     STTHVISSAIMGVGSSDRLKTVRWGVARSIVTAWILTLPASGLAAALAYLVIKPLFG"
     gene            complement(8702..9319)
                     /locus_tag="VFV59_05825"
     CDS             complement(8702..9319)
                     /locus_tag="VFV59_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU5325384.1"
                     /translation="MRLPFIPREEKFFDLFVEDAQNVLAGARMLEELFRSYDQRERLA
                     SQLRDTERRGDGISHDIGQRLAKTFVTPFDREDIHQLISRLDDVIDFIEEVADTCILY
                     KIDAPTQTATEQARIVVLQCEEMVRALEKLRGFKGVEPHWIEIHRLENEGDRIARQAI
                     ADLFMNGADPVHVIKWKDVYGLLEDTIDACEDVANVIESIVVKHA"
     gene            9409..10323
                     /locus_tag="VFV59_05830"
     CDS             9409..10323
                     /locus_tag="VFV59_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU5325385.1"
                     /translation="MRRVLLWMARNRMLRDLVPRLWFAKRAVGRFMPGESMDEALDAA
                     ERYRHDGIGAIFTHLGENLADLSEADAVATHYHELLDRIVARGIDGEISVKLTQLGLD
                     LDREACARHLGALAARAREIGAGTVWIDMEGSAYTETTVALYEQLKPEHPNLGICLQA
                     YLKRTAADITRLGPLRPSIRMVKGAYDEPARIAYRSRSEVDASFVSQSVVILRDGAER
                     LGLGTHDVRLIEQIATSADVAGISRDAYEVQMLYGIRTDQLRRLSKAGYRARVLIAYG
                     EFWYPWYVRRLAERPANVLFVLRQLLPW"
     gene            complement(10331..10645)
                     /locus_tag="VFV59_05835"
     CDS             complement(10331..10645)
                     /locus_tag="VFV59_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009069003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU5325386.1"
                     /translation="MNDAWLTVLVVGVGTVVIKAAGPVLLGGRPLPRAVDAVVSLLAP
                     ALLAALVATATLGQGQALVADARLIGIGAAVVALALRAPVLVTVVVAAATAALARLAG
                     IG"
     gene            complement(10642..11313)
                     /locus_tag="VFV59_05840"
     CDS             complement(10642..11313)
                     /locus_tag="VFV59_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU5325387.1"
                     /translation="MSDRSPRPGVRHGARDALPLAVAVGLFGVSFGVLATSGHGLGGV
                     AAVVMSATTFAGSAQFAAASVLTTGGQPIAAIAAALLLNARYLPIGISIARALRGSAL
                     ARFVHAQLVVDESWAIASLGPGHFDAGRLVGAGMTIWLAWVAGTIIGVVGGESLGDPA
                     ALGLDAAFPALFLALLAGQVRSRRAITAALLGGGIALVLVPFTPPGVPIVAAAAGALV
                     GLVRR"
     gene            complement(11310..12371)
                     /locus_tag="VFV59_05845"
     CDS             complement(11310..12371)
                     /locus_tag="VFV59_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5325388.1"
                     /translation="MRLPGSIGVRIAVAALAVALVTLAIVTVGVIVFGGNAFMDLMTA
                     HGETAEDARSMFESTVLVVLIVALVVAIGLATALAIAAGRWVAQPLRDLGGAARRIAE
                     GDYGAQLDRRGFDELASLTDSFNQMSTGLLEQERMRREFIANAAHELRTPLTNLKGYL
                     EALRDGVMPADRATFESLWDETERLVRLSESLDRLEEGPRPSARTEIDVSRAVETAVA
                     LARPSATVRGLRWQLDLPTSLHARADPDHLAQVLANLLQNAVRYTPEGGTVTVRAEEQ
                     RDDVLVSVLNTGTTIPADDLGRVFERFYRLDRSRDRRSGGAGIGLAIVKQLVEMAGGR
                     VGARSERGTTTFWFSLPKA"
     gene            complement(12368..13096)
                     /locus_tag="VFV59_05850"
     CDS             complement(12368..13096)
                     /locus_tag="VFV59_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5325389.1"
                     /translation="MLAVSTGGDERPILVVDDDPKIVHLVRTYLEREGHRVTAAHDGR
                     SALASMALDPPALVVLDLMLPEVDGISVLRAIRRRDATPVVVLSARGTARDRVEGLEE
                     GADDYVPKPFSPAELVLRIRRLLRTPDPRPQTHEPIRHGALVVDRDRRRVTVDGAPVE
                     LTRIEMELLLALLDADGRVLTRDQLLDAVHGRGEAFVLDRTVDAHIKRLRAKLSDDPD
                     APRFVATVRGVGYRAVEADEGRTP"
     gene            complement(13137..13550)
                     /locus_tag="VFV59_05855"
     CDS             complement(13137..13550)
                     /locus_tag="VFV59_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325390.1"
                     /translation="MRRLVAAMATAVFVTALAVSTVAAAAGPPGLAFYVDGERYRTVA
                     TPTDLSDTGAPEQSYDRIYALGNGLINVAEAKPGDRDYNGGRWIVLGIEWHVAPVQLT
                     SAEQVEAYAVMGKLTIGTDPLKMFVCTVNRVPAGQ"
     gene            13796..14332
                     /locus_tag="VFV59_05860"
     CDS             13796..14332
                     /locus_tag="VFV59_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5325391.1"
                     /translation="MTTTEMAVTPPTSRWIEAERATLREVLMGAQDNLTNVLAVVLGV
                     SIGAGRADLVGLAGLSAAVAEAISMGGVLYTATRAEADLEPDRASRLSPVAAGVVCAV
                     AALLGGLVPLAPFLLLPIGLAVVASLAMSLLALFVLGAWSATVTRRTWWRDGARLVLI
                     AGAAALAAALIGHGLRVD"
     gene            complement(14343..>15088)
                     /locus_tag="VFV59_05865"
     CDS             complement(14343..>15088)
                     /locus_tag="VFV59_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5325392.1"
                     /translation="MYVERVGETGPRLLLVHGGIVPGWQTWEAQRVLAEEYRLVVPHR
                     PGYPPNAPGEGIQFDAQARAVAELIEPGSHVIGHSYGGVVSMLATALVPDRVRSLTVI
                     EPPAFGLVRGIPAVEELIARLEPLFADHELPAREFFLAFAATVGATPRLPETLPPETE
                     ASILASRLEPLPWTASIPLDEVAAAGMPTLVFSGGHHAAFDAVCDHIAERLGSEREVI
                     TGRAHSVQRTGEPFNARLRAFLDSVLGAA"
BASE COUNT         2180 a         5369 c         5445 g         2094 t
ORIGIN      
        1 ggtggccgac ctcgccggcg gggtggactc gatcgaggtc cggcgcctgc tcgacgtccc
       61 cgatgcgcgc tacgagccga tcggcgccgt cgacggcacc ctctacctgc tgacggaccg
      121 cgacgcgccg aacgggcgcg ttgtctcggc cgatgcggca ggcgatgcgg cagccgatgc
      181 gcagccggtg ctgcgcgagc tcatcggcga gggcgccgac gcgatcgagc aggtcgtgct
      241 ggccggggga cgcctggcgg tcctcacgct ccacgacgcg caccaccgcc tcgcgatcca
      301 cgagctcgac ggcaccttcg tgacccaggt cgccctgccc gccatcggga ccgttgcgga
      361 gctggccggg cgccgcgagg acgacgagct gttcgtgacc ttcctcacgt tcgcggcgcc
      421 cgcatcggtc ctggccgttc ggatggccga cggctcggtg cgaaccgtgt cacggccgtc
      481 attggggtgg gatcccgacg cgtacgtgac cgagcaggtc ttcgtgacct ccgacgacgg
      541 cacgcgcgtg ccgctgttcc tcacccatcg cggcgacgtg acgccgagcg gcgacgtccc
      601 gacgctgctg tacgggtacg gcgggttcca ggtgcccgtc gggccgacct tcaagcccga
      661 gtggctggcc tggatggagc gcggcggtct gctggcggtc gcctcgctgc gtgggggcgc
      721 cgagtacggc aaggcatggc acgacgccgg tcggctggcc aacaagcaga acgtgttcga
      781 cgacttcgcc gcctgtgccc gctggctggc ggcatccggc tggacgcgcc gccagcggat
      841 cgggatcctg ggacgctcga acggtggact gctggtgggg gcgtcgatca cgcagcaccc
      901 ggagctgttc ggcgcggccg tcgccgaggt tggggtcatg gacatgctgc gcttccaccg
      961 cttcaccatc ggctggggct ggaccagcga ctacggctcc gccgacgacc cggagcagtt
     1021 cgaatggctg cgcgcctact cgccgctgca caacgtccga ccgggcgtcg cgtacccacc
     1081 caccctggtg accaccggcg accacgacga ccgggtcgtg cccggccact cgttcaagtt
     1141 cgcggccgcc ctgcaggcgg cgcaggacgc ggacgcgccg gtggtcatcc ggatcgacac
     1201 cgatgctggc cacggcgtgg ggaagccggt cagcaagctg atcgacgagc gggccgacgt
     1261 gctcgccttc ctggagctgg cgctggaggg ctgacggcgc tgctcgcgac gtcggtcaga
     1321 ccgcgaccgg cacctcgatc cagaacgtgg agcccacgcc ctcctccgac gtcatgccca
     1381 ccgagccgcc atgggcctcg gcgatgtgcc ggacgatcga gagccccagg ccggcgctgc
     1441 cgccgggcat cccggcgtcg cgctccggcg tgcgcgaccg atcgaccttg tagaagcgct
     1501 cgaacacgcg cggctggtgc tgctcgggga tgccgacgcc gtcgtcggcg acggcgaagc
     1561 gcacgtgccc atcggtccgc tcccacgaca gccggatctc accgccgggg tggctgaact
     1621 tcacggcgtt gtgggcgagg ttgaggagcg cctgccccag ccgctggcgg tcgaccctga
     1681 ccgcgacctc gccggcggcg tccggcccca cgcggacgga cacctcgcgt cgctgggcga
     1741 cgggaccgat gcgcgccgcg caggcagcca ccaggtccga cggatcggca cgctcgagca
     1801 ccaggtccgt ctcaccggac tcgatccggg tcagctccag cagctcgtcc acgagctgaa
     1861 cgaggtggtc cgtctcgcgt tcgatctggg tcgcgaactc ggcggtgagc gtcgcatcct
     1921 cgcccgcgcc cgaggcgagc gtctcggcca ggagcttgat cgaggtgagc ggggtgcgca
     1981 gctcgtggcc ggtgttggcc acgaagtcgc gccgcacacg ctccatccgc cgcagcgcgg
     2041 tgatgtcatg cagcgagagg accacgccgc ccccatcgat cggcagcgcg tcgaccgcga
     2101 aggtccgcgc cccgaggtgg cccaggtccg cctcgccgct ctgcgcatcg ccggtcgagg
     2161 cggcacggcg ggccagcgag tcgaccgatg ccgaccccag cgccgcgatc aggctcatgc
     2221 ccgccagcgc gtggaccggg cgctcggcca gggcggccac ctcgtcgccg gcgcggacga
     2281 cccgcaggct ggcgtcgagg agcaggacgg catcgccgcc acgccgcgcc aggagcgcct
     2341 cgaaggcggc ggccgcgtcc ggcgtcgtca cggctcgggc ggcgcgaacg cgtagccgac
     2401 cccccgcacc gtgcggaacc agttgtggcc gccgtgcccg cgcagcttgg tgcgcagcca
     2461 gctgaggtgg acgtcgacgg tgcgcgtatc gccctcgtag tcgaagcccc agatgcgcga
     2521 cagcagctgg tcccgcgtga agatgcggcc gggtgacgcc gccaggtgcg ccaggaggtc
     2581 gaattccttg gtggtcaggt gcagcgcctc gcccgccagc gtcacctcgc gtcgctcggg
     2641 atcgatccgc agcccgtcga actcgagcac ctccgacgcg gtcgcctcct ccgcctcggc
     2701 ccgccgtccg cgccgcagca ccgcctcgat ccgtgccagc agctccgcgc ggctgaacgg
     2761 cttggtcacg tagtcgtccg cgccgacctt gaggccgacc acgcggtcga gctcctcggt
     2821 cttggccgag acgatgatca ccggcgtttc gatgccctcc tgccgcatga cgcgcagcac
     2881 ctcgacgccg ctcatcccgg gaagcatcac gtccagcagg acgaggtcgc agcggcgggc
     2941 acgaacggca tcgagcgccg cgggaccggt cgtcgcgacg ctcacgtcgt acccctcacg
     3001 agcgaggctg aagcgcaggg tctggccgag cgtggcgtcg tcctcgacga cgagcaggcg
     3061 gcggcgcatc ggctcacccc ctcaggggtt gagatcctcg acctggccgg tggccaggta
     3121 gaccacgtcc tccccgatgt tcgtcacgcg gtcgccgagc cgctccaggt ggtgcgcggc
     3181 gaagagcatg tggatggcct gtcgcgcgcg ctcgggctcg gtctgcatga tcccgacggc
     3241 gtcctcgtag acgctgttgt agaggtggtc gatctcgtcg tcgcgctggc agacggcgcg
     3301 cgcgtccgcc tcgctgacgt cgaccagcgc gcgcatcgcg gcgcgcagct gcgagcggca
     3361 cacctgctcc atgcgcggga tctgcttgag gatcggcgtc tcgggctcgg cggccagctg
     3421 ctgcgcgagc ttcgcgatgt tcaccgcgta atcgcccatg cgctcgagct cagccgaggc
     3481 gtggtacagc gccagcagct cgcggacgtc gcgcgcaacc ggtgcctgcg tggcgatgag
     3541 cgtcgtgatc tccgtgttga tgtcgcgctg caggtcgttg acgcgggcgt cgtcccagcg
     3601 aacgcggtcg gccagctcgc tgtcgcgctc caccagggcg cgtccggcgc gcgccagcgc
     3661 gtcctcgacc atcgcgccga ggcgcagcac gtcgtccttc acccgatcca gcgcgcgatc
     3721 gaacgtggcg cgcggtccga ggtgctcgga ctccgtctgc gcggttcgct ccatctccat
     3781 cgggctcctc ctagccgaac cgaccggtga tgtagtcctc ggtcagctgg ctcgccggcc
     3841 gcgtgaacag ctgctccgtc ggcgccatct cgaccaggta gccggcccgg tcgtcgccca
     3901 tggtcaggaa cgccgtctcg tcactggcgc gcgccgcctg ctgcatgttg tgcgtgacga
     3961 tcacgatcgt gtagttctcc ttgagctcac gcatcagctc ctcgatccgc agcgtcgcga
     4021 tggggtcgag cgcgctggcc ggctcatcca tcaggatcac gtccggctcg accgcgatgg
     4081 cgcgcgcgat gcacagccgc tgctgctggc caccggacag cgacagggcg ttctgccgga
     4141 gcttgtcctt gacgtcgtcc cacagcgcgg cgcgccggag cgcctcctcg accagcccgt
     4201 ccaggtcgcc gcggaagccg ttgaggcgcg gcccgaaggc gacgttgtcg tagatgctct
     4261 tcgggaacgg gttcggcttc tggaacacca tgccgatgcg ccggcgcacc tcgaccgcgt
     4321 cgacgtccgg cgcgtagatg tcctggccgt cgtagaggac cgtcccctcg acccgcgcgc
     4381 ccggcacgag gtcgttcatg cggttcaggc ttcgcagcac ggtcgacttg ccacagccgc
     4441 tggggccgat gagggcggtg atgcggttgc gtcggacggt cagtgagagg tcgcgcaccg
     4501 cgcggaaggc gccgtagtgc acggacatgt cgcgcagctc gaagaccgca tcggcctcct
     4561 cgccgggctt cggcatgggc gcgccctggc ggacatcggt gtcgtcggtg ggtctgattt
     4621 ggatggtcaa ctcgatcccc gtgcgctcgc ctgcctgaag tgtcatcgac cgtatcggtt
     4681 ccgtccaccg atccaacgcg cgagcagcgt caggacgagg acgagagcca tgagctcgag
     4741 ggcgccggcc cacgctcggg cctgggccgc cgggaaggga agccgcgccc cctcgaagat
     4801 gaggagcggc agcgccgtct ggggctcgcc ggtcagggcg ctgaccaggt agggcgtgcc
     4861 gaaggcggtg aggatcagcg gcgcggtctc gcccgcgccg cgcgcgacgg ccagcatcac
     4921 gccggtgacc agccccgagg cggctgcggg gatgacgacg aagagggtcg tctgccagcg
     4981 acgcgccccc atcgccgtgc tggcactgcg caggtccgcc ggcaccagcc gcaggatctc
     5041 ctccgcgctg cgcaccacga tcggcagcat caggatcgac agcgcgacgc cgcccggcag
     5101 cgtgccgaag ccgaggccgg cctcccgcac cagcgcggcg tagacgaaga ggccgacgaa
     5161 gatcgacggg acgccggtca tgacgtcggt gaagaagcgt accggccgcg tcagccggtg
     5221 ctcggggtac tcggtcagga agacggccgc cgcaatcccc agcggcacgg cgatcaggat
     5281 ggcgatcccg agcatcatcg ccgtcccgac cagcccgtgg aggaagccgc caccctcctg
     5341 ccggaaggag agcggctgcg tggtggtcag gaactcccag ctcatggccc ccaggccgtt
     5401 cgtgacgacc acgaccaaga tcgaggccag cggcacgacc gccagcagca ccgcgagcag
     5461 cagcacgcca cgcatgacca ggtcgaaccg atcccgccgc cgggagtgcc cgctccgcag
     5521 cgccagcgac cggccgctca cagcgcgctg tccccgctcg gtcggccgat gcgccacacg
     5581 atggcgcgtg ccgccacgtt gatcaggatg gtgatcgcga acagcgcgac gcccagggcg
     5641 atgagacccc ggatgccctc gggactggcc tcaccgaagc cgctggcgat ctgcccggcg
     5701 atggtctgcc ccggctggaa caggttgatg gtgatctgtg ccgagccgcc gatgaccagc
     5761 agaacggcga tggtctcacc gagcgcccga cccagcccga gcatggtcgc gccgatgatc
     5821 ccggcccggg cacgtggcag caccacgtgg cgcatcacct cccagcgagt cgcgccgagg
     5881 ccgaaggcgg cgttccgctc gtccaccggc gtggtggcga agacctcccg cacgatggcc
     5941 gtgacgatgg ggaggaccat gagggccagc accacgccgg ccgcgaagat gctgcgcacg
     6001 atgggcggtc cggcgaagag cggcaccacc gtgccgatgg tcgaggcgag tgcgtccatc
     6061 accgggaaga cgacgaccgg gacgaagaag aggacgcccc acaggcccac gacgacgctc
     6121 gggatcatgg ccagcaggtc gacggtcgcg gtcagtggac cgcgcagcca ccgtggcgcc
     6181 acctcgacca ggtacagcgc gatgcccacg gccagcggca cggcgatgag cgtcgcgatc
     6241 aggctggtga cgacggtccc gaacaggaag gccagagcgc cgtagtcacc cgtgacgtcg
     6301 gtccgcgatg cccccgggcg ccagtcggtg ctggtgaaga atcccagtcc ctccttggcg
     6361 aacaccggcc aggactcgag cgtcgtggag atgatcatcc acgccaggat cgcgaggacg
     6421 agcgccccgg aggccgtcac cagccaccgg aacgctcggt cacccagtcg tccgcgcgcg
     6481 atcccccgga ccgccgcggt cgcgggacgt gaggcgatct cagccctcct cgttgatccg
     6541 gtcgacctgc tccagcacgc gagcctgcag ctcgtccggg agtggcgcgt agttcagctc
     6601 ggtcgcggtg gcgtcgccct cggtcagggc ccacgtcagg aagcccttca gcgcgtcggc
     6661 ctgggccggc tccatgccgc aactgtacgc caggatccac gtggcaccgg cgatcgggta
     6721 gccgtcaccg ccgacgccga ggatgttgaa gcggaagtcg tccggcacct cgacggtgtt
     6781 ggcggcctcg ctgaccgaat cggtcgacgg caccacggcg tttccatccg cgttgaccac
     6841 cgcggcgacc gccaggcccg cctcgttggc gaactcgtag ctgacgtagc cgataccgcc
     6901 gggctgctgg gagatggccg ccgccacgcc gtcgttctgg tcgccgccga tgccggtcgg
     6961 ccactcgacc tccgagcccg agcccacctg gtccgcccag tcggggctca cgtcctcgag
     7021 gtacttcgtg aagatcgagg tcgtgccgga gccgtcggat cggtgcaccc aggtcagcgc
     7081 ctggtcgggc aggtcgacgt caggattcag cgacgcgatg gccgggtcgt tgacgttcgt
     7141 gatgtcgccg agcgcgatct tcgcgatcgt ctcgccgtcc agcgtgagct ggtccacgcc
     7201 cggcacgttg aaggcgatgg cgacgccgcc gaagaccgtc gggatgtgga ggacgtcgga
     7261 gcagccgctg gcatcgagcg ccgcggcgat ctcgtcgtcc ttcatgaagg catccgaccc
     7321 ggcgaagtcc acctggcccg cgatgaactg ctcgcgaccg ccggaggagc cgatgccctg
     7381 gtagttgatg gtgacgcccg gctcgacgtc ctggtactgg ccgatccatt ccaggtagat
     7441 cggctccggg aagctggcgc ccgcgccgac gaggtcggcg gagaggccgg tcccgccgcc
     7501 gctcgacgcg ggggagtcgt cggaagtgcc ggtgccgccg ccgcaggcgg cgatggtgag
     7561 ggtcagtccc aggcctgccg cgatgagtgg ccgccgggtg gtgggagtca cggtcgattc
     7621 agtcctctgc atgaaggcac ggaccacatc gtcaccgtgg cgcctcaacg gggcgctagg
     7681 cgaatgtcaa cgttcggtta agggcggcta cccgaacagg ggcttgatca cgaggtacgc
     7741 cagcgcggcc gccaggccgg aggcggggag cgtgaggatc caggcggtga cgatggagcg
     7801 cgccacgccc cagcgcacgg tcttcaggcg gtccgacgag ccgacgccca tgatcgcgga
     7861 gctgatgacg tgcgtcgtgc tgaccggcat gccgaagtgc gacgcgccga agatgacggt
     7921 ggcggccgtc gtctccgcgg cgaagccatg gaccgggtcg agcttcacga ccttggtccc
     7981 catggtcttg atgatccgcc agccgccggc cgcggtgccg agcgagatgg cggaggcggc
     8041 gaccaggatg acccacaggg gcacctcgaa gctggggatg acgccggccg tcacgagggc
     8101 cagggtcatg atgcccatcg tcttctgcgc atcgttgctg ccgtggctga aggccatgta
     8161 ggccgctgag agcacctgga gacggcggaa gaccgcgttg agccgggcgg gatgggcgcg
     8221 gcggaagatg ttgaagatca gcgccatcag cagcaggccg ccgatgaacc cgacgatcgg
     8281 ggagccgacg agcgggacca ggaccttgtt cacgatcccg tccatctgcc acgctcccag
     8341 gccggatgcg gtcagcgcgg cgccgagcag cccgccgatc aacgcgtggc tcgacgacga
     8401 cgggatgccg agccgccagg tcagcaggtt ccaggcgatc gcaccgacga gcgccgccgc
     8461 gatgaccgcc tgcgactcca cctgcgtgtc gatcagcccg gccccgacgg tgtgcgccac
     8521 ggcggtgccc gtcagcgcgc cgacgaagtt ggccacggcg ctcatgacga tggcccagcc
     8581 cggggccaga gcgcgcgtcg agacgctggt cgcgattgcg ttggccgtgt cgtggaagcc
     8641 gttgatgtag tcgaacgcga cggacagcgc caggacgatc agcagcgtga gcgtgatgcc
     8701 ctcaagcatg cttgacgacg atgctctcga tcacgttggc cacgtcctcg caggcgtcga
     8761 tggtgtcctc gaggaggccg tacacgtcct tccacttgat cacgtgcacc gggtcggcgc
     8821 cgttcatgaa caggtccgcg atcgcctgcc gcgcgatgcg gtcgccctcg ttctcgaggc
     8881 ggtgaatctc gatccagtgc ggctcgacgc ccttgaagcc gcggagcttc tccagggccc
     8941 ggaccatctc ctcgcactgc agcaccacga tccgcgcctg ctccgtggcg gtctgggtcg
     9001 gtgcgtcgat cttgtacagg atgcaggtgt ccgcgacctc ctcgatgaag tcgatgacgt
     9061 cgtcgaggcg gctgatgagc tggtggatgt cctcgcggtc gaagggcgtg acgaaggtct
     9121 tcgccagccg ctgaccgatg tcgtggctga tgccgtcgcc ccgacgctcc gtgtcgcgga
     9181 gctggctggc gagccgctcg cgctggtcgt aggagcggaa cagctcctcg agcatgcggg
     9241 cgccggcgag cacgttctgc gcgtcttcga cgaagaggtc gaagaacttc tcctcgcgcg
     9301 ggatgaaggg caggcgcatg ggtggtgagg tccccgtgca tgggtctggg agggcggcgg
     9361 gcaccgccac catctagcgt actccgcgaa ccgctaccat cgcgcgcgat gcgacgcgtg
     9421 ctgctctgga tggcccgcaa caggatgctg cgcgacctgg tgccgcggct ctggttcgcg
     9481 aagcgtgcgg tcggtcgctt catgcccggc gagtcgatgg acgaggcgct ggatgccgcg
     9541 gagcgctatc gccacgacgg catcggcgcc atcttcacgc acctgggcga gaacctggcc
     9601 gacctctcgg aggccgatgc ggtcgccacc cactaccacg agctgctcga ccgcatcgtc
     9661 gcacggggga tcgacgggga gatcagcgtc aagctcaccc agctgggcct ggacctggac
     9721 cgggaggcgt gcgcgcggca cctgggtgcg ctggcggcgc gcgcccggga gatcggcgcg
     9781 gggacggtgt ggatcgacat ggagggctcg gcgtacaccg agacgacggt ggcgctgtac
     9841 gagcagctca agccagagca ccccaacctc gggatctgtc tgcaggcgta cctcaagcgc
     9901 acggcggccg acatcacgcg cctgggaccg ctgcggccgt cgatccggat ggtcaagggc
     9961 gcttacgacg agccggcccg gatcgcgtat cggtctcgat ccgaagtcga tgccagcttc
    10021 gtctcgcagt cggtcgtgat cctgcgtgac ggcgccgagc gcctgggcct cggcacgcac
    10081 gacgtgcgcc tgatcgagca gatcgcgacc tcggccgacg tggcggggat ctcgcgcgac
    10141 gcctatgagg tccagatgct gtacggcatc cggaccgacc agctgcgccg cctgtcgaag
    10201 gccggctacc gggcgcgcgt gctcatcgcc tacggcgagt tctggtatcc gtggtacgtg
    10261 cgccggctgg ccgagcgccc cgcgaacgtc ctcttcgtcc tccgccagct gctcccctgg
    10321 tgacccgcgc ctagccgatg cccgccaggc gggcgagggc ggccgtcgcg gccgcgacca
    10381 cgaccgtgac gagcaccggc gcccgcagcg ccagcgccac caccgccgcg ccgatgccga
    10441 tgagccgcgc atcggccacc agggcctgtc cctgtccgag ggtggcggtc gcgacgagcg
    10501 cggcgagcag tgccggggcc agcagcgaca cgaccgcgtc cacggcccgc ggcaggggtc
    10561 ggccgcccag cagcaccggt ccggcggcct tgatcacgac cgtgcccacg cccacgacca
    10621 gcacggtgag ccaggcgtca ttcatcggcg caccaggccc acgagcgcac cggccgcggc
    10681 cgccacgatc ggcacgcccg gcggggtgaa cggcacgagc accagcgcga tgccgccgcc
    10741 gagcagcgcg gccgtgatcg cccgccggct ccgcacctgg ccggcgagca gggccaggaa
    10801 cagcgccgga aaggcggcgt cgaggccgag ggcggccgga tccccgagcg actcgccgcc
    10861 gacgacgccg atgatcgtcc ccgcgaccca ggccagccag atcgtcatgc cggcaccgac
    10921 cagccggccg gcgtcgaagt gcccgggccc gagcgacgcg atcgcccacg actcgtccac
    10981 cacgagctgc gcgtgcacga agcgggcgag cgccgagccg cggagggctc gagcgatgct
    11041 gatcccgatc ggcaggtagc gcgcgttgag cagcagcgcc gcggcgatgg cggcgatcgg
    11101 ctgtccaccg gtcgtcagga ccgacgcggc cgcgaactgc gcggagccgg cgaaggtggt
    11161 ggcgctcatg acgacggctg ccacgccgcc cagtccgtga ccggaggtgg cgaggacgcc
    11221 gaacgagacg ccgaagagcc cgacggccac ggccagcggc agcgcatcgc gcgcgccgtg
    11281 gcggactccg gggcgcggcg accggtcgct cacgccttcg gcaggctgaa ccagaaggtc
    11341 gtcgtgccgc gctcggagcg ggcgccgacc cgtccaccgg ccatctcgac cagctgcttg
    11401 acgatggcca ggccgatccc ggcgccgccg ctgcgccggt cgcgggaccg atccagccgg
    11461 tagaagcgct cgaagacgcg gccgaggtcg tcggccggga tggtggtgcc ggtgttgagc
    11521 accgagacca gcacgtcgtc gcgctgctcc tcggcgcgga ccgtgaccgt gccaccctcc
    11581 ggcgtgtagc gcacggcgtt ctggagcagg ttggcgagga cctgggccag gtggtccgga
    11641 tcggcgcggg cgtggagcga cgtcgggagg tcgagctgcc accgcaggcc gcggaccgtg
    11701 gccgacggtc gcgccagcgc gacggccgtc tccactgccc gactgacgtc gatctcggtc
    11761 cgcgcggagg ggcgggggcc ctcctcgagc cgatcgagcg actccgagag ccggaccagc
    11821 cgctccgtct cgtcccacag cgactcgaag gtggcgcgat cggccggcat gacgccgtcc
    11881 cgcagcgcct cgaggtagcc cttgaggttc gtcagtggcg tccgcagctc gtgcgccgca
    11941 ttggcgatga actcgcgccg catccgctcc tgctcgagga gccccgtgct catctggttg
    12001 aacgaatcgg tcaggctggc cagctcgtcg aagccgcgcc gatccagctg cgcgccgtag
    12061 tcgccctccg cgatgcgccg cgccgcccca ccgaggtcgc ggagcggctg cgccacccag
    12121 cggccggcgg cgatggcgag ggcggtggcc aggccgatgg ccacgaccag cgccacgatc
    12181 agcaccacca ggaccgtcga ctcgaacatc gagcgggcgt cctccgccgt ctcgccgtgc
    12241 gcggtcatga ggtccatgaa cgcgttgccg ccgaagacga tgacgccgac cgtgacgatg
    12301 gccagcgtga cgagggcgac cgccagcgcg gcaaccgcaa tgcgcacgcc gatgctgccg
    12361 ggcagcctca cggcgtccgg ccctcgtcgg cctcgacggc gcggtagccg acgccgcgca
    12421 cggtggccac gaagcgcggc gcgtcgggat cgtcgctgag cttggcgcgc agccgcttga
    12481 tgtgcgcgtc caccgtgcgg tcgaggacga aggcctcacc gcgcccgtgg accgcgtcca
    12541 ggagctgatc gcgggtgagg acccggccgt cggcgtcgag cagggccagc agcagctcca
    12601 tctcgatcct ggtcagctcg acgggtgccc cgtcgaccgt cacccgccgc cgatcgcggt
    12661 ccacgacgag cgcgccgtgg cggatcggct cgtgcgtctg tgggcggggg tcaggcgtcc
    12721 gcagcagccg gcggatgcgc aggacgagct ctgccgggct gaagggcttg ggcacgtagt
    12781 cgtccgcacc ttcctcgagg ccctcgaccc ggtcacgcgc cgtcccccgc gccgagagga
    12841 cgaccaccgg cgtggcgtcg cggcgccgga tggcgcgcag gacgctgatg ccgtcgacct
    12901 cgggcagcat gaggtcgagc accaccagcg ccggtggatc gagcgccatg gaggcgaggg
    12961 ccgaccgccc atcgtgggcg gccgtgaccc ggtgtccctc gcgctcgagg taggtgcgga
    13021 cgaggtgcac gatcttcgga tcgtcgtcca ccaccaggat cggtcgctcg tcaccaccgg
    13081 tgctcacggc gagcatccta ggcgcccatg cggccgatgg caccacctcg tgccgcctac
    13141 tggccggccg gcacccggtt gacggtgcac acgaacatct tcagcggatc ggtaccgatg
    13201 gtcagcttgc ccatgacggc gtacgcctcc acctgctccg cgctggtcag ctgcaccggc
    13261 gcgacgtgcc attcgatgcc cagcacgatc cagcgaccgc cgttgtagtc gcggtctccg
    13321 ggcttcgcct ccgccacgtt gatgaggccg ttgccgagcg cgtagatgcg gtcgtaggac
    13381 tgttccggcg cgccggtgtc cgacagatcg gtgggcgtgg ccacggtgcg gtaccgctcg
    13441 ccgtccacgt agaaggccag ccccggcggg ccggcggccg ccgccaccgt gctcacggcg
    13501 agggccgtga cgaagacggc cgtcgccatg gccgccacga gtcgtcgcat ggtcagttcc
    13561 tcctggagcg cctggctccg atgtgcggac ggcccggtgc cgccgctgat ggggcgcacc
    13621 atcggccgcg gccgtgaagg gccgtcgtcc gtcccgtgac ggcggtgtga cgcgcgcgtc
    13681 acccctccga cgccgacccc gcccggacgt cccgggtcgc gccggtcggg cggcaccccc
    13741 gcgtgagccg gtcggccctg cccggcgctg ggtgctggcc ggtacaaccc tctgcatgac
    13801 gacgaccgag atggcggtga ccccgccgac ctcccgatgg atcgaggccg agcgagccac
    13861 gctgcgcgag gtcctgatgg gggcacagga caacctcacc aacgtgctgg ccgtggtgct
    13921 gggcgtatcg atcggcgcgg gccgtgccga cctcgtgggg ctggccggcc tgtcggccgc
    13981 cgtcgccgag gcgatctcga tgggcggggt gctctacacc gcgacccgcg cggaagccga
    14041 cctggagccg gatcgcgcct cgcggctgtc gccggtcgcg gcgggcgtgg tgtgcgcggt
    14101 ggctgcgctg ctcggcgggc tcgtgccgct cgcgccgttc ctgctgctgc ccatcggcct
    14161 ggcggtcgtg gcgtcgctgg ccatgtcgtt gctggcgctg ttcgtattgg gcgcgtggag
    14221 cgcgacggtc acacgacgca cctggtggcg cgacggcgcg cgcctggtgc tgatcgccgg
    14281 cgccgcggcg ctggccgccg ccctgatcgg gcacgggctg cgcgtcgact gactgccgcc
    14341 gctcaggcgg cgccgagcac cgagtcgagg aacgcgcgca gccgggcgtt gaacggctcg
    14401 ccggtacgct gaacgctgtg ggcccggccg gtgatgacct cgcgctccga gccgagccgc
    14461 tccgcgatgt gatcgcacac cgcgtcgaac gcggcgtgat gaccgcccga gaagaccagt
    14521 gtcggcatcc ccgctgcggc gacctcgtcc agggggatgg acgcggtcca ggggagcggc
    14581 tcgagacgag aggcgaggat cgaggcctcg gtctccgggg gcagcgtctc cgggaggcgt
    14641 ggtgtcgctc cgacggttgc ggcgaacgcc aggaagaact cgcgcgccgg cagctcgtgg
    14701 tcggcgaaga gcggctcgag tctcgcgatg agctcctcga ccgccggaat cccgcgcacc
    14761 aggccgaacg ccggcggctc gatcacggtg agcgagcgca cgcgatcggg caccagcgcc
    14821 gtggcgagca tggataccac gccaccatac gaatggccga taacgtggct ccccggctcg
    14881 atcagctcgg cgaccgcgcg ggcctgcgcg tcgaactgga tgccctctcc gggtgcgttg
    14941 ggcgggtacc ccggccggtg cggcacgacg agccggtact cctctgccag aacgcgttgg
    15001 gcctcccagg tctgccagcc gggaacgatc ccgccgtgga ccaggagcag ccgcgggccg
    15061 gtctcgccga cgcgctcgac gtacatcg
//
LOCUS       DASTYP010000127         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_847521, whole genome shotgun sequence.
ACCESSION   DASTYP010000127 DASTYP010000000
VERSION     DASTYP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847521"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1030
                     /locus_tag="VFV59_05870"
     CDS             35..1030
                     /locus_tag="VFV59_05870"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU5325393.1"
                     /translation="MPDSPVRVAVTGAAGQIGYALLFRIASGQMFGPDVPVRLQLLEL
                     EAALPALDGVRMELEDCAFPLLDGVVCTSSADEAFGGANWALLVGAVPRKQGMERKDL
                     LSINGGIFTGQGEAIARNAADDCRVLVVGNPCNTNALIGSTAAARHGMPKDRWFAMTM
                     LDENRGRSQLAAKAGVPVREVRELAIWGNHSSTQFPDAWHATIGGRPAADVIGDEAWL
                     QGEFITNVQQRGAEIIRARGQSSAASAANAVIDTVRNITQPTGEIFSSAIAAPAQGGY
                     GVPEGVVFGYPLRATAPGQVEIIQDIEHGDFARAKIEATTNELLEEGSAVSDLLA"
     gene            1027..>2190
                     /locus_tag="VFV59_05875"
     CDS             1027..>2190
                     /locus_tag="VFV59_05875"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEU5325394.1"
                     /translation="MTTDAAARTDVATLCAGDDELELPIVRGTAGDDALDIGKLRSET
                     GLITIDPGYGNTGACTSAITFLDGENGILRYRGYPIDQLAEHSTFLEVAYLLIWGELP
                     TSEQLDRFVSEVTHHTLIREEMRHFFDSFPADAHPMAVLASGTVGMSTFYPDSQNIFD
                     PDAVEITINRLIAKLPTLAAWSFKKAIGQPYVYPRNDLGYAANLLHMMFAVPSEPYEV
                     KPEVAEALDLLLILHADHEQNASTSTVRMVGSTHANLYASISAGISALWGPLHGGANE
                     AVINTLEKIVADGGNIQKWVDKAKDPEDSFRLAGFGHRVYKNFDPRAKIIKRAAERVL
                     SATGADDDLFEIAQRLEEIALRDDYFVSRKLYPNVDFYSGLIYRAIGFPKDMFT"
BASE COUNT          369 a          794 c          708 g          319 t
ORIGIN      
        1 cagccggcag ctcactgtcg agaacaggag accgatgccc gactcccccg ttcgcgtggc
       61 cgtcaccggt gctgccggcc agatcggcta cgcgcttctc ttccgaatcg cctcgggcca
      121 gatgttcggc cccgacgtcc cggttcgcct ccagctcctc gagctcgagg ccgctctccc
      181 cgccctcgac ggcgtccgca tggagctcga ggactgcgcg ttcccgctgc tcgacggcgt
      241 cgtctgcacc tccagcgccg acgaggcgtt cggcggcgcg aactgggccc tcctggtcgg
      301 cgcggtgcca cgcaagcagg gcatggagcg caaggacctg ctcagcatca acggcggcat
      361 cttcacgggg cagggcgagg cgatcgcgcg gaacgcggcc gacgactgcc gcgtgctcgt
      421 ggtcggcaat ccgtgcaaca ccaacgcgct gatcggttct accgcggccg cgcgccacgg
      481 gatgccgaag gaccgatggt tcgccatgac catgctggac gagaaccggg gccgatccca
      541 gctggcggcg aaggccggcg tcccggtccg ggaggtgcgg gagctggcga tctggggcaa
      601 ccactcgtcc acccagttcc ctgacgcgtg gcacgcgacc atcggcgggc ggcctgcggc
      661 cgacgtgatc ggcgacgagg cgtggctgca gggcgagttc atcaccaacg tgcagcagcg
      721 cggcgccgag atcatcaggg cccggggcca gagctccgcg gccagcgccg ccaacgctgt
      781 catcgacacc gtccgcaaca tcacccagcc gaccggcgag atcttcagct ccgcgatcgc
      841 ggcgcctgcc cagggcggct acggtgtgcc cgagggcgtg gtcttcggct accccctgcg
      901 tgccacggcg ccgggccagg tcgagatcat ccaggacatc gagcacggcg acttcgcgcg
      961 ggcgaagatc gaggccacga ccaacgagct cctcgaggag ggctcggccg tctcggacct
     1021 gctggcatga ccaccgacgc tgccgctcgc accgacgtcg cgacgctgtg cgccggcgat
     1081 gacgagctcg agctgcccat cgtccgcggc accgccggcg acgacgcgct ggacatcggg
     1141 aagctgcgca gcgagaccgg gttgatcacc atcgaccccg gctacggcaa caccggcgca
     1201 tgcaccagcg ccatcacctt cctcgatggc gagaacggca ttctgcgcta ccgcggctac
     1261 cccatcgacc agctggccga gcactcgacc ttcctggagg tcgcgtacct gctgatctgg
     1321 ggtgagctgc cgaccagcga gcagctggat cggttcgtct ccgaggtgac gcaccacacg
     1381 ctgatccgcg aggagatgcg gcacttcttc gactccttcc cggccgatgc gcaccccatg
     1441 gcggtccttg cctcgggcac cgtcgggatg agcaccttct acccggactc ccagaacatc
     1501 ttcgatccgg atgcggtcga gatcaccatc aaccgcctga tcgccaagct gcccacgctg
     1561 gcggcgtggt cgttcaagaa ggccatcggg cagccgtacg tctacccgcg caacgacctg
     1621 gggtatgccg cgaacctgct gcacatgatg ttcgcggtgc cgtcggagcc gtacgaggtg
     1681 aagccggagg tcgccgaggc gctcgacctg ctcctcatcc tccacgccga tcacgagcag
     1741 aacgcctcga cctcgacggt gcgcatggtc gggagcacgc acgccaacct gtacgccagc
     1801 atcagcgccg gcatcagcgc cctgtggggc ccgctgcacg gtggcgcgaa cgaggcggtc
     1861 atcaacacgc tcgagaagat cgtggcggat ggcgggaaca tccagaagtg ggtcgacaag
     1921 gcgaaggacc ccgaagacag cttccgcctc gccgggttcg ggcaccgcgt ctacaagaac
     1981 ttcgacccgc gcgccaagat catcaagcgc gccgccgaac gcgtcctgtc ggcgaccggc
     2041 gccgacgacg acctgttcga gatcgcccag cgcctcgagg agatcgcgtt gcgggacgac
     2101 tacttcgtca gccgcaagct ctaccccaac gtcgacttct actccggcct gatctaccgc
     2161 gccatcggct tccccaagga catgttcacc
//
LOCUS       DASTYP010000128         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1649330, whole genome shotgun sequence.
ACCESSION   DASTYP010000128 DASTYP010000000
VERSION     DASTYP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649330"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFV59_05880"
     CDS             <1..748
                     /locus_tag="VFV59_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325395.1"
                     /translation="ALYVSMTFQSLFLQGGLGYTPLAAGAIGLPVAIMLTLLSTPAGQ
                     LAGRWGPRIFMTVGPLIMALGVLWLARIPADSAPWLADLARPDTLLPPADALLDVGIG
                     ISVFGLGLAAMVAPLTTALMSSVPVRNAGLGSAINNAISRAGSPLVFAVIFVAIVSSY
                     YPALASMLPGVDLTDPAVREAVRPFSVGADLEPAVAEAAREAATGSFHLAMFVSAALL
                     AVGAAINGFGIENPSVTRKSPAADPAPGVG"
     gene            complement(791..2011)
                     /locus_tag="VFV59_05885"
     CDS             complement(791..2011)
                     /locus_tag="VFV59_05885"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325396.1"
                     /translation="MDIDVRAFSGDPRGFYDAAEMAFGEWPRDADFDRWKQTFEADRA
                     IAAYDGDAVVGTAGIFTFDMTVPGGVLPAAGVTIVGVQPTHRRRGVLRRMMRLQLDAI
                     HERGEPIAVLWASEGNIYQRFGYGLASLKAAVNVARDRSAFRQPLTPAGSVRFVDLEE
                     AKRRFPPVFDAIRLGRVGFWTRSPEFWTTEFFADPEHWRQGASAAFHVVHEVAGDADG
                     YARYRIRDDWKDGTPKSTVDIVEVLATNPSAYLDLWRYLLDIDLMGQASAWNLAVDDP
                     LLLTIAEPRRLQMTVGDALWLRVVDVASALAGRRYRIDGSVVLEVADEFCEWNDGRWA
                     LRVEDGVPFIGPTTEPADLRCDVSDLGATYLGAFSFAELADAGRVQELQPGGLLRTDA
                     LFRTDRAPWCPRVF"
     gene            2063..>2713
                     /locus_tag="VFV59_05890"
     CDS             2063..>2713
                     /locus_tag="VFV59_05890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5325397.1"
                     /translation="MLPLSDENERGTGPAFISLAFIALNVIVFLLLQAGGAPEGESFT
                     YGFSAVPYEITNNVDLTEPTPITVDGQQAAIPQRPGPDPIWLTVLTSMFMHGGWLHLG
                     GNMLFLWIFGDNVEHRVGHFVYLAFYLAAGVVASLAQIMVSPDSYIPTLGASGAISGV
                     LGAYLVMFPGNRVLVLFLRFPVWVPAIVAIGLWAAFQFINGLGAAAVTAETGGVAYM"
BASE COUNT          369 a          989 c          938 g          417 t
ORIGIN      
        1 ggcgctgtac gtctccatga ccttccagag cctgttcctg cagggcgggt tgggctacac
       61 gccgctcgcc gccggcgcga tcgggctgcc ggtggcgatc atgctcacgc tgctctcgac
      121 ccccgcagga cagctggcgg ggcgctgggg cccgcggatc ttcatgaccg tcgggccgct
      181 gatcatggcg ctgggcgtcc tgtggctggc ccgcatcccg gccgactcgg caccgtggct
      241 cgccgacctg gcgcgaccgg acacgctgct gccgccggcg gacgcgctgc tcgacgtcgg
      301 gatcggcatc agcgtcttcg gcctgggcct ggcggccatg gtcgcgccgc tgacgaccgc
      361 gctgatgagc tcggtgccgg tgcgaaacgc cggactcggc tcggcgatca acaacgccat
      421 cagccgcgcc ggatcaccgc tcgtgttcgc cgtcatcttc gtggcgatcg tgagctcgta
      481 ctacccggcg ctggcctcca tgctccccgg ggtcgacctg acggaccccg ccgtccgcga
      541 ggcggtccgc ccgttctccg tcggcgcgga cctcgagccc gccgtggccg aagcggcgcg
      601 cgaggcggcc accggctcgt tccacctggc catgttcgtg tctgcggccc tgctcgccgt
      661 cggcgcggcg atcaacggct tcgggatcga gaacccctcg gtgacgcgga agagcccggc
      721 cgccgacccg gctcccggcg tcggctgacc acgtcgccgg gcggcgtcgg cacccggcgc
      781 atcggcctcg tcagaagacc cgtgggcacc agggtgcccg gtcggttcgg aagagcgcat
      841 cggtgcggag caggccgccc ggctggagct cctgcacgcg tccggcatcc gccagctcgg
      901 cgaagctgaa cgcccccagg tacgtcgccc ccaggtccga gacgtcacag cgcaggtcgg
      961 ccggctccgt cgttgggccg atgaacggca cgccgtcctc cacgcgcagc gcccagcgac
     1021 cgtcgttcca ctcgcagaac tcgtcggcca cctcgagcac taccgagccg tcgatgcggt
     1081 agcggcggcc ggccagggcc gaggcgacgt cgaccacgcg cagccacagg gcatcgccga
     1141 cggtcatctg cagtcgccgc ggctcggcga tggtgagcag cagcgggtcg tccaccgcga
     1201 ggttccatgc gctggcctgg cccatcaggt cgatgtcgag caggtagcgc cacagatcga
     1261 ggtacgccga cgggttggtg gcgagcacct cgacgatgtc gacggtcgac ttcggggtgc
     1321 cgtccttcca gtcgtcgcgg atgcggtagc gggcgtagcc gtcggcatcg ccggcgacct
     1381 cgtggaccac gtggaacgcg gccgacgcgc cctgccgcca gtgctcggga tcggcgaaga
     1441 actcggtggt ccagaactcc ggtgaacgcg tccagaagcc gacgcgtccg agccggatgg
     1501 cgtcgaagac cggcgggaag cgccgcttgg cctcctccag gtccacgaat cggacgctgc
     1561 ccgccggcgt cagcggctgg cggaacgcgc tccgatcgcg cgccacgttg accgccgcct
     1621 tgaggctcgc cagcccgtag ccgaagcgct gatagatgtt gccctcggat gcccagagca
     1681 cggcgatcgg ctcgccgcgc tcgtggatgg cgtcgagctg gagtcgcatc atccgacgca
     1741 ggacgccacg ccgccgatgc gtcggctgca cgccgacgat ggtcacgccg gcggccggga
     1801 gcacgccgcc gggaacggtc atgtcgaagg tgaagatgcc ggcggtgccg acgaccgcgt
     1861 cgccgtcgta tgcggcaatg gcgcgatccg cctcgaaggt ctgcttccag cggtcgaagt
     1921 cggcatcccg gggccactcg ccgaacgcca tctcggcggc gtcgtagaag ccgcgcgggt
     1981 cgccgctgaa cgcgcgaacg tcgatgtcca tgcgccgatt atggggccgg agctggcgtg
     2041 ggtcgtgctc tagaatccgc cgatgctccc gctctccgac gagaacgagc gcggcaccgg
     2101 gccggcgttc atctccctcg cgttcatcgc gctcaacgtg atcgtcttcc tgctgctgca
     2161 ggcgggaggg gcccccgaag gcgagagctt cacgtacggc ttcagcgcgg tgccgtacga
     2221 gatcaccaac aacgtcgacc tgaccgagcc cacgcccatc accgtcgacg gacagcaggc
     2281 cgcgattccg cagcggcccg gccccgaccc gatctggctc accgtcctga ccagcatgtt
     2341 catgcacggc gggtggctgc acctgggcgg caacatgctg ttcctgtgga tcttcggcga
     2401 caacgtcgag caccgggtcg gccacttcgt ctacctggcc ttctacctgg ccgccggggt
     2461 cgtcgccagc ctcgcccaga tcatggtctc gcccgattcg tacatcccga ccctcggcgc
     2521 ctcgggcgcg atctccgggg tcctgggcgc gtacctggtc atgttccccg gaaaccgcgt
     2581 gctcgtcctc ttcctgcgct tcccggtgtg ggtgcccgcc atcgtggcca tcgggctgtg
     2641 ggccgccttc cagttcatca acggactcgg cgccgcggcg gtgaccgccg agaccggcgg
     2701 cgtggcgtac atg
//
LOCUS       DASTYP010000129        23113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1175356, whole genome shotgun sequence.
ACCESSION   DASTYP010000129 DASTYP010000000
VERSION     DASTYP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 23113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23113
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175356"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFV59_05895"
     CDS             complement(<1..651)
                     /locus_tag="VFV59_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU5325398.1"
                     /translation="MIDRLGELEARYEEIGRELSSPDAAANPSRLADLGRELSRLEPI
                     VADLRAYRAVREEIESTKAMLEDPDEEMRSMARDELERLREREAALESDLRLQLVPRD
                     PNDDRNVIVEIRAGAGGEEASLFAADLYRMYARYAERRRWKVEVMSTSESEAGGFKEI
                     IAEVRGDGAYSRLKFESGVHRVQRVPVTEAQGRIHTSTATVSVLPEADEVEVDIDEK"
     gene            complement(662..1666)
                     /locus_tag="VFV59_05900"
     CDS             complement(662..1666)
                     /locus_tag="VFV59_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEU5325399.1"
                     /translation="MAAGPLVIAAATDANGRRRFRMPDFFYGGQALIEGVMMRGRTTV
                     AMSVRPPSGEIRTMSEPLPAALSADRKLIKIPLLRGIFVLYETLVIGTRMLMRSAAIA
                     AEGEDIELGKGTIAITMVFSLGFAVALFFLLPLFLSTFAHEAADSDFVANAVEGVIRL
                     VIFIGYIAAIGLMQDIRRVFAYHGAEHKAISAYEHERPLTPEEVDAFGTAHTRCGTTF
                     ILIVVVISIFFFSLIPRTGVPLWALFASRIVLVPLIAGVAYELVRFGARHYGNPVVRA
                     IYAPGLWLQSLTTRPPDRSMLEVSIASLESCLASDRAAAGTLAPATADVVDPTTGITP
                     "
     gene            complement(1750..1956)
                     /gene="rpmE"
                     /locus_tag="VFV59_05905"
     CDS             complement(1750..1956)
                     /gene="rpmE"
                     /locus_tag="VFV59_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU5325400.1"
                     /translation="MKADIHPTYHQARVHCACGNEFTVGSTAETIRVEVCSKCHPFFT
                     GTQNIVDTAGQVERFQRRLERSLR"
     gene            complement(1973..2257)
                     /gene="rpmA"
                     /locus_tag="VFV59_05910"
     CDS             complement(1973..2257)
                     /gene="rpmA"
                     /locus_tag="VFV59_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5325401.1"
                     /translation="MAHKKAGSSSKNGRDSAGKRLGVKRGDGQYVLAGTIIVRQRGST
                     FHPGPNVGMGRDYTLFATADGKVRFSHETRSKKRVSIEAGPYQSVMAQSA"
     gene            complement(2261..2773)
                     /gene="rplU"
                     /locus_tag="VFV59_05915"
     CDS             complement(2261..2773)
                     /gene="rplU"
                     /locus_tag="VFV59_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU5325402.1"
                     /translation="MYAVVTTGGKQYRVEPGSELVVERLAADPGATITFDRVLLVGDG
                     EAVTVGTPTVDGATVSATVLGEALGPKLIIFKFKQKVKYRRHTGHRQHLTRVRIDEIT
                     SGGRKATRSEAATEQPAAEAEAPAEKPKRTRKATPKAEAASADAGETAEATEKPKRTR
                     KAATKAESEE"
     gene            complement(2879..3595)
                     /locus_tag="VFV59_05920"
     CDS             complement(2879..3595)
                     /locus_tag="VFV59_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEU5325403.1"
                     /translation="MTNRSPDAPPRQRWQILFSRTEPALRMRQAELVDELERAFREAG
                     LPLAYTHAKKPRPRIKLAAAPPIGIELRGEVVEAYFDDLVPIDRIRAAAELFPEGVEL
                     RDAKEVWHGFPSAASQLRAAEYEVDIRGDESLTDEALRGAVVRLLGAKELTGFRRRGE
                     SERRSDAGDRDLRPLIEDVEVVEVDEETHTGRLRTVLQLDATGAGRPEDVVTALDLPV
                     RPVRSVRTRLLFVDTPPIAR"
     gene            3656..4828
                     /locus_tag="VFV59_05925"
     CDS             3656..4828
                     /locus_tag="VFV59_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5325404.1"
                     /translation="MADFLLAVRRHRQALFALAMIVIVLALLWTARGALPAFFIGLAL
                     AFVLDPAVTWLNRHGVPRWAGVVIMYVAVVVVVWALLAYALPPISNQTREFIRHLPEL
                     GSALGDWERGLARWYADLPLPSELRDMIDQQLAASGQAIGEIVRGILAPTINALVRAA
                     TFILGLVVVPVWLFFVLKDREGFSRSVAGALPPAWRADAENVLALLGRVGGRWVRGQL
                     LLGLSIFLASVVGLTILTFIGFREFGQFTLVLALIAGVLEWFPIIGPIVAAIPAILIG
                     LSIGVPAAIAAAVLYTAIQQLENNLLVPKVMGDAVELHPAVMILALVVGGALFGIGGA
                     ILAAPTVAAGRDLYRYGFHRFGGQIPADALELTLHGTVRTDEPEAPSEPDEVPSPA"
     gene            complement(4875..6008)
                     /locus_tag="VFV59_05930"
     CDS             complement(4875..6008)
                     /locus_tag="VFV59_05930"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5325405.1"
                     /translation="MGVATATFVESVRDRAWRYFDLQLLLYVALLIGVGVVMGYSAGY
                     NAPTGGAMSQTVKTLIWAAIGLTLFFVAASIDYHWLRTLAAPIYLVVLGLLTLTMLVG
                     TNLFGAQMSVTVAGLDFQFSEASKVLMIAVLAAYLAGRRDRIGRLSTILVAGALMALP
                     TLLVFRQPDLGTALVFVAILVGMLFMSGASLGWMGLFAAGVVGAAPLAIGMLQDYQRQ
                     RLFCFLDPYADPQGACFQLVQALNAVGAGGWLGRGLTVGGEGQRGYIPVQSTDFIFTV
                     IAEDLGFIGGVVVLAIFALLVWRILAIGWRAQDAFGMLIAVGLASAILFQILVNVGMV
                     IGIMPVTGIPLPFVTYGGSSLISLLFGMGILESIRMRSHKPTL"
     gene            complement(6021..7934)
                     /gene="mrdA"
                     /locus_tag="VFV59_05935"
     CDS             complement(6021..7934)
                     /gene="mrdA"
                     /locus_tag="VFV59_05935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5325406.1"
                     /translation="MADLNDGRLDLRRSRARFVAFGVAAALLFVVLGGRLFQLQVVSG
                     DTYARRAAADRTAEVPIPAPRGLIFDREGRPLAVNVPSWTVKVRPADLPDEMRNRVLR
                     RVAEIAGVPVAGLRARLDAFGGSPYDLVPVARGITREAALLIGEEAETLPGIVVEVDP
                     VRQYLDETGAANGTLLSHVVGYTGPISAEQLAGLAERGYLPDDPIGRDGVEASFEDEL
                     RGEYGSQLVQRDASGRIAKVIETVREPTPGTNLVLTVDAETQRLATQALQWGMQVAGV
                     SQGVTIVMNPQTGEILAMVSLPAYDNNKFATGISADDYAVYLADPNRPLRNHAISDIY
                     PPGSTYKLVTGIAAMEEGVTTASQRWPTFGCYQIPGAPEGECLYDWNRQGFGPLDMVD
                     AFAKSSDTFFYQMAVNTGIDPLARWAGELGFGQRSGIDLPGEEPGIIASTEWARSQGR
                     AGVFTGEVAQAGIGQNVIAVTPLQVLNAYAAVANGGHLMRPTVVLGEADDQGTLVERY
                     EPEVIHDVAADPATLQTMRIGAREVITSGHAYNIKDLRLPGALSGKTGTAEFGEPTEE
                     GTLPFHSWFVAYLPSTAGATDAELAVVSFNYSAVVPGNVSAEVVKYFLQLYFDLDQDL
                     RLDPNDFSLVTAN"
     gene            complement(7971..8531)
                     /locus_tag="VFV59_05940"
     CDS             complement(7971..8531)
                     /locus_tag="VFV59_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325407.1"
                     /translation="MPALAKSPFVYSRVVTAGGPSSISYVSPLLAIVIGIIHAALAPV
                     IVIGGVKPNLALAAVVLVTTQLGFGAGVTWAFVTGLTVNLLVGEPLGTVPLALLLVAA
                     VVAGGQRLLGAAVWVYPVAAAFAGSLIADAVSIGAAQLVADSPTLVVPGELVLAAATL
                     NAAIVGLLLLPARTLALRFGDEAPSW"
     gene            8667..9428
                     /locus_tag="VFV59_05945"
     CDS             8667..9428
                     /locus_tag="VFV59_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007409899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5325408.1"
                     /translation="MVRVTACTNQKGGVGKTTTVINLAAYLALSGTRTLVIDLDPQGN
                     ATSGLGIDRRRVDRSTYDALVDRAPIAELIRGTSIDGLFLVPSSSALSGAEVELVGVA
                     ARERRLSASLAELDGRFDRVLIDCPPSLGLLTVNALTAADGVLIPIQTEYYALEGLSQ
                     LVNTIRRVREGLNPRLEIEGVLLTMYDARTRLSAQVATEVRRHMNGTVYQTVVPRSVR
                     LSEAPSHGLPIALYDPGSRGATAYRELAGEVAARG"
     gene            9421..10347
                     /locus_tag="VFV59_05950"
     CDS             9421..10347
                     /locus_tag="VFV59_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5325409.1"
                     /translation="MASTPARPGRAFGLGRGLDALIPTGHEERGVLEVPLDRISRNPD
                     QPRLAFDETQLGELAASIAVHGVLQPIVVRALADGEYQLIAGERRLRAARLAGLETIP
                     VIVRESTDRADSLELALIENLQRENLNPIETALAYRELIDRFGLTHEAVARQVGKSRV
                     AVSNSLRLLDLAAETREAIVAGRITEGHGRALAAITIPELQRAVLAVVLERGLSVRQT
                     EELVRRKREERPPPQRGQLSSDLQDLEAQLRGVLATKVGIQRTRRGGRLVIDFYSDEE
                     LDRLYSIITRGAGGAAAAPASVHHDHEDLSPA"
     gene            10344..12320
                     /gene="gyrB"
                     /locus_tag="VFV59_05955"
     CDS             10344..12320
                     /gene="gyrB"
                     /locus_tag="VFV59_05955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU5325410.1"
                     /translation="MTVERAPKSRSKAATSYTAANIQVLEGLQAVRKRPGMYIGSTDA
                     RGLHQLVWEVVDNSIDEAMANQATRIDVTIHADGTCEVVDDGRGIPVDKHSTGKSALE
                     VIMTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSSEMRVEVLRDAKRYVQEFVRGTP
                     KGPVKQTGGASEANRGLEPGWKRTSGTRTFFKPDPEMFETLDFSWDTISTRLRESAYL
                     NKGLWICLKDQRVDAEKNFFFQGGVTSFVRHLNRRRDVLNPRPIYVERMADSTSVEVA
                     LQYNDGFTETILAFANNIHTVDGGTHVAGFRAALTSSLNTWARKSGTLGEKDANLSGD
                     DVREGLTAVISVKLTDPQFEGQTKAKLGNADVKGAVQTIVTEGIVQHLEENPADGRRI
                     IEKCVTAARAREAARKARDLVIRKGALEGMALPGKLADCQERDPARSELYIVEGDSAG
                     GSAKQGRDRRFQAVLPMFGKMLNVEKARLDRVLSSDKIRPLVIALGAGIGDTFDIAKL
                     RYHRICLLSDADVDGAHISTLLLTFFYRHMPEVIENGYLYLCQPPLFRVSTGKVTRYA
                     HTEKERDAALKELSRDGKTKNVSVQRFKGLGEMNADQLWDTTMNPDTRTLLRIEVSDA
                     AEADEIFSTLMGEKVEPRRDFIRGEARKVRNLDI"
     gene            12353..12598
                     /locus_tag="VFV59_05960"
     CDS             12353..12598
                     /locus_tag="VFV59_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEU5325411.1"
                     /translation="MPSRHAADIRQVPQKTGRSLDEWCELLEANGIGERRDAVNFLRD
                     SYAVSHAFALAIANECLRRSDDDWGGGDDFGDGDDPW"
     gene            12601..13113
                     /locus_tag="VFV59_05965"
     CDS             12601..13113
                     /locus_tag="VFV59_05965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5325412.1"
                     /translation="MAPDAEPVGSSREIVAADGRRYAFADHHCFACGGTNPIGMGLHI
                     ELDEGRARTTWRAGDDFVGWSDKLHGGIVATLLDEVMAWACASSDAWAVTAEMTVRYR
                     SPAAPGEELVAEGRVVEQRRRIYQVVGEVRGGDGRLVAEGSGRYLGASPTQKADLKER
                     YGAAPGVAGE"
     gene            13200..15707
                     /gene="gyrA"
                     /locus_tag="VFV59_05970"
     CDS             13200..15707
                     /gene="gyrA"
                     /locus_tag="VFV59_05970"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5325413.1"
                     /translation="MERTGNVRPVAIEDEMRTAYIDYAMSVIVARALPDVRDGLKPVH
                     RRILYTMHEMGLRSTAAYRKCAGVVGEAMAKYHPHGDLALYETLVRMAQDFSLRYPLV
                     DGQGNFGSVDGDPPAAMRYTEARMSAITDEMLADIEKETVDFVDNYDGRLRQPTVLPS
                     KLPNLLVNGSSGIAVGMATNIPPHNLNEVAAAIRALIDNPEISVDELCGIVTGPDFPT
                     GGTIFRYEEQRNALTGEKERIDAIRHMYATGRGRIIIRGQVAFEEIRAGRMAVVVTEL
                     PYQVNKTSLIEKMADLVGAKKITDVSDIRDESDRDGMRIVVEVKRDGSPHTVMQQLFK
                     HTQLQTSFSANMLALVDGQPQTLGLKKMLEHYITYRREVVRRRTEFDLARAQERAHIL
                     EGLKIALDNLDEVIATIRAAADVDAARQALIKKFGLTEVQANAILEMQLRRLAALERK
                     KIEDEYRSIIQLIAELEDLLANPRKILLVIKDELADLEKRYGDDRKTRIQADANRELT
                     AEDLIASEDVVVTLSQRGYIKRQPISTFRSQRRGGRGKLAMLTREEDAVRHLLVANTH
                     DNILFFTNRGRVFITKVHTLPDASRQAKGLPIINLPGVQVEQREYVSAIISLPQFEPD
                     HYLVMATRRGMIKKTSLAEYAKIRANGLIAINLQGGDELQWVGLSDGQSDIIVATKKG
                     QAARFRESEVRPLGRDTMGVAAIRLKGEDEVIGMEVVQNREDELLVVTEQGYGKRTEV
                     EDFPVKHRATGGVIANSLNRETGDVAAIRLVGRDEEELMLITELGTILRTEVGGINRY
                     RRASRGVTVMKPQAGDRIVSITVFVDNRLPEELPEAE"
     gene            15754..16794
                     /locus_tag="VFV59_05975"
     CDS             15754..16794
                     /locus_tag="VFV59_05975"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5325414.1"
                     /translation="MYADEFSIYAGNANRPLAERICRYLQTDLGAVEVFQFANENIFV
                     RVLENVREQDVFLVQPTSRPVNQSIMELLIMIDAFKRASAGRITAVIPYYSYGRSDKK
                     DQPRVPITARLVADMLTVAGADRVLSMDLHQGQIQGFFSIPVDELTAVNLLSAHIRAK
                     QLDDLVVVSELGFAKKARNFAELLQVPLAIVEKRRLGNQDRTEVMNIIGDVRGKNAVI
                     IDDEIDTAGSLMEAVGALQAEGARQIYSCATHGVFSGAALERIAASPLTEVIVTDTIP
                     LPPGADDPRITVLSVAPLFGEAIRRIHRGESVGALFSSEVQLVEEMTFWDEDGGEADD
                     EEDPTPFRVSAR"
     gene            16916..17668
                     /locus_tag="VFV59_05980"
     CDS             16916..17668
                     /locus_tag="VFV59_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003938684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325415.1"
                     /translation="MLRRLLILGATVGGAVAVYRRVVEPWWRTWGVRPDEAGAPLAGD
                     EIIGGPDVTIETRGIDIEAPAEAVWPWLVQMGFGRAGWYSYDQLDMEGRSADRIVPEH
                     QSLAVGDTVPTHPAGGFEVRAIEPRRHLVLYLDDEMVRRQAEEAAAINAESPANLRMA
                     GAFMENAQPPEFSASWAFVLEPRDGGTRLIERFRIRFGSEGTKPWTRLTLPVMGFGVF
                     VMMRKQMLGIRDRAERLRMPAPEPRPTEAATA"
     gene            17716..20598
                     /gene="secA"
                     /locus_tag="VFV59_05985"
     CDS             17716..20598
                     /gene="secA"
                     /locus_tag="VFV59_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5325416.1"
                     /translation="MFKFLSRMVDSNEREVRALQPIVEQINALEPEIQALTDAELASK
                     TDAFRARLRAEIGDILTPIELREIDPDEEAAESTFAGADAARVADDLKRQREQELKQI
                     NAALDEILPEAFACVREAMVRALGKRHYDVQLMGGVVLHRGAIAEQRTGEGKTFVAPL
                     AAYLNALVGRGVHVVTVNDYLAKRDSQWIGAVFHRLGMSVGSIQHDGAYLFDPDYPAT
                     DERLRDLRPASRREAYAADVTYGTNNEFGFDYLRDNLVVDLGSRAQRGHFFGIVDEVD
                     NILIDEARTPLIISGAAEESADKYIQFARLVPRLKAEEDYIVDEKFKQVAITEAGTSK
                     MEGWLGVENMFADDFSLARHLEQALKAEVLYKRDRDYVVKDGEVIIVDEFTGRLMPGR
                     RWSEGLHQAVEAKEGVAVRSEQRTLATVTFQNYFRMYDKLAGMTGTAETEAEEFAKIY
                     GLEVVVIPTHRDMIRADYADLIYATQKAKWNAVVDEIVEEHEKGRPVLVGTISVEISE
                     MLADLLKRRGIKHNVLNAKFHEKEAEIIAQAGRSGAVTIATNMAGRGTDILLGGNPEV
                     LAAELLHKRGTNVLEASKEEYDAALREAEAICAEDKEKVLEAGGLHIVGTERHEARRI
                     DNQLRGRSGRQGDPGSSRFYLSLEDDLMRRFASDRVQSIMRTLGFTEDTALESKMVSR
                     TIENAQSRVEGYNFDTRKHVVEYDDVINRQRETIYRERDRILRSSDLGPTILAMLEDE
                     VAALVAEHTAAEHFDDWNLDGLKAQLTTMIPTLTAAELRVVDEARDAETVTQSLMDLV
                     RDRYERKRSETGDEGMKVLERLVLLRVIDSLWVEHLTAVDDMRRGIGLRAYSQREPLN
                     EFKIEAYKMFDELKSIIRHDVTHTIFRVTVSAQPSQPAAASPRRTVEGRAPVAGATST
                     ASGAATATAVAAGNGNQQPARSGPKVGRNDPCWCGSGKKFKKCHGA"
     gene            complement(20648..20809)
                     /locus_tag="VFV59_05990"
     CDS             complement(20648..20809)
                     /locus_tag="VFV59_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325417.1"
                     /translation="MPDPFFLVLGLIAILCVILFPIRLWQRRRHRYGNEEADAGEVPL
                     APPERRNRG"
     gene            20944..21237
                     /locus_tag="VFV59_05995"
     CDS             20944..21237
                     /locus_tag="VFV59_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325418.1"
                     /translation="MKQLTAPVRASVPLPVSTTAHLPPVTRFRDPDLPYEDLIMCERD
                     CMRCSYAATLNCDGNCGVCPRQNYCPCVNPAKVRTKQSRLDMELPMVAGVGRN"
     gene            21224..22349
                     /gene="prfB"
                     /locus_tag="VFV59_06000"
     CDS             join(21224..21259,21261..22349)
                     /gene="prfB"
                     /locus_tag="VFV59_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010886623.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU5325419.1"
                     /translation="MDEIRDEAQRLAASLTTRRCVFDLAAKEAELAELTERSADPNLW
                     NDPAEAQSIMRRAEGLRAEIDAWRDLGDRARGLAEMAEMAAADVEEGETLLPDLQRDL
                     ASLQADWGRMEQQLLLSEPFDERGAIVSVHAGAGGTESQDWAEMLLRMYLRWAERNRF
                     KTEILEATEGEEAGLKSVSFSIDGRWAYGKLRSERGVHRLVRISPFDSQKRRHTSFAL
                     VEVMPEVPEDAAVEIEDKDLRVDTYRASGAGGQHVNKTDSAVRLTHLPTGIVVAVQSE
                     RSQHQNKDRAMAVLRAKLIERQEEEREAELARLRGEHIEAGWGNQIRSYVLQPYTMVK
                     DLRTGVETSNPTAVLDGDLDAFIEGFLRSRVGEGEPTPAA"
     gene            22461..>23113
                     /gene="ftsE"
                     /locus_tag="VFV59_06005"
     CDS             22461..>23113
                     /gene="ftsE"
                     /locus_tag="VFV59_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU5325420.1"
                     /translation="MVDVGMTYPNGKVALADVTVRIPAGDFVFLVGPSGAGKSTFIRL
                     LIREQLPTHGHVRVAGRDLLRLRRGQVPGLRRRIGIVFQDFRLLPNKTVWENVAFALE
                     VTGSPGARIRGRVPQLLSLVGLQDHAHHLPTQLSGGEQQRTAIARALVHDPAILIADE
                     PTGNLDPVTSWEIIQLLIQINQLGTTVLMATHNQEIVNAMRRRVLALENGSLVRDEAA
                     "
BASE COUNT         3788 a         7995 c         8079 g         3251 t
ORIGIN      
        1 cttctcgtcg atgtcgacct ccacctcgtc cgcctccggc aggaccgata cggtcgccgt
       61 cgaggtgtga atccggccct gcgcctcggt caccggcacg cgctggacgc ggtgcacgcc
      121 cgactcgaac ttgaggcgcg agtaggcccc gtcgccgcgc acctcggcga tgatctcctt
      181 gaagccgcca gcctccgact ccgaggtcga catgacctcg accttccagc gccgccgctc
      241 ggcgtaccgc gcgtacatcc ggtacaggtc ggcggcgaac agcgacgcct cctcgccacc
      301 ggcaccggcc cggatctcga cgatgacgtt gcggtcatcg ttcgggtcac gcggcacgag
      361 ctgcagccgc aggtcggact ccagcgccgc ctcgcgctcg cgcagccgct ccagctcgtc
      421 gcgtgccatg gaccgcatct cctcgtccgg atcctcgagc atcgccttcg tcgactcgat
      481 ctcctcgcgc accgctcggt acgcgcgcag gtcggcgacg atcggctcca gccgcgagag
      541 ctcacggccc aggtccgcca gccgcgacgg gttggccgcg gcgtccgggc tgctgagctc
      601 tcggccgatc tcctcgtacc gcgcctcgag ctcgcccagc cggtcgatca tggtgccgtc
      661 ctcagggcgt gatgccggtg gtcgggtcca ccacgtccgc cgtcgcgggg gccagcgtgc
      721 cggccgcggc gcgatcggac gcgaggcacg actccaggct ggcgatgctc acctcgagca
      781 tcgagcggtc cggtggccgc gtggtcagcg actggagcca caacccgggc gcgtagatgg
      841 cgcggacgac cgggttgccg tagtggcggg cgccgaaccg caccagctcg tacgcgacgc
      901 cggcgatcag cggaacgagg acgatgcggc tggcgaacag cgcccacagc ggcacgccgg
      961 tccgcgggat caggctgaag aagaagatgc tgatgaccac cacgatcagg atgaacgtcg
     1021 tcccgcatcg ggtgtgcgcg gtcccgaagg cgtcgacctc ctcgggcgtc agcggccgct
     1081 cgtgctcgta cgcgctgatg gccttgtgct ccgccccgtg gtaggcgaag acgcgccgga
     1141 tgtcctgcat caggccgatg gcggcgatgt agccgatgaa gatcaccagc cggatcacgc
     1201 cctcgacggc gttcgccacg aagtccgaat cggcggcctc gtgggcgaag gtcgagagga
     1261 agagcggcag caggaagaac agcgccacgg cgaacccgag gctgaagacc atggtgatcg
     1321 cgatcgtccc cttgccgagc tcgatgtcct cgccctcggc ggcgatcgcc gcggagcgca
     1381 tcagcatccg cgtcccgatg accagggtct cgtacagcac gaagatcccg cgcaggagcg
     1441 ggatcttgat cagcttccga tcggcggaga gggccgccgg gagcggctcc gacatggtgc
     1501 ggatctcacc ggacgggggt cggacgctca tggcgaccgt cgtgcggccg cgcatcatga
     1561 cgccctcgat gagcgcctgc ccgccgtaga agaagtccgg catgcggaat cgccgccggc
     1621 cgttggcatc ggtcgcggcg gcgatcacca ggggcccggc tgccatcagc agctcgggga
     1681 tgaggaagaa caggtggggc gccaggggcg cccgcaaccc ggtcgggcgc gcggcgcgcg
     1741 gtgcccgggt cagcgcagcg agcgctccag gcgccgctgg aaccgctcga cctggccggc
     1801 ggtgtcgacg atgttctgcg tgcccgtgaa gaacgggtgg cacttgctgc acacctcgac
     1861 gcggatcgtc tccgcggtcg acccgaccgt gaactcgttg ccgcaggcgc agtggacccg
     1921 cgcctggtgg taggtgggat ggatatcggc cttcatgatg gtgctccgcg cgtcaggcgg
     1981 actgggccat taccgactgg tatgggccag cctcgatgct gacgcgcttc ttgctgcggg
     2041 tttcgtgact gaagcggacc ttgccgtccg cggtggcgaa gagcgtgtag tccctgccca
     2101 tcccgacgtt cgggcccgga tggaaggtgc tgccgcgctg gcgcacgatg atcgtgccgg
     2161 ccagcacgta ctggccgtcg ccgcgcttca cgcccaggcg cttgccggcg ctgtcgcgtc
     2221 cgttcttgct ggacgagccg gccttcttgt gtgccatcga ttactcctcc gactcggcct
     2281 tggtcgcggc cttgcgggtg cgcttcggct tctccgtcgc ctccgccgtc tccccagcat
     2341 cagccgatgc ggcctccgcc ttcggcgtcg ccttgcgggt gcgcttcggc ttctcggccg
     2401 gggcctccgc ctcggcagcg ggctgctccg tcgctgcctc ggacctcgtg gccttccgac
     2461 cccccgaggt gatctcgtcg atccggacgc gggtcaggtg ctggcgatgg ccggtgtggc
     2521 gtcggtactt caccttctgc ttgaacttga agatgatgag cttcggcccg agggcctcgc
     2581 cgaggacggt ggcgctgacg gtggcgccgt ccacggtcgg cgtgccgacg gtgaccgcct
     2641 cgccgtcgcc gacgagcaga acgcgatcga acgtgatcgt ggcaccggga tcggccgcga
     2701 ggcgctcgac gacgagctcg ctccccggct ccacgcggta ctgcttcccg ccggtcgtga
     2761 cgacggcgta catgcttgct ctcctgggtc tgcctgatga ggtctcgccg cgtcgcggca
     2821 gaagcatgcg acgcgcacga agaacgggcc gagtgctcag cccgcgggac cgagaagtct
     2881 accgagcgat cgggggagtg tcaacgaaga ggaggcgggt ccggacggag cggacgggcc
     2941 ggacgggaag gtccagcgca gtcaccacgt cctccggccg gccggcgccg gtggcgtcca
     3001 gctgaagcac ggtccgcagc cgcccggtgt gcgtctcctc gtcgacctcg accacctcga
     3061 cgtcctcgat cagcggccgc aggtcgcgat ctcccgcgtc gctgcggcgc tcgctttccc
     3121 cgcgccgtcg gaagccggtc agctccttcg cgcccagcaa cctgacgacc gcgccgcgca
     3181 gcgcctcgtc ggtcagtgac tcgtcgccgc ggatgtcgac ctcgtactcc gcggcgcgca
     3241 actgggaggc ggccgacgga aagccgtgcc acacctcctt ggcgtcgcgc agctccacgc
     3301 cctccgggaa cagctccgcg gccgcgcgga tgcggtcgat gggcaccagg tcgtcgaagt
     3361 acgcctcgac cacctcgccg cgcagctcga tcccgatcgg cggagccgcg gcgagcttga
     3421 tccgcgggcg cggcttcttg gcgtgcgtgt aggccagcgg caggccggcc tcgcggaacg
     3481 cgcgctccag ctcatcgacg agctcggcct ggcgcatccg gagcgccggc tcggtgcggc
     3541 tgaagaggat ctgccaccgc tgacgcggcg gcgcatcggg ggaacggttg gtcatgcggg
     3601 ccgaatcgta ccgcccgggc cctcgcgcgg ccagcgcggc gctaggatct gcgcgatggc
     3661 cgacttcctc ctcgccgtgc gtcgccaccg ccaggcactc ttcgcgctgg cgatgatcgt
     3721 catcgtcctc gcgctgctgt ggacggcgcg tggcgcgctg ccggcgttct tcatcggcct
     3781 ggcgctggcc ttcgtgctcg atccggcggt cacgtggctc aaccgtcacg gcgttccgcg
     3841 ctgggccggc gtggtgatca tgtacgtcgc ggtggtggtg gtggtctggg cgctgctggc
     3901 gtacgccctg ccgcccatca gcaaccagac gcgcgagttc atccgccacc tgccggagct
     3961 cgggtcggcg ctgggtgact gggagcgcgg actggcgcgc tggtacgccg acctgccgct
     4021 gcccagcgag ctgcgcgaca tgatcgatca gcagctggcc gcgagcgggc aggccatcgg
     4081 cgagatcgtg cgcggcatcc tcgcaccgac gatcaacgcc ctggtcaggg cggcgacctt
     4141 catcctcggg ctcgtcgtcg tgccggtctg gctgttcttc gtgctgaagg accgcgaggg
     4201 cttcagccga tcggtcgccg gcgcactccc cccggcgtgg cgagcggacg cggagaacgt
     4261 cctcgccctc ctcggtcgcg tcggcggccg ctgggtgcgc ggccagctgc tcctcggcct
     4321 ctcgatcttc ctcgcctccg tggtgggcct gaccatcctc accttcatcg gcttccggga
     4381 gttcgggcag ttcaccctgg tgctggcgct gattgccggt gttctggagt ggttcccgat
     4441 catcggtccg atcgtggcgg cgatcccggc catcctgatc ggcctctcca tcggcgtgcc
     4501 ggcggccatc gccgccgccg tgctgtacac cgccatccag cagctggaga acaacctgct
     4561 ggtgccgaag gtgatgggcg acgcggtgga gctgcacccg gcggtgatga tcctggccct
     4621 ggtggtcggt ggcgcgctgt tcggcatcgg cggcgcgatc ctcgcggccc cgacggtcgc
     4681 cgccgggcgc gacctgtacc gctacggctt ccaccgattc ggcggccaga tcccggccga
     4741 tgcgctggag ctcaccctcc acggcacggt tcgcacggac gagcccgaag ccccgtcgga
     4801 gcccgatgag gtcccctcgc cggcctgatg cagcaccctg gtgcgggcgc ccgacgagcc
     4861 ggacgctctg gaattcaaag cgtcggcttg tggctccgca tgcggatgga ctcgaggatg
     4921 cccatcccga acagcaggct gatcagcgac gacccgccgt aggtcacgaa ggggagggga
     4981 atgcccgtca ccggcatgat gccgatgacc atcccgacgt tcaccaggat ctggaagagg
     5041 atcgcgctgg ccagtccgac cgcgatgagc atcccgaacg cgtcctgcgc gcgccagccg
     5101 atggccagga tccgccatac cagcagcgcg aagatcgcga ggaccaccac gccgccgatg
     5161 aagccgaggt cctcggcgat gacggtgaag atgaagtccg tcgactgcac cgggatgtag
     5221 ccgcgctggc cttcgccgcc cacggtgagc ccgcgaccga gccagccacc ggcacccacg
     5281 gcattcagcg cctggaccaa ctggaagcag gcgccctgcg gatcggcgta gggatccagg
     5341 aagcagaaga ggcgctgacg ctggtagtcc tgcagcatgc cgatggcgag cggggccgcc
     5401 ccgaccacgc ccgcggcgaa gagccccatc caccccaggc tcgcgccgct catgaagagc
     5461 atgcccacca ggatcgcgac gaacacgagc gcggtcccga ggtccggctg ccggaacacg
     5521 agcagcgtcg gcaacgccat gagcgcgccg gccaccagga tcgtcgagag gcgaccgatg
     5581 cgatcgcgtc gcccggcgag gtaggcggcg agcaccgcga tcatcagcac cttgctggcc
     5641 tcgctgaact ggaagtcgag gccggcgacg gtgacggaca tctgggcgcc gaacagattc
     5701 gtgccgacca gcatggtcag ggtcagcagc ccgaggacga ccaggtagat cggcgccgcg
     5761 agggtgcgca gccagtggta gtcgatgctc gcggcgacga agaacagggt gaggccgatg
     5821 gccgcccaga tcagggtctt gacggtctgg ctcatcgccc cgccggtcgg cgcgttgtac
     5881 cccgcggagt agcccatcac gaccccgacg ccgatgagca gggcgacgta gagcagcagc
     5941 tggaggtcga agtaccgcca ggcgcggtca cggaccgatt cgacgaaggt tgcggtcgcc
     6001 acacccatcg gtcagccctc tcagttcgcc gtcacgaggc tgaagtcgtt ggggtccagg
     6061 cggaggtcct ggtcgaggtc gaagtacagc tggaggaagt acttcacgac ctcggccgag
     6121 acgttgcccg ggaccacggc cgagtagttg aagctcacga ccgccagctc ggcgtccgtg
     6181 gcgccggccg tgctgggcag gtaggcgacg aaccagctgt ggaacggcag cgtcccctcc
     6241 tcggtcggct caccgaactc ggcggtgccc gtcttgcccg acagcgcgcc cggcagccgc
     6301 aggtccttga tgttgtaggc gtgtcccgac gtgatcacct cgcgcgcgcc tatgcgcatg
     6361 gtctgcagcg tggccgggtc ggcggcgacg tcgtggatga cctccggctc gtagcgctcg
     6421 accagggtgc cctggtcatc ggcctcgccc aggacgaccg tcggccgcat caggtgaccc
     6481 ccgttggcga cggcggcgta ggcattcagc acctgcaacg gcgtcaccgc aatgacgttc
     6541 tgaccgatgc cggcctgcgc cacctcgccg gtgaacaccc cggcgcgccc ctgcgagcgc
     6601 gcccactcgg tgctggcgat gatccccggc tcctcgccgg gaaggtcgat gcccgaccgc
     6661 tggccgaacc ccagctcgcc cgcccagcga gcgagcggat cgatgccggt attgacggcc
     6721 atctggtaga agaaggtgtc ggagctcttg gcgaaggcat cgaccatgtc gagcgggccg
     6781 aagccctggc ggttccagtc gtagaggcac tcgccctccg gggctccggg gatctggtag
     6841 cagccgaagg tcggccagcg ctgcgacgcc gtcgtgacgc cttcctccat ggccgcgatg
     6901 cccgtgacca gcttgtaggt ggagcctggc gggtagatgt cactgatggc gtggttgcgc
     6961 aggggacggt tcggatcggc gaggtagacg gcgtagtcgt ccgcgctgat gccggtggcg
     7021 aacttgttgt tgtcgtaggc ggggagcgac accatggcca ggatctcgcc ggtctgcgga
     7081 ttcatgacga tcgtcacgcc ctggctgacg ccggccacct gcatgcccca ctggagcgcc
     7141 tgggtcgcca ggcgctgggt ctcggcgtcg acggtgagaa cgaggttcgt cccgggtgtc
     7201 ggttcccgca ccgtctcgat gaccttcgcg atccgtccgg atgcgtcgcg ctgcaccagc
     7261 tgcgagccgt actcgccgcg cagctcgtcc tcgaaggagg cctcgacgcc atccctgccg
     7321 atggggtcgt ccgggaggta gccgcgttcg gcgagacccg ccagctgctc cgccgagatt
     7381 ggtccggtgt agccgacgac gtgcgagagc aaggtcccgt tcgccgcgcc ggtctcgtcc
     7441 aggtactgcc gcaccgggtc gacctcgacg acgatgcccg gcagcgtctc agcctcctcg
     7501 ccgatgagca gcgcggcttc ccgcgtgatg ccgcgcgcca ccggaaccag gtcgtacggg
     7561 gacccgccga aggcgtcgag gcgagcccgc agcccggcca ccgggacgcc ggcgatctcc
     7621 gcgacgcggc gcagcacgcg gttcctcatc tcatcgggca gatcagccgg acgaaccttg
     7681 accgtccacg aggggacgtt gaccgcgagc gggcgtccct cgcggtcgaa gatcagtccg
     7741 cgcggcgccg ggatcggcac ctcggcagtc cggtcggcgg ccgcgcgcct ggcgtaggtg
     7801 tcgccgctga cgacctgcag ctggaacagc ctgccgccga gcaccacgaa aagcagcgcg
     7861 gctgcgacgc cgaaggccac gaaccgagcg cgtgaccgcc gcaggtcgag ccggccgtcg
     7921 ttcaggtccg ccatcggtca gtcacgcatc gcgcccgggc atggccaggg tcaccaactc
     7981 ggtgcctcgt cgccgaaccg cagggcgagg gtgcgagcgg ggagcagcag caggccgacg
     8041 atcgccgcgt tcagggtggc cgctgccagg acgagctccc cgggcacgac cagcgtcggg
     8101 gagtcggcca cgagctgtgc cgcgccgatg gacacggcgt cggcgatgag tgagccggcg
     8161 aacgcggcgg caacgggata cacccagacg gccgcgccga ggaggcgttg cccacccgcc
     8221 acgaccgcgg cgacgagcag cagggcgagc gggaccgtcc ccagcggctc gccgacgagc
     8281 aggttgaccg tgagacccgt gacgaaggcc cacgtcacgc cggcgccgaa tccgagctgc
     8341 gtggtgacca gcacgacggc cgccagggcg aggttcggct tcacgccgcc gatgacgatc
     8401 acaggggcca gcgcggcgtg gatgatgccg atgacgattg cgaggagcgg gctgacgtag
     8461 gagatggaag acgggccccc tgcagtgact acccgcgagt atacgaaggg cgacttcgcc
     8521 agcgccggca tgcttcacgt gaaacatctg tggacaactt cctggcgcgg tccctcgcga
     8581 gggcgccggt aagccgttgg taagcacgga gacggggctc aactggtaaa atgtgcatgc
     8641 ccatctgaac tcgcagcgga gtgctcgtgg tccgggtcac ggcctgcaca aaccagaagg
     8701 gcggcgtggg aaagaccacc acggtgatca acctggccgc ctacctcgcc ctctccggca
     8761 cccgaacact cgtcatcgac ctcgatcccc aggggaatgc gacgagcggc ctcgggatcg
     8821 accggcgtcg ggtcgaccgt tcgacgtacg atgcgctggt cgaccgcgcg ccgatcgcgg
     8881 agctgatccg ggggacttcg atcgacggcc tgttcctggt tccgtcctca tcggcgctct
     8941 cgggtgccga ggtcgagctc gtcggcgtag ccgcccgtga acggcggttg tcggcgagcc
     9001 tggccgagct cgacgggcgg ttcgatcggg tgctgatcga ctgtcccccg tcgctggggc
     9061 tgctgaccgt caacgcgctg acggcggcgg acggtgtgct gatcccgatt cagacggagt
     9121 actacgccct ggagggcctg agccagctgg tgaacacgat ccgacgggtc cgcgaaggcc
     9181 tcaacccgcg cctcgagatc gagggcgtgc tgctcaccat gtacgacgcg aggacgaggc
     9241 tctcggcaca ggtggccacc gaggtgcggc ggcacatgaa tggcaccgtc taccagacgg
     9301 tggtgcctcg ctcggtgcgg ctgtccgagg cacccagcca cgggctgccg atcgcccttt
     9361 acgacccggg ctcccgtggc gcgaccgcgt accgcgagct cgccggcgag gtcgccgccc
     9421 gtggctagca ctcccgcacg tccggggcgc gccttcgggc tcggccgcgg tctcgatgcc
     9481 ctcatcccca ccgggcacga ggagcgcggc gtgctcgagg tgccgctcga ccgcatcagc
     9541 cggaatcccg atcagccgcg cctggcgttc gacgagacgc agctcggcga gctggcggcg
     9601 tcgatcgcgg tccacggggt gcttcagccc atcgtcgtcc gggccctggc cgacggcgag
     9661 taccagctga tcgccgggga gcgccggctc cgggcggcgc gcctggcggg cctcgagacc
     9721 atccccgtca tcgtgcgcga gagcaccgac cgggcggatt cactcgagct ggcgctcatc
     9781 gagaacctgc agcgcgagaa cctcaacccg atcgagaccg ccctcgccta tcgcgagctg
     9841 atcgaccgct tcgggctcac ccacgaggcg gtggcccggc aggtcggcaa gagccgggtc
     9901 gcggtcagca actcgctgcg cctgctcgac ctggcagccg agacgcgtga ggcgatcgtc
     9961 gcgggccgga tcaccgaggg ccatggccgc gccctggcgg cgatcaccat ccccgagctg
    10021 cagcgcgcag ttctcgcggt cgtgctcgag cgcggcctct cggtgcgcca gacggaggag
    10081 ctggtgcgcc gcaagcgcga ggagcggccg ccgccccagc gcggacagct gagctcggat
    10141 ctccaggacc tggaggcgca gctgcgcggc gtgcttgcga cgaaggtcgg catccagcgc
    10201 acccggcgcg gcgggcgcct cgtcatcgac ttctactccg acgaggagct cgaccgcctc
    10261 tactcgatca tcacccgcgg cgcgggcggc gcggccgccg cgccggcatc ggtccatcac
    10321 gaccacgagg atctgtcacc cgcatgaccg tcgaacgagc cccgaagtcg cgcagcaagg
    10381 cggcgacgag ctataccgcc gccaacatcc aggtcctcga gggcctgcag gcggtgcgca
    10441 agcgccccgg gatgtacatc ggctcgaccg acgcgcgggg cctgcaccag ctcgtctggg
    10501 aggtggtcga caacagcatc gacgaggcga tggccaacca ggccacccga atcgacgtca
    10561 ccatccatgc cgacggcacc tgcgaggtcg tggacgacgg ccgcggcatc cccgtcgaca
    10621 agcactcgac cggcaagagc gccctcgagg tgatcatgac cgtgctccac gccggcggga
    10681 agttcggcgg gggcggctac aaggtgtccg ggggcctgca cggcgtcggc gtcagcgtcg
    10741 tcaacgcgct cagctccgag atgcgggtcg aggtgctgcg cgacgcgaag cgctacgtcc
    10801 aggagttcgt gcgcggcacg cccaagggcc ccgtgaagca gacgggtggg gcatcggagg
    10861 ccaaccgcgg gctcgagccc ggctggaagc gcacgagcgg cacgcgcacc ttcttcaagc
    10921 cggatccgga gatgttcgag acgctcgact tcagctggga cacgatcagc acccggctgc
    10981 gtgaatcggc ctatctgaac aagggactgt ggatctgcct gaaggaccag cgcgtcgacg
    11041 cggagaagaa cttcttcttc cagggcggcg tcacgagctt cgttcggcac ctcaaccggc
    11101 gtcgcgacgt gctcaacccg cgcccgatct acgttgagcg catggccgat tcgacctcgg
    11161 tcgaggtcgc gctccagtac aacgacggct tcacggagac gatcctcgcc ttcgccaaca
    11221 acatccacac cgtcgacggc ggcacgcacg tcgccggctt ccgggccgcc ctgacgagct
    11281 cgctcaacac ctgggcccgc aagtcgggaa cgctgggaga gaaggacgcg aacctctccg
    11341 gtgacgacgt tcgggaaggg ctgacggcgg tcatcagcgt caagctcacc gacccgcagt
    11401 tcgagggcca gaccaaggcc aagctcggca acgcggacgt caagggcgcc gtgcagacca
    11461 tcgtgaccga gggcatcgtc cagcacctcg aggagaaccc ggcggacggc cggcggatca
    11521 tcgagaagtg cgtcaccgcg gcgcgtgccc gggaggccgc gcgcaaggcc cgcgacctgg
    11581 tcatccgcaa gggggccctc gagggcatgg cgctgcccgg caagctcgcc gactgccagg
    11641 agcgggatcc cgcgcgctcg gagctgtaca tcgtcgaggg cgactcggcc ggcggatcgg
    11701 ccaaacaggg gcgcgatcgg cgcttccagg ccgtcctgcc gatgttcggc aagatgctga
    11761 acgtcgagaa ggcccggctc gaccgggtgc tgtcgagtga caagatccgg ccgctggtca
    11821 tcgccctcgg cgccggcatc ggcgacacct tcgacatcgc caagctgcgc taccaccgca
    11881 tctgcctcct cagcgacgcc gatgtcgacg gcgcgcacat cagcaccctc ctcctcacct
    11941 tcttctaccg ccacatgccg gaggtgatcg agaacgggta cctgtacctg tgtcagccgc
    12001 cgctgttccg ggtgagcacc ggcaaggtga cgcgctacgc gcataccgag aaggagcgcg
    12061 atgccgccct caaggagctg tcgcgcgacg gcaagaccaa gaacgtcagc gtccagcgat
    12121 tcaagggcct cggggagatg aacgcggacc agctctggga tacgaccatg aacccggaca
    12181 cccgaacgct cctcaggatc gaggtcagcg acgccgccga ggccgatgag atcttcagca
    12241 cgctgatggg cgagaaggtc gagccccggc gcgacttcat ccgcggggag gcccgcaagg
    12301 tccgcaacct ggacatctga gtcggaacgc tcacggagag gcaaccacgg agatgccatc
    12361 acgacacgcc gccgatatcc ggcaggtgcc ccagaagacc ggtcgcagcc tggacgagtg
    12421 gtgcgagctg ctcgaggcga acggcatcgg ggagcgccgg gacgccgtca acttcctgcg
    12481 tgactcgtac gccgtcagcc acgccttcgc actggccatc gccaacgagt gcctccgccg
    12541 ctccgacgac gactggggcg gtggcgacga cttcggcgac ggggacgacc cctggtagcc
    12601 atggcgcctg acgcggagcc cgtcggcagc agccgcgaga tcgtcgcggc cgatggccgg
    12661 cgctacgcgt ttgcggacca ccactgcttc gcgtgcggcg gcaccaaccc gatcgggatg
    12721 ggcctgcaca tcgagctcga cgagggccgg gcgaggacga cgtggcgagc cggcgacgac
    12781 ttcgtgggct ggagcgacaa gctgcatggg ggcatcgtgg ccaccctgct ggacgaggtc
    12841 atggcgtggg cctgcgccag cagcgacgcc tgggccgtga cggccgagat gaccgtgcgc
    12901 taccgctctc cggcggcacc cggtgaggag ctggtggccg aaggcagggt ggtggaacag
    12961 cgtcgccgga tctaccaggt ggttggcgag gttcggggcg gcgacgggcg cctggtggcc
    13021 gagggcagcg ggcgctacct gggggcatcc ccgacgcaga aggccgacct gaaggagcgc
    13081 tacggcgcgg caccgggcgt cgccggcgaa tgaatgcgac aaacgcgaca atgcgactga
    13141 tggcgcggga atatctctcg agatgctccg gcgcagcgaa gaggtaggag tcgatggaga
    13201 tggaacggac cggcaacgtc cggccggtgg cgatcgagga cgagatgcgc accgcgtaca
    13261 tcgattacgc gatgagcgtc atcgtggctc gcgccctgcc cgacgtgcgc gacggcctga
    13321 agccggtgca tcggcgaatc ctctacacca tgcacgagat ggggctgcgg tcgaccgcgg
    13381 cctaccgcaa gtgcgccggc gtcgtcggcg aggcgatggc caagtaccat ccgcacggcg
    13441 acctggcgct gtacgagacc ctcgtgcgga tggcgcagga cttcagcctt cgctacccgc
    13501 tggtcgacgg ccagggcaac ttcgggtcgg tcgacggcga tccgccggcg gccatgcgct
    13561 acaccgaggc gcggatgtcg gcgatcaccg acgagatgct cgccgacatc gagaaggaga
    13621 ccgtcgactt cgtcgacaac tacgacggac gcctccgcca gcccaccgtc ctcccctcca
    13681 agctgccgaa cctgctggtc aacggctcgt cgggcatcgc ggtgggcatg gccaccaaca
    13741 tcccgccgca caacctgaac gaggtcgccg cggccatccg ggcgctgatc gacaacccgg
    13801 agatcagcgt ggacgagctc tgcgggatcg tgacggggcc ggacttcccg accggcggaa
    13861 cgatctttcg ctacgaggag cagcgcaacg cgctgaccgg tgagaaggag cggatcgacg
    13921 cgatccgcca catgtacgcg acgggacgcg gccgcatcat catccgcggg caggtcgcct
    13981 tcgaggaaat ccgagccggg cgcatggcgg tggtggtcac cgagctgccg taccaggtga
    14041 acaagacgtc gctgatcgag aagatggcgg acctggtcgg cgccaagaag atcaccgacg
    14101 tgagcgacat tcgcgacgaa agcgacaggg acggcatgcg gatcgtggtc gaggtgaagc
    14161 gcgacggctc accgcacacc gtcatgcagc agctgttcaa gcacacgcag ctgcagacca
    14221 gcttcagcgc caacatgctg gcgctcgtcg acgggcagcc gcagacgctc ggcctcaaga
    14281 agatgctcga gcactacatc acctaccggc gcgaggtggt ccggcgtcgg accgagttcg
    14341 acctggcgcg cgcccaggag cgggcgcaca tcctcgaggg cctcaagatc gccctcgaca
    14401 acctggacga ggtcatcgcg accattcgcg cagcggccga tgtcgacgcg gctcgccagg
    14461 ccctcatcaa gaagttcggc ctcaccgagg tgcaggccaa cgccatcctc gagatgcagc
    14521 tgcggcggct cgccgccctc gagcgcaaga agatcgagga cgagtaccgc agcatcatcc
    14581 agctcatcgc ggagctcgag gatctgctgg ccaacccgcg caagatcctg ctggtgatca
    14641 aggacgagct ggcggacctg gagaagcgct acggcgacga tcgcaagacc cgcatccagg
    14701 ccgacgcgaa tcgcgagctc accgcggagg acctcatcgc gtcggaggac gtggtggtca
    14761 cgctcagcca gcgtgggtac atcaagcgcc agccgatcag caccttccgc tcccagcgcc
    14821 ggggcggccg cgggaagctg gcgatgctca cgcgcgagga ggacgcggtg cgccacctgc
    14881 tggtggcgaa cacgcacgac aacatcctct tcttcaccaa ccgcggtcgc gtcttcatca
    14941 ccaaggtcca cacgctgccc gatgcctccc gccaggccaa gggcctcccg atcatcaacc
    15001 tccccggcgt ccaggtcgag cagcgtgagt acgtcagcgc gatcatcagc ctgccgcagt
    15061 tcgagccgga ccactacctg gtgatggcga cgcgccgggg gatgatcaag aagacctcgc
    15121 tcgccgagta cgccaagatc cgggccaacg ggctgatcgc catcaacctg cagggcggcg
    15181 acgagctgca gtgggtcggc ctttcggacg gccagagcga catcatcgtc gccacgaaga
    15241 aggggcaggc ggcgcgcttc cgcgagtcgg aggttcgccc gctgggccgc gacaccatgg
    15301 gcgtcgccgc catccggctc aagggcgagg acgaggtcat cggcatggaa gtcgtccaga
    15361 accgcgagga cgagctgctg gtcgtcaccg agcaggggta cggcaagcgg accgaggtcg
    15421 aggacttccc cgtcaagcac cgggccaccg gcggcgtgat cgcaaactcg ctgaacaggg
    15481 agaccggcga cgtcgccgcc atccggctgg tgggtcgcga cgaggaggag ctgatgctca
    15541 tcaccgagct cgggaccatc ctgaggaccg aggtcggcgg catcaaccga taccgccgcg
    15601 cgtcgcgcgg cgtgacggtc atgaagccgc aggccggcga ccggatcgtg tcgatcacgg
    15661 tgttcgtgga caaccggctt cccgaggagc tccccgaggc cgagtagtcg gtagaatcgt
    15721 gcagcctgac ctgtagcgcc gcaggagacc agcgtgtacg ccgacgagtt cagcatctac
    15781 gccggcaacg cgaaccgtcc gctcgccgag cgcatctgcc gctacctgca gaccgatctc
    15841 ggtgccgtcg aggtgttcca gttcgccaac gagaacatct tcgtgcgggt gctggagaac
    15901 gtgcgcgagc aggacgtgtt cctggtgcag ccgaccagcc gcccggtgaa ccagtcgatc
    15961 atggagctgc tgatcatgat cgacgccttc aagcgcgcca gcgccgggcg gatcacggcg
    16021 gtgatcccgt actacagcta cggccgctcc gacaagaagg accagccgcg ggtcccgatc
    16081 accgcgcggc tggtggccga catgctcacc gtcgccgggg ccgaccgcgt cctgagcatg
    16141 gacctgcacc aggggcagat ccagggcttc ttcagcatcc cggtggacga gctgaccgcg
    16201 gtcaacctgc tctccgcgca catacgggcg aagcagctcg acgacctggt ggtcgtctcc
    16261 gagctgggat tcgcgaagaa ggcgcgcaac ttcgccgagc tgctgcaggt gccgttggcc
    16321 atcgtcgaga agcggcgcct cggcaaccag gaccgcaccg aggtgatgaa catcatcggc
    16381 gacgtgcgcg gcaagaacgc ggtgatcatc gacgacgaga tcgacacggc gggctcgctg
    16441 atggaggcgg tcggagcgct ccaggcggag ggggcgcgcc agatctacag ctgcgcgacc
    16501 cacggcgtct tctcgggggc cgcgctcgag cggatcgcgg cgagcccgct gaccgaggtc
    16561 atcgtgaccg acaccatccc gctcccgccc ggcgccgacg acccgcgcat caccgtgctc
    16621 agcgtcgcgc cgctgttcgg cgaggcgatt cgtcgcatcc accgcggcga gagcgtcggt
    16681 gcgctgttca gctcggaggt ccagctggtc gaggagatga ccttctggga cgaggacggc
    16741 ggcgaggccg acgacgaaga ggatccgacg ccctttcggg tgagcgcccg ctgacctcgc
    16801 cgccatcccg cgccgtgccg ttccgccctc gatcaggtaa ccgacggccc tgcgcgcacg
    16861 gccccggtcg cgcgaggctt ggacgcacag aagcgaacga gcggaggcca ttcccatgct
    16921 gcgccggctg ttgatcctgg gcgccacggt cggcggcgcc gtggcggtgt atcgacgcgt
    16981 cgtcgagccg tggtggcgga cctggggagt gcgtcccgac gaggccggcg cgccgctcgc
    17041 cggggacgag atcatcggcg gcccggatgt cacgatcgag acgcggggga tcgacatcga
    17101 ggcgccggcc gaggcggtgt ggccgtggct ggtgcagatg ggcttcggtc gggccggctg
    17161 gtacagctac gaccagctcg acatggaggg ccgcagcgcc gatcggatcg tcccggagca
    17221 ccagtcgctg gcggtgggcg acaccgtccc gacgcacccg gccggcggct tcgaggttcg
    17281 cgccatcgaa ccccgccggc acctggtcct gtacctcgac gacgagatgg tgcgcaggca
    17341 ggccgaggag gccgcggcca tcaacgccga gtcgcccgcg aacctgcgca tggccggcgc
    17401 gttcatggag aacgcgcaac cgcccgagtt ctcagccagc tgggcgttcg tcctcgagcc
    17461 acgtgacggc ggcacccgcc tcatcgagcg gttccgcatc cgcttcggca gcgaggggac
    17521 gaagccgtgg acacggctca cgttgccggt catggggttc ggcgtcttcg tcatgatgcg
    17581 caagcagatg ctcggcatcc gtgaccgcgc cgagcgcctg cggatgccgg cgccggagcc
    17641 ccgaccgacg gaggcggcca ccgcctgacc tcgaaggagg gcttgcggac gcatcggtct
    17701 atactcggcc tcccgatgtt caagttcctg tcccggatgg tggactcgaa cgagcgggag
    17761 gtccgggcac tccagccgat cgtcgagcag atcaacgcgc tcgagccgga gatccaggcc
    17821 ctcaccgacg ccgagctcgc ctccaagacc gatgcgttcc gcgcgcggct gcgcgcggag
    17881 atcggcgaca tcctcacgcc gatcgagctg cgcgagatcg atccggacga ggaggccgcc
    17941 gagtcgacct tcgcgggagc cgatgccgcg cgcgtggcgg acgacctgaa gcgccagcgc
    18001 gagcaggagc tgaagcagat caacgccgcc ctcgacgaga tcctgcccga ggccttcgcc
    18061 tgcgtgcggg aggcgatggt gcgcgccctc ggcaagcgcc attacgacgt gcagctgatg
    18121 ggcggcgtgg tgctgcaccg aggcgccatc gcggagcagc gcaccggcga gggcaagacc
    18181 ttcgtggcgc cgctggccgc ctacctgaac gcgctggtcg gacgcggtgt gcacgtcgtg
    18241 acggtcaacg actacctggc caagcgcgac tcgcagtgga tcggcgcggt cttccatcgg
    18301 ctggggatga gcgtcggctc catccagcac gacggtgcct acctgttcga cccggactac
    18361 cccgcgacgg acgagcgact gcgcgacctg cgaccggcct ctcgacgcga ggcgtacgcc
    18421 gcggacgtca cctacggcac gaacaacgag ttcggcttcg actacctgcg cgacaacctg
    18481 gtggtcgacc tcgggtcgcg cgcgcagcgg ggccacttct tcgggatcgt cgacgaggtc
    18541 gacaacatcc tcatcgacga ggcgcggacg ccgctcatca tctcgggcgc ggccgaggag
    18601 tcggcggaca agtacatcca gttcgcgcgc ctggtcccac ggctgaaggc cgaagaggac
    18661 tacatcgtcg acgagaagtt caagcaggtc gcgatcaccg aggcgggcac ctcgaagatg
    18721 gagggctggc tcggggtcga gaacatgttc gccgacgact tcagcctcgc ccgccacctc
    18781 gagcaggccc tcaaggccga ggtcctctac aagcgtgacc gtgactacgt cgtcaaggac
    18841 ggcgaggtca tcatcgtcga cgagttcacg gggcggctga tgcccggccg ccgctggtcc
    18901 gagggtctgc accaggcggt cgaggccaag gagggcgtcg ccgtccgcag cgagcagcgc
    18961 acgctggcca ccgtgacctt ccagaactac ttccgcatgt acgacaagct cgccggcatg
    19021 acgggcacgg ccgagacgga ggcggaggag ttcgccaaga tctacgggct cgaggtggtg
    19081 gtcatcccga cccaccgcga catgatccga gccgactacg ccgatctgat ctacgccacc
    19141 cagaaggcga agtggaacgc ggtcgtcgac gagatcgtcg aggagcacga gaaggggcgg
    19201 ccggtcctcg tcggcaccat cagcgtcgag atcagcgaga tgctggccga cctcctgaag
    19261 cggcgcggca tcaagcacaa cgtgctcaac gccaagttcc acgagaagga ggcggagatc
    19321 atcgcgcagg ccggtcggtc cggcgcggtg accatcgcca cgaacatggc cgggcgcggc
    19381 accgacatcc tcctcggcgg caatcccgag gtcctggcgg ccgagctgct gcacaagcgc
    19441 ggcaccaacg tgctcgaggc ctcgaaggag gagtacgacg ccgcgctgcg cgaggcggag
    19501 gcgatctgcg cggaggacaa ggagaaggtc ctggaggccg gcggcctgca catcgtcggc
    19561 accgagcgac acgaggcgcg gcgcatcgac aaccagctcc gcggtcggtc gggacggcag
    19621 ggcgacccgg gcagcagccg cttctacctc agcctcgagg acgacctgat gcgccggttc
    19681 gcctcggacc gcgtgcagtc gatcatgcgc acgctcggat tcaccgagga caccgcgctc
    19741 gagtcgaaga tggtgagccg caccatcgag aacgcgcagt cccgcgtcga gggatacaac
    19801 ttcgacacgc gcaagcacgt ggtcgagtac gacgacgtca tcaaccgcca gcgcgagacg
    19861 atctaccgcg agcgcgaccg catcctccgg tcctccgacc tcggcccgac catcctggcc
    19921 atgctcgagg acgaggtggc ggcgctggtg gcggagcaca ccgcggccga gcacttcgac
    19981 gactggaacc tggacgggct caaggcccag ctgacgacca tgatcccgac cctgacggcc
    20041 gcggagctgc gcgtcgtcga cgaggcgcgg gacgccgaga ccgtgacgca gtcgctgatg
    20101 gacctggtcc gcgaccgata cgagcgcaag cgctccgaga ccggcgacga gggaatgaag
    20161 gtcctggagc ggctcgtcct gctgcgcgtc atcgactcgc tgtgggtcga gcacctcacc
    20221 gcggtcgacg acatgcgccg cggcatcggc ctgcgggcct actcgcagcg ggagccgctg
    20281 aacgagttca agatcgaggc gtacaagatg ttcgacgagc tcaagtcgat catccgccac
    20341 gacgtgacgc acaccatctt ccgcgtcacg gtgtccgccc agccttcgca gccggcggcc
    20401 gcctcgccgc gtcgcacggt cgagggccgt gcgcccgtgg ccggggcgac ctcgacggca
    20461 tcgggtgccg cgacggccac ggcggtcgcg gccggcaacg gcaaccagca gccggcccgc
    20521 tcgggcccca aggtcgggcg caacgacccc tgctggtgcg gatcgggcaa gaagttcaag
    20581 aagtgccacg gcgcctgagg cgcgcgtaga cctgaccgat gcgcgccacc ggcgcgccgt
    20641 cgtccgccta gccccggttt cgccgctccg gcggcgcgag gggcacctcg cccgcatcgg
    20701 cctcctcgtt cccgtagcgg tgccgccggc gctgccagag gcggatgggg aacaggatca
    20761 cgcacaggat ggcgatgagg cccaggacga ggaagaacgg gtcgggcatg gccgtcatgc
    20821 ttgcacgccg aggaccatcg tgcagggttg cgctctcgga ttcactcgct acgatggacg
    20881 gcaggcacgc gatcgcggaa cggctcgggt ttcgcggcga acgatccgaa ggtggaggga
    20941 ttcgtgaagc agctgaccgc acctgtaagg gcctccgtcc cgctccccgt cagcacgacg
    21001 gcccaccttc caccggtcac ccgcttccga gatccagacc tcccctacga agacctcatc
    21061 atgtgcgagc gtgactgcat gcgctgcagc tacgccgcga ccctgaactg cgacgggaac
    21121 tgcggcgtct gcccgcgcca gaactactgc ccctgcgtca acccggccaa ggtccgcacc
    21181 aagcagagcc ggctcgacat ggagctcccc atggtggcgg gcgttggacg aaattaggga
    21241 cgaggcgcag cggctcgcgc gcctcgttga cgacacgtcg gtgcgtcttt gacctcgccg
    21301 cgaaggaggc cgagctcgcg gagctgaccg agcgctccgc cgatcccaac ctgtggaacg
    21361 accccgccga ggcgcagtcg atcatgcgcc gcgcggaagg cctgcgcgcc gagatcgacg
    21421 cgtggcgtga cctcggcgac cgggcacgcg gcctggccga gatggcggag atggccgcgg
    21481 ccgatgtcga ggagggggag acgctcctgc cggacctgca gcgcgacctc gcctcgctcc
    21541 aggccgattg gggccggatg gagcagcagc tgctcctctc cgaaccgttc gacgaacgcg
    21601 gcgcgatcgt ctccgtccat gccggcgcgg gcggcaccga gagccaggac tgggccgaga
    21661 tgctgctgcg catgtacctg cgctgggccg agcgcaaccg cttcaagacc gagatcctcg
    21721 aggcgaccga gggcgaggag gccgggctga agagcgtcag cttcagcatc gacggtcgct
    21781 gggcctacgg gaagctgcgg agcgagcgtg gcgtgcatcg gctggtgcga atcagcccgt
    21841 tcgactcgca gaagcgacgg cacaccagct tcgcgctggt cgaggtcatg ccggaggtgc
    21901 ccgaggacgc ggcggtcgag atcgaggaca aggacctgcg cgtcgacacg taccgggcgt
    21961 cgggggccgg cggccagcac gtcaacaaga ccgacagcgc ggtgcgcctc acccacctgc
    22021 cgacgggcat cgtcgtggcc gtccagagcg agcgcagcca gcaccagaac aaggatcgcg
    22081 ccatggccgt cctgcgcgcc aagctcatcg agcgccagga ggaggagcgc gaggcggagc
    22141 tggcgcgcct tcgcggcgag cacatcgagg cgggctgggg caaccagatc cgctcgtacg
    22201 tcctccagcc ctacaccatg gtcaaggacc tgcgcaccgg cgtggagacc agcaacccga
    22261 ccgccgtgct ggacggcgac ctggacgcgt tcatcgaggg cttcctgcgc tcgcgcgtcg
    22321 gcgaggggga gccgacaccg gcggcctgac ccgggcgtct gaccgggggt ctccgcgttc
    22381 gctaccatcc ctcgcaaccg atgacagccc tcctgcgccg acgggcgcga cccgccgacg
    22441 ccgacgcccc ggtcatcgag atggtggacg tcggcatgac ctacccgaac gggaaggtcg
    22501 ccctggccga cgtcaccgtc cggatcccgg ccggggactt cgtcttcctg gttggaccgt
    22561 cgggcgcggg caagagcacg ttcatccggc tcctgatccg cgagcagctg ccgacccacg
    22621 gccacgtgcg cgtggccggc cgcgacctgc tgcgcctgcg tcgcgggcag gttcccggac
    22681 tgcggcgccg gatcgggatc gtgtttcagg acttccgcct gctgcccaac aagaccgtct
    22741 gggagaacgt ggccttcgcg ctggaggtga ccggctcacc cggcgcgcgg atccgcggcc
    22801 gcgtgccgca gctgctcagc ctggtcggtc tccaggacca cgcccaccac ctgccgaccc
    22861 agctgtcggg cggggagcag cagcggaccg ccatcgcgcg ggcgctggtg cacgacccgg
    22921 ccatcctgat cgccgacgag ccgaccggga acctggaccc ggtcaccagc tgggagatca
    22981 tccagctcct catccagatc aaccagctcg gcacgaccgt gctgatggcc acgcacaacc
    23041 aggagatcgt gaacgccatg cggcgccgcg tgctggcgct cgagaacggc tccctggtcc
    23101 gcgacgaggc cgc
//
LOCUS       DASTYP010000130         5403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_738543, whole genome shotgun sequence.
ACCESSION   DASTYP010000130 DASTYP010000000
VERSION     DASTYP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738543"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="truB"
                     /locus_tag="VFV59_06010"
     CDS             complement(<1..696)
                     /gene="truB"
                     /locus_tag="VFV59_06010"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5325421.1"
                     /translation="MLGVVNLDKPVGPTSHDMVGLLRRLTGTRRIGHAGTLDPLASGV
                     LPILVGAATRFSEELTGGPKRYDATIRLGARSLTDDAEGPVEPTGASIPGDEEIRAAV
                     ADFVGTFDQRPPAFSARKHAGRTAHRAARAGEPLDLPPRTVTIEAIEVLGIERADGSA
                     DVRVDLRCGAGTYVRSVARDVGERLGCGGYLAALRRTEAAGLRADDGVTPRALEELAA
                     DGRLSAAILPVGDL"
     gene            complement(680..1663)
                     /locus_tag="VFV59_06015"
     CDS             complement(680..1663)
                     /locus_tag="VFV59_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU5325422.1"
                     /translation="MSRDAVLDALRSASRITTICHENPDADSLGSAIALRIAAERMGK
                     QAEVVAADGVPPFLAGLPRADEVRRLPELEPDLAVMLDGPLSRSGAVVAEAGEWLSRA
                     RVVNIDHHVSNDGAEVPLAYVDPDAAATCEIVALMIPDLGVEVDTEIATALAAGIVQD
                     THTFSHPNATPRTLRVMADLVAAGAPLSRLHKAIYADKPFSTMALWGRMLAGIGQRRD
                     GRIVHAAQTLDMLRETGADPTATEGFVDLLASTKAGEITVLFKEAAPDRIRVSVRTAS
                     AADAVAITTAFGGGGHARAAGCTLDVPLSEARGLLLDECERELDRADARGR"
     gene            complement(1653..2009)
                     /gene="rbfA"
                     /locus_tag="VFV59_06020"
     CDS             complement(1653..2009)
                     /gene="rbfA"
                     /locus_tag="VFV59_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5325423.1"
                     /translation="MSQRTDRLDSQIRAELMQLLQREMKDPRVGFATITRVETARDLG
                     SARVWVSVMGSPEEKAATIQALTDAAPWLRRQLGQRLTVRQVPQLVIRADDSIEAGDR
                     VLKLLNELEEERAGEP"
     gene            complement(2017..3843)
                     /gene="infB"
                     /locus_tag="VFV59_06025"
     CDS             complement(2017..3843)
                     /gene="infB"
                     /locus_tag="VFV59_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5325424.1"
                     /translation="MLTEAAPQRPSGPVELPNPVVVKDLSEALGVSNAEIINVLIRNG
                     IFATVNQSIDFDTASLVAGELGMETVEAGAAERAAAEEAREQDLVKPGAESADGRPSF
                     WSEDDPSTLVTRAPIVTVMGHVDHGKTSLLDAVRETKVAAGESGGITQHIGASEVTHN
                     GRRIVFLDTPGHEAFSAMRARGAQVTDIAIIVVAADDGVMPQTKEAIDHVRAARVPMI
                     VAINKIDKPDANPDRVKQELADNNVLIEEYGGDVISVAVSAKQRTGIDELMEMVLLVA
                     DLQDLKANPDREAIGTIVESKVDKGRGNVATVLVQTGTLKVGQVVTVGKTWGRVRALN
                     NAAGKRVKQAEPATAVEIIGLQGLPEAGDHLRVVADEKAARDAAEAAERAAAGGEGRQ
                     VSSLEEMSAAIQASETAELRVVLKTDVQGSLGAIRGALERLNTTEVRINILREGAGDI
                     NESDVNLASASDAVIIGFNTRLDAGAQRAVEATGVDVRVYDVIYKLTDDMAQALQGML
                     APKMVEQVEGHAEVRMVIKAGKAGNIAGSYVTDGRIVRGGQARLFRGSTLLIDTRITS
                     LKRFKDDVREVQTGYECGIGLDYDDIAEGDVIECYQMVSEQA"
     gene            complement(3905..4210)
                     /locus_tag="VFV59_06030"
     CDS             complement(3905..4210)
                     /locus_tag="VFV59_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU5325425.1"
                     /translation="MAPMPAPRRRTMPKRRPDHVPTRTCAVCRTSRPKREMDRLVRTA
                     DGLIHLDPTGKLAGRGTYICHEPACRDPGRAAAAVRRALDAELAPGTLEVEVKNAAE"
     gene            complement(4158..5285)
                     /gene="nusA"
                     /locus_tag="VFV59_06035"
     CDS             complement(4158..5285)
                     /gene="nusA"
                     /locus_tag="VFV59_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU5325426.1"
                     /translation="MNRDFIGALLQLNAEKGVPQEILIRTLEQAIESAYRRNADAPEN
                     VVVTVNPESGEITVAREYEVVAGEQEIEDPELQMTAAAAQKIDPATGVGGKVRVPENV
                     TQAQLGRIAAQTAGQVYRQRLREAQRDVIFQQYAAREGEIMTGNVQRTTDSAVVLDMG
                     KDVEAVLAVTEQLPNETYRIGQHLKVYVMEVRRTTRGPVIVASRTHRGFLRRLMEMEI
                     PEIYNGTVVIRGIAREAGSRSKVAVESRQPGVDAKGAAVGQRGARIQAIVAELNGEKV
                     DIVLWDEDPGRFVAEALSPAEVLSVRIDEEHKIANVIVPERQLSLAIGKEGQNARLAA
                     KLTGWRIDIRSEAGVATAAAGSGEHGADAGTEAPDDAEATT"
BASE COUNT          668 a         1981 c         1865 g          889 t
ORIGIN      
        1 caggtcgccg acgggcagga tggccgcgga gaggcggccg tcggccgcca gctcctccag
       61 cgctcgtggc gtgaccccat cgtcggcccg cagaccggcc gcctccgtcc ggcgcagagc
      121 ggccaggtag ccgccgcaac cgaggcgctc gccgacgtcg cgggccaccg agcgcacgta
      181 ggtgccggct ccgcagcgca ggtccacccg gacgtcggca ctcccgtcgg cgcgctcgat
      241 gccgagcacc tcgatggcct cgatcgtcac cgttcgcggc ggcagatcga gcggttcgcc
      301 ggcccgcgcg gcgcgatgcg cggtccgccc ggcgtgcttt cgcgcgctga aggccggcgg
      361 tcgctgatcg aaggtcccga cgaagtcggc gacggccgcc cggatctcct cgtcgccagg
      421 gatggatgcc ccggtgggct ccaccggtcc ctcggcgtca tcggtcaggg atcgggcgcc
      481 caggcggatc gtcgcgtcgt agcgctttgg gccgcccgtc agctcctcgc tgaacctggt
      541 ggctgcaccg accaggatcg gcagcacgcc ggaggcgagc gggtcgagcg tgccggcatg
      601 gccgatgcgt cgggtcccgg tcagccgccg caggagcccg accatgtcgt ggctcgtcgg
      661 cccgaccggc ttgtcgaggt taacgacccc gagcatccgc gcggtcgagc tcgcgctcgc
      721 actcgtcgag gagcagcccg cgcgcctcgg acagcggcac gtcgagggtg cagccggcgg
      781 ctcgcgcatg gccgccgcca ccgaaggcgg tggtgatggc gaccgcatcg gccgccgagg
      841 cggtgcggac gctgacgcgg atccggtccg gcgccgcctc cttgaacagg acggtgatct
      901 cgccggcctt ggtgctggcc agcaggtcca cgaagccctc cgtggccgtc ggatcggctc
      961 ccgtctcgcg cagcatgtcc agggtctggg ccgcatgcac gatccgtccg tcgcgccgct
     1021 gaccgatccc cgccagcatc cggccccaca acgccatggt gctgaacggc ttgtcggcgt
     1081 agatcgcctt gtggagccgg gagagcggcg ctccggcggc gacgaggtcc gccatcacgc
     1141 gaagggtgcg aggcgtggcg ttggggtgcg agaacgtatg cgtgtcctgg acgatccccg
     1201 cggccagggc cgtggcgatc tcggtgtcca cctcgacgcc caggtccggg atcatgagcg
     1261 ccacgatctc gcaggtcgcg gccgcgtcgg ggtcgacgta cgccagcggc acctccgcgc
     1321 cgtcgttcga gacgtggtga tcgatgttca ccacccgggc gcgggaaagc cactcccccg
     1381 cctccgccac cacggcaccg gagcgcgaca gcggaccgtc gagcatcacc gcgaggtccg
     1441 gctcgagctc ggggaggcgc cggacctcgt ccgcccgcgg caggccggcc aggaagggcg
     1501 gcacgccgtc ggcggccacc acctcggcct gcttgcccat ccgctccgcc gcgatccgga
     1561 gcgcgatggc cgatccgagc gagtcggcgt ccgggttctc gtggcagatg gtcgtgatgc
     1621 ggctggccga gcgcagggcg tcgagcaccg cgtcacggct caccggcacg ctcctcctcg
     1681 agctcgttga gcagcttcag cacgcggtcc ccggcctcga tggagtcgtc ggcacggatg
     1741 accagctgcg gaacctgccg caccgtcagc cgctgcccga gctgccgacg cagccacggg
     1801 gcggcatcgg tcagcgcctg gatcgtggct gccttctcct ctggcgagcc catgacgctg
     1861 acccagacgc gcgcggatcc gagatcgcgg gcggtctcca cgcgcgtgat ggtcgcgaac
     1921 cccacgcgcg ggtccttcat ctcgcgctgg aggagctgca tcagctccgc ccggatctgg
     1981 ctgtcgagcc ggtcggtgcg ctggctcatc ggtccgtcag gcctgctcgg acaccatctg
     2041 gtagcactcg atgacgtcgc cctcggcgat gtcgtcgtag tcgagcccga tgccgcactc
     2101 gtaccccgtc tgcacctcgc ggacgtcgtc cttgaagcgc ttcagcgagg tgatgcgcgt
     2161 gtcgatcagc agcgtgctgc cgcggaagag ccgcgcctgc ccgccgcgca cgatccggcc
     2221 gtcggtcacg tacgagcccg cgatgttccc ggccttgccg gccttgatca ccatccggac
     2281 ctccgcgtgg ccctcgacct gctcgaccat cttcggcgcg agcatgccct ggagcgcctg
     2341 ggccatgtca tcggtcagct tgtagatcac gtcgtagacg cggacgtcca ccccggtggc
     2401 ctcgaccgcg cgctgcgcgc cggcatccag ccgggtgttg aagccgatga tcaccgcgtc
     2461 cgacgccgag gcgaggttga cgtccgactc gttgatgtcg ccggcgccct cacgcaggat
     2521 gttgatccgc acctcggtgg tgttgagccg ctccagcgcc ccccggatgg cgcccagcga
     2581 gccctgcacg tccgtcttca ggacgacccg cagctcagcg gtctcgctgg cctggatggc
     2641 cgcgctcatc tcctccaggc tgctgacctg gcgaccctcg ccgccggccg cggctcgctc
     2701 ggccgcctcg gctgcgtcgc gtgccgcctt ctcgtccgcc accacccgca ggtgatcgcc
     2761 ggcctcgggc aggccctgca ggccgatgat ctcgaccgcg gtggccggct cggcctgctt
     2821 gacgcgcttg ccggcggcgt tgttcagggc gcgcacgcgg ccccacgtct tgccgacggt
     2881 gacgacctgg ccgaccttca gcgtgccggt ctggaccagg acggtcgcga cgttgccgcg
     2941 acccttgtcg accttcgact cgacgatcgt gccgatcgcc tcacggtccg ggttggcctt
     3001 caggtcctgc aggtcggcca ccagcagcac catctccatc agctcgtcga tgccggtgcg
     3061 ctgcttggcg ctcaccgcga cggaaatgac gtcgccgccg tactcttcga tgaggacgtt
     3121 gttgtcggcc agctcctgct tcacccgatc cgggttggcg tccggcttgt cgatcttgtt
     3181 gatggccacg atcatcggca cgcgcgcggc gcggacgtga tcgatggcct ccttggtctg
     3241 cggcatcacg ccgtcgtcgg cggccaccac gatgatggcg atgtcggtga cctgggcgcc
     3301 gcgcgcccgc atggcgctga acgcctcgtg gccgggtgtg tcgaggaaga cgatccggcg
     3361 tccgttgtgg gtgacctcgc tggcgccgat gtgctgggtg atgccgcccg actcgcccgc
     3421 agcgaccttc gtctcgcgca cggcgtccag caggctggtc ttgccatggt cgacgtggcc
     3481 catgaccgtc acgatcgggg cgcgggtgac gagcgtcgac gggtcgtcct cgctccagaa
     3541 gctggggcgg ccgtcggcgg actcggcgcc cggcttcacc aggtcctgct cgcgcgcctc
     3601 ctcggctgcg gcccgctcag cggccccggc ctcgaccgtc tccatgccga gctcgccggc
     3661 gaccagcgac gcggtgtcga agtcgatgga ctggttgacg gtggcgaaga tgccgttgcg
     3721 gatgaggacg ttgatgatct ccgcgttgct gacgcccagc gcctccgaga ggtccttgac
     3781 gaccacgggg ttgggcagct cgaccggccc gctcggccgc tgcggcgccg cctcggtgag
     3841 cacgccacca ggacgcggcc cgctgcgctt gcgctggggc cgtggtcctc ggcgtggtcg
     3901 gtatctattc ggcggcattc ttcacctcga cttccaacgt tcccggcgcg agctccgcgt
     3961 cgagcgctcg cctgacggcc gcggcggcgc gccccgggtc ccggcaggcc ggttcatgac
     4021 agatgtacgt cccccggccc gccagcttgc cggtcgggtc gaggtggatg agcccgtccg
     4081 ccgtgcggac cagccggtcc atctcgcgct tcgggcggga ggtccggcag accgcgcacg
     4141 tgcgggtcgg gacgtggtca ggtcgtcgct tcggcatcgt ccggcgcctc ggtgccggca
     4201 tcggcgccat gctcgccgct cccggcggcc gcggtggcca cgcccgcctc cgagcggatg
     4261 tcgatccgcc agccggtcag cttggccgcg aggcgggcgt tctggccctc cttgccgatg
     4321 gccaggctga gctgtcgctc gggaacgatg acgttcgcga tcttgtgctc ctcgtcgatc
     4381 cggacgctga gcacctccgc cgggctcagc gcctcggcga cgaagcggcc cgggtcctcg
     4441 tcccacagga cgatgtcgac cttctcgccg ttgagctcgg cgacgatggc ctggatccgt
     4501 gcgccgcgct gcccgacggc cgcgcccttg gcgtcgacgc ccggctgacg ggactcgacg
     4561 gccaccttgg atcggctgcc ggcctcgcgc gcgatccccc ggatcaccac ggtgccgttg
     4621 tagatctctg ggatctccat ctccatcagg cgacggagga agccccgatg cgtgcggctg
     4681 gccacgatga ccgggccgcg ggtcgtgcgc cgcacctcca tgacgtagac cttgaggtgc
     4741 tggccgatgc ggtacgtctc gttcggcagc tgctcggtca cggccagcac cgcctccacg
     4801 tccttgccca tgtcgagcac gacggccgaa tcggtcgtgc gctgcacgtt gccggtcatg
     4861 atctcgcctt cgcgggcggc gtactgctgg aagatcacgt cccgctgtgc ctcacgcagc
     4921 cgctggcggt agacctgccc ggccgtctgc gccgcgatcc gcccgagctg ggcctgggtc
     4981 acgttctcgg gcacgcggac cttgccgccg acgcccgtgg cgggatcgat cttctgcgcc
     5041 gcggcggccg tcatctggag ctcgggatcc tcgatctcct gctccccggc gacgacctcg
     5101 tactcgcgcg ccaccgtgat ctctcccgac tcggggttga ccgtgacgac cacgttctcg
     5161 ggcgcatcgg cattgcgccg atacgccgat tcgatcgcct gctcgagggt cctgatcagg
     5221 atctcctgcg gcacaccctt ctcggcgttc agctggagca gtgctccgat gaagtctcga
     5281 ttcatggccg ctcctcgctc gcctacccgg ttccggacga aaacaaagac gtgggatggc
     5341 gaaccgaccc acgtcctggc actaggttca atatttgtgg ttcgcattgg ccggcagaac
     5401 cga
//
LOCUS       DASTYP010000131         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_373764, whole genome shotgun sequence.
ACCESSION   DASTYP010000131 DASTYP010000000
VERSION     DASTYP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373764"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFV59_06040"
     CDS             complement(<1..836)
                     /locus_tag="VFV59_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5325427.1"
                     /translation="MTSPDAPEDAPIRSTSGIEIRPAYGPQDPPGFDPSTELGEPGGF
                     PFTRGIRPDGYRQRPWTMRQYAGFGSAQETNRRFRYLLERGQTGLSVAFDLPTQMGYD
                     SDDPLAAGEVGRVGVPIDTVADMEVLLDGIDPAAVSTSMTINATAAILLALYETVARR
                     RGADPRRLRGTIQNDILKEYIARGTYIYPPEPSMRLVTDTFAYCRAELPEWNTISISG
                     YHMREAGATAVQEIAFTIANAIAYSQAALDAGLAFDDFAPRLSFFFAAHNDLLEEVAK
                     FRA"
     gene            874..1200
                     /locus_tag="VFV59_06045"
     CDS             874..1200
                     /locus_tag="VFV59_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325428.1"
                     /translation="MPASAYGLVALFAAISAASGVLAARLIGPWRAWTAILPALAAFG
                     ALYLVGHRWVLSIGPNVKVFGWDVALPFDTAVALAVAAITALLQRTAVGLLQSQQGRS
                     GSDGLA"
     gene            complement(1153..1617)
                     /locus_tag="VFV59_06050"
     CDS             complement(1153..1617)
                     /locus_tag="VFV59_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEU5325429.1"
                     /translation="MSDYEVTVAGADTEPADGWTLHWVDRAHGVVQLRSGDRSVPLIV
                     EGEGTDWVVTIHGRRIPVTVRNWRERTLADAEREARLHGGPLTIKATLPGLVVAVAVE
                     VGSEVGEGDALLTIEAMKMQNEIRAPRAGRVIEVSVASGEAVGTGAPLLRLE"
     gene            complement(1614..>2836)
                     /locus_tag="VFV59_06055"
     CDS             complement(1614..>2836)
                     /locus_tag="VFV59_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325430.1"
                     /translation="FARAVTDAGLVFVGPPADAIEALGNKLAARRSAAAAGVPIVPGT
                     TVALTDDLASLEGLHFPVMLKAAAGGGGRGMRRVDAPDRIAEAMAAARREAESAFGDG
                     TIYVEQMVSPARHVEVQLLGDRHGNLAVLGERDCSVQRRHQKLVEESPCPGIGEATRA
                     ALFESARRVAGTVPFENAATVEFLVDAELNHYFLEMNTRLQVEHGVTELVTGLDLVAW
                     QLRVAAGEELPPSVLDAPRRGHAIEVRIYAEDPYDGFRPTHGSIGAWVMPDGPGVRVD
                     AATEADMPLRPEYDPLLAKLMVHAEDRDAAVARLRRALDETLIGGLQTDAGFLRWLVD
                     EPAFTSGGYDTSLIPARWYDRGAPLTGETMALAAFAALSVRKDSGPVARRPMASKDAG
                     SAWGRLARREALRR"
BASE COUNT          378 a         1039 c         1006 g          413 t
ORIGIN      
        1 gcgcggaact tggcgacctc ctccagcagg tcgttgtgcg cggcgaagaa gaaggagagc
       61 cgcggggcga agtcatcgaa ggcgaggccg gcgtccagcg ccgcctggct gtaggcgatg
      121 gcgttggcga tggtgaaggc gatctcctgg accgccgtcg ccccggcctc gcgcatgtgg
      181 tagccgctga tgctgatggt attccactcc ggcagctcgg cgcgacagta ggcaaacgta
      241 tcggtcacca ggcgcatgga gggctccggc gggtagatgt aggtgccccg cgcgatgtac
      301 tccttgagga tgtcgttctg gatggtcccg cgcagccgcc gcgggtcggc gccgcggcga
      361 cgggcaaccg tctcgtacag ggccagcagg atggccgcgg tggcgttgat ggtcatggag
      421 gtgctcacgg cggcggggtc gatgccgtcc aggagcacct ccatgtcggc gaccgtgtcg
      481 atgggcacac cgacccgccc gacctccccg gccgccagtg ggtcgtccga gtcgtagccc
      541 atctgcgtgg gcaggtcgaa ggccaccgac agcccggtct ggccccgttc gagcaggtac
      601 cggaagcggc ggttggtctc ctgggccgac ccgaacccag cgtactggcg catggtccag
      661 ggtcgctgcc ggtagccgtc gggccggatg ccccgggtga aggggaagcc gccgggctcc
      721 cccagctccg tcgacggatc gaagccgggt gggtcctgcg gaccgtaggc cggccggatc
      781 tcgatgcccg aggtgctgcg gatcggggcg tcctcgggcg cgtcggggga ggtcatcggt
      841 ccccgattct accgatgtgg cagactcgag ccgatgccag ccagcgccta cgggctcgtc
      901 gccctcttcg ccgccatctc cgccgcctct ggcgtcctcg ccgcccgact catcggtccg
      961 tggcgcgcct ggacggccat cctgcccgcc cttgcggcct tcggcgccct ctacctggtc
     1021 gggcaccgat gggtgctcag catcgggccg aacgtgaagg tcttcggttg ggacgtggcc
     1081 ctgccgttcg acaccgccgt ggccctggcc gtcgccgcga tcaccgcgct cctgcagcgg
     1141 acggccgtcg gcctactcca atcgcagcag gggcgctccg gttccgacgg cctcgcctga
     1201 ggcaacgctg acctcgatca cgcgaccggc ccggggcgcg cggatctcgt tctgcatctt
     1261 catcgcctcg atcgtcagca gggcgtcgcc ctcccccacc tccgacccca cctcgaccgc
     1321 gacggccacg acgaggccgg gcagggtcgc cttgatcgtc agcggcccgc catgcagacg
     1381 cgcctcgcgc tcggcatcgg ccagggttcg ctcccgccag ttgcgcaccg tgaccgggat
     1441 ccgacgtccg tggatggtga cgacccaatc ggtcccctcg ccctcgacga tcagcgggac
     1501 cgagcggtcg ccgctccgca gctgcaccac gccgtgcgcg cgatcgaccc agtggagcgt
     1561 ccacccgtcg gccggctcgg tgtcggcgcc cgcgacagtc acctcgtagt cgctcatcgt
     1621 cgcagggcct ctcgccgtgc caggcgtccc caggcggagc cggcgtcctt cgacgccatt
     1681 gggcggcgcg ccaccggacc gctgtctttg cgcacggaca gggcggcgaa cgcggccagt
     1741 gccatcgtct ccccggtcag cggcgcgccg cggtcgtacc agcgcgccgg gatcagcgag
     1801 gtgtcgtagc caccgctggt gaacgccggc tcgtcgacca gccagcgcag gaacccggca
     1861 tcggtctgga ggccgccgat gagggtctcg tccagcgccc gtcgcaggcg cgccaccgcg
     1921 gcgtcgcggt cctccgcgtg gaccatgagc ttggcgagga gcgggtcgta ctccgggcgc
     1981 aagggcatgt cggcttccgt cgcggcatcg acccgcacgc cgggaccgtc gggcatcacc
     2041 caggcgccga tgctgccatg cgtgggccgg aagccgtcgt agggatcctc ggcgtagatg
     2101 cgcacctcga tggcatggcc gcgacggggc gcgtccagga cggacggcgg cagctcctcg
     2161 ccagcggcga cgcgcagctg ccaggcgacg agatcgagac cggtgacgag ctcggtgacg
     2221 ccgtgctcca cctgcaggcg cgtgttcatc tccaggaagt agtggttcag ctccgcgtcg
     2281 accaggaact cgaccgtggc cgcgttctcg aacggaacgg tcccggccac gcggcgagcg
     2341 gactcgaaca gcgcggcgcg cgtcgcctcg ccgatccccg gacacggcga ctcctccacc
     2401 agcttctggt gtcgccgctg gaccgagcag tcccgctcgc ccagcaccgc caggttgccg
     2461 tggcgatcgc cgagcagctg cacctcgacg tggcgcgccg gcgacaccat ctgctcgacg
     2521 tagatggtgc cgtcgccgaa cgcggattcg gcctcgcgtc gcgcggccgc catcgcctcc
     2581 gcgatccggt cgggtgcgtc gacgcggcgc atcccccgtc ccccgccacc ggcggcggcc
     2641 ttcagcatca ccgggaagtg cagcccctcg agcgaggcga ggtcatcggt cagcgccacg
     2701 gtcgtgcccg ggacgatggg caccccggcg gcggccgcgg agcgtcgcgc ggccagcttg
     2761 ttgcccaggg cctcgatggc atcggccggc ggaccgacga acacgaggcc cgcatcggtc
     2821 acggcccgcg cgaagt
//
LOCUS       DASTYP010000132         9047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_9961, whole genome shotgun sequence.
ACCESSION   DASTYP010000132 DASTYP010000000
VERSION     DASTYP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9961"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFV59_06060"
     CDS             complement(<1..410)
                     /locus_tag="VFV59_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU5325431.1"
                     /translation="MTRARPAAAAAISEPAHRRTVLPSGLRVVSRSMPGSRSVSVALI
                     VPVGSRHEDEAHAGLSHLLEHLVFKGTAAHPEPGSLSQFVEGMGGMVNASTDREVTVF
                     SAKVPAARAEAALAAVSELALRPMLRRRDLAAEKP"
     gene            complement(407..910)
                     /locus_tag="VFV59_06065"
     CDS             complement(407..910)
                     /locus_tag="VFV59_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325432.1"
                     /translation="MSDARAKLYINTGVVTSKGDIYNILRPLLKQTVSYTYWRGPKVE
                     STGTGWVERIVVDHPEIASYFTPLAICLNLDSFDYLQFDTTSEQLLVYTLVVGNERVT
                     LEFAAAGVPRLGAAEDDEVRSPSGRPLPRNADELARDLRFIQMELLMAGEADPDGDRA
                     DEDEDGA"
     gene            1008..1841
                     /locus_tag="VFV59_06070"
     CDS             1008..1841
                     /locus_tag="VFV59_06070"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU5325433.1"
                     /translation="MKLLAADIGNTNTTIGLFEDGRLVRSWRATSRATATSDEVAAML
                     AGLLGLDAHRLDELDAIALASVVPPLTETWERLSIERIGVTPALVDASSLDGILDIAI
                     DRPSEAGADRLANALAARTEFGGPAIVVDLGTSTNFDLVDAAGAYIGGAIAPGMGLSL
                     EALVGRASKLPRIELHRPPSAIGASTVTAMQSGAVIGYIGLVSGLLTALRGELIERSP
                     ADARVTVVATGGYTYQPWLREVPGIDAVEPDLTLRGIGYAWAALRERDRAATRVSEPQ
                     P"
     gene            1838..3109
                     /gene="coaBC"
                     /locus_tag="VFV59_06075"
     CDS             1838..3109
                     /gene="coaBC"
                     /locus_tag="VFV59_06075"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU5325434.1"
                     /translation="MNEALGPLAGRRIVVGVSGSIAAYKAVTLVRLLAERGAVVDVAM
                     TKAATRFIGPLTFASLTHRPVLDDVFELDADQQIAHIELAEAADAIVLAPATANLLGE
                     LAAGLVGDAVTAIACASRAPVIVAPAMDAGMWTHPATQRNVATLREFGYVIVEPEVGE
                     LASGLTGIGRLAEPPTIVAQVEHLFARSGDLEGLHVVVSAGGTREPLDPVRFIGNRSS
                     GKMGAAIAEAARDRGAAVTLIAGAMSVTAPRGVTVVEATTAQTMREAVLRAAPQADIL
                     VMAAAVADFAPARASQRKIKRSGENLQIELVPNPDIVAEVGEMPDDVRPFLIGFAAES
                     TDLEANAAAKLRDKGLDLIVGNVVGGPFDAIGSDENKVTVFGAEGPLTDWPMLPKRQV
                     AERLWDLIADRYRHRREAASGDGSRGRRRRS"
     gene            3106..3960
                     /gene="panC"
                     /locus_tag="VFV59_06080"
     CDS             3106..3960
                     /gene="panC"
                     /locus_tag="VFV59_06080"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU5325435.1"
                     /translation="MIVVHHADELRDAILQRRLAGDSVGFVPTMGMLHAGQLSLVARA
                     RAENGCVVVSSFDDPGAFRSLDEPTADAHDRERDLALLAQAQADIAFIPDVMTLYPPD
                     DTTRVTVEGLTRQLEGASRPGHLDGLTTIMLKLIHLVGPARLYLGQRHAQQVVIVRRM
                     LRDLFLNVDLVICPTVRDDDGLAVSSANALLGIEDRHAATCLYQALAAAREAYDAGER
                     SAEVLRWKMTDVLSREPKARIDYVSVADARSLAELQRVSGPALALIAAWVGRVRLTDN
                     MPLAWDVA"
     gene            3977..4144
                     /locus_tag="VFV59_06085"
     CDS             3977..4144
                     /locus_tag="VFV59_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325436.1"
                     /translation="MTLTVVGYIVAAAILIAGAATAARLQSGRRATLLGAAIAVAAVV
                     AWFSWLLGTSA"
     gene            complement(4270..5379)
                     /locus_tag="VFV59_06090"
     CDS             complement(4270..5379)
                     /locus_tag="VFV59_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5325437.1"
                     /translation="MTAKRVFLAATGQNRGKTTTSLGLLAAIRDRGLRLGFVKPVGQR
                     YVVVDGGRADEDAVLMREVFDLPDALEDMSPVTLPRRFTTDYVMGRVHDDLAAQASGA
                     FDRVARDKDVVVIEGTGHAGVGAVIGLSNARVAAMLDAPVIIVSEGGVGRPIDEIVLN
                     HALFAAEGARVLGAVVNKVDVESHPHLPEVLGRGLAQHGVELLGCIAYSELLANPSLE
                     LVTTHLDGELLAGEATPGATIGWVAIGAMRADHAVELLRARTLVITPGDREDLIRAAM
                     DTNRLSASEPRVSGLVLTGGFRPDDAVLGDLRSAGLFTYLVPTDTYRTAQAVDEILVK
                     THSADTEKIATIIDLVGRAIDVDELLARLDRGPRE"
     gene            complement(5585..6190)
                     /locus_tag="VFV59_06095"
     CDS             complement(5585..6190)
                     /locus_tag="VFV59_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325438.1"
                     /translation="MLDHEERFLFREEIHNLANALADPGQLDNVEDLLAEVTTSPRFD
                     SDVFTLERSLQVMLGGRAGSTLIGLLTVDRGVFEELGEMGLPPDVHERLVSWRARFGL
                     VIDNALNFLNNPDGIQGHNFGTQLSHTESGLTLQGRLTLVRYNNEPQFFIADADVLLT
                     VAADMLERLGEHLEPEMLDPELVTRLREGVELVERRTKPRS"
     gene            complement(6270..7538)
                     /locus_tag="VFV59_06100"
     CDS             complement(6270..7538)
                     /locus_tag="VFV59_06100"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5325439.1"
                     /translation="MASQPTVAEAPNIWAVAQSQFDHAADKLDLDDGMRRVLRVPQRE
                     LTVNFPVTMDDGTVQVFTGHRVQHNVSRGPAKGGIRYHQDVSLDEVRALAMWMTWKCA
                     CVNIPYGGGKGGVIVDPKKLSIREVEGLTRRFTTEISPLIGPERDIPAPDVNTNAQTM
                     AWIMDTYSMHHGYTIPGVVTGKPIAIGGSLGRNEATARGAVFTLLQWAKATGNTLEGA
                     RVAIQGYGNAGSIAAMLLAEQGARIVAVSDSTGGIHNPAGLQPAKVSAWKQEHGTVVG
                     FPGSDEVSNRELLEVDCDILVPAALENQITKANAPRITARVVAEAANGPTTPEADAIL
                     YDRGVFLIPDILCNAGGVTVSYFEWVQDMQSFFWTEARINESLKEIMDRAFDAVHAMS
                     QRHEVDMRTAAYMVAVARVAEATMLRGLYP"
     gene            7725..>9047
                     /locus_tag="VFV59_06105"
     CDS             7725..>9047
                     /locus_tag="VFV59_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5325440.1"
                     /translation="MTDAGVLEAPPPSLSASDAERIARELFGVTASAAPLVSERDQNF
                     RLTDASGAGWVLKVANAAEDPGVVEMEVLAVERIAEVDPSLPVPRARPTLAGDPIGHG
                     EHAGQSHLVRLLPLLPGRNAAPGELSAESLGEIGEVVGRIGLALRAFFHPAAGRVILW
                     DQQRLPMLFEHASLLEPGERRDLLDRVIARVGEHVLPALPRLRSQVIHNDVTLDNLLV
                     DASGHVTGIIDFGDMAHTALVLDVPATLQSLVREREDIFEVAEPFLAGYASVLPFERR
                     EAELLGELLAGRMAQTILISAFRTRRYPDNAYIHGWSEPAWSLLGQLEAVGFEAAGRR
                     LARMARQPVAPSRSSGAGDLLQRRRRVLGSALESLSYRRPLHLVRGEGAWLEDADGRR
                     YLDAYNNVPVVGHAHPRVVEAISRQASLLNTNTRYLHEHVVELAERITS"
BASE COUNT         1246 a         3193 c         3261 g         1347 t
ORIGIN      
        1 ggcttctcgg cggccaggtc ccgccggcgc agcatcgggc gcagggccag ctcgctgacg
       61 gcggccagcg cggcctcggc cctggcggcc gggaccttgg ccgagaagac cgtcacctcg
      121 cggtcggtgc tggcgttgac catgccgccc atgccctcga cgaactgcga cagcgagccc
      181 ggctcggggt gcgcggccgt gcccttgaag acgaggtgct cgagcaggtg cgagaggccg
      241 gcatgcgcct cgtcctcgtg ccgcgagccg accggcacga tcagcgcgac gctcaccgag
      301 cgcgagccgg gcatggagcg ggagacgacg cgcagcccgc tggggaggac ggtgcggcgg
      361 tgcgctggct cggaaatggc ggcggctgcg gcggggcgtg cgcgcgtcac gcgccgtcct
      421 cgtcctcgtc ggcgcggtcg ccgtccgggt cggcctcgcc ggccatcaac agctccatct
      481 ggatgaagcg caggtcgcgg gcgagctcgt cggcgttgcg cggcagcggc cgaccactgg
      541 gcgagcgcac ctcatcgtcc tcggcggcgc ccagtcgcgg aacgccggcc gccgcgaact
      601 cgagcgtcac gcgctcattg ccgacgacga gcgtgtagac gagcaactgc tcgctggtgg
      661 tgtcgaactg caggtaatcg aaggagtcga ggttgaggca gatggcgagc ggcgtgaagt
      721 acgaggcgat ctccgggtgg tccaccacga tgcgctcgac ccaccccgtg ccggtcgact
      781 cgaccttcgg tccgcgccag taggtgtagc tgacggtctg cttcagcagc gggcgcagga
      841 tgttgtagat gtcgcccttg ctggtgacga cgccggtgtt gatgtagagc ttggcgcgcg
      901 cgtcggacat cggctcgatg gctgcggatc gtcggcgagt ctagcaccgg tccacccgtc
      961 ggtcgggccg ggcggagacg ccccgatcgg ctgctacgat acggcgcgtg aagctgctgg
     1021 cggccgacat cggcaacacg aacacgacca tcggcctgtt cgaggacggc cgcctggtgc
     1081 gctcgtggcg tgccaccagc cgggccaccg ccaccagcga cgaggtggcc gccatgctcg
     1141 ccggcctgct gggcctcgac gcgcaccggc tcgacgagct cgacgccatt gcgctggcca
     1201 gcgtcgtccc gcccctgacc gagacgtggg agcggctctc gatcgagcgc atcggcgtca
     1261 cgccggcgct ggtcgacgcg agctcgctcg acgggatcct ggacatcgcc atcgaccggc
     1321 cttcggaggc cggcgccgat cgcctcgcca acgcgctcgc cgctcggacc gagttcggcg
     1381 gcccggccat cgtggtcgac ctcggaacga gcacgaactt cgacctggtc gacgccgcgg
     1441 gcgcctacat cggcggggcc atcgctcccg gcatgggcct gagcctggag gcgctggtcg
     1501 gacgggccag caagctgccg cgcatcgagc tccaccggcc gccgtcagcc atcggcgcga
     1561 gcacggtgac ggccatgcaa tcgggtgccg tgatcggcta catcggcctc gtcagcgggc
     1621 tcctgacggc cctgcgcggc gagctaatcg agcgctcccc ggccgatgcg cgcgtgaccg
     1681 tggtggccac cggcggctat acctaccagc cgtggctgcg ggaggtcccg ggcatcgacg
     1741 ccgtcgagcc ggaccttacg ctgcgcggca tcggctacgc ctgggccgca ttgcgcgagc
     1801 gcgaccgcgc cgcgacccgc gtgagcgagc cacaaccgtg aacgaggcgc tcgggccgct
     1861 ggccggccgg cggatcgtgg tcggcgtgtc gggcagcatc gcggcctaca aggccgtcac
     1921 gctggtccgg ctgctggccg agcggggcgc agtcgtggac gtcgccatga cgaaggcggc
     1981 cacccgcttc atcggcccgc tcaccttcgc cagcctcacg caccgtccgg tcctcgacga
     2041 cgtcttcgag ctggacgccg atcagcagat cgcgcacatc gagctggcgg aggcggccga
     2101 tgccatcgtc ctcgccccgg cgacggccaa cctgctcggc gagctggccg ccgggctggt
     2161 cggcgacgcc gtgacggcca tcgcctgcgc ctcgcgagcg cccgtgatcg tcgctccggc
     2221 catggacgcc ggcatgtgga ctcaccccgc cacccagcgc aacgtcgcca cgctgcggga
     2281 gttcggctac gtcatcgtgg agcccgaggt cggcgagctc gcctcggggc tcaccggcat
     2341 cggtcgcctg gccgagccgc cgaccatcgt cgcgcaggtc gagcacctct tcgcccgctc
     2401 cggcgacctc gaagggctgc acgtggtggt cagcgccggc gggacgcgag agcccctcga
     2461 cccggtgcgg ttcatcggca accgcagcag cggcaagatg ggcgccgcca tcgccgaggc
     2521 ggcacgcgac cgaggagccg ccgtgacgct gatcgccgga gccatgagcg tgaccgcccc
     2581 gcgtggcgtc accgtggtcg aggccaccac ggcccagacc atgcgcgagg ccgtgctccg
     2641 cgccgcgccc caggccgaca tcctggtcat ggccgccgcc gtcgcggact tcgcaccggc
     2701 ccgcgcgagc cagcgcaaga tcaagcgctc cggcgagaac ctgcagatcg agctcgtgcc
     2761 gaacccggac atcgtggccg aggtgggcga gatgccggac gacgtccggc cgttcctcat
     2821 cggcttcgcg gcggagtcca ccgacctgga ggccaacgcg gccgccaagc tgcgcgacaa
     2881 gggcctcgac ctcatcgtcg gcaacgtggt gggcggcccg ttcgacgcca tcggctcgga
     2941 cgagaacaag gtcaccgtct tcggtgccga aggcccgctc accgactggc cgatgctccc
     3001 caagcgccag gtcgccgagc gcctctggga cctgatcgcg gaccgatacc ggcaccgccg
     3061 cgaggcggcc agcggggacg gcagccgcgg ccgacgccgc cgctcgtgat cgtcgtccac
     3121 cacgccgacg agctgcgcga cgcgatcctg cagcggcggc tcgccggcga cagcgtcggc
     3181 ttcgtcccga ccatgggcat gctccacgcc ggacagctct ccctggtggc gcgcgcccgc
     3241 gccgagaacg gctgcgtcgt cgtctcctcc ttcgacgacc ccggcgcgtt tcgttcgctc
     3301 gacgagccga cggccgatgc gcacgaccgg gagcgcgacc tcgcgctgct ggcgcaggcg
     3361 caggccgaca tcgccttcat ccccgacgtg atgaccctct acccgcccga cgacacgacg
     3421 cgggtcaccg tcgaggggct gacgcgccag ctcgagggtg cgtcgcggcc ggggcacctg
     3481 gacggcctga ccaccatcat gctcaagctg atccacctgg tcggcccggc acgcctctac
     3541 ctgggccagc gccacgccca gcaggtggtc atcgtgcgac gcatgctgcg cgacctgttc
     3601 ctcaacgtgg acctcgtcat ctgcccgacg gtccgcgacg acgacggcct ggccgtctcg
     3661 agcgcgaacg cgctgctggg catcgaggac cgccacgccg caacgtgtct ctaccaggcg
     3721 ctggccgccg ccagggaggc gtacgacgcc ggcgagcggt cggccgaggt gctgcgctgg
     3781 aagatgaccg acgtgctctc gcgagagccg aaggcgcgca tcgactacgt cagcgtcgcg
     3841 gatgcgcgct ccctggccga gctgcagcgc gtcagcggcc cggcgctggc cctgatcgcg
     3901 gcgtgggtgg gtcgcgtccg gctgacggac aacatgccgc tggcgtggga cgtcgcctga
     3961 gcccggtgag gggcgcatga cgctgaccgt ggtcgggtac atcgtcgccg cggcgatcct
     4021 catcgcgggc gccgccactg cggcccgcct gcagtccggc cgacgcgcga ccttgctcgg
     4081 ggcagcaata gccgtggctg ccgtcgtggc gtggttctcg tggctcctcg ggacctcggc
     4141 ctgaaagtac tagtgcggtc cccggggcga atcctatgat gcgaagcgtc accagacgcc
     4201 ggcccgcacg ggccgtgttg cgtcaggtgc atgggaggcc ggcgggtgcc cacgtccggc
     4261 ctcccttcgt cattcccgcg ggccgcgatc cagtcgggcg agcagctcgt ccacgtcgat
     4321 cgcccgcccc accaggtcga tgatggtggc gatcttctcc gtgtccgcgg agtgcgtctt
     4381 gaccaggatc tcgtcgacgg cctgtgcggt gcgataggtg tccgtcggca cgaggtaggt
     4441 gaagaggccg gccgagcgca ggtcgccgag gaccgcgtca tccggacgga agcccccggt
     4501 caggaccagg ccgctcaccc ggggttcgga cgccgagagc cggttcgtgt ccatggcggc
     4561 gcgaatgagg tcctcgcggt ccccgggcgt gatcaccagg gtgcgggccc gcagcagctc
     4621 cacggcgtga tcggcccgca tcgccccgat ggcgacccag ccgatggtgg cgcccggggt
     4681 ggcctcgcca gccagcagct ccccgtcgag gtgcgtggtc acgagctcga gcgacgggtt
     4741 cgccagcagc tccgaatacg cgatgcagcc cagcagctcc accccgtgct gcgccaggcc
     4801 acggcccagc acctcgggca ggtgcgggtg cgattcgacg tccaccttgt tcaccaccgc
     4861 gcccaggaca cgggcgccct cggcggcgaa cagcgcgtgg ttgagcacga tctcgtcgat
     4921 cggccgaccg acgccgccct cgctgacgat gatcaccggc gcatccagca tggcggccac
     4981 ccgcgcgttg gagaggccga tgaccgcgcc cacgcccgcg tgccccgtcc cctcgatcac
     5041 caccacgtcc ttgtcgcgtg cgacgcggtc gaaggcgccg ctcgcctgcg cggcgaggtc
     5101 gtcgtggacg cgccccatga cgtagtcggt ggtgaagcgc cgtggcaggg taaccggcga
     5161 catgtcctcg agcgcatcgg gcaggtcgaa gacctcgcgc atcaggaccg cgtcctcgtc
     5221 ggcacggcca ccgtcgacga ccacgtagcg ctggccgacc ggcttcacga accccaggcg
     5281 cagccctcgg tcccggatgg cggccagcag tccgaggctg gtggtcgtct tgccgcggtt
     5341 ctggccggtc gccgccagga agacccgttt cgccgtcacg tttggcactt tatctggcgt
     5401 ctcggtagat cgaggcgaat tcacgcgacg acgccgcgtc gctgcattcc cgcgatctgt
     5461 cgagccctgc gactcacgag cgctacgtca ctacgcgctt cagaccgttg cctctcgatg
     5521 agtcgcgaaa cccgcgcccg agccagtcgc cgggcgaggc tggctcgatc catcgagcca
     5581 gccgtcagga gcgcggcttg gtccgacgct ccacgagctc gacgccctca cgcagccgcg
     5641 tgacgagctc ggggtcgagc atctccggct ccagatgctc gcccagccgc tcgagcatgt
     5701 cggcggcgac ggtgagcagc acgtccgcat cggcgatgaa gaactggggc tcgttgttgt
     5761 agcgcaccag ggtcagccgg ccctggagcg tcaggccgga ctcggtatgc gacagctgcg
     5821 tcccgaagtt gtggccctgg atgccgtcgg ggttgttcag gaagttcagg gcgttgtcga
     5881 tgacgagccc gaatcgggcg cgccacgaga ccagccgctc gtggacgtcg ggtggcagcc
     5941 ccatctcgcc gagctcctcg aagacgccac ggtcgacggt cagcaggccg atcagcgttg
     6001 agccggcgcg gccgccgagc atgacctgga gcgagcgctc gagggtgaag acgtccgaat
     6061 cgaagcgggg gctggtcgtc acctcggcca gcaggtcctc gacgttgtcg agctggcccg
     6121 gatcggccag ggcgttggcg aggttgtgga tctcttcccg gaagagaaag cgctcttcgt
     6181 ggtccagcat cggtgtccct ccggtgggcg gaccgatgcg atcgaggggc gccgcgcacg
     6241 tggcgcggcg cccgcatcgg tacggtggct tacgggtaga ggccgcgcag catggtcgcc
     6301 tcggccacgc gggccaccgc caccatgtac gccgcggtcc gcatgtcgac ctcgtggcgc
     6361 tggctcatgg catgcaccgc gtcgaaggcg cgatccatga tctccttgag gctctcgttg
     6421 atccgtgcct cggtccagaa gaagctctgc atgtcctgga cccactcgaa gtagctgacg
     6481 gtcacaccgc ccgcgttgca caggatgtcc gggatcagga agacgccgcg gtcgtagagg
     6541 atggcgtcgg cctcgggcgt ggtggggccg ttggccgcct cggcgacgac cctggcggtg
     6601 atgcgcgggg cgttggcctt ggtgatctgg ttctccagcg ccgccggcac caggatgtcg
     6661 cagtcgacct cgaggagctc tcggttcgag acctcgtccg agccggggaa gccgacgacg
     6721 gtgccgtgct cctgcttcca ggcgctcacc ttggccggct ggagtcccgc cgggttgtgg
     6781 atgccgccgg tcgagtcact gaccgcgacg atgcgggcgc cctgttccgc gagcagcatg
     6841 gcggcgatgg agccggcgtt gccgtagccc tggatcgcca cccgtgcgcc ctcgagcgtg
     6901 ttgccggtgg ccttggccca ctgcagcagg gtgaagaccg cgccgcgcgc cgtcgcctcg
     6961 ttgcgcccga gcgagccgcc gatggcgatg ggcttgcccg tgaccacgcc ggggatcgtg
     7021 tagccgtgat gcatggagta ggtgtccatg atccaggcca tggtctgggc gttggtgttg
     7081 acgtcgggcg ccggaatgtc tcgctcgggc ccgatgagcg ggctgatctc ggtcgtgaag
     7141 cggcgggtca ggccctccac ctcgcggatc gacagcttct tgggatcgac gatgaccccg
     7201 cccttgccgc cgccgtacgg aatgttgacg caggcgcact tccaggtcat ccacatggcc
     7261 agggcgcgaa cctcgtccag gctgacgtcc tggtggtagc ggatgccgcc cttggctggt
     7321 ccgcggctga cgttgtgctg gacgcggtgt ccggtgaaga cctggaccgt gccgtcgtcc
     7381 atggtcacgg ggaagttgac ggtgagctca cgctgcggca cacgcaggac acgtcgcatc
     7441 ccgtcatcga ggtcgagctt gtcggcggcg tggtcgaact ggctctgggc gaccgcccag
     7501 atgttcggag cctcggccac cgtaggttgg ctggccatcg gatgtgctga ctgcctcctg
     7561 tgcgagacgg acgccggcgc ctcttcgcgc cactggtcga aggcggaggc aggccgacgg
     7621 tcgctgtgac ggttctcgat gttactgcac gacggctgcc ggaacggggc ccgaatggcc
     7681 ccgccgggat ggtgcgttcg gcccgggccg ctaggatcac cggcgtgacc gatgccggcg
     7741 tcctcgaggc gccacctccg agtctctcgg cgtccgacgc cgagcgcatc gcgcgcgagc
     7801 tgttcggcgt gacggcgtcg gccgcgcccc tggtgagcga gcgcgaccag aacttccgcc
     7861 tgaccgatgc ctccggcgcc gggtgggtcc tgaaggttgc gaacgccgcg gaggaccccg
     7921 gcgtcgtcga gatggaggtg ctggcggtcg agcgcatcgc cgaggtcgat ccgagcctgc
     7981 cggtgccacg ggcccggccg accctcgccg gcgacccgat cggacacggc gagcacgcgg
     8041 gccagtccca cctcgttcgg ctgctgccgc tgttgccggg ccgcaatgcc gccccgggtg
     8101 agctgagcgc ggagagcctc ggcgagatcg gcgaggtcgt cgggcgcatc ggcctggcgc
     8161 tgcgcgcgtt cttccatccc gcggctggcc gcgtgatcct gtgggaccag cagcgtctgc
     8221 cgatgctgtt cgagcacgcg tcgctgctgg agccgggcga gcgacgtgac ctgctggatc
     8281 gggtgatcgc ccgtgtggga gagcacgtgc tgccggccct gccgcgcctg cggtcgcagg
     8341 tcatccacaa cgacgtgacc ctcgacaacc tgctggtcga tgcgtcaggg cacgtcaccg
     8401 ggatcatcga cttcggggac atggcgcaca cggccctggt gctggacgtg ccggccacgc
     8461 tgcagtcgct cgtccgcgag cgcgaggaca tcttcgaggt ggccgagccg ttcctggcgg
     8521 gttacgcatc ggtcctgccg ttcgagcgcc gggaggcgga gctgctgggc gagctgctgg
     8581 cgggacggat ggcgcagacg atcctgatca gcgccttccg gacgcgccgc tacccggaca
     8641 acgcctacat ccacgggtgg tcggagccgg cctggtcgct gctgggacag ctcgaggcgg
     8701 tcgggttcga ggcggcggga cgccgcctgg cccggatggc acggcagccg gtggcgccgt
     8761 cgcgttcctc gggcgcgggg gacctgctgc aacggcgccg tcgggtgctc gggagcgccc
     8821 tggagtcgct gagctatcgc cgtccgctgc acctggtccg gggcgagggc gcctggctcg
     8881 aggacgcgga cgggcgccgc tacctagacg cgtacaacaa cgtgcccgtc gtcggccacg
     8941 cccatccgcg ggtggtcgag gcgatctcgc ggcaggcatc gctgctcaac accaacacgc
     9001 ggtacctgca cgagcacgtc gtcgagctgg cggagcggat cacatcg
//
LOCUS       DASTYP010000133        12500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1612896, whole genome shotgun sequence.
ACCESSION   DASTYP010000133 DASTYP010000000
VERSION     DASTYP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612896"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..326
                     /locus_tag="VFV59_06110"
     CDS             78..326
                     /locus_tag="VFV59_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325441.1"
                     /translation="MTQSRDDLRNDEVDRIPLLQPGAHLEQGSVYLDLDDIDAGPFVA
                     VGHERVHEDDRLVAKRDADDESWDRLAGKRQPRVLRPI"
     gene            complement(334..519)
                     /locus_tag="VFV59_06115"
     CDS             complement(334..519)
                     /locus_tag="VFV59_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325442.1"
                     /translation="MTDRSRPRRPTEDERRFVDTLLFLRGLYAARGPRDAEPVASIPT
                     VMAAAQAALVAGTVGAA"
     gene            complement(631..1410)
                     /locus_tag="VFV59_06120"
     CDS             complement(631..1410)
                     /locus_tag="VFV59_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325443.1"
                     /translation="MHELSAILAIAHRDLVKLLRDRARLVADLTFPMIFIGVLGTSLQ
                     AGFGEGSGFDLLTFVFTGVLAQTLWQSAALGVISLIEDRENDFSQEIFVSPISRYSIV
                     IGKVLGESLVALPQGIAIVAFAAIIGIPLSLGVILALIPAVLVTTLFGGAFGVLVLSR
                     LPNRRAATQIFPFVMLPQFFLAGVFNPISSLPPILDVLSRISPMRYAVDLVRNAYYAV
                     HPEPTDIALASAGTNLAIIGVLFLVFIAAGTAMFVRAEQNR"
     gene            complement(1403..2353)
                     /locus_tag="VFV59_06125"
     CDS             complement(1403..2353)
                     /locus_tag="VFV59_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5325444.1"
                     /translation="MIHVDHLVKRYRKAERNAVDGITFDVRAGEFFALLGPNGAGKTT
                     TISILTTTLSPTAGTATIDGNDIVREASAVRRKVGIIFQRPSLDRNLTGEENVRFHAV
                     LYGVYPYAPSYRLMPSEYREHVAQLASIVGLGDEVFAPIRTYSGGMSRKLEIVRSLIH
                     RPKVLFLDEPTSGLDAPSRRALWAYLEQVRREANTTILLTTHYLEEAEEADRICIIDH
                     GRVISLGTPAEIKADLVEEYVLVDAAPSQRSSLAAELAGLGLSASGDGPFRIELNGRT
                     THAVLKAIDTPLTVVRTHAPSLEDAYLEIIGRSEEDGTDA"
     gene            complement(2953..3690)
                     /locus_tag="VFV59_06130"
     CDS             complement(2953..3690)
                     /locus_tag="VFV59_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325445.1"
                     /translation="MTDGEPEAGLTLRRLIASIVTAPSTDLPPGGLGAELTGWATGAP
                     AFRAYLERYRDKIRKKLRGSADDEARADVRAELLFARLLLANRRFELVFEAHGSQRRG
                     PDYSVLFRRTTPFDVEVTRLRRAPTEGAVVGTTAAKVRQLTAGTPNVLVLACDAVDAR
                     ALDLSAAMATLRRRADAWDGTFFGTRGFDTRRAFYDRFLRLGALVAWCPDTDGQARAD
                     AWVNPAARTAAPHRALAAVVEALRARP"
     gene            3778..6345
                     /locus_tag="VFV59_06135"
     CDS             3778..6345
                     /locus_tag="VFV59_06135"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5325446.1"
                     /translation="MTQADEDLRPVPGVAIRPGDNAATRALLDATDWSATSLGLREAW
                     PASLRSYVDLVMGLPSPAIVFWGDDQAQIYNDGYAVIMGPRHPRYFGAPYRESWPDTY
                     PVIYPWMRRVLDAGEIVTVDRSEIMVTRYGFPEEAYFTFTFSPLRDDEGRITGIFQPV
                     VEVTAQVLADRRAESLRALADQIDAIDPFGPPIGALATEPRDIPFALVYRWDADADAL
                     RLAGHTESLTDDAAALDAVAGRARAVYEGGQAMELSADEIESLVHADAGPWPEPTRRA
                     IAMPMPGFPDRPTGVAVFGISPRLHFDDAYRAFLGQVAAQLGAAFARGELRRSEQDAR
                     AELDVAHRSIRHRTAQFEQLLGMAPVGVYLVDAHFRIREVNPVARPFFEGIRDLIGRD
                     FRDVVRRLHSPERAEALIEIFRDVLETGESHAETEQPQQRPGTDRTAYFEWRVDRIPL
                     PDGGNGAVCYFRDVSEQILARRALEDANRRKDEFLAMLGHELRNPMAPIRASAQLLRM
                     SGTTPETRERAVDIIERQTRHLTRLVDDLLDVSRIMQGKVTLVPRRVELAAIVDQAAE
                     TVRPFIDARGHTLTIDVPRGGLQLEADETRMVQVVGNLLNNASKFTPERGSISVSARP
                     DGDQVCLEVRDDGIGMPAELIPDVFELFVQGRPSLDRSHGGLGIGLTLVRQLVRLHGG
                     EVTAESDGVDRGSVFTVRLAAVSAPVPLDGDGASARGGADRLRVLVVDDNVDMAETTA
                     ALLEMDGHEIDITFDGPSAVERVEAFQPSVVLCDIGLPGMDGYELATHLRARYPDLRL
                     IALTGYGHDDARRDGERAGFDHYLVKPVDFQLLSRLLHPRSADRSDGSWRADGTDGA"
     gene            complement(6359..6712)
                     /locus_tag="VFV59_06140"
     CDS             complement(6359..6712)
                     /locus_tag="VFV59_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009882788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5325447.1"
                     /translation="MISIGSVVFRVSDLERMTAFWSAALDYVPRPENDDDFVLMRPRS
                     GTGPNISLDRHHSSYGLPPRIHLDLYAEDQRAEVERLRSLGAREVHWDRRPADADYVI
                     MEDPEGNRFCVVDAG"
     gene            complement(6709..7116)
                     /locus_tag="VFV59_06145"
     CDS             complement(6709..7116)
                     /locus_tag="VFV59_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5325448.1"
                     /translation="MNDESREADELTAARIGAITLFVDDLGATKQFYLDVFGKPLVFE
                     DAESAVFRFGDTLINLLRTTAAHELIAPATVGAAGTDARAQLTVEVSDVDATCEELRR
                     RGVTLLNGPMDRPWGIRTAAFRDPAGHIWEIAR"
     gene            complement(7128..7856)
                     /locus_tag="VFV59_06150"
     CDS             complement(7128..7856)
                     /locus_tag="VFV59_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5325449.1"
                     /translation="MNQRTHAAPIILVPGLWLGAWAWDEVVAILRADGHDVTALTLPG
                     FEAPETDRSSITLSDHVETICDAVRAADAPVVLAVHSAAGFSGYAASDRVPERIAAMV
                     YVDTAPGKGALEPDFEGAERPMNWDEVSAEENLDGLSEEQLATFRERALPVPGGVLSE
                     QIELTNDARRDIPSTFICTGFTAEQYQQTAREHPEWPFLAGLPEIRNATWIDLPTSHW
                     PMWSRPRELAKIMGRVARAHAPQQ"
     gene            complement(7853..8491)
                     /locus_tag="VFV59_06155"
     CDS             complement(7853..8491)
                     /locus_tag="VFV59_06155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325450.1"
                     /translation="MVPANEAPWDDVAAVFGTRGASAACFCQRYKLHPREAFSRFPAS
                     ERAERLREQTHAGQPTATTTTGLIAYLGDQPVGWCAVQPRADLFGLRRVYRTPWEGRG
                     EDRDDPTVWSVTCVLVRPGYRGRGIAYALAAAAVQHARVRGARALEAYPMRREVGEVT
                     WDEIHVGAESIFAAAGMSEVSRPGKRRVVMRVDFHPSGSATIEDPRKHTGAP"
     gene            complement(8509..9417)
                     /locus_tag="VFV59_06160"
     CDS             complement(8509..9417)
                     /locus_tag="VFV59_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325451.1"
                     /translation="MHVTNWLLDADPAIRWQVLRDLTDAPATEVAAERARVAREGWGA
                     ALLAAQDADGRWGGGTFFPRGTGTFDTLHLLHQLGIEPQSPELQRALEPVHEAARWDY
                     DPNFRFFDGEVEPCINGRVVGMGAAWGRDVRGIVDRLLNEQMDDGGWNCEQENGSTRG
                     SFDTTINVLEGLREFERASGGDGAVAAARERGEDYLLARHLLHRLSDGEVGQDRWLEV
                     GFPYSWEFDVVRVLDYLRASGAAADERMSEALDVVESKRGADGRWPLETRYHEQLHVD
                     LGEHVGAASRWITLKALRILRWAGRS"
     gene            9517..10197
                     /locus_tag="VFV59_06165"
     CDS             9517..10197
                     /locus_tag="VFV59_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5325452.1"
                     /translation="MSDSTIRPITPRAFEDAHGMDDWRVLGDGAACFFRTASFPESAR
                     FVAALAADATIAGHPPAVDVRHDGVTLRLVTASEDHFGMTELDVEVARAIGAIARRLG
                     LTADASVLQSILVVPGAPDTAAVFPFWREVLGYQPRPDSPEEDLVDPGDRGPGFWFEA
                     MDEPRGDGGGAIHIGVFVPHEVAESRVHAALAAGGRMVRDAYAPHWWTLADAAGNEAD
                     IGVIRDRE"
     gene            complement(10209..10775)
                     /locus_tag="VFV59_06170"
     CDS             complement(10209..10775)
                     /locus_tag="VFV59_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5325453.1"
                     /translation="MTTGRVRYAMNVSLDGFVADAAGGLDWGLIDDELHQWFNDRART
                     LAGSLYGRRLYEVMTAYWPTGEDDPQSTDVMREFARIWNATPRFVFSTTLEGVEHNSR
                     LVRGDVREVLAEVRRELDGDLEVGGPDLASQFLRHGLVDDVELVVHPVVLGGGLRFWP
                     SLEAPLAYRLQEVKRFASGVVALSYARR"
     gene            complement(10772..11656)
                     /locus_tag="VFV59_06175"
     CDS             complement(10772..11656)
                     /locus_tag="VFV59_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5325454.1"
                     /translation="MTLTHTEPTTRTLDVPGATLTYDVRLNPGSSEPPLFLIGSPMAA
                     AGFTTLASHFPDRTVVTYDPRGSERSTKLDATGPSTPEEHADDLHRLIQAVGLGPVDL
                     FATSGGAVNAMALVRAHPHDVRTLVAHEPPIIDVLPDREAAKAASRQVHADYQRYGFG
                     VGMARFITLVMHEGPLPDDWASQPAPDPAMFGLPTEDDGSRDDPLLGQNMESSPEYVP
                     DFDALRSGPTRIVMAAGEESLRTLTGRSTVAIAERLGLEPTVFPGNHAGFLGGEYGQT
                     GKPDEFASTLRAVLEGEG"
     gene            complement(11740..12249)
                     /locus_tag="VFV59_06180"
     CDS             complement(11740..12249)
                     /locus_tag="VFV59_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5325455.1"
                     /translation="MPVTAVTKDPQALTMTVEAEFDATPERVWQLWADPRQLERWWGP
                     PTYPATFTRHELTPGSRVEYHMTGPEGDQPRGYWDVLETDPPRSLVVRDGFANDDGSP
                     NETMPAGEMRVAIEEIGGGRTRMSIYNIFPSTEAMEQLLAMGQEEGMKQALGQIDAIL
                     AEDPAAARA"
     gene            complement(12249..>12500)
                     /locus_tag="VFV59_06185"
     CDS             complement(12249..>12500)
                     /locus_tag="VFV59_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5325456.1"
                     /translation="EGVAELASHYPMSFAAVQKHVAILERAGLVTKQRIGRRKVVRTN
                     LAGLIAARRLLDQYEELWRGRIDRMAALIADSPTPQEAD"
BASE COUNT         1784 a         4410 c         4460 g         1846 t
ORIGIN      
        1 ggctgatgga ggacggaggc gcctgaggtc atgcgccaag tatcggcgac cggcacccat
       61 cctgcggtcg cgtcggtatg acccagtcac gagacgacct gcgcaacgac gaggtcgatc
      121 gcatcccgct cctccagccc ggggcgcacc tggagcaggg gagcgtgtac ctggacctcg
      181 acgacatcga cgccggaccg ttcgtcgccg tgggacatga acgcgtgcac gaggacgacc
      241 ggctcgtcgc gaagcgcgat gccgacgacg agagctggga ccgcctggct gggaagcgtc
      301 agccccgggt cctgcgcccc atctgacgac gcgtcaggct gcgccgacgg tccccgccac
      361 gagcgccgcc tgcgccgcgg ccatcaccgt cgggatgctc gcgaccggct cggcgtcacg
      421 tggcccgcgg gccgcgtaca ggccgcgcag gaagaggagc gtgtccacga atcggcgctc
      481 gtcctcggtg ggtcgtcggg ggcggctgcg gtcagtcatg cccggatggt cggtgacgga
      541 gccgccccgc gacgagcgcc gggacgacgc atcgcctgtc gtcttcgcgt cagtcgacgc
      601 gtcgcatcgg cgtcgtgagc ctcccgtccg ctaacggttc tgctcggccc gcacgaacat
      661 cgcggtcccg gccgcgatga agaccaggaa cagcaccccg atgatcgcca ggttcgttcc
      721 cgccgatgcg agcgcgatgt cggtcggctc gggatggacg gcgtagtacg cgttgcggac
      781 gaggtccacc gcgtagcgca tcggcgagat gcgcgacagc acgtccagga tcgggggcag
      841 gctgctgatc gggttgaaga cacccgccag gaagaactgc ggcagcatca cgaacgggaa
      901 gatctgggtc gcggcccgac ggttgggcag gcgcgacagc acgaggacac cgaaggcgcc
      961 accgaacagg gtggtgacga gcaccgccgg gatcagcgcc aggattacgc ccagcgagag
     1021 ggggatcccg atgatggctg cgaacgcgac gatggcgatg ccctgcggca gggccacgag
     1081 cgactcgccg agcaccttgc cgatgacgat cgagtagcgc gagatggggg agacgaagat
     1141 ctcctggctg aagtcgttct cgcggtcctc gatgaggctg atgacgccga gcgcggccga
     1201 ctgccacagc gtctgggcca ggacccccgt gaagacgaac gtcagcaggt cgaagccgct
     1261 gccctctccg aacccggcct gaaggctggt ccccagcaca ccgatgaaga tcatcgggaa
     1321 ggtcaggtcc gcgacgagcc gggcccgatc ccggagcagc ttgacgaggt cccgatgcgc
     1381 gatggccagg atggccgaca gctcatgcat ccgtgccgtc ctcctccgag cggccgatga
     1441 tctcgaggta ggcatcctcc aggctgggtg cgtgcgtccg cacgacggtc agcggcgtat
     1501 cgatggcctt gagcaccgca tgcgtggtcc gcccgttcag ctcgatgcgg aagggaccgt
     1561 ctccgctggc cgacaggccg aggccggcca gttcggccgc cagggagctc cgctgtgacg
     1621 gcgcggcgtc gaccagcacg tactcctcca ccaggtccgc cttgatctcg gccggcgtcc
     1681 cgagcgagat gacgcgcccg tggtcgatga tgcagatgcg gtcggcctcc tccgcctcct
     1741 cgaggtagtg cgtggtcagc aggatcgtgg tgttcgcctc gcggcggacc tgctccaggt
     1801 acgcccacag cgcccgacgg ctcggcgcgt cgagcccgga ggtcggctcg tccaggaaca
     1861 gcaccttcgg gcgatggatg aggctccgca cgatctcgag cttgcggctc atcccgccgg
     1921 agtaggtgcg aatcggggcg aagacctcgt cgccgaggcc gacgatcgag gccagctgcg
     1981 cgacgtgctc gcggtactcg ctcggcatca ggcgatacga cggggcgtac gggtagacgc
     2041 cgtacaggac ggcgtggaac cgcacgttct cctcgccggt caggttccgg tccaggctcg
     2101 gacgctggaa gatgatcccg accttgcgcc gcacggcgct cgcctcccgg acgatgtcgt
     2161 tgccgtcgat cgtggcggtc ccggcggtcg gggagagggt cgtggtcagg atcgagatgg
     2221 tggtcgtctt gccggcaccg ttcggtccga gcagggcgaa gaactcaccg gctcgaacgt
     2281 cgaacgtgat cccgtcgacg gcgttgcgct ccgccttgcg gtagcgcttc acgaggtggt
     2341 cgacgtggat catcagccgc cgattgtcgc aggtcgctga accgtcatcg actgcttgga
     2401 ccgatccgag cggcgcatct catcggtcgc caggccttgt ggcacggcgc gtgcgttaga
     2461 gcgcgcccgc atcggctctg ccttctccag gggaacgacc ttgatccatc ggctctctcg
     2521 cgtgctcggc ctcgtgacac gggtcatcgg cgtgcgcgag ctgagcgcca tccctggcct
     2581 gctcctcgtc catgcgccgg ccctctggct ggcgtccagc gcggtcgaga ccgtcaggga
     2641 catcgcggtc ctcctgcgcg cgcagtccga tgcgacccgc cgcgcgcgcc tgatggtggc
     2701 caccctcgcc gtcgtcggtg ccctcggcgt cgacctgggt accaccacgg cggcgcggcg
     2761 cgagcagccg caggctcccg gtccgatcgc ggtcgcggcc gttgcatcgg tgctggcgac
     2821 cgggcctcgg gcgggtcgcg gccggcgagc tcgttcgttc ctgcgtcgtc ctcgcccggc
     2881 gccgaccacg ggatgtggcg gccgagcgcg gcgatcgcgc gcgtcaggag ctcgacggct
     2941 ggggttggtg cgtcacggcc gggctcgcag cgcctcgacg acggccgcca gcgctcgatg
     3001 gggagcggcc gtgcgcgcgg ccggattcac ccacgcgtcg gctcgtgcct gcccatcggt
     3061 gtcggggcac caggcgacga gcgcgccgag gcgcaggaag cggtcgtaga aggcgcggcg
     3121 cgtgtcgaac ccgcgcgtgc cgaagaaggt cccgtcccac gcatcggcac gacgacggag
     3181 ggtcgccatg gccgcgctga gatcaagggc gcgggcgtcg accgcgtcgc aggcgaggac
     3241 gagcacgttc ggtgtgccgg cggtcagctg gcggaccttg gccgcggtcg tcccgacgac
     3301 ggccccctcg gtcggcgcgc gacgcagccg cgtgacctcg acgtcgaagg gcgtcgtccg
     3361 gcggaacagg accgagtagt cgggaccgcg acgctgggac ccgtgcgcct cgaacaccag
     3421 ctcgaaccgt cgattcgcca gcaggagccg ggcgaacagg agctcggccc ggacgtcggc
     3481 ccgcgcctcg tcatcggccg agccgcggag cttcttgcgg atcttgtcgc ggtagcgttc
     3541 caggtaggcc cggaacgccg gagcgccggt ggcccagccg gtgagctcgg cacccaggcc
     3601 gccgggcggc aggtccgtgg acggcgcagt gacgatcgag gcgatcaggc gccgcagcgt
     3661 cagtccggcc tccggttcgc catcggtcac gccatcagcg tacgcgtctg gttcttcgta
     3721 cggctcgcgg gcatgccagt tgcttaggcc ctcaggaact ccgcacgacc cgagcccatg
     3781 acgcaagccg acgaggacct gagaccggtc cccggcgtcg ccatccgtcc cggcgacaac
     3841 gcagcgacac gcgccctcct cgacgcgacc gattggtcgg cgacgagcct ggggctgcgc
     3901 gaagcatggc ccgccagcct gcgcagctac gtcgacctgg tcatggggct cccgtccccg
     3961 gcgatcgtct tctgggggga tgaccaggcg cagatctaca acgacggcta cgccgtgatc
     4021 atgggcccgc ggcacccgcg ctacttcggt gccccgtacc gcgagtcgtg gccggacacc
     4081 tacccggtga tctacccgtg gatgcgccgc gtcctcgacg ccggcgagat cgtgaccgtc
     4141 gaccggtccg agatcatggt cacgcgctac ggcttccccg aggaggcgta cttcaccttc
     4201 accttcagcc cgctgcgcga cgacgagggc aggatcaccg gcatcttcca gcccgtcgtc
     4261 gaggtgacgg cccaggtcct cgcggatcga cgggcggagt cgctgcgcgc gctcgccgac
     4321 cagatcgacg cgatcgatcc cttcggtcct cccatcgggg cgctcgcgac cgagccgcgt
     4381 gacatcccct tcgcgctcgt ctaccgctgg gacgccgatg ccgacgccct tcgcctcgcg
     4441 ggccacaccg agagcctgac cgatgacgcc gctgccctcg atgccgtcgc cggacgggct
     4501 cgtgccgtct acgagggcgg tcaggccatg gagctgtccg ccgacgagat cgagtccctc
     4561 gttcatgccg acgcggggcc atggccggaa ccgacgcgcc gggccatcgc catgccgatg
     4621 cccggcttcc cggaccggcc cacgggtgtc gcggtgttcg gtatcagccc acggctgcac
     4681 ttcgacgatg cgtaccgcgc gttcctgggc caggtggcgg cacagctcgg tgccgcgttc
     4741 gcgcgtggcg agctgcgtcg gagcgagcag gacgcccgcg cggagctgga cgtcgcgcac
     4801 cgctcgatcc gccaccgcac cgcgcagttc gagcagctgc tcggcatggc ccccgtcggc
     4861 gtgtacctcg tcgacgcgca cttccggatt cgcgaagtga acccggtggc gcgcccgttc
     4921 ttcgagggga ttcgagacct catcggccgt gatttccggg acgtggtgcg gcggctgcac
     4981 tcgccggaac gtgcagaggc gctgatcgag atcttccggg acgtcctcga gaccggggag
     5041 tcacacgcgg agacggagca gccccagcag cggcccggca ccgatcgaac cgcctacttc
     5101 gagtggcgcg tcgacaggat tccgctgccg gacggcggca acggggcggt gtgctacttc
     5161 cgcgacgtct cggagcagat cctggcgcga cgcgcgctcg aggacgccaa ccgtcgcaag
     5221 gacgagttcc tggcgatgct cgggcacgag ctgcggaacc cgatggcgcc gatccgggcc
     5281 agcgcgcagc tgctgcgcat gagcggcacc acccccgaga cgcgcgagcg cgccgtcgac
     5341 atcatcgagc ggcagacgag gcacctcacc cgcctggtgg acgacctgct cgacgtctca
     5401 cgcatcatgc agggcaaggt cacgctggtc ccgcggaggg tcgagctggc agccatcgtc
     5461 gaccaggccg cggagaccgt cagacccttc atcgacgccc gcggccacac gctgacgatc
     5521 gacgtgccgc gcggcggact gcagctggag gcggacgaga cccgaatggt ccaggtcgtc
     5581 ggcaacctgc tgaacaacgc ctcgaagttc acgccggagc gagggtcgat ctcggtgtcg
     5641 gcgcgacccg atggcgacca ggtctgcctg gaggtgcgtg acgacgggat cggcatgccg
     5701 gccgagctga tcccggacgt cttcgagctg ttcgtgcagg gacggccgag cctcgatcgc
     5761 tcgcacgggg gactcggcat cggcctgacg ctggtgcgtc agctcgtgcg gctccacggc
     5821 ggcgaggtca cggccgagtc ggatggtgtc gaccgaggga gcgtcttcac ggtccgcctc
     5881 gccgccgtct ccgccccggt gccgctggac ggtgacgggg cgtccgcgcg tggcggcgcg
     5941 gaccgactcc gcgtgctggt ggtcgacgac aacgtcgaca tggccgagac gaccgcagcg
     6001 ctgctcgaga tggacggcca cgaaatcgac atcaccttcg atggtccgtc ggccgtcgaa
     6061 cgcgtcgagg cgttccagcc ctcggtcgtg ctgtgcgaca tcggcctgcc cggcatggac
     6121 ggctacgagc tggcgacgca cctgcgcgct cgctacccgg acctccggct gatcgccctg
     6181 accgggtacg gccacgacga cgcgcgtcgc gacggcgagc gcgccggctt cgaccactac
     6241 ctggtcaagc cggtcgactt ccagctgctg tcccggctcc tgcaccccag atcggcggat
     6301 cgcagcgacg gatcgtggcg cgcggacggc acggacggcg cctgaggtcg gatcctgctc
     6361 aacccgcgtc gacgacgcag aaccgattcc cctccgggtc ctccatgatc acgtagtcgg
     6421 catcggctgg ccgccggtcc cagtgcacct ctcgagcgcc gagcgatcgc agccgctcca
     6481 cctcggcccg ctggtcctcc gcgtacaggt cgaggtggat gcgcggcggc aggccgtagg
     6541 aggagtggtg ccgatccagc gagatgttcg ggccggtgcc gctccgcggc cgcatgagca
     6601 cgaagtcgtc gtcgttctcc ggccgcggca cgtagtccag cgcggcggac cagaacgccg
     6661 tcatgcgctc gaggtccgag acccggaaga cgaccgagcc gatcgagatc accgcgcgat
     6721 ctcccagatg tggccggcgg ggtcgcggaa cgcggccgtc cgaatgcccc acggtcggtc
     6781 catcggaccg ttgagcaggg tcaccccacg gcggcgcagc tcctcacacg ttgcgtcgac
     6841 gtcgctcacc tcgaccgtga gctgcgcgcg ggcgtcggtg ccggcagcgc cgaccgtggc
     6901 gggagcgatg agctcgtggg ccgccgtggt ccggagcagg ttgatgagcg tgtcgccgaa
     6961 cctgaagacc gcggactcgg catcctcgaa gaccagcggc ttgccgaaga cgtcgaggta
     7021 gaactgcttg gtggcgccga ggtcgtccac gaacagggtg atggcgccga tgcgcgccgc
     7081 ggtcagctcg tcggcctcgc gtgactcgtc gttcatgtcg tcctgcctca ctgctgcggc
     7141 gcgtgcgcgc gcgccacccg gcccatgatc ttggcaagct cccgcggccg cgaccacatc
     7201 ggccagtggc tggtcgggag gtcgatccac gtcgcgttcc ggatctccgg cagcccggcc
     7261 aggaacggcc actcggggtg ttcgcgtgcg gtctgctggt actgctcggc ggtgaagccg
     7321 gtgcagatga aggtgctcgg gatgtcgcgc cgcgcgtcgt tcgtcagctc gatctgctcg
     7381 ctcagcaccc cgcccgggac cggcagcgcc cgttcgcgga aggtggccag ctgctcctcg
     7441 ctgaggccgt cgaggttctc ctcggccgag acctcgtccc agttcatcgg gcgctcggcg
     7501 ccctcgaagt ccggctcgag tgcgcccttg cccggcgcgg tgtccacgta caccatggcc
     7561 gcgatccgtt ccggaacgcg atcgctggcg gcgtagccac tgaagcccgc cgcgctgtgg
     7621 acggccagga cgaccggcgc gtcagcggcg cgcaccgcat cgcagatggt ctcgacgtgg
     7681 tcgctcaggg tgatcgagga gcggtccgtc tcgggcgcct cgaaccccgg gagggtgagg
     7741 gccgtcacgt cgtgcccgtc ggcccggagg atcgcgacga cctcgtccca ggcccaggcg
     7801 ccgagccaga ggccgggaac gaggatgatc ggtgcggcat gggtgcgttg gttcatggtg
     7861 ctcccgtgtg cttgcgcggg tcctcgatgg tggcggatcc gctgggatgg aaatcgacgc
     7921 gcatcaccac gcgccgcttg ccggggcggc tgacctcgct catgcccgcc gcggcgaaga
     7981 tgctctcggc cccgacgtgg atctcgtccc acgtaacctc gccgacctcg cggcgcatcg
     8041 ggtaggcctc gagggcacgg gcgccacgga cgcgggcgtg ctgcacggcg gcggccgcca
     8101 gtgcgtaggc gatgccacgt ccgcggtagc cggggcggac gagcacgcac gtcacggacc
     8161 agaccgtcgg gtcgtcgcgg tcttcgccgc gaccctccca cggcgtgcgg tacacgcgtc
     8221 gcagcccgaa caggtcggcg cgcggttgca ccgcgcacca cccgaccggc tggtcgccga
     8281 ggtacgcgat cagcccggtg gtcgtggtgg cagtcggttg gcccgcatgc gtctgctcgc
     8341 gcagccgctc ggcacgctcc gaggcgggga acctggagaa ggcctcacgc ggatggagct
     8401 tgtagcgctg gcagaagcac gcggccgacg cgccgcgcgt gccgaagacg gcggccacgt
     8461 cgtcccaggg cgcctcgttc gccggcacca tacggagctc gtcgccgctc acgatcggcc
     8521 cgcccagcgc aggatgcgga gcgccttgag cgtgatccac cggctcgcgg cgccgacgtg
     8581 ctcgccgagg tcgacgtgca gctgctcgtg gtacctcgtc tccagcggcc accgaccgtc
     8641 ggcaccgcgc ttcgactcga cgacgtccag ggcctcgctc atgcgctcgt cggccgctgc
     8701 gccggatgct cggaggtagt cgaggacgcg caccacgtcg aactcccacg agtacgggaa
     8761 ccccacctcc agccagcgat cctggccgac ctctccatcg gacagccgat gcagcaggtg
     8821 tcgcgccagc aggtagtcct cgccccgctc gcgtgccgcg gcgacagccc cgtcaccgcc
     8881 ggacgcgcgc tcgaactcgc gcaacccctc caggacgttg atggtggtgt cgaaggatcc
     8941 gcgcgtcgag ccgttctcct gctcgcagtt ccatccgccg tcgtccatct gctcgttcag
     9001 cagccgatcg acgatgccgc ggacgtcgcg gccccaggca gcgcccatgc cgacgacccg
     9061 gccgttgatg cacggctcca cttcgccatc gaagaagcgg aagttcgggt catagtccca
     9121 gcgtgccgcc tcgtggacgg gctccagcgc gcgctgcagc tccgggctct ggggctcgat
     9181 gcccaactgg tggagcaggt ggagcgtgtc gaaggtgccg gtcccccgcg ggaagaaggt
     9241 gccaccgccc catcggccgt cggcgtcctg ggcggccagc agcgcggccc cccatccttc
     9301 gcgcgccacg cgcgcgcgtt cggccgccac ctcggtggcg ggcgcatcgg tcagatcgcg
     9361 aaggacctgc cagcgaatgg caggatcggc gtcgaggagc cagttcgtca cgtgcatgcg
     9421 acccatgcta cggaccgatg cgacatcaat tgtcatgatt tccggccggc gccgggatcc
     9481 tgtgcatacc gtccgcgtcc ggtctagggt ggctccatga gcgattccac catcagaccg
     9541 atcactccgc gggccttcga agacgcccac gggatggacg actggcgcgt cctcggcgac
     9601 ggcgccgcct gcttcttccg cacggcctcc ttccccgagt cggcgcggtt cgtcgcggcc
     9661 ctcgccgccg acgccacgat cgcgggccac ccaccggccg tcgacgtccg tcacgacggc
     9721 gtgacgctgc gcctcgtcac ggccagcgag gaccacttcg gcatgacgga gctcgacgtc
     9781 gaggtggcac gggccatcgg cgcaatcgct cgccgactcg gcctgacggc ggacgcgtcg
     9841 gtgctgcaga gcatcctggt cgtccccggt gcaccggaca ccgcggcggt gttcccgttc
     9901 tggcgtgagg tgctcgggta ccagccgcgg cccgacagcc cggaggagga cctcgtcgat
     9961 ccgggcgacc gcggaccggg cttctggttc gaggccatgg acgagccgcg cggcgacggc
    10021 gggggagcga tccatatcgg cgtcttcgtg ccgcacgagg tcgccgagtc ccgcgtgcac
    10081 gcggcactgg cggcgggggg ccggatggtg cgcgatgcgt acgccccgca ctggtggacg
    10141 ctcgccgatg ccgccggcaa cgaggccgac atcggcgtga tccgcgaccg cgaatagggg
    10201 tcgcggcgct accgtcgcgc gtaggagagc gcgacgacgc ctgacgcgaa ccgcttcacc
    10261 tcctgcagcc gatacgcgag cggcgcctcc agcgacggcc agaagcgcag gccgcccccc
    10321 aggacgaccg ggtggacgac cagctcgacg tcgtcgacca gcccgtggcg caggaactgc
    10381 gaggcgagat ccggcccgcc gacctcgagg tcgccgtcga gctcgcggcg cacctcggcg
    10441 aggacctccc ggacgtcgcc ccgcaccagg cgcgagttgt gctcgacgcc ctccagcgtg
    10501 gtggagaaga cgaaccgcgg cgtcgcattc cagatccgcg cgaactcccg catgacgtcc
    10561 gtcgactgcg ggtcgtcctc gccggtgggc cagtacgcgg tcatgacctc gtacaggcga
    10621 cggccgtaca ggctgccggc gagcgtccgc gcccggtcgt tgaaccactg gtgcagctcg
    10681 tcgtcgatga gcccccagtc gaggccaccg gccgcatcgg ccacgaagcc gtcgagcgag
    10741 acgttcatgg cgtagcggac ccggcccgtg gtcacccctc gccctcgagg acggcgcgca
    10801 gggtggaggc gaactcgtcc ggcttgccgg tctgcccgta ctcgccgccc aggaagccgg
    10861 cgtggttgcc ggggaagacc gtcggctcca ggcccaggcg ctcggcgatg gcgacggtcg
    10921 accggccggt cagggttcgc agcgattcct cgccggctgc catgacgatc cgcgtcgggc
    10981 cgctccggag cgcgtcgaag tcggggacgt actccgggct cgactccatg ttctggccga
    11041 ggagcggatc gtcgcgcgag ccgtcgtcct cggtcgggag gccgaacatg gccgggtccg
    11101 gcgcgggctg cgaggcccag tcgtccggga gcggcccctc gtgcatcacg agcgtaatga
    11161 accgggccat gccgacgccg aagccgtagc gctggtagtc cgcgtgcacc tggcgcgagg
    11221 cggccttggc ggcctcacgg tcgggcagca cgtcgatgat cggcggctcg tgcgccacga
    11281 gggtacggac atcgtgcggg tgcgcacgca ccagcgccat ggcattcacg gcgccgccgc
    11341 tcgtcgcgaa caggtccacc ggccccaggc cgaccgcctg gatgagccga tgcaggtcat
    11401 cggcgtgctc ctcgggcgtc gacgggccgg tcgcgtccag cttggtgctg cgctccgagc
    11461 cgcgggggtc gtacgtcacg accgtgcgat ccgggaagtg gctggccagc gtggtgaagc
    11521 ccgcggcggc catcggtgag ccgatcagga acagcggcgg ctcgctgctc ccagggttga
    11581 ggcggacgtc gtaggtcaac gtcgccccgg ggacgtcgag cgtgcgggtc gtcggctcgg
    11641 tatgggtcag ggtcatctgg gtctcctcgg ctcggcggtg aaggggtgcg atgtcactac
    11701 gacgaacggc gcggccgaat ctgatcggcg cggcggcgcc taggcgcgcg ccgcggccgg
    11761 atcctcggcg aggatggcgt cgatctgtcc cagcgcctgc ttcatccctt cctcctggcc
    11821 catggcgaga agctgctcca tcgcctcggt gctcgggaag atgttgtaga tcgacatgcg
    11881 cgtgcgccca ccgccgatct cctcgatcgc gacgcgcatc tcgcccgcgg gcatcgtctc
    11941 gttcgggctg ccgtcgtcgt tggcgaagcc gtcgcggacg accagcgacc gcggtggatc
    12001 ggtctcgagc acgtcccagt agccacgcgg ctggtcgccc tctggtcccg tcatgtggta
    12061 ctcgacgcgg ctgcccggcg tgagctcgtg cctggtgaag gtggccgggt aggtcggcgg
    12121 tccccaccag cgctccagct ggcgcggatc ggcccagagc tgccacaccc gctccggtgt
    12181 ggcgtcgaac tcggcttcca cggtcatggt cagtgcctgg ggatccttgg tgacggcggt
    12241 gacgggcatc agtcggcctc ctgtggggtg ggggagtcgg cgatgagcgc ggccatgcgg
    12301 tcgatccggc cgcgccacag ctcctcgtac tggtccagca gacggcgcgc cgcgatcagc
    12361 ccggccaggt tggtccgcac gaccttccgt cgaccgatgc gctgcttggt gaccaacccg
    12421 gcccgctcga gaatcgccac gtgcttctgc actgcggcga agctcatcgg gtagtgactg
    12481 gccagctcgg cgacgccctc
//
LOCUS       DASTYP010000134         8206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_410484, whole genome shotgun sequence.
ACCESSION   DASTYP010000134 DASTYP010000000
VERSION     DASTYP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8206
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410484"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /gene="rplP"
                     /locus_tag="VFV59_06190"
     CDS             <1..273
                     /gene="rplP"
                     /locus_tag="VFV59_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU5325457.1"
                     /translation="AMTHHIKRGGRVWIRVFPDKPVTKKPAEVRMGSGKGAPDHWVAV
                     VKPGRVMFEMSGVPEPVAREAMRLASHKLPISTKFVMKEGLDHGHQ"
     gene            260..487
                     /gene="rpmC"
                     /locus_tag="VFV59_06195"
     CDS             260..487
                     /gene="rpmC"
                     /locus_tag="VFV59_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU5325458.1"
                     /translation="MDISEVRALSDKDLEGALRDAKQELWKIRFDLATRQESNYSRLP
                     ATRKRIARILTVMTERRHAAEAEATQTEKAS"
     gene            487..771
                     /gene="rpsQ"
                     /locus_tag="VFV59_06200"
     CDS             487..771
                     /gene="rpsQ"
                     /locus_tag="VFV59_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU5325459.1"
                     /translation="MQGKRRTKTGRVVSDKMDKTVVVSVERLRRHPIYKRVVRLSTKF
                     KAHDAENTAHVGDTVRIEESRPLSRDKRWTVVEVLSRGSGEELVDGTEQA"
     gene            768..1136
                     /gene="rplN"
                     /locus_tag="VFV59_06205"
     CDS             768..1136
                     /gene="rplN"
                     /locus_tag="VFV59_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU5325460.1"
                     /translation="MIRQYSRLRVADNSGARTLMCITVLGRSTSDTAEVGDVIVASVK
                     QALPNSGVKKGDVVRAVVVRTTKEYGRPDGSHIRFDENAAVLINAQGSPRGTRIFGPV
                     ARELRERNYMKIVSLAPEVL"
     gene            1139..1468
                     /gene="rplX"
                     /locus_tag="VFV59_06210"
     CDS             1139..1468
                     /gene="rplX"
                     /locus_tag="VFV59_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5325461.1"
                     /translation="MNVRRGDTVLVLAGKDRGKRGTVERVEKTKRGYGVVIPGVNMAK
                     RHQRPRTRTQQAGIIDLPVPIHISNVQVVCPRCNKPTRVGHQQLEDGRRVRVCKQCGE
                     QIEVTTK"
     gene            1609..2142
                     /gene="rplE"
                     /locus_tag="VFV59_06215"
     CDS             1609..2142
                     /gene="rplE"
                     /locus_tag="VFV59_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5325462.1"
                     /translation="MRQRYHEEIVPALQREFEYANPMQIPRLDKVVVNIGLGEAIANA
                     KALDAAVGDLTTITGQKPIVTRAKRSIAQFRLRTGMPIGAKVTLRGQRMYDFLERTLS
                     LALPRIRDFRGIPTRSFDGRGNFSLGLREQLVYPEIDYDKIDRLRGLEISIVTTAKTD
                     EEGRKLLELLGMPFQSA"
     gene            2163..2348
                     /locus_tag="VFV59_06220"
     CDS             2163..2348
                     /locus_tag="VFV59_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU5325463.1"
                     /translation="MAKKSMIAKSQRKNRFEVREHNRCSICGRPRAYMRRFAMCRICF
                     RERALLGQLPGVTKSSW"
     gene            2458..2856
                     /gene="rpsH"
                     /locus_tag="VFV59_06225"
     CDS             2458..2856
                     /gene="rpsH"
                     /locus_tag="VFV59_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU5325464.1"
                     /translation="MNVTDPIADMLTRIRNASAARHKELSLPSSRVKREIARILAEEG
                     FIDSYETQPDGVQELLTVRLKYVEGRTPVVTGLKRISKPGLRVYARKTEIPRVLGGLG
                     LAILSTSRGIMTGSDARKQNLGGEVLCYVW"
     gene            2869..3414
                     /gene="rplF"
                     /locus_tag="VFV59_06230"
     CDS             2869..3414
                     /gene="rplF"
                     /locus_tag="VFV59_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU5325465.1"
                     /translation="MSRIGRLPISLPGGVEVTQEGSRLRVKGPLGTLEREIHPEMTLE
                     REDGTLRVVRPTDEPRHRALHGLTRTLINNMVTGVTTGFTKNLEISGVGYRAQLQGQK
                     LVLALGYSHPVEVDPPAGIEFRLESPTRLGVFGADKELVGQTAAYIRSQRKPEPYKGK
                     GIKYAGEHILRKAGKAGKVGG"
     gene            3423..3776
                     /gene="rplR"
                     /locus_tag="VFV59_06235"
     CDS             3423..3776
                     /gene="rplR"
                     /locus_tag="VFV59_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU5325466.1"
                     /translation="MTNLSRDVLRRKRHERLRLRITGTPERPRLSVFRSAKYIYAQVI
                     DDSTGRTLAAASSRESELGGSTKVDSARAVGRALAERAKAAGVEAVVLDRGGYQYHGR
                     VRSLAEGAREGGLNL"
     gene            3793..4290
                     /gene="rpsE"
                     /locus_tag="VFV59_06240"
                     /pseudo
     CDS             3793..4290
                     /gene="rpsE"
                     /locus_tag="VFV59_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            4370..4600
                     /gene="rpmD"
                     /locus_tag="VFV59_06245"
     CDS             4370..4600
                     /gene="rpmD"
                     /locus_tag="VFV59_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU5325467.1"
                     /translation="MAGKAKQSGALRVTLVRSTIGHNAGARGTVRALGLHRLNQTVEV
                     SDTPENRGMLRRVAFLLEVEEPAGSAQEEDTK"
     gene            4597..5019
                     /gene="rplO"
                     /locus_tag="VFV59_06250"
                     /pseudo
     CDS             4597..5019
                     /gene="rplO"
                     /locus_tag="VFV59_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004888697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            5208..6494
                     /gene="secY"
                     /locus_tag="VFV59_06255"
     CDS             5208..6494
                     /gene="secY"
                     /locus_tag="VFV59_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU5325468.1"
                     /translation="MIETVATALRAPDIRRKILFTLGILLVFRALTNVPVPNVNTAAL
                     TDLFTSNPLLSILNIFSGGGLAAASIIGLGVNPYINASIIMQLMRGVIPSLEELSREG
                     EYGRNKLNQYTRLLTVPLALAQGYGISVLLSANGVIPSSPLFSIETISLLLSFTAGTI
                     LLMWLGELISEKGLGNGISFIIFAGIVGSIPQQVGPTLSAPDAIGRIIPFAILGVLII
                     AAVVFINEGQRRIPVQYASRVRGRRMYQGQTQYLPLKVTMAGVIPIIFAVSILLFPAQ
                     IAAYFTTSEIDWVRNVSTWISTNLNGTNNLLLYGSLYFLLVVFFTYFYTAFQFRPDQT
                     ADYLRKNGGFIPGIRPGKPTEEFLGRVTNRITLGGALFLGIIAVLPYILTAIIPGTNN
                     LQLGGTSILIVVSVVVETMKQLEAQMMMRHYEGFIR"
     gene            6491..7186
                     /locus_tag="VFV59_06260"
     CDS             6491..7186
                     /locus_tag="VFV59_06260"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEU5325469.1"
                     /translation="MTDAPAGRHGDRLILLLVGAVGAGKGTQASILSDELGIPHLASG
                     NLFRAAMAAGSPLGEEARTYMQRGELVPDEITIGMFMEELARPEAREGAILDGFPRTV
                     GQARALDETLASQGEEIRRVFYIEVPVEELVRRVAGRWICPQCGTPYHETGDPPRVAG
                     ICDNDGTPLRQRDDDRPEVVRARLDRQVPPMLAVVEHYEAAGKVDRLDGTQPIEAVTG
                     AILARLGQPEAAY"
     gene            7186..7968
                     /gene="map"
                     /locus_tag="VFV59_06265"
     CDS             7186..7968
                     /gene="map"
                     /locus_tag="VFV59_06265"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU5325470.1"
                     /translation="MGMRSAGAATIKRSSEIDRMRAAGQILAGILEVLRAEVRPGVST
                     GELDAIAERMMRDAGAVPSFKGYGSNPPFPGVICASINDEVVHGIPSSRRRIADGDVV
                     SIDVGCIVDGWHADCARTWIVGDVPPEVTELVDATRRGMEAGIAAAVPGNRLGDVGYA
                     IESVAHERGYGIVRPFVGHGIGRSMHEEPQVPNYGRPGTGMLIEAGMCFAIEPMFTLG
                     GDDVYVADDNWTVRTADGELAAHFENTIAVTEHGPDLLTERP"
     gene            8094..>8206
                     /gene="infA"
                     /locus_tag="VFV59_06270"
     CDS             8094..>8206
                     /gene="infA"
                     /locus_tag="VFV59_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5325471.1"
                     /translation="MPKKDAIEVEGEVILPLPNAMFKVRLENGHEVLAHISG"
BASE COUNT         1411 a         2850 c         2846 g         1099 t
ORIGIN      
        1 gcgatgaccc accacatcaa gcgcggtggc cgcgtctgga tccgggtctt cccggacaag
       61 ccggtcacca agaagcccgc cgaggtccgc atgggctccg gcaagggtgc ccccgaccac
      121 tgggtcgcgg tcgtcaagcc gggccgggtg atgttcgaga tgtcgggcgt tccggagccg
      181 gtggcccgcg aggcgatgcg cctggccagc cacaagctcc cgatcagcac caagttcgtg
      241 atgaaggaag ggctcgacca tggacatcag tgaggtccgc gccctctccg acaaggacct
      301 ggagggtgcg ctgcgtgacg cgaagcagga gctgtggaag atccgcttcg acctcgcaac
      361 gcgacaggag agcaactaca gcaggctgcc ggcgacccgc aagcgcatcg cccgcatcct
      421 gaccgtgatg accgagcgcc gccacgccgc cgaggccgag gcgacgcaga ctgagaaggc
      481 cagctgatgc agggcaagcg acgaacgaag accggacgcg tggtgtccga caagatggac
      541 aagaccgtcg tggtcagcgt ggagcggctg cgacggcacc cgatctacaa gcgggtcgtg
      601 cggctctcga cgaagttcaa ggcccacgac gccgagaaca cggcgcacgt cggcgacacg
      661 gtgcgcatcg aggagagccg cccgctctcc cgtgacaagc gctggaccgt ggtcgaggtg
      721 ctgtcgcgtg gctccggcga ggagctggtg gacgggacgg agcaggcatg attcggcagt
      781 acagcaggct gcgcgtggcc gacaactccg gcgcgcggac gctgatgtgc atcaccgtgc
      841 tgggtcgctc gacgagtgac acggcggagg tgggcgacgt gatcgtcgcc agcgtcaagc
      901 aggccctccc gaactccggc gtcaagaagg gcgatgtcgt gcgcgccgtg gtggtgcgaa
      961 ccaccaagga atacggccgc ccggatggca gccacatccg cttcgacgag aacgcggccg
     1021 tcctgatcaa cgcgcagggc agcccgcgcg ggacccggat cttcggtccg gtggcccgcg
     1081 agctccggga gcgcaactac atgaagatcg tctcgctcgc cccggaggtg ctctagcgat
     1141 gaacgtacga cggggcgaca cggtgctcgt gctggcgggc aaggaccgcg gcaagcgggg
     1201 caccgtggag cgtgtcgaga agaccaagcg cggctacggg gtcgtcatcc cgggcgtcaa
     1261 catggcgaag cgccatcagc gcccgcggac ccgcacccag caggccggaa tcatcgacct
     1321 gccggtgccg atccacatca gcaacgtcca ggtggtctgc ccgcgctgca acaagcccac
     1381 gcgcgtcggc caccagcagc tcgaggacgg gcggcgggtg cgcgtctgca agcagtgcgg
     1441 cgagcagatc gaggtgacca ccaagtgagc cccgacgaga agaagccgac ccgcgcgcgc
     1501 ggcgccacga ccaagggcgc aacgagcgag gcgtcgacca acggcgagga gaaggcgacg
     1561 aacgatcgcg ccgcgaacgc cgcgtaccgg gcgccgtcgt actccgggct gcgccagcgc
     1621 taccacgagg agatcgtgcc ggcgctgcag cgcgagttcg agtacgccaa cccgatgcag
     1681 atcccgcgcc tcgacaaggt cgtggtcaac atcggcctgg gcgaggccat cgccaacgcc
     1741 aaggcgctgg acgccgcggt cggggacctg accaccatca ccggccagaa gccgatcgtg
     1801 acgcgtgcca agcgcagcat cgcgcagttc cggctccgca ccgggatgcc gatcggcgcc
     1861 aaggtgacgc tgcgcgggca gcgcatgtac gacttcctcg agcggaccct gtcgctcgcg
     1921 ctgccgcgca tccgcgactt ccggggcatc ccgacccgct cgttcgacgg ccgcggcaac
     1981 ttcagcctcg gcctgcgcga gcagctcgtg tacccggaga tcgactacga caagatcgat
     2041 cgcctgcgcg ggctcgagat cagcatcgtg acgacggcga agaccgacga ggaggggcgc
     2101 aagctcctcg agctcctcgg catgccgttc cagagcgcct gaaaggacga caggacgagc
     2161 ccatggccaa gaagtcgatg atcgcgaagt cgcagcgcaa gaaccggttc gaggttcgag
     2221 agcacaaccg ctgctcgatc tgcggacggc cgcgcgcgta catgcggcgc ttcgcgatgt
     2281 gccgaatctg cttccgggag cgcgccctgc tgggccagct gccgggcgtg acgaagtcga
     2341 gctggtaggg accggctcgt tgacgagggt ccggacccgt ccgggccgcc atcagcggaa
     2401 ggcgccaccc gcacatgggc gggagcgaac caccgccgag gagacgaacc acacgagatg
     2461 aacgtgaccg atccgatcgc cgacatgctg acgcgcatcc gcaacgccag cgcggcgcgc
     2521 cacaaggagc tgagcctccc atcgtcccgg gtgaagcgcg agattgcgcg catcctggcc
     2581 gaggaggggt tcatcgactc gtacgagacg cagccggacg gagtccagga gctgctgacc
     2641 gtccgcctca agtacgtcga gggccgcacc cccgtggtga ccggcctcaa gcgcatcagc
     2701 aagccgggcc ttcgcgtcta cgcgcgcaag accgagatcc cccgcgtcct gggcggactc
     2761 ggcctggcca tcctctcaac ctcccgcggg atcatgacgg gcagcgatgc ccggaagcag
     2821 aacctcggcg gcgaggtcct ctgctacgtc tggtaaggag cgaaacgcat gtctcgaatc
     2881 ggacgactgc cgatctcgct ccccggtggc gtcgaggtca cccaggaggg gagccgcctg
     2941 cgcgtcaagg gcccgctggg cacgctcgag cgtgagatcc acccggagat gacgctcgag
     3001 cgagaggacg gcacgctgcg cgtggtccgg cccaccgacg agccgcgcca ccgggcgctg
     3061 cacgggctga cccgcacgct catcaacaac atggtcaccg gcgtcaccac cggcttcacc
     3121 aagaacctcg agatcagcgg cgtgggctac cgcgcgcagc tgcaggggca gaagctggtg
     3181 ctcgcgctcg ggtactcgca tccggtcgag gttgatccgc cggccggcat cgagttccgg
     3241 ctcgagagcc ccacccgact gggcgtcttc ggcgccgaca aggagctggt gggccagacg
     3301 gccgcctaca tccgctcgca gcgcaagccg gagccgtaca agggcaaggg catcaagtac
     3361 gccggtgagc acatcctccg caaggccggc aaggccggca aggtcggagg ctgaggagac
     3421 cgatgacgaa cctgtctcgc gacgtcctgc gtcgcaagcg ccacgagcga ctccggctcc
     3481 ggatcaccgg aacgccggag cgcccgcgcc tgtcggtctt ccgcagcgcg aagtacatct
     3541 atgcccaggt catcgacgac agcaccggcc ggacgctggc cgcggcctcg agccgcgagt
     3601 cggagctggg cgggtcgacc aaggtcgatt cggcacgcgc cgtcggccgc gccctcgccg
     3661 agcgggcgaa ggccgcgggc gtcgaggcgg tcgtgctcga ccggggcggc taccagtacc
     3721 acgggcgggt ccgctcgctc gccgagggcg cccgcgaggg cggcctcaac ctgtagcgag
     3781 aggagacagc gcatggcacg gatcgacccg tccaagctca acctggaaga gaaggtcgtc
     3841 cagatcaacc gcgtcgccaa ggtcgtgcag ggcggtcgcc gcttcagctt cagcgcggtg
     3901 gtggtcgtcg gggacggcaa cggcaacgtc ggcgccggca tcggcaaggc cggcgaagtg
     3961 cccgaggcga ttcgcaaggg cgtcgaggac gcgaagaagc acctgatcaa ggtgccgctc
     4021 gtcggcagca ccatcccgca cgaggtgcac cggcatttcg gcgcggccaa ggtcatgctg
     4081 aagccggcca gcccgggcac gggcgtcatc gccggtggct ccgtccgctc ggtcgtcgag
     4141 gcggccggca tccgggacct gctcagcaaa gcccagggct cgaccaaccc gatcaacgtc
     4201 gtccgcgcca cggtggagtg cctcagagcg ctgcgctcgg cggacgacat cgccgccctg
     4261 cgcggccgca ccgccgagga cctgctcggc aagcgcggcg cggaggcagc gcgccggccg
     4321 cacgagatcc ccgagcggcg cgagccgatg aacgtcggag gtgaccgccg tggccggtaa
     4381 ggcgaagcag agcggcgcgc tgcgcgtgac gctcgtgcgg tcgaccatcg gccacaacgc
     4441 cggcgcgcgt ggcaccgtcc gggcgctcgg cctgcatcgg ctcaaccaga ccgtcgaggt
     4501 ctcggacacg cccgagaacc gaggcatgct gcgtcgcgtc gcgttcctcc tcgaggtgga
     4561 ggagcctgcc ggctcggcac aggaagagga caccaagtga agctccacga tctgcacccg
     4621 gctcccgggt cgcgcaagcc ggcgcgtcgc gtcggccgcg gccacggctc ggggcgcggc
     4681 aagaccgcgg ggcgcggcac gaaggggcag aagtcccgtg ccggctccag catcccggcc
     4741 tggttcgagg gtggccagac gccgctccac gtccgcacac cgaagctcca tggcttccgg
     4801 aaccgcggcc ggatcgccta cgcgccgctg aacttggcca agctggccgg ggtcgagaag
     4861 ggcacgctgg tcacgcctga cgtgctggcg cacgacgggc tgatccgcga cacgaagctg
     4921 ccggtgaagg tgctcggcaa cggcgacgcg ccgtcgggca tcacgatcca cgcgcacgcc
     4981 ttcagccgca ccgcgctcga caagctcacc gcggccggct gcacggtcca gcgcctcagc
     5041 tggccggaca acgcgccggt tgacgaggac gagcccgtcg cggaggccgt ggtgggcgag
     5101 gaggcggacg ctgccgacgt ggcggccgag ccgaccgagc cgaccgagcc gaccgacagc
     5161 gccgcggagg ccaccgagcc cgccgacagc gaaagcgaga ccgaggcttg atcgagacgg
     5221 tcgccaccgc cctccgcgcg ccggatatcc ggcgcaagat cctcttcacg ctcgggatcc
     5281 tgctggtctt ccgggcgctg acgaacgttc cggtgccgaa cgtgaacacg gccgcgctga
     5341 cggacctgtt caccagcaac ccgctgctca gcatcctgaa catcttctcg ggcggcggcc
     5401 tggcggccgc ctcgatcatc ggcctcgggg tgaaccccta catcaacgcc agcatcatca
     5461 tgcagctgat gcggggcgtg atcccgtcgc tcgaggagct ctcgcgcgag ggtgagtacg
     5521 gccgcaacaa gctcaaccag tacacccgcc tgctgaccgt gccgctggcc ctggcgcagg
     5581 gctacggcat cagcgtcctg ctcagcgcca acggcgtcat cccgtccagc ccgctgttca
     5641 gcatcgagac catcagcctg ctgctgagct tcacggccgg caccatcctc ctgatgtggc
     5701 tcggtgagct gatcagcgag aaggggctgg gcaacggcat cagcttcatc atcttcgcgg
     5761 gcatcgtggg cagcatcccg cagcaggtcg gtccgacgct gagcgcgccg gacgcgatcg
     5821 gccgcatcat cccgttcgcc atcctgggcg tgctgatcat cgccgccgtc gtgttcatca
     5881 acgaggggca gcgccggatc ccggtgcagt acgccagccg ggtccgcggg cggcgcatgt
     5941 accagggcca gacgcagtac ctgccgctca aggtgaccat ggcgggcgtg atcccgatca
     6001 tcttcgccgt cagcatcctg ctcttcccgg cccagatcgc cgcctacttc acgaccagcg
     6061 agatcgactg ggtccgcaac gtgtcgacct ggatcagcac gaacctcaac ggcaccaaca
     6121 acctgctgct gtacggcagc ctctacttcc tgctggtcgt cttcttcacg tacttctaca
     6181 cggccttcca gttccgcccg gaccagacgg ccgactacct gcgcaagaac ggcggcttca
     6241 tcccgggcat ccgccccggg aagccgacgg aggagttcct gggtcgcgta acgaaccgca
     6301 tcacgctcgg cggcgccctc ttcctgggca tcatcgccgt gctgccgtac atcctgaccg
     6361 cgatcatccc gggcacgaac aacctgcaac tcggcggcac cagcatcctg atcgtggtca
     6421 gcgtggtggt cgagaccatg aagcagctcg aggcgcagat gatgatgcgc cactacgaag
     6481 gcttcatccg gtgaccgatg cgcccgccgg tcgccacggc gaccggctga tcctcctcct
     6541 cgtcggcgcg gtcggtgccg gcaaggggac ccaggcctcc atcctgtccg acgagctcgg
     6601 catcccgcac ctcgcctccg gcaacctgtt ccgggcggcg atggccgccg gctcgccact
     6661 gggcgaggag gcgcgcacct acatgcagcg cggcgagctc gtcccggacg agatcaccat
     6721 cggcatgttc atggaggagc ttgcgcgtcc cgaggcgcgc gaaggcgcga tccttgacgg
     6781 cttcccgcgg accgtcggcc aggcgcgggc gctcgacgag accctcgcct cccagggcga
     6841 ggagatccgt cgcgtcttct acatcgaggt gccggtcgag gagctcgtcc gtcgcgtggc
     6901 cggtcgctgg atctgcccgc agtgcgggac gccgtaccac gagaccggtg acccgccgcg
     6961 cgtcgccggc atctgcgaca acgacgggac gccactgcgg cagcgcgacg acgaccgacc
     7021 agaggtcgtg cgcgcccgtc tcgaccgcca ggttcctccg atgctcgccg tggtcgagca
     7081 ttacgaggcg gccggcaagg tcgaccgcct ggacggcacc cagccgatcg aggccgtcac
     7141 gggcgcgatc ctggcccggc tcggccagcc cgaggccgcg tactgatggg gatgcgcagc
     7201 gccggtgcgg cgaccatcaa gcgcagctcg gagatcgatc gcatgcgggc cgcgggccag
     7261 atcctggccg gcatcctgga ggtgctgcgc gcggaggtcc gtccgggcgt ctccaccggt
     7321 gagctggacg cgatcgccga gcgcatgatg cgcgacgccg gcgcggtccc ctcgttcaag
     7381 ggctacggca gcaacccgcc gttccccggg gtcatctgcg ccagcatcaa cgacgaggtg
     7441 gtccacggca tcccgtcgtc gcggcgtcgg atcgccgacg gcgacgtcgt cagcatcgac
     7501 gtcggctgca tcgtcgacgg ctggcacgcc gactgtgctc ggacctggat cgtcggcgac
     7561 gtgccgccgg aggtgaccga gctggtcgac gcgacacgac gggggatgga ggcgggcatc
     7621 gcggccgccg tgccgggcaa ccggctcggc gacgtgggct acgccatcga atcggtcgcg
     7681 cacgagcgcg gctacggcat cgtgcgtccc ttcgtgggcc acggcatcgg ccgctccatg
     7741 cacgaggagc cgcaggtgcc gaactacggc cgtccgggga ccgggatgct gatcgaggcg
     7801 ggcatgtgct tcgccatcga gccgatgttc acactgggcg gcgacgacgt atacgtggcc
     7861 gacgacaact ggacggttcg aaccgcggac ggcgagctgg cggcgcactt cgagaacacg
     7921 atcgccgtga ccgagcacgg ccccgatctg ctcacggagc gaccgtgagc tgccttgtgc
     7981 ggtcggggag cgttgatgta tactcctcgt tccgtgggtc gccctgtgcg gcggcctttc
     8041 cgtgtgtcaa tcccattgat ctgatcagag cttgaaacga gggtgcgtgt cgcttgccga
     8101 agaaggatgc catcgaggtc gagggtgagg tgatcctccc cctgccgaac gcgatgttca
     8161 aggtgcgcct ggagaacggg cacgaagtgc tcgcccacat ctccgg
//
LOCUS       DASTYP010000135         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_483196, whole genome shotgun sequence.
ACCESSION   DASTYP010000135 DASTYP010000000
VERSION     DASTYP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483196"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFV59_06275"
     CDS             complement(<1..261)
                     /locus_tag="VFV59_06275"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEU5325472.1"
                     /translation="MSGRLELIALNGIPEIGPGDDLVVAIGDALDASGITLTDADVLV
                     VTQKIVSKAEGRLVDLATVEPSPFARDWAERWEKDPRQVELVL"
     gene            complement(258..860)
                     /gene="cofC"
                     /locus_tag="VFV59_06280"
     CDS             complement(258..860)
                     /gene="cofC"
                     /locus_tag="VFV59_06280"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEU5325473.1"
                     /translation="MTLRIIVPHRGLEAAKTRLAPSLDPDGRIMLASQLLQRVLRVST
                     EVTGDVVVISPSRPLREIVEASGARLEVQRGMGLNEGLDQARSQALLDGISTLVVLHG
                     DLPNLRPEDVQVLMDALPGDEAPGVAIAPDRAGTGTNGLVLRPPGVIGFRFGKGSFAL
                     HAAEAERAGVPLTAVNRAGLAFDLDTPEDLARWLKLGDAA"
     gene            complement(895..1818)
                     /gene="cofD"
                     /locus_tag="VFV59_06285"
     CDS             complement(895..1818)
                     /gene="cofD"
                     /locus_tag="VFV59_06285"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEU5325474.1"
                     /translation="MKVALLAGGTGGTKLAHGFAMLAERAAVDLTVIANVGDDTEMHG
                     LHVSPDVDALLYTLGGLMDAERGWGVRDDTHTAHAMFERYGEPTWFTIGDADLATDVV
                     RTARLRDGARLTDATAGMARALGIRARILPASDDPYRTLIETDAGTLPFQDYFVRLRQ
                     EPEVRGVRSEGADHARPTTQVLEAIEEADVVVIGPSNPIVSIGPILELPGVRQAVADA
                     GQTVAVSPIVAGRALKGPADRMLTSLGHESTALGVARIYLGVVDRFVLDEADAALAPE
                     VEALGMEAVVAPTVMRSDEDRAALARLLLGA"
BASE COUNT          287 a          751 c          744 g          293 t
ORIGIN      
        1 caggaccagc tccacctggc gtggatcctt ctcccaccgc tcggcccagt cccgcgcgaa
       61 cggcgatggc tccacggtgg ccaggtcgac cagccgtccc tccgccttgg acacgatctt
      121 ctgggtgacg accaggacgt cggcatcggt cagggtgatg ccggacgcgt cgagcgcgtc
      181 accgatggcg acgacgaggt cgtcgcctgg tccgatctcg gggatgccgt tgagcgcgat
      241 cagctcgagg cgaccgctca cgccgcgtcc ccgagcttga gccatcgcgc caggtcctcc
      301 ggcgtgtcga ggtcgaaggc gaggccggcg cggttcacgg cggtgagtgg taccccggca
      361 cgctccgcct ccgccgcatg cagcgcgaag ctgcccttgc cgaaccggaa gccgatgacg
      421 ccgggtggcc gcagcaccag cccgttcgtg ccggtgccgg cgcggtccgg ggcgatcgcc
      481 acgcccggcg cctcgtcccc cggcaacgcg tccatcagca cctgcacatc ctccggacgg
      541 aggttcggca ggtcgccatg gagtaccacg agcgtggaga tgccgtccag cagcgcctgg
      601 ctgcgcgcct ggtcgaggcc ctcgttgagg cccatgccgc gctgcacctc cagcctcgca
      661 ccggacgcct cgacgatctc gcgcagcggc cgcgacggcg agatgacgac gacgtccccg
      721 gtcacctcgg tggagacccg caggacgcgc tgcagcagct gactggcgag catgatccgg
      781 ccgtcggggt cgaggctggg ggcgagccgg gtcttcgccg cctcgagccc gcggtgcggc
      841 acgatgatcc ggagggtcac gcgggcagaa taggcgatcg ggtggctcgg cggctcaggc
      901 tcccagaaga aggcgcgcga gtgcggcccg atcctcgtcg ctgcgcatca ccgtcggcgc
      961 gacgaccgcc tccatgccga gtgcctcgac ctcgggcgcg agcgccgcgt cggcctcgtc
     1021 gaggacgaac cggtcgacca cgcccaggta gatcctggcc acgccgagag ccgtcgactc
     1081 gtggccgagc gacgtcagca tccggtccgc gggacccttc aaggcccgcc cggccacgat
     1141 cgggctgacc gccacggtct gccctgcgtc ggcgaccgcc tggcggacgc cgggcagctc
     1201 caggatgggt ccgatgctga cgatggggtt ggacggaccg atcacgacga catccgcttc
     1261 ctcgatcgcc tccagcacct gcgtcgtggg gcgcgcgtgg tccgcgccct cggaccgcac
     1321 gccgcgcacc tccggctcct ggcgcaagcg gacgaagtag tcctggaacg ggagggtgcc
     1381 ggcatcggtc tcgatcagcg tccgatacgg atcgtcgctg gccgggagga tgcgggcacg
     1441 gatcccgagg gcgcgggcca tgcccgcggt cgcatcggtc aggcgggcgc catcccgcag
     1501 gcgggcggtc cgcaccacgt cggtcgccag gtccgcgtcg ccgatggtga accaggtcgg
     1561 ctcgccgtag cgctcgaaca tggcgtgcgc cgtgtgcgtg tcgtcacgca cgccccagcc
     1621 gcgctccgcg tccatcagcc cgcccagcgt gtagagcagg gcgtcgacat ccgggctgac
     1681 gtgcaggcca tgcatctcgg tgtcgtcgcc gacgttggcg atcaccgtca ggtccaccgc
     1741 cgcgcgctcg gcgagcatgg cgaagccgtg tgccagcttc gtgccgccgg tgccgccggc
     1801 caggagcgcg accttcatgc gctcagggta ggcggtccgt gcgctgcgga atgagccgga
     1861 ttcgacgcat cttgcaggac cgtgttgcag gaccgtgcgc gccacgtcgc ggattgcgcc
     1921 ggatccggcg caatagcggg tggctgggcg ttgtcgggcg gcccggcacg ctcgctcccg
     1981 ctcttccagg ggcctggcac ccggctattg cgcgcccggc ggcacatctc gcgaagcgaa
     2041 ggctggcgaa ttgcggaatg cacgccagac gcgca
//
LOCUS       DASTYP010000136         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1613099, whole genome shotgun sequence.
ACCESSION   DASTYP010000136 DASTYP010000000
VERSION     DASTYP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613099"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFV59_06290"
     CDS             complement(<1..637)
                     /locus_tag="VFV59_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5325475.1"
                     /translation="MTSGAAMASASGVRVLVVEDEQPIRDLVARYLARDAFDVAVAAD
                     GREALEVARRQRPDVVILDLNLPGLDGLEVCRQLRVFSDAYVIMLTARGEEVDRIVGL
                     SIGADDYLVKPFSPRELVARVHALLRRPRTTGTAPAGLDLDVARRRVAVDGNAVELTP
                     IEFALLAELARRPGIVVTRTEMLTAVWGSGYDDGHVMDVHIGKLRRKLGDDA"
     gene            complement(732..1121)
                     /locus_tag="VFV59_06295"
     CDS             complement(732..1121)
                     /locus_tag="VFV59_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325476.1"
                     /translation="MTIEHTTADRQRDPVCGMSVRVDVATEDGLLTEHDAHTYYFCRR
                     ACLDEFLGAPSTYVATHAHASQAAPAGTPDIDEGMRRWYDSCSCCLSDAYPDVKAALD
                     AERAANETAPAGPGICEVAEAAEPAQT"
     gene            complement(1174..1605)
                     /locus_tag="VFV59_06300"
     CDS             complement(1174..1605)
                     /locus_tag="VFV59_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5325477.1"
                     /translation="MNQKRRRILVVNDTEEILELFHDILEGMGHEMIPLTYAPDDLQQ
                     IVEIMPDLAIIDFVMGGLEYRGWQLVQKLRMHRLTANIPLIICTAAKREVYEQEGWLT
                     EKGVSIVLKPFDIEDLERAIERAFSTAEMRRTRPGVDATSR"
     gene            1734..2093
                     /locus_tag="VFV59_06305"
     CDS             1734..2093
                     /locus_tag="VFV59_06305"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEU5325478.1"
                     /translation="MNRFLRNYSLGIVLAVLFFASWILQSVTGWVEFTAEQQAHGEAA
                     QLFGASGYFWPWMQATFENWQSEFLQLFTMAILTAFLIHKGSSESKDSDDEMMLELTQ
                     IRRELIELKQAQGKARS"
     gene            2090..2287
                     /locus_tag="VFV59_06310"
     CDS             2090..2287
                     /locus_tag="VFV59_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325479.1"
                     /translation="MSKATGKEQNAELERQRKELEQALDEQIIDTMRSAALPNALAWV
                     IVVGLSFVINLLVLVAISGGG"
     gene            2292..2711
                     /locus_tag="VFV59_06315"
     CDS             2292..2711
                     /locus_tag="VFV59_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5325480.1"
                     /translation="MASLVTSAADAPGEGERSPQRPVVLVVDDSADMRTLIAEVLTPA
                     GYEVVGVPSGARALRFMADRIPDVMITDLFMPGMSGFTLRSTMLRRPDLAHIPVIVLS
                     AYWHRPAETLDAVEAIPKPINIDRLLEAVGRLTKAGE"
BASE COUNT          442 a          979 c         1030 g          457 t
ORIGIN      
        1 ctgcgtcgtc accgagcttg cgtcgcagct tgccgatgtg cacgtccatg acgtgaccgt
       61 cgtcgtaacc gctgccccac acggcggtga gcatctcggt tcgggtgacg acgatgccgg
      121 gccggcgggc gagctccgcg aggagcgcga actcgatcgg ggtcagctcg accgcgttcc
      181 cgtcgaccgc cacgcgccgg cgcgcgacgt cgaggtccag gccggccggt gccgtgcccg
      241 tcgtccgcgg tcgccggagc agcgcgtgca cgcgcgccac gagctctcgc gggctgaacg
      301 gcttgaccag gtagtcgtcc gcgccgatcg acaggccgac gatccggtcg acctcctccc
      361 cgcgggccgt cagcatgatc acgtacgcgt cagagaagac gcggagctga cgacagacct
      421 cgagcccgtc caggccaggc aggttcagat cgaggatgac gacgtcgggt cgctggcgcc
      481 tggccacctc gagcgcctct cgcccatcgg cggcgacggc gacgtcgaag gcgtcgcgcg
      541 cgaggtagcg cgcgacgagg tcgcgaatcg gctgctcgtc ttccacgacc agcacgcgca
      601 cgccggatgc gctcgccatg gcggctccac tcgtcatcgg ccgacgacgg tacgccgcgc
      661 ccaggcgtgg tcgaaggtcc gaagcgaccg aacgcgtcgg gcaagtgtcc cgccgaggcg
      721 ggacaccggg atcaggtctg cgccggctcc gcggcctccg cgacctcgca gatgcccggt
      781 ccggcgggtg ccgtctcgtt cgcggcccgc tcggcgtcga gcgccgcctt gacgtcggga
      841 taggcgtccg agaggcagca cgaacacgag tcgtaccagc gacgcatccc ctcgtcgatg
      901 tccggcgtgc ccgcgggcgc cgcctgagat gcgtgcgcgt gcgtggccac gtacgtggac
      961 ggtgcgccga ggaactcgtc caggcatgcc cggcggcaga agtagtacgt gtgcgcgtcg
     1021 tgctcggtga ggaggccgtc ttcggtggcc acgtcgaccc gaacgctcat gccgcagacc
     1081 gggtcgcgct gccgatccgc cgttgtgtgc tcgattgtca tggcgcgagc ctagcggacc
     1141 gggagacgag cgtcaccgtg acgtggttgc cagtcagcgg gacgtggcat ccacgccggg
     1201 gcgcgtccgg cgcatctcgg ccgtcgagaa ggcgcgctcg atggcccgct ccaggtcctc
     1261 gatgtcgaac ggcttgagca cgatggagac gcccttctcg gtgagccagc cctcctgctc
     1321 gtacacctcg cgcttggcgg cggtgcagat gatcagcggg atgttcgcgg tgagtcgatg
     1381 cattcgcagc ttctgcacga gctgccatcc gcggtattcg aggccgccca tgacgaagtc
     1441 gatgatcgcg agatccggca tgatctccac gatctgctgg agatcgtccg gcgcgtaggt
     1501 gagcgggatc atctcgtgcc ccatcccctc gaggatgtcg tggaacagct ccaggatctc
     1561 ctcggtgtcg ttgacgacga ggatgcggcg gcgtttctgg ttcatcggcg taggggcacg
     1621 caatcgcggt gccagcctct ccgtggtggt gcccgtcccc cggcacgccg gatgcataac
     1681 ggcggcgtca ggtgggtccc ggagggcggg accccgcgac gagaggacca cgtgtgaacc
     1741 ggttcctgcg gaactacagc ctggggatcg tgctggccgt gctcttcttc gcgagctgga
     1801 tcctccagtc cgtcaccggc tgggtcgagt tcacggcgga gcagcaggcg cacggcgagg
     1861 cggcgcagct cttcggtgca tcgggctact tctggccctg gatgcaggcg accttcgaga
     1921 actggcagtc ggagttcctc cagctgttca cgatggccat cctcacggcc ttcctgatcc
     1981 acaaggggag ctccgagtcg aaggactccg atgacgagat gatgctcgag ctgacccaga
     2041 tccggcgcga gctcatcgag ctgaagcagg cccaggggaa ggcgaggtca tgagcaaggc
     2101 gaccggcaag gagcagaacg ccgagctcga gcgccagcgc aaggagctcg agcaggcgct
     2161 cgacgagcag atcatcgaca cgatgcggtc cgcggcgctg ccgaacgcgc tggcgtgggt
     2221 gatcgtcgtg ggcctctcgt tcgtcatcaa cctcctcgtc ctggtcgcga tcagcggcgg
     2281 cggatgagcg catggcgtcg ttggtgacat ccgcggccga tgcgccgggg gagggtgagc
     2341 gatctcccca gcgccccgtg gtcctggtcg tggatgactc cgccgacatg cgcacgctca
     2401 tcgccgaagt cctgacgccc gccggctacg aggtggttgg cgtgccgtcg ggcgctcgag
     2461 ccctgcggtt catggccgac cgcatccccg acgtcatgat caccgacctc ttcatgcccg
     2521 gcatgtcggg cttcacgctg cgctccacca tgctgcggcg acccgacctg gcgcacatcc
     2581 ccgtcatcgt gctgtccgcg tactggcatc ggcccgccga gacgctcgac gcggtcgagg
     2641 ccattccgaa accgatcaac atcgaccggc tgctggaggc cgtcgggagg ctgacgaaag
     2701 cgggggaatg aactcgatgg gggatgcgca cggtggggcc gcgtgacgac gcgctgccac
     2761 gccgcgaccc gcgaggcgcg ttcagcagcg ctgagcgagc attcgagccg gggtctggca
     2821 tgctcggtgc ccatggaggc cgcatgggga ccctcacgac cgccgaacgg ccgatgcgca
     2881 cctgcgagcg ctgcgggcgc accgggta
//
LOCUS       DASTYP010000137         3565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_556323, whole genome shotgun sequence.
ACCESSION   DASTYP010000137 DASTYP010000000
VERSION     DASTYP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556323"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFV59_06320"
     CDS             complement(<1..476)
                     /locus_tag="VFV59_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEU5325481.1"
                     /translation="MSASSGAAGRPTKPRASLVVPIGLVVFVLVTWASVSEAFGIGLD
                     IGELIADITRGAGILGELLSPNWGFLPRTVEPMLETFKMAVVASAIGCGVALPVAFLA
                     SRVTAPNAATFLVDRSILSVVRAIPDILYALVFVAAVGIGPLPGIAALVLFNIGVVA"
     gene            complement(473..1258)
                     /gene="phnC"
                     /locus_tag="VFV59_06325"
     CDS             complement(473..1258)
                     /gene="phnC"
                     /locus_tag="VFV59_06325"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEU5325482.1"
                     /translation="MIRFENASVTYPGGVHALKNLTLEIPDGQMVVIVGLSGAGKSTL
                     IRAINGLVPLSDGDVTIDGVSVRHASASQLRQIRSNIGMIFQTFNLVKRTSVLNNVLM
                     GRLHDTGTLRSLLGWYKPADVEIAMQALERVGIVEKAYVRASNLSGGQQQRVGIARAL
                     AQQPKILLADEPVASLDPPTSHVVMRDLQRINRDLGITTIVNLHFLDLAKIYGERIIG
                     MRAGELVYDGTGEQANEDVFRDIYGRSLTADDLMGEATVAAAG"
     gene            complement(1314..2375)
                     /locus_tag="VFV59_06330"
     CDS             complement(1314..2375)
                     /locus_tag="VFV59_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5325483.1"
                     /translation="MKHLRLLTALVAMMIIVAACNSGQGASASAEESEAPGSQPAESQ
                     AEESQSAEMPDKLVIGFVPSREAGALVEDIQPLADYLSEQLGIPVEGVVSNDYTGLVT
                     AMETGQAQIGALPPYGMVQAIDRAGAEVILQSERFGSGTYHTQFFTNNPDKYCEDEPV
                     ENTRMVDDQEVTYLNCNGTDRANDETPEGPVGLDALANIDAGTAVSFVEQTSSSGYIF
                     PATVLLSQNIDPESDIEAVFAGSHDASVIAVCEGQAEVGVSFDDARTEATTDCDVENN
                     VVVFAYGPEIPNDGVAVSGDVPAELREQIKQALLDYADTEEGKATLEKIYNITAFTEP
                     NLDSLDIIRQAVEELGYGS"
     gene            complement(2490..2565)
                     /locus_tag="VFV59_06335"
     tRNA            complement(2490..2565)
                     /locus_tag="VFV59_06335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2530..2532),aa:Arg,seq:ccg)
     gene            2664..3401
                     /locus_tag="VFV59_06340"
     CDS             2664..3401
                     /locus_tag="VFV59_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEU5325484.1"
                     /translation="MHAWRRVNESDMPDPTQPHETRANSVLRPIVFGANDGLVSNLAL
                     VMGVAGANPEPGIIVLAGIAGLLAGAFSMGVGEYISVQSQRELLDFQIAFQRHQLREV
                     PEQERQILADIYRKRGLTDDEADHFTRRLFADPENAVRLLIFEEVGLDARSIGSPISA
                     GVSSFLAFTLGASVPLIPYLLSGGGLAFGLSLGLSLASLAILGYLISRMTRRHPLYSS
                     VRQVVLGGVAAAVTFGVGTLIGAAPGP"
BASE COUNT          534 a         1204 c         1236 g          591 t
ORIGIN      
        1 gcgacgacgc cgatgttgaa cagcaccagc gcggcgatgc cgggcagcgg cccgataccc
       61 accgcggcga cgaagaccag cgcgtagagg atgtccggga tggcgcggac gacgctgagg
      121 atggaccgat cgaccaggaa cgtcgccgcg ttcggcgccg tgacgcgcga cgcgaggaag
      181 gccaccggca acgcgacccc gcagccgatg gccgacgcga cgacggccat cttgaacgtc
      241 tcgagcatcg gctcgaccgt ccgcggcagg aagccccagt tcgggctcag cagctcaccg
      301 aggatgccgg cgccgcgcgt gatgtccgcg atcagctccc cgatgtcgag gccgatgccg
      361 aaggcttcgc tgaccgatgc ccaggtgacg aggacgaaga cgacgagccc gatcgggacg
      421 acgagcgagg cgcgcggctt cgtgggccgc cctgcggcac cggacgaggc gctcatccgg
      481 cagcggcgac cgtggcctcg cccatcaggt catcggccgt gagggagcga ccgtagatgt
      541 cgcggaagac gtcctcgttg gcctgctcgc cggtcccgtc atagaccagc tcgccggcgc
      601 gcatgccgat gatccgctcg ccgtagatct tggccagatc caggaagtgc aggttgacga
      661 tggtcgtgat gccgaggtcg cggttgatgc gctgcaggtc gcgcatcacg acgtggctgg
      721 tcggcggatc caggctggcg accggctcgt cggccaggag gatcttgggc tgctgcgcca
      781 gcgcgcgggc gatgccgacg cgctgctgct ggccgcccga caggttgctg gcgcggacgt
      841 acgccttctc gacgatcccg acgcgctcga gcgcctgcat ggcgatctcg acgtcggccg
      901 gcttgtacca gcccagcagg ctgcggagcg tgccggtgtc gtggagacgg cccatcagca
      961 cgttgttcag gacggacgtt cgtttcacca ggttgaacgt ctggaagatc atcccgatgt
     1021 tcgagcggat ctggcgcagc tggctggcgc tcgcgtgccg gacgctgacg ccgtcgatcg
     1081 tcacgtcacc atcggagagg ggcaccaggc cgttgatggc ccggatgagg gtcgacttgc
     1141 ccgctccgga caggccgacg atgacgacca tctggccgtc ggggatctcg agcgtgaggt
     1201 tcttgagggc gtgcacgcca ccggggtagg tgacggacgc gttctcgaag cgaatcatgg
     1261 gcggatgaag aaggggcggg cccgatcggg acccgcccct cgctcagccg agctcagctg
     1321 ccgtagccga gctcttcgac cgcctgccga atgatgtcga gcgaatcgag gttcggctcg
     1381 gtgaaggcgg tgatgttgta gatcttctcg agggtggcct tgccctcctc ggtgtcggcg
     1441 tagtcgagga gtgcctgctt gatctgctcg cgcagctcgg ccggcacgtc acccgagacg
     1501 gcgacgccgt cgttcgggat ctccggtccg taggcgaaga ccacgacgtt gttctccacg
     1561 tcgcagtccg tggtcgcctc tgtgcgggcg tcgtcgaagc tgacgccgac ctcggcctgg
     1621 ccctcgcaca ccgcgatgac cgacgcgtcg tggctgcccg cgaagacggc ctcgatgtcg
     1681 gactcgggat cgatgttctg gctcagcagg acggtcgccg ggaagatgta gcccgacgag
     1741 ctggtctgct cgacgaacga caccgcggtc cccgcgtcga tgttcgcgag cgcgtccagg
     1801 ccgacggggc cctcgggcgt ctcgtcgttc gcgcggtcgg tgccgttgca gttgaggtac
     1861 gtgacctcct ggtcgtcgac catgcgcgtg ttctcgaccg gctcgtcctc gcagtacttg
     1921 tccgggttgt tggtgaagaa ctgcgtgtgg tatgtgccac tgccgaagcg ctcggactgg
     1981 aggatgacct ccgcgcccgc gcgatcgatg gcctggacca tcccgtacgg cggcagggcg
     2041 ccgatctgcg cctggccggt ctccatggcc gtgaccagac cggtgtagtc attcgagacg
     2101 acgccctcga ccgggatccc gagctgctcg ctcaggtagt cggccagcgg ctggatgtcc
     2161 tcgacgaggg ccccggcctc acgcgacggg acgaacccga tgacgagctt gtcgggcatc
     2221 tcggccgact ggctctcctc ggcctggctc tcggcgggct ggctgccggg cgcctcgctc
     2281 tcctcggcgc tggcgctcgc gccctggccg gaattacagg ccgccacgat gatcatcatc
     2341 gcgacgagcg ctgtcagaag tcgcaggtgc ttcatgtacc tctctccagt gcgccgcatg
     2401 acttgctggg cggcgggacc gagcgttcct ccccctcgcg cacccacggc ggcgtgaatc
     2461 ggcttgcatt ctagcgtcgg gtgggggagt ggcgcgccgg acaggattcg aacctgcgac
     2521 cttcggctcc ggaggccgac gctctatcca ctgagctacc ggcgcgcgat ccgaagcggg
     2581 ggccatcata cccggccccg tcggaaccgg cccgcatgcc gatcggccct gccgcggtcc
     2641 ccagtggctg ccatcatcgg cgcatgcatg cgtggcgacg ggtgaacgag tcggacatgc
     2701 ccgatccgac gcagcctcac gagacgcgcg ccaacagcgt gctgcggccg atcgtgttcg
     2761 gcgcgaacga cgggctcgtc tcgaacctgg cgctggtcat gggcgtggcg ggcgcgaacc
     2821 cggagccggg gatcatcgtg ctcgccggca tcgccggcct gctggccggc gccttcagca
     2881 tgggcgtcgg ggagtacatc tcggtccaga gccagcggga gctgctcgac ttccagatcg
     2941 ccttccagcg ccaccagctg cgcgaggttc cggagcagga acgccaaatc ctggccgaca
     3001 tctaccggaa acgcggactg accgatgacg aggccgacca cttcaccagg cgcctgttcg
     3061 ccgatcccga gaacgcggtt cgcctgctca tcttcgagga ggtcgggctg gacgcgcggt
     3121 cgatcggctc acccatcagc gccggcgtca gctcgttcct ggccttcacc ctcggggcgt
     3181 cggtgccgct catcccgtac ctgctcagcg gcggcgggct cgccttcggg ttgagcctgg
     3241 gcctgagcct cgcctcgctc gcgatcctgg gctacctgat cagccgcatg acgcgacgtc
     3301 acccgctcta cagctcggta cggcaggtgg tcctcggggg cgtcgcggcg gccgtgacgt
     3361 tcggcgtggg gacgctcatc ggggcagcgc ccggtccgta ggacgtcccc tcaacaagcg
     3421 gaaacccccg ccgaagcggg ggtccccatt gctgccggat tcgctaatcg caagggcatc
     3481 tttcacgcac ggtttcccgc actaccctaa ccctaaccta cccatggcct tgcgaccagt
     3541 agtgaatcgc tgtgcgaaca gcccg
//
LOCUS       DASTYP010000138         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_410997, whole genome shotgun sequence.
ACCESSION   DASTYP010000138 DASTYP010000000
VERSION     DASTYP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410997"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFV59_06345"
     CDS             complement(<1..688)
                     /locus_tag="VFV59_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEU5325485.1"
                     /translation="MVLGIDVSTTATKAILVDGSGGVRGIGVAEYPYETPRPQWSEQD
                     PALWWDGTQAAIRAALAAAGVDGGDVATVGLTGQMHGSVLLDADDQPVRPAILWNDQR
                     TAAECDAIREAVGPERLIQITGNDALTGFTAPKLLWVRSHEPEAWARVTGVMLPKDYV
                     RLRLTGERAVDKADGAGTLLFDLAARDWSEEMLAALDLDPTLFPRTFEGPAVTGTVTA
                     GAAAATGLAAG"
     gene            complement(699..2042)
                     /gene="xylA"
                     /locus_tag="VFV59_06350"
     CDS             complement(699..2042)
                     /gene="xylA"
                     /locus_tag="VFV59_06350"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEU5325486.1"
                     /translation="MAPFFPDVPDRIGFGGLHSDDPFSFKVYDPDRLVRGTRMEDQLR
                     IAVCLWHSFNWPGSDVFGVGTFDRPWLSAGMEPMEAARAKLDAAFEFLEKLGVPFFTF
                     HDRDVAPEGATFAETRANLDAVAAAIESHMERTGLKLLWGTANLFGHPRYAAGAATNP
                     DPEVFAYAAAQVKVMLETTHRLGGANYVLWGGREGYETLLNTDLRREEQQLARFLTLV
                     AEHKHAIGFTGQLLLEPKPQEPTKHQYDYDVGTVDGFLVRHDLADEYRVNIEANHATL
                     AGHSFQHEVATATSRGIFGSVDMNRGDYQNGWDTDQFPNSVDELALAVYEILRGGGFS
                     GGGGFNFDAKLRRQSLDRHDLFHAHIGGIDTLARALLVAADMLETGDLEQLRTARYAG
                     WDDGIGAELLGGGVSLAEIESRVVDEGIEPRPRSGAQERLENVVNQHIWAVDRGR"
BASE COUNT          294 a          748 c          764 g          328 t
ORIGIN      
        1 tcccggccgc caggcccgtg gcggccgccg ccccggcggt gacggtgccg gtcacggccg
       61 gtccctcgaa cgtccgcggg aagagggtcg ggtcgaggtc gagcgcggcg agcatctcct
      121 ccgaccagtc gcgcgcggcc aggtcgaaca acagcgtgcc ggcgccatcc gccttgtcga
      181 cggcgcgctc gccggtgagg cgcagtcgca cgtaatcctt cgggagcatg accccggtga
      241 cccgcgccca cgcctccggc tcgtggctgc gcacccacag cagcttcggc gcggtgaagc
      301 cggtcagcgc gtcgttgccg gtgatctgga tgagccgttc gggccccacc gcctcgcgga
      361 tggcgtcgca ctccgcggcc gtgcgctggt cgttccacag gatggccggg cggacgggtt
      421 ggtcgtccgc gtccagcagc acggagccgt gcatctgccc ggtcaggccg acggtcgcga
      481 cgtcaccacc gtccacgccg gcagccgcca gcgcggcgcg gatggccgcc tgggtgccgt
      541 cccaccacag cgccggatcc tgctcggacc actgcgggcg tggcgtctcg tacggatact
      601 cggccacgcc gatgccgcga acgccgccgg atccgtcgac gaggatcgcc ttggtcgcgg
      661 tcgtcgagac gtcaatgccg aggaccatcg gcgccacgtc agcgcccccg gtcgacggcc
      721 cagatgtgct gattgacgac gttctcaagc cgttcctgcg cgccggagcg ggggcgcggc
      781 tcgatgccct cgtccaccac gcggctctcg atctcggcca gcgagacgcc gccacccagc
      841 agctcggcgc cgatgccgtc gtcccagccc gcgtagcgcg cggtgcgcag ctgctccagg
      901 tcgcccgtct ccagcatgtc ggccgccacg agcagcgccc gcgccagcgt gtcgatgccg
      961 ccgatgtggg cgtggaacag gtcgtggcgg tccaggctct ggcggcggag cttggcgtcg
     1021 aagttgaagc cgccgccacc gctgaaccca ccgccccgca ggatctcgta cacggccagc
     1081 gccagctcgt cgaccgagtt cgggaactgg tcggtgtccc acccgttctg gtagtcgccg
     1141 cggttcatat ccacgctgcc gaagatgccg cggctggtgg ccgtcgccac ctcgtgctgg
     1201 aacgagtggc cggccagcgt ggcgtggttg gcctcgatgt tgacgcggta ctcgtccgcc
     1261 aggtcgtgac ggaccaggaa gccgtcgacg gttccgacgt cgtagtcgta ctggtgcttg
     1321 gtcggctcct gcggcttggg ctcgagcagc agctggccgg tgaagccgat ggcgtgcttg
     1381 tgctccgcga ccagggtgag gaaccgcgcg agctgctgct cctcccgccg caggtcggtg
     1441 ttcaggagcg tctcgtaccc ctcgcggccg ccccacagga cgtagttcgc gccgcccagc
     1501 cggtgcgtgg tctcgagcat gaccttgacc tgtgccgctg cgtacgcgaa gacctcggga
     1561 tccgggttgg tcgcggcgcc ggccgcgtag cgcgggtggc cgaagaggtt ggccgtgccc
     1621 cacagcagct tcagcccggt gcgctccatg tgcgattcga tggccgcggc cacggcgtcc
     1681 aggttggcgc gcgtctcggc gaacgtcgct ccctccggcg ccacgtcacg gtcgtggaag
     1741 gtgaagaagg ggacgcccag cttctccagg aactcgaagg cggcgtcgag cttcgctcgc
     1801 gccgcctcca tcggttccat gccggcggac agccacggac gatcgaaggt gcccaccccg
     1861 aagacgtcgg agccgggcca gttgaacgag tgccagaggc agacggcgat ccgcagctgg
     1921 tcctccatgc gcgtgccgcg caccaggcgg tcggggtcgt agaccttgaa gctgaacggg
     1981 tcgtcggagt gcagtccgcc gaagccgatg cggtcgggga cgtcggggaa gaacggggcc
     2041 acgtcgatcc tcctgtggcg cgggatgcgg gcgtttgggt gcgctcctga cggtatcaga
     2101 agcgtttgtg aaagagaagc acaaacatgc gcca
//
LOCUS       DASTYP010000139         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_630221, whole genome shotgun sequence.
ACCESSION   DASTYP010000139 DASTYP010000000
VERSION     DASTYP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630221"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..908
                     /gene="heR"
                     /locus_tag="VFV59_06355"
     CDS             162..908
                     /gene="heR"
                     /locus_tag="VFV59_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HEU5325487.1"
                     /translation="MSTDRGLSRLRLYNLAMGLLHAAQGVAVLVLSNDFALPVTAAFM
                     EGPPGTPASTKELFTLPLGPAVAAFLFLSAIAHFVVAGPGWGKYQRDLAAHRNEQRWI
                     EYSLSSSLMIVLIAMITGIADIAALVALFGVNASMILFGWLQERYEEPGGGGWWPFVF
                     GCVAGIVPWVAVGIYLIGPSAEVPGFVYAIFVSLFVFFNSFALNQVLQYKQVGKWRDY
                     LFGERVYVALSLVAKSALAWQVFANTLVPS"
     gene            complement(927..1532)
                     /locus_tag="VFV59_06360"
     CDS             complement(927..1532)
                     /locus_tag="VFV59_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5325488.1"
                     /translation="MPWSRHAKIAFIGSHSVRKSNAVHAFASTVGRAGRSVEVGREVV
                     RFNPLGLNEGATPQAQLWVLMAQVQQELELASRAEVLVTDRSVVDNFAYYLRVTGGGD
                     PFGVEPLIRSWAGTYDLSVRLLPDVALRPDGVRSTSDAFRDEIEGILDRILPDYLGPE
                     RLLTIGASQVTADFDWWSIAERLAALVAEPLVEGQLRAPLA"
     gene            complement(1542..2717)
                     /locus_tag="VFV59_06365"
     CDS             complement(1542..2717)
                     /locus_tag="VFV59_06365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEU5325489.1"
                     /translation="MNPLDLVVLLLLALGLFSGARAGFVSPVLGLAGAVIGFGLALLA
                     ASVFRDSLGQIEQPMRALVTLLGLAAFVLAGEAAGAGIGATVSFGLRRSLFRPVDALG
                     GALVGAAHVVLMVWLFAGMLNMGLLPAFGDVARDSVALRTVRSVLPAPTLVAGRLLAL
                     LDTTDLPPLFAGIEPPPAAPVDLPADAEVRTLAQSAIPSTGRVAASGCGAGQSVGSGF
                     FIAPTYLVTNAHVVAGSSATTVTVGTASHAAVVVDYDDGIDLALLHVDGVNVTPLRLM
                     ADAPGRGSTGAVLGFPGGGAMTVTAGAVTATHLVPGPNIYGEGAYDRTVVELRAEIRR
                     GNSGGPLVVEPGVVGAIIFGASRANPEVGYAIGANDALEHLGPSIGATAPADTGACL"
     gene            2812..>2987
                     /locus_tag="VFV59_06370"
     CDS             2812..>2987
                     /locus_tag="VFV59_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5325490.1"
                     /translation="MARIVRAALLQAEWTGDKESMIQKHEKYAREAAAQGAKVMCFQE
                     LFYGPYFCQVQDPQ"
BASE COUNT          446 a         1061 c         1066 g          414 t
ORIGIN      
        1 ccgcgcctcc ggcgacggcc cggcgaccgg caaccgcggc agcgcggcct gatccacccg
       61 ggacgcgccg tcgtatcgag acgtggggcc gattggccct cgccgctgct ctcgcgtgtg
      121 cggagcatcg gtgcagaacc atcgaaacca ccgagggcgc catgtccacc gatcgaggcc
      181 tgtcgcgcct ccgcctctac aacctcgcca tgggcctgct gcatgctgcg cagggcgtgg
      241 cggtgctggt cctgtccaac gatttcgcgc tgccggtgac ggcggcgttc atggagggtc
      301 cgccgggcac gccagcgagc acgaaggagc tcttcacgct gccgctgggc ccggccgtcg
      361 ccgccttcct gttcctctcg gccatcgccc acttcgtggt ggccggtccg ggctggggca
      421 agtaccagcg agacctcgcc gcccaccgca acgagcagcg ctggatcgaa tactcgctca
      481 gctcgtcgct gatgatcgtg ctcatcgcca tgatcaccgg catcgcggac attgccgcgc
      541 tggtcgcgct gttcggcgtg aacgcctcga tgatcctgtt cggctggctc caggagcgct
      601 acgaagagcc cggcggcgga ggctggtggc cgttcgtgtt cggctgcgtc gcggggatcg
      661 tgccgtgggt cgcggttggg atctacctca tcggtccgtc cgccgaggtg cccggcttcg
      721 tgtacgcgat cttcgtgtcg ctgttcgtct tcttcaactc gttcgcgctc aaccaggtcc
      781 tgcagtacaa gcaggtcggg aagtggcgcg actacctgtt cggtgagcgc gtctacgtgg
      841 cgctgagcct ggtcgccaag tctgcgctgg cctggcaggt cttcgcgaac accctcgtcc
      901 ccagctgagg ccgatgcgcc gcgcgctcag gcgagtggcg cgcgcagctg cccctccacc
      961 agcggctcgg cgaccagggc ggcgagccgc tccgcgatcg accaccagtc gaagtcggcg
     1021 gtgacctggc tggcgccgat ggtcaggagc cgctctgggc ccaggtagtc cggcaggatg
     1081 cggtcgagga tgccctcgat ctcgtcacgg aaggcgtcgc tggtcgagcg gacgccgtcc
     1141 ggccgcagcg ccacgtcggg cagcagccgg accgacaggt cgtacgtccc ggcccagctg
     1201 cggatcagcg gctccacgcc gaacgggtcc ccgccgccgg tgacgcgcag gtagtacgcg
     1261 aagttgtcga cgacggaacg atcggtgacc agcacctcgg cgcgagaggc gagctcgagc
     1321 tcctgctgca cctgggccat caggacccag agctgcgcct gcggcgtcgc accctcgttg
     1381 agtcccagcg ggttgaagcg gaccacctcg cgtcccacct cgaccgagcg gccggcacgc
     1441 ccgaccgtcg aggcgaacgc gtggacggcg ttcgacttgc gcacggagtg cgagccgatg
     1501 aaggcgatct tggcatggcg cgaccagggc accgccgcag cctacaggca ggcgccggtg
     1561 tcggcgggcg ccgtggcacc gatggacggt cccaggtgct cgagcgcgtc gttggcgccg
     1621 atggcgtagc cgacctcggg gttcgcgcgg gaggcgccga agatgatcgc ccccaccacc
     1681 ccgggctcca cgaccagcgg gccgccgctg ttcccgcgcc ggatctccgc ccgcagctcg
     1741 accaccgtgc ggtcgtacgc tccctcgccg tagatgttcg gccccggcac gaggtgcgtc
     1801 gccgtcaccg cgccggccgt gaccgtcatg gcgccgccgc ccgggaagcc gagcactgcg
     1861 ccggtcgagc cccggccggg cgcgtcggcc atgaggcgca gcggcgtgac gttcacgccg
     1921 tcgacgtgca ggagcgccag gtcgatcccg tcgtcatagt cgacgaccac ggccgcgtgt
     1981 gacgccgtcc cgacggtcac cgtggtggca gagcttcccg cgaccacgtg cgcgttggtg
     2041 acgaggtacg tcggagcgat gaagaacccg ctccccacgg actggccggc cccgcagcca
     2101 ctggcggcga cgcgtcccgt gctggggatg gcggactggg ccagggtccg gacctcggca
     2161 tcggccggga ggtcgaccgg ggccgcgggc ggtggctcga tcccggcgaa gaggggcggc
     2221 aggtccgtcg tatcgagcag cgcgagcaga cggccggcca ccagcgtggg agccggaagc
     2281 acggagcgga ccgtccgcaa cgcgacggaa tcacgcgcca cgtctccgaa ggccggcagc
     2341 aggcccatgt tcagcatccc ggcgaacagc cagaccatga ggacgacgtg cgccgcgccc
     2401 accagtgccc cgccgagcgc gtccaccggc cggaagagcg atcggcgcag cccgaagctc
     2461 accgtcgccc cgatcccggc gcccgccgcc tcgcccgcca gcacgaacgc cgccaggccg
     2521 agcagggtga ccagggcgcg catcggctgc tcgatctggc cgagcgagtc gcggaagacc
     2581 gacgccgcca gcagcgccag tccgaacccg atgacggcgc ccgccaagcc gaggaccggg
     2641 ctgacgaatc cggcgcgggc tccggagaag aggccgagcg cgagcagcag gagaacgacg
     2701 aggtcgaggg ggttcatcgg cgcggctagc gtagctgtcg gccggtgtag agtatcggct
     2761 cttcatccgg cagcccctga tctgcccgac ttgcgtaagg acggaggacc gatggcgcga
     2821 atcgtccgcg cggcactgct ccaggcggag tggaccggcg acaaggagtc gatgatccag
     2881 aagcacgaga agtacgcgcg cgaggccgcg gcccagggcg cgaaggtgat gtgcttccag
     2941 gagctgttct acggccccta cttctgccag gtccaggacc cgcagta
//
LOCUS       DASTYP010000140         6526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_630238, whole genome shotgun sequence.
ACCESSION   DASTYP010000140 DASTYP010000000
VERSION     DASTYP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630238"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFV59_06375"
     CDS             <1..250
                     /locus_tag="VFV59_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325491.1"
                     /translation="ATDATAVALAAIPGVVSVEPTDRAADSRSYQLRIDPDQSAGVQR
                     AVTRLAADNDLTVIDNSLVREGLEDVFMRLVENKERAA"
     gene            247..1026
                     /locus_tag="VFV59_06380"
     CDS             247..1026
                     /locus_tag="VFV59_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5325492.1"
                     /translation="MTAAIFTNSTGFLPGFGALVRRELTEWRRARRTWVVFLVSTVFL
                     TLGSLNSWLISHLPADVTEGAEAPNLDPLANFLGPITTHVFVITAIFAMIAIIAAERE
                     SGTLAWTASKPVSRSAIWMSKFASASGVLWVVAGVLPLAAAVAVVVVLYGAVPVTTIA
                     AVALGMAMIIVLYTAVALAASTVVTSQAAVAGITLVVISVAPMIAQLLPDPTMMPTSI
                     LDWSVRLGVGQATSIVSVIGWGVTVAALVAFSLRRMERLEL"
     gene            complement(1104..3140)
                     /locus_tag="VFV59_06385"
     CDS             complement(1104..3140)
                     /locus_tag="VFV59_06385"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEU5325493.1"
                     /translation="MALILEIAAHGLTAILATWLGLLVYTRARRSTGAGAFAFLALLL
                     ATWSVAIIGQRLGTRPEMHGFLNVLEDFGAFLLPAATAHLAIAIALEGRSNRWATAIL
                     VAGYAVGLVAWVQTALDPTHPILPSPPHWEPLGIPGEIIGWAFILARAGIFAAGIGWL
                     IAALRNARSDVARQRQLHATLVTLGLGIAGGMARILPEGVAGPPWVGVSMVAAAAAIA
                     VYGVFAQRLFTDAELTGRAVRLSLGIGLGVAAYVAALIVLDRAVAAAIGLDLPVVIGL
                     ALVATIALFDPVADFVRALALGRRDEDRTLGRLLRALGEDVVTTRKHDDMVAPAMERL
                     VRTFDLSGAALVDRDGTTVVTTGDVPIDATATRISLDAAGATATFGPKRSGLPLTPAE
                     VDLLRLATGYVDAAIRLGAQQGAQASVLAELSREGEAVDRREAALAEALAAHAATERG
                     LRVYALGPLRAELDGAPLRQWGGAKAGSRQAEAVFAFLFDRGERGAAKEEIVELVWPD
                     IDLERADMAFHRTLLGLRGALAPARAGRSKDGPIVFGNDRYRLAPDVVAWSDLAEFES
                     LVDRARGIADPRERLHLLEAARALYRGDLLDDCPFYGDSAEVEEHREELRERYVDLLV
                     DLGLGYVERGDRSGATSALRRAAALSGRERPVIAEALAGLDVGAAGPSPQPVVG"
     gene            3270..4835
                     /locus_tag="VFV59_06390"
     CDS             3270..4835
                     /locus_tag="VFV59_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEU5325494.1"
                     /translation="MKQDAAISPATDAPASLAATLGTAAIRATLPLVSIGLKLAALDY
                     GTLRWVLETTPPAVTDRLSRERARETARHAARNVPAYRDFLLDQGVDVDRIDRVDVLP
                     ETDKATYVDRWSLEDRCVGGRIPLRGTTIDESSGSTGTPYNWVRSREERLHVRRMIGF
                     FARYTFGDSPLVVLNAFSMGAWATGMTTAIGLESNGLVKATGPDIGKIIGTMRQLGTR
                     YRYLIVGYPPFLKLLLDEGEAIGFDWPAYQMHALLGGEGNSEALRDYLLRRFSSVFSG
                     YGATDIEIGLAAETPVSMALRRLAAERPEISEAMFGETGRSPMVFQYNPLLHHVETNA
                     RGELLFTVTRHATLSPKVRYNVHDEGGVIRDDVLRGRLAAFGLRLRDLVPQAHVRLVR
                     MPYLYVFGRKDSTVSVMGANIYPGDIEAGIFADPALAESVLSFRLGLREERPGETRPI
                     VSVQLARGAPTAELSAALASAIEGQLAGQNRDYQEAMKEYPALMVPIVELYERGTGPF
                     SDDAGRIKHRYLQ"
     gene            4975..6150
                     /locus_tag="VFV59_06395"
     CDS             4975..6150
                     /locus_tag="VFV59_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325495.1"
                     /translation="MAPSRTASERPSQSPAASSPQPVATARPSASVDPPADAGAWVRA
                     DSFEADPGSTYVVDVAAWDRGFVAVGSSMPDKNVMAGPEMPAIWTSSDGTAWESQPVD
                     LRVSNVSLIGIAPVDDGAAVLAIATTSDAYVEGSQPGAMRSYSWISDDGVAWDPVDFG
                     LPADTVVTSFDHGPQGYALAANGGVWYSTDGRNWEETLEDEVVNVVAGEEGFVAIPVR
                     EAQAERAAVASADGRSWFDADPVDGTVVSVAPINGDWLAVVQTDDLGFTIWHSANGLE
                     WNPVLGVDDLTGPDGPKTGRGLEYDSISGVVLAGAAGAGEAFITLTGNHCCAQLGWNL
                     GVYSTTDGRTWSPVASARHDDAGGALVASIAVGADGTAVLGGHIGRGDDALFWIGQR"
BASE COUNT          937 a         2382 c         2277 g          930 t
ORIGIN      
        1 cgcgaccgac gccaccgccg tcgcgctcgc ggccattccc ggcgtggtct cggtcgagcc
       61 gaccgatcgc gccgccgact cgcggagcta ccagctccgc atcgatcccg accagagcgc
      121 cggcgtgcag cgcgccgtca cgcggctggc cgccgacaac gacttgaccg tcatcgacaa
      181 cagcctcgtc cgcgagggcc tggaggacgt cttcatgcga ctcgtggaga acaaggagcg
      241 tgcagcatga ccgccgcgat cttcaccaac tcaaccggct tccttcccgg cttcggcgcg
      301 ctcgtgcgcc gcgagctgac cgagtggcgc cgcgcccggc gcacctgggt ggtgttcctg
      361 gtgagcaccg tcttcctgac cctcgggtcc ctgaacagct ggctcatcag ccacctgccc
      421 gcggacgtga ccgagggtgc cgaggccccg aacctcgacc cgttggccaa cttcctggga
      481 ccgatcacca cgcacgtctt cgtcatcacc gcgatcttcg cgatgatcgc catcatcgcg
      541 gcggagcggg agagtgggac gctggcctgg acggcctcca agcccgtctc gcgcagcgcg
      601 atctggatgt cgaagttcgc ctcggcgagc ggcgtgctgt gggtggtggc gggagtcctg
      661 ccgctggccg ccgccgtggc ggtcgtggtc gtcctctacg gcgcggtgcc ggtgaccacg
      721 atcgccgcgg tcgccctggg catggcgatg atcatcgtgc tctacaccgc cgtcgcgctc
      781 gcggcgtcga ccgtcgtgac gagccaggcg gccgtcgcgg ggatcaccct ggtcgtcata
      841 tcggtcgcac cgatgatcgc gcagctgctg cccgacccga ccatgatgcc gacctccatc
      901 ctcgactggt ccgtccggct gggcgtgggc caggcaacga gcatcgtgtc ggtcatcggg
      961 tggggcgtca cggtcgcggc gctggtcgcg ttctcgctcc ggcggatgga gcgcctcgag
     1021 ctgtagcggt cggatccgcc ccaggcacga tcggcctccc ggcttccggg gggccgatcg
     1081 gtatgccgcg gcgaccgcgc gccctatccg accaccggct ggggactcgg ccccgcagca
     1141 ccaacgtcga gtccggcgag ggcctccgcg atgacgggtc gctcgcgccc gctgagcgcc
     1201 gccgcgcgac gcagcgcgct ggtcgcaccg gagcggtcgc cacgctccac gtagcccagg
     1261 ccgaggtcga cgagcaggtc gacgtaccgc tcccgaagct cctcgcggtg ctcctcgacc
     1321 tccgcgctgt caccgtagaa cgggcagtcg tcgagcaggt cgccgcggta cagcgcacgc
     1381 gcggcctcca ggaggtggag gcgttcgcgc ggatcggcga tccctcgtgc tcggtccact
     1441 agagactcga actcggcgag gtcgctccat gccacgacgt ccggtgccag ccgatatcgg
     1501 tcattgccga agacgatcgg accatccttc gagcgtccgg cgcgcgccgg ggcgagcgcc
     1561 ccgcgcaggc ccaggagcgt gcggtggaac gccatgtccg cgcgctcgag gtcgatgtcc
     1621 ggccagacga gctcgacgat ctcctccttc gcggcaccgc gctcgccgcg gtcgaacagg
     1681 aaggcaaaga ccgcttcggc ctgccgcgag ccggccttcg cgccgcccca ctgccggagc
     1741 ggcgcgccgt cgagctccgc ccgtagcggc ccgagcgcgt acacccgcag acctcgctcg
     1801 gttgcggcgt gcgccgcgag cgcctccgcc agcgccgcct cgcgacggtc gaccgcttcg
     1861 ccctcgcgcg acagctcggc gagcaccgat gcctgcgcgc cctgctgcgc tccgagccga
     1921 atcgcggcgt cgacgtagcc ggtcgccagg cgcaggaggt ccacctccgc gggcgtcagc
     1981 ggcaggcccg atcgcttcgg accaaacgtc gccgtggccc cggccgcgtc gagcgagatc
     2041 cgcgtggccg tcgcgtcgat cgggacgtcg ccggtcgtga ccaccgtggt gccgtcgcgg
     2101 tcgaccaggg cggccccgga aagatcgaag gttcgcacca ggcgctccat ggccggcgcg
     2161 accatgtcgt cgtgcttgcg tgtcgtcacg acgtcctcgc ccagcgcgcg cagcagccgg
     2221 cccagggttc ggtcctcgtc ccgccgtccc agtgccagcg cacgaacgaa gtcggcgacc
     2281 gggtcgaaca gcgcgatggt ggccaccagc gccaggccga tcacgaccgg caggtccagg
     2341 ccgatggccg cggccaccgc ccggtccagc acgatgagcg cggcgacgta ggccgccacc
     2401 ccgagcccga tcccgagcga gaggcggacc gcgcgtccgg tgagctccgc atcggtgaag
     2461 aggcgctgcg cgaagacgcc gtacaccgcg atggccgcgg ccgccgccac catcgaaacg
     2521 cccacccacg gcggacctgc cacgccttcg gggaggatcc gggccatgcc gccggcgatc
     2581 ccgaggccga gcgtcacgag cgtcgcgtgc agctgccgct ggcgcgccac gtccgagcgg
     2641 gcgttgcgca gggccgcgat cagccacccg atccccgcgg cgaagatgcc ggcccgcgcc
     2701 aggatgaacg cccagccgat gatctcgccc ggaatgccga gcggctccca gtgcggtggg
     2761 ctcgggagga tcggatgggt cggatccagc gccgtctgca cccaggcgac gagcccgacg
     2821 gcgtatcccg ccaccaggat cgccgtcgcc cagcggttgc tccgaccctc aagcgcgatg
     2881 gcgatggcca ggtgcgccgt ggccgcgggg agcaggaacg ccccgaagtc ctccaggacg
     2941 ttgaggaagc cgtgcatctc cgggcgcgtg ccgagccgct ggccgatgat cgccaccgac
     3001 cacgtggcga ggagcagggc gaggaaggcg aacgctccgg cgccggtcga acggcgggcc
     3061 ctggtgtaga cgaggaggcc gagccacgtc gcgagaatgg ccgtcaggcc gtgcgccgcg
     3121 atctccagga tgagcgccat gaacgcgccc cccgtgctcg ggtcgggttg ggcagcggcg
     3181 ccaccctacc ccagccgagc gcgaagcggc aatatgccgt tcggcaccgc agtcgctccg
     3241 caggctgcgc gccgtagggt ggagaggcca tgaagcagga cgccgcgatc agccccgcga
     3301 ccgatgcgcc ggcgagcctc gccgccaccc tgggcacggc cgccatccgc gccacgctcc
     3361 cgctggtcag catcggcctg aagctggctg ccctcgacta cgggacgctc cggtgggtgc
     3421 tcgagacgac gccgccggcc gtcaccgacc ggctctcgcg ggagcgcgcg cgcgagacgg
     3481 cacgccacgc ggcgcggaac gttcccgcgt accgcgactt cctgttggac cagggcgtcg
     3541 acgtcgaccg catcgaccgc gtggacgtcc tccccgaaac cgacaaggcg acgtacgtcg
     3601 accgctggtc gctcgaggac cgatgcgtcg gcggtcgcat cccgctgcgc ggcacgacca
     3661 tcgacgagag ctccggcagc accggcacgc cgtacaactg ggtgcgatcg cgagaggagc
     3721 gcctccacgt gcgccggatg atcggcttct tcgcgcgcta cacgttcggc gactcgcccc
     3781 tggtcgtgct gaacgccttc agcatgggcg cctgggccac cgggatgacg acggccatcg
     3841 ggctggagtc gaacgggctg gtcaaggcaa ccggaccgga catcggcaag atcatcggca
     3901 ccatgcgcca gctcggcacg cggtaccgct atctgatcgt tggctacccg ccgttcctga
     3961 agctgctcct cgacgagggt gaggccatcg gcttcgactg gccggcgtac cagatgcacg
     4021 ccctgctcgg cggggagggg aactcggagg cgctgcgcga ctacctgctg cggcggttca
     4081 gctcggtgtt ctccggctac ggcgcgaccg acatcgagat cggcctcgcc gcggagacgc
     4141 cggtgtcgat ggcgttgcga cggctggcgg ccgagcgccc ggagatctcc gaggcgatgt
     4201 tcggtgagac cggccgcagc cccatggtct tccagtacaa cccgctgctt caccacgtcg
     4261 agacgaacgc gcgcggcgag ctgctcttca ccgtcacgcg ccatgcgacc ctgtcgccca
     4321 aggttcgcta caacgtccac gacgagggcg gcgtcatccg cgatgacgtg ctgcgcgggc
     4381 gcctcgccgc gttcggcctt cgcctgcgcg acctggtgcc gcaggcccac gtgcgcctgg
     4441 tgcggatgcc gtacctgtac gtcttcgggc ggaaggactc gaccgtatcg gtaatggggg
     4501 cgaacatcta tcccggcgac atcgaggcgg gcatcttcgc cgacccggcc ctggcggagt
     4561 cggtgctgtc cttccggctg ggtctgcggg aggagcgtcc gggcgaaacg cggccaatcg
     4621 tatcggtgca gctggctcgc ggtgcaccca ccgcggagct cagtgccgcg ctggcatcgg
     4681 cgatcgaggg gcagctggcc ggtcagaatc gtgactacca ggaggcgatg aaggagtacc
     4741 cggcgctgat ggtgcccatc gtcgagctgt acgagcgagg caccgggccg ttctcggacg
     4801 acgccggccg gatcaagcat cgctacctgc agtaggggag gaagaccagc cgtgcaaccc
     4861 acccggcctc gcgcgagccg cctgaacacc ctgtccggcc tggccgtctc cgccgtgctc
     4921 gcggcctgcg gaaccaccgg gatcgatgcg tcctcgggcc cctcgagctc ggcggtggca
     4981 ccatcccgca ccgcgtccga acgtccgagc cagtcgccag cagcctcgtc cccgcagccg
     5041 gtcgccaccg cccgtccgtc agccagcgtc gatccgcctg cggatgccgg cgcctgggtc
     5101 cgcgccgaca gcttcgaggc cgatccgggc tcgacctacg tcgtcgatgt cgcggcgtgg
     5161 gaccgcgggt tcgtcgccgt gggctcctcg atgcccgaca agaacgtcat ggccggaccc
     5221 gagatgccgg cgatatggac ctcgtcggat ggcaccgcgt gggagtcgca gcccgtcgat
     5281 ctccgcgtct caaacgtctc gctgatcggg atcgcgcccg tcgatgacgg cgccgccgtc
     5341 ctggccatcg cgaccacctc agacgcctac gtcgaagggt cccagccggg tgcgatgcgc
     5401 tcgtactcgt ggatctcgga cgacggcgtc gcctgggatc ccgtcgattt cggtctgccg
     5461 gcggacaccg tcgtcacctc gttcgaccac gggccgcagg gctacgcgct cgcggcgaac
     5521 ggtggcgtct ggtactcgac ggacggccgc aactgggaag agacgcttga ggacgaggtc
     5581 gtcaacgtcg tggcggggga ggagggcttc gtcgccatcc cggtgaggga agcacaggcg
     5641 gagcgcgcgg ccgtcgcctc cgccgatggc cggtcgtggt tcgacgccga cccggtcgac
     5701 ggcaccgtcg tgtccgtcgc gccgatcaac ggcgattggc tcgccgtggt tcaaaccgat
     5761 gacctgggct tcacgatctg gcactctgcg aacggcctcg agtggaaccc ggtcctcggc
     5821 gtggacgatc tgactgggcc tgacgggccg aagacaggcc gtggcctcga gtacgattcg
     5881 atcagtgggg tggtgctcgc gggtgcggcg ggtgccgggg aggcgttcat cacgctgacc
     5941 ggcaaccatt gctgtgcgca gcttggctgg aacctcggcg tctactcgac gacggacggc
     6001 cgcacctggt cacctgttgc ctcggcgaga cacgacgacg cgggtggcgc gctcgtggcc
     6061 agcatcgccg tcggagcgga cggcaccgcg gtgctcggtg ggcatatcgg ccgcggcgac
     6121 gacgcgctct tctggatcgg tcagcggtga gattccccgg cggcgtgccg ccgggcgggc
     6181 gtatcgggca cttcctcccc ctttgtgcca ccgggcgggc cgatcgggca agctgacggc
     6241 caaattgcgc tccaggctcc ccatctgtgc cgctccacga gaagaacggg gacttcttcc
     6301 ccgataatcc cgccccacgc aaacggcgtc gggccaacgt cctcccacac ctcagcagtc
     6361 gaagacgaac cagcaaagcc cgtttcgccg gcgctggtcg tccagcacca gctcgcggac
     6421 gcgcgccggc agctgctcgc gctgccactg gcactcgacc cggcccgctc ggtccgcttc
     6481 gtcgcgaggc gccgccgcac ggaccgcctt gatggcgtag gccgcc
//
LOCUS       DASTYP010000141         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1613357, whole genome shotgun sequence.
ACCESSION   DASTYP010000141 DASTYP010000000
VERSION     DASTYP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613357"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..102)
                     /locus_tag="VFV59_06400"
     tRNA            complement(28..102)
                     /locus_tag="VFV59_06400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68..70),aa:Met,seq:cat)
     gene            complement(157..1383)
                     /locus_tag="VFV59_06405"
     CDS             complement(157..1383)
                     /locus_tag="VFV59_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5325496.1"
                     /translation="MRVLLIGVGTVGEAIARLSADRDWCEAMVLADYDESRARTLSDA
                     LGGAPRYPAAKIDARDASAIAELARAHRVDLVMNAVDPQLVMPIFRGALEAGTHYLDM
                     AMSLSKPHPDRPFEQPGLKLGDEQFAMAAEWENRGKLALVGMGMDPGLTDVFAAHAAK
                     HAFDEVHEVHVRDGGDLEIPGYAFAPVFSIWTTIEECLNPPIVWQDGAWHTTEPFSAP
                     EEFPFPEGIGPVECVNVEHEEVLLVPRWIETRRATFKYALGSDFIDKLKTIHALGMDR
                     TDRVRVKGVEVAPRDVLAAITPDPITLGDKFRGRAVVGTWVIGLKDGKPRETFAYQMA
                     DAEDIWRRYGLQVVGWQTGFNPVIAMELLATGAWTGAGVLGPEAFDPDPYLALLDRYG
                     IHHAVVEMQPGAHRPS"
     gene            complement(1477..2151)
                     /locus_tag="VFV59_06410"
     CDS             complement(1477..2151)
                     /locus_tag="VFV59_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5325497.1"
                     /translation="MAGQTLDTTKTPTPTSTTPTDGQLLEAIARGDESSLAALYDRYH
                     SLAFGLALRVLNDRGRAEDVVQDAFLAVWRKAGSYAEARGSVKTWLATIARNRAIDIV
                     RGRRESDSDDEAVLLALEDSRPSVLEQVTASLDRDVLRTVVASLPAEQRQAIAMAYFE
                     GLSHSEIAAATDLPLGTVKSRIRLGMQRLKAQLLEAGMQPDHEPVSAIAGIGAIIRTA
                     PPSAVL"
     gene            2220..3110
                     /locus_tag="VFV59_06415"
     CDS             2220..3110
                     /locus_tag="VFV59_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5325498.1"
                     /translation="MYTIKQAAARSGVNIALLRAWERRYHVVEPTRTDAGYRLYDDDA
                     IARLRAMRSLVEGGWSPRQAAAHLEEASPDELRALSTVEATAPATAVPENVVTAFVDG
                     AARLDAAAVERVLDEMFASGTFERVMEDHLFPALRALGDAWAAGQVDVAGEHAASGAV
                     LRRLSMAFEAAGSGSSAESPIVVGLPPGARHEIGPLAFATSARRAGIPVVYLGADVPV
                     ASWVSAASTAGARAVAFGTLLEADVPAANEVVAALRAAVPDVVVAVGGRRSAEVGNGG
                     VVRLSPRIGDAVAELRAALG"
     gene            3144..3476
                     /locus_tag="VFV59_06420"
     CDS             3144..3476
                     /locus_tag="VFV59_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU5325499.1"
                     /translation="MILVIGVLVGLFVVPDPWTVPVIAAAAVLEVVETFVSFRIAKRF
                     GRPRVGPERLPGATGRVVTTCRPRGQVRVRGEVWQARCEAGADVDDPVRVIRRHGLVL
                     DVEPISEP"
BASE COUNT          467 a         1179 c         1269 g          567 t
ORIGIN      
        1 ggaggcggct ttcgtttctc gtctgcgtgg ttgcggggga cggattcgaa ccgccgacct
       61 tcgggttatg agcccgacga gctaccactg ctccaccccg cggcctccaa tgctagagag
      121 tgccgcccgt tcggtcaaac gagggtagga accgcgtcag ctcggccggt gcgcccccgg
      181 ctgcatctcg acgacggcgt ggtggatgcc gtatcggtcc agcagcgcga ggtatgggtc
      241 cggatcgaag gcctcgggcc cgagaacccc ggcgccggtc caggcgccgg tggccagcag
      301 ctccatggcg atgaccgggt tgaagcccgt ctgccagccc acgacctgga gtccgtatcg
      361 gcgccagatg tcctccgcgt ccgccatctg gtacgcaaac gtctcgcgcg gcttgccgtc
      421 cttgaggccg atgacccagg ttccgaccac ggcgcggccg cggaacttgt cgcccagggt
      481 gatcggatcg ggcgtgatgg cggccaggac gtcccgcggg gcaacctcga cgcccttgac
      541 ccggacgcga tcggtccggt ccatgccgag ggcgtggatg gtcttcagtt tgtcgatgaa
      601 gtcggagccg agcgcgtact tgaaggtcgc gcgccgcgtc tcgatccagc gtggcaccag
      661 gaggacctcc tcgtgctcga cgttgacgca ctcgaccggg ccgatgccct ccgggaacgg
      721 gaactcctcc ggcgcgctga atggctcggt ggtatgccag gcgccgtcct gccagacgat
      781 cggcgggttg aggcactcct cgatggtggt ccagatgctg aacaccggcg cgaaggcgta
      841 gccggggatc tcgaggtcgc cgccgtcgcg gacgtggacc tcgtgcacct cgtcgaaggc
      901 gtgcttggcg gcgtgcgcgg cgaagacgtc ggtcaggccg ggatccatgc ccatcccgac
      961 cagcgccagc ttcccgcggt tctcccactc ggccgccatg gcgaactgct cgtcgcccag
     1021 cttcagaccg ggctgctcga agggccgatc cgggtgcggc ttggacaggc tcatggccat
     1081 gtccaggtag tgcgtgccgg cctcgagggc gcctcggaag atcggcatca ccagctgcgg
     1141 gtcgacggcg ttcatgacca ggtcgacccg gtgggctcgg gcaagctcag caatggctga
     1201 tgcgtcccgc gcatcgatct tggcggccgg gtagcgcggg gcgccgccca gcgcatccga
     1261 gagggtgcgg gcgcgagatt cgtcgtagtc ggcgaggacc atggcctcgc accagtcgcg
     1321 gtcggcggag aggcgggcga tggcctcgcc cacggtaccg acgccgatca gcaggactcg
     1381 catggggtca ccagcgtgcg tgttccgata cgggcacgcg tctgcgcaac aggatctcgg
     1441 tgccggcgac ctaccgaatc ggtggaggat tcgctcctac aacaccgcgg acggtggcgc
     1501 ggtccggatg atagcaccga ttccggcgat cgcggacacc ggctcgtggt ccggctgcat
     1561 gcccgcctcg agcagctgcg ccttgagccg ctgcatgccg aggcgaatgc ggctcttcac
     1621 cgttccgagc ggcaggtccg tcgccgcggc gatctccgag tgtgagagcc cctcgaagta
     1681 cgccatggcg atcgcctggc gctgctcggc gggaagcgat gcgacgacgg tccgcaacac
     1741 gtcgcggtcg agcgacgcgg tcacctgctc gagcacgctc ggtcgcgaat cctcgagggc
     1801 caggagcacc gcctcgtcgt cactgtccga ctcgcggcgt ccgcggacga tgtcgatcgc
     1861 ccggttgcgc gcgatggtcg ccagccaggt cttgacgctg ccgcgggcct cggcatacga
     1921 gccggccttg cgccagaccg ccaggaaggc gtcctgaacg acgtcctcgg cacggccgcg
     1981 gtcgttgagc acgcggaggg caaggccgaa ggccagggag tggtatcggt cgtagagagc
     2041 ggccaggctg gattcgtcgc cccgcgcgat cgcctccagg agctgaccgt ccgtcggtgt
     2101 cgtcgatgtg ggtgtcggtg tcttggtcgt gtccaaggtt tgtcctgcca tgcgccggac
     2161 tgtagagcct tcacacgggg cttgtcaagc gtttgttcgc gagtatcatt ggacgaagca
     2221 tgtacacgat caagcaggca gccgcccggt ccggtgtcaa catcgcgctc ctccgcgcct
     2281 gggagcgtcg ctaccacgtc gtggagccga ccaggaccga tgcgggctac cgcctctacg
     2341 acgacgacgc catcgcccgc ctgcgcgcga tgcggtcgct ggtggaaggt gggtggtcgc
     2401 ctcggcaggc ggcggcccac ctggaggagg catcgccgga tgagctgcgg gcgttgtcga
     2461 cggtcgaggc cacggctccg gcgacggccg tgcccgagaa cgtcgtcacc gcgttcgtgg
     2521 acggcgccgc ccggttggac gccgctgcgg tcgagcgcgt gctcgacgag atgttcgcgt
     2581 ccggcacctt cgagcgcgtc atggaggacc acctcttccc ggcgctccgg gcgctcggcg
     2641 atgcgtgggc cgccggccag gtcgacgtgg cgggcgagca tgccgccagc ggcgcggtcc
     2701 tgcggcggct cagcatggcg ttcgaggcgg ccggcagcgg ttcgtccgcc gagtccccga
     2761 tcgtcgtggg cctgccgccc ggcgcgcgcc acgagatcgg cccgctggcc ttcgccacgt
     2821 ccgcccgacg cgcgggtatc cctgtcgtct acctgggcgc cgatgtcccc gtcgcatcct
     2881 gggtcagcgc ggcgtcaacc gccggggcgc gggcggtcgc cttcgggacg ctgctggagg
     2941 ccgacgtccc tgccgccaac gaggtcgtcg ccgccttgcg agccgccgtg ccggacgtcg
     3001 tggttgcggt cggcggtcgt cggtcagctg aggtcgggaa cggcggcgtg gtgcggctca
     3061 gtccacggat cggtgatgcc gtcgctgagc tgcgggccgc cctcggctga cgcctgggcc
     3121 tggcccgagt agcctcgtcg cccatgatcc tcgtcatcgg cgtcctggtc ggcctcttcg
     3181 tggtccccga cccgtggacc gtgccggtca tcgccgccgc cgcggtcctg gaggtcgttg
     3241 agacgttcgt gtcgttccgg atcgcgaagc gcttcggacg gccgagggtc ggtcccgaac
     3301 gactgccggg cgccaccgga cgcgtcgtca cgacctgccg gccgaggggc caggtccgcg
     3361 tgcgcggtga ggtgtggcag gcacgttgtg aggccggcgc cgacgtggac gatccggttc
     3421 gcgtgatccg gcgacacgga ctggtgctgg acgtcgaacc gatctccgag ccctgaccga
     3481 tg
//
LOCUS       DASTYP010000142         9065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1103568, whole genome shotgun sequence.
ACCESSION   DASTYP010000142 DASTYP010000000
VERSION     DASTYP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103568"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VFV59_06425"
     CDS             complement(<1..1589)
                     /locus_tag="VFV59_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5325500.1"
                     /translation="MTWDRVAVVERQLDTYLASAESAPRRLAPHEPITPDGSLTAAVG
                     RALFEDQLVSRQLDVAARELKKTDRSFYTISSAGHENNAVVGARLRVDDPAFLHYRSG
                     GFVMARARQLAGSTPAFDTLLGVVASSDDPIAQGRHKVWGSRAMWIPPQTSTIASHLP
                     KAMGLAFSLARAKRLGVASGLPADAVVACSFGDASANHATALAGINTARYAVRMGLPM
                     PILFVCEDNDTGISVPTPAGWIAESFGNLPHLRYVLADGEVDEVWDAVGEAVQHVRTT
                     RQPAFLHLRTVRLWGHAGSDAEHGYRALAEIAEVEARDPLLRNARRLIETGAATPEEL
                     AELVRDTRDRIAALAEEAARRPRLQTTDEVVAPMAPSNPERVLTNATSLVVDEDTRRR
                     SFGGTLPEEATLPQARTLAGCINAALADELLRRPELLLFGEDVGRKGGVYHLTSGLQK
                     RFGPARVFDTLLDETSILGIAQGAAHIGLLPVPEIQYLAYIHNALDQLRGEAASLQFF
                     SSGQFRNPMVVRVAGLAYQKG"
     gene            complement(1650..1775)
                     /locus_tag="VFV59_06430"
     CDS             complement(1650..1775)
                     /locus_tag="VFV59_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325501.1"
                     /translation="MLSRRLRHPFIWSPVAATLLQLVLAAAVAASTGGGDFPLRR"
     gene            complement(1924..2757)
                     /locus_tag="VFV59_06435"
     CDS             complement(1924..2757)
                     /locus_tag="VFV59_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5325502.1"
                     /translation="MPIAGVAAGLLAALSFGAGDFAGAVAARRAGALAVVAGAHVSGL
                     VVLVTAALLLAPPMPGGPAILTALAAGVAGGVGLAALYRGMALGSMGLITALSGAGSL
                     AIPLLFGAILGDRVGPLQIAGVGCAATAAIAASGASRSELGRVAIALAGLAAVSFGTW
                     YVLIDLAAERGDPLWALVFSRAASAGIAAVLAIGRVDRSRYPLRVVVAAGLGDIGGNA
                     LYVVARDQMPVGLAAALTGLYPIVTMLLARFVLGEHLPRLGQVGVALALLGILLISLG
                     G"
     gene            2968..4137
                     /locus_tag="VFV59_06440"
     CDS             2968..4137
                     /locus_tag="VFV59_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020517454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325503.1"
                     /translation="MGAPARAAATAVGSEVQVAVETRDHVQPTSRATSDLPALRLEGV
                     VKRFGDVVAVNGIDLEVADGEFFSMLGPSGSGKTSTLRMIAGFEEPTEGRILLHGRDV
                     TGVPPFDRDVNTVFQDYALFPHMTVGDNVGYGLLVRKVPTAERRQRVTDALRMVRLDG
                     YEKRRPSQLSGGQRQRVALARALVNRPRVLLLDEPLGALDLKLREEMQIELKAIQQQV
                     GITFVYVTHDQEEALTMSDRLAVFNLGRIEQIGTPAEVYERPASTFVAGFVGTSNLLT
                     GETARAVIGREGIFTVRPEKIRLADPSERPADDEESALGRIREVVYVGPDTRYIVELE
                     VGGELVVTQQNLETSSMEALETQGRPVRLVWKRRHVLSLARSEERPGDTEENTSR"
     gene            4134..5306
                     /locus_tag="VFV59_06445"
     CDS             4134..5306
                     /locus_tag="VFV59_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5325504.1"
                     /translation="MRHLRVPGLLTVVAMLLAACGGAGGSAGPTPINEIGDGEGELNL
                     VIWAGYAERGETVAEYDWVTPFEEETGCIVNTTNMTDSNNGVSLIQSGEYDGISASGD
                     ATSRLIASDLVAPVNTDVFENYDAVFDDLKDLPHNTVDGVNYGVPHGRGPNLLAWNTE
                     EVTDAPTSWSPIWEDATDHAGKISIYDSSIFIADAALHLMSTQPDLGIEDPYQLNQEQ
                     FDAAVALLQELDANDPLYWSDFNDQIASYASGDVVVGTTWPYQVNLLQAEGEPIEATL
                     PEEGATGWSDTWMMYAEAEHPNCMLMWMDHMMSPEANAQATVWFGEAATSEAACEAAE
                     ALSEGHCEAQHANDAAYWEKVWYWSTPQEDCADDDSATTCVTQEDWVDAWTSIRGS"
     gene            5373..6284
                     /locus_tag="VFV59_06450"
     CDS             5373..6284
                     /locus_tag="VFV59_06450"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325505.1"
                     /translation="MTAPSSFRDLAAPVPGRLRRFASWLHRHPRTQLAMLLSGPLGWM
                     VIAYLGSLFILLLGAFWDRDPFTGQVEPFTWSLDAFEDLVTNPVYRTIAIRTITIAAA
                     VTITDALLAFPIAYYMARVASPRVRQLLVVAVLLPLWAAYLVKVYAWRTILQGNGFLE
                     WALTPFGVDAPGLGQILNAWVVLSYLWLPYMILPIFAGLERIPNSLHEASADLGARSG
                     ATFRRVTLPLVFPSLVAGSIFTFSLTLGDYITPDLVADAKFVGNVIYDNSSLGNLPLA
                     AAYSLLPIVIMIWYLLVARRLGAFESL"
     gene            6295..7095
                     /locus_tag="VFV59_06455"
     CDS             6295..7095
                     /locus_tag="VFV59_06455"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325506.1"
                     /translation="MTESFWTRVGLRAATVVVLLFLYLPLLILAIYAFNPSRLQVWPP
                     TGLTLHWFAEAAGNPGLRAAVLNSLIAGAGATSIALVLGTAAALAVQRYSFFGRDVIS
                     FLFVLPIALPGVVTGIALRTTFQTLDVDFGLGTIIVGHATFCVVIAYNNVIARLRRTS
                     RSPEEASADLGADTWTTFRRITVPAMGTALLSGGLLAFALSFDEIIVTNFTAGPGTQT
                     IPMWILAAIQRPAEVPVVNVVAFVLILLSVIPVYLAERVSAGVGGGRY"
     gene            7210..8946
                     /locus_tag="VFV59_06460"
     CDS             7210..8946
                     /locus_tag="VFV59_06460"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEU5325507.1"
                     /translation="MTTELAPPARTMPRSLPIIAAVAVAAITVGGVAGAFLLGDRTAG
                     AGSAAGYVPADVPFYLELRLDPSDAQDAALRELLGHFPPIEGIDLDRPLFDQLGEAVD
                     EAAASADETDLRWADDVATWFDGHVTLAVTDVPLEPAADGSEPMPEMLVVLGSTDADA
                     ARAATERMRAEAGTDVQFTETEHRGVTITTTADEQGAYAITDDAVLLAPTAAEIEAAL
                     DIQAGAAESLGDGERLADAAGELPDDWLAFAVYDVGDLVSASLDAGDAPDVSTEPLRA
                     LLAQQPMRGAMAVTADGDRLALTSVSDVPTGDFTMENADRGLADEVPADALYYADGGN
                     IGSSLAAFITALKESVAGQPEAADQIATAEAALGADLEELVSWVDDAALVIGMDGDTP
                     YGGAVLVPSDADAPQRRIGQLVTFARLGAMDSSSGITVDERDVDGTTVTSITWRPASF
                     GADGMMAGPDGATIEVATTDDRAYLAVGEGSVERLLTVDAGDSLATVDRFTDAVDQLG
                     GSDNVGTVWLDLRGVRTAIESALGPMLDEMDPDGAYQREVLPWLEPLDRMVGVTRVEG
                     DRAVQRSVLLVD"
BASE COUNT         1321 a         3253 c         3241 g         1250 t
ORIGIN      
        1 aagcccttct ggtacgccag gccggcgacc cgcacgacca tcgggttgcg gaactggccg
       61 gatgagaaga actgcagcga agcggcctcg ccccgcagct ggtcgagcgc gttgtggatg
      121 taggccaggt actggatctc cggcacgggg agcaggccga tgtgcgccgc gccctgcgcg
      181 atcccgagga tgctggtctc gtccagcagg gtgtcgaaca cgcgcgccgg gccgaaccgc
      241 ttctggaggc cgctggtcag gtggtagacg ccacccttgc ggccgacgtc ctcaccgaac
      301 aggagcagct cggggcgtcg cagcagctcg tcggcgagcg ccgcgttgat gcagccggcc
      361 agcgtgcggg cctgcggcag tgtcgcctcc tccgggagcg tgccaccgaa tgaccggcgg
      421 cgcgtgtcct cgtccacgac cagcgacgtg gcattggtca gcacccgctc ggggttcgac
      481 ggcgccatcg gcgcgacgac ctcgtcggtc gtctggagcc gcgggcgacg cgccgcctcc
      541 tcggccagcg cggcgatccg atcgcgggtg tcgcggacca gctcggccag ctcctcgggg
      601 gtggccgctc cggtctcgat caggcgacgt gcgttccgca ggagggggtc gcgcgcctcc
      661 acctcggcga tctcggcgag ggcgcggtag ccatgctccg cgtcgctgcc ggcatgcccc
      721 cacagccgga ccgtgcggag gtgcaggaag gctggctggc gggtggtgcg gacgtgctgc
      781 accgcctcgc cgaccgcgtc ccacacctcg tcgacctccc cgtccgcgag tacgtagcgc
      841 aggtgcggca ggttgccgaa cgactcggcg atccaccccg ccggcgtggg gacgctgatg
      901 ccggtgtcgt tgtcctcgca gacgaagagg atcggcatgg gcaggcccat gcgcaccgcg
      961 tagcgcgccg tgttgatccc ggccagggcc gttgcgtggt tggcgctggc gtcgccgaag
     1021 ctgcacgcca ccacggcgtc ggccggcagt ccactggcga cgccgagccg cttcgcgcgg
     1081 gcgaggctga aggccagccc catggccttc ggcaggtggc tggcgatcgt cgaggtctgc
     1141 ggcgggatcc acatggcgcg gctgccccat accttgtggc ggccctgggc gatgggatcg
     1201 tcactggacg ccaccacgcc gagcagcgtg tcgaacgccg gcgtgctgcc ggccagctgc
     1261 cgcgcgcgag ccatgacgaa cccgcccgag cggtagtgca ggaaggccgg atcgtcgacg
     1321 cgcaggcgag cgccgacgac cgcgttgttc tcgtgcccgg ccgagctgat ggtgtagaag
     1381 ctgcggtccg tcttcttcag ctcccgggcc gcgacgtcca gctgccgcga gacgagctgg
     1441 tcctcgaaca gcgctcggcc gaccgccgcc gtcagcgagc cgtccggcgt gatcggctcg
     1501 tgcggcgcga ggcgccgggg tgccgattcg gcggaggcga ggtaggtgtc cagctggcgt
     1561 tcgacgacgg cgacgcggtc ccaggtcatg cgctgctcat ggctcggagg cgatcgaggg
     1621 cgggccgcca tgatagccgc ccccggcggt taccgacgca gcgggaagtc gccaccgccg
     1681 gtcgacgcgg cgaccgccgc ggcaaggacc agctgcagca gcgttgcggc aacgggcgac
     1741 cagatgaagg ggtggcgcag gcggcgggac agcatgggag gggacctcga tccgggcgcc
     1801 gcggcggccc tctgccgccg cacgagcgcc gctcccattc caccgaatcg gcggttaccg
     1861 gcggcttacc agcggcttac tggggctccc cctgccgtgc cacgacgggc gcggccaacg
     1921 ccgtcagccg ccgagcgaga tcagcaggat ccccagcagg gcgagggcga cgccgacctg
     1981 gccgagccga ggcaggtgct cgccgagcac gaagcgcgcg agcagcatcg tgacgatcgg
     2041 gtagagcccg gtgagcgccg ccgccagccc caccggcatc tggtcgcggg ccacgacgta
     2101 cagcgcgttc ccgccgatgt ctcccagccc cgccgccacg acgacgcgca gcgggtagcg
     2161 cgaccgatcg acacgaccga tcgccagcac agcagcgatg ccggccgacg ctgcgcggct
     2221 gaacaccagc gcccagagcg ggtcgccgcg ctcggcggca aggtcgatga ggacgtacca
     2281 cgtcccgaac gacacggcgg cgagcccggc caacgcgatc gccacgcgtc cgagctcgct
     2341 gcgggaggct ccgctggcgg cgatggccgc ggtggcggca cacccgacac cggcgatctg
     2401 cagtgggccg acgcggtcgc cgaggatggc cccgaagagc agcggaatgg cgagcgaccc
     2461 cgccccgctg agggcggtga tgaggcccat gctgcccagg gccatgccgc ggtacagcgc
     2521 cgccaggccg acaccgccgg ccacgcccgc cgccagcgcg gtgagaatcg ccgggccgcc
     2581 gggcatcggt ggcgccagca gcagggccgc ggtgacgagc acgaccaggc ccgagacgtg
     2641 cgcaccggcc acgacggcca gcgcgccggc gcggcgggcg gcgacggcgc cggcgaagtc
     2701 accggcgccg aaggagaggg cagcgaggag tccggcggcg accccggcga tcggcatggt
     2761 gcggcactgt agcggagtgc ggacggcgtc acgtcccgcc atcggcgcgc caccggagcc
     2821 gcctccaacc gcgaaatcct cggaaatcgg gtcgccgcct caccgaattc ggcgaccgcc
     2881 cggaagcggg tcgcggaaac ggcgggatcg gcttgacagg gacactcgcc tcacgcacag
     2941 tgcgcgcacc cgagaccggt ccgtcgaatg ggtgccccgg cccgagccgc ggcgacggcc
     3001 gtcgggagcg aggtgcaggt ggcggtcgag acgcgagacc acgtccagcc gacgtcccgc
     3061 gccacctcgg atctgcccgc gctgcgtctc gagggcgtcg tcaagcggtt cggcgacgtg
     3121 gtcgccgtga acggcatcga cctcgaggtg gccgacggcg agttcttcag catgctcggc
     3181 ccatcgggct cggggaagac gtcgacgctg cgcatgatcg cgggtttcga ggagccgacc
     3241 gagggccgca tcctgctcca cggccgcgac gtcaccggcg tcccgccgtt cgaccgtgac
     3301 gtcaacaccg tcttccagga ctacgccctc ttcccgcaca tgaccgtcgg cgacaacgtc
     3361 gggtatgggc tgctggtgcg caaggtcccg accgccgagc gacgtcagcg ggtgacggac
     3421 gcgctgcgca tggtgcgcct cgacggctac gagaagcggc gacccagcca gctgtcgggc
     3481 ggacagcggc agcgcgtggc gctggctcgc gcactcgtca accggccgcg tgtcctgctg
     3541 ctggacgagc cgctgggggc gctggacctg aagctgcgcg aggagatgca gatcgagctc
     3601 aaggccatcc agcaacaggt cggcatcacc ttcgtgtacg tgacccacga ccaggaggag
     3661 gcgctcacca tgagcgaccg cctggcggtg ttcaacctcg gccggatcga gcagatcggt
     3721 acgccggcgg aggtctacga gcggcccgcg tcgaccttcg tcgcgggctt cgtcggcacc
     3781 tccaacctgc tcaccggcga gaccgcgcgc gccgtcatcg gccgcgaggg gatcttcacc
     3841 gtgcggcccg agaagatccg cctggccgac ccgtccgagc ggcccgccga cgacgaggag
     3901 tccgcgctgg ggcggattcg agaggtcgtc tacgtcgggc ccgacacccg ctacatcgtc
     3961 gagctcgagg tgggaggcga gctcgtcgtg acccagcaga acctggagac ctcgtccatg
     4021 gaggcgctcg agacccaggg tcggccggtc cggctggtct ggaagcgccg ccacgtgctg
     4081 agcctcgcga ggagcgagga acgaccgggg gatacggagg agaacacaag ccgatgaggc
     4141 acctgcgcgt tcccggcctg ctcaccgtcg tcgccatgct gctcgcagcc tgcggcggcg
     4201 ccgggggtag tgccggcccg acgccgatca acgagatcgg cgacggcgag ggggagctca
     4261 acctggtcat ctgggccggc tacgccgagc gcggcgagac cgttgccgag tacgactggg
     4321 tcacgccatt cgaggaggag accggctgca tcgtcaacac gacgaacatg accgactcca
     4381 acaacggcgt ctcgctcatc cagtccggcg agtacgacgg catcagcgcc tcgggcgatg
     4441 ccaccagccg gctcatcgcc agcgacctgg tggccccggt caacaccgac gtcttcgaga
     4501 actacgacgc ggtcttcgac gacctcaagg acctgccgca caacacggtg gacggcgtca
     4561 actacggcgt cccccacggc cgcggcccga acctgctggc gtggaacacc gaggaggtga
     4621 ccgatgcgcc gaccagctgg tcgccgatct gggaggacgc gaccgaccac gccggcaaga
     4681 tcagcatcta cgactcctcg atcttcatcg ccgatgcggc gctgcacctg atgtcgaccc
     4741 aacccgatct cggcatcgag gatccgtacc agctgaacca ggagcagttc gacgcggccg
     4801 tggcgctcct ccaggagctg gacgccaacg acccgctgta ctggagcgac ttcaacgacc
     4861 agatcgcctc gtacgccagc ggcgacgtgg tggtcgggac gacctggccg taccaggtga
     4921 acctgctgca ggccgagggt gagccgatcg aggccacgct gcccgaggag ggcgccaccg
     4981 gctggtcgga cacctggatg atgtacgcgg aggccgagca cccgaactgc atgctgatgt
     5041 ggatggacca catgatgtcg ccggaggcga atgcccaggc gacggtgtgg ttcggcgagg
     5101 cggcgacgtc cgaggcggcg tgcgaggcgg cggaggcgct ctcggagggc cactgcgagg
     5161 cgcagcacgc caacgacgcg gcctactggg aaaaggtctg gtactggagc acgccccagg
     5221 aggactgcgc cgacgacgac tccgccacca cctgcgtgac gcaggaggac tgggtcgacg
     5281 cgtggacctc gatccgcggc tcgtgaccgt cccttcgtga acgaaccgat gctccccggg
     5341 gtccgctccg gggagcatca ttcgtccgtc gcatgaccgc cccctcctcg ttccgtgacc
     5401 tcgccgcacc ggtgcccggt cggctgcggc ggttcgcgtc gtggctgcat cggcacccac
     5461 gcacgcagct ggcgatgctg ctgtccggcc cgctgggctg gatggtcatc gcctacctcg
     5521 ggtcgctgtt catcctgctg ctcggtgcct tctgggatcg cgacccgttc accggccagg
     5581 tggagccgtt cacctggagc ctcgacgcgt tcgaggacct ggtcaccaac ccggtgtatc
     5641 ggacgatcgc catccgcacg atcacgatcg ccgccgcggt caccatcacc gatgcgctgc
     5701 tggccttccc gatcgcctac tacatggcgc gcgtggcatc gccgagggtc cgacagctgc
     5761 tggtggtggc cgtgctcctg ccgctctggg cggcgtacct ggtcaaggtc tacgcctggc
     5821 gcacgatcct gcagggcaac ggcttcctgg aatgggcgct gaccccattc ggcgtcgacg
     5881 cgccgggcct gggccagatc ctgaacgcct gggtcgtgct gagctacctg tggctgccct
     5941 acatgatcct gccgatcttc gccgggctcg agcgcatccc gaactcgctg catgaggcct
     6001 cggccgacct cggcgcgagg tccggcgcca ccttccgacg cgtgacgctg ccactggtct
     6061 tcccgtcgct cgtggccggc tcgatcttca ccttctcgct caccctcggc gactacatca
     6121 cgcccgacct ggtggccgat gcgaagttcg tcggcaacgt catctacgac aactccagcc
     6181 tcgggaacct gccgctggcg gcggcctaca gcctcctgcc catcgtgatc atgatctggt
     6241 acctgctcgt cgcccgacgc ctgggcgcct tcgagtcgct gtgagaccga tgcgatgacc
     6301 gagtcgttct ggacgcgggt gggcctgcgc gcggcgaccg tcgtggtgct gctgttcctc
     6361 tacctgccac tcctgatcct ggcgatctac gccttcaacc cgtcccgcct ccaggtgtgg
     6421 ccgccgaccg gcctgacgct gcactggttc gccgaggcgg ccggcaaccc ggggctgcgg
     6481 gcggccgtgc tcaattcgct gatcgcgggg gccggcgcca ccagcatcgc gctggtgctc
     6541 gggacggcgg ccgccctggc ggtgcagcgc tacagcttct tcggccgcga cgtcatctcc
     6601 ttcctgttcg tgctgccgat cgcgctgccg ggcgtggtca ccggcatcgc gctgcgcacc
     6661 acgttccaga ccctggacgt cgacttcggg ctgggcacga tcatcgtggg ccacgcgacc
     6721 ttctgcgtgg tcatcgcgta caacaatgtc atcgcccgcc tgcggcgcac gtcgcgcagc
     6781 cccgaggagg cgtccgcgga cctcggtgcc gacacgtgga cgaccttccg tcgcatcacc
     6841 gtcccggcca tgggcaccgc gctcctctcg ggcgggctgc tcgccttcgc gctgagcttc
     6901 gacgagatca tcgtcaccaa cttcacggca gggccgggga cccagacgat cccgatgtgg
     6961 atcctggcgg ccatccagcg ccccgccgag gtgccggtgg tgaacgtggt ggccttcgtc
     7021 ctcatcctgc tgtcggtcat cccggtctac ctggccgagc gggtcagcgc gggggtcggc
     7081 ggcggccgct actgagggga ccgggcggcc aaggccgaat ggtccttgcg tccggccggc
     7141 agatcgggcg taccctacgt cctgatgccc ggccgcccga gggccggtac cccaggggag
     7201 agcccatccg tgaccactga actcgctccg ccggcccgca ccatgccccg gagcctcccg
     7261 atcatcgccg ccgtcgccgt ggccgccatc acggtcggcg gcgtggccgg cgccttcctg
     7321 ctcggcgatc ggacggccgg tgccggcagc gccgccggct acgtcccggc cgacgtgccg
     7381 ttctacctcg agctgcggct cgatccctcc gacgcccagg acgccgccct gcgcgagctg
     7441 ctgggccatt tcccgccgat cgagggcatc gacctggacc gcccgctgtt cgaccagctc
     7501 ggcgaggccg tcgacgaggc cgccgccagc gccgacgaga cggacctgcg ctgggcggat
     7561 gacgtcgcga cctggttcga tggtcacgtc acgctcgccg tcacggacgt gccgctcgag
     7621 ccggccgccg atgggtcgga gccgatgccc gagatgctcg tcgtcctcgg ctcgaccgat
     7681 gcggacgccg cccgtgccgc caccgagcgc atgcgcgccg aggccgggac ggacgtgcag
     7741 ttcaccgaga ccgagcaccg gggtgtgacg atcaccacga ccgccgacga gcagggcgcc
     7801 tatgcgatca ccgacgacgc cgtgctgctg gcaccgacgg ccgccgagat cgaggccgcg
     7861 ctggacatcc aggccggcgc cgcggagtcg ctgggcgacg gggaacgcct ggcggacgcg
     7921 gccggcgagc tgccggacga ctggctggcc ttcgcggtgt acgacgtcgg cgacctggtg
     7981 tccgcttcgc tcgacgccgg ggacgcgccg gacgtttcga ccgagccgct ccgggcgctc
     8041 ctggcccagc agccgatgcg cggggccatg gccgtcacgg ccgacggcga ccggctggcc
     8101 ctgacctcgg tcagcgacgt ccccaccggc gacttcacca tggagaacgc cgaccgcggc
     8161 ctcgccgacg aagtcccggc cgatgccctc tactacgccg acggcggcaa catcgggtcg
     8221 tcgctggcgg cgttcatcac ggccttgaag gagagtgtgg ccgggcagcc cgaggccgcc
     8281 gaccagatcg cgacggccga ggctgccctg ggcgccgacc tcgaggagct ggtgagctgg
     8341 gtcgacgacg cggccctggt catcggcatg gacggcgaca ccccgtacgg cggcgcggtg
     8401 ctggtgccca gcgacgcgga tgccccgcag cggcgtatcg gtcagctggt cacgttcgcc
     8461 aggctgggcg ccatggattc gagctccggc atcacggtcg acgagcgcga cgtcgacggc
     8521 accaccgtca cctcgatcac ctggcgtccg gcctccttcg gggcggacgg catgatggcc
     8581 ggccccgacg gggcaaccat cgaggtcgcg acgaccgacg accgggcata cctcgccgtc
     8641 ggcgagggca gcgtggagcg gctgttgacg gtcgacgccg gcgactcgct cgccaccgtg
     8701 gatcgcttca ccgacgcggt cgaccagctg ggaggcagcg acaacgtcgg gacggtgtgg
     8761 ctcgacctgc gcggagtgcg cacggcgatc gagtcagcgc tcggcccgat gctggacgag
     8821 atggaccccg acggcgccta ccaacgcgag gtgctgccgt ggctcgagcc gctggaccgc
     8881 atggtcggcg tgacgcgggt ggagggcgac cgggccgtcc agcgctccgt gctcctggtc
     8941 gactgatgct gccgccgccg ctgcgaccgc tccccgggcc gaacgctccg gggagggtcg
     9001 ccgcggagga gcggctccgg gcggcgggcg tgacgccgta cgcgtggggc aacggaccgg
     9061 gcgac
//
LOCUS       DASTYP010000143         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_739909, whole genome shotgun sequence.
ACCESSION   DASTYP010000143 DASTYP010000000
VERSION     DASTYP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739909"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFV59_06465"
     CDS             complement(<1..296)
                     /locus_tag="VFV59_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5325508.1"
                     /translation="MTHAALRAGERVLDVGTGTGNGAAAAVGEGRRVVGADAAAGMLD
                     IARRRVPDAEFVEADFGELPFEDGAFDVVIAVHAINFARDPVAVLHEWRRVATGA"
     gene            complement(406..1605)
                     /locus_tag="VFV59_06470"
     CDS             complement(406..1605)
                     /locus_tag="VFV59_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5325509.1"
                     /translation="MTTRPPTPRAPADFLNDTVRTMPPSGIRRFFDMLAEMKDVISLT
                     IGEPDFTTPEPITRAAIESLEAGETHYTANGGMLALRELIAANLRDRYGVEYDPRTEL
                     VITVGASEGVDAALRAIVNPGDEVIYHEPCFVAYAPCIQLAGGVAVPVSTTLATDFRL
                     TAEMVEAAVTPRTKAIFLGYPNNPTGAVLDREQLEAIARVADAHDLVVFSDEIYDRLV
                     YGEHRHTAFSSLPRMRDRTVLLGGFSKSYAMTGWRIGYVAAPEGLMTGIAKVHQYGIM
                     CAPTPAQFAGMEALRSGEPHLQAMRAEYDRRRRFMTDRFNEIGLRCFEPMGAFYCFPN
                     VSQATGMTETEFAERLLEEERVGVVPGVAFGRSGAGHVRVCYATAYDQIVEAMNRIER
                     FVERHHA"
     gene            complement(1630..2223)
                     /locus_tag="VFV59_06475"
     CDS             complement(1630..2223)
                     /locus_tag="VFV59_06475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEU5325510.1"
                     /translation="MARSARDPLTVLGVAGSLRAGSYNRGLIRAAAEVAPPGVRVVTH
                     ELDQIPLFNADVEADGDPAAVVAFKDAIGGADALLIATPEYNHCVPGVLKNAIDWASR
                     PPRRSVLTDKPVAIMGASSGRGATARAQAHLRDGLAYTGALVLPLPEVLVAVAGEKYD
                     EAGELTDQQTRQDIRDLLVALAAWTRRMRQSQDVAAG"
BASE COUNT          366 a          856 c          825 g          352 t
ORIGIN      
        1 gccccggtcg cgacgcgacg ccactcgtgg agcaccgcga cgggatcgcg ggcgaagttg
       61 atggcgtgca ccgcgatcac cacgtcgaag gcgccatcct cgaacggcag ctcaccgaag
      121 tcggcctcga cgaactcggc gtcgggcacg cgacgtcggg caatgtccag catgccggcg
      181 gcggcgtcgg cgccgaccac ccgacgcccc tcccccaccg cggccgccgc gccgttcccc
      241 gtgcccgttc ccacgtccag cacgcgctca ccggcccgca gtgcggcatg ggtcaccacc
      301 cgacgggcaa ccggctcgag ggcgcggccg acggtatcgg cgtactcgcg agccgtcgcg
      361 gagaggacat caacccgcga cggggcgggg gtgccgccgc cggagtcagg cgtggtggcg
      421 ctccacgaac cgctcgatcc ggttcatcgc ctccacgatc tggtcgtagg ccgtggcgta
      481 gcagactcgg acgtgacccg cgcccgaccg accgaacgcg acccccggta cgacgccgac
      541 tcgctcctcc tcgagcagcc gctcggcgaa ctcggtctcg gtcatgccgg tcgcctggct
      601 cacgttcggg aagcagtaga aggcacccat cggctcgaag cagcgcaggc cgatctcgtt
      661 gaaccgatcc gtcatgaagc gacgacggcg gtcgtactcg gcgcgcatgg cctgcaggtg
      721 tggctcgcca ctgcgcagcg cctccattcc ggcgaactgg gccggcgtcg gcgcgcacat
      781 gatcccgtac tggtgcacct tggcgatccc ggtcatcagc ccttcgggcg ccgccacgta
      841 gccgatgcgc cagccggtca tcgcgtagct cttgctgaag ccgcccagca ggacggtccg
      901 gtcacgcatg cggggcagcg agctgaaggc cgtgtgccga tgctccccgt acaccagccg
      961 gtcgtagatc tcgtcgctga agacgaccag gtcatgcgcg tcggcgaccc gcgcgatggc
     1021 ctcgagctgc tcccgatcga gcacggcccc ggtcgggttg ttcggatagc cgaggaagat
     1081 ggccttggtg cgcggcgtga ccgccgcctc gaccatctcg gcggtcaggc ggaagtcggt
     1141 cgcgagtgtc gtgctgaccg gcaccgccac gccgcccgcg agctggatgc acggcgcata
     1201 ggccacgaag cacggctcgt ggtagatgac ctcgtccccc gggttcacga tcgcccgcag
     1261 cgccgcgtcg acgccctccg aggcaccgac cgtgatcacc agctcggtcc gcgggtcgta
     1321 ctcgacgccg taccgatcgc gcaggttggc ggcgatcagc tcgcgcagcg ccagcatccc
     1381 gccattggcg gtgtagtgcg tctcgcccgc ctcgaggctt tcgatggccg cccgcgtgat
     1441 cggctccggc gtggtgaagt ccggctcgcc gatggtgagg ctgatcacgt ccttcatctc
     1501 ggccagcatg tcgaagaagc gccggatgcc ggacggcggc atggtgcgga cggtgtcgtt
     1561 gaggaagtcc gcgggagcgc ggggggtcgg cggacgcgtc gtcatcggtc agtcagtgta
     1621 gtcggacgct tagccagcgg cgacgtcttg ggattgccgc atacgcctgg tccacgccgc
     1681 aagggcgacg agcagatccc gaatgtcctg ccgcgtctgc tggtcagtga gctcccccgc
     1741 ttcgtcgtac ttctccccgg cgaccgcgac cagcacctcg gggaggggaa gaaccagcgc
     1801 ccccgtatag gcgagaccgt cgcggagatg ggcctgcgcc cgggccgtcg cgccacggcc
     1861 ggagctggcg cccatgatcg ccaccggctt gtcggtgagc accgaacggc gcggcggacg
     1921 ggacgcccag tcaatggcgt tcttgaggac cccgggcacg cagtggttgt actcgggcgt
     1981 ggcgatcagc agcgcgtcgg caccgccgat cgcgtccttg aaggcgacca ccgcggccgg
     2041 atccccgtcg gcctccacgt cagcgttgaa cagtgggatc tggtcgagct cgtgggtgac
     2101 gacccgaacc ccgggtggcg cgacctccgc ggcggcacgg atcagcccgc ggttgtacga
     2161 ccctgcgcga agactgccgg ccacgcccag aacggtgagc gggtctcgag cgctgcgtgc
     2221 catttcaatg ccctcatgcg actgacgatg acaacggcat ctgcaagccg ccggccgatc
     2281 gagctctcgg gcaactccga cgacgcgggc cggtatgccg ggccctcacg agccgatgtc
     2341 ctgtcgctta cccgcgcgtg aacgccgaac aggcaaactc acagcgtttc gggaacgac
//
LOCUS       DASTYP010000144         9835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_156145, whole genome shotgun sequence.
ACCESSION   DASTYP010000144 DASTYP010000000
VERSION     DASTYP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156145"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VFV59_06480"
     CDS             <1..811
                     /locus_tag="VFV59_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU5325511.1"
                     /translation="SREGARAAGWPEAVEARAADVTTGDGLADALRGADAAVHLVAIP
                     RESGGRTFAEVNVRGTQRAVEAASKAGVRRFVHLSALGVVDDPRLAYLYSKWLGEQVV
                     QDSDLDWVILRPSLLFGPGDGFFNLIKTTLTWWSPGLVAIPGRGDARFQPLSVEDLAL
                     AVERSVVDADHSRIVHELGGPDYWTYREIVDEVMRVTGKRRIPLPMPIPLISGLTVVT
                     DRVLPIFPVSHDQISSLQRPNFTKLDAFETAFGVTPRPMDLTYLAGSPHPA"
     gene            855..1154
                     /locus_tag="VFV59_06485"
     CDS             855..1154
                     /locus_tag="VFV59_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5325512.1"
                     /translation="MAVGNDPNLLVLVSLAAGPRHGHAILLDVREYAGVRLGPGTLYG
                     AIARLEVDGLIAPMPPEGRRRPYRLTAHGRAELQRRLGGLQAAVRTGLRRLEGTA"
     gene            1151..1720
                     /locus_tag="VFV59_06490"
     CDS             1151..1720
                     /locus_tag="VFV59_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325513.1"
                     /translation="MSRLGRIAARLAIRAYPAAWRRRYGAEIHDLIDESDSSLPDAAD
                     LARAAVREHVAGGEPMRIAPAYRHPVPFAIAAAAILAPTLAMVALSLIGHELGVAAVG
                     TVVDGWLRWVDRVRPVDLALVIAPLVAFMLAVLPLLDLRVERMDGAPAVAVRLRALTA
                     NLLIGALALLVGAALVWHILVESVLRVGP"
     gene            complement(1717..2190)
                     /locus_tag="VFV59_06495"
     CDS             complement(1717..2190)
                     /locus_tag="VFV59_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325514.1"
                     /translation="MAGWRAAYAHILPAAFLAGLSVADRAGRWRSRLGAAALPDAPTL
                     VAVDAEDAVVGFAHTGPLRDADLPAEGRAEIYTLYVHPGAWRAGIGRALVDEVEAFCL
                     ARDMDELFAWVFEANHAARAFYAAVGFVPDGATQIDDFGGVQPVEVRYRRSLRGP"
     gene            complement(2244..2960)
                     /locus_tag="VFV59_06500"
     CDS             complement(2244..2960)
                     /locus_tag="VFV59_06500"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5325515.1"
                     /translation="MANLRITRLGHGGVLYRSPEDAWIWVDRWTGAPNFADAYRTPEK
                     VDVVAPTHCHFDHVGDDCADLVELASVEGAVTIGSHEMSVWLGARGIEAVGMNKGGTF
                     EAAGIRFTMVHADHTGGATLGTGAEQVTRDLGCWGWVIEFEDGTTVYHSGDTDLFGDM
                     NLVRERHHPEIAVLPIGGHYTMGPADAGRALDLIGASLVVPVHYGTFPILAGTPEQLR
                     DATSAEVVELEPGETWEQED"
     gene            3009..3131
                     /locus_tag="VFV59_06505"
     CDS             3009..3131
                     /locus_tag="VFV59_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325516.1"
                     /translation="MPPEPYQTPAGRAARYLVIAAVAAAVLLLIALVVAISLGG"
     gene            complement(3128..3679)
                     /locus_tag="VFV59_06510"
     CDS             complement(3128..3679)
                     /locus_tag="VFV59_06510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020103931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEU5325517.1"
                     /translation="MLRGELYIADDPELEADAARCDALLARFNATRATDVDERRRILT
                     ELLGDLGEGTVIRPPLVMDYGYQTSIGPRTFINSGCVILDVARVRIGADVQVGPNVQL
                     LTPTHPIEAGPRRDKLEAAEPITIGDNAWLGGGVIVLPGVTIGRDTVVGAGAVVTKDL
                     PPGVVAVGNPARVVRALPADSAD"
     gene            complement(3697..4398)
                     /locus_tag="VFV59_06515"
     CDS             complement(3697..4398)
                     /locus_tag="VFV59_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5325518.1"
                     /translation="MTDETVVERSSSPAADPDHGIRIEVGVFGPWQTNSYLVWDGRST
                     DALVLDPGMGAAQPLVERAEANGLRVHLIANSHGHIDHIFDDAPLMEATQAPLAIHPD
                     DAYRLEGRNTYGFDLRAVTASRELRESEQVHIGDLVFDVLHTPGHTEGSVCLYEERLG
                     LLLSGDVLFAGSYGRTDLAGGNDEQMVASLARLVRDIPPTVRVLPGHGPETTLQRELP
                     WLRRIAEAGRLLVPG"
     gene            complement(4395..5231)
                     /gene="lipB"
                     /locus_tag="VFV59_06520"
     CDS             complement(4395..5231)
                     /gene="lipB"
                     /locus_tag="VFV59_06520"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU5325519.1"
                     /translation="MTTNTPFRDISMPPGDAGSDPATIGRPRRPDPTAPYRPASDEHP
                     LLAAHGGTLTVDRLGTVPYGPTWELQDELAEQRRGRRIGDRLLLLEHFPVYTLGRGGD
                     ETNLLASPDRLRELGAELYRIDRGGDITFHGPGQLVAYPIVELRDPLDLRRYVLTLEA
                     AVIATAARFGVTGAHLTGHPGIWVDGERKLCAIGVRVKRGVTTHGLALNVNTDLRWFD
                     EMIPCGIRGKGVTSLARELGRQMPMDEVEDELARQLATHVGLRLAKGAPAVAGPAGGR
                     EQ"
     gene            complement(5435..6502)
                     /gene="lipA"
                     /locus_tag="VFV59_06525"
     CDS             complement(5435..6502)
                     /gene="lipA"
                     /locus_tag="VFV59_06525"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5325520.1"
                     /translation="MALDSTRPNSPSAFSELGELTVVSNPVGGHATPSDVMYPGGPQM
                     DGGLLAPGDPIPENAGPGGRRPEWLKVRVGVGGTYAGTKSIVKGGALNTVCEEARCPN
                     IGECWALGTATIMILGSVCTRNCGFCAIDTGRPPITDLDEPRRVAEAVAKMNLQHVVI
                     TMVARDDLPDGGAEVVADTIRQIREHNPMTGIEVLISDLNGREHDIRTVVEARPDILG
                     HNVETVPRLQRPVRRRARWDRSVFVLTRGAEVAREIGFGQMVTKTGIMVGLGETWDEL
                     LDSMRLLREADVDVLTLGQYLRPSRQHLPLMKYYTPAEFMALRRIGLEMGFRHVQSGP
                     LVRSSYHAAEQVPGRVTAEAV"
     gene            complement(6591..7841)
                     /locus_tag="VFV59_06530"
     CDS             complement(6591..7841)
                     /locus_tag="VFV59_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5325521.1"
                     /translation="MRGTPDPRTVPLPDSAAPTRADALALDAADPLAAFRGRFAVTDP
                     DRIYLDGNSLGALSVAVRDGIAATVDAWGRQLVQGWHEWIELPARIGDRLARTVLGAQ
                     PGEVLVADSVTVNLYKLAHAAADLRPGPLVTDADNFPTDRYVLEGVARQLGRGYVEAS
                     SVDAAVSAAAGGVLVLSLVDYRTAAVLDQARITAATDALVIWDLSHAVGAIEVDLSPA
                     DLAVGCTYKYLNAGPGAPAFLYVRGDLQSQLRSPIQGWFAQREQFAMASGFDPVDGIE
                     RFAAGTPSVVAMAGIEGSLEIVEEAGMPTIAAKGRDLTRLAVELADAWLAPLGFALAS
                     PRDERIRGAHIALRHPQAWPITRALIERADVVPDFRPPDLVRLGFSPLTTRFVDVWDA
                     LDRLRGLVERGEHLRVAAARRRVT"
     gene            complement(7927..9414)
                     /locus_tag="VFV59_06535"
     CDS             complement(7927..9414)
                     /locus_tag="VFV59_06535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5325522.1"
                     /translation="MATTIKMPQLGESVTEGTIERWLVKEGDTVEQYDPLFEVVTDKV
                     NAEVPAEVAGTISKILIGDGETVAVGTAVAEIETDGAGAAAEAPAAEPAPQAEQPAQA
                     EATASAGAEAVAQQEEGPSQAPAAETPSAEAAPPAAAPAAAETPQAQPTGEPVTGEGT
                     ARMTPAVRRLVREHGIDVSQLAGSGAGGRVTREDVLAFIEKGAVSPAPAAEAPSAPQA
                     QPPAAAPAPAAASAPQAQPQAAPAAPPPAAAPKPMAVPAPTGGDVEVPLSQMRKGIAR
                     KMTQVKQTVPHAYTVVEVDMSNVVKWREANNMAFKAREGASLSYVAVVIKAVTETLRK
                     HPTLNSQFAEDKIILKQAMNIGIAVAVDNGLIVPVIANADQLSVSGVNAKIRDLSSRA
                     HANKLRLDELQGGTFTVNNTGWFGSVTSMPIVNAPEVAILSMEAIVKRARVVELDGED
                     IIAIRPMMNMVCSFDHRVLDGAQVGFFLADVRKALEEWDINTSVG"
     gene            complement(9457..>9835)
                     /locus_tag="VFV59_06540"
     CDS             complement(9457..>9835)
                     /locus_tag="VFV59_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5325523.1"
                     /translation="HLSIFCYGYMRTVCLEAAETLAREDGVEAEVIDLRTLRPLDLET
                     VLGSVQKTGKALVVHEANRFGGFGAEIAAEIAEKGFEHLDGPLTRLAGPEVPGVPYHH
                     TLEDWFMIGPARVVDAARRLAAY"
BASE COUNT         1304 a         3506 c         3567 g         1458 t
ORIGIN      
        1 gtcgcgggag ggcgcacggg cggccggctg gcccgaggcc gtcgaggccc gggccgccga
       61 tgtcacgacg ggcgacggac tggccgatgc gcttcgcggc gcggatgcgg cggtccacct
      121 cgtggcgatc ccgcgcgagt cgggcgggcg gacgttcgcc gaggtcaacg tgcgcggcac
      181 gcagcgggcg gtggaggctg cttcgaaggc tggtgtgcga cggttcgtcc acctgtcggc
      241 cctcggcgtc gtcgacgatc cgaggctggc gtacctgtat tcgaagtggc tgggcgagca
      301 ggtcgtgcag gacagcgacc tcgactgggt catcctgcgg ccctcgctgc tgttcggacc
      361 cggcgacggg ttcttcaacc tgatcaagac cacgctgacg tggtggtcgc ccggtctggt
      421 ggccatcccc ggccgagggg acgcccgctt ccaaccgctg tcggtcgagg acctggcgct
      481 ggcggtggag cgttccgtgg tcgacgccga ccactcgcgg atcgtccacg agctcggcgg
      541 ccccgactac tggacctacc gcgagatcgt ggacgaggtg atgcgggtga ccggcaagcg
      601 ccgcatcccg ctccccatgc cgattccgct gatcagcggg ctcaccgtcg tgaccgaccg
      661 cgtcctgccc atcttcccgg tcagccacga ccagatcagc tcgctccagc ggccgaactt
      721 cacgaagctg gacgcgttcg agaccgcctt cggcgtgacc ccgcgaccga tggacctcac
      781 ctacctggca ggctcgccgc accccgcttg acggcggcgg gatactacgt gacacgtagt
      841 agtggttctc gcccatggcc gtcgggaacg atccgaacct cctcgtcctg gtcagcctcg
      901 ccgcaggccc tcggcacggc cacgcgatcc tgctcgacgt gcgcgagtac gccggcgtcc
      961 ggctcgggcc gggaacgctg tacggggcca tcgcgcggct ggaggtcgat ggcctgatcg
     1021 cgccgatgcc gcccgagggg cggcggcgtc cctaccggct caccgcccat gggcgggccg
     1081 agctccagcg acggctgggt ggcctgcagg ccgcggtgcg aacgggcctg cggcgcctgg
     1141 agggaacggc gtgagtcgcc tgggccgcat cgcggccaga ctcgccatcc gcgcctaccc
     1201 cgcggcctgg cgacgccggt acggtgccga gatccacgac ctgatcgacg agagcgacag
     1261 ctccctcccc gatgccgccg acctggcgcg cgcggccgtc agggaacatg ttgctggagg
     1321 tgaaccgatg cgcatcgcgc ccgcctaccg ccatcccgtg ccgttcgcga tcgccgcggc
     1381 cgcgatcctg gcaccaaccc tcgcgatggt ggcgttgtcc ctcatcggcc acgagcttgg
     1441 cgtggcggcg gtcgggacgg tcgtcgacgg ttggctgcgg tgggtcgacc gcgtccgccc
     1501 tgtcgacctc gcgctggtca tcgccccgct ggtagcgttc atgctcgccg tgctgcccct
     1561 cctcgacctc cgcgtcgagc ggatggacgg cgcgccggcc gtggccgttc ggctcagggc
     1621 gctgaccgcc aacctgctga tcggcgccct ggcgctgctc gtcggggcgg cgctcgtctg
     1681 gcacatcctg gtggagtcgg tcctgcgcgt cgggccctag ggaccgcgta gcgagcgccg
     1741 gtagcgcacc tcgaccggtt gcaccccacc gaagtcgtcg atctgggtcg ccccgtcagg
     1801 aacgaagccg acggccgcgt agaacgctcg ggccgcatgg ttcgcctcga acacccaggc
     1861 gaagagctcg tccatgtcgc gtgccaggca gaatgcttca acctcgtcca ccagcgctcg
     1921 cccgatgccc gcgcgccacg cgccgggatg gacgtagagg gtgtagatct cggcccggcc
     1981 ctccgccggc aggtccgcgt cgcgcagcgg tccggtgtgg gcgaagccga cgacggcgtc
     2041 ttccgcgtct accgccacca aggtcggtgc gtcgggcagc gccgccgccc ccaaccgcga
     2101 gcgccagcgg ccggcgcgat cggcgaccga gaggccggcg aggaaggcgg ccggcaggat
     2161 gtgggcgtag gcggcgcgcc agccggccac ctggatcccc gcgatcgcat ccgcgtcgcc
     2221 ctcgcgggca agtcggaccc tcgtcagtcc tcctgctccc aggtctcacc gggctcgagc
     2281 tcgacgacct cggccgaggt ggcgtcgcgc agctgctccg gcgtgcccgc caggatgggg
     2341 aaggtgccgt aatggaccgg aacgaccaat gacgcgccga tgaggtcgag ggcgcgcccc
     2401 gcatcggccg gccccatcgt gtagtgcccg ccgatcggca gcaccgcgat ctcggggtgg
     2461 tgccgctcgc gcaccaggtt catgtcgccg aagaggtcgg tgtcgccgga gtggtagacg
     2521 gtcgtgccgt cctcgaactc gatgacccag ccccagcaac ccaggtcgcg cgtcacctgc
     2581 tcggcaccgg tgccgagcgt cgcgccgccg gtgtggtcgg cgtgaaccat ggtgaagcgg
     2641 atgccggccg cctcgaaggt cccgcccttg ttcatgccca ccgcctcgat gccgcgtgcg
     2701 ccgagccaga cgctcatctc gtgcgacccg atggtgaccg cgccctcgac actcgcgagc
     2761 tccaccaggt cggcgcagtc gtcaccgacg tggtcgaagt gacagtgggt cggcgcgacg
     2821 acgtcgacct tctccggcgt tcggtaggca tcggcgaagt tcggggcgcc ggtccatcgg
     2881 tcgacccaga tccaggcgtc ctcgggtgag cggtagagga cgccgccatg ccccaggcgg
     2941 gtgattcgca gattcgccat cggttcctcc ttgtcgtggg gccatcgtag agcgacgatg
     3001 cgtggaccat gccacccgag ccgtaccaga cgcctgccgg ccgcgcggcg cgttacctgg
     3061 tgatcgccgc cgtggcggcc gcggtgctgc tcctcatcgc gctcgtcgtg gcgatctcgc
     3121 tcgggggcta atccgccgag tcggcgggga gcgcacggac cacgcgggcc gggttcccga
     3181 ccgccaccac gccgggcggc aggtccttcg tgacgacggc cccggccccg acgacggtgt
     3241 cgcgcccgat ggtcacaccg ggcagcacga tcacgccgcc ccccagccag gcgttgtccc
     3301 cgatcgtgat cggctctgcc gcctccagct tgtcgcgacg tggccccgcc tcgatcggat
     3361 gcgtcggcgt gagcagctgc acgttcgggc cgacctgcac gtcggcgccg atgcggacgc
     3421 gcgccacgtc gaggatcacg cagccggagt tgatgaaggt ccgcgggccg atcgaggtct
     3481 ggtagccgta gtccatcacc agcggaggcc ggatgacggt cccctcgcca aggtcgccca
     3541 gcagctcggt gaggatccgc cgtcgctcgt cgacatcggt ggcgcgggtg gcgttgaagc
     3601 gggccagcag cgcgtcacag cgtgcggcat cggcctcgag ctcggggtcg tccgcgatgt
     3661 agagctcgcc gcggagcatc cgctccttct gctcgctcac cccgggacca gcaggcgacc
     3721 ggcctcggcg atccgccgca gccacggcag ctcgcgctgg agcgtcgtct cggggccgtg
     3781 accggggagg acgcggaccg tgggtgggat gtctcgcacc agacgggcga gggaggcgac
     3841 catctgctcg tcgttgcccc ccgcgaggtc ggtccggccg tacgagccgg cgaagaggac
     3901 gtcgcccgac agcagcaggc ccaggcgctc ctcgtagagg cagacggagc cctcggtgtg
     3961 gcccggggta tggaggacgt cgaagaccag gtcgccgatg tggacctgct cgctctcgcg
     4021 aagctcccgg ctggcggtca cggcgcgcag gtcgaagccg tacgtgttgc gtccctccag
     4081 gcgataggca tcgtccggat ggatggcgag cggcgcctgg gtcgcctcca tgaggggcgc
     4141 gtcgtcgaag atgtggtcga tgtgcccgtg cgagttggcg atgaggtgca cccgcagacc
     4201 gttcgcctcc gcccgctcca caagcggctg cgccgcgccc atccccgggt cgaggaccag
     4261 cgcatcggtc gaccgtccgt cccagaccag gtaggagttc gtctgccacg gaccgaagac
     4321 gccgacctcg atccggatgc catggtcggg gtcggcggcg gggctcgagg agcgctcgac
     4381 gaccgtctcg tccgtcattg ctctcgcccg cccgccggtc cggcgaccgc gggcgcgccc
     4441 ttggccaggc gcagcccgac gtgggtggcc agctgccggg cgagctcgtc ctcgacctcg
     4501 tccatcggca tctggcggcc cagctctctg gccagggagg tgacgccctt gccccggatg
     4561 ccgcagggga tcatctcgtc gaaccagcgc aggtcggtgt tcacgttcag ggccaacccg
     4621 tgggtggtca cgccacgctt cacccggacg ccgatggcgc acagcttccg ctcaccgtcg
     4681 acccagatcc cggggtggcc cgtgaggtgg gcgccggtca cgccgaagcg tgccgcggtg
     4741 gcgatcacgg ccgcctcgag cgtcaggacg taccgccgca ggtccagggg gtcgcggagc
     4801 tcgacgatcg ggtaggccac cagctgcccg gggccgtgga aggtgatgtc gccgccgcgg
     4861 tcgatgcggt acagctcggc gccgagctcg cgcagccggt cagggctcgc caacaggttc
     4921 gtctcgtcgc cgccacggcc gagcgtgtag accgggaagt gctcgagcag cagcagccga
     4981 tccccgatgc gccgcccgcg ccgctgctcg gccagctcgt cctggagctc ccacgtcggg
     5041 ccgtacggca cggtgccgag ccggtccacg gtgagggtgc cgccgtgggc ggccaggagc
     5101 gggtgctcgt cgctggccgg tcggtagggc gccgtcgggt ccggacgcct gggacgcccg
     5161 atcgtcgccg gatcgctgcc cgcgtccccg ggaggcatgc tgatgtcccg gaagggcgtg
     5221 ttcgtcgtca tcggctggcg aaccgtccga attcacaccc gatgtgaact acacgggccc
     5281 gattggtgct atcgctgcca ggcgcgccga cgaaccactc cgattcacgc ctcatatgca
     5341 tgagggcccc gtgagaggtc tgacctgccc aacgcctcgt ccgtcgcatg gcgacccttc
     5401 gcatcggccg tggtccgagc cgaacacggc cggctcagac cgcctcggcg gtgacccggc
     5461 cgggaacctg ctcggcggcg tggtagctcg agcgcaccag cggcccggac tgcacgtgcc
     5521 ggaatcccat ctcgagcccg atccgccgca gcgccatgaa ctcggccggc gtgtagtact
     5581 tcatcagcgg cagatgctgt cgcgaggggc gcaggtactg cccgagggtc agcacgtcca
     5641 cgtccgcctc gcgcaggagg cgcatcgagt cgagcagctc gtcccacgtc tcgccgagcc
     5701 cgaccatgat gccggtcttg gtgaccatct ggccgaagcc gatctcgcgg gccacctcgg
     5761 cgccacgggt cagcacgaag accgaccggt cccagcgcgc gcgccgccgg accgggcgct
     5821 ggaggcgcgg gaccgtctcg acgttgtggc cgaggatgtc gggacgggct tcgaccaccg
     5881 tccggatgtc gtgctcacgg ccgttcaggt cgctgatcag cacctcgatc ccggtcatcg
     5941 ggttgtgctc gcggatctgg cggatcgtgt cggccacgac ctcggcaccg ccatcgggca
     6001 ggtcgtcgcg ggcgaccatg gtgatgacga cgtgctggag gttcatcttg gccacggcct
     6061 cggcgacgcg gcgcggctca tccaggtccg tgatcggcgg gcggccggtg tcgatggcgc
     6121 agaagccgca gttccgggtg cagacgctgc ccaggatcat gatggtcgcc gtccccagcg
     6181 cccagcactc gccgatgttc ggacagcgcg cctcctcgca gacggtgttc agggcgccgc
     6241 ccttgacgat gctcttcgtg ccggcgtagg tgccgccgac cccgacgcgg accttcagcc
     6301 actccgggcg acggccgccg ggtccggcgt tctccgggat ggggtcgccc ggagcgagca
     6361 gcccgccgtc catctgcggg ccgcccgggt acatcacatc cgacggcgtc gcgtgccccc
     6421 cgaccggatt gctgaccacg gtcagctcgc cgagctcgct gaacgcggag ggggaattcg
     6481 ggcgggtcga gtccagggcc atgctcgaag tctaccgaag gaccgatgcg cacgcccccg
     6541 ccgccacggc cccgcgaaag ccccgtgcgc ccgcggcggt ggagcggcgg ctacgtgacg
     6601 cggcggcgcg cggcggccac ccggaggtgc tcgccgcgct cgaccaggcc tcgcaagcgg
     6661 tcgagggcgt cccagacgtc gacgaagcgg gtggtcagcg gggagaagcc gagccgaacg
     6721 aggtccgggg ggcggaagtc cggcacgacg tcggcccgct cgatgagggc ccgggtgatc
     6781 ggccaggcct ggggatgcct gagggcgatg tgcgcgccgc ggatccgctc gtcacgcggt
     6841 gacgcaaggg cgaagccgag cggcgccagc caggcatcgg caagctcgac cgccagccgg
     6901 gtcaggtccc gtcccttggc cgcgatggtc ggcatccccg cctcctccac gatctccagg
     6961 ctgccctcga tgcccgccat ggcgacgacc gacggcgtcc cggccgcgaa gcgctcgatg
     7021 ccgtcgaccg ggtcgaaccc gctggccatg gcgaactgct cgcgctgggc gaaccagccc
     7081 tggatcggcg agcggagctg cgactgcagg tcgccacgga cgtagaggaa ggcgggcgcc
     7141 ccggggccgg cgttgaggta cttgtaggtg cagccgaccg cgaggtcggc gggcgacagg
     7201 tcgacctcga tggctccgac cgcgtggctc aggtcccaga tgaccagcgc gtcggttgcc
     7261 gccgtgattc gtgcctggtc gagcaccgcg gccgtccggt agtcgaccag cgagagcacg
     7321 agcacgccgc cggccgccgc cgacaccgcg gcatcgacgg acgacgcctc gacgtacccg
     7381 cggcccagct gcctcgcgac gccctcgagc acgtagcggt cggtggggaa gttgtccgca
     7441 tcggtcacga gcgggccggg ccggaggtcg gcggcggcgt gggcgagctt gtacaggttc
     7501 accgtcaccg agtcggccac gagcacctcg cccggctgcg cccccaggac cgtgcgggca
     7561 agccggtcgc cgatccgcgc cggcagctcg atccactcgt gccagccctg caccagctgc
     7621 cgtccccacg cgtcgacggt cgcggcgatc ccgtcccgga cggcgaccga gagggcgccg
     7681 agcgagtttc cgtcgaggta gatacggtcg ggatccgtga cggcgaagcg gccacggaag
     7741 gccgcgaggg gatcggcggc gtccagcgcg agggcgtcgg cgcgggtcgg ggcggcggag
     7801 tcgggcagcg gaacggtcct cggatcgggc gtgccgcgca ttgtaggagc gcgacgctcc
     7861 tgagaccgcg cgacgcccgg gcggtgagcc cgggcgtccg tatcggtgga tcagccggtc
     7921 agccggtcag ccgaccgagg tgttgatgtc ccattcctcg agcgccttgc ggacgtcggc
     7981 aaggaagaat ccgacctggg cgccgtcgag gacgcggtgg tcgaagctgc agaccatgtt
     8041 catcatcggc cggatggcga tgatgtcctc gccgtcgagc tccacgacgc gcgcccgctt
     8101 cacgatcgcc tccatggaca ggatggcgac ctcgggggcg ttgacgatcg gcatggaggt
     8161 gaccgatccg aaccatccgg tgttgttcac cgtgaaggtg ccgccctgca gttcgtcgag
     8221 ccgcagcttg ttggcgtggg cccggctcga gaggtcgcgg atcttggcgt tcacgccgct
     8281 gacgctcagc tgatcggcgt tggcgatgac cggcacgatg aggccgttgt cgacggccac
     8341 cgcgatgccg atgttcatcg cctgcttgag gatgatcttg tcctcggcga actggctgtt
     8401 cagcgtcgga tgcttgcgca gcgtctccgt tacggccttg atgaccacgg ccacgtagct
     8461 cagcgacgcg ccctcgcgag ccttgaaggc catgttgttg gcctcgcgcc acttgaccac
     8521 gttgctcatg tccacctcca cgaccgtgta ggcgtggggg accgtctgct tgacctgggt
     8581 catcttgcgg gcgatgccct tgcgcatctg cgagaggggc acctcgacgt cgccgccggt
     8641 gggggccggc acggccatcg gcttcggggc ggcggcgggc ggcggagcgg ccggcgcagc
     8701 ctggggctgg gcctgcggag ccgatgcagc agccggcgca ggcgccgctg ccggcggctg
     8761 ggcctggggc gccgacggcg cctccgcggc gggcgccggg ctgacggccc ccttctcgat
     8821 gaacgccagc acgtcctcgc gcgtcacgcg cccgccggca ccggaacccg ccagctgcga
     8881 gacgtcgatc ccgtgctcgc gcaccaggcg gcggacggcc ggggtcatcc gggcggtccc
     8941 ctcgccggtt accggctcgc cggtcggctg ggcctgcggc gtctcggcgg cggcgggtgc
     9001 tgccgcgggc ggcgccgcct cggcggacgg cgtctcggcg gcgggtgcct gggacgggcc
     9061 ctcctcctgc tgcgccaccg cctcagcgcc ggcggacgcc gtcgcctcgg cctgggcggg
     9121 ctgctcggcc tgcggggccg gttcggcagc gggtgcctcg gccgcggcac cggcgccatc
     9181 ggtctcgatc tccgcgaccg cggtgccgac ggcgaccgtc tcgccgtcgc cgatcaggat
     9241 cttgctgatc gtgccggcca cctcggcggg cacctccgcg ttgaccttgt cggtgacgac
     9301 ctcgaacagc gggtcgtact gctcgacggt gtcgccttcc ttgaccagcc agcgctcgat
     9361 cgtcccttcg gtgaccgatt cgcccagctg gggcatcttg atcgtggttg ccatcggtat
     9421 gggggccctt ccgtatggtc gcgtcgttgt cttcgtctag taggcggcca ggcgacgggc
     9481 cgcgtcgacg acccgggccg gaccgatcat gaaccaatcc tcgagggtgt ggtggtacgg
     9541 cacgccgggc acctcggggc cggccagtcg ggtgagcggg ccgtccaggt gctcgaagcc
     9601 cttctcggcg atctcggccg cgatctcagc accgaagcca ccgaaccgat tcgcctcgtg
     9661 caccacgagt gccttgcccg tcttctggac cgagccgagc accgtctcca ggtccagcgg
     9721 gcgcagcgtg cgcaggtcga tgacctcggc ctcgacgcca tcctcgcggg ccagcgtctc
     9781 ggccgcctcc aggcagacgg tgcgcatgta gccgtagcag aagatcgaga ggtgc
//
LOCUS       DASTYP010000145        20116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_11030, whole genome shotgun sequence.
ACCESSION   DASTYP010000145 DASTYP010000000
VERSION     DASTYP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 20116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20116
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11030"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /gene="acpP"
                     /locus_tag="VFV59_06545"
     CDS             <1..235
                     /gene="acpP"
                     /locus_tag="VFV59_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5325524.1"
                     /translation="GTTYERLKKIIVEQLGVDEEEVTPEASFVEDLNADSLDLVELIM
                     SLEEEFGMEISDEDAEKIQKVSDATEYIEEHQQ"
     gene            248..991
                     /gene="rnc"
                     /locus_tag="VFV59_06550"
     CDS             248..991
                     /gene="rnc"
                     /locus_tag="VFV59_06550"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU5325525.1"
                     /translation="MEAPSLEKLGERLGIVLRDPAQIRQAFVHSSYHNENPELAPEHN
                     ERLEFYGDAVIGLVVSRLLYDRHPDEDEGFLTARRAALVNREALAVMALELGLDRLLL
                     LGRGEAEGGGARRPSVLAACFEALAGALSLSEGPERTTEVLRRLFTPRLSALSVDTAP
                     KSAKSRFQELTQRVYGQKPVYQLRGSSGPPHLQQFEVAAVLDGRELGTGTGSSRQRAE
                     EQAAAAALEAIEGENVAAAGEGRSASTTP"
     gene            988..4440
                     /gene="smc"
                     /locus_tag="VFV59_06555"
     CDS             988..4440
                     /gene="smc"
                     /locus_tag="VFV59_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU5325526.1"
                     /translation="MSRLRELRLHGFKSFADQTRFVFEPGVNAVIGPNGSGKSNMADA
                     VRWVLGEQSNRSLRTRRADDVIFAGSESRKPQGMAEAVLTLDNEDGWLPIDFAEVSIG
                     RRAYRSGDSEYLINGARARLRDVVELLGEGRLGANELVVVGQGTVDAALSLRPEERRQ
                     LFEEAAGVKGLQVKRNEATVRLAKSLDNLARVSDILGELKPQVRRLALQAEHQQQHDA
                     LGARARALVIASHRRREQQARARLGDARRAAAAAEAALAAFRESQEATRTAVQAAEAR
                     YWEAEEAARAAAHEREATREALIRAESRLENLEARVRELAGTLAREEKELADAEASAI
                     ASPSEARDAIAPALAQAQAAEEAWRMAVARLADADESLLHAEEAVAAVRARQSDRIAA
                     AARLSEEAARHASRVEQLEREAVDLRARSQELADAARRAAAATSAATRRVEEAREALE
                     RAAHERDEAQAAADRARQRAVELDERRRALRAELDAIAEPHDAASRLGRRLRAAGWPS
                     LLDAIDAPEASWPAIEAVVGGELEAALVWHDADPSDAVGDARGAVRLLAAAEPGGADR
                     DAALAAVDASRTLAEWAGGSAAPRAFHRTVVARDLAALLDGWRRLPPGWSAVTEHGDL
                     ADARGIVVLRGRADPPGGAAARIHARRRELTEAVGRLERETATAQAAAHEATERLRAA
                     RDGHLAARDAHDEAERDLRRSHGEEETATAQAERLTTRLSRLSDERAALGPAPAPVDA
                     ATGEASAPELAELESAAVRARAERVRLAAERDTARERWTATREAAQEIETRTAGQRQE
                     RAVREARVAQLRASIPERRAQLETARAEHEEVRQIAATSREADEAARARHETAERARA
                     EQRRELLELERGAGSAGSRLAELERQAQATAIEASRRDEALGALARERELAMEGLPET
                     DAPLPTDEVDALDDEALETELRRVRRTLAQIGSVNPFAVEEHREMAARLDEMTTQDAD
                     LRAAIASTEELIARLDSEITQQFDTAFRAIAEHFDAYCRLLFAGGSGSLQMADEADGD
                     APGGIEIVVRPPGKRLQRLAMLSGGERALTGVALLFAMLTVNPVPFCILDEVDAALDE
                     ANIGRFAQALRRLSESIDFVVITHNRATIEVADTIYGVTMTDAAVSRVLSLRLADLPV
                     EVTA"
     gene            4557..5483
                     /gene="ftsY"
                     /locus_tag="VFV59_06560"
     CDS             4557..5483
                     /gene="ftsY"
                     /locus_tag="VFV59_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5325527.1"
                     /translation="MTVARPRREPAGMGLRGRPSGLGARLRSIFGAGTTATDETWDEV
                     EEALIAADVGATATLELVDAARQRFRADAGRTGEDARRALATEIRDRLARVGSGQFEL
                     GPAPSVVLFVGVNGTGKTTTIAKLAHRLVGQGRSVALAAADTFRAAAVEQLRVWGEQL
                     GVAVIAQRAGADPGAVAFDAVAAAESRNLDAVLVDTAGRLQNKQQLMDELSKIRRVIE
                     RRLPDQPRHVLLVLDATTGQNGLLQAEAFSREAGVTGIVLTKLDGTAKGGVALAVADR
                     LGVPIVFAGVGEEVDALAPFDPEAFVEWLFAE"
     gene            5552..6889
                     /gene="ffh"
                     /locus_tag="VFV59_06565"
     CDS             5552..6889
                     /gene="ffh"
                     /locus_tag="VFV59_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5325528.1"
                     /translation="MFESLSSRLQGITQRLRGKARITEADLDAALREIRLALLEADVN
                     FKVVKEFVARIREQAVGTDLLTGLNPGQQIVKIVHDELVATLGGERHHLRIEGRPGLI
                     MMVGLQGSGKTTTAAKLAVRLRREGKRPLLVAADVYRPAAVDQLVQLGDQVGVEVHTR
                     SVGTPALEVARSGLEAARQRNHDVVILDTAGRLHVDEAMMEELVRISGTVTPSETLLV
                     VDAMTGQESVRVAEAFHARLPVTGLVLTKMDGDARGGAALSIRAATGLPVAFLGTGER
                     TDGLEAFHPDRLAQRILGMGDILSFVERVQENVDREEAERAAQRMMENRFTLEDFRSQ
                     LNQIKKMGPIGQLVGMIPGASAMAGAAQEAVDSGQMKRIEAIIDSMTPEERRRPEIIK
                     ASRRRRIALGSGTSTAEVNRLLKQFVEMQKMMKMLSGGKLPRTGGGLGALLGR"
     gene            6897..8465
                     /locus_tag="VFV59_06570"
     CDS             6897..8465
                     /locus_tag="VFV59_06570"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEU5325529.1"
                     /translation="MHRLVIAATTLLALTGAVVVGAYLFLFAASSDRLATLAPERTAF
                     YVNVYLQPSTGQQMNLAGLIGRIPGFEDTSTLDEKIDQIAENLLSGSGIDYREDVKPW
                     LGNQLAIAAWPGGDQLEAQPVLLAEVKDREAAEASIADVTGGTDFRTEAYGGVDLHVG
                     DGTAYAFVDDRTLVVATQPTDLQAIVDVAGGEADLASRADFRDTMADLPADHLASVFV
                     DVAAIADATDTATQLSEVSTAGAALVAEQDGIRLSGSAPFDMADAGSSSKATFALGTE
                     PSSLVDWMPDDTVAELVVFGLRQTLEDAEAAAQATPQGQDVTSTLDAVRAIAAFGLGV
                     DLDSDILPLLDREVGVAINGFDGDLPTGQLLLRPDDPDAAEQALDRVADGLVNLAGAT
                     RDDVDVDGISVSTLAVPDVPTIAWTRAEGLLILGLRPEDVAAAVRAHESGEALGASEA
                     YRRTFEVAGARAGNEAWIDVQAVVQLLGADADLPQDARDILGQIGAFGFTAPSRSDQI
                     EFHAVVTVDDRPTE"
     gene            8502..8906
                     /gene="rpsP"
                     /locus_tag="VFV59_06575"
     CDS             8502..8906
                     /gene="rpsP"
                     /locus_tag="VFV59_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5325530.1"
                     /translation="MAVRIRLTRVGATKRPAYRVIAIDQRRSRDGRAIEILGYYDPLT
                     EPATVHLETEKIKAWIGKGAQPSETVSKLMRLAEKPAAGTEASDKPKRATRASKPKAS
                     AKKTEDAPAAEPAEASTDSAAEASADAEPTEA"
     gene            8903..9142
                     /locus_tag="VFV59_06580"
     CDS             8903..9142
                     /locus_tag="VFV59_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU5325531.1"
                     /translation="MKEFLEYVARSLVDKPDAVWVEEDQEGDETILTLGVDQEDMGRV
                     IGRDGRIANAIRSLLRVMATRDGRHVELEIVSDAD"
     gene            9099..9632
                     /gene="rimM"
                     /locus_tag="VFV59_06585"
     CDS             9099..9632
                     /gene="rimM"
                     /locus_tag="VFV59_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU5325532.1"
                     /translation="MGATSSSRSSPTPTDRLAVARIIGPKGLEGAVRLELVTDWPEEQ
                     IVVGAALWLDGETEPMRVTRVERGGRVLVVHLDGLVSREAAEAMVGRYLEAPAPSLPA
                     DTYFWHDLIGLRVEDERGAVIGELVEIFRAGGNEVYRVIGPDGERLVPALRSAIRDID
                     LDGGRMVLAPDDAEEVR"
     gene            9641..10369
                     /gene="trmD"
                     /locus_tag="VFV59_06590"
     CDS             9641..10369
                     /gene="trmD"
                     /locus_tag="VFV59_06590"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU5325533.1"
                     /translation="MRIDVVTLFPELIEPALRTSIIGRAAESGRVQFGIHDLREHGLG
                     RHRSVDDYPYGGGAGMVMRPEPLFAAVEPLRAEGAHVVLMDAAGERLTDALARELAGI
                     PHLAFVCGRYEGVDERVRTLVDREVSIGDYVVTGGELPALVAIDAVVRLVPGVIAEAS
                     HADDSFASGLLEYPQYTRPEAFRELAVPPVLLSGNHGEVERWRRREALRRTLERRPDL
                     LHSVELSADDRALLEELERGRPDA"
     gene            10513..10860
                     /gene="rplS"
                     /locus_tag="VFV59_06595"
     CDS             10513..10860
                     /gene="rplS"
                     /locus_tag="VFV59_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5325534.1"
                     /translation="MNAIKELEREQLRTDVPEIAPGDTVRVSVKVVEGNRERVQVFEG
                     TVMRMRGGGLNHQITVRRIASGVGVERTFMVHAPRIDKIEVVRHGVARRAQLYFLRDR
                     VGKSATLRERRSN"
     gene            10907..11602
                     /locus_tag="VFV59_06600"
     CDS             10907..11602
                     /locus_tag="VFV59_06600"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5325535.1"
                     /translation="MRTARPRARDAHMRHERELHLAGFHRVAGIDEVGRGCLAGPVVA
                     AAVILPERHRIRGLRDSKVLGRARREALYAQILDRAEAVGVGCVEVETIDRINILQAT
                     KLAMREALGRLNQPADHLLIDALSLRDVELPQRPIIDGDAISASIAAASIVAKVTRDR
                     ICDEMDRRFPAYGFARNKGYGTRHHVDALLGEGPCEWHRRSFAPIRQLLAGEQLSLEL
                     DFTLVDEATLDPD"
     gene            11630..12001
                     /locus_tag="VFV59_06605"
     CDS             11630..12001
                     /locus_tag="VFV59_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5325536.1"
                     /translation="MADPRHQLGRRAEDAVASWLTRCGWRVLAVRYRLRSIGEVDLIA
                     LDPGDVLVALEVRARRSPRTGAAVATVDSRRISRLARTLAAYAEASRTRHRGLRIDLV
                     AVEPDATAGWRMIRHPAIGER"
     gene            complement(12017..12970)
                     /locus_tag="VFV59_06610"
     CDS             complement(12017..12970)
                     /locus_tag="VFV59_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5325537.1"
                     /translation="MSQRPRLLFPALGLAATLTAGPFLAGVALAADPIVVVQRGDTLS
                     GISKRQGVAISDIVALNGLADPNRIYVGQRLRLTAPAPAAPAAAAPPAPAAPAAATAP
                     STTVHTVARGESLWTIARRYGVSVSAIVAANGISNPSRIYGGQQLTIPGTAPAPAPAA
                     PAATPPAASTPSAPAMPPSMARLVAGRDAMRQLIVAEAQRFGVPPAFALAVAWQESGW
                     RQEVVSSAGAVGVMQLMPATGAWVAEAMLGTPVDINDTTHNVRAGVRLLAHYLQRYQG
                     DRDLVLAAYYQGQYATDRHGIYGVTRPYIASIRLLEVLFGG"
     gene            13328..13987
                     /gene="rpsB"
                     /locus_tag="VFV59_06615"
                     /pseudo
     CDS             13328..13987
                     /gene="rpsB"
                     /locus_tag="VFV59_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634886.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            14312..14935
                     /gene="tsf"
                     /locus_tag="VFV59_06620"
     CDS             14312..14935
                     /gene="tsf"
                     /locus_tag="VFV59_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU5325538.1"
                     /translation="MIKPEDVKRLREQTGAGIMDCKRALEQSGGDYEKAALWLREKGL
                     STAAKKAGRAAREGIIASYIHHGARLGVLLELNCETDFVARTDDFQQLSRDIAMQIAG
                     MSPLYVSRDDVPADVLDEQKRLFAIDAERDGRPADRIPMIVEGKLNKWFEQVCLLEQP
                     YRDTDKKMSDLITEKVALLGENIKVARFARMAVGETADAAEPTDEGA"
     gene            14940..15692
                     /gene="pyrH"
                     /locus_tag="VFV59_06625"
     CDS             14940..15692
                     /gene="pyrH"
                     /locus_tag="VFV59_06625"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5325539.1"
                     /translation="MTVSVTADAKSRESRRVLLKLSGEALMGEHEYGIDPAVVRDIAS
                     QVADSAREGIQVAIVVGGGNIFRGLAASARGMDRATGDYMGMLATVMNGLAIQDALEQ
                     VGCPTRVMSAIAMNEICEPYIRRRAVRHLEKGRVVVMVAGTGNPYFTTDTAATLRAVE
                     VHAQVILKATRVDGVYDADPETHPEAQRFSRIGYTQLLSSRLQALDATAVSLAMDNEM
                     PIVVFDMTVPGNIARAVRGERIGTLISGEESR"
     gene            15689..16246
                     /gene="frr"
                     /locus_tag="VFV59_06630"
     CDS             15689..16246
                     /gene="frr"
                     /locus_tag="VFV59_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5325540.1"
                     /translation="MTHEALTAIEPKMQRAVEAMERDFAGFRTGRASTALVERIVVDY
                     YGAQTPLNQLAGISTPDPHLIVIQPWDRSVLSAIEKAITKSDIGLVPNVDGTVVRLNV
                     PPLTEERRRDMVKQVRRRTEEAKVEVRNHRREVADQLKRSMRDGELSEDDERRELDQL
                     QKLTDRFIEAIDSRADRKEAEILEV"
     gene            16249..16941
                     /gene="uppS"
                     /locus_tag="VFV59_06635"
     CDS             16249..16941
                     /gene="uppS"
                     /locus_tag="VFV59_06635"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5325541.1"
                     /translation="MPEAVAPRHVAIIADGNRRWARDRGLPVLAGHVQGIEAIRPVVR
                     RARDRGVSTLSVYTFSTENWSRPGDEVTGLFGLIDGAVRQYTDELIEEGVRVRVIGRL
                     HEAPADVQRSIRNAEERTQGGTRLDLNICFNYSGRAEIVDAARELIAARVDPDSVDEA
                     HFATHLYTAGQPDVDLLIRTGGDVRTSNFLIWQAAYAELVFCPKFWPEFTPEDFDAAL
                     DDFARRERRFGG"
     gene            16946..17845
                     /locus_tag="VFV59_06640"
     CDS             16946..17845
                     /locus_tag="VFV59_06640"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5325542.1"
                     /translation="MLRTRLLTAAILGPIILGVVLLGEPWLSLLIGLVAFLALVEMVS
                     LLDAAGYQPPQVLSILAGMALTAAGLTAANAQGVGGPAATLLASLDPPGVVASTLVAT
                     VLLLAAAGFMRGDPRAGFLTWAMTTFGVAYVGLLLPTIVLVAHLAPPGGSWTSGVGGL
                     GIGSGVAWALTLVLIVWGYDTGAYLTGRLVGRRRLVEHISPSKTVEGVAGGLILSTIG
                     AGIGAWLIGLEPWHPLIMGPLVGLAAQAGDLAESMLKRAAGRKESGFLVPGHGGILDR
                     VDSFLFAAPMLAGYALLVAGRVV"
     gene            17842..18969
                     /gene="dxr"
                     /locus_tag="VFV59_06645"
     CDS             17842..18969
                     /gene="dxr"
                     /locus_tag="VFV59_06645"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU5325543.1"
                     /translation="MTGVAILGSTGSIGRQALEVIEGLGDRFEVRALASGRGGVGFAR
                     QLERWPAAKAWCAQDEASVPVSRRVSGLAELATLHGVELVVVATTGMTALPAVLDALR
                     SGRRVALANKETLVTGGHLVARELDELGGDPLDRLRPIDSEHSAIWQCVVGEPREAIE
                     RLVLTASGGPFRGRPPESLVDVTAAEALAHPTWRMGPKITIDSATLVNKAFEVIEAKW
                     LYRLPYSRIDAVIHPQSVVHSLVEFADGSFKAQLGLPDMRLPIQYALTYPERTPSPAR
                     RSHPADWGSLAFAPLSPGAFPAYDVVRAAAAAGGNRGTIVNAADEVAVAAFLDGRIGF
                     TAIARTIERSVERWGSDRTPDLHDLIALDGEVRDAVEAELR"
     gene            18969..20096
                     /locus_tag="VFV59_06650"
     CDS             18969..20096
                     /locus_tag="VFV59_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEU5325544.1"
                     /translation="MDVLATVAAFIGVLVVLVLVHELGHFVAAKRSGITVEEFGIGFP
                     PRITSVTWHGTRYSLNWIPLGGFVKMLGEDGDVEIRRLRERGLGDAEIERAMDGAFNR
                     RPVWIRVAVLLAGVAMNFVLAAALFAIAFSMPVPFGKGPLQVASIQPDSPASTSLEVG
                     DVITGADGETFDRSADLTDYINERAGRPVVLQVQRDGNAVDVAVTPRVVSERDRASGI
                     GPVGFGYKPAEVGEDPPIADGPIDAIARGTGFAGTLAVQIPGGLADAVGGLLGLNPNA
                     GDARGPIGIAQETGEVLDSPLFNQLLFVGLLSVNLAVLNVLPFPPLDGGRIAVVLVEA
                     VRRRRLPAEREALIYLTGFAVLIALVILISIQDIQRLITGG"
BASE COUNT         2962 a         7090 c         7363 g         2701 t
ORIGIN      
        1 cgggaccacg tacgaacgac tcaagaagat catcgtcgag cagctcggcg tcgacgagga
       61 agaggtcacg cctgaggcct cgttcgtcga ggacctgaac gccgactcgc tggacctggt
      121 cgagctcatc atgagcctcg aggaggagtt cggcatggag atctcggacg aggacgccga
      181 gaagatccag aaggtctcgg acgccaccga gtacatcgag gagcaccagc agtaacgcgg
      241 tgccttgatg gaggctccct cgctggagaa gctcggcgag cgtctcggca tcgtcctgcg
      301 tgacccggcg cagatccggc aggccttcgt ccacagctcg taccacaacg agaaccccga
      361 gctcgcgccc gagcacaacg agcggctcga gttctacggt gacgccgtca tcggcctggt
      421 ggtcagccgc ctcctctacg atcgtcatcc cgacgaggac gagggtttcc tgacggcgcg
      481 ccgcgccgcc ctggtgaacc gcgaggcatt ggccgtcatg gccctcgagc tcggcctcga
      541 ccggctgctg ctgctcggtc gtggcgaggc cgagggcggg ggagccaggc gaccgtcggt
      601 cctggcggcg tgcttcgagg cgctcgcggg cgccctctcg ctgagtgagg gtcccgagcg
      661 gaccaccgag gtcctgcgcc gcctcttcac cccgcgcctc tcggcgctct cggtggacac
      721 cgctcccaag tcggcgaagt cccgcttcca ggagctcacg cagcgggtct acggccagaa
      781 gccggtctac cagctgcgcg gcagcagcgg tccgccgcac ctccagcagt tcgaggtggc
      841 ggccgtgctc gacggccggg agctcgggac ggggaccggt tccagtcgcc agcgcgcgga
      901 ggagcaggct gcggcggccg cgctcgaggc catcgagggc gagaacgtgg ccgccgcggg
      961 cgaggggcgc tccgcgtcga cgacgccgtg agtcgcctgc gcgagcttcg gctccacggc
     1021 ttcaagtcct tcgccgacca gacccgcttc gtcttcgagc ccggcgtcaa cgccgtcatc
     1081 ggtcccaacg gctcggggaa gagcaacatg gccgatgccg tccggtgggt cctgggcgag
     1141 cagtcgaacc gatccctgcg cacccggcgc gcggacgacg tcatcttcgc ggggtccgag
     1201 tcccgcaagc cgcagggcat ggccgaggcg gtgctgaccc tcgacaacga ggacggctgg
     1261 ctgccgatcg acttcgccga ggtcagcatc gggcggcgcg cgtaccggtc cggcgacagc
     1321 gagtacctca tcaatggcgc gcgagcgcga ctccgcgatg tggtcgaact gctgggggag
     1381 gggcgcctcg gcgccaacga gctggtggtg gtggggcagg gaacggtgga cgccgcgctc
     1441 tcgctccgcc ccgaggagcg gcgccagctg ttcgaggagg cggccggcgt caagggcctg
     1501 caggtcaagc gcaacgaggc gacggtgcgg ctggcgaagt cgctcgacaa cctcgcgcgg
     1561 gtcagcgaca tcctgggcga gctcaagccg caggtccggc gcctcgccct ccaggccgag
     1621 caccagcagc agcacgatgc gttgggagca cgcgcccggg cgctggtcat cgcgtcgcac
     1681 cggcggcgcg aacagcaggc gcgcgcgcgc ctcggcgacg cccgacgcgc cgccgccgcg
     1741 gccgaggcgg cgctggccgc cttccgcgag tcacaggagg ccaccaggac cgccgtccag
     1801 gcggccgagg cccggtactg ggaggcggag gaggccgctc gggcggcggc gcacgagcgg
     1861 gaggcgaccc gcgaggcgct catccgggcc gagagccggc tcgagaacct cgaggcgcgg
     1921 gttcgggagc tcgccggcac gctggctcgc gaggagaagg agctggccga tgcggaggcc
     1981 tcggcgattg cgtcgccgag cgaggcgcgc gacgcgatcg cgccggcgct ggcccaggcg
     2041 caggcggcgg aggaggcgtg gcgcatggcc gtcgcacgcc tggccgacgc ggacgagtca
     2101 ctgctccacg ccgaggaggc ggtggccgcg gtgcgcgcgc ggcagagcga ccggatcgcc
     2161 gccgcggcgc ggctgtcgga ggaggcagca cggcacgcct cccgggtcga gcagctggag
     2221 cgggaggcgg tcgacctgcg cgcccgttcg caggagctgg cggacgccgc ccgccgagca
     2281 gccgccgcga cgtccgcagc gacacgacgg gtcgaggagg cgcgagaggc gctcgagcgc
     2341 gccgcgcatg agcgggacga ggcccaggcg gcggccgacc gggcccgcca gcgggccgtc
     2401 gaactggacg aacgccgccg agcgctgcgc gcggagctcg acgcgatcgc ggagccgcac
     2461 gacgccgcgt cgcggctcgg gcgacgactg cgcgcggccg gctggccgtc gctgctcgat
     2521 gcgatcgacg cccccgaggc ttcgtggccc gcgatcgagg cggtggtcgg cggggagctc
     2581 gaggcggcac tggtgtggca cgacgcggac ccgtccgacg ccgtgggcga tgcccgtggt
     2641 gcggtacggc tgctcgccgc ggcagagccc ggtggcgccg accgcgatgc ggcgctggcc
     2701 gcggtggacg cgtcgaggac gctcgcggag tgggccggcg gatccgccgc gccgcgcgcc
     2761 ttccatcgga cggtcgtggc gcgagacctc gccgcgctgc ttgacggatg gcggcggctg
     2821 ccccctgggt ggagcgccgt gaccgagcat ggcgacctgg cggacgcgcg cggcatcgtg
     2881 gtcctgcgcg gccgtgccga cccgcccggc ggagcggcgg ctcgcatcca cgcacgacgc
     2941 cgagagctga ccgaggcggt cggccgcctg gagcgagaga cggcaacggc gcaggcggcg
     3001 gcgcacgagg cgaccgagcg actgcgtgcg gcacgtgacg ggcacctcgc cgcgcgcgac
     3061 gcgcacgacg aagccgaacg ggacctccgc cggagccacg gcgaggaaga gaccgcgacc
     3121 gcccaggcgg agcgcctcac gaccaggctc tcccgcctga gcgacgagcg agccgccctc
     3181 gggccggcgc ctgccccggt ggatgccgcg acgggcgagg cctcggcacc cgagctcgcc
     3241 gagctcgagt cagcggccgt gcgcgcccga gccgagcgcg tccggctggc cgcggagcgt
     3301 gacacggcgc gagagcgatg gaccgcgacg cgtgaggcgg cgcaggagat cgagaccagg
     3361 accgccgggc agcgccagga gcgggccgtg cgcgaggcgc gcgtcgccca gctgcgggcg
     3421 tcgataccgg agcgacgggc acagctcgag acggcaaggg cggagcacga ggaggtccgg
     3481 cagatcgccg cgacgtcgcg ggaggcggac gaggcggcac gcgcacgtca cgagaccgcc
     3541 gagcgcgccc gggcggagca gcgacgcgag ctgctggagc tcgagcgcgg agccggcagc
     3601 gccggctcgc ggctcgccga gctggagcgg caggcccagg cgacggccat cgaggcatca
     3661 cggcgggacg aggcgctcgg cgccctggcc cgcgagcggg agctcgccat ggagggtctc
     3721 cccgagaccg atgcgccgct gcccaccgac gaggtcgacg cgctcgacga cgaggcgctc
     3781 gagacggagc tgcggcgtgt gcgccgcacg ctggcgcaga tcggatcggt caacccgttc
     3841 gcggtcgagg agcaccgcga gatggcggca cgcctggacg agatgaccac acaggacgcg
     3901 gatctccgcg cggccatcgc ctccaccgag gagctgatcg cgcggctcga ctcggagatc
     3961 acgcagcagt tcgacacggc cttccgcgcc atcgcggagc acttcgacgc ctactgccgc
     4021 ctgctcttcg ccggcggctc gggttccctg cagatggcgg acgaggccga tggcgacgcc
     4081 ccgggcggga tcgagatcgt ggtgcggccg cccggcaagc ggctgcagcg cctggcgatg
     4141 ctgtccggcg gcgagcgcgc cctgaccggc gtcgcgctgc tgttcgccat gctcaccgtc
     4201 aacccggtac ccttctgcat cctggacgag gtcgacgccg cgctggacga ggccaacatc
     4261 ggccgattcg cgcaggccct ccgacgcctg tcggagtcga tcgacttcgt cgtcatcact
     4321 cacaaccgcg ccaccatcga ggtggccgac acgatctacg gagtcaccat gaccgatgcc
     4381 gcggtcagcc gcgtcctctc gctccgcctc gccgacctgc cggtcgaggt gacggcctag
     4441 tgttctggcg ccgccgcgag cgcgcagccg agcccgaggt tcctgtggaa gcggatctca
     4501 cgccgcagcc cgggccacga cccgagccgg agccccagcc gggggtcgcc tcgccgatga
     4561 ccgtcgcgcg ccctcgacgc gagccagccg gcatgggcct gcgcggccgt ccatcggggc
     4621 tcggtgcgcg tctgcgctcg atcttcggcg cgggcacgac ggccaccgac gagacgtggg
     4681 acgaggtcga ggaggcgctg atcgccgcgg acgtcggggc gacggcaacc ctcgagctgg
     4741 tcgatgcggc gcgccagcgg ttccgggcgg acgcggggcg gacgggcgag gacgcgcgac
     4801 gcgcgctcgc caccgagatc cgcgatcgcc tggcccgcgt cggctcaggc cagttcgagc
     4861 tgggtccagc gccgtcggtc gtcctcttcg tgggcgtcaa tggcacgggc aagacgacga
     4921 cgatcgccaa gctcgcgcat cggctggtgg gtcagggccg cagcgtggcg ctggccgcgg
     4981 cggacacgtt ccgtgccgcg gcggtggagc agctgcgcgt ctggggcgag cagctcggcg
     5041 tcgcggtgat cgcgcagcgg gccggtgcgg atcccggggc cgtggccttc gacgccgtgg
     5101 ccgccgccga gtcgcgcaac ctcgacgcgg tgctggtcga cacggccggc cggctgcaga
     5161 acaagcagca gctgatggac gagctgtcca agatccggcg cgtcatcgaa cgccggcttc
     5221 cggaccagcc ccgccacgtc ctgctcgtcc tggacgccac gaccggccag aacgggctgc
     5281 tccaggccga ggccttcagc cgtgaggcgg gggtgaccgg catcgtgctg accaagctgg
     5341 acggcaccgc caagggtggc gtggccctcg ccgtggcgga tcggctcggg gttccgatcg
     5401 tcttcgccgg cgtcggcgag gaggtcgacg cgctggcacc gttcgacccc gaggccttcg
     5461 tcgagtggct cttcgccgag taggcgccta gaatgggcag ccgtttgccg gtcgtgccag
     5521 gccggccccg ccatccccgt actggttgag catgttcgaa tccctgtcgt cgcgcctgca
     5581 gggcatcacc cagcgcctgc gcgggaaagc ccgcatcacc gaagccgatc tcgacgccgc
     5641 gctgcgcgag atccggctcg cgctgctcga ggccgacgtc aacttcaagg tcgtcaagga
     5701 gttcgtggcg cgcatccgcg agcaggcggt cggcacggat ctgctgaccg gcctgaaccc
     5761 ggggcagcag atcgtcaaga tcgtgcacga cgagctcgtg gcgacgctgg gcggcgagcg
     5821 gcaccacctc aggatcgagg gccggccagg cctgatcatg atggtcggcc tgcagggctc
     5881 cgggaagacg accaccgcgg cgaagctggc ggttcgcctc cgccgcgagg gcaagcgtcc
     5941 gctgctggtc gccgccgacg tctaccgccc tgccgccgtc gaccagctgg tgcagctcgg
     6001 cgaccaggtc ggcgtcgagg ttcacacgcg ctcggtcggg acgccggccc tggaggtggc
     6061 acggtccggc ctcgaggcgg cccggcagcg caaccacgac gtggtgatcc tcgacaccgc
     6121 gggccggctc cacgtcgacg aggcgatgat ggaggagctc gtccgcatct ccggcaccgt
     6181 gacgccatcg gagacgctgc tggtcgtgga cgccatgacc ggccaggaat cggtccgcgt
     6241 cgcggaggcg ttccacgcgc gcctgccggt gaccggcctg gtgctgacca agatggacgg
     6301 cgatgcacgc ggcggcgcgg cgctcagcat ccgtgccgcg accgggctgc cggtcgcctt
     6361 cctcggcact ggcgagcgca ccgatggcct cgaggccttc cacccggatc gcctggcgca
     6421 gcgcatcctg ggcatgggcg acatcctcag cttcgtcgaa cgcgtccagg agaacgtcga
     6481 ccgcgaggag gccgagcggg ccgcccagcg catgatggag aaccggttca cgctcgagga
     6541 cttccgcagc cagctgaacc agatcaagaa gatggggccg atcggccagc tggtcggcat
     6601 gatccccggc gccagcgcca tggccggcgc ggcccaggag gcggtcgact cgggccagat
     6661 gaagcgtatc gaggcgatca tcgactccat gacgcccgag gagcggcgcc ggcccgagat
     6721 catcaaggcg tcccgccggc ggcgcatcgc gctgggcagc ggcacctcga cggccgaggt
     6781 gaatcggctg ctcaagcagt tcgtcgagat gcagaagatg atgaagatgc tgtccggcgg
     6841 caagctgccg cgcacgggtg gaggactcgg cgccctgctg gggcgctgag gcaccgatgc
     6901 atcggctcgt catcgccgcg accacgctgc tggccctgac cggggcagtc gtcgtcggcg
     6961 cgtacctctt cctgttcgcc gcctcgagcg atcggctcgc cacgctggcg ccggagcgga
     7021 ccgcgttcta cgtgaacgtc tacctgcagc cctcgaccgg ccagcagatg aacctggccg
     7081 ggctcatcgg ccgcatcccc ggcttcgagg acacctccac cctcgacgag aagatcgacc
     7141 agatcgccga gaacctgctg tccgggtcgg ggatcgacta ccgcgaggac gtgaagccgt
     7201 ggctcggtaa ccagctcgcg atcgccgcct ggcccggcgg cgaccagctg gaggcgcagc
     7261 cggtgctgct ggctgaagtg aaggatcgcg aggcggccga ggcctccatc gccgacgtga
     7321 cgggcggcac ggatttccgg accgaggcct atggcggtgt ggacctccac gtgggcgacg
     7381 gcaccgcgta cgcgttcgtg gacgatcgaa ccctggtggt ggccacgcaa ccgacggacc
     7441 tccaggccat cgtcgacgtg gcgggcgggg aggccgacct cgcatcgcgc gcagacttcc
     7501 gcgacaccat ggcggatctg ccggccgacc acctggcctc ggtgttcgtg gacgtcgcgg
     7561 ccatcgcgga tgcgaccgac accgcgaccc agctctcgga ggtgagcacc gccggcgcgg
     7621 cgctggtcgc cgagcaggac ggcattcggc tgagcgggag cgcgccgttc gacatggcgg
     7681 acgcgggctc gtcgtccaag gcgacgttcg ccctcggcac ggagccgtcg agcctggtcg
     7741 actggatgcc ggacgacacg gtcgcggagc tggtcgtgtt cggcctgcgt cagacgctcg
     7801 aggacgccga ggcggcggca caggcgacgc cgcagggcca ggacgtgacc agcacgctcg
     7861 acgcggtccg cgccatcgcc gccttcggac tcggggtcga cctcgacagc gacatcctgc
     7921 ccctgctgga ccgtgaggtg ggcgtggcga tcaacggctt cgatggcgac ctgccgaccg
     7981 ggcagctgct gctccgcccg gatgatccgg acgcggccga gcaggccctc gaccgggtgg
     8041 cggacggcct cgtcaacctg gccggggcga cgcgcgacga cgtcgatgtg gatggcatca
     8101 gcgtgtcgac cctcgcggtc cccgacgtcc ccaccatcgc ctggacccgg gccgaggggt
     8161 tgctgatcct ggggctgcgc cccgaggacg tggcggccgc ggtgcgagcg catgagagcg
     8221 gcgaagccct cggcgccagc gaggcgtatc ggcgcacctt cgaggtcgcc ggcgcccgcg
     8281 ccggcaacga ggcctggatc gacgtgcagg ccgtcgttca gctcctgggg gccgatgcgg
     8341 acctgccgca ggacgctcgc gatatccttg ggcagatcgg ggccttcggc ttcacggctc
     8401 cgtcccgatc cgatcagatc gagttccacg cggtggtgac ggtcgacgac cgacccaccg
     8461 aatgaccgca gcacgacaac catcagagag gtgatcacca cttggcagtt cgcatccgat
     8521 tgacccgcgt cggcgccacg aagcgtcccg cgtaccgggt catcgccatc gaccagcggc
     8581 gctcgcggga cgggcgcgcc atcgagatcc tgggctacta cgatccgttg accgagccgg
     8641 cgacggtcca tctcgagacc gagaagatca aggcctggat cgggaagggc gcgcagccgt
     8701 ccgagacggt cagcaagctc atgcgcctgg ccgagaagcc ggccgccggg accgaggcgt
     8761 ccgacaagcc gaagcgcgcg acccgtgcgt cgaagccaaa ggcatcggcc aagaagacgg
     8821 aggacgctcc ggcggctgag cccgccgagg cgtcgacgga ctcggcggcg gaggcgtccg
     8881 ccgacgcgga gccgaccgag gcgtgaagga gttcctggag tacgtcgccc ggtcgctcgt
     8941 cgacaagccg gatgccgtct gggtcgaaga ggaccaggag ggcgacgaga ccatcctgac
     9001 cctgggcgtc gaccaggagg acatgggccg ggtcatcggc cgcgacggca ggatcgccaa
     9061 cgccatccgc agcctgctcc gcgtcatggc cacccgggat gggcgccacg tcgagctcga
     9121 gatcgtctcc gacgccgact gatcgcctgg ccgtcgcgcg catcatcggt ccgaagggcc
     9181 tggagggcgc cgtccggctc gagctcgtca ccgactggcc ggaggagcag atcgtcgtcg
     9241 gcgccgcgct gtggctcgac ggcgagacgg agccgatgcg cgtcacccgc gtcgagcgcg
     9301 gcggtcgcgt gctcgtcgtc cacctcgacg gtctggtctc gcgtgaggcg gccgaggcga
     9361 tggtcggccg ctacctggag gcgccggctc cgtcgctgcc cgctgacacc tacttctggc
     9421 acgacctgat cggcctccgc gtcgaggacg agcggggggc cgtcatcggt gagctggtcg
     9481 agatcttccg ggccgggggc aacgaggtct accgggtcat cggcccggac ggagagcggc
     9541 tggtcccggc gctgcgcagc gccatccgcg acatcgacct cgacggtggg cgcatggtgc
     9601 tggcacccga cgacgccgaa gaggtgcgct gaggacgccg atgcgcatcg acgtcgtcac
     9661 gctgttcccg gagctcatcg agccggcgct gcggacctcg atcatcggtc gcgccgccga
     9721 gagcggtcgc gtgcagttcg ggatccacga ccttcgggag catggcctcg gccggcaccg
     9781 ttccgtcgac gactacccct acggcggggg agcgggcatg gtcatgcgac ccgagccgct
     9841 gttcgcggcc gtcgagcccc tgcgcgccga gggagcgcac gtggtgctca tggacgcggc
     9901 cggtgagcgc ctgaccgatg cgctggcgcg cgagctcgcc gggattccgc acctggcctt
     9961 cgtctgcggt cggtacgagg gcgtcgacga gcgcgtcagg accctggtcg atcgcgaggt
    10021 gagcatcggc gactacgtgg tcaccggcgg ggagctgcca gccctggtgg cgatcgatgc
    10081 cgtcgtgcgg ctggtgccgg gggtcatcgc cgaggcgtcg cacgccgacg actccttcgc
    10141 atcgggcctg ctggagtacc cgcagtacac gcgaccggag gcgttccgcg agctggcggt
    10201 gccacccgtg cttctctccg gcaaccacgg cgaggtggag cgctggcgac ggcgggaggc
    10261 gctgcgccgc acgttggagc ggcgacctga cctcctgcac tcggtcgagc tgagcgccga
    10321 cgatcgtgcg ctgctcgagg agctggagcg agggcggccg gacgcctagc cgtgcttcgc
    10381 gggcgtggcc gcgcttgtcg cccgacgaag cgcgctgcta tactcccgcg gtcgcggccg
    10441 ctccacgcgg tcgcgttcgc atgccacgcc agtaagccgc gggtaagccc gcgataagag
    10501 gtcgccccca ggatgaacgc catcaaggag ctcgagcgcg agcagctccg gaccgacgta
    10561 ccggagatcg cccccggcga taccgttcgc gtctcggtca aggtcgtcga gggcaaccgt
    10621 gagcgcgttc aggtcttcga gggcaccgtc atgcggatgc gtggcggggg tctcaaccac
    10681 cagatcaccg ttcgccgtat cgccagcggc gtcggcgtcg agcgcacgtt catggtccac
    10741 gcgccccgca tcgacaagat cgaggtcgtc cgtcacggcg tcgcgcggcg cgcgcagctc
    10801 tacttcctgc gcgatcgcgt cggcaagtcc gccaccctcc gcgagcgccg ctcgaactga
    10861 cccggtgacc cggtccatct cggcaccggt ccaccgatcc cgtcccgtgc gcaccgcccg
    10921 tccccgcgcc cgcgacgcgc acatgcgtca cgagcgtgag ctgcacctgg ccggcttcca
    10981 ccgcgtcgcc ggcatcgacg aggtcggtcg cggttgcctg gcgggccccg tcgtcgccgc
    11041 ggccgtcatc ctcccggaac gccaccggat ccgcggtctg cgcgactcga aggtgctcgg
    11101 gcgcgctcgg cgcgaggcgc tctacgcgca gatcctggac cgtgccgagg ccgtcggcgt
    11161 cggctgcgtc gaggtcgaga cgatcgaccg aatcaacatc ctgcaggcca ccaagctggc
    11221 catgcgcgag gcactgggcc gcctgaacca gccggccgac cacctgctca tcgatgccct
    11281 gagcctccgc gacgtcgagc tgccccagcg gccgatcatc gacggcgacg ccatcagcgc
    11341 ctcgatcgct gccgccagca tcgtggccaa ggtgacgcgc gaccggatct gcgacgagat
    11401 ggatcgccgc ttcccggcct acggcttcgc caggaacaag ggttacggga cccgacacca
    11461 cgtcgatgcg ctgctgggcg aagggccgtg cgaatggcac cgtcgctcgt tcgcccccat
    11521 ccgccagctg ctggccgggg agcagctttc cctggagctc gacttcaccc tcgtcgacga
    11581 ggcgacgctc gaccccgact gacaccctcg cctcccgcga ggagatgcaa tggccgatcc
    11641 gcgccaccag ctcggccgtc gtgccgagga cgccgtggcc tcctggctga cccggtgcgg
    11701 ctggcgcgtc ctggccgttc gctaccggct gcggagcatc ggagaggtgg acctcatcgc
    11761 cctggatccc ggcgacgttc tggtggcgct cgaggtccgc gcgcgtcggt cgccgcgcac
    11821 gggcgcggcc gtggccaccg tcgactcccg aaggatcagc cgattggctc gcacgctggc
    11881 cgcgtacgcc gaggcgtctc ggacccggca ccgcggcctc cgcatcgacc tcgtggcggt
    11941 ggagccggac gcgacagccg ggtggcggat gatccgccac cccgccatcg gcgagcgttg
    12001 accttctccg ggcgcctcaa cccccgaaga gcacctcgag caggcggatc gaggcgatgt
    12061 acggtcgcgt gacgccgtag atgccgtgcc gatcggtggc gtactggccc tggtagtagg
    12121 cggccaggac cagatcgcgg tcgccctggt agcgctgcag gtagtgcgcg aggaggcgga
    12181 cgccggcgcg gacgttgtgg gtcgtgtcgt tgatgtcgac gggcgtgccc agcatggcct
    12241 cggccaccca tgcgcccgtc gcgggcatga gctgcatgac gccgacggcg cccgcggagc
    12301 tcacgacttc ctgccgccac ccggattcct gccaggcgac cgccagcgcg aaggccggtg
    12361 ggacgccgaa gcgctgcgcc tccgccacga tgagctgtcg catcgcgtca cgcccggcaa
    12421 ccagccgtgc catggagggt ggcatggcgg gcgcactcgg cgtcgatgcg gccgggggcg
    12481 tcgcagcggg agcggcggga gcgggtgccg gggctgtccc cggaatggtg agctgctgcc
    12541 caccgtagat ccggctcggg ttgctgatgc cattggccgc cacgatcgcc gagacgctga
    12601 cgccgtagcg acgcgcgatg gtccacaggc tctcgccgcg ggccacggta tggacggtcg
    12661 tggatggcgc ggtcgctgcc gctggcgcgg ccggagcagg cggtgccgcc gcagcgggag
    12721 ctgccggagc gggtgcggtc agacgcaggc gctgcccgac gtagatgcgg ttcggatccg
    12781 ccaggccgtt gagcgcgacg atgtccgaga tcgccacgcc ctgacgcttc gagatgccgc
    12841 tgagcgtgtc gccgcgctgg acgacgacga tcggatctgc ggccagcgcc acgccggcca
    12901 ggaacgggcc ggccgtcagc gtggcggcga ggccgagagc agggaagagg agccggggac
    12961 gctgggacaa catggacctc cggtgagcac ggccaggatc atcgaaattc gacgaaatct
    13021 cggttgcgtc ggtggagcga cgcgagccga tgctaggagc gatcggggcg gggtcaaggt
    13081 acgagcgggc tcccgcatcg gtactggagt accgtccggt gtggtccggt ccgcatcagt
    13141 gaccaaccgc gtgcgggaag agcgcaggcg cgctctggta tacttccgga cggcccacgc
    13201 gctggcgtgg tgccgcgtcc ccatcacaca cgcccggcgg atctcgctgg ccatcggtgc
    13261 tcgcgcctga acggcgcgag tcggcccgag acgcgaggcc gggcggagga acaaccggag
    13321 gtatctcttg gctgccacga ccatgaagca gctgctcgag gcgggcgtcc acttcgggca
    13381 ccagacccgg cgctggaacc cgaagatgcg cgagtacatc ttcgcggagc gcaacgggat
    13441 tcacatcatc gacctcgcgc agacggtcac gcgcctcgac caggcgctcg agttcgtccg
    13501 cgagacggtt cgccgcggcg agggcattct cttcgtcggc acgaagaagc aggcccagga
    13561 ggccatcgcc gaggcggcgc agcgctcgaa ccagcacttc gtgaacacgc gctggctggg
    13621 cggcatgctg acgaacttca acaccatcaa gcgccggatc cagcgactcg aggccctcga
    13681 ggcccgtcgc gactccggtg agttcgagcg cctgaccaag aaggaggcct cgaagctcaa
    13741 cgacgagatc acgaagctcg acgcggccct gggcggcatc cgcaagatgc gacgcctgcc
    13801 cggcgcggtc ttcatcgtcg acccgcaccg cgagtccatc gccgtcgcgg aggcccggcg
    13861 cctcgagatc cccatcgtcg cgatgaccga tacgaactgc gaccccgacg tcatcgactg
    13921 ggtcatcccg gccaacgacg acgcgatccg ctcggtcaag ctcgtgtgcg gcctggtggc
    13981 cgatgcggcc gccggcgcca tcgccgagcg gcaggcccgc ctggacgagg agttcgagca
    14041 ggccgaggcg atgccgccgg tcgacgaggc ggcgctcacc tcagaggccg actacaccgc
    14101 ggccctggcc tcgggcgagg cgctcgtctt cgagcccgag ccggaggacg acgaggagga
    14161 ggagcgtcgc gcccgcgcgc ggcgagccgc gcacctcgag gagggtgagc ccgagcgcga
    14221 tcccgaccgc gagcacgagc gcctcatcga cgagcacagc aaggagcccc gcgtctaggc
    14281 ggcggcagga aggaacatga cgaacaccct gatgatcaag cccgaggatg tgaagcggct
    14341 ccgggagcag accggcgccg gaatcatgga ctgcaagcgc gcgctcgagc agtcgggtgg
    14401 cgactacgag aaggccgcgc tgtggctgcg cgagaagggc ctgtccacgg cggccaagaa
    14461 ggccggccgt gcggcgcgcg agggcatcat cgccagctac atccaccacg gcgctcgcct
    14521 cggcgtgctg ctggagctca actgcgagac ggacttcgtc gctcgcaccg acgacttcca
    14581 gcagctgtcc cgggacatcg ccatgcagat cgccggcatg tcgccgctct acgtcagccg
    14641 cgatgacgtc ccggccgacg tcctcgacga gcagaagcgc ctcttcgcca tcgacgcgga
    14701 gcgcgacggc cggccagccg accgcatccc gatgatcgtg gagggcaagc tcaacaagtg
    14761 gttcgagcag gtctgcctgc tcgagcagcc gtatcgcgac acggacaaga agatgagcga
    14821 cctgatcacc gagaaggtgg ccctgctcgg cgagaacatc aaggtcgccc gcttcgcgcg
    14881 catggccgtc ggcgagacgg ccgatgcggc cgagccgacc gacgagggcg cctgatcccg
    14941 tgacggtgtc ggtcaccgcg gacgcgaagt cccgggagtc tcgccgggtg ctgctgaagc
    15001 tctccggcga ggccctcatg ggcgagcacg agtacgggat cgacccggcc gtggtgcgag
    15061 acatcgcctc tcaggtggcc gacagcgcgc gggaggggat ccaggtcgcg atcgtcgtgg
    15121 gaggtggcaa catcttccgc gggctggcgg cctccgcgcg cggcatggac cgcgcgaccg
    15181 gcgattacat gggcatgctg gcgacggtca tgaacggcct cgccatccag gacgcgctgg
    15241 agcaggtcgg ctgcccgacg cgggtcatga gcgcgatcgc catgaacgag atctgcgagc
    15301 cgtacatccg gcgtcgcgcg gtgcggcacc tcgagaaggg gcgcgtggtc gtcatggtcg
    15361 ccgggacggg caatccctac ttcacgaccg atacggctgc gacgctgcgc gccgtcgagg
    15421 tgcacgccca ggtgatcctc aaggccaccc gcgtcgacgg ggtgtacgat gccgatccgg
    15481 agacgcatcc cgaggcccag cgcttcagcc gcatcggcta cacgcagctg ctgagcagcc
    15541 ggctgcaggc gctcgatgcc accgccgtga gcctggcgat ggacaacgag atgccgatcg
    15601 tcgtcttcga catgacggtt ccgggcaaca tcgccagggc ggtccgcggc gagcggatcg
    15661 gtacgctgat ctcaggagag gagtcccgat gacgcacgag gcgctcaccg ccatcgaacc
    15721 caagatgcag cgtgccgtcg aagcgatgga gcgtgacttc gccggcttcc ggaccgggcg
    15781 ggcatcgacc gcgctggtcg agcggatcgt cgtcgattac tacggggcgc agaccccgct
    15841 gaaccagctg gccggcatca gcacccccga cccgcacctc atcgtgattc agccgtggga
    15901 ccgatcggtc ctgtcagcca tcgagaaggc gatcaccaag agcgacatcg gcctggtgcc
    15961 gaacgtcgac gggacggttg tccgcctcaa cgtcccgccg ctcaccgagg agcggcgccg
    16021 cgacatggtg aagcaggtcc gtcgccgaac cgaggaggcc aaggtcgagg tccgcaatca
    16081 ccgccgcgag gtcgccgacc agctgaagcg ctcgatgcgc gacggcgagc tgagcgagga
    16141 cgacgagcgc cgcgagctgg accagctgca gaagctcacg gaccgattca tcgaggccat
    16201 cgactcccgc gccgatcgca aggaagccga gatcctggag gtctgagcgt gccggaggcg
    16261 gtggctccac ggcacgtggc gatcatcgcc gacgggaacc gtcggtgggc gcgcgaccgc
    16321 gggcttccgg tcctcgcggg gcacgtgcag ggcatcgagg ccatccgtcc cgtcgttcga
    16381 cgcgcgcgcg accgcggggt ctcgacactg agcgtctaca ccttcagcac cgagaactgg
    16441 tcgcggcccg gggatgaggt caccggcctg ttcgggctca tcgacggcgc cgttcggcag
    16501 tacaccgacg agctgatcga ggagggcgtg cgagtccggg tcatcggccg cctgcacgag
    16561 gcgccggccg acgtccagcg ctccatccgc aacgccgagg agcgaacgca gggcggcacg
    16621 cggctcgacc tcaacatctg tttcaactac tcgggccggg cggagatcgt cgacgccgcg
    16681 cgcgagctca tcgccgcgcg cgtcgacccg gactcggtcg acgaggcgca cttcgccacg
    16741 cacctctaca cggcgggcca gcccgacgtg gacctgctga tccggaccgg tggggatgtc
    16801 cgaaccagca acttcctcat ctggcaggcg gcgtacgccg agctcgtctt ctgccccaag
    16861 ttctggccgg agttcacccc cgaggacttt gacgcggccc tcgacgactt cgcccgccgt
    16921 gagcggcgct tcggaggctg agccgatgct gcgcacccgc ctcctgaccg cggcaatcct
    16981 cggcccgatc attctcggcg tcgtcctgct cggcgagccg tggctgtcgc tgctcatcgg
    17041 cctcgtggcc ttcctggccc tggtggagat ggtctcgctg ctcgacgccg ccggctacca
    17101 gccgccccag gtgctcagca tcctggcggg catggcgctg accgcggcgg gcctgaccgc
    17161 cgcgaacgcc cagggcgtcg gcgggccggc cgccacgctg ctggcctcgc tcgacccgcc
    17221 tggcgtggtg gcctcgaccc tggtggcgac ggtgctgctc ctcgccgcgg ccgggttcat
    17281 gcgcggcgac ccgcgcgccg ggttcctgac ctgggcgatg accacgttcg gggtcgccta
    17341 cgtcggcctg ctgctgccga cgatcgtgct tgtggcgcac ctcgcgccgc cgggcgggag
    17401 ctggacgagc ggcgtcggcg ggctggggat cggctcgggc gtcgcctggg cgctgacctt
    17461 ggtgctcatc gtgtggggct acgacaccgg cgcctacctg acgggccgcc tggtcggccg
    17521 gaggcgactg gtcgagcaca tcagcccgtc gaagacggtc gagggggtcg ccgggggact
    17581 catcctgtcg accatcggtg ccgggatcgg cgcctggctg atcggcctgg agccgtggca
    17641 ccccctgatc atgggcccgc tggtcggcct cgccgcacag gccggtgacc tggccgagtc
    17701 gatgctcaag cgcgcggcgg gccgcaagga gtccgggttc ctggtacccg gccacggcgg
    17761 catcctcgac cgcgtggact ccttcctgtt cgccgcgccg atgctcgccg ggtacgccct
    17821 gctggtggcc ggccgggtgg tatgaccggc gtcgcgatcc tgggctccac cggctccatc
    17881 ggccggcagg cgctcgaggt gatcgaaggg ctgggcgacc ggttcgaggt gcgcgccctc
    17941 gcctccggtc gcggtggcgt cgggttcgcc cggcagctgg agcgctggcc tgccgccaag
    18001 gcgtggtgcg cgcaggacga ggcctccgtg cccgtatccc gtcgcgtcag cggcctcgcc
    18061 gagctcgcca cgctccacgg cgtggagctg gtcgtggtcg ccaccacggg gatgacggcg
    18121 ctgccagcgg tgctcgacgc gctgcgcagc ggacgccgcg tcgccctcgc caacaaggag
    18181 accctggtca ccggcggcca cctggtggca cgggagctgg acgagctggg cggcgacccg
    18241 ctggaccggc tgcggccgat cgacagcgag cactcggcga tctggcagtg cgtggtcggg
    18301 gaaccgcgtg aggcgatcga gcgtctggtg ctgaccgcca gcggcggccc ctttcgcggc
    18361 cgcccgcccg agtcgctggt ggacgtgacc gccgccgagg cgctcgcgca ccccacctgg
    18421 cgaatggggc cgaagatcac catcgactcg gccacgctcg tcaacaaggc gttcgaggtc
    18481 atcgaggcca aatggctgta ccggctcccg tactctagga ttgatgccgt gatccacccc
    18541 cagagcgtcg tccactcgct cgtcgagttc gcggacggct cgttcaaggc acagctcggc
    18601 ctccccgaca tgcgcctccc catccagtac gcgctgacct accccgagcg cacgccctcc
    18661 ccggcgcgcc gctcgcaccc cgccgactgg ggctcgctcg ccttcgcacc gctctcgccc
    18721 ggtgcctttc cggcgtacga cgtggtgcgg gcagcggccg ccgccggcgg gaaccgcggc
    18781 accatcgtca acgccgccga cgaagtcgcg gtcgccgcgt tcctggatgg ccgcatcggc
    18841 ttcacggcca tcgcacgcac catcgagcgc agcgtggagc gctggggcag cgatcgaacg
    18901 ccggatctcc acgacctcat cgccctggat ggcgaggtgc gcgacgcggt cgaggccgag
    18961 ctgcgctgat ggacgtgctc gccaccgtcg ccgccttcat cggcgtcctc gtcgtcctgg
    19021 tgctggtcca tgagctcggc cacttcgtcg cggccaagcg cagcgggatc accgtcgagg
    19081 agttcggcat cggctttccg ccgcgcatca cctcggtgac ctggcacggg acgcggtaca
    19141 gcctgaactg gatcccgctg ggcgggttcg tgaagatgct cggtgaggac ggtgacgtcg
    19201 agatccgcag gctgcgggag cgcgggctgg gtgacgccga gatcgagcgc gccatggacg
    19261 gcgccttcaa ccgacgcccc gtgtggatcc gcgtggcggt gctcctcgcc ggcgtggcca
    19321 tgaacttcgt ccttgcggcg gccctcttcg ccatcgcgtt cagcatgccg gtgccgttcg
    19381 gcaagggccc gctccaggtg gcctccatcc agcccgacag cccggcgagc acctcgctcg
    19441 aggtcggtga cgtgatcacc ggcgccgacg gggagacgtt cgatcgctcg gccgacctca
    19501 ccgactatat caacgagcgg gccggccgtc cggtcgtgct ccaggtccag cgcgacggaa
    19561 acgccgtgga cgtggccgtc acgccgcgcg tggtgagcga gcgggatcgc gccagcggca
    19621 tcggtcctgt cggcttcggc tacaagccgg cggaggtggg ggaggatccg ccgatcgccg
    19681 atggacccat cgacgcgatc gcccgcggga ccgggttcgc cggcaccctg gccgtgcaga
    19741 tccccggcgg cctggccgat gcggtcggcg gcctgctggg cctgaacccg aacgccggcg
    19801 acgcgcgcgg acccatcggt atcgcccagg agacgggcga agtcctcgat tcgccgctgt
    19861 tcaaccagct cctgttcgtc ggcctgctgt cggtcaacct ggccgtgctc aacgtgcttc
    19921 cgttcccgcc cctcgatggc ggccgcatcg cggtcgtgct ggtcgaggcc gtgcgccgtc
    19981 ggcggctgcc ggcggagcgt gaggcgctca tctacctcac cgggttcgcg gtgctcatcg
    20041 ccctggtcat cctgatcagc atccaggaca tccagcgcct catcaccggg ggctgacggc
    20101 cctcgcgcag ctgccg
//
LOCUS       DASTYP010000146        36048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_556990, whole genome shotgun sequence.
ACCESSION   DASTYP010000146 DASTYP010000000
VERSION     DASTYP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 36048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36048
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556990"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..363
                     /gene="rpsU"
                     /locus_tag="VFV59_06655"
     CDS             184..363
                     /gene="rpsU"
                     /locus_tag="VFV59_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU5325545.1"
                     /translation="MAEVRVGENESFESALRRFNKKIQQSGILAEARRREHYEKPSVK
                     RKRKEAKKRKASSRA"
     gene            424..897
                     /locus_tag="VFV59_06660"
     CDS             424..897
                     /locus_tag="VFV59_06660"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU5325546.1"
                     /translation="MTPEADTAMTLNQRIESAMRDAMRARDERRTQTLRMAMAAAHNA
                     RIARGRDLTDDEVVDVLTKQVKQRRESIEMYRAAGREDRAEAEEAEAAILGEFLPQQL
                     SDDEIESMARAAISESGASAPADMGRVMGRLSAQTRGRADGRRVSEIVRRLLAGG"
     gene            898..3009
                     /locus_tag="VFV59_06665"
     CDS             898..3009
                     /locus_tag="VFV59_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU5325547.1"
                     /translation="MFIPRTLAEQGRTGEAALGRALIIVTLVAAAIFLVLSANITVGQ
                     ENLEVGQVAPRDIRAPKDATFESDSLTEEARDAAAAAVTPSTDTVKSPADNLEDQLAL
                     FDPMARRVSRVLALRDNGTLEGAEVIQRIGSDAPEVPFTDRALVAELAPERWQAVVDG
                     ARAALAAALTDAVREDQLAEVRDSARSLVTTDLPADERDLAGDLAAEFIEPSEVIDVE
                     LTAQQRQQARDDTPPRMVTIRQGQTVVREGDPITVEVYEKLDELALTRPQVEPGTVLG
                     NALVSVLIASMLVAFLWRHEPEVWHRNRSLLLFFLAFVATAVVMRIAADRTLWAFVVP
                     TSGAVLLVGILLQGGAGAAMAIGLAVLAGVMNRDALEIAVFVLAGGLASLLAITRAER
                     LNVFVRVFGVLAATNLAVILAFGLLQQSNLAAILQLGVAGVANAFLSVILAVGSFHVL
                     GNLFGIMTVFQLLELANPSNRLLRRLLLETPGTYHHSVMVGNLAERAAETIGADPLLA
                     RVAAYYHDIGKMKNPLAFIENQAGAHNIHDDLSAETSARIIAGHIRDGIDLGYEHGLP
                     VQVIAFIPQHHGTSVMSYFHGKALREVSGREELVDERSFRYPGPKPQSREAAILMLAD
                     GVEASVRSLDEKDEESIRAMVDRIVDARVEDGQLDDAELTLKNIAQIKEAFVGQLLGM
                     YHSRIKYPDNVVPMEQRREHA"
     gene            3077..3664
                     /gene="ybeY"
                     /locus_tag="VFV59_06670"
     CDS             3077..3664
                     /gene="ybeY"
                     /locus_tag="VFV59_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5325548.1"
                     /translation="MACRVTVHDRIGADRLREAVEPTTPEDALALLESAVAETSRTAL
                     GAMETEVELTLCADPEMERLNFDHMGERGPTDVLSFPLHEWSVDGTHSRLAEEDGVSP
                     PGALLLGDVVIDVDQALRQAAQGRWGVVEEMALLAIHGTLHLLGHDHADLDEEERMRA
                     AERHVLGVLHRRHPHIEWQPGSLFDRAGHAVTTSS"
     gene            complement(3825..5063)
                     /locus_tag="VFV59_06675"
     CDS             complement(3825..5063)
                     /locus_tag="VFV59_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU5325549.1"
                     /translation="MGGIDTGDTAWVLMSTALVMLMTPGLALFYGGLVRRKNVLSTIM
                     HSFFTLGVVSVTWVLWGYSLAFGPDSGLGLVGRLDWLALEGVTATPSPTYAPTVPHLA
                     FMAFQMMFAIITPALISGSFAERKRFKAFVLFAVAWSTLVYAPVAHWVWSPDGWLFNL
                     GVLDFAGGTVVHLSSGVAALVAALFVGRRMGLVEPATEPHDVPMTVLGAGLLWFGWFG
                     FNAGSALGANGLAANALVTTNTAAGMAAITWVTVSWLHRGTPSVLGAALGAVAGLVAI
                     TPAAGFVTPAASVVIGLGVGLACYGAAQLRLRARVDDALDVFSVHGVGGAFGAIATGV
                     FATTAVNAAGADGALAGNLGQVAVQALGAGVVAAYSAIATAVILFAVNLVVSLRVPQH
                     EEEAGLDLAQHGEVAYQHAS"
     gene            complement(5186..6361)
                     /locus_tag="VFV59_06680"
     CDS             complement(5186..6361)
                     /locus_tag="VFV59_06680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5325550.1"
                     /translation="MEPHRTRSSEPPRSADVVVIGGGIIGCASAAMMADRGARVVLVE
                     GTAIGAGASGRNLGAIQHPFDPVLAPLYHETLSRYRALVASFDAGFAVPPAAAGLLLL
                     NADADAAARQAARMRADVPELDAHLLAPEDLADLEPALAPGLSAVRLETGFPIPPAAA
                     TDAWARLAVDRGVAIHVGRAARPSLQGGRVAGVAIDGAGVVATDRVLVAAGPWTPRLV
                     DPGGSWQPIRPTYGVTLQLRLGGPAPHHVVEEDEVDAINRAAAATARAAAADEAGVPS
                     IFSLASAGGISTLGSTFLPAEPNPTQVERLLLRRAVAFVPAVAEAELLGRRICARPQS
                     VDGRPFIGRVAADGLYVCAGHGPWGISTGPASAAIAARAVLDGTPPPVELAADRALG"
     gene            6399..6977
                     /gene="pth"
                     /locus_tag="VFV59_06685"
     CDS             6399..6977
                     /gene="pth"
                     /locus_tag="VFV59_06685"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5325551.1"
                     /translation="MKVICGLGNPGERFRFTRHNVGFRVVDLLADRWGLTGQGRVREG
                     AAVLEARLPEPVGRVLLVKPMKYMNLSGGPLRAALRQTEVELERELLVVADDIDLPLG
                     RVRLRRSGSAGGHNGLRDIIAALGTQEFGRLRIGVGRAGEAASHVLATFKPAERELAD
                     EMVQVGADAAERWLRDGIDEAMNAFNGVEIEP"
     gene            7029..10421
                     /gene="mfd"
                     /locus_tag="VFV59_06690"
     CDS             7029..10421
                     /gene="mfd"
                     /locus_tag="VFV59_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5325552.1"
                     /translation="MSPSRGGDRRVASLVDALRPHLPEPGVLRVPATARAAHLAARRA
                     ALPPHRPMVVVARTDEEAHRLADDLAAWVGATHVRTLPERAALPLERALPEHDESAER
                     LSVLAGLGGRSSNLVVVAPLLALAQRTLGAEQLQAARTSVVVGERVAQRTLLERLVAG
                     GYEPTVEVTGVGEFAHRGGLIDAWPPGRSEPVRIELFGDEIESIRTFDAMTQGSRRRH
                     TEVDLVPASEFIPVGGWEAAVASAPELGSDQLREDAARLTQGDLGESAETWAALLTAG
                     PAADHVTASTHLVLTDVDELRALAADLDEQAAERHAGLVRDEELPPEWPRPYDAMATL
                     DRLTRDAEERLEEQAEADAGYASAPPLPGRADRAGSWLVELAEAGRRVVVTTDQASRV
                     GELLEEVGHPTAPTSELRETPGSGGIAVVHGSLSSGFTHAPSELLLLTDRELFGATRV
                     RRLTAQKRVVTRDLIGKLQPGDHVVHVDHGIARYAGMTQRTFGEDVKEYLQLDFAGSD
                     KIFLPADQIGRITRYSGGPAPALSKLGGTEWERTKKRVRRAVGDLARELIEIYAARES
                     APGFAFSGDTTWQRELEESFPYTETPDQARSIEEVKADMLRRRPMDRLVVGDVGYGKT
                     EVALRAAFKAVQDGKQVAVLVPTTVLAQQHLLTFTRRLSAFPIEIAMLSRFVSKREQE
                     RIVDGVREGTVDILIGTHRILSKDIGFADLGLLVVDEEQRFGVSHKERIKQMRREVDV
                     LTLSATPIPRTLHLSLVGIRDLSVIETPPEARLPIQTRIAEDDDGLVRDAIARELDRG
                     GQVFYVHNRVETIEAAAERVRRLVPGARVGIGHGQMAEGMLERVMLDFSEGKFDVLVC
                     TTIIESGLDIPNANTIIIVRADTFGLAQLYQLRGRVGRSDRRAHAYLLHRRGMPLSPV
                     ARKRLHAIFSASDLGAGYQIALSDLEIRGAGNILGAEQHGFMAAVGFEMYTRLLAEAV
                     DMLRGRRPAPEPSPVRLDLPGSAYLPDDYIGDSGAKLEAYRRFAATRSEADAEALRAD
                     LRDRYGPIPAPVEGLFTAVRVRLAAEAAGVPDVRVEDRRVTLKWTRMPDRRAVSVALQ
                     VAGLRPDTASNQVRIPVVVGRDPIEVALRALDALGARGTAVS"
     gene            complement(10452..11216)
                     /locus_tag="VFV59_06695"
     CDS             complement(10452..11216)
                     /locus_tag="VFV59_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325553.1"
                     /translation="MTGFGPLLRKELLEQWRTKRLLVVIIVFIALGIGSAFLARYTAE
                     LIQALGGVPFEIELPEPTVADAVTQFLKNLGQAGILTAILLAMGSVATEKERGTAALL
                     LSKPASRGAYLASKLVAIATTLTAGMLLASIAGYVYTVLLFEPPSVTGWAGMTALLLL
                     SLFAYAALTFLGSTLTRSAVAAAAIGVAGMIVLAMVAAIPSIAPYLPAGISGQPAMAV
                     ALGTDPGPLLGPIAANVGLVLGLAALSWLAFRRQEL"
     gene            complement(11213..12178)
                     /locus_tag="VFV59_06700"
     CDS             complement(11213..12178)
                     /locus_tag="VFV59_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325554.1"
                     /translation="MRDGASAIRARGLSKRYGDVLALDGLDLDVPRGSVFGFLGPNGA
                     GKTTTLRILTSLATATDGSAEILGVPVGDGAPNRQGMIGYLDQDPRFHGWMRGRELLE
                     LVGRLYGLQGAALRGRIDEVLEVVGLAEAGRRTIGGYSGGMRQRLGLAQTILNRPPVL
                     LLDEPVSALDPEGRRDVLDVIRALGDGETTVLFSTHILNDVERVCDRVAILDRGRLVT
                     EAAMDELLDRYAGPVYEVEPDGLDDAGLARLAATLGDVGGVTGVERGHGVLRVSVDDG
                     PRASGRLLAALAAADLAVVRFERQRPTLEDVFLRLVGRNAGGAAS"
     gene            complement(12183..12383)
                     /locus_tag="VFV59_06705"
     CDS             complement(12183..12383)
                     /locus_tag="VFV59_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5325555.1"
                     /translation="MTLEEILPFLIPLIVLQLALMAVGLYDLTRPDRRVKGGNKLVWG
                     LVIVFGQMIGPLAYFLFGREDV"
     gene            complement(12720..13316)
                     /locus_tag="VFV59_06710"
     CDS             complement(12720..13316)
                     /locus_tag="VFV59_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325556.1"
                     /translation="MRILRTTAVPSLALALLLAACGGTGSTSSAADDDGGSQPSATAS
                     AAEDDGQGDGGGSAGDAGGDAAASVEVTITGGEFEGSYSGSVPDGGCSRGATGENTFG
                     LQYSTTEDVELSSVQLIVNDADAAASGSDDFQTTFTFGDLLNGTNVDLKPADGEGSGT
                     VTLDDRGDTATITIEGETSDGVGIDATVECHSVFDFGG"
     gene            13627..16464
                     /locus_tag="VFV59_06715"
     CDS             13627..16464
                     /locus_tag="VFV59_06715"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU5325557.1"
                     /translation="MTSHPTEPTGARSTLRVGDRDFAYYPLTAAGAGNLERLPYTVKV
                     LLENMLRGAATQPDLVSVDDVRALASWNPAQPAEAELPFMPARVILQDFTGVPCVVDL
                     AAMRDAMAELGGDPSRINPLVPADLVIDHSVQVDQFGTEAAFLINVEREYERNGERYA
                     LLRWAQQAFKDFRVVPPGTGIVHQVNLEFLADVVAERDGVLLPDTLVGTDSHTTMING
                     LGVVGWGVGGIEAEAALLGQPLYQPMPVVVGFRLDGELPSGATATDLVLFVTEMLRRH
                     GVVGKFVEFYGPGLSKLPLADRATISNMSPEFGATATLFPVDDETLRYLRMTGRPDDV
                     IARVEAYTRAQGLFRTDDTPEPIFSESLSLDLSTVVPSLAGPKRPQDRVALTELRDGF
                     RAAFPDGLEDATEPPLGQVEEASEESFPASDAPSYTREAAEAEPAAPMPARASPPAPG
                     TPAQRAYRSVPVELEGERFDLRSGSVVIAAITSCTNTSNPSVMVAAGLLARNAVERGL
                     TTKPWVKTSLAPGSRAVTDYLENAGLMAPLEALRFNLVGYGCTTCIGNSGPLAEPIAE
                     AIEGNDLVGVAVLSGNRNFEGRIHPLARASYLASPPLVVAFALAGRIDVDLTTEPIGT
                     DRDGRPVMLADLWPSPKEVASVVLKSVSGDVFRRNYASVFEGDERWTTLPVPAGDRYA
                     WDPASTYVARPPFFEGLGGELPPPQDITGARVLAVLGDSVTTDHISPAGSIARTSPAG
                     RWLMEHGVEPRDFNSYGSRRGHHEVMMRGTFANIRLRNLLTPDAEGNVTEHLPSGERM
                     SIFDAAMRYAEDGTPLVILAGKEYGSGSSRDWAAKGPMLQGVRAVIAESYERIHRSNL
                     VGMGILPLQFLPGENATSLGLTGRETFDVLGVADGLLPRQELTVVARGEDGREVRFRV
                     RSRLDGEIDVTYLRNGGVLQTVLRRLAGLSA"
     gene            16568..17098
                     /locus_tag="VFV59_06720"
     CDS             16568..17098
                     /locus_tag="VFV59_06720"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU5325558.1"
                     /translation="MTVSSVDDLRALIREVPDFPKPGILFYDITTLLREPDAFREVID
                     RMADAIGDTKVDVVVGMESRGFIFSAPLAYKLGAGFVPVRKLGKLPAETIEVEYELEY
                     GTATLEIHADAIDKGQRVLIVDDLLATGGTVMGTIELVRRLGGEIAGLSFMVELTALH
                     GREKLDDFDIHALLSY"
     gene            17122..17985
                     /locus_tag="VFV59_06725"
     CDS             17122..17985
                     /locus_tag="VFV59_06725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325559.1"
                     /translation="MAVASSHRLQARAVSDRSEIAAFLRRDRLYAAYALGDLDGPNRA
                     RVSWALASDEHGEPTSLVMHHEGLVPQPLFLMGAPDGCRSILESVIRPRDAYLQATEI
                     HEPALRDLYDLDTPVEMLRMVVDRGAFAPFAGPAERLTALDIEDLNRLYQLGFRAGFP
                     PSVLDEGIYYGVRVRGRLVSAAGTHALNRHEGIAVVGNVMTHVDFRGHDFAKMVTSAV
                     TAALLDVVPDVTLNVHADNDPAVAAYSRLGYRTHCRLVERLVHRRASGGWGLMRPIRE
                     AMRIPWQREPK"
     gene            17982..19247
                     /gene="ahcY"
                     /locus_tag="VFV59_06730"
     CDS             17982..19247
                     /gene="ahcY"
                     /locus_tag="VFV59_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5325560.1"
                     /translation="MTLKTADVADLALADAGRLKIEWADRQMPVLAGIRERFEHERPL
                     EGWKISACLHVTAETANLMRTLAAGGADAVLCSSNPLSTQDDVAASLVTHFEIPTFAI
                     KGEDRDSYYAHIRATLDHHPEITMDDGADLVNELHANRPEQLPEVLGGTEETTTGVIR
                     LRAMANAGKLAYPVVAVNEALTKHLFDNRYGTGQSAIDGILRATNILFAGKTVVVGGY
                     GWVGRGIASRAHGMGANVVVCEVDPVRALEAAMDGYRVMNAMDAAHVGDVFISGTGNL
                     NVWGREHFPVMPDGAILANAGHFNDEFELPALEEQSRSSREVRPFTREYLMPDGRRLY
                     VLADGRLVNLGAAEGHPAMVMDMSFANQALGLEWLRTNVDGLEPKVYGIPEDIDREVA
                     RLKLESMGIRIDPMTAEQKAYSESYDSGT"
     gene            19348..19908
                     /locus_tag="VFV59_06735"
     CDS             19348..19908
                     /locus_tag="VFV59_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5325561.1"
                     /translation="MGMARGETLGDRGAGGARGEPVAEQAISAHGAYEEHADALRAFL
                     FATVRDASEAEDLLHEAFLRLLTETAGGRAPDHPRAWLFRVAMNLAASRARRRGVAAR
                     RAPELVQHDVAPSPEEQLLAREAAHAVHLRLAGLPDHARVALLLAAHGFSGAEIARRI
                     GRSELATRSLLCRHRGRLRSTSPEAA"
     gene            19952..22945
                     /locus_tag="VFV59_06740"
     CDS             19952..22945
                     /locus_tag="VFV59_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5325562.1"
                     /translation="MPSVSSPVFVGREAELVWLDRAFELASTGEPTTRLIGGEAGIGK
                     TRLVTEFVGRLRRRDAEILIGDCLQLGDTGLPYAPFVAALRPLLRSLPRERLDAIIGP
                     GRDALAHLLPDLGGRAAPSAPDASFASTARQARLFEVVFGVLRRLADERPVVLVLEDL
                     HWADSSTRDLLRFLVRNARDGRLLFIATYRSDELHRRHALRPFLAEVQRVAGVEVREL
                     DAFDAGEIAAQVTAITGRPARDEVVSALLARSGGNPFFAEELIVGGEAGLALPRSLRD
                     TLEDRVRQASADAQRLLRVAAVAGDRVQHGLLAQVLDLPADRLHEALREVIEHHLLVP
                     TRPDEVPGYRFRHALLQEVVYEALLPSERTQLHAAYAAVLEADPQLALGHASGAGAQI
                     AHHWVLAHDLERALPAMLRAAHEATASYAFPEAKAFLERALELWPQIAPERLPGDVDR
                     VTILSEAAEAAAHAGDARRSIDLVRAALAETDPLGEPMRAGVLHHRLAWYLNESGDWQ
                     SGVAALDRAVALIPIDPPTPERARVLSDLAHSLMVRGRYGDSLAIAEAALSIARSVEA
                     RVAEARALNALGLDLACRSDFERGLPILREGFDLAVEMGDPLAIFLSAAGYGWALDES
                     ARHAEALELAREASERIRLLGAEARFGGQLASKVARALYDLGRWTEALHIIDDTLAAS
                     PTRYAVRWLLSNRIRILVHRGELEAAKRDIATYDALGERVIGPDPDLVKRRRAELSIV
                     AGTPTAARGLVREVLDQLVEPELDADARPLLLVGLRAEAVEADLARAGGSAERRRAAA
                     RRADELLTSMRAHVQRVTTSAWNPAPVIDADRVLAEALASRARGETEVELWQDAVAGR
                     RRLARPHELAAALGDLAAVALESGHRDVGAAALVESHGIAVALAARPLREQLEALARR
                     ASIQLPGVPSADDAAGKLGLTRREREVLALLADGRSNRQIGEALFMAESTAGVHVSNI
                     LGKLGVSRRTEAAALAHRLGLFGVL"
     gene            22992..24146
                     /locus_tag="VFV59_06745"
     CDS             22992..24146
                     /locus_tag="VFV59_06745"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEU5325563.1"
                     /translation="MLDASTRRTGGSLLAILAGLALLTAVALLPDLTPSDALPVVGQS
                     SAQAVIVQALPPDATGAARFAVELRSGDDAGTTIEAIVQDGSAALPGSATREPYEVGD
                     EVVVTQFAGEAGGFAVISDPWRVPLLGLVAALFAAVVLVVGGLRGARSLVALALTFAV
                     IVKLVVPLLLRGVDPVLLAVVGGIAITVATLTLTEGFGRVTLAAVLGTSAALLLTAVL
                     AAAFTAAAAFSALQGSEEIAFLIPLTGDRLDLSGILLAATVFGALGVLDDVTVTQAAT
                     VDELHRSDPAAGRGAVIARAMRVGRSHIAATVNTLMLAYLGVGLPLLLLFALAGQDPL
                     TVLNGEIVAVEVIRALVGSIGIVAAVPLTTAVAALLGVPATPAARSDASA"
     gene            24191..25387
                     /gene="metK"
                     /locus_tag="VFV59_06750"
     CDS             24191..25387
                     /gene="metK"
                     /locus_tag="VFV59_06750"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5325564.1"
                     /translation="MTDRVPPMLFTSESVTEGHPDKLCDAVADAILDDVLAHDPDARS
                     AVEVATTTGLVLVMGEVSTTHYVDVQAVVRRTVREIGYNSSHLGFDWETCGTMTAIKE
                     QSPDIALGVDRALEARSGEGPEAEGPGAGDQGMMFGYASDESPELMPMPIMLAHRVAR
                     RLAEVRKDGTLPFLRPDGKSQVTVEYDADHRPRRVHTVLVSTQHDPDVDLADLSAAVR
                     RHVVEAAIPGELLDDATRPLVNPTGRFVIGGPMADSGLSGRKIIVDTYGGMGRHGGGS
                     FSGKDPTKVDRSGAYMARYVAKNVVAAGLARRCELQLAYAIGVARPLAINVQTFGTGR
                     LPDTRLAQVVEQVFDLRPGAIIRDLDLRRPIYRPTAAYGHFGRDDLDLPWERTDRGDD
                     LRAAAG"
     gene            25508..26098
                     /locus_tag="VFV59_06755"
     CDS             25508..26098
                     /locus_tag="VFV59_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325565.1"
                     /translation="MRGLVGMDASPSARPGIGELPAVLRRDPAAPSYLAAERRGDHES
                     AAAAASEASERAIADEAWWPADAWAHRALWHYEQAQLTLEAARQARRIGDLRTAAGDP
                     ESARRYYAEAIDEARDIGAEHEQGLAALGLGRAMLELGQVTQARRLASAAIDLLERST
                     ASTLELDAAHALLGREQVVTPGAGAPATRDEATEES"
     gene            26098..26940
                     /gene="proC"
                     /locus_tag="VFV59_06760"
     CDS             26098..26940
                     /gene="proC"
                     /locus_tag="VFV59_06760"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU5325566.1"
                     /translation="MARRTMRVGIVGAGVMAEAIIAGLVADRAVTPDLLVASHPRRER
                     RESLSERHGIRAVAANRDALGEAEVVVLAVKPQMLGRVMRELRGRLDAEQVVVSIVAG
                     ATLRTLVDGLQHASVVRAMPNTPSQIRRGMSVWAASDACTGHQRDLARAVLRALGDER
                     QVADETFVAMATALSGTGPTYLFAVMEALIDAGVHLGFPRELAHDLVVETLVGSAEYA
                     KRSELHPAQLRNAVTSPGGTSAAAIYELEKGRIRTVFSDAVWAAYRRTVELGERLEAE
                     VERD"
     gene            26940..28010
                     /locus_tag="VFV59_06765"
     CDS             26940..28010
                     /locus_tag="VFV59_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5325567.1"
                     /translation="MFAIVMAGGSGTRLWPLSRRRNPKQLLNLTGDTSLLQQTVARLG
                     TLLRPHDIYVITSQAHVRATQEQLPQLPADNVLGEPLARSTAVAAGLATVLARRESDE
                     VALVLPADHYVADEDAFVAGLREAARAAERGYLVTLGVVPTHAATGYGYIRAGAAFHP
                     SSRTAPVEAFVEKPDADRARDFLADGSYLWNAGIFVWRVYAFRQAVERFQPRLAEALD
                     RVGALHRTPGWMSEVREILEPIPAITIDTGIAEPAAAEGRMAVVPLEAGWSDIGSWSA
                     LLEALSHARGADLVASGRHLDRGSHNVLVHGGDRLVVTVGLEDVIIVDTPDALLVCSR
                     ERAEEIKPVLDEIARTAGDQYL"
     gene            28055..28654
                     /locus_tag="VFV59_06770"
     CDS             28055..28654
                     /locus_tag="VFV59_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325568.1"
                     /translation="MAAYVRLVAATCRIDGPPVSDEQVVLAFWHEYNLAAAVAAHRLR
                     RRHHHVSFSTRTFRGRVMNSMLAGLDSGAVPLPDETQRSESARLARELARLGREGSSL
                     AVSPDGPFGPYRRAKPGALIVARHAGLPLQPWAVVARPPLRLRRRWDRHLVPLPFGRL
                     RVVEGESLRVGPRDALRPLVARFQQALDSTSARADAAES"
     gene            28715..30160
                     /locus_tag="VFV59_06775"
     CDS             28715..30160
                     /locus_tag="VFV59_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEU5325569.1"
                     /translation="MTKIKFGTDGWRAAIAEDYTFHNVRRCARGVAEHLQQTGAADRG
                     VVVCHDRRFASEHFARACVEVLAAHGIRSFTPPEAVPTQVGSFFTRELNAGAGIVITA
                     SHNPWTDNGFKVKADTGGAAAPEMLAAIEATMDTHPEDELPPRRDHDDAVEAGLVEIF
                     DPYPKFRDQLASIVDIEKIRAAEKRVLVETLYGSGSGWYTRLLGGGRLTVRELHTERN
                     PYFGGVNPEPIRPNVDEWLEEIPRWGADIGIAFDGDADRVGMATEEGVFVNQLQVYGL
                     LYWYLLDVRNQIGPAVYTVTTTSMAKRLAEIYGTRAYETGVGFKYVGPKMTETDAVIG
                     GEESGGFGFGMHIPERDGLASGLFLLDLWLTKGKRASEVLAELQALAGPSYYNRIDIR
                     FAREGYDQVKTDTLARLAEHAPTELGGLEVVERKDLETGDGFKYYRSDGSWLLIRFSG
                     TEALLRVYVEARSPEDVDALLAEGRRIAGAR"
     gene            30160..31356
                     /locus_tag="VFV59_06780"
     CDS             30160..31356
                     /locus_tag="VFV59_06780"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEU5325570.1"
                     /translation="MDARDFFGYKHADEEPGRRPETDDGTEEDRVPTFEEAVTLDDPE
                     TLRRIDPQDMLGKIMAMPAQLAQARRVAAPIRLEQRHRDVDAIVVLAMGGSAIGAELV
                     AAAAGERLRVPLIVHRDYGLPAGVGDRTLVIAASHSGETAETLSGFAAARARGVPLAV
                     ITTGGRLAAAATEAGVPLLQYRLGGQPRAAIGFGVGLVHELLSGADLIVDGDPLGPVV
                     QALEELLERIGPGVETSANPAKQLAWAAFGRIPVVYGHGSMAAVAHRWKTQLNENAKA
                     WAAWEPMPEANHNAIEGSLNPRELSDALYVVQLRDADEPEEIGQRYRVVEELLGERAT
                     NRSQVRAEGPSALARVLTAVAYGDMFSAYLAILYQTDPTPVTLLAMLKERLARATGSD
                     PTGADS"
     gene            31393..31686
                     /locus_tag="VFV59_06785"
     CDS             31393..31686
                     /locus_tag="VFV59_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325571.1"
                     /translation="MQSSMIGKVEKAMRYAHEPDRVKFASFRASFAGDNGTHTVTLDA
                     DSWHCDCHLFEAAGGCTHTLAIQKMLDPMLTDAARETPLYAHADIRDEEAVPA"
     gene            31756..33195
                     /locus_tag="VFV59_06790"
     CDS             31756..33195
                     /locus_tag="VFV59_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325572.1"
                     /translation="MTHQLLAVLGSIAHGIRSFIGAILGLLGLARFARFAVPLLVAAL
                     AVAAVVSARDTAVILGSRPTVTDATLPDVASHTDEVGGSVWYAFDALIAQQSFPSPTE
                     AGTFFYLALDPEDPSQGLLVRSPLNDAFFRQRVLSADIVEDSDTVATALDAFGTLPAG
                     FEVDRVRLLDETSAGGDLADAFVPSQLADEASGENLLVTGRVVSPATFGVCGEPDGCQ
                     AAGARYLYLFADAEGGHAFVLRSPHPPAAIPVRLEGLYLRDTFDLAPVVESDWFRSIE
                     AEVPTGRAFQANTRPPITVEASWVPTIVFAALALLLLASHLAGYPVFGRARRQPEPGH
                     SLGVGEGVDVAITGRMARDRATLQLDRSPGALERLPIPELALRMWRYGLLSPDLSRQE
                     AERRFVEEAGASGNRLVVHERDQSALVVLGRGSGAAVQAGRLHRLGRSTPAVHVRDDR
                     TDAHLELRSAAERDRVAAEILAEVGESAD"
     regulatory      33232..33375
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            33403..34389
                     /locus_tag="VFV59_06795"
     CDS             33403..34389
                     /locus_tag="VFV59_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5325573.1"
                     /translation="MAPRTRQRRVTLLAAALVALLATACSTPSGTAAPSTDPTASDLA
                     SPGSQMTVTDARGAEVTIDGVPERIVSLAPSNTEIVCALNACDRLVGVTDFDDYPPEV
                     ADVADVVVSAQVDVERVVDAQPDLVLAAGNELTPTSVITQLEGLGLTVIVLYPESLDE
                     VTDDIELLGGVLDEADAAASLVAGMRQRVGAITAAVEGLDRPRTLYEVYYAEGVTYTA
                     GEGSFLASLIELAGGEPVTGDAQGTLPAEQLVAADPQLVLLGTASYDPSLATPAAALE
                     AVRARPGWSELSAVRDAEVLPYLDDIVTTRPGPRIVDGLEALARAIHPEAFE"
     gene            34404..35480
                     /locus_tag="VFV59_06800"
     CDS             34404..35480
                     /locus_tag="VFV59_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU5325574.1"
                     /translation="MRATTATRPLVLGPTLGRSSVVLGLGAVLLVAAAAVALAAGTSG
                     VAPGDALAIVAHRLLGLGSVSWPASAETIVWELRLPRIVAGMVVGAGLASSGVVFQAL
                     LRNPMADPYLIGTAAGASLGAVAALLLPSLLPIAATAWLGIGAVQVLAFAGGLATVLL
                     VFAVARGRMGTPVVTLLLAGYAVSSLLSAGVALLMFVAGDRLAAVVSWLLGSLGGASW
                     PRLALAAPLIGISFVLLLVRWRALNALLLGEGHAATLGIDVEREKRRLTALAALATSA
                     GVAISGTIGFVGLVVPHLLRLAFGPDHRLLLPASTIYGAVLLVLADTGARLAGGIPVG
                     IVTALIGAPFFIWLLRRSLAVPRG"
     gene            35473..>36048
                     /locus_tag="VFV59_06805"
     CDS             35473..>36048
                     /locus_tag="VFV59_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325575.1"
                     /translation="MADLLPVLELTDVRAGYGRRRVLDGASLSVAAGEVVAVLGTNGS
                     GKSTLLRVAAGLLRPWSGSVRILGRPSGAWPRRDLARRVAVLPQALELPLGFRVAEVV
                     AMGRIPHARALFADDGDDGSAVAAALRDADATDLADREVSELSGGERQRVLVAMALAQ
                     EPDLLLLDEPTLHLDLAHQLTLVQTLARLRRG"
BASE COUNT         4931 a        13127 c        13095 g         4895 t
ORIGIN      
        1 gacgtctcca gcctgtcgcg cggtgaccat cgcaacggtg gcccgcgtgc gttgaccctc
       61 cgcacctccg ctgctagact ccgcgggctt gtgcgccggg ttggcgtacg gcagtcgcat
      121 gcctgcccat ccgtccagcc cacacccacg gcgcaacacc gaagcagaga ggaggcgaga
      181 cgtttggcag aggtccgcgt gggcgagaat gaatcgttcg agagcgcgct gcgccgcttc
      241 aacaagaaga tccagcagtc gggaatcctg gccgaggcgc ggcgccgcga gcactacgag
      301 aagcccagcg tgaagcgcaa gcgcaaggaa gccaagaagc gaaaggcctc ctcgcgcgcc
      361 tagccgccag acgccagcct caacccgaca acgcatccgg cagcggagga ccaacgcaga
      421 ccgatgacgc cggaagccga caccgccatg accctgaacc agcggatcga gtccgccatg
      481 cgtgacgcca tgcgcgcgcg cgacgagcgg cgcacgcaga cgctgcgcat ggccatggcc
      541 gccgcgcaca acgcgcgcat cgcgcgcggc cgagacctca ccgacgacga ggtcgtggac
      601 gtcctcacca agcaggtgaa gcagcgccgc gagagcatcg agatgtaccg cgccgcggga
      661 cgcgaggacc gagccgaggc cgaagaggcg gaggccgcga tcctcggcga gttcctgcct
      721 cagcagctgt ccgacgacga gatcgagtcg atggcgcgcg ccgccatcag cgaatcgggg
      781 gcgagcgcgc cggccgacat gggccgcgtc atgggtcgcc tctcagcgca gacccgcggc
      841 cgtgcggacg gtcgccgcgt gagcgagatc gtgcggcggc tcctggcggg cggctaggtg
      901 ttcatcccgc gcaccctggc ggaacagggg cggaccgggg aggcggcgct cggccgcgcc
      961 ctgatcatcg tcacgctggt cgcggccgcg atcttcctgg tcctctccgc gaacatcacc
     1021 gtcgggcagg agaacctgga ggtcgggcag gtggcgccgc gcgacatccg cgcgccgaag
     1081 gacgcgacat tcgagtccga cagcctgacg gaggaggcgc gcgacgcggc cgcggcggcc
     1141 gtcacgccat caaccgacac ggtcaagtcg ccggccgaca acctcgagga ccagctggcg
     1201 ctgtttgacc cgatggcgcg gcgcgtgagc cgggtgctcg ccctgcgcga caacgggacc
     1261 ctcgagggcg ccgaggtcat ccagcgcatc ggctcggacg ccccggaggt cccattcacc
     1321 gaccgtgccc tcgtggccga gctggccccg gagcgctggc aggcggtcgt cgacggggcg
     1381 cgcgccgcac tcgccgccgc cctcaccgat gccgtgcgcg aggaccagct ggccgaggtc
     1441 cgcgactcgg cccgcagcct ggtcaccacc gacctgccgg ccgacgagcg agacctggcc
     1501 ggcgacctgg ccgccgagtt catcgagccg tcggaggtca tcgacgtcga gctcaccgcg
     1561 cagcagcgtc agcaggcccg cgacgacacg ccgccgcgca tggtgacgat ccggcagggc
     1621 cagacggtcg tgcgcgaggg cgacccgatc acggtcgagg tctacgagaa gctggacgag
     1681 ctggcgctca cgcggccgca ggtggagccc gggaccgtgc tgggcaacgc gctggtatcg
     1741 gtcctgatcg cgtcgatgct ggtcgccttc ctgtggcgcc acgaacccga ggtctggcac
     1801 cgcaatcgat cgctgctgct cttcttcctg gcgttcgtgg cgaccgccgt cgtcatgcgc
     1861 atcgccgcgg accggacgct gtgggccttc gtggtgccga cgtcgggcgc ggtgctgctg
     1921 gtcggcatcc tgctccaggg cggcgccgga gcggcgatgg cgatcggcct ggcggtcctg
     1981 gccggcgtca tgaaccggga cgcgctcgag atcgcggtct tcgtgctggc cggcggcctg
     2041 gcgtcgctcc tcgccatcac ccgcgcggag cggctgaacg tcttcgtgcg ggtcttcggc
     2101 gtgctggccg cgacgaacct cgccgtgatc cttgccttcg gcctgctcca gcagtcgaac
     2161 ctcgcggcca tcctgcagct gggcgttgcc ggcgtggcga acgccttcct gtccgtcatc
     2221 ctggccgtcg gcagcttcca cgtgctgggc aacctgttcg ggatcatgac ggtattccag
     2281 ctgctggagc tggcgaaccc gtcgaaccgg ctgctgcgcc gcctgctgct cgagacgccg
     2341 gggacgtacc accacagcgt catggtcggc aacctggccg agcgcgccgc cgagacgatc
     2401 ggggcggacc cgctgctcgc gcgcgtggcc gcctactacc acgacatcgg caagatgaag
     2461 aacccgctgg ccttcatcga gaaccaggcg ggggcgcaca acatccacga cgacctgagc
     2521 gcggagacga gcgcccggat catcgcgggg cacatccgcg acgggatcga cctgggctac
     2581 gagcacggcc tgccggtcca ggtcatcgcg ttcatccccc agcatcacgg caccagcgtg
     2641 atgagctact tccacggcaa ggcgttgcgc gaggtcagcg gacgcgagga gctggtggac
     2701 gagcgcagct tccgctaccc cggtccgaag ccgcagagtc gcgaggcggc catcctgatg
     2761 ctcgccgacg gcgtcgaggc cagcgtccgc tcgctggacg agaaggatga ggagagcatc
     2821 cgcgccatgg tcgaccgcat cgtcgatgcg cgcgtcgagg acgggcagct cgacgacgcc
     2881 gagctgacgc tgaagaatat cgcccagatc aaggaggcct tcgtcggtca gctgctgggc
     2941 atgtaccact cccgcatcaa gtaccccgac aacgtggtgc ccatggagca gcgccgggag
     3001 cacgcctgag ggccggtccg gaccgcggcg cgcgaccttg agcgcgccga tccgggggga
     3061 ctacgatggt cgcgacatgg cctgcagggt caccgtgcac gaccgcatcg gcgccgatcg
     3121 tctccgcgag gcggtcgagc cgacgacgcc cgaggatgcg ctggccctgc tcgagtcggc
     3181 cgtcgccgag acgtcgcgaa cggcgctcgg cgccatggag accgaggtcg agctgacgct
     3241 gtgcgccgac ccggagatgg agcgtctgaa cttcgaccac atgggcgagc gcgggccgac
     3301 tgacgtgctc agcttcccgc ttcacgagtg gagcgtcgac ggcacccact cacggctggc
     3361 ggaggaggac ggcgtctcgc caccgggggc gctcctgctc ggggacgtgg tgatcgacgt
     3421 ggaccaggcg ctgcgccagg cggcgcaggg gcggtggggc gtggtcgagg agatggcgct
     3481 gctcgccatc cacggcacgc tgcacctgct ggggcatgac cacgccgatc tcgacgagga
     3541 ggagcggatg cgcgcggccg agcgccacgt gctgggggtg ctccaccggc gccacccgca
     3601 catcgagtgg cagccgggct cgctcttcga ccgggccgga cacgcggtga cgaccagcag
     3661 ctgaaccggt cgagtcctcg ggagacgcgt gccgctcagg cgtcgcggtg acggtgcgtt
     3721 ccgtccggcg cgacgaccca cgcgagcgcg acgagcgcga agctgacgag cgcggcggtg
     3781 accatcggtt cctgacctcc tatcggttcg aggattgagc gtgctcagga cgcgtgctgg
     3841 taggcgacct cgccgtgctg agcgaggtcc aggccggcct cctcctcgtg ctgcggcaca
     3901 cggaggctga cgaccaggtt gacggcgaag aggatcacgg cggtcgcgat ggccgagtac
     3961 gccgcgacga cgccggcgcc cagcgcctgg accgcgacct ggcccaggtt tccggccagc
     4021 gccccgtcgg cgccggcggc gttcacggcg gtcgtggcga agaccccggt cgcgatggcc
     4081 ccgaaggcgc cgccgacccc gtggacgctg aacacgtcga gcgcgtcgtc gacccgcgcg
     4141 cgcaggcgca gctgcgccgc gccgtagcac gccaggccga cgcccaggcc gatcacgacc
     4201 gacgcggccg gcgtcacgaa ccccgcggcc ggcgtgatgg ccaccagccc ggccacggcg
     4261 ccgagcgcgg cgccgaggac tgacggcgtt ccgcggtgca gccagctgac ggtgacccag
     4321 gtgatggccg ccatgcccgc ggcggtgttc gtcgtgacca gcgcgttggc ggccagcccg
     4381 ttggcgccga gcgccgagcc ggcgttgaag ccgaaccagc cgaaccacag caggcccgcg
     4441 ccgaggacgg tcatcggcac gtcgtgcggc tcggtggccg gctcgaccag ccccatccgt
     4501 cgcccgacga agagggcggc caccagcgcc gccacgccgc tggacagatg caccaccgta
     4561 ccgccggcga agtccaggac gccgaggttg aacagccacc cgtccgggga ccacacccag
     4621 tgggcgactg gcgcgtagac gagcgtggac caggctacgg cgaagaggac gaaggccttg
     4681 aaccgcttgc gctcggcgaa gctgccgctg atgagggcgg gggtgatgat ggcgaacatc
     4741 atctgaaagg ccatgaacgc caggtgcggc acggtcggcg cgtaggtggg ggacggcgtg
     4801 gcggtgacgc cttcgagggc gagccaatcc agccgaccga cgaggccgag cccgctgtcc
     4861 ggaccgaagg ccaggctgta gccccacagc acccacgtga cgctcacgac gccgagcgtg
     4921 aagaagctgt gcatgatcgt gctcaggacg ttcttgcgcc ggaccaggcc gccgtagaag
     4981 agcgccagtc cgggcgtcat gagcatgacc agcgccgtgc tcatcaggac ccacgccgtg
     5041 tcgccagtgt cgataccgcc caaggtggcc tcccgctgtg caccgctttg tgcacggcag
     5101 cctaggcacc gatgcgaccg tgggacgcct gaagttcgag gtccgtcggc gcgatcattg
     5161 tgcaggatgc acaatacggg gcggttcagc cgagggcgcg atccgccgcg agctccacgg
     5221 gtggtggcgt cccgtcgagg acagcccggg ccgcgatggc ggccgatgcc ggaccggtgc
     5281 tgatgcccca gggtccatga ccggcgcaca cgtacaggcc atcggcagcc acgcggccga
     5341 tgaacggacg accgtcgacg gactggggcc gggcgcagat ccgccgcccc agcagctcgg
     5401 cctccgccac ggccggcacg aatgccaccg cgcgacgcag gagcaggcgc tcgacctgcg
     5461 tcggattcgg ttccgcggga aggaaggtgg acccgagcgt gctgatgccg cccgcactgg
     5521 cgaggctgaa gatggaggga acgcccgcct cgtccgccgc tgcggcgcgg gccgtggcgg
     5581 cagcggcgcg gttgatcgcg tcgacctcgt cctcctcgac gacgtggtgg ggcgcggggc
     5641 cgccgaggcg gagctggagc gtcaccccgt acgtcgggcg gatcggctgc cagctgccac
     5701 ccggatcgac gaggcgcggc gtccacggtc cggcggcgac caggacgcgg tccgtcgcga
     5761 cgacgccggc gccgtcgatc gcgacaccgg cgacccgtcc gccctgcagc gatggccggg
     5821 cggcgcgccc cacgtggatg gccacgccgc gatcgacggc gagccgcgcc catgcatcgg
     5881 tggcggcagc cggcgggatc gggaagcccg tttccagccg gacggccgac aggccgggag
     5941 cgagcgccgg ctcgaggtcg gccaggtcct cgggagccag caggtgggcg tcgagctcgg
     6001 ggacgtcggc gcgcatgcga gcggcctggc gagcagccgc atcggcgtcg gcgttgagga
     6061 ggagcaggcc cgcggctgcc ggcgggacgg cgaatccggc gtcgaacgac gcgaccaggg
     6121 cgcggtagcg cgacagggtc tcgtggtaca gcggcgcgag caccgggtcg aaggggtgct
     6181 ggatcgcgcc gaggttgcgg cccgacgcgc ccgcgccgat cgccgtgccc tccaccagca
     6241 cgacccgcgc gccacggtcc gccatcatgg ccgcgctcgc gcagccgatg atcccgccgc
     6301 cgatgacgac gacgtcggcg ctgcgcggcg gctcggagga tcgggttcgg tgcggctcca
     6361 cggccgcgag gatagtcggc cgggtagcat cggcgccgat gaaggtgatc tgcgggctcg
     6421 gcaaccccgg cgagcgcttc cggttcacgc ggcacaacgt cggctttcgc gtggtcgacc
     6481 tgctggccga ccgctggggc ctgaccgggc agggtcgggt gcgcgaggga gcggccgtcc
     6541 tcgaggcgcg cctgccggag ccggtcggcc gggtgctgct ggtcaagccg atgaagtaca
     6601 tgaacctgtc cggcggtcca ttgcgtgccg ctctgcggca gaccgaggtg gagctcgagc
     6661 gcgagctgct ggtggtcgcc gacgacatcg atctgccgct gggtcgcgtg cgcctgcgac
     6721 gctccggctc ggccggcggc cacaacggcc tgcgcgacat catcgcggcc ctcgggacgc
     6781 aggagttcgg acggctgcgc atcggcgtcg ggcgcgccgg cgaagcggcg agtcacgtgc
     6841 tcgccacctt caagcccgcc gaacgcgagc tggccgacga gatggtccag gtcggagccg
     6901 acgccgccga gcggtggctg cgggacggga tcgacgaggc gatgaacgcc ttcaacggcg
     6961 tggagatcga gccctagcgg cggcgatcgg gtccggtcgg gcccgacgcc ctaccatgga
     7021 ccgcctccat gtcgccatca cgcggcggtg atcgccgcgt cgcttcgctc gtcgacgccc
     7081 tccggccgca tctgccggaa ccgggcgtcc tccgcgtgcc cgcgaccgcg cgggccgcgc
     7141 acctggcggc gcgccgggcg gcgctgccac cgcatcggcc gatggtcgtc gtggcccgca
     7201 ccgatgagga ggcgcaccgg ctggccgacg acctggccgc gtgggtcggc gccacccacg
     7261 ttcgaacgct cccggagcgc gcggccctgc cgctcgagcg tgcgctgccg gagcacgacg
     7321 agtcggccga gcggctctcc gtgctcgccg gcctgggcgg tcggtcgagc aacctcgtcg
     7381 tcgtcgcgcc gctgctggcg ctggcgcagc ggaccctcgg ggcggagcag ctgcaggcgg
     7441 cgcggacgtc cgtggtcgtc ggcgagcggg ttgcccagcg gacgctgctc gagcggctgg
     7501 tcgccggcgg ctacgagccg acggtcgagg tcaccggggt gggggagttc gcgcaccgtg
     7561 gtggtctgat cgacgcctgg ccgcccggcc gctccgagcc ggtgcggatc gagctgttcg
     7621 gtgacgagat cgagtcgatc cggaccttcg acgccatgac ccagggctcg cgacgccggc
     7681 acacggaggt cgacctcgtc cccgcctcgg agttcatccc cgtcggcggt tgggaggcgg
     7741 ccgtggcatc cgcgcccgag ctcgggtccg accagctgcg cgaggacgcc gccaggttga
     7801 cgcagggcga cctgggcgag tctgccgaga cgtgggccgc cctgctgacc gcgggcccgg
     7861 ccgccgatca cgtcaccgcc tcgacgcacc tggtcctcac cgacgtcgac gagctgcggg
     7921 cgctggcagc cgacctcgac gagcaggccg cggaacgaca cgccggcctg gtgcgcgacg
     7981 aggagctgcc cccggaatgg ccccgcccgt acgacgccat ggcgacgctg gaccggctga
     8041 cccgggacgc ggaggagcgg ctggaggagc aggcggaggc cgatgccggc tacgccagcg
     8101 cgccgccgct ccccgggcgt gccgaccgcg ccgggtcgtg gctcgtggag ctggcggagg
     8161 ccggccgccg ggtggtcgtc accacggacc aggcgagccg cgtcggcgag ctcctggagg
     8221 aggttggaca tccgacggcc ccgacgtccg agctgcgcga aacgccgggg tcgggcggga
     8281 tcgcggtggt ccacggctcg ctctcgtccg ggttcacgca cgcgccatcc gagctgctgc
     8341 tcctcaccga tcgcgagctg ttcggcgcga cgcgggtgcg tcgcctgacc gcccagaagc
     8401 gggtcgtcac gcgggacctc atcggcaagc tccagcccgg cgaccacgtc gtgcacgtgg
     8461 accacggcat cgcgcgctac gccgggatga cgcagcgcac gttcggcgag gacgtgaagg
     8521 agtacctcca gctcgacttc gccggcagcg acaagatctt cctccccgct gatcagatcg
     8581 gccggatcac gcgctactcg ggcggccccg cgcccgcact cagcaagctg ggcgggaccg
     8641 agtgggagcg aacgaagaag cgcgtgcgtc gcgcggtcgg cgacctggcg cgcgagctga
     8701 tcgagatcta cgcggcccgc gaatcggcgc ccggcttcgc gttcagcggt gacacgacct
     8761 ggcagcgcga gctggaggag agcttcccgt acaccgagac gccggaccag gcgcgcagca
     8821 tcgaggaggt caaggccgac atgctgcgcc gccgcccgat ggaccgcctg gtggtcggcg
     8881 acgtcggcta cggcaagacc gaggtggcgc tgcgggcagc cttcaaggcg gtgcaggacg
     8941 gcaagcaggt ggccgtgctc gtgcccacca cggtcctggc gcagcagcac ctgctcacct
     9001 tcacgcgtcg cctgtccgcg tttccgatcg agatcgccat gctcagcagg ttcgtgtcga
     9061 agcgggagca ggagcgcatc gtcgatggcg tgcgcgaggg cacggtggac atcctgatcg
     9121 ggacgcatcg gatcctctcg aaggacatcg gcttcgccga tctcggcctc ctggtggtgg
     9181 acgaggagca gcgcttcggg gtcagccaca aggagcgcat caagcagatg cgacgcgagg
     9241 tcgacgtcct gacgctgtcc gcgacgccga tcccgcgcac actgcatctc tcactcgtcg
     9301 gcatccgtga cctgtccgtg atcgagacgc cgccggaggc gcggctgccg atccagactc
     9361 gcatcgcgga ggacgacgac ggcctggtgc gcgacgcgat cgcgcgcgag ctggaccgtg
     9421 gcggccaggt gttctacgtc cacaaccggg tcgagacgat cgaggcggcc gcggagcggg
     9481 tgcggcggtt ggtgcccggg gcgcgcgtcg gcatcggcca tggccagatg gccgagggca
     9541 tgctggagcg agtgatgctc gacttcagcg aggggaagtt cgacgtcctg gtgtgcacca
     9601 ccatcatcga gtccggcctc gacatcccga acgcgaacac catcatcatc gtccgcgccg
     9661 ataccttcgg gctggcgcag ctgtaccagc tgcgcggacg ggtgggccga tcggaccgcc
     9721 gcgcccacgc ctacctgctg caccgtcgtg gcatgccgct ctcgccggtg gcgcggaagc
     9781 ggctgcacgc catcttttcg gcgtcggacc tcggcgccgg ctaccagatc gccctgtcgg
     9841 acctcgagat acgcggcgcc ggcaacatcc tgggcgccga gcagcacggc ttcatggcgg
     9901 cggtcgggtt cgagatgtac acgcgactgc tggcggaggc ggtcgacatg ctgcgcggcc
     9961 ggcgcccggc gccggagccc tcgccggtca ggctggacct gccgggatca gcgtacctgc
    10021 ccgacgacta catcggcgac tcgggggcca agctcgaggc ctaccggcgc ttcgccgcga
    10081 cgcgatccga ggcggacgcc gaagccctgc gtgccgacct gcgcgaccgc tacgggccga
    10141 tccccgcgcc cgtggagggc ctgttcaccg ccgtgcgcgt tcgcctggcc gccgaggccg
    10201 cgggcgtccc cgacgtgcgc gtcgaggatc ggcgggtgac gctgaagtgg acgcgcatgc
    10261 ccgatcggcg tgcggtcagc gtggcgctgc aggtcgccgg cctccgcccg gatacggcgt
    10321 cgaaccaggt tcggatcccg gtggtcgtgg ggcgcgaccc gatcgaggtc gccctgcgcg
    10381 cgctcgacgc gctgggggcg agggggaccg ccgtcagcta ggggaggcgg agctcccagg
    10441 tgatcgtcat ctcagagctc ctggcgccgg aaggcgagcc agctcagcgc cgccagtccg
    10501 agcaccagcc cgacgttcgc agcgatcggc ccgagcagcg gaccgggatc ggtgccgagc
    10561 gcgacggcca tggcgggctg gccgctgatt ccggcgggca ggtagggcgc gatgctcggg
    10621 atcgccgcca ccatggccag cacgatcatc ccggcgacac cgatcgccgc ggcagcgacg
    10681 gcggagcgcg tgagcgtgct gcccaggaac gtgagtgccg cgtaggcgaa cagcgagagc
    10741 agcagcagcg cggtcatacc ggcccagccc gtgacgctcg gcggctcgaa cagcagcacc
    10801 gtgtagacgt agccggcgat cgaggccagc agcatgccgg ccgtgagggt cgtggcgatg
    10861 gccacgagct tgctggccag gtacgcgccg cgcgacgccg gcttggacag cagcagcgcc
    10921 gcggtcccgc gctccttctc cgtcgccacc gaccccatcg ccagcaggat cgcggtgagg
    10981 atccccgcct ggccgaggtt cttgaggaac tgggtgaccg cgtcggcgac ggtcggctcg
    11041 ggcagctcga tctcgaacgg cacgccgccc agtgcctgga tgagctccgc ggtgtagcgc
    11101 gccaggaacg ccgagccgat gccgagcgcg atgaacacga tgatcaccac cagcagccgc
    11161 ttggtgcgcc actgctccag cagctccttg cgcagcaacg gtccgaatcc ggtcatgacg
    11221 ccgctcctcc ggcgttgcgg ccgacgagcc gcaggaagac gtcctccagc gtcggccgct
    11281 gtcgctcgaa gcgcacgacc gcgaggtcgg cggccgcgag tgccgccagc agccggccgg
    11341 aggcccgggg gccgtcgtcg accgatacgc gcagcacgcc gtgtcctcgc tcgactccgg
    11401 tgacgccgcc gacgtccccg agcgttgcgg cgaggcgcgc gagacccgcg tcgtcgagtc
    11461 cgtccggctc cacctcgtac accggcccgg cgtagcggtc gagcagctcg tccatcgcgg
    11521 cctcggtcac caggcggccg cggtcgagga tcgcgacgcg atcgcagacg cgctccacgt
    11581 cattgaggat gtgggtcgag aagaggaccg tcgtctcccc gtcgccgagg gctcggatga
    11641 cgtcgaggac gtcccgccgg ccctcggggt cgagggcgct caccggctcg tccaggagca
    11701 gcaccggggg acggttcagg atggtctggg ccagcccgag ccgctgccgc atgcctcccg
    11761 aatagccgcc gatggtgcgc cggccggctt cggccaggcc gacgacctcg aggacctcgt
    11821 cgatccggcc gcgcagcgcg gccccctgca ggccgtacag ccggcccacc agctcgagca
    11881 gctcgcggcc gcgcatccag ccgtggaagc gtggatcctg gtccaggtag ccgatcatgc
    11941 cctgccggtt gggcgcaccg tcgccgacgg ggacccccag gatctcggcg ctgccatcgg
    12001 tcgcggtcgc cagcgaggtc aggatccgca gggtggtggt cttgccggcg ccgttggggc
    12061 cgaggaagcc gaagacgctc ccgcgcggga catcgaggtc cagcccgtcc agcgccagca
    12121 cgtcgccgta tcgcttggac aggccgcgcg cgcggatggc ggaggctccg tcgcgcatcg
    12181 gctcagacgt cctcgcggcc gaagaggaag tacgcgagcg gaccgatcat ctggccgaac
    12241 acgatgacca gtccccacac cagcttgttg ccgcccttga cgcggcgatc gggccgcgtc
    12301 aggtcgtaca ggccgacggc catgagcgcc agctggagca cgatcagcgg gatcaggaag
    12361 ggcaggatct cctcgagggt catgagcggg ccacctccga gccacggcgc gccgggtcgt
    12421 cgcgccccac caggtacgag ttcgccgcca gcacgacctt catgacgcct ccttcgcgcc
    12481 gatccccggc gtggacatcg ggaccgccat cgcctcctcg agcaccttca gggtggggtg
    12541 gcaggtgatc gacgtcccgt gccgatcggc ctgcacgaga tcggtcgaac gtcgctcgcg
    12601 ccgtttgccg ggcctcgttc ggtgtggacc gaagcatgcc accggtggcg agggggtcga
    12661 ggcgacgaag ggcgcgacgc ttgcgcgccg cgccccgtga ccggaccgag tggcgggcgt
    12721 cagccgccga agtcgaacac cgagtggcac tcgaccgtcg cgtcgatgcc gacgccgtcg
    12781 ctggtctcgc cctcgatcgt gatcgtggcc gtatcgccgc ggtcgtccag ggtcaccgtg
    12841 cccgagccct cgccgtccgc gggcttcagg tcgacgttcg tcccgttgag caggtcgccg
    12901 aacgtgaagg tcgtctggaa gtcgtcgctg ccggacgcgg ccgcatcggc gtcgttgacg
    12961 atcagctgga cgctgctgag ctcgacgtcc tcggtggtgc tgtactgcag gccgaaggtg
    13021 ttctcgcccg tggccccgcg gctgcagccg ccatccggca cgctgccgga gtaggagcct
    13081 tcgaactcgc caccggtgat ggtgacctcg acactggccg ccgcgtcccc gccggcgtcg
    13141 ccggccgacc cgcctccatc gccctggccg tcgtcttcgg ccgcgctcgc cgtggcgctg
    13201 ggctggctgc cgccgtcgtc gtcggcagcg ctcgacgtgg agccggtccc gccgcacgcg
    13261 gcgaggagga gcgcgagcgc gaggctcggc acggcggtgg tgcgaagaat ccgcatgagc
    13321 agtggtgccc tcccgtggtg gtcacagcgc tgccggacaa ggccttcgcg tcagcgaacg
    13381 cacgccggca tcggctcacc gggcagcgtc ggcgaggggc ggcggtgcga gagggagtcc
    13441 cccgtggcga gcggatgatg cccggcctcc acggattccg caatcggtca tgcgacgtac
    13501 tcgatggtcc tccgccgtcg tacggtagac tggtctgacc actcgacccg cgacgggcgc
    13561 acccgtccgg cgccgatctt cgaccgatga cgcgtcccgc ggacgcgacg acgaaaggag
    13621 gcgaccgtga cgtcccatcc caccgagccc accggcgctc gttccacgct gcgcgtcggc
    13681 gaccgcgact tcgcgtacta cccgctgacc gccgccggag cgggcaacct cgagcggctt
    13741 ccctacaccg tcaaggtgct gctggagaac atgctccgcg gcgcggccac acagccggac
    13801 ctcgtgagcg tcgacgacgt ccgggcgctg gcctcctgga acccggcgca gccggccgag
    13861 gccgagctgc cgttcatgcc ggcccgcgtc atcctgcagg acttcaccgg cgtcccgtgc
    13921 gtcgtggacc tcgccgccat gcgcgatgcc atggcggagc tgggtggcga cccgtcgcgc
    13981 atcaacccgc tggtgcccgc cgacctggtg atcgaccaca gcgtgcaggt cgaccagttc
    14041 ggcacggagg cggcgttcct catcaacgtc gagcgcgagt acgagcgcaa cggcgagcgg
    14101 tacgcgctcc tgcgctgggc gcagcaggct ttcaaggact tccgcgtcgt cccgcccggc
    14161 acgggcatcg tgcaccaggt caacctggag ttcctggccg acgtggtcgc tgagcgcgac
    14221 ggcgtgctgc tgcccgacac tctcgtcggg accgactcgc acacgacgat gatcaacggc
    14281 ctgggcgtcg tcggctgggg ggtcggcggg atcgaggcgg aggcggcgct gctcggccag
    14341 ccgctctacc agccgatgcc cgtcgtcgtc ggcttccggc tcgacggtga gctgccctcg
    14401 ggggccacgg ccacggacct ggtcctgttc gtgaccgaga tgctgcgccg ccacggcgtg
    14461 gtgggcaagt tcgtcgagtt ctacgggccc ggcctgagca agctgccgct cgcggaccgc
    14521 gccacgatct cgaacatgtc gccggagttc ggcgccaccg cgaccctctt ccccgtcgac
    14581 gacgagacgc tgcgctacct gcgcatgacc ggccgacccg acgacgtgat cgcccgggtc
    14641 gaggcgtaca ccagggccca gggcctgttc cggaccgacg acacgccgga gccgatcttc
    14701 agcgagtcgc tgtcgctcga cctgtcgacg gtcgtgccgt cgctggccgg gccgaagcgg
    14761 ccgcaggacc gggtggcgct gacggagctg cgggacggct tccgcgcggc cttccccgac
    14821 ggcctggagg atgcgaccga gccgccgctc ggccaggtgg aggaggcgag cgaggagtcc
    14881 ttcccggcct ccgacgcacc gtcctacacg cgcgaagcgg cagaggctga gcccgcagcg
    14941 ccgatgccgg cgcgggcatc gccgccggcg ccggggacgc ccgcgcagcg ggcgtatcgg
    15001 tccgtgcccg tggagctgga gggcgagcgc ttcgacctgc gctccggctc ggtggtgatc
    15061 gcggcgatca cctcgtgcac caacaccagc aacccctcgg tcatggtcgc ggccggcctg
    15121 ctggcgcgca acgcggtcga gcgcgggctg accaccaagc cgtgggtcaa gacctctctg
    15181 gccccgggat cgcgcgccgt caccgactac ctcgagaacg cgggcctgat ggctccgctc
    15241 gaggcgctgc gcttcaacct ggtcggctac ggctgcacga cctgcatcgg caactccggc
    15301 ccgctggcgg agccgatcgc ggaggcgatc gaaggcaacg acctggtggg cgtggctgtg
    15361 ctgtccggca accgcaactt cgaggggcgc atccacccgc tggcccgggc gagctacctg
    15421 gcctcgccgc cgctggtcgt ggccttcgcg ctggccgggc ggatcgacgt cgacctcacg
    15481 accgagccga tcggcaccga ccgcgacgga cggccggtga tgctcgccga cctctggccc
    15541 tcgccgaagg aggtcgcgtc ggtcgtgctg aagtccgtca gcggtgacgt cttccgtcgc
    15601 aactacgcct cggtgttcga gggcgacgag cgctggacga cgctcccggt cccggccggc
    15661 gaccgctacg cctgggatcc cgcctcgacc tacgtggcgc ggccgccgtt cttcgagggc
    15721 ctcggcgggg agcttccgcc gccgcaggac atcaccggcg cccgcgtcct ggccgtcctg
    15781 ggcgacagcg tcaccacgga tcacatctcg ccggccggct ccatcgcccg cacctcgccg
    15841 gccgggaggt ggctgatgga gcacggcgtc gagccgcgcg acttcaactc gtacgggtcg
    15901 cggcgcggcc accacgaggt catgatgcgc ggcacgttcg ccaacatccg gctgcgcaac
    15961 ctgctcacgc cggacgcgga gggcaacgtc accgagcacc tgcccagtgg ggaacggatg
    16021 agcatcttcg acgccgccat gcgttacgcc gaggacggga cgccgctcgt catcctggcc
    16081 gggaaggagt acggatccgg ctcgtcgcgc gattgggccg ccaagggacc gatgctccag
    16141 ggcgtgcgtg ccgtcatcgc cgagtcctac gagcgcatcc accgttcgaa cctggtcggt
    16201 atgggcatcc tgccgctgca attcctgccc ggcgagaacg ccacctcgct gggcctgacg
    16261 ggacgcgaga ccttcgacgt gctgggcgtc gccgacggcc tgctgccccg gcaggagctg
    16321 accgtcgtgg cgcgcggcga ggacgggcgc gaggtccgct tccgcgttcg gtcgcggctc
    16381 gacggtgaga tcgacgtcac ctacctgcgc aacggcggcg tgctgcagac cgtgctgcgc
    16441 cggctggcgg gcctcagcgc ctgacgcacc acgggcggcc ggcgcgttgc cccggccgcc
    16501 cgcatcggtc tagactcacc gggcgccgtt cgcccctgac cgccgcacca aggagtgtcc
    16561 ggataccgtg accgtctcgt ccgtcgatga cctccgcgcc ctgatccgcg aggtcccgga
    16621 cttccccaag ccggggatcc tcttctacga catcaccacc ctgctccgcg agcccgacgc
    16681 gttccgggag gtgatcgacc gcatggccga tgcgatcggc gacacgaagg tcgacgtcgt
    16741 cgtcggcatg gagtcccgcg ggttcatctt cagcgcgccg ctcgcctaca agctgggcgc
    16801 cgggttcgtc ccggtccgca agctcgggaa gctgccggcc gagaccatcg aggtcgagta
    16861 cgagctcgag tacgggacgg cgacgctcga gatccacgcc gatgcgatcg acaagggcca
    16921 gcgcgtgctg atcgtcgacg acctgctggc cacgggcggc acggtgatgg gcaccatcga
    16981 gctcgtgcgc cggctcggcg gcgagatcgc cggcctctcg ttcatggtcg agctcaccgc
    17041 gctgcacggt cgcgagaagc tcgacgactt cgacatccac gctctgctga gctactagtc
    17101 ggcgggggga gcaccgacgt catggcggtc gcgtcctccc accggctcca ggcccgcgcg
    17161 gtcagcgacc ggtcggagat cgccgccttc ctacgtcgcg atcggctcta cgctgcctac
    17221 gccctgggcg acctcgacgg cccgaaccgc gcccgcgtca gctgggcgct cgccagcgac
    17281 gagcacgggg agccgacctc gctggtcatg caccacgagg gcctggtgcc gcagccgctg
    17341 ttcctgatgg gggcgccgga cgggtgccgc tccatcctcg aatcggtgat ccgcccgcgc
    17401 gacgcgtacc tgcaggcgac cgagatccac gagccggcgc tccgcgatct gtacgacctg
    17461 gacacgccgg tcgagatgct gcgcatggtg gtcgaccgtg gggccttcgc gccgttcgcc
    17521 ggcccggccg agcggctcac cgccctggac atcgaggatc tcaaccggct ctaccagctg
    17581 gggttccggg cgggcttccc gccatcggtc ctcgacgagg gcatctacta cggcgtccgc
    17641 gtccgcgggc ggctggtgag cgcggccggc acgcacgcgc tgaaccgcca cgagggcatc
    17701 gcggtcgtcg gcaacgtcat gacccacgtc gacttccgcg gtcatgactt cgccaagatg
    17761 gtgacgagcg ccgtgacggc cgcgctgctc gacgtcgtgc ccgacgtgac gctcaacgtc
    17821 cacgccgaca acgaccccgc cgtcgcggcc tactcgcgcc tcggctaccg tacgcactgc
    17881 cgcctcgtcg agcgcctcgt ccaccggcgg gcgtccggcg gctggggctt gatgcgaccg
    17941 atacgagagg cgatgcgaat tccgtggcaa cgcgaaccga aatgacgctg aagacggccg
    18001 acgtggccga cctggccctg gccgatgccg ggcgcctgaa gatcgagtgg gcggatcgcc
    18061 agatgccggt cctggccggc atccgcgagc ggttcgagca cgaacggccg ctcgagggct
    18121 ggaagatcag cgcctgcctg cacgtgacgg cggagacggc gaacctgatg cgcacgctgg
    18181 ccgcgggcgg cgccgatgcg gtgctgtgct cttcgaaccc gctgtcgacc caggacgacg
    18241 tggcggcgtc gctggtgacc cacttcgaga tcccgacctt cgccatcaag ggcgaggacc
    18301 gggattcgta ctacgcgcac atccgggcga cactcgacca ccacccggag atcaccatgg
    18361 acgacggtgc ggacctggtc aacgagctcc acgccaaccg gcccgagcag ctgcccgagg
    18421 tcctgggcgg caccgaggaa acgacgaccg gtgtcatccg gctgcgggcc atggcgaacg
    18481 ccggcaagct cgcctacccg gtggtcgcgg tgaacgaggc gctcaccaag cacctgttcg
    18541 acaaccgcta cgggaccggc cagagcgcga tcgacggcat cctgcgcgcg acgaacatcc
    18601 tcttcgccgg caagacggtc gtggtcggcg gctacggctg ggtggggcgg ggcatcgcct
    18661 cgcgggccca cggcatggga gcgaacgtgg tcgtctgcga ggtcgatccg gtccgtgccc
    18721 tggaggccgc catggacggc taccgcgtca tgaacgcgat ggacgcggcc cacgtggggg
    18781 acgtgttcat cagcggcacc ggcaacctga acgtctgggg ccgggagcac ttcccggtga
    18841 tgccggacgg ggcgatcctg gccaacgcgg gccactttaa cgacgagttc gagttgccgg
    18901 ccctcgagga gcagtcgcgg tcgtcgcgcg aggtgcgtcc cttcacgcgc gagtacctga
    18961 tgcccgacgg gcggcgcctc tacgtcctgg ccgacggccg gctggtcaac ctcggcgcgg
    19021 ccgaggggca ccccgccatg gtcatggaca tgagcttcgc gaaccaggcg ctcggactcg
    19081 agtggctgcg gaccaacgtc gacggcctgg aaccgaaggt gtacggcatc cccgaggaca
    19141 tcgaccgcga ggtggcccgc ctgaagctgg agagcatggg catccggatc gacccgatga
    19201 cggccgagca gaaggcctac agcgagtcct acgactccgg cacctgaccc tccgtacgtc
    19261 gcgctccggc ggccgggtcg agaaatagtc gaaaaaataa aggttctcca gatgcgcgtc
    19321 gccgaccttt agggctaagg tccctgcatg gggatggccc ggggggagac gctcggtgac
    19381 cggggcgcgg ggggcgcccg gggggagccg gtcgccgagc aggccatttc cgcccacggc
    19441 gcgtacgagg agcacgctga cgccctccgc gccttcctgt tcgccaccgt gcgggatgcg
    19501 tccgaggcgg aggatctgct ccacgaggcg ttcctgcgcc tcctgaccga gaccgccggt
    19561 ggccgcgcgc ccgaccatcc ccgcgcgtgg ctcttccgcg tcgccatgaa cctcgcggcc
    19621 agccgcgccc gccggcgagg cgtcgccgcg cgccgagcgc cggagctggt ccagcacgac
    19681 gtggccccgt cgccggagga gcagctgctg gcccgggagg cggcccatgc cgtgcacctg
    19741 cggctggccg ggctgcccga tcacgcgagg gtggcgttgc tgctcgccgc ccacgggttc
    19801 tcgggcgccg agatcgcccg ccgcatcggt cgctccgagc tcgccactcg ctctctcctg
    19861 tgccgccatc gagggcggtt gcggagcacc agcccagaag cggcctgacc ggccgtccat
    19921 cgcggggcgt ctcgtggcta tcatcgagcc catgcccagc gtcagcagcc cggtcttcgt
    19981 cggccgcgaa gcggagctgg tgtggctcga ccgcgccttc gagctcgcgt ccaccggtga
    20041 accgaccacg cgactgatcg gcggtgaggc ggggatcggg aagacccgcc tggtcaccga
    20101 gttcgtcgga cggctgcgga ggcgtgacgc cgagatcctc atcggtgact gcctgcagct
    20161 cggggacact gggctcccgt acgcgccgtt cgtggccgcc ctgcgacccc tgctgcgctc
    20221 gctgcctcgg gagcgactcg acgccatcat cggccccggc cgcgatgccc tcgcccacct
    20281 cctgccggac ctcggcggcc gtgccgcgcc gtcggcgccc gacgcctcgt tcgcctcgac
    20341 cgcccggcag gcgcggctgt tcgaggtggt cttcggcgtc ctgcgtcgcc tggcggacga
    20401 gcggccggtg gtcctggtgc tcgaggatct gcactgggcc gactcctcca ctcgcgacct
    20461 gctgcgcttc ctggttcgca acgcgcgcga cggccgcctg ctcttcatcg ccacctaccg
    20521 ctcggacgag ctgcaccgac gccacgcgct ccgccccttc ctggccgagg tgcagcgcgt
    20581 cgcgggcgtg gaggtgcgtg agctggatgc cttcgacgcg ggcgagatcg cggcgcaggt
    20641 gacggcgatc accggccgtc cggcccgcga cgaggtcgtc agcgcgctcc tggcacggtc
    20701 gggcggcaat ccgttcttcg cggaggagct gatcgtgggc ggggaggccg gcctggcgct
    20761 gccgcgcagc ctgcgcgaca cgctcgagga ccgcgtgcgc caggcgagcg ccgatgcgca
    20821 acggctcctg cgcgtcgctg ccgtcgcggg agaccgcgtg cagcacgggc tgctggccca
    20881 ggtgctggac ctgccggccg accgcctgca cgaggcgctg cgcgaggtga tcgagcacca
    20941 cctgctggtg ccgacgcgtc ccgacgaggt tccggggtac cgcttccgcc acgccctgct
    21001 ccaggaggtg gtgtacgagg ccctgctgcc cagcgagcgc acccagctcc acgccgcgta
    21061 cgccgcggtc ctggaggcgg acccgcagct ggccctcggg catgcgtccg gtgcgggcgc
    21121 gcagatcgcg catcactggg tgctggcgca tgacctggag cgcgcgctgc ccgccatgct
    21181 gcgcgccgcc cacgaggcca ccgcctccta cgcgttcccg gaggcgaagg cgttcctgga
    21241 gcgcgcgctc gagctgtggc cgcagatcgc cccggagcgc cttcccggcg atgtcgaccg
    21301 cgtgacgatc ctctccgagg cggcggaggc cgccgcccat gccggggacg cgcgacggtc
    21361 catcgacctg gtccgcgccg ccctggccga gacggatcca ctgggtgagc cgatgcgcgc
    21421 cggggtcctc caccatcgac tggcgtggta cctcaacgag tcgggcgact ggcagtccgg
    21481 cgtcgccgcg ctcgaccgcg cggtggccct catcccgatc gatccgccga cgcccgagcg
    21541 ggcgcgggtg ctgtccgacc tggcccactc cctcatggtc cgtggtcggt acggagattc
    21601 gctggcgatc gccgaggcgg cgctgtcgat cgcgcgcagc gtggaggccc gcgtggccga
    21661 ggcccgcgcg ctcaatgccc tcggcctgga cctcgcctgc cgctcggact tcgagcgcgg
    21721 cctgcccatc ctgcgcgagg gcttcgacct cgcggtcgag atgggcgatc cgctcgccat
    21781 cttcctgagt gccgccgggt acggctgggc gctcgacgag agcgcgcgcc acgccgaggc
    21841 cctcgagctg gcgcgcgagg cctccgagcg catccggctg ctgggcgccg aggcacgatt
    21901 cggcgggcag ctggcgtcca aggtggcgcg cgcgctgtac gacctcggac gctggaccga
    21961 ggcgctgcac atcatcgacg acaccctcgc cgcgagcccg acgcgctacg cggtgcgctg
    22021 gctgctctcg aaccgcatcc ggatcctggt gcaccgcggc gagctcgagg cggccaagcg
    22081 ggacatcgcg acgtacgacg cgctgggcga gcgggtgatc gggccggatc cggacctggt
    22141 caagcggcgc cgggccgagc tgagcatcgt cgccggaacg ccgacggccg cccggggcct
    22201 ggtgcgggag gtcctcgacc agctggtcga gccggagctc gacgcggacg cgcggccgct
    22261 cctgctggtg ggcctgcgcg ccgaggcggt cgaggcggac ctggcgcgcg ctggcgggtc
    22321 ggccgagcgg cggcgcgcgg ccgcgcgtcg ggcggacgag ctgctcacct ccatgcgcgc
    22381 ccacgtccag cgcgtgacga cctccgcctg gaaccccgca ccggtgatcg atgcggaccg
    22441 cgtcctggcc gaagcactcg cctcacgcgc tcggggcgag accgaggtcg agctgtggca
    22501 ggacgccgtc gccgggaggc gccggctcgc gcgaccgcac gagttggccg ccgcgctcgg
    22561 cgatctcgcg gccgtggcgc tcgagtcggg acatcgcgac gtgggggccg cggcgctggt
    22621 cgagtcccac ggcatcgccg tggcgctggc cgcgcggccg ctgcgcgagc agctggaggc
    22681 cctggccagg cgggccagca tccagctgcc cggcgtcccg tcggccgatg acgccgcggg
    22741 gaagctcggg ctgacccggc gcgagcgcga ggtgctcgcc ctcctggcgg atggacgcag
    22801 caaccgccag atcggcgagg ccctgttcat ggccgagagc acggcgggcg tccacgtcag
    22861 caacatcctg ggcaagctcg gcgtgtcccg gcgcaccgag gcggccgccc tggcgcatcg
    22921 gctcggcctg ttcggcgtgc tgtagccgtt cgcgccgctg cggccggcga gccgcctatg
    22981 ctcgtcggtg catgctcgac gcctcgacgc gccgcacggg cggctcgctc ctcgccatcc
    23041 tggccggcct ggcactgctc accgccgtgg cgttgctgcc ggacctcacg ccgagcgacg
    23101 cgctgcccgt cgtcggtcag tccagcgcgc aggccgtcat cgtccaggcg cttccgcccg
    23161 atgccaccgg ggctgcccgc ttcgcggtcg agctgcgcag cggcgacgac gcggggacga
    23221 cgatcgaggc catcgtccag gacggctcag cggcgcttcc cggatccgcg acgcgggaac
    23281 cgtacgaggt gggggacgag gtggtcgtca cgcagttcgc cggcgaggcg ggtgggttcg
    23341 ccgtgatcag cgatccgtgg cgcgtgccgc tgctgggcct ggtggcggca ctgttcgccg
    23401 cggtcgtcct ggtcgtcggt ggactgcgcg gcgcgcgctc actggtggcc ctggccctga
    23461 ccttcgcggt catcgtgaag ctggtggtgc cgctgctgct gcgcggcgtg gacccggtcc
    23521 tgctcgccgt cgtgggcggg atcgccatca ccgtcgccac gctgaccctc accgaggggt
    23581 tcggccgcgt gacgttggcc gcggtgctcg gcaccagcgc ggcgctgctg ctcaccgccg
    23641 tgctcgcggc ggcgttcacg gccgcggccg ccttcagcgc cctccagggc agcgaggaga
    23701 tcgcgttcct gattccgctg accggcgacc ggctcgacct gtccgggatc ctgcttgccg
    23761 cgaccgtgtt cggggcgctt ggcgtgctgg acgacgtcac cgtcacgcag gcggcgaccg
    23821 tggacgagct gcatcggtcc gatcccgccg ccggccgggg cgccgtcatc gcccgggcca
    23881 tgcgcgtcgg gcgctcgcac atcgccgcga cggtgaacac gctcatgctc gcctacctgg
    23941 gggtgggcct gccgctgctc ctgctcttcg ccctggccgg gcaggacccg ctcaccgtcc
    24001 tcaacgggga gatcgtcgcg gtcgaggtga tccgcgccct ggtcggaagc atcggcatcg
    24061 tcgcggccgt gccgctgacg accgcggtgg ccgcgctgct cggcgttccc gccaccccgg
    24121 cggcccgcag cgacgcatcg gcctgacggc ggtacactcg ccgtcgccca catcgagcac
    24181 ggagtcacgc atgaccgatc gcgtcccgcc catgttgttc accagtgaat cggtgaccga
    24241 ggggcacccc gacaagctgt gtgacgcggt ggccgatgcg atcctggacg acgtgctggc
    24301 ccacgacccc gacgcgcgca gcgcggtcga ggtggccacc accacggggc tggtcctggt
    24361 catgggcgag gtgagcacca cccactacgt cgacgtgcag gccgtggtgc ggcgcacggt
    24421 gcgcgagatc ggctacaact ccagccacct cgggttcgac tgggagacgt gcgggaccat
    24481 gactgccatc aaggagcagt cgcccgacat cgcgctcggc gtggaccgcg ccctggaggc
    24541 gcgcagcggg gagggccccg aggcggaggg tcccggcgcg ggcgaccagg ggatgatgtt
    24601 cggctacgcc tcggacgagt cgcccgagct gatgccgatg ccgatcatgc tcgcccaccg
    24661 cgtcgcgcgc cgcctggccg aggtgcgcaa ggacggcacg ctgcccttcc tgcgccccga
    24721 cggcaagagc caggtcacgg tcgagtacga cgccgaccac cggccgaggc gggttcacac
    24781 cgtgctggtc agcacccagc acgaccccga cgtcgacctc gccgacctgt cggccgcggt
    24841 gcgccggcac gtggtcgagg ccgccatccc cggcgagctg ctggacgacg ccacccgccc
    24901 cctggtcaac ccgaccgggc gcttcgtgat cggcggtccg atggccgact ccggcctttc
    24961 gggacgcaag atcatcgtcg acacctacgg cggcatgggc cgccacggcg gtggcagctt
    25021 cagcggcaag gacccgacca aggtcgaccg ctccggcgcc tacatggcgc gctatgtcgc
    25081 caagaacgtg gtcgccgccg gcctggcgcg gcgctgcgag ctgcaactgg cctacgccat
    25141 cggcgtggcg cggccgctgg ccatcaacgt ccagaccttc ggcaccggca ggctgcccga
    25201 cacgcgcctg gcccaggtgg tggagcaggt cttcgacctg cgccccggcg ccatcatccg
    25261 cgacctggac ctgcgccgcc cgatctaccg ccccaccgcc gcctacggcc acttcgggcg
    25321 cgacgacctg gacctgccct gggagcggac cgaccgcggc gacgacctgc gggccgcggc
    25381 gggctgacgg ccgatgggcg acgacgccgc cgcctcgcac cgacacccgc acccgcatcc
    25441 ccacgcgcaa tcccgccgtc cgtggggcga gcccccgccc ggaccgctcc gccggctgct
    25501 ccgccgcgtg cgcgggctgg tcggcatgga cgcgagcccg tccgcgcgcc ccggcatcgg
    25561 ggagctgccg gcggtgctgc ggcgtgaccc ggccgcgccg tcctacctgg cggcggagcg
    25621 tcgcggcgac catgaatcgg ccgcagccgc ggcatccgag gcgtcggagc gcgcgatcgc
    25681 cgacgaggcg tggtggcccg ccgacgcgtg ggcgcaccgc gccctgtggc actacgagca
    25741 ggcccagctc acgctcgagg cggcccggca ggcgcggcgc atcggtgacc tgcgaacggc
    25801 ggcgggcgac cccgagagcg cgcgtcgcta ctatgcggag gcgatcgacg aggcgcgcga
    25861 catcggcgcc gagcacgagc agggcctggc cgcgctggga ctcgggcgcg ccatgctcga
    25921 gctggggcag gtgacgcagg ctcgacgcct ggcgagcgcg gcgatcgacc tgctggagcg
    25981 ctcgaccgcg tcgacgctcg agctggacgc ggcgcacgcg ctgctcggac gagagcaggt
    26041 cgtcacgccg ggcgccggcg cgccggccac acgcgacgaa gcaacggagg agagctgatg
    26101 gcacgacgca cgatgcgggt cggcatcgtc ggcgcgggcg tcatggccga ggcgatcatc
    26161 gccgggctgg tcgcggaccg cgccgtgaca ccggatcttc tggtcgcctc gcacccgcgc
    26221 cgcgagcggc gcgaatccct ctcggagcga cacgggatcc gtgccgtggc cgccaaccgc
    26281 gacgcgctgg gggaggccga ggtcgtcgtc ctggcggtca agccgcagat gctgggccgc
    26341 gtcatgcgcg agctgcgcgg ccggctggac gccgagcagg tggtcgtctc gatcgtcgcg
    26401 ggtgccacgc tgcgcaccct ggtggacggc ctgcagcacg cgtcggtcgt ccgcgccatg
    26461 ccgaacacgc cgagccagat ccgtcgcggg atgagtgtgt gggccgcctc ggacgcctgc
    26521 accggacacc agcgggacct cgcgcgggcc gtgctgcgcg cgctgggcga cgagcgccag
    26581 gtcgccgacg agacgttcgt ggccatggcg acggcgctct ccggcacggg ccccacctac
    26641 ctgttcgccg tgatggaggc gctgatcgac gctggcgtcc acctgggctt cccgcgcgag
    26701 ctggcgcacg acctggtcgt cgagacgctg gtcggttccg ccgagtacgc caaacgctcg
    26761 gagctgcacc ccgcgcagct gcgcaacgcg gtgacgtcgc ccggtgggac gagcgcggcc
    26821 gccatctacg agctggagaa gggccggatc cgcaccgtct tctctgacgc ggtgtgggct
    26881 gcctaccgcc gcacggtgga gctgggcgag cgcctcgagg cggaggtcga gcgcgactga
    26941 tgttcgccat cgtcatggcc ggcgggtccg ggacgcggct gtggccgctc tcgcgtcgcc
    27001 gcaatccgaa gcagctgctg aacctgaccg gcgacaccag cctcctgcag cagacggtcg
    27061 cgcgcctggg caccctgctg cggccgcacg acatctacgt catcaccagc caggcccacg
    27121 tccgggccac ccaggagcag ctgccccagc tgcccgccga caacgtcctc ggggagccgc
    27181 tggcacgttc gacggccgtc gccgccggcc tggccaccgt cctggcgcgc cgcgagagcg
    27241 acgaggtggc gctcgtgctg cccgccgacc actacgtcgc ggacgaggac gcgttcgtcg
    27301 ccgggctgcg cgaggcggcc cgcgccgcgg agcgcggcta cctggtgacc ctgggggtgg
    27361 tgcccaccca cgcggcgacc gggtacggct acatccgggc cggtgcggcg ttccacccgt
    27421 cgtcgcgcac ggctccggtc gaggccttcg tcgaaaagcc cgacgccgac cgcgcccgcg
    27481 acttcctggc cgacggcagc tacctgtgga acgcgggcat cttcgtgtgg cgtgtgtacg
    27541 ccttccggca ggcggtcgag cggttccagc cacgcctggc cgaggcgctc gaccgcgtcg
    27601 gcgcgctgca ccgcacgccc ggctggatga gcgaggtccg cgagatcctc gagccgatcc
    27661 cggccatcac catcgacacc ggcatcgccg agccggcggc ggccgagggc cgcatggcgg
    27721 tcgtgccgct cgaggcgggc tggtcggaca tcgggtcgtg gtccgcgttg ctggaggcgc
    27781 tgtcgcacgc ccgcggcgcg gacctggtcg ccagcggccg ccacctcgac cgcggttcgc
    27841 acaacgtgct ggtgcacggc ggcgaccggc tggtggtcac ggtcgggctg gaggacgtga
    27901 tcatcgtcga cacgcccgac gcgctgctgg tctgcagccg cgaacgcgcc gaggagatca
    27961 agccggtcct cgacgagatc gcgcgtaccg ccggcgacca gtacctgtag ccgggcggcg
    28021 aatgcgcgct cttgcgtggc tcgtgggcaa ggcgatggca gcgtacgtcc gcctggtcgc
    28081 cgcgacatgc cgcatcgacg ggccgccggt gtccgacgag caggtggtcc tggccttctg
    28141 gcacgagtac aacctcgccg ccgccgtggc ggcgcatcgg ctgcggcgcc ggcaccacca
    28201 cgtcagcttc agcacgcgga ccttccgggg ccgcgtcatg aactcgatgc tggcgggact
    28261 cgactcgggc gccgtgccgc tgcccgacga gacccagcgc tccgagtcgg cgcgcctggc
    28321 ccgcgagctc gcgcggctcg ggcgcgaggg cagctcgctg gccgtcagcc cggacggtcc
    28381 gttcgggccg taccgccgcg ccaagcccgg tgcgctgatc gtcgcccggc acgccgggct
    28441 gccgctgcag ccgtgggccg tcgtcgcgcg gccgccattg cgcctgcggc gccgctggga
    28501 ccggcacctg gtccccttgc cgttcggacg cctgcgcgtc gtggaggggg agtcgctccg
    28561 ggtggggcct cgcgacgcgc tgcgcccgct ggtggcgcgg ttccagcagg cgctcgactc
    28621 gacctcggcg cgcgcggatg ccgcggagag ctgacgccgg tacgatggac gcacacacac
    28681 cgagcgatcc gttgagagag cgagtgccca acgcgtgacg aagatcaagt tcgggaccga
    28741 cggctggcgg gcggccatcg ccgaggacta cacgttccac aacgtgcgac gctgcgcccg
    28801 cggcgtggcg gagcacctgc agcagacggg cgccgcggat cgcggcgtcg tcgtctgcca
    28861 cgaccggcgc ttcgcgtctg agcacttcgc ccgggcctgc gtcgaggtcc tggccgcgca
    28921 cggcatccgc agcttcacgc cccccgaggc ggtgcccacc caggtcggca gcttcttcac
    28981 cagggagctg aacgccggcg ccgggatcgt gatcaccgcc agccacaacc cgtggaccga
    29041 caacggcttc aaggtgaagg ccgatacggg tggtgccgcc gccccggaga tgctcgccgc
    29101 catcgaggcg accatggaca cgcacccgga ggacgagctg ccgccccggc gcgaccacga
    29161 cgacgcagtc gaggccggcc tggtcgagat cttcgacccg tacccgaagt tccgtgacca
    29221 gctggcgagc atcgtcgaca tcgagaagat ccgggcggcc gagaagcggg tgctggtcga
    29281 gaccctctac ggctccggat ccggctggta cacccgcctg ctcggcggcg gccgcctcac
    29341 cgtccgcgag ctccacaccg agcgcaaccc ctacttcggc ggggtcaacc ccgagccgat
    29401 ccgccccaac gtcgacgagt ggctggagga gatcccgcgc tggggcgccg acatcggcat
    29461 cgccttcgac ggcgacgccg accgggtcgg catggccacg gaggagggtg tcttcgtcaa
    29521 ccagctgcag gtgtacggcc tgctgtactg gtacctgctc gacgtgcgca accagatcgg
    29581 cccggcggtc tacaccgtca ccacgacctc gatggccaag cgcctggccg agatctacgg
    29641 cacccgggcc tacgagacgg gggtcggctt caagtacgtt ggcccgaaga tgaccgagac
    29701 cgatgcggtc atcggcggcg aagagtcggg cggattcggg ttcggcatgc acatccccga
    29761 gcgcgacggc ctggcgtccg gcctcttcct cctcgacctg tggctgacga agggcaagcg
    29821 ggcgtccgag gtgctcgccg agctccaggc gctggcgggg ccgagctact acaaccgcat
    29881 cgacatccgc ttcgcgcgcg agggctacga ccaggtcaag accgatacgc tggcgcgcct
    29941 cgcggagcac gcgccaaccg agctcggagg gctggaggtg gtggagcgca aggacctcga
    30001 gacgggcgac ggcttcaagt actaccgctc cgacggctcc tggctcctga tccgcttcag
    30061 cgggacggag gcgctgctgc gcgtgtacgt cgaggcgcgt tcgcccgagg acgtcgacgc
    30121 cctgctggcc gagggccgcc gcatcgccgg cgcccgctga tggacgctcg cgacttcttc
    30181 ggctacaagc acgcggacga ggagccgggg cggcggcccg agaccgacga cgggacggag
    30241 gaggaccgcg tcccgacgtt cgaggaggcc gtgacgctcg acgatcccga gaccctgcgg
    30301 cgcatcgacc cgcaggacat gctcgggaag atcatggcga tgccggcgca gctcgcccag
    30361 gcgcgccgcg tcgcggcacc gatccgtctc gagcagcgcc accgggacgt ggacgccatc
    30421 gtcgtgctgg ccatgggcgg ctccgccatc ggtgccgagc tggtcgccgc ggcggcgggg
    30481 gagcggctgc gggtgccgct catcgtccat cgcgactacg gtctcccggc cggcgtcggc
    30541 gatcgcaccc tggtgatcgc ggcgagccac tccggcgaga cggccgagac gctctccggg
    30601 ttcgccgccg ctcgtgctcg cggtgtgccg ctggccgtca tcaccaccgg cggcaggctg
    30661 gcggccgccg ccaccgaggc cggggttcca ctgctccagt accgcctcgg cgggcagccg
    30721 cgcgccgcga tcggcttcgg ggtcggactc gtccacgagc tgctgtcggg agccgacctc
    30781 atcgtggacg gcgacccgct gggtccggtg gtgcaggcgc tcgaggagct gctcgagcgc
    30841 atcggtccgg gcgtcgagac ttcggcgaac ccggccaagc agctggcgtg ggccgcgttc
    30901 gggcgcatcc cggtcgtgta cggccacggc agcatggcgg cggtcgcgca ccgctggaag
    30961 acgcagctga acgagaacgc caaggcctgg gctgcctggg agccgatgcc cgaggcgaac
    31021 cacaacgcca tcgagggcag cctcaatccg cgcgagctga gcgacgcgct gtacgtggtg
    31081 cagctgcgcg atgccgacga gccggaggag atcggccagc gctaccgggt ggtcgaggag
    31141 ctgctcggcg agcgggcgac gaaccgctcg caggtccggg cggagggtcc gtcggcgctc
    31201 gcgcgcgtgc tgaccgcggt cgcgtacggc gacatgttca gcgcgtatct cgcgatcctc
    31261 taccagacgg acccgacgcc ggttacactg ctggcgatgc tcaaggagcg gctcgccagg
    31321 gccactggat ccgacccgac gggcgccgac tcgtagggcg ccgggttccg gcgccatgga
    31381 ggtaaccgcg acgtgcaatc gagcatgatc ggcaaggtcg agaaggccat gcgctacgcg
    31441 cacgagcctg accgcgtgaa gttcgcgtcc ttccgcgcca gcttcgccgg cgacaacggc
    31501 acgcacaccg tcaccctcga cgccgattcg tggcactgcg actgccacct cttcgaagcc
    31561 gccggcggct gcacgcacac cctggcgatc cagaagatgc tcgacccgat gctcaccgac
    31621 gccgcgcgcg agacgccgct ctacgcgcac gccgacatcc gcgacgagga ggccgtgccg
    31681 gcctaggtcc ttcgccgttg ccggggcccg ccgacgaccc gtcgccgggc gggccggcgt
    31741 ggccaccggc agctcatgac gcatcagctc ctcgccgtcc tcggatcgat cgcccacggt
    31801 atccggagct tcatcggcgc catcctcggc ctcctggggc tcgcgcgctt cgcccgcttt
    31861 gccgtgccct tgctggtggc ggccctcgcg gtcgccgccg tcgtcagcgc gcgggatacc
    31921 gcggtcatcc tcgggtcgcg cccgaccgtg accgatgcga cgctgcccga cgtggcgtcg
    31981 cataccgacg aggtcggggg ctcggtgtgg tacgccttcg acgcgctcat cgcccagcag
    32041 agcttcccct ccccgacgga ggcgggcacc ttcttctacc tcgcgctgga tcccgaggac
    32101 ccgtcgcagg gcctgctggt gcgctcgccg ctcaacgacg cgttcttccg ccagcgcgtg
    32161 ctgagcgccg atatcgtcga agattccgac accgtggcca ccgccctcga cgccttcggg
    32221 acgctccccg cgggcttcga ggtcgaccgc gtccggctgc tcgacgagac ctcggccggc
    32281 ggtgacctcg ccgacgcgtt cgtcccctcc cagctggccg acgaggcgtc cggcgagaac
    32341 ctgctggtca ccggccgggt cgtgtcgccg gcgaccttcg gcgtctgtgg cgagccggac
    32401 ggctgccagg cggcgggggc gcgctacctc tacctcttcg ccgatgcgga gggcggccac
    32461 gccttcgtcc tgcgctcacc gcatcctccg gccgccatcc cggtccgcct cgagggcctg
    32521 tacctgcgcg acacgttcga cctggcgccc gtcgtcgaga gcgactggtt ccgctccatc
    32581 gaggcggagg tgcccaccgg ccgggccttc caggccaaca cgcggccgcc catcacggtc
    32641 gaggcgtcct gggtgccgac catcgtcttc gcggccctcg ccctcctgct gctggccagc
    32701 cacctcgccg gctatcccgt cttcggtcgc gcccggcgac aacccgagcc cggccactcg
    32761 ctgggcgtcg gcgagggcgt cgacgtcgcg atcaccgggc ggatggcgcg cgaccgcgcc
    32821 accctccagc tcgaccgcag cccaggagcc ctcgagcggc tgccgatccc cgagctcgcg
    32881 ctgcggatgt ggcgctacgg tctgctcagc ccggacctga gccgccagga ggcggagcgc
    32941 cgcttcgtcg aggaggcggg cgcatccggc aaccgcctcg tcgtccacga gcgcgaccag
    33001 tcggccctgg tggtgctggg ccgcggctcg ggcgcggccg tccaggccgg acgtctgcat
    33061 cggctcgggc gcagcacgcc ggcggtccac gtccgcgacg atcggaccga tgcgcacctc
    33121 gagcttcgca gcgccgcgga gcgcgaccgg gtcgccgccg agatcctcgc cgaggtcggg
    33181 gagtccgcgg actgaccgac ggcacccgcc ggtgctagga ttcgccaggt caactgaaga
    33241 ccagcgacga gtgccgctcc cgaaaggcga gcggttcaat ggcgaagccg gtgagaggcc
    33301 ggcgcagtcc cgctactgta tccgcgccct gcggcgcggg agccaggtcg accagctcgg
    33361 tcgcgatgtc accaccctcg agagaaaggg aacggcatca ccgtggctcc ccgcacccgt
    33421 caacgccgcg taacgctcct cgcggccgcc ctcgtcgccc tgctcgcgac ggcctgcagc
    33481 accccgagcg gcacggccgc gccatcgacc gatccgacgg cctccgacct cgcctccccg
    33541 ggctctcaga tgaccgtgac cgatgcgcgc ggggcggagg tgacgatcga cggggtgccg
    33601 gagcggatcg tcagcctcgc cccgtcaaac acggagatcg tctgcgccct caacgcctgt
    33661 gatcggctcg tcggcgtgac cgacttcgac gactacccgc ccgaggtggc cgacgtggcc
    33721 gacgtcgtgg tctcggccca ggtggacgtc gagcgggtcg tggacgccca gccggatctc
    33781 gtcctcgcgg cgggcaacga gctcacgccc accagcgtga tcacccagct cgagggcctg
    33841 ggcctgaccg tcatcgtcct ctaccccgaa tcactggacg aggtgaccga cgacatcgag
    33901 ctgctgggtg gcgtcctgga cgaagcggac gccgccgcct ccctggtcgc cggcatgcgc
    33961 cagcgggtcg gagcgatcac cgccgccgtc gaagggctgg accggccgcg cacgctgtac
    34021 gaggtctact acgccgaggg cgtcacgtac acggccgggg agggcagctt cctcgcctcg
    34081 ctcatcgagc tggccggcgg ggagcccgtc accggcgacg cgcagggaac gctgccggcc
    34141 gaacagctgg tggccgcgga cccgcagctc gtcctgctcg gcaccgcctc gtacgatccg
    34201 tcgctcgcga cgccggccgc ggcgctggag gcggtgcggg cacggcccgg ctggtcggag
    34261 ctgtcggcgg tacgtgacgc cgaggtgctg ccgtacctcg acgacatcgt cacgacgcgg
    34321 ccgggtccgc gcatcgtcga cgggctcgag gcgctggccc gggccatcca ccccgaggcc
    34381 ttcgagtgag cggggcctga ccgatgcgcg ccaccaccgc cacccgcccg ctcgtgctgg
    34441 gcccgaccct gggccgcagc agtgtcgtgc tgggcctcgg agcggtgctg ttggtcgcgg
    34501 cagccgcggt cgccctggcg gcggggacga gcggcgtcgc gccgggcgat gcgctggcca
    34561 tcgtcgccca ccgcctcctg ggcctcggct ccgtgagctg gccggcatcg gccgagacga
    34621 tcgtgtggga gctgcggctg ccgcgcatcg tggcggggat ggtggtgggc gcgggcctcg
    34681 cctcgtccgg ggtggtcttc caggcgctcc tccgtaaccc gatggccgac ccgtacctca
    34741 tcggcacggc ggccggcgcg tcgctcgggg ccgtcgcggc gctgctcctg ccttccctgc
    34801 tgccgatcgc ggcgaccgcc tggctcggca tcggcgcggt ccaggtgctg gccttcgccg
    34861 gtgggctcgc cacagttctc ctcgtcttcg ccgtggcgcg cggccgcatg ggcacccccg
    34921 tcgtcacgct gctgctggcc ggctacgccg tctcgtcgct gctctctgcc ggcgtcgcgc
    34981 tgctcatgtt cgtcgccggg gaccgcctgg ccgccgtcgt cagctggctg ctcggctcgc
    35041 tcggcggcgc gagctggccg cgcctggccc tcgccgcgcc gctgatcggc atcagcttcg
    35101 tcctgctcct ggttcgctgg cgcgccctca acgcgctgct cctgggcgag ggccacgccg
    35161 ccaccctggg catcgacgtg gagcgcgaga agcggcggct gaccgccctt gcggcactgg
    35221 cgacgtccgc cggcgtcgcg atctcgggga ccatcggctt cgtcggcctg gtcgttccgc
    35281 acctgctgcg gctcgccttc gggcccgacc accggctgct gctaccggcc agcacgatct
    35341 acggcgccgt cctcctcgtc ctggccgata ccggcgcgcg cctggcgggc ggtatcccgg
    35401 tcgggatcgt gaccgcgctc atcggcgcac cgttcttcat ctggctgctg cgtcggtccc
    35461 tggcggtgcc gcgtggctga tctcctgccc gttctcgagc tgacggatgt gcgcgccgga
    35521 tacggacgcc gcagggtcct ggacggcgcg agcctgagcg tggcggcggg ggaggtggtg
    35581 gccgttctcg gtaccaacgg cagcggcaag agcacgctgc tccgagtcgc cgccggcctg
    35641 ctgcgtccgt ggtcgggcag tgtccgcatc ctcggccggc cgtccggggc gtggcccagg
    35701 cgcgacctcg cgcggcgggt cgcggtgctg ccccaggcgc tcgagctgcc cctgggcttc
    35761 cgcgtcgcgg aggtcgtcgc catgggccgg atcccgcacg cgcgcgccct cttcgccgac
    35821 gacggcgacg acggcagcgc ggtcgcggcc gcgctgcgcg acgccgacgc gaccgacctc
    35881 gccgaccgcg aggtgtccga gctgtccggc ggggagcgcc agcgcgtcct ggtcgcgatg
    35941 gcgctggccc aggagcctga tctgctgctc ctcgacgagc cgaccctcca cctcgacctc
    36001 gcgcaccagc tgacgctggt ccagaccctg gcccggctgc gccgcggg
//
LOCUS       DASTYP010000147         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1468400, whole genome shotgun sequence.
ACCESSION   DASTYP010000147 DASTYP010000000
VERSION     DASTYP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468400"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2995
                     /gene="rpoC"
                     /locus_tag="VFV59_06810"
     CDS             <1..2995
                     /gene="rpoC"
                     /locus_tag="VFV59_06810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5325576.1"
                     /translation="GRAIAGTGNHRLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIV
                     VGPELKLHQCGLPKKMALELFKPFVMRQLVEKGFAHNIKSAKRIVERVRPEVWDVLEE
                     VIKDHPVLLNRAPTLHRLGIQAFMPTLVEGSAIQIHPLVCFAFNADFDGDQMAVHVPL
                     STAAQREAKELMLSTRNLLSAADGSPVVSPTHDMVLGCYYLTVQVDDEKGAGKAFASA
                     DEAIMAAQLGHVHVQAPVKVELDLYDGTDEDGTVRTHREVVDTTAGRVIFNTVLPPEM
                     GFSNEVMDRKALKAIIARCYRDLGPTATASLVDGIKAVGFRYATAAGITIGVEDLAVP
                     KEKAKLLTDAEQKVTDIEREFRRGFITEDERYTQTVEVWRKATDDVTQKMLSGLDERG
                     SIMLITNSGARGSVTQVHQLAGMRGLMADPSGRIIDLPIRSNLREGMSVLEYFISSHG
                     ARKGLADTALRTADSGYLTRRLVDVAQDVITREDDCGTDLGTWITREESDQIPEPFVE
                     RLIGRMAAIEIADEKSGEVIVERNAEIGEAAIERIEELGIDRVSVRSPLTCAARHGVC
                     RMCYGRNLATGELVELGQAVGIIAAQSIGEPGTQLTMRTFHTGGVAGEDITQGLPRVE
                     ELFEARIPKGQATMTEIDGTVEVPPASGDQPITVRVTHRDAYDTSFKLTPQHEILVAQ
                     GDEVTEGQLLARLGEGEELVEVKAPSAGRITKKGNTLTLHTEEVDVREYQVPHSARLR
                     VGDGDEVSAGDPLTEGSLNPKDLLEIKGVDTVQRFLVAEVQKVYRSQGVTISDKHIEI
                     IVRQMLRKVTVDNPGDTELLPSELIDRFEFEETNNRILAEGGEPATAQTVLLGVTKAS
                     LNTSSFLAAASFQETTRVLTEAAINGAKDHLIGLKENVIIGKLIPAGTGAEARRLQAE
                     RAAQLSPEEAEQEREAAAARAFLSGDPDTDQSGAVETDEAEAAAEAEGDTIAELMGAG
                     DNPEMEAAVEEQVFEELTEEEPAKS"
BASE COUNT          565 a         1084 c         1122 g          412 t
ORIGIN      
        1 cggccgggcg atcgcgggca ccggcaacca tcggctcaag agcctgtccg acatgctcaa
       61 gggcaagcag ggccggttcc gccagaacct gctcggcaag cgcgtcgact actcgggccg
      121 ctcggtcatc gtggtcggcc cggagctgaa gctgcaccag tgcggcctcc cgaagaagat
      181 ggcgctcgag ctgttcaagc cgttcgtcat gcggcagctg gttgagaagg gcttcgccca
      241 caacatcaag agcgccaagc gcatcgtcga gcgtgtccga cccgaggtgt gggacgtgct
      301 cgaggaggtc atcaaggacc acccggtcct gctgaaccgc gcgcccacgc tgcaccgcct
      361 gggcatccag gccttcatgc ccaccctggt ggagggctcg gccatccaga tccatccgct
      421 cgtgtgcttc gcgttcaacg cggacttcga cggtgaccag atggccgtgc acgtcccgct
      481 ctcgaccgcc gcgcagcgcg aggcgaagga gctgatgctc tcgacccgca acctgctgtc
      541 ggcggccgac ggctcgccgg tggtgtcgcc gacgcacgac atggtgctgg gctgctacta
      601 cctgacggtc caggtggacg acgagaaggg cgccggcaag gcgttcgcca gcgccgacga
      661 ggcgatcatg gcggcgcagc tgggccacgt gcacgtgcag gccccggtca aggtggagct
      721 cgatctctac gacgggaccg atgaggacgg caccgtccgc acccatcgag aggtcgtcga
      781 cacgacggcc ggacgcgtca tcttcaacac ggtcctgccg ccggagatgg gcttcagcaa
      841 cgaggtcatg gaccgcaagg cgctgaaggc catcatcgcg cgctgctacc gcgacctcgg
      901 gccgaccgcc acggcgagcc tggtggacgg catcaaggcc gtcggcttcc gctacgccac
      961 cgccgcaggc atcaccatcg gcgtcgagga cctggccgtt cccaaggaga aggccaagct
     1021 gctcaccgat gcggagcaga aggtcaccga catcgagcgc gagttccggc gcggcttcat
     1081 caccgaggac gagcgctaca cgcagaccgt cgaggtctgg cgcaaggcga ccgacgacgt
     1141 gacccagaag atgctgtccg ggctcgacga gcgcggctcc atcatgctga tcacgaactc
     1201 cggcgcccgc ggctcggtga cccaggtcca ccagctggcc ggcatgcgcg gtctcatggc
     1261 cgacccgtcc ggccggatca tcgacctgcc gatccggtcg aacctgcgcg aggggatgag
     1321 cgtcctcgag tacttcatct cgagccacgg cgcccgcaag ggcctcgcgg acaccgcgct
     1381 gcgcaccgcc gactccgggt acctcacccg gcgcctggtg gacgtggcgc aggacgtcat
     1441 cacccgcgag gacgactgcg gcacggacct cgggacgtgg atcacgcgcg aggagtccga
     1501 ccagatcccc gagccgttcg tcgagcgact catcggtcgt atggcggcca tcgagatcgc
     1561 cgacgagaag agcggcgagg tgatcgtcga gcgcaacgcg gagatcggcg aggcggcgat
     1621 cgagcggatc gaggagctcg gcatcgaccg ggtcagcgtc cggtcgccgc tgacctgcgc
     1681 ggcgcgccac ggcgtgtgcc gcatgtgcta cggccgcaac ctcgcgaccg gcgagctcgt
     1741 cgagctcggc caggcggtcg ggatcatcgc cgctcagtcg atcggcgagc ccggcacgca
     1801 gctgaccatg cggacgttcc acaccggcgg cgtcgccggc gaggacatca cgcagggcct
     1861 gccgcgcgtc gaggagctgt tcgaggcccg catccccaag ggccaggcga cgatgaccga
     1921 gatcgatggc accgtcgagg tgccgcctgc gtcgggcgac cagccgatca cggtgcgggt
     1981 cacgcaccgc gacgcgtacg acacgagctt caagctcacg ccgcagcacg agatcctggt
     2041 ggcccagggc gacgaggtga ccgagggcca gctgctggcc cgcctgggcg agggcgagga
     2101 gctggtcgag gtcaaggcgc cgagcgccgg ccgcatcacc aagaagggca acacgctcac
     2161 cctccacacc gaggaggtgg acgtgcgtga gtaccaggtc ccgcactcgg cgcgcctgcg
     2221 cgtcggcgac ggcgacgagg tctccgccgg cgatccgctg accgagggct cgctcaaccc
     2281 gaaggacctg ctcgagatca agggcgtgga caccgtccag cgcttcctgg tcgccgaggt
     2341 ccagaaggtg taccggtcgc agggtgtcac catcagcgac aagcacatcg agatcatcgt
     2401 gcggcagatg cttcgcaagg tcaccgtgga caaccccggc gacaccgagc tgctgccgtc
     2461 cgagctcatc gatcgcttcg agttcgagga gacgaacaac cggatcctcg ccgagggcgg
     2521 cgagccggcg acggcgcaga cggtgctgct gggcgtgacg aaggcgtcgc tcaacacctc
     2581 gtccttcctg gcggccgcca gcttccagga gacgacccgc gtgctgaccg aggcggcgat
     2641 caacggcgcc aaggaccacc tcatcgggct gaaggagaac gtgatcatcg gcaagctgat
     2701 ccctgccggg accggcgccg aggcgcgacg cctccaggcg gagcgggcgg cccagctgtc
     2761 gcccgaggag gcggagcagg agcgcgaggc cgccgcggca cgcgcgttcc tgtcgggcga
     2821 tcccgacacc gaccaatccg gagcggtgga gaccgacgag gccgaggcgg ctgccgaggc
     2881 cgagggcgac acgatcgccg agctcatggg cgccggcgac aacccggaga tggaggccgc
     2941 cgtcgaggag caggtcttcg aggagctgac cgaggaggag ccggcgaaga gctgacgctc
     3001 cttcctccag accgacgcga agggcggccc caatgggccg cccttcctgc gtgtctgggg
     3061 ggtgttaacc gggtcgccgc ccggggattc tcagcgcttg cgtggctggt tggggaatcc
     3121 gatgccccgg tcgagcagga agagcgcgaa cgcgaagaag ccgatcacga acaggaagaa
     3181 cgg
//
LOCUS       DASTYP010000148         7531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1067668, whole genome shotgun sequence.
ACCESSION   DASTYP010000148 DASTYP010000000
VERSION     DASTYP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067668"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFV59_06815"
     CDS             <1..251
                     /locus_tag="VFV59_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU5325577.1"
                     /translation="FNASGVEVLERMDEPVDPAYVDELVRRFDDFRRAYEPDGLRTEE
                     FDGFPPTVRTLRAFIGSYHDLLAAVTDAQLPNPDVAGA"
     gene            254..547
                     /locus_tag="VFV59_06820"
     CDS             254..547
                     /locus_tag="VFV59_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU5325578.1"
                     /translation="MAYAIAATWRAREGEEREVRRILETVAPLNRAEPGCRMFIVHES
                     PDDPRTFFLYEQYDDEAAFQAHAASDHVRRHVLEDAVHRLDHRERTIYHTTDM"
     gene            558..1229
                     /locus_tag="VFV59_06825"
     CDS             558..1229
                     /locus_tag="VFV59_06825"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5325579.1"
                     /translation="MTELDHPTSPLARSVLSSQVKDRMLQWILEGELAPGSRIIETRI
                     ARQLGTSQAPVREALRDLATLGLVDVHPYRGARVRQPEPEELVEAMELRGELEAVAAR
                     SAARRMDEATLSEMRELLEAMRASASRDDAHAQAMQNSAFHTCVITASGNRTLQRLWS
                     MLEPFARTYLTATVPGADLHWLAERHVAILEALEARDPDGAAEAMRTHAREAERQVLA
                     TQEAG"
     gene            1234..2103
                     /locus_tag="VFV59_06830"
     CDS             1234..2103
                     /locus_tag="VFV59_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU5325580.1"
                     /translation="MSSFAYARPERLADVFGLWRDEPAARLLSGGTDLLVAIRKGRAR
                     PPLVIDLKHVQELAPGISEREGFLRIGASTVLSDMVADARVRAAFPALVEAALVVGSI
                     QIRNRATLTGNLCNASPAADTAPPLLVHDAVVEVTGPSGARRLTLDAFFVRPGRTALE
                     RSELVSAIELPIPERPSGGAFERMTRRRGVDLATVNLACAIGADGVTRLGFGAVGPRP
                     LLAVDDSGVLADPGASAEEQDAVLDRLAAVTSPISNVRASREYREAMLIVLARRALAT
                     ARRRLEEARHAGR"
     gene            2090..2584
                     /locus_tag="VFV59_06835"
     CDS             2090..2584
                     /locus_tag="VFV59_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5325581.1"
                     /translation="MPAAELRIGLDVNGHREELEVAVHHTLLDVLRDRLGLTGTKECC
                     AEGECGACTVLVDGEAVNSCLVLAVEVDGHRVTTIEGLGEGDRLSRLQDAFLECGAVQ
                     CGFCIPGMVMSAHYLLSQTPHPDATQIREGLSGNLCRCGGYNQICSAVALAASTTDTR
                     EDVE"
     gene            2581..4917
                     /locus_tag="VFV59_06840"
     CDS             2581..4917
                     /locus_tag="VFV59_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5325582.1"
                     /translation="MTDAELPAGRSEAITGNLPEHGGSAATPRASSIGRSVRRSGGVE
                     RVNGSQRYVADLHLEGALHVKLVHLDVARARIGSIERSAAMAVPGVRLVVTADDLPDP
                     IPRFGPGYTDRPLLATGETKYHGEPVAAVVADSLDAAEEAARLVRVQAEPLPTVLTIQ
                     QALDPSSPLVREPELRDPDDPLRESNTLLEWRFGWGDVDAAVADHVIENVYTAAATTH
                     FAIEPHAFMAAPDDDGVAVWSSIQHPYVLQRLVAATLDLPLSKVRVFAPDPGGGFGGK
                     QHAKYEPLLALLALRLGRPVRLVLTLEETFQAVRGPTFTIRVRSGFRNDGGISFQDIE
                     SNGLLGAYADIGHRVVSKSSYLACGPYRTPDARIHVRALLSHTPPATALRGFGSPQMA
                     WAFESQMDEAARLLGLDRVEIRLRNLARKGEQIVRGAGDTPADGEWDQAVRKAAQWIG
                     WDAPRPPGHGRGLAIGIKSSATTGASYAIVRLLHDGSATVMAGTSDMGQGARTLLAQI
                     AAEELGTTVDRVTVVMGDTAAAPYDMQTSASRSTVFMGTAVQNACNDVRARLAALAAE
                     TYGVPADQVEVGHGDVKLPGRQIDFVQLMHDAFGPMLGEVIGSGSRRGAYLADHPLGG
                     HAAFYEFSCTASEVAVDDETGEVRLLRHVTVSDVGKALNPTQVAMQDEGAAMMGLGQT
                     LMEHLVFDADSGRIENLGALDYRIPTTKDVPVEMASLVIENQDGPGPYGSKGTGESGL
                     LSTAPAIASAIADATGVVLRDLPLTPERVWRALRERRP"
     gene            complement(4914..5807)
                     /locus_tag="VFV59_06845"
     CDS             complement(4914..5807)
                     /locus_tag="VFV59_06845"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEU5325583.1"
                     /translation="MSDYHLHLHPHRPTPGAPPMGVYPPGYIDRYVEAALARGVTEIG
                     FTEHLYRCVESERVLGQWWTSDPREDLQAQIEAWLASERNLSLERYVEVVLDAKSRGL
                     PVRLGLEVDFVPGTEAAVLELLAPFPFDYLVGSVHWIGAWNFMRDTAPEEFERRGVAT
                     AFEQYFALETALAGSGMVDVLGHADVIKRHRLRPEGDLTPFYLPLVEAAAGSGTAIEI
                     SSAGLRYPVGQTFPSADVLPIFQRAGIPITLASDAHQPDQTAWGHDEVVAVARAAGYR
                     EYLRFDLRTARPTPLPDLAAP"
     gene            complement(5830..6702)
                     /locus_tag="VFV59_06850"
     CDS             complement(5830..6702)
                     /locus_tag="VFV59_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5325584.1"
                     /translation="MQQVTPNVRTATDIRGCNPSYVTTADGVVVIDTPQLPTRAVEMR
                     EEAASHGPIRYVVNTEHHVDHIFGNYYFKGAGRVVHHRGVAELFMVPAPILDPFAYAA
                     EAIPTDDPQGQAIFPEREVYYADPNKGRIVFTGDLTLRVGDHTFELIHTPGHTPGQLA
                     VHVPEERVVFTGDTVFSEVQTWLMTSDIDQWVAALDRIALLDVDHVVPGHGPVTTTAY
                     LATQKAVLLEWRAAVADAVAKGWSREETIQRISFADRYPVDIGQEYMMGYIQSNNAGA
                     LWDQLSAPFRTHGS"
     gene            complement(6717..>7531)
                     /locus_tag="VFV59_06855"
     CDS             complement(6717..>7531)
                     /locus_tag="VFV59_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5325585.1"
                     /translation="APAGDAFDMADELARFRLDGRVAVVSGGTGGIGRRVSIALAGVG
                     ARVAVLGRSAEASTELAGEIEAIGSEALLVSADVTRKADADRAIDRVLEAFGRVDIIV
                     NAVGGGAGTALYPAEDYPESEWNRILDLNLRSDLLPTQAAVRAMIAAGNGGRVLRVSS
                     VRGQLGINQGFSAYVAAKGAINALTRQQATEWAKHAITVNAIAPTFVATPQVESLLAD
                     EEFKRGLLARIPLGRVAATDDLVGAVLFFCSDASAFVTGQVLTIDGGLTATQ"
BASE COUNT         1075 a         2671 c         2734 g         1051 t
ORIGIN      
        1 gcttcaacgc gtccggtgtc gaggtgctgg agcgcatgga cgagccggtc gaccccgcgt
       61 acgtggatga gctggtgcga cgcttcgacg atttccgtcg cgcctacgag cccgacggcc
      121 tgcggacgga ggagttcgac ggattcccgc cgacggtgcg cacgctccgc gcgttcatcg
      181 gcagctacca cgatctgctg gcggcagtga ccgacgcgca gctccccaac ccggacgtgg
      241 cgggggcctg accatggcgt acgcgatcgc cgccacgtgg cgggccaggg agggcgagga
      301 gcgcgaggtt cgccgcatcc tggagacggt ggcgccgttg aatcgtgccg agccggggtg
      361 ccgcatgttc atcgtccacg agtcgcccga cgacccgcgc accttcttcc tgtacgagca
      421 gtacgacgac gaggcggcgt tccaggccca tgccgcgagc gaccacgtgc gacgccacgt
      481 cctggaggac gcggtgcatc ggcttgatca ccgcgagcgg acgatctacc acacgacgga
      541 catgtgacgg cggaccggtg acggagctcg accaccccac cagcccgctc gcccgcagcg
      601 tgctgagctc gcaggtgaag gatcggatgc tgcagtggat cctggaggga gagctggcgc
      661 cgggctcgcg gatcatcgag acgcggatcg ctcgtcagct gggaaccagc caggcgccgg
      721 tccgggaggc gctgcgcgac ctcgcgacgc tgggcctggt cgacgtgcac ccgtaccgtg
      781 gagcccgcgt ccgccagccg gagccggagg agctggtcga ggcgatggag ctgcggggcg
      841 agctcgaagc ggtggcggca cgcagcgcgg cgcggcggat ggacgaggcg acactcagcg
      901 agatgcgtga gctgctcgag gccatgcgcg ccagcgcctc ccgcgacgac gcccacgccc
      961 aggccatgca gaactccgcc ttccacacct gcgtcatcac cgcctccggc aatcgcacgc
     1021 tgcagcggct gtggtcgatg ctcgagccct tcgcccgcac ctacctcacg gccaccgtcc
     1081 cgggcgccga tctccactgg ctcgcggagc gccacgtcgc gatcctggag gcgctcgagg
     1141 cgcgcgatcc ggacggcgcg gcggaggcca tgcgcaccca cgcccgggag gcggagcgcc
     1201 aggtgctcgc gacccaggag gcgggctgag cccgtgtcct cgttcgccta tgcgcgcccc
     1261 gagcgtctcg ccgacgtctt cggcctgtgg cgcgacgagc cggcggcgcg cctcctctcc
     1321 ggcggcaccg atctgctggt cgccatccgc aagggccgcg cgcggccacc gctggtcatc
     1381 gatctgaagc acgtccagga gctggccccg gggatcagcg agcgcgaggg cttcctccgc
     1441 atcggcgcat ccacggtcct ctctgacatg gtcgccgacg cgcgggtgcg ggcggcattt
     1501 ccggcgctgg tggaggcggc cctggtcgtc ggatccatcc agatccgcaa ccgcgccacc
     1561 ttgaccggca acctgtgcaa cgcatcgccg gcggccgata ccgcgccccc gctcctcgtc
     1621 cacgacgcgg tcgtcgaggt caccggaccc tcgggagcgc gacgcctcac gctcgacgcg
     1681 ttcttcgtca ggcccggacg gacagcgctg gagcggtcgg agctcgtgag tgccatcgag
     1741 ctgccgatcc cggagcggcc gagcggcggc gccttcgagc ggatgacgcg ccgtcggggc
     1801 gtggacctgg cgacggtcaa cctggcctgc gccatcggtg ctgatggcgt gacgcgcctc
     1861 gggttcggtg cggtgggccc gcgtcccctg ctggccgtgg acgacagcgg cgtcctggcc
     1921 gatcccggcg cctccgcgga ggaacaggac gcggtgctgg atcgcctggc cgcagtcacc
     1981 tctccgatct cgaacgtccg ggccagccgc gagtaccgcg aggccatgct catcgtcctc
     2041 gcccgacgcg ctctcgccac cgcccgtcga cggctcgagg aggcccgcca tgccggccgc
     2101 tgagctgcgc atcggcctcg acgtcaacgg gcaccgcgag gagctcgagg tggccgtgca
     2161 ccacaccctg ctcgacgtcc tgcgtgatcg gctcggcctc accggaacga aggagtgctg
     2221 cgccgagggg gagtgcgggg cctgcaccgt cctggtcgac ggagaggcgg tcaactcgtg
     2281 cctggtgctg gccgtggagg tcgacgggca tcgcgtcacg accatcgagg gcctgggcga
     2341 gggcgaccgc ctgagccggc tgcaggacgc cttcctggag tgcggagcgg tgcagtgcgg
     2401 cttctgcatc cccggcatgg tcatgtccgc gcactacctg ctctcgcaga cgccgcaccc
     2461 cgacgcgacg cagatccgcg agggcctctc cggcaacctc tgccgctgcg gtggctacaa
     2521 ccagatctgc agcgccgtcg cgctggccgc ctcgacgacc gacacgcggg aggacgtcga
     2581 atgaccgatg cggagctccc cgccggacgc agcgaggcga tcacggggaa ccttccggag
     2641 cacggtggtt cggcagcgac gccccgcgcc tcgtcgatcg gccgctccgt ccgccgctcc
     2701 ggcggtgtgg agcgggtcaa cggcagtcag cgctacgtcg ccgacctgca cctcgagggc
     2761 gcactccacg tcaagctcgt ccacctcgac gtcgcccggg cgcggatcgg gtccatcgag
     2821 cggagcgccg cgatggcggt ccccggggtc cgtctggtgg tcaccgcgga cgacctgccc
     2881 gacccgatcc cccgcttcgg ccctggctac accgaccgcc cgctgctcgc cacgggggag
     2941 acgaagtacc acggcgagcc ggtggccgcg gtcgtcgccg acagcctgga tgcggccgag
     3001 gaggcggcgc ggctcgtccg cgtccaggcg gagccgctgc cgacggtcct caccatccag
     3061 caggcgctgg acccgtcctc gcccctggtg cgcgagccgg agcttcgcga ccccgacgat
     3121 ccgctgcgcg agtcgaacac gctgctcgag tggcgcttcg gctggggcga cgtcgacgcg
     3181 gcggtcgccg accacgtcat cgagaacgtg tacaccgcgg ccgcgaccac gcacttcgcc
     3241 atcgagccgc acgccttcat ggcggccccg gacgacgacg gcgtcgcggt gtggagctcg
     3301 atccagcatc cgtacgtgct gcagcggctc gtcgccgcca cgctcgacct gccgctctcg
     3361 aaggtgcgcg tcttcgcgcc cgatcccggc ggagggttcg gcgggaagca gcacgccaag
     3421 tacgagccgt tgctcgcgct gctggcactc cggctcggcc gtccggtgcg cctggtgctg
     3481 acgctggagg agacgttcca ggccgtccgt ggcccgacct tcacgatccg cgtccggagc
     3541 ggcttccgaa acgacggcgg gatcagcttc caggacatcg agagcaacgg gctcctgggc
     3601 gcgtacgcgg acatcggcca ccgggtcgtg tccaagagca gctacctggc gtgcggaccg
     3661 taccgcacgc cggatgcgcg gatccacgtc cgcgcgctgc tgtcgcacac gccgccggcc
     3721 accgcgctgc gcggcttcgg cagtccgcag atggcgtggg ccttcgagtc gcagatggac
     3781 gaggcggcgc gactcctggg cctggaccgg gtcgagatcc ggctgcgcaa cctggcgcgg
     3841 aagggggagc agattgtccg cggcgctggc gacaccccgg ccgacggcga gtgggaccag
     3901 gctgtccgga aggcggccca gtggatcggc tgggacgctc cgcggccgcc gggccatggt
     3961 cgcggcctcg ccatcggcat caagtcctcg gcgacgaccg gggcgtccta cgccatcgtg
     4021 cggctcctcc acgacggcag cgccaccgtc atggcgggaa cgtcggacat gggacaggga
     4081 gcgcggacgc tgctggcgca gatcgcggcg gaggagctgg ggacgacggt cgatcgggtg
     4141 acggtggtca tgggcgacac ggccgcggcg ccgtacgaca tgcagacgtc ggcgagccgc
     4201 tcgacggtgt tcatgggcac ggcggtgcag aacgcctgca acgacgtgcg cgccaggctc
     4261 gccgcgctgg cggcggagac gtacggcgtg ccggccgatc aggtggaggt cggtcacggc
     4321 gacgtgaagc tccctggccg ccagatcgac ttcgtgcagc tcatgcatga cgccttcggc
     4381 ccgatgctcg gcgaggtcat cggctccggc tcacggcgcg gggcgtacct cgcggaccat
     4441 cccctcggcg ggcacgcggc gttctacgag ttcagctgca cggcctccga ggtcgcggtc
     4501 gacgacgaga cgggcgaggt gcgcctccta cgccacgtga ccgtctccga cgtcgggaag
     4561 gccctcaacc cgacccaggt ggcgatgcag gacgagggcg ccgcgatgat gggcctgggc
     4621 cagacgctga tggagcatct cgtcttcgac gccgattcgg gccgcatcga gaacctcggc
     4681 gcgctcgact accggatccc gacgaccaag gacgtgccgg tggagatggc gtcgctggtg
     4741 atcgagaacc aggacggccc gggcccgtac ggctcgaagg gcacgggcga gagcgggctg
     4801 ctgtccaccg ccccggccat cgcctccgcc atcgcggatg cgaccggcgt ggtcctgcgc
     4861 gacctgccgc tcacgccgga gcgggtctgg cgagcgctcc gcgaacgccg accctaggga
     4921 gcggcgaggt ctggcaatgg cgtcggccgg gccgtccgta ggtcaaagcg caggtactcg
     4981 cggtaccccg cagcgcgggc gaccgcgacc acctcgtcgt ggccccacgc cgtctggtcc
     5041 ggctggtgcg catcggaggc gagggtgatc gggatcccgg cgcgctggaa gatcggcagg
     5101 acgtccgcgg acgggaatgt ctgcccgacc gggtagcgga ggccggcgct cgagatctcg
     5161 atcgccgttc cgctgccggc ggctgcctcg acgagcggga ggtagaaggg tgtgaggtcg
     5221 ccctcgggcc gcagccgatg gcgcttgatg acgtcggcgt ggcccagcac gtcgaccatc
     5281 cccgacccgg ccagggcggt ctcgagcgcg aagtactgct cgaacgccgt cgccacgcca
     5341 cgccgctcga actcctcggg agccgtgtcc cgcatgaagt tccacgcccc gatccagtgg
     5401 accgagccga ccaggtagtc gaacgggaac ggcgccaaca gctcaaggac ggccgcctct
     5461 gtgcccggga cgaagtcgac ctcgaggccg agcctgaccg gcagcccgcg gctcttggca
     5521 tccaggacca cctccacgta gcgctccagc gacaggttgc gctcgctcgc cagccatgcc
     5581 tcgatctgcg cctgcaggtc ctcccgcggg tccgacgtcc accactgccc aagcacgcgc
     5641 tcagattcca cgcagcggta gaggtgctcg gtgaagccga tctcggtcac cccgcgggcc
     5701 agggcggcct ccacgtagcg gtcgatgtag ccgggcggat agacgcccat gggcggcgcg
     5761 cccggcgtgg gccgatgcgg gtggaggtgg aggtggtagt cgctcacgcg gcgaccgacg
     5821 tggcccggct caggatccgt gcgtccggaa gggagcgctc agctggtccc acagggcccc
     5881 ggcgttgttg ctctggatgt accccatcat gtactcctgc ccgatgtcga ccgggtagcg
     5941 atccgcgaag ctgatccgct ggatcgtctc ctcgcgggac catcccttcg ccacggcgtc
     6001 cgcgacggcg gcgcgccatt cgagcaggac cgccttctgg gtggcgaggt aggcggtcgt
     6061 ggtcaccggc ccgtggcccg ggacgacgtg gtcgacgtcg agcagcgcga tccggtccag
     6121 cgcggccacc cactggtcga tgtcggaggt catgagccag gtctgcacct cgctgaagac
     6181 ggtgtcgccg gtgaagacca cgcgctcctc gggaacgtgc accgcgagct gccccggcgt
     6241 gtggccggga gtgtggatca gctcgaaggt gtggtcgccg accctgagcg tcaggtcgcc
     6301 ggtgaagacg atccggccct tgttcgggtc ggcgtagtac acctcgcgct ccgggaagat
     6361 ggcctggccc tgcgggtcgt cggtggggat cgcctcggcg gcgtacgcga aggggtcgag
     6421 gatcggcgcg gggaccatga acagctcagc cacgccacgg tggtggacga ccctgcccgc
     6481 gcccttgaag tagtagttgc cgaagatgtg gtcgacgtgg tgctcggtgt tgaccacgta
     6541 ccggatcggc ccgtgcgacg ccgcctcctc gcgcatctcc acggcgcggg tcggcagctg
     6601 tggcgtgtcg atgaccacca cgccgtccgc ggtcgtcacg tagctcgggt tgcagccgcg
     6661 gatgtccgtc gcggtcctga cgttcggggt gacctgctgc atcggtgcgg cgccgcctac
     6721 tgggtggccg tcaggccgcc gtcgatcgtc agcacctggc cggtgacgaa ggcggacgca
     6781 tcggagcaga agaagaggac ggcgccgacc aggtcatcgg tggccgccac ccggcccagc
     6841 gggatgcggg caagcagccc gcgcttgaac tcctcgtcgg ccaggaggct ctcgacctgc
     6901 ggcgtggcga cgaaggtcgg ggcgatcgcg ttcacggtga tcgcgtgctt cgcccactcg
     6961 gtcgcctgct ggcgcgtgag cgcgttgatg gcgcccttgg ccgcgacgta ggcggagaac
     7021 ccctggttga tgcccagctg cccgcgcacc gacgagaccc ggagcacgcg gccgccgttg
     7081 ccggcggcaa tcatggcgcg cacggccgcc tgcgtcggca gcaggtcgct gcgcaggttc
     7141 agatcgagga tccggttcca ctccgactcg gggtagtcct cggccggata gagcgcggtg
     7201 ccggcaccgc cgccgacggc gttgacgatg atgtcgaccc ggccgaaggc ctccaggacg
     7261 cgatcgatgg cgcggtccgc gtcggccttt cgagtgacgt cggccgagac cagcagcgcc
     7321 tcggagccga tggcctcgat ctcgccggcg agctcggtcg aagcctccgc cgaacgaccc
     7381 aggaccgcga cccgggcgcc gacgcccgcg agggcgatcg acacgcgccg cccgatccct
     7441 ccggtcccgc cggacaccac ggccacacga ccatcgaggc ggaatcgggc gagctcgtcg
     7501 gccatgtcga aggcatctcc agcgggtgcg g
//
LOCUS       DASTYP010000149         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1613841, whole genome shotgun sequence.
ACCESSION   DASTYP010000149 DASTYP010000000
VERSION     DASTYP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613841"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFV59_06860"
     CDS             complement(<1..1067)
                     /locus_tag="VFV59_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5325586.1"
                     /translation="MIEAVAAPTKLLINGELRDAADAVTFTTRDPARGVELAEVARGS
                     AADVDAAVAAARGAFEGAWAKTSPAKRARALNRLAQLLDEQTDALADLESRNCGKAKW
                     QCAAEIKVAVSTLELFAGAAQHLYGRSHVVNPAVVSYTLREPVGVVGAIIPWNYPLMM
                     AAWKVAPALATGNTMVLKPASATPLTALRLGELALEAGIPAGVLNVVPGPGNEVGEAI
                     AAHPGIDKVAFTGETATGRRIAEVAAQSLKRVSLELGGKSPNIVFADADLDAAVLGSV
                     WAIYYAAGQSCEARSRILVQDEAYDAFASAFAEKTRSLAVGDPLDPDTKVGSLISRAH
                     AERVRGFIQTGLSDGGELLAGG"
     gene            complement(1093..1503)
                     /locus_tag="VFV59_06865"
     CDS             complement(1093..1503)
                     /locus_tag="VFV59_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325587.1"
                     /translation="MARMLALLLLATVVTACVVPAGAEVDTCGAEWRGIEAVIVQRGD
                     AALEPVSADCIRQVDERRIRIGFTMPGGPSCYRLADYAVEESADAVSVALFVARVDDP
                     AAGACAPEPRQVTTEIDLQAPVADRTLLDANGED"
     gene            complement(1549..>2591)
                     /locus_tag="VFV59_06870"
     CDS             complement(1549..>2591)
                     /locus_tag="VFV59_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5325588.1"
                     /translation="VAPDGTVRFAVGDVPAGVQTESLTRELSSAAGGGALEIEVPSAR
                     AGFSGAFTVALVVTGIAVVIALLLAATILSDRLTRPLRAVAQAAHRLGAGDLSARAEG
                     GPDAESAELATAFNEMATRLERSEALRRRAASDLAHDLATPATVLESQIQAMVDGVVP
                     ADAAELEKARAAAASLSSVIGQLGELTQAESAPLQRQPERVELAALARDAVAALDGML
                     RDRQVHASVDGSNAAAVTVDAGQVARALRNVLANAAQHSPVGGVVQVIVGDDASIRVV
                     DHGPGIAPEDLPFVFERFYRADRSRGGQRPGSGIGLTVARELVAANGGSIEVERTGPD
                     GTTFLLRLPPAA"
BASE COUNT          350 a          977 c          921 g          343 t
ORIGIN      
        1 ccgccggcga gcagctcgcc gccgtcgctg agcccggtct ggatgaagcc gcgaacgcgc
       61 tcggcgtgcg cgcgcgagat caacgacccg accttcgtgt ccgggtccag tgggtctccc
      121 accgcgagcg atcgtgtctt ctccgcgaag gccgatgcga acgcgtcgta cgcctcgtcc
      181 tggaccagga tccgcgaccg ggcctcgcac gactgtccgg ccgcgtagta gatggcccag
      241 accgatccga gcacggccgc atccaggtcc gcatcggcga agacgatgtt cgggctcttg
      301 ccgcccagct cgagcgagac gcgcttgagg gactgggccg ccacctcggc gatgcgccgc
      361 cccgtggccg tctcgccggt gaaggcgacc ttgtcgatgc cggggtgcgc ggcgatcgcc
      421 tcgcccacct cgttcccggg ccccgggacg acgttcagga cgccggccgg gatgccggcc
      481 tcgagcgcca gctcgcccag gcgaagagcc gtcagtggag tcgcggaggc cggcttgagg
      541 accatggtgt tgccggtggc cagcgcgggg gctaccttcc aggcggccat catcagcggg
      601 tagttccacg ggatgatggc gccgaccacg ccgaccggct cgcgcagcgt gtacgagacg
      661 accgccgggt tgacgacgtg cgatcgaccg tacaggtgct gcgccgcgcc ggcgaacagc
      721 tccagcgtgc tgaccgcgac cttgatctcg gccgcgcact gccacttcgc cttgccgcag
      781 ttgcgcgact cgaggtcggc aagcgcatcg gtctgctcgt ccagcagctg cgccagccgg
      841 ttgagcgccc gcgcccgctt ggccggcgag gtcttcgccc aggcgccctc gaacgctccg
      901 cgcgcggcgg cgaccgccgc gtcgacgtcg gccgccgatc cccgggcgac ctccgccagc
      961 tcgacacccc gtgccgggtc ccgggtggtg aaggtcacgg cgtccgcggc atcgcggagc
     1021 tcgccgttga tgagcagctt ggtgggggcg gcgacggctt cgatcatgcg cggcatggta
     1081 ccccgctcgc cgtcagtcct cgccgttggc atccagcagg gtgcggtctg cgacgggcgc
     1141 ctggaggtcg atctcggtcg tcacctgccg cggctccggc gcgcacgccc ctgccgccgg
     1201 atcatcgacc cgggcgacga acagcgccac cgacacggcg tcggcgctct cctccacggc
     1261 gtaatcggcc agccggtagc acgacggacc acccggcatg gtgaagccga tgcgaatgcg
     1321 tcgctcgtcc acctgccgaa tgcagtcggc gctgaccggc tccaacgcgg catccccgcg
     1381 ctgaacgatc accgcctcga ttccgcgcca ctccgcgccg caggtgtcca cctcggctcc
     1441 cgccggcacg acgcacgcgg tgacgaccgt ggcgagcagc aggagcgcga gcatccgggc
     1501 catcggcgcc atcatgaccg atgcgcctgc cggcgcgcga cggccgcctc aggcggccgg
     1561 gggcaaccgg agcaggaacg tcgtcccgtc gggacccgtc cgctcgacct cgatcgagcc
     1621 gccgttcgct gccacgagct cacgcgcgac cgtcagcccg atgccgctgc cgggtcgctg
     1681 accaccccgc gaccgatccg ctcggtagaa ccgctcgaag acgaacggca ggtcctcggg
     1741 cgcgatcccg ggtccgtggt ccacgacccg gatcgacgcg tcgtcgccga cgatgacctg
     1801 caccactcca ccgacgggcg aatgctgggc cgcgttggcc aggacgttgc ggagtgctcg
     1861 cgccacctgg ccggcgtcta cggtcacggc ggccgcgttc gagccgtcga cgctggcatg
     1921 gacctggcgg tcgcgcagca tgccgtcgag ggcggcgacc gcgtcgcggg ccagggccgc
     1981 aagctccact cgctcgggct ggcgctggag cggcgccgac tcggcctgcg tcagctcgcc
     2041 gagctgcccg atgacgctgg agaggctggc cgccgcggcg cgggccttct cgagctcggc
     2101 ggcatcggcc gggacgacgc cgtcgaccat cgcctggatc tgcgactcga ggacggtcgc
     2161 gggcgtcgcg aggtcgtggg ccaggtcact tgccgcgcgg cggcgaaggg cctccgaccg
     2221 ctccagccgc gtcgccatct cgttgaaggc cgtcgccagc tcggccgact ccgcgtccgg
     2281 accgccttcg gctcgcgcgc tgaggtcccc ggctcccagc cgatgggccg cctgcgccac
     2341 ggcgcgcagc ggccgggtga gccggtccga gaggatggtg gcggccagca gcagggcgat
     2401 gaccacggcg atcccggtca cgaccagcgc gacggtgaag gcccccgaga agccggcccg
     2461 agcgctgggc acctcgatct cgagcgcgcc gcccccggcg gcggacgaga gttcacgcgt
     2521 gagcgattcg gtctggacac ccgccggaac atcgccgacg gcgaaccgaa ccgtgccgtc
     2581 cggcgcgacc a
//
LOCUS       DASTYP010000150         9196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1359478, whole genome shotgun sequence.
ACCESSION   DASTYP010000150 DASTYP010000000
VERSION     DASTYP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359478"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFV59_06875"
     CDS             complement(<1..980)
                     /locus_tag="VFV59_06875"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU5325589.1"
                     /translation="MSVIAVVGAQWGDEGKGRVVDYLADEADLVIRYGGGNNAGHTVI
                     NPHGHFKLHLIPSGVFHPGTRNLLGNGVVINPPALVKELDGLADAGFAGDNLFISDRA
                     HLVMPYHVLLDQLDERERGASKLGTTSRGIGPAYIDKVARRGLRVVDLMDADTFRSRL
                     DATLPRIRRLVAGYGADDWADEIATATDPDRIADEYLAAAERLRRHVVDGQALVDEAL
                     ERGDRILLEGQLGTMRDIDWGIYPYVTSSSPIPGGASLGAGLPAVRIDRVVGVAKAYT
                     TAVGAGPLPTELDDEDGTQLRERGAEYGTSTGRPRRCGWYDAVAVRFSIRL"
     gene            complement(1032..2660)
                     /locus_tag="VFV59_06880"
     CDS             complement(1032..2660)
                     /locus_tag="VFV59_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEU5325590.1"
                     /translation="MSARVLRVLIALVLGTTILATAPAAPTRAGTMAVERLAGADRFQ
                     TAAAISARHYAPGVPAAYVATGLGFPDALAGGPAAVTAGAPILLVTPGGIPAATAAEL
                     ARLQPGRIVVLGGPGVVSDGVLAQLDAYDTGGGVERIGGADRFETAALVSARHFGPGT
                     ATAYVATGANFPDALVGGVAAARAGSPVLLTTPSAVPPATDAELRRLAPQRIVVLGGP
                     GVVPDAIAAQLGTIAPVSRLAGATRYDTGVAVSAASFASPAAGLFVATGATFPDALVA
                     VPAAAISGAALLLVPGTSAPASVQAEAARLAPGRLVLLGGPAVVNDRVTYQVRQATGD
                     LAPLPACSYQDVATPFPSLADWERTQVDTILMVGPEYYPGDLVDTSAAGLNGGHAVRG
                     HVVPDLAAMAAAARATGVPIQVQSAFRDYATQQATFDYWVSVGGYDQALRTSARAGHS
                     EHQLGITFDFTSAGGAAPWNYADWATTPAGAWMAANAWRYGFAMSYPRNAFATVCYDY
                     EPWHYRYVGRDAAWRIWISGLTVREWLWLNGNGG"
     gene            complement(2814..3119)
                     /locus_tag="VFV59_06885"
     CDS             complement(2814..3119)
                     /locus_tag="VFV59_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEU5325591.1"
                     /translation="MYADKTIRCRDCGMDFVFSAGEQEFYASKGLMNEPQRCQSCRAI
                     AKQNRALGISTGNNSGQREMHAAVCAECGGQALVPFLPRNDRPVYCSNCFDKVRART"
     gene            complement(3307..4653)
                     /gene="dnaB"
                     /locus_tag="VFV59_06890"
     CDS             complement(3307..4653)
                     /gene="dnaB"
                     /locus_tag="VFV59_06890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5325592.1"
                     /translation="MTLDKLAPQAVEAEQSVLGSILIDADAILKVGDFLKPHDFYRSQ
                     HGDIYEAMLALHGQREPIDLVTLGDELRRRDRLDAIGGPSYLATLMNAVPTAVHAEHY
                     GRIVERKAVLRNLIAAAGRIAAVGYEEANDAEVAIDRAESILFEISQRRTDGGFDSLS
                     TLLGQAYDRLEYLHEHRGQILGVPTGLSQLDALLGGFQPSDLVILAARPSVGKTSMAL
                     NLAQHAAVREGKKVAVFSLEMSKEQLALRLLSAETGINPRPLQTGFVDETDWSKIATV
                     MNDMASAPMWIDDSPALSVMELRTKARRLEAEQRGLDLVIVDYLQLMQASTPNKDANR
                     VQEVSEISRGLKQLARELKVPVIALSQLSRGVEQRGTAEPRLSDLRESGSIEQDADVV
                     IFLYREGEQNPDAEVELVKAKVAKHRNGPIGEVPLQFRKANTRFYTVAAREEIAAY"
     gene            complement(5026..5472)
                     /gene="rplI"
                     /locus_tag="VFV59_06895"
     CDS             complement(5026..5472)
                     /gene="rplI"
                     /locus_tag="VFV59_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5325593.1"
                     /translation="MKVILKHDVKGLGREGDMKEVKDGYARNFLLPTGAAMIADKGAV
                     ANWERHREQREERDRTLRAEAEATAEKLNQLRIEIPVKAGERGRLFGAVTSHQIVEAI
                     RREGVEIDRHALHLREPIKTLGDYKVDARVMAGVDATVSVHVVPQE"
     gene            complement(5469..5957)
                     /locus_tag="VFV59_06900"
     CDS             complement(5469..5957)
                     /locus_tag="VFV59_06900"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEU5325594.1"
                     /translation="MNLTTPARYLRMARLVVKLPTYARLVWGLVRDPRTPVGLKAMLG
                     AALAYVVFPIDLIPDAIPIIGQADDLTVLLLVLDLFIQNAPPEVRAEHTARARNGTAD
                     LDRDLARLRGLMGDRFDRIRDNLPELLDRYGQLRDPDELKRALSAWRRQRSSQTPPKE
                     LR"
     gene            6088..6741
                     /gene="lexA"
                     /locus_tag="VFV59_06905"
     CDS             6088..6741
                     /gene="lexA"
                     /locus_tag="VFV59_06905"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU5325595.1"
                     /translation="MTRHDAERRQRILDCIARTVAERGYPPSVREIADAVGLASTSAV
                     HHHLIALEKDGLLERGTHASRALRLTPRQSRPADAGKVTPFRMRVERETLRLPVMGEI
                     AAGQPIEAYADAAETLDVPASLEAREDAYVLRVRGKSMIDALIDDGDFVIVQPQATAR
                     DGDIVVALLEDNGVTLKRFFREKDRIRLQPANAEMEPIYATDVQIQGKVVGVIRRLG"
     gene            6752..7945
                     /locus_tag="VFV59_06910"
     CDS             6752..7945
                     /locus_tag="VFV59_06910"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5325596.1"
                     /translation="MPAAAPLSTRDLNRATLARQGLLEPIVVGPGDAVRRLGSLQAQH
                     PEWPPVALAARSADAATADLRGALDRREVVRSSLMRITIHVVAADDLWPMFAVCQPLR
                     LAQWSLLTKVDPLDSDLGERLASAHAIAIAALRERPRSSLELDRLMSHQAGLDPDALT
                     RPAWREPATRIPVRVAWRHFAAFVPLVHVPHDGEGYGRSQYALAADWLGQDDPEVPIE
                     EARARLARRFLAAFGPATLDDLAAYVGRGRGGLRVWRDALAALDDELVVLRDDDGPTL
                     FDLRDAPRPGGDVDAPPRLLARWDSLLLSHAASARQRIIADRDRPAVFSKNADVLPTF
                     TIDGFVAGTWQLSRSGGEASVQLQPFRALSRAVGEALVSEAERVLALLEPEAARRVHL
                     GPPAD"
     gene            7977..>9196
                     /locus_tag="VFV59_06915"
     CDS             7977..>9196
                     /locus_tag="VFV59_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5325597.1"
                     /translation="MQICPSCGEENPPKFRLCGYCGTPLAPAVTPTEERRTVTIVFSD
                     LQGSTKLGEALDPESVRAVMTRYFDSMTAVLRRHGGTIEKFIGDAIMAVFGLPRVHED
                     DALRAVRAARETQDALAQLNEELERVYGIRLTNRTGVNTGEVVTGDAATQQRLVTGDT
                     VNTAARLEQAAGPNEVLMGELTLQLVRDAVNVEPVEPLELKGKAERVPAYRLREVHAG
                     ADGLARRGDQPLVGRAAELGALHEVFDDAVLARRARLATVIGDAGVGKSRLIREVVDR
                     LSDRATVVRGRCLSYGEGITFWPVVEAVRDAADIGVDDPADVGHGKLATLVSDPAVSD
                     RLASLLGFHDETYSVDELSWAIRRWLEIVAEANGAVVWVIDDIHWAEATLLTLLQQLT
                     ETVADRSILLLCSSR"
BASE COUNT         1270 a         3279 c         3300 g         1347 t
ORIGIN      
        1 aggcgaatgc tgaagcgcac cgcgacggcg tcgtaccagc cacagcggcg cgggcggccg
       61 gtcgaggtgc cgtactcggc gccccgctcg cgcagctgcg tgccgtcctc gtcgtccagc
      121 tccgtcggca gcggcccggc gccgacggcc gtggtgtagg ccttggcgac ccccaccacc
      181 cggtcgatgc gcaccgccgg cagcccggcc ccaaggctcg cgccgccggg gatcggcgag
      241 gagctcgtca cgtacgggta gatgccccag tcgatgtctc gcatcgtgcc gagctgcccc
      301 tcgagcagga tgcggtcgcc ccgctcgagc gcctcgtcga ccagcgcctg tccgtccacc
      361 acgtgccggc gcagccgttc cgccgctgcg aggtactcat ccgcaatccg atccggatcc
      421 gtcgccgtgg cgatctcatc ggcccagtca tcggcgccgt agccggcgac cagccggcga
      481 atgcgcggca gggtggcgtc gagccgcgaa cggaaggtgt ccgcgtccat gaggtcgacg
      541 acccgcaggc ctcgccgagc caccttgtcg atgtaggccg gaccgatgcc ccgcgacgtc
      601 gtccccagct tcgaggcgcc gcgctcgcgc tcgtcaagct gatcgagcag cacgtggtag
      661 ggcatgacga ggtgggcgcg gtcgctgatg aacaggttgt cgccggcgaa gccggcatcg
      721 gccaggccgt cgagctcctt caccagcgcc ggcggattga tgaccacgcc gttgcccagc
      781 aggttgcgcg tcccggggtg gaagacgccg gacgggatca ggtggagctt gaagtggccg
      841 tgcgggttga tgaccgtgtg gcccgcgttg ttgccgccgc cgtagcggat gacgaggtcc
      901 gcctcatcgg cgaggtagtc gacgacccga cccttcccct cgtcgcccca ctgggcaccg
      961 acgacggcga tgacggacat ggcgggacgg cggcctcgag ctgctgtggt ggaggtcgcc
     1021 aatgctaccc atcagccacc gttgccgttg agccagagcc actcgcggac cgtcaacccg
     1081 gagatccaga tgcgccatgc ggcgtcgcgg ccgacgtacc ggtagtgcca cggctcgtag
     1141 tcgtagcaga cggtggcgaa ggcgttgcgt ggatagctca tcgcgaaccc gtagcgccac
     1201 gcgttcgcgg ccatccacgc gcccgcgggc gtcgtcgccc aatcggcgta gttccacggc
     1261 gcggcgccac cagccgaggt gaagtcgaag gtgatcccga gctggtgctc cgagtgacca
     1321 gctcgtgcgc tggtccgcag cgcctggtcg tagccgccga cgctgaccca gtagtcgaac
     1381 gtcgcctgct gcgtggcgta gtcgcggaag gcgctctgca cctggatcgg cacgccggtc
     1441 gctcgcgccg ccgccgccat tgcggccagg tcgggcacca cgtggccgcg caccgcgtgt
     1501 ccaccgttga gcccggccgc ggacgtgtcg accaggtcgc cggggtagta ctccggcccg
     1561 accatcagga tcgtgtcgac ctgcgtgcgc tcccagtcgg cgagcgacgg aaacggcgtc
     1621 gccacgtcct ggtaggagca cgcgggcagc ggagcaagat cgccggtcgc ctggcgcacc
     1681 tggtaggtca cccgatcgtt gacgacggcc gggccgccca ggaggaccaa ccgaccgggg
     1741 gcgagccgcg ccgcctccgc ctgcaccgat gcgggcgccg acgtgcccgg gacgagcagc
     1801 agggccgcgc cgctgatggc ggcggccgga acggcgacca gcgcgtcggg gaacgttgcg
     1861 ccggtcgcga cgaacaggcc ggccgcgggt gatgcgaacg acgcggcgct gaccgcgacg
     1921 ccggtgtcgt accgcgtggc accggccagg cgtgacacgg gggcgatcgt cccgagctgc
     1981 gccgcgatgg cgtccgggac gacgcccggt ccgcccagca ccacgatccg ctgcggtgcc
     2041 aggcggcgaa gctctgcatc ggtcgcaggc gggaccgcgg atggggtcgt gagcagaacg
     2101 gggctccccg cgcgcgcggc cgcgacgccg ccgaccagcg cgtccgggaa gttcgcgccg
     2161 gtcgccacgt aggcggtggc ggtgcctggg ccgaagtgcc gggcgctgac cagcgcggcc
     2221 gtctcgaacc gatccgcccc accgatgcgc tcgacgccgc cgcccgtgtc gtacgcgtcc
     2281 agctgcgcca gcacgccgtc ggacacgacg cccgggccgc cgaggaccac gatccgcccg
     2341 ggctgtagcc gcgccagctc ggcggccgtg gcggcgggga tgccgccggg cgtgaccagg
     2401 aggatgggag cgcccgcggt gacggcggcg ggtccgccgg cgagggcgtc ggggaacccg
     2461 aggcccgtgg cgacgtaggc ggcgggaaca cccggcgcgt agtggcgtgc gctgatcgcg
     2521 gcggctgtct ggaaccggtc ggcaccggcc agtcgctcga cggccatggt cccggcgcgc
     2581 gttggtgccg ccggggccgt ggcgaggatc gtcgttccca ggacgagggc gatcaggacg
     2641 cggaggacgc gcgcggacat cggggctcca gctccgtttg tgacgtgtgg tgaccacgat
     2701 catcgggagc cgcggcgcgg cccggcatcg gccaatcgtc gcgagcgcgc atgagacgaa
     2761 gaggcgccgc tgctggcggc gcctcggcgt ggagtttcgg tatgtgcgct ggatcaggtc
     2821 cgggcgcgca ccttgtcgaa gcagttggag cagtagaccg gtcgatcgtt cctgggcagg
     2881 aacggaacga gcgcctggcc cccacactcc gcgcacaccg cggcgtgcat ctcccgctga
     2941 ccgctgttgt ttccggtcga gatcccgagc gcacggttct gcttcgcgat ggcgcgacag
     3001 ctctggcagc gctggggctc gttcatcagg cccttgctgg cgtaaaactc ctgttcgcca
     3061 gcagagaaga cgaagtccat cccgcagtcg cgacagcgga tggtcttgtc ggcgtacacc
     3121 tgcctcacgt gcctcctgtt tcgcacgccc gggccctgga cgatcgcgtt cagcgagcgg
     3181 gccatgatct gcgtgcgaat gaggcgcgga tcgggacaga ctcgggtcaa cgctttgggc
     3241 cggccgttaa cgcgcccgag tgtacacccc gatttcaacc cttttgccgg gcttgcccct
     3301 gcggtttcag taggcggcga tctcctcccg tgcggcaacc gtgtagaagc gcgtgttcgc
     3361 cttccggaac tggagcggca cctcgccgat ggggccgttt cggtgcttcg ccaccttggc
     3421 cttgacgagc tccacctcgg cgtccgggtt ctgctcgccc tcccggtaca ggaagatcac
     3481 cacgtcggcg tcctgctcga tggatcccga ctcccgcagg tccgacagcc ggggctcggc
     3541 cgtcccccgc tgttcgacgc cgcgcgacag ctggctgagc gcaatgaccg gcaccttgag
     3601 ctcgcgcgcc agctgcttca ggccgcggct gatctcgctg acctcctgga cccggttggc
     3661 atccttgttc ggcgtcgacg cctgcatcag ctgcaggtag tcgacgatca cgaggtccag
     3721 cccgcgctgc tccgcctcca gccgtcgagc cttggtgcgc aactccatca ccgacagcgc
     3781 cggcgagtcg tcgatccaca tcggcgccga cgccatgtcg ttcatgaccg tggcgatctt
     3841 gctccagtcg gtctcgtcca cgaacccggt ctgcaggggc cgggggttga tgccggtctc
     3901 ggcgctcagc aggcgcagcg caagctgctc cttgctcatc tcgagcgaga agaccgcgac
     3961 cttcttcccc tcccgcacgg ccgcgtgctg ggccaggttg agcgccatcg acgtctttcc
     4021 gaccgaggga cgggcggcca ggatcacgag gtccgacggc tggaagccgc cgagcagggc
     4081 gtcgagctgg gagaggccgg tgggcacgcc caggatctgg ccacggtgct cgtgcaggta
     4141 ctccagccgg tcgtacgcct gcccgagcag cgtcgagagg gagtcgaagc cgccatcggt
     4201 ccgacgctgg ctgatctcga acaggatcga ctcggcgcgg tcgatggcga cctcggcgtc
     4261 gttggcctcc tcgtagccca ccgccgcgat ccgcccggcc gccgcgatca ggttgcgcag
     4321 caccgccttg cgctcgacga tccgcccgta gtgctcggca tggaccgcgg tcggcaccgc
     4381 gttcatcagc gtcgccaggt acgacggccc cccgatggcg tcgagccgat cccgacgccg
     4441 cagctcgtcg ccgagcgtga cgaggtcgat cggctcgcgc tgaccgtgga gcgccagcat
     4501 cgcctcgtag atgtcgccgt gctgcgagcg gtagaagtcg tgcggcttga ggaagtcgcc
     4561 gaccttcagg atcgcgtccg cgtcgatgag gatcgagccg aggacgctct gctcggcttc
     4621 gactgcctgg ggggcgagct tgtcgagggt catcggtcag cggttcgcaa gcagcggggc
     4681 acggcgcggc agactccgga ttcggacagg gaggacgccc ggccggtccg tggttggtga
     4741 ggaccgtcga gcaccgggcg caccgccatc gtcccggtct cgccgggctc gggcaaccgg
     4801 gttatcaacg aaggttgtcc acatccgccg tcgccgatgg tggatcgctc gtcgcggcat
     4861 gcggggagcc tgggggaccg tcgtgacagc tacggtgtca cgagcggcag gcggtcatcg
     4921 gggcgggtgg tcctgcggcg ggcaccgcga gtccgcgacc gccgcatccg gcgggcacgg
     4981 gaccgaagcc ggagggaccg acgcgtgccc gaaagcgagt gaggactact cctgcgggac
     5041 gacgtgcacg ctgacggtgg cgtcgacgcc cgccatcacc cgcgcatcga ccttgtagtc
     5101 gccgagggtc ttgatcggct cacggaggtg cagcgcgtga cggtcgatct cgacgccctc
     5161 gcgccggatg gcctcgacga tctggtggga ggtcacggcg ccgaagaggc gaccccgctc
     5221 gccggccttg accgggatct cgatgcgcag ctggttcagc ttctcggcgg tggcctccgc
     5281 ctccgcgcgc agcgtgcgat cgcgttcctc gcgctgctcc cggtggcgct cccagttcgc
     5341 gaccgcaccc ttgtcggcga tcatcgcggc gccggtcggc agcaggaagt tgcgcgcgta
     5401 gccgtccttg acctccttca tgtcgccctc gcgaccgagg cccttcacgt cgtgcttgag
     5461 gatgaccttc atcgaagctc cttcggtggt gtctgcgacg accgctgccg cctccaggcg
     5521 gacagggcgc gcttgagctc atcgggatcg cggagctgcc cgtatcggtc caggagctcc
     5581 ggcaggttgt cgcggatccg gtcgaagcga tcccccatca gcccgcgcag cctcgccagg
     5641 tcgcggtcca ggtccgccgt cccgttgcgt gcccgagcgg tgtgctcggc ccgcacctcg
     5701 ggcggcgcgt tctggatgaa caggtcgagc accagcagca ggaccgtcag gtcgtcggcc
     5761 tggccgatga tcgggatcgc gtcggggatc aggtcgatcg ggaacaccac gtacgcgagg
     5821 gcggcgccga gcatcgcctt caggcccacc ggcgtgcgcg gatcgcgcac gaggccccag
     5881 acgaggcgcg cgtacgtcgg cagcttgacg acgaggcgcg ccatgcgcag gtagcgggcg
     5941 ggcgtggtca ggttcaaggg gaggcctccg gccgtcgcga gtgggcaccg gataatagca
     6001 gagccgatgc gcctgcccgg tttgacatta gaacatgcgt tctgctacgg tgcgggcaac
     6061 acgcacgccc gagcgcggag gaggctcttg accaggcacg acgcggaacg ccgccagcgg
     6121 atcctggact gcatcgcgcg caccgtcgcc gagcgcggct acccgccgtc cgtccgcgag
     6181 atcgccgatg cggtcggcct cgcatccacc agcgcggtgc accaccacct catcgccctg
     6241 gagaaggacg ggctgctcga gcgcggcacc catgcctcac gggccctgcg cctcacgccg
     6301 cgccagtcgc gcccggccga tgcgggcaag gtcacgccct tccggatgcg ggtcgagcgc
     6361 gagacgctcc gcctcccggt catgggcgag atcgccgccg gccagcccat cgaggcctat
     6421 gccgatgcgg cggagacgct cgacgtgccc gcctcgctcg aggctcggga ggacgcctac
     6481 gtcctgcggg tccgcggcaa gagcatgatc gacgcgctca tcgacgacgg tgacttcgtc
     6541 atcgtccagc cgcaggccac cgcgcgtgac ggcgacatcg tcgtcgccct gctggaggac
     6601 aacggcgtca ccctgaagcg cttcttccgg gagaaggatc ggatccgcct ccagccggcg
     6661 aacgccgaga tggagccgat ctacgccacc gacgtgcaga tccaggggaa ggtcgtcggc
     6721 gtcatccgtc gactgggctg atccggccgc cgtgcccgcg gccgccccgc tgagcacgcg
     6781 ggacctcaac cgcgccacgc tcgcccgcca ggggctgctc gaacccatcg ttgtggggcc
     6841 cggggatgcg gttcgtcggc tcggctcgct ccaggcccag catcccgagt ggccaccggt
     6901 cgcgctcgcc gcgcggtccg ccgacgcggc gacggcggac ctgcggggcg cgctggatcg
     6961 ccgggaggtc gtccgcagct ccctgatgcg catcaccatc cacgtcgtcg ccgcggacga
     7021 cctgtggccg atgttcgcgg tgtgccagcc gctgcgcctg gcgcagtggt cgctgctcac
     7081 gaaggtcgat ccgctcgact ccgacctcgg cgagcgcctt gcgtccgcgc acgccatcgc
     7141 gatcgccgcc ctccgcgagc ggccgcggtc gtcgctcgag ctggatcggt tgatgagcca
     7201 ccaggccggc ctggaccctg acgcgctaac gcgtccggcg tggcgcgagc cggcaacccg
     7261 gatcccggtg cgcgtcgcgt ggcgccactt cgcggcgttc gtgccgctcg tccacgtccc
     7321 gcacgacggc gaggggtacg gccgatcgca gtacgcgctc gccgcggact ggctcggcca
     7381 ggatgacccc gaggtaccga tcgaggaggc acgagcccgg ctcgcgcgcc gcttcctggc
     7441 cgcgttcggt ccggccacgc tggacgacct ggccgcgtac gtcggccgcg gccgtggcgg
     7501 cctgcgcgtc tggcgcgatg cgctggcggc actcgatgac gagctggtcg tcctgcgcga
     7561 cgacgatggc ccgaccctgt tcgacctgcg ggacgcgccc cggcccggcg gcgacgtcga
     7621 cgcgccgccc cgtctgctgg ctcgctggga cagcctcctg ctgtcgcacg ccgcctccgc
     7681 ccggcagcgg atcatcgccg accgcgatcg gcccgcggtc ttcagcaaga acgccgacgt
     7741 gctgccgacc ttcaccatcg acggcttcgt cgccgggacg tggcagctga gccgcagcgg
     7801 cggcgaagca tcggtccagc ttcagccgtt ccgcgcgctg tctcgagccg tgggggaggc
     7861 gctggtgagc gaggcggaac gggtcctcgc cctcctcgag ccggaggcgg cccgccgcgt
     7921 gcacctgggg ccgccggccg actagcgctc gctcgccgcc tagaatcggg cgtcagatgc
     7981 agatctgccc gagctgcgga gaggagaacc cgcccaagtt ccgtctctgt ggatactgcg
     8041 gcacgcccct cgcgcccgcc gtcacgccga cggaggagcg ccgtacggtc acgatcgtct
     8101 tcagcgacct ccagggctcg acgaagctcg gcgaagcgct cgatcccgag tcggtccggg
     8161 cggtcatgac gcgctacttc gactcgatga cggccgtcct gcggcggcac ggcgggacca
     8221 tcgagaaatt catcggcgac gcgatcatgg ccgtcttcgg cctcccccgc gtccacgagg
     8281 acgatgccct tcgcgccgtg cgcgcggcgc gtgagaccca ggacgcgctg gcgcagctca
     8341 acgaggagct cgagcgcgtc tacggcatcc ggctcacgaa tcgaaccggc gtgaacaccg
     8401 gcgaggtcgt caccggcgac gccgcgacgc agcagcgtct cgtcaccggc gacacggtca
     8461 acacggccgc tcggctggag caggccgccg ggccgaacga ggtgctcatg ggcgagctga
     8521 cgctccagct cgtccgcgat gcggtcaacg tcgagccggt ggagccgctg gagctgaagg
     8581 ggaaggccga gcgcgtgccg gcgtaccgcc tccgcgaggt ccacgccggg gccgacggcc
     8641 tggcgcgccg cggtgatcag ccgctggtgg ggcgcgcggc cgagctcggc gcgctgcacg
     8701 aggtcttcga cgacgcggtc ctcgcgcgcc gcgcccgtct ggcgaccgtc atcggtgacg
     8761 ctggcgtggg caagtcgcgc ctgatccgcg aggtcgtcga tcggctcagc gaccgcgcca
     8821 ccgtcgtgcg tggccgctgc ctgtcgtacg gcgagggcat caccttctgg ccggtggtgg
     8881 aggccgtccg ggacgccgcc gacatcggcg tcgacgatcc ggcggacgtc ggtcacggca
     8941 agctggccac tctggtcagc gatccggcgg tgagcgaccg cctcgcgtcg ctgctcggct
     9001 tccacgacga gacctacagc gtcgacgagc tgagctgggc catccgtcgc tggctcgaga
     9061 tcgtggccga ggcgaacggg gcggtggtgt gggtcatcga cgacatccac tgggcggagg
     9121 cgaccctgct gaccctcctc cagcagctga ccgagaccgt cgccgaccgc tcgatcctgc
     9181 tgctgtgctc gtcgcg
//
LOCUS       DASTYP010000151         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_484415, whole genome shotgun sequence.
ACCESSION   DASTYP010000151 DASTYP010000000
VERSION     DASTYP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484415"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..988
                     /locus_tag="VFV59_06920"
     CDS             26..988
                     /locus_tag="VFV59_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5325598.1"
                     /translation="MSLVIDSITKRFGPILALDDVSFSVEPGRIFGLLGANGAGKTTS
                     MRIILDILRADHGRVTWRDTENIALPRRTWGYLPEERGLYPRMKVGEQLRFFASLFGV
                     SETDATAEIDDWLERFRIPEYRERRVEELSKGNQQKIQFIAAVLHDPEVLVMDEPFSG
                     LDPINVRMLKEAFTEMQARGKTVVFSTHQMEQVEELCEAIAIIDRGRVVVHGAVRDVK
                     RAMGRQVVRLATDGDGRGTDWLTSLPGVTITQDRADYVELRVPTGTDPGTILKAAVDR
                     GEHVTLFEIAEPSLEEVFVEHVGRRATDEADEHLAATQRTAAAR"
     gene            985..2307
                     /locus_tag="VFV59_06925"
     CDS             985..2307
                     /locus_tag="VFV59_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325599.1"
                     /translation="MNLFPNALHVARREYLFRVRGRAFKITTALIALAVAILTLLPVI
                     LAAIGVDEPPKIAVAVEAEDVTSDPIVAIQTVLLAAQDPQAVINGEEPQPIPEEDRAR
                     VTRTDDVDAARQQVRDGDLDGLLRIYRNGSGDLAFEYTTGAGPTNQVRLLVTQAASAL
                     AISDRLERAGISAGQSQELFAPPEFATVAANPDDARNEEDFGGAFMLAYAVVILTFMA
                     ILTYGQWVAQSVAEEKSGRVMELLITAATPRQLLTGKVLGTGAAGLTQYVVVIAAVIV
                     GLLANGPAAEALGVNTSSFPLPELEPGFVLIFGVFFLLGFVLYATLYAAAGSMVSRIE
                     DVQQAVGPLIFVAMAGYFASFAGLNAPDEPWVKIVSLVPFFSPYLMPARILLTTPSAA
                     EVLLAIALLAVTVVAAIWIASRIYSAGVLLYGQRVGIRSVLRATRVAR"
BASE COUNT          364 a          841 c          806 g          354 t
ORIGIN      
        1 tcacgaaccg agctgccgga gccccatgtc cctcgtcatc gactccatca ccaagcgctt
       61 cggcccgatc ctcgccctgg acgacgtctc gttctcggtc gagccggggc gcatcttcgg
      121 cctgctcggc gccaacggcg ccggcaagac gacgagcatg cggatcatcc tggacatcct
      181 tcgggccgac cacggccggg tgacctggcg cgacaccgag aacatcgcgc tcccccggcg
      241 gacgtggggc tacctgcccg aggagcgcgg cctctacccg cgcatgaagg tcggcgagca
      301 gctgcgcttc ttcgcctccc tgttcggcgt cagcgagacc gatgcgaccg cggagatcga
      361 cgactggctg gagcgattcc gcatccccga gtaccgcgag cgacgcgtcg aggagctctc
      421 gaagggcaac cagcagaaga tccagttcat cgcggcggtc ctgcacgacc cggaggtcct
      481 ggtcatggac gagcccttct cggggctcga ccccatcaac gtccggatgc tgaaggaggc
      541 cttcacggag atgcaggccc gaggcaagac cgtcgtcttc agcacgcacc agatggagca
      601 ggtcgaggag ctgtgcgagg cgatcgcgat catcgatcgc ggccgcgtcg tggtccacgg
      661 ggcggtccgg gacgtgaagc gcgcgatggg ccgccaggtc gtgcggctcg ccaccgacgg
      721 cgacggccgc gggaccgact ggctgacctc cctgccgggc gtgacgatca cccaggatcg
      781 cgcggactac gtcgaactgc gggtgccgac cgggacggac ccgggaacga tcctcaaggc
      841 cgcggtcgac cgcggcgagc acgtcacgct gttcgagatc gcggagccca gcctcgagga
      901 ggtcttcgtc gagcacgtcg ggcggcgagc gacggacgag gccgatgagc acctggccgc
      961 gacacagcga acggcggcgg cccgatgaac ctcttcccca acgccctcca cgtcgcgagg
     1021 cgcgagtacc tgttcagggt gcgcggccgc gccttcaaga tcaccaccgc gctcattgcg
     1081 ctggcggtcg cgatcctgac cctgctgccg gtgatcctgg ccgcgatcgg cgtcgacgag
     1141 ccgccgaaga tcgcggtggc ggtggaagcc gaggacgtca ccagcgatcc catcgtcgcg
     1201 atccagactg tcctcctggc ggcccaggat ccgcaggccg tcatcaacgg cgaggagccg
     1261 cagccgatcc cggaggagga ccgggcacgg gtcacgcgca cggacgacgt ggacgccgca
     1321 cgccagcagg tacgcgacgg tgatctcgat gggctgctca ggatctaccg caacggctcg
     1381 ggcgacctgg ccttcgagta caccacgggc gccggcccca ccaaccaggt gcgtctcctc
     1441 gtcacgcagg ccgcgagcgc cctggcgatc agcgatcgcc tggagcgggc agggatcagc
     1501 gccggccaga gccaggagct gttcgcgccg cccgagttcg cgacggtggc ggccaatccg
     1561 gacgacgccc gcaacgagga ggacttcggc ggagccttca tgctcgccta cgccgtcgtg
     1621 atcctgacct tcatggccat cctgacctac ggccaatggg tcgcgcagag cgtcgccgag
     1681 gagaagtcgg ggcgcgtcat ggagctgctg atcaccgccg ccaccccccg ccagctgctg
     1741 accggcaagg tgctgggaac cggagctgcc ggactgaccc agtacgtggt ggtcatcgcc
     1801 gccgtgatcg tcggcctcct ggcgaacggg ccggcggccg aggcgctcgg cgtgaacacg
     1861 tcatcgttcc cgctccccga gctggagccg ggcttcgtcc tgatcttcgg ggtcttcttc
     1921 ctgctcggct tcgtgctgta cgccacgctc tacgcggccg ccggctcgat ggtcagtcgc
     1981 atcgaggacg tccagcaggc ggtcgggccg ctgatcttcg tcgcgatggc gggctacttc
     2041 gcgtcgttcg cgggcctgaa cgcgccggac gagccatggg tcaagatcgt gagcctggtg
     2101 cccttcttca gcccgtacct gatgccggct cgcatcctcc tcacgacgcc atcggcggct
     2161 gaggtcctcc tggccatcgc gctgctggcg gtgacggtcg tggccgccat ctggatcgcc
     2221 tcgcgcatct acagcgccgg cgtcctgctc tacggccagc gggtcggcat ccgatcggtc
     2281 ctgcgcgcca cgcgcgtcgc gcgctgatgc cgtcgctcgt catcgccggg cgccggatcg
     2341 agctgaccga gggcgacatc accac
//
LOCUS       DASTYP010000152         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_812796, whole genome shotgun sequence.
ACCESSION   DASTYP010000152 DASTYP010000000
VERSION     DASTYP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812796"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1125)
                     /locus_tag="VFV59_06930"
     CDS             complement(160..1125)
                     /locus_tag="VFV59_06930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325600.1"
                     /translation="MDTIGQLGLTLRRPTLDDVETATRLFERVDLHDTGAVDTDADET
                     RDDWRTADLANDHWLVTDAAGGAVAYGEVQLRRRVHLEGWVAVDPAWRRRGIGTVLAD
                     RVEARATELLPRTVEGARVTLEGWVDTASQAGVGFVADRGMSPVRRFWRMRIDLADEP
                     PERSALPEGIDIRPFEVGQDERATWEASEDAFADHWGHVRAPFEEWIRRTQGDLFDPG
                     LWFLATEGDTIVGTALCSPWLGGGWVGSLGVRRRWRGRGIGEALLRRAFAEFHRRGRR
                     TVALGVDAESLTGATRLYERAGMRVDRVYELSIRVLRAGESLETA"
     gene            complement(1148..1351)
                     /locus_tag="VFV59_06935"
     CDS             complement(1148..1351)
                     /locus_tag="VFV59_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325601.1"
                     /translation="MVRDRGRRAVIAGISTVLWLGYMAVGLAVIDANLPTVGGEGPEG
                     VAAFVGLVWGVVTVGLVMWAASD"
     gene            complement(1377..2150)
                     /locus_tag="VFV59_06940"
     CDS             complement(1377..2150)
                     /locus_tag="VFV59_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU5325602.1"
                     /translation="MAQMRPHEEPTEAAAEYLMTIRYMHGEGQPVIAARLAERLGVSA
                     ATVSEMVTRLVREGMLSVDPSTRQLNLTDAGRGAAERTFRRHALAEWMLTEVVGLGWA
                     EADEEAHHLQHAFSDRVTDKIDELLGRPPTCPHGNPIPRDGRTPERPAGFPMSEAQEG
                     HDVTILRVTEEAEEDARLLTFLQENDVRPGNVFAVTEVAAHIATMTLRRADAAEPRHA
                     GREVTIGLAAAAKLRVLDGRADPSLFHRVPDRARLAAAR"
BASE COUNT          330 a          898 c          855 g          329 t
ORIGIN      
        1 acgcgaccag ggtggccggc ggaacctcct gcaccgcgac cttgatccag gcgaacgagg
       61 agccccagat caggccgagc agcacgaagg caccgggcag catccagcga ggtcgatcag
      121 ccaccgcgga aggctacacc gggcggcggg cgctcgccgt caggcggtct cgagcgactc
      181 gcccgcgcgc agcacgcgga tcgagagctc gtagacgcgg tcgacccgca tccccgctcg
      241 ttcgtacagc cgcgtggccc cggtcagcga ctccgcgtcg acgccgagcg cgacggtacg
      301 acgcccgcga cggtggaact cggcgaacgc tcggcgcagc agcgcctcgc cgatcccccg
      361 tccgcgccac cgtcggcgca ccccgagcga cccgacccag cctccgccca gccacgggct
      421 gcacagcgcc gtgccgacga tcgtgtcgcc ctcggtggcc aggaaccaca ggccgggatc
      481 gaacaggtcg ccctgggtcc ggcggatcca ctcctcgaat ggcgcgcgaa cgtggcccca
      541 gtggtcggcg aaggcgtcct ccgatgcctc ccacgtcgcc cgctcgtcct gacccacctc
      601 gaaggggcga atgtcgatgc cctcgggcag cgcggatcgc tccggcggct cgtccgccag
      661 gtcgatccgc atgcgccaga agcggcggac ggggctcatg ccgcggtccg ccacgaaccc
      721 gacaccagcc tgcgaggcgg tgtcgaccca cccctcgagc gtgaccctgg cgccctcgac
      781 ggttctcggg agcagctccg tcgcgcgggc ctcgacccgg tccgccagca ccgtgccgat
      841 gccccgccgg cgccacgcgg ggtccaccgc cacccaccct tcgaggtgca cccggcgtcg
      901 cagctgcact tcgccgtagg cgacggcccc gccggcggca tcggtcacga gccagtggtc
      961 gttggccaga tcggcggtgc gccagtcgtc ccgtgtctcg tcggcgtcgg tgtcgacggc
     1021 gccggtgtcg tgcaggtcga cgcgctcgaa gaggcgggtc gcggtctcga cgtcgtcgag
     1081 agtcgggcga cgaagggtca ggccgagctg accgatggtg tccacgcccg gacgctgagc
     1141 gggcggctca gtcggaggcg gcccacatga cgaggccgac ggtcacgacg ccccagacca
     1201 ggccgacgaa ggcggcgacg ccctccggtc cctcaccgcc gacggtcggc aggttcgcgt
     1261 cgatgacggc gaggcccacg gccatgtagc cgagccagag gacggtggag atgccggcga
     1321 tgacggcgcg ccggccgcgg tctcgaacca tgctcgcgga atcctagcag acgatctcag
     1381 cgcgccgccg ccagccgagc acggtcggga acgcggtgga acagcgacgg gtcggctcgg
     1441 ccgtcgagga cgcgcagctt cgccgcggcc gccagcccga tggtcacctc gcggccggcg
     1501 tggcgaggct cagctgcgtc cgcgcgtcgc agcgtcatgg tcgcgatgtg tgcggcgacc
     1561 tcggtcaccg cgaacacgtt cccgggccgc acgtcgttct cctggaggaa cgtcaggagc
     1621 cgagcgtcct cctccgcttc ttcggtcacc cgcaggatcg tgacgtcgtg gccctcctgg
     1681 gcttccgaca tcgggaagcc ggcgggccgt tccggcgtgc ggccgtcccg cgggatcggg
     1741 ttgccgtgcg ggcaggtcgg cggccgaccc agcagctcgt cgatcttgtc cgtgacgcgg
     1801 tcactgaagg cgtgctgcag gtggtgcgcc tcctcgtccg cctccgccca tcccagcccc
     1861 acgacctcgg tcagcatcca ctccgccagg gcgtggcggc ggaaggttcg ctcggccgcg
     1921 ccgcggccgg catcggtcag gttgagctgg cgggtgctgg gatcgacgct gagcatgccc
     1981 tcgcgaacca ggcgcgtgac catctcgctg acggtggcgg cgctcacgcc cagccgctca
     2041 gccagccgcg cggcgatgac cggctgcccc tcgccgtgca tgtaacggat cgtcatcagg
     2101 tactcggcgg cagcctcggt cggctcctcg tgtggtcgca tctgggccat gcgccgagtc
     2161 tagccccgag gcggtgcccg gtggctcgcg gtggggcgcg cctcagttgc tccccggcgg
     2221 aactaacgga caaggtggcc gtgctcgatg gtcgctggtt gcccggaggc gcaacgccag
     2281 gaacggggag ccggcgcgct cggccccttc ttcgccgtta gctccgttcc aggacaacgg
     2341 ccgcttcagg gacggttcac ggcggttgtg agcgaaccgc tcgtggcgtc ccaggtccat
     2401 cctgccttcg cc
//
LOCUS       DASTYP010000153         6684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1359663, whole genome shotgun sequence.
ACCESSION   DASTYP010000153 DASTYP010000000
VERSION     DASTYP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359663"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..231
                     /locus_tag="VFV59_06945"
                     /pseudo
     CDS             1..231
                     /locus_tag="VFV59_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
     gene            complement(892..1920)
                     /locus_tag="VFV59_06950"
     CDS             complement(892..1920)
                     /locus_tag="VFV59_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEU5325603.1"
                     /translation="MARSIPFLDADTIRRAVPIGDLLDAVEAAYRDVAAGRDRSPLRS
                     HVALDHGDLLLMPGVRTGGAGATVKLVTVMPGNASRGLPTVQAVVAWFDADTGTPLAL
                     LDGGAVTAMRTGAASGVATRLLARTDASTLAVLGSGGQAEWQVRAVVASRPITRVLVH
                     ARTAQSRDAFAARMGEALGLDVRAVDSAEEAVSAADVVCAATTSTRPVFDAAWIRPGT
                     HVNGIGAYRLDMVELPPELFARASLIGVDSREAIRAEAGDVMAAVGQGLVTEQQLTEI
                     GSVARDFAERREPDAITVFKSVGLAIQDVAAAELVVARVLGDDRGPTDAPRREHARDE
                     DSQGRPPH"
     gene            complement(1934..3166)
                     /locus_tag="VFV59_06955"
     CDS             complement(1934..3166)
                     /locus_tag="VFV59_06955"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325604.1"
                     /translation="MPIAPYLTLLRGNRDFARLYVAQLASFAGDWFATVALLGLALEL
                     SGSSAVASLVLVLQTGGFAAATPLAGMLADRLDRRRLMVAADLARIPIALSFLLVRGP
                     ETLSIAFVGVALLAVGAAVFEPTSSAALPNLVDEDDLATANVLIGSAWGTMLAVGAAL
                     GGLVAVTLGRDAAFIVNALSFAASAALIVGIHRPLQLAREGDDDQRVGAVAGVRQALN
                     FVSGHRRVLSLVLSKTSFGVGTGVVALLAVFGTEVYDGGDAGIGALFAARGLGALIGP
                     FAARRVMGTEDHALIRGIAVSFVLVAGCYAVFPTMPTLMLAALLVFGAHCGGGAQWTL
                     STLGLQRATPDAIRGRVFSLDYGMVTLTIAVSTLAAGWLADGIGPRPAVWVMAALILA
                     AGAAWVRLSWPLLRDPAA"
     gene            3228..3575
                     /locus_tag="VFV59_06960"
     CDS             3228..3575
                     /locus_tag="VFV59_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325605.1"
                     /translation="MIENVRDDLSHRADTVREEIDALLWLLRTAALGALVGALWVELR
                     KPPEQRTWHGKLLGVVPYDFRLPSLGSLRQAYWNPRSPKVFTDRPLGVGWAVNIPTLL
                     RRAGVRNQFTRAR"
     gene            3613..4062
                     /locus_tag="VFV59_06965"
     CDS             3613..4062
                     /locus_tag="VFV59_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325606.1"
                     /translation="MSWFPILLVTHIALAVSLLLPSVLLPFVLRRSSAEPNAATRVLM
                     AMQGTGTLVIATGLAITGGAMLWILGPELLTQPWLLTALTIYAINLVVAAVISRPNLR
                     RLIGLSRGNTDDATWRRRARQQRYIAYGMAGMIGAIGLLMSTKPDLW"
     gene            4305..5576
                     /gene="cysS"
                     /locus_tag="VFV59_06970"
     CDS             4305..5576
                     /gene="cysS"
                     /locus_tag="VFV59_06970"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5325607.1"
                     /translation="MTLRLHDARRGGLHPFEPVPGRDRVGMYVCGVTPYDTGHLGHAF
                     TYVSFDVLHRFLEYLGHDVVYVQNLTDVDDDMLRKARETGEDYLALGNRHVTTFLTEM
                     AALNWLPPDHYPRATEHIPQMLETIGRLVDAGLAYAAQGHVYFSIDAWPRYGELSHLS
                     RDEMLPIANDRGNVPDMAGKRQPLDFVLWQPSLPDEPAWESPWGAGRPGWHIECSAMA
                     TAYLGARFEIHGGGGDLLFPHHESELAQSEGAGAERPWVSFWTHAGMLRYEGEKMSKS
                     LGNLVLVRDLLRTYPGDAIRHYLVSQHYRSEVTFHEADLDASADAAARLRHATQRAEE
                     LSPAEPACADASALRRPVRELRERFLAAMNEDLDTPRAMPALDALADLALTAEDEGLA
                     AEAGWMVRELGARILGLRLASVHASQAIAAA"
     gene            5573..6142
                     /locus_tag="VFV59_06975"
     CDS             5573..6142
                     /locus_tag="VFV59_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325608.1"
                     /translation="MSDPAVPVTPSEDDLDDLVPLSVRLGEVVPPEDPEDWTRPLTWV
                     AALGMLAAPLFTLAWFILAPPASPDGSFAATAAVAAFLAGGAAATGATQLGVARAWTA
                     TLGAGLFGSLVVVILGVVMAGERQVGTASPTLAHAFVAAVAGLGGAGAAAAIAAVVAR
                     LTSRVVRFLPALFVGAVTAVLLVEPLLAG"
     gene            complement(6139..>6684)
                     /locus_tag="VFV59_06980"
     CDS             complement(6139..>6684)
                     /locus_tag="VFV59_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325609.1"
                     /translation="FELSGGQRQRVVIAAALALEPSLLVADEPVSMLDVSIRAEILNL
                     LGRLAHEQGIAIVMITHDLSTVAAYADRVAVMYLGRIVEHGSTRQVLTNPRHPYAQAL
                     LSVVPIPDPSQRRRPVILGGETPDPSRVPSGCRFHPRCPAAFDRCPLVDPPLFAVEPG
                     HAAACLLVEPTSGTDASRCSA"
BASE COUNT          975 a         2501 c         2363 g          845 t
ORIGIN      
        1 acctacgtca agaacgtgct gtcgcgcgag tggatcagct catggacgac cgagtccctg
       61 cgtgccgggg cactggccgt caagaactac gcctggtacc aggtcgtcca ctggcgcggc
      121 tacgtcaacg ccgccggcga gtgcttcgac gtcttcgaca cgacgcgcga ccagcactac
      181 gacccgtcgc gcccgaccta cgcctccatg gccgcggccg tggacgccac ctggggcacg
      241 ctcgcgacga agggcggccg cctcttcgcg acctactaca acgccggcgc cgcctacgag
      301 gcgtgcggga acaacgcgaa cggctggcag atgttccagt ggggcagcca ggcctgcggc
      361 ctggccggga tgagcgcggc gcagatcatg gcggtctact acgccggcgt cagcgtgagc
      421 gccgccccga ccccggcccc gccgccggcg acgccggcac ccacgccgac gccggtcccg
      481 actccggtcc cgaccccggt tccgacgccc gcgccgacgc ccgcgcccgg cacgacgccg
      541 ccgggcagca ccgccccagc gaccccggca cccaccgcga cgccggtccc gacgccgctc
      601 cccaccccgg ttccggtgcc cgtggccacg ccacccccac cggctgagat gccgggaggt
      661 ggacaggtcg gcctgaccgc accgcctcct cccccgccgc cggaccccga gcccatcgtc
      721 atcgccgcac ccgacgcggc ggccgacgcg gcggactccg cggcgacccc gacgccccgt
      781 cgctcgtcgg gtgaccgcat cgtcgagttc gagcgcgcgg tcgaccgggc ccgcgcccgg
      841 gccgagcgaa cgcccggcgg cggtgttctc accgccgaca ccgaaccgac gtcagtgcgg
      901 tgggcgaccc tgcgaatcct cgtcgcgcgc gtgctcgcgt cgtggagcat cggtgggccc
      961 gcggtcgtcg ccgaggaccc gggccacgac cagttcggcc gccgcgacgt cctggatggc
     1021 cagcccgacc gacttgaaga ccgtgatggc atcgggctcg cgccgctcgg cgaagtcgcg
     1081 ggccacgctt ccgatctcgg tcagctgctg ctccgtcact aggccctgtc cgaccgcggc
     1141 catcacgtcc cccgcctcgg cacgaatggc ctcgcgggag tcgacgccga tgagcgacgc
     1201 tcgcgcgaag agctcgggcg gcagctccac catgtcgagc cggtaggcgc cgatgccgtt
     1261 gacgtgggtc ccgggccgga tccacgctgc atcgaacacc ggcctggtcg aggtggtggc
     1321 ggcgcacacc acgtccgcgg cgctgacggc ctcttcggcc gagtcgaccg cccggacgtc
     1381 cagtcccagc gcctccccca tccgcgccgc gaaggcgtcc cgcgactggg cggtccgggc
     1441 gtggacgagg actcgggtga tgggccgcga ggcgacgacg gccctcacct gccactcggc
     1501 ctggcccccg gacccgagga cggcgagggt cgacgcatcg gtccgagcca gcagccgggt
     1561 cgcgacgccc gaggccgcgc cggtgcgcat ggcggtcacc gccccgccat ccaggagcgc
     1621 caggggcgtg ccggtatcgg cgtcgaacca ggcgaccacc gcctgcacgg tcggcaggcc
     1681 gcgcgacgcg ttgcccggca tcacggtgac cagcttgacg gtcgctccgg cgccgccggt
     1741 gcgcacgccg ggcatgagca gcaggtcgcc gtggtccagc gccacgtgcg agcgcagcgg
     1801 cgaccggtcg cggccggccg ccacgtcccg gtaggccgcc tccacggcgt ccagcaggtc
     1861 gccgatcggg acggcgcggc ggatggtatc ggcgtcgagg aaggggatgg agcgtgccat
     1921 ggttgcgccg agtctaggcg gctgggtcgc gcagcagtgg ccagctcagg cgcacccacg
     1981 cggctccggc ggccaggatc agtgcggcca tgacccacac cgccggccgg ggaccgatcc
     2041 cgtcggcgag ccagcctgcc gccagcgtcg agaccgcgat ggtcagggtg accatcccgt
     2101 aatccaggct gaacacccgg ccgcggatgg catcgggggt ggcgcgctgc aggccgagcg
     2161 tcgacagggt ccactgggca ccaccgccgc agtgcgcccc gaacaccagc agagcggcca
     2221 gcatcagggt gggcatggtg gggaagacgg cgtagcagcc ggcgacgagc acgaacgaga
     2281 cggcgattcc ccggatcagc gcgtgatcct cggtccccat cacccgacgg gcggcgaatg
     2341 gaccgatgag ggcgccgagc cctcgagcgg cgaacagcgc gccgatgccg gcatcgccgc
     2401 cgtcgtatac ctcggtcccg aagacggcga gcagcgccac cacgccggtg ccgacgccga
     2461 acgaggtctt ggacagcacc agcgacagga cgcgacggtg cccgctcacg aagttcagcg
     2521 cctggcggac gccggcgacg gctcccacgc gctgatcgtc gtcgccttcg cgtgcgagct
     2581 gcagcggccg gtggatgccc acgatgaggg cggcggacgc cgcgaagctg agcgcgttga
     2641 cgatgaaggc ggcatcccgc ccgagggtca ccgccaccag gccaccgagc gccgcgccga
     2701 cggccagcat ggtcccccat gccgacccga tcaggacgtt ggcggtggcc agatcgtcct
     2761 cgtccaccag gttgggaagc gcggccgagg acgtcggctc gaagacggcc gcgccaacgg
     2821 ccagcagcgc gacccccacg aacgcgatcg agagcgtctc cggcccgcgg accagcagga
     2881 aggacagcgc gatcgggatc cttgccaggt ccgcggccac catcagccgg cgccggtcga
     2941 gtcggtccgc cagcatcccg gccagcgggg tggcggccgc gaagccgccg gtctgcagga
     3001 ccaggacgag cgaggcgacg gcggacgacc cgctgagctc cagcgccaac ccgagcaggg
     3061 cgacggtggc gaaccagtcg ccggcgaagc tggcgagctg ggcgacgtag agccgcgcga
     3121 aatcgcggtt gccgcgcagc agcgtaaggt agggggcgat cggcatcggc gccggcatga
     3181 tggcacgcgg cacgtcgctt gcgacggtcc ggagccaagg aggacccatg atcgagaacg
     3241 ttcgcgacga cctctcccat cgcgccgata cggtgcgcga ggagatcgat gcgctgctgt
     3301 ggctactgcg gaccgccgcg ctgggtgccc tggtgggcgc cctctgggtg gagctgcgca
     3361 agccaccgga gcagcgcacg tggcacggca agctgctggg cgtcgttccc tacgacttcc
     3421 gcctgccttc gctcggcagt ctgcgccagg cctactggaa cccgcgctcg ccgaaggtct
     3481 tcaccgaccg gccgctgggg gtcggctggg ccgtgaacat cccgacgctc ctgcgacgag
     3541 ccggcgttcg caaccagttc acccgcgcgc ggtaaggcga cactcccctt tctcggctag
     3601 gatcggagcc cgatgagctg gttcccgatc ctgctggtca cccacatcgc gctggccgtg
     3661 tcgctgctgc tgccgagcgt gctgctgccg ttcgtgctgc gccgcagcag cgcggaaccg
     3721 aacgccgcga cgcgcgtgct gatggccatg cagggcaccg gaaccctcgt gatcgcgacc
     3781 ggcctggcga tcaccggcgg cgccatgctg tggatcctgg ggccggagct gctcacccag
     3841 ccgtggctcc tgaccgcgct gacgatctac gcgatcaacc tcgtggtagc cgcggtgatc
     3901 agccgcccga acctgcgccg cctcatcggg ctgagccgcg gcaacaccga cgacgcgacg
     3961 tggcggcgcc gcgcacgcca gcagcggtac atcgcctacg ggatggccgg gatgatcggc
     4021 gccatcgggc tgctgatgag cacgaagccc gacctgtggt gagccgggcg accgctcact
     4081 gagcggctag aagtgcggca acgggatcgg gcgacccatg acgaagaaga tgcccgccac
     4141 gatcaggaac agggcgatcg cgaagaacag caaccagcgc aggacttcca tgctcgggct
     4201 ccacgtatgg tcgtgtcggc agcagactcc cccgttcgac ggcgattcta tcggtccagt
     4261 ccagccatcg gcgccgatcc agcgaccgct ttagaatccg caccgtgacc ctccgcctgc
     4321 acgatgcccg ccgtggcggg ctgcacccct tcgagcccgt ccccggtcgc gaccgcgtcg
     4381 gcatgtacgt ctgcggcgtg acgccgtacg acaccggcca cctgggccac gccttcacct
     4441 acgtcagctt cgacgtgctg catcggttcc tggagtacct gggccatgac gtcgtgtacg
     4501 tccagaacct gaccgatgtc gacgacgaca tgctgcgcaa ggcgcgcgag acgggcgagg
     4561 actacctcgc cctggggaac cggcacgtga ccaccttcct gaccgaaatg gcggcgctca
     4621 actggctgcc cccggaccac taccccaggg cgaccgagca catcccccag atgctcgaga
     4681 cgatcggccg cctggtcgac gccggcctcg cctatgccgc gcaggggcac gtctacttca
     4741 gcatcgatgc gtggcctcgc tacggcgagc tgtcgcacct gtcacgagac gagatgctgc
     4801 cgatcgccaa cgaccgcggc aacgttcccg atatggccgg caagcgccag ccgctcgact
     4861 tcgtcctgtg gcagccctcg ctccccgacg agcccgcctg ggagtcgccg tggggcgccg
     4921 gacggcccgg atggcacatc gagtgctccg ccatggcgac cgcgtacctg ggcgcgcgct
     4981 tcgagatcca cggcggtggc ggcgacctgc tcttcccgca ccacgagtcg gagctcgccc
     5041 agtccgaggg tgccggcgcg gaacgcccgt gggtctcgtt ctggacccac gccggaatgc
     5101 tgcgctacga gggggagaag atgagcaagt cgctcggcaa cctggtgctc gtgcgcgacc
     5161 tgctgcgaac ctacccgggc gacgccatcc gccactatct cgtgagccag cactaccgtt
     5221 cggaggtcac cttccacgag gccgacctgg acgccagcgc cgatgccgcc gcgcgactcc
     5281 gacacgcgac ccagcgcgcc gaggagctct cccccgccga gcccgcgtgc gccgatgcca
     5341 gcgccctccg gcgcccggtg cgcgagctgc gggagcggtt cctggccgcg atgaacgagg
     5401 acctcgacac gccgcgcgcc atgccggcgc tggacgcgct ggccgacctg gcgctcaccg
     5461 ccgaggacga gggcctcgcc gccgaggcgg gctggatggt gcgggagctg ggggcccgga
     5521 tcctggggct gcggctggca tcggtccacg cgtcgcaggc gatcgcggcc gcgtgagcga
     5581 cccggccgtg cccgtcaccc cgtccgagga cgacctcgac gacctcgtcc cgttgagcgt
     5641 gcggctcggc gaggtcgttc caccggagga ccccgaggac tggacgcggc cgctgacctg
     5701 ggtcgcggcg ctcggcatgc tggccgcgcc gctcttcacg ctggcctggt tcatcctggc
     5761 gccccccgcc tcaccggatg ggtcgttcgc ggcgaccgcg gccgtcgccg ccttcctggc
     5821 cggtggcgcc gcggcgaccg gggccacgca gctcggcgtc gcgcgagcct ggaccgcgac
     5881 gctcggcgcc ggcctcttcg gatcgctggt cgtcgtcatc ctcggggtcg tgatggccgg
     5941 agagcgccag gtcggaacgg cctcgccgac actggcccac gccttcgtgg cggcggtcgc
     6001 cgggctcggg ggcgcgggcg ccgcggccgc gatcgccgcc gtcgtcgcgc gcctcacgtc
     6061 ccgcgtggtg cgcttcctgc cggccctctt cgtcggcgcc gtgacggccg tcctgctcgt
     6121 cgagcccctg ctcgcgggct aggccgaaca cctcgacgcg tccgtcccgg acgtcggctc
     6181 caccagcagg catgccgcgg cgtggcccgg ctcgaccgcg aacagcggcg gatcgacaag
     6241 gggacatcgg tcgaaggcgg ccgggcagcg cgggtggaag cggcagccgg agggcacgcg
     6301 ggaggggtcg ggcgtctcgc cgcccaggat gacgggccgc cggcgctggc tcggatccgg
     6361 gatcggcacc acggacaaca gggcctgcgc atacgggtgg cgaggattcg tcagcacctg
     6421 gcgcgtcgac ccgtgctcca cgatccggcc caggtacatg accgccaccc gatcggcgta
     6481 ggcggcgacg gtcgagaggt cgtgggtgat catgacgatg gcgatgccct gctcgtgggc
     6541 cagccgcccg agcaggttca ggatctcggc ccggatgctg acgtccagca tgctgaccgg
     6601 ctcgtccgcc acgagcagcg acggctcgag cgccagtgcc gccgcgatca cgacgcgctg
     6661 gcgctggccg cccgacagct cgaa
//
LOCUS       DASTYP010000154         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_412058, whole genome shotgun sequence.
ACCESSION   DASTYP010000154 DASTYP010000000
VERSION     DASTYP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412058"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="VFV59_06985"
     CDS             complement(<1..111)
                     /locus_tag="VFV59_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEU5325610.1"
                     /translation="MTTKSKVPYRCSFCGKSQEQVRKLIAGQGVYICDECI"
     gene            182..670
                     /locus_tag="VFV59_06990"
     CDS             182..670
                     /locus_tag="VFV59_06990"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5325611.1"
                     /translation="MPVLRLGRVDTVDGPMLVAETDAGVVAVARGGSEPALTESLERR
                     IADVHVVAGEVDTSWLVGLLDGDAVPLPEVDLRGLSAFDARVYRAVREIPRGETATYG
                     DVAGMVGAPLAARAVGGAMARCPLFPAVPCHRVVRAADGWSGWGGDVALKRRLLAAER
                     RA"
     gene            complement(1417..2082)
                     /gene="clpP"
                     /locus_tag="VFV59_06995"
     CDS             complement(1417..2082)
                     /gene="clpP"
                     /locus_tag="VFV59_06995"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEU5325612.1"
                     /translation="MLVPMVIESSARGERAFDIYSRLLKDRIIFLGDAIEDHVANLII
                     AQLLFLESEDPEKDIQLYINSPGGVVTSGLAIYDTMQYLRAPVSTICIGQAASMGAVL
                     LACGATGKRYALPNSRIMIHQGSGGFRGNTPDVEIQMREMMHLTDRLMQILADHTGQS
                     FEKVKRDSERDYFMSAEEAKAYGLIDEVFAGNTESLISMAHKAGGEVPTAEEAAEADK
                     AEK"
     gene            complement(2085..3479)
                     /gene="tig"
                     /locus_tag="VFV59_07000"
     CDS             complement(2085..3479)
                     /gene="tig"
                     /locus_tag="VFV59_07000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU5325613.1"
                     /translation="MVTVSKRPEPGSKMVLEIEVPPAEVDRHFATAYRHVAERTKVPG
                     FRPGKAPRTVIDRFVGRASVMAEVIDHLVSDAYDAALNQTDVIPVDHPEVDIDPNAVV
                     EGQTVTFTATVPVRPEVELGSYTDYPFGLEVPEVTDEQVDQVIEELREQQATLRPVDG
                     RAAQNGDIASVKFVGSIDGTPFEGGSADQLPLVIGENRMIPGWEDHLVGLEIGGTAQF
                     DITFPEDYRVEELRGKEAHFDVELLDLREKILPEVTDDFAKSVGDVQTVDELRTEIRD
                     AMEKRADAEARHMFGDRIIDYAVSNATVELPEVMVTNEVEIMRDELRSRLAQQRIGLD
                     QYLALAKQTPEELTVELREPATRRVKTLLVLSAVAEKEGVDATDEEIQAEIEEQLART
                     GDDAKVREYLTSARGRSYLRMTLRNRKLVDTLVERALGTDASQGEAPDAAPEPATTEP
                     TEPTESSEQPEESN"
     gene            complement(3545..3628)
                     /locus_tag="VFV59_07005"
     tRNA            complement(3545..3628)
                     /locus_tag="VFV59_07005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3593..3595),aa:Leu,seq:tag)
BASE COUNT          507 a         1241 c         1319 g          632 t
ORIGIN      
        1 gatgcactcg tcgcagatgt agacgccctg tccggcgatg agcttgcgga cctgctcctg
       61 gctcttgccg cagaacgagc agcgatacgg gactttcgac ttcgtcgtca tcgcgcccta
      121 gggtagcacg gggggtccaa ggccccggca tcccccgttc gaggccgtac catcggcgcc
      181 gatgcctgtg ctccgccttg gtcgggtcga caccgttgat ggaccgatgc tcgtcgccga
      241 gaccgatgcg ggagtcgtgg cggttgcgcg gggtggctcg gagcctgcgc tgaccgagtc
      301 gctggagcgt cggatcgcgg acgtccacgt cgtggccggc gaggtggaca cgtcctggct
      361 tgtcggcctg ctggatggcg acgccgtccc actgcccgag gtcgacctgc gcgggttgtc
      421 ggccttcgac gcgcgcgtct accgcgcggt gcgcgagatc ccgcgtggcg agaccgccac
      481 ctacggcgac gtggcgggca tggtgggcgc tcccctggcg gcgcgggccg tcggcggggc
      541 gatggcgcgc tgtcccctct tcccggccgt gccgtgccat cgcgtcgtgc gggcagcgga
      601 cggttggtcg ggctggggcg gtgacgtggc gctcaagcgc cggctgctgg cggccgagcg
      661 acgcgcgtga ggtcggccgc gctcggagtt gcgcgggagg caaggaattc acgagatgcg
      721 ccgatgtgcc gcctctccgc ggccctgggt tgcgcgtcac gcaagcgaat gccaggaacg
      781 agcctctgcg cggccgttag ttgccgcgca tgcaagcggg cggtcgtcgc gaagcccgac
      841 aagccggcgg tcgcagcggg gcgacgccag atagcccgac cgcgctcgga gttgcgcgtg
      901 aagcaactaa ttcccgagat gtgccgatgt gccgcctctc cgcgccgctg ggttgcgcgt
      961 cacgcaagca aatgccagga acgagcctcc gctcggccgt tagttgccgc aggtgaaagc
     1021 gggtggtcgc agtgcggcca gacaaggcgg tggtcgcaaa gcgcggcccg agttgtcatt
     1081 cgcctgtctg ggcgacgtta gccagcgtcg ctccgcctcg aaccgccagg tcggaccgat
     1141 tccggctcgt agcgtctccc gaacaggcgg ctgacgactc cagcagcgga gatcccgtct
     1201 agcgtcgcca ggacagaccg atgatgacgc cagcggcgga gatcccgtcg agcgtcgcca
     1261 ggacagaccg gtgacgacgc cagcggcgga gatcccgtct agcgtcgcca ggacagaccg
     1321 atgacgactc cagcaccgcc gcgctgcctg ccgtcgccac caggcacccc tgacgactcc
     1381 agcggcgagc gcggccgggc gcgccaacgc ccggtgctac ttctcggcct tgtccgcctc
     1441 cgcggcctct tcggccgtcg ggacctcgcc cccggccttg tgggccatcg agatgagcga
     1501 ctcggtgttg ccggcgaaga cctcgtcgat caggccgtag gccttggcct cctcggcgct
     1561 catgaagtag tcgcgctccg aatcgcgctt caccttctcg aagctctgcc cggtgtgatc
     1621 ggcgaggatc tgcatcaggc gatcggtcag gtgcatcatc tcgcgcatct ggatctcgac
     1681 gtccggggtg ttgccgcgga agccgcccga accctggtgg atcatgatcc ggctgttcgg
     1741 gagcgcgtag cgcttgcccg tggcgccgca ggccaacagc acggcaccca tggacgccgc
     1801 ctggccgatg cagatggtgc tcaccggggc gcgcaggtac tgcatggtgt cgtagatggc
     1861 gaggccgctg gtcacgacgc cgcccggcga gttgatgtag agctggatgt ccttctcggg
     1921 gtcctccgac tcgaggaaca gcagctgggc gatgatcagg ttggcgacgt gatcttcgat
     1981 cgcatcgccc aggaagatga tgcggtcctt gaggagccgc gagtagatgt cgaacgcgcg
     2041 ctcgccgcgg gcgctcgact cgatgaccat cgggacgagc atgctcagtt ggactcctcg
     2101 ggctgctccg acgactcggt cggttcggtc ggttcggtgg tggcgggttc gggggccgcg
     2161 tcaggcgcct ccccctggga cgcatcggtc ccgagggcgc gctcgaccag cgtgtcgacc
     2221 agtttacggt tgcgcagcgt catgcggagg tagctgcggc cgcgggcgga ggtgaggtac
     2281 tcgcggacct tggcgtcgtc gccggtgcgg gccagctgct cctcgatctc ggcctggatc
     2341 tcctcgtcgg tcgcgtccac gccctccttc tcggcaacgg ccgagaggac cagcagcgtc
     2401 ttcacccggc gggtggcggg ctcgcgcagc tcgacggtga gctcctcggg cgtctgcttc
     2461 gcgagcgcca ggtactgatc cagaccgatg cgctgctgcg ccagccggct gcgcagctcg
     2521 tcgcgcatga tctcgacctc gttggtgacc atcacctcgg gcagctcgac ggtcgcgttg
     2581 ctcaccgcgt aatcgatgat ccggtcgccg aacatgtggc gcgcctcggc atcggcccgc
     2641 ttctccatgg catcgcggat ctcggtgcgg agctcatcca cggtctggac gtcgccgacc
     2701 gacttcgcga agtcgtcggt cacctcgggc aggatcttct cccggaggtc gagcagctcg
     2761 acgtcgaagt gcgcctcctt gccccgcagc tcctcgaccc ggtagtcctc ggggaacgtg
     2821 atgtcgaact gcgccgtgcc gccgatctcc aggccgacca ggtggtcctc ccagccgggg
     2881 atcatgcggt tctcgccgat gaccagcggc agctggtcgg cgctcccacc ctcgaacggc
     2941 gtgccgtcga tgctgcccac gaacttgacg ctggcgatgt cgccgttctg cgccgcgcgg
     3001 ccgtcgacgg gacgcagcgt ggcctgctgc tcgcgcagct cctcgatgac ctggtcgacc
     3061 tgctcgtcgg tcacctcggg cacctcgagg ccaaacgggt agtcggtgta cgacccgagc
     3121 tccacctccg gacggaccgg cactgtggcg gtgaaggtga ccgtctggcc ctcgaccacc
     3181 gcgttgggat caatgtcgac ctcgggatgg tcgaccggga tcacgtcggt ctggttgagc
     3241 gcggcgtcgt aggcgtcgct gaccaggtgg tcgatgacct cggccatgac gctggcgcga
     3301 cccacgaacc gatcgatgac ggtgcgcggc gccttcccgg ggcggaagcc gggcaccttg
     3361 gtgcgctcgg ccacgtgacg gtaggcggtg gcgaagtgcc ggtcgacctc ggcgggcggg
     3421 acctcgatct cgaggaccat cttcgagccc ggttcgggtc gcttcgatac ggtgaccaag
     3481 tcagctcccg ttgctcattc gatgttcggc gccgcggatg gggtcgcgcg ggcgaggtgc
     3541 gatgtggtgg gcgaggcgag attcgaactc gcacgtcttg cgacaccggc tcctaaggcc
     3601 ggcgcgtctg ccgttccgcc actcgcccgc gtggaagatg gtgcggcggg gaccgggtga
     3661 gtctaccaac ggaccgatgc ggccgctccg cggccggac
//
LOCUS       DASTYP010000155         7057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_667693, whole genome shotgun sequence.
ACCESSION   DASTYP010000155 DASTYP010000000
VERSION     DASTYP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667693"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1857)
                     /gene="treZ"
                     /locus_tag="VFV59_07010"
     CDS             complement(121..1857)
                     /gene="treZ"
                     /locus_tag="VFV59_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEU5325614.1"
                     /translation="MTGTRPAVWAPRPERVELVLGAERHAMRRREDGWWHADVELPPG
                     TDYAFSLDGGEPVPDPRSPWQPSGVHGPSRTVDHDAFAWTDSGWQAPPLADAVIYEAH
                     VGTFSPDGTFDAAIERLPDLADLGVTHLELMPVVEFSGDRGWGYDGVDLFAPHHAYGG
                     PDGLKRLIDAAHAHGLAVLIDVVYNHLGPEGNYLSRFGPYFSHKYATPWGDAVNFDER
                     GSHEVRRFVVDNALLWLRDYHADGLRLDAMQAIFDMSAVQIGEEIATAVHRLGEELRR
                     RLVVTAESDLNDPRLVRRPARGGYGMDAAWADDVHHALHAALSGETNGYYVDFTDAAA
                     LPRALHDPYLYADRYSAHRGRRHGRAADDVPGDRFIACLQNHDQVGNRARGDRLAQVV
                     SPGRARIGAALLLTSPYVPLLFAGEEWGASTPFRYFTGHASPELAQAVREGRRNEFVH
                     FGWDPADVPDPQDPETFHDSKLRWDERREPEHAAMLEWYRSLIALRRELPELRDGDRS
                     AVGVDGRSDSLLTVRRGRVTVAANLGTGPAGVAASGDIRLAWPPELALDAGDGSAATV
                     PPDGVVVLVERR"
     gene            complement(1854..4481)
                     /gene="treY"
                     /locus_tag="VFV59_07015"
     CDS             complement(1854..4481)
                     /gene="treY"
                     /locus_tag="VFV59_07015"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEU5325615.1"
                     /translation="MRHRPRDVPTATYRLQLHPGFTLDDARDVVPYLASLGISHLYLS
                     PILQAVGGSTHGYDVVDHSRVNEELGGDAAFDRLTDALRAHGMGLVLDIVPNHMAIAE
                     ENRWWWDVLRHGPASAYAHFFDVDWDPPESRLRNVVLLPILADHYGRVLEAREIRVVR
                     REGTLLVEASERMLPTDPQSVGAIVSKAAEEAGSDELAFIGSALSDLPSSTDPDPEQV
                     ARRQRDADVLEARLGELASDPEVAGVLDAAVERLNADVDGLDAFLEGQNYRLARWRAA
                     SRDLGYRRFFDIDELIGLRQEDERVFAATHERILGWVRDGRVQGLRIDHPDGLRDPAG
                     YFGRLREAAPDAWIVAEKILEPGESLPADWPIDGTTGYRFANLATGILVDPAGEEPLS
                     AAWAEVADAGPNWDDIVEGSRKEVLAAVLGSDVNRLTDLLLAICEANRRYRDFTRHEV
                     HEALREVAAALPVYRTYVRAADGHVSSVDRDLIAGSVETAVTRRPDLDPDLFAFLGRI
                     LRLEEPGPLAAELAMRFQQLTPAAMAKGVEDTAFYRHHRLVALNEVGGDPGHFGTPPD
                     ELHASLDGLATHWPAAMLALSTHDTKRSADVRARLALLSQDPSWWTDTAAGLRRAAEA
                     HRRDGLPSDADVYLFFQTVIGAWPIDADRAAAYLAKAANEAKLRTTWTDPDPAYDAAL
                     EAFVRGALGDAAFVEAVESAVAHVRDAGWRASLAQLALQLTAPGIPDVYQGGELWDLS
                     LVDPDNRRPVDFALRRELLDEVESCSAPDAWSRRAEGLPKLWLLHRLLRLRRERPDGF
                     ADDAGYERVDVDDDAAIAFRRGEVLTVVPRFVLRAPTGWLELPEGRWRGLDARDHAGR
                     VAVAELTRDFPVAVLERAS"
     gene            complement(4556..6691)
                     /gene="glgX"
                     /locus_tag="VFV59_07020"
     CDS             complement(4556..6691)
                     /gene="glgX"
                     /locus_tag="VFV59_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEU5325616.1"
                     /translation="MALRVWRGNPYPLGATWDGAGVNFAIFSEHAESVELCLFDADGG
                     NEQRVRMLEQTDLVWHCYLPDVRPGQRYGYRVHGPYDPGAGHRFNPNKLLVDPYAKRI
                     DGAVTWDDALFGYRIGEDDLVPDERDSSPFVPKSVVVNEAFVWGGDRPLKRPLDETII
                     YEVHVKGFTQQHPDVAEELRGTYAGMGSAASIDYLTTLGITAVELLPVHQHVNDRHLV
                     ERGLSNYWGYNTIGFFAPEVRYSATGEPVSEFKTMVKRLHDAGIEVILDVVYNHTGEG
                     NHLGPTVSFRGVDNASYYRLSPEDLRYYVDYTGTGNTLDATSPRVLQLIMDSLRYWVT
                     EMHVDGFRFDLASALAREMYDVDKLGSFFDIIHQDPIISQVKLIAEPWDVGPGGYQVG
                     NFPVGWAEWNGKYRDTVRRFWKGIGSQTAELAYRLSGSSDLYAGSGRQPHASINFVTA
                     HDGFTLNDLVSYESKHNEANGENNQDGDNNNESMNFGVEGPTDEEGIIEIRERQKRNF
                     LATLLLSQGVPMLLGGDEIGRTQGGNNNGYAQDNEVSWYDWDVDRRDRQLLAFTRSLI
                     RLFRQHPVLRRRRFFQGRQIRGSRVKDLTWFAPDGSEMNDEQWQAPGVKTLGVQFAGD
                     AIDERGPRGERIVDDTLLVILNADERPVAFQLPNHDVAKRWEVVFDTVHPTFTAAHGE
                     HDGGAVYRVAERSVVCLRRQPRVRRRATG"
     gene            complement(6791..>7057)
                     /locus_tag="VFV59_07025"
     CDS             complement(6791..>7057)
                     /locus_tag="VFV59_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325617.1"
                     /translation="AGLLRSLDYAARTAADGRHASGFDADAWLAEARAAFLEAYGEIG
                     PEDAALLDAFELEKACYEVRYEANNRPDWLWLPLAAVERLAHGG"
BASE COUNT         1002 a         2455 c         2578 g         1022 t
ORIGIN      
        1 gcctcgccgt cgatccccat cgcgcgcatg acggccgcgc gggcctccgg gctgaccgtc
       61 cgggtccgct cgaacgcatc ccggtacgtc ggcaggacca ggtcgtcgct caccgcggca
      121 tcatcgccgc tcgacgagga cgacgacgcc atcgggcggc acggtcgcgg cggatccgtc
      181 gcccgcgtcg agagcgagct cgggcggcca cgccagccgg atgtcgccgg aggccgcgac
      241 cccggctggg ccggttccca ggttggcggc caccgtgacg cgaccgcgcc gaaccgtgag
      301 cagggagtca gaccgaccgt ccacccccac cgccgatcgg tcgccgtcgc gcagctccgg
      361 cagctcgcgg cgcagcgcga tcagggagcg gtaccactcg agcatcgcgg catgctcggg
      421 ctcgcggcgc tcgtcccacc ggagcttcga gtcgtggaac gtctccgggt cctgcgggtc
      481 gggcacgtcg gcggggtccc acccgaaatg cacgaactcg ttgcggcgcc cctcgcgcac
      541 ggcctgggca agctccggcg aggcgtggcc ggtgaagtac cggaacggcg tggaggcgcc
      601 ccactcctcc ccggcgaaga gcagcggcac gtacggcgac gtcagcagga gcgccgcacc
      661 gatgcgcgcg cgcccgggtg ataccacctg cgccaagcga tccccgcgcg cgcgattgcc
      721 gacctggtcg tggttctgga ggcaggcgat gaaccggtct cccggcacgt cgtcggcagc
      781 acgaccgtgg cgccggccgc ggtgggccga gtagcggtcg gcgtacaggt agggatcgtg
      841 gagggcgcgc ggcagcgcgg ccgcatcggt gaagtcaacg tagtagccgt tcgtctcgcc
      901 gctgagcgcc gcgtgcaggg cgtggtggac gtcgtcggcc caggccgcgt ccatgccgta
      961 gccgccgcgc gccggccgcc ggacgaggcg cgggtcgttg aggtccgact cggcggtgac
     1021 gaccaggcga cgccggagct cctcacccag ccgatgcacc gcggtcgcga tctcctcccc
     1081 gatctggacg gcgctcatgt cgaagatcgc ctgcatggcg tcgaggcgga gaccgtcggc
     1141 gtggtagtca cgcagccaga ggagcgcgtt gtcgacgacg aagcggcgca cctcgtggct
     1201 gcctcgctcg tcgaagttga cggcgtcgcc ccacggcgtg gcgtacttgt ggctgaagta
     1261 cggcccgaac cgcgacaggt agttgccctc ggggccgagg tggttgtaga ccacgtcgat
     1321 caggaccgcc aggccgtggg cgtgggccgc atcgatcaac cgcttcagcc cgtccggccc
     1381 gccgtaggcg tggtgcggcg cgaagaggtc gacgccgtcg tagccccagc cgcggtcacc
     1441 gctgaactcg acgaccggca tcagctccag gtgggtgacg cccaggtccg ccaggtccgg
     1501 cagccgctcg atcgcggcgt cgaaggtgcc atcgggactg aaggtgccga cgtgcgcctc
     1561 gtagatgacc gcatcggcca ggggtggcgc ctgccagccg ctgtccgtcc acgcaaaggc
     1621 gtcgtggtcg acggtgcgcg acgggccgtg caccccggac ggctgccacg gcgaccgggg
     1681 atcgggcacc ggctcgccgc cgtcgaggga gaaggcgtag tcggttccgg gcggcagctc
     1741 gacgtcagcg tgccaccagc cgtcctcgcg gcggcgcatg gcatggcgtt cggcgccgag
     1801 caccagctcg acccgctcgg ggcgcggtgc ccagacggcg ggacgcgtgc cggtcacgag
     1861 gcgcgctcca ggacggcgac cgggaagtcg cgcgtgagct ccgccacggc gacgcgcccg
     1921 gcatggtccc gagcgtcgag gccccgccag cgaccctccg gcagttcgag ccagccggtc
     1981 ggcgcgcgca gcacgaagcg aggcacgacc gtcagcacct caccccggcg gaaggcgatg
     2041 gcggcatcat cgtcgacgtc gacgcgttcg tagccggcgt cgtccgcgaa gccgtccggg
     2101 cgctcgcggc gcagccgcag cagccgatgc agcagccaca gcttcggcag cccttcggca
     2161 cggcgggacc aggcgtccgg cgccgagcac gactccacct cgtcgagcag ttcccgacgc
     2221 agcgcgaagt cgaccgggcg gcggttgtcc ggatcgacca gcgagaggtc ccacagctcg
     2281 ccaccctggt acacgtccgg gatgccggga gcggtcagct gcagggccag ctgcgcgagc
     2341 gatgccctcc agcccgcgtc gcgcacgtgg gccaccgcgg actccaccgc ctcgacgaag
     2401 gcggcatcgc ccagggcgcc gcgcacgaac gcctcgagcg ccgcgtcgta cgcggggtcc
     2461 gggtcggtcc aggtggtgcg cagcttcgcc tcgttcgcgg ccttggcgag gtaggccgcg
     2521 gcccggtcgg cgtcgatggg ccacgcgccg atgaccgtct ggaagaagag gtagacatcc
     2581 gcatccgacg gaaggccatc gcgtcgatgc gcctcggccg cccggcgaag gccggcggcc
     2641 gtatcggtcc accaggacgg gtcctgggag agcagcgcga gccgggcgcg gacgtcggcg
     2701 ctgcgcttgg tgtcgtgcgt cgagagcgcc agcatggcgg ccggccagtg cgtggccagc
     2761 ccgtcgagcg acgcgtgcag ctcgtcgggc ggcgtcccga agtggccggg gtcgccaccc
     2821 acctcgttga gcgcgaccag ccgatggtgg cggtagaagg cggtgtcctc cacgcccttg
     2881 gccatggcgg cgggcgtcag ctgctggaag cgcatggcca gctcagcggc cagcggcccc
     2941 ggctcctcga gccgcagaat ccggccgagg aacgcgaaga ggtcggggtc gaggtccggg
     3001 cgccgtgtga ccgcggtctc gaccgagccc gcgatcaggt ctcggtcgac ggacgagacg
     3061 tggccgtcgg cggcgcgaac gtaggtgcgg tagaccggga gcgcggcggc gacctcgcgc
     3121 agcgcctcgt gcacctcgtg ccgggtgaag tcgcgatagc ggcggttcgc ctcgcagatg
     3181 gcgagcagca ggtccgtcag ccggttgacg tcgctgccga ggaccgcggc gaggacctcc
     3241 ttgcgcgatc cctcgacgat gtcgtcccag ttcggtccag cgtcggcgac ctcggcccag
     3301 gcggcgctca acggctcctc gccggcggga tcgaccagga tgcccgtggc caggttggca
     3361 aagcggtagc cggtcgtgcc gtcgatgggc cagtccgccg gcagcgactc gccgggctcg
     3421 aggatcttct ccgccacgat ccacgcgtcg ggcgccgcct cgcgcagccg gccgaagtac
     3481 cccgcggggt cgcgcaggcc gtcgggatgg tcgatgcgca gtccctgcac ccgcccgtcg
     3541 cgaacccaac ccaggatccg ctcgtgggtg gcggcgaaga cgcgctcgtc ctcctggcgc
     3601 agtccgatga gctcgtcgat gtcgaagaag cggcggtacc ccaggtcccg tgacgcggcg
     3661 cgccaccgcg ccaggcggta gttctgacct tccaggaacg cgtccagccc gtcgacgtcg
     3721 gcattgagcc gctcgacggc ggcgtcgagg acgcccgcca cctccgggtc cgatgccagc
     3781 tcgccgagac gcgcctcgag gacgtcggca tcgcgctgcc ggcgagcgac ctgctcggga
     3841 tcggggtcgg tcgacgaggg gaggtccgac agcgccgagc cgatgaaggc cagctcgtcc
     3901 gagcccgcct cctccgcggc cttgctcacg atggcaccga cgctctgggg atcggtgggg
     3961 agcatccgct ccgatgcctc gaccagcagc gtgccctccc ggcggacgac gcggatctcg
     4021 cgggcctcga gcacgcgccc gtagtggtcg gccaggatcg gcagcaggac cacgttgcga
     4081 agccggctct ctggcgggtc ccagtcgacg tcgaagaagt gcgcgtacgc gctggccggc
     4141 ccgtggcgca ggacgtccca ccaccaccgg ttctcttcgg cgatggccat gtggttcggc
     4201 acgatgtcga gcaccaggcc catgccgtgc gcgcgcagcg catcggtcag gcggtcgaag
     4261 gcggcgtcac cgccgagctc ctcgttgacc cgggaatggt ccaccacgtc gtacccgtgg
     4321 gtggatccgc caaccgcctg gaggatcggc gacaggtaga ggtggctgat cccgaggctg
     4381 gccaggtacg gcacgacgtc gcgggcgtcg tcgagagtga agccggggtg gagctggagt
     4441 cggtaggtgg cggtggggac gtcgcgtggg cggtggcgca tcgggctccg tgggaaggtt
     4501 gggcgatcgg tggcggcatg catgcacgtc ccgcgccacg ctcagggacg cagggtcagc
     4561 cagtggcgcg ccgtcgaacc cggggctgcc ggcggaggca gacgacagat cgctccgcca
     4621 cgcggtagac cgccccgccg tcgtgctcgc cgtgcgccgc ggtgaaggtc gggtggaccg
     4681 tgtcgaagac gacctcccag cgcttggcca cgtcgtggtt cggcagctgg aaggccacgg
     4741 gccgctcgtc ggcgttgagg atgacgagca gcgtgtcgtc cacgatccgc tcgccgcgcg
     4801 gcccgcgctc gtcgatggcg tcgcccgcga actgcacacc gagcgtcttc acgccgggcg
     4861 cctgccactg ctcgtcgttc atttcggagc cgtcaggcgc gaaccaggtg aggtccttga
     4921 cccgcgagcc acggatctgg cgcccctgga agaagcgacg acggcgcagg accgggtgct
     4981 gccggaagag ccggatcagc gagcgcgtga acgccagcag ctgtcgatcg cggcgatcga
     5041 cgtcccagtc gtaccagctg acctcgttgt cctgcgcgta gccattgttg ttgccaccct
     5101 gggtgcggcc gatctcgtcg ccgcccagga gcatcgggac gccctggctc agcaggaggg
     5161 tggcgaggaa gttccgcttc tgacgctcgc ggatctcgat gattccttcc tcgtcggtcg
     5221 gaccctcgac cccgaagttc atgctctcgt tgttgttgtc cccgtcctgg ttgttctcgc
     5281 cgttcgcctc gttgtgcttc gactcgtagc tgaccaggtc gttgagggtg aagccgtcgt
     5341 gggcggtgac gaagttgatg ctcgcgtgcg gctggcgccc cgagccggcg tacagatccg
     5401 aggagccact gagccgatac gccagctccg cggtctggct gccgatcccc ttccagaagc
     5461 ggcgcaccgt gtcgcggtac ttgccgttcc actcggccca gccgaccggg aagttgccga
     5521 cctggtagcc gccgggcccg acgtcccagg gctcggcgat gagcttgacc tggctgatga
     5581 tcgggtcctg gtggatgatg tcgaagaagc tgcccagctt gtcgacgtcg tacatctcgc
     5641 gcgccagcgc cgatgcgagg tcgaaacgga acccgtcgac gtgcatctcg gtcacccagt
     5701 agcgcagcga gtccatgatc agctgcagca cgcgcggtga cgtcgcatcg agggtgttgc
     5761 cggtcccggt gtagtcgacg tagtagcgca ggtcctcggg gctcagccgg tagtacgacg
     5821 cgttgtcgac gccgcggaag ctgacggtcg gtccgaggtg gttgccctcc ccggtgtggt
     5881 tgtagaccac gtcgaggatg acctcgatgc cggcgtcgtg caggcgcttg accatggtct
     5941 tgaactcgct gaccggctct ccggtcgccg agtagcgcac ctcgggcgcg aagaagccga
     6001 tggtgttgta gccccagtag ttcgagagcc ctcgctcgac caagtgccgg tcgttgacgt
     6061 gctggtgcac cggcagcagc tcgaccgcgg tgatgcccag cgtggtgagg taatcgatgg
     6121 acgcggcgct tcccatcccg gcgtacgtcc cgcgcagctc ctcggccaca tccgggtgct
     6181 gctgggtgaa gcccttgacg tggacctcgt agatgatcgt ctcgtccagc ggccgcttca
     6241 gtgggcggtc accgccccac acgaatgcct cgttgacgac gacgctcttg ggcacgaacg
     6301 gcgagctgtc gcgctcgtcg ggcaccaggt cgtcctcgcc gatgcggtag ccgaacagcg
     6361 cgtcgtccca cgtcacggcc ccatcgatgc gcttggcgta cgggtcgacg agcagcttgt
     6421 tcgggttgaa ccgatgcccc gcgcccgggt cgtacggccc gtgcacgcgg tacccgtagc
     6481 gctggcccgg gcgcacgtcg ggcaggtagc agtgccagac gagatcggtc tgctcgagca
     6541 tgcgcacccg ctgctcgttg cccccgtcgg cgtcgaagag gcacagctcc acggactcgg
     6601 cgtgctcgct gaagatcgcg aagttcacgc ccgcgccatc ccaggtggcg cccagcgggt
     6661 aggggttgcc gcgccagact ctcagcgcca tgcgttcctc atcgggggga ctcgatcata
     6721 ggggtcgcgg cctcgcgcat gggcaggaac tcgctggccg ggcgcaggaa ccgcagtcgc
     6781 gagtccgcca tcagccgccg tgcgcgaggc gctcgactgc cgccaggggc agccagagcc
     6841 agtccggccg gttgttcgcc tcgtagcgaa cttcatagca ggccttctcg agctcgaaag
     6901 cgtcgaggag ggcggcatcc tccggaccga tctcgccgta cgcctccagg aacgccgcac
     6961 gcgcctccgc gagccaggca tccgcgtcga agcccgacgc gtggcggccg tcggcggcgg
     7021 tgcgtgccgc gtagtcgagg ctgcgcagga ggccggc
//
LOCUS       DASTYP010000156         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_522138, whole genome shotgun sequence.
ACCESSION   DASTYP010000156 DASTYP010000000
VERSION     DASTYP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522138"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /gene="leuC"
                     /locus_tag="VFV59_07030"
     CDS             complement(<1..1280)
                     /gene="leuC"
                     /locus_tag="VFV59_07030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU5325618.1"
                     /translation="MSTAPRPSTPRTIVEKIWDQHVVVAGTDGAPDVLGIDLHLVHEV
                     TSPQAFDGLRERGLRVRRPGQTVATVDHSVPTTDRSLPIVDEIAARQVQRLEENCLEF
                     GIPLHGIGSPHQGIVHVIGPELGLTQPGMTIVCGDSHTATHGAFGALAFGIGTTEVEH
                     VLATQTLLQRRPRTFEVRVDGQLHPGVTAKDIILSLLSRIGVGGGTGHVFEYRGSAIR
                     ALGMEARMTICNMSIEGGARAGLVAPDETTFEYVAGRPHAPTGAAWDAAVSDWRTLPT
                     DEGATFDRSIAFTADELTPMVTYGTNPGMGFPITERIPDPERLTDASQRDALRSALSY
                     MALNPGEPILGRPIDVVFVGSCTNSRISDLREAARMLRGRRVADGVRMMVVPGSTEVK
                     RQAEAEGLASVFREAGAEWREAGCSMCIAMNGDQL"
     gene            1344..2132
                     /locus_tag="VFV59_07035"
     CDS             1344..2132
                     /locus_tag="VFV59_07035"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5325619.1"
                     /translation="MATPEPQRISDPDWRDLLLGTHPDLARMRRIFRHLPADPRCKMC
                     LSPQGGIGGPVMSLLGYGRYPANPQLCNSCFRQSQRHPGGAEIEITALFADIRGSTGL
                     AESMSAAEYSAAVDRYVRVATHAIREPGGLVDKLLGDGVMALFIPGFVGPDHAAKAVE
                     AARQILRSVELPVGVGVHTGPAWVGFVGGIDDVQDFTALGDAVNVASRLGSEAGAGEL
                     LLSAATASAAELPTDGLDARRLELRGRSEPVDAWAERLEGSAAA"
     gene            complement(2148..3335)
                     /locus_tag="VFV59_07040"
     CDS             complement(2148..3335)
                     /locus_tag="VFV59_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325620.1"
                     /translation="MHEQQDPRRWLVLGIGLLGLIAGCAAQYGLAYLIPALRAEGLAL
                     EEATLLVSAPIVGILLTLIAWGAAADRWGERLVLGVGLAGAGLCALGAAAVDGLLARW
                     LLLFGVGATSSAIHAASGRLILGWFAASQRGLAMGIRQMGQPLGVGLAAVVLPPLATT
                     DVAPPLVALGVGCLVSAALVAALVRDPARPAVAAGGEGPRSPYREAYLWRIHLASALL
                     VVPQWTVAVFAFDFLVTARGWSIGDAGTLLAVTALLGAATRLAAGTWSDRARSRLGPM
                     RIVALVIGGAMVVLAATAIGDVAGTVVALAIAAVVTVSPNGLAFTAVAERAGSRWAGR
                     ALGIQNTFQNLVAGAAAPPLAVLIGLVGGGTTGYGVAFAVVAGLPFLAALAVPAREER
                     LSP"
     gene            3404..>3756
                     /locus_tag="VFV59_07045"
     CDS             3404..>3756
                     /locus_tag="VFV59_07045"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5325621.1"
                     /translation="MTDAAAPYDPREHSRILLDGPDRAAARAMLKAIGFTDEDLSKPI
                     IGVATTWIETMPCNYNQRELAERVKAGIRAAGGTPMEFNTIAVSDGVSMGTEGMKSSL
                     VSREVIADSIELVARG"
BASE COUNT          547 a         1388 c         1349 g          472 t
ORIGIN      
        1 agctggtcgc cgttcatggc gatgcacatg ctgcaacccg cctcgcgcca ctcggcgccc
       61 gcctcgcgga agaccgatgc caggccctcc gcctcggcct ggcgcttcac ctcggtcgag
      121 cccgggacga ccatcatgcg caccccgtcg gcgacccgtc gtccgcgcag catgcgcgcg
      181 gcctcgcgca ggtcgctgat tcgcgagttc gtgcacgacc cgacgaagac gacgtcgatc
      241 ggccgcccaa ggatcggctc gccggggttg agggccatgt acgatagcgc cgagcgcagg
      301 gcgtcgcgct gcgacgcatc ggtcaggcgc tcggggtcgg ggatgcgctc ggtgatcggg
      361 aagcccatgc ccgggttcgt gccgtaggtc accatcggcg tcagctcatc ggcggtgaag
      421 gcgatcgagc ggtcgaaggt ggccccctca tcggtaggca gcgtgcgcca gtcgctgacc
      481 gccgcatccc aggcggcgcc cgtcggcgcg tgcggacggc cggcgacgta ctcgaaggtc
      541 gtctcgtcgg gcgcgaccag gccggcccgt gcgccaccct cgatgctcat gttgcagatg
      601 gtcatccggg cctccatgcc cagcgctcgg atggcggagc cgcggtactc gaagacgtgc
      661 ccggtgccgc cacccacgcc gatccggctg agcaggctca ggatgatgtc cttcgccgtc
      721 acgccggggt gcagctggcc gtcgacgcgg acctcgaacg tcctcgggcg tcgctggagc
      781 agcgtctgcg tcgcgagcac gtgctcgacc tcggtcgtcc cgatgccgaa cgccagcgcc
      841 ccgaacgcgc cgtgcgtcgc cgtgtgcgag tcgccgcaca cgatcgtcat gcccggttgc
      901 gtcaggccca gctccggacc gatgacgtgg acgatcccct ggtgcggcga gccgatgccg
      961 tggagcggga tgccgaactc gaggcagttc tcctcgaggc gctgcacctg gcgggccgcg
     1021 atctcgtcga cgatcgggag gctgcggtcg gtggtcggga ccgagtggtc gaccgtcgcc
     1081 acggtctgtc cgggccgtcg gacgcgaagg ccccgctcac ggaggccgtc gaaggcctgg
     1141 ggactcgtga cctcgtggac gaggtggagg tcgatgccga ggacgtccgg cgcgccgtcg
     1201 gtgccggcga cgacgacgtg ctgatcccag atcttctcga cgatggtgcg gggcgtgctg
     1261 gggcgcggtg cggtgctcat gggtgcctgc gaccctagcc gatgcgtcaa gtggtcgctg
     1321 gacgaacggg ataccctgtg ccgatggcca cccccgagcc gcagcggatc tccgatccgg
     1381 actggcgaga cctccttctc ggaacccacc ccgacctggc ccgcatgcgc cggatcttcc
     1441 gccacctgcc cgcggatccg cgctgcaaga tgtgcctctc gccgcagggc ggcatcggcg
     1501 ggccggtgat gagcctgctc ggctacggtc ggtaccccgc caacccgcag ctctgcaaca
     1561 gctgcttccg gcagtcgcag cggcatcccg gcggggcgga gatcgagatc accgcgctct
     1621 tcgccgacat ccgcggctcc accggcctgg ccgagtccat gtccgcggcc gagtactcgg
     1681 cggccgtgga ccgatacgtt cgcgtcgcga cccacgcgat ccgtgagccg ggcggcctcg
     1741 tcgacaagct gctcggcgac ggcgtgatgg cgctgttcat ccccggcttc gtcggcccgg
     1801 accacgcggc caaggccgtc gaggccgcgc ggcagatcct gcgctccgtg gagcttccgg
     1861 tgggtgtcgg cgtccacacc ggaccggcat gggtgggctt cgtcggcggc atcgacgacg
     1921 tgcaggactt caccgcgctc ggcgacgccg tcaacgttgc cagccgcctc ggctcggagg
     1981 cgggggccgg ggagctgctg ctgtcggccg cgaccgccag cgccgccgag ctgccgaccg
     2041 acggcctgga cgcccgacgg ctggagctgc gcggccgttc ggagccggtg gacgcctggg
     2101 cggagcgtct cgagggttcg gcggccgcct gagccggcgg ccaacgctca gggcgacagt
     2161 cgctcctccc gcgccggcac cgcaagggcg gcgaggaacg gcagcccggc cacgaccgcg
     2221 aacgccacgc cgtaccccgt cgtcccgccg ccgaccagcc cgatcagcac ggccagcggc
     2281 ggtgcggcag ccccggccac caggttctgg aaggtgttct ggatccccag cgctcggccc
     2341 gcccagcgcg agccggcccg ctcggccacg gccgtgaacg ccaggccgtt gggcgagacc
     2401 gtgacgacgg cggcgatggc cagcgccacg accgtcccgg caacgtcacc gatcgccgtc
     2461 gcggcgagca cgaccatggc cccgccgatg acgagggcca cgatccgcat cggtccgagg
     2521 cgtgaacggg cgcggtcgga ccaggtgccg gcggccagcc gggtggcagc gcccagcagc
     2581 gcggtgacgg ccagcagggt gcccgcgtcc ccgatcgacc agccgcgcgc cgtgaccagg
     2641 aagtcgaacg cgaagacggc caccgtccac tggggcacca ccagcagcgc cgaggcgaga
     2701 tggatgcgcc acaggtacgc ctcacggtac ggcgagcgcg ggccttcacc accggccgcg
     2761 acggcaggcc gggcgggatc ccggacgagg gccgccacga gggccgcgct gaccaggcag
     2821 ccgacaccga gcgcgaccag gggtggtgcc acatccgtcg tggcgagcgg tggaagcacc
     2881 accgcggcca gcccgacgcc gaggggctgg cccatctggc gaatgcccat cgccagcccg
     2941 cgctgcgaag cggcgaacca gcccaagatc agccggcccg acgccgcatg gatggccgag
     3001 ctggtggcgc ccacaccgaa cagcagcagc caccgcgcca gcaggccgtc caccgcggcc
     3061 gcgcccagcg cgcacaggcc cgcaccggca agcccgacac cgagcacgag ccgctcgccc
     3121 caccggtccg ccgccgcgcc ccacgcgatc agcgtcagga ggatgcccac gatcggggcg
     3181 ctgaccagca acgtggcctc ctccagcgcg aggccctccg cgcggagcgc cgggatcagg
     3241 taggcgaggc cgtactgggc agcacagccg gcgatgagcc ccagcagccc gatgcccagg
     3301 acgagccatc ggcgcgggtc ctgctgctcg tgcatcatgc cattgtgcga gccgtcgccg
     3361 agcgcggttc gcgggactgg acacacctcc tagactcccg cgcatgaccg atgccgccgc
     3421 cccctacgac ccgcgcgagc actcgcgcat cctgctcgac ggccccgacc gggccgcggc
     3481 gcgcgccatg ctcaaggcca tcggcttcac cgatgaggac ctgtcgaagc cgatcatcgg
     3541 cgtggcgacg acctggatcg agacgatgcc ctgcaactac aaccagcgcg agctggcgga
     3601 acgggtcaag gccggcatcc gcgcggcggg cgggacgccg atggagttca acaccatcgc
     3661 cgtctccgac ggggtgtcga tgggcaccga ggggatgaag tcgtcgctgg tgtcgcgaga
     3721 ggtcatcgcc gactcgatcg agctggtggc gcgcgg
//
LOCUS       DASTYP010000157        34337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1214307, whole genome shotgun sequence.
ACCESSION   DASTYP010000157 DASTYP010000000
VERSION     DASTYP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 34337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34337
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214307"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VFV59_07050"
     CDS             complement(<1..117)
                     /locus_tag="VFV59_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylene tetrahydromethanopterin
                     reductase"
                     /protein_id="HEU5325622.1"
                     /translation="MQFGIFSVSDITTDPTTGRTPTEHERLEAVLAMASKAEE"
     gene            complement(194..529)
                     /locus_tag="VFV59_07055"
     CDS             complement(194..529)
                     /locus_tag="VFV59_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325623.1"
                     /translation="MGWQLLGAAIGVALMAAPGILTLPAAVADAFHVLGPLAAACGAI
                     AASPVLRVLRRIQLPVGLGVAVAPLVLGSDVVGIGVGLLAGLALLATAFAPGAPMDRF
                     GGGWRALTG"
     gene            complement(529..1074)
                     /locus_tag="VFV59_07060"
     CDS             complement(529..1074)
                     /locus_tag="VFV59_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEU5325624.1"
                     /translation="MAEAGAPPGWSYNPSRWGERIPILVLALIGLGIASYLAAFQFGL
                     TRDVFDPFFGDGSRTVLTSGVSRVLPIPDAALGAVGYITDVASGVVGGRERWRTMPWI
                     VILFGLAVGPLGATSIVLVILQPVMFDAWCTLCLASAAISIAMIGPAFDEVLASAQHL
                     RRVHQRGGSVWRAFWAGEGAG"
     gene            complement(1067..2083)
                     /locus_tag="VFV59_07065"
     CDS             complement(1067..2083)
                     /locus_tag="VFV59_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5325625.1"
                     /translation="MPQPQTVVITGASAGVGRALARIFAARGDRVALIARGTDGLEGA
                     RRDVEAAGGTALVLPLDVADADAVEAAAETLERELGPIDVWINNAMASVFSPIDEMTP
                     DDFRRVTDVTYLGAVWGTLAALRRMKPRDRGTIVLVGSALAYRGIPLQAAYCAAKHAM
                     QGFHDSLRTELEHDRSNVKVTMVQLPAVNTPQFRWVKSRLPHKAQPVPPIFQPEVIAE
                     GIAWAADNPDATRELIIGWPALKAIMGNKVVPGYADRVLARTGYASQQIDEPEDPNRP
                     NNLWQPVDGPGGGDHGAHGVFDDRARDSSWQLWANTHRPTLVLSALGTAAVAGLAALG
                     LRRG"
     gene            complement(2098..2568)
                     /locus_tag="VFV59_07070"
     CDS             complement(2098..2568)
                     /locus_tag="VFV59_07070"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEU5325626.1"
                     /translation="MTRHSRPLSHAMGLGALAGLIATLAMSAVMLVADRLGLMAEHPP
                     ERIVERGSKATGTPTSEGQQNALASAAHLAFGAAGGAGFGLLAQLVRPAIPGALALPW
                     ALAIWAGSYFGWIPALGILPPPTRDSPGRASTMLVAHLVYGLSLGVMWRAVTRR"
     gene            complement(2609..3556)
                     /locus_tag="VFV59_07075"
     CDS             complement(2609..3556)
                     /locus_tag="VFV59_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5325627.1"
                     /translation="MATNLGVLLWSQSSGWDEFEASARRVDELGYDHLWTWDHLYAIF
                     GDPYQPIFEGYTTLAAWAKVTRRARLGLLVGANTFRNPGLVAKTLTTIDHLSGGRAIA
                     GIGGAWFELEHTEHGIDFGSGFGERLDRLDEAVAALRALFAGEEVTSPPGSTYAFERL
                     RLLPPPVQERLPIMIGGSGEKKTLRTVARYADMWNAMGEPDFLRRKVEVLRRHCDEVG
                     RDPAEIEFTAGCKPLIRDTEAEARRVWEAQMAHNRTPMTDVEDDDTFWVGTPEQVAEE
                     MHRRKELGFHTFIAEMAAPYDEETLERWIGEVRPMVDGG"
     gene            complement(3579..4667)
                     /locus_tag="VFV59_07080"
     CDS             complement(3579..4667)
                     /locus_tag="VFV59_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5325628.1"
                     /translation="MTTGDAPRAASAMDREIHDIPATVAALLDRVGPEVAAAAEDFRA
                     ERPAWITIVARGSSDHAAVYARYLIETVLGIPTGLAAPSVLTAYGARLRWADGAVIAI
                     SQSGQSTDVVEVARAARDAGAVTLAITNDPASPVAGAVHRVVLCRAGREDAVPATKTY
                     VTQLVAAASFVATLAEDGALLSQLRRVPEVASQTLIAAERWIADPEAGGAAVDAIASV
                     GRAIIVSRGFNLATALEIGLKLKETARVFAEAYSAADVLHGPMVLADDAVPMLVVRPE
                     GPVASSIDAASAAAGRRGTEPWVIGIGEGGADRPRPRDLSLATGLQESLTPIVGVLPG
                     YLLAEASARRLGLDPDAPAGLQKVTKTR"
     gene            complement(4664..5935)
                     /locus_tag="VFV59_07085"
     CDS             complement(4664..5935)
                     /locus_tag="VFV59_07085"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU5325629.1"
                     /translation="MGLKVAVIGAGSTYTPELVEGLASRSERLPLDELVLEDVDAERL
                     GVLGGMAERMLRRLEWPGRLTTTGRIEEAIDGADFVLLQLRVGGQAARLVDETLPLRF
                     GTIGQETTGAGGFAKALRTVPVVLDIADEVKRRSRPGAWIIDFTNPVGIVTQALLDAG
                     HRAIGLCNVAIGFQRRFARLFDVGPDEIELDHVGLNHLSWIRGVRVRGVERIDEVIER
                     IGSDLERDIGIPVERMRAQRAIPSYYLRYYERFEDVLAEQRAKTSRAQEVMDIEAKLL
                     ELYRDPSVDTKPELLEHRGGAYYSEAAAQLLASLHDGSGDLQVVDVRNGGAVPGVPAD
                     AVVEVTSRIDRDGAHPRSVDPLSPEMLELVRSVKAFERLVIGAALSGDRGDALRALAA
                     NPLVGPSTDPALLLDAILEANRAMLPRFFPE"
     gene            6037..6885
                     /locus_tag="VFV59_07090"
     CDS             6037..6885
                     /locus_tag="VFV59_07090"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5325630.1"
                     /translation="MAQFRLEHGPIPLHHQVYLDVRRALAGGEWSAGDRLPTERELAT
                     RYGCSLITVRRALDELAREGRIERTRGRGTFVLASRIEHDFAGQLSFSEDMRRRGLRP
                     STRLVERHVEPATDLVAEALGIAPGSPVVYVERVRMADDEPLLLEQARLSAERFPTLL
                     QADLERESLYDLLLHRHGTRVVRSSETIEPVRVPRREADLLAVRPGSLALLVIGTAAT
                     VAGPPVEYTRSFIRTDRTRYRLDRVIQRPGWARSFETGVRSAAASPSAPGGKKGGAPI
                     AERVSR"
     gene            6923..8344
                     /locus_tag="VFV59_07095"
     CDS             6923..8344
                     /locus_tag="VFV59_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5325631.1"
                     /translation="MVKRRLVILLPLLALVVSACGGQTTTTSPGESASGSAASDGASG
                     DVVEVRWYCCLGTGEDAESQLPVENRIVEEFNASHPNIHLTFEVVTYDEARDTLATQL
                     ASGNPPDIVGPVGIGGAEAFHGEWLDLTPLIEESGFDLGRYEQAAVELYQSGGEGQVG
                     IPFAAYPSVMFYRKSLFEEAGLNEPPHEYGEPYVWPDGTEAEWNYDTVRELALRLTVD
                     ENGLDATQDGFDPTAIVQYGYEPQRDDIRGLGAYFGAGSIASEDGETAQIPEPWAAAW
                     KYWYDGMWNDGFILTGPDYDSDEWAGQVDYAFFTGRVAMSTNFLWSTYGVAEGGDDWD
                     VAAVPSYEGTTTSPLNADTFRIMKDSAHPEEAFEVLRYFLEDASADLLNIYAGMPAVP
                     EEQDAFFETYQEQFPNEVDWQVVKDSLQYADVPNWEGYMPAYNETLDLLLTYGDKWAT
                     TDGLDMDEEIATLQDEIQAIWDR"
     gene            8396..9295
                     /locus_tag="VFV59_07100"
     CDS             8396..9295
                     /locus_tag="VFV59_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5325632.1"
                     /translation="MTPLARREARWGYLFILPWVIGFAAFTALPMLATLGFTFTNLTL
                     TQDEPLTFVGIDNYRHLFSDPQMWRSLGVTLRFAVLWLPTVILAPFAVALLLNAKSLR
                     GSRFFRVLFFLPYVVPFVAGVFLWQSMLANDGWVNDVLRFLGLAQPPNWLFDRDWIYP
                     ALVVIGIWGIGAGIIINLAGLQGIPAELYDAAQIDGAGWWAQLRNVTIPFMTPIIFYS
                     LVLGIVEVMQYFLVPLVLKSGTGEPGGATLFYNLYLYKNFFTFQNMAYGATLAWFLFV
                     IILAATLALFWSARRWVFYAGER"
     gene            9297..10316
                     /locus_tag="VFV59_07105"
     CDS             9297..10316
                     /locus_tag="VFV59_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5325633.1"
                     /translation="MRSPKALAVTLFTVLLAGVFLSPFLRSAMIALSTREQLGQANAP
                     IWPANPATFEYQGREMVIYEVPFDDGTRDLALLRPGRTSSEFIDPADPETVIPWEGSW
                     RALERSWTVAPTWSNFSDVWVLIDYPRVLFNTIALAVIGTIGTVLSCTLVAYGFARFR
                     FPGRGMLFTLLIATIFLPAAVTLIPTYTVFVKLGWVGTWLPILVPAFFANAYDVFLLR
                     QYLMTIPRDLDEAASIDGAGPFRILWSVILPQAWPAVVTVGIFTFVYTWNDFFGPLIY
                     LSTRPDLQPISVALARFNGLYSSNPALIQAGTLMTLILPVLLFIVFQRFFIRGIALTG
                     SGVER"
     gene            10313..10975
                     /locus_tag="VFV59_07110"
     CDS             10313..10975
                     /locus_tag="VFV59_07110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5325634.1"
                     /translation="MSAGFRVALTFDAEHPDRPNDGLGAARIIETLAAAQTTGTFFVQ
                     GRWAEANPTMARRIVTDGHLVGSHSQYHARMPLFTPRGFATDVRAAEGAIRRVARVDP
                     RPWFRLPFGSGATSPEVIAALAALGYRHVGWHVDVKEWRRRETIASVRDRALELVRAH
                     GDGAIVLLHTWPDPVGLALPALIERLASDGARFVRVDELGLGDGLEPVAEPRPADATT
                     RR"
     gene            10972..12018
                     /locus_tag="VFV59_07115"
     CDS             10972..12018
                     /locus_tag="VFV59_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEU5325635.1"
                     /translation="MSAPVAILGVDGGNSKSEIVLAARDGTVLARRRGPTISHQSIAP
                     GHGLTPAQRASLGMERLAQLTRDAAREAGLVPDANPLADVAVLCLAGADLPSDVRLLG
                     SALAAQHLLERPVILNDSFAPLRAGTERHYGISLICGAGINAAAIAPDGRTAGFPGLG
                     TISGDWGGASGLGMAGLVAAVRARDGRGPRTLLEELVPRHFGVMRPATVTRRLYEGEI
                     DEERVRELAPVVFEAAIGGDRVARGIVDHLADELATMAIALARRLGMTRTAFDVVLAG
                     GIFRTRDRGLFDRIGERVATALPHATLRRLDAPPVLGAALLALDHLPGAEPGAEERLR
                     AGVVALDPPPDAAA"
     gene            12018..13202
                     /locus_tag="VFV59_07120"
     CDS             12018..13202
                     /locus_tag="VFV59_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325636.1"
                     /translation="MSRGHERWRPVAGYLAQYSAVGALYPFLPVYYATLGFGLQAIGL
                     LGAAFAVAALVGAPLWGATADHLGAARPVLSVAALAASVSAAGLALSQGSVPIVLSAV
                     ALSLMMAGIGPILDARAMEIVGAGRGSYGRMRVWGSLSFIGAVLLTGWLVEAFGISTM
                     FAVLVIALVATAVVGFGIRSRPAVAPLPGRAALAAVARTPMIMAFLGVVLLAWTSSTA
                     INAFFSIHLVDIGAPESLVGASWALGAVVEVPIMIAFPSLQRRIGLERLLIAGAALFA
                     LRGVAVVLTGDPLLTTLTMLIHGAAFALVLVGGVVYVSTHAPRGAAASAQGMLAATVF
                     GLAQVLGPGIGGVVARSGGLPTLFAIATAGSLAATVALAAVIGREQPGVPAGVRREGS
                     AS"
     gene            13199..14095
                     /locus_tag="VFV59_07125"
     CDS             13199..14095
                     /locus_tag="VFV59_07125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5325637.1"
                     /translation="MKFGLRIDDFGWPGGGRRMAEHLASIGRRAEDAGFDSIWVWDHF
                     VQLRRWEEPLLEGWMALAYLAGVTRRVRLGTHVTGVTHRAPAVLVKQATTLDVLSGGR
                     AYFGVGAGWNEREHDAFGIPFPPLRERFELLEDTLQLALRAWSGERTPFVGRHVRLTE
                     QIDVPQPMQRPHPPILIGGVGERRTLKLVAQYAQATDLWLSDPDQIRHKLDVLRRHCD
                     AVGRDPDHIERYARVDLTMPHGEEGEVAPAPILLDRIAATAAAGADAIVVPLPRMAEP
                     GVIEQFGSEVITPARGIARPPG"
     gene            complement(14104..16110)
                     /locus_tag="VFV59_07130"
     CDS             complement(14104..16110)
                     /locus_tag="VFV59_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5325638.1"
                     /translation="MTDVHPYLEVRERKRGRRAGDVYVRIERPFDHEFQRAQAGHLVA
                     TEETLRAGGRIGAAWRAARIFLFGRPISSEMEEHERLTKVKALAVFSSDNISSSAYAT
                     EEMMRIVVLAGIGALSMVVPLTLVIVAVLAIVATSYRQTIKAYPNGASSYIVAGDNLG
                     APAGLVAAAALLIDYTLTVAVSVSAGVAAITSIAPGLFDVRVLLAVGIVVLLTIGNLR
                     GIRESGSIFMAPTYVYLVAVFGMLGYGLWQMATGTLAPYQPPREWLEAEAAVQPLGIL
                     LVLRAFSSGAVALTGVEAISDGVPAFKPPEWRNARTTLTWAAALFGSLFLGISILTSS
                     IGIVPDPSEEQTVLSLVTRHIVGNGWYLVLVQVATALVLTLAANTSFADFPRLSSFLA
                     RDGFMPRQFGYRGDRLAFSTGIVALASLAVVLLVAFGASVTALIPLYTLGVFIAFTLS
                     QSGMVVRWWRRREPGWTHSMAINALGAATTGVIVVIVGVSKFTQGGWIVMVLIPLVVL
                     LLWGIRTHYQHMNSSLALDAPTAQLAVRRRPIVVVPVARLDQPALQAVAVAHALDPEA
                     LAVHITNDAAEAESLRRQWPELVGAGELVIVESPYRALLQPLLRYLDALQRQDPTRPV
                     LVVVAEVVPRHWWEGLLHNQTALRLKFRLFFRRNTIVTDVPYHR"
     gene            complement(16107..16319)
                     /locus_tag="VFV59_07135"
     CDS             complement(16107..16319)
                     /locus_tag="VFV59_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325639.1"
                     /translation="MLRTACQALAGTDAEVTAIDVVSHIPDGVPATALLASVRGIAAE
                     YGCRASVAIANGALEVRLSRDGAMRR"
     gene            complement(16475..17191)
                     /locus_tag="VFV59_07140"
     CDS             complement(16475..17191)
                     /locus_tag="VFV59_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5325640.1"
                     /translation="MTEAASTGARVLVLDDEEAIRRTVERLLTGHGFVVRAVSSGEEA
                     LGVAPRFQPDLMLVDLGLPGVDGFEVIAQTRDRTSTPIIVLSVRGAERDKVRALEMGA
                     DDYLTKPFGVDELLARVRVALRHAARGGRGTDAIFRSGGLAVDLERREVTVDALPVQL
                     TPTEYALLTALVDQAGRVLTDRALLRRVWGPEYGSESHYLHVYMARLRKKLEADPANP
                     RYLRTEPGVGYRLLEVDAEG"
     gene            complement(17188..18708)
                     /locus_tag="VFV59_07145"
     CDS             complement(17188..18708)
                     /locus_tag="VFV59_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5325641.1"
                     /translation="MLPAPVAVVLPYLLATAGVAMVTLGIALAGRPDLGNASMLYLIV
                     VLVAAVAAGRAVAIFSALLAFFSFNWFLTEPRHTLSVADPDDWILLLLFLLTAVVTGQ
                     LAAGQKRRAEEAEEHERQARVLTDIAIALTEPTLERTLRSVADRLRAELGVDAVQVEL
                     GGARAASSVDADALEALDNTDRTIDVLATDGGSEARWIRLAPPHRRLAAMAMAHRVGR
                     APIRGAGGEEAGHITLATSNRRGFDGAAARLLAGVAPQLWAAQERSRLRAEATEAEVL
                     RRSDEAKSALLDAVSHDLRTPLASIIASAGSLRQAAVQWSTQERAEFAAAIEQEARRL
                     DRIVGNLLDLSRIRAGSLHPDVAWYEPVALVRDVLARMAGVTAAHRLVLDLPDELPPV
                     PLDYSEIDQVLTNLVENAVRHSPPGTTVTVRAVVDGGELRVCVEDEGPGIPVDELRRI
                     FEPFHRVRGVTRRGTGLGLAVARGLVEAHRGRIWAESAAAGGSRFCFVIPTREAMP"
     gene            18845..19948
                     /locus_tag="VFV59_07150"
     CDS             18845..19948
                     /locus_tag="VFV59_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate flavodoxin/ferredoxin oxidoreductase"
                     /protein_id="HEU5325642.1"
                     /translation="MREYVDGNAAIVRGAVAAGCTFFAGYPITPASGILMAMMRELPA
                     RGGVAIQGEDEIASIGMCIGAAMAGSRAMTATSGPGMSLMGENLGLAIMGEVPLVVVD
                     VQRLGPATGGATTVGQGDVQFARWGTPGGFPTIALAPTDAADAYRLTIRAFELADRFR
                     CPVVLLTDKEVATTLTTVDAAAFAPVAVVERELATAGAPYRYAPAADVPALRRYGAGD
                     AFRFTGSTHDERAMITKDRPTVDALNRHLAAKVEKHVEELTLVELDWQPGARTLVVSY
                     GVTAGAARAAVEAARQRGRAVASLVIRSLWPVPEAAIRAALGGIERVIVPELNLGQYR
                     REIERLTGDREVIGVNRVDGDLIGLEEILEAVG"
     gene            19945..21273
                     /locus_tag="VFV59_07155"
     CDS             19945..21273
                     /locus_tag="VFV59_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5325643.1"
                     /translation="MTVVSGDLATDLTTLETFRNALPYPFCPGCGHGGVLDRLDEALR
                     RLGRDPARTVLVSDIGCSGLSDQYFVTNAFHGLHGRSITYASGIKLADPALEVIVLMG
                     DGSAGIGGAHLLAAARRNVGITVVVMNNLNFGMTGGQHSTTTPSGAVTSTTPGGNLEQ
                     PLDLCATVAVNGAAYAHRATQWDDDLADRLVEAIETPGFALLDVWELCTAYFVKGNRL
                     SRRGMEALMEERGFRRGTVVHRAVDEYAAAYRHAHQADAPAAPPAPIAIDHAATLDRP
                     YGVVVAGSAGGRVRSAARLIAEAAVRCGLWATQRDDYPVTVQAGHSVTELLLSPAPQP
                     LAALETPELMVVLSEDGRRRVSSTLRRLRADATVVTVAGVAAPETPARVVVADPGRLA
                     RGDATLAVLVTAITALGVLPAQAIRAAASAGAHASANLAAIEAATSLPLG"
     gene            21351..21584
                     /locus_tag="VFV59_07160"
     CDS             21351..21584
                     /locus_tag="VFV59_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325644.1"
                     /translation="MREEHPHGRPVEDRAFEAVEIAAGVAVGAGLGVAVAGPVGAAVG
                     GLVGAAVGAIGGEALERAEGRAAETTDATAVVD"
     gene            complement(21595..22086)
                     /locus_tag="VFV59_07165"
     CDS             complement(21595..22086)
                     /locus_tag="VFV59_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325645.1"
                     /translation="MRRRGTRPAETLGGADSDYEHLFLSAWGLHPDQWRPVGSMALIR
                     QRATFIWRIDGPHPVEDVSLRARRAYDRLGELGTTVSAESTADGIEVVLPLGRVAVAL
                     RTLAALSTLPDAVRVEDAPPAQLRELRRCIAGFRPQPELLIGTHERTDVLAALRAAAR
                     RLA"
     gene            22256..22711
                     /locus_tag="VFV59_07170"
     CDS             22256..22711
                     /locus_tag="VFV59_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325646.1"
                     /translation="MTVSEGAVTLLWVNADEAVAWRWQEGHEQLLGRMESEVPTHHRS
                     MGHVRRASGDRHGGSGPRSAQEGHRLEHLRAFLAEVRAALPPDDDLLLLGDGTVVDQL
                     GARIREEDTHHRRTRTVEVHHAERMTDRQLIARLRGFAADPPRRVLARR"
     gene            complement(22725..24044)
                     /locus_tag="VFV59_07175"
     CDS             complement(22725..24044)
                     /locus_tag="VFV59_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5325647.1"
                     /translation="MSGNRGPAIHAGDARLLSGVRWRLVLFSAGSTLVLLVVLGSALY
                     FSTQASLAASATTQLEARVADARRILLGQARPEPDEDDEQFGQFLFGGPASGTALLLF
                     DAAGRQVLPEATTISPDLPVGAGFAAALDGQRDLRTATISIGAGDEHDTPVRVLSEAV
                     DVGGETYVLQVLQDRSTEARTLSVVLAVLAIGGLAALLAAAGFGFLYAGRALVPIRES
                     LRRQREFAADASHELRNPLAVIRGSVERLRRHADRPVSSMADALDDVEAEVRQLTGLV
                     DDLLMLARSDTGAIELQRVPVDLADLAGEALGSVRPVADQVGVRLRLDAAPAPLVGDP
                     DRLRQVVVNLADNAVRHSPTGGTVTVTVRGGRGAHLLVEDEGAGIRDADLPHVFDRFW
                     RASGAPAGGTGLGLAIVAWIVERHGGRVRAANRQGSQGARFEVRLPA"
     gene            complement(24041..24721)
                     /locus_tag="VFV59_07180"
     CDS             complement(24041..24721)
                     /locus_tag="VFV59_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5325648.1"
                     /translation="MHLLVVEDDPRLGRLLQRLLEDDRHLVELTDSGETAAETIEANR
                     ELDAVILDLGLPDISGIEVAQRVRRAGVRTPMLILTARDAIEDRVAGLDAGADDYLVK
                     PFAYQELAARLRALARRGSVERATDEPVLRNGPIRLDEARRLVSVDGRPIELSPREFS
                     LLECLLRHPGQVMTRDQLLDAAWPYGEFLTHNTVDTYVHYLRSKLGADAAKRIGTVRG
                     VGYRMAPG"
     gene            complement(24847..25275)
                     /locus_tag="VFV59_07185"
     CDS             complement(24847..25275)
                     /locus_tag="VFV59_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5325649.1"
                     /translation="MSTTHALTPMRPVPPLVVADLMSLEPIVVYESESIANVDAVLRD
                     YGISGVPVINRHGTIVGVYSLTDALRLKDINPDALRRYSVGDVMTRPAITVDREVSLQ
                     DAARLMVDHRVHRVVAVDDAGRPVGVLSAMDFVELAAETA"
     gene            25736..26692
                     /locus_tag="VFV59_07190"
     CDS             25736..26692
                     /locus_tag="VFV59_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEU5325650.1"
                     /translation="MIGIGLLALAAVLLFAGAELFADNAAGAGRRLGITALAVALLLA
                     GAEPEEMITAVLAAISGRPDLAAGDAVGANVTMLTLVLGLTAVLRPVPFGNRVREYAA
                     GAAIAGVAAVVVLLDGGVSRLEGLALITVYSALVALVWWREREPPLIGEAAEVVEDAD
                     APAPQGTAWMPLALAVAGIGIMLGGGWVAVEGATRLVGDLGIRDSVIGLTLLALATTA
                     ELFALAWSAARRELSELAVAAVIGSAGYNATVSLGSAAVAQPLAVSGVLGAAIAGAAL
                     PILLIGLGWSGRVGRAGGATLLAGYAVYLFVVLTGSGGVTPS"
     gene            complement(26708..26944)
                     /locus_tag="VFV59_07195"
     CDS             complement(26708..26944)
                     /locus_tag="VFV59_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325651.1"
                     /translation="MSLSSSQLDHRRRAYRIDHEPTCDRCGHGASLHTLDERYPCIGC
                     GERAAMGASPRPVCIGFASAWFDNSTRRLAARTR"
     gene            27220..27636
                     /locus_tag="VFV59_07200"
     CDS             27220..27636
                     /locus_tag="VFV59_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325652.1"
                     /translation="MSKATVTRIFVGSVIAVVAGIILAIVALLVAYANGAFVMDGPDV
                     VGIRGTTFAWTMVGLAVVAGLVVMAGFVGGMVAWVGALLNTAQLTDKTWFIILLLLGI
                     WNLGVLAMIAYLIAGPDGTAAAPDSDAALRRETSHA"
     gene            27781..28068
                     /locus_tag="VFV59_07205"
     CDS             27781..28068
                     /locus_tag="VFV59_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325653.1"
                     /translation="METAFNIFVFLLFAALWVGFAYALIASQGSLNAAWDWITRQHVV
                     VQGVVWLLFLPVVAGLWIWHTDWALVVRLVLVVGIGFWNIYLFFPSYLLRR"
     gene            28400..28849
                     /locus_tag="VFV59_07210"
     CDS             28400..28849
                     /locus_tag="VFV59_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325654.1"
                     /translation="MTSGVDSSNVGFVNRDLLVTCAALACLLAACSSIREVWPTDPGD
                     VGCPNSVEAARAVTLGDNAFVATGYVIRFVPSPDEAFRGYDLDVTRVFTGPAFRDVGF
                     LRIEDEIAGMSQGDPVLVVAERTDKARVFVPGPCPPLVRVPAEDVEP"
     gene            28846..29268
                     /locus_tag="VFV59_07215"
     CDS             28846..29268
                     /locus_tag="VFV59_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325655.1"
                     /translation="MIGRVAVAGVLLLGGCGSVAPRPSVGEAIFFPTHEHTPQTGLPA
                     GAIAGTLALEDGCLWVVTDDNTPLLALWPSDFRLETEQGRLIVIGRGGSVPLGGRIHA
                     SGGEYATDQVAFVEQQISAEIPDACRGRGFWLVGELMD"
     gene            complement(29321..29818)
                     /locus_tag="VFV59_07220"
     CDS             complement(29321..29818)
                     /locus_tag="VFV59_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325656.1"
                     /translation="MAGSWFTSKLGLVVVAILLLAGCAPPDNPGRRSEAVDLAERYLR
                     ALSGAEAGRGWSLLHQSARDEWGSQEEYVAAAADADWSQFEFTVLEAVYCDDGIFCSV
                     ALDVPNGRESLAEPFQSPDGQHSSGILFRSVEGLPGNAELMVVLPHWMRGPGGVRAGR
                     RASAP"
     gene            complement(29829..30239)
                     /locus_tag="VFV59_07225"
     CDS             complement(29829..30239)
                     /locus_tag="VFV59_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325657.1"
                     /translation="MRRLAFLPLALALVGCGLTQDRSAAMCEVAGGLEHAYGVAENAL
                     GADRAGDTERARQLASDASGLADEAKARLDAVDGDATVASDPYQALVMGYLHVGQAAK
                     ALLPEWANTHGMTAEELDLARTQLGAAEAALDGC"
     gene            complement(30243..30659)
                     /locus_tag="VFV59_07230"
     CDS             complement(30243..30659)
                     /locus_tag="VFV59_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325658.1"
                     /translation="MEHRIAYGRTSMLRKLLASRWPPVVLGIAAGVVLAIRPLELFAD
                     GPFASSVELPILMTGLGVPLLCFAVSALRAVGVLDRWTSGGILVGAGAPQTWFALWTV
                     GLYAPTPTPTYLPLVGGLLCLAAALLLLAPPPGQRA"
     gene            complement(30766..31614)
                     /locus_tag="VFV59_07235"
     CDS             complement(30766..31614)
                     /locus_tag="VFV59_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325659.1"
                     /translation="MTTEAASTSPAVHPVGAERATVRMLKASVMLVLGAVLSACAVTS
                     ELLEAEPAPVPSDPVRFDSVDLANDRRSVRVDFIGGEEFDPDNPCSVAYHGTAEIVGD
                     ELEIGIYAEPHPKPLPPNTGCAGVGYPRSLALDLDEPFTGAAVRDLAGQVFLLAPPAR
                     LAQIGALPDGWELLREGNVAGSSTPRWERVWSPESDPLPAEGDSMLTLIQGLGGPVNT
                     AGGNRQPPVEVNGQPATLWLHQPSGEMVLVWQLGSDELALVGNLSDFSQDEFVALAES
                     VALPAD"
     gene            complement(31620..32249)
                     /locus_tag="VFV59_07240"
     CDS             complement(31620..32249)
                     /locus_tag="VFV59_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325660.1"
                     /translation="MEAVVVTREAPRALVWIALTGVVFVLLLLITTFAAAWISSQLGI
                     AEPEREHFALLNAILLSLSLLAAIPWIGRLLGEGRLVSPGVLVVLAFTLAAGSAYVIF
                     EDQRSGLVFDTDRALQEILVPVAIALLASTDLGRRSATTPRSRHAWSWAGLVGGLIVL
                     SLVALTAGKVIGNAMGMSRLDSPLTFAALALAAVYGITVILRRLLATSR"
     gene            complement(32270..32896)
                     /locus_tag="VFV59_07245"
     CDS             complement(32270..32896)
                     /locus_tag="VFV59_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325661.1"
                     /translation="MRNSMRLATAVAALAAAAFVTPQAALACSGPMPTREEAVAAATI
                     ILAGRVVSQPSEWTYELDVERVFRGPQAESIVIGDPAPSTAPICSHQLKIGDRVVVAL
                     QDPTDIGLFSSAVWYLLPDGTVGTLAPEPPAATHDELFGYLRLLPDTSMPAEQGSGSL
                     LGTLGAISLSASVALAVIRGGSVRRRSRRGPVASGHRAATDAAPSASR"
     gene            complement(33051..33626)
                     /locus_tag="VFV59_07250"
     CDS             complement(33051..33626)
                     /locus_tag="VFV59_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325662.1"
                     /translation="MWRPLRPVLVALAIGLLFGIVALMLSLNPPYSSGGGVHYTHFGG
                     DGFGFDYPDDWQVIAGPRHYGHHGPTVLAAVGIGDFDLGCTETSTSVSCGSAPEWTVP
                     DDGIVVAYHFSAHLPMPQATPSLAAGEEWVEVGGRDAILSRTSTSMRWHFPGAPEFIE
                     ARWGPAAAQTAPAQLQTVISSWHFDSPPPDG"
     gene            complement(33825..34121)
                     /locus_tag="VFV59_07255"
     CDS             complement(33825..34121)
                     /locus_tag="VFV59_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325663.1"
                     /translation="MTITIRNDWDKAAILEITEPPGDVPGGVLAVVAPLISVDPGQEE
                     AVRIRVSIDRWTLRFSNGDRGFLYSDDLLAPVAQDSGAYVHIDDQGRLALEDDP"
BASE COUNT         4998 a        12204 c        12170 g         4965 t
ORIGIN      
        1 ctcctccgcc ttgctggcca tggcgaggac cgcctcgagc cgctcgtgct ccgtgggtgt
       61 ccgcccggtg gtcggatcgg tggtgatgtc gctgacgctg aagatgccga actgcatgtg
      121 gtgctcctgt gccgcggccc gtcctgcagg ccgcgcacca aaggatagca caactcgttg
      181 catacgcaac tatctagccg gtgagcgcgc gccagccgcc accgaaccgg tccatcggcg
      241 cgccgggcgc gaacgccgtc gcgagcaggg cgaggccagc gagcaatccg accccgatgc
      301 cgacgacgtc cgacccgagc accagcggcg cgacggccac gccaaggccg accggcagct
      361 ggatgcgccg gaggacccgc aggaccgggc tggcggcgat ggcgccgcag gccgcggcga
      421 gcggacccag gacgtggaag gcatcggcca cggccgccgg cagcgtgagg atgccgggtg
      481 cggccatcag cgccaccccg attgccgctc cgagcagctg ccaccccatc agcccgctcc
      541 ctccccggcc cagaatgcgc gccacaccga tccgccgcgc tggtgcacgc gccggaggtg
      601 ctgcgcgctg gcgagcacct cgtcgaaggc gggtccgatc atcgcgatgc tgatggcggc
      661 cgacgcgagg cacagcgtgc accaggcgtc gaacatgacc ggctgcagga tgacgagcac
      721 gatgctcgtg gcgccgagcg gaccgacggc gaggccgaag aggatgacga tccacggcat
      781 cgtccgccac cgctcgcggc cgccgaccac gcccgaggcg acgtcggtga tgtagccgac
      841 agcgcccagc gccgcgtccg ggatgggcag cacgcgcgag acgcccgatg tgaggacggt
      901 tcggcttccg tcgccgaaga agggatcgaa cacgtccctg gtgaggccga actggaacgc
      961 ggcgagatac gaggcgatgc cgaggccgat cagggccagc acgaggatcg ggatccgctc
     1021 gccccagcgc gacgggttgt aggaccagcc gggcggggcc ccggcctcag ccacggcgca
     1081 gcccgagcgc cgccaggccg gcgacggcgg ccgtgccgag cgccgagagc accagcgtcg
     1141 gccggtgcgt gttcgcccac agctgccagc tggagtcgcg cgcccggtcg tcgaacacgc
     1201 cgtgcgcgcc atggtcgccc ccgcccggcc cgtccaccgg ctgccacagg ttgttggggc
     1261 ggttcgggtc ctcgggctcg tcgatctgct gcgacgcgta gccggtgcgc gccagaacgc
     1321 gatccgcgta gcccggcacc accttgttgc ccatgatcgc cttgagggcc ggccagccga
     1381 tgatcagctc gcgcgtcgca tcggggttgt cggcggccca ggcgatgccc tcggcgatca
     1441 cctcgggctg gaagatgggc ggcaccggct gcgccttatg cggcaggcgg ctcttcaccc
     1501 agcggaactg cggcgtgttc accgccggca gctggaccat ggtgaccttc acgttgctgc
     1561 gatcgtgctc cagctcggtc cgcagcgagt cgtgaaagcc ctgcatggcg tgcttggcgg
     1621 cgcagtaggc ggcctggagc ggaatgcccc ggtaggcgag tgccgagccc accagcacga
     1681 tcgtgccgcg gtcacggggc ttcatccgcc gcagcgcggc gagcgtgccc cacaccgcgc
     1741 cgaggtacgt gacgtcggtg acgcgccgga agtcgtcggg cgtcatctcg tcgatcggcg
     1801 aaaacacgct ggccatggcg ttgttgatcc agacgtcgat cggtcccagc tcgcgctcga
     1861 gcgtctcggc ggcggcctcc accgcgtcgg catcggccac gtcgaggggc agcaccaggg
     1921 ccgtgccccc ggccgcctcg acgtcacggc gcgcaccttc cagcccatcg gtaccgcgcg
     1981 cgatgagggc cacgcggtcc ccgcgtgctg cgaagatccg ggcgagcgcc cgacccactc
     2041 cggcggaggc accggtgatg acaacggtct gcggttgcgg catggggatc ggtctcctta
     2101 tcggcgcgtg acagcccgcc acatgacgcc gagggagagg ccgtagacga gatgcgcgac
     2161 gagcatggtg gacgcccggc cggggctgtc cctggtggga ggcggcagga tcccgagcgc
     2221 cgggatccag ccgaagtagc tgccggccca gatcgcgagg gcccacggca gcgcgagagc
     2281 gccggggatg gccgggcgca cgagctgcgc gagcaggccg aatccggcgc cgccggccgc
     2341 accgaaggcg aggtgcgcgg cgctggccag cgcgttctgc tgtccctcgc tggtgggcgt
     2401 gccggtcgcc ttgctgcccc gctcgacgat gcgctcgggc gggtgctcag ccatcagccc
     2461 gaggcggtcg gccacgagca tgacggcgct catggcgagc gtcgcgatca ggccggcgag
     2521 cgcgccgaga cccatcgcgt gcgacaacgg ccgggagtgg cgggtcatgg tggcgagctc
     2581 agcaaggcgt gtgccctcgc ccgccaggtc agccgccgtc gaccatcggc cgcacctcgc
     2641 cgatccagcg ctcgagggtc tcctcatcgt acggcgccgc catctcggcg atgaacgtgt
     2701 ggaacccgag ctccttgcgc cgatgcatct cctcggcgac ctgctcgggc gtgccgaccc
     2761 agaaggtgtc gtcgtcctcg acgtcggtca tcggcgtgcg gttgtgcgcc atctgcgcct
     2821 cccacacgcg ccgcgcctca gcctcggtgt cgcggatgag cggcttgcat ccggcggtga
     2881 actcgatctc ggccggatcg cgcccgacct cgtcgcagtg ccggcgcagc acctcgacct
     2941 tgcggcgcag gaagtccggc tcccccatcg cgttccacat gtcggcgtag cgggcgaccg
     3001 tgcgcagcgt cttcttctcc cccgagccgc cgatcatgat cgggagccgc tcctgcacgg
     3061 gcggcggcag cagccgcagg cgctcgaagg cgtaggtcga tccggggggc gaggtgacct
     3121 cctcaccagc gaacagcgcg cgcagggcgg ccacggcttc gtccagccga tccaggcgtt
     3181 cgccgaagcc ggagccgaag tcgatgccgt gctcggtgtg ctcgagctcg aaccaggcgc
     3241 cgccgatgcc ggcgatggcg cggccgccgc tgaggtggtc gatcgtggtc agcgtcttgg
     3301 cgaccagccc ggggttgcgg aaggtgttcg ccccgaccag caggccgagc cgcgctcgcc
     3361 gggtgacctt ggcccaggcc gccagcgtcg tgtagccctc gaagatcggc tggtagggat
     3421 cgccgaagat ggcgtagagg tggtcccatg tccacaggtg gtcgtagccc agctcgtcga
     3481 cgcgccgggc cgacgcctcg aactcgtccc agccgctgga ctggctccac agcagcacac
     3541 cgaggttcgt tgccatgcca gtgcctcctg tcctcggact accgagtctt cgtcactttc
     3601 tgcaggccgg cgggggcgtc cggatcaagt ccgagccgcc tggctgacgc ctcggccagc
     3661 aggtagccgg ggagcacgcc gacgattggt gtgagcgact cctgcaatcc ggtcgccaac
     3721 gacagatcgc gcggccgtgg ccgatcagcg ccgccttcgc cgatcccgat cacccacggc
     3781 tcggtcccgc ggcggccggc cgccgcgctg gccgcatcga tgctgctggc gaccggtccc
     3841 tccggtcgca ccacgagcat cggcacggcg tcgtcggcca gcaccatcgg gccgtgcagc
     3901 acgtcagcgg ccgagtacgc ctccgcgaag acgcgagcgg tctccttgag cttgagcccg
     3961 atctcgagcg ccgtggcgag attgaatccg cgcgagacga tgatggcgcg gccgaccgac
     4021 gcgatcgcgt ccacggctgc gccgccagcc tccggatcgg cgatccatcg ctccgcggcg
     4081 atgagcgtct gcgaggcgac ctcggggacg cgtcgcagct ggctcagcag cgcgccgtcc
     4141 tcggccaggg tggcgacgaa ggaggccgcg gcgacgagct gggtgacgta ggtcttggtc
     4201 gccggcacgg cgtcctcccg gccggcccgg cacagcacga cgcggtgcac cgcaccggcg
     4261 acgggcgacg cgggatcgtt cgtgatcgcc agggtcaccg ccccggcgtc gcgcgcggcc
     4321 cgcgcgacct ccaccacgtc ggtactctgg ccggactggg agatggcgat caccgcgccg
     4381 tccgcccatc gcagccgggc gccgtaggcg gtcagaaccg atggcgcggc caggccggtc
     4441 gggatgccga gaacggtctc gatcaggtag cgcgcgtaga cggcagcgtg gtcggagctg
     4501 ccccgcgcga cgatcgtgat ccacgcgggc cgctcggcac ggaagtcctc ggcggcggcg
     4561 gcgacctccg gccccacccg atcgagcagt gccgccacag tggccgggat gtcgtggatc
     4621 tcccggtcca tcgcgctggc ggcgcgcggc gcatcgcctg tcgtcattcc gggaagaacc
     4681 ggggcaacat cgcacggttc gcttccagga tcgcgtcgag caggagcgcc ggatccgtag
     4741 acggaccgac caacgggttg gcggcgagcg ctcggagggc atcaccccgg tcgccactga
     4801 gggccgcgcc gatgaccagg cgctcgaacg ccttgacgga ccgcaccagc tcgagcatct
     4861 ccgggctgag gggatccacg gaacgggggt gcgccccgtc gcgatcgatc cgcgacgtga
     4921 cctcgacgac cgcgtcggcc ggcacgcctg ggacggcgcc accgttccgc acgtcgacga
     4981 cctggaggtc gccgcttcca tcgtgcaggg atgccagcag ctgggcggcg gcctcggagt
     5041 agtacgcgcc accgcggtgc tcgagcagct cgggcttggt gtcgaccgac gggtcgcggt
     5101 agagctcgag gagcttggcc tcgatatcca tcacctcctg ggcacgcgag gtcttggcac
     5161 gctgctccgc gagcacatcc tcgaaccgct cgtagtaccg caggtagtag gacgggatcg
     5221 ctcgctgcgc gcgcatgcgc tccaccggga tgccgatgtc gcgctccagg tcggagccga
     5281 tgcgctcgat gacctcgtcg atgcgctcga cgccccggac gcggacgccg cggatccagc
     5341 tgaggtggtt gagtccgaca tggtcgagct cgatctcgtc ggggccgacg tcgaacagcc
     5401 gtgcgaaccg ccgctggaag ccgatcgcga cgttgcacag accgatggcc cgatgaccgg
     5461 cgtcgaggag cgcctgggtg acgatcccga ccgggttggt gaagtcgatg atccaggcgc
     5521 cgggccgcga gcgccgtttg acctcgtcgg cgatgtccag gacgaccggc accgtgcgca
     5581 gcgccttggc gaacccgccg gcgccggtgg tctcctggcc gatggtcccg aagcgcagcg
     5641 gaagcgtctc gtcgacgagc cgcgccgcct gcccgccgac gcgcagctgg agcagcacga
     5701 agtcggcgcc gtcgatggct tcctcgatgc gcccggtggt cgtgagccgc ccgggccact
     5761 cgaggcggcg cagcatccgc tccgccattc ctcccagcac cccgagacgt tcggcgtcga
     5821 cgtcctcgag gaccagctcg tccagcggga gccgctcgga ccggctggcg aggccctcga
     5881 ccagctccgg cgtgtacgtc gatccggcgc cgatcacggc gaccttgagg cccattccac
     5941 cgcctccgga ggctgcggac tcttgacaca ttgcttataa ccttatgagg aaccgcgggt
     6001 caagcgctcc gccaccgccg ttcgttggag ggatggatgg cacagttccg gctcgagcac
     6061 gggccgatcc cgctgcacca ccaggtgtac ctcgacgtcc gacgggcgct ggccggtggc
     6121 gagtggtccg ccggcgatcg tctgccgacc gagcgcgagc tggccacgcg ctacggctgc
     6181 tccctcatca cggtgcgacg ggcgctggac gagctcgccc gggaaggacg gatcgagcgc
     6241 acgcgcggtc gcgggacgtt cgtcctggcc agccgcatcg agcacgactt cgccgggcag
     6301 ctgagcttca gcgaggacat gcggcggcgc gggctgcgac cgtcgacgcg gctggtcgag
     6361 cggcatgtcg agccggcgac ggacctcgtg gcggaggcgc tcggcatcgc tccggggagc
     6421 ccggtcgtct acgtcgagcg cgttcgcatg gccgacgacg agccgttgct gctcgagcag
     6481 gcgcgcctct cggcggagcg attcccgacg ctgctgcagg ccgacctgga gcgcgagtcg
     6541 ctgtacgacc tcctcctgca ccggcacggc acacgggtcg tgcgctcatc ggagacgatc
     6601 gaaccggttc gcgtgccgcg ccgcgaagcc gacctcctcg ccgtgcgtcc gggcagcctg
     6661 gcgctcctcg tcatcgggac cgccgcgacc gtcgccgggc cgcccgtcga gtacacccgc
     6721 agcttcatca gaaccgaccg caccaggtat cggctcgatc gcgtgattca gcgccccgga
     6781 tgggctcgaa gcttcgagac gggcgtcagg agcgcggcgg catcgccatc ggcgcctgga
     6841 ggcaagaagg gaggggcacc catcgcggag cgagtttccc gctgagagaa cgcaacgtgc
     6901 cttcacaacg aggtgacgaa ccgtggtcaa gcgtcgactt gtgatcctgc tcccgctcct
     6961 ggcgctcgtg gtctccgcct gtggcggaca gacgacgacg acgtcgcctg gcgagagtgc
     7021 gagcggatcg gcagcatcgg acggagcctc cggcgacgtg gtcgaggttc gctggtactg
     7081 ctgcctgggc accggcgagg acgccgagtc gcagctgccg gtggagaacc gaatcgtcga
     7141 ggagttcaac gcgtctcacc cgaacatcca cctgacgttc gaggtcgtga cgtacgacga
     7201 ggcgcgggac acgctggcga cgcagctcgc ctcgggcaac ccgccggata tcgtgggacc
     7261 ggtcggcatc ggtggcgccg aagccttcca cggcgaatgg ctcgacctga cgcccctcat
     7321 cgaggagtcc ggattcgacc tgggccgcta cgagcaggct gcggtcgagc tctaccagtc
     7381 cggtggcgag ggccaggtcg gcattccgtt cgcggcctac ccctcggtca tgttctaccg
     7441 caagagcctg ttcgaggagg ccggcctcaa cgagccaccg cacgagtacg gcgagccgta
     7501 cgtctggccc gatggcaccg aagcggagtg gaactacgac acggtgcgcg agctcgccct
     7561 gcgcctgacc gtcgacgaga acggcctgga cgccacgcag gacggcttcg accccaccgc
     7621 catcgtgcag tacggctacg agccgcagcg cgacgacatc cgcggtctgg gtgcctactt
     7681 cggggccggc tccatcgcgt cggaggacgg tgagaccgcg cagatccccg agccgtgggc
     7741 ggccgcgtgg aagtactggt acgacggcat gtggaacgac gggttcatcc tgaccggccc
     7801 cgactacgac tccgacgagt gggcagggca ggtcgactac gcgttcttca ccggccgcgt
     7861 cgccatgagc acgaacttcc tgtggtccac ctacggcgtg gccgagggtg gagacgactg
     7921 ggacgtcgcc gcggtgccgt cgtacgaagg aacgacgacc tcgccgctca acgccgacac
     7981 cttccggatc atgaaggact ccgcgcaccc ggaggaggcg ttcgaggtcc tgcgctactt
     8041 cctggaggat gcctcagccg atctgctgaa catctacgcc ggcatgccgg ccgttcccga
     8101 ggagcaggac gccttcttcg agacgtacca ggagcagttc ccgaacgagg tcgactggca
     8161 ggtcgtcaag gacagcctgc agtacgccga cgtcccgaac tgggagggct acatgcccgc
     8221 ctacaacgag acgctggacc tgctgctgac ctacggcgac aagtgggcga ccaccgacgg
     8281 cctggacatg gacgaggaga tcgccacgct ccaggacgag atccaggcga tctgggatcg
     8341 ctaggctgag cgcgacggac cgatgacggc tgccccgcca tcgcgccccg tgcgcatgac
     8401 acccctggcg cgccgagagg cgcgctgggg gtacctcttc atcctgccgt gggtcatcgg
     8461 cttcgcggcc ttcacggccc tcccgatgct ggccacgctc gggttcacct tcaccaacct
     8521 gacgctcacg caggacgagc cgctcacgtt cgtcgggatc gacaactacc ggcacctctt
     8581 ctccgacccg cagatgtggc ggtccctcgg cgtcacgctg cggttcgccg tgctctggtt
     8641 gccgacggtg atcctggctc cgttcgcggt ggctctgctg ctcaacgcca agagcctccg
     8701 cgggagccgc ttcttccgcg tcctcttctt cctgccctac gtcgtgccct tcgtcgctgg
     8761 agtcttcctc tggcagagca tgctcgccaa cgacggatgg gtgaacgacg tcctccgctt
     8821 cctggggctc gcgcagccgc cgaactggct gttcgatcgc gactggatct acccggccct
     8881 ggtcgtcatc ggcatctggg gcatcggcgc cgggatcatc atcaacctcg ccggtctgca
     8941 gggcatcccg gccgagctgt acgacgccgc ccagatcgac ggcgcgggct ggtgggcgca
     9001 gctgcgcaac gtgaccatcc ccttcatgac gccgatcatc ttctactcgc tggtgctcgg
     9061 catcgtcgag gtgatgcagt acttcctggt gccgctggtg ctgaagagcg gcaccggtga
     9121 gcccggcggc gccacgctct tctacaacct ctacctctac aagaacttct tcaccttcca
     9181 gaacatggcc tatggcgcca ccctggcctg gttcctgttc gtgatcatcc tggcggcgac
     9241 gctggcgctc ttctggtccg cgcggcgctg ggtgttctac gcgggcgagc gctgacatgc
     9301 ggtcaccgaa ggcgctggcg gtcacgctct tcacggtcct gctggcgggc gtgttcctct
     9361 ctccattcct gcgctccgcg atgatcgcgc tgtcgacgcg cgaacagctc gggcaggcca
     9421 acgcaccgat ctggccggcg aacccggcca cgttcgagta ccagggccgc gagatggtga
     9481 tctacgaggt gccgttcgat gacggcacgc gcgacctggc gctcctgcgt ccgggccgaa
     9541 cgagcagcga gttcatcgac cccgccgatc ccgagaccgt gattccctgg gagggctcgt
     9601 ggcgcgcgct cgagcgcagc tggacggtcg ccccgacgtg gtccaacttc agcgacgtct
     9661 gggtcctgat cgactatccg agggtgctgt tcaatacgat tgcgctggcc gtcatcggca
     9721 ccatcgggac ggtgctgtcc tgcaccctcg tggcctacgg gttcgctcgc ttccgcttcc
     9781 ccggtcgcgg catgctgttc acgctgctca tcgcgaccat cttcctgccg gccgccgtca
     9841 cgctcatccc gacgtacacg gtgttcgtca agctcggatg ggtcggcacc tggctgccga
     9901 tcctggttcc cgccttcttc gccaacgcgt acgacgtgtt cctcctgagg cagtacctga
     9961 tgaccatccc gcgcgatctc gacgaggctg cctcgatcga cggcgcgggc cccttccgta
    10021 tcctgtggtc ggtcatcctg ccgcaggcgt ggccggccgt cgtgacggtc ggcatcttca
    10081 ccttcgtgta cacctggaac gacttcttcg ggccactcat ctacctctcg acgcggcctg
    10141 acctccagcc gatctcggtc gccctcgcgc gcttcaacgg gctgtactcg agcaatcccg
    10201 ccctgatcca ggccgggacg ctgatgacgc tcatcctgcc ggtcctgctg ttcatcgtgt
    10261 tccagcgctt cttcatccgc gggatcgcgc tcaccggttc cggggtcgag cgatgagcgc
    10321 cgggttccgg gtggcgctga cgttcgacgc cgagcaccct gatcggccga acgacggcct
    10381 cggcgcggcc cgcatcatcg agacgctcgc cgctgcccag acaacgggga cgttcttcgt
    10441 gcagggccga tgggcagagg cgaacccgac gatggcgcgc cgcatcgtga cggacgggca
    10501 cctggtgggc agccactcgc agtaccacgc ccgcatgccg ctcttcacgc cacgaggatt
    10561 cgccaccgac gtgcgcgccg ccgagggcgc catccggcgc gtcgcccgcg tcgatccgcg
    10621 gccatggttc cggcttccgt tcggctcggg ggcgacctcg cccgaggtga tcgcggcgct
    10681 tgcggctctc gggtatcgcc acgtcggctg gcacgtcgac gtgaaggagt ggcgtcggcg
    10741 ggagacgatc gccagcgtac gcgaccgagc cctcgagctg gtccgcgcac acggcgacgg
    10801 cgcgatcgtc ctgctgcaca cctggcccga cccggtgggc ctcgcccttc cggcgctgat
    10861 cgaacgactc gcctccgacg gcgcgcggtt cgttcgcgtc gacgagctcg gcctggggga
    10921 cgggctcgag ccggtcgccg aaccccgccc ggccgacgcc accacacgcc gatgagcgcg
    10981 ccggtcgcga tcctcggcgt cgacggcggg aattcgaagt cggagatcgt cctcgcggcg
    11041 cgcgatggaa cggtcctcgc acgccggcgc gggccgacca tctcgcacca gtcgatcgcg
    11101 ccgggacatg gtctgacgcc tgcgcagcga gcgtcgctgg gcatggagcg gctcgcgcag
    11161 ctcacccggg acgcggctcg tgaggccggg ctggttcccg acgcgaaccc gctcgcggac
    11221 gtggcggtgc tctgcctggc cggcgccgac ctgccgtccg acgttcggct gctgggatcg
    11281 gccctggcgg cccagcacct gctggagcga ccggtgatcc tcaacgactc gttcgcaccc
    11341 ctgcgcgcgg ggacggagcg ccactacggc atctcgctga tctgcggcgc cggcatcaac
    11401 gcggccgcga tcgcgcccga cgggcggacc gctggcttcc ccgggttggg gacgatctcc
    11461 ggcgattggg gcggcgcgtc gggccttggc atggccgggt tggtcgccgc cgtccgcgcg
    11521 cgagacgggc gagggccgcg cacgctgctc gaggagctcg tccctcgtca cttcggtgtc
    11581 atgcgcccag cgacggtcac ccgacggctg tacgaaggag agatcgacga ggagcgcgtt
    11641 cgcgagctcg cgccggtcgt gttcgaggcg gccatcggcg gcgatcgcgt ggcccgcggg
    11701 atcgtcgatc atctggctga cgagctggcg acgatggcaa tcgcgctggc tcgccggctg
    11761 gggatgacgc gcaccgcgtt cgatgtggtg ctcgccgggg gcatcttccg cacccgggac
    11821 cgcgggctgt tcgatcgcat cggcgagcgg gtggcgaccg cgctcccgca cgcaaccctg
    11881 cgccgcctcg acgcaccgcc agtcctcggc gcggccctgc tcgcgctcga ccatctgcca
    11941 ggggccgaac cgggggccga ggagcgcctg cgggccggcg tggtcgcatt ggacccgcca
    12001 ccggacgccg ctgcctgatg tcgcgcggcc acgagcggtg gcggccggtc gcgggatacc
    12061 tggcgcagta ctccgcggtc ggcgcgctct acccgttcct gccggtctac tacgcgaccc
    12121 tcggcttcgg cctgcaggcc atcggcctcc tgggcgcggc gttcgccgtc gccgcgctgg
    12181 tcggggcacc gctgtggggc gcgacggccg accacctcgg agccgcgcgt cccgtcctct
    12241 cggtggcggc actggccgcg tcggtcagcg cggcgggact cgcgctgtcg cagggcagcg
    12301 tgcccatcgt gctgagcgcc gtcgcgctgt cactgatgat ggccggcatc ggcccgatcc
    12361 tggacgcgcg cgcgatggag atcgtcggcg cgggacgggg cagctacggg cgcatgcgcg
    12421 tctgggggtc cctgtcgttc atcggcgcgg tcctgctcac gggttggctg gtcgaggcgt
    12481 tcggcatcag caccatgttc gccgtgctgg tgattgccct tgtggcgacg gcagtggtgg
    12541 gcttcggcat tcgatcgcgc ccggccgtcg ccccgcttcc cggacgcgcg gcactcgccg
    12601 ccgtggcgcg cacgccgatg atcatggcct tcctcggcgt ggttctcctg gcatggacgt
    12661 catcgaccgc gatcaacgcc ttcttctcga tccacctcgt cgacatcggg gcaccggaat
    12721 cgctggtcgg agcctcgtgg gcactggggg cggtggtgga ggtaccgatc atgatcgcgt
    12781 tcccgtccct ccagcggcgc atcggcctgg agcggctcct catcgccggc gcggcgctct
    12841 tcgccctgcg cggcgtcgcg gtcgtcctca ccggtgaccc actgctcacg acgctgacga
    12901 tgctcattca cggcgcggcc ttcgcgctcg tgctcgtcgg cggcgtcgtc tacgtctcca
    12961 cgcacgctcc tcggggcgca gccgccagtg cgcagggcat gctcgccgcg acggtgttcg
    13021 ggttggcaca ggtcctgggt cccgggatcg gcggcgtggt cgcgcggtcg ggtggcctgc
    13081 ccacgctctt cgcgatcgcc acggcaggca gccttgccgc aacggtggcc ctcgcggcgg
    13141 tgatcggccg cgagcagccg ggggtgccgg cgggggtgcg acgagaggga tcagcatcgt
    13201 gaagttcggg ctgcgcatcg acgacttcgg ctggccgggc ggaggacgcc ggatggcgga
    13261 gcacctcgcg agcatcgggc gtcgcgcgga ggacgcgggg ttcgactcca tctgggtctg
    13321 ggaccacttc gtccagctcc gtcgctggga ggagccgctg ctcgagggct ggatggcgct
    13381 cgcctatctc gccggcgtga cgcggcgcgt gagactcgga acgcatgtca ccggcgtgac
    13441 ccaccgcgca ccggcggtgc tcgtcaagca ggcgacgacg ctggacgtcc tgtccggcgg
    13501 acgtgcctac ttcggggtgg gcgccgggtg gaacgagcgc gagcacgacg cctttgggat
    13561 tccgttcccg ccgctgcgag agcggttcga gctgctcgag gacacgctgc agctggccct
    13621 ccgcgcctgg agcggcgaac gcacgccatt cgtcggcagg cacgtccggc tgaccgagca
    13681 gatcgacgtg ccgcaaccca tgcagcgacc ccacccgccc atcctcatcg gcggcgtcgg
    13741 ggaacggcga accctgaagc tcgtggcgca gtacgcgcag gccaccgacc tgtggctcag
    13801 cgatcccgac cagatccggc acaagctcga cgtgctgcgg cgccactgcg acgccgtggg
    13861 acgcgacccc gaccacatcg agcggtacgc gcgcgtcgat ctgacgatgc cgcatgggga
    13921 ggaaggcgag gtcgcgcccg cgccgatcct gctcgaccga attgcggcca ccgccgcggc
    13981 cggtgccgac gccatcgtcg tccccctgcc ccgcatggcc gagccgggcg tcatcgagca
    14041 gttcggcagt gaggtcatca cgccagcgcg cggcatcgcc cggccgcctg gctgaccgcg
    14101 gccctaccgg tggtacggca cgtcggtgac gatggtgttg cggcggaaga agaggcggaa
    14161 cttgagccgg agggccgtct ggttgtggag caggccttcc caccagtggc gcgggaccac
    14221 ttcggcgacc accaccagca ccggccgtgt cgggtcctgg cgctgcagcg cgtcgaggta
    14281 gcgcagcagc ggctggagca gtgcccggta cggtgactcg acgatcacca gctcccccgc
    14341 ccccaccagc tcgggccact ggcgccgaag gctctcggcc tcggcggcgt cgttcgtgat
    14401 gtggacggcc agcgcctccg gatcgagcgc gtgggcgacc gccaccgcct gcagggcggg
    14461 ctggtcgagc ctggccaccg gcacgacgac gatcggtcgg cggcgaacgg ccagctgggc
    14521 cgtcggtgcg tcgagcgcca ggctggagtt catgtgctgg tagtgcgtcc gaatccccca
    14581 cagcagcagg acgacgagcg ggatcagcac catcacgatc cagccgccct gcgtgaactt
    14641 gctcacgccg acgatcacaa cgatcacgcc ggtcgtcgcc gccccgagcg cgttgatcgc
    14701 catcgagtgg gtccagcccg gctcgcgacg gcgccaccat cggaccacca tcccggactg
    14761 ggagagcgtg aaggcgatga acaccccgag cgtatagagc gggatcagcg cggtgacgct
    14821 cgccccgaag gccacgagca ggacgaccgc caggctggcc agcgccacga tcccagtcga
    14881 gaaggccagg cgatcgcctc gatagccgaa ctggcgcggc atgaagccgt cgcgggccag
    14941 gaacgacgag agacgcggga agtccgcgaa gctcgtgttg gcggccaggg tcagcaccaa
    15001 cgcggtggcg acctgcacca gcaccaggta ccacccgttg ccgacgatgt gccgggtgac
    15061 caggctcagg accgtctgct cctcggacgg gtcgggcacg atgccgatgc tgctggtcag
    15121 gatgctgatg cccaggaaca gcgacccgaa cagcgcggcc gcccaggtga gcgtcgttcg
    15181 cgcgttgcgc cactccggtg gcttgaaggc cggcacgccg tccgagatcg cctcgacgcc
    15241 ggtgagcgcc acggcacccg acgagaacgc gcgcagcacc aggaggatgc cgaggggctg
    15301 gaccgccgcc tcggcctcga gccactcccg cggcggctgg tacggcgcca gcgttccggt
    15361 cgccatctgc cacaggccgt accccagcat gccgaagacg gccacgaggt acacgtaggt
    15421 cggcgccatg aagattgagc cggattcgcg gatgccacgc aggttgccga tcgtcagcag
    15481 cacgacgatg cccacggcga ggaggacgcg gacgtcgaag agtccgggag cgatggaggt
    15541 gatggccgcg acgccggcgg agacggagac ggcgaccgtg agggtgtagt cgatgagcag
    15601 cgccgccgcc gccaccagtc ccgcaggcgc cccgaggttg tcgcccgcga cgatgtagct
    15661 gctggcaccg ttcgggtagg ccttgatcgt ctgccgatac gacgtggcga cgatcgccag
    15721 gaccgcgacg atgaccaggg tcagcggcac gaccatgctc aacgccccga tgccggccag
    15781 gacgacgatg cgcatcatct cctcggtcgc gtaggccgac gagctgatgt tgtccgagct
    15841 gaacacggcc agcgccttga ccttggtcag gcgctcgtgc tcctccatct cgctggagat
    15901 ggggcgcccg aagaggaaga tccgcgcggc gcgccaggca gcaccgatgc gcccgccggc
    15961 gcgcagggtc tcctccgtgg cgaccaggtg tcctgcctgg gctcgctgga actcgtggtc
    16021 gaagggccgc tcgatgcgga cgtacacgtc acccgcgcgc cggccacgtt ttcgctcacg
    16081 gacctcgagg tacgggtgga cgtcggtcac cgccgcatcg ccccgtcccg tgacaggcgc
    16141 acctccagcg caccgttcgc gatcgcgacc gaggcgcgac agccgtactc ggccgcgatt
    16201 ccccggacgg aggcgaggag cgcggtggcg ggcacgccgt ccgggatgtg gctgacgaca
    16261 tcgatggccg tgacctcggc atcggtcccc gccagtgcct ggcaggcggt gcgaagcatc
    16321 gaaccgacgc gggcgggcag ccagggaagc gccggacgtg gtgtggaatc aggctcgatc
    16381 acccgtccac catgcgccgc ctcacctcaa cgaggtgtca acgccacagc ggaaggcctc
    16441 aacgttggct caacatcgcg gcggccatcg ccgctcagcc ctcggcgtcg acctccagca
    16501 gccggtagcc gacgcccggc tcggtgcgca ggtaccgcgg attggccgga tcggcctcca
    16561 gcttcttgcg gagtcgcgcc atgtagacgt gcaggtagtg gctctcggac ccgtactccg
    16621 gaccccacac ccggcgcagc agcgcgcgat cggtcaggac gcgcccggcc tggtccacga
    16681 gcgccgtgag cagcgcgtac tccgtgggcg tcagctgcac cggtagcgcg tcgaccgtca
    16741 cctcgcgtcg ctcgaggtcg accgccaggc cgccgctgcg gaagatcgca tcggttccgc
    16801 ggccaccccg agcggcatgt cgcagcgcca cgcgcacccg cgcgagcagc tcatcgacac
    16861 cgaagggctt ggtcaggtag tcgtcggccc ccatctccag cgcgcggacc ttgtcgcgct
    16921 ccgcgccgcg cacggagagg acgatgatgg gcgtgctcgt ccgatcgcgc gtctgcgcga
    16981 tcacctcgaa gccgtcgacg cccgggaggc cgaggtcgac gagcatcagg tccggctgga
    17041 agcgaggtgc gacgcccagc gcctcttcgc cgctgctcac ggcgcgcacg acgaagccgt
    17101 ggccggtcag cagccgctcc accgtgcgcc ggatcgcctc ctcgtcgtcg agcacgagca
    17161 cgcgagcccc cgtggacgct gcctcagtca tggcatcgcc tcccgggtcg ggatgacgaa
    17221 gcagaagcgc gaccctcctg cggccgcgct ctccgcccag atccggccgc ggtgcgcctc
    17281 gaccaggccg cgagccacgg caaggcccag gccggtgccc cgccgggtca caccgcgaac
    17341 gcggtggaag ggctcgaaga tccgtcgcag ctcgtccacg gggatgcctg gaccctcgtc
    17401 ctccacgcac acgcgaagct cgccaccgtc gacaaccgcg cggacggtga cggtcgtgcc
    17461 gggtggcgaa tggcgcacgg cgttctcgac caggttcgtc aggacctggt cgatctccga
    17521 gtagtcgagt gggaccggcg gcagctcgtc cgggaggtcg agcaccagcc gatgcgccgc
    17581 cgtcacgccg gccatgcgtg ccagcacgtc acggacgagg gcgaccggct cgtaccaggc
    17641 gacgtcgggg tgcaacgagc ccgcccgaat gcggctcagg tcgagcaggt tccccacgat
    17701 ccggtccagg cgtcgtgcct cctgctcgat cgcggccgcg aactcggctc gttcctgcgt
    17761 cgaccattgc accgcggcct gtcgcaggct gccggccgac gcgatgatcg aggccagcgg
    17821 cgtgcggaga tcgtgggaga cggcgtcgag cagcgccgac ttggcctcgt cggatcggcg
    17881 cagcacctcc gcctccgtcg cctccgcgcg cagccgcgac cgctcctgcg ccgcccacag
    17941 ctgcggagcc accccggcca gcagccgggc ggcggcgccg tcgaagccac ggcggttgct
    18001 cgtggccagg gtgatgtggc cggcctcctc accgcctgcg ccgcggatgg gagcacggcc
    18061 cacgcggtgc gccatcgcca tcgcggccag ccgtcggtgc ggtggcgcca gccggatcca
    18121 gcgcgcctcc gatcccccat ccgtggccag gacgtcgatc gtgcggtcgg tgttgtcgag
    18181 ggcctccagc gcgtcggcgt cgacgctgga cgcggcccgc gcgccgccga gctcgacctg
    18241 gaccgcatcc accccgagct cggcacggag ccgatcggcc acggagcgca gcgtccgctc
    18301 gagtgtcggc tccgtcaacg cgatggcgat gtcggtcagc acccgagcct gccgctcgtg
    18361 ctcctcggcc tcctcggccc gccgcttctg cccggcggcg agctgcccgg tcacgacggc
    18421 cgtgagcagg aagaggagga gcaggatcca gtcgtcgggg tcggcgacgc tgagcgtgtg
    18481 acgcggctcg gtgaggaacc agttgaagct gaagaacgcc agcagggcgc tgaagatcgc
    18541 gaccgcccgc ccggcggcca cggccgcgac cagcaccacg atgaggtaga gcatcgaggc
    18601 gttccccagg tccgggcggc cggccagggc gatgcccagg gtcaccatgg cgacgcccgc
    18661 ggtggccagg aggtacggaa ggacgaccgc cactggtgcc ggcagcatcg gtcgcctcca
    18721 cgtcccgagg cgccgagcgc ctccttcccg gagcgtcacg ggagcgatgc tacgtccgcc
    18781 gtcccggttg cgccgtgccg atcggccctt ccggcgctgg ccgcggcgcc cgaagactgc
    18841 ggccatgcgc gagtacgtgg acggcaacgc ggccatcgtg cgcggcgcgg tcgccgccgg
    18901 ctgcacgttc ttcgccggct atcccatcac ccccgcctcg ggaatcctga tggcgatgat
    18961 gcgcgagctg ccggcgcgcg gcggcgtcgc catccagggc gaggacgaga tcgcgtccat
    19021 cggcatgtgc atcggcgcgg ccatggccgg gtcgcgtgcc atgacggcga ccagcggtcc
    19081 gggcatgagc ctgatgggcg agaacctggg cctggccatc atgggcgaag tgcccctggt
    19141 cgtggtggac gtgcagcgcc tggggcccgc caccggcggc gccacgaccg tcgggcaggg
    19201 cgacgtccag ttcgcccgct ggggcacgcc cggtgggttc ccgacgatcg cgctggcccc
    19261 gaccgacgcg gccgatgcct accgcctcac catccgtgcc ttcgagctgg cggaccgatt
    19321 tcgctgcccc gtcgtgctcc tgaccgacaa ggaggtcgcc accaccctga ccacggtcga
    19381 cgcggcggcc ttcgcgccgg tggccgtggt ggagcgtgag ctggcgacgg ccggtgcgcc
    19441 gtaccgatac gccccggcgg cggacgtgcc ggcactgcgc cgctacggag ccggcgacgc
    19501 cttccgcttc accggatcca cccatgacga gcgcgccatg atcaccaagg accgacccac
    19561 cgtcgatgcc ctcaaccggc acctggcggc caaggtggag aagcacgtcg aggagctcac
    19621 gctcgtcgag ctcgactggc agcccggcgc cagaacgctg gtggtctcgt acggcgtcac
    19681 ggccggcgcg gcgcgagcgg ccgtggaagc cgcccgccag cgcggccggg ccgttgcctc
    19741 cctggtgatc cgcagcctct ggcccgtgcc cgaggcggcc atccgcgcgg cactgggcgg
    19801 gatcgagcgc gtcatcgtcc cggagctcaa cctcgggcag taccggcgcg agatcgagcg
    19861 tctcacgggg gaccgcgagg tgatcggcgt caaccgggtg gacggcgatc tcatcggcct
    19921 cgaggagatc ctggaggccg tcggatgacc gtcgtgtcgg gcgatctcgc caccgacctc
    19981 accaccctgg agacgttccg caacgccctc ccctacccgt tctgccccgg atgcgggcac
    20041 ggcggcgtcc tggatcggct ggacgaggcg ctccgccggc tcggacgcga tccggcgcgg
    20101 acggtgctcg tcagcgacat cgggtgctcc gggctgtcgg accagtactt cgtcacgaac
    20161 gccttccacg ggctgcacgg gcgaagcatc acctacgcca gcggcatcaa gctcgccgat
    20221 cccgcgctcg aggtgatcgt cctgatgggc gacgggagtg ccgggatcgg cggggcgcac
    20281 ctgctggcgg cggcgcggcg gaacgtcggg atcacggtcg tcgtgatgaa caacctcaac
    20341 ttcggcatga ccggcggcca gcactcgacc acgacgccat cgggagccgt gacctcgacg
    20401 acgcccggag gcaacctcga gcagccgctc gacctgtgcg ccacggtcgc ggtcaacggt
    20461 gcggcgtacg cgcaccgcgc gacgcagtgg gacgacgacc tggccgatcg cctggtcgag
    20521 gcgatcgaga ccccgggctt cgccctgctc gatgtctggg agctgtgcac cgcctacttc
    20581 gtcaagggca accgcctctc gcgacgcggg atggaggcgc tcatggagga gcgcgggttc
    20641 cggcgcggga cggtggtcca tcgcgcggtc gacgagtacg cggccgccta ccgccacgcc
    20701 caccaggccg atgcgcctgc ggcgcctccc gcaccgatcg cgatcgacca cgctgcgacg
    20761 ctggatcgcc cgtatggagt cgtcgtcgcc ggatcggccg ggggccgtgt tcggtcagcc
    20821 gcccggctga tcgcggaggc ggccgtgcgg tgcgggctgt gggccaccca gcgggacgac
    20881 tacccggtca cggtccaggc cgggcacagc gtgaccgagc tgctgttgag cccggcgccc
    20941 cagcccctcg ctgccctcga gaccccggag ctgatggtcg tgctgtcgga agacggccgc
    21001 cggcgcgtgt cgtccaccct gcgcaggttg agggcggacg cgaccgtcgt caccgtcgcc
    21061 ggcgtggcgg cacccgagac gccggcgcgc gtcgtggtcg cggatcccgg acggctggca
    21121 cgcggcgacg ccacgctcgc ggtgctggtg acggccatca cggccctcgg cgtgcttccg
    21181 gcgcaggcga tccgggccgc ggcatccgcc ggggcgcatg cgagcgccaa tctcgcggcc
    21241 atcgaggcgg ccacatcact gccgctcggc tgacgcgccg ctgaccttcg gcactggcgc
    21301 cggtccgcga cgcgacgtct gctgagacga gacgaaagga ggcactcgtc atgcgcgagg
    21361 agcatccgca cggtcgtccg gtggaggacc gcgccttcga ggccgtcgag atcgcggcgg
    21421 gagtggcggt tggcgccggg ctcggcgtcg cggtcgcggg tccggttggt gcggccgtcg
    21481 gcggcctcgt cggggccgcc gtcggcgcga tcggtggcga ggcgctcgag cgcgccgagg
    21541 ggcgcgcggc ggagacgacc gacgcgacgg cggtcgtgga ctgagccgcg cgggtcaggc
    21601 caggcggcga gctgccgcgc gaagcgcggc aagcacatcg gtccgctcgt gcgtgccgat
    21661 cagcagctca ggctgcggcc ggaagccggc gatgcaccgg cgcagctcac gcagctgagc
    21721 cggcggagcg tcctcgaccc ggaccgcgtc cgggagggtc gacagcgccg caagcgtgcg
    21781 cagggcgacg gccacgcgac ccaggggaag gacgacctcg atcccatcgg ccgtggactc
    21841 ggccgacacg gtggtgccga gctcgccgag acggtcgtag gcgcgccgcg cacgcagcga
    21901 gacgtcctcg accgggtggg gcccgtcgat ccgccagatg aaggtcgctc gctggcggat
    21961 cagcgccatg ctgcccaccg gtcgccactg gtcgggatgc agtccccacg ccgacaggaa
    22021 gaggtgctcg tagtcgctgt cggctccccc cagggtctcg gcggggcgcg tgcctcgtcg
    22081 tcgcatctgt tcgcacggta cccgagcgag cccgccggcg gagcctcacg tccgtaccgg
    22141 ttgcccgggg tacatcggtt cggccggcgg ttcggccggg cggttggccc gtgtgcgccg
    22201 ggcagtgcca tgccgatgtc tcgacgatcg agacccggcc tcgaggaggt gtctcatgac
    22261 ggtcagtgag ggagcggtga cgctgctgtg ggtcaacgcc gatgaggcgg tggcctggcg
    22321 gtggcaggag ggccacgagc agctgctcgg gcgcatggag tcggaggtgc cgacgcatca
    22381 ccgatcgatg ggccacgtcc gtcgcgcgtc cggtgatcgg cacgggggca gcgggccgcg
    22441 gtcggcgcag gagggtcatc ggctggagca cctgcgcgcg ttcctcgcgg aggtccgggc
    22501 cgcgcttccg ccggacgacg atctgctgct gctgggcgac gggaccgtgg tggaccagct
    22561 cggcgcgcgg atccgggagg aggacacgca ccaccgacgg acgcggacgg tcgaggtgca
    22621 ccacgccgag cgcatgacgg accgccagct catcgcccgc ctgcgcgggt tcgccgcgga
    22681 cccgccgcga cgcgtcctcg cccgccgctg accacggccg gcggtcaggc ggggaggcgc
    22741 acctcgaagc gcgctccttg cgagccctgg cggttcgcgg cgcgcacccg tccgccgtgg
    22801 cgctcgacga tccaggcaac gatcgccagg cccagcccgg tgccacccgc cggcgctccg
    22861 gacgctcgcc agaatcgatc gaagacgtgg ggcaggtccg cgtcgcggat gcccgcgccc
    22921 tcgtcctcca ccagcaggtg cgctccgcgg ccaccccgca ccgtgacggt gacggtgccg
    22981 cccgtcggac tgtgccgcac ggcgttgtcg gccaggttga cgacgacctg gcgaagccga
    23041 tccggatccc ccaccagcgg cgccggggcc gcgtccagcc ggagccgcac gccgacctgg
    23101 tccgccacgg gacggacgga gccgagcgcc tcgccggcca gatcggcaag gtccacgggg
    23161 acccgctgca gctcgatcgc gcccgtgtcg cttcgggcga gcatcagcag gtcgtcgacc
    23221 agcccggtga gctgccgcac ctcggcctcc acgtcgtcga gggcatcggc catggagctg
    23281 acgggccggt ccgcgtgccg acgcaggcgc tcgacgcttc cgcgaatgac ggcgaggggg
    23341 ttgcggagct cgtggctcgc atcggcggcg aactcgcgct gacggcggag cgactcccgg
    23401 atcgggacga gggcacggcc ggcgtacagg aagccgaacc cggccgccgc gaggagcgcg
    23461 gccagaccgc cgatcgcgag cacggcgagg accaccgaca gggtgcgcgc ctccgtcgat
    23521 cggtcctgga gcacctggag gacgtacgtc tcgccgccga cgtcgaccgc ctcgctgaga
    23581 acccgaacgg gcgtgtcgtg ctcgtctccg gcaccgatcg agatcgtcgc cgtccggagg
    23641 tcacgctggc cgtccagcgc ggccgcgaag ccggccccca ccggcaggtc gggcgagatc
    23701 gtcgtcgcct ccgggagcac ctgccggccc gcggcgtcga agaggaggag ggcggtgccg
    23761 gacgccgggc cgccgaacag gaactggccg aactgctcgt catcctcgtc gggctcgggc
    23821 cgtgcctgtc cgaggagaat gcgtcgggcg tcggcgaccc tcgcctcgag ctgcgtggtg
    23881 gccgatgccg ccagcgacgc ctgcgtgctg aagtacagcg cagatccgag caccaccagg
    23941 agcacgagcg tggaccccgc gctgaacagg acgagccgcc accggacccc ggacagcagc
    24001 cgagcgtcgc cggcgtggat cgccggaccg cggttgccgc tcaccccggc gccatgcggt
    24061 agccgacgcc acgcacggtg ccgatgcgct tcgcggcatc cgcaccgagc ttgctgcgca
    24121 ggtagtggac gtaggtatcg accgtgttgt gggtcaggaa ctcgccgtat ggccaggcgg
    24181 catccagcag ctggtcgcgg gtcatcacct gccccggatg gcgcagcagg cactccagca
    24241 gggagaactc gcgcgggctg agctcgatcg gccgtccgtc gacgctgacc agccgccgtg
    24301 cctcgtcgag ccggatcggg ccattgcgca gcaccggctc atccgtcgcg cgctccaccg
    24361 atccgcgccg cgccagtgcc cgaaggcgcg ccgcaagctc ctggtaggcg aagggcttga
    24421 ccaggtagtc gtcggcgccg gcgtccaggc ccgcgacacg atcctcgatc gcatcgcgcg
    24481 ccgtgaggat cagcatcggc gtccgcacgc ccgcacgacg cacgcgctgc gccacctcga
    24541 tgcccgagat gtcgggcagg cccaggtcca ggatcacggc gtccagctcg cggttggcct
    24601 cgatggtctc ggccgcggtc tcgccggaat cggtcagctc cacaaggtgc cggtcatcct
    24661 cgagcagccg ttggagcagg cggccgagtc gcgggtcgtc ctcgacgacc aggaggtgca
    24721 tcggtcagtc catccctcga gcgctccgac ggtggtcgct cacggtacaa ccgtgcgcct
    24781 cgatcctggg agtgccatgg gaattggctg ggaggccggg ggctgacgcc ccggcgtgac
    24841 ccagcgctac gcggtctcgg ccgcgagctc cacgaagtcc atggccgaca ggacaccgac
    24901 cgggcgtccc gcatcgtcga cggcgacgac gcgatggaca cggtggtcga ccatcaggcg
    24961 ggccgcatcc tggaggctca cctcgcgatc caccgtgatg gccggacgcg tcatgacgtc
    25021 acccacggag tagcgacgca gggcatcggg gttgatgtcc ttgaggcgca gcgcatcggt
    25081 caggctgtag acgccaacga tcgtgccgtg acggttgatg acggggacgc cactgatgcc
    25141 gtagtcacgc agcacggcgt cgacgttggc gatcgactcc gactcgtaca ccacgatggg
    25201 ctcgagcgac atcagatcgg cgacgaccag gggtgggacg ggtcgcatcg gggtcagcgc
    25261 gtgcgtcgtg ctcatgggcc cagcatcacg accgcatccg cggaccgaga gggccatgac
    25321 gccctgccgg ccgggacatt gggcatcggt tcgcctgccg gtgcgccaat tccgcgagca
    25381 ccggggcgga cggacggcgg tacccccgct gccgaacgtc ccgggcccca gatgacctcc
    25441 ggccctgaga caccgggccc tccagaggcg agactcgtcg agttgtgacc ctccacctct
    25501 tccatgacct ggcgatgatc gcgctcctcg gtttcgggat catcgcgtcc atcctcgcgc
    25561 cgcgcgtctt cagccggcgc tgaacacggc catccgcggc atcggccgcc ggagctcgcg
    25621 acgcccgcat ccgatcgatc ggcgcctcgc ggcccggcgg ctcgtgctgc catccgcgca
    25681 cggctaggat gcgtgcggtt cgcgggagcg tccgagggcg tcggaaggga ggggcatgat
    25741 cgggatcggt ctgctggcgc tcgccgccgt cctgctgttc gccggagccg agctcttcgc
    25801 cgataacgcc gccggcgcgg gccgccggct cggcatcacc gcgctcgcgg tggccctgct
    25861 gctggcgggt gccgagcccg aggagatgat caccgccgtc ctggcggcca tcagcggacg
    25921 acccgatctc gcggccggcg acgccgtcgg cgcgaacgtc accatgctca ccctggtcct
    25981 gggcctcacg gccgtgctgc gcccggttcc gttcgggaat cgagtccgcg agtacgcggc
    26041 cggtgcggcc atcgccggcg tcgcggccgt cgtcgtcctg ctcgacggtg gcgtgagccg
    26101 cctcgagggc ctggcgctca tcacggtcta ctccgccctg gtcgccctcg tctggtggcg
    26161 cgagcgagag ccgccgctga tcggggaagc ggcggaggtc gtcgaggatg ccgacgcacc
    26221 agcgccgcag ggaacggcgt ggatgccgct cgccctcgcg gtcgctggga tcgggatcat
    26281 gctgggcggt ggctgggtcg ccgtcgaggg cgccacgcgc ctcgtggggg acctgggcat
    26341 ccgcgacagc gtgatcggcc tgacgctgct ggccctggcc acgaccgcgg agctgttcgc
    26401 gctcgcctgg tcggcggcgc ggcgcgagct cagcgagctg gccgtggccg ccgtgatcgg
    26461 ttcggcgggc tataacgcca ccgtctccct cgggtccgcg gccgtcgcgc agccgctcgc
    26521 ggtgtcggga gtcctcggtg cggccattgc cggcgccgcg cttccgatcc tcctcatcgg
    26581 gctcggctgg tccggccggg tgggccgcgc gggcggcgcg acgctgctcg ccgggtacgc
    26641 cgtctacctc ttcgtggtgc tcaccgggtc cggcggcgtc acgccgtcct gaggcccgat
    26701 cgcgcgttca gcgggtccgc gccgccaacc tgcgcgtcga gttatcgaac caggctgagg
    26761 cgaatccgat acacaccggc cgcggggatg cgcccatggc cgctcgttcc ccgcagccga
    26821 tgcaggggta gcgctcgtcg agggtgtgga gcgatgcgcc gtggccgcat cggtcacagg
    26881 tcggctcgtg gtcgatccga taggcgcggc gccgatggtc cagctgcgat gacgacaggc
    26941 tcatgccgca tccgggccgc atcgggcgtg ccgggacggg tgggacatgg ccagtacgtt
    27001 cgggcgacgc atccctgcca aggtgagtgt cgagtggggg ccgaatgacc ctagggacct
    27061 cgacctccgg gaggggaacg tatccacgag gcgtggggac cggtcgcggg cgggatgacc
    27121 gcgcaccgcg gccgagtcgc cgagtttcgt cactggcagc cacgtgggtt cgccgcgctg
    27181 tcgctcctgc tcgtgccggc gccgtagagg aggaacgaca tgtccaaggc aaccgtcacc
    27241 cgaatcttcg tcggcagcgt gatcgccgtc gtcgccggga tcatcctcgc catcgtcgcc
    27301 ctgctggtgg cctatgccaa tggcgccttc gtgatggatg ggccggacgt cgttggcatt
    27361 cgtggcacga ccttcgcctg gaccatggtc gggctggccg tcgttgccgg gctggtggtg
    27421 atggccggct tcgtcggcgg catggtcgcc tgggtcggcg cgctgctcaa caccgcgcag
    27481 ctgaccgaca agacctggtt catcatcctg ctgctgctcg gaatctggaa cctgggcgta
    27541 ctggcgatga tcgcctacct gatcgcgggc cccgatggca cggccgccgc gccggacagc
    27601 gatgcggctc tgcgacgcga gacgagccac gcctgatcgg ggcagcgcga gcgcgggctg
    27661 gtcacggtcc cggccgacga cgaagccgcc gcccgcggtg ctgctccacg ccgactcgag
    27721 gtgatgctga tgaccgccga atcgtccgcc cagatcgcta caccgaggag acacaccccg
    27781 atggagaccg ccttcaacat cttcgtgttc ctgctcttcg cggccctgtg ggtcggcttc
    27841 gcctacgcgt tgattgccag ccagggatcg ctgaacgcgg cgtgggactg gatcaccagg
    27901 cagcacgtgg tcgtccaggg cgtggtgtgg ctgctgttcc tgcccgtcgt cgccggcctc
    27961 tggatctggc acaccgactg ggcgctcgtc gtgcgcctgg tgctcgtggt cggcatcggc
    28021 ttctggaaca tctacctgtt cttcccgagc tacctcctgc gacgatgacg tcgtcccggg
    28081 cggggaaggc cggcccgccg gcgccgccgc gcatgaagct ggggtggcct acggggcttg
    28141 aacccgtaac cttcggagcc acaatccgaa gctccgaccg ccgattcgtg ctcagatgtc
    28201 acgctttcgc ggccgaatgt tcgcatccgt gttcgctttc ctagtcggct cggtcacgga
    28261 caggtgcacc gagcaggtcc cggcgctcgt gcggagcggt cgcctcagtc gaggggcgcg
    28321 cagacccttg catcacgccg atcatgagcc aggcgcagga agtcctcgta cgaaggtgcc
    28381 cgacgatcag cggtcgtcgt tgacgtcggg agtcgactcg tcgaacgtgg ggttcgtgaa
    28441 ccgcgatctg ctcgtcacct gcgccgccct cgcctgcctt ctcgcagcgt gcagcagcat
    28501 tcgggaggta tggccaaccg accccggcga tgtgggctgt ccgaatagcg tcgaggcggc
    28561 cagggccgtc acactgggcg acaacgcatt cgtcgccacg ggctacgtga ttcgcttcgt
    28621 gccctccccc gacgaggcgt tccgcggcta cgacctggac gtgacccgcg tgttcaccgg
    28681 ccccgcgttc cgcgatgtcg ggttcctgcg gatcgaggac gagatcgccg gcatgtcgca
    28741 gggcgacccg gtgctcgtgg ttgccgagcg gacggacaag gcccgggtct tcgttcccgg
    28801 accgtgccca ccgctcgtac gggtccccgc cgaggacgtg gagccgtgat cggccgtgtc
    28861 gccgtcgccg gtgtgctgct gctgggcgga tgcggcagcg tcgctccgag gccatcggtt
    28921 ggcgaggcca tcttcttccc gacgcacgaa cacacgcccc agaccgggct cccagcgggc
    28981 gccatcgccg gcaccctggc gctcgaggat ggatgcctgt gggtcgtgac cgacgacaac
    29041 acgccgctcc tggccctgtg gccgagcgac tttcgccttg agacggagca aggccgcctg
    29101 atcgtcatcg gccgcggcgg gtcggtaccg ctcggcggac ggatccacgc tagcggcggc
    29161 gagtacgcca ccgatcaggt ggccttcgtc gagcagcaga tcagcgccga gatccccgat
    29221 gcatgccgcg gccggggctt ctggctggtc ggcgagctga tggactagga gtcctgctcg
    29281 tcactcgtcg atgtccaggc gaggatcacg gctcgtcgcg ctaaggcgcg ctcgctcgcc
    29341 gcccggcgcg aacgccgcct ggtccacgca tccagtgggg caggacgacc atcagctcgg
    29401 cgttgcccgg gaggccctcg accgagcgga agaggatccc gctcgagtgc tgaccgtcgg
    29461 gcgattggaa cggctccgcg agcgactcgc gaccgttggg cacgtcgagc gcgaccgagc
    29521 agaagatacc gtcgtcgcag tacaccgctt cgagcacggt gaactcgaac tgcgaccagt
    29581 cggcgtctgc ggccgcagcg acgtactcct cctggctgcc ccactcgtcg cgggccgact
    29641 ggtggagcag ggaccagccg cgaccggcct cggcgcccga cagtgcgcgc aggtaacgct
    29701 ccgccaggtc gaccgcctcg ctgcgccggc ccgggttgtc cggaggagcg cagccagcca
    29761 ggagcaggat cgcgacaaca acgaggccga gcttgctcgt gaaccagctc cccgccatcc
    29821 gcgtgggatc agcacccgtc cagcgcggct tcggccgcgc cgagttgggt ccgcgccagg
    29881 tcaagctctt cggcggtcat gccgtgggtg ttcgcccact cgggtaggag cgctttcgcg
    29941 gcctggccga cgtggaggta gcccatcacc agcgcctggt aggggtcact ggcgactgtc
    30001 gcgtccccat ccacggcatc cagccgtgcc ttcgcctcgt cagccagccc cgatgcgtcg
    30061 gaggccagct gccttgcgcg ctcggtgtct ccggcccggt cggccccgag cgcgttctcg
    30121 gccacgccat aggcgtgttc gagcccaccg gctacctcgc acatcgccgc gctccggtcc
    30181 tgcgtgagcc cacacccgac cagcgccagc gccagaggca ggaaagcaag gcgtcgcatg
    30241 gcctacgcgc gctggccggg cggcggcgcc agcagcagga gggcggccgc gaggcacaac
    30301 aggccgccga ccagcggcag gtaggtaggc gtcggcgtcg gggcgtacag gccgacggtc
    30361 cacagcgcaa accaggtctg cggggctccg gccccgacga ggatgccgcc gcttgtccag
    30421 cgatcaagga cccccacggc ccgcaacgcg gagacagcga agcacagcaa cgggacaccg
    30481 agcccggtca tcaggatcgg cagctccacg gacgaggcga acggcccgtc ggcgaacagc
    30541 tcgagcggtc ggatagccag cacgacaccc gccgcgattc cgagcacaac cggcggccac
    30601 ctgctcgcca gcagcttgcg aagcatgctc gtcctgccgt acgcgatacg gtgttccatc
    30661 tggctccctc cttccgcctg ggtgcggcgg ctctccgagc atcgcgtcaa cagggtgcct
    30721 tggcaagccc ttggggagcc gggggtgaac gacgtggact gaacgtcagt cagccggcag
    30781 cgccaccgac tccgcaagcg ccacgaactc gtcctggctg aagtcgctga ggttcccgac
    30841 cagtgccagc tcgtctgacc cgagctgcca gacgagcacc atctcgccgc tcggctggtg
    30901 cagccacaag gtcgccggct gaccgttgac ctccaccggt ggctggcggt tgccgccggc
    30961 ggtgttcact gggccgccga ggccctggat gagcgtgagc atgctgtcgc cctcggccgg
    31021 cagcggatcg gactccggtg accacacccg ctcccatcgc ggcgtggaac tgccagcgac
    31081 gttgccctca cgaagcagct cccatccgtc gggcaacgca ccgatctgcg cgagtcgggc
    31141 tggcggggcg agcaggaaca cttggccagc caggtcgcgg acggcggccc cggtgaacgg
    31201 ctcgtcgagg tcaagcgcga gcgatctggg gtagcccacg ccggcgcatc cggtgttcgg
    31261 cgggagcggc ttggggtgcg gctcggcgta gatgccgatc tccaactcgt cacccacgat
    31321 ttcggcggtg ccgtggtagg ccaccgagca cgggttgtcc ggatcgaact cctcaccgcc
    31381 gatgaaatcc acccgcaccg agcgccggtc gttggccagg tcgacgctgt cgaagcggac
    31441 cgggtcgctc gggaccggcg ccggctcagc ctcgagcagc tcgctcgtca ctgcgcaggc
    31501 ggagagcacg gctccgagga caagcatcac cgacgccttc agcatgcgca ccgttgctcg
    31561 ctcggcgcca acgggatgga cggcaggcga ggtgctcgct gcctcggtcg tcatcgagat
    31621 cagcgcgacg tagcgagaag tcgccgcagg atgaccgtga tgccgtagac ggccgccaac
    31681 gcaagtgcag cgaaggtcag cggcgagtca agccgagaca tccccatggc gttgccgatg
    31741 acctttccgg cggtgagcgc gacgaggctg aggacgatga gtccgccaac gagtccagcc
    31801 cacgaccagg cgtgccgcga acgcggggtg gtcgctgaac gacgtccgag gtcggtgctg
    31861 gccagaagcg cgatcgcgac gggaacgagg atctcttgca gcgcgcgatc ggtatcgaag
    31921 acgaggccgg atcgctggtc ctcgaagatc acgtaggccg agccggcagc cagggtgaag
    31981 gcgaggacca caagcacgcc gggcgatacc aggcgcccct cgccgaggag ccggccgatc
    32041 cacgggatcg ccgctagtag tgacagggag agcaggatcg cgttgagcag ggcgaagtgc
    32101 tcccgttcgg gttccgcgat gccaagctgg ctgctgatcc aggcggccgc gaacgtggtg
    32161 atcagcagca ggagcacgaa caccaccccg gtcaacgcga tccagaccag ggcgcgcggt
    32221 gcctcccgag tcacgacgac ggcttccatg cctcctctcc ctcagagctt catcgcgacg
    32281 ctgatggcgc agcgtcggtg gccgctcgat gaccgctagc caccggaccg cgtcgactgc
    32341 gccgacgaac gctgccaccc cggatgacgg caagtgcgac gctcgcgctg agcgagatcg
    32401 cgccaagcgt tccgagcagc gacccggaac cctgctcggc gggcatggac gtgtcaggca
    32461 gcagccgcag gtagccgaat agctcatcgt gcgtggcggc tggtggctca ggcgccagcg
    32521 tgccgaccgt gccgtccggc agcaggtacc acaccgccga gctgaagagt ccgatgtcgg
    32581 tcgggtcctg gagcgccacc accacgcgat cgccgatctt gagctggtgc gagcagatcg
    32641 gagctgtcga cggcgccggg tctccgatga cgatgctctc ggcctgcggc ccacggaaga
    32701 cccgttcgac gtccagctcg taggtccact cgctcggctg ggacaccacg cggccggcca
    32761 ggatgatcgt cgcggcggca accgcctcct cgcgcgtcgg catcggtccc gagcacgcca
    32821 acgccgcctg cggcgtgacg aaggcggccg ctgccagcgc ggcaaccgcg gtcgccagcc
    32881 gcatcgagtt cctcatgtcc cctctcctcc attcgtggtg ctctgccgac gtaatcgggc
    32941 aagcgagcag gcgctcaccg accaggagca tcggtaggag tgacgcgcag gcaaccccga
    33001 tcgtgacatg gtcggcaagg cggctgtgcg ccgcgccgcg gctcgcgaag tcatccgtcg
    33061 ggcggaggcg agtcgaagtg ccagcttgag atcacggtct gaagctgtgc gggcgcggtc
    33121 tgagcggctg ccgggcccca gcgcgcctcg atgaactccg gcgcaccggg aaagtgccac
    33181 cgcatcgacg tcgacgttcg ggacaggatc gcgtcgcgtc cgccgacctc gacccactcc
    33241 tcaccggcag ccaaggacgg ggtggcctgt ggcatcggca ggtgtgcgct gaagtggtag
    33301 gcgaccacga tcccgtcgtc cgggacggtc cactctggtg ccgacccgca ggagaccgat
    33361 gtgctggtct cggtacaccc gaggtcgaag tcaccgatgc ccacggcggc gagcaccgtc
    33421 ggcccatggt gaccgtaatg tcgcgggcca gcgatgacct gccagtcgtc cgggtagtcg
    33481 aagccaaagc catccccgcc gaagtgcgtg tagtgaacgc cgccgcccga cgagtagggc
    33541 gggttcagcg agagcatgag ggcgacgatc ccgaacagga ggccgatcgc cagcgccacg
    33601 agcaccggtc gcagcggccg ccacaccgag cctcgcctgt ctatcgcagc tgagcccacg
    33661 attgctctcc gtatcgcgcg tctctcctaa cccgccgccc gatgacgcta gacctgacac
    33721 agtcgacagg caagcgagat cccagcccac atctccggta ctcgcgtgga ggccaatgcc
    33781 gacccaactc ccgacgcgtg accaccttcg tcaggccagt tggtctacgg gtcgtcctca
    33841 agtgccagac gcccttggtc gtcgatgtgg acgtaggcgc cgctgtcttg ggccaccggt
    33901 gcgagcagat catcggagta caggaagccg cggtccccgt tcgagaatcg aagggtccac
    33961 ctgtcgatcg agacacggat gcgaaccgcc tcctcttgac caggatcgac gctgatcagg
    34021 ggcgcgacga ccgccagcac gccgccgggc acgtcacccg gcggttcggt gatctccaga
    34081 atcgccgcct tgtcccaatc gttccggatc gtgatggtca cgtcgcgcgc cgtcggcgac
    34141 accaggtcgc accctacaag gagaacggtt gcgcaaccca ccgctagccg gcgactcatc
    34201 gacctgacaa gcgggggcag gcacgcggag ggcatgtcga gtcccgactc cggcacagag
    34261 cgccagacga caaacgtcgg gcgccgccta gcgtctgcgc aacgcgaggt gaacagccac
    34321 gaacacgacg gacccta
//
LOCUS       DASTYP010000158        15612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_339952, whole genome shotgun sequence.
ACCESSION   DASTYP010000158 DASTYP010000000
VERSION     DASTYP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15612
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339952"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VFV59_07260"
     CDS             complement(<1..990)
                     /locus_tag="VFV59_07260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEU5325664.1"
                     /translation="MDRIVVLGGGVGGTLAANLLARKLRRRIDAGSVNVTLVDEGGEH
                     VYQPGFMYIAMGGEKPESLRRSERSLLDRRIQLRIARATRIDPVARCVEVADGEPIGY
                     DWLLIATGARIVPEEIPHFAEEAHHFYGVDAAQRLRAALDAFDGGKIVVAIAGMPYKC
                     PPAPLEVTFLIDSELRDRKLRDRSEVHFCSPIARAFTIESVSEMATPILAHKSIGLHT
                     FFNVEAIDPERKVIQSLEGEELPYDLLILVPPHRGAQVVIDSGLAPPSGWLPTDPHTL
                     EVKGQERMYAVGDATDLPLSKAGSTAHFEAPIVVERIVAGVEGREPDAVAGNYT"
     gene            complement(1000..1236)
                     /locus_tag="VFV59_07265"
     CDS             complement(1000..1236)
                     /locus_tag="VFV59_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010901577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU5325665.1"
                     /translation="MEVPINRTIDARGSFCPGPLMELIRAIREGQVGEVIAVWSTDSG
                     SRVDIPAWIEKAGHRLVGVESRDGYDEIIVEKLR"
     gene            complement(1263..1934)
                     /locus_tag="VFV59_07270"
     CDS             complement(1263..1934)
                     /locus_tag="VFV59_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEU5325666.1"
                     /translation="MTVATPSRVRNPSLFDVLVGAVADEPQFVAEARATEAPPTAVLD
                     APIEQEAVPATEPVETEPKPDAKKNELLIIFESGDLERTWATLILATSAAASGMKVSI
                     FLTFWGFYPLVKPGASPGETWMQKMLAVMNRPGIDHLKLSRLNFMGMGPWMMKKLAKR
                     YRVAEPRELLEIAMAMGVHLIPCQMTMDMFGLTRDDLIDGLEEPAGAATVLGHVADGV
                     PTLFI"
     gene            complement(1945..2178)
                     /locus_tag="VFV59_07275"
     CDS             complement(1945..2178)
                     /locus_tag="VFV59_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU5325667.1"
                     /translation="MTLEIANRLDARGLACPMPIVKTAQAIAGMTSGQLLEVLATDPG
                     SVKDFAAWSKTTGHEIVEQGEDAGVFRFVLRKK"
     gene            complement(2194..2607)
                     /locus_tag="VFV59_07280"
     CDS             complement(2194..2607)
                     /locus_tag="VFV59_07280"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEU5325668.1"
                     /translation="MSDRLAITLFSGTEDRLNAAAVLIAGAAALGRPVHVFLQYWALD
                     AFRTDRIDAGRGLAPEAGPEGEAAVRSLRQRGQASWVETLRQAKELGGVEIQACSLSM
                     DLLAIEQSDLDPLVDGVEGVTAFWLAADDAQLLFI"
     gene            2847..3752
                     /locus_tag="VFV59_07285"
     CDS             2847..3752
                     /locus_tag="VFV59_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5325669.1"
                     /translation="MRIVLGVDGSTAARVACDFVAGRSWPTGTRIRIVAALEPRVDWT
                     GLVPGGNGGSEEERRLLEEVLDVRADCLRERAYAVEPVLEIGRPAELLIAHADQWFAD
                     LIVVGSRGLGAAASALLGSVSAHLVDHAPCPVLVARSPEATRMVLAADGTESSMGIPR
                     ILGSWGAAFRGLPVEVLSVSAHEERPTPWGPAGEGSEEETTDLELHRGIAEQVADEMI
                     ELGWSAAALVRAGDPGREIVATGREWGADLIVTGSRGLGTLRRFVLGSVAHDVLLHAR
                     SSVLVMRGHVVAPISEAARAAIPSV"
     gene            4173..5525
                     /locus_tag="VFV59_07290"
     CDS             4173..5525
                     /locus_tag="VFV59_07290"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5325670.1"
                     /translation="MQRSLLYFAAFTGMGTVFVATLLVAGLVLGATNRPDQGEAQPSL
                     AAPTASAPVAEGPVGEITITAFDLGFEPAMIHVDEPGTYAVNFVNDGGIYHDVTFADG
                     TTIGAEGHQTGTGEVVVPDGGITFICSVPGHSDAGMTGEVMVATASESGAPEPSPSGT
                     LTAEQMRDIDAAVTAKFPAETEGKGNQILEPEIQPDGTLQWELTASVIQWETEPGKVV
                     EAYAYNGMVPGPQLRAEVGDKIRIILHNELPEPTTIHSHGLFVPPEMDGVPVISQPAV
                     MPGESFTYEYTLRNAGSHMYHSHFMAEHQVPMGLLGAFVVTDPNDTEVPAVDQDIAMI
                     LNDGPLGFTINGKGFPATEPIVARQGEMIRIRYMNEGLQIHPMHLHGIPQLVIAKDGW
                     TLPQPHYEDTVLVAPGERIDVLVEATELGTWAFHCHILTHAEGPNGMFGMVTAMVVTE
                     "
     gene            5540..6037
                     /locus_tag="VFV59_07295"
     CDS             5540..6037
                     /locus_tag="VFV59_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325671.1"
                     /translation="MAEHSSDGLVRLAASATLRAHPATVARLLGEEAGAWPGEPLPDA
                     PHGMRRFAVDLRLRVGGEGSGLATFRKAAFLDLGEPMRTPRGWRVEIGWQASTAAPLF
                     PVFSGALAVEDGVMRLEGLYAPPGGVLGRVADRLLLHVAANGTARWLLDELDRVALER
                     EASAG"
     gene            complement(6047..6724)
                     /locus_tag="VFV59_07300"
     CDS             complement(6047..6724)
                     /locus_tag="VFV59_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5325672.1"
                     /translation="MTDESRERPLRLLVVDDHEVVRQGIVALLDRREGFEVVAQAGTV
                     AESISMAARFEPDVIVMDVRLPDGSGIEACREIRSTRPETRVVMLTSYPDEEAVLSAI
                     IAGASGYLLKQIRGRDLVAAIESVGRGDSLLDPAVTEKVLQRVRTAASGGASDELAEL
                     TSQERRILLLVAEGKTNKEIASEVFLSDKTVKNYVSSILSKLNLQRRTQAAAFVAKHH
                     LGEPPGV"
     gene            complement(6721..8247)
                     /locus_tag="VFV59_07305"
     CDS             complement(6721..8247)
                     /locus_tag="VFV59_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5325673.1"
                     /translation="MTSTPEASRRTLLLPPMLRAIEAWLDEPSATRADALGAAIAEVC
                     EPLGLAVGRVDLVAPPLPELHLDLGARDAVATPIRMPDADAGTVMGSVELAGDPEQAA
                     AFRSALEFVFLAVQGRASASRATRQLAALDVAVRGIGAQRELERVLQEIVDRVRDLAD
                     AEYAALGIVDEDGAIIRFITSGISHEHRLRIGELPRGRGLLGLIIRDNRTYRIPDIAT
                     HPERYGFPPHHPEMHAFLGMPILARGEPVGRLYLTNKRGAREFTADDERLVEMFALHA
                     GIAIQNARLQDHERRLAIVDERDRISRDLHDSVIQSIYAQTLALDDVPDLVEDDPEEA
                     ARRVDDAIEALHGVIRDIRNFIFGLRPVLLESGSLEEGLRHLATELRRSGAVDVEVRV
                     EDPAGGLDELPIELVAELLAVAREALSNIARHAAASHASLAVQSAVEELRLEIVDDGR
                     GIDPEAATARGHHGLPNMRARIEALGGTFEVARATEGGTRIIVAIPRRAAGTRETERP
                     "
     gene            complement(8261..8662)
                     /locus_tag="VFV59_07310"
     CDS             complement(8261..8662)
                     /locus_tag="VFV59_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5325674.1"
                     /translation="MRIELTRRGDYAVRAMLTLARPGAGQLTGAALADATAIPATYVP
                     QVMGDLVRAGLVENRRGRHGGYLLAREASDISLLAVVEAVEGNGRRRRCVLRGGPCRR
                     DGECDVHAAFFAAQEAVFDRLRAVSLADVAR"
     gene            8787..9920
                     /locus_tag="VFV59_07315"
     CDS             8787..9920
                     /locus_tag="VFV59_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5325675.1"
                     /translation="MQRYFFYGGLAGGGSALFVSLVLAGSVVVNGLMPGSATGANDPA
                     TAGGGMNHDMSGMNGGGATAGVNGTLTEADPNAPAYTPRDATAPDLMEGEVHDIDLYV
                     VEQQMTVADGYAQTVYAFGFTPETATVPGPVIRVKVGDQVRIHFKNPATNAMPHSVDF
                     HASLVAWNDEMTDINPGEEKLYEFEAKYAGVYMYHCGTAGALSHMLNGMYGLIIVEPA
                     EGLDPVDQEYFLIQNEWYIGQQGGTGDLERANAPVPSPDFVVWNGIANQYKDNPIQVP
                     VGEQIRVFVLNAGPNIDTSFHVVGTIFNRVIKEGVELARGNEGNWGSQAVDLAPAQGA
                     IVEFNFDEAGLYPIVTHAFNFVAHGALGLFQAGDGGAGAGSGH"
     gene            9983..11224
                     /locus_tag="VFV59_07320"
     CDS             9983..11224
                     /locus_tag="VFV59_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325676.1"
                     /translation="MTERHILALGLALSGTFLVAAVSAAFVTVVAGGSSWAALHLALA
                     GAAGVAIGAFMPHFAITLAGTAPQPAPQRLATIGLLATGSALAVAGVALLGGRVALGG
                     SILVVGGLALVGWQTVAPLRSPLARRHPIVTVTYLLALAELATGVLLGGLGAAGWDPV
                     TRAWAALRPAHAWLTLLGAVSLTIFGTLIYLAPTILGARIRASRTLAVAATGMLVGPP
                     LAALGFALLATPVVLAGTVLTLVGALGQISYVVDTLRRRGRYTSEHDWRRVAVWHLAA
                     GTGWFAAAVAAALVGFVDGGRIAAWSLGALAVPLVAGWMAQELVGSWTHLAPSVTPGS
                     PGRHAEQRRTLAVASRVRPIAWNVGVGLAWAGAALGVTPLAVGGGALLAASAIASVAL
                     LLRALARQGAGVRPGASAPAG"
     gene            complement(11193..11456)
                     /locus_tag="VFV59_07325"
     CDS             complement(11193..11456)
                     /locus_tag="VFV59_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325677.1"
                     /translation="MTRTPELDAPAAEPTFEVIRRFVVVRGVRRPLRLVRFAKGWVAS
                     VETESGPSVGADHSPYLAARRALEPLGVDLVETLTLLGPMPRA"
     gene            complement(11453..12742)
                     /locus_tag="VFV59_07330"
     CDS             complement(11453..12742)
                     /locus_tag="VFV59_07330"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017868950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5325678.1"
                     /translation="MTVTDPRVEAGGRAASRLSALDSALAQFDRAADHLQLAPETRAI
                     LRVPKREWTVNFPVTMDDGRVEVLTGYRVQHNVSRGPAKGGIRFHPAADLDEVRALAM
                     WMTWKCALVDVPFGGAKGGVTIDPRALSQGELQRVTRRFATELQGIIGPETDIPAPDV
                     GTNAQVMAWLMDTVSMHAGYTVPGVVTGKPVDLGGSEGRADATGMGVMFTAERALAEL
                     GVSLDGATVAVQGFGNVGEAAARLIHERGGRLVAITDVGGGVANPDGLDPGRLLAHLK
                     ETGSIAGAPGTQPIDNPALLALDVDLLVLAALEGQVTGANAHTVRARVLAEGANGPVT
                     PDADPILAERGVVTVPDILCNAGGVIVSYFEWVQNLQSFAWPADEVAERLRLVIGRAY
                     DQVAEMRDAQGVDARLAAHAIAVGRVAEAQQIRGRYP"
     gene            12870..13847
                     /locus_tag="VFV59_07335"
     CDS             12870..13847
                     /locus_tag="VFV59_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5325679.1"
                     /translation="MSAAPLDDLLAVPVAALLDAPRRPDEVADLLGADAITVAPDRLE
                     RLLERMTALGLARVAGYDGEGRARYVVTSLGQRAAAALLTAEPELRVGLEELERLRTD
                     LLATVGHDLRTPLTAIRTSVGLLLDPALEPSPAQRQQLLTTIGRSADRMQRLLTQLLD
                     LARLRAGRVEMDRTRFDARELVHEVAAAIEPMASARSQRLTLELPPESVQVVGDRRWL
                     EQALLNIAANAQKFSPSGEEVRVGVTADGGTVRWSVTDRGPGIDAAAQARLFERFFVG
                     AADRGGGVGIGLPTALGVAHAHGGTIEVDSEIGRGSTFTLIVPRADDDA"
     gene            13844..14536
                     /locus_tag="VFV59_07340"
     CDS             13844..14536
                     /locus_tag="VFV59_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5325680.1"
                     /translation="MKLLLVDDDAEIRASVRLGFELQWRDVEIVEAAAGGEALAAVER
                     ERPDLVLLDVGLPDFDGYSVLREIRAFSAVPVIMLTARDQPIDTVRGLEAGADDYVTK
                     PFDHLELMARVRAVLRRLDLRAPASRTAAYRNGALEVDVEAREARVDGERVALTASEW
                     RVLELLVANAGWVVPYERLTARLGGRDEPGDLEGLRVFVRRLRLKLGDDAHRPRYIET
                     VRGLGYRLLAVA"
     gene            14610..15365
                     /locus_tag="VFV59_07345"
     CDS             14610..15365
                     /locus_tag="VFV59_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5325681.1"
                     /translation="MLRRSVTWIALLAAVILVWLQLPEDWPISAPGDPLNGYLTWAFW
                     ILSILVVLILFTYRRDPAATEVEVEGPAFARFLFANSRAGLFWLPVRLFLGFSWLTAS
                     LHKIADGGWLDGGASLAGYWQNAVSIPEGGRPPITYDWYRDFIQLLLNNGAESWFSWL
                     IVFGELLVGIGLIVGALTGIAAFFGALMNMSFLLAGSASSNPVLFTAAIGLMLAWRVA
                     GYYGVDRWLLPMLGTPWRAPVAQRAATGAPNPG"
BASE COUNT         2100 a         5607 c         5622 g         2283 t
ORIGIN      
        1 ggtgtagttg ccggcgacgg catcgggctc gcgaccctcc accccggcga cgatgcgctc
       61 gacgacgatg ggcgcctcga agtgggcggt ggacccggcc ttcgacagcg gcaggtcggt
      121 cgcgtcaccg accgcgtaca tccgctcctg gcccttcacc tcgagcgtgt gcgggtccgt
      181 cggcagccat ccggacggcg gcgccagccc cgagtcgatg accacctgcg cgccgcggtg
      241 cggtggcacc aggatgagga ggtcgtacgg cagctcctcc ccctcgaggc tctggatcac
      301 cttgcgctcg ggatcgatgg cctcgacgtt gaagaaggtg tgcagcccga tgctcttgtg
      361 cgccaggatc ggcgtcgcca tctccgagac gctctcgatg gtgaacgccc gcgcgatggg
      421 ggagcagaag tgcacctccg accgatcccg cagcttgcgg tcgcgcagct ccgagtcgat
      481 gaggaaggtg acctccagcg gagccggcgg gcacttgtac ggcatcccgg cgatggcgac
      541 cacgatcttc ccgccgtcga atgcgtcgag cgcggcgcgc agccgctggg cggcgtcgac
      601 gccgtagaag tggtgtgcct cctccgcgaa gtgcgggatc tcctccggca cgatccgcgc
      661 gccggtggcg atgagcagcc agtcgtagcc gatcggctca ccgtccgcca cctccacgca
      721 gcgcgccacg gggtcgatcc gggtcgcgcg cgcgatgcgc agctggatcc gccgatcgag
      781 cagcgaccgc tcggagcgcc gcaggctctc cggcttctcg ccgcccatgg cgatgtacat
      841 gaatcccggc tggtagacgt gctcgccgcc ctcgtcgacc agggtgacgt tcacgctgcc
      901 cgcgtcgatg cggcgccgga gcttgcgggc gagcaggttg gcggcgagcg ttcccccaac
      961 gccgccgccc agcacgacga tgcggtccat cgccgcggcc taccgaagct tctcgacgat
     1021 gatctcgtcg tagccgtcgc gggactcgac gcccaccagg cggtggccgg ccttctcgat
     1081 ccaggccggg atgtcgaccc gcgagcccga gtccgtcgac cacacggcga tcacctcgcc
     1141 gacctggccc tcacggatcg ctcgaatgag ctccatgagc ggcccggggc agaagctgcc
     1201 gcgcgcatcg atggtgcggt tgatggggac ctcgatggtc tgcatcggtc actcctctcg
     1261 tgtcagatga acagggtcgg gacgccgtcg gcgacgtggc cgaggaccgt ggcggcgccg
     1321 gccggctctt ccagcccgtc gatcaggtcg tcgcgggtga ggccgaacat gtccatcgtc
     1381 atctggcagg ggatgaggtg gacgcccatg gccatcgcga tctcgagcag ctcgcggggc
     1441 tccgccacgc ggtagcgctt ggccagcttc ttcatcatcc acgggcccat gcccatgaag
     1501 ttcagccggc tgagcttgag gtggtcgatg ccgggtcggt tcatgaccgc cagcatcttc
     1561 tgcatccagg tctcgcccgg gctggcgccc ggcttgacca gcgggtaaaa gccccagaag
     1621 gtcaggaaga tcgagacctt catgccgctc gcggcggcgc tggtggccag gatcagggtc
     1681 gcccaggtcc gctccaggtc accgctctcg aagatgatga gcagctcgtt cttcttcgcg
     1741 tccggcttcg gctcggtctc gaccggctcc gtggccggga cggcctcctg ctcgatcggc
     1801 gcatcaagca cggcggttgg gggagcctcg gtcgcgcgtg cctcggccac gaactgcggc
     1861 tcgtcggcga cggcgccgac gaggacgtcg aacagcgagg ggttgcggac gcgggacggc
     1921 gtcgcgacgg tcatcggtcg gccctcactt cttgcgcagg acgaaccgga acacgccggc
     1981 gtcctcgccc tgctccacga tctcgtgtcc ggtggtcttc gaccatgcgg cgaagtcctt
     2041 caccgacccc gggtcggtgg cgagcacctc gagcagctgg ccggaggtca tgcccgcgat
     2101 ggcctgtgcc gttttcacga tcggcatcgg acaggcgagg ccccgggcgt cgaggcgatt
     2161 ggcgatctcg agcgtcatcg tgcgcacctt tcgtcagatg aagagcagct gggcgtcgtc
     2221 ggcggccagc cagaaggcgg tcacgccctc gacgccgtcg accagcgggt cgaggtcgga
     2281 ctgctcgatc gcgagcaggt ccatcgacag cgagcaggcc tggatctcga cgccgccgag
     2341 ctccttggcc tggcgcaggg tctcgaccca gctggcctgg ccgcgctggc ggagcgagcg
     2401 gacggcggcc tcgccctcgg ggccggcctc gggcgccagg ccgcggcccg cgtcgatgcg
     2461 gtccgtgcgg aaggcgtcga gtgcccagta ctggaggaag acgtggacgg gacggccgag
     2521 ggcggcggcg ccggcgatca ggacggcggc ggcgttgagc cggtcctcgg tgccggagaa
     2581 gagcgtgatg gcgaggcggt cgctcatgcg gagtcgggac ctcggtagcg aactgttcga
     2641 cgttgtgtct caccctgcga cccgggcacg cgacgggcac gtgccagccg gccccttgca
     2701 tcggtccatc ccgcccgcgc gcccgcgcgt ggccgcgcgc tcgccatcgg gccgaaagtc
     2761 accccctcgc gggcctgacg ggactggccg cgccgcggtc atccgcgcag cgtcgagcgc
     2821 ggcagtcgcc gatggaggag attccgatgc gcatcgttct tggcgtcgac ggctcgaccg
     2881 ccgcgcgcgt cgcctgcgac ttcgtcgcgg gacgcagctg gccgaccggc acgcggatcc
     2941 gaatcgtcgc cgccctcgag ccgcgcgtcg actggaccgg cctcgtgccg ggtggaaacg
     3001 gcggcagcga ggaggagcgc cggctgctcg aggaggtgct ggacgtccgc gccgactgcc
     3061 tgcgtgaacg cgcctacgcc gtcgagccgg tgctcgagat cggccggccg gcggagctgc
     3121 tgatcgccca cgccgaccag tggttcgctg acctcatcgt ggtcggatca cgcgggctgg
     3181 gtgccgcggc cagcgcgctg ctggggtccg tgtcggcgca cctggtcgac catgccccct
     3241 gcccggtcct ggtggcgcgc tctcccgagg cgacgcggat ggtgctggcc gccgacggca
     3301 ccgagagctc gatggggatc ccgcgcatcc tgggctcgtg gggcgccgcg ttccgcgggc
     3361 tgccggtcga ggtgctcagc gtatccgccc acgaggagcg gccgacgccc tggggcccgg
     3421 caggcgaggg gtccgaggag gagacgacgg acctcgagct ccaccgcggc atcgccgagc
     3481 aggtcgcgga cgagatgatc gagctcggct ggtcggccgc ggcgctggtt cgggcggggg
     3541 atcccggccg cgagatcgtg gccaccggac gtgagtgggg tgccgacctg atcgtgaccg
     3601 gctcccgcgg cctgggcacc ctgcgtcggt tcgtcctcgg aagcgtcgcg cacgacgtgc
     3661 tgctgcacgc gcgctcgtcg gtgctggtca tgcgcggcca cgtcgtcgcg ccgatcagcg
     3721 aggccgcccg agcggccatc ccgagcgtct gacccgcgcc ccgagcgccg ggagtgcccg
     3781 gacacagatc gaccggcgga tatccgccgg tcgatctgcc acgggagggt gatccaattc
     3841 ggggaggagg tcccacgggt ttgacgttgc gccgtgccac ctcttccgtg tatcggtgag
     3901 cgctcgcgca tcccgacgcc ccaaaggcta cccgtccccg gcggccggca accctgccgc
     3961 ctggcccctt gctgacggga cctccgggcc ccgatggagc gcgcatgtcc cgatccgccc
     4021 gggccgttcg gcactcaacg ccgggcgatc ggtcgcgtgt aatgacgcca ccaacgcggg
     4081 ccccgggagc cgatgagcga cgcgcttccg gagcccgcgg cggcatatct gaacatcagt
     4141 accgaccttg aacgcacgac acgggagtat cgatgcagcg ttccctcctc tacttcgccg
     4201 ccttcaccgg catgggcacc gttttcgtgg cgaccctgct ggttgccggc ctcgtcctgg
     4261 gcgccaccaa ccggcccgac cagggtgagg cgcagccgtc gctggccgcg cccaccgcat
     4321 cggcccccgt cgcggagggg cccgtgggcg agatcaccat caccgccttc gatctgggct
     4381 tcgagccggc catgatccac gtggacgagc ccggcaccta cgcggtcaac ttcgtcaacg
     4441 acggcggcat ctaccacgac gtcacgttcg ccgatggcac gaccatcggg gccgagggcc
     4501 accagaccgg caccggcgag gtcgtcgtgc cggacggggg catcaccttc atctgctcgg
     4561 tccccggtca ctccgacgcg ggcatgaccg gcgaggtgat ggtcgcgacc gcgagcgagt
     4621 ccggcgcccc ggagccgtcg ccgtccggga ccttgacggc cgagcagatg cgcgacatcg
     4681 acgcggccgt gacggccaag ttcccggcgg agaccgaggg caagggcaac cagatccttg
     4741 agccggagat ccagccggac gggaccctgc agtgggagct gaccgcatcg gtcatccagt
     4801 gggagaccga gccgggcaag gtcgtggagg cgtacgccta caacggcatg gtgccggggc
     4861 cgcagctgcg tgccgaggtg ggcgacaaga tccggatcat cctgcacaac gagctgccgg
     4921 agccgaccac catccactcc cacggcctct tcgtcccgcc cgagatggac ggcgtgccgg
     4981 tcatcagcca gccggcggtc atgccgggcg agtcattcac ctacgagtac acgctgcgca
     5041 acgccggctc gcatatgtac cacagccact tcatggccga gcaccaggtg ccgatgggcc
     5101 tgctgggcgc cttcgtggtg accgatccca acgacacgga ggtgccggcg gtcgaccagg
     5161 acatcgccat gatcctgaac gacgggccgc tgggcttcac gatcaacggc aagggcttcc
     5221 cagcgaccga gcccatcgtc gccaggcagg gcgagatgat ccggatccgc tacatgaacg
     5281 aggggcttca gatccatccg atgcacctgc atggcatccc gcagctggtc atcgccaagg
     5341 acggctggac gctgccgcaa ccgcactacg aggacaccgt cctggtggcg cccggcgagc
     5401 ggatcgacgt cctggtcgag gccaccgagc tcgggacgtg ggccttccac tgccacatcc
     5461 tcacccacgc cgaaggtccg aacgggatgt tcggcatggt gaccgcgatg gtggtcaccg
     5521 aatgatggcg gcgcagccga tggccgagca ttcgagcgac gggctggtcc ggctcgctgc
     5581 ctcggccacg ctgcgcgctc atccggcgac ggtcgcccgt ctcctcggcg aggaggcggg
     5641 cgcgtggccc ggcgagccac tccccgacgc accgcacggc atgcgccgct tcgccgtcga
     5701 ccttcggctg cgggtcggcg gagagggctc gggcctggcc accttccgca aggccgcctt
     5761 cctggatctc ggagagccga tgcgcacgcc ccgcggatgg cgcgtggaga tcggctggca
     5821 ggcctccacc gccgcgccgc tgttcccggt gttttcggga gcgctggccg tcgaggatgg
     5881 cgtgatgcgg cttgagggcc tctacgcgcc gccgggcggc gtgctcggcc gcgtggccga
     5941 tcggctcctg ctgcacgtgg ccgccaacgg caccgcgcgc tggctgctcg acgagctcga
     6001 ccgggtggcg ctggagcgtg aggcctcggc gggctgagcc cggccctcag acccccggcg
     6061 gctccccgag gtggtgcttg gcgacgaacg ccgctgcctg cgtccggcgc tggaggttca
     6121 gcttggacag gatgctcgag acgtagttct tgaccgtctt gtcggagagg aagacctccg
     6181 acgcgatctc cttgttcgtc ttcccctcgg ccaccagcag caggatccgc cgttcctggc
     6241 tggtcagctc ggcgagctcg tcgctggcgc caccgctggc ggcggtacgc acccgctgca
     6301 gcaccttctc ggtcaccgcc gggtccagca gcgagtcgcc gcgcccgacc gattcgatcg
     6361 ccgcgaccag gtcgcgtccg cggatctgct tgagcaggta gcccgatgcg ccggcgatga
     6421 tcgcggacag caccgcctcc tcgtcggggt agctggtcag catgacgacc cgcgtctcgg
     6481 gccgggtgga gcggatctcg cggcacgcct cgatgccgga gccgtcgggc agccgcacgt
     6541 ccatgacgat cacgtcgggc tcgaagcgcg cggccatgct gatcgattcg gccacggtgc
     6601 cggcctgggc caccacctcg aacccctcgc gtcggtcgag gagcgcgacg atgccctgcc
     6661 gcaccacctc gtggtcgtcg acgaccagca ggcgcagcgg tcgctcgcgg gattcgtcgg
     6721 tcatggtcgc tcggtctccc ttgtgcccgc ggctcgtcgc gggatcgcga cgatgatacg
     6781 ggtcccgccc tccgtcgcgc gcgcaacctc gaacgtgccg cccagcgcct cgatgcgtgc
     6841 cctcatgttg ggcaggccgt ggtggcccct ggcggtcgcc gcctccgggt cgatgccgcg
     6901 accgtcgtcg acgatctcga ggcgcagctc ctccaccgcc gactgcacgg cgagcgaggc
     6961 gtgggaggcg gccgcatggc gcgcgatgtt gctcagcgcc tcgcgcgcga cggccagcag
     7021 ctcggcgacc agctcgatcg gcagctcgtc gagcccgccg gcggggtcct ccacgcggac
     7081 ctcgacgtcc accgccccgc tgcgtcgcag ctcggtggcc aggtggcgca gcccctcctc
     7141 caggctgccg ctctcgagca gcaccgggcg gaggccgaag atgaagttac ggatgtcgcg
     7201 gatcacgccg tggagcgcct cgatcgcgtc gtcgacgcgc cgggccgcct cctcggggtc
     7261 gtcctcgacc aggtccggca cgtcgtcgag ggcgagggtc tgggcgtaga tggactggat
     7321 cacgctgtcg tgcaggtcgc ggctgatgcg gtcgcgctcg tcgacgatcg ccaggcgtcg
     7381 ctcgtggtcc tgcaggcgcg cgttctggat ggcaatcccc gcgtgcaggg cgaacatctc
     7441 caccagccgc tcgtcgtcgg cggtgaactc ccgggcaccg cgcttgttgg tgaggtagag
     7501 ccggcccacc ggctcgccgc gcgccaggat cggcatgccc aggaaggcgt gcatctcggg
     7561 gtggtgtggc gggaagccgt agcgctcggg gtgggtggcg atgtccggga tgcggtacgt
     7621 ccggttgtcg cggatgatca gccccaggag gccccggccg cgcggcagct caccgatgcg
     7681 caggcggtgc tcgtggctga tgccgctggt gatgaagcgg atgatcgccc cgtcctcgtc
     7741 gacgatgccg agggcggcgt actcggcgtc ggcgagatcg cggacccggt cgacgatctc
     7801 ctgcaggacg cgctcgagct cccgctgcgc cccgatgccg cggaccgcca cgtcgagcgc
     7861 cgcgagctga cgggtggcgc gcgatgcgct ggcccgcccc tgcaccgcga ggaagacgaa
     7921 ctccagggcg ctgcggaacg cggccgcctg ctcggggtcg ccggccagct cgacggatcc
     7981 catgacggtg cccgcgtccg cgtccggcat gcggatcggc gttgcgaccg cgtcacgggc
     8041 accgaggtcc aggtgcagct cgggcagggg cggcgcaacg aggtcgaccc gcccgacggc
     8101 gaggcccagc ggctcgcaca cctcggcgat cgccgcgccc agcgcgtcgg cgcgcgtcgc
     8161 cgacggctcg tcgagccacg cctcgatggc gcggagcatc ggcgggagga gaagggtgcg
     8221 ccgggaggcc tcgggcgtgc tggtcatgcc ggagggaagc ctagcgcgcg acgtccgcca
     8281 gtgacacggc ccgcaggcga tcgaagacgg cctcctgagc cgcgaagaag gcggcatgca
     8341 cgtcgcattc gccgtcccgg cggcacgggc cgccgcgcag cacgcatcgg cgacggcgtc
     8401 cgttgccctc caccgcctcg accacggcga ggagcgagat gtccgacgcc tcacgcgcca
     8461 gcaggtagcc gccgtggcgg ccccgtcggt tctccaccag cccggcgcgc acgaggtcgc
     8521 ccatgacctg cgggacgtac gtcgccggga tggcggtcgc gtccgccagc gcggccccgg
     8581 tcagctgccc ggcgcccggc cgcgcgaggg tgagcatggc gcggacggcg tagtcgcctc
     8641 gtcgcgtgag ctcgattcgc atcggttctc cccttcagtc catgcggccc gagccacccg
     8701 ggacctatgg cacgtctcgc tgtccgccgg aatgtttaga acattactac acattctttg
     8761 gattcgaacg cgggagcaca ttcgagatgc agcgctactt cttctacggc ggcctcgccg
     8821 gcggtggatc ggccctcttc gtgagcctgg tcctcgcggg atcggtggtc gtcaacggcc
     8881 tgatgccggg ctcggcgacg ggggccaacg accccgcgac cgcgggcggc ggcatgaacc
     8941 acgacatgag cggcatgaac ggcggcggcg cgacggccgg cgtcaacggc acgctgaccg
     9001 aagcagaccc gaacgcgccg gcctacacgc cgcgcgacgc cacggcgccc gacctcatgg
     9061 agggcgaggt ccacgacatc gacctgtacg tcgtcgagca gcagatgacg gtggcggacg
     9121 gctacgcgca gaccgtctac gccttcgggt tcacgcccga gacggcgacc gtccccggtc
     9181 cggtgatccg ggtcaaggtc ggcgaccagg tccgcatcca cttcaagaac ccggcgacca
     9241 atgccatgcc gcactcggtc gacttccacg cctcgctggt ggcctggaac gacgagatga
     9301 ccgacatcaa cccgggtgag gagaagctgt acgagttcga ggccaagtac gcaggcgtgt
     9361 acatgtacca ctgcggcacc gccggtgcgc tgagccacat gctcaacggc atgtacggcc
     9421 tgatcatcgt ggagccggcc gagggcctgg acccggtcga ccaggaatac ttcctgatcc
     9481 agaacgagtg gtacatcggc cagcagggtg gcaccggcga cctcgagcgc gccaacgccc
     9541 ccgtcccgag ccccgacttc gtggtctgga acggcatcgc caaccagtac aaggacaacc
     9601 cgatccaggt gccggtcggt gagcagatcc gggtcttcgt cctgaacgcc ggaccgaaca
     9661 tcgacacctc gttccacgtg gtcggtacca tcttcaaccg ggtcatcaag gagggcgtcg
     9721 agctggcacg cggcaacgag gggaactggg gctcgcaggc ggtcgacctc gcgccggccc
     9781 agggtgccat cgtcgagttc aatttcgacg aggccggcct ctacccgatc gtgacccacg
     9841 ccttcaactt cgtggcccac ggcgccctcg gcctcttcca ggccggcgac ggcggagccg
     9901 gggcaggttc gggacactga tcgacatcgg accgatgccg gacggcggac acgccgccgt
     9961 ccggccacag caccgctccc gcgtgaccga gcgccacatc ctggcgctcg gtctcgcact
    10021 gtccggcacg ttcctggtcg cggccgtcag cgcggcgttc gtaacggtcg tcgccggggg
    10081 cagctcctgg gccgccctgc acctggcgct cgccggggcc gccggcgtcg ccatcggcgc
    10141 gttcatgccg cacttcgcca tcacgctggc cgggacggcg ccgcagccgg cgccgcagcg
    10201 cctggcgacc atcggtctcc tggccaccgg atccgctctg gccgtcgcgg gcgtcgccct
    10261 cctcggcggc cgggtggcgc tgggcggttc gatcctggtc gtcggcggcc tcgccctggt
    10321 cgggtggcag accgtggcgc cgctgcgcag cccgctggcg cgacggcacc cgatcgtgac
    10381 cgtcacgtac ctcctcgcgc tggcggagct ggcgaccggc gtcctgctgg gcggcttggg
    10441 cgccgctggc tgggaccccg tgacgcgggc atgggccgcc ctccgcccgg cgcacgcctg
    10501 gctgacgctg ctgggcgccg tgtcgctgac catcttcggc acgctgatct acctggcgcc
    10561 caccatcctg ggcgcccgaa tccgggccag ccggaccctg gcggtggcgg ccaccggcat
    10621 gctcgtcggg ccaccgctcg ccgcgctcgg cttcgcgctg ctcgcgacgc cggtcgtgct
    10681 cgccggcacc gtcctgacgc tggtcggcgc gctcgggcag atcagctacg tggtcgacac
    10741 gctccggcgg cgcggccgct acacgagtga gcacgactgg cggcgcgttg cggtgtggca
    10801 cctcgcggcg ggtaccggct ggttcgcggc cgccgtggcg gcggccctcg tcgggttcgt
    10861 cgacggcggg cggatcgccg cctggtccct cggggccctg gccgtcccgc tggtcgcggg
    10921 ctggatggcg caggagctgg tcggctcgtg gacgcacctc gccccgtcgg tgacgccggg
    10981 gagtcccggc cggcacgccg agcagcggcg cacgctggcc gtcgcgtcgc gggtaaggcc
    11041 catcgcctgg aacgtcggcg tgggactggc gtgggccggt gcggcgctgg gcgtgacgcc
    11101 gctggcggtg ggcggcggcg cgctgctcgc cgcctccgcg atcgcctcgg tcgcgctcct
    11161 gctgcgcgcg ctggcgcgcc agggggccgg ggtcaggccc ggggcatcgg ccccagcagg
    11221 gtgagggtct cgaccaggtc gacgccgagc ggctcgaggg cccgccgggc ggccaggtag
    11281 ggggagtgat cggcgccgac cgacggcccc gactccgtct ccaccgaggc gacccagccc
    11341 ttggcgaagc ggaccaatcg cagtggacgg cgcacgccgc gcaccaccac gaagcggcgg
    11401 atcacctcga aggtcggctc cgcggccggc gcgtcgagct cgggcgtgcg ggtcacgggt
    11461 accgcccccg gatctgctgt gcctcggcca cgcggcctac ggcgatggcg tgggccgcca
    11521 gccgggcatc gacgccttgg gcgtcgcgca tctcggccac ctggtcgtag gcacggccga
    11581 tgacgagccg cagtcgctcc gcgacctcgt cggccggcca ggcgaacgac tggaggttct
    11641 gaacccattc gaagtagctg acgatcaccc cgccggcgtt gcacaggatg tcgggcaccg
    11701 tcaccacgcc ccgctcggcc aggatcggat cggcgtccgg cgtgaccggg ccgttggcgc
    11761 cctcggccag caccctggcg cgaacggtgt gggcgttggc gccggtcacc tggccctcga
    11821 gggcggcgag gaccagcagg tccacgtcca gcgccaggag cgccgggttg tcgatcggct
    11881 gggttcccgg cgcgccggcg atggagccgg tctccttgag gtgcgccagc agccgccccg
    11941 ggtcgagccc gtcggggttc gcgacgccgc caccgacgtc ggtgatggcg acgagccgtc
    12001 caccgcgctc gtggatgagc cgcgcggcgg cctcgcccac gttgccgaag ccctgcacgg
    12061 cgacggtcgc gccgtccagg ctgacaccca gctcggccag cgcgcgctcg gccgtgaaca
    12121 tgacgcccat gccggtcgcg tccgcgcggc cctcggagcc gccgaggtcg accggcttgc
    12181 cggtgacgac gccgggaacg gtgtagccgg cgtgcatcga gacggtgtcc atcagccagg
    12241 ccatgacctg cgcgttggtg ccgacgtccg gcgccgggat gtcggtctcc ggaccgatga
    12301 ttccctgcag ctcggtcgcg aagcgccgcg tgacgcgctg cagctcgccc tggctcagcg
    12361 cgcgtggatc gatggtcacg ccgcccttcg cgccgccgaa cggcacgtcg accagcgcgc
    12421 acttccaggt catccacatc gccagcgccc ggacctcgtc caggtcggcg gccgggtgga
    12481 agcggatgcc gcccttggcc gggccgcggg agacgttgtg ctgcacacgg tagccggtga
    12541 ggacctccac gcggccgtcg tccatggtga ccgggaagtt gacggtccat tcgcgcttgg
    12601 ggacgcggag gatggcccgc gtctcggggg ccagctggag gtggtcggcg gcgcggtcga
    12661 actgggcgag ggcggaatcg agcgccgaga ggcggctcgc agctcgtccg cccgcctcga
    12721 cgcgcggatc ggtgacggtc atgaatgctc ctaggtgagg tggcgcgacc gggcacgggc
    12781 agcccggtcg catccgcatc gtcggtggcg cacatgacga acggcgtggc cgaacctgaa
    12841 cgggacgtga acggcggtat cctcgcggtg tgtcagcagc gcccctcgac gacctcctcg
    12901 cggtaccggt cgcggccctg ctggacgcac cacgacgccc cgacgaggtt gccgacctcc
    12961 tgggcgctga cgccatcaca gtcgcgccgg accggttgga gcggctcctc gagcggatga
    13021 cggcgctcgg gctggcacgg gtcgccggct acgacggcga gggacgggcc cggtacgtgg
    13081 tgacctcgct gggacagcgc gccgccgcgg cactgctcac ggcggagccc gagctccggg
    13141 tcggtctcga ggagctggag cgactgcgga ccgatctgct ggcgacggtc gggcacgacc
    13201 tgcgcacgcc actcaccgcc atccgcacct ccgtgggact gctcctcgat ccggccctcg
    13261 agccgtcgcc cgcacagcgc cagcagctgc ttacgaccat cggccgaagc gccgaccgca
    13321 tgcagcggct cctcacccag ctgctggacc tggcgcggct tcgcgccggg cgggtcgaga
    13381 tggatcgcac gcgcttcgac gcccgggaac tggtccacga ggtcgccgcc gccatcgagc
    13441 cgatggcgag cgctcgcagc cagcgcctca cgttggagct tccccccgag tccgtccagg
    13501 tcgtcggcga tcggcgctgg ctggagcagg ccctgctcaa catcgccgcc aacgcgcaga
    13561 agttctcgcc cagcggcgag gaggtgcgcg tcggggtgac cgcggacggc ggcacggtcc
    13621 ggtggtcggt gaccgaccgc ggccccggca tcgatgccgc ggcccaggcc cgactcttcg
    13681 agcgcttctt cgtgggggcg gccgaccggg gaggaggcgt cggcatcggc ctgcccaccg
    13741 cactgggcgt cgcgcacgcg cacggcggca ccatcgaggt cgacagcgag atcgggcggg
    13801 ggagcacgtt cacgctcatc gtgccccgcg cggacgacga cgcgtgaagc tgctgctggt
    13861 cgacgacgac gcggagatcc gggcatcggt ccggctcggc ttcgagctgc agtggcgtga
    13921 cgtcgagatc gtcgaggcgg cggcgggcgg cgaggcgctg gcggccgtgg agcgggagcg
    13981 ccccgacctc gtcctcctgg acgtcggact cccggacttc gacggctaca gcgtcctgcg
    14041 cgagatccgc gccttcagcg ccgtgccggt gatcatgctg accgcccgcg atcaacccat
    14101 cgacacggtg cgcggcctgg aggccggtgc cgacgactac gtcacgaagc cgttcgacca
    14161 cctcgagctg atggcccgcg tccgcgcggt gctgcgccgg ctcgacctgc gggcgcccgc
    14221 ctcgaggacc gctgcctatc gcaacggagc gctcgaggtg gacgtggagg cgcgcgaggc
    14281 gcgcgtggac ggtgagcgcg tcgcgctgac ggccagcgag tggcgcgtgc tggagctgct
    14341 ggtcgcgaac gcgggatggg tcgtccccta cgagcggctc acggcgcggc tgggcggtcg
    14401 ggacgagcca ggcgacctgg agggcctgcg ggtcttcgtg cgacggctgc gcctgaagct
    14461 cggcgacgac gctcatcggc cgcgctacat cgagacggtc cgcggtctcg gctaccggct
    14521 gctcgccgtc gcatgacgaa ccccttgcat ccacaccgct gcctgcgaga atgctccgga
    14581 ctccagcaca cgaggaaccg cccgtggaca tgctccgtcg atcggtgacc tggatcgcgc
    14641 tcctcgcggc cgtgatcctc gtctggctcc agctccccga ggactggccc atctcggcac
    14701 ccggcgaccc gctcaacggg tacctgacgt gggccttctg gatcctctcg atcctcgtgg
    14761 tcctcatcct cttcacgtac cgtcgtgatc cggcggccac cgaggtggag gtcgagggcc
    14821 cggcgttcgc ccggttcctg ttcgccaact cccgtgccgg gctgttctgg ctgcccgtgc
    14881 gcctcttcct gggcttcagc tggctcaccg cgagcctgca caagatcgcc gatggcggct
    14941 ggttggacgg cggggcctca ctggccggtt actggcagaa cgcggtctcg atccccgagg
    15001 gcgggcgtcc gccgatcacc tacgactggt accgcgactt catccagctc ctgctcaaca
    15061 acggcgcgga gagctggttc tcgtggctga tcgtcttcgg cgagctgctc gtcgggatcg
    15121 gcctcatcgt cggcgcgctc accggcatcg cggcgttctt cggcgccctg atgaacatgt
    15181 cgttcctgct tgccgggtcg gcgtcgtcga atccggtgct gttcaccgcg gccatcggcc
    15241 tgatgctggc atggcgcgtg gccgggtact acggcgtcga ccgctggctg ctgccgatgc
    15301 tcggcacgcc gtggcgggcc ccggtggcgc agcgcgcggc gaccggcgca ccgaatccgg
    15361 ggtagcgccg ggagcaaagc gaccgcacgg ccggggcgac ctgccccggc cgctccgtgt
    15421 cccagattcg aacgcttgtt ccaatccgac gttcgagcgt agactgcgcg gaccgtgacg
    15481 cgccgctcct ccgccgaccc gctcgccccc ttcagccagc ccacccgcac ctggttcgcc
    15541 gaggccttcg ccgggcccac gaaggcgcag gccctgggct ggcaggccat cagcggcgcg
    15601 aagcacgccc tc
//
LOCUS       DASTYP010000159         7295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_120753, whole genome shotgun sequence.
ACCESSION   DASTYP010000159 DASTYP010000000
VERSION     DASTYP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120753"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1044)
                     /locus_tag="VFV59_07350"
     CDS             complement(82..1044)
                     /locus_tag="VFV59_07350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5325682.1"
                     /translation="MAPMIGFHASHEQFRPDRLLRLVQQAEAAGFDAAMCSDHWAPWS
                     EAQGESGFAWSWLGAALQATSFSLGVVNAPGQRYHPAIIAQAAATLNVMYRDRFWVAV
                     GSGELMNEHITGEIWPNKADRNARLREAADIMRLLWAGETVSHSGYVTVSEARLWTWP
                     ERPPLLVGAAVTPETAGWVAEWADALVTVVQEPDTLDRVVDAFRSNGGEGKPMFLQFH
                     LAWAPSEEGAVTAAYDQWRQNTLPPSVMADLRHPAQFSTAAQHVRREDVAKAVRISSD
                     LGQHVEWLSAELQRGFDRVYLHEVGTEQERFVDTFGREVLPRLR"
     gene            complement(1046..2704)
                     /locus_tag="VFV59_07355"
     CDS             complement(1046..2704)
                     /locus_tag="VFV59_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEU5325683.1"
                     /translation="MSLEPTADLWWKNAVIYCVDVQSFLDSNGDGVGDLDGLTQRIDY
                     LAGLGVTCLWLMPIYPSPERDYGYDVADYYDVADKYGSLGDLVELVRTARERGIRVIA
                     DLVVNHTSIDHPWFQMSRKGHPVFKDFYVWEDEKPPNADEGVVFPGKQDSTWSYDRAA
                     RKWYMHRFYPFQPDLNINNPLVRNEIHKVIGFWLELGLSGFRIDAVPFLIEQVHGDHP
                     AVKDPHDYLRDLRDFAIRRRGDTMLLAEANEPLDRIAGFFGERGNQMNMLFSFVTNQA
                     LYLSFVRGEAAPVADALRALPPVPPEAQWANFIKNHDEATLDRLTDAERQEVFAAFAP
                     DESMRLYGRGIRRRFASMVNGDRRRLELAYSLLFSLPGTPVLFYGEEIGMADNPDIPD
                     RGAVRNPMQWADQPGGGFTTADERKLPRPLMPDGPFGYGEVNVREQRRDPDSFLNWTE
                     KAIRTRKEWPEFGWGEWRILGTRNPHVLAHVATWDGASVLALHNFGDAPAKVTVRIPE
                     EATGGRWQHIFGRGDEDPEGIEGDTFSCELGPYGYHWYGRREGV"
     gene            3013..3816
                     /locus_tag="VFV59_07360"
     CDS             3013..3816
                     /locus_tag="VFV59_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5325684.1"
                     /translation="MPDPVQLHVHEWTSERPNGRTAVLVHGMNGWHRTWWRVGPALAG
                     RGWRVLAVDQRGHGRSPRTGQAASLDDVAEDLAAAIERHAMPPIDLLVGHSLGAAVSL
                     WLVHLRPDIARRLVAEDPPSIDRSADEEFFAKVRADIAAAREHPQEELRREIAENPRW
                     MEEDARQDVEGRAMADADGIVASLRWPRPFRVVDLAPQLQTPTLFILADPQRSVMLPE
                     ARTALPGRLPAHSRVIEMDAGHTVHRDRFDEYLAAILAWADQGTEQGRA"
     gene            3813..4571
                     /locus_tag="VFV59_07365"
     CDS             3813..4571
                     /locus_tag="VFV59_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEU5325685.1"
                     /translation="MSSLDDVGAWLRHARSVVALTGAGISTESGIPDFRGPNGLWTRD
                     PASERLSNIGYYVADASIRRESWRRRAEHPAWTAEPNAGHRALARLEQAGRLELLITQ
                     NVDGLHLAAGTSPERLIEIHGTIRETACLGCGDRRPMLEVIERVRAGEPDPPCELCGG
                     ILKSATVSFGQNLDPTLLSRAEEAAAACDLFLAIGTSLTVYPVARLPELAVEFGARLV
                     IVNAEPTPLDERADAVLRGSSGDILERLVSQVVG"
     gene            4582..5478
                     /locus_tag="VFV59_07370"
     CDS             4582..5478
                     /locus_tag="VFV59_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5325686.1"
                     /translation="MEHLTIRDVPGIRVGHWTDATAATGCTVILPADGGAVAGVDVRG
                     SAPGTRETDLLRPTALVERVNAICLAGGSAFGLAAADGVVRRLAERGIGFPTAVRPVP
                     IVPAAILFDLGVGDPNAFPTADAGYAATLTAEADEGPLEGRVGAGTGATCAKLGGTGA
                     GTPAGVGSAAQRLPDGSTVGALVVLNALGDVFARDGRPLATATGSTPPPVVNTTLVVV
                     ATDASLDRAQCRKAAELVHDALAIGIRPVHTMFDGDVAFALNTGDGSRMAPEPFFGLG
                     RAVVDVVGEAIERSLGVGVKSA"
     gene            complement(5482..6345)
                     /gene="panB"
                     /locus_tag="VFV59_07375"
     CDS             complement(5482..6345)
                     /gene="panB"
                     /locus_tag="VFV59_07375"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5325687.1"
                     /translation="MTETRPREPLTIPEIGAMKRAGTKIVMVTAYDHPSARLASEAGV
                     DIILVGDSAGNNVLGYETTVPVTMDQAVMLTEAAARARPRSLLVGDLPFGSYQASDAD
                     AVRNAARLVKAGADAVKLEGAGPMLSRVRAIVGAGIPVMGHVGLTPQSATMLGGMKAQ
                     GRTAADARRMLDDARELEAAGCFSLVLESVPARIGEHISRALRIPTIGIGAGPGCDGQ
                     VLVWHDLLGINTGPMPRFVKRYADVGGEIRRGLEAFASDVRSGAYPADEHEYKISREE
                     LDAFEADLRGG"
     gene            complement(6377..>7295)
                     /locus_tag="VFV59_07380"
     CDS             complement(6377..>7295)
                     /locus_tag="VFV59_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325688.1"
                     /translation="LAWGAVASVLEGPTEADGFTWYRLEADDGATGWAAAGDAEDDWL
                     VPVLVPSEADLRIQVSSACDVVGPLATPDTTVLEDGLVVTSTFSGGYVAGVLSAEGMG
                     SLETEILSNPYLQQDGDYVPVPRAGAGEPPGHGACTYTFVVGSGDNPVRVSSVSWFGD
                     AEESQFWEPAPERRALTQIAQSLSDIESVLPSTAWTAEPLPYVAENYVMWLIAESGPM
                     AGSFVEIADLNLAASADAFGDAVTDISRCGVLSREQARRVHAGLRAAGFESGLAGFVG
                     GPMRDGDSGYSLLIAPQTPVGEPGCDGLR"
BASE COUNT         1043 a         2626 c         2553 g         1073 t
ORIGIN      
        1 agccggcgcc gcggagggcc gggcaagggc cgcaactcgt gagttacttg ccgccccggc
       61 aagtcacgcc tcgcggcgac cctaccgaag ccggggaagc acctcgcggc cgaaggtgtc
      121 gacgaaccgc tcctgttccg ttccgacctc gtggaggtag acgcggtcga agccacgctg
      181 cagctcggcc gacagccact cgacgtgctg cccgagatcc gacgagatgc gcacggcctt
      241 cgccacgtcc tcccgacgaa cgtgctgcgc cgccgtcgag aactgcgcgg gatgccgcag
      301 gtccgccatg acggatggcg gcagcgtgtt ctgccgccac tggtcgtagg cggcggtcac
      361 cgccccctcc tcagagggtg cccaggcgag gtggaactgc aggaacatcg gcttaccctc
      421 gccgccgttg gagcggaagg catcgacgac gcggtccagg gtgtcgggct cctgcacgac
      481 cgtgacgagt gcatcggccc attcggcgac ccagcccgcg gtctcgggcg tgaccgcggc
      541 gccgaccagc aggggcgggc gttccggcca cgtccacagg cgagcctccg acaccgtgac
      601 gtacccgctg tgcgacaccg tctcgccggc ccacagcagc cgcatgatgt cggcggcctc
      661 gcgcagtcga gcgttgcgat ccgccttgtt gggccagatc tcgccggtga tgtgctcgtt
      721 catgagctcg cccgagccga cggcgaccca gaagcggtcg cggtacatca cgttcagggt
      781 tgcggcggcc tgggcgatga tcgcggggtg gtagcgctgg ccgggcgcat tcaccacgcc
      841 gagcgagaac gaggtcgcct gcagggccgc gccgagccac gaccacgcga agccggactc
      901 gccctgggcc tcgctccacg gcgcccagtg gtcggagcac atggccgcgt cgaagccggc
      961 ggcctccgcc tgctgaacca gccgcagcag ccgatccgga cggaactgtt cgtgactggc
     1021 gtggaagccg atcatcggag ccatctcaca cgccttcccg ccggccgtac cagtggtagc
     1081 cgtacggccc cagctcgcag ctgaacgtgt cgccctcgat cccttcgggg tcctcgtcgc
     1141 cccggccgaa gatgtgttgc cacctcccgc cggtcgcctc ctcggggatc cggacggtca
     1201 ccttcgccgg cgcgtcgccg aagttgtgga gggccaacac gctggcaccg tcccaggtcg
     1261 ccacgtgagc gaggacgtgc gggttccgcg tgcccaggat ccgccactcg ccccagccga
     1321 actccggcca ctccttgcgc gtgcgaatcg ccttctccgt ccagttcagg aacgagtcgg
     1381 ggtcgcgtcg ctgctcacgc acgttgacct cgccgtagcc gaacggcccg tccggcatca
     1441 acggccgcgg cagcttccgc tcgtcggcgg tggtgaaccc gccaccgggc tgatcggccc
     1501 actgcatggg gttgcggacc gcgccgcggt ccggaatgtc cgggttgtcc gccatgccga
     1561 tctcctcgcc gtagaacagc accggcgtac cgggcaggct gaagaggagg ctgtaagcga
     1621 gctccagtcg gcggcgatcg ccgttgacca tggacgcgaa gcggcggcgg atgccgcggc
     1681 cgtagaggcg catcgactcg tcgggggcga aggcggcgaa gacctcctga cgttccgcat
     1741 cggtcaatcg gtccagcgtc gcctcgtcgt ggttcttgat gaagttggcc cactgcgcct
     1801 ccggcgggac gggcggcagc gcgcgcagcg catcggccac cggggctgcc tcgccgcgca
     1861 cgaacgacag gtacagcgcc tggttggtga cgaagctgaa gagcatgttc atctggttgc
     1921 ctcgctcgcc gaagaagccg gcgatgcggt ccagcggctc gttggcctcc gctagcagca
     1981 tggtgtcgcc ccggcgtcgg atcgcgaagt cgcgcaggtc acgcagatag tcgtgtgggt
     2041 ccttgacggc cgggtggtcg ccatggacct gctcgatcag gaacgggacg gcgtcgatcc
     2101 ggaatccgga caggcccagc tccagccaga agccgatgac cttgtggatc tcgttccgca
     2161 cgagcgggtt gttgatgttg agatcgggct ggaaggggta gaaccgatgc atgtaccact
     2221 tccgcgccgc gcggtcgtag ctccaggtgc tgtcctgctt gccggggaag acgacgccct
     2281 cgtcggcgtt cggcggcttc tcgtcctccc acacgtagaa gtccttgaag acggggtgcc
     2341 ccttgcggga catctggaac caggggtggt cgatcgaggt gtggttgacc accaggtccg
     2401 cgatcacccg gatgccccgc tcccgcgccg ttcgcaccag ctcgacgagg tcgccgaggc
     2461 tgccgtactt gtcggcgacg tcgtagtagt cggccacgtc gtagccgtag tcccgctcgg
     2521 gtgaggggta gatcggcatc agccagaggc aggtgacgcc gaggcccgcg aggtagtcga
     2581 tccgctgggt caggccgtcc aggtcgccga ccccgtcacc gttcgagtcg aggaacgact
     2641 gcacgtccac gcagtagatg accgcattct tccaccacag gtcggcagtt ggttccaggc
     2701 tcatggcctg gagcacttgc acgtggcgtt ccggcgctcg ctggacgggc gagcgtttgg
     2761 cggtctgcgg gtgcgtccgt gcgtcggtgc gtcgcgatcc atcgcggcgc ttgcgcgaga
     2821 aacggttctc ctgccccgga ttcgcgattg atcgaggact gtccggcgca caccgggggc
     2881 gttgggtgct gcaggtgccc acaggttcgc gatgcatcga gaaaccggaa ccgtccggcg
     2941 tccttccgtg cgcccgccgc ggtggatcgc gacggtggag cgagcggcga acccggtcgg
     3001 gataatgccc ccatgccaga ccccgttcag ctgcacgtcc acgagtggac atccgagcgc
     3061 cccaatggcc gcaccgcggt cctcgtccac ggcatgaacg gctggcaccg cacctggtgg
     3121 cgcgtgggtc cggccctcgc cgggcgcggc tggcgcgtgc tcgccgtcga ccagcggggg
     3181 cacgggcgaa gcccgcgcac gggacaggcg gcgtcgctgg atgacgtcgc ggaagacctc
     3241 gcggccgcca tcgaacgcca cgccatgccg ccgatcgacc tgctggtcgg ccattcactg
     3301 ggggccgcgg tcagcctctg gctggtgcac ttgcgacccg acatcgcgcg gcggctggtg
     3361 gcggaggatc cgccgagcat cgatcgcagc gccgacgagg agttcttcgc gaaggtccgg
     3421 gcggacatcg cggcggcccg cgagcacccg caggaggagc tccgtcgcga gatcgcggag
     3481 aacccccggt ggatggagga ggatgcccga caggacgtcg agggccgggc catggccgat
     3541 gccgacggca tcgtcgcgtc gctgcgctgg ccgcgcccct tccgggtcgt cgacctcgcc
     3601 ccacagctcc agacgccgac cctgttcatc ctcgccgacc cacaacgctc ggtcatgctc
     3661 cccgaggccc gcaccgccct gcccggccgc ctgccggccc actcccgggt catcgagatg
     3721 gacgctggcc acacggtcca tcgcgaccgc ttcgacgagt acctcgcggc gatccttgcc
     3781 tgggctgacc agggcacgga gcagggccgc gcgtgagctc cctcgacgac gtcggggcct
     3841 ggctgcggca cgcgcgctcc gtcgtcgccc tcactggggc gggcatcagc accgaatcgg
     3901 gcatcccgga cttccgcggt ccgaacggcc tctggacgcg cgacccggcc agcgagcggc
     3961 tgagcaacat cggctactac gtggccgatg ccagcatccg tcgcgagagc tggcgccggc
     4021 gtgcggagca cccggcctgg accgcggagc cgaacgccgg gcaccgggcg ctggcccggc
     4081 tcgagcaggc aggtcgcctc gagctcctga tcacccagaa cgtcgacggg ctgcatttgg
     4141 cggccggcac gtcaccggag cggctgatcg agatccacgg gaccatccgg gagaccgcct
     4201 gcctcggctg cggcgaccgc cggccgatgc tcgaagtcat cgagcgcgtc agagcgggcg
     4261 agccggatcc gccgtgcgag ctgtgcgggg gcatcctcaa gtccgcgacc gtgagcttcg
     4321 ggcagaacct cgaccccacg ctgctgtcgc gcgcggagga ggcggccgcg gcctgcgatc
     4381 tgttcctcgc catcggcacc agcctcaccg tctacccggt ggcgcgcctg cccgagctgg
     4441 ccgtcgagtt cggcgcgcgc ctcgtcatcg tcaacgcgga gccgacgcca ctcgacgagc
     4501 gcgccgatgc ggtcctgcgt ggcagctcgg gcgacatcct cgagcgcctg gtgtcgcagg
     4561 tggtcggcta gcatcggtcc cgtggagcac ctgaccattc gcgacgtgcc cggcatccgg
     4621 gtcggccact ggaccgatgc gacggccgcc accggctgca ccgtgatcct ccccgccgac
     4681 ggcggggccg tcgccggcgt cgatgtccgg ggttccgcgc ccggcacgcg cgagaccgac
     4741 ctcctgcgcc cgaccgcctt ggtcgagcgt gtcaacgcca tctgcctggc cggcgggtcg
     4801 gccttcggcc tggccgctgc cgatggcgtc gttcgacgcc tggcggagcg gggcatcggc
     4861 tttccgacgg cggtccgccc ggtcccgatc gttccggccg cgatcctctt cgacctcggt
     4921 gtcggcgacc cgaacgcctt tccgaccgcc gatgccggct acgccgcgac cctcaccgcc
     4981 gaggcggacg aaggaccgct cgagggccgc gtcggtgcgg gaaccggcgc cacctgcgcc
     5041 aagctcggtg ggacgggcgc cgggacaccg gcaggcgtcg ggagcgccgc gcagcggctg
     5101 cccgatggct cgaccgtcgg cgcgctggtc gtgctcaacg ccctcggcga cgtgttcgcc
     5161 cgtgacggcc ggccgctcgc gacggcgacc ggcagcaccc cgccaccggt cgtcaacacc
     5221 acgctcgtcg tcgtggccac cgacgcgtcg ctggatcgag cgcagtgtcg caaggccgcg
     5281 gagctggtcc acgacgcgct ggccatcggc atccgcccgg tccacaccat gttcgacggc
     5341 gacgtggcgt tcgcgctcaa caccggcgac ggcagccgga tggcgccgga gccgttcttc
     5401 gggctgggtc gggcggtggt ggacgtggtg ggcgaggcga tcgaacggag cctcggcgtg
     5461 ggcgtcaaga gcgcctgagg ttcagccccc gcgcagatcg gcctcgaagg cgtcgagctc
     5521 ctcgcggctg atcttgtact cgtgctcgtc ggccgggtac gcaccgctcc ggacgtcgga
     5581 ggcgaacgcc tccagcccgc gccggatctc gccgccgacg tcggcgtagc gcttcacgaa
     5641 gcgaggcatc ggcccggtgt tgatgcccag gaggtcgtgc cagacgagca cctgcccatc
     5701 gcagcccgga ccggccccga tgccgatggt cgggatgcgc agtgcacggc tgatgtgctc
     5761 gccgatgcgc gcgggcaccg actccagcac cagcgagaag cagccggccg cctcgagctc
     5821 gcgcgcgtcg tcgagcatgc ggcgggcatc cgccgccgtg cggccctggg ccttcatccc
     5881 accgagcatc gtcgcggact gcggggtcag gccgacgtgg cccatcaccg ggatgccggc
     5941 accgacgatg gcgcgcaccc gcgacagcat cggtccggcg ccctcgagct tcaccgcatc
     6001 ggccccggcc ttcaccaggc gggccgcgtt gcggaccgca tcggcgtccg aagcctggta
     6061 ggagccgaag ggcaggtctc cgaccagcag cgagcggggg cgagcgcgtg cggctgcctc
     6121 ggtcagcatg accgcctggt ccatggtgac cgggacggtc gtctcgtagc cgagcacgtt
     6181 gttcccggcg gaatcaccaa ccaggatgat gtcgacgccc gcctccgacg ccagccgcgc
     6241 ggacggatgg tcgtacgccg tgaccatcac gatcttggtg ccggcgcgct tcatggcgcc
     6301 gatctcgggg atggtcaagg gctcgcgcgg gcgggtctcg gtcatgcgcg aagcgtcgcc
     6361 ctcgcgtatc ggcttgtcaa cgcagcccgt cgcagcccgg ttcgccgacc ggggtctgcg
     6421 gcgcgatgag gagcgagtag ccgctgtcgc catcccgcat gggtccgccg acaaaccctg
     6481 ccaggccaga ctcgaacccg gccgcacgca ggccggcgtg gacgcgacgt gcctgctcac
     6541 ggctcagcac gccgcagcga ctgatgtcgg tcaccgcgtc gccgaaggcg tcggcgctgg
     6601 cggcgagatt caggtccgcg atttcgacga acgaccccgc catcggtccc gactccgcga
     6661 tcaaccacat gacgtagttc tcggcgacgt acggcagtgg ctcggccgtc caggccgtcg
     6721 agggcaggac cgattcgatg tcgctgaggc tttgggcgat ctgtgtcagg gccctgcgtt
     6781 ccggcgcggg ctcccagaac tggctctcct ccgcgtcccc gaaccagctg acagagctca
     6841 cccgtaccgg gttgtcgccc gaaccgacca cgaatgtgta ggtgcacgca ccgtgtcccg
     6901 gtggctcccc ggcaccggcg cgaggcaccg ggacgtagtc cccgtcctgt tggagatacg
     6961 ggttcgagag gatctcggtc tcgagcgagc ccatgccctc ggccgacagc acgcccgcaa
     7021 cgtacccccc gctgaaggtg ctggtgacga ccagcccgtc ctcgaggacg gtcgtgtcgg
     7081 gcgtcgccag aggccccacc acgtcgcacg cgctgctgac ctgaatgcga agatcggcct
     7141 ccgacggcac gagcaccggg acaagccagt cgtcctccgc gtcgccggcg gccgcccacc
     7201 cggtggcgcc atcgtccgcc tcgaggcgat accaggtgaa gccgtcggcc tcggtcggcc
     7261 cctccaggac cgatgcgacc gctccccagg ccagc
//
LOCUS       DASTYP010000160         6229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_813499, whole genome shotgun sequence.
ACCESSION   DASTYP010000160 DASTYP010000000
VERSION     DASTYP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813499"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2259
                     /gene="priA"
                     /locus_tag="VFV59_07385"
     CDS             <1..2259
                     /gene="priA"
                     /locus_tag="VFV59_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU5325689.1"
                     /translation="PGSLLLVPYGRRLALGYLLPPDATSAGLAPELRTVEAVVSEPML
                     TPDLLALAEEIAVYYRAPVGTTLAAMLPPGLESRLVRRWHVADAGELPPGARPLVDAD
                     GWITDAALVRHAPRRGRTAWLEGLRRRGVVTADWSLRPPSVTERRVRIVRRLQGGDEP
                     LRRAPRQRAILDALGDGELTMAELADRLGGDAASLLAPARRLEALGRVELGWRSVERD
                     PLAHRSTHEPLRHTLAQEQRAALEAIETLPSGGELLLQGVAASGKTDVYLAAIQATIE
                     AGGGAIVLVPETSLIPQLGDRLRALLGDQLAVVHSGLSAGERHDEWWRILRGDARVVV
                     GTRTAAFAPLPDLRLIIVDEEHDGGYKSDRTPRYDARWVARRRAALVGARVVFGSATP
                     DLVTLARVRGGHAERAHLAARRVGEAPSVEVVDMRSELSSGNRSIFSAPLAEALGALR
                     RGREQAVLLINRRGAATFVLCRDCGESLRCPDCDLPFVFHLAGATLRCHHCGRTAATT
                     ETCPSCGSRRIRYFGAGTQRVEAELQGRFPGLRVGRLDSDALAARRGFEAVYDDFREG
                     RIDVLVGTQLAAKGLDLPAVTLAAVVAADVTLNLPDYRAAERTFQLLAQVAGRAGRGP
                     DPGRVIFQTYAPSHYAVRSAAALDVDAFADEELLRRRLLGYPPFSVLARLLVADPDRR
                     TAEARGREAADAVAAPGVDVLGPLPSYVPRRAGRYRFQVVLRAPDTDARAAALERVPP
                     RVAIDVDPESLL"
     gene            2281..2787
                     /gene="def"
                     /locus_tag="VFV59_07390"
     CDS             2281..2787
                     /gene="def"
                     /locus_tag="VFV59_07390"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5325690.1"
                     /translation="MVIRRILTAEEPILRERTKKVSTFDASLHRLLEDMLETMRDAPG
                     VGLAANQIGVPLQVAVIEVDGKVTELVNPQVVKSSGEQTDWEGCLSIPGFVAEVTRAA
                     KVTVKARDRHGKEFRVKGEELFARALQHEIDHLNGSLYIDYLDSLEELVRVSEAPDEV
                     EEETAAGI"
     gene            2787..3743
                     /gene="fmt"
                     /locus_tag="VFV59_07395"
     CDS             2787..3743
                     /gene="fmt"
                     /locus_tag="VFV59_07395"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU5325691.1"
                     /translation="MGRIGRIAFLGTGAFGVPLLAHVIDAADDLLVVSQPDRPAGRRL
                     HLRATPVAALARERGVRVMTPSRLRADEAKEAIRDFAPDGLLLVAYGQLVPRDLLALG
                     ARPPLNVHPSLLPRHRGAAPVAGTILAGDTEGGVTLMVMTPELDAGPIVAQWPVTLTG
                     RETTPELEAQLSDLAAEVVPPELERWACGELTMTPQDDRAATHVHPFTRADGWIDWRR
                     SAVDIDRQVRALQPWPGAWTSLDDRRVHVRRARPVAGVDAVPVGALLPGDVPRVACGI
                     GALELEVVQPEGRPTMPADAWRRGLSRDHVLLGAGRPEEALR"
     gene            complement(3760..4302)
                     /locus_tag="VFV59_07400"
     CDS             complement(3760..4302)
                     /locus_tag="VFV59_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5325692.1"
                     /translation="MTEEARGRARLAARGRRGPGWMGALALGAVGAGLGAVAAMLLDP
                     ARGRTRRARLVDQSAAVLRRAGREVQRETRRVGAAVEGRMAAIRAAGEHDGADPGNDA
                     TIADRVQSALFADPSIPKGDLNVNVEHGIVVLRGEVPDDAMRRQLIERAQRIDGVWSV
                     RDLMHLPGETAPTDVHSLAR"
     gene            4481..5206
                     /locus_tag="VFV59_07405"
     CDS             4481..5206
                     /locus_tag="VFV59_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEU5325693.1"
                     /translation="MFFDPIYIFAVLIPTLAITGWASWRVRSTYSKWSKVDSGINLDA
                     FDFARRLLDRQGLHDVKIEPTPGQLTDHYDPRGKVLRVSSQVADASALETMGLRSAAP
                     RLSVAAAAVIAHEVGHALQDQQREPMMALRQAIVPAAQFGSSIAPWLVIAGVIFQFTG
                     LAIVGLIGFAGAVLFTFATLPVEIGASGKALAFVNGLGMVGERQDGARAVLKAAAWTY
                     VAGALTALLTFLYYVFLIFGRRD"
     gene            5199..>6229
                     /gene="rlmN"
                     /locus_tag="VFV59_07410"
     CDS             5199..>6229
                     /gene="rlmN"
                     /locus_tag="VFV59_07410"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5325694.1"
                     /translation="MTELAIAGPRPAISEIDPATLAAWLTARGEPPYRASQILAGVHR
                     PQVGGFEDLSDLPRGLRAALAGDFRFSAIVDSHVIGADGGLTAKAVHELHDGQRIESV
                     LMRYPSRTGATRTTICISSQAGCAVACPFCATGQAGFGRQLTPGEIVDQVLHWHRPPW
                     SALADGEGHYNIVFMGMGEPLNNADRVFAAVRLLNDPHRLGIGARHITVSTSGVVPAM
                     DRMVDELPQVNLAISLHAATDALRDELVPINRKWPVADVIAAGRRFAGRTGRRVSLEY
                     VMIDGVNDTEAQASRLVELARGWRSHVNLIPLNPTPGSRWSGTSPAGIARFVAILRGG
                     GVAVTVRDTR"
BASE COUNT          771 a         2300 c         2284 g          874 t
ORIGIN      
        1 ccgggcagcc tgctgctcgt gccgtacgga cgccgcctcg ccctcggcta cctgctcccg
       61 cccgatgcga cgtccgcggg gctcgcgccg gagctcagga ccgtcgaggc ggtggtctcg
      121 gagccgatgc tgacgccgga cctcctcgcc ctggccgagg agatcgccgt ctactaccgc
      181 gcgccggtgg gcaccacgct ggccgcgatg ctgccaccgg gcctcgagtc gcggctggtc
      241 cgccgctggc acgtcgccga tgccggcgag ctgcctcccg gggcgcggcc gctggtcgac
      301 gccgacggct ggatcaccga tgcggcgctg gtgcgtcacg cgccgcggcg cgggaggacc
      361 gcgtggctgg aggggcttcg ccggcggggc gtggtgaccg ccgactggtc gctgcgtcca
      421 ccgagcgtga ccgagcgtcg cgtccggatc gtccgccgcc tccagggcgg cgacgagccg
      481 ctccgccgcg cgccccggca acgggcgatc ctcgacgcgc tcggcgatgg ggagctcacc
      541 atggcggagc tggcggatcg gctcggcggg gacgcggcat cgctcctcgc gccggcccgt
      601 cggctggagg cgctggggcg cgtcgagctc gggtggcgga gcgtcgagcg cgatccgctc
      661 gcgcaccgct cgacgcacga gccacttcgc cacaccctgg cgcaagagca gcgcgccgcg
      721 ctggaggcga tcgagaccct cccgtcgggc ggagagctgc tgctgcaggg ggtcgcggca
      781 tcaggcaaga ccgacgtcta cctcgcggcg atccaggcca ccatcgaggc gggcgggggc
      841 gccatcgtgc tcgtccccga gacgagcctg atcccgcagt tgggcgaccg tctccgtgcc
      901 ctgctggggg accagctcgc ggtcgtgcat tccggcctgt cggcggggga gcggcacgac
      961 gagtggtggc ggatcctgcg cggcgacgca cgggtcgtgg tggggacgcg gacggcagcc
     1021 ttcgcgccgc tgcccgacct ccggctgatc atcgtggacg aggagcacga cggcggctac
     1081 aagtccgaca ggacgccgcg ctacgacgcg cgctgggtcg cacggcgccg tgccgcgctc
     1141 gtcggcgcac gggtcgtctt cggctccgcg acgcccgatc tggtgacgct cgcccgcgtg
     1201 cgtggcggcc acgccgagcg ggcgcacctc gcggcccgcc gggtcgggga ggcgccgagc
     1261 gtcgaggtgg tcgacatgcg cagcgagctg tcgtccggca atcgctcgat cttctctgcg
     1321 ccgctggccg aggcgcttgg ggcgctccga cgcgggcgag agcaggcggt gctgctcatc
     1381 aaccgccgtg gggcggcgac cttcgtcctg tgccgcgact gcggcgagtc gctgcggtgt
     1441 cccgactgcg acctgccgtt cgtcttccac ctggccggcg ccacgctgcg ctgccatcac
     1501 tgtgggcgca ccgcggcgac gacggagacg tgccccagct gcggcagccg ccggatccgc
     1561 tacttcggcg cggggacgca gcgggtcgag gcggagctgc agggccgctt cccggggctg
     1621 cgggtcggcc ggctcgactc ggacgccctg gcggcgcgcc gggggttcga ggcggtctat
     1681 gacgacttcc gcgagggtcg catcgacgtc ctggtcggga cgcagctggc cgccaagggc
     1741 ctcgacctgc ccgcggtcac gctggcggcg gtcgtggccg ccgatgtcac gctgaacctg
     1801 cccgattacc gggcggccga gcgcaccttc cagctgctgg cccaggtcgc gggccgcgcg
     1861 ggacgggggc cggatcccgg ccgcgtgatc ttccagacct acgcaccgtc ccactacgcg
     1921 gtccgcagcg cggcggcgct cgacgtcgat gccttcgccg acgaggagct cctgcgccgc
     1981 cgtctgctcg gctacccacc cttcagcgtc ctggcgcgtc tcctcgtcgc ggacccggac
     2041 cgccgtaccg ccgaggcgcg cgggcgcgag gccgccgacg ccgtggcggc acccggcgtg
     2101 gacgtcctcg ggccgcttcc gagctacgtg ccgcggcgtg ccggacggta tcggtttcag
     2161 gtggtcctgc gtgcgccgga caccgatgcg cgagccgcag cgctcgagcg cgtgccgccc
     2221 cgcgtggcca tcgacgtgga ccccgaatcg ctcctgtgag gtggctacaa tggcgcggcc
     2281 atggtcatcc gccgcatcct caccgccgag gaacccatcc tcagggaacg cacgaagaag
     2341 gtctcgacct tcgacgccag cctgcatcga ctgctcgagg acatgctcga gacgatgcgt
     2401 gacgcgcccg gtgtcgggct cgccgcgaac cagatcggcg tcccgctgca ggtggcggtg
     2461 atcgaggtcg acggcaaggt cacggagctc gtcaacccgc aggtggtgaa gtcgtccggc
     2521 gagcagaccg actgggaggg ctgcctctcg atcccggggt tcgtggccga ggtgacccgc
     2581 gcggccaagg tcacggtcaa ggcgcgggac cggcacggca aggagttccg cgtgaagggc
     2641 gaggagctgt tcgcgcgggc gctccagcac gagatcgacc acctgaacgg ctcgctctac
     2701 atcgactacc tcgactccct cgaggagctc gtccgcgtct cggaggcccc cgacgaggtg
     2761 gaggaagaga cggccgccgg catctagtgg gacgcatcgg ccgcatcgca ttcctgggga
     2821 cgggcgcctt cggcgtgccg ctgctcgccc acgtcatcga cgccgcggac gacctgctcg
     2881 ttgtgagcca gccggaccgc ccggccggcc gcaggctcca cctccgggcg acgccggtcg
     2941 cggcgctcgc tcgtgagcgc ggcgtgcggg tcatgacgcc gagccggctg cgcgccgacg
     3001 aggccaagga ggcgatccgc gacttcgccc cagatgggct cctgctcgtc gcctacggcc
     3061 agctcgtccc acgcgacctc ctggcgctcg gcgcgcgccc gcccctgaac gtccacccgt
     3121 cgctgcttcc ccgccaccgc ggtgcggcgc cggtcgcggg caccatcctg gccggcgaca
     3181 ccgagggcgg tgtgacgctg atggtgatga cgcccgagct ggatgccggg ccgatcgtgg
     3241 cgcagtggcc cgtcaccctg accgggcgcg agacgacgcc cgagctcgag gcgcagctgt
     3301 cggacctcgc ggccgaggtc gtaccgcccg agctggagcg ttgggcgtgt ggcgagctga
     3361 ccatgacgcc gcaggacgac cgcgccgcga cgcacgtcca cccgttcacg cgggccgatg
     3421 gctggatcga ctggcgtcgg tcggccgtcg acatcgaccg gcaggtccgc gccctccagc
     3481 cgtggcccgg cgcctggacg agcctcgacg accgccgcgt ccacgtccga cgcgcgcgtc
     3541 ccgtcgcggg cgtcgacgcg gtcccggtgg gcgcgctgct gcccggtgac gtgccgcgcg
     3601 tggcgtgcgg catcggcgcg ctggagctgg aggtcgtgca gcccgagggc cgtccgacca
     3661 tgcccgcgga cgcgtggcgc cgcgggctgt cccgggacca cgtcctgctg ggggccggac
     3721 ggccggagga agcgctccgc tgaggagcgc ccggcgcggc tagcgcgcca ggctgtggac
     3781 gtcggtcggt gcggtctcgc ccggcaggtg catcaggtcc cggacgctcc agacgccgtc
     3841 gatgcgctgc gcccgctcga tgagctgccg gcgcatggcg tcgtccggca cctcgccccg
     3901 gagcaccacg atgccgtgct cgacgttgac gttgaggtcg cccttcggaa tcgagggatc
     3961 ggcgaagagc gcgctctgca cgcggtcggc gatggtcgcg tcgttgcccg gatctgcgcc
     4021 gtcatgctcg ccggccgccc ggatggccgc catgcggccc tccaccgcgg cgccgacgcg
     4081 gcgcgtctca cgctggacct cgcgtccagc ccggcggagc acggcggcgg actggtcgac
     4141 caggcgtgct cgccgggtac gaccgcgcgc cggatccagc agcatggccg ccacggcgcc
     4201 gaggcccgcg ccgacggctc ccagggccag cgcccccatc cagccggggc cacggcgacc
     4261 gcgagcggcc agccgggcac gtccgcgggc ctcctcggtc acggcttcgc ttcgatcaag
     4321 gattcttgtc aggaggtcca tcggtccttc ctctgcgtat cgtctgatgc gttcaggcac
     4381 gcatgcagcg tgcccggcac cgacctggac ggccggggaa tgggggagcg ttgggtcgga
     4441 tatactcgtc cgagccctca cggaggtggg caacacaccg atgttcttcg acccgatcta
     4501 catcttcgcg gtgctgatcc cgaccctggc catcaccggc tgggcgtcgt ggcgcgtccg
     4561 ctccacgtac agcaagtggt ccaaggtcga ctccggcatc aacctcgatg cctttgactt
     4621 cgcgcgccgg ctcctggatc gccaggggct gcacgacgtg aagatcgagc cgaccccggg
     4681 ccagctcacc gatcactacg acccgcgcgg gaaggtgctg cgcgtcagca gccaggtcgc
     4741 ggacgcgtcg gccctcgaga cgatgggcct gcggtcggcg gcgccgcgcc tctcggtggc
     4801 cgcggcggcg gtcatcgccc acgaggtggg ccacgccctg caggaccagc agcgggagcc
     4861 gatgatggcc ctgcgccagg cgatcgtgcc cgcggcccag ttcggctcga gcatcgcgcc
     4921 gtggctggtc atcgccgggg tcatcttcca gttcaccggg ctcgccatcg tcggcctcat
     4981 cggcttcgcc ggcgccgtgc tcttcacgtt cgccaccctg ccggtggaga tcggggcatc
     5041 gggcaaggcc ctggccttcg tcaatggcct gggcatggtc ggggagcggc aggacggcgc
     5101 tcgagcggtc ctcaaggccg ccgcctggac ctacgtggcc ggcgcgctga ccgcgctgct
     5161 cacgttcctg tactacgtgt tcctgatctt cggccgacgt gactgagctc gcgatcgccg
     5221 ggccgcggcc cgccatcagc gagatcgacc cagcaacgct cgccgcgtgg ctcaccgccc
     5281 gcggcgagcc gccgtaccgg gcctcgcaga tcctcgccgg cgtgcatcgg ccgcaggtcg
     5341 gcgggttcga ggacctcagc gacctcccgc gtggcctccg cgcggcgctg gccggcgact
     5401 tccgcttcag cgccatcgtc gacagccacg tgatcggtgc cgatggcggc ctgaccgcca
     5461 aggccgtcca cgagctgcac gacggccagc ggatcgagtc ggtcctgatg cgctacccgt
     5521 cccgtaccgg cgcgacgcgc accacgatct gcatcagctc ccaggccggc tgcgccgtgg
     5581 cctgtccgtt ctgcgcgacc ggccaggcgg gcttcgggcg ccagctgacg cccggcgaaa
     5641 tcgtcgacca ggtgctgcac tggcaccggc cgccgtggtc ggcgctggcc gacggcgagg
     5701 ggcattacaa catcgtgttc atggggatgg gggagccgct caacaacgcc gatcgggtct
     5761 tcgcggccgt gcggctgctc aacgacccgc atcggctggg catcggcgcc cgtcacatca
     5821 ccgtgtcgac ctcgggcgtg gtgcccgcca tggatcgcat ggtggacgag ctgccgcagg
     5881 tgaacctggc catcagcctg cacgcggcaa ccgatgcgct gcgcgacgag ctggtgccga
     5941 tcaaccgcaa gtggccggtc gccgacgtca tcgccgccgg tcggcggttc gccggccgga
     6001 caggccggcg cgtgagcctc gagtacgtga tgatcgacgg cgtcaacgac accgaggcgc
     6061 aggccagccg cctcgtggag ctggcccgcg gctggcgctc ccacgtcaac ctgatcccgc
     6121 tcaatccgac gccgggctcc cgatggagcg gcacgtcacc ggccggcatc gcccgcttcg
     6181 tcgcgatcct gcgcgggggc ggcgtggcgg tcaccgtgcg cgacacccg
//
LOCUS       DASTYP010000161         5634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_959241, whole genome shotgun sequence.
ACCESSION   DASTYP010000161 DASTYP010000000
VERSION     DASTYP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959241"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFV59_07415"
     CDS             complement(<1..143)
                     /locus_tag="VFV59_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131981.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU5325695.1"
                     /translation="MRTPVVVDGLRTPFGRYGGALKDIRPDDMAAHVIRALLDRTAVD
                     PSL"
     gene            complement(155..754)
                     /locus_tag="VFV59_07420"
     CDS             complement(155..754)
                     /locus_tag="VFV59_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEU5325696.1"
                     /translation="MTFDVSETVALARWWWTFILRGLLAIAFGVLAFFAPAWGIVILV
                     TLFAAWAFVDGVASLFTAIRTRETDRHWWLELVEGLVSILAGIVALLLPVDAVARVLV
                     LVIAVWAILTGALEIWAAIRLRERIQGELLLGLAGLSSILFGIVLFLFPGAGALSLVW
                     LIGGFAIAFGVFLIALGWRLRRIDELAKRDAAHDYGSAG"
     gene            complement(751..975)
                     /locus_tag="VFV59_07425"
     CDS             complement(751..975)
                     /locus_tag="VFV59_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU5325697.1"
                     /translation="MCRSIKTLRTGAEPASDDEITAAALQYVRKVSGYRKPSAANEPA
                     FDAAVAEVTAASKRLLEAVAATHPQARRAS"
     gene            complement(1000..1653)
                     /locus_tag="VFV59_07430"
     CDS             complement(1000..1653)
                     /locus_tag="VFV59_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEU5325698.1"
                     /translation="MSVPFGGRYRAVVFDMDGLLLDTEVLWHEAESELFRRHGGEFTW
                     DDKMTVIGTSFGFTADYFADRLGRPREEGPALVEEMIGLMHDLVRHQVDARPGAVELV
                     TRLRQAAGMRLGLASNSPRFLVDDALRTAGLADAFDAIVTSDDVEHSKPAPDIYLLAC
                     ERLGVAPSEALALEDSASGVAAAKAAGLMCIAVPQFAETDVTAADRVVDSLESLIES"
     gene            complement(1650..2861)
                     /locus_tag="VFV59_07435"
     CDS             complement(1650..2861)
                     /locus_tag="VFV59_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5325699.1"
                     /translation="MTEPHEDVRGVGVLGAGTMGAGIVQVAAEAGFEVLVHDPVAGAV
                     ERATERIAGFLRRKVEKGQLPPGSDEGALARIRDVARLEELAAADLVIEAAPEDLELK
                     RDVFARLDGAAPPTTLLATNTSSLSVASIAAAAEHPERVIGMHFFNPVPLMALVEVIA
                     GPMTAEWAVDRVVLTGRRFGKTPVVAADTPGFIVNRVQRAYYLEAFRIYEEGLASVDG
                     IDAAMRGIGFRLGPFELADVVGTDVNLAAGTQIFEGFFGDPRYRPAQVQRRVADAGRL
                     GRKTSAGYYDYAEDGTHGGAWAALQRPSLLPPLQPAQIEARVLAAIVNEAASAVADGV
                     ARPEAIDTAMRLATNWPEGPLAWGERIGLSSVVHTLDALHAAVPDGRYRVTPLLRHLA
                     SEGGSFFVHPA"
     gene            complement(2858..3646)
                     /locus_tag="VFV59_07440"
     CDS             complement(2858..3646)
                     /locus_tag="VFV59_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5325700.1"
                     /translation="MPDATGLEVLRTDRDGAVAILTLNRPESLNALTGELRRALLTAV
                     KDAGREESVRAVVVTGAGRAFCAGADLRAGAGEREFRRVLTSEYNPLVEAMRGLPKPI
                     VAAVNGVAAGAGFSLAMAADLVVASADARFVPAFHRIGLVPDSGLARTLVRAIGRHRA
                     LEILLGERELSATDAEQLGLVARVVPAEGLQDAALELARRLAAGPTRGIGMTKRLLNA
                     AEDASLADALGAEAALQELAGRTEDHAEGVAAFAEKRAPEFRGR"
     gene            complement(3728..3994)
                     /locus_tag="VFV59_07445"
     CDS             complement(3728..3994)
                     /locus_tag="VFV59_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5325701.1"
                     /translation="MSIIAWLVVGALAGWIAGMVVKGDEGYGVIGTIIAGIVGALVGG
                     FILGAVTGEDWTTGINIPTLVAAIVGAIIVVFLWGYFARGRRTV"
     gene            complement(4110..5072)
                     /locus_tag="VFV59_07450"
     CDS             complement(4110..5072)
                     /locus_tag="VFV59_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325702.1"
                     /translation="MGGLGNPDHAVGGLAVPRPAGVELRPLGRDDFEVALSLVRELYG
                     LPAGDDAVLRPRYDAFVNDVDVASFLALADGEPAGLIGFRFRRRLNHATFEGWVSDLV
                     VREPFRRRGVGRALLGAAIAEWRLRGGHQVMLEVAYQRTAARALYEAMGFVHRGKVFE
                     MNPVRSRGIVPGPAVEIREIRDDDTDFDATTRLLAELGRPAPTEERLPALRRTYAQHV
                     ARADTSSRLARLDGEPVGFISLEFREPFFMTRSQAWIPDLIVTERARGRDIGAALLDA
                     AFVEAQARNAYAATLESGDQRLVAHRLYTTAGMADVGSFFTLIR"
     gene            complement(5073..>5634)
                     /locus_tag="VFV59_07455"
     CDS             complement(5073..>5634)
                     /locus_tag="VFV59_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5325703.1"
                     /translation="NATPIDMLRTNRIGQWDRIRRITKPLIAAVNGWALGGGCELAMT
                     LDLIVAGEDAKFGQPEINIGVIPGAGGTQRLTRAIGKSKAMRMVLTGEPITAHEAEAA
                     GLVAVVTQDELVVEDALALAATMAQKSPIALRVAKEAVNAAYEMSLTDALAHERRLFY
                     LLFASEDQKEGMAAFLEKRSPDFTGR"
BASE COUNT          851 a         2073 c         1971 g          739 t
ORIGIN      
        1 atcaacgacg ggtccaccgc ggtccggtcg agcagggcgc ggatgacgtg cgccgccatg
       61 tcgtcgggtc ggatgtcctt cagcgcgccg ccgtaccggc cgaagggggt tcgcaggccg
      121 tcgacgacga ccggcgtgcg caacgggtcg cggctcaccc ggccgacccg tagtcgtgcg
      181 ccgcgtcgcg cttcgcgagc tcgtcgatcc gccgcaggcg ccagccgagc gcgatgagga
      241 agacgccgaa ggcgatcgcg aagccgccga tgagccacac caggctgagc gcgccggccc
      301 ccgggaagag gaacagcacg atgccgaaca ggatcgacga gaggccggcc agcccgagca
      361 gcagctcccc ctggatccgc tcgcggagcc ggatggcggc ccagatctcg agcgcgccgg
      421 tcaggatcgc ccagacggcg atcacgagga ccaggacgcg cgccaccgcg tcaaccggca
      481 gcaggagggc cacgataccg gcgaggatcg agacgagacc ctccaccagc tcgagccacc
      541 agtgccggtc ggtctcacgg gtgcggatgg cggtgaagag gctggcgacg ccgtcgacga
      601 aggcccacgc agcgaacagg gtgaccagga tcacgatccc ccacgcgggc gcgaagaagg
      661 cgaggacgcc gaaggcgatc gccagcagcc cgcggaggat gaaggtccac caccagcgag
      721 cgagcgcgac ggtctcggag acgtcgaacg tcatgaggct cgccgcgcct gggggtgcgt
      781 ggcggccacg gcctcgagca gtcgcttgga ggcggcggtc acctccgcca cggccgcatc
      841 gaacgccggc tcgttggccg ccgatggttt gcggtagccg ctcaccttgc gcacgtactg
      901 gagtgcggcg gcggtgatct cgtcgtcact ggccggctcg gcgccggtcc ggagggtctt
      961 gatgcttcgg cacatgcggc gaggataccc cctgccggcc tacgactcga tcagcgactc
     1021 gagcgagtcc acgacgcggt cggccgcggt gacatcggtc tcggcgaact gcggcactgc
     1081 gatgcacatc agccccgccg ccttggccgc ggcgaccccg gacgccgagt cctcgagcgc
     1141 cagcgcctcc gacggcgcca ctcccagccg ttcgcacgcc agcaggtaga tgtccggcgc
     1201 cggcttcgag tgctcgacgt cgtccgaggt gacgatcgcg tcgaatgcat cggccaggcc
     1261 tgccgtgcgc agcgcgtcgt ccaccaggaa gcgcggtgag ttcgacgcca gccccagccg
     1321 catgccggcg gcctggcgca accgggtgac gagctccacg gcaccgggtc gcgcgtcgac
     1381 ctggtgccgc accaagtcgt gcatcaggcc gatcatctcc tccaccagcg cgggtccctc
     1441 ctcacgtggt cggcccaacc gatccgcgaa gtagtccgct gtgaacccga agctcgtccc
     1501 gatcacggtc atcttgtcgt cccaggtgaa ctcgcccccg tggcggcgga agagctcgga
     1561 ctccgcctcg tgccacagca cctccgtgtc gagcaggagc ccgtccatgt cgaagacgac
     1621 cgcgcggtac cggccaccga acgggacgct cacgccggat ggacgaagaa cgagccgccc
     1681 tcggacgcga ggtgccgcag cagcggcgtg acgcggtagc gaccgtccgg gacggccgcg
     1741 tgcagcgcat ccagcgtgtg gacgacgctc gacagaccga tgcgctcgcc ccaggcgagc
     1801 ggcccctcgg gccagttcgt ggcaagccgc atcgccgtgt cgatggcctc cggccgggcc
     1861 acgccgtccg ccacggccga ggcggcctcg ttgacgatgg ccgccagaac gcgagcctcg
     1921 atctgcgccg gctggagtgg aggaaggagc gaggggcgct gcagcgcggc ccacgcaccg
     1981 ccgtgggtgc cgtcctcggc gtagtcgtag tagcccgcac tcgtcttgcg tcccagccgg
     2041 cccgcatccg ccacccgccg ctgcacctgc gccggtcggt agcgcggatc gccgaagaag
     2101 ccctcgaaga tctgggtgcc cgcagccagg ttgacgtccg tcccgaccac gtcggccagc
     2161 tcgaagggcc ccagtcggaa gccgatgccg cgcatggcgg catcgatccc gtcgaccgat
     2221 gccagccctt cctcgtagat gcggaacgcc tccaggtagt acgcgcgctg cacgcggttg
     2281 acgatgaagc ccggcgtatc ggccgccacg accggggtct tgccgaagcg ccggccggtg
     2341 aggaccacgc ggtcgacggc ccactcggcc gtcatcggtc cggcgatgac ctcgaccagc
     2401 gccatcagcg gcaccgggtt gaagaagtgc atgccgatca cgcgctctgg atgctcggcc
     2461 gcggccgcga tgctcgcgac ggacagggag ctggtgttgg tggccaggag cgtcgtgggc
     2521 ggcgccgcgc cgtccaggcg agcgaagacg tcgcgcttca gctcgaggtc ctctggggcc
     2581 gcctcgatga cgaggtccgc ggcggcgagc tcctccagcc gggcgacatc ccgaatgcgt
     2641 gcgagggcgc cttcgtcgga gccgggaggg agctgcccct tctcgacctt ccggcgcagg
     2701 aacccggcga tgcgctccgt ggcccgttcc accgccccgg ccaccgggtc gtggacgagc
     2761 acctcgaacc cggcctccgc cgcgacctgc acgatgccgg cacccatcgt tccggcgccg
     2821 agcacgccga cgccgcggac gtcctcgtgg ggctcggtca tcgaccgcgg aattccgggg
     2881 cgcgcttctc ggcgaaggcg gccacaccct cggcgtggtc ctcggtgcgg cccgccagct
     2941 cctggagcgc ggcctcggcg cccagcgcgt cggccagcga cgcgtcctcg gccgcgttca
     3001 gcagccgctt ggtcatcccg atgccgcgcg ttggtcccgc cgccaggcgc cgcgccagct
     3061 ccagtgccgc gtcctgcagc ccctcggccg gaacgacacg agccaccagg ccgagctgct
     3121 cggcatccgt tgcggagagc tcgcgctcgc ccagcaggat ctcgagggca cggtgccggc
     3181 cgatcgccct gacgagcgtg cgcgccagcc cggagtcggg gacgagcccg atgcggtgga
     3241 aggcggggac gaaacgagcg tcggcggagg cgaccaccag gtcggcggcc atggccaggc
     3301 tgaacccggc gccggccgcg acgccgttga cggcggcgac gatcggcttg gggagaccgc
     3361 gcatcgcctc gaccagcggg ttgtactcgc tcgtcagcac gcggcggaac tcccgctccc
     3421 cggcgccggc gcgcaggtcg gctccggcgc agaaggcccg cccggctccg gtcacgacga
     3481 ccgcgcgcac cgactcctcg cggccagcat ccttcaccgc ggtcagcagg gcacgacgga
     3541 gctcgccggt gagcgcgttg agcgactccg gacggttgag cgtcaggatc gcgacggcgc
     3601 cgtcgcgatc ggtccggagg acctcgaggc cggtggcgtc aggcatggac cgcgattgta
     3661 ccgaggcccg gacaccgaaa cgcccggcgc gacagcggcg ccgggcgcgg gttcagcgac
     3721 gaatcgatca gacggttcgg cggccgcggg cgaagtagcc ccacaggaag accacgatga
     3781 tcgccccgac gatggccgcg acgagggtcg ggatgttgat cccggtcgtc cagtcctcac
     3841 cggtcacggc tccgaggatg aagccgccga ccagggcgcc gacgatgccg gcgatgatcg
     3901 tgccgatcac gccgtagccc tcgtcaccct tgacgaccat gccggcaatc cagccggcaa
     3961 gcgctcctac gaccaaccac gcaatgatgc tcatgaatac ctccgtgtcc ctctctcttt
     4021 ctccaggcgg caatcgagtc cgccacgcgg aagcatgcac gttccgtacc ggtccgcgcc
     4081 agcactttgt acgagcgctt ggtcgcccct cagcgaatca gggtgaagaa gctgcccacg
     4141 tccgccatgc cggccgtcgt gtagagccga tgcgccacca gccgctggtc gccgctctcg
     4201 agcgtcgccg catacgcgtt gcgggcctgg gcctccacga acgcggcgtc cagcagagcg
     4261 gcgccgatgt cgcgaccgcg cgcgcgctcg gtcacgatga ggtccgggat ccacgcctgc
     4321 gacctggtca tgaagaacgg ctcccggaac tccaggctga tgaagccgac cggctcgccg
     4381 tcgagccggg cgagccgcga cgacgtatcg gcgcgggcca cgtgctgcgc ataggtccgc
     4441 cgcagcgccg ggaggcgctc ctcggtcggc gccggccgcc caagctcggc gagcagcctc
     4501 gtggtcgcgt caaagtccgt gtcgtcgtcc cggatctctc ggatctccac ggccggcccg
     4561 ggcacgatgc ctcgtgagcg gacggggttc atctcgaaca ccttgccccg atgcacgaag
     4621 cccatcgcct cgtacagggc gcgggccgcg gtccgctggt aggccacctc gagcatcacc
     4681 tggtggccgc cgcgcagccg ccactcggcg atggccgcgc cgagcagcgc acgaccgact
     4741 ccacggcggc ggaacggttc gcggaccacc aggtcgctga cccagccctc gaacgtggcg
     4801 tggttcagcc gtcgccggaa gcggaacccg atcaggcccg ccggctcgcc gtcggccagg
     4861 gccaggaacg aggcgacatc cacgtcgttg acgaacgcgt cgtaccgcgg gcgcagcacc
     4921 gcgtcgtcgc cggcgggcag cccatagagc tcgcggacca gggacagcgc gacctcgaag
     4981 tcgtcgcgac ccagcggccg cagctcgacc ccggccggcc gtggcaccgc caggccgccg
     5041 accgcgtggt cggggttgcc gaggccgccc acctatcggc ccgtgaagtc ggggctgcgc
     5101 ttctcgagga aagcggccat cccctccttc tggtcctcgg acgcgaagag gaggtagaag
     5161 aggcggcgct cgtgcgccag cgcatcggtc aggctcatct cgtaggcggc gttgacggcc
     5221 tccttcgcga cgcggagcgc gatcgggctc ttctgcgcca tggttgcggc caacgccagc
     5281 gcgtcctcga cgaccagctc gtcctgggtc acgacggcca ccaggcccgc ggcctcggcc
     5341 tcgtgcgccg tgatcggctc gccggtcagc accatgcgca tcgccttgct cttcccgatg
     5401 gcacgggtca gccgctgcgt tccgcccgcc ccggggatga ccccgatatt gatctcgggc
     5461 tgcccgaact tcgcgtcctc gcccgccacg atcaggtcga gggtcatcgc cagctcgcag
     5521 ccgccgccca gggcccagcc gttgaccgcc gcgatcagcg gcttggtgat cctccggatg
     5581 cggtcccact ggccgatgcg gttcgtgcgc agcatgtcga tgggggtggc gttg
//
LOCUS       DASTYP010000162         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1760613, whole genome shotgun sequence.
ACCESSION   DASTYP010000162 DASTYP010000000
VERSION     DASTYP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760613"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFV59_07460"
     CDS             complement(<1..558)
                     /locus_tag="VFV59_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325704.1"
                     /translation="MGLFNRHLRLLFLVTALVVAAFSTGIDFLFFLVYLAAGIGIGSW
                     LYARRGLRGVRADYRVLNPRTHVGEVLQAMYRVENHDRFGKPWIECWNESTLVNSLPG
                     RVVGMSGRGTRQWLAKVTLTRRGTYRLGPLRVRSGDPFGLFSAEMAVGQPTGVVVFPE
                     VVPLPHWRLPPSPIDGTTRTKRRYEA"
     gene            complement(580..1554)
                     /locus_tag="VFV59_07465"
     CDS             complement(580..1554)
                     /locus_tag="VFV59_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5325705.1"
                     /translation="MKNVQQPAERVVASVERVIVGKHVEVRMALVALLCEGHILIEDV
                     PGVGKTMLAKALAQSIGCSFRRIQFTPDLLPSDVTGLSIFNQKTTEFEFRPGPIMAQV
                     VLADEINRATPKTQSALLECMEERQATIDGVTYPMPSPFLVIATQNPIEYEGTFALPE
                     AQLDRFMLRLRLGYPKAMEEIVILDEQKRRHPIASVEQVLGLEELREMQAAIKEIYVD
                     QAVAEYIVRLVHATRDHPDVYLGASPRGSISLYRATQAYAALAGRDYVIPDDVKHLAV
                     AILAHRLIVKSQASLREVDPEQIVSEILASVPVAEAAAAAETGRPRVS"
     gene            complement(1692..3230)
                     /locus_tag="VFV59_07470"
     CDS             complement(1692..3230)
                     /locus_tag="VFV59_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5325706.1"
                     /translation="MIATNVRRLGFYLILAFALVSGSMVWWQVIEAPALASRSDNPEV
                     IAARRSLPRGTIFDASGQVLASSSLVGDLSVRSYADPAFTHVIGYSSLRFGSTGLERA
                     YEDLLVGRADPNPIRDLLNDVLARTPQPRDLTLTIDRRLQDFAAAELGTDPGAVVAID
                     PTTGAILALVSTPTFDATPISGDPDAAQEPMDALRADPAQPLLPRARQGTYVPGSIMK
                     VFTGVAALDAGAITPETTFSDQPAQETQGFPVQGFTIREHDLGGIQPALWPLSEALQV
                     SSNIFFAHVGLELGGDQFLAYARRFGFCDPMAIGPDDRALSVAASYVTNPVEGDCTPF
                     DDDVELASASFGQGEVVVTPVQMALLAAAVAGDGVMPDPYVVRDVRAHSDTGTPSDTV
                     LDRFASGGGTRVVSQGAARAMRAAMVDAVNGELGRLYAGQGDITLYGISGSVSAGKTG
                     TAQLGGEQAPHSWFIGFAPAQEGAAPSIAVAVLVESGGSGSGRAAPIAGRVMAEYLRM
                     TGGN"
     gene            complement(3227..4564)
                     /locus_tag="VFV59_07475"
     CDS             complement(3227..4564)
                     /locus_tag="VFV59_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU5325707.1"
                     /translation="MRIAWRGAELRLLLPILLLVPLGFLVTNVAVTGTFEPGPMQLAA
                     VYVGLFVVAHLVLVLSGHRGDQLLLPAVGAMGGIGIVMLNRLPQDLAGTSALGFELGM
                     AATQVLWFGVGLVAMLVVAIGLRDDGILRHYKYSWAAIGILLLAATMLVGYEVNGARL
                     WIDLGPVSVQPGELIKITLVVFLAAYLAETRTLLTSARVRIGFISIPPLPYFLPMLAL
                     FGVVMLIAVRLNDLGTALLFFGTFLVMLFVATGRRSYVLIGLLLFVAGSFVAYELFGH
                     VQARINVWLDPFADPLGAGFQPVRALYALGRGGIFGEGLGQGLVTLGGNLTIPFVHTD
                     FIFTAIAEELGLLGGLALLAFAAVLVFRGLRIAALARDDFSALLAVGLTASLGLQTLI
                     IIAGNTKLVPLTGITLPFVSYGGSSVLASFLMIGLLLSISHRTQIGTDAERGE"
     gene            complement(4575..>4926)
                     /locus_tag="VFV59_07480"
     CDS             complement(4575..>4926)
                     /locus_tag="VFV59_07480"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5325708.1"
                     /translation="LLRDLRSAEEAQMASRSGIGRLSVLESPEDEPPAGSTIALGPIN
                     SIGRNVNNTIYVEDDFASATHAMLTFRGRSWFVEDQGSTNGTYVNGHRIDRPVALSFG
                     DEITVGRVRMRLDR"
BASE COUNT          788 a         1678 c         1781 g          679 t
ORIGIN      
        1 cgcctcgtac ctgcgcttgg tgcgcgtggt gccgtcgatc ggtgatggcg gcagacgcca
       61 gtgcggcagc gggaccacct cgggaaagac gacgacgccg gtcggctggc cgacggccat
      121 ctcggcgctg aagaggccga atgggtcacc ggagcggacg cgcagcggcc ccaggcggta
      181 cgtcccgcgc cgcgtcagcg tcaccttggc cagccactgt cgggttccgc gacccgacat
      241 gccgacgacg cggccgggaa ggctgttcac cagcgttgat tcgttccagc actcgatcca
      301 cggcttgccg aagcggtcgt ggttctcgac ccggtacatc gcctggagca cctcaccgac
      361 gtgcgtccgc ggattcagga cgcggtagtc ggcgcgcacc ccgcgcagcc cgcgtcgcgc
      421 gtacagccac gacccgatcc cgatgccggc ggccaggtag acgaggaaga acaggaagtc
      481 gatgcccgtg ctgaacgccg ccaccacgag cgcggtgacg aggaagagca gtcgcaggtg
      541 ccggttgaac aggcccatgg cgcgccgtgc ggtcccgcgc tacgagacgc gcgggcgacc
      601 ggtctccgcc gcggcggccg cctcggccac ggggaccgag gcgaggatct cgctgacgat
      661 ctgctccggg tccacctcgc gcagcgaggc ctggctcttc acgatcaggc ggtgcgccag
      721 gatggccacg gccaggtgct tcacgtcgtc ggggatgacg tagtcgcgtc ccgccagcgc
      781 cgcgtaggcc tgcgtcgcgc ggtacaggct gatcgagccg cgcggcgagg cacccaggta
      841 gacgtccggg tgatcgcgcg tcgcgtgcac cagccgcacg atgtactcgg ccaccgcctg
      901 gtcgacgtag atctccttga tcgcggcctg catctcgcgc agctcctcga ggcccagcac
      961 ctgctcgacc gacgcgatgg ggtggcggcg cttctgctcg tcgaggatga cgatctcctc
     1021 catcgccttg gggtagccca ggcgcaggcg gagcatgaac cggtcgagct gcgcctccgg
     1081 cagcgcgaag gtgccctcgt actcgatggg gttctgggtg gcgatgacga ggaacgggga
     1141 cggcatcgga taggtcacgc cgtcgatggt ggcctgtcgc tcctccatgc actcgagcag
     1201 cgcgctctgt gtcttcggcg tggcgcggtt gatctcgtca gcgaggacca cctgggccat
     1261 gatcggcccc gggcggaact cgaactcggt cgtcttctgg ttgaagatcg agaggcccgt
     1321 gacgtcggag ggcagcaggt ccggcgtgaa ctggatgcgc cggaaggagc agccgatgct
     1381 ctgggcgagc gccttggcga gcatcgtctt gcccacgccg ggcacgtcct cgatgaggat
     1441 gtggccctcg cacaggagcg cgacgagagc catccggact tcgacgtgct tgccgacgat
     1501 cacccgctcc acgctggcga ccacgcgctc ggcgggctgc tggacgttct tcatgtcagg
     1561 agtggtcctc tccccgtgtg gcgacgagcc cggccctgcg ggcccgacct tgtgctcaac
     1621 aatactcccc gccgttacgc gggcgttcat tcgtcactgg gggaccctcg ccagacgcga
     1681 tgatggagcg gtcagttccc gcccgtcatg cgcaggtatt ccgccatgac ccggccggcg
     1741 atgggtgccg cacggcccga gcccgacccg ccactctcga cgagcacggc aacggcgatc
     1801 gacggcgccg ctccctcctg tgccggcgcg aagccgatga accacgagtg cggcgcctgt
     1861 tccccgccga gctgcgccgt tcccgtcttg ccggccgaga cgctgccgct gatgccgtag
     1921 agcgtgatgt cgccctgccc ggcgtacagt cgaccgagct ccccgttcac ggcgtcgacc
     1981 atggcggcgc gcatcgcgcg ggcggcgccc tggctcacca cgcgcgtccc tcccccagag
     2041 gcgaatcggt cgagcaccgt gtcgctcggc gtgcccgtgt cgctgtgggc gcggacgtcg
     2101 cgcacgacgt aggggtcggg catgacgccg tcgccggcga cggccgcggc aaggagcgcc
     2161 atctggacgg gcgtgaccac cacctcgcct tggccgaagg aggcggacgc gagctctacg
     2221 tcgtcgtcga agggggtgca gtcgccctcc accgggttcg tgacgtagct cgccgcgacc
     2281 gagagggcac gatcatcggg gccgatggcc atcgggtcgc agaagccgaa gcgtcgcgcg
     2341 taggccagga actggtcgcc gccgagctcc agcccgacgt gggcgaagaa gatgttgctc
     2401 gagacctgca gcgcctccga cagcggccac agcgccggct ggatcccacc caagtcgtgc
     2461 tcgcggatgg tgaagccctg gacgggaaag ccctgggtct cctgcgccgg ctggtcggag
     2521 aaggttgtct cgggcgtgat ggcgccggcg tcgagtgccg cgacgccggt gaagaccttc
     2581 atgatcgagc ccgggacgta cgtgccctgg cgcgcgcgcg gcagcagcgg ctgggcggga
     2641 tcggcgcgga gcgcgtccat gggctcctgg gcggcatccg ggtcgccgga gatgggggtc
     2701 gcgtcgaagg tcggggtcga gacgagcgcc aggatggcgc cggtggtcgg gtcgatggcc
     2761 acgaccgctc ccggatcggt gccgagctcc gcggccgcga agtcctgcag gcgacggtcg
     2821 atggtcagcg tcaggtcgcg cggctgcggg gtgcgggcca ggacgtcgtt cagcagatcg
     2881 cgaatcgggt tcgggtcggc ccggccgacc agcaggtcct cgtacgcgcg ctccaggccg
     2941 gtgctgccga agcggagcga ggagtacccg atgacgtggg tgaaggccgg atcggcgtag
     3001 ctgcggaccg acaggtcccc gaccagggac gacgaggcca ggacctggcc cgatgcgtcg
     3061 aagatggtgc cgcgcgggag gctgcgacgc gccgcgatga cctcggggtt gtcgctgcgg
     3121 ctggcgagcg ccggggcctc gatcacctgc caccacacca tgctgcccga cacgagcgcg
     3181 aaggccagga tgaggtagaa gcccaggcgc cggacgttgg tcgcgatcac tcgccacgct
     3241 ccgcatcggt gccgatctgg gtgcggtgac tgatcgacag cagcaacccg atcatcagga
     3301 agctggcaag gacgctcgag ccgccgtagc tgacgaaggg cagcgtgatg ccggtcagcg
     3361 gcaccagctt cgtgttgccg gcgatgatga tcagcgtctg cagccccagg ctggccgtca
     3421 ggccgaccgc cagcagcgcg ctgaagtcat cgcgcgccag cgccgcgatc cgcaggccgc
     3481 ggaacaccag gaccgccgcg aaggccagca gcgcgaggcc gccgagcagc cccagctcct
     3541 ccgcgatggc cgtgaagatg aagtcggtgt ggacgaacgg gatggtcagg ttgcccccga
     3601 gcgtgaccag gccctgcccc aggccctcgc cgaagatgcc gccgcggccc agcgcgtaca
     3661 gggcccgcac cggctggaag cccgcgccca acggatcggc gaacgggtcc agccagacgt
     3721 tgatgcgcgc ctggacgtgg ccgaacagct cgtacgcgac gaagctccca gccacgaaca
     3781 ggagcagccc gatcagcacg tagctgcgcc gcccggtcgc gacgaacagc atcaccagga
     3841 aggtgccgaa gaagagcagc gcggtgccca ggtcgttgag gcggacggcg atgagcatga
     3901 ccacgccgaa caaggcgagc atcggcagga agtacggcaa cggcgggatg gagatgaagc
     3961 cgatgcgcac gcgcgcgctg gtcagcaggg tccgcgtctc ggccaggtag gccgccagga
     4021 agacgacgag cgtgatcttg atgagctcac cgggctgcac gctcaccggt ccgaggtcga
     4081 tccacagcct ggcgccgttg acctcgtagc cgacgagcat ggtcgcggcc agcaggagga
     4141 tgccgatcgc cgcccacgag tacttgtagt gccgcaggat gccgtcgtcg cgcaggccga
     4201 tggcgaccac cagcatcgca accagcccga cgccgaacca cagcacctgc gtggccgcca
     4261 tgcccagctc gaagccgagc gcgctggtgc cggccaggtc ctgcgggagc cggttgagca
     4321 tcacgatccc gatcccgccc atggcaccca cggccgggag cagcagctgg tccccgcgat
     4381 ggccggacag gacgagcacc aggtgggcca cgacgaacag gccgacgtag acggcggcca
     4441 gctgcatcgg tccgggctcg aaggtgccgg tgacggcgac gttcgtcacc aggaacccga
     4501 gcgggacgag gaggaggatc ggaagcagca gccggagctc cgctccccgc caggcgatgc
     4561 gcatcaggcg gcgatcaccg gtccagtcgc atgcgcacgc gcccgactgt gatctcgtcg
     4621 ccgaagctca gcgcgacggg ccggtcgatg cggtgcccgt tgacgtaggt gccgttggta
     4681 ctgccctgat cctccacgaa ccagctgcgg ccacggaagg tcagcatggc gtgcgtggcg
     4741 ctggcgaagt cgtcctcgac gtagatcgtg ttgttgacgt tgcggccgat ggagttgatc
     4801 ggccccaggg cgatggtcga gccggccggc ggctcgtcct ccggcgactc gagcaccgag
     4861 agtcggccga tccccgagcg cgacgccatc tgcgcctcct ccgccgagcg caggtcgcgc
     4921 agcagc
//
LOCUS       DASTYP010000163         7220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1688378, whole genome shotgun sequence.
ACCESSION   DASTYP010000163 DASTYP010000000
VERSION     DASTYP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688378"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VFV59_07485"
     CDS             <1..673
                     /locus_tag="VFV59_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325709.1"
                     /translation="TEGGSLPAALRSDAPYAPSIDPASFTTEIDNPYFPLRPGTRWVL
                     TGSGDSEGEITTTTVLEETREVMGVTCVVVHDEVAVDGRPVEITSDWYAQDADGNVWY
                     FGEETAEYEDGKVTSTAGSWEAGVDGAQPGIIMPAHPDVGVTYRQEFYAGEAEDVAKV
                     VELGQSVSTPVGTYDDVLVTEDWTPLEPDVRERKFYARDVGLVREELVEGGSGETVLT
                     EFTSG"
     gene            complement(689..1369)
                     /locus_tag="VFV59_07490"
     CDS             complement(689..1369)
                     /locus_tag="VFV59_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325710.1"
                     /translation="MRSSARALPFVLVLMLLAACGSSSSAESLAATPGAPVSVASASA
                     APSPSASSSASGGVMPLAKGPLQPGRYTSTQFEPQLELTLGSGWAEQFPDDNDELALD
                     HDGGFLAITRVSSVVDPSGSGTTDAPDDLFAWLTAHGALTVEHTEDVAVAGLPGQMLE
                     ATVAHTAEMFAYPTGNMRVVEGDRVRYYVVPLEGPDLTIVVASQGTRFEDLIAAVEPV
                     VDSLEIVE"
     gene            1469..2968
                     /locus_tag="VFV59_07495"
     CDS             1469..2968
                     /locus_tag="VFV59_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5325711.1"
                     /translation="MPDDARSDPAATEQPGRLVRDLRGRIDRSAGGDAAAALRLELGR
                     ALLAQARVVEAVRELEALAVEPGCPSEIRAAALGSLAGALALSGRTAESEAIAGEVLD
                     SPDADAESRVLARSAIRGLRFFEGRFDEAVEHAIQIVRDARDAGPVARAEARLDMGGM
                     LYHADRFDEAEPWFALDADASEGQRQEAAGTLAQMNLVRGRWRDVLDALPAGGGTADP
                     DWSDLSHVVRPAIRAHALLHLDRAEVARRELGRPVVPGAPPISLVAAALLAELDGDLR
                     SVGALIERAVALGGDPRFRPQIRTWAVELVRLALAAGERPAAQRVVDTLEALAAMSTV
                     RSVAAASLAVRGMVERDPALLANAAELFAEGPRMVAAAQTLEELGVAHAQREERDPAV
                     AALQQALASWRALDAPADVRRVARRLADLGVRTRPVARPSESRTGWDALSPTEATVAM
                     LVGEGMTNAEIASRLVVSPRTVETHVAHVLAKLEVRTRAGIARHAAGRS"
     gene            complement(2982..3854)
                     /locus_tag="VFV59_07500"
     CDS             complement(2982..3854)
                     /locus_tag="VFV59_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325712.1"
                     /translation="MGESRDARLREATTIGFMGDELVAEIGDALLVLDPASGRVIDEV
                     AFPDLPRINGTLLTGDSLWLLDHDNGKVVRVDRESGEVEAEVEIGGRAVSLLATPDGI
                     WAGSAHILPESASLIDTDMNEIVRRLELGAFPVYDDGYLWFGRNETGWASTVRKVDPD
                     TGEIVASIDLDGAEGCYLGGRFPDVVWSWCFEPPPNDTDVTRLDVDALAVAAIQPLGA
                     GGGLLGVYEDTSWLGAEPADGSRRLLKVDNATNAVEAVYSSDHVMPITIHDGALWMID
                     TDAGALRRIDLADL"
     gene            complement(4049..4558)
                     /locus_tag="VFV59_07505"
     CDS             complement(4049..4558)
                     /locus_tag="VFV59_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325713.1"
                     /translation="MRRPLTVLATAGLLAAFARPVAAASPSRTVQVIAPYSLEDALAD
                     YAASGDAFASCGEFDILATFEGTVTVTDWGDRMLRHVVFTGRFYNALDPSRSAGRIGN
                     TATWRLFDAAGNWTEVHIHGISNMAVLDDGGRIAIDVGYSAFSLALEQELASAGPEEN
                     SAALCEALD"
     gene            complement(4657..5844)
                     /locus_tag="VFV59_07510"
     CDS             complement(4657..5844)
                     /locus_tag="VFV59_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325714.1"
                     /translation="MKPERNLDRALEEFMDAGPESIPRPVLEDALEQVERTVQRPGVV
                     AGVETSQLWLALPAVAAAAVLIAAFIVGPWAGPRVASPDASTSPTASPEASPTLPAAT
                     VVVPDAVIDVPGAFLTLADGEDRVWLTSDEALVAIDTTTGATTSFAVPVPAGSWAGLQ
                     LLDGSLWIGNYDEGVVYRVSRETGEIEAEIPVGSEAASLTAAGGGMWVRTRNALNWEA
                     QRIDPATNRVVTTVEGGNAMAQGHGSLWFSQRGTNRIIRADPVTGEATAVIEVPPDHD
                     CGISSSAEAVWASCLSPDRVIGSVVRIDPATDEAAVVHTGAAGGGAFDAGGRTWLAAT
                     SPDGGVFMSVNLSSNSVEQILLVGPDFNPDNVVIAGDSAWVANDSADEVFRFAQDVFA
                     GGG"
     gene            complement(5841..6386)
                     /locus_tag="VFV59_07515"
     CDS             complement(5841..6386)
                     /locus_tag="VFV59_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5325715.1"
                     /translation="MDAQLVELAQRGDRSAFGQLATEFGDRLYALAYRMLRDSDLAAD
                     AAQESIVLIWRDLRALRDPRRFETWAYRILVRACYREVRRQRRPVPRLYALHELSAGD
                     SADAVADRDELDRAFARLTADQRAVLTLQYYLGMTHPQIADVLEIPVGTVGSRLHAAK
                     RALRGSLDAEARTASVGGRVG"
     gene            6836..7060
                     /locus_tag="VFV59_07520"
     CDS             6836..7060
                     /locus_tag="VFV59_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325716.1"
                     /translation="MDDRLLLAQLVTLAGLIGATIEYIEVVRRRTARRQLAAQRAALE
                     EDGLRGLVRVFLADGSDGRLRPSPRRGDPA"
BASE COUNT         1073 a         2487 c         2599 g         1061 t
ORIGIN      
        1 gaccgagggc ggctcgctgc ccgccgccct ccggtccgat gctccctacg ccccttccat
       61 cgaccctgcg tcgttcacga ccgagatcga caacccgtac ttcccgctgc ggccgggcac
      121 gcgctgggtg ctcaccggct cgggcgacag cgagggtgag atcacgacca cgacggtgct
      181 cgaggagaca cgcgaggtga tgggcgtcac ctgcgtggtg gtgcacgacg aggtcgcggt
      241 cgacggcagg ccggtggaga tcaccagcga ctggtacgcc caggacgccg atggcaacgt
      301 ctggtacttc ggcgaggaga cggccgagta cgaggacggc aaggtgacca gcaccgccgg
      361 gtcctgggag gcgggcgtcg atggcgcgca gccgggaatc atcatgccgg cccatccgga
      421 cgtcggcgtc acgtaccgcc aggagttcta cgccggcgag gcggaggacg tggcgaaggt
      481 ggtcgagctc gggcagtcgg tcagcacgcc cgtcgggacg tacgacgacg tgctggtcac
      541 cgaggactgg acgccgctcg agccggacgt gcgggagcgc aagttctacg cgcgcgacgt
      601 cggcctggtg cgggaggagc tggtcgaggg tggcagcggc gagaccgtgc tgacggagtt
      661 cacgagcggc tgaccgcgca gcggtccgct actcgacgat ctccagcgag tcgaccaccg
      721 gctcgacggc cgcgatgaga tcctcgaacc gggtgccctg cgaggcgacg acgatggtca
      781 ggtccggccc ctcgagcggg acgacgtagt accgcacgcg gtccccttcc acgacgcgca
      841 tgttgcccgt ggggtacgcg aacatctcag cggtatgcgc gaccgttgcc tcgagcatct
      901 ggcccggcag tccggcgacg gccacgtcct cggtgtgttc gacggtgagg gcgccatggg
      961 cggtcagcca ggcgaagagg tcgtcgggcg catcggtcgt tcctgacccc gacggatcga
     1021 cgacgctgct gacgcgcgtg atggccagga agccaccgtc gtggtcgagg gcgagctcgt
     1081 cgttgtcatc ggggaactgc tcggcccagc ccgatccgag cgtgagctcg agctgcggct
     1141 cgaactgcgt cgacgtgtag cggccgggtt gcagcggtcc cttggccagc ggcatcacgc
     1201 cgcctgaggc agacgacgaa gccgatggcg acggcgccgc cgaggccgat gcgacggaga
     1261 ccggcgcgcc gggagtggcc gccagcgatt cggcgctgga actggagccg cacgccgcga
     1321 gcagcatgag gacgaggacg aacgggaggg ctcgggcgga gctgcgcatg cgcgaacggt
     1381 ggcgtccacc ggctgcgccc acatccgcag aactactgaa ctttcgttgc ggggcgcggg
     1441 cggctcgcga ctatgctgcc tcgagaccgt gcctgacgat gctcgctccg acccggcggc
     1501 gaccgagcag cccgggagac ttgtccgcga cctgcgcggg aggatcgacc gttcggctgg
     1561 cggcgatgct gcggcggcgc tgcggctcga gctcggccgg gccctgctcg cccaggcgcg
     1621 cgtcgtcgag gccgtgcgag agctggaggc gctcgccgtc gagccgggct gcccatccga
     1681 gattcgggcc gcggccctcg gctcgctggc cggtgcgctg gcgctcagcg gccggacggc
     1741 cgagagcgag gcgatcgccg gcgaggtgct ggactcgccc gacgccgatg ccgaatcgcg
     1801 ggtcctggca cgctcggcca tccgcgggct gcgattcttc gagggccggt tcgacgaggc
     1861 ggtcgagcac gcgatccaga tcgttcgcga cgctcgcgac gcgggcccgg tcgcgcgagc
     1921 cgaggcccgc ctggacatgg gcggcatgct gtaccacgcc gatcggttcg acgaggcgga
     1981 gccgtggttc gcactggacg ccgacgccag cgagggtcag cgccaggagg cggcggggac
     2041 gctggcgcag atgaacctcg tccgaggccg atggcgggat gtgctcgacg cgcttccggc
     2101 tggcgggggg acggccgatc cggactggtc cgacctttcg catgtcgttc gtccggccat
     2161 ccgcgcccat gcgctgctcc atctcgatcg tgcggaggtg gccaggcggg agctcgggcg
     2221 tccggtcgtg cccggagcgc cgccgatttc cctggtcgca gccgccctgc tggcggagct
     2281 ggacggcgac ctgcgctcgg tgggcgcact gatcgagcgc gccgtggccc tcggtggcga
     2341 tccccggttc cgcccgcaga tccggacctg ggccgtcgag ctggttcgcc tggcactcgc
     2401 cgccggcgaa cgtccggccg cgcagcgcgt ggttgacacg ctggaggcgc tggcagcgat
     2461 gagcaccgtg cgctcagtgg cagcggcatc cctggcggtg cgcggaatgg tggagcgtga
     2521 ccccgcgctg ctcgcgaacg ccgccgagct ctttgccgag ggcccgcgca tggtcgcggc
     2581 cgcgcagacg ctcgaggagc tcggtgtcgc gcacgcccag cgcgaggagc gcgatcccgc
     2641 ggtggcggcg ttgcagcagg ccctggccag ctggcgggcg ctggacgcgc ccgcggacgt
     2701 ccggcgcgtt gcgcgacggc tcgcggacct cggcgtgcgc acgcgcccgg tggcgcggcc
     2761 gagcgaatct cgcacgggat gggacgcgct gtcgccgacc gaggcgaccg tggccatgct
     2821 ggtcggcgaa ggaatgacga acgccgagat cgcgagccgg ctcgtcgtgt cgccgcgcac
     2881 ggtcgagacg cacgttgcgc atgtgctcgc caagctcgag gtgaggacgc gcgccgggat
     2941 cgcgcgacac gcggccggac gaagctgacc gcccgccgcc cctagaggtc cgctaagtcg
     3001 atccgccgca gggcgccggc atccgtgtcg atcatccaca gggcgccgtc gtggatcgtg
     3061 atcggcatca catggtcgga tgagtacacg gcctcgaccg cgttcgtggc gttgtcgacc
     3121 ttcagcaggc gccggctgcc atcggccggc tccgctccta accacgaggt gtcctcgtaa
     3181 acaccgagca gtccgccgcc cgcgcccaga ggctgaatcg cggcgacggc gagcgcgtcc
     3241 acgtcgagcc gagtgacgtc ggtgtcgttc ggcggcggct cgaagcacca ggaccacacg
     3301 acgtccggga accggccacc caggtagcag ccctcggcac cgtcgaggtc gatggatgcg
     3361 acgatttcgc cggtgtcggg atccactttc cgaacggtgg atgcccaccc cgtctcgttg
     3421 cggccgaacc acaggtaccc gtcgtcatat accgggaagg cgccgagctc gagccgacgg
     3481 acgatctcgt tcatatcggt gtcgatcagc gaggccgatt cgggaaggat gtgcgcggat
     3541 ccggcccaga tgccgtccgg ggtcgcgagc aggctgaccg cacgtcctcc gatctcgacc
     3601 tccgcctcca cttcgccaga ctcacgatcg acccgaacga ccttgccgtt gtcgtgatcg
     3661 aggagccaga ggctgtcgcc cgtcaggagc gtgccgttga tccgcggcag atcagggaag
     3721 gcgacctcgt cgatcaccct cccggatgct gggtcgagga ccaggagggc atctccgatc
     3781 tcggccacga gctcgtcccc catgaagccg atggtcgtcg cttcgcgcag ccgcgcgtcg
     3841 cgcgactccc ccaccagtgc gccggccggc agttgggttg cgccgggctc ctgcgcggac
     3901 gctgaaggcg cccgggaggg atcaccggcc gacggcgccg cctgcggcgt tggcgtactc
     3961 gccagtgggg ccgtcgaccc accgcacccg gccagggcca gggcgacggc cgccgcaagc
     4021 ggaacgggag acagccgtga ccgccgcatc agtcgagggc ctcgcacagg gccgcactgt
     4081 tctcctcggg tccggcagac gcgagttcct gctcgagggc aaggctgaag gcgctgtacc
     4141 cgacgtcgat cgcgatccgg cctccgtcgt ccaggacagc catgttcgag atgccgtgga
     4201 tgtggacctc ggtccagttg cccgccgcgt cgaagagccg ccaggtggcg gtgttcccga
     4261 tgcgtccggc cgagcgcgag ggatccaagg cgttgtagaa gcgccccgtg aagaccacgt
     4321 gccgcagcat gcggtcgccc cagtccgtga ccgtgacggt gccctcgaac gtggcgagga
     4381 tgtcgaactc cccgcagctc gcgaaggcgt cgcccgaggc cgcgtagtcg gcgagggcgt
     4441 cttcgagcga gtacggggcg atgacctgga ccgtccgtga cggcgatgcg gccgcgaccg
     4501 gtctcgcaaa ggcggcgagg agcccggctg tcgccaggac ggtgagtggg cgtcgcatgg
     4561 tcggtctctc cgcttcgtgc ccccggcgca tccggcgggt catggcagaa agggcgttca
     4621 cgggcgcgaa cccttcagat ttcgctcgtg accagatcac ccgccgccgg caaagacgtc
     4681 ctgcgcgaag cggaacacct cgtcggccga gtcgttcgca acccacgccg aatccccggc
     4741 gatcaccacg ttgtccgggt tgaagtccgg gccgaccagc aggatctgct cgacgctgtt
     4801 cgagctgagg ttgaccgaca tgaacactcc gccgtcagga gaggtcgccg cgagccaggt
     4861 acgcccgcca gcgtcgaacg cgccgccacc ggccgcgccg gtgtgcacga cggcggcctc
     4921 gtcggtggct gggtcaatcc tcacgaccga gccgatgact cggtcgggag aaaggcagct
     4981 ggcccacacg gcttccgccg agctgctgat gccgcagtcg tgatccggcg ggacctcgat
     5041 caccgctgtc gcttctccgg tcaccgggtc ggctcggatg attcgattcg tgcctcgctg
     5101 tgagaaccag agcgatccgt ggccttgcgc catcgcgttc ccgccctcga cggtcgtcac
     5161 cactcggttc gtcgcggggt cgatgcgctg cgcctcccag ttcagggcgt tccgggttcg
     5221 aacccacatg ccgccgcccg cggccgtcag gctggccgct tcgctcccga cgggaatctc
     5281 agcctcgatc tcgccggtct cacgactgac gcggtacacg acgccctcgt cgtagttgcc
     5341 gatccacagg ctgccatcga ggagctgcag acctgcccaa gagcctgccg ggactgggac
     5401 cgcgaagctc gtggtcgcgc cggtggtcgt gtcgatggcg acgagcgcct cgtcggacgt
     5461 caaccacacg cggtcctcgc catccgcaag ggtgaggaac gcgccgggca cgtcaatgac
     5521 cgcgtccggc acgacgaccg tcgccgccgg aagggttggc gaggcttcgg gtgacgccgt
     5581 cggcgacgtt gacgcgtccg gcgacgcgac gcgtggccct gcccatgggc caacgatgaa
     5641 ggcggcgatc aggacagcag cagcagcgac cgccggcaat gcaagccaca gctgacttgt
     5701 ctcgacacct gccaccactc ctgggcgctg cacggttcgc tcaacctgtt cgagcgcgtc
     5761 ctccagcacc gggcgcggaa tcgattccgg acccgcatcc atgaactctt cgagggcgcg
     5821 atcgagattg cgctccggtt tcatcccacg cgacctccga cggaagcggt gcgcgcctcg
     5881 gcgtcgagcg acccgcggag cgcgcgcttc gcggcatgaa gccgcgagcc gaccgtgccg
     5941 accgggatct cgaggacgtc ggcgatctgc gggtgcgtca tcccgaggta gtactggagg
     6001 gtgaggacgg ctcgctggtc agcggtcagg cgcgcgaacg cacggtcgag ctcgtcacgg
     6061 tccgccaccg catccgcgga gtcgcccgcc gacagttcgt gcagggcgta cagccgcggg
     6121 accggccggc gttggcggcg cacctcgcgg tagcaggcgc ggaccaggat gcggtatgcc
     6181 caggtttcga accgccgcgg gtcccgtagg gcgcgcaagt cgcgccagat gagaacaatc
     6241 gactcctgcg ctgcatcggc cgcaaggtcg ctgtcgcgca gcatccggta ggcgagggcg
     6301 tacagccggt cgccgaactc cgtcgccagc tgaccgaatg cgctcctgtc gcctcgttgt
     6361 gccagttcca ccagctgcgc gtccatcgcc tccactcatg tgacgacggc gcaaagcgtc
     6421 gcccatcggt gcatagggcg ccagcggcag tgaacccttc agttttcgcg acgcgtgaat
     6481 ggctgcgggt cgcagcctgc aacgccggag ggtggacgag gaggggcacc ggcaccgccg
     6541 gccgggggga gtgaccggca gccccgatgc cccgccccag cgtaccgccg ttccgctcaa
     6601 cgtggcctca atttgtcccg acctggatgg cgcacgtgag gaccggttca gtccctcgcg
     6661 gagaagagac ggaccgccgg gctgcctacc caacccgata cgggcgggaa ccggcgatcc
     6721 gtcccgcctg tgcaaacgca gcatcccgtg cgacgttagg acgcgtgccg gcccagatgg
     6781 gctagccgta tcgtctgctt cagctgggcg gcgttgaagc gtcacgatgg tcgacatgga
     6841 cgaccggttg ttgctcgcgc agctggtgac gctggcgggg ctgatcggag cgaccatcga
     6901 gtacatcgag gtggtgcgtc gccgcacagc gcgccgtcag ctcgccgctc agcgcgcggc
     6961 gttggaggaa gacgggctgc gcggcctcgt gcgcgtattc ctcgcggacg gttctgacgg
     7021 ccgacttcga ccttcgccac gtcgcggaga ccctgcctga ccaggcggag accgctgtcg
     7081 acctcagtct tcgctcaggc ccagctccac cgcagcgtcg atgtcaagcg ctcgtccgcg
     7141 ctccatcgcg tcctcgagtt ccgcggacgt cagctggttc tccaggagtc gcagcatctc
     7201 gtcacgccgt cgctgctggt
//
LOCUS       DASTYP010000164        12978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_450172, whole genome shotgun sequence.
ACCESSION   DASTYP010000164 DASTYP010000000
VERSION     DASTYP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450172"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /gene="tatA"
                     /locus_tag="VFV59_07525"
     CDS             complement(<1..134)
                     /gene="tatA"
                     /locus_tag="VFV59_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5325717.1"
                     /translation="MPVPGPWELILLLVIVLIIFGPGKLPDIGNAVGKGIREFRKAST
                     "
     gene            complement(139..1002)
                     /gene="pyrF"
                     /locus_tag="VFV59_07530"
     CDS             complement(139..1002)
                     /gene="pyrF"
                     /locus_tag="VFV59_07530"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU5325718.1"
                     /translation="MTSTPLDRLRRRVGALGAPLCLGLDPHPDALPEGLSPDVHGVEA
                     FARGLLEAAADQVVAVKFNVAFFEAFGSAGLAALERVRRDVPAALLCILDAKRGDIGS
                     TAERYAEALFGHLDADAVTLSPYLGEDAIEPFLRHPDRLVYVLARTSNPSAGQVQDLE
                     VAPREPLALHVARWVAERWADGRVGLVVGATAAEELRAIRDAVPGPGFLVPGVGAQGG
                     DLEASVACAAGAWAPGVVSVSRGIALASRGDDWRAAARQAAGVLRSRMAEAVLHSNSS
                     ADPAPHSTGGS"
     gene            complement(999..2744)
                     /gene="recJ"
                     /locus_tag="VFV59_07535"
     CDS             complement(999..2744)
                     /gene="recJ"
                     /locus_tag="VFV59_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU5325719.1"
                     /translation="MIAPSLEWLLPEPVAEPPSFTGFGRPVATLLARRGFRDDAQLRA
                     FLDAGAGALHDLSRMADADQALDRIDAALERGERIAIWGDYDADGMTAIVVWVLALRR
                     LGADPVRYVPSRLSEGYGLSAAGLRDLAGRGVGLVITCDCGVSNAAEVEVARQLGMDV
                     VVTDHHLPPAELPRAVAVVDPHRPDCAYPDPDLTGAGLSYKLGAALLARHGRTAEGLA
                     AVAALGTIADLAPMTGESRAIVRLGIEELARTDHAGLRALLARACEAPGQPTARDLAF
                     GVVPRINAAGRIADAELALQLLLTEDPVRAGALAEELEAVHVRRRELTREAVEAARSL
                     VSADGTDAPLVLREDGWAPGLLGLIAGRLADSLARPVAIATLVGDEVRGSVRAPSDFH
                     VASALEACGDLLLKRGGHAAAGGFSAHPDRWDAFIAAFGALGRPFPADGAAETQRPGR
                     QPVDLVITARHLDWTLADQLERLAPYGPGHVEPVLAVTGMTVREARRIGAREAHVAFR
                     MRRGLETFDAVAFGLDADRALPQPDDELDLVGTLERDAFGGLPRLRLRVLDFAHAAAS
                     PLLARRLPPPVAAVG"
     gene            complement(2814..3956)
                     /gene="secF"
                     /locus_tag="VFV59_07540"
     CDS             complement(2814..3956)
                     /gene="secF"
                     /locus_tag="VFV59_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU5325720.1"
                     /translation="MYDIVGKRNLWFAISAILTIPGLIFIFLGGLKPSIDFTGGTEWE
                     VRYADQPSAAEMTSALTELGYEEVLVRQLEDGFLRIRTEPIDLIPAETAAPSPSASVS
                     PSGSGSVAPSASASPSGSGSASASPSASASPSASAAASASPSAGAASGSAAPSPSASP
                     APLAEGTEFANLEQQLESRFGAGDPRSVRTVGPIIGADLIRSSAILIIIGELFILAYL
                     WVRFGFRFGTAAIVALLHDVILVVGTFAILGFFLNVEFDALFVTALLTIIGFSVHDTI
                     VVFDRIRENRVRHAGESFGAIVNHSILQTLGRSIITSLTVVVTLGALLLLGPPTIRTF
                     TLALLLGVVSGTYSSIFNASQILVAWHEWDAKRKARDAGRPARRPA"
     gene            complement(3960..5294)
                     /gene="secD"
                     /locus_tag="VFV59_07545"
     CDS             complement(3960..5294)
                     /gene="secD"
                     /locus_tag="VFV59_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU5325721.1"
                     /translation="MTWRRIAPWVIGVVAILAIYIDLPRKTLGIENLLPASIGGMEFK
                     TVLGLDLEGGLRVTLEAVQTGDQPITPEDMETARGIIERRVAGIGVSEPQVRTEVGGD
                     GSQRIVVEVPGVSDQNQVRDLVGSTGQLQFIDPQGQQLTDGQDISQLLQAGTVRELFN
                     GGEIEPGSVAPAVQGNQIGVQFSLKPTGSDIWCQFTTANVNRPGPIALDAEIITTPNI
                     NSAICGGQTIITVGANTPANQQEQTNLYNTLRFGALPIALTEQGVETVDPTLGAGFLV
                     QALVAGAVGLLLVLFFMITYYRLPGVLAALALVFYTLVVYAVFRVIHVTLTLAGVAAF
                     ILSIGMAVDANILIFERMKEEIRAGKTLGPAIEAGFNRAWSSILDSNVSSLLVAAWLY
                     WQGTTVVRGFALVLIVGVLVSMFSAVTVTRTMLRYVTRTSWGRRLELYHVER"
     gene            complement(5417..7417)
                     /gene="uvrC"
                     /locus_tag="VFV59_07550"
     CDS             complement(5417..7417)
                     /gene="uvrC"
                     /locus_tag="VFV59_07550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5325722.1"
                     /translation="MSPARPLPDTLRAVLRRLPTSPGVYLMKNADERVLYVGKADSLR
                     NRVRSYFAARGPEDARIARMVTEVADVEYIVTDTVSEAYLLESNLIKEHRPRFNIRLR
                     DDKSYPFVKITMGEDFPRIVRTRKLARDGSRYFGPYASASSVDETLKLLRKIFPFRTC
                     NLDIPEGKRVLERPCLLYYINRCQGPCIQAIEKAEYRATIGQIVDFLEGKQEPIAAEL
                     RREMESHSDALRFEQAAASRDKLRAVERTMEQQKMAAFSRAEHDVIGLAREEDEACVQ
                     VMQVRNGKLIGREHFIVEGARDATDAEVLGGFLQQYYANTDALPRALLAPLMPAGADD
                     LAAYLADRRGVRVTITVPERGEKRRLVALATQNAVEALERERAEWLADAAKRDEALTE
                     LADALGLPKLPERIECYDMSNIQGTSAVGSMVVFIDGRPEPREYRRFRIRSGDTPDDF
                     RMMAEVLRRRFSRVAKLRADTGALSLAAVGADEAPEGDTDLPADLDPDAGPVDGGAHG
                     NGDQPREAAWSVPDLVIVDGGKGQLSAAVGALTELGITDVPLTGLAKRFEELYLPDRP
                     DPIVLPRRSQALYLVQRIRDEAHRFAITYHRDVRGRRALRSELDDIAGIGPGRKKALL
                     KRFGSVRRIREASVEEVAGTPGISRDLAERVKGHLAREGMLA"
     gene            complement(7428..7943)
                     /locus_tag="VFV59_07555"
     CDS             complement(7428..7943)
                     /locus_tag="VFV59_07555"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5325723.1"
                     /translation="MSSPLRRSRRLRWTDLPRLLVGGAVQFAALFGGRYRRTEGAHVL
                     CLDREGRILVVRTTYLGPGWMLPGGRIERHETPHDGAARETREETGLEVEVDRLLLVE
                     ARRGRDVSFVFGGRVIGGELEPQLGEIAEVGWLTRGEIAATSPRLHRLLTLLDDGGSP
                     VVYRGLPRPRP"
     gene            complement(7921..9057)
                     /locus_tag="VFV59_07560"
     CDS             complement(7921..9057)
                     /locus_tag="VFV59_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU5325724.1"
                     /translation="MRAQATPDQPVPYDRELIRSGGVSSSERRQARPSGDGGRRPPRQ
                     PRRRRGGFGRVLGTVLVGLLLVVVVVGVVLFGRVAAFNASVSTAPFPSTALFGALNGS
                     DRVNVLLVGYGGADHDGAYLADSIQVLSIDPTTDTTTTIPIPRDLWIEGVGAYPRNGK
                     VNEVFSTGHANGGLEEAGALLADVLSDVTGLRIEHWLSIDFAGFRDMIDAVGGVTIEN
                     PTEFSYTNNETDYANRNFNLGTFPAGELRLDGAQALAYARARYTDVPSESNDFARSVR
                     QARILGALRDELGDGGIGSIVPGLRLMDAMEGRVRTDLSAIDLALLASHLHSDRRIEL
                     SEDVVLRATTNTVGQYILIPVDWTGPGDYAVIRSYLADELSAAP"
     gene            9174..10844
                     /locus_tag="VFV59_07565"
     CDS             9174..10844
                     /locus_tag="VFV59_07565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEU5325725.1"
                     /translation="MDGSAAVTTGPGLDAILGALDPEQRAAATLPDGPAQIIAPAGSG
                     KTTTMVARLAVLLARGVAPERICVVTFNRDAAQDLKARVERRLASVVPRAREIEIRTL
                     HALARQVALDAGERREIVADRLPLLRAARRRVAREDEPPPPEAADLDTWLSAWRIEGR
                     PPPEQARATIAAYEDLLRARGLLDFDDLVVGAADRLERDPRLRLRWQARFAHVCVDEF
                     QDVDAAQLRLVRLLAAPEDNLFVVGDDDQTIYAWRLADVRRILRFSADYPDARRVMLA
                     TNYRCPTAVVAASARLVAVNRERFAKPIRAAPDLADDPRAIEAWPSGDAEWPDHAARL
                     AADATRRGRQICFLARTRAELVPVFLALVRAGVPHRPNVPPIVEAAPVTTLVDAARAT
                     PPSAPPFRALLGLRAGHGWRRGDPGADHLSDEDQAALDALLGWSVGFRRLGAFVGAFD
                     AARARIHALQSDDAVVELATVHASKGREWETVVLVGFEEGRIPNRRSIEDADDSERAV
                     EEERRLAYVALTRATRHLVLAFDPARPSPFLAEMGFALRPASASRRLS"
     gene            10943..11161
                     /locus_tag="VFV59_07570"
     CDS             10943..11161
                     /locus_tag="VFV59_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325726.1"
                     /translation="MRQRAARAAIVSLFVVGGLLGLAAAASAQSPDVLPVEPDGGIGG
                     EVVLPVEPDGGIGGEVVLPVEPDGGIGN"
     gene            complement(11257..>12978)
                     /gene="uvrA"
                     /locus_tag="VFV59_07575"
     CDS             complement(11257..>12978)
                     /gene="uvrA"
                     /locus_tag="VFV59_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5325727.1"
                     /translation="AMRAEIERYMTTKPCPTCKGMRLKPESLAVTVAGRNIIETTQRS
                     VTDALAWYDALPKRLNDRENQIARQVLKEIRARLGFLVDVGLDYLTLDRASGTLSGGE
                     AQRIRLATQIGSSLMGVLYILDEPSIGLHQRDNDRLIATLVRLRDLGNTLLVVEHDED
                     TIRAADWVIDIGPRAGEHGGELIYSGDLAGLLAEPRSITGAYLRGERHIPVPKRRRNG
                     KRDALVVRGARENNLQAIDVAFPLGRFVAVTGVSGSGKSSLVTQILYPALAQRIWGAR
                     EPAGRHDRIEGIEHIDKVIEIDQSPIGRTPRSNPATYTGLWTPLRELLAAVPEARLRG
                     YKPGRFSFNVKGGRCEACEGAGIVQIEMHFLPDVYVACEVCKGKRYNRETLEIHYKGK
                     NVAEILEMTVEEALDFFSAVPSIRNKLQTLVDVGMGYIHLGQPATTLSGGEAQRVKLA
                     TELSKRATGKTFYILDEPTTGLHFDDVARLLSVLGRLVEAGNTVVVIEHNLDVIKTAD
                     WVIDLGPEGGDKGGRLVASGTPEQVARAAGSYTGKYLRPVLGIADGDAASPSRPKSHR
                     RVSASVS"
BASE COUNT         1826 a         4663 c         4672 g         1817 t
ORIGIN      
        1 tcggtggagg ccttgcggaa ctcgcgaatc cccttcccga cggcgttgcc gatgtccggc
       61 agcttgccgg ggccgaagat gatcaggacg atcaccagca gcaggatgag ctcccatggt
      121 ccgggaaccg gcatgaactc acgaacctcc ggtggaatgc ggcgccggat cggcgctcga
      181 attcgagtgt agcaccgcct cggccatccg gctccgcagg accccggctg cctgacgcgc
      241 cgccgcgcgc cagtcgtcgc cgcgcgaggc aagcgcgatc ccccgggaca cgctgacgac
      301 gcccggggcc cacgcgcccg cggcacacgc cacggacgcc tccaggtcgc caccctgggc
      361 tccgacgccc gggaccagga accccggccc gggcaccgcg tcgcggatcg cgcgcagctc
      421 ctcggccgcg gtcgcgccga ccacgaggcc cacccgccca tccgcccagc gctcggcgac
      481 ccagcgcgcc acgtgcagcg cgagcggctc gcggggtgcg acctcgagat cttgcacctg
      541 gccggcggag ggattgctgg tccgcgcgag gacgtacacc aaccggtcgg ggtggcgaag
      601 gaacggctcg atggcgtcct cgccgagata cggcgagagc gtgaccgcgt ccgcgtcgag
      661 gtggccgaac agcgcctcgg cgtagcgctc cgccgtggag ccgatgtcgc cgcgcttggc
      721 gtccaggatg cagaggagcg ccgccgggac gtcccgccga acccgctcga gggcggccag
      781 gccggccgac ccgaatgcct cgaagaacgc cacgttgaac ttcaccgcga cgacctggtc
      841 agccgcggcc tcgaggaggc cacgcgcgaa cgcctcgacg ccgtggacgt cgggcgagag
      901 gccctcgggc agggcgtccg ggtgcggatc gaggcccagg cacagtggcg cgcccagcgc
      961 gccgacgcgc cgccgcagcc gatcgagggg agtggacgtc atccgacggc ggcgaccggc
     1021 ggcggcagcc gccgggccag gagcgggctg gcggccgcgt gcgcgaagtc caggacgcgg
     1081 aggcgcagcc gcggcaggcc cccgaacgcg tcgcgctcca gcgttcccac caggtcgagc
     1141 tcgtcatcgg gctggggcag cgcacggtcg gcgtcgagcc cgaacgcaac ggcgtcgaag
     1201 gtctcgaggc cgcggcgcat gcggaaggcg acgtgcgcct cccgcgcgcc gatgcgtcgc
     1261 gcctcccgca ccgtcatgcc ggtcacggcc agcaccggct ccacgtggcc ggggccgtac
     1321 ggtgccaggc gctcgagctg gtccgcgagg gtccagtcca ggtgccgcgc ggtgatcacg
     1381 agatcgacgg gctgccgccc cggtcgctgg gtctcggccg ccccgtccgc cgggaacggg
     1441 cggccgaggg caccgaacgc ggcgatgaag gcatcccacc ggtccggatg ggcgctgaag
     1501 ccgcccgccg cggcatgccc gccgcgcttc agcagcaggt cgccgcaggc ctccagggcc
     1561 gaggcgacgt ggaagtcgga gggggcgcgg accgagccgc ggacctcgtc cccgaccagc
     1621 gtcgcgatgg ccaccggccg cgccagcgaa tcggccaggc gtcccgcgat cagccccagc
     1681 aggccgggcg cccacccgtc ctcgcgcagg accagcgggg cgtcggtgcc gtcggcggac
     1741 accaggctgc gcgccgcctc gaccgcttcg cgggtcagct cgcgacggcg cacgtgcacc
     1801 gcctccagct cctcggcgag cgccccggcg cggacgggat cctcggtcag caggagctgg
     1861 agcgccagct ccgcgtcagc gatgcgtccg gcggcattga tgcgcggcac gacgccgaag
     1921 gccaggtccc gagccgtcgg ctgaccgggc gcctcgcagg cgcgcgccag gagcgcgcgg
     1981 aggcccgcgt gatccgttcg cgcgagctcc tcgatcccca gccgcacgat ggcgcggctc
     2041 tcccccgtca tcggggccag gtccgcgatg gtcccgaggg ccgccacggc ggcgagcccc
     2101 tcggccgtcc tgccgtgccg ggccagcaag gcggccccga gcttgtagga cagcccggca
     2161 ccggtcaggt cgggatcggg gtacgcgcag tcgggccgat gcgggtcgac caccgcgacc
     2221 gcgcgcggca gctccgcggg gggcaggtgg tgatcggtca ccaccacgtc catgccgagc
     2281 tgccgggcca cctccacctc ggccgcgttg ctcacgccac agtcgcaggt gatcaccagc
     2341 ccgacgccgc gtccggcaag gtcgcgcagg ccggccgccg acagcccgta tccttcggag
     2401 agccgcgagg ggacgtagcg aacggggtcg gcgccgaggc gccgcagcgc cagcacccag
     2461 accacgatcg cggtcatgcc gtccgcatcg tagtcgcccc agatcgcgat ccgctcgccc
     2521 cgctccagcg ccgcgtcgat ccggtcgagc gcctggtccg catcggccat ccgcgagagg
     2581 tcgtgcaggg cgcccgcgcc ggcatcgagg aaggcacgga gctgcgcatc gtcgcggaag
     2641 ccgcgtcggg ccaggagcgt cgccaccggc cggccgaagc cggtgaagga cggtggctcg
     2701 gcaaccggct cgggcaggag ccactcgagg ctcggggcga tcatcggcgc catggtagcg
     2761 gcggccgatg acccggcgct taccgttcgg ttaccggggc tggcgggcgc cggtcaggcg
     2821 gggcgtcgcg cggggcgacc ggcgtcgcgc gccttgcgct tcgcgtccca ctcgtgccac
     2881 gccaccagga tctggctggc gttgaagatc gacgagtagg tgccgctcac gacgcccagc
     2941 agcagcgcca gcgtgaaggt ccggatcgtc ggcgggccga gcagcagcag cgcgccgagg
     3001 gtcaccacca cggtcagcga cgtgatgatc gagcggccca gcgtctggag gatcgagtgg
     3061 ttgacgatgg cgccgaacga ctcgcccgcg tgccggaccc ggttctcgcg gatgcggtcg
     3121 aacaccacga tggtgtcgtg gacgctgaag ccgatgatcg tcagcagcgc ggtcacgaac
     3181 agggcgtcga actcgacgtt caggaagaag ccgaggatgg cgaatgtgcc gaccaccagg
     3241 atcacgtcgt gcaggagcgc cacgatcgcc gcggtcccga agcggaaccc gaaccggacc
     3301 cacaggtagg cgaggatgaa gagctcgccg atgatgatca ggatcgccga gctgcggatg
     3361 aggtcggcac cgatgatcgg gcccacggtc cgcacgctgc gcggatcccc cgccccgaac
     3421 cgcgactcca gctgctgctc gaggttcgcg aactcggtgc cctccgccag cggagccggg
     3481 ctggcgctgg gtgagggtgc cgcggaaccg gaggcggcac ctgcggatgg cgaggcgctg
     3541 gccgcggccg aggcgctggg agaggccgag gcgctgggcg aggcggacgc cgagcccgac
     3601 cccgatgggc tggcgctcgc cgatggcgcc accgatcccg agcccgacgg cgagaccgac
     3661 gcggacggcg acggggccgc cgtctcggcc gggatcaggt cgatcggctc cgtgcggatc
     3721 ctcaggaacc cgtcctccag ctgcctcacc aggacctctt cgtagccgag ctcggtcagg
     3781 gcgctggtca tctccgcggc cgacggctgg tcggcgtatc ggacctccca ctcggtgcca
     3841 ccggtgaagt cgatggacgg cttcaggccg ccgaggaaga tgaagatcag gccggggatc
     3901 gtgaggatgg cgctgatcgc gaaccacagg ttgcgcttgc cgacgatgtc gtacatgggc
     3961 tagcgctcca cgtggtagag ctcgagccga cggccccagc tcgtgcgggt gacgtatcgg
     4021 agcatggttc tcgtcaccgt gacggcgctg aacatgctca cgagcacgcc gacgatcagg
     4081 acgagggcga agccgcggac cacggtggtg ccctgccagt acagccaggc ggccaccagg
     4141 aggctcgaga cgttcgagtc gaggatgctg gaccaggccc ggttgaagcc cgcctcgatg
     4201 gcaggtccga gcgtcttgcc ggcgcggatc tcctccttca tacgctcgaa gatgaggatg
     4261 ttcgcgtcga ccgccatgcc gatgctgagg atgaacgccg ccacgccggc gagggtcagc
     4321 gtcacgtgga tgacgcggaa cacggcgtac accacgagcg tgtagaagac gagggccagg
     4381 gcggcgagca cgccgggcag ccggtagtac gtgatcatga agaagaggac gagcagcagg
     4441 cccacggcac cggccacgag cgcctgcacc aggaagccgg cgccgagggt cggatcgacc
     4501 gtctcgacgc cctgctcggt gagggcgatc ggcagcgcgc cgaaccgcag cgtgttgtag
     4561 aggttcgtct gctcctgctg attggcgggc gtgttggccc cgacggtgat gatcgtctgg
     4621 ccaccgcaga tcgcgctgtt gatgttcggt gtggtgatga tctccgcgtc cagggcgatc
     4681 ggcccggggc ggttgacgtt ggcagtcgtg aactggcacc agatgtcgct gccggtcggc
     4741 ttcagcgaga actgcacgcc gatctggttg ccctgcacgg ccggcgcgac gctgcccggc
     4801 tcgatctcac cgccgttgaa gagctcgcgg accgtgcccg cctggagcag ctggctgatg
     4861 tcctgcccgt cggtgagctg ctggccctgc ggatcgatga actgcagctg gccggtggag
     4921 ccgacgaggt cgcgcacctg gttctggtcg ctgacccccg gcacctccac cacgatgcgc
     4981 tgcgagccgt cgccgccgac ctcggtccgg acctggggct cgctgacccc gataccggcg
     5041 acgcgacgct cgatgatccc gcgcgcggtc tccatgtcct ccggcgtgat cggctgatcg
     5101 ccggtctgca ccgcctccag cgtcacgcgc agcccgccct cgaggtcgag cccgagcacc
     5161 gtcttgaatt ccatgccgcc gatcgaggca ggcagcaggt tctcgatgcc gagcgtcttg
     5221 cgcggcaggt cgatgtagat cgccaggatg gcgaccaccc cgatcaccca cggggcgatg
     5281 cgacgccagg tcatgctgtg gccgtcggtt cctcagtcac tcaggggatc cgtgccggcg
     5341 ccggagcgcc tggaacacga aacgcgccgc tgagcagcgg cgggcggctg cgagtatagc
     5401 ggctgcggtc ggggtgtcat gcgagcatcc cctcgcgggc gagatggccc ttcacgcgct
     5461 cggccaggtc gcggctgatc cccggcgtcc cggcgacctc ctcgaccgat gcctccctga
     5521 tgcggcggac cgacccgaac cgcttgagca gggccttctt gcgcccggga ccgatgccgg
     5581 cgatgtcgtc cagctcgctc cgcagcgcgc gacggccgcg gacgtcgcgg tggtacgtga
     5641 tggcgaaccg atgcgcctcg tcgcggatgc gctggacgag gtacagggcc tgcgaacgcc
     5701 gcggcaggac gatcgggtcg gggcgatccg ggaggtacag ctcctcgaac cgcttcgcca
     5761 ggccggtgag cggcacgtcc gtgatgccca gctccgtcag ggcgccgacg gcggccgaca
     5821 gctggccctt cccgccgtcg acgatcacca gatccggcac cgaccacgcg gcctcgcgcg
     5881 gctggtcgcc gttgccgtgc gcgccgccgt cgaccggccc cgcgtccggg tcgaggtcgg
     5941 cgggcaggtc ggtgtcgccc tcgggcgcct cgtcggcccc gacggcggcc agggacaggg
     6001 caccggtgtc cgcccgcagc ttcgcgacgc gactgaaccg ccgtcgcagg acctccgcca
     6061 tcatgcggaa gtcgtccggc gtgtcgccgg atcggatgcg gaagcgccgg tactcgcgcg
     6121 gctccggcct gccgtcgatg aagacgacca tgctgcccac ggccgacgtt ccctggatat
     6181 tgctcatgtc gtagcactcg atccgctccg ggagcttcgg cagcccgagg gcgtcggcca
     6241 gctcggtgag cgcctcgtcc cgcttcgcgg cgtcggccag ccactccgcg cgctcgcgct
     6301 ccagcgcctc gaccgcgttc tgggtggcga gggccaccag gcgccgcttc tccccccgct
     6361 ccggcacggt gatcgtgacg cgcacgcccc gccggtcggc caggtacgcc gccaggtcgt
     6421 cggcaccggc cggcatgagc ggggccagga gggcgcgggg cagcgcatcg gtgttggcgt
     6481 agtactgctg caggaagccg ccgaggacct ccgcgtcggt cgcgtcccgc gcaccctcga
     6541 cgatgaagtg ctcgcggccg atgagcttgc cgttgcgcac ctgcatcacc tgcacgcagg
     6601 cctcgtcctc ctcgcgtgcc aggccgatga cgtcgtgctc ggcgcggctg aaggccgcca
     6661 tcttctgctg ctccatggtc cgctcgacgg cccgcagctt gtctcgcgag gcggccgcct
     6721 gctcgaagcg cagcgcgtcg gagtgcgact ccatctcccg ccgcagctca gcggcgatcg
     6781 gttcctgctt gccctccagg aagtccacga tctggccgat ggtggcgcgg tactccgcct
     6841 tctcgatggc ctggatgcag gggccctggc atcggttgat gtagtacagc aggcacggcc
     6901 gctcgagcac ccgcttgcct tcgggaatgt ccaggttgca ggtgcggaac gggaagatct
     6961 tgcgcagcag cttcagggtc tcgtccacgc tggaggccga tgcatacggg ccgaagtagc
     7021 gactgccgtc cctcgcgagc ttcctcgtcc gcacgatgcg cgggaagtcc tcgcccatgg
     7081 tgatcttcac gaacgggtag ctcttgtcgt cccgcagccg gatgttgaag cgggggcggt
     7141 gctccttgat gaggttcgac tcgaggaggt acgcctccga gacggtgtcg gtgacgatgt
     7201 actcgacgtc tgcgacctcc gtcaccatcc gcgcgatccg cgcgtcctcg ggaccccgcg
     7261 ccgcgaagta ggagcgaacg cggttgcgca gcgagtcggc cttgccgacg tacagcacgc
     7321 gctcgtccgc gttcttcatc aggtacacgc ccgggctggt cggcaggcgg cgcagcaccg
     7381 cccgcagcgt gtcggggagc gggcgcgcgg ggctcatgac gtcgattcta gggtcgcggg
     7441 cgcggaaggc cccggtagac gaccggcgag ccgccgtcgt ccagcagcgt cagcagccga
     7501 tgcagccgcg gggaggtggc ggcgatctcg ccccgcgtca gccaccccac ctcggcgatc
     7561 tccccgagct gcggctcgag ctcgccgccg atcacgcgcc cgccgaacac gaagctcacg
     7621 tcacggcccc ggcgcgcctc cacgagcagc agccggtcga cctcgacctc caggcccgtc
     7681 tcctcgcgcg tctcgcgtgc cgcaccgtcg tgcggcgtct cgtgccgctc gatgcggccg
     7741 cctggcagca tccagcccgg acccaggtag gtggtccgca cgacgaggat ccggccctcg
     7801 cggtcgaggc acagcacgtg cgccccctcg gtgcggcggt agcggccgcc gaagagcgcg
     7861 gcgaactgga ccgccccacc gaccagcagc cgcggcaggt cggtccaccg cagccggcgg
     7921 ctacggcgca gcggagagct catcggccag gtagctgcgg atgacggcgt agtcgccggg
     7981 cccggtccag tccacgggga tgaggatgta ctgtccgacc gtgttcgtcg tcgcgcgcag
     8041 gacgacgtcc tcgctgagct cgatgcgccg gtcgctgtgg aggtgcgacg cgagcagcgc
     8101 caggtcgatg gccgagaggt cggtcctgac ccgtccctcc atggcatcca tcagccgcag
     8161 gcccggcacg atggagccga taccgccgtc ccccagctcg tcacgcagcg cccccaggat
     8221 gcgggcctgg cgcaccgatc gggcgaagtc gttggattcc gacggcacgt cggtgtaccg
     8281 cgcgcgggcg taggcgaggg cctgcgcgcc atccaggcgc agctcgcccg ccgggaacgt
     8341 cccgaggttg aagttgcgat tcgcgtagtc ggtctcgttg ttcgtgtagc tgaactcggt
     8401 cgggttctcg atcgtcacgc cgccgaccgc atcgatcatg tcacggaagc ccgcgaagtc
     8461 gatcgacagc cagtgctcga tgcgcaggcc ggtgacatcg gagagcacgt cggccagcag
     8521 ggcgccggcc tcctcgagcc ccccgttcgc gtggcccgtg gagaagacct cgttcacctt
     8581 gccgttgcgc ggatacgccc ccacgccctc gatccacagg tcgcgcggga tcgggatggt
     8641 ggtggtcgtg tccgtggtcg gatcgatgga gaggacctgg atggagtcgg ccaggtaggc
     8701 gccatcgtga tcggcgccgc cgtaaccgac cagcaggacg ttgacacggt cgctgccgtt
     8761 gagggcgccg aagagggccg tggacgggaa cggtgcggtc gagacgctgg cgttgaaggc
     8821 cgcgacgcgc ccgaacagga cgacgccgac gaccaccacg acgaggagca ggccgaccag
     8881 gacggtcccc aggactctcc cgaacccgcc ccggcgtcgg cgcggctggc gcggtgggcg
     8941 tcggcccccg tcaccggacg gtcgtgcctg ccggcgctcg gacgacgaca ccccgccgga
     9001 tcgaatcagc tcacggtcgt agggaacggg ttggtcgggc gttgcctggg ctctcacgcg
     9061 tgggacatgc tcgggtgcgg tggctccaac tcagggcggg agggagagga tagggcctgc
     9121 gtcggtaagc cgccggtcat ccggcggtaa gcggcgctcc gcatcatcgg cgcatggacg
     9181 gcagcgctgc ggtgaccacc gggcccgggc tggacgcaat cctcggtgcg ctggatcccg
     9241 agcagcgcgc cgccgccacg ctgcccgacg ggccggcaca gatcatcgcg cccgcgggca
     9301 gcggcaagac gacgaccatg gtcgcgcggc tggccgtgct cctggcccgc ggcgtcgcgc
     9361 ccgagcggat ctgcgtcgtc accttcaacc gggacgcggc gcaggacctg aaggcccggg
     9421 tcgaacgacg cctggcgtcc gtggtgcccc gtgcgcgaga gatcgagatc cgcaccctgc
     9481 acgcgctcgc ccgccaggtc gccctggatg ctggcgagcg gcgcgagatc gtcgccgacc
     9541 gcctgccgct cctgcgcgcg gcgcgccggc gcgtggcacg tgaggacgaa ccaccgccgc
     9601 cggaggccgc ggacctcgac acctggctct cggcgtggcg gatcgagggc cgtccgccgc
     9661 cggagcaggc ccgggccacc atcgcggcgt acgaggacct gctgcgagcc cgcggcctcc
     9721 tggatttcga cgatctcgtc gtgggcgccg ccgaccgcct cgagcgcgat ccccgcctgc
     9781 ggctgcgctg gcaggcgcgc ttcgcgcacg tgtgtgtcga cgagttccag gacgtggatg
     9841 cggcccagct gcggctggtc cggctgctcg ccgcccctga ggacaacctc ttcgtggtgg
     9901 gcgacgacga ccagacgatc tacgcgtggc gcctggctga cgtccggcga atcctgcgct
     9961 tctcggccga ttaccccgac gcccgtcgcg tcatgctggc gacgaactac cgatgcccga
    10021 ccgcggtcgt cgcggcatcc gcgcgtctcg tcgccgtcaa ccgggagcgc ttcgccaagc
    10081 cgatccgggc ggctccggac ctggccgacg atccccgcgc cattgaggca tggccgtcgg
    10141 gagatgccga gtggcccgac cacgccgcgc ggctggctgc ggatgccacc cgacggggac
    10201 ggcagatctg cttcctggcg cggacgcggg cagagctggt gccggtcttc ctggccctgg
    10261 tacgagccgg cgtgccccac cggcccaacg tcccgcccat cgtggaggcg gcgccggtga
    10321 cgacgctcgt ggacgcggca cgggccactc ccccgtccgc gccgccgttc cgggcactcc
    10381 tcgggctgcg cgcgggccac ggatggcggc gtggggaccc gggagccgac catctctccg
    10441 acgaggacca ggccgcgctc gacgccctgc tcggctggag tgtcgggttt cgacggctcg
    10501 gcgccttcgt gggtgccttc gacgccgcac gggcgcgcat ccacgccctg cagtccgacg
    10561 acgcggtcgt ggagctcgcg accgtccatg cgtcgaaggg acgggagtgg gagaccgtcg
    10621 tcctcgtcgg gttcgaggag ggccggatcc cgaaccggcg gtccatcgag gacgcagatg
    10681 attccgagcg cgcggtcgag gaggagcgcc gcctcgccta cgtggccctc acccgcgcga
    10741 cgcgccacct ggtgctggcc ttcgaccccg cgcgtccgtc gccgttcctc gcggagatgg
    10801 gattcgccct tcgtccggcg tccgcatcga gacgtttgtc gtaggaaccg gaccgcgcgc
    10861 gcctggcgtc tccgtggcgg gcagtgcgcc catccaacag cgacaccaca ggaaggaacc
    10921 ctcgatgtct ctcgctagtt cagtgcggca gcgtgcagcg cgcgccgcca tcgtctccct
    10981 cttcgtggtc ggtggcctcc ttgggctggc ggccgcggcg tctgcccaat cgccggacgt
    11041 cctccccgtc gagcccgatg gcggcatcgg cggcgaggtg gtcctgcccg tggagcccga
    11101 tggcggcatc ggcggcgagg tcgtcctccc cgtcgaaccc gatggcggca tcggcaactg
    11161 acgcttcatc cgcgtgaaac gcccccgaga cgatcctggg cgttccgcga tgcccgacgc
    11221 gggcaggcag gcgcccgccg ggccgccgcg gcccgctcag gacaccgatg ctgaaacccg
    11281 gcggtgcgac ttcggccgcg agggcgaagc agcgtcgcca tcggcgatcc cgagcaccgg
    11341 tcgcaggtac ttgccggtgt acgagccggc ggcacgggcc acctgctcgg gcgtgcctga
    11401 cgccaccagg cggccgccct tgtcgccgcc ctccggcccc aggtcgatca cccagtcggc
    11461 cgtcttgatg acgtcgaggt tgtgctcgat caccacgacc gtgttgccgg cctcgaccag
    11521 ccggccgagc accgacagca ggcgtgccac gtcgtcgaag tggaggccgg tggtcggctc
    11581 gtcgaggatg tagaaggtct tccccgtggc gcgcttgctc agctccgtcg cgagcttgac
    11641 ccgctgcgct tcgccaccgg agagcgtggt cgccggctgg cccaggtgga tgtaccccat
    11701 ccccacgtcc accagcgtct ggagcttgtt gcggatgctc ggcacggcgg agaagaagtc
    11761 cagcgcctcc tcgacggtca tctcgaggat ctccgccacg ttcttgccct tgtagtggat
    11821 ctcgagcgtc tcgcggttgt agcgcttccc cttgcagacc tcgcaggcca cgtagacgtc
    11881 gggcaggaag tgcatctcga tctggacgat gccggcgccc tcgcacgcct cgcagcgacc
    11941 gcccttgacg ttgaagctga agcgccccgg tttgtagcca cgcagccgcg cctccgggac
    12001 cgcggccagc agctcgcgga gcggggtcca caggccggtg tacgtcgctg ggttggagcg
    12061 cggcgtccgg ccgatgggcg actggtcgat ctcgatgacc ttgtcgatgt gctcgatccc
    12121 ctcgatccgg tcgtgcctgc ccgcgggctc tcgcgcgccc cagatgcgct gggccagcgc
    12181 cgggtacagg atctgggtca ccaggctcga cttgccggag ccgctcacgc cggtcacggc
    12241 cacgaaccgg cccagcggga aggccacgtc gatggcctgc aggttgttct cgcgggcgcc
    12301 gcggaccacc agcgcgtccc gctttccgtt gcggcgtcgc ttcggcaccg ggatgtgccg
    12361 ctcgccgcgc aggtacgcgc cggtgatgga tcgcggctcc gcaagcagcc ctgcgagatc
    12421 cccggagtag atgagctcgc cgccgtgctc ccccgcccgc ggaccgatgt cgatgaccca
    12481 gtcagcggcg cggatggtgt cctcgtcgtg ctcgaccacg agcaacgtgt tcccgaggtc
    12541 gcgcagccgc acgagcgtcg cgatcagccg gtcgttgtcg cgctggtgca ggccgatcga
    12601 cggctcgtcg aggatgtaga gcacgcccat cagcgaggac ccgatctgcg tcgccaagcg
    12661 gatgcgctgc gcctcgccgc ccgacagcgt ccccgacgcg cggtccagcg tcaggtagtc
    12721 cagccccacg tcgaccagga agcccaggcg cgcgcggatc tccttgagga cctggcgcgc
    12781 aatctggttc tcgcgatcgt tcaggcgttt ggggagcgcg tcgtaccagg ccagcgcatc
    12841 ggtcaccgag cgctgggtcg tctcgatgat gttgcggccg gccacggtga cggccagcga
    12901 ctccggcttg agccgcatcc ccttgcaggt cggacacggc ttggtggtca tgtagcgctc
    12961 gatctcggcg cgcatcgc
//
LOCUS       DASTYP010000165        10996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_340708, whole genome shotgun sequence.
ACCESSION   DASTYP010000165 DASTYP010000000
VERSION     DASTYP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340708"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1447
                     /locus_tag="VFV59_07580"
     CDS             44..1447
                     /locus_tag="VFV59_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU5325728.1"
                     /translation="MTRVEAEATEVPRSSAAAPPARGGLDAFFKLSERGTSVGTEVRA
                     GITTFLVMAYIIFLNPAILSNMFAADSAEQLAFIPAASAATALLAGVLTIAMGLVSNY
                     PIALAAGLGINAIVAFTLVLGLQLSPAGAMGVIVLEGLIILVLVIVGLREAIMDAVPL
                     VLKRAIGVGIGLFILFIGFVDGGLIVPGTPVVQFNFPVEPATWTFVLGLVLTIVLFAL
                     KVRAALLISIIVMSVFAYAVGLTSFPTDMSLVPDFSTLGKFDVTEVWNLGILAAALTI
                     FAIMLTDFFDTMGTATAITEQAGLTTPDGKVPGINRVLLVDSVAAAAGGAAGISSNTS
                     YIESAAGVAEGGRTGLTSVVTGLFFLAAVFVTPLATMVPFAATGPVLVVVGYLMATLI
                     KDIDFADIEEGFPALLGITLMPLTYNITVGIGAAFVTYVLIKAIRGKFGEIHWLLWVV
                     ALAFAVYFAQSWINSII"
     gene            1515..2576
                     /gene="queA"
                     /locus_tag="VFV59_07585"
     CDS             1515..2576
                     /gene="queA"
                     /locus_tag="VFV59_07585"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU5325729.1"
                     /translation="MTAPRLRVDDFDYELPAGAIAQTPAEPRDASRLLVLHRATGTLE
                     HAVFRDIGTWLRRGDLLVVNDSRVIPARLPATRASGGGAEILVLRQLDDPRHWEALVR
                     PSRRMGVGEAVTLRNGASVQVGERLGDGTRVVIFPDGPDPFELMAEAGEMPLPPYIHD
                     RSSPPDRYQTVYARPPGSAAAPTAGLHFTPELLESVERSGVERATVTLHVGLDTFRPL
                     EGAYVDEHRIHTEWYEVPDATRERIQRARDRGGRVVAVGTTAVRVLETAARTEAASGW
                     TDLYITPPYRFRSVDGLITNFHLPRSSLLLLVTAFVQAGMADGTDAYAARDVLLDAYR
                     TALVAGYRFFSFGDAMLIL"
     gene            complement(2597..3238)
                     /locus_tag="VFV59_07590"
     CDS             complement(2597..3238)
                     /locus_tag="VFV59_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325730.1"
                     /translation="MRLTEWPRMPFLNDEVKEWIALELQSLGVEDLAVYALEAGAEGD
                     ERRIMVATEVGLLDHRYAPFGSSARYRLAMLMYPWQVLRGVELRADTFRLWAREHRTR
                     WSFRLHHPHFEAATESPDLGQALCDLARVCAVMAEPFGVPAGRASSEVPGAHPGGPRP
                     LPPSGDAPPAPARVSGRDDGTAETTGETAPEEPPRDLSDEEREAIPTRSDDQE"
     gene            3293..4429
                     /gene="tgt"
                     /locus_tag="VFV59_07595"
     CDS             3293..4429
                     /gene="tgt"
                     /locus_tag="VFV59_07595"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009829106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5325731.1"
                     /translation="MRAPITSTFEATPVGRARLGRIVTPHGPIDTPQFMPVGTQATVK
                     ALTPGDLRAAGAQMILGNTYHLNLRPTAERIARLGGLHRFMSWDGPILTDSGGFQVFS
                     LADLRRVDDDGVTFISHLDGSTHRLTPERSMEIQALLGSDVAMAFDQLVDPGLPSHEV
                     EEAMERTHRWADRSLAARGRPDQAVFGIVQGGVDPSLRRRSAARIAGLPFDGIAVGGL
                     SVGESKAEMAATLDVVATALGDDPRPRYLMGVGSPVDFFTAVEHGIDLFDCVLPTRVA
                     RTGQVWTDAGRLNLRNSALQDDPGPLDPTCACEACRNHSRAYLAHLFRARELLAYRLA
                     SVHNVTWTLELLRRLRASLRDGSFDTLAASVRAAFSRGSASTRA"
     gene            complement(4445..5107)
                     /locus_tag="VFV59_07600"
     CDS             complement(4445..5107)
                     /locus_tag="VFV59_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEU5325732.1"
                     /translation="MKLLLTSGGVTNPSIRAALERLLPKPIGDCRALCIPTAQWGHPM
                     CGPWSVRGFVAAEPRWTHFSGLGWGSLGVLELTALPSIGVERWVPWVREADVLLADGG
                     DATYLSHWMRESGLADLLPSLPNTVWVGVSAGSMVMTPRIGSYFVEWPSAPDDRTLGI
                     VDFSIFPHLNAFPTNTPAHAERWAADIGGPTYAIDEQTAIVVADGAVEVVSEGTWTRF
                     DP"
     gene            5376..5555
                     /locus_tag="VFV59_07605"
     CDS             5376..5555
                     /locus_tag="VFV59_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325733.1"
                     /translation="MAKGRDRPRKEPKRKAKEKAPKQHPPGTAGWEPPRHDTPTRPVE
                     VIRPKRKEREPQDDE"
     gene            5601..6677
                     /gene="recA"
                     /locus_tag="VFV59_07610"
     CDS             5601..6677
                     /gene="recA"
                     /locus_tag="VFV59_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU5325734.1"
                     /translation="MDAPVVTRTQADGAAAKNGSGERSRAVDAAILQIEKQFGRGSIM
                     RLGDREAQNVPAISTGSVALDLALGVGGVPRGRITEIYGPESSGKTTLCYHIAATAQR
                     NGGIVAFIDAEHALDPGYAKNVGVNVDELLVSQPDTGEQALEIAETLIRSGGVDVVIV
                     DSVAALVPRAEIEGDMGDSFVGLQARLMSQALRKLTGAINRSQTALVFTNQLREKIGV
                     MFGNPETTPGGRALKFYASVRMDIRRIETLKQGTDAVGSRTRVKVVKNKVASPFRVAE
                     FDIMYNEGISREGGLLDVGIAAGILSKTGAWFNYGDVRLGQGRENARDFLKQNPEIAQ
                     KIDDEIRGRVASIEVGVEGISEAE"
     gene            6691..7245
                     /locus_tag="VFV59_07615"
     CDS             6691..7245
                     /locus_tag="VFV59_07615"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEU5325735.1"
                     /translation="MPQRGSRPSEPRDPPDAETALEIAVRFLGTRPRTRWEVERRLRR
                     AGAQPGVIEGVLERLSGLGYVDDVAFARWWGEQRDRHAPRGRRLVEAELRAHGVPRDV
                     IAAYVLEHADPERLPGDEVLPATEEERARDALDRHLRGRPVPTEPKALQRLGMFLMRR
                     GFDPGTARAAIRAAGAGVTDTEEA"
     gene            7251..7718
                     /locus_tag="VFV59_07620"
     CDS             7251..7718
                     /locus_tag="VFV59_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5325736.1"
                     /translation="MATSSAPSPEAYLAELGPERREIVGRIRDVVNANLPPGYREGMT
                     SGMIGWYVPLETFGDTYNGQPLGLAGIASQKQYVSLYLLNVYGDPATEAWFRDRWAAT
                     GKPLKMGKSCVRFRRLDDVPLDVIAETIARADLDGYLERYRAVAGSARRARRG"
     gene            7728..8681
                     /locus_tag="VFV59_07625"
     CDS             7728..8681
                     /locus_tag="VFV59_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5325737.1"
                     /translation="MRYALMTEPQQGLSYDEILAIVRTAEEAGLEAFFRSDHYASFPG
                     DSGLPTTDAWTTIGGLARETAAIHLGVLVSPVTFRIPGSLAKVIQTADEMSGGRIEAG
                     FGAGWNDEEHAQLGIPFPPIAERYDMLEEQMALIHGLWTEPDGWSYEGEHWQVRGAKR
                     HGEIARGGRRHPHIIFGGKGGPRLAALVARYGDEFNLNSAKPEDAGPAYARVRDACRE
                     VGRDPDELVYSAMTGVLVAETEEDLRTRVHDLMSMLGRAGADADDWLAERRGRWVMGT
                     PEQAHERVAALEAAGTQRVMLQDFLPRDLDHVRLMGRLFAT"
     gene            8805..10334
                     /gene="rny"
                     /locus_tag="VFV59_07630"
     CDS             8805..10334
                     /gene="rny"
                     /locus_tag="VFV59_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5325738.1"
                     /translation="MPELVVIVVAAVAAAIGIVFGFFVRRMVAANAVRHAEQYAERLV
                     AEARAKQKEIVLEGKDEALHLRRAAEEEAREQRSTLQRTERRLLDREEALDRKLTALE
                     EREAALAGRQAELEDERRRLSEQQERQRAELERISGLTAAEARQTLIAQIVDEAQAEA
                     QHRVREIEHRAVEEGEDRARRVLTTVMQRIAADHTSEATVTVVNLPNEEMKGRIIGRE
                     GRNIRALEQATGVDLIIDDTPEAVVLSGFDPVRREVARMALNKLISDGRIHPGRIEET
                     VAKCRQEIEVVMRQAGEQAAYDAGVPGLHPELIKLLGRLKYRTSYGQNVLNHCVETAR
                     LAGLLAAEIGANVSDAKKGGLLHDIGKAVDHEVEGPHAAIGGEIAARYGITPVVCNAI
                     AAHHQEVEQESVEATVVQIADAISASRPGARGETLDNYVKRLDDLQQIALGFPGVERC
                     YAIQAGREIRILVRPEEMDDLAASRLARDVVRKIEEQLQYPGQIKVTVIRETRAVDYA
                     R"
     gene            10331..>10996
                     /locus_tag="VFV59_07635"
     CDS             10331..>10996
                     /locus_tag="VFV59_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEU5325739.1"
                     /translation="MSDLRIGVIGDIIGKPGRQAVAHILPELRDEFALDMVIANGENT
                     AAGAGLTPSLAEELLAGGVDVITSGNHIWDKREIYEYLDSDRPVLRPINYPDDAPGRG
                     WLVHHTDAGDEVAVINAMGRVYMNQLDSPFTKLDDLLDGAAMPLPPLRILDFHCEITS
                     EKNAMGWYLDGRVSAVVGTHTHVPTADARVLPNGTAYISDIGMTGPRDSVIGFALDTV
                     LPRF"
BASE COUNT         1689 a         3861 c         3896 g         1550 t
ORIGIN      
        1 gcgcggtgtc gtgcgctcca ggtgcccagt ggagacgcgg agggtgaccc gagtggaggc
       61 agaggcaacg gaggtaccga ggtcgagcgc ggcagcgcca ccggcccggg gagggctcga
      121 cgccttcttc aagctcagcg agcgggggac gtccgtcggc acggaggtcc gggcgggcat
      181 caccaccttc ctggtgatgg cctacatcat cttcctgaac cccgcgatcc tctcgaacat
      241 gttcgccgcc gactcggccg agcagctggc gttcatcccg gcggccagcg cggcgacggc
      301 gctgctcgcg ggcgtgctca cgatcgccat ggggctcgtc tcgaactacc cgatcgccct
      361 cgccgcgggc ctggggatca acgccatcgt cgcgttcacg ctcgtgctgg gactgcagct
      421 ctcgccggcc ggtgcgatgg gcgtgatcgt gctcgagggc ctcatcatcc tggtgctcgt
      481 catcgtcggc ctgcgcgagg cgatcatgga tgcggtgccg ctcgtgctga agcgcgcgat
      541 cggcgtcggc atcggcctct tcatcctgtt catcggcttc gtggatggcg gcctgatcgt
      601 ccccggaacg ccggtcgtgc agttcaactt cccggtcgag cccgcgacgt ggacgttcgt
      661 gctcggcctg gtcctgacca tcgtgctgtt cgccctgaag gtgcgcgcgg ccctgctcat
      721 cagcatcatc gtcatgtcgg tgttcgccta cgcggtcggg ctgacctcgt tcccgaccga
      781 catgagcctg gtgcccgact tctcgaccct cgggaagttc gacgtgaccg aggtctggaa
      841 cctgggcatc ctggcggcgg ccctgaccat cttcgcgatc atgctcacgg acttcttcga
      901 cacgatgggc acggccacgg ccatcacgga gcaggcgggc ctgaccacgc ctgacggcaa
      961 ggtgccgggc atcaaccgcg tgctgctggt ggactcggtc gccgcggcgg ccggtggtgc
     1021 cgccggcatc agctcgaaca cgagctacat cgagagcgcg gccggtgtcg cggaaggcgg
     1081 tcggaccggc ctcacctcgg tcgtgacggg tctcttcttc ctcgccgccg tgttcgtcac
     1141 gccgctggcc accatggtgc cgttcgccgc caccgggccg gtcctggtcg tggtcggcta
     1201 cctgatggcg accctcatca aggacatcga cttcgcggac atcgaggagg gcttcccggc
     1261 cctgctcggc atcacgctga tgcccctgac gtacaacatc acggtcggca tcggtgccgc
     1321 cttcgtgacg tacgtgctga tcaaggcgat ccgcggcaag ttcggtgaga tccactggct
     1381 gctatgggtc gtggcgctgg ccttcgcggt gtacttcgcc cagagctgga tcaactcgat
     1441 catctagcgc tccgtcgaaa cggaccgatg gcgcccggtc ggcctcgccg cccgggcgct
     1501 acgctgtccg gcgcatgacc gcgccccgcc tgcgcgtcga cgacttcgac tacgagctgc
     1561 ccgccggcgc catcgcccag accccggccg aaccccgtga cgcctcgcgc ctgctcgtgc
     1621 tccaccgcgc gaccgggaca ctcgagcacg cggtcttccg ggacatcggg acctggctgc
     1681 ggcgcggcga cctgctcgtc gtcaacgact cacgcgtgat ccccgcgcgg ctgcccgcga
     1741 cccgcgcgtc cggtggcggc gccgagatcc tggtgctgcg gcagctcgac gacccgcggc
     1801 actgggaggc gctggtgcgc ccgtcccggc gcatgggcgt cggggaagcg gtcacgctgc
     1861 gcaacggcgc cagcgtccag gtgggggagc ggctgggaga cgggacccgc gtcgtgatct
     1921 ttccggatgg cccggacccg ttcgagctga tggcggaggc cggcgagatg ccgctgccgc
     1981 cgtacatcca tgaccgatcg tcgccgccgg accgctacca gacggtctac gcccggccac
     2041 cgggcagcgc cgcggcgccg accgcagggc ttcacttcac gccggagctg ctggagagcg
     2101 tcgagcgctc cggcgtggag cgagcgacgg tcacgctcca cgttgggctc gacacgttcc
     2161 gtccgctcga gggcgcctac gtcgacgagc accgcatcca caccgagtgg tacgaggtgc
     2221 cggacgccac ccgggagcgg atccagcgcg cgcgggaccg cggcggccgc gtggtggcgg
     2281 tcggcaccac ggccgtgcgc gtgctggaga ctgcggcgcg cacggaggcc gcgtcgggct
     2341 ggacggacct gtacatcacg ccgccgtatc ggttccgcag cgtcgacggc ctgatcacga
     2401 acttccacct gccgcgtagc tcgctgctgc tgctggtgac ggccttcgtc caggcgggta
     2461 tggccgacgg aaccgatgcc tacgcagcgc gtgacgtcct gctggatgcc taccggacgg
     2521 cactggtcgc ggggtaccgg ttcttcagct tcggcgacgc gatgctgatc ctctgaccgg
     2581 ggcgagctcg gcgcgactac tcctggtcgt cggagcgggt cgggatggcc tcgcgctcct
     2641 cgtcgctgag gtcgcgcggt ggctcctcgg gtgcggtctc ccccgtggtc tccgcggtgc
     2701 cgtcgtcgcg cccgctcacc ctggccgggg cgggaggcgc gtcgccggac gggggcagcg
     2761 ggcgcgggcc gcccggatgg gcacccggga cctcggagga cgcacggccc gcgggaacgc
     2821 cgaacggctc ggccatgacc gcgcagacgc gggccaggtc gcagagggcc tgccccaggt
     2881 ccggtgactc ggtggccgcc tcgaagtgcg gatgatggag ccggaacgac cagcgcgtgc
     2941 ggtgctcgcg cgcccacagc cggaacgtgt cggcgcggag ctcgacgccg cgcagcacct
     3001 gccaggggta catcagcatc gcgaggcggt agcgcgccga cgagccgaag ggcgcgtacc
     3061 gatggtcgag cagaccgacc tcggtggcca ccatgatccg gcgctcgtca ccctcggcac
     3121 cggcctccag cgcgtagacc gccaggtcct cgacgcccag gctctgcagt tcgagcgcga
     3181 tccattcctt cacttcgtcg ttcaggaacg gcatgcgcgg ccattcggtc agtcgcacgc
     3241 gacggtcacc tctccgtagt gcatccgtag cctatcctct ccggcggtgt cagtgcgggc
     3301 tcccatcacc tccaccttcg aggccactcc ggtcgggcgc gcccggttgg gtcgcattgt
     3361 cacacctcac gggccgatcg acacgcccca attcatgccc gtcggcacgc aggccaccgt
     3421 caaggcgctg acgcccggtg acctgcgcgc cgccggggcg cagatgatcc tgggcaacac
     3481 ctaccacctc aacctgcggc cgaccgcgga acgcatcgcc cgcctgggcg ggctgcatcg
     3541 gttcatgagc tgggacggac cgatcctgac cgattccggc ggcttccagg tcttcagcct
     3601 tgctgacctc cggcgggtcg acgacgacgg cgtgaccttc atttcgcacc tcgacggctc
     3661 gacccatcgg ctcacgcccg agcgctcgat ggagatccag gcgctcctcg gctcggacgt
     3721 cgcgatggcc ttcgaccagc tggtcgaccc ggggcttccg tcgcacgagg tcgaggaggc
     3781 gatggagcgg acgcaccgct gggccgaccg ctcgctggcg gcccgcgggc gcccggacca
     3841 ggccgtgttc ggcatcgtgc agggcggtgt cgatccgtcg ctccgtcggc gctccgcggc
     3901 gcgcatcgcc ggactgccgt tcgacggcat cgcggtcggc gggctctcgg tgggggagtc
     3961 caaggccgag atggcggcca cgctggacgt ggtggccacc gcgctcggcg acgatccgcg
     4021 gccgcgctac ctgatgggtg tcggctcgcc ggtcgatttc ttcaccgcgg tcgagcacgg
     4081 catcgatttg ttcgactgcg tcctgccgac gcgggtcgcc cgcacgggcc aggtctggac
     4141 cgatgccggc cggctcaacc tccgcaactc ggcgctccag gacgacccgg gcccgttgga
     4201 cccgacctgc gcctgcgagg cgtgccggaa ccattcgcgg gcgtacctgg cccacctgtt
     4261 ccgggctcgc gagctgctcg cgtaccggct ggcgtcggtg cacaacgtga cctggacgct
     4321 cgagctgctg cgccggctgc gcgcatcgtt gcgcgacggc tcgttcgaca cgctggccgc
     4381 atcggtgcgt gcggccttca gccgaggctc ggcgtcgacc agggcgtgat ccccacggcc
     4441 ccagctacgg gtcgaacctc gtccacgtcc cctcggacac gacctcgacg gcgccgtcgg
     4501 cgaccacgat cgcggtctgc tcgtcgatgg cgtacgtcgg gccgccgatg tcggccgccc
     4561 agcgctccgc gtgtgccggc gtgttggtgg gaaacgcatt gaggtgcgga aagatcgaga
     4621 agtccacgat ccccagggtt cggtcatcag gtgcggacgg ccattcgacg aagtacgagc
     4681 cgatccgggg cgtcatcacc atgctgccgg cgctcacgcc cacccacacg gtgttgggca
     4741 gcgatggcag cagatccgca aggcccgact cgcgcatcca gtggctcagg tacgtcgcgt
     4801 cgccgccgtc cgccaggagg acgtccgcct cgcgaaccca ggggacccac cgttcgacgc
     4861 cgatgctcgg gagcgccgtg agctcaagga cgccgagcga tccccagccg agccccgaga
     4921 agtgggtcca ccgcggctcc gcggcgacga agccgcggac tgaccagggt ccgcacatcg
     4981 ggtgccccca ctgggccgtc gggatgcaga gtgcgcggca gtcgccgatc ggcttcggga
     5041 gcaggcgctc gagggccgcg cggatgctcg gattcgtgac gccgccggac gtgagcagga
     5101 gcttcaaggt gcctccagga tcgtggtgat ggatggccgg tgtccgtagg accggtgagg
     5161 gcgcgggaac tcagcgcgga gcgccgagcc gtgcgtcata cgccgtatga catcggtgta
     5221 gaatgggccg tccgatcgtg acagggtagc tgtcgcgccc gagcgtagaa tcgggcccac
     5281 atgcggcgag tggtaagccg ggagtaagcg agaggtaacg gtcggcggcg catgatcggc
     5341 gccagctcga ccggctcacc acggaggttc gagcgatggc aaagggccgg gatcgaccac
     5401 gcaaggagcc gaagcgcaag gcgaaggaga aggcgccgaa gcagcatcca ccggggaccg
     5461 ccggctggga gccgccgcgg catgacacac cgacgcgccc cgtcgaggtg attcggccca
     5521 agcgcaagga acgggagccc caggacgacg agtgagcgga agccaccggg gccgaagagg
     5581 aacgaggagc aacacgtgac atggacgcac cggtggtgac ccgaacccag gcggacggcg
     5641 cggcggcgaa gaacggcagc ggcgagcgga gccgcgcggt ggatgccgcc atcctgcaga
     5701 tcgagaagca gttcggccgc ggctcgatca tgcggctcgg tgaccgcgag gcccagaacg
     5761 tcccggccat ctcgaccggg tcggtggctc tcgacctcgc gctcggcgtc ggcggcgtgc
     5821 cccgcggccg catcacggag atctacggtc ccgagtcgag cggcaagacg acgctgtgct
     5881 accacattgc ggcgacggca cagcgcaatg gcggcatcgt cgccttcatc gacgcggagc
     5941 acgccctgga tcccggctat gccaagaacg tcggcgtcaa cgtcgacgag ctgctcgtca
     6001 gccagcccga caccggggag caggcgctcg agatcgccga gaccctcatc cgctccggcg
     6061 gcgtcgacgt cgtcatcgtc gactcggttg cggccctcgt gccgcgcgcc gagatcgagg
     6121 gcgacatggg cgacagcttc gtcggcctcc aggcgcggct catgagccag gcgctgcgca
     6181 agctgaccgg cgccatcaac cgcagccaga cggcgctcgt cttcaccaac cagctgcgcg
     6241 agaagatcgg cgtcatgttc ggcaatcccg agactacgcc gggtggccgc gccctgaagt
     6301 tctacgccag tgtgcgcatg gacatccgac gcatcgagac cctcaagcag gggaccgatg
     6361 cggtcggctc gcgcacgcgc gtgaaggtgg tgaagaacaa ggtggcctcg cccttccgcg
     6421 tggccgagtt cgacatcatg tacaacgagg gcatcagccg cgagggcggc ctgctggacg
     6481 tcggcatcgc cgccggcatc ctgagcaaga ccggcgcgtg gttcaactac ggcgacgtgc
     6541 gcctcggcca gggtcgcgag aacgcgcgcg acttcctgaa gcagaacccc gagatcgccc
     6601 agaagatcga cgacgagatt cgcggacgcg tcgcgagcat cgaggtcggc gtcgagggga
     6661 tcagcgaggc ggagtaggga agcgcacccc atgcctcagc gcggctcgcg tccgtccgag
     6721 ccgcgcgacc cacccgacgc cgagacggcc ctcgagatcg ccgttcggtt cctgggcacg
     6781 cgcccgcgga cccgatggga ggtcgagcgc cgtctccggc gggccggtgc ccaacctggg
     6841 gtcatcgagg gcgttctcga gcggctgtcc ggcctgggct acgtcgacga tgtcgccttc
     6901 gcgcgctggt ggggggagca acgggatcgg cacgctccgc gcggccgccg gctggtggag
     6961 gccgagctgc gtgcccacgg cgtcccgcgc gacgtgatcg cggcgtacgt cctcgaacac
     7021 gccgatccgg agcggcttcc cggcgacgag gtcctccccg cgacggagga ggagcgcgcg
     7081 cgtgacgcgc tcgaccggca cctgcggggc cgtcccgtac ccaccgaacc caaggcactg
     7141 caacgcctgg ggatgttctt gatgcgccgc ggcttcgatc cggggaccgc gcgggcggcg
     7201 atccgggccg cgggcgctgg cgtgaccgac accgaggagg cgtgaccgac atggcgacca
     7261 gcagcgcgcc gagtcccgag gcgtacctcg ccgagctcgg cccggagcga cgcgagatcg
     7321 ttggccggat ccgggacgtc gtcaacgcga atctgccgcc cggctaccgc gagggcatga
     7381 cctcggggat gatcggctgg tacgtgccac tcgaaacctt cggcgacacc tacaacggtc
     7441 agccgctcgg cctggcgggc attgccagcc agaagcagta cgtgagcctc tacctgctca
     7501 acgtgtacgg ggatcccgcg acggaggcat ggttccgcga ccgctgggcc gcgacgggca
     7561 agccactgaa gatgggcaag agctgcgtgc gcttccgccg gctggacgac gtacccctcg
     7621 acgtcatcgc cgaaacgatc gcgcgtgccg acctcgacgg ctacctcgaa cgctatcgag
     7681 cggtggccgg ctcggcgcgg cgcgcccgcc gagggtaggc tggtctgatg cgctacgccc
     7741 tcatgaccga gccccagcag gggctcagct acgacgagat cctcgccatc gtgcggacgg
     7801 ccgaggaggc cggcctcgag gcgttcttcc ggtccgatca ctacgccagc ttccccggcg
     7861 actccggcct tccaaccacg gatgcctgga cgaccatcgg gggcctcgcc cgcgagacgg
     7921 ccgcgatcca cctgggtgtc ctggtatcgc cggtgacgtt ccgtatcccg ggcagcctgg
     7981 ccaaggtgat ccagacggcc gacgagatga gcggcgggcg catcgaagcc gggttcggcg
     8041 ccggatggaa cgacgaggag cacgcgcagc tgggcatccc gttcccgccc atcgccgagc
     8101 gctacgacat gcttgaggag cagatggcgc tgatccacgg gctgtggacc gagcccgacg
     8161 gctggagcta cgagggcgag cactggcagg tacggggcgc gaagcggcac ggcgagatcg
     8221 cacgcggtgg acggcgccac ccgcacatca tcttcggcgg caagggcggg ccgcgattgg
     8281 cggcactggt ggcgcggtac ggcgacgagt tcaacctgaa ctcggccaag cccgaggacg
     8341 ccggccctgc ctatgcgcgg gtccgcgacg catgccgcga agtcggccgc gaccccgacg
     8401 agctcgtcta ctcggccatg accggggtcc tggtggcgga gaccgaggag gaccttcgca
     8461 cgcgcgtcca cgacctgatg tccatgctcg gccgcgcggg cgctgatgcc gacgactggc
     8521 tggcggaacg ccggggacga tgggttatgg gcacgcccga gcaggcccac gagcgcgtcg
     8581 cggcactcga ggcggccggc acgcagcgcg tcatgctgca ggacttcctg ccccgagacc
     8641 tcgatcacgt tcggctcatg gggcggctct tcgccacctg acccaccggc tgagccctgc
     8701 gccgagttgc gctacactcg gccgacatca tcgactgacc gatgcgcccg ccgcgcgggc
     8761 gccatctcgc ccatccgggc tccaaccatc aagggggagc cgacatgcct gaactggtcg
     8821 tcatcgtggt ggcggcggtc gccgccgcca tcgggatcgt cttcggattc ttcgtgcgtc
     8881 gcatggtcgc ggccaacgcc gtgcgtcatg ccgagcagta cgcggaacgg ctcgtcgccg
     8941 aagcccgagc caagcagaag gagatcgtcc tcgaggggaa ggacgaggcg ctccacctgc
     9001 gccgtgcggc ggaggaggag gctcgcgagc agcgctcgac gctccagcgc accgagcgcc
     9061 ggctgctgga ccgggaggag gcgctcgacc gcaagctcac cgccctcgag gagcgcgagg
     9121 cggcgctcgc cggccggcag gccgagctcg aggacgaacg gcgccgcctc tccgagcagc
     9181 aggagcggca gcgggccgag ctggagcgca tctcgggcct caccgcagcc gaggcgcgtc
     9241 agaccctgat cgcccagatc gtcgacgagg cgcaggccga ggcgcagcac cgggtccgcg
     9301 agatcgagca ccgtgcggtg gaggaggggg aggaccgcgc caggcgggtg ctcaccaccg
     9361 tgatgcagcg catcgcggcg gaccacacgt cggaggcgac ggtgacggtc gtcaacctgc
     9421 cgaacgagga gatgaagggc cgcatcatcg gccgcgaggg ccgcaacatc cgcgccctcg
     9481 agcaggccac cggcgtcgac ctgatcatcg acgacacgcc cgaggccgtc gtcctctccg
     9541 gcttcgaccc ggtgcggcgc gaggtcgcgc gcatggcgct caacaagctg atcagcgacg
     9601 ggcgcatcca ccccggccgg atcgaggaga ccgtggccaa gtgccgccag gagatcgagg
     9661 tcgtgatgcg ccaggccggc gagcaggccg cgtacgacgc cggcgtgcct ggtctccacc
     9721 ccgagctcat caagctcctc ggccgcctca agtaccgcac cagctatggc cagaacgtgc
     9781 tgaaccactg cgtcgagacc gcgcgccttg ccgggctgct ggcggccgag atcggcgcca
     9841 acgtgagtga cgccaagaag ggcgggctgc tgcacgacat cggcaaggcg gtcgaccacg
     9901 aggtcgaggg cccgcacgcg gccatcggcg gcgagatcgc ggcgcgctac gggatcacgc
     9961 cggtggtgtg caacgcaatc gcggcgcacc accaggaggt cgaacaggag tcggtcgagg
    10021 cgaccgtggt ccagatcgcc gacgccatca gcgcctcccg accgggtgcg cgcggcgaga
    10081 cgctcgacaa ctacgtcaag cgcctggatg acctgcagca gattgccctc ggcttcccgg
    10141 gcgtcgagcg ctgctacgcc atccaggccg gtcgcgagat ccggatcctg gttcgcccgg
    10201 aggagatgga cgacctggcc gccagccggc tggcgcggga cgtggtcagg aagatcgagg
    10261 agcagctgca gtaccccggc cagatcaagg tgacggtcat ccgggagacc cgcgcggtcg
    10321 actacgctcg gtgagcgacc tgcgcatcgg ggtcatcggc gacatcatcg gcaagcccgg
    10381 ccgccaggcg gtcgcccaca tcctgcccga gctgcgcgac gagttcgccc tggacatggt
    10441 gatcgccaac ggcgagaaca ccgccgccgg cgccggcctg accccgagcc tcgccgagga
    10501 gctgctggcc gggggcgtgg acgtcatcac cagcggcaac cacatttggg acaagcgcga
    10561 gatctacgag tacctggact cggaccggcc cgtgctgcgc cccatcaact atccggacga
    10621 cgcgccgggt cggggctggc tggtgcacca caccgatgcc ggcgacgagg tcgcggtcat
    10681 caacgccatg ggccgggtct acatgaacca gctcgactcg ccgttcacca agctcgacga
    10741 cctcctcgac ggggcggcca tgccgctgcc acccctacgg atcctggact tccattgcga
    10801 gatcacgtcg gagaagaacg cgatgggctg gtacctcgac gggcgcgtga gcgccgtcgt
    10861 cggcacgcac acgcatgtcc cgacggccga tgcgcgcgtc ctgcccaacg gcaccgccta
    10921 catcagcgac atcggcatga ccgggccgcg cgactcggtc atcgggttcg cactggacac
    10981 cgtgctgccg cgcttc
//
LOCUS       DASTYP010000166         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_814239, whole genome shotgun sequence.
ACCESSION   DASTYP010000166 DASTYP010000000
VERSION     DASTYP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814239"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFV59_07640"
     CDS             complement(<1..397)
                     /locus_tag="VFV59_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325740.1"
                     /translation="MRGRSLAVRLAAVLAVIVVVVLLVAGLVVNRVTSRTIDETLGPR
                     EQQQLELAGDIIEGGLERGARGPGLRMLLQQLARQTRGEVRLVAPDGTVRFAVGDVPA
                     GVQTESLTRELSSAAGGGALEIEVPSARAG"
     gene            complement(397..1116)
                     /locus_tag="VFV59_07645"
     CDS             complement(397..1116)
                     /locus_tag="VFV59_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5325741.1"
                     /translation="MTPRARILVVEDEPQLRSLLRLYLEREGYEVTDAGDGRAALAAA
                     GNGDFDLVVLDLMLPGMQGEAVLAELRELGEVPVLISSAKRSDAERIAGLRAGADDYL
                     AKPFNPHELTARVAAILRRTRGSAEHRPPPILSLDGGRVVLDPASRRYTLRDGGDGRL
                     TPGECSLLVALADHPGAVLGRQQLLDAVARRPDEVYERVVDVHVANLRRKLGDDAGDP
                     WLIETVPATGYRLVASRDPEP"
     gene            1246..2037
                     /locus_tag="VFV59_07650"
     CDS             1246..2037
                     /locus_tag="VFV59_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325742.1"
                     /translation="MTLPKLLGGAGALILSALVGGTLISSALATDETADPSTDTTGDQ
                     VAYCEVFRDTLASELGVTTDELVSAGKAAANAAIDAAVAAGDLDEERATTMRERIDAF
                     DGDCAGLGRGFGLGFGMGFERGMERGLHLGLRFEAAADALGMTPPALFQALTDAGSLQ
                     AVADAQGVAYDTVTSAVLDDVQARLDEAVADGLDQDRADTILENLTTWLHGGGQLDEL
                     PSAGMGPGFAPGHGRGHGGMGPGMGPGFDPWGGDDDTDSGEDGGA"
     gene            2290..3279
                     /locus_tag="VFV59_07655"
     CDS             2290..3279
                     /locus_tag="VFV59_07655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5325743.1"
                     /translation="MTKIGYACMLEQFHPTELLDWAEQAEGAGFDAGFQVSEHFHPWT
                     PQQGQSAFAWSFMGALGQRTSLPFGTAVTCPGFRYHPAVIAHAAATLGAMYPGRFWLG
                     LGAGEALNEHVIGGEWPEIGIRSAMMFEAIEVINKLFSGKVVKHDGEYFTLESAKLYT
                     RPEQPVPIYVATAGPVNAKRTGKFADGIITVGAADEKINMLWDKFREGAQEAGKDASA
                     MRTQLQIHVSWAPTQEEAERNALEEWPNGGMPFPKQDIKNPEDFANIAKLVRIENFAN
                     RMLISADLEEHTAQIQKFVDMGFDEIYLHNVGRDQAAFIETFGTRVLPNLKLS"
BASE COUNT          518 a         1189 c         1124 g          454 t
ORIGIN      
        1 agccggcccg agcgctgggc acctcgatct cgagcgcgcc gcccccggcg gcggacgaga
       61 gttcacgcgt gagcgattcg gtctggacac ccgccggaac atcgccgacg gcgaaccgaa
      121 ccgtgccgtc cggcgcgacc aggcgaacct cgccacgcgt ctggcgagcg agctgttgga
      181 gcagcatccg cagtccgggt ccacgcgcgc cccgctccaa cccgccctcg atgatgtcgc
      241 cggccagctc gagctgctgc tgctcgcgcg gcccgagcgt ctcgtcgatg gtgcgtgacg
      301 tgacgcggtt caccaccaac cccgccacca gcagcacgac gaccacgatc accgcgagca
      361 cggcggccaa ccggaccgcc agcgaacgtc cccgcatcac ggttccggat ccctgctcgc
      421 cacgagccgg tagccggtcg ccgggacggt ctcgatcagc cacggatcac cggcgtcgtc
      481 gccgagcttg cggcgcaggt tggcgacatg gacgtcgacc acgcgctcgt acacctcgtc
      541 cgggcgccgc gccacggcgt ccaggagctg ctgccggccg aggacggcgc ccgggtggtc
      601 cgccagggca accaggaggc tgcactcgcc cggcgtcagc cgtccgtcac cgccgtcccg
      661 gagggtgtat cgacgcgacg ccgggtcgag gacgacgcgt ccgccgtcca ggctgaggat
      721 cggcggtggc cgatgctcgg cgctcccgcg cgtgcgccgc aggatcgcgg ccacgcgggc
      781 ggtgagctcg tgcgggttga acggcttggc caggtagtca tcggcaccgg cgcgcaaccc
      841 ggcgatgcgc tcggcgtccg aacgcttcgc ggacgagatg aggacaggga cctcgcccag
      901 ctcgcggagc tcggcgagga ccgcctcacc ctgcatcccc gggagcatca ggtcgagcac
      961 caccagatcg aagtcaccgt tcccggcggc ggccagcgcc gcacggccgt cgccggcatc
     1021 ggtcacttcg tagccctcac gctccaggta cagccggagc agcgagcgga gctgcggctc
     1081 gtcctccacg acgaggatgc gcgctcgggg ggtcatggcc gcatcctacg cgcggcccgt
     1141 tcagacgatg tgaagacggg ccatgcggat cttcgcgcga tcttcatggc ggcggatgat
     1201 cctgacgggc atcggacacc acgcacctga ggttcaacca ccaccatgac gctcccgaag
     1261 ctcctcggcg gcgccggggc actcatcctt tccgccctgg tgggcggcac cctgatctca
     1321 tcggcgctcg ccaccgatga gacggccgat ccgagcaccg atacgaccgg tgaccaggtc
     1381 gcctactgcg aggtcttccg cgacacgctc gcctccgagc tgggcgtgac gaccgacgag
     1441 ctcgtctccg ccgggaaggc ggcggccaac gcggcgatcg atgccgccgt cgcggctggc
     1501 gatctcgacg aagagcgcgc gacgaccatg cgcgagcgga tcgacgcctt cgacggcgac
     1561 tgcgccggcc tcggtcgcgg gttcgggctg ggcttcggca tgggcttcga gcgcggcatg
     1621 gagcgtgggc tccacctcgg cctccgattc gaggccgccg ctgacgcgct cggcatgacg
     1681 ccgccggccc tgttccaggc actgaccgat gccggctcgc tgcaggcggt cgccgatgcc
     1741 cagggcgtcg catacgacac cgtcacgtcc gccgtcctcg acgacgtcca ggctcgcctc
     1801 gacgaggccg tcgccgacgg gctcgaccag gatcgtgcgg acaccatcct cgagaacctc
     1861 acgacctggc tgcatggcgg cggtcagctc gacgagctgc cgagcgccgg gatgggtcca
     1921 ggcttcgccc cgggtcacgg tcgcggccac gggggcatgg gtccgggaat gggcccgggc
     1981 ttcgacccgt ggggtggcga cgacgacacg gattccggtg aggacggcgg agcgtagcct
     2041 ccgtcctcct ggttcgaggc gccggatgcc agtggctccg gcgccgcgct gttgccaccc
     2101 ggtgctgctc ctgacaggag cattgcgtcc gtcgaaggac ccgatctgcg tcctcgcact
     2161 cgtgaggcgc tcgtcacgcg agcgccgtga ccgccgacgc gatactcgcc ggacgaagtg
     2221 ctcctctggc gatcgacccc cgggcggggc accccacgcg ggcgcccggt cgccgctacg
     2281 cttaccggca tgacgaagat cggatacgcg tgcatgctcg agcagttcca tcccacggag
     2341 ctgctcgact gggccgaaca ggctgagggg gcaggattcg acgctggctt ccaggtcagc
     2401 gagcacttcc acccgtggac cccgcagcag ggccagtccg cgttcgcgtg gagcttcatg
     2461 ggcgcgctgg gccagcgcac cagcctcccg ttcggcaccg cggtgacgtg ccccggcttc
     2521 cgctaccacc ccgccgtcat cgcgcacgcg gccgccacgc tgggcgccat gtaccctggc
     2581 cgcttctggc tgggcctggg cgccggtgag gcgctgaacg agcacgtcat cggcggtgag
     2641 tggccggaga tcggcatccg gtccgcgatg atgttcgagg ccatcgaggt catcaacaag
     2701 ctgttcagtg gcaaggtcgt caagcacgac ggtgagtact tcaccctcga gtccgcgaag
     2761 ctctacaccc gcccggagca gcccgtcccg atctacgtgg cgaccgccgg ccccgtgaac
     2821 gccaagcgca ccggcaagtt cgccgacggg atcatcaccg tcggggcggc cgacgagaag
     2881 atcaacatgc tctgggacaa gttccgcgag ggcgcccagg aggccggcaa ggacgcatcg
     2941 gccatgcgca cgcagctcca gatccacgtg tcctgggcgc ccacccagga ggaggcggag
     3001 cgcaacgcgc tggaggagtg gcccaacggc ggcatgccgt tcccgaagca ggacatcaag
     3061 aaccccgagg acttcgccaa catcgccaag ctcgtgcgga tcgagaactt cgccaaccgg
     3121 atgctgatca gcgctgatct cgaggagcac accgcccaga tccagaagtt cgtggacatg
     3181 ggcttcgacg agatctacct gcacaacgtc gggcgcgacc aggcggcgtt catcgagacc
     3241 ttcggcacca gggtgctgcc gaacctgaag ctgagctgac ggccg
//
LOCUS       DASTYP010000167         7395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_632930, whole genome shotgun sequence.
ACCESSION   DASTYP010000167 DASTYP010000000
VERSION     DASTYP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632930"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFV59_07660"
     CDS             complement(<1..1043)
                     /locus_tag="VFV59_07660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5325744.1"
                     /translation="MATRTVDRAAANQEPTPITTVRPVKANPLREGLRLERMAEPCTM
                     VICGATGDLTERKLGPALYNTLLGGFLPPEFTVVGFARRDLTDEQFREHLLEGINKYS
                     RNRPAKPAIWDSFAKGIEYHRGDFDDPAAFVELAKRLDRIDRDRGTQGNRLFYLAVPP
                     NLYPEIVNQLDRAGLAASGAHRRTGAKRGWTRVIVEKPFGYDLDSARRLNHEIAEVFD
                     EGQVYRIDHYLGKETVQNLAVFRFGNGLFEPIWNRRYIDSVQITVAETVGVEGRGEFY
                     DQTGALRDIVQNHGLQLMAMFAMEPPVEFAAEDLRDEKLKVLRGVKPMTPEEVAANTV
                     RGQYVSGWVEGEKV"
     gene            complement(1050..1970)
                     /gene="gnd"
                     /locus_tag="VFV59_07665"
     CDS             complement(1050..1970)
                     /gene="gnd"
                     /locus_tag="VFV59_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEU5325745.1"
                     /translation="MQVAICGLGRMGAGMARRAARGGHDVVVWNRTESVASEVAAEPE
                     NEGRVSVAEPLDRLVEMLPAPRHVIVSVPSGAATDAMIEQLAELLEPGDVIIDAGNSR
                     FHDSKRHAAELAEKGIEWLDMGVSGGVWGLQVGFCAMLGGKREVFDRFEPVVKTLAPE
                     GGYLYCGDAGAGHYVKMVHNGIEYGIMQAYGEGFDILHASEYDLDLAAVARMWNNGSV
                     IRSWLLELAGNAFEREGNDLDEIRGWVADSGEGRWTVAEAIDHDVPAPVITLSLLQRF
                     RSRRDPDSYSDRVLAALRNEFGGHAIKERE"
     gene            complement(1970..3706)
                     /locus_tag="VFV59_07670"
     CDS             complement(1970..3706)
                     /locus_tag="VFV59_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU5325746.1"
                     /translation="MTGVEDAVEARLERLTGERVAERLWARDGSLWAESQKKPDEVAA
                     WLGWLALPDAMSERVTELEHLARAVREDGYRRAAVLGMGGSSLAPELFSRVFGWAGGP
                     AGTGAASADGLELRILDSTHPDVVRGFRSWASEQRTLFLVSSKSGSTTEPNAFEAAMA
                     EIAPALDFLAITDPGTALAELARAQEFRGIVEAPPDVGGRYSALTVFGLVPAVLTGVD
                     IGGLLERARAMADACRTDDAVDNPGLRLGAFLGEAALHGRDKLTIVTADRLASFGDWA
                     EQLIAESTGKNGTGIVPIVGEPLADPEAYADDRAFVFLRFADDQDHHARLRARLADLG
                     HPVQELVLNDALDVGGEFVRWEVATAVAGIVLGIDPFDQPNVQESKDATKELLEAYRS
                     KGALPQPMPLVSEPGLAVTADPSALGDEPVSVDGAIGQLLSLLRAGHDYFAILAYLPP
                     DDAVEQQVQRIRTGVRDVLGVATTLGFGPRFLHSTGQLHKGGPPTGVFLQLTAEPSKD
                     LPIPGWDETFGTLIAAQALGDLQSLQRRNRRALRVHMDDLHAGLDRLEAMIDEALAAG
                     STASAATGTKEA"
     gene            complement(3703..4893)
                     /gene="tal"
                     /locus_tag="VFV59_07675"
     CDS             complement(3703..4893)
                     /gene="tal"
                     /locus_tag="VFV59_07675"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012687332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU5325747.1"
                     /translation="MNGAEQDRREAAVTDQDRKAEDVADKANGTNALQRLYAEQNQSP
                     WIDFIDRDLIESGKLEEMVDDGIRGLTSNPTIFAKAVASGQYDDLVRREIEAGDDAHG
                     IYQQIAVSDVGDAADVLRRVYDSSDGADGFASIEVEPELGDDTAATLNRARELWTLLD
                     RPNVFIKIPATEAGIPAIEDAIAEGINVNVTLMFSVEVYRRVARAYVAGLRRRLEAGQ
                     DVSRVASVASFFVSRVDTKVDKALNELGTRAALEARGRAAIANAKLAYEAFNEIFGGD
                     EFAELRAAGARVQRCLWASTSTKNPDYRDVIYVEELIGPETVNTMPLETITAFLDHGD
                     VRRTLDTGLDDARQALREVEASGISMEQVTSELLSEGMASFGKSFDELIESIESKKTE
                     LATA"
     gene            complement(4907..6907)
                     /locus_tag="VFV59_07680"
     CDS             complement(4907..6907)
                     /locus_tag="VFV59_07680"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5325748.1"
                     /translation="MTRPTGPIGPGIGPAWPPSDRHPIPTYHEGVHQRIRQTTCGAFC
                     MTTQHDELRELGQQLRVDSIRSSSAAGSGHPTSSMSAADLMAVLMSKYLHYDFDRPED
                     PRNDHLIFSKGHASPLLYAMYKAAGAISDEEMLTFRQFGSRIEGHPTPVLPWVDVATG
                     SLGQGLPISVGIALTGKRLDRLPYHVWCLCGDSEMAEGSMWEAFEAAGFHELDNLTAI
                     IDVNRLGQTGPTMHQWDLDAYRNRAQAFGWHAIEINGHDVAEVDQAFAEAAQTTGRPT
                     VIVARTEKGHGVAAVANKPGKHGKPLDDPEAAIRELGGMRDIHVDVPKPEINGEPHAF
                     EGGALTLPSYEVGTKEATRKAFGDALLALGKADGRVVAIDGEVGNSTYTEEFAEALPD
                     RFFQMYIAEQQMVSTAVGMQVRGWKPFAATFAAFLSRAYDFVRMSAISRANYALAGSH
                     AGVSIGEDGPSQMALEDLASLRAIIGSTVLYPSDANQAARLVAEMAERQGIVFMRTTR
                     EKTPVLYSPDEEFHIGGSRVVRSSGDDAVTLIGAGITLHEAMKAADALAADGIAARVI
                     DLYSVKPIDAGTVRAAARETGAIVTVEDHSDEGGIGDAVLAALADEQPHPVVRKLAVR
                     EMPGSGKPAELLHFAGIDADCIADAARSLVRGRERVGSAAGE"
     gene            complement(6987..7319)
                     /locus_tag="VFV59_07685"
     CDS             complement(6987..7319)
                     /locus_tag="VFV59_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEU5325749.1"
                     /translation="MTYTDQVITCSDCGIDFVFSASEQEFFAEKGFASAPKRCSSCRA
                     QRRASGGGASSYGNGGGYGGGGGFSRGPREMHDATCARCGNETQVPFRPTGARPVYCS
                     DCFRMMRG"
BASE COUNT         1029 a         2646 c         2536 g         1184 t
ORIGIN      
        1 accttctcac cctcgaccca gccggagacg tactggccgc ggaccgtgtt ggccgccacc
       61 tcctcggggg tcatcggctt cacgccgcgc agcaccttca gcttctcgtc gcgcaggtcc
      121 tcggccgcga actcgaccgg cggctccatg gcgaacatgg ccatgagctg caggccgtgg
      181 ttctggacga tgtcgcgcag cgcccccgtc tggtcgtaga actcgccgcg tccctcgacg
      241 ccgaccgtct cggccaccgt gatctgcacg ctgtcgatat agcggcggtt ccagatcggc
      301 tcgaagaggc cgttgccaaa gcggaagacg gcgaggttct ggaccgtctc cttcccgagg
      361 tagtggtcga tgcgatagac ctgcccctcg tcgaagacct cggcgatctc gtggttcagg
      421 cgccgggcgc tgtcgaggtc gtagccgaac ggcttctcga cgatgacgcg ggtccagcca
      481 cgcttcgcgc cggtgcgccg atgggcgccg ctggcggcga gcccggcccg gtccagctgg
      541 ttgacgatct ccgggtacag gttcggtggg acggcgaggt agaagaggcg gttcccctgc
      601 gtcccgcggt cgcggtcgat gcgatccagc cgtttggcca gctcgacgaa cgccgccgga
      661 tcgtcgaagt cgccgcggtg gtactcgatg cccttcgcga aggagtccca gatcgcgggc
      721 ttcgccgggc ggttgcggct gtacttgttg atgccctcga gcaggtgctc acggaactgc
      781 tcgtcggtca ggtcgcgacg cgcgaagccc acgacggtga actcgggtgg gaggaagccg
      841 ccgagtagcg tgttgtagag cgccggaccc agcttgcgct cggtgagatc accggtggcg
      901 ccgcagatga ccatggtgca cggctccgcc atgcgctcca ggcgcagtcc ctcgcgcagc
      961 gggttggcct tgaccggccg caccgtcgtg atcggcgtcg gctcctggtt cgcggcggct
     1021 cggtcgaccg tgcgcgtcgc catcggtggc tactcgcgct ccttgatggc atggccgccg
     1081 aactcgttgc gaagggcggc aaggacgcga tcggagtagg agtcggggtc gcggcggctg
     1141 cggaagcgct gcagcaggct gagggtgatg accggcgccg gcacgtcatg gtcgatcgcc
     1201 tccgcgacgg tccagcggcc ctcgcccgaa tcggcgaccc agccgcggat ctcgtccagg
     1261 tcgttgccct cgcgctcgaa cgcgttgccg gccagctcga gcagccacga gcggatgacc
     1321 gagccgttgt tccacatgcg ggccacggcc gccaggtcga ggtcgtactc gctggcgtgg
     1381 aggatgtcga agccctcgcc gtaggcctgc atgatcccgt actcgatgcc gttgtggacc
     1441 atcttcacgt agtggccggc cccggcatca ccgcagtaca ggtagccacc ctcgggcgcc
     1501 agcgtcttca ccaccggctc gaaccgatcg aagacctcgc gcttcccgcc cagcatggcg
     1561 cagaagccga cctggaggcc ccagacgccg cccgagacgc ccatgtccag ccactcgatg
     1621 cccttctcgg ccagctcggc ggcgtgacgc ttcgagtcgt ggaagcggct gttgccggcg
     1681 tcgatgatca cgtcgcccgg ctccagcagc tcggccagct gctcgatcat cgcgtcggtc
     1741 gcggcgcccg acgggacgct gacgatgacg tggcgcggtg ccggcagcat ctccaccagc
     1801 cggtcgagcg gctccgcgac gctgacgcgg ccctcgttct cgggctcggc cgccacctcg
     1861 gacgcgacgc tctcggtccg gttccagacc accacgtcgt gcccgccgcg cgccgcgcgg
     1921 cgcgccatgc cggcccccat ccgtcccagt ccgcagatcg cgacctgcat caggcctcct
     1981 tcgtcccggt tgcggcgctc gcggtggagc ccgcggccag cgcctcgtcg atcattgcct
     2041 ccagccgatc gaggccggca tggaggtcgt ccatgtgcac gcgcagggcc cgccggttgc
     2101 gccgctgcag cgattgcagg tcgcccagtg cctgcgcggc gatgagcgtc ccgaaggtct
     2161 cgtcccagcc ggggatcggc aggtccttcg agggctccgc cgtgagctgc aggaagacgc
     2221 cggtgggcgg tccgcccttg tgcagctggc cggtcgagtg caggaagcgc gggccgaagc
     2281 ccagcgtcgt ggccacgccc agcacgtcgc ggacgccggt ccggatgcgc tgcacctgct
     2341 gctcgaccgc gtcgtccggc ggcaggtacg cgaggatcgc gaagtagtcg tgccccgctc
     2401 gcagcagcga gagcagctgc ccgatggcgc cgtccacgct gactggctcg tcccccaacg
     2461 ccgacggatc cgccgtcacc gccagccccg gctcgctcac cagcggcatc ggctgcggca
     2521 gcgcgccctt cgaccgatac gcctcgagca gctccttcgt cgcgtccttg ctctcctgga
     2581 cgttcggctg gtcgaacgga tcgatgccga gcacgatccc ggcgaccgcc gtcgcgacct
     2641 cccagcgcac gaactccccg ccgacgtcca gcgcgtcgtt caagacgagc tcctgcacgg
     2701 ggtggccgag atccgcgagt cgagcacgga gtcgggcatg gtggtcctgg tcatccgcga
     2761 agcgcaggaa gacgaaggcg cggtcgtcgg cgtaggcctc gggatcggcc agcggctcgc
     2821 cgacgatcgg cacgatcccg gtgccgttct tcccggtgct ctccgcgatc agctgctcgg
     2881 cccagtcgcc gaaggacgcg aggcggtcgg ccgtcacgat cgtcagcttg tcgcggccgt
     2941 ggagcgcggc ctcgcccagg aaggcgccga gccgcaggcc cgggttgtcg accgcatcat
     3001 cagtccggca ggcgtccgcc atggcgcgcg cacgctccag gagcccaccg atgtcgacgc
     3061 cggtcagcac agccggcacc aggccgaaga cggtcagcgc tgagtagcgg ccgcccacgt
     3121 cgggcggcgc ctcgacgatg ccgcggaact cctgggcgcg ggccagttcg gccagcgcgg
     3181 tgccgggatc ggtgatagcg aggaagtcga gggccggcgc gatctcggcc atggcggcct
     3241 cgaaggcatt cggctcggtg gtggagccgg acttcgagct caccaggaag agcgtgcgct
     3301 gctccgatgc ccacgagcgg aagccgcgca ccacgtcggg atgcgtcgag tccagaatgc
     3361 gcagctcgag cccatcggcc gacgcggcgc cggtgcccgc cgggcctccg gcccagccga
     3421 agacccggct gaacagctcg ggcgccaggc tcgagccacc catcccgagc accgcggcgc
     3481 gacggtagcc gtcctcgcgc acggcgcggg caaggtgctc gagctcggtg acgcgctccg
     3541 acatcgcgtc cggcagcgcc agccatccga gccatgccgc cacctcgtcc ggcttcttct
     3601 gcgactccgc ccacagcgag ccgtcgcgcg cccagaggcg ctcggccacc cgttcgccgg
     3661 tgaggcgctc gaggcgggcc tcgacggcgt cctcgacgcc ggtcatgcgg tcgccagctc
     3721 cgtcttcttc gactcgatcg actcgatcag ctcgtcgaag ctcttcccga aggacgccat
     3781 gccctcgctg agcagctcgg acgtgacctg ctccatgctg atgccgctgg cctccacctc
     3841 gcgcagcgcc tgtcgcgcgt cgtcgaggcc ggtgtccagc gtgcgacgca cgtcgccgtg
     3901 gtccaggaag gccgtgatgg tctccaacgg catggtgttc acggtttcgg gaccgatgag
     3961 ctcctccacg tagatcacgt cgcggtagtc ggggttcttc gtgctggtac tcgcccacag
     4021 gcagcgctgc acgcgggcgc cggcggcgcg cagctcggcg aactcgtctc cgccgaagat
     4081 ctcgttgaag gcctcgtacg ccagcttcgc gttcgcgatg gcggcacgtc cgcgggcctc
     4141 gagcgccgcg cgggtgccga gctcgttcag ggccttgtcc accttcgtgt cgacgcggct
     4201 gacgaagaag ctcgcgacgc tcgcaacgcg gctcacgtcc tgccccgcct cgagccgcct
     4261 ccgcaggccg gccacgtagg cgcgcgccac gcggcggtag acctcgacgc tgaacatcag
     4321 cgtcacgttg acgttgatgc cctcggcgat ggcgtcctcg atggccggga tgccagcctc
     4381 cgtcgcaggg atcttgatga agacgttggg ccgatcgagg agcgtccaca gctcgcgggc
     4441 gcggttgagc gtggccgcgg tatcgtcgcc gagctccggc tcgacctcga tgctggcgaa
     4501 gccatcggcg ccgtccgagc tgtcgtagac ccggcgcagc acatcggcgg cgtcgccgac
     4561 gtcgctgacc gcgatctgct ggtagatacc gtgcgcgtcg tcgcccgcct cgatctcgcg
     4621 ccggaccagg tcgtcgtact ggccgctggc gacggccttg gcgaagatcg tcgggttgct
     4681 ggtgagcccg cggatgccgt cgtcgaccat ctcctcgagc ttgccgctct cgatcaggtc
     4741 acggtcgatg aagtcgatcc acgggctctg gttctgctcg gcgtacagcc gctgcagcgc
     4801 gttggttccg ttggccttgt ccgccacgtc ttccgccttc ctgtcctggt cggtcactgc
     4861 cgcctcccgg cggtcctgct ctgctccgtt catcgcgcgc gcctccctac tcgccggcgg
     4921 ccgagccgac gcgctcgcgg ccgcgaacga gcgaccgtgc cgcatcggcg atgcagtccg
     4981 cgtcgatccc ggcaaagtgg agcagctcgg cgggcttgcc cgagcccggc atctcgcgca
     5041 cggccagctt ccggaccacc gggtgtggct gctcgtcggc gagcgcggcg aggaccgcgt
     5101 ccccgatgcc gccctcgtcc gagtgatcct ccacggtgac gatcgcgccc gtctcccgcg
     5161 ccgcggcgcg aaccgtcccg gcatcgatcg gcttcacgct gtacaggtcg atcacgcgcg
     5221 ccgcgatgcc gtcggcagcg agcgcatcgg cagccttcat ggcctcgtgc agggtgattc
     5281 cggcaccgat cagcgtcacc gcgtcgtcgc cggaactgcg caccacccgc gatccgccga
     5341 tgtggaactc ctcgtcgggc gagtacagga ccggcgtctt ctcgcgcgtc gttcgcatga
     5401 acacgatgcc ctggcgctcc gccatctcgg ccacgagcct ggccgcctgg ttggcgtcac
     5461 tggggtacag caccgttgat ccgatgatcg cccgcaggct ggcgaggtcc tcgagcgcca
     5521 tctgcgacgg tccgtcctcg ccgatgctga cgccggcatg cgaccccgcc agcgcgtagt
     5581 tcgcccggct gatggccgac atccgcacga agtcgtacgc acgcgacagg aaggccgcga
     5641 acgtcgcggc gaacggcttc cagccacgca cctgcatgcc gaccgccgtc gacaccatct
     5701 gctgctcggc gatgtacatc tggaagaacc gatcgggcag cgcctcggcg aactcctccg
     5761 tgtaggtgct gttgccgacc tcgccgtcga tggcgacgac gcgcccatcg gccttgccca
     5821 gcgccagcag cgcatcaccg aaggccttgc gcgttgcctc cttcgtgccg acctcgtatg
     5881 acggcagggt cagggcacca ccctcgaagg cgtgcggctc accgttgatc tctggcttcg
     5941 ggacatcgac gtggatgtcg cgcatcccgc ccagctctcg gatcgctgcc tcagggtcgt
     6001 cgagcggctt gccgtgcttc cccggcttgt tcgcgaccgc ggcgacgccg tgccccttct
     6061 cagtcctcgc gacgatgacc gtcgggcgcc cggtcgtctg cgccgcctcg gcgaaggcct
     6121 ggtccacctc cgcgacgtca tggccgttga tctcgatggc gtgccagccg aacgcctgcg
     6181 cccggttgcg gtacgcgtcg aggtcccact ggtgcatggt cggcccggtc tgacccagcc
     6241 ggttcacgtc gatgatggcg gtcaggttgt ccagctcgtg gaagccggcg gcctcgaatg
     6301 cctcccacat ggagccctcg gccatctccg agtcgccgca caggcaccac acgtggtacg
     6361 ggagccgatc caggcgcttc ccggtgagcg cgatcccgac gctgatgggc aggccctggc
     6421 ccagcgatcc ggtggcaacg tccacccagg gaagaaccgg cgtcgggtga ccctcgatgc
     6481 ggctgccgaa ctgccggaag gtgagcatct cctcgtcgga gatggccccg gccgccttgt
     6541 acatggcgta gaggagcggc gacgcgtgcc ccttgctgaa gatcaggtgg tcgttgcgtg
     6601 gatcctcggg tcgatcgaag tcgtagtgca ggtacttcga catgaggacg gccatgaggt
     6661 cggccgcgga catggaggag gtcgggtgcc cgctgccggc ggcgctcgag gatcgaatcg
     6721 agtcgacgcg cagctgctgc ccgagctcgc gcaactcgtc gtgctgggtg gtcatgcaga
     6781 acgctccgca agttgtctgc cgaattcgtt ggtgaacacc ctcatgatag gtcgggattg
     6841 ggtgacggtc gctcggcggc caagccgggc cgattccggg cccgattgga cccgtggggc
     6901 gggtcatggg tggcggagtt ccgcggggca agaagaacgg gcggcccgga ggccgcccgt
     6961 atcggctgcg aagggagaga cgaggactag ccccgcatca tccggaagca gtcggagcag
     7021 tagaccgggc gcgcgcccgt cgggcggaac ggcacctggg tctcgttgcc gcagcgcgcg
     7081 caggtcgcgt cgtgcatctc gcgcggcccg cgcgagaacc cgccgccgcc gccgtagccg
     7141 ccgccgttgc cgtagctgct ggcaccgccg ccggatgccc ggcgctgggc gcggcacgag
     7201 gagcagcgct tcggagcgga cgcgaagccc ttctccgcga agaactcctg ctcggatgcg
     7261 gagaacacga agtcgatccc gcagtcggaa caggtgatca cctgatcggt gtaggtcact
     7321 gacggacccc tcccaacaat gagttgaacc gcgtcggttc cgtcgctcga cgccggccgg
     7381 ggatcttgcc gctgc
//
LOCUS       DASTYP010000168        13292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_195372, whole genome shotgun sequence.
ACCESSION   DASTYP010000168 DASTYP010000000
VERSION     DASTYP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13292
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195372"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFV59_07690"
     CDS             <1..307
                     /locus_tag="VFV59_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325750.1"
                     /translation="LRVVGGTAFIDDVSATAAGPGVKRSFLVKFSPQLDNLPDLRALD
                     TKLLVAGEGGDDEISVQSIDQPTYVLGGSVLGADAPAVDDDDHPRVERTDASAGRRA"
     gene            complement(552..1340)
                     /locus_tag="VFV59_07695"
     CDS             complement(552..1340)
                     /locus_tag="VFV59_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEU5325751.1"
                     /translation="MTAADAVSRGRFHEAPRSGEARGRADMHIHSLYSDGTASVAQIL
                     EHVEHHTDLDVIAITDHERVDGALRAAELHAAGSFTFDLVVGEEITTRRGHVVALFIT
                     ERIAALRPLEETLDAIHAQGGIAIAAHPMAPVPLSVGRRSLLGVRDHEHERVYFDALE
                     LFNPSHAGRMRQDARLELNAAELGLPGVGNSDGHVLEGIGTGYTRFPGHTAEEYREAV
                     RAGTVEPGGEHWTAAHNVDVYRRQLHAKLRHVWHTVKPGHDDWR"
     gene            complement(1337..2227)
                     /locus_tag="VFV59_07700"
     CDS             complement(1337..2227)
                     /locus_tag="VFV59_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325752.1"
                     /translation="MLLRLGLEAADRLANLLPARVAYALADVAGDAWRRLAPRRRELV
                     AANLRRVCEASGRPTAGPTFRRLVRDAFRHHARYYLEVLRVPHVPTARIDEIVDVPQW
                     PEFARAIDGRPSIMVSWHLGNPEPFSTFLASRGIRPLVPIEEIEPRALFEFLAARRGA
                     AHPELVPVRRSRRPLTQRLRDGGLVAIIGDRDLDHDGRPVTVFGHPTTLPTGPAWLAA
                     THRAAILVGRCLREGEGHYRVDGEMIDAGAMEGDRRAMVAELTERLARRFEADVAAAP
                     EQWWGAFQPFWPDLANRDER"
     gene            complement(2227..3219)
                     /locus_tag="VFV59_07705"
     CDS             complement(2227..3219)
                     /locus_tag="VFV59_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5325753.1"
                     /translation="MTDSAAAARAGSEKTPERKRELVTYWLYRAGEWAINRLPRGFVL
                     PAAATVGALGYLLAGPKRALIRRNLARPMQLPADDRRVDRAARQAFANYAKYLVDMMR
                     IGEMTEEQAEELVGIEGLEVLRAAQAEGRGVLICTVHVGGMDLIGPGLKLHGEALNVV
                     ADDTTYGRLYDHLAAARARQHIFLIGWRNLRGLYRTLRGNGNLVLFCDGGYRRGDVPV
                     EFCGEATTFPIGPATLAAKTGAPILPVACRRLPNDRFVARALPLIHCTSTEPTELYRA
                     TQELAHALGGVLAEVPGQWYMFRPVWPEAAADRERAEAALAAARRGEDWTTIGA"
     gene            complement(3242..4474)
                     /locus_tag="VFV59_07710"
     CDS             complement(3242..4474)
                     /locus_tag="VFV59_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU5325754.1"
                     /translation="MASTNGNGSAAKNGHTKGDGKVRVAIVGVGNCASSLVQGRYYYA
                     DAKDGDFVPGLMHVNLGGYHVRDIEFVAAFDIDKNKVGKDLSEAIYAEPNNTYRFADV
                     PHTGVTVERGMTHDGLGKYLSQVIEKAPGPTADIVGILKERKVDVLVSYLPVGSEQAT
                     KWYAEQAIQAGVAFVNAIPVFIGREPYWQRRFAEAGLPVIGDDIKSQVGATITHRVLT
                     RLFMDRGVRIDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSMIDYDIADDDVH
                     VGPSDYVPWLKDRKWCHIRMEGTTYGDVPLNVELKLEVWDSPNSAGVITDAIRCAKLG
                     LDRGLAGTLVAPSSYFMKSPPIQIHDDIAHNRVEAFIRGEDNETLVGTEQPPKRARKH
                     LAPGHANVPAKAAAGEAR"
     gene            complement(4480..5157)
                     /locus_tag="VFV59_07715"
     CDS             complement(4480..5157)
                     /locus_tag="VFV59_07715"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5325755.1"
                     /translation="MSASGPRTRPLRQGSVLPPWVKEGGRAVLAPVVRTAMALHLTPN
                     TITVIGLLITLVASALVAGGWLLLGAAILTAGSLLDAVDGALARATGGGTAFGSFLDS
                     TLDRAGETILYLGVTAWLLRAIDQPFVPVLLALMALGGSFLVSYAHARAQGIGLEASV
                     GLAPRTERLVLVIAGIALAGLGFSIGLIGALAILAILTVITVVQRIWHVWRLSQPAPT
                     APEHKEN"
     gene            complement(5147..5509)
                     /locus_tag="VFV59_07720"
     CDS             complement(5147..5509)
                     /locus_tag="VFV59_07720"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5325756.1"
                     /translation="MISPQPESPAEHDLRRLRVLYRALGDETRLRVIGLLAEVGPMPV
                     RELSSRVGLSQPLISWHLRILRLAGLIDTRRQGREVICRLRTAAFEELHDAEARLIGG
                     TAGVAQASEAPGTVPDVS"
     gene            5618..6427
                     /locus_tag="VFV59_07725"
     CDS             5618..6427
                     /locus_tag="VFV59_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5325757.1"
                     /translation="MALTSSTPDALWADPAVRVLRPAALRATDVRRGRAIDGCSLAVP
                     VGMRLLLVSEPDGPASTLVRILAGLARPSRGRVEIAGLRDASPGGWGRRVAYLGPEPG
                     IRPWMTPREALVLAADLLELEGEEAERRIRRALAWTRIDAGSADRPVRRGGLPLLQRT
                     GLAASLVGDPELLLLDEPLRAIDAEERRRMLRLPGRRRTIVLASRYPASEEGLVSHVA
                     LLRGGRIALVAPVEALGQAGLPLSGRGIATLAELRDRIRGAPPAVRAPAAR"
     gene            6424..7089
                     /locus_tag="VFV59_07730"
     CDS             6424..7089
                     /locus_tag="VFV59_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325758.1"
                     /translation="MSQSTAIARLALRELWISFRLLVLLVAFVAAGTLVALVPAPFPA
                     QADRLVLGVTAASALAGALGAWSLAVERRSGRAGWLVSRSVPRGTLLGGWFVGIAAST
                     LAGLATTLALGWLALPVGAVTDVARFGLVAAVLAAAAISWVSLGLLAGTVAAPALASL
                     SVLVAGAAVLAARSSGVLPDAAGWLFVHAAAGELLRLIGALLALAGVLLATAHVAMGR
                     VEL"
     gene            7086..8663
                     /locus_tag="VFV59_07735"
     CDS             7086..8663
                     /locus_tag="VFV59_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325759.1"
                     /translation="MTAVPEDAPPRLHPVAAWAALLFASLGVLLGGAELMVVAIALPS
                     IVADFGGWNDLARVSWIVNAYLLAYVVAMPLAGRGADLWGAMRLYVVALLLFVVGSLG
                     SGLSRLAGPDDGLTWLIGWRVVQGFGGGALVPLSMALASHLFRGRARSTALGVEGAAT
                     YVGMAIGPVYGAWVLQNFHLPLPGIDMASWQWIFLLNVPIGIVTLLLIYVAAGGIETP
                     RVRARVDLGGAVLLSAALVAGIGAVTVSGAQGWTDPLVIAGLVASALAFAAFTWHQLR
                     SRSPLVDVRLFADRAFSAANGVSLLTGYTLATAVIGGPVFVNRVLFGTNEQSSAALTA
                     LTLAIAAAALVGGVVSGFVGERLVTLVGVVVSAIGLWLSLGWGVETGLDELVRDLAIF
                     GIGFGLTVSPRATAAVEAAGSGAYGVASAMLQITRTVGMSIGLALLTSIGQNRIDELS
                     QLVNDPARRDELVRTLGRPEFVGVDPQASLALVDLLENWSRSQAAGVLQLVFAIALGV
                     AVVTLLPAWFVSGRRRA"
     gene            complement(8688..9443)
                     /locus_tag="VFV59_07740"
     CDS             complement(8688..9443)
                     /locus_tag="VFV59_07740"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEU5325760.1"
                     /translation="MKRLSGWWLPIILGVAGVALIIAGSLDSGGPPGPTLPPIAEVTA
                     TPSASARATSSATLEPTREPSASAAATATPTPTPLPDDVVAVRLEVPSVGIRVNVFAP
                     TDASHCAFPPSDGAYLICRTDESGNLQAPDQPGHNTNAYVFAHAVNDLFKPLWNVQVG
                     AEVLVLMSNEQVLRYVVTEVRPNVACPDPDATTNNPEDFGITPPLALQVHDDCDEGVF
                     WTQPTDHERLTLQTSQGYNRNWGELVVVADPEF"
     gene            9444..10322
                     /gene="otsB"
                     /locus_tag="VFV59_07745"
     CDS             9444..10322
                     /gene="otsB"
                     /locus_tag="VFV59_07745"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU5325761.1"
                     /translation="MTESSRRPTPSPTGSPTGQPTASPLAPPSASLSEALELARDVLS
                     ARPAGVLTDFDGTLSPIVTDPALARLVEGGHGALARLAEQLEVVAIITGRAALDARRM
                     TGVPGLLIAGNHGTEWLEPTADEPVAAPGMEDVRSRLDRALARLPELEGVPIEHKGLS
                     AAIHYRNAPDPAATRRAILDGLGDVGGEGLELRHGRMTVELRAIGAADKGTATRALVE
                     RHALRAVVVLGDDVTDLDMFRAVIALRAEGRVRGLVVGVGGGDHEVPEEVRGAADVLL
                     RDPAEAAAFLEGLTRV"
     gene            complement(10335..11678)
                     /locus_tag="VFV59_07750"
     CDS             complement(10335..11678)
                     /locus_tag="VFV59_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5325762.1"
                     /translation="MVQPPAPRRRWGVPDLVRIGRFQRDARVFLVSTLVLGAALSLYW
                     IDFNLYLRSLGFSTATVGLVATISSTAGAVTALPASALSDRIGRRAVMAGGVIVGVLA
                     LIGLLSVEALPLIIGFAALWAIGQQSLMVVQAPFLAEHSDAEHRNDLFALQAAIQTVT
                     NVVAAALGGIVATAIAGAMGSDPDGPTTYRVILVIMVGLLMVGLVTIGLLGDDRPSRM
                     RSAARLKAIGEPAAFPPQRATSPARMGIVIRDRRRFVRLLLPGFLIAIGAGQVIPFLN
                     VFVQQKFGLELASLNALFAFTSLGTVAAMLAQPAMARRFGQITSVVIVQAFSIPFLVV
                     LGFSPILWTVVLAMVVRNSLMNAGNPIFSAFAMEHVSPAERATLSASMSLLWQIGWVV
                     GGGWYALLQATLGFTGGYAVNFLTVISLYSIATLLYWAWFGAADRRAAAERRLAA"
     gene            complement(11686..13191)
                     /locus_tag="VFV59_07755"
     CDS             complement(11686..13191)
                     /locus_tag="VFV59_07755"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU5325763.1"
                     /translation="MPHDADSALGAAASARLGDRRLIIATNRGPVTFTTAADGGLRPR
                     RGSGGLVTALGQVGRHVPVTWVAAAMGDGDRRAAADPKLLERAAAVDGQDVRLRFATV
                     ERNVYEQAYNVIANPFLWFLQHQMWNLPERPVIDAALMRAWERGYVALNEAFASAVLA
                     QVRGDREPRIMLHDYHLYLAAEPIRRARPKAVLSHFTHIPWPPSSIWLTIAPTIRESI
                     ATGLLANDVVGFQTARYAHNFLRMVESFVPDAKVDYESSTVRRGRRTTHVRSYPISID
                     VAATRRVAHSRAARRRADDLRGRTREATLVRVDRLEPSKNILRGFLAFESLLQRNPRL
                     RNRIVFFAFLVPSRTGLREYGYYGRQVQNAVDRINARFGNAEWRPVQLFYENDYAQAL
                     AGLSIADVVLVNPLVDGMNLVAKEAVVVSQHDSVLVLSETAGAFDQMADGALAVAPAD
                     VVGTADAIATALAMEPDERRRRLRLLRAGVEREDITWWLRRQLEDLADIAG"
BASE COUNT         1727 a         4604 c         5047 g         1914 t
ORIGIN      
        1 cctgcgggtg gtcggcggca cggccttcat cgacgacgtg agcgccaccg cggccggacc
       61 gggcgtgaag cgcagcttcc tggtgaagtt ctccccgcag ctcgacaacc ttccggacct
      121 gcgcgccctg gataccaagc tgctggtcgc gggcgagggc ggcgacgacg agatcagcgt
      181 gcagtcgatc gaccagccga cctacgtgct gggcggcagc gtcctcggcg ccgacgcgcc
      241 ggcggtggac gacgacgacc atcccagggt cgaacggacc gatgcatcag cgggaaggcg
      301 agcgtgagcg cgccgccgcc gaagacgaag ttccggacgg gaatgccctt cccggcgaag
      361 cgggggtcgt ccgccggcgc ggccgccgcc tcgacgacga agccgacgat ggcggcgcga
      421 agggcgccgt tgaccaccca gccggccacg cgccggcgac gatcggaccc ggccatgccc
      481 ggatggtccc gctcgcgtgc gcggccggcg agggccgaat gggccgttcg gatcggtcct
      541 cgcggatcgg ctcagcgcca gtcgtcgtgg ccgggcttga ccgtgtgcca gacgtgccgc
      601 agcttggcgt gcagctgccg gcggtagacg tcgacgttgt gcgccgcggt ccagtgctcc
      661 ccgccgggct cgaccgtgcc ggcgcggacg gcttcgcggt actcctcggc ggtgtggccc
      721 gggaagcgcg tgtagccggt gccgatgccc tcgaggacgt ggccgtcgga gttgcccacg
      781 ccgggcagac ccagctccgc ggcgttcagc tccagccgcg cgtcctggcg catgcgtccg
      841 gcatgactcg ggttgaacag ctcgagggca tcgaagtaga cgcgctcgtg ctcgtggtcg
      901 cgcaccccga gcaggctgcg gcggccgacg ctcagtggca cgggcgccat cggatgggcc
      961 gcgatggcga tgcccccctg cgcgtggatg gcgtcgagcg tctcctcgag cgggcgcagc
     1021 gccgcgatcc gctcggtgat gaacagcgcg acgacgtggc cgcgccgcgt cgtgatctcc
     1081 tcgcccacga ccaggtcgaa ggtgaagctc ccggccgcgt gcaactccgc ggcgcgcagc
     1141 gcgccgtcga cgcgttcgtg gtcggtgatg gcgatcacgt cgagatcggt gtggtgctcc
     1201 acgtgctcga ggatctgcgc gaccgatgct gtcccgtccg agtacagcga gtggatgtgc
     1261 atgtcggcgc ggccgcgcgc ctcaccgctg cgcggtgcct cgtggaagcg gccccggctg
     1321 accgcgtcgg cggcggtcac cgctcgtctc ggttcgccag gtccggccag aacggctgga
     1381 acgcccccca ccactgctcg ggggccgcgg cgacgtcggc ctcgaaacgc ctcgccaggc
     1441 gttcggtgag ctccgcgacc atggcccgac ggtcgccttc catcgccccg gcgtcgatca
     1501 tctcgccatc gacgcggtag tgtccctcgc cctcgcgcag acagcgcccg accaggatcg
     1561 ccgcgcgatg cgtggccgcc agccaggccg ggccggtcgg caacgtcgtg gggtggccga
     1621 agacggtgac cggccggccg tcgtgatcga ggtcacggtc gccgatgatc gcgaccagtc
     1681 cgccgtcgcg cagccgctgc gtcagcggcc gcctcgagcg gcgcaccggc acgagctcag
     1741 ggtgggccgc accgcgacga gcggccagga actcgaacag cgcgcgcggc tcgatctcct
     1801 cgatgggcac cagtggccgg atcccgcgcg acgccaggaa ggtggagaac ggctcggggt
     1861 tgccgaggtg ccagctgacc atgatcgacg gccgaccgtc gatggcgcgc gcgaactcgg
     1921 gccactgcgg gacgtcgacg atctcgtcga tccgggcggt cggtacgtgc ggaacacgca
     1981 gaacctcgag gtagtagcgc gcgtggtggc ggaacgcgtc gcggaccagg cggcggaagg
     2041 tcgggcccgc cgtgggccgg ccggaggcct cgcacacgcg gcgcaggttg gcggccacca
     2101 actcgcgccg ccggggagcc aggcgccgcc aggcgtcacc ggccacgtcg gccagggcgt
     2161 aggcaacgcg cgcgggcagc aggttggcga ggcggtcggc ggcctcgagt cccaggcgga
     2221 ggagcatcac gccccgattg tcgtccagtc ctcgccgcga cgcgctgcgg cgagggccgc
     2281 ctcggcccgc tcccggtccg cggccgcctc gggccacacg gggcggaaca tgtaccactg
     2341 ccccggtacc tcggccagca ccccgcccag ggcgtgcgcc agctcctgcg tcgcgcggta
     2401 cagctcggtc ggctcggtgg aggtgcagtg gatgagcggc agggcgcgcg ccacgaatcg
     2461 gtcgttcggc aggcggcggc aggcgaccgg caggatcggc gcgccggtct tcgcggccaa
     2521 cgtcgcgggt ccgatcggga aggtggtggc ctcgccgcag aactcgaccg ggacgtcgcc
     2581 gcggcggtag ccaccgtcgc agaacagcac caggttgccg ttgccccgca gcgtgcggta
     2641 caggccgcgc aggttgcgcc acccgatgag gaagatgtgc tggcgagcgc gcgccgcggc
     2701 caaatggtcg tagaggcggc cgtaggtggt gtcatccgcc accacgttca gggcttcgcc
     2761 gtgcagcttc aggccggggc cgatgaggtc catgccgccg acgtgcacgg tgcagatgag
     2821 gacgccgcga ccctccgcct gtgcggcgcg cagcacctcc agcccttcga tgccgaccag
     2881 ctcctcggcc tgctcctccg tcatctcgcc gatgcgcatc atgtcgacca ggtacttcgc
     2941 gtagttcgcg aaggcctgcc gcgccgcgcg gtccacgcgc cgatcgtcgg cgggcagctg
     3001 catgggccgg gcgagattcc ggcggatcag cgctcgcttg ggccccgcga gcaggtagcc
     3061 gagcgcgccg accgtggctg ccgcggggag cacgaagccg cgcggcagcc ggttgatggc
     3121 ccactcaccg gcgcggtaca gccagtaggt gaccagctcg cgcttgcgct cgggcgtctt
     3181 ctccgagccg gcccgcgccg ccgccgcgct gtccgtcatg cgttcggccg cgagggacga
     3241 atcaccgcgc ctcgccggca gcggccttgg ccggcacgtt ggcgtggccg ggcgcgagat
     3301 gcttcctggc acgcttcggc ggctgctccg tgccgaccag cgtctcgttg tcctcgccgc
     3361 ggatgaaggc ctcgacgcgg ttgtgggcga tgtcgtcgtg gatctggatc ggcgggctct
     3421 tcatgaagta ggagctcggc gcaaccagcg tgccggccag gccccggtcc aggccgagct
     3481 tggcgcagcg gatggcatcg gtgatgacgc cggcgctgtt gggggagtcc cacacctcga
     3541 gcttgagctc cacgttgagc ggcacgtcgc cgtaggtggt gccctccatc cggatgtggc
     3601 accacttgcg gtccttcagc catggcacgt aatcggaggg gccgacgtgg acgtcgtcat
     3661 cggcgatgtc gtagtcgatc atgctggtga ccgcgttggt cttgctgatc ttcttcgact
     3721 cgaggcgctc gcgctccagc atgttgagga agtcggtgtt gccgccgaag ttcagctgat
     3781 aggtgcgatc gatccgcacg ccgcggtcca tgaagaggcg ggtcaacacg cggtgggtga
     3841 tggtcgcgcc cacctggctc ttgatgtcgt cgccgatgac gggcaggccc gcctccgcga
     3901 agcggcgctg ccagtacggc tcgcgcccga tgaagacggg gatggcgttg acgaaggcga
     3961 cgcccgcctg gatcgcctgc tcggcgtacc acttggtcgc ctgctcgctg ccgacgggca
     4021 ggtagctgac caggacgtcg accttgcgct ccttgaggat gccaacgatg tcggccgtgg
     4081 ggccgggcgc cttctcgatg acctggctca ggtacttgcc caggccgtcg tgggtcatgc
     4141 cgcgctcgac ggtgacgccc gtgtgcggca cgtcggcgaa gcggtaggtg ttgttgggct
     4201 cggcgtagat cgcctcggag aggtccttgc cgaccttgtt cttgtcgatg tcgaaggccg
     4261 ccacgaactc gatgtcgcgc acgtggtagc cgccgaggtt gacgtgcatg aggccgggga
     4321 cgaaatcgcc gtccttcgca tcggcgtagt agtagcggcc ctgcaccagg ctgctggcgc
     4381 agttgccgac gccgacgatg gcgacccgca ccttgccgtc ccccttggtg tggccgttct
     4441 tggcggccga gccgttgccg ttcgtggatg ccacgggtgt cagttctcct tgtgctcggg
     4501 tgcggtgggg gcgggctggc tgagacgcca gacgtgccag atgcgctgga cgacggtgat
     4561 gaccgtgagg atggccagga tggccagggc cccgatgagg ccgatgctga agccgaggcc
     4621 ggcgagggcg atgccggcga tgacgaggac gaggcgctcg gtccgcggtg ccaggccgac
     4681 gctcgcctcg agtccgatgc cctgggcgcg ggcgtgcgcg tagctcacca ggaacgagcc
     4741 gcccagggcc atcagcgcga gcaggacggg cacgaagggc tggtcgatgg cccgcagcag
     4801 ccaggccgtc acgcccaggt agaggatcgt ctcgccggcg cggtcgaggg tcgagtcgag
     4861 gaagctgccg aacgcggtgc cgccgccggt ggcgcgcgcc agggcaccgt ccacggcatc
     4921 cagcagcgac ccggcggtca ggatggccgc gcccaggagc agccagccgc cggcgacgag
     4981 ggccgaagcg acgagcgtga tgagcaggcc gatgacggtg atcgtgttgg gcgtgagatg
     5041 cagcgccatg gcggtccgca ccacgggcgc gagcaccgcg cggccgccct ccttgaccca
     5101 cggcggcagg accgatccct gccgcagcgg ccgcgtccgc gggccgctag ctgacatccg
     5161 gcaccgtccc cggggcctcg gacgcttggg cgacgccggc cgtgccgccg atgaggcgtg
     5221 cctcggcgtc gtgcagctcc tcgaacgcgg ccgtgcgcag gcgacagatg acctcccggc
     5281 cctggcgtcg cgtgtcgatc agcccggcca ggcggaggat gcggaggtgc cagctgatca
     5341 agggctgcga gagtccgacg cggctcgaga gctcgcgcac cggcatggga ccgacctcgg
     5401 ccagcaggcc gatgacgcgc agccgtgtct cgtcgccgag ggcgcggtag aggacgcgca
     5461 gacgacgcag atcgtgctcg gccggggact ccggctgggg ggagatcaca gagcgcttct
     5521 ttatcaaatg cggatttata taagcggatg cttatcctat gcccgggcgg ccgaggcgtc
     5581 aaggctgcgt gctaggatcg gccggcgaga tggaggtgtg gcgctgacaa gctcgacccc
     5641 ggacgccctc tgggccgatc cggcggtgcg cgtcctgcgc ccggccgcgc tgcgagccac
     5701 ggatgtccgc cgtggccgcg ccatcgatgg ctgctccctg gccgtcccgg tcgggatgcg
     5761 gctgctgctg gtgtccgagc ccgacggccc ggcgtccacg ctggtgcgga tcctggccgg
     5821 cctcgcgcga ccgtcgcggg gacgggtcga gatcgccggc ctccgagatg cctctcccgg
     5881 cggatggggc cgacgggtcg cgtacctggg cccggagccc ggcattcgtc cgtggatgac
     5941 gccgcgagag gcgctcgtgc tggccgccga cctattggag ctggagggcg aggaagccga
     6001 acggcggatc cggcgggcgc tggcatggac gcgcatcgac gctgggtcgg cggaccggcc
     6061 cgtgcgtcgg ggcgggctgc cgctcctgca gcgcaccggc ctcgccgcct ccctggtcgg
     6121 tgatcccgag ctgctgctgc tcgacgagcc gctgcgcgcc atcgacgcgg aggagcgtcg
     6181 acgcatgctt cgcctgccgg ggcggcgccg gaccatcgtg ctcgcgagcc gctacccggc
     6241 cagcgaggag ggcctggtct cgcacgtcgc tctgctgcgc ggcggacgga tcgcgctggt
     6301 ggcgccggtg gaggcgctcg ggcaggccgg gctgccgctg tcgggtcggg gcatcgcgac
     6361 gctcgcggag cttcgggatc ggattcgagg cgcacccccg gcggtgcggg cgcccgccgc
     6421 gcgatgagcc agtcgaccgc gatcgcccgg ctcgcgcttc gcgagctgtg gatcagcttt
     6481 cggctgctcg tcctgctcgt cgcgttcgtc gcggccggga cgctggtcgc cctcgttccg
     6541 gcgcccttcc cggctcaggc cgaccggctc gtcctcggcg tcacggcggc atcggccctg
     6601 gccggtgcgc tgggcgcctg gtcgctggcg gtggagcgcc gatcgggccg ggccggctgg
     6661 ctggtgtcgc gctccgtccc gcgcggcacg ctcctgggcg ggtggttcgt cgggatcgcg
     6721 gcatccacgc tggccggcct ggcgacgacc ctcgccctgg gctggctggc cctgccggtg
     6781 ggcgcggtga ccgacgtggc gaggttcggc ctggttgccg ccgtcctggc ggccgcggcc
     6841 atcagctggg tgtcgctcgg gctcctggcc ggcacggtgg ccgccccggc cctggcatcg
     6901 ttgagcgtcc tcgtcgccgg cgcggcggtg ctggcggccc ggagcagcgg cgtcctgccc
     6961 gacgccgccg gctggctctt cgtgcacgcg gcggcggggg agctgctgcg gttgatcggc
     7021 gcgctcctgg cgctggcagg cgtcctgctt gcgacggcgc acgtggcgat gggtcgcgtc
     7081 gagctgtgac ggcggtgccg gaggacgcgc cgccgcgact gcatccggtc gcggcgtggg
     7141 cggcgctgct cttcgccagc ctgggcgtgc tcctgggcgg cgccgagctg atggtggtag
     7201 ccatcgccct gccgtcgatc gtggccgact tcgggggctg gaacgacctg gctcgcgtca
     7261 gctggatcgt caatgcgtac ctgctggcct acgtcgtcgc catgcccctg gccggccggg
     7321 gcgccgacct gtggggcgcc atgcggctct acgtggtggc gctcctgctc ttcgtggtcg
     7381 gcagtctggg ctcgggactg tcgcggctgg cagggccgga cgacggcctg acctggctga
     7441 tcggctggcg cgtggtgcag ggctttggtg gcggcgcgct cgttccgctg tcgatggcgc
     7501 tggccagtca cctgttccgc ggtcgggcgc gctcgacggc gctcggggtc gagggggcgg
     7561 ccacgtacgt cggcatggcg atcgggccgg tgtacggggc ctgggtgctg cagaacttcc
     7621 acctgccgct gcccggcatc gacatggcca gctggcagtg gatcttcctg ctcaacgtgc
     7681 cgatcggcat cgtgaccctg ctgttgatct acgtcgcggc cggcgggatc gagacgcctc
     7741 gcgtgcgcgc gcgcgtcgac ctcggtggcg cggtgctgct gtcggcggcg ctggtggccg
     7801 gtatcggtgc ggtgaccgtc tcgggcgcgc agggctggac ggatccgctc gtcatcgctg
     7861 ggctggtggc cagcgcgctc gcgttcgcgg cgttcacctg gcaccagctg cggagccggt
     7921 cgccgctggt cgacgtccgc ctcttcgccg atcgggcgtt cagcgcggcc aacggcgtca
     7981 gcctgctgac cggctacacg ctggcgacgg cggtcatcgg cgggcccgtc ttcgtcaacc
     8041 gggtgctgtt cggcacgaac gagcagtcgt ccgcggcgct gacggcgctc accctggcca
     8101 tcgccgcggc ggccctcgtc ggcggggtcg tctcgggttt cgtgggcgag cggctcgtca
     8161 cgctcgtcgg cgtggtggtc agcgccatcg gcctgtggct gtcgctcggc tggggcgtcg
     8221 agaccggcct ggacgagctg gtccgcgacc tcgcgatctt cgggatcggc ttcggactga
     8281 cggtgtcacc gcgggccacg gcggcggtcg aggcggccgg gtccggcgcc tacggcgtgg
     8341 ccagcgccat gctgcagatc acgcgcacgg tgggcatgag catcggcctg gcgctgttga
     8401 cgtccatcgg gcagaaccgg atcgacgagc tctcgcagct ggtcaacgat ccggcacggc
     8461 gcgacgagct ggtgcggacg ctcggtcgcc ccgagttcgt gggcgtggac ccgcaggcct
     8521 cgctggccct ggtggacctg ctcgagaact ggtcgcgcag ccaggcggcc ggcgtcctcc
     8581 agctggtgtt cgcgatcgcc ctcggcgtgg cggtggtgac cctcctcccc gcctggttcg
     8641 tcagcggcag gcggcgcgcc tgagtcggcg gggtcggcgc tcccggctca gaactcgggg
     8701 tcggcgacga cgacgagctc tccccagttg cggttgtaac cctgcgaggt ctgcagcgtg
     8761 aggcgctcgt ggtcggtcgg ctgggtccag aacactccct cgtcgcagtc gtcgtgcacc
     8821 tggagggcga gcggcggcgt gatcccgaag tcctcggggt tgttggtcgt ggcgtccggg
     8881 tcggggcagg cgacgttggg ccgcacctcg gtgaccacgt agcgcaggac ctgctcgttg
     8941 gacatcagga ccagcacctc cgcgccgacc tgcacgttcc acagcggctt gaagaggtcg
     9001 ttgaccgcgt gggcgaagac gtacgcgttg gtgttgtggc ccggctggtc gggggcctgc
     9061 aggttgccgc tctcgtccgt acggcagatg aggtaggcgc cgtcggacgg cgggaaggcg
     9121 cagtgcgacg catcggtcgg ggcgaagacg ttgacccgga tgccgaccga gggcacctcg
     9181 agccggacgg ccacgacgtc gtcgggcagc ggggtcggcg tcggcgtggc ggtcgcggcg
     9241 gcgctggccg acggttcccg ggtcggctcg agggtggcgc tcgacgtcgc ccgggcgctg
     9301 gctgagggtg tggccgtcac ctccgcgatc ggtggcagcg tcgggccggg cggaccgccg
     9361 gagtccaggg agcccgcgat aatgagggcg accccggcca ccccgagaat gatcgggagc
     9421 caccacccgg agagacgctt cacgtgaccg agtctagccg ccgaccgacg ccatcgccga
     9481 cggggtcccc gacgggccag ccgacggcct cgcctctggc cccgccttcg gcctcgctgt
     9541 ccgaggccct ggagctcgcg cgcgacgtcc tctccgcccg tccggccggc gtgctgaccg
     9601 atttcgacgg cacgctctca cccatcgtca ccgatccggc gctcgcgcgc ctggtggagg
     9661 gaggccacgg cgcgctggcg cggctggccg agcagctcga ggtcgtcgcc atcatcaccg
     9721 gccgcgcggc gctggacgcg cgccgcatga ccggcgtgcc cggcctgctg atcgccggca
     9781 accacgggac cgagtggctg gaacccaccg cggacgagcc ggtggcggcg ccggggatgg
     9841 aggacgtccg atcgcgcctc gaccgcgcgc tggcgcggct gcccgagctc gagggggtgc
     9901 cgatcgagca caagggcctc tccgccgcca tccactaccg caacgcgccg gatccggcgg
     9961 ccacgcgccg cgcgatcctc gacggcctcg gcgacgtcgg cggagagggc ctggagctgc
    10021 gccacggccg catgacggtc gagctgcggg ccatcggcgc cgccgacaag gggacggcga
    10081 cgcgagcgct cgtcgagcgc cacgccctgc gtgccgtggt ggtgctgggc gacgacgtca
    10141 ccgacctgga catgttccgg gccgtcatcg cactgcgcgc cgagggtcgg gtccgcggcc
    10201 tggtggtcgg cgtcggcggc ggggatcacg aggtgcccga ggaggtcagg ggcgcggccg
    10261 acgtgctgct gcgggatccg gcggaggcgg cggccttcct ggagggcctc acccgcgtct
    10321 gagcgccgag cctgtcaggc cgccaggcgt cgctcggccg ccgcccgtcg gtccgcggcc
    10381 ccgaaccacg cccagtacag cagcgtggcg atcgagtaga gcgagatcac ggtcaggaag
    10441 ttcacggcgt agccgccggt gaagccgagc gtggcctgga gcagcgcgta ccacccaccg
    10501 ccgacgaccc agccgatctg ccacagcagg ctcatcgacg ccgagagcgt cgcgcgctcg
    10561 gcgggcgaga cgtgctccat ggcgaaggcg ctgaagatcg ggttgcccgc gttcatcagc
    10621 gagttgcgga cgaccatggc cagcacgacc gtccacagga tcggtgagaa gcccagcacc
    10681 accaggaacg ggatgctgaa ggcctgcacg atcacgacgg acgtgatctg cccgaaccgc
    10741 cgcgccatgg cgggctgggc gagcatggcg gcgaccgttc ccaggctggt gaacgcgaag
    10801 agtgcgttga gggacgccag ttccagcccg aacttctgct gcacgaagac gttgaggaac
    10861 gggatcacct gcccggcacc gatggcgatg aggaagcccg gcagcaggag tctcacgaac
    10921 cggcgtcgat cccggatcac gatccccatg cgtgccggtg aggtggcgcg ttgtggcggg
    10981 aacgcggccg gctcgccgat cgccttcagc cgcgccgcgc tccgcatgcg cgagggccgg
    11041 tcgtccccca gcagcccgat ggtgaccagg ccgaccatca gcaggccgac catgatcacc
    11101 aggatgaccc ggtaggtcgt cgggccgtcc ggatcgctgc ccatggcgcc ggcgatcgcg
    11161 gtcgccacga tgccgcccag cgccgccgcg acgacgttcg tcacggtctg gatggctgcc
    11221 tgcagcgcga acaggtcgtt gcggtgctcg gcgtcgctgt gctccgccag gaacggcgcc
    11281 tggaccacca tcagcgactg ctggccgatg gcccacagcg ccgcgaagcc gatgatcagc
    11341 ggcagcgcct ccaccgacag caggccgatg agcgccagca cgcccacgat cacgcccccg
    11401 gccatcaccg cgcgacgacc gatgcggtcc gacagcgcgc tggccggcag ggcggtcacg
    11461 gcgccggcgg tcgacgagat ggtcgccacc aggccgacgg tggcggtcga gaagccgagc
    11521 gagcgcaggt agaggttgaa gtcgatccag tacaggctca gcgcggcgcc gagcaccagg
    11581 gtcgagacca ggaagacgcg agcgtcgcgc tggaagcggc cgatgcgcac caggtcggga
    11641 acgccccagc ggcgtcgcgg cgcgggaggc tgcaccatcg gtccgtcagc cggcgatgtc
    11701 ggcgaggtcc tcgagctgcc gccgcagcca ccaggtaatg tcctcgcgct ccacgccggc
    11761 ccgcagcaac cgcagccgcc gccgacgctc gtcgggttcc atggcgagcg ccgtcgcgat
    11821 cgcgtcggcc gtgccgacca cgtcggccgg ggccacggcc agcgcgccgt ccgccatctg
    11881 gtcgaaggcg ccggccgtct cggacaggac gagcaccgag tcgtgctgcg agacgacgac
    11941 ggcctccttc gccaccaggt tcatgccgtc gaccagcggg ttgacgagga ccacgtccgc
    12001 gatcgacagc ccggccagcg cctgggcgta gtcgttctcg tagaacagct ggaccggccg
    12061 ccactcggcg ttgccgaacc tggcgttgat gcgatcgacc gcgttctgca cctggcggcc
    12121 gtagtagccg tactcgcgca gcccggtccg cgacggcacc aggaaggcga agaagacgat
    12181 ccggttgcgc agccgcgggt tgcgctggag cagcgactcg aacgccagga agccgcgcag
    12241 gatgttcttc gacggctcga ggcggtcgac gcggaccagg gtggcctcgc gggtgcggcc
    12301 gcgcaggtcg tcggcgcgtc gccgcgccgc gcgcgagtgc gccacccgtc gggtcgcggc
    12361 cacgtcgatg ctgatcgggt acgagcgcac gtgggtggtg cgccgtccac ggcgcaccgt
    12421 cgacgactcg tagtcgacct tcgcgtcggg cacgaacgat tcgaccatgc gcaggaagtt
    12481 gtgggcgtag cgcgccgtct ggaagcccac cacgtcgttg gcgagcaggc cggtggcgat
    12541 cgactcgcgg atcgtcggcg cgatcgtcag ccagatgctg gacggaggcc acgggatgtg
    12601 ggtgaagtgg ctgaggacgg ccttggggcg cgcgcggcgt atcggctcgg cggccaggta
    12661 gaggtggtag tcgtggagca tgatgcgcgg ctcccggtcg ccccggacct gggccagcac
    12721 cgcgctggcg aacgcctcgt tgagcgccac gtagccacgc tcccaggcgc gcatcaacgc
    12781 cgcgtcgatg accggccgct ccggcaggtt ccacatctgg tgctgcagga accacaggaa
    12841 cgggttggcg atgacgttat aggcctgctc gtagacgttg cgctcgacgg tggcgaatcg
    12901 gagccgcacg tcctgcccat cgacggcggc cgctcgctcc agcagcttgg ggtccgccgc
    12961 ggcgcggcgg tcgccatcac ccatggccgc cgccacccag gtcaccggca cgtggcggcc
    13021 gacctggccc agggccgtga ccagtccgcc cgatccgcgg cgcggccgca ggccgccgtc
    13081 cgcggcggtc gtgaaggtca ccggaccccg gttggtcgcg atgatgaggc ggcggtcccc
    13141 caaccgggcc gacgccgccg cgcccagggc gctgtcggca tcgtgcggca tgggcgtcag
    13201 aagaggctcg ccgcggagac ctcgaggagg ccgaggatcg ctcccgggaa gatgcccagg
    13261 accaggacgc cggcgacgga cagcgccagc gc
//
LOCUS       DASTYP010000169         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_996986, whole genome shotgun sequence.
ACCESSION   DASTYP010000169 DASTYP010000000
VERSION     DASTYP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996986"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFV59_07760"
     CDS             <1..471
                     /locus_tag="VFV59_07760"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5325764.1"
                     /translation="GALSVALLEMGATRVTGVDLSAASVELARRRAVAAGFGELARYE
                     VGNAADVETEPQDWVILDRVICCFPDMDRLVRHAAPLAGSAFVLSVPESRGWRGLLNR
                     VAWDVEAAWDTLRGGCRGYVHDVRRIERRLGDAGLRRTDSLHLGLWYLGRYERS"
     gene            502..1113
                     /locus_tag="VFV59_07765"
     CDS             502..1113
                     /locus_tag="VFV59_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5325765.1"
                     /translation="MSRIANRTRREYRSELRAQQAEETRVRILDATVRLAARGLATTS
                     IPAVAREAGVSVPTVYRHFRTKEELFAALYPHVVYRSASVQLSMPGSIDEFGPMLRTQ
                     FAHLDAMGDVERVALASPAGEEARRAQMPNRIALTRKLVDGIVPDGSPVDRERLTRLL
                     VVLTGTAAFRTWHDHVGSSVDQAAEDVEWAVRALIAASTEDES"
     gene            1110..>2242
                     /locus_tag="VFV59_07770"
     CDS             1110..>2242
                     /locus_tag="VFV59_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEU5325766.1"
                     /translation="MSSDGVRRLADVRMADVAAVGGKAAGLGELLALNANVPDGVVLT
                     LDWAAAPPDARAEAVARALAELGDGPFAVRSSGISEDGSEHSFAGMFATFLEVPAGGV
                     AAAAERCLESAADARATAYASGATQPMAIIVQRMVAPAAAGVALTADPISGDRSTCVV
                     TAVRGLGERLVAGEALGDEWVVNGMKATPRRQPERSIDARQARTVADEARRIADARGA
                     PQDVEWVIDQAGDLWIVQARPMTALPPEVSWEPPAPGAYHRALRLGEWIGEPVTPLFE
                     SWLLSDLEVGLHAFIRRELGQRAPLPHHVIVNGWYFYSLNWIAPGNMLRSLPSILWHL
                     VRDPRSVGGVLPATVRHSFPIMERRWRRDVQPRYRAAVADAEAR"
BASE COUNT          298 a          769 c          839 g          336 t
ORIGIN      
        1 ggtgcgctga gcgtcgcgct gctcgagatg ggggcgacgc gcgtgacggg cgtggacctg
       61 tccgcggcga gcgtcgagct ggcgcgccgt cgagccgttg ctgcaggctt cggtgagctc
      121 gcacgctacg aggttgggaa cgcggccgac gtcgagacgg agccgcagga ctgggtgatc
      181 ctcgaccggg tcatctgctg cttcccggac atggatcgcc tggtccgtca cgctgcacca
      241 ctggctggct ccgccttcgt cctgagcgtc ccggaatccc gtgggtggcg gggcctgctg
      301 aaccgcgttg cgtgggacgt cgaagcggcc tgggacaccc tgcgcggcgg ctgccgcggg
      361 tacgtccacg acgttcggcg catcgagcgt cgtctcggcg acgccggctt gcgccgcacc
      421 gattcgctcc acctcggcct ctggtacctg gggcggtacg agcgcagctg acggggttga
      481 cacgactcct atattcacga aatgagtcgt atcgccaacc gcactcggcg ggagtaccgg
      541 agcgagctgc gggcacagca ggcggaggag acccgcgtcc gcatcctgga cgccacggtc
      601 cgactggcgg cgcgaggcct ggcgacgacc tcgatccccg cggtggcgcg cgaggccggc
      661 gtctccgtcc ccaccgtgta ccggcacttc cgcaccaaag aggagctgtt cgcggccctc
      721 tacccgcacg tggtctaccg atcggcgtcg gtgcagctct ccatgcccgg ctccatcgac
      781 gagttcgggc cgatgctgcg aacgcagttc gctcacctgg atgccatggg cgatgtcgag
      841 cgggtcgccc tggcgagccc tgccggcgag gaggcccgac gcgcgcagat gccgaaccgc
      901 atcgcgctga cccgcaagct ggtggacggg atcgtgcccg atggctcccc ggtcgaccgc
      961 gagcgcctga cccggctcct ggtggtcctg accggcaccg ccgccttccg aacgtggcac
     1021 gaccacgtcg ggtcgtccgt cgaccaggcg gccgaggacg tcgagtgggc ggttcgcgcc
     1081 ttgatcgccg cgagcacgga ggacgagtca tgagctccga tggtgttcgt cgcttggccg
     1141 atgtccgcat ggccgacgtg gctgcggtcg gcggcaaggc cgccggcctc ggtgagctgc
     1201 tggcgctcaa cgccaacgtt cccgacgggg tggtgctgac gctcgactgg gcggcggcgc
     1261 cgccggatgc aagggccgag gcagtcgctc gggcgcttgc ggagctgggc gacgggccct
     1321 ttgccgttcg atccagcggc atcagcgagg acgggtcaga gcactccttc gcgggcatgt
     1381 tcgcgacctt cctcgaggtc ccggcgggcg gggtcgcggc cgccgccgag cgctgcctgg
     1441 aaagcgcggc ggacgctcga gccaccgcct acgcctccgg tgcgacacaa cccatggcga
     1501 tcatcgtcca gcgcatggtc gcgccggccg ccgcgggggt cgcgctcacg gcggatccga
     1561 tctccgggga ccgatcgacc tgcgtcgtca ccgccgtacg gggactgggc gagcgtctgg
     1621 tggccggcga ggcgctgggc gacgagtggg tcgtcaacgg gatgaaggca acgccgagac
     1681 gccagcccga gcggtcgatc gacgcgcggc aggcgcggac ggtggcggac gaggcgcggc
     1741 ggatcgccga cgcgcggggc gcgccgcagg acgtggagtg ggtcatcgac caggcgggcg
     1801 acctgtggat cgtgcaggcg agaccgatga cggcgcttcc gccggaggtc tcctgggagc
     1861 caccggcgcc cggcgcctac caccgggcgc tcaggttggg cgagtggatc ggtgagccgg
     1921 tgaccccgct gttcgagtcg tggctgctct ccgaccttga ggtcgggctg cacgccttca
     1981 tccgtcgcga gctcggtcag cgagcgccgc tgccgcacca cgtcatcgtc aacggctggt
     2041 acttctactc gctcaactgg atcgcgccgg ggaacatgct ccgcagcctg ccgagcattc
     2101 tctggcacct ggtgcgggac ccgcggtcgg tcggcggggt gctcccggcc accgtgcggc
     2161 acagcttccc gatcatggag cggcgctggc gacgtgacgt ccagccgagg taccgcgccg
     2221 ccgtggccga tgcagaggcg cg
//
LOCUS       DASTYP010000170         5409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_670220, whole genome shotgun sequence.
ACCESSION   DASTYP010000170 DASTYP010000000
VERSION     DASTYP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670220"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VFV59_07775"
     CDS             complement(<1..1307)
                     /locus_tag="VFV59_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU5325767.1"
                     /translation="MVATDPTLLDAAQVARDLETDARNGLTSEEAARRLAVHGPNRLA
                     AAPPVPAWRKLLGQFADPLIYLLLVAIVVSVVAWWLEGAEGLPIEAIVIATIVLANGV
                     LGFVQERQAERAVAALQRMAAPTAHVVRDGTPRHVAASELVPGDVLLLAEGDTVSADG
                     RLETAASLMVAEAALTGESEPVLKDPVPLPAPAGIGDRFDMVFAGTAVTRGRGRAIVT
                     ATGMGTEMGRIATLLEQTQPERTPLQREIDVLGRWLGIAVIVIAVVVVGAVLLTSDIT
                     GLGDLVDVLLLGVSLAVAAVPEGLPAILTVVLALGVQRMAVQRAIVKKLSSVETLGSA
                     SVIGSDKTGTLTRNEMTIVRIATRSGTVEVTGSGYRPEGQVLADGAPIEAGPLHGEVR
                     AVLSGGSLANDAVLLNQAGSWTVQGDPTEAAFLVAERKLGTTE"
     gene            1415..1822
                     /locus_tag="VFV59_07780"
     CDS             1415..1822
                     /locus_tag="VFV59_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018219757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5325768.1"
                     /translation="MIEQETRLDIQAAAWTNDAFRREVLTGKHEQVVVMTIPPGGEIG
                     DEVHPDNDQVLVFVDGNGEAIIDGERSPVAPNDLVFVHAGTRHNFVNTGDGPMRLITV
                     YAPPHHAPGTVHQTKEEADAAEAGEAAGGTDEE"
     gene            complement(1982..2425)
                     /locus_tag="VFV59_07785"
     CDS             complement(1982..2425)
                     /locus_tag="VFV59_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEU5325769.1"
                     /translation="MSQAALPAQLAEIVEDFASAPPRDRLEMLLEYSQSLPPLPERFA
                     GHPERMEPVPECQSPLFVATEVGPDHRVSIYADAPPEAPTTRGFAGILHAGLDGLPAE
                     EVLAVPGDVTARLALAEVVSPLRLRGAAALLGRIQNQVRTQAGTR"
     gene            complement(2422..3309)
                     /locus_tag="VFV59_07790"
     CDS             complement(2422..3309)
                     /locus_tag="VFV59_07790"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5325770.1"
                     /translation="MALPNDPDPKLAQYAHPEKLVTTAWLAEHLDDPGLVVVESDEDI
                     LLYETGHIPGAVKVDWHTELNDQVTRDYLDGERFAELMRAKGIGRDHTVVFYGDKSNW
                     WAAYALWVFSLFGHPDVRLLDGGRATWEAEGRPMTTDAPDRRPTDYPVVQRNDGAIRA
                     YREDVLAHLGKPLIDVRSPDEYTGKLLHMANYPQEGALRGGHIPGAKSVPWARAANED
                     GTFKSREELDALYGGEAGLTPDDEVVVYCRIGERSSHTWFALTHLLGYEHVRNYDGSW
                     TEWGNSVRVPIERDVEGSA"
     gene            3504..4031
                     /locus_tag="VFV59_07795"
     CDS             3504..4031
                     /locus_tag="VFV59_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5325771.1"
                     /translation="MTGDHGAFESLVGPEIERLYGLAGLLLNDRARAEDAVQEALLKA
                     WRDLPKLREVAKFHAWLRRLVVNASHDEGRRLGRRRREVELKPSHDRADSDGLDPLLD
                     RDELSQAFRRLKQEERTVVALRYFMDLSTADAAAALGIREVTYRSKLHRAIRTLGAAL
                     AADARSTARPEGRWT"
     gene            4028..4870
                     /locus_tag="VFV59_07800"
     CDS             4028..4870
                     /locus_tag="VFV59_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325772.1"
                     /translation="MSTDRTGDDFERRLSQSLHAAAPRPSGEVAHRLLSRTAAEHQRR
                     GFSGLRFAPMLAGAAVVAGAVVLGLFFANLLPRNESVGPAPTTSSASALPSSTPAAPS
                     TSATETPAPSVSPSAPAADDVLTCTNPEIGYAASYPADWWANERVEPDFQGGDAVTAC
                     TVFAEEPVAIRPNSELPRGVAISIGVSDVVYPPSSGATVVSQEDAIVAGRPAVVREDE
                     QTSSEDPFSYPGQRRYSYFVALPDGRYLVAVTSSQQDQADAVYQEHRRVLDALMDSLD
                     LTGA"
     gene            complement(4883..>5409)
                     /locus_tag="VFV59_07805"
     CDS             complement(4883..>5409)
                     /locus_tag="VFV59_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5325773.1"
                     /translation="GYAERLRSEHRIPVFVHEADAARARGEIKPKTSMGKVRIRPLIR
                     FLWYAGRRGGLRTTYLREVQTFRGGETLDLPGSPRVIPMPGHSPGSVAFHVPSVDAVF
                     VGDALTTRDVLTGAGGPRPAPFTDDPDGAAASLEALEALDAHWVLPGHGPPWEGGSAE
                     AVRRYRAAVAAAAS"
BASE COUNT          789 a         1930 c         1940 g          750 t
ORIGIN      
        1 tgctccgtcg tccccagctt gcgctccgcg acgaggaacg cggcctcggt cggatcgccc
       61 tgcaccgtcc agctgccggc ctggttcagc aggaccgcgt cgttggccag cgagccgccg
      121 gacaggacgg cgcgcacctc gccatggagt gggcccgcct cgatcggcgc cccgtcggcc
      181 agcacctggc cctcgggacg gtagccactg ccggtgacct ccacggtccc cgatcgcgtc
      241 gcgatgcgca cgatggtcat ctcgttgcga gtgagcgtcc cggtcttgtc ggagccgatg
      301 acggaggcgg agcccagggt ctcgaccgac gacagcttct tcacgatcgc gcgctgcacc
      361 gccatgcgct gcacgccgag cgcgagcacc acggtcagga tggccggaag gccctccggg
      421 accgcggcga ccgccagcga cacgccgagc aggaggacgt cgacgaggtc accgaggccg
      481 gtgatgtccg acgtcaggag cacggcgccg acgaccacga ccgcgatcac gatgaccgcg
      541 atccccagcc accgcccgag gacgtcgatc tcccgctgga ggggggtgcg ctcgggttgc
      601 gtctgctcga gcagcgtggc gatgcggccc atctcggtgc ccatgcccgt ggccgtcacg
      661 atggcgcggc cgcggccgcg cgtcacggcc gtgccggcga agaccatgtc gaagcggtcg
      721 ccgatgccgg ccggcgcggg caggggcacg ggatccttga ggaccggctc gctctcgccg
      781 gtcagcgccg cctcggcgac catcagcgag gcggccgtct cgagccgccc gtcggcgctc
      841 acggtgtccc cctcggcaag caagaggacg tcacccggaa cgagctcgct ggccgcgacg
      901 tggcgcggtg ttccgtcgcg cacgacgtgg gccgtcgggg ccgccatccg ctgcagcgcg
      961 gcgacggctc gctccgcctg ccgctcctgg acgaagccga ggacgccgtt ggccagcacg
     1021 atcgtcgcga tcacgatcgc ctcgatcgga agcccttccg cgccctcgag ccaccacgcc
     1081 acgacgctga cgacgatggc cacgaggagc agatagatga gcggatcggc gaactggccg
     1141 agcagcttgc gccaggcggg caccggcggc gcggccgcca gccggttcgg cccgtggacc
     1201 gcgagacggc gggcggcctc ctcactcgtc agtccgttcc gcgcatcggt ctcgaggtca
     1261 cgagcgacct gggcagcatc gaggagggtc gggtcggtgg cgaccatgcc acgagtatgg
     1321 gcgtcaccgc ccggccgcga tcgtgctgtt cggaccttgc ccggggtgcc gctggatcgg
     1381 accatcggcc cgagccattc caggaggagc agcgatgatc gaacaggaga cccgcctcga
     1441 catccaggcc gccgcgtgga ccaacgacgc cttccggcgc gaggtcctca ccgggaagca
     1501 cgagcaggtc gtcgtcatga cgatcccgcc gggtggcgag atcggcgacg aggtccaccc
     1561 agacaacgac caggtcctcg tcttcgtgga tggaaacggg gaggcgatca tcgacggcga
     1621 gcggtccccg gtggcgccca acgacctggt cttcgtgcac gccggcacgc gccacaactt
     1681 cgtcaacacc ggcgacggac cgatgcgtct catcaccgtc tacgccccgc cgcaccatgc
     1741 gcccggcacc gtgcaccaga cgaaggaaga ggccgatgcg gccgaggccg gggaggcggc
     1801 cggcggaacc gacgaggagt agcttgcgac gcctcaccgg cgccgatgca tggggttgat
     1861 cacagcgcac gcgaccggct acggacgcgc tgcttgattc gccccaggca tcgggcggcg
     1921 cgggaccaga cgttcgcgcg cgctgtgatc agccggagct atcgcggccg cagtgcgcgg
     1981 ctcagcgagt tcccgcctgc gttcggacct ggttctggat ccgtcccagc aacgccgctg
     2041 cgccccgcag gcgcagcggg ctcaccacct ccgccagggc aagacgggcg gtgacgtcgc
     2101 cggggacggc gaggacctcc tcggccggca gcccgtcgag tccggcgtgc aggatcccgg
     2161 cgaagccgcg ggtggtcggt gcctcggggg gcgcatcggc gtagatggac acgcggtggt
     2221 ctggtccgac ctccgtggcg acgaagagcg gcgactggca ctccggcacc ggctccatcc
     2281 gctcagggtg tccggcgaag cgctcgggaa gcggcggcag cgactgcgag tactcgagca
     2341 gcatctcgag ccggtcgcgc ggcggtgccg acgcgaagtc ctcgacgatc tccgccagct
     2401 gcgccggaag cgcggcctgg ctcacgctga cccctccacg tcccgctcga tcgggacgcg
     2461 gacgctgttg ccccactcgg tccaggagcc gtcgtagttc cgcacgtgct cgtagcccag
     2521 caggtgggtc agcgcgaacc aggtgtggct cgaccgctca ccgatgcggc agtacacgac
     2581 cacctcgtcg tcgggcgtca ggccggcctc gccgccgtag agcgcgtcga gctcctcgcg
     2641 cgacttgaag gtcccgtcct cgttcgcggc ccgtgcccag ggcaccgact tcgcccccgg
     2701 gatgtggccg ccgcgcagag cgccctcctg cgggtagttc gccatgtgca gcagcttgcc
     2761 ggtgtactcg tccggcgagc ggacgtcgat cagcggcttg cccaggtggg cgagcacgtc
     2821 ctcgcggtag gcgcggatgg cgccatcgtt gcgctggacg accgggtagt cggtgggtcg
     2881 gcggtcgggc gcgtcggtcg tcatcggtcg accctcggcc tcccacgtgg cgcggccgcc
     2941 gtcgagcagt cggacatccg ggtgaccgaa gaggctgaag acccacagcg cataggcggc
     3001 ccaccagttc gacttgtcgc cgtagaagac gacggtgtgg tctcggccga tgcccttcgc
     3061 gcgcatgagc tcggcgaatc gctcgccgtc gaggtagtcg cgcgtgacct ggtcgttgag
     3121 ctcggtgtgc cagtcgacct tcaccgcgcc ggggatgtgg ccggtctcgt agaggaggat
     3181 gtcctcgtcc gattcgacga ccaccaaacc ggggtcgtcc aggtgctccg ccagccaggc
     3241 ggtcgtcacc agcttctcgg gatgggcgta ctgcgcgagc ttgggatcgg gatcgttcgg
     3301 gagagccatt cgccgatgct atgccaaaca gagcggtcgt gctcatgcac catctggtcg
     3361 agctgcgcaa cggtgtagtg cgcgccgcgc gtaaggcggg gcagctgaat tcgcaacggc
     3421 cggccgaccc ggagcgccag tacgggtgac gccgccaagg aatggggtaa cggatcttgc
     3481 acgaggagct cgtccgacga gcgatgaccg gtgatcacgg cgcgttcgag tcgctggtcg
     3541 gcccggagat cgaacgcctg tacggcttgg ccggcctgct gctcaacgat cgggctcggg
     3601 cggaggacgc ggtccaggaa gcgctgctca aggcatggcg tgacctgccg aagctccgcg
     3661 aggtcgcgaa gttccatgcc tggctgcgcc gtctggtcgt caacgcctcg cacgacgagg
     3721 gccggcgcct gggtcgtcgg cgccgtgagg tggagctgaa gccctcccac gaccgagccg
     3781 atagcgacgg cctcgatccg ctcctcgacc gggacgaact gtctcaggcc ttccggcggc
     3841 tgaagcagga ggagcggacc gtcgtggcgc tccgctactt catggatctc tcgacggccg
     3901 atgcggcggc cgcgctcggc atccgcgagg tcacctaccg cagcaagctg caccgcgcca
     3961 tcaggacgct cggggccgcg ctggccgccg acgcgcgctc cacggcgcgg ccggagggac
     4021 gatggacatg agcacggacc gcaccggcga cgacttcgag cgtcgtctct cgcagtcgct
     4081 ccacgcggcg gcgccgcggc catcgggcga ggtcgcccac cgcctgctga gccgcaccgc
     4141 ggcggagcat cagcggcgcg ggttcagcgg gctgcgcttc gcgcctatgc tggcgggtgc
     4201 ggcggtcgtg gcgggggccg tcgtattggg gctcttcttc gcgaacctgc tgccgcgcaa
     4261 tgagagcgtg gggcccgcgc cgacgacctc ctcggcgagc gcgctcccct ccagcacgcc
     4321 ggcggcgccg agcacttccg cgaccgagac gcccgcgccg tccgtctccc ccagcgcacc
     4381 ggccgcggac gacgtcctga cgtgcacgaa cccggagatc ggctacgccg cctcctaccc
     4441 ggccgactgg tgggcgaacg agcgggtgga accggacttc cagggcggcg acgccgtgac
     4501 ggcgtgcacc gtcttcgccg aggagccggt cgccatccgg cccaactcgg agctgcctcg
     4561 cggcgtcgcg atcagcatcg gcgtctccga tgtcgtctac ccgccgtcga gcggcgcgac
     4621 ggtcgtctcg caggaggacg ccatcgtggc gggccggccg gccgtggtgc gggaggatga
     4681 gcagacctcg tccgaggatc cgttcagcta tccggggcag cggcgctaca gctacttcgt
     4741 ggcgctgccc gacgggcgct acctggtggc ggtgaccagc tcacagcagg accaggccga
     4801 tgcggtctac caggagcacc ggcgcgtgct cgacgcgctg atggacagcc tggacctgac
     4861 cggtgcctga ggacgccgcg gttcaggagg ccgcggccgc gaccgcggcg cggtatcgcc
     4921 gcaccgcctc cgctgatcca ccttcccacg gcggaccgtg acccggcagc acccagtgag
     4981 cgtccaacgc ctcgagcgcc tccagcgatg ccgcggcgcc atcgggatca tcggtgaacg
     5041 gtgccggcct cgggccgccc gcgccggtca gcacgtcgcg cgtcgtcagc gcgtcgccca
     5101 cgaagacggc atccaccgac ggaacgtgga acgcgacact ccccggggag tggcccggca
     5161 tcgggatcac gcgcggtgag ccgggcaggt cgagcgtctc tccaccgcgg aacgtctgga
     5221 cctcgcgcag gtacgtcgtg cgcagccctc ctcggcgccc cgcgtaccac aggaagcgga
     5281 tcagcggacg gatgcggacc ttgcccatcg acgtcttggg cttgatctcg ccgcgcgccc
     5341 gcgcggcatc ggcctcgtgg acgaagaccg ggatgcggtg ctcgctccgg agtcgctcgg
     5401 catagccga
//
LOCUS       DASTYP010000171         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_888054, whole genome shotgun sequence.
ACCESSION   DASTYP010000171 DASTYP010000000
VERSION     DASTYP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888054"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1194
                     /locus_tag="VFV59_07810"
     CDS             199..1194
                     /locus_tag="VFV59_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU5325774.1"
                     /translation="MPRVAIVFTGGTISMRQDGAAGNRPTLRGDDLLASVPGLDAIAD
                     IEPIDWGLVPASHLTLAQVLEIGRILDEQLRRPEIVGAVVVQGTDVIEETAFAWDLLP
                     LTAKPVVVVGAMRSASQEAYEGPENLRNAVRAAADPRLAGQGVVVAMAGELHGADDVR
                     KTHTHAFATFQSPNAGRLGLVGDTGVTLLRQRQPMRLAAIPDAAALPVPIIGVALDLD
                     PGLLRASIGEAPAGLVVAAAGGGNTPPVYLELARPLLDRGVPVVLTTRCPSGRPLPGY
                     GFPGGSTQWWEAGALFSGTLDALKARLVLALGLGAGADRAALVRVFEAFGGGSRG"
     gene            complement(1214..2599)
                     /gene="pyk"
                     /locus_tag="VFV59_07815"
     CDS             complement(1214..2599)
                     /gene="pyk"
                     /locus_tag="VFV59_07815"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5325775.1"
                     /translation="MTRPSRATKVVCTIGPASVDRVAELVDAGMDVARINFSHGTPER
                     HAETARRVRDAAAAGDRSVALMADLPGPKIRLGDVPDGRVELRAGERFVLGEGGATVS
                     YAGLGTDVEPGDRILLADGAAELRVVETGASVVTEVVRGGAVRSRAGVNVPAERLSTP
                     SLTERDRADVPRAQQLRVDYVAQSFVRRAADVEELRGLLGPDGPRIVAKIETRPAVDD
                     YDAICDAADAVMIARGDLGVELPYEQVPILQKQLIRHALDRGVPTIVATQMLESMISS
                     PRPTRAEASDVANAIFDGADAIMLSGETAIGQFPVLAAQAAVRVAQLCETDGRAYLPS
                     GAPTSARTDADALAVAAVALAHADADVAAIACYTRTGRTARLLAALRPHVPILAFSPE
                     ARVVDQLALVRGVRSRGCVPPEATEGRLSLMAWLVGESSLAENSAVVLVASTAEPGTG
                     PNLLEVHRIRR"
     gene            2895..>3346
                     /locus_tag="VFV59_07820"
     CDS             2895..>3346
                     /locus_tag="VFV59_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325776.1"
                     /translation="MPASEQFQPAAAPAAPSGGSRWLALLARFWWIPVAILVAVGGSL
                     FAASRDDAGQITDGGDLGVADLRPGDCFDLKDASEELIDDVDAKPCSETHEFEMFFVG
                     SLSGDAYPAEETMTGYVIDNCIPAFDAYIGMDYQSSVYDIFHFVPSEDG"
BASE COUNT          425 a         1219 c         1239 g          463 t
ORIGIN      
        1 gcgcaggtcg agaccgcgca gggcatgggt ctcgccgaag gacttgtgga ggccggcggc
       61 ctcgatcatg gtgcggctca aggccggcct cgtgctcggt gggggccgga atgataccgc
      121 ggctcggccg cccggtcctg cacgcgactc ggccatgcac gcccgcgcgc cgggacggac
      181 gcgcgtagca tcgcggccat gccacgcgtc gcgatcgtct tcaccggcgg cacgatctcc
      241 atgcgccagg acggcgcggc cggcaaccgc ccgacgctcc gcggcgacga cctcctcgca
      301 tccgtccccg ggctcgacgc gatcgccgac atcgagccga tcgactgggg cctcgtcccg
      361 gcctcccacc tgacgctggc ccaggtgctc gagatcggcc gcatcctcga cgagcagctg
      421 cgccgtccgg agatcgttgg cgccgtcgtc gtgcagggca ccgacgtgat cgaggagacg
      481 gccttcgcgt gggacctgct gccgctgacg gccaagccgg tcgtcgtggt cggagccatg
      541 cgctcggcgt cgcaggaggc gtacgagggt cccgagaacc tgcgcaacgc cgtgcgcgcc
      601 gcggctgatc cgcgcctggc gggccagggc gtggtcgtcg cgatggccgg cgagctgcac
      661 ggcgccgatg acgtgcggaa gacgcacacg cacgccttcg ccaccttcca gagcccgaac
      721 gccggccgcc tcggcttggt gggcgacacc ggcgtcaccc ttctccggca gcgccagccg
      781 atgcgcctcg ccgccatccc cgatgctgcc gccctgccgg tgccgatcat cggcgtggcg
      841 ctggacctcg atcccggcct cctgcgcgcg tccatcggcg aggcgccggc cggcctggtc
      901 gtggccgcgg ccggcggcgg caatacgccg ccggtctacc tcgagcttgc gcgtccgctg
      961 ctggaccgcg gcgtgccggt ggtgctgacc acgcggtgtc cgtcgggccg ccccctgccg
     1021 gggtacggct tccctggtgg cagcacgcag tggtgggagg ccggcgcgct gttcagcggc
     1081 acgctcgacg cgctcaaggc caggctggtg ctcgcgctgg ggctgggtgc cggcgcggac
     1141 cgggcggccc tcgtccgagt cttcgaggcg ttcggcggcg ggtcgcgcgg ctgagacgcg
     1201 tcgcatgcgg cggtcagcgc cgaatgcggt gcacctcgag caggttcggt ccggttcccg
     1261 gctcggccgt cgaggccacc aggaccaccg cgctgttctc ggccagcgag ctctcgccga
     1321 ccagccaggc catcagcgac aggcggccct cggtcgcctc gggcggcacg cacccccgcg
     1381 aacggacccc gcgcaccagc gccagctggt cgaccacgcg tgcctccggc gagaacgcca
     1441 ggatcgggac gtgcggccgg agtgccgcca gcagccgcgc ggtgcggccc gtgcgcgtgt
     1501 agcacgcgat ggccgcgacg tccgcatcgg cgtgggccag ggccacggcg gcgacggcca
     1561 gcgcgtccgc atcggtccgg gcggaggtcg gggcaccgga gggcaggtag gcgcgtccat
     1621 cggtctcgca cagctgcgcg acgcggaccg ccgcctgggc ggcgaggacc gggaactgcc
     1681 cgatggccgt ctcgccggac agcatgatgg cgtcggcccc gtcgaagatc gcgttggcca
     1741 cgtcgctggc ctcggcccgc gtcggccgcg gggacgagat catcgactcg agcatctggg
     1801 tcgcgacgat cgtcggcacg ccgcggtcga gcgcgtgacg gatgagctgc ttctggagga
     1861 tggggacctg ctcgtagggc agctccacgc ccaggtcgcc gcgggcgatc atgaccgcat
     1921 cggcggcgtc gcagatcgcg tcgtaatcgt cgacggcggg gcgcgtctcg atcttcgcca
     1981 cgatcctcgg cccatccgga cccaggagcc cgcgcagctc ctcgacgtcg gcggcgcgac
     2041 gcacgaacga ctgggcgacg tagtcgacgc ggagctgttg ggcgcgcggc acgtcggcgc
     2101 ggtcgcgctc ggtcagtgag ggtgtggaga gtcgctcggc cggcacgttc acgccggcgc
     2161 gcgatcggac cgcgccgccc cgcaccacct ccgtgacgac ggacgcgccg gtctcgacga
     2221 cgcgcagctc ggccgcccca tcggccagga ggatgcgatc gcccggctcg acgtccgtgc
     2281 ccaacccggc gtagctgacc gtggcgccgc cctcacccag cacgaatcgc tcacctgccc
     2341 gcagctcgac gcggccatcc gggacgtcgc ccaggcggat cttcggaccg ggcaggtcgg
     2401 ccatgagcgc cacgctccgg tcgccggccg ccgccgcgtc gcgcacgcgg cgcgcggtct
     2461 cggcatggcg ttccggcgtc ccgtggctga agttgatccg cgccacgtcc atgcccgcgt
     2521 cgaccagctc ggcgacgcga tcgacggacg cggggccgat ggtgcatacc accttggtgg
     2581 cgcgcgatgg gcgggtcacc ccgcaagcct gcgggatgcg gcgcgctcgc tgcaagccgt
     2641 gtcgccatcg ggcaccatgg cgggcggtac cgtcgtcatg ggccgatcga tgggtggagg
     2701 tgctgccccc gctgcccgac cgtaccgtgt tgccgcccgt ccattgaccc cgaccgagcg
     2761 gctgacgacg ctggggtcgg acagacggag ggaatcgggc tttggctgat cggtgggtgt
     2821 gcaagcgctg cttcgcggcg aacgacgaga gcgcgggcca atgtgcggcg tgtgggctcg
     2881 tccggggctc ggaggtgccg gcatcggagc agttccagcc ggccgccgcc ccggcggcgc
     2941 cgagtggcgg ctcgcgctgg ctcgcgctcc tggcgcgctt ctggtggatc ccggtcgcca
     3001 tcctcgtcgc ggtcggtggc tcgctgttcg cggccagccg tgacgacgcc gggcagatca
     3061 ccgatggcgg cgacctcggc gtcgcggacc tgcgtcccgg cgactgcttc gatctgaagg
     3121 acgccagcga ggagctcatc gacgacgtcg acgccaagcc gtgcagcgag acgcacgagt
     3181 tcgagatgtt cttcgtcggc tcgctgagcg gcgacgcgta cccggccgaa gagaccatga
     3241 ccggctacgt gatcgacaac tgcatcccgg ccttcgacgc gtacatcggg atggactacc
     3301 agagctcggt gtacgacatc ttccacttcg tccccagcga ggacgg
//
LOCUS       DASTYP010000172         5930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_377947, whole genome shotgun sequence.
ACCESSION   DASTYP010000172 DASTYP010000000
VERSION     DASTYP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377947"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFV59_07825"
     CDS             complement(<1..680)
                     /locus_tag="VFV59_07825"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5325777.1"
                     /translation="MKRIPALVAMFVAALLVACGAQVTPTASGEVDPSRSPRPSSTAT
                     PASPSASAPPSDAPTAEWQAANVPPTDDGTATVQVVPGGEGLIAIGFDGGFGSILWTS
                     ADGGRTWTDVTPAGFESIGLATVIEHDGRLIGVGRGNTIDVETEEAAVYLSDDGLTWS
                     KVETAEQLIGQMIDVVATDDGLFAVGGVPGADSAGVWRSADGETWERIGSDLEHAFLW
                     SIAEGGPGL"
     gene            970..2292
                     /locus_tag="VFV59_07830"
     CDS             970..2292
                     /locus_tag="VFV59_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5325778.1"
                     /translation="MTNPTPLSVAVVGAGISGLTAAFELHKAGHRVRLFESEATLGGH
                     ATTVETPDGQPVDIGFIVYNERTYPRFIAMLDELGVETQPSDMSMGVSCAACGVEWST
                     RGLSGVFANRRSLARPSHYAMLNDLFRFYRDARRTLDEADAGRPTNLTLDEYLADRGL
                     GRAFANHFLVPLTAAVWSTAPDTIGSFPVDYLLRFLDHHGLIGYGNAIQWRTVTGGSH
                     RYVEAIAERLGPTAISTGDPVRSIRRDSVGVTVTRTRGAPERFDAVVMATHGDVTRRL
                     LHDADAQERHALEGLEYSDNRVVLHTDRSVLPRRTPAWASWNIDMGDCTRPGDALTMT
                     YHMNRLQSLPGETQYSVSLNAPQGRIDPSTVLLERDWAHPLYTFRTLEAQREIASLQG
                     RNRTWYAGAHLGYGFHEDGCRSGYEAAAAIDRWSQTAVETEPMEERAA"
     gene            2289..3053
                     /locus_tag="VFV59_07835"
     CDS             2289..3053
                     /locus_tag="VFV59_07835"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEU5325779.1"
                     /translation="MRSHLLEGKTRHRRSRPFVYTLEHDVWYVALDLAELDEVDRRHR
                     LLSRNRWNLLSLRDRDFFLPAASDLDASVRQHLRAEGFEAKDWRITFVATPRVAGYQF
                     NPASFYLCRDEADALRVVIVEVHNTHGERRLYTLRPAEKGTAHVAGMAKDFYVSPFIG
                     MDAAYTVRVQDRDDQLRIVIDETEEGQPLLQASVVLKRRRLNDLALARMLVRYPFVTM
                     KTIVMIHWHALKLWRRGAKFHSHSDAAAPPVREVAA"
     gene            3050..4297
                     /locus_tag="VFV59_07840"
     CDS             3050..4297
                     /locus_tag="VFV59_07840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU5325780.1"
                     /translation="MTQRAVTSAGPLFGNPLLADAAQRILLGALARIRIGRLTVVLPD
                     GSRRSFGERESDVRGEIHVHDRAALVRLLIDGETGAGEAYMDGQWTSPDLGALLRLAA
                     LNRAALSLTGGWWRVPTQWLRVVAHRLNRNTVDRARRNIASHYDLSNDLYRLFLDESM
                     TYSSAVYSAETATLEEAQREKYRRMAALAGIERGMHVLEIGSGWGGFALYAAGELGAR
                     VTTITISERQLALARERVALAGLSDLVTVELRDYRQVRGQYDAIVSIEMLEAVGHEYF
                     GAYFAAIDRLLRPGGRAAIQSITYPDAAYEQQRRGANWIQKYIFPGGLLPSLAVIERS
                     TAGTSLLLTGVEDIAPSYARTLAEWRARFMGSLPSVRALGFDERFIRMWEYYLALCEA
                     GFAAGIFQDLQIGFEKRGGTFRD"
     gene            complement(4465..5232)
                     /locus_tag="VFV59_07845"
     CDS             complement(4465..5232)
                     /locus_tag="VFV59_07845"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5325781.1"
                     /translation="MFRIGEFARLGGISAKQLRAWDELGLFRPAWVDRWSGYRYYSPA
                     QLPELRRILALRQLGMGLDEILALVRGGEVRAALERRREELERERRVVEERLAALDIS
                     VGAEAGPDVVVRPVAVEPVAVMRVPARVSDAFYALESYVRDLGRRAHRPPGALPSEGE
                     IFVPVTGPIPDTDAIAYRRLPAGRVAAVIHRGSYHGVAAARGTLLRWVEAAGLAATGP
                     MRTLYLQFGAEPELRLPPGWVVERDDDLVTELQLPVT"
     gene            5283..>5930
                     /locus_tag="VFV59_07850"
     CDS             5283..>5930
                     /locus_tag="VFV59_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU5325782.1"
                     /translation="MQLHETRPIRIGAQLHPQHGDWRDLRAAALRAEELGYDILYTWD
                     HFSPLYGEPDGKHFECWSLLAAWAEATERIELGPLVACNSYRNPDLHADIARTVDHIS
                     GGRVIMGLGAGWFERDYRRYGYEFGTRARRIRALGEATPRIVERLRAGLNPPPVRRMP
                     ILIAGVGRTLTLPIVARHADAWHAFFPDTVEEVVPAVEALLAHCAAIGRDPYEIEW"
BASE COUNT          893 a         2146 c         2067 g          824 t
ORIGIN      
        1 aggcccgggc cgccctcggc gatcgaccac aggaaggcgt gctccaggtc ggagccgatg
       61 cgctcccacg tctccccatc ggccgagcgc cagacgccgg ccgaatcggc tccggggacg
      121 cctcctaccg cgaacaggcc atcatcggtc gcgaccacgt cgatcatctg gccgatcagc
      181 tgctcggcgg tctcgacctt tgaccaggtc aggccatcgt cgctgaggta gacggcggcc
      241 tcctcggtct cgacgtcgat ggtgtttcct cggccgacgc cgatcagccg gccatcatgc
      301 tcgatgacgg tcgccaggcc gatggactcg aagccggccg gcgtcacatc ggtccacgtg
      361 cgcccaccgt cggcggaggt ccacaggatc gagccgaacc cgccatcgaa gccgatggcg
      421 atcagccctt ccccacccgg gacgacctgg acggtcgccg tgccgtcgtc ggtgggaggg
      481 acgttcgccg cctgccactc cgcggttggt gcgtccgatg gtggtgcgga tgcggacggt
      541 gacgcgggcg tcgccgtcga cgagggccgc ggcgaacgcg acgggtccac ctcgccggag
      601 gcggtcggcg tgacctgtgc accgcacgcg acgagcagcg cggccacgaa catggccacg
      661 agggcaggga ttcgcttcat gcgagcagcg tggcggtgac cggtcggtcg ggcatcggtc
      721 gagcgacgta ggaaggtgcg gcgggtggac gtagcgccgt ccggcgcacg aagttgtccg
      781 ccagcggaac caacggacac gcaggacccc gaggtcgccg ctgcgtcgcc gcccgttgtc
      841 atcgggcgag ccggacagcg acggacgggc tccagtgacc gcgaacttcg ccgttagttc
      901 cggtggcgag caactcggcg agcgagcgcg ccactgatcc gatggcgccg caccgcacgt
      961 accggcggcg tgaccaaccc cactcccctg agcgtcgccg tcgtcggcgc gggcatcagc
     1021 ggcctcaccg cggcgttcga gctgcacaag gcggggcatc gggtgcgcct cttcgagagc
     1081 gaagcgacgc tcggcggcca tgccaccacc gtcgagacgc cggacggaca gccggtcgac
     1141 atcggcttca tcgtctacaa cgagcggacc tacccgcggt tcatcgccat gctcgacgag
     1201 ctcggcgtgg agacgcagcc gtccgacatg agcatgggcg tcagctgcgc ggcgtgcggg
     1261 gtcgaatggt ccacgcgcgg gctcagcggg gtgttcgcca accgacgcag cctggcgcga
     1321 ccgagccact acgccatgct caacgacctc ttccgcttct atcgggacgc ccgacggacg
     1381 ctggacgagg ccgatgccgg ccgcccgacg aacctcaccc tggacgagta cctggccgac
     1441 cgcggcctgg gtcgcgcctt cgcgaaccac ttcctcgtgc cgctcaccgc ggccgtgtgg
     1501 tcgaccgcgc cggacaccat cggcagcttc ccggtcgact acctgctgcg cttcctcgac
     1561 caccacgggc tgatcggtta cggcaacgcg atccagtggc ggacggtgac cggcgggtcg
     1621 caccgctatg tcgaggccat cgcggagcgc ctcggaccga ccgccatcag caccggcgac
     1681 ccggtgcgca gcatccggcg cgactcggtc ggtgtgacgg tcacccgcac ccgcggcgcc
     1741 cccgagcgat tcgacgccgt cgtcatggcg acgcacggcg acgtcacccg ccgcctcctc
     1801 cacgacgcgg acgcacagga gcgccacgcg ctcgagggcc tcgagtactc ggacaaccgc
     1861 gtggtgctac acacggaccg gagcgtcctg ccgcgcagga cgccggcgtg ggcgagctgg
     1921 aacatcgaca tgggcgactg cacgcggccc ggcgacgccc tcaccatgac ctaccacatg
     1981 aaccgcctcc agtcgctgcc gggtgagacc cagtacagcg tcagcctgaa cgcgccccag
     2041 gggaggatcg atccgtcgac cgtcctgctg gagcgcgact gggcgcaccc gctctacacg
     2101 ttccgaaccc tcgaggcaca gcgcgagatc gcgagcctcc agggccgcaa ccgcacctgg
     2161 tacgcgggcg cgcacctggg ttacggcttc cacgaggatg gctgccgctc gggatacgaa
     2221 gcggccgccg ccatcgaccg ctggtcgcag acggccgtcg agaccgagcc gatggaggag
     2281 cgcgccgcgt gagatcgcac ctcctcgaag gcaagacccg gcatcggcga agccggccgt
     2341 tcgtgtacac cctggagcac gacgtctggt acgtcgcgct ggacctggcg gagctggacg
     2401 aggtcgaccg gcggcatcgg ctgctcagcc gcaaccgatg gaacctgctc agcctgcgcg
     2461 accgcgactt cttcctgccg gccgcatcgg acctggacgc cagcgtgcgt cagcaccttc
     2521 gcgccgaagg cttcgaggcg aaggactggc gcatcacgtt cgtcgcgacc ccccgcgtgg
     2581 cgggctacca gttcaacccg gcctcgttct acctctgccg ggacgaggcc gatgcgctcc
     2641 gcgtcgtcat cgtcgaggtc cacaacacgc atggcgagcg gcggctgtac acgctgcgcc
     2701 ccgccgagaa gggcacggcg cacgtcgccg gcatggccaa ggacttctac gtcagcccgt
     2761 tcatcggcat ggatgccgcg tacacggtgc gggtgcagga ccgcgacgac cagctgcgga
     2821 tcgtgatcga cgagaccgag gagggtcagc cgctgctgca ggcatcggtc gtgctcaagc
     2881 gtcggcggct gaacgatctg gcgcttgccc gtatgctcgt gcggtacccg ttcgtgacca
     2941 tgaagaccat cgtgatgatc cactggcacg cgctcaagct ctggcgccga ggggcgaagt
     3001 tccactcgca ctcggatgcc gctgccccgc ccgtccgcga ggtggccgca tgacccagcg
     3061 cgcggtgacc tccgccggtc cgctcttcgg gaaccccctc ctcgccgatg ccgcccagcg
     3121 aatcctgctg ggtgccctgg cgcgcatccg catcggccgg ttgacggtgg tgctgccgga
     3181 cggctcgcgc cgctccttcg gggagcggga gtccgacgtc cggggcgaga tccacgtcca
     3241 cgatcgagcg gcgctggtcc gcctcttgat cgatggcgag accggggccg gagaggccta
     3301 catggacggc cagtggacct ccccggacct cggcgcgctg ctgcggctgg cggcgctcaa
     3361 ccgggcggcg ctgtcgctca ccggcggctg gtggcgcgtc ccgacgcagt ggctgcgggt
     3421 cgtggcgcat cggctcaacc gcaacacggt cgaccgggca cgccggaaca tcgcctcgca
     3481 ctacgacctg tcgaacgacc tgtaccggct cttcctcgac gagtcgatga cgtactcctc
     3541 cgcggtctac agcgcggaga cggcgacgct cgaggaggcg cagcgcgaga agtaccgccg
     3601 gatggccgcg ctggccggga tcgagcgcgg gatgcacgta ctcgaaatcg gttccggctg
     3661 ggggggcttc gcgctgtacg cggccggcga gctcggggcg cgggtcacga ccatcacgat
     3721 ctcggagcgg cagctggccc tggcgcgcga gcgggtggcc ttggcggggc tgtcagacct
     3781 ggtgaccgtc gagctgcgcg actaccgcca ggtccggggg cagtacgacg ccatcgtctc
     3841 gatcgagatg ctcgaggcgg tcggccatga gtacttcggc gcctacttcg cggccatcga
     3901 ccgattgctg cgcccgggcg gccgcgcggc gatccagtcc atcacctacc ccgatgccgc
     3961 gtacgagcag cagcgccgcg gcgccaactg gatccagaag tacatcttcc cgggtgggtt
     4021 gctgccgtcg ttggccgtga tcgagcggtc gacggcaggg acgtcgctgc tgctcacggg
     4081 ggtggaggac atcgcgccgt cctacgcgcg gacgctggcc gaatggcggg ctcggttcat
     4141 gggctcgctg ccgtccgtcc gtgcgctggg cttcgacgag cggttcatcc ggatgtggga
     4201 gtactacctc gcgctgtgcg aggccgggtt cgcggccggc atcttccagg acctgcagat
     4261 cggcttcgag aagcgcggcg ggacgttccg ggactgaccg cggccccggg ttgctccacg
     4321 acggaacgaa cggcgaagtt gaccgcgttt cggccgccac cgtccgctcc agtcgcgcct
     4381 cgcggcaact gcgggccaag gaaccggccg ttccggtggc tccacgtccg ttggttccgc
     4441 tggacgacaa ccgcacctct cccgctacgt cacgggcagc tggagctccg tgacgaggtc
     4501 gtcgtcacgc tccaccaccc agccgggtgg cagccgcagc tcgggctccg cgccgaactg
     4561 gaggtagagc gtccgcatcg gtcccgtcgc ggccaggccg gcggcctcca cccagcgcag
     4621 cagcgtcccc cgcgccgccg caaccccgtg gtacgacccg cggtggatga cagcggccac
     4681 gcggccggcg ggcagacggc ggtacgcgat cgcatcggtg tcggggattg gcccggtgac
     4741 gggcacgaag atctcgccct ctgagggaag cgcccccgga ggtcgatgcg cccgccgacc
     4801 gaggtcgcgc acgtacgact cgagcgcgta gaaggcgtcg gagacgcgag ccgggacgcg
     4861 catgaccgcc accggttcga ccgccaccgg ccggacgacc acatccggcc cagcctcagc
     4921 gccgaccgag atgtcgaggg cggccaggcg ctcctccacg acccgccgct cccgctccag
     4981 ctcctcgcgc cggcgctcga gggcggcacg cacctcgcca ccgcggacga gggcaaggat
     5041 ctcgtccagc cccatgccga gctggcggag ggccaggatg cgccgcagct cgggcagctg
     5101 ggcgggcgag tagtagcggt agccgctcca ccggtccacc caggcggggc ggaacagccc
     5161 cagctcatcc caggcacgca gctgcttggc gctgatgccg ccgagccgcg cgaactcacc
     5221 gatgcggaac atatgagccg ataccttgac tttcccctca ggggaaggtc aagcatcggc
     5281 gcatgcagct tcacgagacc agaccgatcc ggatcggggc ccagctccac ccgcagcacg
     5341 gcgactggcg ggacctgcgg gccgccgcgc tccgcgccga ggagctcggc tacgacatcc
     5401 tctacacctg ggaccacttc agcccgctct acggcgagcc ggacgggaag cacttcgagt
     5461 gctggtcgct gctcgccgcg tgggccgagg cgaccgagcg gatcgagctc gggccgctgg
     5521 tggcctgcaa ctcgtaccgc aacccggatc tccacgccga catcgcgcgg accgtcgacc
     5581 acatcagcgg cggccgggtg atcatgggcc tcggcgccgg ctggttcgag cgcgactacc
     5641 gccgctacgg gtacgagttc gggactcggg cgcggcgcat ccgcgcgctg ggcgaggcga
     5701 cgccccgcat cgtggagcgg ctgagggccg ggctcaatcc gccgccggtg cgccgcatgc
     5761 cgatcctgat cgccggcgtc ggtcggaccc tgaccctgcc gatcgtcgcc cgccacgccg
     5821 atgcctggca cgcgttcttc cccgacaccg tcgaggaggt cgtgccggcg gtcgaggcgc
     5881 tgctcgcgca ctgcgccgcg atcggtcgcg acccgtacga gatcgagtgg
//
LOCUS       DASTYP010000173         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_232953, whole genome shotgun sequence.
ACCESSION   DASTYP010000173 DASTYP010000000
VERSION     DASTYP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232953"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..727)
                     /locus_tag="VFV59_07855"
     CDS             complement(374..727)
                     /locus_tag="VFV59_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEU5325783.1"
                     /translation="MTDTDPTCLFCRIVAGDLPSRLAYTDENVVAFHDVNPQAPTHVL
                     IVPRRHVPDIDALTPDDGELLAALFGAVQRVAADAGFEGGYRVVSNVGPDAGQSVPHL
                     HLHVLGGRQMGWPPG"
     gene            complement(740..1432)
                     /locus_tag="VFV59_07860"
     CDS             complement(740..1432)
                     /locus_tag="VFV59_07860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEU5325784.1"
                     /translation="MTLHRFFVDPAAMVGDRFPLPATIERQVRTVLRLRDGERIVLLT
                     GDGDELTCQLEGDDCVVESRQRAGGEPAHRLTVVQALLKGDGLEEVVQRGTELGVVRF
                     DLTVTDRCVARELSARKLERLRAIARESAEQSERGIVPHVAAPVPVSDLLHPPAVLLF
                     EREVERRLRDLDQPPTSVVIGPEGGFSEREVALARERGVEVVGLGRRILRSQTVAPAV
                     AAVVLSRSGDFA"
     gene            complement(1429..2346)
                     /gene="prmA"
                     /locus_tag="VFV59_07865"
     CDS             complement(1429..2346)
                     /gene="prmA"
                     /locus_tag="VFV59_07865"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU5325785.1"
                     /translation="MRWLELSVEADVEAVEAVSEILGRVSEGTAVHPSALLRDPNDEL
                     AVRADAGAPYVVTAHIADDADAARRIDATERALWHLQAFGLRPLGGLRVRAVDDASWA
                     DAWKEHYVPQRVGRVVIVPSWIEEPLAAGEVAITLDPGMAFGTGLHPTTRGCLTLLQE
                     ISPMPAQVLDVGSGSGILALAALRLGAERAVCLDTDPLAVEASVENARRNGLEARLQA
                     RQGSLPPTADRGYPLVLANLVAAVLIELAPRLAAHTATHGVLLASGIIAPRADEVVEA
                     LKRAGFALDQRLEDGEWVSLRLRRTGAAA"
     gene            complement(2358..3485)
                     /gene="dnaJ"
                     /locus_tag="VFV59_07870"
     CDS             complement(2358..3485)
                     /gene="dnaJ"
                     /locus_tag="VFV59_07870"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5325786.1"
                     /translation="MAVKRDYYDVLGVPREADEAEIKRAFRRLAQQHHPDVDKSDGAE
                     ERFKELNEAYRVLSDRQRRTAYDMFGHAGVEGSAAGGFEGFGGGFGPFGDIFDAFFGG
                     APAGTRRRQRVVPGSDLRYDLVIEFAEAVFGVTKEITFPTLVTCTNCGGSGGEPGSEP
                     ETCPDCNGTGERRRVAQTILGQMVNIVTCTRCRGEGKIIANPCAVCGGDGRVREERTL
                     QVTVPAGIDDGQRIALEGQGEAGPRGGPPGDLYVVIKVRPHPELIRRGTELYHVLPVT
                     FPQAALGATLTVPTVEGSEEVEVPAGVQSGHEIRLRGKGVPRLRGAGRGDLHVVINVV
                     VPTKLSKRERELLRELNEVGAPATLPKDGPSIIDRLRDLFS"
     gene            3571..>4349
                     /locus_tag="VFV59_07875"
     CDS             3571..>4349
                     /locus_tag="VFV59_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU5325787.1"
                     /translation="MTQRTLSADHARRFLVSRHLLAPPRSLPADPASVLDVVERLGSL
                     QFDPLEVPGARNHDLVLHARVAGYQRAWCDAWLYGPDRRLIELYNKSLNILPMHELPH
                     YRIAWQRSSSHYEDTILKEQAAVADAILRRLETDAPLSTAAFGEHGHAVDWWWAPTRA
                     ARAVMEALFVTGRIGIARRDGNRRFYDLIERLVPADLLERRESEEDAMAHRLLSRFRA
                     VGLTTPIGTQSEVMYSAGSTAERARRTERLVDEGRLIPVSV"
BASE COUNT          617 a         1640 c         1495 g          597 t
ORIGIN      
        1 gcgcgctctc gaacgtctcg ttctcgccca cgcggacttc tgccaaacgt ctcgcctcct
       61 tcccgctctg gtctcgtgcc gatctgcatg gcgggacgac cccgccgggc acgcaagccc
      121 gcgaagggta ccagtgcgac cacgaccggt caatttggtg aacgcggcgc tccggcgctc
      181 cggcgctggg aacggtcgca attcacacca ggtgggcagg aagccgtcgg gcgccccgtt
      241 gaaggatgct cattcacacc acgcgtgaat cgaaaccggt caaggcgcct ccacgggctc
      301 tcagcgtcga cgacgcccgc tcgctgcgca cctcgtgtga attcggcccc tcggcttgcg
      361 caaccgagcc gcctcaaccc ggcggccacc ccatctggcg cccgcccagg acatggagat
      421 ggaggtgcgg caccgactgg ccggcgtcgg ggccgacgtt gctgaccacg cggtacccgc
      481 cctcgaatcc ggcgtcagcg gccacgcgct gaacggctcc gaagagcgcg gccagcagct
      541 cgccgtcgtc gggcgtgagg gcgtcgatgt cggggacgtg gcggcggggc acgatcagca
      601 cgtgggtcgg cgcctgcgga ttgacgtcgt ggaaggccac cacgttctcg tcggtgtacg
      661 cgaggcgaga cggtagatcg cccgcgacga tgcggcagaa gagacaggtc ggatcggtat
      721 cggtcatgac cgcagcaggc taggcgaagt cgccgctgcg cgacaagacg acggcggcga
      781 ccgccggcgc caccgtctgg gatcgcagga tccgacgccc gaggccgacc acctcgaccc
      841 cacgctcgcg cgccagcgcc acctcgcgct cgctgaagcc gccctccgga ccgatcacga
      901 cggacgtcgg gggctggtcc aggtcgcgca ggcgccgctc cacctcgcgc tcgaacagca
      961 gcacggccgg tgggtgcaac aggtccgaca ccggcaccgg cgcggccacg tgcggaacga
     1021 tcccgcgctc ggactgctcg gccgactcgc gggcgatcgc ccgcagccgc tcgagcttgc
     1081 gtgccgacag ctcgcgggcc acgcagcggt cggtgacggt caggtcgaag cggaccacgc
     1141 cgagctccgt cccgcgctgc accacctcct ccagcccgtc gcccttcagc agcgcttgca
     1201 ccaccgtgag ccgatgcgcc ggctcgccgc cggcgcgctg ccgtgactcg accacgcagt
     1261 cgtcaccctc cagctggcag gtgagctcgt cgccgtcgcc ggtgagcagc acgatgcgct
     1321 cgccgtcccg caaccgcagg accgtacgca cctggcgctc gatcgtcgcc gggagcggga
     1381 agcggtcccc gaccatggcg gccggatcca cgaagaaccg atgcagcgtc atgccgccgc
     1441 cccggtgcgt cgcagccgca ggctgaccca ctccccgtcc tccagccgct ggtcgagcgc
     1501 gaagcccgct ctcttcagcg cctcgacgac ctcgtcagcg cgcggcgcga tgatcccgct
     1561 ggccagcagg acgccgtgcg tcgcggtgtg ggccgccagc cgtggcgcga gctcgatcag
     1621 cacggcggcg accaggttgg ccagcaccag cgggtagccg cggtccgcgg tgggtgggag
     1681 cgagccctgg cgtgcctgca gtcgagcttc gaggccgttg cgacgggcgt tctcgaccga
     1741 tgcctccacg gccagaggat cggtgtcgag gcagacggcc cgctccgctc cgagccgcag
     1801 cgccgccagg gccaggatgc cggacccgct gccgacgtcg agcacctgcg cgggcatcgg
     1861 cgagatctcc tggagcaggg tcaggcagcc gcgggtggtc ggatgcagcc cggtaccgaa
     1921 cgccatcccc ggatcgagcg tgatcgccac ctcgcccgcg gccaggggct cctcgatcca
     1981 ggacgggacg atgacgacgc ggccgacgcg ctgcgggacg tagtgctcct tccaggcatc
     2041 ggcccacgac gcgtcgtcga cggcgcggac ccggaggccg cccagcggac gaagtccgaa
     2101 ggcctgcagg tgccacagcg cgcgctcggt ggcgtcgatc cgtcgcgcgg cgtcggcatc
     2161 gtcggcgatg tgcgccgtga cgacgtacgg cgccccggcg tccgcgcgca ctgccagctc
     2221 gtcgttcggg tctcgcagca gggcgctggg gtgcaccgcc gtgccctccg acacgcgccc
     2281 caggatctcg gacaccgcct ccaccgcctc gacgtcggct tcgacgctca gctcgagcca
     2341 gcgcatcggc tcgaccgtca gctgaagagg tcgcgcaggc gatcgatgat cgacggaccg
     2401 tccttcggca gcgtggccgg ggcgccgacc tcgttgagct cacgcaggag ctcgcgctcg
     2461 cgcttcgaca gcttcgtcgg cacgacgacg ttgatgacca cgtgcaggtc gccccgaccg
     2521 gcgccacgca gccgcggcac gcccttgccg cgcagccgga tctcgtgccc ggactgcacg
     2581 ccggccggca cttcgacctc ctccgagccc tcgacggtcg ggaccgtgag cgtggcgccc
     2641 agggccgcct gcgggaacgt caccggcagc acgtggtaca gctcggtgcc gcgccggatg
     2701 agctccggat gcggtcgcac cttgatcacg acgtacaggt cgccgggcgg gccaccgcgg
     2761 ggaccggcct cgccctggcc ctccagcgcg atgcgctggc cgtcgtcgat gccggccggc
     2821 acggtgacct gcagcgtccg ctcctcccgg acccggccgt cgccgccgca cacggcgcac
     2881 gggttggcga tgatcttccc ctcgccccgg cagcgcgtgc aggtcacgat gttgaccatc
     2941 tggcccagga tcgtctgcgc cacgcggcgc cgctcgccgg tgccgttgca gtcgggacac
     3001 gtctccggct cgctgcccgg ctcgccacca ctgccgccgc agttcgtgca cgtcaccagc
     3061 gtcgggaagg tgatctcctt cgtgacgccg aacaccgcct cggcgaactc gatgaccagg
     3121 tcgtagcgca ggtccgagcc gggaacgacc cgctgccgcc gccgcgtgcc ggccggcgcg
     3181 ccgccgaaga aggcgtcgaa gatgtcgccg aacggtccga agccgccgcc gaagccctcg
     3241 aaaccgccgg ccgcggaccc ctccactccg gcgtggccga acatgtcgta agcggtgcgc
     3301 cgctggcggt ccgacaggac gcgataggcc tcgttcagct ccttgaagcg ctcctcggca
     3361 ccgtcgctct tgtcgacgtc gggatggtgc tgctgggcaa gccgccggaa ggcccgcttg
     3421 atctcggcct cgtcggcctc tcgggggacg ccgagcacgt cgtagtagtc gcgtttcacc
     3481 gccatggcgc gcggatgata ccaactggca ctctcgtggt gcgagtgcca gtcccggtcg
     3541 cgcgtcgacg gccgctacga tgcgcccggc atgacccagc gcacgctctc ggccgatcac
     3601 gctcggcgct tcctggtctc tcggcacctg ctcgctccgc cccgctcgct gccggcggat
     3661 cccgcgtccg tgctcgacgt cgtcgagcgc ctcggcagcc tgcagttcga cccgctcgag
     3721 gttcccggcg cccggaacca tgacctcgtc ctgcatgcgc gggtcgcggg ctaccagcgc
     3781 gcctggtgcg acgcgtggct gtacggcccg gatcggcgcc tgatcgagct ctacaacaaa
     3841 agcctcaaca ttctgccgat gcacgagctg ccgcactatc gcatcgcctg gcagcgctcg
     3901 agcagccact acgaggacac catcctcaag gagcaggccg cggtcgccga tgccatcctg
     3961 cgccgcctcg agaccgatgc cccgctctcg acggccgcct tcggcgagca cggtcacgcc
     4021 gtcgactggt ggtgggcgcc gacgcgggcg gcgcgcgccg tgatggaggc gctgttcgtc
     4081 accggacgga tcggcatcgc ccggcgcgac ggcaaccgcc gcttctacga cctcatcgag
     4141 cgcctcgtcc ccgcggacct cctggagcga cgcgagtcgg aggaggacgc catggcgcat
     4201 cggctcctga gccggttccg cgcggtgggc ctcaccacgc cgatcgggac acagagtgag
     4261 gtgatgtaca gcgccggctc gacggccgaa cgggcgcggc gcaccgagcg gctggtcgac
     4321 gaggggcggc tcatcccggt gagcgtcga
//
LOCUS       DASTYP010000174         4618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1035287, whole genome shotgun sequence.
ACCESSION   DASTYP010000174 DASTYP010000000
VERSION     DASTYP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035287"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..469)
                     /locus_tag="VFV59_07880"
     CDS             complement(92..469)
                     /locus_tag="VFV59_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5325788.1"
                     /translation="MDRIIQLLINAAALYVAVVIVPNLEFTGEWWKLLVVAFIFGLVN
                     TFLRPILRVLTLPITLMTLGIFLLILNALLLLLVSAISGELNLGFTVGDFLAALLGSI
                     VISLVGLVLSMVIGTGRMAGRLL"
     gene            complement(479..1819)
                     /locus_tag="VFV59_07885"
     CDS             complement(479..1819)
                     /locus_tag="VFV59_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5325789.1"
                     /translation="MARLPERPPFALRVRLLTPTDDGGWIDEVDGLIEVDAAGRIASA
                     GAWDQARAPASVLDVRPLVLLPGLVDLHAHLPQLPNAGLGSGLDLLTWLDRYIFPLER
                     AFDRPTAERQAPAAFAAFAAAGTTTVVAYSALWADSTDAAFAAAEGHGIRAVIGKVMM
                     DRITYDAEIDPSTILETSLRQSDELASRWHGRDDGRLQYAFTPRFAVSCTAEMLRESA
                     TLARSHGAYWQTHLSEDAGEMDEVRRLFPGARDYLDVYDDARALGPKTIFAHAIHLSA
                     PEVDRLVESRCVVAHCPASNLFLSSGMMPLAAYRRAGVRLGLGSDVAAGPETSIFTVM
                     RAGAVTQRALELSGRADREGALHPLDWLRLASLDGARALDLADRIGSLESGKEADAIL
                     VDPRLTTPLPGDVAPSGADELLSRMIFRPHPNMVRGAWVRGRLLAGPPGLDGIG"
     gene            1879..2085
                     /locus_tag="VFV59_07890"
     CDS             1879..2085
                     /locus_tag="VFV59_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU5325790.1"
                     /translation="MASGSVKKVMADRGFGFITAEDGKDYFFHRDGVSATVDFDRLVG
                     GERVEFDIEPSARGPRATNVRATE"
     gene            2099..3244
                     /locus_tag="VFV59_07895"
     CDS             2099..3244
                     /locus_tag="VFV59_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5325791.1"
                     /translation="MRAVPDAYRGTRRTVGIVVLVAAILAGAAFLAWWVAARQDGPLR
                     TETVQSGLEHPWDLAFTDSGRMLVTERAGRVRVYASGERNAPLLTVSTIPDVRAELES
                     GLMGIAVAGDTVFVCATRGPAEDEATWHVDLLAATLAADGSLSTFEPMPIGPTIGGPR
                     HQGCAVQIGPDKMLWFSIGDANLPAGQTVAMDPTVLNGKVVRLNLDGSVPPDAAFPSG
                     VVSVGHRNPQGLAFRDDLVVEVEHGTDVNDEINVIEPGANYGYPCFTGAADPGPIPEG
                     CGPPSDYRPPAWASGDPTLATSGAVFLTGEGWGDWAGDLVVTTLKEEDLRRFVVDGSG
                     RVTLAETLLDRRFGRLRAAVIGPDHALYLSTSNGDDRIIRVTRSDEG"
     gene            3257..3943
                     /locus_tag="VFV59_07900"
     CDS             3257..3943
                     /locus_tag="VFV59_07900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5325792.1"
                     /translation="MTEETNDARTLLDEQIRYYRARASEYDATSMPPEGPMAASLRRV
                     QAAVAALGIRGRVLELAAGTGQWTELLVQTASELTAVDASPEMLRLNADKVRDSSVRY
                     VVSDVFELEPRAEWDVVFFSAWLSHVPSSHFAAFWTLVGGFLAPGGRAVFVDEAAPGL
                     GGETWLDERGGVVHRELSDGSRYRAVKILWAPETLVERLRELGWDAEVEADGVFLWGS
                     ATPRIDGGVL"
     gene            3940..4596
                     /locus_tag="VFV59_07905"
     CDS             3940..4596
                     /locus_tag="VFV59_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015938517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5325793.1"
                     /translation="MTGASPIRVTPILVADLHVEGDRMPVYVHLIDHPDARVLIDTGL
                     TQLHPAVVDMDPRLHPLREQGFDVASVDVVVNTHLHFDHCGGNHLFAGTPIHVQRQEL
                     EDARTQEDYTIPEWVDPPGVRYAPVDGEHELLPGVRLVPAPGHTRGTQVVVIEGGRRP
                     VVIAGDAAVWFGELDDPQTEGQRLIRSLDPELVWLSHAHEPWRPHQRRGRLEPEHMGG
                     "
BASE COUNT          693 a         1579 c         1709 g          637 t
ORIGIN      
        1 cgagggcgat cagcacggcc agcccgagcc cgaagagcag gcccaccggc cacggagcac
       61 gggccatggc gggtggcggc ggagaagccg tctagagcag ccggccggcc atgcggcccg
      121 tgccgatgac catcgagagc accaggccca ccaggctgat gacgatcgag ccgagcagcg
      181 ccgccaggaa atcgccgacg gtgaacccga ggttgagctc acccgagatg gcgctcacca
      241 gcagcagcag cagcgcgttg aggatcagca ggaagatgcc gagggtcatc agggtgatcg
      301 gcagggtcag gacccgcagg atcggtcgca ggaaggtgtt gaccaggccg aagatgaagg
      361 ccacgaccag cagcttccac cactcgccgg tgaactccag gttcggcacg atgaccaccg
      421 cgacgtacag cgccgcggcg ttgatcagca gctggatgat ccgatccatg ggcgtagcct
      481 agccgatgcc gtccaggccc ggcgggccgg cgagcaaccg accgcgcacc cacgcgccgc
      541 gcaccatgtt cgggtgggga cggaagatca tccggctcag cagctcgtcc gcgccggacg
      601 gcgcgacgtc accgggcagc ggcgtggtga ggcgcgggtc gaccaggatg gcgtcagcct
      661 ccttgccgga ttccagggag ccgatcctgt ccgccaggtc cagcgcgcgg gcgccgtcga
      721 ggctcgccag ccgcagccaa tcgagcgggt gcagcgcgcc ctcgcggtcc gcgcggccgc
      781 tcagctcgag ggcgcgctgc gtcaccgcgc cggcccgcat cacggtgaag atggacgtct
      841 cgggcccggc ggcgacgtcg ctcccgagcc ccagccggac gccggcgcgc cggtacgcgg
      901 ccaggggcat catgccgctc gacaggaaca ggttcgacgc cgggcagtgc gcgacgacgc
      961 accggctctc gaccaggcgg tcgacctcgg gggccgagag gtggatggcg tgcgcgaaga
     1021 tcgtcttcgg cccgagtgcc cgcgcgtcgt cgtagacgtc gaggtagtcg cgcgcacccg
     1081 ggaacaggcg ccggacctcg tccatctcgc cggcgtcctc ggagaggtgc gtctgccagt
     1141 aggcgccgtg cgagcgagcc agcgtcgcgg actcgcggag catctcggcg gtgcagctca
     1201 ccgcgaagcg cggcgtgaag gcgtactgga ggcggccatc gtcacggccg tgccagcggg
     1261 acgcgagctc atccgactgg cggaggctgg tctccaggat ggtcgaagga tcgatctcgg
     1321 cgtcgtaggt gatgcggtcc atcatcacct tgccgatcac cgcgcggatg ccgtggccct
     1381 cggccgccgc gaaggccgca tcggtcgagt ccgcccacag cgccgagtag gcgaccacgg
     1441 tggtggttcc cgcggccgcg aaggcggcga aggccgcagg ggcctgccgc tcggcggtcg
     1501 gtcggtcgaa ggcgcgctcc agcgggaaga tgtagcggtc gagccaggtg aggaggtcca
     1561 gcccgctgcc gaggcccgcg ttcggcagct gcggcagatg ggcgtggagg tcgaccagtc
     1621 ccggcagcag caccagcggc cggacgtcca ggaccgacgc cggcgcccgg gcctgatccc
     1681 aggcgcccgc agaagcaatg cggccggcgg cgtcgacctc gatgaggccg tcgacctcgt
     1741 cgatccagcc gccgtcatcg gtgggcgtga ggaggcgcac gcggagcgcg aagggcggtc
     1801 gctcgggaag gcgggccatg ggccggatgc tacacgccgg aacggagtac gcttgcagtt
     1861 cgttgtagga ggccaaccat ggccagtggc agtgtcaaga aggtcatggc cgaccgcgga
     1921 ttcgggttca tcaccgccga ggacggcaag gattacttct tccatcgcga cggcgtgagc
     1981 gcgaccgtcg acttcgaccg gctggtcgga ggcgagcgcg tcgagttcga catcgagccc
     2041 agtgcccgag gtccgcgcgc cacgaacgtc cgcgccacgg agtaagggtt ccgggtcggt
     2101 gcgagccgtc ccggatgcgt accgcggaac gcggcgcacg gtcgggatcg tcgtcctggt
     2161 ggcggcgatc ctcgccggcg ccgcgttcct cgcgtggtgg gtcgcggcgc ggcaggatgg
     2221 acccctgcgc accgagacgg tccagtccgg gctcgagcac ccgtgggacc tcgcgttcac
     2281 cgacagcggg cggatgctcg tcaccgagcg cgccggccga gtgcgcgtct atgcctccgg
     2341 cgagcggaac gccccactcc tgacggtctc gaccatcccc gacgtgcgcg ccgagctcga
     2401 gtccggcctc atggggatcg cggtcgcggg tgacaccgtc ttcgtctgcg ccacccgggg
     2461 gcctgccgag gatgaagcca cctggcacgt ggacctgctg gcggcgacct tggcggccga
     2521 cggctcgctc tcgacgttcg agccgatgcc catcggtccc accatcggcg ggccgcgcca
     2581 ccaggggtgc gccgtccaga tcggtcccga caagatgctg tggttctcca tcggcgacgc
     2641 caacctgccg gcgggccaga cggtggccat ggatcccacc gtcctcaacg gcaaggtggt
     2701 ccggttgaac ctcgacggct ccgtgccgcc ggacgcggcg ttcccctccg gcgtcgtctc
     2761 cgtgggccac cgcaacccgc agggcctggc cttccgcgac gacctcgtgg tcgaggtcga
     2821 gcacgggacc gatgtgaacg acgagatcaa cgtcatcgag ccgggcgcca actacggcta
     2881 cccgtgcttc acgggtgctg ccgaccccgg gccgatcccc gaggggtgcg gcccacccag
     2941 cgactaccgg ccgcccgcgt gggcctccgg cgaccccacg ctggcgacct cgggcgcggt
     3001 gttcctgacc ggcgaaggtt ggggcgactg ggcgggggat ctggtggtca cgacgctcaa
     3061 ggaggaggac ctgcggcgat tcgtcgtcga cgggtcgggt cgtgtgacgc tcgccgagac
     3121 gctgctcgat cggcgcttcg gacggttgcg ggcggccgtc atcggccctg accatgcgct
     3181 ctacctgtca acctcgaacg gcgacgaccg catcatcaga gtcacccgtt cggacgaggg
     3241 atgaggacgc tgcaccgtga ctgaggagac gaacgacgcg cgcacgctgc tcgacgagca
     3301 gatccgctac taccgggcgc gcgcatcgga atacgacgcg acctccatgc cgccggaggg
     3361 gccgatggcg gcgtcgctcc ggcgggtgca ggctgccgtc gcggcgctgg gcattcgtgg
     3421 ccgggtgctg gagctggccg caggcacggg gcagtggacg gagctgctgg tccagaccgc
     3481 ctccgagctg acggccgtcg acgcctcgcc cgagatgctc cggctgaacg ccgacaaggt
     3541 ccgcgattcg agcgttcggt acgtcgtgtc ggacgtcttc gagctcgagc cgcgggccga
     3601 gtgggacgtc gtgttcttca gcgcctggct gtcgcacgtg ccgagcagcc acttcgcggc
     3661 gttctggacg ctggttggcg gcttcctggc gccgggcggc cgcgccgtct tcgtggacga
     3721 ggcggcaccc ggtctcggtg gcgagacctg gctggacgag cgcggtggcg tcgtgcaccg
     3781 agagctgagc gacgggtcgc gctaccgggc ggtcaagatc ctgtgggcgc ccgagacgct
     3841 cgttgagcgc ctgcgcgagc tcggatggga cgctgaggtc gaagcggacg gcgtcttctt
     3901 gtggggctcg gcgacgccgc ggatcgacgg cggcgtctta tgacgggggc ctcccccatc
     3961 cgcgtgacgc cgatcctcgt cgcggacctg cacgtcgagg gcgatcggat gcccgtctac
     4021 gtgcacctca tcgaccaccc cgatgcgcgc gtgctgatcg acaccgggct gacccagctg
     4081 cacccggccg tcgtcgacat ggatccacgg cttcacccgc tccgggagca gggcttcgac
     4141 gtggcgagcg tcgacgtcgt cgtcaacacc cacctgcact tcgaccactg cggtggcaac
     4201 cacctcttcg ccggtacgcc gatccacgtg cagcgccaag agctcgagga cgcacgaacc
     4261 caggaggact acaccattcc ggagtgggtg gacccgcctg gcgtccgcta cgcgccggtc
     4321 gacggcgagc acgagctgct ccccggggtg cggctggtgc cggcacccgg gcacacgcgt
     4381 gggacgcagg tcgtcgtgat cgagggcggc cggcggccgg tcgtgatcgc cggtgacgcc
     4441 gccgtctggt tcggcgagct ggacgatccg cagaccgagg gccagcggct gatccggtca
     4501 ctggatcctg agctggtctg gctgtcgcac gcgcacgagc cgtggcggcc ccatcagcgg
     4561 cgcggtcgac tggaacctga gcacatggga gggtgacgag catcggctga gtggtcgg
//
LOCUS       DASTYP010000175        16601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1180610, whole genome shotgun sequence.
ACCESSION   DASTYP010000175 DASTYP010000000
VERSION     DASTYP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16601
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180610"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFV59_07910"
     CDS             <1..203
                     /locus_tag="VFV59_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325794.1"
                     /translation="ARAQPQRRAVAGEIVPMKAVKVLLISIAALVAAMIVIPLTILAG
                     LFVWLKLTEETDDEEMELDDAV"
     gene            complement(424..1254)
                     /locus_tag="VFV59_07915"
     CDS             complement(424..1254)
                     /locus_tag="VFV59_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEU5325795.1"
                     /translation="MTLAGRLWAAVRLVHPAPTLAVTTLSAALGAVLLNQAGRPLDDR
                     WLATVAAVLGSQIFTGATNDLVDRSRDQAAARLEKPLAAGDISPEGALWVASLGLGLQ
                     LAASLRIGELALVLGLVAVASALAYNVWLSRTPWSVVPYLVSFGVLPLWVAAGVGVPL
                     ERVAVAPLLVGPFAAAAHLANTLRDFEGDQATGSRNLAQLLGRTTSFRVAWVLAMAVG
                     LGVGVAFALGGRVELVGGALGVVGLIAVAQGYAGSDRLWGGMLVAAVCWTAAWALGSG
                     "
     gene            complement(1262..1540)
                     /locus_tag="VFV59_07920"
     CDS             complement(1262..1540)
                     /locus_tag="VFV59_07920"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEU5325796.1"
                     /translation="MTKRDPHVHLRHAFVRDGRLTSLPTRRPMLLAACAFLVDRFEPG
                     RAYPERVVNELLAADAPDHATLRRLLVDEAFLRREAGSYWRVEPDGPE"
     gene            1678..3384
                     /locus_tag="VFV59_07925"
     CDS             1678..3384
                     /locus_tag="VFV59_07925"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5325797.1"
                     /translation="MPKYVFVTGGVTSSLGKGITAASIGRILKARGIPVSILKLDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSQASNVTTGRIYQSVIAKE
                     RRGDYLGGTVQVIPHITNEIKERIARVAREGTGSVATSGLAAHGVVIVEVGGTVGDIE
                     SLPFLEAIRQMRKDVGRKNVLYVHVTWLPSIGATGELKTKPTQHSVSTLRGIGIQPDV
                     IILRSDEPIDDDILDKVSLFTDVDVHAVIPLKTARSIYEVPLQLERAGLGKLVSRELE
                     LPGGEPDLAEWQELVSRIRAPRPELEVAIVGKYTELPDAYISVTEALRHAALHHKVEV
                     KVRWIRSEHLERVEPDQVAQELDGVAGVLVPGGFGYRGVEGKVRAIRWARRNRIPYLG
                     LCLGLQAAVIEFAREALGTEDANSSEFNVFTEHPVIDLMPDQADVTDKGGTMRLGLYP
                     ARLDEGSLVREAYGSEIAYERHRHRFEVNNAYRERLGEAGMRFSGVSPDGRLVEYIEL
                     RDHPWFVATQAHPELKSRPNRPHPLFRDFVGAAAAHAGLRAEPIEVAGPTSPARSRVK
                     SS"
     gene            3385..4503
                     /locus_tag="VFV59_07930"
     CDS             3385..4503
                     /locus_tag="VFV59_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325798.1"
                     /translation="MSAQATRTDSDIDQRVAAAEAQAAVMWNNEPIAFADLPERIART
                     DRRALRDRLLAGYIEALDALEPMHRERLAAWLADGSPQPGEGDPVAMAEQMEPFALLS
                     ETWYFAALRRYLALIDIEQGDATIADLWHVLRGGAWEHWFGTRETREAVIATGREQIA
                     VSDVAGWRRAQEMLAGTPEPGEGVVRAAVRQAYGLLAGSREWLVRELRVPEHEVVPFV
                     DFAAFARLAELRRAFAVLLFEQRLYDDPDPGVSRAYYAGITGHMLGVQVPEAAYLHAV
                     PRPWASLEELRAALLAGDLVEVLEGRFGDAWWREAAAADLVASVRKASSVEDALAQLG
                     YDALDWRPVLRQIRTRLIGEMSGYGGPNITTRAGTRKV"
     gene            4511..4714
                     /locus_tag="VFV59_07935"
     CDS             4511..4714
                     /locus_tag="VFV59_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325799.1"
                     /translation="MTLRDPEHSRPIDPREQDARVDLFNCRSCRRFTNTLIPGRGWCS
                     RDGSPKLIEVRSYMKHCWQARRP"
     gene            4891..6180
                     /gene="rho"
                     /locus_tag="VFV59_07940"
     CDS             4891..6180
                     /gene="rho"
                     /locus_tag="VFV59_07940"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5325800.1"
                     /translation="MNLAELQAKPLEELRELAGEHDIENYEELEHSDLVLKLLDVVPL
                     APAQPQRNGQQPGTAEGVLEIVDEGFGFLRVNGVMPSSEDIYVSSSQVRRFGLRTGDQ
                     VTGQTRAPKDQEKYWGLLRVDTVNGVEPEVAKRRPYFDSLVPVFPDEMFDIETDQKNL
                     TQRLINLISPIGKGQRGLILSPAKAGKTTVLKQIAAGITENYPDTLLLVALIGERPEE
                     VTDMERSVKGEIYSSTFDEPTENHTRVAEMCLEIAKRQVETGRDVVILLDSITRLARA
                     YNLALPASGKTLSGGVDPVALYPPKRFFGAARNIENGGSLTIIATCLVDTGSRMDDVI
                     YEEFKGTGNMELALDRKLSEKRIFPAIDVLRSGTRREELLLDENSLRMVWTMRRMLSA
                     VGPNEGIELLMQRLGKTKNNAEFLVSLTKSVEASERS"
     gene            6207..7577
                     /locus_tag="VFV59_07945"
     CDS             6207..7577
                     /locus_tag="VFV59_07945"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5325801.1"
                     /translation="MTRVHRFALLALVLVLGACGADAPAPTSGADTPSLAPASVASAL
                     PSAPIVGEPEPAPGSDSTVYEPNPAAIVVAIDPGHGGCLDWGVPDPSERGVEWSEKQL
                     TLDIGLRLRDLLEAEGITVVMTRDADMALAGDDYPDLGCTGPAWRDVNGDGESGFDPE
                     GHTRTRDELQARLDLANLARADALVSIHINSPFDAGQSIAVAFTQTYYTDETPWGATA
                     TADLAADVQSGVVDALDEIAAYERGDRGTTAHNFYIVAPPLYEATEERPNPWAQPTRG
                     GLMPVILSEVGSITLRDEHDLLASDEGQAAVAEGILQGLRAWFGQRALAARIGLADEA
                     VGATPEAVAGDGPPFWATVVDPATPLRLRLTNTGTDAWPAGTALVAGWEPSDAPYLRQ
                     APEALDTLEVEVPSLEPGASVELEVVLPPVPAGGRALAWISLSDGLATFADFGSPALQ
                     VSTTRP"
     gene            7789..8574
                     /locus_tag="VFV59_07950"
     CDS             7789..8574
                     /locus_tag="VFV59_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5325802.1"
                     /translation="MPDPRRPFRRLVESTLPTRLIAHLTVVTLVVAASLVGISQADGS
                     GSSTAIDETAFSLVSQVRGAADATQPQAPSSLEVNPLIEEGATLDRGARAVVPELQPL
                     PTPVAVDPDATPLAAPDSATGTTATASSGTATTRGPAPRAAGALVWPVPGGTISQYYH
                     AGHLALDIAAPYGSTVVAAQAGVVTWSGWRNNGGGYVIQIDHGNGMQTVYNHLAGLWV
                     SAGQYVAAGQAIAPVGMTGIATGPHVHFEVIVNGVIDNPLRYF"
     gene            8639..9922
                     /gene="obgE"
                     /locus_tag="VFV59_07955"
     CDS             8639..9922
                     /gene="obgE"
                     /locus_tag="VFV59_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU5325803.1"
                     /translation="MFADRARIHVAAGAGGDGASTFRREAHVPRGGPDGGDGGNGGDV
                     ILQVEPGMTTLGDFKRHRHFRAAGGGRGMGRKAHGRNGTDTVIRVPPGTVVRTHPDGA
                     FVGELMQGDARLVVARGGRGGRGNVHFASATHRAPRHAEKGLPGEERWIELELKLIAD
                     IGLVGAPNAGKSTLLAALTEARPQVGDYPFTTVTPNLGVIWLDEERTAVIADVPGLIE
                     GAHEGRGLGHHFLRHVERTRVLVGVVDGAAREPIEEWRAVEDELRHHDASLLDRPMPI
                     VVTKLDLPEVRERWPDLRRQLAAGGSMEPIGVSAHDGTGLERLRARLATALDEAEELA
                     LERPATDEAVRVHRFDPLAEGWEVVAEADGFRVRGRRIERAAARTDFENDESRDRFWR
                     MLERAGIDMALRRAGAGPGDTVRIGATELEWGDEE"
     gene            9919..10635
                     /gene="nadD"
                     /locus_tag="VFV59_07960"
     CDS             9919..10635
                     /gene="nadD"
                     /locus_tag="VFV59_07960"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU5325804.1"
                     /translation="MSTERVGHGAAEGSLVTDLETELAPGAVEDGPHRIGILGGTFDP
                     PHVGHLWLATLAADSLDLDRVLFMPAAQPPHKRDRPISRASDRLLMTRLAIAGDPLFG
                     ISGIEMERPGLSYTIDTVAELEQLYGEGVTLYLVMAADSLNAIDTWRQPDALLARIEW
                     IVGPRPGNPMPDRSALRQRFGELAERIHLLEGPSLDVSSSEIRERVAARRAIRYLVPR
                     GVEEVIVDRRLYRTHKGTAS"
     gene            10650..11006
                     /gene="rsfS"
                     /locus_tag="VFV59_07965"
     CDS             10650..11006
                     /gene="rsfS"
                     /locus_tag="VFV59_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5325805.1"
                     /translation="MEIPSESAQLAHRIVEIASDKKGNDIVMLRTAELTTMADFFVIC
                     SGRSDRQVQALAGAIVEELREEGIRPIGVEGLSSSRWVLIDFGSVIVHVFAPEEREFY
                     GLERLWGKAAQVVRVV"
     gene            11096..11365
                     /locus_tag="VFV59_07970"
     CDS             11096..11365
                     /locus_tag="VFV59_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325806.1"
                     /translation="MTDRPRRSPPIETCRFCSGALLASVFDTVFRDAEADERLFFAIP
                     GALCVPCRQLYVDQQLIGILGLGGARCTFAIESDRVLMAGQAGAA"
     gene            complement(11390..12418)
                     /locus_tag="VFV59_07975"
     CDS             complement(11390..12418)
                     /locus_tag="VFV59_07975"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325807.1"
                     /translation="MTASLLLLHGDDGFAIDVALRDFARQIGADDRAEIVPERSPDEA
                     AIDRARLEAGSVGMFGAHLAVLRRPLYAAGRSTSAADRLVALVTELPDGSALALAEER
                     PSRDLAKPPALLKRLADAVRERGGRIEQHDAPRRGELRGWIVSHAARLGVPIEVRAAH
                     VLAERIGGAVWETDIERGEMTRVADSELRKLATYAGDRPISPDDVEALTADTRPASVF
                     AITNAVDRREPAAAAEALRRALAEGQPGLRILASLAGRVSDFIVARDLVAARTPPTEI
                     ARRLARGNARMAERLVDAAKRYPGDELDRMLIGLWEADVAIKSNVMDEEPALVAWIGE
                     HLLATARR"
     gene            complement(12415..12564)
                     /locus_tag="VFV59_07980"
     CDS             complement(12415..12564)
                     /locus_tag="VFV59_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325808.1"
                     /translation="MKRRHPKYAESIDAARHWAHVLEAQLAEAVELSVDEVVGALRDA
                     WERGR"
     gene            complement(12561..15440)
                     /locus_tag="VFV59_07985"
     CDS             complement(12561..15440)
                     /locus_tag="VFV59_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU5325809.1"
                     /translation="MESHAGRWFLPALLAGLCGGILAADAGVTVTVLAAPIGVLLAIP
                     GRRWLVPLTVAAALAGLGIGTVRALPAPPVPGSGVARAAALADGAERTFVGTVSDDPR
                     PREDRLQLVVGGVATTIDGSTATRLADRLLVWVPRGVDLSNGDRIRFSAELTLPRDFD
                     GFAYREYLERQGIGAIARPFAVEVIGREGGVGPAVGALRGMLLEGLNRLVPEPEAALG
                     AGILLGVRGSIDPDLNDAFARAGLTHVVAISGWNIAIVTAIVMGLLRPLERRPGGRWT
                     VTGIAVALVAGYVLLTGASPSVVRAALMAGCLLAAKLGGSRTHAASALGLAAMLMLLA
                     APSVLWDVGFQLSLLATAGLVWFGAAVESRVRWLPPLVREPIALTLAAQLTTLPIILV
                     NFERLSLVAPIANVLVVPFVPLAMATSAAAALAGTLVGVIPIAPVAEAVGWLGGAAAW
                     ACLRIIIALGSAVASVPNAAVDVRVPGAVAMAWLPLLGLAAWALGGAARAPATESSPR
                     RDAMPGGVRRLARPAPLLTCAMGVLLGITLLTGPDGRLHVTVLDIGQGDAILVEAPSG
                     ARMLVDGGPDPERTLRRLGAELPFHARRIDVLVVSHPHQDHVAGLVDVLDRFDVGLVL
                     HAGIPFENPAYDRLLADAARSTVPVRVARAGDRLALDAQTSVEVLYPSADEAAAPLPE
                     EDINNGSIVLLVRYGGFAALLTGDAEAPVEERLLAAGRVGPVDVLKVGHHGSTSSTTA
                     SFLDATAPAVAVISVGADNDYGHPAPETLETLTTRPGLLILRTDLDGDVEVETDGRVL
                     RVGTDHGWSETRPVHADGVTGSIGPWPFPIARLPTDCSTRPGCPTGSSFTARASRASP
                     WQGRRWSRRPGSRSTSRWSRRRRCCTTPTSSTSAKTVASTGSLARDGSRRSATPSSPC
                     RSPPIRSPRSSTRTAFPSAGRPCWWRSPTGTWRRSSSRSTSAWTT"
     gene            complement(15444..16112)
                     /locus_tag="VFV59_07990"
     CDS             complement(15444..16112)
                     /locus_tag="VFV59_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEU5325810.1"
                     /translation="MPRSDRLLIAAIGSALLLAVAAGWMLLAPTSAGLAAPSTDPDTA
                     FVVGSSAPTPLPSATGAAGGPGEGEIVVDVQGAVAEPGIRRLPGGARVADAIAAAGGY
                     ATDADLEAASVAINLAQPLVDGQQLRVPRIGEGVAAGSGGPADGPAASGQTGEPGGGG
                     LVDLNTATPEELEALPGIGPVTVQKLVAARQERPFASLQDAVDRGILNRGQLEQIQGL
                     ATAG"
     gene            complement(16288..>16601)
                     /locus_tag="VFV59_07995"
     CDS             complement(16288..>16601)
                     /locus_tag="VFV59_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5325811.1"
                     /translation="LMAPVAPHIAEELWSRRGKPYSIHQQPWPVADPEVAAADTIELP
                     VQVDGKLRDRLVVTPDTPAEEIERMVLASEHVQRYLAGRPPRRVIQIPGKLVNVVTPR
                     D"
BASE COUNT         2447 a         6067 c         5885 g         2202 t
ORIGIN      
        1 cagcccgcgc ccaaccccag cggcgcgccg tagcagggga gatcgttccc atgaaggcgg
       61 tcaaggttct actcatcagc atcgcggccc tggtggcggc gatgatcgtg atcccgctca
      121 ccatcctcgc ggggctcttc gtgtggctga agctcaccga ggagaccgat gacgaggaga
      181 tggagctcga cgacgccgtc tgacgcgtcc gtcgcatctc ttcgcgcctg aagcggtccc
      241 gattccgggg ccgcttcttg gtgtccagca cgtcgcgcct cgctcgtgtg aggagcattg
      301 cgtccggcga aggtcggttc gccggaccgg tcgctcccct gaggagcgcc gcacgagtgg
      361 gagcacgccc gccgggtctt ccgacggacg ctgtgctccc gtcaggagcg gtaacgacga
      421 cgcctacccg ctccccagcg cccaggccgc cgtccagcac acggccgcga ccagcatccc
      481 gccccacagc cggtcgctcc ccgcataccc ctgcgccacg gcgatcagcc cgacgacgcc
      541 gagcgcccca ccgaccagct cgaccctccc accgagggcg aaggcgacgc ccacccccag
      601 accgacggcc atggcgagca cccacgccac ccggaacgac gtcgtccgtc ccagcagctg
      661 ggccaggttc cgtgaaccgg tcgcctgatc gccctcgaag tcgcgcaggg tgttcgccag
      721 gtgcgcggcg gcggcgaacg gccccaccag cagcggggcc acggccacgc gctccagcgg
      781 aacgccgacg cccgccgcga cccacagcgg cagcacgccg aagctcacga ggtaggggac
      841 gaccgaccac ggcgttcgcg acagccagac gttgtacgcc agcgccgatg cgacggcgac
      901 cagccccagc accagcgcca gctcgccgat ccggagcgag gcggccaact gcagcccgag
      961 tcccagcgac gcgacccaca gcgctccctc cggggagatg tcgccggccg ccagcggctt
     1021 ctccaggcgc gccgccgcct ggtcgcggga tcggtcgacc agatcgttcg tggcgccggt
     1081 gaagatctgc gaccccagga ccgcggcgac ggtcgccagc cagcggtcgt cgagcggccg
     1141 tccagcctgg ttcaggagca ctgcgccgag cgccgcggac agcgtcgtga cggccagcgt
     1201 cggggcggga tggaccaatc ggaccgcggc ccacaggcgg ccggcgagcg tcatgcgccc
     1261 gtcactccgg cccgtctggt tccacgcgcc agtaggagcc ggcctcgcgt cgcaggaatg
     1321 cctcgtcgac cagcagccgg cgcagggtgg cgtggtccgg cgcatcggcg gcgagcagct
     1381 cgttcaccac acgttcgggg tacgcgcgtc cgggctcgaa gcgatcgacg aggaaggcgc
     1441 aggcggccag gagcatcggc ctcctcgtcg ggagcgaggt caggcggccg tcgcgcacga
     1501 aggcatggcg caggtggacg tgcggatcgc gcttcgtcat cggcgcggac cgtacaccca
     1561 accgcggctc ggtgcggtat gatcggttcc cgagtggcgc aaggcgacgc tgctcgcgcg
     1621 cggcccactc gtcgctcagc cgcccgctcg agccacccgc gaggtgcaca gcaaccgatg
     1681 ccgaagtacg tcttcgtcac gggcggagtc acgagctcac tcggcaaggg catcacggcc
     1741 gccagcatcg ggcgcatcct gaaggcccgc ggcatcccgg tcagcatcct gaagctcgac
     1801 ccgtacatca acatcgaccc cggcaccatg tcgccgtacc agcacggcga ggtgttcgtc
     1861 accgatgacg gcgccgagac cgatctcgac ctcggccact acgagcgctt catcgacgag
     1921 aacctgagcc aggcgagcaa cgtcaccacg ggacgcatct accagagcgt catcgccaag
     1981 gagcgccgcg gcgactacct gggcggcacg gtgcaggtca tcccgcacat caccaacgag
     2041 atcaaggagc gcatcgcgcg cgtggcacgc gaggggaccg gcagcgtcgc caccagcgga
     2101 ctcgcggccc atggcgtggt catcgtcgag gtgggtggga ccgtgggtga catcgagtcc
     2161 ctgccgttcc tcgaggcgat ccgccagatg cgcaaggacg tcgggcgcaa gaacgtcctg
     2221 tacgtccacg tcacctggct gccgtccatc ggcgccaccg gcgagctgaa gaccaagccg
     2281 acgcagcact cggtcagcac gctgcgtggc atcgggatcc aacccgacgt catcatcctg
     2341 cgcagcgacg agccgatcga cgacgacatc ctggacaagg tcagcctctt caccgatgtg
     2401 gacgtccacg ccgtgatccc gctcaagacc gcccgctcga tctacgaggt cccgctgcag
     2461 ctggagcgtg ccgggctcgg caagctggtc tcccgcgagc tggagctgcc aggcggcgag
     2521 ccggacctgg cggagtggca ggagctcgtc agccgtatcc gcgccccacg gccggagctc
     2581 gaggtcgcca tcgtcggcaa gtacaccgag ctgccggacg cgtacatcag cgtcaccgag
     2641 gcgctgcgcc acgccgcgct ccaccacaag gtcgaggtga aggttcgctg gatccgctcc
     2701 gagcacctcg agcgcgtcga gccggaccag gtggcccagg agctcgacgg cgtcgccggc
     2761 gtgctggtgc cgggtggctt cggctaccgc ggcgtcgagg gcaaggtccg cgccatccgc
     2821 tgggcgcgcc gcaaccggat tccgtacctg ggcctgtgcc tgggactcca ggccgcggtc
     2881 atcgagttcg cccgcgaggc gctgggcacg gaagatgcga actccagcga gttcaacgtg
     2941 ttcaccgagc atcccgtcat cgacctcatg ccggaccagg ccgacgtcac cgacaagggc
     3001 ggcaccatgc gtctggggct ctacccggcg cggctggatg aggggtcgct ggtgcgcgag
     3061 gcctacgggt cggagatcgc gtatgagcgc caccgccatc ggttcgaggt gaacaacgcc
     3121 taccgtgagc gcctgggcga ggcggggatg cggttcagcg gcgtctcgcc cgacgggcgc
     3181 ctggtcgagt acattgagct gcgcgaccac ccgtggttcg tggcgacgca ggctcacccg
     3241 gagctgaaga gccgtccgaa ccggccgcac ccactcttcc gcgacttcgt cggtgccgcc
     3301 gccgcccatg ccggtctgcg cgccgaaccc atcgaggtcg ccggaccgac ctcccccgca
     3361 cgcagcaggg tcaagagcag ctgaatgagc gcccaggcca ccaggaccga ttccgacatc
     3421 gaccagcgcg tcgccgccgc cgaggcccag gcggcggtga tgtggaacaa cgagccgatc
     3481 gccttcgctg acctgccgga gcggatcgcg cggaccgatc gtcgcgccct gcgcgaccgg
     3541 ctgctggccg gatacatcga ggcgctggat gccctggagc cgatgcaccg cgaacgcctg
     3601 gccgcctggc tggccgacgg gagcccgcag cccggtgagg gcgacccggt cgccatggcc
     3661 gagcagatgg agccgttcgc cctgctctcg gagacgtggt acttcgcggc gctgcgccgc
     3721 tacctggcgc tgatcgacat cgagcagggt gacgcgacca tcgccgacct gtggcacgtg
     3781 ctgcgcggcg gcgcgtggga gcactggttc ggcacgcgag agacgcggga ggcagtcatc
     3841 gccacggggc gcgaacagat cgccgtgtcc gacgtcgcgg gctggcgccg cgcgcaggag
     3901 atgctggcgg gaacgccgga gcccggtgag ggcgtggtgc gcgccgcggt acggcaggcg
     3961 tacggcctcc tcgccggcag ccgagagtgg ctggttcgcg agctgcgcgt ccccgagcac
     4021 gaggtggtgc ccttcgtgga cttcgccgcc ttcgcgcgcc tcgccgagct gcgacgcgcc
     4081 ttcgccgtgc tcctgttcga gcaacgcctc tacgacgatc cggatcccgg tgtgtcgcgg
     4141 gcgtactacg ccggcatcac cggtcacatg ctgggcgtcc aggtgcccga ggccgcgtac
     4201 ctgcacgccg tgccgcggcc atgggcgtcg ctggaggagc tgcgggcggc actcttggcc
     4261 ggcgacctgg tggaggtgct cgagggtcgg ttcggtgacg cctggtggcg cgaggctgcg
     4321 gcggcggacc ttgtcgcatc ggtgcgcaaa gcgtccagcg tcgaggacgc tcttgcgcaa
     4381 ctcgggtatg atgcgctcga ctggagaccc gtcctgcgtc agatccggac gcggctcatc
     4441 ggtgagatga gcgggtacgg cgggcccaac atcaccaccc gtgccgggac ccgcaaggtc
     4501 tgatccgttc ttgaccctac gcgatcccga gcactccagg ccgatcgatc cacgggagca
     4561 ggacgcccgg gtcgacctct tcaactgccg atcctgccgc cgcttcacca acaccctcat
     4621 cccgggccgt gggtggtgca gcagggacgg ctccccgaag ctcatcgagg tcaggtccta
     4681 catgaagcac tgctggcagg cgaggcgacc atgaacgacg acgcacgacc gcagcagcag
     4741 cgtcggcgtt cccgacgccg ccaagacaac gcgcctcgcc gtcagtcgca gcgcggcggc
     4801 ttccagccgc gccgtcgcaa cgactacgcg gccggccagt actcgtccat ctacacgggc
     4861 ccgttcccga ccgatgctga cgagccggcc ctgaacctcg cggagctgca ggcgaagccg
     4921 ctcgaggagc tgcgcgagct cgccggcgag cacgacatcg agaactacga ggagctcgag
     4981 cactcggacc tcgtgctgaa gctgctcgac gtcgtgccgc tcgcccctgc ccagcctcag
     5041 cgcaacgggc agcagccggg caccgccgag ggcgtgctcg agatcgtgga cgagggcttc
     5101 ggcttcctgc gcgtcaacgg cgtcatgccg tcctcggagg acatctacgt cagctcgtcc
     5161 caggtccggc gcttcgggct gcgcaccggc gaccaggtga ccggccagac ccgcgccccc
     5221 aaggaccagg agaagtactg gggcctgctg cgcgtcgaca ccgtcaacgg cgtcgagccg
     5281 gaggtcgcca agcggcgccc gtacttcgat tcgctcgtgc cggtcttccc ggacgagatg
     5341 ttcgatatcg agaccgacca gaagaacctg acccagcgcc tgatcaacct gatcagtccc
     5401 atcggcaagg gccagcgcgg cctgatcctg tcgccggcca aggccggcaa gacgaccgtc
     5461 ctcaagcaga tcgccgccgg gatcaccgag aactaccccg acaccctcct cctcgtggcc
     5521 ctcatcggcg agcggccgga ggaggtgacc gacatggagc gctcggtcaa gggcgagatc
     5581 tattcgagca ccttcgacga gccgacggag aaccacaccc gagtggcgga gatgtgcctg
     5641 gagatcgcca agcgccaggt cgagaccggc cgggacgtgg tgatcctgct cgactccatc
     5701 acgcgcctgg cgcgcgccta caacctggcc ctgccggcgt cgggcaagac cctgtccggc
     5761 ggcgtggacc cggtggcgct gtacccgccg aagcgcttct tcggtgccgc gcgcaacatc
     5821 gagaacgggg gctccctgac gatcatcgcc acctgcctgg tcgacaccgg cagccgcatg
     5881 gacgacgtga tctacgagga gttcaagggc accggcaaca tggagctggc cctcgaccgc
     5941 aagctcagcg agaagcggat cttcccggcc atcgacgtgc tgcgctccgg cacccggcgc
     6001 gaggagctgc tcctcgacga gaactccctg cggatggtgt ggaccatgcg gcgcatgctc
     6061 agcgcggtcg ggcccaacga ggggatcgag ctgctgatgc agcgcctggg caagaccaag
     6121 aacaacgccg agttcctggt ctccctgacg aagtcggtcg aggcgtccga gcgctcctga
     6181 ccgatcctgg cacccgacct cccgtcgtga cccgcgtgca ccggttcgcg ctcctggcgc
     6241 tggtgctggt gctcggcgcc tgcggggccg atgcgccggc ccccacgtcc ggcgctgaca
     6301 ccccgtccct ggcgccggcg tcggtcgcct ctgccctgcc ctccgcgccg atcgtcggcg
     6361 agccggagcc tgcaccgggc tccgacagca cggtctacga gccgaacccc gccgccatcg
     6421 tggtcgccat cgacccgggc cacggcggat gcctcgactg gggggttccc gatccctcgg
     6481 agcggggcgt cgagtggtcc gagaagcagc tgaccctcga catcgggctg cgcctgcgcg
     6541 acctgctgga ggcagagggc atcaccgtgg tgatgacgcg ggacgccgac atggcgctgg
     6601 cgggggacga ctaccccgac ctgggctgca ccggaccggc atggcgcgac gtgaacggcg
     6661 acggggagtc cggcttcgat ccggagggcc acacccggac ccgcgacgag ctgcaggccc
     6721 ggctggacct cgccaacctg gcccgggcgg atgcgctcgt cagcatccac atcaactcgc
     6781 cgttcgacgc cggccagtcg atcgccgtcg ccttcacgca gacctactac accgatgaga
     6841 ccccttgggg cgccacggcc accgccgacc ttgccgccga cgtgcagtcg ggcgtcgtgg
     6901 acgcgctgga cgagatcgcc gcctacgagc gcggggaccg cgggacgacg gcgcacaact
     6961 tctacatcgt ggcgccgccg ctgtacgagg cgacggagga acgcccgaac ccgtgggccc
     7021 agcccacgcg gggcggcctg atgcccgtca tcctgagcga ggtgggctcg atcacgctgc
     7081 gcgacgagca cgatctgctc gcctcggacg aggggcaggc tgccgtcgcc gagggcatcc
     7141 tccaggggct gcgggcgtgg ttcggccagc gcgcgctggc cgcgcgcatc ggcctggcgg
     7201 acgaagcggt cggggcgacg ccggaggcgg tcgccggcga cggacccccc ttctgggcca
     7261 cggtcgtcga tccggcgaca ccgcttcggc tgcggctcac gaacaccggc accgacgcat
     7321 ggcccgccgg caccgcgctg gtggccggct gggagccgag cgacgcgccg tacctccggc
     7381 aggcgccgga ggcgctcgac acgctggagg tcgaggtgcc gtcgctggag cccggcgcat
     7441 cggtcgagct ggaggtcgtg ctcccgcccg tgcccgccgg cggacgcgcg ctggcatgga
     7501 tttcgctatc ggatggtctc gcgaccttcg ccgacttcgg gtctcccgcg ttgcaggtgt
     7561 ccaccacgag accctgaacc ggatcggtcc tcaccggacc gacaaccccg gaggtaccgg
     7621 tcgtcgctgc gcgttttcgg ttgcggctcc cccgtccgca ctgctaggat gcccgcagca
     7681 acggaaccgg ccacggccga ccgcgagccc atcacgagtc ggggcccacc aggcggtttc
     7741 gctgttcagc gatgcaaaca accgtagtag gcggggcctg accttctatt gcctgatcca
     7801 cgacgaccgt tccgccggct ggtcgagagc acccttccga cgcgcctgat cgcgcacctc
     7861 accgtcgtca ccctggtggt ggcggcttcg ctcgtcggaa tctcccaggc cgatggcagt
     7921 ggcagctcga ctgccatcga tgagaccgcc ttctcgctcg tgtcccaggt ccggggcgcg
     7981 gccgacgcca cccagccaca ggctccgagc tccctcgagg tcaacccgct gatcgaggaa
     8041 ggagccacgc tcgaccgggg cgcccgcgcg gtcgttcccg agctccagcc cctgccgacc
     8101 ccggtggcgg tggatcccga cgccacgcct ctcgccgcac cggattcggc gaccggcacc
     8161 acggccaccg cgtcgagcgg gaccgccacc acccgcggcc cggcgccgcg agccgccggc
     8221 gcgctggtgt ggccggtgcc cggcgggacc atcagccagt actaccacgc cggtcatctc
     8281 gccctggaca tcgccgcccc ctacggttcg acggtggtcg ccgcccaggc cggtgtcgtg
     8341 acctggtcgg gctggcgaaa caacggcggc ggctacgtca tccagatcga ccacggcaac
     8401 ggcatgcaga cggtgtacaa ccacctggcc ggcctatggg tctccgcggg ccagtacgtc
     8461 gccgccggac aggcgatcgc gccggtcggc atgacgggta tcgcgaccgg gccgcacgtc
     8521 catttcgagg tcattgtcaa cggggtcatc gacaacccgc tgcgctactt ctgatcctcg
     8581 ctccgtcacc cacgcgaacg ccggcgctcg acgccggcgt tgctatgctc aggccctgat
     8641 gttcgccgat cgagcccgga tccacgtcgc ggccggtgcg ggtggtgacg gcgccagcac
     8701 cttccgtcgt gaagcccacg tcccgcgtgg cggcccggac ggcggcgacg gcggcaacgg
     8761 cggcgacgtc atcctgcagg tcgagccagg catgacgacg ctgggcgact tcaagcgcca
     8821 ccgccacttc cgcgccgccg gtggagggcg ggggatgggc cgcaaggccc acggccgcaa
     8881 cggcacggat accgtcatcc gcgtcccgcc cggcacggtc gtgcgcaccc atcccgatgg
     8941 cgcgttcgtc ggggagctaa tgcagggcga tgcccgcctg gtggtggccc gtggtgggcg
     9001 cggcggtcgc ggcaacgtcc acttcgcatc ggccacgcac cgtgccccgc gccatgccga
     9061 gaagggcctg cccggggagg agcgctggat cgagctggag ctgaagctga tcgcggacat
     9121 cgggctcgtc ggcgcgccga acgcgggcaa gtcgacgttg ctcgccgcgc tcaccgaggc
     9181 acgcccccag gtcggcgact acccgttcac caccgtgacg ccgaacctcg gcgtgatctg
     9241 gctcgacgag gaacggaccg cggtcatcgc cgacgtcccg ggtctgatcg agggcgcgca
     9301 cgaggggcgg ggcctcgggc accatttcct gcgccacgtg gagcgcaccc gcgtcctggt
     9361 cggggtggtc gacggggccg ctcgcgagcc gatcgaggag tggcgcgccg tcgaggacga
     9421 gctccgccac cacgacgcct cgctgctgga tcgcccgatg cccatcgtcg tcaccaagct
     9481 cgacctgccc gaggtgcgcg agcggtggcc ggacctgcgg cggcagctgg ccgccggcgg
     9541 ctcgatggag cccatcggcg tgagcgcgca cgacggcacc ggcctggagc ggcttcgcgc
     9601 gcgcctcgcc accgccctcg acgaggcgga ggagctcgcg ctggagcggc cggccaccga
     9661 cgaggcggtg cgggtgcatc ggttcgatcc gctggcggag ggctgggagg tcgtcgccga
     9721 ggctgatggc ttccgggttc gaggccgccg gatcgagcgc gccgccgcgc ggaccgactt
     9781 cgagaacgac gagtcccggg atcgcttctg gcgcatgctc gagcgtgccg ggatcgacat
     9841 ggcgctgcgg cgggcggggg cgggacctgg cgacacggtc cgcatcggcg cgaccgagct
     9901 ggagtggggc gacgaggagt gagcaccgag cgggtcgggc acggggcggc ggaggggagc
     9961 ctggtcaccg acctggagac ggagctcgct cccggcgcgg tggaggatgg tccacatcgg
    10021 atcggcatcc tgggcggcac cttcgatccg ccgcacgtcg ggcacctgtg gctcgcgacc
    10081 ctcgccgcgg acagcctcga tctcgaccgc gtgctgttca tgccggcggc gcagccgccg
    10141 cacaagcgcg acaggccgat cagccgcgcg tcggatcggc tgctgatgac gcgcctcgcg
    10201 atcgccggcg acccgctgtt cgggatctcg ggcatcgaga tggagcgacc cgggctctcg
    10261 tacaccatcg acacggtcgc cgagctcgag cagctgtacg gggagggcgt gaccctctac
    10321 ctcgtgatgg cggccgattc gctcaacgcc atcgacacct ggcggcagcc ggatgcgctg
    10381 ctggcgcgga tcgagtggat cgtggggccc cgccccggca acccgatgcc cgaccgctcc
    10441 gcgctgaggc agcgcttcgg cgagctggcg gagcggattc acctcctcga gggtccgtcg
    10501 ctggacgtga gcagctccga gatccgcgag cgggtggcag ccaggagggc catccggtac
    10561 cttgtgccgc gcggcgtcga agaggtgatc gtcgaccgcc ggctgtaccg cacgcacaag
    10621 gggaccgcat cctgaccgac gccgaccacg tggagattcc gtccgagagc gcgcagctgg
    10681 cgcatcggat cgtggagatc gcgtccgaca agaagggcaa cgacatcgtc atgctgcgga
    10741 ccgccgagct gacgaccatg gccgacttct tcgtcatctg ctcgggccgc agcgatcggc
    10801 aggtgcaggc gctcgccggc gccatcgtcg aggagctgcg tgaggagggg atccggccca
    10861 tcggcgtgga ggggttgagc tcgtcgcgct gggtgctgat cgacttcggg tcggtgatcg
    10921 tgcacgtctt cgcccccgag gagcgcgagt tctacgggct cgagcggctg tggggcaagg
    10981 ccgcccaggt cgtgcgcgtc gtctgaccct gcccgtccga agtactaggg gggtcctccc
    11041 gggctggccc atcggtccca ttgagggggt gcggtggggt tcgcatgctc gccgcatgac
    11101 cgaccgccca cgtcgctcgc cgccgatcga gacgtgccgc ttctgcagcg gcgccctcct
    11161 ggcgagcgtc ttcgacaccg tcttccgcga tgcggaggcc gatgagcgac tcttcttcgc
    11221 cattcccggc gcgctgtgcg tgccgtgccg ccagctgtac gtcgatcagc agctgatcgg
    11281 catcctgggc ctcggcgggg cgcgctgcac cttcgccatc gagtccgacc gggtgctcat
    11341 ggcgggccag gccggagccg cctgactccc tcccgttcgc cggtcgagcc tatcgccgcg
    11401 ccgtcgcgag caggtgctcg ccgatccacg ccaccagcgc cggctcctcg tccatgacgt
    11461 tcgacttgat cgccacgtcc gcctcccaca ggccgatcag catgcggtcg agttcgtcgc
    11521 cgggataccg cttcgcggcg tcgaccaggc gctcggccat ccgggcgttt cccctcgcca
    11581 ggcgccgggc gatctccgtg ggtggggtgc gggccgccac caggtcgcgc gccacgatga
    11641 agtcgctgac gcgtcccgcc agcgacgcga ggattcgcag accgggctgg ccctcggcca
    11701 gcgcccgtcg cagggcctcc gctgccgccg ccggctcgcg gcggtccacg gcgttggtga
    11761 tggcgaagac cgatgccggg cgcgtgtcgg ccgtcagcgc ctcgacgtcg tcggggctga
    11821 tcggccggtc gccggcgtag gtcgcgagct tgcggagctc ggagtcggcg accctggtca
    11881 tctcgccgcg ttcgatgtcc gtttcccaca ccgcaccgcc gatgcgctcg gccagcacgt
    11941 gcgcggctcg gacctcgatc gggacaccca ggcgcgccgc gtgcgacacg atccagccac
    12001 gcagctcgcc gcgccgcggg gcgtcgtgct gctcgatcct tccgccccgt tcgcgcaccg
    12061 cgtccgcgag gcgcttcagc agcgccggcg gcttggccag gtcgcgcgac ggccgctcct
    12121 cggcaagggc cagggccgag ccgtcgggga gctcggtcac cagcgccacc aggcggtcag
    12181 cggcggacgt cgagcggccg gcggcgtaga gcggccggcg caggacggcc aggtgcgcgc
    12241 cgaacatccc gaccgagccc gcctccagcc gcgcacgatc gatggccgcc tcgtccggcg
    12301 accgctccgg cacgatctcc gcgcggtcgt ccgccccgat ctggcgcgcg aagtcgcgca
    12361 gcgcgacgtc gatggcgaag ccgtcgtctc cgtgcaggag cagcagcgac gccgtcaccg
    12421 cccgcgttcc cacgcgtcgc gcagcgcacc caccacctcg tccacgctca gctcgacggc
    12481 ctccgccagc tgcgcctcca gcacgtgcgc ccagtggcgg gccgcatcga tgctctcggc
    12541 atacttcggg tgtctccgct tcatgtcgtc caggcgctcg tcgaccgtga ggaactcctg
    12601 cgccacgtgc cggtcggcga tcgccaccag cacggacggc cagccgatgg gaaagcggtc
    12661 ctggtcgagg agcgcggtga tcggatggga ggcgaccggc atggcgagct cggggtagcc
    12721 gagcgcctcg agccgtcgcg cgccaacgat cccgtgctcg ccaccgtctt cgcggacgtc
    12781 gagcttgtcg gtgtcgtgca gcagcgccgc cgcctcgacc agcgcgacgt cgaccgggat
    12841 cccggccgcc gcgaccatct gcgcccctgc cacggcgacg cgcgcgacgc cctcgctgtg
    12901 aacgacgatc ccgtcgggca gcccggcctc gtggagcagt cggtcggcag tcgcgcgatc
    12961 gggaacggcc atggaccgat gctacccgta acgccgtccg cgtggaccgg tcgggtctct
    13021 gaccagccgt ggtccgtgcc gacgcgcagg acgcgtccgt ccgtctcgac ctcgacgtca
    13081 ccgtccaggt cggtgcgcag gatcaggagc cccggccgcg tggtgagggt ctcgagcgtc
    13141 tccggcgccg ggtgcccgta gtcgttgtcg gctcccacgg agatcacggc gaccgcgggg
    13201 gcggtcgcgt cgaggaacga cgcggtggtc gaggacgtgg agccgtggtg gccgaccttc
    13261 agcacgtcga ccgggccgac ccggcccgca gccagcagcc gctcttcgac gggtgcttcg
    13321 gcgtcacccg tcagcagtgc ggcgaagccg ccgtagcgaa cgagcagcac gatcgagccg
    13381 ttgttgatgt cctcctccgg gaggggtgcg gctgcctcgt cggccgaggg gtacagcacc
    13441 tcgaccgagg tctgagcgtc gagcgccaga cgatcgccag cccgggcgac gcggaccggc
    13501 acggtcgagc gggcggcatc ggcgagcagg cggtcgtacg ccgggttctc gaacgggatg
    13561 ccggcgtgca gcacgaggcc gacgtcgaat cggtcgagca cgtcgacgag cccggcaacg
    13621 tggtcctggt gggggtggct gaccacgagc acgtcgatcc gacgcgcatg gaagggcagc
    13681 tccgcgccca gccggcgcag agtccgctcg ggatcgggcc cgccgtcgac cagcatcctg
    13741 gccccgctcg gcgcctccac gaggatggcg tcgccctgcc cgatgtccag gacggtcaca
    13801 tgcagccggc catccgggcc cgtgagcagc gtgatcccga ggaggacgcc catcgcgcac
    13861 gtcagcagcg gagccggtcg cgccaggcgt cgcacaccgc cgggcatggc atcacgccgt
    13921 ggcgacgact cggtggccgg ggcgcgggcc gcgcccccca gcgcccatgc ggcgaggccg
    13981 aggaggggga gccacgccat ggcgacggct ccgggaactc ggacgtcgac ggccgcgttc
    14041 ggaaccgatg cgacggccga cccgagcgcg atgatgatgc gcaggcacgc ccacgcggcc
    14101 gctcctccga gccagccgac ggcctccgcg acgggtgcga tcgggatgac gccgacgagc
    14161 gtgcccgcga gtgcggcggc ggcgctggtg gccatggcca gcggcacgaa cggaacgacc
    14221 agcacgttcg cgatcggcgc gaccagcgag aggcgctcga agttcacgag gatgatcggc
    14281 agggtcgtca gctgcgcggc cagcgtcagc gcgatcggct cccggaccag tggcggcagc
    14341 cagcgcacgc gcgactccac cgccgcgccg aaccacacga ggccggcggt cgccaggagc
    14401 gacagctgga acccgacatc ccacagcacc gatggcgccg ccagcagcat gagcatcgcc
    14461 gccagcccca gcgccgatgc cgcgtgggtc cgcgacccgc cgagcttcgc ggcgaggagg
    14521 cagccggcca tgagcgcggc ccgaacgacc gatgggctgg ctccggtcaa cagcacgtag
    14581 ccggcgacca gcgccacggc gatgccggtg accgtccatc gaccgcccgg ccgacgctcg
    14641 agagggcgga gcagccccat gacgatggcg gtcacgatcg cgatgttcca gccactgatc
    14701 gccacgacgt gggtgaggcc ggcccgcgcg aaggcgtcgt tgaggtcagg atcgatggag
    14761 ccgcggacgc cgagcaggat gccggccccc aatgccgcct ccggctccgg caccaggcgg
    14821 ttcaaccctt ccagcagcat gccgcgcaag gcaccgaccg ccggtcccac gccgccctcg
    14881 cggccgatca cctcgacggc gaacgggcgg gcgatggcgc cgatgccctg gcgctcgagg
    14941 tattcccggt acgcgaagcc gtcgaagtcc cgcggcaggg tcagctccgc gctgaagcgg
    15001 atgcgatcgc cgttcgatag gtcgacgccg cgcggcaccc agacgagcag ccggtccgcc
    15061 aggcgcgtgg cggtgctgcc gtcgatggtg gtggccacgc cgccgacgac gagctggagc
    15121 cgatcctcgc gtggacgcgg gtcgtcgctg acggtcccga cgaaggttcg ttcggcccca
    15181 tcggccagtg cagccgcgcg agcgacgccg gaccccggca cggggggagc ggggagtgcc
    15241 cgaaccgtcc cgatccccag gcccgcgaga gcggccgcga cggtcaacgg caccagccac
    15301 cggcgcccgg ggatggcgag caggacgccg atcggcgccg ccaggaccgt gacggtcacc
    15361 cccgcatccg cggcgaggat gccgccgcag aggccagcca gcagggcggg gaggaaccag
    15421 cgcccggcgt gcgactccac cgctcagccc gcggtggcca acccctggat ctgttcgagc
    15481 tgtccgcgat tgaggatgcc gcggtcgacg gcgtcctgca gtgacgcgaa cggccgttcc
    15541 tgtcgcgccg ccaccagctt ctgcacggtc accggaccga tgcccggcag cgcctccagc
    15601 tcctcgggcg tcgcggtgtt caggtcgacc aggcctccac cgccgggttc gcccgtctgg
    15661 cccgacgcgg ccggcccgtc cgcgggtcct ccagaacctg ccgccacgcc ctcaccgatg
    15721 cgcggcaccc gcagctgttg cccgtcgacc agcggctggg cgaggttgat ggcgaccgac
    15781 gccgcctcga ggtcggcgtc cgtggcatat cccccggcgg cggcgatggc gtccgcgacg
    15841 cgcgcaccgc ccggcagccg gcggatcccc ggctcggcca ccgcgccctg cacgtcgacg
    15901 acgatctcgc cctccccggg cccgcccgcc gcgccggtgg cgctggggag cggcgtcggg
    15961 gccgaggacc ccacgacgaa ggccgtgtcc gggtccgtgc tcggcgctgc caacccggcc
    16021 gacgtgggtg ccagcagcat ccagccggcg gcgacggcca gcagcagcgc ggagccgatg
    16081 gccgcaatga ggagtcggtc ggaacggggc acgatcggac gacctccgga gcggggaggc
    16141 gatccgggtg agtgggaacg agacgagcct gcgtccgaag cccggcgccc gggcactcga
    16201 cgccgggcct cggaagcagg gtgcgcagcc tacgccgcgc ggccttatcg gctcgttacc
    16261 gggggcttac tgccgagggc gaccgattca gtcgcgcggc gtcacgacgt tgacgagctt
    16321 gccggggatc tggatcacgc ggcgcggcgg ccggcccgcc aggtagcgct ggacgtgctc
    16381 cgaggcgagg accatgcgct cgatctcctc ggccggcgtg tcgggcgtga ccaccaggcg
    16441 gtcccgcagc ttgccgtcga cctggaccgg cagctcgatg gtgtccgccg ccgccacctc
    16501 cggatcggcc accggccagg gctgctggtg gatcgagtac ggcttgcccc ggcgcgacca
    16561 cagctcctcg gcgatgtgcg gcgccaccgg cgccatcagc a
//
LOCUS       DASTYP010000176         8484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_160271, whole genome shotgun sequence.
ACCESSION   DASTYP010000176 DASTYP010000000
VERSION     DASTYP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160271"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFV59_08000"
     CDS             <1..334
                     /locus_tag="VFV59_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HEU5325812.1"
                     /translation="DQRGDSSLDDVRRTTRLGMGPCQGAFCTPRAAAVLDERAAAGRL
                     APQAPAPNAAMRGFLQERFRGPRPIAWGEQLTELWLSAGIFRGLLAVDELPDTSSPLA
                     EDRVDAAG"
     gene            321..1577
                     /gene="glpB"
                     /locus_tag="VFV59_08005"
     CDS             321..1577
                     /gene="glpB"
                     /locus_tag="VFV59_08005"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpB"
                     /protein_id="HEU5325813.1"
                     /translation="MPRADVVVIGAGLAGLSCAAELAERGARVLLAAKGMATTHWTHG
                     GLDVAGIPGAATPRAGVEALRSNTLHPYAKLASDVGPAVAAHLRRTDAEGLPHAGDLD
                     ASLRPMPTAIGTLRPAAIVPAAQAPALAPWNGDGLLLVGIEHYRDAWAPWAARNLASA
                     LWPDGAPREVRGITVDLPWLDRLHNLNATVLARLFDDPVWRERTLGAIAQAVPAGSWR
                     IGLPAALGLERHREVHADAEAALGHPCFEVPSLPPSVPGMRLFEALRRRIIGAGGRIH
                     LGFEVVQVERDEQRVHAIHTHAGARTLRLLGDAFVLATGGLAGTGFRGRRDGTVEERI
                     FGLHVTAPPHRAWFSDDPLQPHPLEAAGIETDDALRPPELENVRVIGSALAGMHYLSE
                     RCGDGVALASAYRVAAELGGTARAAA"
     gene            1574..2875
                     /locus_tag="VFV59_08010"
     CDS             1574..2875
                     /locus_tag="VFV59_08010"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HEU5325814.1"
                     /translation="MTFHDAATRDFAPFSTDQCIKCTVCTTVCPVSRVTDAFPGPKYV
                     GPQAQRFRGPTVMEASPDVTVDWCSGCGYCTRSCPTGVRIAETNSRARAAMVVRRGSG
                     LRNRLISDTDLLIRLGVPVAPLANFGLRNRLLRWIGEKLVGVHRRGPLSPFARRTFRS
                     WWKRERRPLDLPPGADPHRTVIYFHGCAVNGFEPDLGRQAVEVLERNGFTVISPDQGC
                     CGLPFISNGLYDHARRKARRNVESFAELARAGVRIVGTSTSCTYAMKAEYHEMLDLDD
                     ADAHAVARATWDICELLVDLHDRGELDTSFGPMHEAVPYHPPCQLKSHGIGTPAMDLF
                     SLIPGFRATDSDHDCCGAAGTYGLKVEKWQIAQDVGAPLFAKVRAAVADGSRRAVCDS
                     ETCRWQIEQSSGVDTVHPITVLADAYRRGDEAAREPWRRVV"
     gene            complement(2885..3721)
                     /locus_tag="VFV59_08015"
     CDS             complement(2885..3721)
                     /locus_tag="VFV59_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325815.1"
                     /translation="MTAIAAEPRRSAPSIWATAWDRIWRGRRLVERNILVYRHQWIII
                     VSGVFEPIFYLLGIGLGIGGLVQTVPPELVGGREVSYVAFVAPALVATAAMNGAVFET
                     IFNIFFKLNYAKTYDGVLATPMGITEIAIGEMMWALMRAALYAVAMFVIMLVTGLILS
                     PWGLLVIPAALLVAAAFAAAGLAGTSYLRTVNDFDVPMGLIVQPMFLFSGTFFPITIY
                     PWFIQVFMQATPLYHSISLIRGLSLGMVGWQQVWDLAYLLAFFAVGMWIAIRQMERKL
                     IK"
     gene            complement(3726..4505)
                     /locus_tag="VFV59_08020"
     CDS             complement(3726..4505)
                     /locus_tag="VFV59_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325816.1"
                     /translation="MTVAAGSGRVWQRNWLVYKRLWHRSLFFGFLQPFLFLTAMGIGI
                     GTLIGGQSASAFGGVDYIDWLGPGLMAAMAMQTGTFESTYPIMNKIMWGRNYEAMLST
                     PLTVRGIIGGEFGWIAFRVGTLATIFLVVLAVFGIPRTPMAVLAIPVAVLVGLAFSAC
                     LIAFTATQKNDVGFSAIFRFVINPLFLFSGTFFPLTQLPEPIRAVAWATPLFHGVELI
                     RGSILDQLDPLTAPIHLAYLLAMFGIGAFLAERNLIKRMAS"
     gene            complement(4511..5446)
                     /locus_tag="VFV59_08025"
     CDS             complement(4511..5446)
                     /locus_tag="VFV59_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018815530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325817.1"
                     /translation="MTTPDSLVHARGLTKRYANGFTAVDGVDFDVRPGKAFGFLGPNG
                     AGKTSTMKMIACASPVTEGTLSVIGMDPRRQAREIKARLGVVPQIDNLDTELTVRENL
                     LMYARYFDIPGDVAKSRASELLEFVQLDERSNDQVEPLSGGMKRRLTIARALINEPDL
                     VILDEPTTGLDPQARHLLWERLYQLKRRGATLIITTHYMDEAEQLCDHLVVMDKAKIV
                     AEGSPRQLIDQHSTKEVLELRLTDSVRGSLNGQLDGLADRIEELPDRLLLYTPNGERT
                     LEQVHERNIPVESALVRRSSLEDVFLRLTGRSLVE"
     gene            5562..6227
                     /locus_tag="VFV59_08030"
     CDS             5562..6227
                     /locus_tag="VFV59_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325818.1"
                     /translation="MPVRVVVQHGPRDRKVAAVALDWPGWSRGAKDPAAALELLETYR
                     DRYRPVAVLAGLEAEFDGAGPLQIVEDHVGVGSTDFWGISFAPSSFEQEPMRDEELDR
                     RLRLLDAAWRYFDSVAARVPNELARGPRGGGRTRDQIVRHVINNERHDLAKRVGVLPT
                     PEEVLSPELVPWHRAAFVAAMREHNAQGRLARGRNWTIALLLRHTAYHALDHAWEMED
                     KAP"
     gene            6224..6892
                     /locus_tag="VFV59_08035"
     CDS             6224..6892
                     /locus_tag="VFV59_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325819.1"
                     /translation="MTTPIRTVVERGPKGKRAVAFAIDWPGWSRGAKSPDEALETLEA
                     YRERYRPVARHAGLDGELDGAPLEIVEDHQGNTSTDFWGISWLPSSFERGPMEPEVLE
                     RRLALLAGAWRFFDEVAARVSPELRRGPRGGGRSRDEIIAHVFGNERVQFSKKVGVAT
                     PPGVMLTPDGLRRHRDQFLAGIRDYNAADRKVGRTWTLQYLVRHATYHVLDHAWELED
                     RDLS"
     gene            complement(6900..>8484)
                     /locus_tag="VFV59_08040"
     CDS             complement(6900..>8484)
                     /locus_tag="VFV59_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEU5325820.1"
                     /translation="ARPMPPSTAPHGMVATPHPAATRAGVEALRAGGTAVDAAVAANA
                     MLAVVYGNACGLGGDAFALVWDPGEGRLHGFNGSGRSPAALTIDRVRGEGHEAMPQRG
                     PLTINVPGAVDAWAQLLERFGRHTLADALAPAARAAEDGYELTRITAGSIAASLPLFD
                     DAARGVFGSAGGPGSTLRQPLLAATLQTIADGGRDAYYLGPIAAEISRAVQAAGGVLS
                     SEDFAAHRGDWVEPISTTYRDHELATIPPNSQGITALIALNVLAGLDWPMGAPLSADR
                     IHAHVEAIKVAWSERDRCVTDPDRDRCHPGDLLTEDHARWLRSRLDVERAHRFMPANP
                     PGGGTVYLCAADADGMLVSLIESNYMGFGSMVMGGSTGIMLQNRGAYFSLDPDHPNAL
                     APSSRTLHTLMPGMLLRDGRAEVALGAMGGDGQPQTMVQVVTGLLDDRLDTQAVVSRP
                     RWVVQTDAPGEPLRRLSIESDGVDDATVAELRRRGHDVELVEPQTPLMGWAQMIRRTA
                     DGSYEGGADPRADSLAAGL"
BASE COUNT         1246 a         2983 c         3067 g         1188 t
ORIGIN      
        1 cgaccagcgc ggcgactcgt cgctggacga cgtgcggcgc acgacgcggc tgggcatggg
       61 cccgtgccag ggcgccttct gcaccccgcg cgccgcggcc gtgctcgacg aacgggcggc
      121 ggccggtcgc ctggcgccgc aggccccagc gcccaacgcg gccatgcgag gattcctcca
      181 ggagcgcttc cggggaccgc ggccgatcgc gtggggcgag cagctcaccg agctgtggct
      241 ctcggccggg atcttccgcg ggctgctggc ggtggacgag ctcccggaca ccagctcccc
      301 gctcgccgag gaccgggtgg atgccgcggg ctgacgtggt cgtcatcggg gctggccttg
      361 cgggcctgtc gtgcgcggcc gagctggcgg aacgcggggc acgcgtcctg ctcgcggcga
      421 agggcatggc gacgacacac tggacccatg gcggcctgga cgtggccggc atcccggggg
      481 ccgcgacccc gcgcgccggc gtggaggcac tgcgctcgaa caccctccat ccgtacgcga
      541 agctcgcgtc cgacgtcggg ccggccgtcg cggcacacct gcggcggacc gacgccgagg
      601 gcctgcccca cgcgggcgac ctcgacgcgt cgctccgtcc catgccgacc gccatcggca
      661 cgctgcggcc ggccgccatc gtgcccgcgg cccaggcgcc ggcgctggcg ccgtggaacg
      721 gcgacgggct gctgctcgtc ggcatcgagc actaccgcga cgcctgggca ccgtgggccg
      781 cccgcaacct ggccagcgcc ttgtggcccg acggcgcccc gcgcgaggtc cggggcatca
      841 cggtcgacct gccgtggctg gatcggctgc acaacctcaa cgccacggtc ctcgcgcgcc
      901 tcttcgacga cccggtgtgg cgcgagcgga cgctcggcgc catcgcgcag gccgtgccgg
      961 cgggcagctg gcgcatcggc ctgccggcgg ccctcgggct cgagcggcac cgcgaggtcc
     1021 acgccgatgc ggaggcggcg ctcgggcatc cgtgcttcga ggtcccgagc ctgccgccat
     1081 cggtgccggg catgcgcctg ttcgaggcgc tgcggcgccg gatcatcggc gcgggcggcc
     1141 ggatccacct gggcttcgag gttgttcagg tcgagcgcga cgagcagcgc gtgcacgcga
     1201 ttcacacgca cgcgggggcc cgcacgctgc gtctgctggg ggatgcgttc gtgctcgcca
     1261 ctggcggcct ggccggtacc ggcttccgcg gccgtcgcga cggcacggtc gaggagcgca
     1321 tcttcgggct gcacgtaacg gcgccaccgc accgggcgtg gttcagcgac gacccgttgc
     1381 agccccaccc gctcgaggcg gcgggcatcg agacggacga cgcgcttcga ccgcccgagc
     1441 tcgagaatgt ccgtgtgatc ggttcggcgc tggccgggat gcactacctc tccgagcgct
     1501 gcggcgacgg cgtggccctg gcatccgcgt accgggtggc ggccgagctc ggcggaaccg
     1561 cgcgggcggc ggcatgacct tccacgacgc ggcgacccgc gacttcgccc ccttcagcac
     1621 cgaccagtgc atcaagtgca cggtgtgcac gacggtctgc ccggtgtcgc gcgtgaccga
     1681 tgcgttcccg gggcccaagt acgtcgggcc gcaggcgcag cgtttccgcg gtcccacggt
     1741 gatggaggcg tcacccgacg tgacggtcga ctggtgctcc ggctgtgggt actgcacccg
     1801 atcctgcccg accggggtgc ggatcgcgga gacgaactcg cgcgcacgcg ccgccatggt
     1861 cgtgcggcgg gggagcggcc tgcgcaatcg gctgatcagc gacaccgacc tcctgatccg
     1921 gctcggcgtg ccggtcgcgc cgctcgccaa cttcgggctc cgaaaccggc tgctgcgctg
     1981 gatcggcgag aagctggttg gcgtgcatcg ccgcggcccg ctcagtccct tcgcgcggcg
     2041 gaccttccga tcctggtgga agagggagcg gcgtccgctg gacctgccgc ccggcgcgga
     2101 cccgcatcgc acggtgatct acttccacgg ctgcgcagtc aacggcttcg agcccgacct
     2161 gggccgccag gccgtcgagg tcctggagcg caacggattc acggtcatct cgcccgacca
     2221 gggctgctgc ggcctgccgt tcatcagcaa cggcctgtac gaccacgcgc gacgcaaggc
     2281 gcgacgcaac gtggagtcct tcgccgagct ggcgcgggcc ggcgtgcgga tcgtgggaac
     2341 ctcgacctcg tgcacctacg ccatgaaggc cgagtaccac gagatgctcg acctggacga
     2401 cgccgatgcg cacgccgtgg cgcgcgccac ctgggacatc tgcgagctgc tggtcgacct
     2461 gcacgaccgc ggcgagctcg acacctcgtt cggaccgatg cacgaggcgg tgccctacca
     2521 cccgccctgc cagctgaagt cgcacggcat cggcaccccg gccatggacc tgttcagcct
     2581 gatccccggc ttccgtgcca ccgattcgga ccacgactgc tgcggcgccg ccgggaccta
     2641 cggcctgaag gtcgagaagt ggcagatcgc gcaggacgtc ggcgcgccgc tgttcgccaa
     2701 ggtccgagcg gccgttgctg acggctcgcg gcgggcggtg tgcgactcgg agacgtgccg
     2761 ctggcagatc gagcagtcca gcggcgtcga cacggtgcac ccgatcaccg tgctggccga
     2821 cgcgtaccgc aggggcgacg aggcggcgcg cgagccgtgg cgccgggtgg tgtgagcggt
     2881 cagctcactt gatgagcttg cgctccatct gccggatggc gatccacatg cccaccgcga
     2941 agaacgccag caggtaggcc aggtcccaca cctgctgcca tcccaccatc ccgagcgaca
     3001 gcccgcggat gagcgagatc gagtggtaca gcggcgtggc ctgcatgaac acctggatga
     3061 accacgggta gatcgtgatc gggaagaagg tgccgctgaa caggaacatc ggctggacga
     3121 tgaggcccat cggcacgtcg aagtcgttca ccgtccgcag gtacgacgtg ccggccaggc
     3181 ccgcggcggc gaaggcggcc gccaccagca gcgcggccgg gatgaccagc aggccccagg
     3241 gcgagaggat caggccggtg accagcatga tcacgaacat ggccaccgcg tacagggcgg
     3301 cgcgcatgag ggcccacatc atctcgccga tggcgatctc cgtgatgccc atcggcgtgg
     3361 ccagcacgcc gtcgtacgtc ttggcgtagt tcagcttgaa gaagatgttg aagatcgtct
     3421 cgaagacggc gccgttcatg gcggccgtgg ccaccagggc gggcgccacg aaggcgacgt
     3481 acgacacctc acggccaccg acgagctcgg gcggcacggt ctgcaccagc ccgccgatgc
     3541 cgagcccgat gcccagcagg tagaagatcg gctcgaagac gcccgagacg atgatgatcc
     3601 actggtggcg gtagaccagg atgttgcgct ccaccagccg gcggccgcgc cagatgcggt
     3661 cccaggcggt ggcccagatg ctcggggcgg agcgccgagg ctccgcggcg atggccgtca
     3721 tcggctcagc tcgccatccg cttgatcagg ttgcgctcgg cgaggaaggc gccgatgccg
     3781 aacatcgcca gcaggtacgc caggtggatc ggcgcggtca gcggatccag ctggtccagg
     3841 atggagcccc ggatcagctc gacgccgtgg aagagcggcg tcgcccaggc gacggcgcga
     3901 atcggctccg gcagctgggt gagcgggaag aaggtgccgc tgaacaggaa cagcgggttg
     3961 atgacgaacc ggaagatggc actgaatccg acgtcgttct tctgcgtcgc cgtgaaggcg
     4021 atgaggcagg cgctgaaggc aaggccgacg agcacggcga ccgggatggc cagcacagcc
     4081 atgggcgtgc gcgggatgcc gaagacggcc agcaccacca ggaagatcgt cgccagcgtc
     4141 cccacacgga acgcgatcca gccgaactcg ccgccgatga ttccgcgcac ggtgaggggc
     4201 gtcgagagca tggcctcgta gttccggccc cacatgatct tgttcatgat cgggtaggtc
     4261 gactcgaacg tccccgtctg catggccatg gcggccatga gccccggccc gagccagtcg
     4321 atgtagtcga cccccccgaa ggcgctcgcc gactggccgc cgatgagcgt cccgatcccg
     4381 atgcccatgg ccgtcaggaa caggaacggc tgcaggaagc cgaagaagag gctccgatgc
     4441 cagaggcgct tgtagacgag ccagttgcgc tgccagacgc gaccggatcc cgcggcgacg
     4501 gtcacgtccg ctactcgacc agcgagcggc cggtgaggcg cagaaagaca tcctccaggc
     4561 tggagcggcg gacgagcgcc gactcgaccg ggatgttccg ctcgtggacc tgctcgaggg
     4621 tccgctcgcc gttcggggtg tagagcagca gccggtccgg cagctcctcg atgcggtcgg
     4681 cgaggccgtc caactggccg ttgaggctgc cgcggacgct atcggtcagg cgcagctcga
     4741 gcacctcctt ggtggagtgc tggtcgatga gctggcgcgg cgacccctcg gccacgatct
     4801 tcgccttgtc catcaccacg aggtggtcgc acagctgctc cgcctcgtcc atgtagtggg
     4861 tggtgatgat cagggtcgcc ccgcggcgct tcagctggta gagccgctcc cagaggaggt
     4921 ggcgcgcctg cgggtcgagg ccggtggtcg gctcgtccag gatgaccagg tccggctcgt
     4981 tgatcagggc gcgggcgatg gtgaggcgac gcttcatccc gcccgacagg ggctcgacct
     5041 ggtcgttcga tcgctcgtcg agctggacga actccagcag ctccgacgcg cggctcttcg
     5101 cgacgtcgcc cgggatgtcg aagtagcgcg cgtacatcag gaggttctcg cggaccgtca
     5161 gctcggtgtc gaggttgtcg atctgcggca cgacgcccag tcgcgccttg atctcgcgag
     5221 cctggcggcg cggatccatg ccgatcacgc tcagcgtccc ctccgtgacc ggcgaggcgc
     5281 aggcgatcat cttcatggtg ctcgtcttgc cggcgccgtt ggggccgagg aagccgaagg
     5341 ccttgccggg tcgcacgtcg aagtccacgc catcgacggc ggtgaagccg ttcgcgtagc
     5401 gcttcgtcag gccgcgggca tggacgagcg agtcgggagt cgtcatgggg ctcgccattc
     5461 tagacccatt cgcccgccga aactcatcgg ccgaatgtcg cgatcggtga cgccgagatt
     5521 catgccaagg gatgtcagca tcgatcccgt accatcggcg catgcccgtt cgcgtcgtgg
     5581 tgcagcatgg gccgcgcgac aggaaggtcg ccgccgttgc cctggactgg ccggggtgga
     5641 gtcgtggcgc gaaggatccg gccgcggcgc tggagctgct cgagacgtac cgcgaccggt
     5701 accggccggt cgctgtgctc gccgggctcg aggccgagtt cgatggcgcc ggtccactgc
     5761 agatcgtgga ggaccacgtc ggcgtcggct ccaccgactt ctggggtatc tccttcgcgc
     5821 cgtcgtcctt cgagcaggag ccgatgcgcg acgaagagct cgatcggcgc ctgcgcctgc
     5881 tcgacgccgc gtggcgctac ttcgacagcg tcgcggcacg cgtccccaac gagctcgcca
     5941 ggggaccgcg tggcggtgga cggactcgag accagatcgt ccgccatgtc atcaacaacg
     6001 agcggcacga cctcgccaaa cgcgtgggcg tcctccccac cccggaggag gtgctctctc
     6061 cggaactggt cccgtggcat cgcgcggcct tcgtggccgc catgcgcgag cacaacgccc
     6121 agggcaggct cgcccgcggc cgaaactgga ccatcgccct gctgctgcgg cacaccgcct
     6181 atcacgcgct cgaccacgcc tgggagatgg aggacaaggc cccgtgacca cgccgatccg
     6241 gaccgtcgtc gaacgcgggc ccaagggaaa gcgcgcggtc gccttcgcca tcgattggcc
     6301 cggatggagc cgcggcgcga agtcccctga cgaggcgctc gagaccctcg aggcctatcg
     6361 cgagcgctac cgaccggtgg cgcgccacgc cggtctggac ggcgagctcg acggtgcccc
     6421 gctcgagatc gtcgaggacc accagggaaa cacctcgacc gacttctggg gcatctcgtg
     6481 gcttccatcg tccttcgagc gcggaccgat ggaacccgag gtactcgagc gtcgcctcgc
     6541 gctgctggcc ggcgcgtggc gcttcttcga cgaggtcgct gcgcgcgtct cgccggagct
     6601 gcgccgcggt ccgcgcggcg gaggccggag ccgcgacgag atcatcgcgc acgtcttcgg
     6661 caacgagcgc gtgcagttct cgaagaaggt cggtgtcgcc acgccgcccg gggtgatgct
     6721 cacccccgac ggcctgcgca ggcaccgcga ccagttcctg gccgggatcc gcgactacaa
     6781 cgccgccgat cggaaggtcg gacgcacgtg gacgctgcag tacctggtcc gccacgcgac
     6841 gtaccacgtg ctcgaccacg cgtgggagct ggaggaccgg gacctcagct agcgcgccgt
     6901 cagagtcccg cggcgaggga gtcggcgcgg ggatcggcgc cgccctcgta cgagccgtcg
     6961 gcggttcggc ggatcatctg tgcccagccc atcagcggcg tttggggctc caccagctcg
     7021 acgtcgtgcc cgcggcgccg cagctcggcg accgtggcgt catccacgcc gtcggactcg
     7081 atcgagagac gccgcaacgg ctcgccgggc gcatcggtct ggacgaccca gcggggccgg
     7141 ctgacgaccg cctgcgtgtc caggcggtcg tccaggaggc cggtgacgac ctggaccatg
     7201 gtctgcggct gcccgtcccc gcccatggcc cccagggcca cctcggcccg gccgtcgcgc
     7261 agcagcatgc ccggcatcag cgtatggagc gtccggctgg acggcgccag cgcgttgggg
     7321 tggtcggggt ccagcgagaa gtaggcgccc cggttctgga gcatgatccc ggtggaaccg
     7381 cccatcacca tcgagccgaa gcccatgtag ttcgactcga tcaggctcac cagcatccca
     7441 tcggcatcgg cggcgcagag gtagaccgtg ccgccacccg gtggattcgc cggcatgaac
     7501 cgatgcgcgc gctcgacgtc cagcctcgac cgcagccagc gcgcgtggtc ctccgtcagc
     7561 aggtcgcccg gatggcagcg gtcgcggtcg ggatcggtga cgcatcggtc gcgctccgac
     7621 cacgcgacct tgatcgcctc gacgtgggcg tggatccggt ccgcgctcag cggcgctccc
     7681 atgggccagt cgaggccggc cagcacgttc agcgcgatca gtgccgtgat gccctgcgaa
     7741 ttcggcggga tggtggccag ctcgtggtcc cggtaggtcg tggagatggg ctccacccag
     7801 tcgccacggt gcgcggcgaa gtcctcgctg ctcagcacgc caccggccgc ctggacggcg
     7861 cggctgatct ccgccgcgat cggcccgagg tagtacgcgt cccgtccgcc gtccgcgatg
     7921 gtttgcagcg tggccgccag gagcggctgc cgcagcgtgg agccggggcc gccggcggag
     7981 ccgaagacgc cccgggcggc gtcgtcgaag agggggagcg aggccgcgat cgagccggcg
     8041 gtgatgcgcg tcagctcgta gccgtcctcg gcggcacggg ccgcgggcgc cagggcatcg
     8101 gccagcgtgt gacgaccgaa gcgctcgagc agctgcgccc aggcgtcgac ggctccgggc
     8161 acgttgatgg tcagcggtcc ccgctgcggc atggcctcgt gcccctcgcc gcggacgcgg
     8221 tcgatggtca gcgcggcggg ggatcgaccg ctgccgttga agccgtggag gcggccctcg
     8281 ccggggtccc agaccagggc gaaggcgtca ccgcccaggc cgcacgcgtt gccgtacacg
     8341 acggcgagca tcgcgttcgc ggcgaccgcg gcgtccaccg ccgtgccacc ggcccgcagc
     8401 gcctcgaccc cggcccgcgt cgccgcggga tgcggggtgg cgaccatgcc atgcggtgcg
     8461 gtggacggcg gcatcggtct ggcc
//
LOCUS       DASTYP010000177        10436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_780448, whole genome shotgun sequence.
ACCESSION   DASTYP010000177 DASTYP010000000
VERSION     DASTYP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780448"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /locus_tag="VFV59_08045"
     CDS             <1..1771
                     /locus_tag="VFV59_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5325821.1"
                     /translation="RARALVLLIVATVVATTIGARLVWWQVFEQPWLAQMALHQLAQH
                     EELPAERGEITDRNGDLLATSVELQSVFATPPTVEDPGLASTILASVLDMPVEEIRSR
                     LESDNPWVWLRRRVSPEVSERIRSLDLRGIGMLPETKRVYPVEGVAGGTTIAAQVIGF
                     VDVEGRGQYGIEAGENALLAGSPGMVTAQEDVIGRRIADSATLLTDPVDGADLRLTLD
                     AGLQHLLEQAIWDAMQKNSAAGAMGVIMDAESGAILGMASFPSYDANQFATTDGELFA
                     NPVISRQYEPGSVMKAFTIAAALDAGAVTTDDTFDDNNDLVVGGIHIHNADRAKFPYG
                     HGPITPADVLALSNNVGAAKIGLALGRTELYEAFRRFGFGSPSGIELSGEAAGVVWDP
                     NGPNASGDLTAAQNAFGQGLSVTALQLAAGYSAFANGGMLVTPHVVEGYTDANGAYHE
                     VQPPAGERIMRPETAQTMVDLLVNSVDEGIANRASVPGYTVGGKTGTAQIAAPVQTTD
                     ANGETVEHWQYIPGWVDSSFVGIMPVGDTKLTVVITINRPAGAGGPAMPMRPEWVFES
                     LMPQVLAYLAIPPDRPLDPVAQP"
     gene            1810..3204
                     /gene="murF"
                     /locus_tag="VFV59_08050"
     CDS             1810..3204
                     /gene="murF"
                     /locus_tag="VFV59_08050"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5325822.1"
                     /translation="MPPTIQLDDLLAAVGGRLQAPTSVTEIAGASVDSRRIVPGSCYV
                     ALRGERVDGHAFVTDALRAGAAAALVDRPVELPPDVDAALVTVDDPLVALQELAAWWR
                     DRHAVRVVGITGSTGKTIAKEIVADVLSRTLTTLRNEGNLNSETGLPMTLLRLQPQHE
                     VAVLEMSMYTTGEIARLAEIARPEVGVVLAVHPTHLQRAGSIERIAQAKSELPAALPA
                     DGLAVLNADDHRVRAMRDVTAARVRTYGFGAEAEVRAVDVVSHGLAGTEFTLDAPWAR
                     TRLRSGSPGRHLVPHALAAAAIAEHFAVPLPEVAAALEAGSHAPHRMHVIHAPSGVTL
                     VDDTYNASPISVRAALDFLAETPVGGSGRRIGVLGDMLELGPDEERLHREIGAHAGRV
                     LDVLVAVGERGRWIAEAARGGTARVVEADDADDAAEVVARDVVPRSGDLVLLKASRGI
                     ELDRTVERLLETDA"
     gene            3201..4187
                     /gene="mraY"
                     /locus_tag="VFV59_08055"
     CDS             3201..4187
                     /gene="mraY"
                     /locus_tag="VFV59_08055"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5325823.1"
                     /translation="MSLVQVSVALLLAFVGMVLLAPIYVSLLQRLGYGKQIRAEGPAA
                     HFAKAGTPTMGGMLMVGVVLFLAMALRIEDAGTLTPMLTLVGVGILGAIDDFVNVRTG
                     VGMRGRWKLVWQTAVAILAAFYIWNHFDLTGLNVPLLGQFEIPAVLLIGFIAFVIVGV
                     SNAVNLTDGLDGLAGGVLIFCFVAYLLVSLVEVPGIKIAQPNLAIFCALVIGALMGFL
                     WFNVHPAQIIMGDSGALSLGATLAVVATVNGQLPLLAVIAIVPFAVIMSVVLQVLSYR
                     LRGRRIFRMTPLHHHFELLGWAEEKITVRFWIVSALAGLLGFGLFLASVNEL"
     gene            4199..5608
                     /gene="murD"
                     /locus_tag="VFV59_08060"
     CDS             4199..5608
                     /gene="murD"
                     /locus_tag="VFV59_08060"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU5325824.1"
                     /translation="MTAPIAPVRSVQDLRGRRSVVLGLARSGVAAARFLADAGAVVAV
                     YDRRPATELSEAVAALGGRPVRLSLDVDPPEATALLENADLLVTSPSVSARYPTTDPW
                     LREALRAAEARGAEVISEVGLFLRLTAARVLGVTGTKGKTTTSSLVAAMLEAGGVPTA
                     LGGNFGTPLIERATELTAEDWVVLELSELQLPTIDRGVDVALYTNIGEDHLDRHGTVE
                     AYRAVKARLAELTAPAGRVVLNRDDAGCRELGERLPAAAVAWYGIERPGVGPTEAWTD
                     DAGWLLVDGQRLLPASEVPLPGLHMRADVLGAALAATLVGVAPEAITEAIREFRGVAH
                     RLELITDRGGIRYVNDSQATIPVATMAALDAFEAPIVLIAGGKDKGLEYAELADRIAE
                     RCRAVVLIGETADELERQIDGRVPVVHVRTMDEAVAQAASRADPGDVVLLSPAAASFD
                     MFVDYAARGDAFRRAVLRA"
     gene            5605..6879
                     /gene="ftsW"
                     /locus_tag="VFV59_08065"
     CDS             5605..6879
                     /gene="ftsW"
                     /locus_tag="VFV59_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEU5325825.1"
                     /translation="MTTALSATAATASRRTAARGGVRRERHEPAYALLLAVLALTAIG
                     VVMVYSASSVRSYFSTSDPASQGLEQLVWAGIGLTALVVAMRLDFRHLRYLAIPFFVI
                     TLGLLVAVLIPGIGAEINGSRRWIIIPGVGTLQPAEFAKLAVVLYLAHWLDRRGKEAR
                     SFWNGLVPFGLLVAPGFLLIAMEPDLGTAGVYAIIAISIFFMAGANLLYLGAIGSAVV
                     AAAWLMVSATSYQLERVQTFLDPFRDPLGSGYNTVQALMALGLGGITGLGLGESRQKF
                     LYLPAPSTDFIFAIIGEEWGLVGTLTVVSLFVVIAYQGYRIAITAPDTFSGLVACGVT
                     TWLVVQAFVNMMVVAALLPVTGIPLPFISAGGSALTINLAAVGILLSISRETTQTGSL
                     RDAVFGVGRRDRRAHLPRAGRRPGPARRTTRR"
     gene            6785..7894
                     /locus_tag="VFV59_08070"
     CDS             6785..7894
                     /locus_tag="VFV59_08070"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU5325826.1"
                     /translation="MRYSVSGGGTGGHIYPALAVAQALRDARPDVELDYVGGVRGFER
                     RLVASHPLAAQLRYHELVVRSLRSAGLSVHTALDPVRLAASVPQAWWLLGRLRPAAIF
                     TTGGYLALPLVLAARARGIPTLVWEGNVLPGRATRAIGRLATRVAVSFPPTLDAFPGN
                     SFVSGTPIRSFDGIDRAAARHALGLDPDDRLLLVFGGSQAVARMNAAVTDALPRLLPD
                     WHVLHLAGEAGMADAGAARRRLPDDLRPRYTAEPFLTDRMADALVAADLVLGRAGSST
                     CAEVAAVGVASILVPYPYAGAHQQYNARYLADEGAAIMVADEDLTAARLVAEVGALRS
                     DDARRAMADAARRLGRPDAAQALADAVIELAEGRS"
     gene            7891..8856
                     /gene="murB"
                     /locus_tag="VFV59_08075"
     CDS             7891..8856
                     /gene="murB"
                     /locus_tag="VFV59_08075"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5325827.1"
                     /translation="MSGAVISASALERLDELSSARGLSLTQDAPMAPLTTLRVGGPAD
                     RLAAPESVDELVALLEVAAEAQVPVFLLGKGSDIVVADAGIRGLVIRTRADAVSIDGR
                     RLRAEAGTSMTALAKRCARAGLAGFDWAISIPGSFGGAVWANAGAHGGEMAQVLREVE
                     AWDPRTRRRVLLAPSDCAFSYRESRFKHSGEIVLGATIELGRGEPTAIAARIAEHQAQ
                     RKATQPLADQNAGSVFRNPPGDHAGRLIDAAGLKGHRIGSAQVSTLHANFIVTDRGTA
                     RAADVRAVGDHARRTVAERFGIHLQYEIEFVGDWPAGALDQARDA"
     gene            8853..9965
                     /locus_tag="VFV59_08080"
     CDS             8853..9965
                     /locus_tag="VFV59_08080"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019007716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEU5325828.1"
                     /translation="MNQRLRVGVVAGGRSAEHEVSIASADAVLRAIDRDRYEPYLVYI
                     DREGRWRLPDGPAPRLGDGALLAELVGAATPAEEVERLRAHGSHDLATVADARALAAG
                     TAVRSLAETIDVAFLVVHGPYGEDGTLQGFLELAGIPYVGAGVLASAVAMDKVVFKDL
                     MRGHGLPVVDAVSFRRHAWEQEPEHVVREVAETIGERTVVKPARLGSSVGMSIVHDLD
                     ELPAALDEAFRYDSKVIVEAYVANARELECGVLGTNEPIVFEPGEVHSTHEFYDYEAK
                     YVDGLAVVEPRADVDPELAARLRELALAAYRAVDGAGLARIDFLVDGDEPYISEMNTL
                     PGFTRTSMYPKQAELAGIGFPELIDRLIALAMERER"
     gene            9962..>10436
                     /locus_tag="VFV59_08085"
     CDS             9962..>10436
                     /locus_tag="VFV59_08085"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU5325829.1"
                     /translation="MKLKPQPKPKPMRAGRRPSRGAARTSVRGGGRGMAKRPGVPLRQ
                     RIRRRLPSPHRVLAVLAAGASAAVLVALLNGPWLRVADVAWAGERYTDPRSIGELLDA
                     QRGASLLGVNTDALQRRLERIPSIADATVRASITGRLEATVIEREAAFVWATNFGR"
BASE COUNT         1355 a         3767 c         3822 g         1492 t
ORIGIN      
        1 ccgtgcccgc gcactggtcc ttctgatcgt cgccaccgtc gtggcgacga cgatcggcgc
       61 tcgcctggtg tggtggcagg tcttcgagca gccctggctg gcccagatgg ccctccacca
      121 gctcgcccag cacgaggagc tgccggccga gcgcggtgag atcacggacc gcaacggcga
      181 tctgctcgcc acctccgtcg agctgcagtc ggtcttcgcg acgccgccga cggtcgagga
      241 cccgggactg gcgtcgacca tcctcgcatc ggtcctggac atgccggtcg aggagatccg
      301 gtccaggctc gagagcgaca acccctgggt ctggctccgg cgtcgcgtct cgcccgaggt
      361 ctcggagcgc attcgcagcc tcgaccttcg gggcatcggc atgctgccgg agacgaagcg
      421 cgtgtacccg gtggagggcg tggccggcgg gacgaccatc gccgcccagg tcatcgggtt
      481 cgtggacgtc gagggccgcg gccagtacgg gatcgaggcc ggcgagaacg ccctactggc
      541 tggttcaccg ggcatggtga ccgcgcagga ggacgtgatc gggcgccgga tcgccgactc
      601 ggcgacgctg ctgacggacc cggtcgacgg tgccgacctg cgcctcaccc tggacgccgg
      661 cctgcagcac ctgctcgagc aggcaatctg ggacgccatg cagaagaaca gcgcggccgg
      721 ggccatgggc gtgatcatgg atgccgagtc gggcgcgatc ctgggcatgg ccagcttccc
      781 gtcgtacgac gccaaccagt tcgccacgac cgatggcgag ctgttcgcca acccggtcat
      841 ctcgcggcag tacgagcccg gatcggtgat gaaggccttc accatcgcgg cggcactcga
      901 cgcgggcgcg gtcaccacgg acgacacgtt cgacgacaac aacgacctgg tcgtcggcgg
      961 gatccacatc cacaacgccg accgcgcgaa gttcccgtac ggccacgggc cgatcacgcc
     1021 ggccgacgtc ctggccctgt cgaacaacgt gggcgccgcg aagatcggcc tggccctggg
     1081 gcgcacggag ctgtacgagg cgttccggcg cttcggcttc ggctcgccga gcggcatcga
     1141 gctgtccggc gaggcagcgg gcgtggtgtg ggacccgaac ggccccaatg cttccggcga
     1201 cctcaccgcg gcgcagaacg cgttcggcca gggcctcagc gtgaccgccc tgcagctggc
     1261 ggccggctac tcggccttcg ccaacggcgg gatgctcgtg accccgcacg tggtcgaggg
     1321 ctacaccgac gcgaacggcg cgtaccacga ggtgcagccg ccggccgggg aacggatcat
     1381 gcggccggag acggcgcaga cgatggtcga cctgctcgtg aactcggtgg acgagggcat
     1441 tgccaaccgc gcgtcggtgc ccggatacac cgtgggcggc aagaccggca ccgcgcagat
     1501 cgccgccccc gttcagacga ccgacgccaa cggcgagacg gtcgagcact ggcagtacat
     1561 cccgggctgg gtcgactcca gcttcgtcgg gatcatgccg gtcggcgaca ccaagctgac
     1621 cgtggtgatc accatcaacc gcccggcggg ggccggcggt ccggccatgc cgatgcgtcc
     1681 ggaatgggtg ttcgagtccc tgatgccgca ggtcctggcc tacctggcca tcccgccgga
     1741 ccggccgctc gaccccgtgg cgcagccatg aggctggtag actcgcccgg attccgcagc
     1801 gtgcccggtg tgccccccac gatccagctc gacgacctcc tggccgccgt cggcggccgg
     1861 ctccaggccc ccacctccgt caccgagatc gccggggcct ccgtcgactc ccgccggatc
     1921 gttcccggaa gctgctacgt cgccctccgc ggtgagcgcg tggacggaca cgccttcgtg
     1981 accgatgcgc tgcgcgccgg tgccgcggcc gcgctggtgg accgccccgt cgagctgccg
     2041 cccgacgtcg acgcggccct ggtcaccgtc gacgacccac tggtcgcgct ccaggagctc
     2101 gcggcatggt ggcgcgaccg gcacgccgtg cgcgtggtgg gcatcaccgg ttcgaccggc
     2161 aagaccatcg ccaaggagat cgtcgccgac gtgctgtcgc gcacgctgac cacgctgcgc
     2221 aacgagggga atctcaactc cgagaccggc ctgccgatga cgctgctgcg cctccagccg
     2281 cagcacgagg tggcggtgct cgagatgagc atgtacacca ccggtgagat cgcccggctg
     2341 gccgagatcg cccggcccga ggtgggcgtg gtgctggcgg tgcacccgac gcacctgcag
     2401 cgcgcgggga gcatcgagcg catcgcgcag gccaagagcg agctgccggc cgcgctcccc
     2461 gccgacgggc tggcggtgct gaacgccgac gaccaccgcg tgcgcgccat gcgcgacgtg
     2521 acggcggcgc gcgtccggac gtacggcttc ggcgcggagg ccgaggtgcg ggccgtcgac
     2581 gtggtgagcc acgggttggc ggggacggag ttcacgctcg acgcgccgtg ggctcgaacc
     2641 cggctgcgga gcggctcacc gggccgccac ctcgtcccgc acgccctggc cgccgcggcc
     2701 atcgcggagc acttcgccgt gccgctgccg gaggtggccg cggccctcga ggccggaagc
     2761 cacgcgccgc accgcatgca cgtgatccat gcgccgtccg gcgtcacgct cgtcgacgac
     2821 acctacaacg cctcgcccat cagcgtgcgc gcggcgctcg acttcctggc cgagacgccg
     2881 gtcggaggat ccggccggcg catcggtgtc ctgggcgaca tgctggagct cgggcccgac
     2941 gaggagcggc tgcaccgcga gatcggcgcg cacgccggac gggtgctgga cgtgctggtg
     3001 gcggtcgggg agcgcggccg ctggatcgcg gaggccgcgc gcggcggcac ggcacgcgtc
     3061 gtggaggcgg acgacgccga cgacgcggcc gaggtcgtgg cacgggacgt ggtgccgcgc
     3121 agcggcgacc tggtgctgct caaggcgtcc cgcgggatcg agctggaccg gacggtcgag
     3181 cggctgctgg agacggacgc atgagcctgg tgcaggtctc cgtcgcgctg ctcctcgcct
     3241 tcgtcggcat ggtgctgctg gctcccatct acgtctcgct gctgcagcgc ctggggtacg
     3301 ggaagcagat ccgcgccgag ggtccggcgg cgcacttcgc caaggcgggg acgccgacga
     3361 tgggcgggat gttgatggtc ggcgtggtgc tcttcctggc catggccctg cgcatcgagg
     3421 acgccggcac cctgacgccg atgctgacgc tcgtcggcgt ggggatcctg ggcgcgatcg
     3481 acgacttcgt gaacgtccgg accggcgtcg gcatgcgcgg ccgctggaag ctggtgtggc
     3541 agaccgccgt cgccatcctg gccgcgttct acatctggaa ccacttcgac ctgaccggcc
     3601 tgaacgtgcc gctgctgggg cagttcgaga tcccggccgt gctgctcatc ggcttcatcg
     3661 ccttcgtgat cgtcggggtc agcaacgcgg tgaacctgac cgacggcctc gacggcctcg
     3721 ctggcggcgt gctgatcttc tgcttcgtcg cctacctgct ggtcagcctg gtcgaggtgc
     3781 cgggcatcaa gatcgcgcag ccgaacctcg cgatcttctg cgccctggtc atcggcgcgc
     3841 tgatgggctt cctgtggttc aacgtccacc ccgcgcagat catcatgggc gactccgggg
     3901 cgctgagcct gggggccacg ctcgccgtgg tggccaccgt caacggccag ctgccgctgc
     3961 tggcggtcat cgccatcgtg ccgttcgccg tgatcatgag cgtcgtcctg caggtgctgt
     4021 cgtacaggct gcggggccgg cggatcttcc gcatgacgcc gctgcaccac cacttcgagc
     4081 tgctcggctg ggccgaggag aagatcaccg tccgcttctg gatcgtctcg gcgctggccg
     4141 ggctgctggg cttcgggctc ttcctggcca gcgtgaacga gctctaggtg gaccgatgat
     4201 gacggcgccg atcgcccccg tccgcagcgt ccaggacctg cgcggccgcc gcagcgtggt
     4261 gctcggcctg gcccgctcgg gcgtggccgc cgctcgcttc ctggccgatg ccggcgccgt
     4321 ggtggccgtg tacgaccgcc gccctgccac cgagctctcg gaagcggtcg ccgcgctcgg
     4381 cggccgcccc gtgcgcctct ccctcgacgt cgatccgccc gaggcaacgg ccctcctcga
     4441 gaacgccgac ctgctggtca cgagcccctc ggtctcggcc cgctacccca cgactgaccc
     4501 gtggctccgc gaggcgctgc gcgccgcgga agcccgcggc gcggaggtca tcagcgaggt
     4561 gggcctcttc ctgcgcctga ccgcggcccg cgtcctgggc gtcaccggga ccaagggcaa
     4621 gacgacgacc tcctcgctgg tggcggccat gctcgaggcc ggtggcgtcc ccacggcgct
     4681 gggtgggaac ttcgggacgc cgctgatcga gcgagccacc gagctgaccg ccgaagactg
     4741 ggtggtgctc gagctctcgg agctccagct gccgacgatc gaccgcggcg tcgacgtggc
     4801 gctgtacacg aacatcgggg aggaccacct cgatcggcac ggcaccgtcg aggcgtaccg
     4861 ggcggtgaag gcccggctgg cggagctcac ggcgccggcg ggacgcgtgg tcctcaaccg
     4921 cgatgacgcc gggtgccggg agctcggcga gcggctgcct gccgccgccg tcgcgtggta
     4981 cggcatcgag cgcccgggcg tcggaccgac cgaggcctgg acggacgacg ccggctggct
     5041 gctggtggac ggccagcggc tgctgcccgc gtcggaggta ccgctgcccg ggctgcacat
     5101 gcgcgccgac gtgctcgggg cggccctggc cgcgacgctg gtcggtgtcg caccagaggc
     5161 gatcaccgag gcgatccgcg agttccgcgg cgtcgcgcac cggctcgagc tcatcaccga
     5221 tcggggcggc atccgctacg tcaacgactc gcaggccacc atccccgtcg ccaccatggc
     5281 ggcgctggac gcgttcgagg cgccgatcgt cctcatcgcc ggaggcaagg acaaggggct
     5341 cgagtacgcg gagctggccg accggatcgc ggagcgctgc cgggcggtgg tcctgatcgg
     5401 ggagacggcc gacgagctgg agcggcagat cgacggccgc gttcccgtcg tccatgtgcg
     5461 caccatggac gaggcggtgg cgcaggcggc gtcgcgcgcg gacccgggcg acgtcgtcct
     5521 cctctcgccg gcggcggcga gcttcgacat gttcgtcgac tacgcggctc gcggcgacgc
     5581 gttccgccga gcggtgctgc gcgcatgacg accgccctct cggccaccgc cgccaccgcg
     5641 tcccgacgca ccgccgcgcg cggcggcgtt cgtcgcgagc gtcacgagcc ggcgtacgcg
     5701 ctcctgctgg cggtgctggc gttgaccgcc atcggcgtgg tgatggtgta ctcggcgtcg
     5761 agcgtccgct cgtacttcag cacctcggat ccggccagcc agggcctcga gcagctggtg
     5821 tgggccggca tcggcctgac ggcgctggtc gtcgccatgc ggctcgactt ccgccacctg
     5881 cgctacctgg ccatcccgtt cttcgtcatc accctggggc tgctggtggc cgtgctgatc
     5941 ccgggcatcg gtgccgagat caacgggtcg cggcgctgga tcatcatccc cggcgtcggg
     6001 acgctgcagc cggccgagtt cgcgaagctg gccgtggtgc tctacctggc ccactggctc
     6061 gaccgccgcg gcaaggaggc ccgcagcttc tggaacggcc tggtcccgtt cgggctgctg
     6121 gtggcgcccg gcttcctgct gatcgccatg gagcccgacc tcgggacggc gggcgtctac
     6181 gccatcatcg ccatcagcat cttcttcatg gccggggcga acctgctgta cctgggcgcc
     6241 atcggctcgg cggtcgtggc ggcggcctgg ctgatggtca gcgccaccag ctaccagctg
     6301 gagcgcgtgc agacgttcct ggacccgttc agggacccgc tcgggtcggg ctacaacacg
     6361 gtgcaggcgc tcatggccct cgggctgggc gggatcaccg ggctggggct gggtgagagc
     6421 cggcagaagt tcctgtacct gccggcgccg tcaaccgact tcatcttcgc gatcatcggc
     6481 gaggagtggg ggctggtcgg cacgctcacc gtggtatcgc tgttcgtggt gatcgcctac
     6541 cagggctacc ggatcgccat cacggcgccc gacacgttct ccggcctggt cgcctgcggc
     6601 gtgacgacgt ggctggtggt gcaggccttc gtcaacatga tggtggtcgc cgccctgctg
     6661 ccggtgaccg gcatcccgct tccgttcatc agcgccggtg gatcggcgct gaccatcaac
     6721 ctcgcggcgg tcggaatcct gctgtccatc agccgcgaga ccacccagac aggatccctg
     6781 cgcgatgcgg tattcggtgt cgggcggcgg gaccggcggg cacatctacc ccgcgctggc
     6841 cgtcgcccag gccctgcgcg acgcacgacc cgacgttgag ctcgactacg tcggcggcgt
     6901 gcgcggcttc gagcgacggc tcgtggccag ccacccgctg gccgcccagc tgcgctacca
     6961 cgagctggtc gtgcgctcac tgcgctccgc cggcctgtcg gtgcacacgg cgctcgatcc
     7021 ggtccgcctg gccgcgtcgg tcccgcaggc gtggtggctg ctgggtcgtc tccggccggc
     7081 cgccatcttc acgacgggcg gctacctggc cctgccgctc gtcctggcgg ctcgcgcccg
     7141 gggtatcccg accctggtct gggagggcaa cgtgctgccc ggccgcgcga cccgcgccat
     7201 cggccgcctg gcgacgcggg tggccgtcag cttcccgccc accctcgacg ccttcccggg
     7261 caacagcttc gtctccggca cgcccatccg ttcgttcgat ggcatcgacc gcgcggcggc
     7321 acgccacgcc ctgggcctcg acccggacga ccggctgctc ctcgtgttcg gcgggtcgca
     7381 ggcggtggcg cgtatgaacg ccgcggtgac cgatgcgctg ccgcgcctcc tccccgactg
     7441 gcacgtcctg cacctggccg gcgaggccgg catggcggat gcgggcgccg cgcgacgccg
     7501 cctgcccgac gacctgcgtc cgcgctacac cgccgagccc ttcctgaccg atcgcatggc
     7561 cgatgcgctg gtcgcggccg acctggtcct cggtcgcgcc ggctcgtcca cctgcgcgga
     7621 ggtcgcggct gtcggggtgg cctcgatcct ggtgccgtac ccgtacgccg gcgcccacca
     7681 gcagtacaac gcgcgctacc tggccgacga gggcgccgca atcatggtcg cggacgagga
     7741 cctgaccgcc gcccggctgg ttgctgaggt cggcgcgctg cgctccgacg acgcgcggcg
     7801 agcgatggcg gatgccgccc gacgcctcgg gcggccggac gcggcgcagg cgctggccga
     7861 cgcggtgatc gagctcgcgg agggtcgctc gtgagcggag ccgtgatttc cgcgtcagca
     7921 ctcgagcggc tggacgagct gtccagcgcc cgcgggctgt cgctgaccca ggacgcgccg
     7981 atggcgccac tgaccacgct tcgcgtcggc gggccggcgg accggctggc cgcccccgag
     8041 tcggtcgacg agctcgtggc gctgctcgag gtcgccgccg aggcgcaggt gccggtcttc
     8101 ctgctcggca agggaagtga catcgtcgtg gccgatgccg gcattcgcgg gctcgtcatc
     8161 cggacccgcg ccgacgcggt gagcatcgac ggccgccggc tgcgcgccga ggcgggcacg
     8221 tcgatgaccg cgctggccaa gcgctgcgcc cgcgcgggac tggccggctt cgactgggcc
     8281 atcagcattc ccggctcgtt cggcggcgcg gtgtgggcca acgcgggtgc gcacggcggg
     8341 gagatggcgc aggtgctccg cgaggtcgag gcctgggacc cgaggaccag acgacgcgtg
     8401 ctcctggcgc cgtccgactg cgcgttctcc taccgcgagt cgcgcttcaa gcacagcggc
     8461 gagatcgtgc tgggggcgac catcgagctg gggcggggcg agcccacggc gatcgccgca
     8521 cgcatcgccg agcaccaggc gcagcgcaag gcgacgcagc cgctggccga ccagaacgcg
     8581 ggcagcgtct tccgcaaccc acccggcgac cacgccggcc gtctgatcga cgcggccggg
     8641 ctcaagggcc atcgcatcgg ctcggcgcag gtctcgacgc tgcacgcgaa cttcatcgtc
     8701 accgaccggg gaaccgcccg cgccgcggac gtgcgggccg tcggtgacca cgcccgccgc
     8761 accgtcgcgg agcgcttcgg gatccatctc cagtacgaga tcgagttcgt cggcgactgg
     8821 cccgccggcg cgctggacca ggcgagggac gcgtgaacca gcgattgcgc gtcggcgtcg
     8881 tcgccggcgg acgctccgcc gagcacgagg tcagcatcgc gtcggccgat gcggtgctgc
     8941 gggccatcga ccgcgaccgg tacgagccgt acctggtcta catcgatcgc gagggccgct
     9001 ggcggctgcc ggacggcccg gcgccgcgcc tcggcgacgg ggcgctcctg gcggaactgg
     9061 tcggcgccgc cacgccggcc gaggaggtgg agcgcctccg cgcgcacggc tcgcacgacc
     9121 tcgccaccgt cgcggacgcg cgggccctcg cggcgggcac cgccgtgcgc tcgctggccg
     9181 agaccatcga cgtggcgttc ctggtggtgc acgggccgta cggggaggac gggacgctcc
     9241 agggcttcct cgagctcgcc ggcatcccgt acgtcggcgc gggcgtgctg gccagcgccg
     9301 tggccatgga caaggtcgtc ttcaaggacc tgatgcgcgg ccacgggctg cccgtcgtgg
     9361 acgcggtctc cttccggcgc cacgcctggg agcaggagcc cgagcatgtc gtgcgcgagg
     9421 tggccgagac gatcggcgag cgaaccgtcg tcaagcccgc gcggctgggc agcagcgtgg
     9481 gcatgtcgat cgtccacgac ctggacgagc tgccggcggc actggacgag gccttccgct
     9541 acgacagcaa ggtcatcgtg gaggcctacg tcgccaatgc ccgtgagctc gagtgcggag
     9601 tgctgggcac caacgagccg atcgtcttcg agccgggcga ggtgcactcg acgcacgagt
     9661 tctacgacta cgaggccaag tacgtcgacg gcctggcggt cgtcgagccc cgggcggacg
     9721 tggatcccga gctcgcggcg cgcctgaggg agctggcgct ggccgcgtac cgggcggtcg
     9781 acggcgcggg actggcgcgc atcgacttcc tggtggacgg cgacgagccg tacatcagcg
     9841 agatgaacac cctgcccggc ttcacgcgga ccagcatgta ccccaagcag gcggagctgg
     9901 ccggcatcgg cttcccggag ctgatcgatc ggctcatcgc gctggccatg gagcgcgagc
     9961 gatgaagctg aagccgcagc ccaagccgaa gccgatgcgc gccggccgcc ggccgtcgcg
    10021 gggtgccgcc cggacgtccg tccgaggcgg cgggcggggc atggcgaagc ggcccggggt
    10081 gccgctgcgg cagcggatcc ggcgcaggct gccctcgccg caccgggtcc tcgcggtcct
    10141 cgccgcgggg gcgagcgccg ccgtgctcgt ggcgctgctc aacggcccat ggctgcgcgt
    10201 cgccgacgtc gcatgggcgg gggagcggta cacggatcct cgcagcatcg gcgagctgct
    10261 ggacgcgcag cgcggcgcga gcctgctggg cgtaaacacc gacgcgctgc agcggcgact
    10321 ggagcgcatc ccgtccatcg ccgatgcgac ggtgcgcgcc agcatcaccg ggcggctcga
    10381 ggccaccgtc atcgagcgcg aggcggcgtt cgtgtgggcc acgaacttcg gccggt
//
LOCUS       DASTYP010000178         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_233468, whole genome shotgun sequence.
ACCESSION   DASTYP010000178 DASTYP010000000
VERSION     DASTYP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233468"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFV59_08090"
     CDS             <1..975
                     /locus_tag="VFV59_08090"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU5325830.1"
                     /translation="VFRDGDEVYVQSRDLKPLNRYFPELEQPLRALGGADARFVLDGE
                     VVIAHPDGGLDFDALLLRIHPAASRVKMLSEQSPASFVAFDCLADADAVLREAPFADR
                     RAALERVVADPPASVHLTPSTTDPAVARHWFDVFEGAGLDGVIAKRASDPYAPGKRTM
                     AKIKHQRTADCVVAGFRWHKNGPGTLVGSLLLGLWNDDGHLQHVGVTSSFTMAKRAEL
                     VEFLAPYRENVQDHPWREWALAEAAAAEEGRRLPGATSRWNRGKDLSWEPVRPELVCE
                     VAFDHLQGDRFRHAATFQRWRPDRSPADCRYDQLEETPPALLADLFGR"
     gene            1052..>2352
                     /locus_tag="VFV59_08095"
     CDS             1052..>2352
                     /locus_tag="VFV59_08095"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5325831.1"
                     /translation="MPVAADPNAAVNQLADRFWDGVLERDPLYATILGDERFNDRWPD
                     LGPDGRAADEAAYRATLADAEAIDPDGLDPEQVITRDLLMLVCRNHLEFLAQKQYQLA
                     LDHMAGPQVWPVQAAQYQPADTDERLAKLLRRYETYPTMIEQYVGTLAEGVADGRTAA
                     AVPVRRALEQIDRLLAIPADQAPAATMAKVADDDRSQVVAAVEEHVYPALRRLRSFLA
                     DEYQPHARQEPGLSATPGGEEAYRLAIRMQTTLDTTAEEVHAFGLADLDAIEAEMDEI
                     AHRLGHADRVALKAALADDPANHTDDPSALVRLAQEQTDRAYAVAPRYFGRLPSANCE
                     VRAVEAYREAESPPAFYMPPSLDGSRQGQYYINTYQPAERQLHKIAAITFHEATPGHH
                     FQIAIEMELKGLPAFRTLGARMAGVAYAEGWGLYCERLADE"
BASE COUNT          337 a          872 c          817 g          326 t
ORIGIN      
        1 gtcttccgtg atggcgacga ggtctacgtc cagtcgcgcg acctcaagcc actgaaccga
       61 tacttcccgg agctggagca gcccctccgt gcgctcggcg gcgccgatgc gcgcttcgtg
      121 ctcgatggcg aggtcgtcat cgcccacccg gatggcggcc tggacttcga cgccctgctg
      181 ctccgcatcc atcccgccgc ctcccgggtg aagatgctct ccgagcagtc gcccgcctcc
      241 ttcgtcgcct tcgactgcct ggccgatgcc gatgccgtcc tgcgtgaggc gcccttcgcc
      301 gatcgccgcg ccgcgcttga gcgggtggtg gcggacccgc ccgcatcggt ccatctgacg
      361 ccgtcgacga cggacccggc ggtggcgcgc cactggttcg acgtcttcga gggcgccggg
      421 ctggacggcg tgatcgccaa gcgcgcatcg gacccgtatg cgcccggcaa gcgcacgatg
      481 gccaagatca agcaccagcg caccgcggac tgcgtggtcg ccggattccg ctggcacaag
      541 aacggcccgg ggacgctggt cggctcgctg ctgctgggcc tgtggaacga cgatgggcac
      601 ctgcagcacg tcggcgtcac ctcgtcgttc accatggcga agcgggcgga gctggtcgaa
      661 ttcctggccc cgtaccgcga gaacgtgcag gaccatccgt ggcgcgagtg ggcgctggcc
      721 gaggccgcgg ccgcggagga gggacgtcgg ctccccggcg ccacgtcgcg ctggaaccgc
      781 ggcaaggacc tgtcgtggga gccggtgcgc cccgagttgg tgtgcgaggt cgccttcgac
      841 cacctgcagg gcgaccggtt ccgccatgcc gcgaccttcc agcgctggcg ccccgatcgg
      901 tccccggccg actgccgcta cgaccagctc gaggagacac cgccggcgct gctggccgac
      961 ctcttcggcc gttaggcggt cgccctcgcc cggtccagcg cggcgctaca ctgcgcgggc
     1021 ctgcccgccg acttccgctg gaggatcacc cgtgcccgtc gctgccgatc ccaacgccgc
     1081 cgtcaaccag ctcgccgacc gattctggga cggcgtcctc gagcgcgatc cgctgtacgc
     1141 cacgattctc ggggacgagc gcttcaacga tcggtggccg gacctcgggc ccgacggccg
     1201 tgccgccgac gaagcggcct accgcgccac cctggccgat gcggaggcga tcgaccccga
     1261 cggcctcgat cccgagcagg tcatcacgcg cgacctgctg atgctcgtgt gtcgcaacca
     1321 cctggagttc ctggcgcaga agcagtacca gctggcgctc gaccacatgg cgggtccgca
     1381 ggtctggccg gtgcaggcgg cccagtacca gccggccgat acggacgagc ggctggcgaa
     1441 gctgctgcgg cgctacgaga cctacccgac gatgatcgag cagtacgtcg gcacgctggc
     1501 ggagggcgtc gcggacggcc gcaccgccgc cgcggtgccg gtgcgtcgcg cgctcgagca
     1561 gatcgaccgg ctgctggcca ttccggcgga ccaggcccca gccgccacca tggccaaggt
     1621 cgcggatgac gaccgctcgc aggtggtggc cgccgtcgag gagcacgtct accccgccct
     1681 gcgccggctg cgatcgttcc tggccgatga gtaccagccg cacgcccgcc aggagccggg
     1741 cctgagcgcc acgccgggtg gcgaggaggc gtatcggctc gccatccgga tgcagacgac
     1801 cctcgacacc acggcggagg aggtgcacgc cttcgggctc gccgacctcg acgccatcga
     1861 ggccgagatg gacgagatcg cgcatcggct ggggcatgcc gaccgggtgg cgctgaaggc
     1921 agccctggcc gacgatccgg ccaaccacac cgacgatccg tcggcgctcg ttcggctggc
     1981 gcaggagcag accgaccgcg cctacgccgt cgcgccgcgg tatttcgggc gcctgcccag
     2041 cgcgaactgc gaggtgcgcg ccgtcgaggc ctaccgcgag gccgagtcgc cgcccgcctt
     2101 ctacatgccg ccctcgctcg acggctcgcg gcaggggcag tactacatca acacgtacca
     2161 gccggccgag cgtcagctcc acaagatcgc ggcgatcacc ttccacgagg cgacgcccgg
     2221 gcaccacttc cagatcgcga tcgagatgga gctgaagggg ctgcccgcgt tccgaaccct
     2281 cggcgcccgg atggccggcg tggcctacgc cgagggctgg gggctgtact gcgagcggct
     2341 cgccgacgag at
//
LOCUS       DASTYP010000179         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_744371, whole genome shotgun sequence.
ACCESSION   DASTYP010000179 DASTYP010000000
VERSION     DASTYP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744371"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VFV59_08100"
     CDS             <1..1176
                     /locus_tag="VFV59_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEU5325832.1"
                     /translation="ERAEEALSLPLIDNAIPEEAVWQCTTCGWCVEGCPVLIEHVDTI
                     VEIRRNAVLEESRFPKELNAAFRNMENAGNPWGQPKNARLDWAKGLDVPVLGQTDTRL
                     RANANGNGDGAAGAPARWRTIHESAPSGDPIGGRVLFWVGCAGAFDDRNRKVVRAMAQ
                     LLAQAGVDFAVLGAQETCTGDPARRAGNEYLFQMLAEENVATLSAAHAEHGISTIVAT
                     CPHCFNTIRNEYPQFGLNGVHVLHHTQLLDRLVAEGRLVPSQHHQAMVAYHDACYLGR
                     YNEVYDEGRRVVASVPGQEVVEMDLHHRKGMCCGAGGARMWMEEREGHRINHRRVEQA
                     LEKSPDQIATACPFCLIMLRDGTIDLDRGDVEVRDVAELLADATGAWNREVATPPEA"
     gene            1490..1975
                     /locus_tag="VFV59_08105"
     CDS             1490..1975
                     /locus_tag="VFV59_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU5325833.1"
                     /translation="MAVRNLSQPGRRPVLDDLDKAIIKALQLDGRRPYAQIGRQLKVP
                     EATVRQRAERLITRGVVQIVGVTDPLSMGFQQPALIGLKVDAGRLDSIAEQIAALEEV
                     TYVVITAGRFDMVCEVVCEDNDHLLRVLTEQFARIEGIRSTETLVELRFVKESYQWGA
                     R"
     gene            1998..>2950
                     /locus_tag="VFV59_08110"
     CDS             1998..>2950
                     /locus_tag="VFV59_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5325834.1"
                     /translation="MTVTGHRVNRGRVQEIGAREEQAYRDRTPRSAELFGRARESLPL
                     GVASSFQAYDPYPLFMTDARGSRIWDADGNEYIDFDMAFGVLAAGHSHPVLAEALQHR
                     VANGTCYTFPVEDSIRLAEEIKRRFDADMVRFSNSGTEATMDAIRVARGYTGREKVIK
                     MEGGYHGHHDDVLVSIQPPRDAMGPVEAPNTVPASAGIPRSRLAETIVAPFNDADALE
                     AILEAHRGEVAAILVEPVQFNIGVVPPRRGYLERLRELATQHEVVLIFDEVKTGVVLA
                     WGGAYEHYGVKPDLFCLAKSIGGGVPIGAFGGRGEVMRSIES"
BASE COUNT          475 a         1013 c         1046 g          416 t
ORIGIN      
        1 gagcgtgccg aggaggcact cagcctgccg ctcatcgaca acgccatccc cgaggaggcg
       61 gtctggcagt gcaccacctg cggctggtgc gtggagggct gcccggtgct catcgagcac
      121 gtggacacca tcgtcgagat ccgccgcaac gcggtgctgg aggagagtcg cttccccaag
      181 gagctcaacg cggccttccg caatatggag aacgccggca atccgtgggg ccagccgaag
      241 aacgcccgcc tcgactgggc caagggcctg gacgtcccgg tcctgggcca gaccgatacg
      301 cgcctgcgcg cgaacgccaa cggcaacggt gatggcgctg cgggcgcacc ggcgcggtgg
      361 cgcacgatcc acgaatcggc gcccagcggc gacccgatcg gcggccgcgt cctcttctgg
      421 gtgggatgtg ccggcgcctt cgacgatcgc aaccgcaagg tggtgagggc catggcgcaa
      481 ctgctggcgc aggcgggggt ggacttcgcg gtcctcggtg cccaggagac ctgcaccggc
      541 gatccggccc ggcgagcggg gaacgagtac ctgttccaga tgctcgccga ggagaacgtc
      601 gcgaccctct cggccgccca cgccgagcac ggcatctcga ccatcgtcgc gacctgcccg
      661 cactgcttca acacgatccg caacgagtac ccgcagttcg gcctcaacgg cgtccacgtg
      721 ctgcaccaca cgcagctgct ggaccggctg gtggccgagg gtcggctggt cccctcgcag
      781 caccaccagg cgatggtcgc ctaccacgac gcctgctacc tgggccgcta caacgaggtc
      841 tacgacgagg ggcgacgggt ggtcgcctcc gtgcccggcc aggaggtcgt ggagatggac
      901 ctccaccacc gcaagggcat gtgctgcggc gccggcgggg cgcggatgtg gatggaggag
      961 cgcgagggcc atcgcatcaa ccatcggcgc gtggagcagg cgctcgagaa gtcgcctgac
     1021 cagatcgcga ccgcctgtcc cttctgcctg atcatgctgc gggacggcac gatcgacctg
     1081 gaccgtgggg acgtcgaggt gcgcgacgtg gcggagctct tggccgatgc cacgggcgcc
     1141 tggaaccgag aggtggcgac gccgcccgag gcgtgaggtg gcgcgaccgt tcctgcggaa
     1201 tgcgcgtgag cgagtcattc cgccgaacga ggacgctgtt ctcgtgagac gtgccgaatc
     1261 cgatgcaata gctgcagttc gggcggcgct gggccggggg tgagcgtgaa ctccgccctc
     1321 cttgcgcggc attgccggct actgctcgcc aggcgcgcaa tccgcgaaac ggccggcccg
     1381 tgccggcggg cccccaccga ttccgccacc cggcgcggaa tccgcaacaa cgctgcatga
     1441 atcttgcgga aagttgcgca ccccaccgcg ccaccctaga cttcccgcca tggctgtccg
     1501 caacctctcg cagcccgggc gacgccccgt gctcgacgat ctcgacaagg cgatcatcaa
     1561 ggcgctccag ctcgacggtc gccggccgta cgcgcagatc gggcggcagc tcaaggtgcc
     1621 ggaggccacc gtgcggcagc gcgccgagcg gctcatcacc cgcggcgtgg tgcagatcgt
     1681 cggcgtcacc gacccgctct cgatgggctt ccagcagccg gcgctcatcg gcctcaaggt
     1741 ggatgcggga cggctcgaca gcatcgccga gcagatcgcg gcgctggagg aggtgaccta
     1801 cgtcgtcatc accgccggtc ggttcgacat ggtctgcgaa gtcgtgtgcg aagacaacga
     1861 ccatctgctg cgcgtgctga ccgagcagtt cgcgcgcatc gaggggatcc gctcgaccga
     1921 gacgctggtc gagctgcgtt tcgtcaagga aagctaccag tggggcgcgc gttgaggcgc
     1981 cggccaggag gtcgaccgtg acggtcaccg gacatcgggt caatcggggg cgcgtgcagg
     2041 agatcggcgc gcgagaggag caggcgtacc gcgaccggac gccgcgcagc gcggagctgt
     2101 tcggccgggc gcgcgagagc ctgccgctcg gcgtggccag ctcgttccag gcctacgatc
     2161 cctacccact gttcatgacc gatgcgcgcg gctcgcgcat ctgggacgcg gacggcaacg
     2221 agtacatcga cttcgacatg gccttcggcg tgctggcggc cggccactcc cacccggtgc
     2281 tcgccgaggc gctgcagcat cgcgtcgcca acggcacgtg ctacacgttc ccggtcgagg
     2341 acagcatccg gctggccgag gagatcaagc ggcggttcga cgctgacatg gtgcgcttca
     2401 gcaactcggg caccgaggcg accatggacg ccatccgggt ggcgcggggc tacaccggcc
     2461 gcgagaaggt catcaagatg gagggcggct accacggtca ccacgacgac gtgctggtca
     2521 gcatccagcc gccgcgcgac gccatgggcc cggtcgaggc gccgaacacg gtgcccgcca
     2581 gtgccggcat cccgcgcagc cgcctggccg agacgatcgt cgcccccttc aacgatgccg
     2641 atgcgctcga ggcgatcctg gaggcgcacc gcggcgaggt ggcggccatc ctggtcgaac
     2701 ccgtccagtt caacatcggt gtggtgccgc cgcggcgcgg ctacctggag cggctccgcg
     2761 agctggcgac ccagcacgag gtcgtcctga tcttcgacga ggtcaagacc ggcgtcgtgc
     2821 tggcgtgggg cggagcgtac gagcactacg gcgtcaagcc ggacctcttc tgcctggcca
     2881 agagcatcgg cggtggcgtt cccatcggcg ccttcggcgg ccgcggcgag gtgatgcgtt
     2941 cgatcgagtc
//
LOCUS       DASTYP010000180         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1072075, whole genome shotgun sequence.
ACCESSION   DASTYP010000180 DASTYP010000000
VERSION     DASTYP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072075"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..1374)
                     /locus_tag="VFV59_08115"
     CDS             complement(424..1374)
                     /locus_tag="VFV59_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5325835.1"
                     /translation="MTSDLKLGVLAWNQYTDWPALREAGMRADQLGYDSLWTWDHLYP
                     IVGSPEGPMFEGWLTLAAWAERTEKATLGLMVGANTFRNPAVVAKMATTLDHISGGRA
                     VLGIGGAWFETEHRGFGIEFGGSPGERLRWLEEAARIMRGMLHGEEPSGDRFYHAHQV
                     RNDPMPLQDRLPLLIGGGGERKTLRIVARYADACNVGGGFDNVKRKDEILRRHCEEVG
                     RDESEIERTVGMGICVIRDDPAEAQRVANQIFEHNGNADPWKNQLVGTPEQVADAMRP
                     FLGIGFRHFIVGFPSPYDAESMERLATEVRPELTKVPVTA"
     gene            complement(1364..1783)
                     /locus_tag="VFV59_08120"
     CDS             complement(1364..1783)
                     /locus_tag="VFV59_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU5325836.1"
                     /translation="MSDPDLKVQSVLDDELREWLVRELRYPVLAVNSADGPPSQSVMW
                     FDLDPDRPDTILMNTMVRRLKFRQLQEDPRVSLLFEDGLHWVAMRGTVELDATFEPAL
                     QHIQDLARRYHGDPERYAGQERVIIRMKVEKVIRHDQ"
     gene            1873..2988
                     /locus_tag="VFV59_08125"
     CDS             1873..2988
                     /locus_tag="VFV59_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU5325837.1"
                     /translation="MTDAELLSIGAELLLGETVDTNAAFLGAEMARIGLPLQSVRMLP
                     DDRSVIADAFRTARGRSAVVLATGGLGPTHDDLSREGLADALDEALTEDAELRRTLEA
                     RFGGRALMASTNLRQALRVPSAVPLPNPIGSAPGWWVDRDDVVVVLMPGVPSEMRRMF
                     AEEVRPRLLERFGARPLAMRWVKSFGLGESTMAERLGSLLTDPPPGVAAGIYARDDGV
                     HVRFSTSGDRSSLDASVEAAVAILGHDLYGVDDATLPAVALRRLAQLGVGTVSSWESD
                     TEGALLAILAATGAVAGGARYTGGVLDSGTPSGPPVEDACLQLALLPQDANGRSRVRV
                     AVSGAVSMPQVELRVHGSGPQRLRRAAFAALDAVRRM"
BASE COUNT          435 a         1136 c         1076 g          490 t
ORIGIN      
        1 agcgggtggc tgggcgttgt cgggcggccc ggcacgctcg ctcccgctct tccaggggcc
       61 tggcacccgg ctattgcgcg cccggcggca catctcgcga agcgaaggct ggcgaattgc
      121 ggaatgcacg ccagacgcgc atcccgccat ccggcaccct caccgcccgc gaccgagccg
      181 caatatgccc gctggtgaca tcaacgtgcg actcggcgcg gtccgagtcg caatgtgctg
      241 gcggacgagc gcattaggcg gccggcggac cagacgatcg cagcttccac gctcggaagg
      301 cggcgatgag cggcggatcg gccgtctatt gcgtgaatgc tgcggcatat tgcgagtcga
      361 tcggtcccga cagaatcctt gcggtcgccg caccaattgc gggacgcggc gccaccgtcc
      421 gtctcaggcc gtgaccggca ccttggtgag ctcgggccgg acctcggtcg ccagccgctc
      481 catcgactcg gcgtcgtagg gcgacggaaa gccgacgatg aagtgccgga acccgatgcc
      541 caggaacggc cgcatcgcgt ccgcgacctg ctccggcgtc ccgaccagct ggttcttcca
      601 cggatcggcg ttgccgttgt gctcgaagat ctggttggcg acgcgctggg cctcggccgg
      661 atcgtcgcgg atgacgcaga tccccatccc caccgtccgc tcgatctcgg attcgtcgcg
      721 gccgacctcc tcgcagtgcc ggcgcaggat ctcgtccttg cgcttcacgt tgtcgaagcc
      781 gccgcccacg ttgcaggcat cggcgtagcg ggcgacgatt cggagggtct tgcgttcccc
      841 gccaccgccg atcagcagcg ggaggcggtc ctggagcggc atcgggtcgt tgcggacctg
      901 gtgcgcgtgg tagaagcggt cgccggacgg ctcctcgccg tggagcatgc cgcgcatgat
      961 ccgggccgcc tcctcgagcc accgcagtcg ctcacccggc gacccgccga actcgatccc
     1021 aaacccccgg tgctcggtct cgaaccaggc gccgccgatg ccgagcacgg ctcgtccgcc
     1081 gctgatgtgg tcgagcgtgg tcgccatctt ggccaccact gcggggttgc ggaaggtgtt
     1141 ggcgccgacc atgaggccca gcgtcgcctt ctcggttcgc tccgcccagg ccgcgagggt
     1201 cagccagccc tcgaacatcg gcccttccgg cgagccgacg atcgggtaca gatggtccca
     1261 cgtccacagc gagtcgtagc cgagctgatc ggcgcgcatg ccggcctcgc gcagcgcggg
     1321 ccaatcggtg tactggttcc aggcgaggac gcccaacttc aggtcactgg tcatggcgaa
     1381 tcaccttctc gaccttcatg cggatgatca cgcgctcctg cccggcgtag cgctcggggt
     1441 cgccgtggta tcggcgggcg agatcctgga tgtgttgcag cgccggctcg aaggtcgcgt
     1501 cgagctcgac ggtgccgcgc atcgcgaccc agtggagccc gtcctcgaac agcaggctga
     1561 ctcgcgggtc ctcctggagc tggcggaact tcagccgccg caccatggtg ttcatgagga
     1621 tcgtgtcggg acgatccggg tcgaggtcga accacatcac cgactggctc ggaggcccgt
     1681 cggcggagtt cacggcgagc accgggtagc ggagctcgcg caccagccat tcacgcaatt
     1741 cgtcgtcgag gacggactgc accttcagat cagggtcgct catgcatctc ctcgggggca
     1801 agaccgatgc cgcggatggc cgcaatccac cgcatccgct ctgttttcca gtgtagccct
     1861 acactgcgcg ggatgaccga cgccgaactg ctctccatcg gcgccgagct gctgctcggt
     1921 gagaccgtcg acaccaacgc cgcattcctg ggtgccgaga tggcccgcat cgggctgccg
     1981 ctgcagtcgg tgaggatgct gcccgatgac cgatcagtca tagccgatgc attccggacg
     2041 gcccgcggcc gatcggccgt cgtgctggcc accggcggcc tggggcccac acacgacgac
     2101 ctgtcgcgcg agggcctcgc cgatgccctc gacgaggcgc tgaccgagga cgccgagctg
     2161 cggcgcacgc tcgaggcccg attcggcggc cgcgctctca tggcgagcac gaacctgcgc
     2221 caggcactgc gggttccgtc ggccgttccg ctcccgaacc ccatcggctc cgcacccggc
     2281 tggtgggtcg accgcgacga cgtggtcgtc gtgctcatgc ccggcgtccc gtccgagatg
     2341 cgccggatgt tcgccgagga ggtccggccg cgactgctcg agcgcttcgg cgcgcgaccg
     2401 ctcgccatga gatgggtgaa gtccttcgga ctgggcgagt cgaccatggc cgaacggctc
     2461 ggctcgctgc tgacggatcc gccgcccggc gtggcggccg gcatctacgc tcgcgacgac
     2521 ggcgtccacg tccgcttctc cacctccggc gaccgctcct cgctggacgc cagcgtcgag
     2581 gcggcggtgg cgatcctcgg ccacgacctc tacggcgtcg acgacgcgac gctccccgcg
     2641 gtggcgctgc gccgcctggc ccagctcggc gtcggcaccg tgtcctcgtg ggagagcgac
     2701 acggagggag cgttgctcgc catcctcgcc gccaccgggg cggtggccgg tggcgcccgc
     2761 tacaccggcg gcgtgctcga ctctgggacg ccatcgggtc ccccggtcga agacgcctgc
     2821 ctccagctcg cgctgctgcc gcaggacgcg aacggccgat cccgcgtccg agtggcggtc
     2881 agcggcgcgg tgtcgatgcc ccaggtcgag ctccgcgtcc acggctccgg accccagcgt
     2941 ctgcgccgcg ccgccttcgc ggcgctcgac gccgtccgcc gaatgtgagc gtcagcccca
     3001 ggcgccgatg agccggaccg cctgcgggcg gatgtcgaac tgccagatct ctcgcgctcc
     3061 cgcgacgtcg ccgtcggcct ggagcgggat ggggcgaccg tcgagggacc ggatctccac
     3121 gtgcgccgca tcgacgc
//
LOCUS       DASTYP010000181         8607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_453378, whole genome shotgun sequence.
ACCESSION   DASTYP010000181 DASTYP010000000
VERSION     DASTYP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453378"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VFV59_08130"
     CDS             complement(<1..877)
                     /locus_tag="VFV59_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325838.1"
                     /translation="MDVFEDFPLPGMGVADPVVAVPAPGEGVGWWAGSSSAVEDDDGS
                     FVIAYRARLGHAGRGYTVIARSPDGERYETVAEIGQERYGAASMERPALVRTDDGRWR
                     LYVCAASKPPSRHWWIELLEADDPADFATAESRPVFPGDEHTGVKDPLVRRTADGGWE
                     AWICCHPLDERDEEDRMTTAYATSRDGVMWDWHGTVLAPRPGTWDQRGARLTTVLPDG
                     RAAYDGRASKEENWFERTGLARLTGGRPGELEQVSDDAVRDVRYLDVLPLRAGGYLIW
                     YEARLPDESHELRTEL"
     gene            971..1978
                     /locus_tag="VFV59_08135"
     CDS             971..1978
                     /locus_tag="VFV59_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5325839.1"
                     /translation="MNHPARPLPAVAGLRGRVMSVGLGASTARGVGWVLAAALAFATL
                     GTLSGLAYRAGMGSATFVALRAIVGASVLVAVVVVRHRSWVQLRSLPRSEQWMLAAAM
                     AANATLNLALFAAYGEMAVALVLAVYFTYPMLVAVASVLLGRERFTASRSVGLILAGL
                     GVLLVLGDQVGASRFSLVGVGWAVAAAACQATYLVVSRRGYTRVPAEQATALMLGGGA
                     LLAAVVVLAVDLPAGRLTTWTADPGAWLGVITAGVIGAAAAKVWLLRGVRQLGGTRTA
                     VLMLLEPVAGAVLAAVVLAQPLTPIEAVGGVVIIGAATLVQRPAPGPMAPPAPPSTLA
                     A"
     gene            complement(1975..2778)
                     /locus_tag="VFV59_08140"
     CDS             complement(1975..2778)
                     /locus_tag="VFV59_08140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325840.1"
                     /translation="MVVEEVAGGDGAAAALEALLPADVPTRPRALAVLDGLLTGRAWA
                     DDVAAPSWVVVVETGDGTVYVGGEPGRSQLRSLLGQVQTRSGDLVFGFSGPRDPVRAL
                     LPPDPPYVGQAVDFTDRVPPDDEDAMLNGPVPDGLRLVPIDADLLRQTEWYADTLHAF
                     GSEAAWATHGVGRCLLERSRVVAESMAGPRTRGLLEMGVATRGPYRRRGYGTLVSRHV
                     AGACEAAGDRVWWNTAASNLPSQRIARALGFRTERWYDLVAYPPGALRG"
     gene            2872..3192
                     /locus_tag="VFV59_08145"
     CDS             2872..3192
                     /locus_tag="VFV59_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325841.1"
                     /translation="MPDASKALKGATKQVGKAAGAVGDVVGKAAETVGDVVGKVLPGG
                     DGTKRPTRSRSKTASTGSTSSATKSASKGSTSASKSAKSGTSGKTAKATASKAKGTSK
                     RKAS"
     gene            complement(3227..3430)
                     /locus_tag="VFV59_08150"
     CDS             complement(3227..3430)
                     /locus_tag="VFV59_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325842.1"
                     /translation="MRLLELLTRPPLGRGTVPLLVENGRVWCPRRGDIGVDQCRGCPR
                     FVGYDGRELRCRTERRWSVREGH"
     gene            complement(3494..4165)
                     /locus_tag="VFV59_08155"
     CDS             complement(3494..4165)
                     /locus_tag="VFV59_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU5325843.1"
                     /translation="MSSWPRTLWRHPSAVLLGVQLLGVVLYPFMVESPTGRALLSLFA
                     LVVLVMAVRAVRATPALTQVSIGLGVPVVVLTIMEAIRPEDEGILLWSAVLHAAFYFY
                     TSYGLIRYMFKDRHVTPDELFATGATFTVVAWAFAYLYLAVQVVWPGSFTAAVGADLP
                     RTWMEMLFLSFTTLTSTGLSDIAPVLPQARALNMIEQVAGLMYVALVISRIVGLTLYR
                     ERTAD"
     gene            complement(4187..5218)
                     /locus_tag="VFV59_08160"
     CDS             complement(4187..5218)
                     /locus_tag="VFV59_08160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5325844.1"
                     /translation="MRIGYGWLTAQHHPDDRRTDAELYREMIELAVELEASGFDSIWT
                     SEHHFVDDGYMPSQLPVLAAIAARTSRIRIGTGVLLAPLFDPLHVAEDAATVDLISDG
                     RLILGLGAGWREEEFEGFGVPMRERGSRLAGHVAVLRQAWSDGLVTGDGRHFEIPGVN
                     VTPKPMRPGGPPIWIGAGAEAAVRRAGRLADGYLAGPTTPERLRERMAWVREAAEEAG
                     RDPASISANLYRPTFAWRDGDAWARIREAAWYMDWKYGDMGAARGSRIAKRPPPVPSE
                     AEERLRRNTLAGTPEEVAERILEYADVLGADGTYVARLHLPGLEPSVAAEARRIFAEE
                     VLPLVRGAA"
     gene            5261..5686
                     /locus_tag="VFV59_08165"
     CDS             5261..5686
                     /locus_tag="VFV59_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5325845.1"
                     /translation="MEPIALEYRLDCTPEQAWMTYVDRIGEWWHPDYTAAPDRLAGVT
                     IEHGVGGRVYASFTDGTDDRWGRVTAWEPGRRLEHSFTLAQDPAHPSQVSVVFEPDGD
                     GCRMTFEHGGWTDANASSRAKFGDWPLILDRFVRLAQAG"
     gene            complement(5690..6340)
                     /locus_tag="VFV59_08170"
     CDS             complement(5690..6340)
                     /locus_tag="VFV59_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325846.1"
                     /translation="MAVLTPRNSGHVIAVPLAGHACGRLVVNGESTRLTVRGDAPADH
                     LALIRFSGSPAQVESVGDEVSIGFGHGGLFGLRAWLTGEHTEVVLARTVPWVIELRDG
                     VTRLDADLRDLRLRNLAVRGGVSDAELHLPRPHGTVPVMIEGGVTHLTVRIPMGTAAH
                     LELDGVATDLTVDGEQLDAVVGGLTRETAGYADATDRYEITVEGSAARLAIIAGLG"
     gene            complement(6351..6758)
                     /locus_tag="VFV59_08175"
     CDS             complement(6351..6758)
                     /locus_tag="VFV59_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325847.1"
                     /translation="MSTTIVRRVAAALAVGIAALYVLIGLEVLNIGTTASGETPELLD
                     FGLAVGGTYLVIGLMVLFERRRWIWMLAAVIDVLVIAGYFLLADIRHPSFEIWGLLIK
                     ALQGALLVALAVLLVRPRSADEVPGARFAPLHR"
     gene            complement(6901..7143)
                     /locus_tag="VFV59_08180"
     CDS             complement(6901..7143)
                     /locus_tag="VFV59_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325848.1"
                     /translation="MRRLVRAAVIALALSLASVGVTLGHVHGITPLRCLDTDNAHSGA
                     AAAQGDPIPEAGLIPNVLGNANLVPGTLGAASAPCR"
     gene            7219..7461
                     /locus_tag="VFV59_08185"
     CDS             7219..7461
                     /locus_tag="VFV59_08185"
                     /inference="COORDINATES: protein motif:HMM:NF022044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5325849.1"
                     /translation="MLIAALLFLIGLWLIVSNPILGFIPGILLIVAGIVVGVLAMLAR
                     GIGAIASLGLSKTCPQCRSRIPSSATVCRFCGYRDG"
     gene            complement(7648..8037)
                     /locus_tag="VFV59_08190"
     CDS             complement(7648..8037)
                     /locus_tag="VFV59_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5325850.1"
                     /translation="MRIYVTSIFVDDQAKALAFYTEKLGFQKKNDLPAGGARWLTVVS
                     PDAPDGVELLLEPNAHPAAQAYQEALVADGIPCTSFAVDDVQAEHDRLTALGVVFTQP
                     PTEMGPVTTAVFDDTCGNLIQIVKLPA"
     gene            complement(8034..8327)
                     /locus_tag="VFV59_08195"
     CDS             complement(8034..8327)
                     /locus_tag="VFV59_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005152025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5325851.1"
                     /translation="MDDDPLFKALADPTRRTILDELTDRDGQTLFELCSRLAMRHGLG
                     SSRQAISQHLEVLEAAGLVRTHREGRYKFHHLDTAPLREIGERWPQQSKEPDR"
BASE COUNT         1239 a         3098 c         3038 g         1232 t
ORIGIN      
        1 cgagctcggt gcggagctcg tggctctcat ccggcagccg cgcctcgtac cagatcaggt
       61 agccaccggc gcgcagcggc aggacgtcga ggtagcgaac gtcgcgcacc gcgtcgtcgc
      121 tcacctgctc gagctcgccg ggacgcccgc cggtcagccg cgcgagcccg gtccgctcga
      181 accagttctc ctccttgctt gcgcgcccgt cgtacgcggc gcggccatcc gggagaaccg
      241 tggtcaggcg cgcgccgcgc tggtcccatg tgcccggccg cggggcgagg acggttccgt
      301 gccagtccca catcaccccg tcgcgcgagg tcgcgtaggc cgtggtcatc cggtcctcct
      361 cgtcccgctc gtcgagcggg tggcagcaga tccacgcctc ccagccacca tcagcggtgc
      421 gccgcaccag gggatccttg acgccggtgt gctcgtcgcc ggggaacacc gggcgtgatt
      481 ccgccgtggc gaagtcggcg gggtcatcgg cctccagcag ctcgatccac cagtgccggc
      541 tgggcggctt gctggcggcg cagacgtaga ggcgccagcg gccatcatcg gttctgacga
      601 gcgccggacg ctccatggat gccgcgccgt agcgctcctg tccgatctcc gccaccgtct
      661 cgtaccgctc gccatcgggc gaccgcgcga tcacggtgta gccgcggccg gcatgcccca
      721 gccgcgcccg gtacgcaatg acgaaggagc cgtcatcgtc ctccaccgcg ctcgaggagc
      781 cggcccacca gccgacaccc tcgccgggcg cgggcaccgc gacgaccgga tcagcgaccc
      841 ccatcccggg cagcgggaag tcctcgaaca cgtccatcgg gcgactgtac cgcctgaccg
      901 ccagccggca gggtcgattg gggccgcttg gccggtccgc cgcggacgct cgaggagcga
      961 cgctcatcac atgaatcacc cagcccggcc gctgcccgcc gtcgcgggcc ttcgcggtcg
     1021 ggtcatgtcc gtcggcctcg gtgcctcgac ggcacggggc gtcggctggg tcctggccgc
     1081 ggcactcgcc ttcgcgaccc tcggaaccct ctcgggcctc gcgtaccggg cgggcatggg
     1141 atcggcgacg ttcgtcgcgc tgcgggccat cgtgggcgca tcggtcctgg tcgcggtcgt
     1201 cgtcgtccgg caccgcagct gggtgcagct ccggagcctg ccgcgcagcg agcaatggat
     1261 gctcgccgcc gccatggcgg ccaacgcgac cttgaacctg gccctcttcg ccgcctacgg
     1321 cgagatggcg gtggccctcg tgctggcggt gtacttcacc tacccgatgc tggtcgcggt
     1381 cgcatcggtc ctgctcggac gcgagcggtt caccgcgtcg cggtccgtcg gcctcatcct
     1441 cgccgggctc ggagtgctgc tggtcctcgg cgaccaggtc ggcgcgtcgc ggttctcgct
     1501 ggtcggcgtc ggatgggcgg tcgcggcggc cgcgtgccag gccacctacc tggtcgtgtc
     1561 gcggcgtggc tacacgcgcg tcccggcgga gcaggccacg gcgctgatgc tcggcggcgg
     1621 tgcgctcctc gccgcggtgg tcgtgctcgc cgtcgacctg ccggccggcc ggctgacgac
     1681 gtggacggcc gatcccggtg cctggctcgg cgtcatcacg gcgggcgtca tcggtgccgc
     1741 cgcggcaaag gtgtggctgc tgcgcggtgt ccgacagctg ggcgggacgc ggacggcggt
     1801 gctcatgctc ctcgagccgg tggccggcgc ggtcctggcc gcggtcgtcc tggcccagcc
     1861 gctgacgccg atcgaggcgg tcggtggcgt ggtcatcatc ggcgccgcaa ccctggtcca
     1921 gcgaccggcc cccgggccga tggcgccgcc ggcaccaccc tcgactctcg ccgcctagcc
     1981 ccgcagcgcg ccgggtggat acgcgaccaa gtcgtaccag cgctcggtcc ggaaccccag
     2041 ggcccgagcg atccgctggg acggcaggtt cgaggctgcc gtgttccacc acacccggtc
     2101 gccggccgcc tcgcaggcac cggccacgtg gcggctgacc agcgttccgt acccgcggcg
     2161 acgatacggc ccgcgcgtgg ccacgcccat ctcgagcagg ccacgcgtcc tgggtcccgc
     2221 catggactcg gcgaccacgc gcgaccgctc gagcagacac cgcccgacgc cgtgcgtcgc
     2281 ccaggcggcc tcggatccga aggcgtggag cgtatcggcg taccactccg tctgccgaag
     2341 caggtccgcg tcgatgggca cgaggcgcag cccgtcgggt accggcccgt tgagcatcgc
     2401 gtcctcgtcg tcaggcggga cccggtcggt gaagtcgacg gcttggccga cgtagggcgg
     2461 gtcgggcggc agcagggcac gaacgggatc gcgcggcccg ctgaagccga acaccaggtc
     2521 tcccgagcgc gtctggacct ggccgaggag cgaccggagc tgcgaccgcc ccggctcccc
     2581 gccgacgtag accgttccgt cccccgtctc gaccaccacg acccacgatg gcgcggcgac
     2641 gtcgtccgcc caggcgcgac cggtcagcag gccgtcgagc accgccaacg cgcgtgggcg
     2701 cgtcggcacg tcggccggaa gcagcgcctc cagcgctgcc gcggcgccat ccccaccggc
     2761 aacctcctcg accaccatca tcggctccgt cggatgccga gcgaaccggc atgctccgtg
     2821 catcggcgac ggctcggttg ccatgcaagg taccgaatgg gaggtggacc gatgccggat
     2881 gcgagcaaag cgttgaaggg agcgaccaag caggtcggga aggcggccgg cgccgtgggc
     2941 gacgtggtcg gcaaggcagc ggagacggtc ggcgacgtgg tggggaaggt ccttccgggt
     3001 ggggacggca cgaaacgacc gacgcgctcc cgcagcaaga cggcgagcac cggctcgacg
     3061 tcgtctgcca cgaagtcagc gtcgaagggc agcacctcgg cgtcgaagtc ggcgaagtcg
     3121 gggacatcgg ggaagacggc gaaggcgacc gcgtccaagg cgaaggggac gtcgaagcgc
     3181 aaggcgtcct gatcggcgcc gcgccggagg cccccgtccc gagcggtcag tgcccctcgc
     3241 ggacggacca gcggcgttcg gttcggcagc gcagctcccg accgtcgtac ccgacgaagc
     3301 gggggcagcc gcggcactgg tcgacgccga tgtcgccgcg tcgcggacac cacacccgcc
     3361 cgttctcgac gagcagcggc acggtgccgc gtccgagggg cggccgggtc aggagctcga
     3421 gcagcctcat catcggcgca tcgtgcgcca ccgccgcccc gacgcgacag gggcaaaggt
     3481 catcacgcgc cggtcagtcg gccgtgcgtt cccggtacag cgtcagaccg acgatgcgcg
     3541 agatcaccag ggcgacgtac atcagccccg cgacctgctc gatcatgttg agggcgcgcg
     3601 cctggggcag gaccggggcg atgtccgaga gcccggtgct cgtgagcgtc gtgaacgaga
     3661 ggaacagcat ctccatccac gtgcgcggca ggtcggcgcc gacggccgcg gtgaacgatc
     3721 cgggccatac gacctgcacg gccaggtaga ggtaggcgaa cgcccacgcc accacggtga
     3781 aggtggcgcc ggtggcgaac agttcgtccg gtgtcacgtg gcgatccttg aacatgtacc
     3841 ggatcaggcc gtagctggtg tagaagtaga acgccgcgtg caggaccgca gaccagagca
     3901 ggatgccctc gtcctccggc cggatcgcct ccatgatggt gagcaccacc acgggcacgc
     3961 ccaggccgat cgagacctgc gtcagtgccg gcgtcgcgcg gaccgcgcgg acggccatca
     4021 ccagcacgac gagcgcgaac agcgagagca gcgcccgacc ggtcggtgat tcgaccatga
     4081 acgggtagag gacgaccccg aggagctgga cgcccaggag caccgcggat gggtgccgcc
     4141 agagggtccg gggccagctc gacatggcgg ctagggtagc gcggggtcag gctgcgccgc
     4201 ggaccagggg cagcacctcc tcggcgaaga tccggcgcgc ctcggcggcg accgacggct
     4261 ccagtcccgg caggtgcagc cgcgccacgt acgtcccgtc cgcacccagg acgtcggcgt
     4321 actccaggat gcgctccgcc acctcctccg gcgtgccggc cagcgtgttg cgccggagcc
     4381 gttcctcggc ctccgagggc accggtggcg gacgcttcgc gattcgagat ccacgggccg
     4441 cacccatgtc gccgtacttc cagtccatgt accaggccgc ctcacgaatc cgcgcccacg
     4501 catcgccatc ccgccaggcg aaggtgggcc tgtacaggtt ggcgctgatg ctcgccgggt
     4561 cgcgtcccgc ctcctcggcg gcctcacgga cccaggccat gcgctcgcgc agccgctccg
     4621 gggtcgtcgg accggccagg tacccatccg ccaggcggcc ggctcgtcgg acggcggcct
     4681 cggctcccgc gccgatccag atcggcggcc cgccgggccg catcggcttc ggcgtcacgt
     4741 tgacaccggg gatctcgaag tgccggccat cgccggtgac caggccgtcg gaccaggcct
     4801 gtcgcagcac ggccacgtgc ccggccaggc gcgagccccg ctcgcgcatc gggacgccga
     4861 acccctcgaa ctcctcctcg cgccacccgg cgccgagacc caggatcagg cgaccgtccg
     4921 agatcaggtc gacggtggcc gcgtcctcgg cgacgtgcag cgggtcgaag agcggcgcca
     4981 ggagcacgcc ggtgccgatc ctgatgcggc tcgtcctggc cgcgatggcc gcgagcaccg
     5041 gaagctgcga cggcatgtag ccgtcgtcga cgaagtggtg ctcgctcgtc cagatcgagt
     5101 cgaagccgct ggcctccagc tccacggcga gctcgatcat ctcccggtac agctccgcgt
     5161 ccgtgcggcg atcgtcaggg tggtgctggg ccgtcagcca gccgtaaccg atgcgcatgc
     5221 gtgcagccta gcgtcgtcgc gggggcgcta ccctgcggcg gtggaaccca tcgccctcga
     5281 gtaccggctc gactgcacgc ccgagcaggc gtggatgacc tacgtcgatc gcatcggcga
     5341 gtggtggcat cccgactaca cggctgcgcc ggatcggctg gcgggcgtga cgatcgagca
     5401 cggggtgggc ggccgcgtgt acgcctcgtt caccgacggc acggatgacc gctggggtcg
     5461 cgtcaccgcc tgggagcccg gtcggcgcct ggaacacagc ttcacgctcg cccaggatcc
     5521 ggcgcatccc agccaggtgt cggtggtgtt cgaacctgat ggcgatggct gccgcatgac
     5581 cttcgagcac ggcggctgga ccgatgcgaa cgcgtcctcc cgcgccaagt tcggcgactg
     5641 gccgctgatc ctggatcgct tcgtccgact ggcgcaggcc ggctagccct cacccgagcc
     5701 cggcgatgat ggccagcctc gccgcgctgc cctccacggt gatctcgtag cgatccgtcg
     5761 catccgcata gcccgccgtc tcgcgcgtga ggccgccgac gaccgcgtcg agctgctcgc
     5821 cgtccacggt gaggtcggtg gcgacgccgt cgagctccag gtgcgcggcc gtccccatcg
     5881 ggatgcgaac cgtcagatgg gtgacgcctc cctcgatcat caccgggaca gtcccgtgcg
     5941 gccgcggcag gtgcagctcg gcgtcgctca caccgccccg aaccgcgaga ttcctcagcc
     6001 ggaggtcgcg caggtcggcg tccagccggg tgacgccgtc gcgcagctcg atgacccacg
     6061 ggaccgtgcg cgccagcacc acctcggtgt gctccccggt gagccaggcg cgcaggccga
     6121 agaggccgcc atggccgaag ccgatgctca cctcgtcgcc gacgctctcc acctgcgccg
     6181 gcgatcccga gaaccggatc agcgccaggt gatccgccgg tgcatcgccc cggacggtga
     6241 gacgcgtcga ttcaccgttc accaccaacc gtccgcatgc atggccggcc agcggcacgg
     6301 cgatgacgtg gccggagttc ctcggcgtca gtacggccac ggtcgcctcc tcaccgatgc
     6361 agcggtgcga acctggcgcc cgggacctcg tcggccgaac gcggtcgcac cagcaggacc
     6421 gcgagcgcga ccagcagcgc accctgcagc gccttgatca gcagtcccca gatctcgaac
     6481 gacgggtggc ggatatcggc cagcaggaag tagccggcga tgacgagcac gtcgatgacg
     6541 gcggccagca tccagatcca gcgccggcgc tcgaagagca ccatcaggcc gatgacgagg
     6601 taggtcccgc cgacggccag cccgaaatcg agcagctccg gcgtttcgcc cgatgcagtg
     6661 gtgccgatgt tgagcacctc cagcccgatg agcacgtaca gcgccgcgat tcccacggcc
     6721 agcgccgccg ccacgcggcg cacgatcgtc gttgacacgg cgatccctcc aacgtcagcg
     6781 agttcgaatc gtcggaggga tcgtcccgat cccggtcgcg ccgggctaga gccgtgcggc
     6841 ccggtgccga acgggccgca cgtcgtgtcg cgcgccgtgc gacgcgcgcg actcaccgca
     6901 tcagcggcac ggcgctgagg cggcgcccag cgttccaggc acgaggttcg cgttaccgag
     6961 cacgttgggg atgaggcccg cctcgggaat cggatcgccc tgcgccgcgg cagcgccgga
     7021 gtgggcgttg tcggtgtcga ggcacctgag cggcgtgatg ccgtggacgt ggccgagcgt
     7081 cacgccgacc gaggcgagcg agagcgcgag cgcgatgacg gcggcacgaa cgagacgacg
     7141 catggggaac ctcctgcatg tgggtggcga gctacgcccg atcgttcggc cggcgttacg
     7201 cagtacgctg cccgcaccat gctgatcgca gcgctgctgt tcctcatcgg gctgtggctg
     7261 atcgtctcga acccgatcct cgggttcatc cccggcatcc tgctgatcgt cgcggggatc
     7321 gtggtcggcg tgctcgccat gctcgcccgc ggcatcggcg ccatcgccag cttgggcctc
     7381 agcaagacct gcccgcagtg ccgatcacgg attccctcgt ccgcgaccgt ctgccggttc
     7441 tgcggctacc gggacgggtg agccgcttcg cgccggttgc tcgtcagcgg agctaacggc
     7501 gaacttggcg ccgttctgag ccccaccatt cactcgcgtt gcgccaagct gcaaccggcg
     7561 gccaggaaga gcgccgttca agacggtgcg tggccgttag ttccgctgcg gagcaaccgc
     7621 cggccgcggc cgtgggcgcc gcgaccgcta cgccggcagc ttgacgatct ggatcaggtt
     7681 gccgcaggtg tcgtcgaaga cggcggtggt caccggtccc atctcggtcg gcggctgcgt
     7741 gaagaccacg ccgagcgcgg tcagccgatc gtgctcggcc tggacgtcgt cgactgcgaa
     7801 cgaggtgcac gggatgccat cggccaccag cgcctcctgg taggcctggg cggccgggtg
     7861 cgcgttcggc tcgagcagca gctcgacacc gtccggcgca tcgggcgaga cgaccgtgag
     7921 ccatcgggct ccgcctgcgg gcaggtcgtt cttcttctgg aagccgagct tctcggtgta
     7981 gaaggcgagc gccttcgcct ggtcgtcgac aaagatgctg gtgacgtaga tcctcatctg
     8041 tctggctcct tcgattgctg gggccaccgt tcgccgatct cccgcagcgg tgcggtgtcg
     8101 aggtggtgga acttgtatct cccctcccga tgggtgcgga ccaggccggc cgcctcgagc
     8161 acctcgaggt gctgcgagat ggcctgccga gagctcccca ggccgtggcg catcgccagc
     8221 cggctgcaga gctcgaagag cgtctggccg tcgcgatcgg tcagctcgtc gaggatcgtg
     8281 cgccgggtcg ggtctgccag tgccttgaag agtgggtcgt cgtccatcgt ccatcggtgg
     8341 gcgtgcgtga tatgcaagtg taggcttgca tgtcaaccgg ttggcgggcg gttgattcgt
     8401 gtcgcgaatg gaatgcacga gagccgcgcg ttcaggtgcg atctattcgc cacgcgaatg
     8461 agatcgaccg atttcgaccg gtttcgtgtc caaatctgcc gatctgatcc cgctgacgaa
     8521 tgaactgcac cagatcgggc ggattcgggg cgttcctatt cgcccggaga atcgcgcgag
     8581 cctcacccgc ctgccgatcg gcccgcg
//
LOCUS       DASTYP010000182         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_197901, whole genome shotgun sequence.
ACCESSION   DASTYP010000182 DASTYP010000000
VERSION     DASTYP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197901"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..75)
                     /locus_tag="VFV59_08200"
     tRNA            complement(1..75)
                     /locus_tag="VFV59_08200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Gln,seq:ctg)
     gene            complement(164..1096)
                     /locus_tag="VFV59_08205"
     CDS             complement(164..1096)
                     /locus_tag="VFV59_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5325852.1"
                     /translation="MIDKHLPKRLRPAWRVVRRTVANYVDEEGLQWSGAVAFYLVLSV
                     PPLLIAAVSIGVVAVGADSARSFLVDQVAQFLPVERGIVSDIVDQTVDASGAAALVAV
                     AFLLFSGSRVFASLIAAINVMWQEVEPPGFVRRQLQRIGMVFTVGVLLALAGVVDVAI
                     VFTTELLKPPAPIEVLLQSQVVPIVFLGSGLFLLFFLIPRRAATWRTALAGAIIGAVG
                     IRLAQWIFTGYLRQFGGFDSAYGPIAGLAAVLTWALVASAVILLAAHFVAVLNSPDRE
                     PRRQARTDGHQLPGGSEASRRPRPHRRDQQEGSD"
     gene            1249..1761
                     /locus_tag="VFV59_08210"
     CDS             1249..1761
                     /locus_tag="VFV59_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325853.1"
                     /translation="MEQRTLVPGLILIGLGVFLGIVQVTGVGAEAVVAVIGGAFLVTY
                     ALTRNYGFLVPGGIMTGLGLGIIWETQAAANADGVVVIGLGLGFLAIYVIDLLVRGHA
                     ALWWPVIPGGILATIGVLVETEQERWLRGTELLWPAALIVIGGIILLAQIRRGPPETP
                     QPPSPPATQG"
     gene            1954..2337
                     /locus_tag="VFV59_08215"
     CDS             1954..2337
                     /locus_tag="VFV59_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325854.1"
                     /translation="MQQVQPGEWRRFGFGGPPEPWEHGAQRDLDRLATSYYLDILGSR
                     RAILAACRDEALRDRVEELFTTAERHKHEIDYTLRHWATPVERARVEDRLGSLMRAGI
                     RLREIRESLPSSGPISPAPEPMPAA"
     gene            2366..>3165
                     /locus_tag="VFV59_08220"
     CDS             2366..>3165
                     /locus_tag="VFV59_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5325855.1"
                     /translation="MPRLPTLDDVNAARARLRGIALHTPLEPSAALAEACGATEVRLK
                     REDVQPTGSFKTRGAHNKVAVVAEGRPEAALVTASSGNHGIAVATAAMRHDMRLTVLV
                     GRSVSPAKLERLRALETRRITVELTGNDTDDAEAEARRRDDAGHAIYIPPYNDPDVVA
                     GQATVGVEILEDWPSVDAIVVPIGGGGLISGIGLWAKALKPGLRLIGVQPSASPPMYA
                     YFETASTEPMPIAPTLADGVAGNIERASITWQLCRRLVDEVVLVDEEA"
BASE COUNT          481 a         1104 c         1138 g          442 t
ORIGIN      
        1 tggctgggga ggaaggattc gaaccttcgg tctcctgatc cagagtcagg cgccttaccg
       61 cttggccact ccccagtggt cgctcgacgg gcgcatcggc ccggagcctg cggaattgta
      121 gccccatcgg gcgagcaggc caaacgccga tcctgacacc ggcctagtcg ctcccctcct
      181 gttggtcgcg acgatgcggc ctcggccggc ggctcgcctc cgacccccct ggcagctgat
      241 gaccgtccgt cctcgcctgc cgtcgcggct cgcggtcggg cgagttcagc accgccacga
      301 agtgcgcggc gagcaggatc acggccgatg cgaccagcgc ccaggtcagg acggccgcca
      361 gccccgcgat cggtccgtag gccgagtcga agccaccgaa ctggcggagg tacccggtga
      421 agatccattg cgccagccgg atcccgaccg ctccgatgat cgcgccggcg agcgcggtcc
      481 gccaggtggc ggcacggcgc gggatgagga agaagaggag gaagagtccc gaccccagga
      541 agacgatcgg cacgacctgg ctctggagca gcacctcgat cggcgccggc ggcttgagca
      601 gctcggtcgt gaagacgatg gcgacgtcga ccacgccggc cagcgccagc agcacgccga
      661 cggtgaagac catgccgatg cgttgaagct ggcgacgcac gaagcccggg ggctcgacct
      721 cctgccacat cacgttgatg gcggcgatca ggctggcgaa gacgcggctc ccggagaaga
      781 gcaggaaggc cacggcgacg agggcagcgg ccccgctggc gtccacggtc tggtcgacga
      841 tgtccgacac gatcccccgc tccacgggca ggaactgggc gacctgatcc accaggaagc
      901 tgcgcgccga atccgctccg acggccacga cgccgatgga caccgccgcg atcagcagcg
      961 gcgggacaga caggacgagg tagaaggcga cggcccccga ccactggagt ccctcctcgt
     1021 cgacgtagtt cgccaccgtc cgccgaacca cccgccaggc gggccgcagg cgcttcggaa
     1081 ggtgtttgtc gatcatcggg accgcccgag gataccgggc gggtgtccat cggtaccgtg
     1141 ggacggggtt cggatggcac tggtgagcgg agctggtgcc cgggaccctg aagccaccaa
     1201 cgggcgtcgc cgcgtcgccg gcacccgcga ttcggaggtc aaccgaccat ggagcagcgc
     1261 accctggtgc ccggcctgat cctcatcggc ctgggcgtct tcctgggcat cgtccaggtc
     1321 accggagtcg gcgcggaggc ggtcgtggcc gtgatcggtg gtgcgttcct cgtgacgtac
     1381 gccctcaccc gcaactacgg cttcctcgtg ccgggcggca tcatgaccgg cctggggctg
     1441 gggatcatct gggagacgca ggcggctgcg aatgcggacg gcgtcgtcgt catcggcctg
     1501 gggctgggat tcctggccat ctacgtcatc gatctgctgg tccgcggcca cgcggcgctg
     1561 tggtggccgg tgatcccggg cggcatcctg gcgaccatcg gcgtgctggt cgagaccgag
     1621 caggaacgct ggcttcgcgg gacggagctg ctgtggccgg cggccctgat cgtcatcggc
     1681 ggcatcatcc tgctggcgca gatccggcgc ggaccgccgg agacgccgca gccgccgtcc
     1741 ccgcccgcca cgcaggggta gtcccgggtg cctgccggga cgcgtggggt ggcgcccggt
     1801 tcgcatcggt cgtgggcggt ttcgatcggt tcgacggtcg ccgttcgtca ccctggcggt
     1861 agcccgaacg gtcactcccc gagcgagcgg ccgcgccgta gcgtcgtggg cgttgagggc
     1921 acgtccacga cagcgacgga gcaagaacga ccgatgcagc aggtgcagcc cggggagtgg
     1981 cgcaggttcg ggttcggcgg gccgccggag ccctgggagc atggcgcgca gcgagacctg
     2041 gatcgcctcg ccacctcgta ctacctcgac atcctcggct cgcgtcgcgc gatcctcgcg
     2101 gcgtgccggg acgaggcact gcgcgatcgg gtcgaggagc tgttcaccac ggccgagcgg
     2161 cacaagcacg agatcgacta cacgctccgc cactgggcaa ccccggtcga gcgcgcgcgc
     2221 gtggaggacc gacttggctc gctgatgcgt gccgggatcc gcctgcgcga gatccgcgaa
     2281 tcgctgccgt cctccggccc gatcagcccc gcgccggagc cgatgccggc cgcctgacct
     2341 tcccccggct cctacactcg ggcgcatgcc gcgcctgccc accctcgacg acgtcaatgc
     2401 cgctcgcgct cggctgcggg gcattgcgct ccacacaccg ctcgagcctt cggctgcgct
     2461 ggccgaggcg tgcggcgcca ccgaggtccg gctgaagcgc gaggacgtcc agcccaccgg
     2521 ctccttcaag acccgcggcg cgcacaacaa ggtcgcggtc gtcgctgaag gccgacccga
     2581 ggcggcgctg gtcaccgcca gcagcggcaa tcacgggatc gcggtggcca ccgccgcgat
     2641 gcgccacgac atgcggctga ccgtcctggt cgggcgctcc gtgtcgccag ccaagctgga
     2701 gcggctgcgg gcgctggaga cgcgacgcat cacggtggag ctgacgggca acgacaccga
     2761 tgacgccgag gccgaagctc gccgccgcga cgatgccggt cacgccatct acatcccgcc
     2821 gtacaacgac cccgacgtgg tcgccgggca ggcgacggtt ggcgtggaga tcctcgagga
     2881 ctggccgtcg gtggacgcga tcgtcgtgcc gatcggcggt ggtgggctga tctcggggat
     2941 cgggctgtgg gccaaggcct tgaagccggg gctgcggctg attggtgtgc aaccgagtgc
     3001 gtcgccgccg atgtacgcct acttcgagac cgcgtccacc gaaccgatgc ccatcgctcc
     3061 caccctggcc gatggcgtcg ccgggaacat cgagcgcgca tccatcacct ggcagctgtg
     3121 ccggcggctg gtggacgagg tggtgctggt ggacgaggag gccat
//
LOCUS       DASTYP010000183         4526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1326565, whole genome shotgun sequence.
ACCESSION   DASTYP010000183 DASTYP010000000
VERSION     DASTYP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326565"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFV59_08225"
     CDS             complement(<1..558)
                     /locus_tag="VFV59_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEU5325856.1"
                     /translation="MNAPILLYDTTLRDGTQREGLVLSLADKLKIARRLDEAGFPYVE
                     GGWPGSNPKDEEFFAAARGMTFRHAKLAAFGSTRHRANRVEDDPNIRALLAAETPVTT
                     IFGKSWLLHVTDVLGATPEENLAMIGESVAHLRAAGREVVYDAEHFFDGYKADAAYAV
                     ATLRAAIAAGATTVVLCDTNGGCLTD"
     gene            complement(555..1655)
                     /gene="leuB"
                     /locus_tag="VFV59_08230"
     CDS             complement(555..1655)
                     /gene="leuB"
                     /locus_tag="VFV59_08230"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU5325857.1"
                     /translation="MVDHRIAVLPGDGIGPEVCAVARRCVDAAGERHGFRITWDEQPV
                     GGAAMDATGDPLPPATLEAARNADAVLLGAVGGPGWDDPSAPRRPEDALLGLRSALEL
                     FANLRPVVAHPALDAASPLRPEVRAGADVLIVRELTGGLYFGRPQGRSTEDGVEEVVD
                     TLRYTRPEIERIVELAFELAEGRRAHLTSVDKANVLASSRLWREVVEEVAARHPGVTV
                     EHALVDSTAYLLLTRPTRYDVIVTENLFGDILSDEAAAISGSLGLLASASLGTRTTSS
                     GRFGLYEPVHGSAPDIAGTDRANPLATVASAAMLLRWSLGQPDAADRLERAVDAAIAD
                     GPRTADLGGRATTTEVEGFLLERIGAAVGSAA"
     gene            complement(1655..3289)
                     /locus_tag="VFV59_08235"
     CDS             complement(1655..3289)
                     /locus_tag="VFV59_08235"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU5325858.1"
                     /translation="MPRIRVFDTTLRDGEQTPGAALTVDEKVAVAKQLARLNVDVIEA
                     GFPAASPGEAEAVRRISQEVGAAKGAPAIAALARCVPADVTVAAAALAPARKRQLHVF
                     IATSDIHVTHKLRTTRDEVMERIRSCVAQARRMVGPDHDVEFSAEDASRTDLPFLLEA
                     FAAAVEAGASVINVPDTVGYTEPKEYADVVRKVVKLVGRRAIVSTHCHNDLGLATANT
                     LAAIQAGARQVEVTVNGLGERAGNASLEEVVMALRTRPTRFRGATTRVATEQITATSR
                     LVSYLTGIPVQPNKSVVGANAFAHESGIHQDGFLKNPLTYEILTPESVGLSGSTLTLG
                     KLSGRAGFDERLRQLGIHVEPEELDALYAEAISLADQKKQVTDADLLALAEQHADPVP
                     QLVELEHWATASRQGESSSGNVSLFYDGRTFRAFAGGNGPVDALLKAVDNALEQIISE
                     RPRLIDYGLRSVGSGEDAQGEATIKIAAQTGESQTPHVFTGHGLSTNVVEASLRAYLA
                     AVNKLIAEHPAAADVVATARDRRTRSSAPAASGSAR"
     gene            complement(3289..4353)
                     /gene="ilvC"
                     /locus_tag="VFV59_08240"
     CDS             complement(3289..4353)
                     /gene="ilvC"
                     /locus_tag="VFV59_08240"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU5325859.1"
                     /translation="MTKLIYDADIDAAALDGQTVAVIGYGSQGEAHARNLSESGVDVV
                     VGLRPDSASAARAREVGLRVTDAGSATAQANVVMVLVPDTAAPAVYHAEIEPNLRDGS
                     LLMFAHGFNIHFGEIDPPGGIDVGMVAPKGPGHLVRRLYEGGGGVPALYAVHRDATGT
                     ARQRTLAYARGIGAGKAGILETSFAEETETDLFGEQAVLCGGVTSLVQAGFETLVDAG
                     YQPELAYFETMHELKLIVDLMYQGGLAYMRYSISDTAEYGDYVSGPRIVNEATREEMR
                     RILGEIQDGSFARRWIEEGRAGAPEFRRMRAEHATARIEEVGAELRSHMTFIDPKSAP
                     GGWAEGGQAKAEPTPAEGVA"
BASE COUNT          565 a         1660 c         1645 g          656 t
ORIGIN      
        1 gtcggtgagg cagccgccgt tcgtgtcgca cagcaccacc gtcgtcgcgc ccgccgcgat
       61 ggcggcgcgc agggtggcca cggcgtaggc cgcatcggcc ttgtagccgt cgaagaagtg
      121 ctcggcgtcg tagaccacct cgcgacccgc ggcgcgcagg tgggcgaccg actcgccgat
      181 catcgccagg ttctcctccg gcgtcgcgcc gagcacgtcg gtcacgtgga ggagccagct
      241 cttcccgaag atcgtggtga ccggcgtctc ggccgccagg agcgcccgga tgttggggtc
      301 gtcctcgacc cggttggcgc ggtggcgggt cgagccgaag gcggccagct tcgcgtgccg
      361 gaaggtcatg ccccgcgccg ccgcgaagaa ctcctcgtcc ttcgggttgg acccgggcca
      421 gccgccttcc acgtacggga agccggcctc gtccaggcgg cgcgcgatct tcagcttgtc
      481 ggccaggctc aggacgaggc cctcgcgctg cgtgccgtca cgcagggtcg tgtcgtacag
      541 gaggatcggc gcgttcatgc ggcgctcccc accgccgcac cgatgcgctc gagcaggaag
      601 ccctcgacct cggtggtggt ggcgcgtccg cccaggtccg ccgtgcgcgg cccgtcggcg
      661 atggcggcgt cgaccgcccg ctcgagcctg tccgccgcgt ccggctggcc cagcgaccag
      721 cgcagcagca tcgccgccga tgccacggtc gccagcgggt tcgcccggtc cgtgccggcg
      781 atgtccggcg cgctgccatg gaccggctcg tagaggccga accggccgct ggacgtggtg
      841 cgcgtgccca ggctggccga tgccagcagg cccagcgagc cggagatggc ggccgcctcg
      901 tcggagagga tgtcgccgaa caggttctcg gtgacgatca cgtcgtaccg cgtgggtcgc
      961 gtcagcagca ggtaggcggt ggagtccacc agcgcgtgct ccaccgtcac gcccgggtgg
     1021 cgcgcagcca cctcctcgac gacctcgcgc cacaggcggc tcgaggccag cacgttcgcc
     1081 ttgtcgacgc tggtcaggtg ggctcggcga ccctcggcca gctcgaaggc cagctccacg
     1141 atccgctcga tctccggccg ggtgtagcgc agcgtgtcga ccacctcctc cacgccgtcc
     1201 tcggtcgatc gaccctgggg ccggccgaag tagaggccgc cggtcagctc gcgcacgatc
     1261 agcacgtcgg cgccggctcg cacctcggga cgcagcggcg aggccgcatc cagcgcgggg
     1321 tgggcgacga ccgggcggag gttcgcgaac agctccagcg ccgatcgcag gccgagcagc
     1381 gcgtcctccg gccgtcgcgg tgccgagggg tcgtcccagc caggaccacc cacggcgccg
     1441 agcagcacgg cgtcggcatt ccgggcggcc tcgagggtgg ccggcggcag cgggtcaccg
     1501 gtcgcgtcca tggcggcacc gccgaccggc tgctcgtccc aggtgatgcg gaatccgtgg
     1561 cgctcgcccg ccgcgtcgac gcaccggcgc gcgacggcac acacctcggg gccgatcccg
     1621 tcgccgggca ggacggcgat gcggtggtcg accatcaccg ggcgctcccg ctggcggccg
     1681 gcgcgctgga ccgggtccgg cggtcacggg cggtggccac gacgtcggct gccgccgggt
     1741 gctcggcgat cagcttgttc acggccgcga ggtacgcccg caggctggcc tcgaccacgt
     1801 tggtcgagag gccatggccg gtgaacacgt gcggcgtctg cgactcgccc gtctgggcgg
     1861 cgatcttgat cgtcgcctcg ccctgcgcgt cctcgccgga gccgacgctg cgcagcccat
     1921 agtcgatcag cctcggccgc tcgctgatga tctgctcgag cgcgttgtcg accgccttga
     1981 ggagcgcgtc gaccgggccg ttgccgccgg cgaaggcgcg gaacgtccgc ccgtcgtaga
     2041 acagcgagac gttgccgctc gaggactcgc cctgccggct ggcggtcgcc cagtgctcca
     2101 gctcgaccag ctgcgggacc gggtcggcgt gctgctccgc cagggcgagc aggtccgcat
     2161 cggtcacctg cttcttctgg tccgcgaggc tgatggcttc ggcgtagagc gcgtccagct
     2221 cctcgggctc gacgtggatg cccagctggc gcagccgctc gtcgaagcct gcccgacccg
     2281 agagcttgcc gagcgtcagg gtcgagccgg agaggcccac cgactcgggc gtgaggatct
     2341 cgtaggtcag cgggttcttg aggaagccgt cctggtggat gccggactcg tgcgcgaagg
     2401 cattggctcc cacgacgctc ttgttgggct gcaccgggat gccggtcagg tagctgacca
     2461 gccgcgaggt ggcggtgatc tgctcggtcg ccacccgcgt ggtggccccc cggaatcggg
     2521 tcggacgcgt gcggagggcc atcacgacct cctccagcga cgcgttgccg gcacgctcgc
     2581 ccaggccgtt gacggtgacc tcgacctggc gcgccccggc ctggatggca gccagcgtgt
     2641 tcgccgtggc cagccccagg tcgttgtggc agtgcgtgct gacgatcgcg cgccgtccga
     2701 ccagcttgac gaccttgcgc acgacgtcgg cgtactcctt cggctcggtg tagccgacgg
     2761 tgtccggcac gttgatgacc gatgcgcccg cctccacggc cgccgcgaag gcttcgagca
     2821 ggaacggcag gtcggtgcgc gacgcgtcct cggcgctgaa ctcgacgtcg tggtccgggc
     2881 ccaccatgcg ccgcgcctgc gcgacgcacg agcggatgcg ctccatcacc tcgtcgcgcg
     2941 tggtgcgcag cttgtgggtg acgtggatgt cgctggtggc gatgaagacg tgcagctgcc
     3001 gcttccgcgc cggcgccagg gccgcggcag ccaccgtgac gtcggccggg acgcagcggg
     3061 ccagcgcggc gatcgccggc gcacccttcg cggcgcccac ctcctgcgag attcgccgga
     3121 cggcctccgc ctcgcccggg gaggcggccg ggaagccggc ctcgatgacg tcgacgttca
     3181 ggcgggccag ctgcttggct acggccacct tctcgtccac ggtgagcgcc gcgcccggcg
     3241 tctgctcccc gtcgcgcagg gtggtgtcga agacgcggat gcgcggcatc aggcgacgcc
     3301 ctcggccggc gtcggctcgg ccttcgcctg gccgccctcg gcccatcccc cgggcgccga
     3361 cttcggatcg atgaaggtca tgtgcgaccg cagctcggcg ccgacctcct cgatgcgcgc
     3421 cgtcgcgtgc tcggcacgca tccgccggaa ctccggcgcc ccggcgcgac cctcctcgat
     3481 ccagcggcgc gcgaacgagc cgtcctggat ctcgcccagg atccggcgca tctcctcgcg
     3541 cgtcgcctcg ttcacgatcc gcggcccgga gacgtagtcg ccgtactcgg ccgtgtcgct
     3601 gatggagtag cgcatgtacg ccaggccgcc ctggtacatc aggtcgacga tgagcttgag
     3661 ctcgtgcatc gtctcgaaat aggcgagctc cggctggtag ccggcgtcga ccagcgtctc
     3721 gaagccggcc tggaccagcg aggtcacgcc gccgcacagg acggcctgct cgccgaacag
     3781 gtcggtctcg gtctcctcgg cgaagctcgt ctcgaggatg cccgccttgc cggcgccgat
     3841 gccccgtgcg taggccagcg tgcgctgccg cgccgtgcca gtcgcgtcgc ggtggacggc
     3901 gtacagcgcg gggacgccgc cgccgccctc gtacaggcgc cggacgagat gccccggacc
     3961 cttcggcgcc accatgccga cgtcgatgcc gcccggcggg tcgatctcgc cgaagtggat
     4021 gttgaagccg tgagcgaaca tcagcagcga cccatcgcgc aggttcggct cgatctcggc
     4081 gtggtagacc gccggcgccg ccgtgtccgg caccagcacc atgaccacgt tcgcctgcgc
     4141 cgtcgcggag ccggcatccg tcacgcgcag gccgacctcg cgcgcccgcg ccgccgatgc
     4201 ggagtccggc cgcaggccga cgacgacgtc gacgcccgat tcggagaggt tgcgggcgtg
     4261 tgcctcgccc tggctgccgt agccgatgac cgccacggtc tgcccgtcga gggcggcagc
     4321 gtcgatgtcg gcgtcgtaga tgagcttcgt catgcgtcgg gtgctccgtg ctgaactggg
     4381 atggcgacgg cgccagcgcg gaccagggac cgaagtcccc ggccgcgcag cgcctcgatg
     4441 gcgtcgtcga tgcgggacgg tggcgcggcc agccgcgcgc gccaggcgcg cgcatcggtc
     4501 tggacgacct cgccgcccgc ggtggc
//
LOCUS       DASTYP010000184         8799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1692148, whole genome shotgun sequence.
ACCESSION   DASTYP010000184 DASTYP010000000
VERSION     DASTYP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692148"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1169)
                     /locus_tag="VFV59_08245"
     CDS             complement(213..1169)
                     /locus_tag="VFV59_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEU5325860.1"
                     /translation="MSIRALHSRADGRDDLVDLAAWQPRDIPRDELLWVDLVEPTEEE
                     LRTTARALRMDDATCDVMREKPGRPDAHVLADAVQLVVLTPGPDPDDQPIPLAILMGE
                     EWVVTCHPVAIGYLDDHRERVQDQREVGLLSPVQFLASVLDWHIDAFFRVAEALETEV
                     DRLDDAALRTDRDLLRRLVGMRRRISRTRRLLVSHRELFAELRRPDFLPGIEETEAET
                     LRAVGQRLDVATEAVANVREMLIGTFDVHMTRTAQRTNDIMKVLTWVSVILLPAGVLA
                     GVMGMNFKLGFFDDPSMFWVVLGIMVVIAVVTLGIGRWRRWL"
     gene            complement(1187..1738)
                     /locus_tag="VFV59_08250"
     CDS             complement(1187..1738)
                     /locus_tag="VFV59_08250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325861.1"
                     /translation="MAGLRLAVTPTEELTEEQFAELDALCAAAFGHPFAEIWERVGPG
                     VHVIGSVGGRAVAHAMIVDRPLYLGHEADQALDAGYVENVATLPGEQGKGYGGAVMRE
                     VNRLLDEDYALGALATNDNGFYERFHWETWHGATWERNADGERVRTDDKDWHVMILRT
                     RRTPSTLDIGGAIAVDWRPGGSW"
     gene            complement(1747..2490)
                     /locus_tag="VFV59_08255"
     CDS             complement(1747..2490)
                     /locus_tag="VFV59_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5325862.1"
                     /translation="MSALDEVQAALSRGDTDEVLERTDALLRERPGDDAAHELRARAL
                     LVLGRVAEAERHAQDAVRLDPEEIRYRELLAEVLAAGGAHRDAAAEFGRLARNDPGQG
                     EWQVAEAHERIEAAQPGMGVEAAQRAVRLDPTDARAQLELARGLTRTGDARGALQAAT
                     AAASLRPGDAVAREALADARWLADDDAGAFAEFRALAGELRGPDRARVVTKARELYRQ
                     HAGWLGRLLAGIGPLFEAAFMRGWIGLRG"
     gene            complement(2491..3858)
                     /locus_tag="VFV59_08260"
     CDS             complement(2491..3858)
                     /locus_tag="VFV59_08260"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017612822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEU5325863.1"
                     /translation="MPAPNFDVIASLAKRRGFVFPSSEIYGGMAGFWDYGPLGVELRN
                     NVKAAWWRHMVHLRDDVVGLDAAIIMNPRVWEASGHVAGFSDPMVDCRNCKLRFREDD
                     LKGKPSEIACPNCGQRGTLTDARQFNLMFRTHVGPVEESASVAYLRPETAQAMFVQFE
                     NVATTMRRKLPFGIAQIGRSFRNEITPGNFIFRLREFEQMEMEFFVHPRDEDEWFHHW
                     VDARHRWWTDVLGIESARLRLRPHDPDELSHYSKQTTDIEYEFPIGWSELEGVADRTD
                     YDLRAHAAASGKKLELFDAESGEHVVPYVIEPAMGVDRAVLTVLVDGYTEEQLAKETR
                     VVLRIRPDLAPIKAAVLPLLRNRPELVERARRLTADLKQRVTAMYDDTASIGKLYRRQ
                     DEIGTPYCVTVDVDSLEDGAATIRDRDTMTQERVAFDLVAQRVADLVAGRTAWEGVAR
                     EVAEA"
     gene            complement(3949..4809)
                     /locus_tag="VFV59_08265"
     CDS             complement(3949..4809)
                     /locus_tag="VFV59_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325864.1"
                     /translation="MSSDSSATTPNESAAEAPAVPCRPYWPGEHARALRRRILVGLGI
                     GLLAAFVLPSLLRALLLTLTDTPLGEPPAPAVATVVGLIDRFGFSATAILVAWLWVGA
                     RRWDAIEILVWGSQYAAAGYAAVTGIRDPTDAEAAAAWLQANPAPPDEAPEVTYWRAH
                     AQFVSRDLDGARSTVQRLAARADYGFAIASLNAQIAMAQGAHPDLAAVESALPSGSGL
                     ERAIAAANLGALRAQNAWRCGADDVAAARSVRPLMRPHGSNFLLFRMWLPVLGLILVV
                     SVVTALLGTG"
     gene            complement(4823..5605)
                     /gene="recO"
                     /locus_tag="VFV59_08270"
     CDS             complement(4823..5605)
                     /gene="recO"
                     /locus_tag="VFV59_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU5325865.1"
                     /translation="MSRPRVYKTEAIVLRTMDLGEADRVLTVLTPRLGKLRVIAKGVR
                     RPRSRIGGGLQPFSDVQLVLAVGRTFDVVTSSALEDPHLGLRNDLHSTAAAWYLVELA
                     DRFCEGAADSHEAFRLLAQGLSALDAGSEVAREVVARWFELALLDAMGFRPELTRCLE
                     CGAAIEPDGNRYAPASGGTVCPLCAPAATTARPVSADALKVLRHLQRSPLVGVLRLRV
                     PTAVHREVERLLHATVSAVLERELRSRDFLDEVAARREVAPA"
     gene            5702..6751
                     /locus_tag="VFV59_08275"
     CDS             5702..6751
                     /locus_tag="VFV59_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5325866.1"
                     /translation="MDIRTEFTRLLGDIEAEMSDVLDERGGHARPLYDMLAYHLGLDD
                     DGGPRGKRMRPLLGLLAYQSLTGDYHAALPGAAAVELGHNFSLVHDDIEDADRERRHR
                     PTLWAIWGVPLAINAGDALFALSRLALYRLLEGGFSERRVLALMRVYDETCLALCEGQ
                     YLDISFERESRVTVDAYLEMIGKKTAALVGASVQAGAILATDDADTIEAYRRFGYDLG
                     MAFQMADDVKGTFWTSAASGKPVAGDVRKRKKTLPLVWALEHASEADRRRLTEIYEPV
                     RGTDTFAVDGPSAMSDAEVAEVLEVLERCGAREHALTEARRYRDLALAHLDRLPCPPE
                     RRAEMADLVGSVIAA"
     gene            6791..7237
                     /locus_tag="VFV59_08280"
     CDS             6791..7237
                     /locus_tag="VFV59_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEU5325867.1"
                     /translation="MKRLVLWLIAAFVPAAIGAPFPAPDYYRRLNKPSWAPPPSIFGP
                     VWSVLYALIGTSAWLVARRGGSGSRTALGLWWAQLALNAAWTPIFFGARNRGLALVEI
                     LVTWVAILATTVAFFRRRTAAGLLLLPYLAWVTFASALNYAIWRDN"
     gene            complement(7257..8660)
                     /locus_tag="VFV59_08285"
     CDS             complement(7257..8660)
                     /locus_tag="VFV59_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5325868.1"
                     /translation="MDGSTSGNPVLDAAVVIILILVGGFFAASEIALITVKRHRLSQL
                     ADDGSRAARTAQRLTEDPSRFLATIQIAITFLGFLAGAVGARAFSAGLADLLRLIPLG
                     FIRDSAGAIAFVIMTLLIALGSIIVGELVPKTLALNFPERLALVVARPIGWLDRLLSP
                     IVWFVATVSKVVVRLLGGKERPQAGYLSTEELKMLVETGSEQGQIEEEEKEMIHGVIE
                     LAEKRVHEVMVPRIGIRAINVDDSLDEVLDMIVRAGHSRLPVFEESLDNIVGILYAKD
                     LLPYLKNNGRANGQIDVRDLVRPPVYVPESKPVDDLLHEMQVAKRHIAIVVDEYGGTA
                     GLVTMEDVVEEIVGEIQDEYDTEEPMLENLSTDEETVYRLDGRVSMDDLRDLFDLSDE
                     DEPDEEAYDTVGGFVVHRVGRIPLPGAEVPFRDEVTLQVEAAEPRRVAKVVARRPRHQ
                     EERPAADMEEAGERSAG"
BASE COUNT         1245 a         3212 c         3061 g         1281 t
ORIGIN      
        1 ctcggcgagc ccggcgccct tgccgccgag gagggaccgc atggtggcgt tcccctcgct
       61 gaaggtgtag atcagcttgc cgttgctctc cgcactcgcc ttcgcggcgg acttgcgggg
      121 cttgcgcgcg gtcgtggcca tccggagatc gactcctcgt gaatgcgttc gtggggagcg
      181 tcaattgtac cccgagcggg cggttcaacc ggtcagagcc accgcctcca gcggccgatg
      241 cccagcgtca ccaccgcgat cacgaccatg atgccgagga ccacccagaa catgctcgga
      301 tcgtcgaaga agccgagctt gaagttcatg cccatcaccc cggccaggac gcccgcaggc
      361 agcagaatga ccgacaccca ggtcagcacc ttcatgatgt cgttggtccg ctgcgcggtc
      421 cgcgtcatgt gaacgtcgaa ggtgccgatg agcatctcgc gcacgttcgc cacggcctcg
      481 gtggcgacgt cgagccgctg ccccacggcg cgcaacgtct cggcctcggt ctcctcgatc
      541 cccgggagga agtccgggcg ccgaagctcg gcgaacagct cgcgatgcga gacgagcagg
      601 cggcgggttc gcgagatgcg acgccgcatg ccgaccaggc gccgcagcag gtcgcgatcg
      661 gtgcgcagcg ccgcgtcatc cagccggtcg acctcggtct cgagggcctc cgccacgcgg
      721 aagaacgcgt cgatgtgcca atccaggacc gatgccagga actgcaccgg gctcagcaac
      781 cccacctcgc gctggtcctg gacgcgctcg cggtggtcgt cgaggtagcc gatcgcgacg
      841 gggtggcacg tgacgaccca ctcctcgccc atcaggatgg cgagcggaat cggctggtcg
      901 tcggggtccg ggccgggcgt cagcaccacg agctgcacgg catcggccag cacatgcgca
      961 tcgggccggc cgggcttctc gcgcatcacg tcgcaggtgg cgtcgtccat ccggagcgct
     1021 cgggccgtgg tccgcagctc ctcctccgtc ggctcgacca ggtccaccca cagcagctcg
     1081 tcgcggggga tgtcacgcgg ctgccaggcc gcgaggtcga cgaggtcgtc gcgcccgtcc
     1141 gcccgggagt ggagcgccct gatgctcatc gtcgtcgcgg cctcttctac caggacccgc
     1201 cggggcgcca gtccacggcg atcgcgccgc cgatgtcgag cgtcgaggga gtgcggcgtg
     1261 tgcgcaggat catgacgtgc cagtccttgt cgtccgtccg cacgcgttca ccgtcggcgt
     1321 tccgctccca ggtcgcgccg tgccaggtct cccagtggaa ccgctcgtag aagccgttgt
     1381 cgttggtcgc cagggcaccc agcgcgtagt cctcgtccag gaggcggttg acctcgcgca
     1441 tcaccgctcc cccgtaaccc ttgccctgct ccccgggcag ggtcgcgacg ttctccacgt
     1501 accccgcatc gagcgcctgg tccgcctcgt ggcccaggta cagcgggcgg tcgacgatca
     1561 tggcatgagc caccgccctg cctccaacgc tcccgatcac gtggacgccg gggccaaccc
     1621 gctcccagat ttccgcgaag ggatggccga acgccgccgc acacagcgca tcgagctccg
     1681 cgaactgctc ctcggtcagc tcctccgtcg gcgtgacggc gagccgcagc cctgccacca
     1741 ccatcctcag cctcgcaagc cgatccagcc gcgcatgaac gccgcctcga acagtggacc
     1801 gatgccggcc agcaaccgcc cgagccagcc agcgtgctgc cgatagagct cgcgcgcctt
     1861 ggtcaccacc cgggcacggt ccggtccccg cagctcgccc gccagggcac ggaactcggc
     1921 gaaggcgccc gcatcgtcgt cggccagcca gcgggcgtcg gccagcgcct cgcgcgccac
     1981 cgcgtcgccg ggacgcaggc tcgcggccgc ggtcgccgcc tggagggcgc cgcgcgcgtc
     2041 gccggtgcgg gtcagccctc gtgccagctc cagctgcgcg cgagcgtcgg tcgggtcgag
     2101 ccggacggcg cgctgcgccg cctcgacgcc catcccgggc tgcgcggcct cgatccgctc
     2161 gtgcgcctcc gccacctgcc actccccctg gcccggatcg ttacgggcca gccgcccaaa
     2221 ctccgccgcg gcgtcacggt gggctccccc ggccgccagc acctcggcaa ggagctcgcg
     2281 gtagcggatc tcctcagggt ccaggcgcac cgcgtcctgc gcgtggcgct ccgcctccgc
     2341 cacccgcccg agcaccagca gtgcgcgcgc ccgcagctcg tgggcggcat cgtcgccagg
     2401 ccgctcccgg aggagcgcgt cggttcgctc gaggacctcg tcggtgtcgc cgcggctcag
     2461 tgccgcctgg acctcgtcga gggcgctcat ctaggcctcc gcgacctcgc gcgcgacgcc
     2521 ctcccaggcg gtccgtccgg ccacgaggtc cgcgacgcgc tgcgccacca ggtcgaaggc
     2581 gacgcgctcc tgggtcatcg tgtcgcggtc gcggatggtg gccgcgccgt cctccagcga
     2641 gtcgacgtcg acggtgacgc agtacggcgt cccgatctcg tcctggcggc ggtacagctt
     2701 cccgatggag gcggtgtcgt cgtacatcgc cgtcacgcgc tgcttgagat cggcggtcag
     2761 gcgccgcgcc cgctccacca gctccgggcg gttgcgcagc aacggcagga ccgccgcctt
     2821 gatcggcgcc aggtcgggcc ggatccgcag caccacgcgc gtctccttcg ccagctgttc
     2881 ctcggtgtag ccatccacca ggacggtcag caccgcccga tccacgccca tcgcgggctc
     2941 gatcacgtac gggaccacgt gctcacccga ctcggcatcg aacagctcga gcttcttgcc
     3001 tgaggcggcg gcgtgcgcac gaaggtcgta atcggtccgg tcggccacgc cctcgagctc
     3061 cgaccagccg atcgggaact cgtactcgat gtcggtggtc tgcttcgagt agtggctcag
     3121 ctcgtcggga tcatgcgggc gcagccgcag gcgggcgctc tcgatcccca gcacatcggt
     3181 ccaccagcgg tgacgcgcgt cgacccagtg gtggaaccac tcgtcctcgt cacgcgggtg
     3241 gacgaagaac tccatctcca tctgctcgaa ctcgcgcagc cggaagatga agttgccggg
     3301 cgtgatctcg ttgcggaagc tgcggccgat ctgcgcgatc ccgaagggca gctttcgacg
     3361 catggtggtc gccacgttct cgaactggac gaacatggcc tgggccgtct ccgggcgcag
     3421 ataggcgacc gatgccgact cctcgaccgg cccgacgtgc gtccggaaca tgaggttgaa
     3481 ctggcgtgca tcggtcaggg ttccgcgctg gccgcagttg gggcaggcga tctcgctggg
     3541 cttgcccttc aggtcgtcct cccggaagcg aagcttgcag ttgcggcagt cgaccatcgg
     3601 gtcgctgaag ccggcgacgt gaccggacgc ctcccagacg cggggattca tgatgatcgc
     3661 cgcgtccagg ccgaccacgt cgtcgcgcag gtggaccatg tggcgccacc aggcggcctt
     3721 cacgttgttt cgcagctcga cgcccagcgg gccgtagtcc cagaagccgg ccatgccgcc
     3781 atagatctcg ctcgacggga agacgaagcc gcggcgcttc gcgaggctgg cgatgacgtc
     3841 gaagttcggg gctggcacgg tgcgacggcg gctcctggct ggggtggatg cttgaccgat
     3901 gcgggcgctg cgcgcccgac caccgagtct acccgagacg cggccggatc agccggtgcc
     3961 gaggagagcc gtgacgacgc tcacgaccag gatcagtccg aggacgggca gccacatccg
     4021 gaacagcagg aagttcgatc cgtggggacg catcagcggg cgaacgctgc gcgcggcggc
     4081 cacgtcgtcg gcgccgcatc gccacgcgtt ctgcgcgcgg agggcaccga ggttggccgc
     4141 ggcgatcgcc cgctcgagtc cggagcctga cggcagcgcc gattcgacgg ccgcgaggtc
     4201 cgggtgcgcc ccctgggcca tggcgatctg cgcgttgagc gacgcgatcg cgaagccgta
     4261 gtccgcgcgc gccgccaggc gctgcacggt gctccgcgcg ccgtcgaggt cgcggctcac
     4321 gaactgggcg tgggcgcgcc agtacgtgac ctcgggcgct tcgtccggtg gcgcggggtt
     4381 cgcttgcagc catgccgcgg ccgcctcggc gtcggtcggg tcgcggatcc cggtcaccgc
     4441 ggcgtacccg gccgccgcgt actgcgagcc ccacaccagg atctcgatgg cgtcccagcg
     4501 ccgtgcgccg acccacagcc aggcgaccag gatcgcggtt gccgaaaacc cgaagcggtc
     4561 gatgagcccg accacggtcg ccacggcggg agccggcggt tccccgagtg gcgtatcggt
     4621 gagcgtcagc aggagcgccc gcagcagcga aggcaggacg aacgccgcaa ggagcccgat
     4681 gccgagcccg accaggatgc ggcgtcgcag cgcgcgggcg tgctccccgg gccagtacgg
     4741 ccggcacggc accgccgggg cctcggccgc gctctcgttc ggcgtggtcg cggacgaatc
     4801 ggagctcatc tgcgccccgt gcctaggccg gagcaacctc gcgacgggca gcgacctcgt
     4861 ccaggaagtc gcgcgaccgc agctcccgct ccagcacggc ggagaccgtc gcgtgcagca
     4921 gtcgctcgac ctcccggtgc acggccgtcg ggacccgcag cctgagcacg ccgaccagcg
     4981 gcgatcgctg gaggtgccgg agcaccttca gcgcatcggc cgagaccggg cgagcggtgg
     5041 tggcggcggg cgcgcacagc gggcagacgg tgccgccgga ggccggcgcg tagcggttgc
     5101 cgtcgggctc gatggcggcg ccgcactcca ggcagcgggt cagctcgggt cggaagccca
     5161 tcgcgtccag gagcgcgagc tcgaaccagc gcgcgacgac ctctcgcgcg acctcggacc
     5221 cggcgtccag cgccgagagc ccctgggcga gcagccggaa cgcctcgtgc gaatccgccg
     5281 ctccctcgca gaagcgatcc gccagctcga ccaggtacca cgccgccgcc gtggaatgca
     5341 ggtcgttgcg caggccgagg tgcggatcct cgagcgcgga cgacgtcacc acgtcgaagg
     5401 tgcggccgac cgccagcacc agctggacgt cgctgaaggg ctgcagtccg ccaccgatgc
     5461 gggagcgggg ccgccgcacc cccttggcga tcacccgcag cttcccgagc cgcggcgtga
     5521 ggacggtgag cacccgatcc gcctcgccga ggtccatggt gcgcagcacg atcgcctcgg
     5581 tcttgtagac gcgcggccgg ctcaccgggc gacctccggg cccctgaccc atccgaccat
     5641 caccgggcca cgtaccaccg gcatggcgcg gctgatcgag gaggtcgggt acccttccgg
     5701 cgtggacatc aggaccgagt ttacgcggct gctcggcgat atcgaggccg agatgtcgga
     5761 cgtgctcgac gagcgtggtg ggcacgcgcg gccgctgtac gacatgctcg cctaccacct
     5821 cggcctggac gacgacggcg ggccgcgggg caagcgcatg cggccgctgc tgggcctgct
     5881 cgcctaccag tcgctgaccg gcgactacca cgccgcgctc cccggcgcgg ctgccgtgga
     5941 gctgggccac aacttcagcc tcgtgcacga cgacatcgag gatgccgatc gcgagcggcg
     6001 gcatcgcccg acgctgtggg ccatctgggg cgtgcccctg gccatcaacg ccggcgacgc
     6061 cctcttcgct ctgagccggc tcgcgctgta tcggctgctc gaaggcgggt tcagcgagcg
     6121 ccgcgtgctg gcgctcatgc gcgtctacga cgagacctgc ctggccctgt gcgagggcca
     6181 gtacctggac atcagcttcg agcgcgaatc gcgggtgacg gtcgacgcct acctcgagat
     6241 gatcggcaag aagaccgcgg cgctggtggg ggcgtcggtg caggccgggg ccatcctcgc
     6301 caccgacgac gcggacacca tcgaggcgta ccggcgcttc gggtacgacc tcggcatggc
     6361 cttccagatg gccgacgacg tgaaggggac gttctggacg tccgccgcgt ccgggaagcc
     6421 ggtggcgggc gacgttcgca agcgcaagaa gactctgccg ctggtctggg cgctcgagca
     6481 cgcctcggag gcggatcggc gccgcctcac cgagatctac gagccggtcc gcggcaccga
     6541 caccttcgcc gtggacggcc cctccgccat gtccgacgcg gaggtggcgg aggtgctcga
     6601 ggtcctcgaa cgctgcggcg cccgcgagca cgccctgacc gaggcacgcc ggtaccgcga
     6661 cctggcgctg gcccacctgg accggctgcc gtgcccgccg gagcggcgtg cggagatggc
     6721 cgacctcgtc gggtcggtga tcgccgcgta gcgtggccgc gccggcatcg gtcctgcgat
     6781 ggagcccggc atgaagcgcc tggtcctgtg gctgatcgcc gcgttcgtgc ccgccgccat
     6841 cggcgcgccg ttcccggcgc cggactacta ccgtcgcctc aacaagccct cgtgggcgcc
     6901 gccgccgtcg atcttcgggc cggtgtggtc cgtgctgtac gcgctgatcg gcacgtccgc
     6961 ctggctggtc gcgcgccgcg gagggagcgg gagccgaacc gcgctggggc tgtggtgggc
     7021 gcagctggcc ctcaatgccg cgtggacacc gatcttcttc ggcgcgcgca accgggggtt
     7081 ggccctcgtg gagatcctgg tcacctgggt cgccatcctc gccaccacgg tcgcgttctt
     7141 caggcgacgg acggccgccg ggctgctgct gctgccgtac ctggcctggg tgaccttcgc
     7201 gtccgcgctg aactacgcca tctggcgcga caactgaccc acgccggcct gcctcctcag
     7261 ccggcggagc gctccccggc ctcctccatg tccgccgccg gtcgctcttc ctggtgccgc
     7321 ggtcgacgcg ccaccacctt cgccacgcgt cgcggctcgg ccgcctccac ctgtagcgtg
     7381 acctcgtcgc ggaaggggac ctcggccccg gggagcggga tgcgtccgac gcgatgcacc
     7441 acgaagccgc cgacggtgtc gtacgcctcc tcgtccggct cgtcctcgtc cgacaggtcg
     7501 aacaggtcgc gcaggtcgtc catgctgacc cgcccgtcga gacggtagac cgtctcctcg
     7561 tcggtcgaca ggttctcgag catcggctcc tcggtgtcgt actcgtcctg gatctcgccg
     7621 acgatctcct cgaccacgtc ctccatggtc accaggccgg ccgtcccgcc gtactcgtcg
     7681 accacgatcg cgatgtgacg cttggcgacc tgcatctcgt gcagcagatc gtcgaccggc
     7741 ttggactcgg gcacgtagac ggggggccgc accaggtcgc ggacgtcgat ctggccgttc
     7801 gctcgcccgt tgttcttcag gtacggcagc aggtccttgg cgtagaggat gcccacgatg
     7861 ttgtcgaggc tctcctcgaa caccggcagg cgggagtggc cggcgcggac gatcatgtcg
     7921 agcacctcgt ccagcgagtc gtccacgttg atggcccgaa tgccgatgcg cgggaccatc
     7981 acctcgtgaa cgcgcttctc ggccagctcg atgaccccgt ggatcatctc cttctcctct
     8041 tcctcgatct ggccctgctc cgagccggtc tcgacgagca tcttcagctc ctcggtcgac
     8101 aggtagccgg cctgcgggcg ctccttgccg cccaacagcc gcaccacgac cttgctgacg
     8161 gtcgcgacga accagacgat gggcgagagg aggcggtcga gccagccgat cggacgcgcg
     8221 accacgaggg ccaggcgctc cgggaagttc agggccaggg tcttgggcac gagctcgccc
     8281 acgatgatcg agcccagcgc gatgagcagg gtcatgatca cgaacgcgat ggccccggca
     8341 ctgtcccgga tgaagccgag cgggatcagg cgcagcaggt cggcgaggcc cgccgagaag
     8401 gcccgcgcgc cgacggcgcc ggcaaggaag ccgaggaagg tgatggcgat ctggatcgtg
     8461 gccaggaagc ggctgggatc ctcggtcagg cgctgcgccg tgcgggccgc gcggctgccg
     8521 tcatcggcga gctggctgag gcggtggcgt ttgaccgtga tgagcgcgat ctccgacgcg
     8581 gcgaagaagc caccgaccag gatgaggatg atgacaaccg cggcatcgag caccgggttg
     8641 ccgctggtac taccgtccat cgggcggttg tgaccctgcc tccacttcgg gttccgatcc
     8701 ctccgctgcg cggacgggtt cgccggcctc cgacgacgga cgcgctcgac ggcgcatctc
     8761 gatcggccgg gccggcatgc cgacgacggt cttgcccgg
//
LOCUS       DASTYP010000185         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_598981, whole genome shotgun sequence.
ACCESSION   DASTYP010000185 DASTYP010000000
VERSION     DASTYP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598981"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFV59_08290"
     CDS             complement(<1..202)
                     /locus_tag="VFV59_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HEU5325869.1"
                     /translation="MTTHGRVSTDEATRIGELIGLDWASAPFDVEQFRAGMEVELEHG
                     THDLATDVTGDDPVITGKIALAH"
     gene            complement(199..1995)
                     /gene="sulP"
                     /locus_tag="VFV59_08295"
     CDS             complement(199..1995)
                     /gene="sulP"
                     /locus_tag="VFV59_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEU5325870.1"
                     /translation="MSDPEAPTTTAEGPPPLAYRQRRSPFRRRPQEPLLQRAVPVSAQ
                     MPGYRPPTARRDLLAGLTVAALALPSGMAYAEVAGLPPVNGLYALLLPTVLYTLLGSS
                     RHLIVGPEGSIATLVAAAVLPLAAAGSAEASELGAMLALLVAACFLLAWVARLGWLAD
                     YFSRPVLIGYIHGVVVVLICGQLGKLLGLDIEAREPIGQLLEVLRELGDTSAATLLVG
                     AVALAALLAARFVMPALPAALVVVAASILASWAVDLQGEGVAVVGAIPAGLPPVSIPT
                     PSLDDTLKLVPAAIGIFLVGFSDGILTARSFAGKHGQHVRANQELAAFSGLSAAAGVT
                     QGFPLGASGSRTAVNDQMGARTQIAGLTAAATIALVLLFFTEPMQYLPKAVLGAVIVS
                     AAVGLVDLGAWRALRRVSRFELGIAAATTIGVIALGVLEALAIAVALSIVDVVRRSAQ
                     PHDAVLGWVDRLGRYADVQLHPSARIAPGVVIYRLDDRLFFANARYVKGRVLEAVRGA
                     PEPARWLIFDAESVTDVDATGLAMLTELEAALEHDDIEVLVARMKPHIRERLTTDGLA
                     ERIGEDRFFPTVRAAAAACARPEDLQQARSQP"
     gene            complement(2005..>3981)
                     /locus_tag="VFV59_08300"
     CDS             complement(2005..>3981)
                     /locus_tag="VFV59_08300"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU5325871.1"
                     /translation="LSEMLLEHGYSTYMVGKWHLIPSSQETAAGPYDRWPLGRGFERY
                     YGFLGGDTSQWDPDLVYDNHQVEPPRTAEEGYHLTEDLVDKAIEFISDAKQIDPDKPF
                     YLHFCPGATHAPHHVPKEWADRYTGVFDDGWDAYREQVFARQKELGIVPRDAELSRHD
                     PDVPDWGSLSDDQRRLYARMMEVFAGFLSHTDHHIGRLLDHLESIGELENTLIMVISD
                     NGASSEGGPTGTTNEAQFFNNAQEPFEDSLAAIDEIGGPKHFNHYPWGWTFAGNTPFR
                     RWKRETYRGGVSDPFIVSWPKGITATGAVREQYAHAIDMVPTVLDVLGIEPPATIRGV
                     PQSPLHGVSFAHTFDAPTAPSGRTTQYFEMMGHRSIYHDGWRAVCPWPGPSFAEAGKG
                     FGEPISADTLARLDAEGWELYHVAQDPAENYDVAAEHRDRLIALIATWYVEAGKYDVM
                     PVDGSGVTRAAAEKPLVAAPRDRFVYYPGTQSVPFFAGPRVLNRPHSITADVEIPEAG
                     AEGVLLAQGTSAGGFTFYVKDHRLHYTHNWVARELLDIESDAEVGAGRHALRFEFEPT
                     GQPDLAQGHGVPGRLQLYIDGKLAGDREVPYTTPFMFNPGALTCGADPGSAVTTEYQA
                     PFRFTGTIHTVTVDLSGELISDPEAELKMHMARQ"
BASE COUNT          581 a         1361 c         1446 g          593 t
ORIGIN      
        1 ggtgcgcgag cgcgatcttg cccgtgatga ccggatcgtc gcccgtcacg tcggtcgcca
       61 ggtcgtgcgt gccgtgctcg agctcgacct ccatcccggc gcgaaactgc tcgacgtcga
      121 agggcgccga cgcccagtcg aggccgatca gctcaccgat cctcgtggct tcgtcggtgc
      181 tgacacgccc gtgtgtggtc atggttggct cctcgcctgt tgtaggtcct caggcctcgc
      241 gcaggcggcg gcggccgccc ggacggtcgg gaagaaacgg tcctcgccga ttcgctcggc
      301 gagtccgtca gtcgtcaggc gctcccgtat gtgcggcttc atccgcgcga cgagcacttc
      361 gatgtcgtcg tgctcgaggg cggcctcgag ctccgtgagc atcgccagtc cggtcgcgtc
      421 gacgtcggtg acgctctcgg cgtcgaagat cagccagcgc gcgggctcgg gggcgccgcg
      481 gacggcctcg agcactcggc ccttgacgta acgggcgttg gcgaaaaaga ggcggtcgtc
      541 gagccggtag atcacgactc ccggcgcgat ccgggccgac gggtgcagtt gcacgtccgc
      601 gtagcggccg agccggtcga cccagccgag gaccgcgtcg tgcggttgtg cgctgcgccg
      661 gacgacgtcc acgatcgaga gcgcgaccgc gatcgccagc gcctcgagca cgccgagggc
      721 gatgacgccg atcgtggtcg cggcggcgat gccgagctcg aagcggctca cccgccgcag
      781 cgcgcgccat gcgccgaggt cgacgagccc gacggccgcg gagacgatga cggcgccgag
      841 caccgctttg ggcaggtatt gcatcggttc ggtgaagaag agcaggacga gcgcgatcgt
      901 cgccgcggcg gtgaggccgg cgatctgcgt ccgcgccccc atctggtcgt tgaccgccgt
      961 ccgggatccg ctggcgccga gcgggaagcc ctgggtcacg ccggcggcgg cgctgagccc
     1021 ggagaacgcc gcgagctctt ggttggcgcg cacgtgctgg ccgtgcttgc cggcgaacga
     1081 gcgggcggtg aggatgccgt ccgagaagcc gacgaggaag atcccgatcg cggcgggaac
     1141 gagcttcagg gtgtcatcga gcgatggggt ggggatcgac acgggcggca ggccggccgg
     1201 gatcgcgccg acgaccgcca ctccctctcc ctggagatcg acggcccacg aggcgaggat
     1261 cgatgcggcg acgacgacca gcgctgccgg cagcgccggc atgacgaagc gcgcagccaa
     1321 cagcgccgcg agcgcgacag cgccgacgag cagcgtcgcg gcgctcgtgt cgccgagctc
     1381 ccgcagcacc tcgagcagct ggccgatcgg ctcgcgcgcc tcgatgtcca ggccgaggag
     1441 cttgccgagc tgtccgcaga tgagtacgac caccacgccg tggatgtagc cgatgagcac
     1501 gggtcgggag aagtagtccg ccagccaccc gagccgggcc acccacgcga gcaggaagca
     1561 cgcggccacg agcagcgcga gcatcgcgcc gagctccgag gcctcggcgc tgccggcggc
     1621 ggcaagcgga agcacagccg cggcgacgag cgtcgcgatc gagccttcgg gcccgacgat
     1681 cagatgacgc gatgagccca acagcgtgta cagcacggtc ggcagcagca gggcgtagag
     1741 gccgttgacc ggcggaagcc ccgcgacctc ggcgtacgcc ataccggacg gcagtgccag
     1801 cgccgcgacc gtcagtccgg cgagcaggtc gcgccgcgcg gtgggcggtc gatagcccgg
     1861 catctgcgcg ctgacgggaa ccgctcgctg caacagcggc tcctgcggcc gccgacggaa
     1921 cggtgagcgg cgctggcggt aggcgagcgg gggaggcccc tccgcggtcg tcgtcggggc
     1981 ctcagggtcg ctcatcggtc acgcctactg gcgcgccatg tgcatcttca actcggcctc
     2041 ggggtcgctg atcagctcgc cgctgaggtc gacggtcacc gtgtggatgg ttccggtgaa
     2101 gcggaacggt gcctggtact ccgtcgtgac ggccgagccg gggtcagcgc cgcaggtcag
     2161 cgcgccgggg ttgaacatga agggcgtcgt gtacgggacc tcgcggtcgc cggcgagctt
     2221 gccgtcgatg tagagctgga ggcggccggg caccccgtgg ccctgcgcga ggtcgggctg
     2281 tcccgtcggc tcgaactcga agcgcagcgc gtgcctgccc gcgccgacct ccgcgtccga
     2341 ctcgatgtcc agcagctccc gggccaccca gttgtgggtg tagtgcagcc ggtggtcctt
     2401 gacgtagaac gtgaagccac ccgccgaggt cccctgcgcg agcaatacgc cttcggcacc
     2461 cgcctcgggg atctccacgt cggcggtgat gctgtgcggg cggttgagca cgcgcggccc
     2521 ggcgaagaac ggcaccgact gcgtgcccgg gtagtagacg aagcggtcgc gcggcgcggc
     2581 gaccagcggc ttctcggcgg ccgcccgagt gacgccgctg ccgtcgacgg gcatcacgtc
     2641 gtacttgccg gcctcgacgt accaggtcgc gatcagcgcg atcagccggt cacggtgctc
     2701 ggcggccacg tcgtagttct cggcggggtc ttgggcgacg tggtagagct cccagccctc
     2761 cgcgtcgagc cgcgcgagcg tgtcggcgga gatcggctcg ccgaacccct tgccggcctc
     2821 ggcgaacgac gggcccggcc acgggcacac cgcccgccac ccgtcgtggt agatcgaccg
     2881 gtggcccatc atctcgaagt actgcgtcgt gcgcccgctg ggagctgtcg gggcgtcgaa
     2941 ggtgtgcgcg aagctgacgc cgtgcagcgg ggactgcggc acgccgcgga tcgtcgccgg
     3001 cggctcgatg ccgagcacgt cgagcaccgt ggggaccatg tcgatggcgt gcgcgtactg
     3061 ctcgcgcacc gcgccggtcg cggtgatgcc cttcggccag ctgacgatga agggatcgga
     3121 cacgccgccg cggtaggtct ctcgcttcca gcgccggaac ggcgtgttgc ccgcgaacgt
     3181 ccagccccac gggtagtggt tgaagtgctt gggcccgccg atctcgtcga tcgccgcgag
     3241 gctgtcctcg aatggctctt gggcattgtt gaagaactgc gcctcgttcg tcgtcccggt
     3301 cggcccgccc tcggagctgg caccgttgtc cgagatcacc atgatcagcg tgttctcgag
     3361 ctcgccgatg ctctccaggt ggtcgagcag gcgcccgatg tggtggtcgg tgtggctcag
     3421 gaagccggcg aagacctcca tcatgcgcgc atacagccgc cgctgatcgt cggacaacga
     3481 cccccagtcg ggcacgtccg ggtcgtggcg tgacagctcc gcgtcccgcg ggacgatgcc
     3541 gagctccttc tgccgcgcga agacctgctc gcggtaggcg tcccagccgt cgtcgaacac
     3601 ccccgtgtat cggtcggccc actccttggg cacgtggtgt ggcgcgtgtg tcgcacccgg
     3661 gcagaagtgc aggtagaacg gcttgtccgg atcgatctgc ttggcgtcgg agatgaactc
     3721 gatcgccttg tcgaccaagt cctcggtcag gtggtagccc tcctccgccg tccgcggcgg
     3781 ttcgacctga tggttgtcat agaccaggtc gggatcccac tggctcgtgt caccgcccaa
     3841 gaacccgtag tagcgctcga acccgcgccc cagcggccac cggtcatacg ggcccgccgc
     3901 cgtctcctgg ctagaaggga tcagatgcca cttgccgacc atatacgtgc tgtagccgtg
     3961 ctcgagcagc atctccgaca g
//
LOCUS       DASTYP010000186         8366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_672224, whole genome shotgun sequence.
ACCESSION   DASTYP010000186 DASTYP010000000
VERSION     DASTYP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672224"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFV59_08305"
     CDS             complement(<1..275)
                     /locus_tag="VFV59_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325872.1"
                     /translation="MKNEALAMLDWLVGEWDLTMSDAWFMEPGFVGHGTASVEWLGDA
                     FLVLRATLGEEHSTWTWVFGRNDARAQLTVLYHDERGVCRVFALTVE"
     gene            complement(280..1080)
                     /locus_tag="VFV59_08310"
     CDS             complement(280..1080)
                     /locus_tag="VFV59_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5325873.1"
                     /translation="MSDRSLPDSIAANVAEWTETNAQYTDAQAERAWAPQELTWGVFG
                     MLEAAIGSPLGDVNGLDVVELGCGTAYFAAQLARRGARPVGVDPTPAQLATARRAMER
                     TGIVFPLVEAPAERVPLPDRSFDLAFSEFGASLWADPALWIPEAARLLRPGGRLVFLT
                     NSFLSYLCGIDEGGTSETLQRPQVGAYRIQWPGEQGIEYHLPHGRWIELLRGSGFEVE
                     RLIELFAPEGTEPHPYYDNVTPEWASKWPHEEIWVGRLTDEPAAPHSS"
     gene            complement(1135..4488)
                     /locus_tag="VFV59_08315"
     CDS             complement(1135..4488)
                     /locus_tag="VFV59_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5325874.1"
                     /translation="MPRAPRSGTVHRDRARLGGTRQRLIVAITDERRLVTVLFADLVG
                     FTGRAESIDPEEVRELQRSYFSAVAAEAERFGGVVEKYIGDAVMVLFGAPQAHDDDAE
                     RAVHAALAIREALAKLDGELEVRIGINTGEVVGGMAGPMQGDYTVSGDAVNVAARLQQ
                     SAAPNEVLVGAMTRRLAYEAFSFTPHETLPLKGRSEPVETWRVEGALPERRRGRGGEA
                     RLVGRDRELAAAQSALEEAREGRGLMLALVGEAGIGKSRLALEVRQRAETAGFATAWT
                     SSRSYASGVPYHLVGQLLPQLLRAAAGRDMASALRDVGADADEATLRTWARALGDILG
                     TGADTTDAGAPLPPAGRQRFLVQALAAVLRARSAREPMLVVLDDLHWADPASLAVIED
                     LLSTVPELRLLVLATYRSNWSHGWEGRSAYEQLNLRPLRDEDARRLAAELATRSRLTG
                     ELTARVLERAAGNPLFLEELLHVETAGGPDAGPSHRLPASIHEMLLARLDALPPPARR
                     ALQVASVVGMEFSEPLVEHLAETDPTATADALRTLQRAELVVARGGTGDDPTLVFRHP
                     LIHEVAYRSLLTTTRREMHARVARWIEEHGGDDNVAELARHYDHSDDREKARRYLRLA
                     GERSHALNATREALDWFTAAADAWADDPERRAEMLEAATQELHLLGEIERATQMQLDA
                     VRLYEGAGNDRAASRTRIWLGRYYWLLGDPEEAQRQNALAIEGLERHGPSPDLARAYS
                     FRSQILMLVPEFVAAEEWARKAIEVADATGATDVLVHAYNNLGTCLLGRADAAGVTYL
                     RRSRDLALELGLADEVGRANTNLSNQGSRIFPLPYEEMDRHLVEGTAFSARTIPDGIF
                     DRWIRGARGEFLLASGRWAEAEEVLFALDADAAEAYLRSETLTLRAQLFAYRGRYDEA
                     AAMTDGLVETARRVRDMQAVLPVLTTQITIRIGLGDDATAISHLREAVERRSGTGESI
                     ISGWFLFEATDALTALHLRDPSSAALREGVRVLADFCAAFIDDAARGGDLVQVEVRHA
                     LVGAAVEQLAVLARETRVSVTLPTGRLPGRAQALPTLDREHRIFDAARIRLWLAEEGQ
                     DPGELAAAASTFEELAAHPHAERAHRLQG"
     gene            complement(4416..5177)
                     /locus_tag="VFV59_08320"
     CDS             complement(4416..5177)
                     /locus_tag="VFV59_08320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020099249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5325875.1"
                     /translation="MSFEVAAEKYDRFMGRYSRLLAAPMADLAGVESGMRALDVGSGP
                     GALTSELVARLGPERVAAVDPSSPFVEAARARHPGVDVRQASAESLPFDDATFDVALA
                     QLVVHFMADPVGGIGEMRRVTRPDGVVAACVWDYGGGISPLGAFWEAARELQPDLEDD
                     ESRRAGARAGHLTELFEAAGLRDVQETALWVTLEHPSFEAWWEPFEGGVGPAGAFVQS
                     LDAERRDTLRTRCRELLGPAPFTVTARAWAARGSV"
     gene            complement(5174..>8366)
                     /locus_tag="VFV59_08325"
     CDS             complement(5174..>8366)
                     /locus_tag="VFV59_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5325876.1"
                     /translation="YGGTVEKFIGDAVMAVWGAPTAHEDDAERAVRSALDLVSAVQSM
                     GAEIDAPLSLRAGVLTGEAAVTVGATNQGMVAGDLVNTASRLQSAAPPGTVLVGEATR
                     QAASEAIAFEPAGEQLLKGKATPVPAFRALRVVAQRGGVGRSEQLEAPFVGRGPELRM
                     LKDFHAATGAERRPRLVSIIGQGGIGKSRLVWEFLKYIDGVTEVVYWHQGRSPAYGDG
                     ISFWALAEMVRSRATITEADDPVAARTKLAATLDEYVRDDAERRWMEPPLLQLLGLDT
                     SEATERPDRESLFAAWRIFFERIAEHGVVILVFEDLQWADDGLLDFIDHVLEWTRDRP
                     IYLIGLARPELLDRRPGWGAGRRAFTSLVLEPLTADEMRQMLAGLVPGLPPGAVDRII
                     DRAEGIPLYAIETVRMLLATGELILDGGAARPTGDLTDLSVPPSLQALIASRLDALDA
                     DDRSLLQAASVIGKIFSVDALSAVSGRPADEVTQHLRALVRREMLSVDGNPRSPELGQ
                     YGFVQALVREVAYGTLSRRDRRRLHLAAARYFEMLEDEGIAGALAEHYLSAYRAQPDG
                     PEGEAVAAQARVALRGAAERARALGSFSQAVRFLERALEVTTHPDEQRQLHAAAGEAG
                     VLANLAPELPLVHTARALELAREGGDGRHIMDALTGHALALGASGHSAEAAALLEPAR
                     DEYRALSETREYVRLVAMLARATMLRGEGPAALHMIDETLPTAEHLQLNRETVDMLVT
                     RGALLAGLGRVREGVATLVGAVASAESYELTDVGLRGRINLSYAAAPEDPLMAFAVAR
                     EGVEIVNRLGMRGYAGYMLGNATDRAQEIGEWEWAEHALRDALVEGDPDPTVRFRWAV
                     LRGARGEEVEAELAAIRQEAAELTEIQVQATLDEGAADVALAQGNFTRALELARRSYQ
                     HLIAPDSSSLRTAMRAAAWTGSAEILAEVEQRLAEQPGRAPAASRREAQAALAALAGR
                     RAEAASGFAEAMRRWRELGLDVTAAWCAIDWITMLGAGDPAARAAADEARETLERVRS
                     PALLALLDAAVEAQPSPAGGARSPVAEAPAARR"
BASE COUNT         1166 a         3139 c         2935 g         1126 t
ORIGIN      
        1 tcctcgacgg tcagcgcgaa gacgcggcac acgccgcgct cgtcgtggta gaggacggtg
       61 agctgggccc gggcgtcgtt gcggccgaac acccacgtcc aggtcgagtg ctcctcaccc
      121 agtgtggcgc ggagcaccag aaaggcgtca cccagccact ccacgctcgc cgtgccgtgg
      181 cccacgaagc cgggttccat gaaccaggcg tcgctcatcg tcagatccca ctcgccgacg
      241 agccagtcga gcattgccag tgcctcgttc ttcatgatcc taggacgaat gcggcgcagc
      301 cggctcatcg gtcagccgcc cgacccagat ctcctcgtgc ggccacttgg acgcccattc
      361 gggcgtcacg ttgtcgtagt acgggtgcgg ctccgtgccc tccggcgcga acagctcgat
      421 caggcgctcg acctcgaagc cactccctcg cagcagctcg atccaccgtc cgtgcggcag
      481 gtggtactcg atcccctgct ctcccggcca ttgaatccga tacgccccga cctgcggccg
      541 ctgcaaggtc tctgacgtcc ccccctcgtc gatcccgcac aggtacgaga ggaacgagtt
      601 cgtcaggaag accagccggc cgccgggacg cagcagccga gcggcctccg ggatccagag
      661 ggccggatcg gcccagagcg aggcgccgaa ctcggaaaac gcgaggtcga aggaccgatc
      721 cgggagcggc acccgttcgg ccggcgcctc caccaggggg aagacgatgc cggtgcgctc
      781 catggcccgt cgcgcggtcg ccagctgcgc cggcgtcgga tcgaccccaa ccggccgtgc
      841 gcctcgccgc gccagctgag cggcgaagta ggcagtgccg catcccagct cgacgacgtc
      901 gaggccgttg acgtcaccca gcggtgagcc gatggcggcc tccagcatgc cgaagacgcc
      961 ccaggtcagc tcctgcggcg cccaggcgcg ctccgcctgg gcatcggtgt actgcgcgtt
     1021 cgtctccgtc cactcggcga cgtttgcggc gatcgagtcg ggaagcgatc ggtcggacat
     1081 gcagcgcagt gtacgtatgc cgtcgggcga cgccggccgc tggggcccgg ccggtcagcc
     1141 ctgaagccga tgcgcgcgct ccgcgtgcgg atgcgccgcc agctcctcga acgtcgacgc
     1201 cgcggccgcc agctccccgg ggtcctggcc ttcctcggcc agccacagcc ggatgcgggc
     1261 ggcgtcgaag atgcgatgct cgcggtccag cgtgggaagt gcctgggcgc gtcccgggag
     1321 gcgtccggtg gggagggtca ccgagacacg ggtctccctc gccagcacag cgagctgctc
     1381 cacggcggcg ccgacgaggg cgtggcgaac ctcgacctgc accaggtctc cgccgcgggc
     1441 ggcatcgtcg atgaacgcag cgcagaagtc ggccagcacc cgcacgcctt cgcggagggc
     1501 ggccgaggac ggatcgcgca ggtggagcgc ggtcagcgca tcggtcgcct cgaacaggaa
     1561 ccagccgctg ataatggatt cgcccgtgcc ggagcggcgc tcgaccgctt cgcggagatg
     1621 gctgatcgcc gtggcgtcat cgcccaggcc gatgcggatc gtgatctggg tggtcaacac
     1681 cggcagcacg gcctgcatgt cccgcacacg ccgggccgtc tccaccagcc cgtccgtcat
     1741 cgcggccgcc tcgtcgtacc ggccgcggta ggcgaagagc tgcgcgcgca gggtcaacgt
     1801 ctcgctgcgc aggtacgcct cggccgcgtc cgcgtccagg gcgaacagca cctcctccgc
     1861 ctccgcccag cgtcccgacg ccaggaggaa ctcgccgcga gccccgcgaa tccaccgatc
     1921 gaagatgccg tcggggatgg tgcgtgccga gaaggccgtt ccctcgacga ggtgccggtc
     1981 catctcctcg taggggaggg ggaagatccg cgatccctgg ttggagaggt tcgtgttggc
     2041 gcgaccgacc tcgtcggcca gaccgagctc gagtgccagg tcgcggctgc ggcgaaggta
     2101 ggtgacgccc gccgcatcgg cccgcccgag gaggcacgtg ccgaggttgt tgtaggcgtg
     2161 gaccagcacg tccgtcgcac cggtggcatc ggccacctcg atcgccttgc gggcccattc
     2221 ctcggccgcc acgaactccg ggacgagcat caggatctgg gagcggaagc tgtaggcccg
     2281 cgccaggtcg gggctcggcc cgtgacgctc gagcccctcg atggcgagcg cgttctggcg
     2341 ctgagcctcc tccggatccc cgagcagcca gtagtagcgt cccagccaga tccgggtccg
     2401 cgacgcggca cggtcgttcc cggcgccctc gtagagtcgc acggcgtcca gctgcatctg
     2461 cgtggcacgc tcgatctcgc cgaggaggtg gagctcctgc gtcgctgcct ccagcatctc
     2521 cgcgcgccgt tccggatcgt cggcccacgc gtcggccgcg gcggtgaacc agtcgagggc
     2581 ctccctcgtg gcgttcaggg cgtgggagcg ctcaccggcg aggcgcaggt agcggcgcgc
     2641 cttctcccgg tcgtcggagt ggtcgtagtg ccgcgccagc tcggccacgt tgtcgtcacc
     2701 gccgtgctcc tcgatccatc gcgcgacccg agcgtgcatc tcgcggcggg tggtcgtcag
     2761 caggcttcgg taggcgacct cgtggatgag cgggtggcgg aagacgaggg tcgggtcgtc
     2821 gccggtgcca cctcgagcca ccaccagctc cgcgcgctgc agcgtgcgaa gcgcatcggc
     2881 ggtggcggtc ggatcggtct ccgccaggtg ctcgaccaag ggctcggaga actccatgcc
     2941 caccacggat gcgacctgca gcgcgcgtcg ggcgggcggc ggcagcgcgt ccaggcgcgc
     3001 gagcagcatc tcgtggatgg atgccggcag gcggtgtgac ggcccggcgt ccggtccgcc
     3061 ggcggtctcg acgtgcagca gctcctccag gaacagtggg ttaccggccg cgcgctcgag
     3121 gacgcgagcg gtgagctccc ccgtgagccg cgaccgagtc gcgagctccg ctgccaaccg
     3181 acgcgcatcc tcatcgcgca gcggccgcag gttcagctgc tcgtaggcgc tgcggccctc
     3241 ccagccgtgc gaccagttgg accggtaggt ggccaggacc agcaggcgca gctccgggac
     3301 cgtgctcagg aggtcctcga tgaccgccag gctcgccgga tccgcccagt gcaggtcgtc
     3361 gagcacgacc agcatcggct cccgcgcgga cctcgcgcgc agcacggcgg cgagggcctg
     3421 gaccaggaag cgctgccggc ccgccggcgg aagcggtgcg ccggcatcgg tcgtgtcggc
     3481 accggtgccc aggatgtcgc cgagcgcccg cgcccacgtc cggagcgtcg cctcgtccgc
     3541 atcggctccg acatcgcgca gggcggacgc catgtcccgt ccggcggcag cccgcaggag
     3601 ctggggaagg agctgtccca ccaggtggta gggaacgcca gaggcatacg atcgcgacga
     3661 ggtccacgcc gtcgcgaagc cggccgtctc cgctcgctgc cggacctcga gcgccagccg
     3721 gctcttgccg atccccgcct cccccaccag cgccagcatc aggccccgcc cctcgcgcgc
     3781 ttcctccagc gccgactgcg ccgccgccag ctcgcggtcg cgtccgacca ggcgggcttc
     3841 gcccccacgc cctcgccgcc gctccggcag tgccccctcg acgcgccagg tctcgacggg
     3901 ctcgctccgg cccttcagcg gcagggtctc gtggggggtg aaggagaagg cctcgtaggc
     3961 gaggcggcgg gtcatggcgc cgaccaggac ctcgttgggc gccgcgctct gctgcagtcg
     4021 cgccgcgacg ttcaccgcat caccgctgac cgtgtagtcg ccctgcatcg ggccggccat
     4081 cccgcccacc acttcgccgg tgttgatgcc gatcctcacc tcaagctcgc catccaactt
     4141 ggccagcgcc tcgcggatgg cgagggccgc gtgaacggca cgctccgcgt catcgtcgtg
     4201 cgcctggggc gccccgaaga ggaccatcac cgcgtcgccg atgtacttct ccacgacgcc
     4261 cccgaagcgc tcggcctcgg ccgccaccgc cgagaagtag gaccgctgca gctcccgaac
     4321 ctcctcgggg tcgatcgact cggcgcggcc ggtgaagccg accaggtcgg cgaacaggac
     4381 cgtgaccagc cgacgctcgt ccgtgatggc cacgatcaga cgctgccgcg tgccgcccag
     4441 gcgcgcgcgg tcacggtgaa cggtgccgga ccgaggagct cgcggcatcg ggtgcgcagg
     4501 gtgtcgcggc gttccgcatc gagggactgg acgaaggcgc cggccggccc gacgccgccc
     4561 tcgaacggct cccaccacgc ctcgaacgac ggatgctcga gcgtcaccca cagcgcggtt
     4621 tcctgcacgt cacggaggcc ggcagcctcg aacagctcgg tcaggtgccc ggcccgcgct
     4681 ccggcccgcc gcgactcgtc gtcctcgagg tcgggctgga gttcccgcgc cgcctcccag
     4741 aaggcgccga gcggcgagat gccgcccccg tagtcccaga cgcaggccgc gaccacgccg
     4801 tccggccgcg tgacgcggcg catctcgccg atgcccccca ccggatcggc catgaagtgg
     4861 acgaccaact gggccagcgc gacgtcgaag gtcgcgtcgt cgaacggcag cgactcggcc
     4921 gatgcctgcc ggacgtcgac gccggggtgc cgggcacgcg ccgcctcgac gaatggcgac
     4981 gaggggtcga cggcggccac gcgctcaggc ccgaggcgcg cgaccagctc ggaggtgagg
     5041 gcacccgggc cggaccccac atccagcgct cgcatgcccg attcgacgcc ggccaagtcg
     5101 gccatcggcg cggcgagcag gcgggagtat cggcccatga agcggtcgta cttctcggcg
     5161 gcgacctcga agctcatcgg cgcgcggcgg gggcctcggc gaccggcgat cgggcgccgc
     5221 ccgccgggga gggctgggcc tcgacggccg cgtccaggag agcaagcagc gccggcgatc
     5281 gcacccgttc cagggtctcc cgggcttcgt cggccgcggc ccgtgccgcc gggtcgcccg
     5341 cgccgagcat cgtgatccag tcgatcgcgc accaggcggc ggtgacatcg agcccgagct
     5401 ctcgccagcg gcgcatggcc tcggcgaacc cgctggcggc ctccgcgcgg cgcccggcga
     5461 gggcggcgag tgccgcctgg gcctcgcgtc gcgacgcggc gggcgcccgt ccgggctgct
     5521 ccgcgagccg ctgctccacc tcggccagga tctcggcact cccggtccag gcggcggcgc
     5581 gcatcgccgt tcgcagcgac gagctgtccg gcgcaatcag gtgctggtag gaccgccgcg
     5641 cgagctccag tgcgcgtgtg aagttgccct gggccagcgc gacgtccgcg gctccttcat
     5701 ccagcgtcgc ctgcacctgg atctccgtca gttcagccgc ctcctggcgg atggccgcaa
     5761 gttcggcctc gacctcctcg ccgcgtgcgc cacggagcac cgcccaacgg aaccgcacgg
     5821 tcggatccgg atcaccctcg accagggcgt cgcgcagcgc atgctcggcc cattcccact
     5881 ccccgatctc ctgcgcgcgg tccgtggcgt tcccgagcat gtacccggcg tacccacgca
     5941 ttccgagccg attgacgatc tcgacgccct cgcgggcgac ggcgaacgcc atcagcggat
     6001 cctcgggcgc ggctgcgtag ctcaggttga tgcgcccgcg caagccgaca tcggtcagct
     6061 cgtacgactc cgcggatgcc accgcgccga ccagggtcgc gacgccctcg cgcacgcgcc
     6121 cgaggccggc cagcaacgcg ccgcgcgtca cgagcatgtc caccgtctcc cggttcagct
     6181 ggaggtgctc cgcggtcggc agggtctcgt cgatcatgtg cagcgcagcc ggaccctcac
     6241 cgcggagcat ggtggcgcgc gcgagcatcg ccaccagccg gacgtactcg cgcgtctcgc
     6301 tcagcgcgcg gtactcgtcc cgcgccggtt ccagcagggc agcggcctcc gccgagtgac
     6361 cgctcgcccc gagcgccagg gcgtggccag tcagcgcgtc catgatgtgc cgtccgtccc
     6421 cgccctcgcg ggcaagctcc agcgcgcgtg ccgtgtgcac cagcggcagt tccggggcga
     6481 gattggccag gacgccagcc tcccccgccg ccgcgtgaag ctggcgctgt tcgtccggat
     6541 gggtcgtgac ctccagcgcg cgctcgagga agcggacggc ctggctgaag gatcccaggg
     6601 cgcgggcgcg ttccgccgcg ccgcgcagcg ccaccctggc ctgggcggcg acggcctctc
     6661 cctcggggcc gtccggctgc gcgcggtagg cggacaggta gtgctcggcc agggcgcccg
     6721 cgatgccctc gtcctcgagc atctcgaagt accgggccgc ggccaggtgc aggcgacgac
     6781 ggtcgcgtcg cgacagggtg ccgtatgcca cctcccgcac cagcgcctgg acgaagccgt
     6841 actggcccag ctccggcgat cgcgggttgc cgtccacgga cagcatctcg cgccgcacga
     6901 gcgcgcgcag gtgctgcgtc acctcgtcgg ccggacggcc gctcaccgcg ctcagcgcgt
     6961 cgacggagaa gatcttgccg atgaccgatg ccgcctgcag gaggctgcga tcgtccgcgt
     7021 cgagcgcatc cagtcgagag gcgatgagcg cctggagcga gggcggcacc gagaggtcgg
     7081 tcaggtcgcc ggtcgggcgg gccgcaccac cgtcaaggat cagctccccg gtggccagca
     7141 gcatgcggac ggtctcgatc gcgtacagcg ggatgccctc ggcacggtcg atgatccgat
     7201 cgaccgcccc cggcggcagg cccggaacga gcccggccag catctggcgc atctcgtcgg
     7261 cggtgagcgg ctcgaggacc agggaggtga aggcgcggcg accggccccc cagccgggcc
     7321 gtcggtccag gagctccgga cgggcgagac cgatgaggta gatcggccgg tcgcgggtcc
     7381 actcgaggac gtggtcgatg aagtcgagca ggccatcgtc ggcccactgg aggtcctcga
     7441 acaccaggat gacgacgccg tgctcagcga tgcgttcgaa gaagatccgc cacgcggcga
     7501 agagcgactc tcgatcgggc cgttcggtcg cctcactggt gtcgaggccg agcagctgga
     7561 gcagcggcgg ctccatccag cgccgttcgg catcgtcgcg gacgtactcg tcgagcgtgg
     7621 cggccagctt cgtccgcgcg gccaccgggt cgtcggcctc ggtgatcgtg gcccggctgc
     7681 gcaccatctc ggccagggcc cagaagctga tgccgtcgcc gtaggccggc gaccgaccct
     7741 ggtgccagta cacgacctcg gtgacgccgt cgatgtactt caggaactcc cacaccagcc
     7801 ggctcttgcc gatcccgccc tggccgatga tcgacaccag tcgcggacga cgctccgccc
     7861 cggtggcggc gtggaagtcc ttgagcatgc gcagctccgg gcctcggccc acgaacggtg
     7921 cctccagctg ctcgctgcgt cccacgccgc cccgctgggc gaccacccgc agcgcacgga
     7981 aggccggaac cggcgtggcc ttgcccttca gcagctgctc gccggccggc tcgaacgcga
     8041 tcgcctccga agcggcctgc cgcgtcgcct cgccgaccag cacggtgccg ggcggggcgg
     8101 ccgactggag gcgactggcg gtgttcacca ggtcgcccgc caccattccc tggttcgtgg
     8161 cgccgaccgt cacggcagcc tcgccggtca ggacgcccgc tcgcaggctc aggggcgcgt
     8221 cgatctccgc ccccatggac tgcacggcgg agacgaggtc gagcgcgctc cgcaccgctc
     8281 gctccgcgtc gtcctcgtgg gccgttggcg cgccccacac ggccatcacg gcatcaccga
     8341 tgaacttctc caccgtcccg ccgtac
//
LOCUS       DASTYP010000187         6213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_308260, whole genome shotgun sequence.
ACCESSION   DASTYP010000187 DASTYP010000000
VERSION     DASTYP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308260"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /gene="lepA"
                     /locus_tag="VFV59_08330"
     CDS             <1..1350
                     /gene="lepA"
                     /locus_tag="VFV59_08330"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU5325877.1"
                     /translation="IPREEVILASAKEGIGIPEILEAVVHRVPAPRADAAAPLQALVF
                     DSHYDPYKGVVAYVRLAAGTLHDHDRIRFMATAAESEILELGYFRPQPVPTKTLVAGE
                     VGYVATGLKSIREAQVGDTLTSATRGATAPLPGYQEPLPLVFAGIYPLRGDDYPELRD
                     ALDKLHLNDAGFVYEPESSAALGFGFRCGFLGLLHMEIVQERLEREFDLELIASAPSV
                     EYRVKLQHRPEELVIDNPAQMPNIGEIEQISEPWVAAQVITPTDYIGPLMELATGRRG
                     EFQNMEYLDPKRVNLHFEMPLGELIIDFYDQLKSRSSGYASLDYQYLGYRPGDLVKLD
                     VLVNGEPVDALSLIVHRSNAQRQGKALVEKLKELIPRQMFEVPVQAAIGSHIISRETI
                     RAMRKNVLAKCYGGDVTRKRKLLEKQKEGKQRMKRVGSVEIPQDAFLAVLRMNDSAS"
     gene            1357..2019
                     /locus_tag="VFV59_08335"
     CDS             1357..2019
                     /locus_tag="VFV59_08335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU5325878.1"
                     /translation="MSFFDSLRLAIAFGLTLFLVLLRFDSDRIVRSDYFRYRTPWMGP
                     LSYYILVIGFAVGIAVILPSGREQLFLTGGDPDSVAPVTLLFVAVALINGVGLAFLRY
                     GGVLPLPVELLPSRVLGAAANAISEELQFRSIVLGMLLFAGVPMGWAIAVQALVYGLA
                     HRRLWRDRDWYFIAGSVLLGWAAGVATAETGSVIPAIVGHFAVTMSLFAFAGGRLRQR
                     PI"
     gene            2085..3263
                     /gene="hemW"
                     /locus_tag="VFV59_08340"
     CDS             2085..3263
                     /gene="hemW"
                     /locus_tag="VFV59_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU5325879.1"
                     /translation="MSREPHHLYVHVPFCRLVCAYCDFVTVGGRADDIGRYTDALVRE
                     LQLRPAPGQLRTVYFGGGTPSLLDAEQVARVIEAASARWEHSDVDEVTLEANPSSRET
                     PDWAGLRSAGVDRISLGIQSLRDDDLRGLARGHTAAEGRAAFAAVRAAGFDNVSIDLI
                     YGIPGQSLEDWRVGLIAALALEPDHVSTYALQLALQPDEWAAPPRAGALRWRARVAAQ
                     QDEALASEQYRLTEELLNAAGFRHYELSSWARPGHESRHNSAYWARRAYTGIGAGAHS
                     YDGTAERSWNLRDLDRYLQRTEAGERAIEDREELDERTRAFEAIALGLRRVAGMSRRG
                     FAEEFSEDPVDRYAEAVGVSVRAGLLEVDGDMLRLSRDGRLLASEVLLEFAPVPAAAR
                     "
     gene            3374..4477
                     /gene="hrcA"
                     /locus_tag="VFV59_08345"
     CDS             3374..4477
                     /gene="hrcA"
                     /locus_tag="VFV59_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5325880.1"
                     /translation="MTEMREAAPRSAPSRPAFELTDRQRSVLRAVVEDYVLTAVPVGS
                     KALVDRYRLNVSPATVRNAMAELEALGLLTHPHTSAGRVPSDLGYRFYVESLMREAEL
                     DPADRLMIRHQFSQVQLTSNEWHRLAAAILAGATHSASVVTPAKSRRARYGHVQLVAL
                     GDGTRLIVLVMADGNVVQRRLDRSALERQLPDVPVDQAALDSAAIELNAELANLAAPH
                     VRRRATKLRPLAGWIAEAIAGLLEEADGVIVEEVFTDGIVHVLEQPEFSEGTKLRPIL
                     EALQRSDFIEQLVPVLTRGRGVHVIIGRENANDAMHEISLVFAPYGAPDRALGLLGVL
                     GPTRMPYPRAIPTVRYLSTLMNELINSQYGETQ"
     gene            4474..5067
                     /locus_tag="VFV59_08350"
     CDS             4474..5067
                     /locus_tag="VFV59_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5325881.1"
                     /translation="MNDTHHDGHDGGKQHGSTRTRAEERAEQVKAPSRAELLERVRQL
                     ETELETTKAKSEEHLYNWQRSAADFANYKRRTDEDRATITQFGNALLIGKLLGVLDDF
                     DRALENVPAEAHDAWIEGVKLVERKLRGVLESEGVTAIEALNQPFDPNLHEAVAHEAT
                     ADHPDNHVIGEVQRGYKLHDRVIRPSLVRVANNPKEH"
     gene            5075..>6213
                     /gene="dnaK"
                     /locus_tag="VFV59_08355"
     CDS             5075..>6213
                     /gene="dnaK"
                     /locus_tag="VFV59_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU5325882.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPQVITNAEGGRITPSVVAFAK
                     SGERLVGQVAKRQAITNPENTIFSIKRFMGRRFDDPEVQHSKDLVPYEVMKDPKSDGV
                     VVKLANDKTFSPPEISAMILQKLKADAEAYLGEKVTEAVITVPAYFDDTQRQATKDAG
                     RIAGLDVKRIVNEPTASALAYGLDKQKDEKIAVYDLGGGTFDISILELSEGVFEVRST
                     NGDTHLGGDDFDQRVIQWLIDEFRKAEGIDLSKDRIALQRLKEAAEKAKIELSSTTST
                     EINLPFISADATGPKHLVTTLTRAKLEDLVADLINRTADPVKKALADANLQPGDIDEV
                     ILVGGMTRMPAVVEAVKKMFNGKEPHKGVNPDEVVAVGAAIQAGVL"
BASE COUNT         1033 a         2137 c         2149 g          894 t
ORIGIN      
        1 atcccgcgcg aggaggtcat cctggccagt gccaaggagg ggatcggcat ccccgagatc
       61 ctcgaggcgg tcgtccaccg cgtcccggcg ccgcgcgcgg atgccgcggc cccgctgcag
      121 gcgctcgtct tcgacagcca ctacgacccc tacaaggggg tggtcgccta cgtccggctg
      181 gccgccggca cgttgcacga ccacgacagg atccgcttca tggccaccgc ggccgagtcg
      241 gagatcctgg agctcggcta cttccggccg cagcccgtcc cgaccaagac gctggtcgcc
      301 ggcgaggtcg gctacgtcgc caccggcctg aagagcatcc gcgaggcgca ggtcggcgac
      361 acgctgacct cggcgacgcg tggcgcgacc gcgcccctgc cgggctacca ggagccgctg
      421 ccgctcgtct tcgccggcat ctacccattg cgcggcgacg actaccccga gctgcgcgac
      481 gcgctcgaca agctgcacct caacgacgcc ggcttcgtct acgagcccga gtcgtccgcc
      541 gcgctcggct tcggcttccg ctgcggcttc ctggggctcc tgcacatgga gatcgtccag
      601 gagcggctgg agcgcgagtt cgatctcgag ctcatcgcct cggcgccgag cgtcgagtac
      661 cgcgtcaagc tccagcatcg gccggaggag ctcgtgatcg acaacccggc ccagatgccc
      721 aacatcggcg agatcgagca gatcagcgag ccctgggtgg cggcgcaggt gatcacgccg
      781 accgactaca tcggcccgct gatggagctg gcgaccggac gccgcggcga gttccagaac
      841 atggagtacc tcgacccaaa gcgggtcaac ctgcacttcg agatgccgct gggcgagctg
      901 atcatcgact tctacgacca gctcaagagc cgatccagcg gctatgccag cctggactac
      961 cagtacctgg ggtatcggcc cggcgacctg gtgaagctcg acgtgctggt caacggcgag
     1021 ccggtggatg cgctgagcct catcgtgcat cggtccaacg cgcagcggca gggcaaggcg
     1081 ctggtggaga agctgaagga gctgatcccg cggcagatgt tcgaagtgcc ggtccaggcg
     1141 gccatcgggt cgcatatcat cagccgcgaa accatccggg ccatgcgcaa gaacgtcctc
     1201 gccaagtgct acggcgggga cgtgacgcgc aagcgcaagc tcctcgagaa gcagaaggaa
     1261 gggaagcagc ggatgaagcg cgtcggctcg gtcgagatcc cgcaggacgc cttcctggcg
     1321 gtgctgcgca tgaacgacag cgcgtcgtag gggccgatga gcttcttcga ctccctccgc
     1381 ctcgccatcg ccttcggcct gaccctcttc ctggtcctcc tgcgcttcga ctccgatcgg
     1441 atcgtgcggt ccgactactt ccgataccgc acgccgtgga tggggccgct cagctactac
     1501 atcctggtca tcggattcgc cgtcggcatc gccgtgatcc tgccgtcggg gcgcgagcag
     1561 ctgttcctca ccggcggtga cccggacagc gtcgcgccgg tgacgctcct cttcgtcgcc
     1621 gtggccctca tcaacggcgt cggcctcgcc ttcctgcgct acggcggcgt gctgccactg
     1681 ccggtcgagc tgctgccgtc ccgggtcctg ggcgcggccg ccaacgccat cagcgaggag
     1741 ctgcagttcc gctcgatcgt gctcgggatg ctgctcttcg ccggcgtccc gatggggtgg
     1801 gccattgccg tccaggccct cgtctacggg ctcgcccatc gccggctgtg gcgtgaccgc
     1861 gactggtact tcatcgccgg ctccgtcctc ctcggctggg cggccggcgt cgccaccgcc
     1921 gagaccggct cggtcatccc ggccatcgtc ggccacttcg cggtgacgat gtcgctgttc
     1981 gcgttcgccg gagggcgcct gcgccagcga ccgatctagg agcagataca gcaaccatct
     2041 cgcgtcctga atgtcgttgg tgcatggctg acaatgggtt cgccatgtca cgcgagccgc
     2101 accatctcta cgtccacgtg ccgttctgcc ggctggtgtg cgcgtactgc gacttcgtga
     2161 cggtcggcgg tcgggccgac gacatcggcc gctacaccga tgcgctggtg cgcgagctcc
     2221 agctgcggcc ggcgcccggg cagctgcgaa cggtctactt cggcggtgga acgccctcgc
     2281 tgctcgatgc ggagcaggtc gcacgggtga tcgaggccgc ctcggcacgg tgggagcaca
     2341 gcgacgtcga cgaggtgacg ctggaggcca acccgagctc gcgcgagacg ccggactggg
     2401 ccggcttgcg cagcgcgggc gtggatcgga tcagcctggg catccagtcg ctgcgcgacg
     2461 acgacctgcg cggcctcgcg cgggggcaca ccgcggccga agggcgcgcg gcgttcgccg
     2521 cggtgcgagc ggcgggcttc gacaacgtca gcatcgacct gatctacggg attcccggcc
     2581 agtcgctcga ggattggaga gtggggctca tcgccgccct ggcgctcgag ccggatcacg
     2641 tcagcaccta tgccctgcag ctcgcccttc agccggacga gtgggccgca ccgccacgcg
     2701 ccggtgccct ccggtggcga gcgcgcgtcg cggcccagca ggacgaggcg ctcgcatcgg
     2761 agcagtaccg gctgaccgag gagctgctga acgcggccgg cttccgtcac tacgagctca
     2821 gctcgtgggc tcggccgggc cacgaatcgc gccacaacag cgcctactgg gcgcgccggg
     2881 cgtacaccgg catcggtgcc ggagcgcact cgtacgacgg gacggcggag cgctcgtgga
     2941 acctgcggga cctggaccgt tacctccaac ggacggaggc gggtgagcgg gcgatcgagg
     3001 accgagagga gctcgacgag cggacgcggg ccttcgaggc gatcgcactc gggctccggc
     3061 gcgtggcggg catgagtcgt cgagggttcg ctgaggagtt cagcgaggac cccgtcgatc
     3121 ggtacgcgga ggcggttggc gtatcggttc gggcgggcct gctcgaggtc gatggcgaca
     3181 tgttgcgcct ctcgcgggac ggccgattgc tcgcgtcgga ggtgctgctc gagttcgcgc
     3241 cggtgcccgc cgccgcgcgt tgacaggcga attcgggcgg cggtaccatg ctcgccgaag
     3301 cccgttagca ctctccacct gagagtgcca gcacacggga cccgaggacc aaccaccagt
     3361 cgacggaggc gccatgacgg agatgcgtga ggcagcaccg cgttccgcgc cgagccggcc
     3421 ggccttcgag ctgaccgatc ggcagcgatc cgtgctgcgc gccgtcgtgg aggactacgt
     3481 gctcaccgcc gtgccggtcg gctccaaggc gctggtggat cggtaccggc tgaacgtgtc
     3541 gccggccacg gttcgcaacg ccatggcgga gctggaggcg ctggggctcc tgacgcatcc
     3601 gcacacgagc gcgggtcgcg tgccgtccga cctcggctac cgcttctacg tcgagtcgct
     3661 catgcgcgag gcggagctgg atcctgcgga tcggctcatg atccgccacc agttcagcca
     3721 ggtgcagctg accagcaacg agtggcaccg gctggcggcg gccatcctgg ccggggcgac
     3781 ccactccgca tccgtggtga cgccggccaa atcgcgtcgg gcgcgctacg ggcacgtgca
     3841 gctcgtggcc ctcggggacg ggacgcggct gatcgtgctg gtcatggccg acggcaacgt
     3901 cgtccagcgg cgcctggatc ggagcgcgtt ggagcgacag ctgcccgacg tgccggtcga
     3961 ccaggcggcg ctcgactccg ccgccatcga gctgaacgcg gagctggcga acctcgccgc
     4021 accgcacgtc cggcgccggg cgacgaagct gcggccactg gccggctgga tcgccgaggc
     4081 catcgccggc ctgctcgagg aggcggacgg cgtgatcgtg gaggaggtct tcaccgacgg
     4141 catcgtgcac gtcctggagc agccggagtt cagcgagggc accaagctgc ggccgatcct
     4201 cgaggcgctg cagcgctccg acttcatcga gcagctggtc ccggtcctga cgcgtgggcg
     4261 gggcgtacac gtgatcatcg gccgcgagaa cgcgaacgac gcgatgcacg agatcagcct
     4321 ggtcttcgcg ccctacggtg cgccggaccg ggcactcgga ttgctgggag tcctgggacc
     4381 gacgcgcatg ccgtatccac gggccatccc gacggtccgc tacctctcca ccctgatgaa
     4441 cgagctgatc aacagccagt acggcgagac ccaatgaacg acactcatca cgacggccac
     4501 gacggtggca agcagcacgg atcgacgcgg acgcgagccg aggagcgtgc ggagcaggta
     4561 aaggcgccat cgcgcgccga gctgctcgag cgcgtgcgac agctcgagac cgagctggag
     4621 acgacgaagg ccaagtccga ggagcacctc tacaactggc agcgctccgc ggccgacttc
     4681 gcgaactaca agcggcggac cgacgaggac cgcgcgacca tcacgcagtt cggcaacgcg
     4741 ctgctcatcg gcaagctgct gggcgtcctc gacgacttcg atcgcgccct cgagaacgtc
     4801 ccggccgagg cgcacgacgc ctggatcgag ggggtgaagc tggtcgagcg caagctgcgc
     4861 ggcgtcctcg agtccgaggg cgtgaccgcc atcgaggcgc tcaaccagcc gttcgacccg
     4921 aacctgcacg aggcggtggc ccacgaggcg accgccgacc atccggacaa ccacgtgatc
     4981 ggcgaggtcc agcgcggcta caagctgcac gaccgggtca tccggccgag cctcgtgcgc
     5041 gtggcgaaca atccgaagga gcactgacga gacgatgggc aagatcatcg gcatcgacct
     5101 gggcacgacc aactcggtcg tggccgtcat ggaaggcggc gagccccagg tcatcaccaa
     5161 cgcggagggc ggccgcatca cgccatcggt cgtcgccttc gccaagagcg gggagcggct
     5221 ggtcggccag gtcgccaagc ggcaggccat caccaacccc gagaacacga tcttcagcat
     5281 caagcgcttc atgggccgtc ggttcgacga ccccgaggtg cagcacagca aggacctcgt
     5341 gccctacgag gtcatgaagg accccaagtc ggacggcgtc gtcgtcaagc tggcgaacga
     5401 caagaccttc agccctccgg agatcagcgc catgatcctc cagaagctga aggccgatgc
     5461 cgaggcgtac ctgggcgaga aggtgacgga ggcggtcatc accgtcccgg cctacttcga
     5521 cgacacgcag cgccaggcca ccaaggacgc cggccgcatc gccggcctgg acgtcaagcg
     5581 gatcgtcaac gagccgacgg catcggcgct ggcgtacggc ctcgacaagc agaaggacga
     5641 gaagatcgcc gtctacgacc tgggcggcgg cacgttcgac atctcgatcc tcgagctgag
     5701 tgagggcgtc ttcgaggttc gctcgacgaa tggcgacacc cacctcggcg gcgacgactt
     5761 cgaccagcgc gtcatccagt ggctgatcga cgagttccgc aaggccgagg gcatcgacct
     5821 gtcgaaggat cggatcgccc tgcagcgcct gaaggaggcc gccgagaagg cgaagatcga
     5881 gctgtccagc acgacctcga ccgagatcaa tctgccgttc atcagcgccg atgccaccgg
     5941 gcccaagcac ctggtcacga cgctgacgcg ggccaagctc gaagacctgg tcgccgacct
     6001 catcaaccgc acggccgacc cggtcaagaa ggcgctggcc gatgcgaacc tgcagccggg
     6061 cgacatcgac gaggtcatcc tcgtgggtgg catgacccgc atgccggccg tcgtcgaggc
     6121 ggtcaagaag atgttcaacg gcaaggagcc gcacaagggc gtcaaccccg acgaggtggt
     6181 ggcggtcggc gcggcgatcc aggccggcgt cct
//
LOCUS       DASTYP010000188         5027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_781601, whole genome shotgun sequence.
ACCESSION   DASTYP010000188 DASTYP010000000
VERSION     DASTYP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781601"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFV59_08360"
     CDS             <1..195
                     /locus_tag="VFV59_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU5325883.1"
                     /translation="AGVRERVTPHTFRHSFATHLLEGGADLRVVQALLGHASISTTQL
                     YTHLTGERVREVYARAHPRA"
     gene            229..1071
                     /locus_tag="VFV59_08365"
     CDS             229..1071
                     /locus_tag="VFV59_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU5325884.1"
                     /translation="MSYAKNLLSRGEEVVYESRQHWFAVIARTWAWIILGIFALALLI
                     FVVSGSQPIADNETLDGVLTIVAVIGLLLAVGFVGFVLWDWRNQEWLITTRRVMRAEG
                     VLNKSVTDTSLEKINDARLDQSVFGRIFGYGTLDILTAAEEFNGGNVADFPMIADPVQ
                     FKKAMFDQKQMLEQPDLAPPRYQRPAPVPQMERPEPMPPRAGSDRVAVRGDEAREPVT
                     AAAMAPEPEHAALPTPAGAPAAHRSSSEELSATLERLAGLRDKGLITPEEYEAKKRDI
                     LERM"
     gene            1072..1959
                     /locus_tag="VFV59_08370"
     CDS             1072..1959
                     /locus_tag="VFV59_08370"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5325885.1"
                     /translation="MADAPLGALPDGLYGEELDRRISEATGVIAGRLSTEPGLERPRA
                     VAVLGSGLGGVVDLLDAQPRLVMSYAEIPNVPGAGVEGHAGQLIAGHVGGMPMVVLSG
                     RAHPYEGHTQRAATLLLRSVLALGPELVILTNAAGGLNPDFDPGDVMLISDHINLSGE
                     NPLLGPNLDRFGPRFPPMTDAYDPRLRAHALESAERTRIGLRQGVYIMLSGPSYETRA
                     EMRMLRTLGGDAVGMSTAHEVIVARHAGRRVLGFSLITNKATDDVETGATHEEVIEVG
                     RIGAERLVTLLGDLLPRLA"
     gene            1959..2576
                     /locus_tag="VFV59_08375"
     CDS             1959..2576
                     /locus_tag="VFV59_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5325886.1"
                     /translation="MLFSNQPLPYLVAVAIALLVGLTFHEFSHAATADSLGDRRPRAM
                     GRLTLNPLRHLDPIGAAMLLLVGFGWAKPVMVNPYALRNGRQGMAFVAAAGPLSNVVV
                     ALLFGVIARVLALAGLDAGFVLTVVFLVVWLNFVLAIFNLLPIPPLDGYNFALALLPP
                     RFAFSLQRYAPYGVMILLLLVLLPGSPLQLLLRLADPLARAVIGA"
     gene            2569..3054
                     /locus_tag="VFV59_08380"
     CDS             2569..3054
                     /locus_tag="VFV59_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325887.1"
                     /translation="MHRVGQFVAHVRARVTPEEERWVGSILPPPAVALFAAMPVADRR
                     HALDVADRLLAAGRDDRDLLAAALLHDAAKGHRMRLWHRVAGVLLEAFAPTVLRRVAS
                     PDPRSWRHPFHLYLHHGSMSAEMAERAGCSPRAATFIRGEGAGDDAQLQRALEAADDA
                     S"
     gene            3041..3829
                     /locus_tag="VFV59_08385"
     CDS             3041..3829
                     /locus_tag="VFV59_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU5325888.1"
                     /translation="MTQADRMEPIPIEAAATQPMSVGAPEFTVALPVFEGPLQLLLHL
                     IESRQLDVLTVPLADLADAYVEHLARHPVDAANLSDFVATASQLIYLKSRRMLPADPL
                     PPIPDGTDEPDEEELRRRIVEYRLLRDAAVALGERDGVAPVMRREPRESDLPEAPVEP
                     LPIAVLVDALEALAAIPEPVAPPPEVVPREITIGQQIEVLREALSRGGRVILQSILAR
                     CRSRTEAAVTFLATLELVRRREARAEQHEIFGPIVVDALPEAGP"
     gene            3844..4416
                     /gene="scpB"
                     /locus_tag="VFV59_08390"
     CDS             3844..4416
                     /gene="scpB"
                     /locus_tag="VFV59_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5325889.1"
                     /translation="MTDQLDVFYEALLFIAERPLTTAELAELGGVPRLQAEAELAALA
                     ERLEDDARGLRVQRHDDAWQLVTAPEVGARLAAYAAREEARLSPAALEALAVIAYRQP
                     CTRGDVERVRGVDSDYVIRSLLHRRLVVEVGRRDTPGRPVLFGTTFTFLERFGLTSLD
                     DLPPLSSDAAQLAALADVAGRSDASVADGG"
     gene            4406..>5027
                     /locus_tag="VFV59_08395"
     CDS             4406..>5027
                     /locus_tag="VFV59_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU5325890.1"
                     /translation="MAAERLQKLIARAGIASRRAAEQLIADGRVTVNGMRAGLGDSAD
                     AELDDIRLDGRPIGEAPPPLHLAVHKPRGFLSSARDERGRRSVVALVDAGGQRLWPAG
                     RLDVESEGLMVLTNDGEWANRVLHPRYGNEREYAALVNPAPSRRTLQRLLDGVELEDG
                     PARLLSARFAAPPPEVERDRTEDGTWVRVRIGEGRKREVRRLFDAVD"
BASE COUNT          658 a         1863 c         1769 g          737 t
ORIGIN      
        1 gccggggtgc gcgaacgcgt gacgccgcac acgtttcggc acagcttcgc gacccatctg
       61 ctggagggtg gcgccgacct gcgggtggtc caggcgctgc tcggccatgc tagcatcagc
      121 accacccagc tctacacgca cctgaccggc gagcgcgtcc gcgaggtcta cgcgcgggcg
      181 cacccccgag cctgaaagcc cgagcctgaa gcggagcgcc gaacgccgat gagttatgcg
      241 aagaacctcc tgtcgcgtgg cgaggaggtc gtgtacgaga gccggcagca ctggttcgcc
      301 gtcatcgccc gcacctgggc ctggatcatc ctcggcatct tcgcgctggc gctgctgatc
      361 ttcgtggtgt cgggctcgca gccgatcgcc gacaacgaga ccctcgacgg ggttctgacc
      421 atcgtggcgg tcatcggcct gctgctcgcc gtcggattcg tgggcttcgt cctgtgggac
      481 tggcgcaacc aggagtggct catcaccacc cgtcgcgtca tgcgcgccga gggtgtcctg
      541 aacaagtcgg tgaccgatac gagcctcgag aagatcaacg acgcgcggct ggatcagtcg
      601 gtcttcggcc gcatcttcgg gtacggcacg ctcgacatcc tcaccgccgc cgaggagttc
      661 aacggcggca acgtcgccga cttcccgatg atcgcggacc cggtgcagtt caagaaggcg
      721 atgttcgatc agaagcagat gctcgagcag cccgacctcg ccccgccgcg ctatcagcgc
      781 cccgcgccgg tgccgcagat ggagcgcccc gagccgatgc ctccgcgcgc gggttccgat
      841 cgggtggccg tgcgcggcga cgaggcgcgc gagcccgtga ccgcggccgc catggccccg
      901 gagcccgaac acgcggcgct gcccacgccc gcaggtgcac cggccgccca tcgcagcagc
      961 tccgaggagc tcagcgccac gctggagcgc ctggccggcc tgcgcgacaa gggcctgatc
     1021 acgcccgagg agtacgaggc caagaagcgc gacatcctgg agcggatgta ggtggcggac
     1081 gcaccgctcg gcgcgctgcc ggacggactg tacggagagg agctcgaccg gcggatctcg
     1141 gaggcgaccg gcgtcatcgc gggacgcctg tcgacggagc cggggctcga acggccacgg
     1201 gccgtggccg tgctgggatc cggcctgggc ggcgtcgtcg acctgctcga cgcccagcct
     1261 cggctggtga tgtcctacgc ggagatcccc aacgtcccgg gtgccggggt cgagggccac
     1321 gccggccagc tcatcgcggg ccacgtcggc ggcatgccga tggtcgtcct gtcgggacgg
     1381 gcccacccgt acgaggggca cacgcagcgc gcggcgacgc tgctgctgcg ctcggtgctc
     1441 gcgctcggcc cggagctggt gatcctcacc aacgcggccg gcgggctgaa cccggacttc
     1501 gaccccggcg acgtgatgct catcagcgac cacatcaacc tgtccggcga gaacccgctg
     1561 ctcggcccga acctggaccg gttcggtccg cgcttcccgc ccatgacgga cgcctacgac
     1621 ccgcgcctgc gcgcccacgc cctcgagtcg gcggagcgca cccgcatcgg cctccgccag
     1681 ggcgtctaca tcatgctgtc cggaccgtcc tacgagacgc gtgccgagat gcggatgctg
     1741 cggaccctcg gcggggatgc ggtcggcatg agcaccgcgc acgaggtcat cgtggcacgt
     1801 cacgccggcc ggcgggtcct gggcttcagc ctcatcacca acaaggccac cgatgacgtg
     1861 gagaccggcg cgacgcacga ggaggtcatc gaggtcgggc gcatcggcgc cgaacggctc
     1921 gtgaccctgc tcggcgacct gctgcctcgc ctcgcctgat gctgttcagc aaccagccgc
     1981 tgccgtacct cgtggcggtg gcgatcgccc tgctcgtcgg cctcaccttc cacgagttca
     2041 gccatgcggc caccgccgac agcctcggcg accgccgtcc gcgggcgatg ggccgcctca
     2101 cgctgaaccc gctccgccac ctggatccca tcggcgccgc catgctgctg ctcgtcggct
     2161 tcggatgggc gaagccggtc atggtgaacc cttacgcgct gcgcaacgga cggcagggga
     2221 tggccttcgt cgccgcggcg ggaccgctgt cgaacgtcgt cgtcgcgctg ctcttcggcg
     2281 tgatcgcccg ggtgctcgcg ctcgccggcc tcgacgccgg gttcgtgttg acggtcgtct
     2341 tcctggtcgt ctggctgaac ttcgtgctgg ccatcttcaa cctgctgccg atccctccgc
     2401 tcgacggcta caacttcgcg ctggcgctcc tgccaccgcg cttcgccttc tccctgcagc
     2461 gctatgcccc gtacggcgtg atgatcctgc tcctgctcgt cctgctgccc ggatcgccgc
     2521 tgcaactcct cctgcggttg gccgacccgc tggcgagggc cgtcatcggt gcatagggtc
     2581 ggacagttcg tcgcgcacgt gcgcgcccgt gtgacccccg aggaggagcg ctgggtcggc
     2641 tcgatcctgc cgccgcccgc ggtcgcgctg ttcgcggcca tgcccgtcgc cgatcggcgg
     2701 cacgcgctgg acgtcgcgga tcggctgctg gccgcggggc gtgacgaccg cgacctgctg
     2761 gccgccgccc tgctgcacga cgccgccaag ggccaccgca tgcggctgtg gcatcgggtc
     2821 gccggcgtgc tgctcgaggc cttcgcgcca acggtgctgc ggcgcgtggc gagccccgat
     2881 ccgcgatcct ggcgccatcc gttccacctc tacctgcacc acgggtcgat gtcggccgag
     2941 atggccgagc gcgcgggctg ttccccacgc gccgcaacgt tcatccgggg cgaaggcgcc
     3001 ggcgatgacg cgcagctgca gcgagcgctc gaggctgccg atgacgcaag ctgaccggat
     3061 ggagccgatc ccgatcgaag cggccgcgac gcagccgatg agcgtcggcg ccccggagtt
     3121 caccgtggcg ctgcccgtct tcgagggtcc gctccagctg ctgctgcacc tgatcgagtc
     3181 gcgtcagctg gatgtgctga cggtgcccct ggccgacctg gccgatgcgt acgtcgagca
     3241 cctggcgcgt cacccggtcg acgccgccaa cctgtcggac ttcgtcgcca ccgcctcgca
     3301 gctgatctac ctcaagtccc gacggatgct gcccgccgat ccgctgccgc cgatccccga
     3361 cgggaccgat gagccggacg aggaggagct gcgacggcgc atcgtggagt accgcctcct
     3421 gcgcgacgca gcggtggcgc tgggcgagcg cgacggcgtg gcgcccgtca tgcgtcgcga
     3481 gccgcgcgag tcggacctgc ccgaggcgcc ggtcgagcca ctgccgatcg ccgtgctggt
     3541 cgacgcgctg gaagccctgg ccgccatccc ggagccggtc gcaccaccac ccgaagtggt
     3601 gccgcgcgag atcaccatcg gacagcagat cgaggtcctg cgcgaggcgc tgtcgcgcgg
     3661 cgggcgcgtg attctgcagt ccatcctggc ccgctgccgc tcccggacgg aagcggccgt
     3721 cacgttcctc gccaccctgg agctggtgcg ccggcgcgag gcgcgcgccg agcagcacga
     3781 gatcttcggg ccgatcgtgg tggatgccct gcccgaggcc ggcccgtgag cacggaggcc
     3841 gccatgacgg accagctcga cgtcttctac gaggcgctgc tgttcatcgc tgagcggccg
     3901 ctgaccacgg ccgagctggc ggagctgggc ggtgtcccgc ggcttcaggc cgaggccgag
     3961 ctggcggccc tggccgagcg cctcgaggac gatgcccgtg gcctgcgcgt gcagcgccac
     4021 gacgatgcat ggcagctcgt caccgcgccc gaggtcggtg cgcgcctggc cgcgtacgcc
     4081 gcgcgcgagg aggctcggct gtcaccggct gcgctcgagg cccttgccgt catcgcctac
     4141 cggcagccct gcacgcgcgg cgacgtggag cgggtccgtg gcgtcgactc cgactacgtc
     4201 atccgctcgc tgctgcaccg gcgcctggtg gtcgaggtcg ggcgccgcga tacgccgggc
     4261 cgccccgttt tgttcggcac gaccttcacc ttcctcgagc gcttcgggct cacctcgctc
     4321 gacgaccttc ctccgctgtc cagcgacgcc gcgcagctgg ccgcgctggc cgacgtcgcc
     4381 gggcgctcgg acgcctccgt cgccgatggc ggctgagcgg ctccagaagc tcatcgcccg
     4441 ggccggcatc gcgtcgcgtc gggcggccga gcagctcatc gccgatggcc gcgtcacggt
     4501 caacggcatg cgcgccggac tgggcgactc cgcggatgct gagctggacg acatccggct
     4561 cgacggccgc ccgatcggcg aggcaccccc gccgctccac ctggccgtgc acaagccccg
     4621 gggcttcctc tcgtcggccc gcgacgagcg gggccgtcga tccgtcgttg ccctggtgga
     4681 cgcgggcggc cagcgcctct ggccggccgg ccgcctggac gtcgagtcgg aggggctgat
     4741 ggtcctgacc aacgacggcg agtgggccaa ccgcgtcctc cacccgcggt acggcaacga
     4801 gcgggagtac gcggcgctgg taaacccggc gcccagccgc cgaacgctgc agcgcctgct
     4861 cgacggtgtg gagctcgagg acggccccgc ccggctcctg tcggcccgct tcgccgctcc
     4921 gccgccggag gtcgagcgcg accgcaccga ggacgggacc tgggtgcgcg tccgcatcgg
     4981 ggaggggcgc aagcgcgagg tgcggcggct gttcgacgcg gtggacg
//
LOCUS       DASTYP010000189        13873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_272020, whole genome shotgun sequence.
ACCESSION   DASTYP010000189 DASTYP010000000
VERSION     DASTYP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13873
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272020"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..491
                     /locus_tag="VFV59_08400"
     CDS             276..491
                     /locus_tag="VFV59_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325891.1"
                     /translation="MPPMRSLQTPATVLGLVLVLAGVVWIAQGLNLPFAPRSFMTSDR
                     SWIVIGAVVALVGTALVWWGRSARARP"
     gene            complement(488..1078)
                     /locus_tag="VFV59_08405"
     CDS             complement(488..1078)
                     /locus_tag="VFV59_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5325892.1"
                     /translation="MTRYDSRTALVVVDLQNDFADPAGSLSVAGGDAVVPRVNEEVRA
                     ALEAGAAVVATQDWHPESTPHFARDGGIWPVHCVAGTWGAELAPGFRLPADAPRVRKG
                     SNGEDGYSGFTMRDPVSGEEMATELETLLREQGIERVVVCGLATDYCVKATALDAVRL
                     GFEVELLGSAVAAVNLEPDDGERALAEMADAGVAIS"
     gene            1111..2568
                     /locus_tag="VFV59_08410"
     CDS             1111..2568
                     /locus_tag="VFV59_08410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5325893.1"
                     /translation="MDDRLLTDADRALRPGRGERSPWLREALADEGDPPPAAPLHGPT
                     DADVVIVGGGYTGLWTAWHLLERDPALRVVVLEADICGGGPSGRNGGFVNSWWDEASG
                     FVELFGAEGARACLDESAASVRAIGAWCTANGVDAHYRRNGMLVVSTTPFHDGRWEED
                     VEVAARLGEPEAYRALTADDIAARCRSPRFRGGALMRDGATVHPARLVRGLRRRCLER
                     GVAIHEGSPVTAVRRSGGGVVVRTAGGEVRARQAVLAVNAWAAGWPASGSRVLAWGSY
                     MVRSEPIPDLIAAELGWTGGESIVDARFSVHYLHVTRDGRIAIGAGGGRPGFAGRIGP
                     SFTDDVSSARRAAFAFRWLFPQLHEVRLTDAWGGPIDVSPDHLPHAWSTMGGRMHVAA
                     GYSGNGVAPSHLMGRVLAAMALESDEALTRLPIVGRPTPPFPPEPFRYLGARAMREAM
                     IRREDAEEAGRRPGWWWRQLSRLPRRLGYHLGPGA"
     gene            complement(2602..4569)
                     /locus_tag="VFV59_08415"
     CDS             complement(2602..4569)
                     /locus_tag="VFV59_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5325894.1"
                     /translation="MDGYSVTEAASILGVPTEKVWELIARGVLAGAQDGERGMRVFLQ
                     GRPQPPTIDAVRSSEPPPAAREPDRELSPFRELLTEFRNLTERYGQALLALGEARGEV
                     AALRSRVDLLEARMDLRLPPPQATAAPLPEARWVTPIPEPQPEPEPAAPPPVEPVPSM
                     PDAAPATAVEPEPPAPSAEPDVDDAVVAEEPASIGPVVEPQEDEEVLIETASLATAVQ
                     IEADVETIADEAASGGEADAEVETDAEPGTEADQAPSRRRTLRWTTETFAEALARAED
                     PTQPELPGTEEAAAALAALRDDIAVTDAELPRDTAVAEPMEGVEEAAVEEPAAAPELA
                     PEMTAPAADERVTEAAPEPAAQAEVSPEVPAEPEPAPEPAPEPEPEAMRAEPELSIAS
                     EAELAPAASVEAEPEPAEPEPAHEEPERAEEVSAGEPEASLPEPEPLDGAWDRDRYTT
                     AIEEPDWYAEIERPFEEEPAAAAPERESGAEPLPQPGWEEAAPVTAAAETPEAGAEPT
                     PEAPPEPEPAWPEATTEAVAPRVPSDEEELLWFGARAAELAERAGRPAEPEWPPEDGA
                     AEMEVAGRRASAESPFPGSPELRDALSALDALARESRPAEQPIQPGEPQRPPSPWRAS
                     LERPPTAPPQAATPSGPASRAYRRLRRIFPG"
     gene            complement(4601..5884)
                     /locus_tag="VFV59_08420"
     CDS             complement(4601..5884)
                     /locus_tag="VFV59_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEU5325895.1"
                     /translation="MTTMREQTGLDVAALRDHFPSLGRTLDGRPVAYLDGPGGTQVPR
                     SCIQAISAYLERSNANHGGAFAASVETDALLDEVHAAGADFLGAESGDEIVFGPNMTT
                     LTFAMSRALGRELRPGDEIVVTRLDHDANVAPWLAVAEDRGASVRWLELGDDGATLDL
                     DGLERVITDRTRVVAIGLASNALGTVTDVARVIRAAHAVGALAYVDAVHAAPHLPIDV
                     RQLDADVLVTSPYKFFGPHSGMLYGRRELLERLTAYRVRPAGDALPGKWETGTANHEA
                     LAGLLGTFAYLEEVGAAYGGAAHATDRHTRLHAAMTTIRDHERGLSAAVLERLAGVPG
                     LRLYGITDPARLDERVPTFSFTLAGHSPQAVAAHLASRAISVWDGDFYAWEVVRALGL
                     AGSGGLVRIGLVHYNTLQEVDRLVEALHELVGHGR"
     gene            5926..8082
                     /gene="glgP"
                     /locus_tag="VFV59_08425"
     CDS             5926..8082
                     /gene="glgP"
                     /locus_tag="VFV59_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU5325896.1"
                     /translation="MTTSLHVPTLPGHEFSLPAGLEGLGELAYNLWWSWSPHAQALFS
                     HIDHPTWARHRNPIPVLRDTDQARWAELTADEDFMVDASRVLDEFRRYLANGADSWYR
                     SDASRQLSGPIGYFCAEYGLHESMQIYSGGLGILAGDHCKSASDAALPFVAVGLLYRR
                     GYFRQQIDADGHQEHAQPDLDPSQLPLRRARARNGAPLEVSVEIGDRTVHAAVWVAQV
                     GRVPILLLDTDIPRNDPADRPITHILYVRGREMRLCQELILGVGGVRALRALDIDPAV
                     WHLNEGHSAFLLVERARELLVREPGLAAGEALRRVGGSSVFTIHTPVPAGNEVFDRTL
                     VARALRGWFGPARIEPSELLELGRGHTDDPDAPFDMTAFVLRHAVGANAVSQLHGATA
                     TDTWQSLAGRPIDAITNGVHVPTWMGRPVRRLVQRAIGVPLSVDMNGPAPLGSLVDLD
                     DEELWAAHQQQKREMIGFLEGRLSRQFARHGESPDALRAVRHVLDPDSLIIGFARRFA
                     TYKRADLLFRDEERLAAILSSTERPVQVVIAGKAHPADRPGQQVIQHIFALSRSERLR
                     GRVFIVEDYDMRIARFLVGGVDIWLNNPRRPMEASGTSGMKAAINGVPSVSILDGWWD
                     EGFNGRNGWAIGDRTPDGDDQAQDDADAGELYRLLEDEIVPRFFERDASGLPGGWLET
                     MRASVGSALWQFSTARMLTEYVDRLYLPAVREAVAA"
     gene            complement(8086..9519)
                     /locus_tag="VFV59_08430"
     CDS             complement(8086..9519)
                     /locus_tag="VFV59_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5325897.1"
                     /translation="MRRRSILVVTLCALLTTGWTVLTTQPGSHALAADPEAQLAQTRA
                     QLNDARSAQQSLEATLQRQRSELAQLEARSADLGTKLDAAKAELAAVTAEYERVSGLL
                     EQVRQQVADIEARIAELKAQIDELDAQLIQVAVDINRRTADLEAREALLQEHLRAAYE
                     HSQTSLLEVILSADSLDEATTQVGYLMTVSEQDTALADEIRAIRAELETRRATLKDGR
                     RALADARAVARDEEASLKARRDELAALEAQLAELRTAADQKRVEQENALNAALNAQGD
                     VARKIAENEKAAQAAANLAAQLQQQAAAQEAAIAEAKRRAAEEAARRAAAEAAARQNA
                     MSSYGFRWPERSFRVTQEWGPTTFALEPPYTYRGTYYPHFHAGIDFANGCGTPIYSVG
                     PGVVVASGQPLWPWDSGYGVVVDHGGGIQSWYWHMQARVIVSPGTIVTSESLIGYEGT
                     TGMSTGCHVHFAINHGGVWENPRNYLP"
     gene            complement(9673..10713)
                     /locus_tag="VFV59_08435"
     CDS             complement(9673..10713)
                     /locus_tag="VFV59_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5325898.1"
                     /translation="MVDRFKRLLADVDAWSLEHRLPRITRRAVTGFMRHEALQYAGSM
                     AYFAVLSLFQLFVLAIVVGSFFLGQGEARDFVIEQVQAGSPLDADTIGGVIDAAIESR
                     GSIGVIGFAFLLWSALGIFSALSNGIGRAFENAPPRPFLKDKLIGLALMGITGLLALA
                     SLVIGIVTGILQRAATDVLGNIPGGGTAVWLIGLLVPLFLIFLAFWVIYRVVPNRPVG
                     WGEVLPGAVVAALLWTLLRFGFTWYATSVADYDSAFGPISTGITLLVFLYFASVIVLL
                     GAEFARASAVDDEVGRIATADPRFLPVPVEVAPPVPQPKSRRVPRWALLGGVALLGAV
                     VGRLTKRDEEYL"
     gene            10802..12193
                     /locus_tag="VFV59_08440"
     CDS             10802..12193
                     /locus_tag="VFV59_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEU5325899.1"
                     /translation="MARTDTEILHLDGHEVKITNPGKLFFREAGITKLDLVRYYLAVG
                     PGALLGVNDRPMALKRFVDGAEGDPFFQKRAPKSVPAFVRTVELSFPSGRTADEVVVD
                     NLAALAWVVNLGCIDLNPHPTRSGDLEHPDELRVDLDPGPGVAWADVRAVALVVREVL
                     EELGCTGWPKTSGSRGIHVYVRIEPRWGFSDVRRAALALAREVERRAPSLATSKWWKE
                     ERHGVFLDYNQNAKDRTVASVYSVRPRPSATVSAPLRWDEVPDADLEDFTLRTVPARY
                     ERLGDVGAGIDAVAYSLDPLLELVQRHEAEGLGDAPWPPHYAKAAGEPPRVQPSKRRM
                     PDPSELAPTDPTAPRGRVGSVAPALPSPPARNADGTVSPTGRRRSRHPLLVISQAAAK
                     ADAMAGLDRWRDRHREAASYVRDPEDILVDAMRGRFKVWWRVRVNLRNVPEPMRPPAE
                     PPDPDYDPWRDGE"
     gene            12420..13106
                     /locus_tag="VFV59_08445"
     CDS             12420..13106
                     /locus_tag="VFV59_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5325900.1"
                     /translation="MNGRLEMSLRGLRRRRRWSQRHVGHQLGISQQWVSRLEADTRNA
                     RIGLLDRWVNLLGGYLAVEVRVNGERALADAAHATLENQLVADLRRAGWLVEAEVSFN
                     HYGDRGRVDVLAYHAPSRILLVVEVKTRILDLQDLLGRLDVKQRQAPKLARDRGWNVA
                     ASVPAIVVAEGRTSRRRVEQHDALFAPYALRARAARVWLRSPTLPAPSGIFLFEPPRT
                     TTRPSTPWRR"
     gene            complement(13345..>13873)
                     /locus_tag="VFV59_08450"
     CDS             complement(13345..>13873)
                     /locus_tag="VFV59_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5325901.1"
                     /translation="TPGHHFQIAIEMELKGLPAFRTLGARMAGVAYAEGWGLYCERLA
                     DEMGLYASDQERLGMLEAQAFRAARLVVDSGLHAMGWTREQAIAVMHERGSLPMVDAE
                     IEVDRYTVWPGQALSYKLGQREVERARADVAIALGDRFDLRAFHDEVLGHGSLPLATL
                     RREIGGWVEAAVSAG"
BASE COUNT         1816 a         5055 c         4985 g         2017 t
ORIGIN      
        1 gatgcggaga acacgaagtc gatcccgcag tcggaacagg tgatcacctg atcggtgtag
       61 gtcactgacg gacccctccc aacaatgagt tgaaccgcgt cggttccgtc gctcgacgcc
      121 ggccggggat cttgccgctg ccccgggccg acgattggac gtggtggagg ggagggaagg
      181 aagggctggt ggcgccgctc ggtgcggccc gacgggcgga gtatgcgaaa cgcccccgtg
      241 aaccgcaagt gctttttcgc acgtaccatc ggcccatgcc gccgatgcgc tcgctgcaga
      301 ccccggccac cgtcctgggc ctcgtcctgg tcctggcggg cgtcgtctgg atcgcgcagg
      361 ggctgaacct gccgttcgcg ccgcgcagct tcatgacgtc cgatcgatcc tggatcgtga
      421 tcggagccgt cgtcgcgctc gtcggcaccg cgctggtgtg gtggggccgg agcgctcggg
      481 cacgccccta ggagatggcc acgcccgcgt cggccatctc ggccagcgcc cgctccccgt
      541 cgtcgggctc gaggttcacg gcggccaccg ccgagcccag cagctcgacc tcgaagccca
      601 gccggacggc atcgagcgcc gtcgccttga cgcagtagtc ggtcgccagc ccgcagacca
      661 ccacccgctc gattccctgc tcccgcagca gcgtctcgag ctcggtggcc atctcctccc
      721 cggagaccgg gtcgcgcatg gtgaagccgg agtagccgtc ctccccgttg gaccccttgc
      781 ggacgcgggg cgcatcggcc gggagccgga agcccggcgc cagttccgct ccccacgtgc
      841 cggcgacgca gtgcacgggc cagatcccgc cgtccctggc gaagtgcggc gtcgactcgg
      901 ggtgccagtc ctgggtggcg acgacggcgg caccggcctc gagggcggcc cggacctcct
      961 cgttgacgcg cggaacgacc gcgtcgccgc cggcgaccga gaggctcccg gccggatcgg
     1021 cgaagtcgtt ctggagatcc acgaccacca gggccgtgcg cgagtcgtat cgggtcatgt
     1081 cgccgatggt aaccgcgcta tcgtcgcgac gtggacgacc gcctcctgac cgatgccgac
     1141 cgggcgctgc ggcccggtcg gggggagcgc tctccctggc tgcgcgaggc cctcgccgac
     1201 gagggcgatc cgccgccggc agctcccctc cacggaccca ccgatgcgga tgtggtcatc
     1261 gtcggcggcg gctacacagg gctgtggacg gcatggcacc tcctcgagcg cgaccccgcg
     1321 ctgcgcgtgg tggtgctgga ggccgacatc tgcggcggcg gcccgtcggg gcgcaacggc
     1381 ggcttcgtca acagctggtg ggacgaagcg tccggcttcg tcgagctgtt cggggcggag
     1441 ggcgcgcggg cgtgcctgga tgagtcggcg gccagcgtgc gcgccatcgg cgcgtggtgc
     1501 acggccaacg gcgtggacgc tcactaccgc cggaacggga tgctggtcgt gtcgaccacg
     1561 cccttccacg acggccggtg ggaggaggac gtggaggtgg ccgcgcggct cggcgagccg
     1621 gaggcctacc gcgcgctgac ggctgacgac atcgccgcgc gctgccgttc gccgcggttc
     1681 cgcggcgggg cgctgatgcg cgacggcgcc acggtccatc cggcgcgcct ggtccgaggg
     1741 cttcgccgcc ggtgcctcga gcgtggcgtg gccatccacg agggcagccc ggtcaccgcg
     1801 gtccggcgct ccggcggcgg cgtggtggtg cggactgccg gcggcgaggt gcgggcacga
     1861 caggcggtgc tcgcggtcaa tgcgtgggcc gccggatggc cggcgtcagg ttcccgcgtg
     1921 ctcgcctggg gcagctacat ggtccgctcg gagccgatcc ccgacctcat cgccgcggag
     1981 ctgggctgga ccggcggcga gtcgatcgtc gatgcgcggt tcagcgtcca ctacctccac
     2041 gtgacgcgcg acggccgcat cgccatcggt gccggtggcg ggcgccccgg cttcgccggg
     2101 cgcatcggtc cgtccttcac cgatgacgtc tcctccgccc gacgtgccgc cttcgccttc
     2161 cgctggcttt tcccgcagct gcacgaggta cgcctgaccg atgcctgggg cgggccgatc
     2221 gacgtcagcc ccgaccacct gccgcacgcc tggtcgacca tgggcggccg catgcacgtg
     2281 gccgccggct actcggggaa cggggtggcg ccatcacacc tgatgggtcg ggtgctggcc
     2341 gcgatggcgc tcgagtccga tgaggcgctg acgcgccttc ccatcgtcgg acgcccgact
     2401 ccccccttcc cgccggagcc gttccggtac ctgggcgcgc gcgcgatgcg cgaggcgatg
     2461 atccgccggg aggacgcgga ggaggcgggt cggcggccgg ggtggtggtg gcgtcagctc
     2521 agccggctgc ctcgccggct cgggtaccac ctggggcctg gcgcgtaggg tgcaggcgcg
     2581 atgggcgcct gccgacgacg gctatcccgg gaagatgcga cgcagtctgc ggtatgcccg
     2641 ggacgcgggg ccgctcgggg tggcggcctg cggtggcgcc gtgggtggcc gctcgagcga
     2701 tgcgcgccag ggggacggcg gtcgctgcgg ctccccgggc tggatcggct gctctgccgg
     2761 ccgcgactca cgagccagcg cgtcgagggc ggacagcgcg tcgcgcagct cgggtgagcc
     2821 ggggaacggc gactcggccg atgcgcgcct gcccgcgacc tccatctcgg cggctccgtc
     2881 ctccggcggc cactccggct ccgctgggcg gcctgcgcgt tcagccagct ccgccgcacg
     2941 ggccccgaac cagagcagct cctcctcgtc cgacggcacg cggggggcga ccgcttcggt
     3001 cgtggcttcc ggccaggccg gctcgggctc gggcggagcc tcgggggtcg gctctgcgcc
     3061 tgcctcgggc gtctcagcgg cggccgtcac cggtgccgcc tcctcccagc cgggctgggg
     3121 cagcggctcc gcgcccgatt cgcgctcggg tgccgccgcc gcgggctcct cctcgaacgg
     3181 tcgctcgatc tcggcgtacc aatccggctc ctcgatcgcg gtcgtgtatc ggtcccgatc
     3241 ccaggccccg tccaggggct ccggctcggg cagtgacgcc tccggctcgc cggcgctcac
     3301 ctcttcggcc cgctcgggct cctcgtgcgc gggctccggt tcggccggtt cgggttccgc
     3361 ctccacgctc gccgccggcg cgagctcggc ctccgacgct atcgacagct ccggttcggc
     3421 ccgcatcgcc tcgggctctg gctcgggcgc tggctcgggc gctggctcgg gctcggccgg
     3481 cacctccggc gagacctcgg cctgcgccgc cggctcaggc gccgcctcgg tcacccgctc
     3541 gtcggcggcc ggtgccgtca tctcgggggc cagctctgga gcggccgccg gctcctccac
     3601 ggcagcctcc tcgacaccct ccatcggctc ggcgaccgcc gtgtcgcggg gcagctcggc
     3661 gtccgtcacc gcgatgtcgt cgcgcagtgc cgccagcgcg gcggcggcct cctccgtccc
     3721 gggcagctcg ggctgggtcg ggtcttcagc gcgtgccagc gcctcggcga aggtctcggt
     3781 cgtccagcgc agcgtccggc ggcgcgaggg cgcctgatcg gcctccgtgc cgggctcggc
     3841 atcggtttcc acctcggcgt ccgcctcgcc gccagaagcc gcttcgtcgg cgatcgtctc
     3901 cacgtcggcc tcgatctgga cggcggtggc cagggatgcg gtctcgatca gcacctcctc
     3961 gtcctcttgc ggctcgacca ccggaccgat ggacgcgggc tcctctgcca ccacggcgtc
     4021 gtcgacgtcc ggctcggcgg atggcgccgg gggttcaggc tccacagccg tcgctggcgc
     4081 ggcgtccggc atcgacggga ccggctccac cggcggtggc gcggcgggct cgggctccgg
     4141 ctggggctcg ggaatcgggg tcacccagcg ggcctccggg agcggagcgg cggtcgcctg
     4201 cggtggcggc aggcgcaggt ccatgcgcgc ctcgagcagg tccacccggg agcgcagggc
     4261 cgcgacctcg ccgcgggcct cacccagggc aagcagcgcc tggccgtagc gctcggtcag
     4321 gttgcggaac tcggtcagga gctcgcggaa cggtgacagc tcgcgatccg gctcacgagc
     4381 ggcgggcggc ggctccgagg atcgaacggc gtcgatggtc ggcggctgag gacggccctg
     4441 caggaagacg cgcatcccgc gctccccgtc ctgggcaccg gcgagcacgc cacgagcgat
     4501 gagctcccac accttctcgg tcgggacgcc gaggatcgac gccgcctccg tgaccgaata
     4561 gccgtccatc ggccgctagc gtagcgctcc gctcgatcgc tcagcggccg tgaccgacga
     4621 gctcgtggag ggcctcgacc agccggtcga cctcctggag ggtgttgtag tgcaccaggc
     4681 cgatgcgcac caggccgccc gagcccgcca gccccagcgc ccgcaccacc tcccaggcgt
     4741 agaagtcgcc gtcccacacg ctgatcgcgc ggctcgcgag gtgcgcggcc acggcctggg
     4801 gtgagtggcc ggccaacgtg aacgagaagg tcggcacccg ttcgtccagg cgcgccggat
     4861 cggtgatgcc gtagagccgg aggccgggga cgcctgccag ccgctcgagg accgccgccg
     4921 acaggccgcg ctcgtggtcg cggatcgtcg tcatggcggc atgaagccgc gtgtggcgat
     4981 ccgtggcgtg ggcggcaccg ccgtacgccg cgcccacctc ctcgaggtag gcgaaggtgc
     5041 cgagcaggcc ggcgagcgcc tcatggttgg cggtgcccgt ctcccacttc cccggcagcg
     5101 cgtcgcccgc cggccggacg cggtacgccg tgaggcgctc gagcagctcg cggcggccgt
     5161 agagcatccc ggagtgcggg ccgaagaact tgtacgggct ggtgacgagg acgtcggcat
     5221 ccagctggcg cacgtcgatg ggcaggtgcg gcgccgcgtg gacggcgtcg acgtaggcca
     5281 gggcgccgac ggcgtgcgcg gcgcggatga cgcgcgccac gtcggtgacg gtgcccagcg
     5341 cgttcgaggc gagcccgatg gcgacgacgc gggtgcggtc ggtgatcacc cgctcgaggc
     5401 cgtccaggtc gagcgtcgcc ccgtcgtcgc cgagctccag ccaccgcacg ctcgccccgc
     5461 ggtcctcggc gacggcgagc cagggggcga cgttcgcatc gtgatcgagc cgcgtgacga
     5521 cgatctcgtc gccgggtcgg agttcccgcc ctagggcgcg gctcatggcg aaggtgaggg
     5581 tcgtcatgtt cggcccgaag acgatctcgt cgccggactc ggcacccagg aagtcggcac
     5641 cggccgcgtg gacctcgtcg aggagcgcat cggtctcgac cgatgccgcg aaggcgccgc
     5701 cgtggttggc gttcgagcgc tcgaggtagg cgctgatggc ctggatgcag gagcgcggga
     5761 cctgggtgcc gccgggtccg tcgaggtagg cgacgggtcg cccgtcgagc gtgcgcccca
     5821 gcgacgggaa gtggtcgcgc agggcggcga cgtcgaggcc ggtctgctcg cgcatcgtgg
     5881 tcatgcgctg catcctacgc cgggcgcggg ctatcatcgg cgcccgtgac cacgtccctc
     5941 cacgttccca ccctgcccgg gcacgagttc tcgctgcccg ccggcctcga agggctcggg
     6001 gagctcgctt acaacctgtg gtggagctgg agcccgcacg cgcaggcgct cttctcgcac
     6061 atcgaccacc cgacctgggc ccggcaccgc aacccgatcc ccgttctgcg tgacacggac
     6121 caggcgcgct gggccgagct cacggccgac gaggacttca tggtcgacgc cagccgcgtc
     6181 ctcgacgagt tccggcgcta cctggcgaac ggcgccgact cctggtaccg ctccgacgcg
     6241 tcgcgccagc tatccggtcc gatcgggtac ttctgcgccg aatacggact ccacgagtcg
     6301 atgcagatct actccggcgg cctcggcatc ctggccggcg accactgcaa gtcggcctcc
     6361 gatgccgcgc tcccgttcgt cgccgtcggc ctcctctacc gccgcggtta cttccgccag
     6421 cagatcgacg ccgatgggca ccaggagcat gcgcagcccg acctggatcc gtcgcagctg
     6481 ccgctgcgcc gagcccgggc ccgcaacggg gcgccgctcg aggtcagcgt cgagatcggc
     6541 gaccggacgg tgcacgccgc cgtgtgggtg gcgcaggtcg gccgcgtgcc gatcttgctg
     6601 ctcgacaccg acatcccgcg caatgatccc gccgaccgcc cgatcacgca catcctgtac
     6661 gtgcgtggcc gcgagatgcg cctctgccag gagctgatcc tgggcgtcgg cggcgtccgc
     6721 gccctgcgcg cgctcgacat cgaccccgcg gtctggcacc tcaacgaggg gcactcggca
     6781 ttcctgctgg tcgagcgcgc gcgcgagctg ctggttcgcg agcccggcct cgccgcgggg
     6841 gaggcgcttc gacgcgtcgg cgggtcatcg gtcttcacca ttcacacgcc ggtccccgcc
     6901 gggaacgagg tgttcgaccg cacgctggtg gcgcgggcgc tgcgcggctg gttcgggccg
     6961 gcgcgcatcg agccgagcga gctgctggag ctcggtcgcg gccacaccga cgatccggac
     7021 gctccgttcg acatgacggc cttcgtcctg cgtcacgcgg tcggcgccaa cgccgtctcg
     7081 cagcttcacg gcgccacggc gacggacacc tggcaatcac tcgccggccg ccccatcgac
     7141 gccatcacca acggcgtgca cgtccccacc tggatgggcc gcccggtgcg acgcctggtg
     7201 cagcgcgcca tcggcgtgcc actcagcgtc gacatgaacg ggccggcgcc actcggttcg
     7261 ctggtcgacc tcgacgacga ggagctgtgg gccgcccacc agcagcagaa gcgcgagatg
     7321 atcgggttcc tggagggccg gctctcgcgc cagttcgcgc gccacgggga atcgcccgat
     7381 gcgttgcgcg cggtccgcca cgtcctcgat cccgactcgc tgatcatcgg cttcgcgcga
     7441 cgcttcgcga cctacaagcg cgccgacctc ctcttccgcg acgaggagcg cctggccgcc
     7501 atcctgtcgt cgaccgagcg accggtccag gtcgtcatcg ccggcaaggc gcaccccgcg
     7561 gaccggcccg gccagcaggt gatccagcac atcttcgccc tctcccgctc ggagcggctg
     7621 cgcggccggg tgttcatcgt cgaggactac gacatgcgga tcgcgcggtt cctggtgggt
     7681 ggcgtcgaca tctggctcaa caacccgcga cggccgatgg aggcctccgg cacctccggc
     7741 atgaaggcgg ccatcaacgg cgtgccctcg gtctcgatcc tcgacggctg gtgggacgag
     7801 ggcttcaacg gccgcaacgg ctgggccatc ggcgaccgca ccccggacgg cgacgaccag
     7861 gcgcaggacg acgccgatgc gggcgagctg taccgcctgc tcgaggacga gatcgtgccg
     7921 cgcttcttcg agcgggatgc atccggcctc ccgggtggat ggctcgagac gatgcgcgcc
     7981 tccgtcggct ccgccctgtg gcagttcagc acggcgcgga tgctcaccga gtacgtcgac
     8041 cggctttatc tgccggccgt gcgcgaggcc gtcgccgcct agccgctacg gcaggtagtt
     8101 cctcgggttc tcccacacgc cgccgtggtt gatggcgaag tggacgtggc aacccgtcga
     8161 catacccgtc gtgccctcgt agccgatcaa cgactcgctg gtcacgatgg tccccggcga
     8221 gacgatgacc cgcgcctgca tgtgccagta ccagctctgg atgccgccgc cgtggtcgac
     8281 gaccacgccg taccccgagt cccacggcca cagcggctgg ccagaggcca ccacgacgcc
     8341 cggcccgacg gagtagatcg gggtgccgca gccgttggcg aagtcgatgc cggcgtggaa
     8401 gtgcgggtag taggtgccgc ggtaggtgta cggcggctcg agggcgaagg tcgtggggcc
     8461 ccactcctgc gtcacccgga acgagcgctc cggccagcgg aagccatacg acgacatcgc
     8521 gttctggcgc gctgcggcct cggccgcggc acggcgggca gcctcctcgg cggcgcgccg
     8581 cttggcctcg gcgatggcgg cctcctgggc cgccgcctgc tgctggagct gcgcggccag
     8641 gttggcagcg gcctgcgcgg ccttctcgtt ctcggcgatc ttgcgcgcca cgtcgccctg
     8701 cgcgttgagc gcggcgttca gcgcgttctc ctgctcgacc cgcttctgat cggccgcggt
     8761 gcgcagctcg gccagctgcg cctccagcgc ggccagctcg tcgcgacggg ccttgagtga
     8821 cgcctcctcg tcgcgcgcga cggcgcgcgc atcggccagg gctcgacggc cgtccttcag
     8881 ggtcgcccgc cgagtctcga gctcggcccg gatggcgcga atctcatcgg ccagggcggt
     8941 gtcctgctcc gagacggtca tcaggtagcc gacctgggtc gtcgcctcgt cgagggagtc
     9001 ggcggagagg atcacctcga gcagcgaggt ctggctgtgc tcgtaggcgg ctcgcaggtg
     9061 ctcctggagc agcgcctcac gcgcctcgag gtccgcggtg cggcgattga tgtccaccgc
     9121 gacctggatg agctgggcat cgagctcgtc gatctgcgcc ttgagctcgg cgatgcgcgc
     9181 ctcgatgtcg gccacctgct ggcgcacctg ctcgagcagg ccgctgacgc gctcgtactc
     9241 cgcggtcacg gcggcgagct ccgccttggc ggcatccagc ttggtgccga gatcggcact
     9301 ccgagcctcc agctgggcga gctccgagcg ctggcgctgc agggtagcct ccagcgactg
     9361 ctgcgccgac cgtgcgtcgt tcagctgggc gcgggtctgg gccagttggg cctcgggatc
     9421 ggcagcaagg gcatggctgc cgggttgggt tgtgaggacg gtccacccgg tcgtcagcag
     9481 ggcacacagc gtgacgacga ggatggatcg ccgcctcatc gagcggtgat ccgcactatg
     9541 caattgccct tttgccccga tagcggttcg tgttcggggc cgatggtggc cgatgccttc
     9601 cggcaccgga acgcgcccaa ggtactacac cggtccagcc cggtgccacc gttggtcctg
     9661 gtcaggacgc cgctacaggt actcctcgtc gcgcttggtg aggcggccga cgaccgcgcc
     9721 gagcagcgcc acgccgccca gcagcgccca cctcgggacg cgcctggact tcggctgcgg
     9781 caccggcggc gcaacctcga ccggcaccgg caggaagcgc ggatcggccg tggcgattcg
     9841 tccgacctcg tcgtcgaccg cgctggcgcg ggcgaactcg gcgcccagca gcacgatcac
     9901 gctggcgaag tagaggaaca ccagcagcgt gatgcccgtc gagatcggac cgaaggcgct
     9961 gtcgtagtcc gcgacgctgg tcgcgtacca ggtgaacccg aagcgcagca gtgtccacag
    10021 cagcgccgcc acgacggctc ccggcagcac ctcgccccag cccaccgggc ggttcggcac
    10081 gacgcgatag atgacccaga aggcgaggaa gatcaggaac agcgggacca gcagaccgat
    10141 gagccacacg gccgttccgc cacccgggat gttgcccagc acgtcggtcg cggcgcgctg
    10201 caggatgccg gtcacgatgc cgatgaccag cgaggccagc gcaagcagcc cggtgatgcc
    10261 catcagcgcc aggccgatga gcttgtcctt gaggaacggt cgcggggggg cgttctcgaa
    10321 ggcgcggccg atgccattcg acagcgccga gaagatgccg agggcggacc acagcaggaa
    10381 cgcgaagccg atgacgccga tgctgccgcg cgactcgatg gccgcgtcga tgaccccgcc
    10441 gatcgtgtcc gcgtcgaggg gcgagccggc ctgtacctgc tcgatgacga agtcccgtgc
    10501 ctcgccctgg cccaggaaga acgagccgac cacgatggcg agcacgaaca gctggaacag
    10561 cgacagcacg gcgaagtagg ccatgctgcc ggcgtactgg agcgcctcgt gccgcatgaa
    10621 gccggtcacc gcgcgtcgcg tgatgcgcgg caggcggtgc tcgaggctcc acgcgtcgac
    10681 atcggcgagg aggcgcttga atcggtcgac catcggttgc cggtcctccg caaggggcgt
    10741 gccgctgggt cggtggcgcc gggcggtggc attgggtccg tggcgctacc ctgtcgctcc
    10801 tatggcgcgc accgacaccg agatcctgca cctcgacggc cacgaggtga agatcacgaa
    10861 tcccgggaag ctcttcttcc gcgaggccgg gatcaccaag ctcgacctgg ttcgctacta
    10921 cctggccgtc gggcccgggg cgctgctcgg cgtcaacgac cggccgatgg cgctgaagcg
    10981 cttcgtcgac ggtgccgagg gcgacccgtt cttccagaag cgtgccccga agtccgtgcc
    11041 ggccttcgtc cgcacggtcg agctgtcgtt cccctcgggt cgaacggcgg acgaggtggt
    11101 ggtcgacaac ctcgcggcgc tggcgtgggt cgtgaacctg gggtgcatcg atctcaatcc
    11161 gcacccgacg cgttccggcg acctcgagca tcccgacgag ctgcgcgtgg accttgatcc
    11221 cgggcctggc gtggcgtggg ccgacgtgcg cgccgtcgcc ctggtggtgc gcgaggtgct
    11281 cgaggagctc ggctgcaccg gctggcccaa gacgtcgggc tcgcgcggga tccacgtcta
    11341 cgtccgcatc gagccgcgct ggggcttctc cgacgtccgt cgcgccgcgc tggcgctggc
    11401 ccgcgaggtc gagcgccgcg ccccctccct ggcgacctcg aagtggtgga aggaggagcg
    11461 ccacggcgtg ttcctcgact acaaccagaa cgccaaggat cggaccgtgg catcggtcta
    11521 ctccgtgcgg ccccggccgt cggccaccgt ctcggcgcct ttgcgttggg acgaggtgcc
    11581 ggatgccgac ctcgaggact tcaccctgcg caccgtcccg gcacgctacg agcggctggg
    11641 ggatgtcggc gcggggatcg acgccgtcgc gtactcgctc gacccgctgc tcgagctggt
    11701 gcagcgccac gaggccgagg gcctggggga cgcgccgtgg ccgccgcact acgccaaggc
    11761 ggccggggag ccgccgcgcg tccagccctc gaagcgccgc atgcccgatc cctccgagct
    11821 ggcacccacc gatcccaccg cccctcgggg ccgcgtcggc tccgtcgcgc ctgcgcttcc
    11881 ctctcccccg gcacggaacg ccgatggcac cgtctccccc acgggccgtc ggcggtcgcg
    11941 ccatccgctg ctggtcatca gccaggcagc cgccaaggcc gatgcgatgg ccggcctcga
    12001 ccgttggcgc gatcggcacc gggaggctgc ctcgtacgtg cgcgacccgg aggacatcct
    12061 ggtggacgcc atgcgcggcc gcttcaaggt gtggtggcgc gtccgggtca accttcgcaa
    12121 cgtcccggag ccgatgcggc cgcctgcgga accgcccgac cccgactacg acccctggcg
    12181 ggacggcgag taggttcggg tcgccagcca ccgggagcct cgactccgcc attcacgcgt
    12241 ggacggcgtg aacgtcagga ccgaacatcc tgagtccgcc atccgacccg cgttcacgcc
    12301 caccaagcgt gatctggcac ggtcgggatc tccacccacg gccgctacca cggggaagcg
    12361 tgaatggcgg taacccagcc gtaagcgggc agtacacgtc gacacgtcat cctcgaggcg
    12421 tgaacggacg ccttgagatg tcgctgagag ggctgcgccg ccgccgccgt tggtcgcagc
    12481 gccacgtcgg ccatcaactc ggcatctcgc aacagtgggt gagccgactc gaggccgaca
    12541 cgcgcaacgc gcgtatcggt ctcctggacc ggtgggtcaa tctccttggc ggctacctcg
    12601 cggtcgaggt gcgagtcaac ggcgaacgag cgctggccga tgccgcgcac gcgacgctcg
    12661 agaaccagct tgtcgcggat cttcggcggg cggggtggct cgttgaggcc gaggtcagct
    12721 tcaaccacta cggcgatcgg ggtcgggtcg acgttcttgc ctatcacgcg ccctcgcgga
    12781 ttctgctcgt cgtcgaggtc aagacgcgga tcctcgatct acaggatctg ctcggacgtc
    12841 tcgacgtcaa gcagcgacag gcgccgaagc tcgcgcgcga ccgcggctgg aacgttgcgg
    12901 cgagcgtgcc ggcaatcgtc gttgccgagg gcagaacctc tcggcgacgc gtcgagcagc
    12961 acgacgccct cttcgcgccg tatgccctcc gcgcccgggc agcgcgtgtg tggctgcggt
    13021 ccccgacgct gccggcgccg tcaggcatct tcctgttcga gccgccgcga accacgactc
    13081 ggccatctac gccttggagg cgttgaaggc ccctgggcga cgcgtgatct cgcttacctc
    13141 gccctgcggc accactcagc ggcgtgagtg gcagagccag ggccggcgtc cgccgcattg
    13201 accaccacac ggcgtgagtg gcagagccga tcggtgggtt cccgcgccac tcaccacccc
    13261 aggacgtgaa tggcagagtc cactccatgc ctcggccgct ccgctttgcc tggtgcagct
    13321 aggccggcac cgacccggcc ccgactaccc ggcgctgacg gcggcctcca cccagccgcc
    13381 gatctcgcgg cggagcgtgg cgagcggcag cgagccgtgg ccgagcacct cgtcgtggaa
    13441 ggcgcggagg tcgaatcggt cacccagggc gatggcgaca tcggcccgcg ctcgctcgac
    13501 ctcgcgctgg ccgagcttgt aggagagcgc ctggccgggc cagacggtgt atcggtccac
    13561 ctcgatctcg gcgtcgacca tgggcagcga gccgcgctcg tgcatgacgg cgatggcctg
    13621 ctcgcgcgtc cagcccatgg cgtgcaaccc cgagtcgacg accaggcgcg ccgcgcggaa
    13681 cgcctgcgcc tccagcatcc cgaggcgctc ctggtccgag gcatacaacc ccatctcgtc
    13741 ggcgagccgc tcgcagtaca gcccccagcc ctcggcgtag gccacgccgg ccatccgggc
    13801 gccgagggtt cggaacgcgg gcagcccctt cagctccatc tcgatcgcga tctggaagtg
    13861 gtgcccgggc gtc
//
LOCUS       DASTYP010000190        13945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1073002, whole genome shotgun sequence.
ACCESSION   DASTYP010000190 DASTYP010000000
VERSION     DASTYP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073002"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..102
                     /locus_tag="VFV59_08455"
     tRNA            18..102
                     /locus_tag="VFV59_08455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Leu,seq:gag)
     gene            380..456
                     /locus_tag="VFV59_08460"
     tRNA            380..456
                     /locus_tag="VFV59_08460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:414..416,aa:Val,seq:tac)
     gene            587..1165
                     /locus_tag="VFV59_08465"
     CDS             587..1165
                     /locus_tag="VFV59_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5325902.1"
                     /translation="MPLWGWILLAAIVIVVLFVIFTYNGLVRLRNMVDEAWNQISVQL
                     KRRHDLIPNLVNAVKGYMEFEQETLTRVIEARNAAVGATDRGPAAAGEAGAAENVLTG
                     ALRQLFALMENYPQLKANENVMQLQEELTTTENQIAFSRQHYNATVREFNTSIQTFPN
                     NTIAGMFGFRERDYFQIAESDTAVPQVDLQLS"
     gene            1185..2153
                     /locus_tag="VFV59_08470"
     CDS             1185..2153
                     /locus_tag="VFV59_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5325903.1"
                     /translation="MTAEGRPLATTFYREIDRNRRRSWLLVVAVAIVLGVLGGVIGFA
                     TGIDWFGVVLAVAVAAVMTVGSYFGGDALVMATSGAREIETAQPAEKYRQLVNVVEEM
                     RLAGGLPRPRVWVIDDSAPNAFATGRDAEHASIAVTAGLLDKLDREQLQGVIGHEMSH
                     IGNLDIRFTLLVGVLVGSIALLADWFLRFTFWGGGRRGGNDRGGGGGGGAALIFVLAL
                     VLAVLAPMIARIIQAAVSRSRESLADASAVELTRNPVALARALRTIADDREVLEVANR
                     ATQHLYIVNPIKSFEERAKSLWDTHPPIGERIRALEAMGAQAGIGG"
     gene            2263..2338
                     /locus_tag="VFV59_08475"
     tRNA            2263..2338
                     /locus_tag="VFV59_08475"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2296..2298,aa:Phe,seq:gaa)
     gene            2370..2445
                     /locus_tag="VFV59_08480"
     tRNA            2370..2445
                     /locus_tag="VFV59_08480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2403..2405,aa:Gly,seq:gcc)
     gene            2516..2589
                     /locus_tag="VFV59_08485"
     tRNA            2516..2589
                     /locus_tag="VFV59_08485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2548..2550,aa:Gln,seq:ttg)
     gene            2606..2680
                     /locus_tag="VFV59_08490"
     tRNA            2606..2680
                     /locus_tag="VFV59_08490"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2639..2641,aa:Cys,seq:gca)
     gene            complement(2746..2958)
                     /locus_tag="VFV59_08495"
     CDS             complement(2746..2958)
                     /locus_tag="VFV59_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325904.1"
                     /translation="MSPVALTLILCLFLAAAGFIGLVVGRTLRSGRGDLDAAGWVIVL
                     ALGFAAAAIVTLELVLPRLEALLLHR"
     gene            3307..4848
                     /locus_tag="VFV59_08500"
     CDS             3307..4848
                     /locus_tag="VFV59_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5325905.1"
                     /translation="MLLAACGGQAPAVSTPASTAPSASAAASDSASGAVSSLDAVKTA
                     TIQIEAEGTFQDPEVGTVYNAAGRGSGFIIDPSGIAVTNNHVVTGSALLKVWVGGESE
                     PRNAKILGVSECSDLAVIDIDGDGYPFLDWYDGSVSTGLDVYAAGFPLGDPEFTLTRG
                     IVSKENADGETSWASVDEVVEHDATINPGNSGGPLVTADGQVVGVNYAGASETNQYYA
                     INGSAAQDIVAQLEAGSDVQSIGVNGQAVNDGETISGIWVSSVKSGSPADAAGVQGGD
                     IITTMEGLVLSTDGTMADYCDILRSHDAEDTLAIEVLRFASEEVLEGQLNGRALETSF
                     SFAQELGDEVDENGGGGGSTGYSDFVEVQDDSGAIGMVVPAEWAEVDGSAWIAGESNV
                     GPAIIATTSMDGWNNGWDVPGIFFGASSSLRSSYDVQGMLDANDFSDTCTYESRSDYE
                     DPLYTGAYDVYRDCGGSSNTFVVLAAEPEDGSFIMLLQVVLVSEADAQALDQILNTFQ
                     VIGSL"
     gene            complement(4923..7424)
                     /locus_tag="VFV59_08505"
     CDS             complement(4923..7424)
                     /locus_tag="VFV59_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEU5325906.1"
                     /translation="MPHRGEAGRRRARALLILVVLIASAVPVAPARASEATIERTYGA
                     DRYATAAAVSEDFAPGLPAVFVATGQNFPDSLAGGPAAARLGAPILLATASRLPDATR
                     SALSRLQPRSIVVLGGPGVVSDAVAAALGTYAPTSRLFGPTRYETAVAISRTFPAGVP
                     VAYVATGRTFPDALAAGAAAAAQGGPLLLVDTASIPDAVRAELARLQPQRIVVAGGAA
                     VVSDGVLAQLNAMAPGGATRLWGADRFGTAAAISRAAFASAPSAHIATGLGFADAVAA
                     VPAAARTRGPLLLSTTAGLPSATTAEIARLRPNHTVVLGGPTVLSKAVIDQAVAASGL
                     TLIGPHAFRWDAFSLETVPDAYLAYNGDTIVAPYGQLENGVYKWNYNGTWVDHPVGQA
                     QYVVNMLRNYRLDPKQEYLDLAIANASRLLERAVRHEGAVFFPYGFDFYLHGRGTMRA
                     PWYSGMAQGIALSGFVRLYQITHDRRWLQAANETYASFLVGRDSGGPWVTSTDNGLLW
                     FELYPWTPNDHTYNGHNWAIYGLYDYWRLTGSSQAQQLIMAGLTTSKRALSIVRVPGG
                     VMRYCIAQSCLDRNVVNSDYHLVVTNQMIQLFRYTRDEDFARMADAFVTDTPYFKDSG
                     TVVLMPGTHVGASFDSAGMESAAANTVITEPTEVAYAQRNVPYGWVRPGNGVRMYLTE
                     GPLAGTWVRETPMAFVRGFVDRYDFYWGRNFAVPAGDVTGYRFNDDGTIAETLTAPSS
                     SSAWRYSSSARINGQRAGLVVDGPLAGYWVALDGPWTAEATSVTAAAATAQGGASASG
                     MDTQRSAATPSVAPPPLPPSDPLIPPPEPGQLDAP"
     gene            7556..7726
                     /locus_tag="VFV59_08510"
     CDS             7556..7726
                     /locus_tag="VFV59_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325907.1"
                     /translation="MTHRDDDRPAPDSLRGKLDDLFDEDEDTSSEETESSLDDEAIAD
                     GSLPKRPTDPPF"
     gene            7785..7868
                     /locus_tag="VFV59_08515"
     tRNA            7785..7868
                     /locus_tag="VFV59_08515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7818..7820,aa:Leu,seq:taa)
     gene            complement(7873..8052)
                     /locus_tag="VFV59_08520"
     CDS             complement(7873..8052)
                     /locus_tag="VFV59_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325908.1"
                     /translation="MTEEYERHAGKAPEDRHPAQLGSETDPSDYADPRVAQPGEDPPG
                     EDAMEPIVDGERRPA"
     gene            complement(8085..9314)
                     /locus_tag="VFV59_08525"
     CDS             complement(8085..9314)
                     /locus_tag="VFV59_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325909.1"
                     /translation="MPSNPILIVARGSELRTELETALGGAGYSTTSVSTPADAVAAMR
                     GSPPDLVVAEGLAASGAIGSLRTATGSGVTPVLVVAPAHDVEARIAFLEAGADDVVPA
                     SFGRNELEARVMALLIRAGRVRPEVSARGTTAELVAFFSPKGGVGTTTLAVNVAVLLA
                     GGAPLPQAAAATGAPTLPASRVLLIDLDLQFGQVATHLNVTPRYDIAGLASDEQALAD
                     PDLARSYLTTHESGLAVLAAPARPEADFRVTLDQLQRIVEVLRPGFDQIVVDLGSRLD
                     PRALWVLEQSDAHVFVLFPEIAALRSMSLLLAFLAETTPLQARTHIVVNHVFPKELLK
                     TRDVENLLRTKPAAEIPYTEVEMIRSVNEGVPLVMGRPASPASLALRRVAQAVIGVDV
                     APAGQPRQQRRGLFGRR"
     gene            9519..10025
                     /gene="rpsF"
                     /locus_tag="VFV59_08530"
     CDS             9519..10025
                     /gene="rpsF"
                     /locus_tag="VFV59_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5325910.1"
                     /translation="MRRYELMLLLRPDLEDDALQSAVEKVTRAIVNAGGSLTKVSPWG
                     KRRLAYDIKHFRDASYFLIHFDIEPAAIRELERGMLISEDILRHLVTVIEDRGPAEEL
                     APAAAPRGATGGAGDDEVEEAEEVEDADDETSDDTSDDTSEDQEAPAEDAADTDAVPT
                     SDEERGEG"
     gene            10053..10535
                     /gene="ssb"
                     /locus_tag="VFV59_08535"
     CDS             10053..10535
                     /gene="ssb"
                     /locus_tag="VFV59_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5325911.1"
                     /translation="MTLNKMMIIGNLGADPELRYTPSGKAVTDLRVAVNNNQRGPDGE
                     WVEETLWFRVSVWEQQAERLAEQLRKGNKVYAEGQLRVREFEGRDGEKRQSLELAFAR
                     VVNLERRSREDGEGGSYGGGGGGGGSYGDAPPSVRPRGAQQRPGSSPGDEMDVDDIPF
                     "
     gene            10555..10824
                     /gene="rpsR"
                     /locus_tag="VFV59_08540"
     CDS             10555..10824
                     /gene="rpsR"
                     /locus_tag="VFV59_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU5325912.1"
                     /translation="MASPRSRRETADYYRDRRRRKVCNFCVDKAESIDYKEVNRLRRY
                     LSERAKIEPRRKTGTCARHQRMLTTALKRARQVALLPYAPQHLRP"
     gene            10876..11682
                     /locus_tag="VFV59_08545"
     CDS             10876..11682
                     /locus_tag="VFV59_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5325913.1"
                     /translation="MLTALLLFLGGLGAGLFGSLLGLGGGILLVPLLTVGFGYSIVSA
                     VGTSLVCVVATSAGAASRYVRTGQADVRLGITLEVATATGALAGGFIAGVIPERLLAG
                     FFALLLLYTAYTLGRRALDPAPTGTAAEARSWARTGEEAPAYRTTRLPAAMAGSLGAG
                     VISALLGIGGGIVKVPIVHLVMGAPMHVSVATSNFIIGVTAATGAYVYLFRGEISPAV
                     AGPVVLGVALGAAAGARISGRVSGRWLSIIFLAVLLYTAFQMAQRALGAG"
     gene            11679..12077
                     /locus_tag="VFV59_08550"
     CDS             11679..12077
                     /locus_tag="VFV59_08550"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEU5325914.1"
                     /translation="MSAPAAPPDMTRAGPLRRWVATVLTIGVWSSVALVAVGLGWAVV
                     SGRPLEVARGSLLEELTAGEPGSVVLLGILLLVLTPIAQLVAAAAAFARNRERGYVIV
                     TLVVLGVLVFSLVLAALTGGGAESARAAVV"
     gene            12233..13246
                     /locus_tag="VFV59_08555"
     CDS             12233..13246
                     /locus_tag="VFV59_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU5325915.1"
                     /translation="MPELVSWSIFAIAIFGLLALDLFVFHREAKPVSMREASVWVTVW
                     VSLGLAFGAFIFLTRGATSGGEYLAGYLIEYSLSMDNVFVFAVLFAYFAVPAQYQHRL
                     LFWGVLGAIVFRAVFILAGTALLEAFHVVVYVFGALLLFTAWRMVTAGTENVDPNANP
                     FLRLMRRVIPVTEGYQGQRFFVRRDGRLWATPLFAALVVIESSDIMFAIDSVPAILSI
                     TTDTFIVFTSNAFAIMGLRSLYFLLAGLIDRFEYLKYGLAALLAFAGVKMLLSDVIHL
                     DVWVSLGIIVAILGSSIVISLMATRSVGSGAPRGQVRVGSSLTVALVVGALIAASALL
                     WLV"
     gene            13256..>13945
                     /locus_tag="VFV59_08560"
     CDS             13256..>13945
                     /locus_tag="VFV59_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325916.1"
                     /translation="MDVVLLVVALGVILGSAELFTNGIEWFGHKLDLAEGAVGSVLAA
                     VATAMPETLIPTIAIVGPALSGRDPQASHEIGVGAILGAPFMLSTVAMFVTGAAVLAF
                     ARRGRRAEAMDVSTPVLARDVGFFVVGYTVAIGTAFLPAVAWIRWSAAALLLGMYLYY
                     VRLHFADDREEGDASDLNRLHATRLPGIRRRSALEVQFLPDVAEDEGDTTPRLGHEPR
                     MRIVVGQVLAAM"
BASE COUNT         2019 a         4800 c         5014 g         2112 t
ORIGIN      
        1 tcggtaccac cctcgatgcc caggtggcgg aattggtata cgcgtacgtt tgaggggcgt
       61 atggggaaac ccatccgggt tcaagtcccg gcctgggcac caattcagat ctgatccccg
      121 caccgatgcg ccgctcgctc cgcgagcggc gttcgtgtgt tccgcgggcg ttgcggtctc
      181 gaccgtcgcc agtcttccgg attcacacca ggtgagcgtg gggcgcccgg atcgtcgcgg
      241 aatcgtccgg attcacacca gctgaccgct acgcacccgc aaaccggtcc gtcgagcggg
      301 caccgcccgc ttgatgcggg cgtggtgtga atccggcctg tcggagcacg ctggcgggca
      361 tcggctacga tcacgcggag ggcggttagc tcagctggac agagcgcctc gtttacaccg
      421 aggaggtcgg gggttcgagc ccctcaccgc ccaccacgcg tggggctgag cagtgtttcg
      481 ttgctgcgag ccgacgacgc acgcggaagg gggcgggacg gccctggcgc gagcacacgc
      541 ctgctagcat cgtcggtgca aacgcccgag cgcgaggaac acgccgatgc cactctgggg
      601 ctggatcctc ctggcggcga tcgtcatcgt cgtcctcttc gtcatcttca cgtacaacgg
      661 cctggtccgc ctgcggaaca tggtcgacga ggcgtggaac cagatcagcg tgcagctgaa
      721 gcgacggcac gacctgatcc cgaacctcgt caacgccgtg aagggctaca tggagttcga
      781 gcaggagacg ctgacgcgcg tcatcgaggc tcgcaatgcc gcggtgggcg ccaccgatcg
      841 gggtcccgcg gccgccggcg aggctggagc cgccgagaac gtcctgaccg gggccctgcg
      901 ccagctgttc gccctgatgg agaactaccc gcagctgaag gccaacgaga atgtcatgca
      961 gctccaggag gagctgacga cgaccgagaa ccagatcgcc ttcagccggc agcactacaa
     1021 cgcgacggtg cgggagttca acacctccat ccagacgttc cccaacaaca cgatcgcggg
     1081 catgttcggc ttccgcgagc gggactactt ccagatcgcc gagtccgata cggcggtccc
     1141 gcaggtcgac ctgcagctca gctgagccga cctgagctga gccgatgacc gccgaggggc
     1201 ggccgctggc gacgacgttc taccgcgaga tcgatcgcaa ccggcgccgc agctggctgc
     1261 tcgtcgtcgc cgtggcgatc gtcctcggtg tgctcggcgg cgtcatcggc ttcgcgaccg
     1321 gcatcgactg gttcggcgtc gtgctggccg ttgccgtggc ggccgtcatg acggtcggct
     1381 cctacttcgg tggcgatgcg ctggtcatgg cgaccagcgg ggcacgcgag atcgagacgg
     1441 cgcagccggc cgagaagtac cgtcagctgg tcaacgtggt cgaggagatg cggctggccg
     1501 gcgggctccc ccggccgcgg gtctgggtca tcgacgactc cgcgcccaac gcgttcgcga
     1561 ccggacggga cgccgagcac gccagcatcg cggtcaccgc gggcctgctc gacaagctgg
     1621 accgcgagca gctgcagggc gtcatcggcc atgagatgag ccacatcggc aacctggata
     1681 tccgcttcac gctgctggtt ggcgtgctgg tcggcagcat cgcgctgctg gccgactggt
     1741 tcctgcgctt caccttctgg ggtggtggcc gtcgcggtgg caacgaccgc ggcgggggcg
     1801 gcggcggggg agcggcgctc atcttcgtcc tcgcgcttgt ccttgctgtc ctggcgccga
     1861 tgatcgcgcg aatcatccag gccgccgtca gccgcagtcg cgagtcgctg gccgatgcga
     1921 gcgccgtcga gctgacccgc aacccggtgg cgctcgcccg ggcgcttcgg acgatcgcgg
     1981 acgaccgcga ggtgctcgag gtggcgaacc gggcgaccca gcacctgtac atcgtcaacc
     2041 ccatcaagag cttcgaggag cgggcgaagt ccctgtggga cacccacccg cccatcggcg
     2101 agcggatccg ggcgctggag gcgatgggcg cccaggccgg catcggcggg tgaggctccc
     2161 gcggtcgcgg cgcggttgcg agcgcgggaa ggcatcggta tccttgcgtc cgcctgaagc
     2221 ggtaccgcga cgggagctga ggaggcctca gcgacgatcg gcgccgaggt agctcagttg
     2281 gtagagcacg ggactgaaaa tcccggtgtc gccagttcga tcctggccct cggcaccaac
     2341 gacgcggtat cattgagggg aacgagcggg cggaagtggc tcagttggta gagcatcacc
     2401 ttgccaaggt gagggtcgcg ggttcgagtc ccgtcttccg ctccattttc ccgcaccgat
     2461 tggactgggc ggctggttgg ggtcgtcgct gacctgctag cgtgagacga ggcgatgccc
     2521 cgtcgtctag tggtaggaca gcggactttg gatccgtgaa ccgaggttcg aatcctcgcg
     2581 gggcagccag ccgcaataca tcggtggcga cgtgcccgag tggttcaggg agcggtctgc
     2641 aaaaccgcgt acacgggttc gattcccgtc gtcgcctcca caagcctcgg gggaattgat
     2701 tcgggtcgga tcgcaagctc ggagcgatcc ggcccgtctt ccttctcacc gatgcaggag
     2761 cagcgcctcc aggcggggaa gcaccagctc cagcgtcacg atcgcggcag ccgcgaagcc
     2821 cagggccagc acgatcaccc accccgcggc gtccaggtcg ccacgtcccg atcgaagtgt
     2881 tcggcccacc accaagccga tgaagccggc cgcggcgagg aagaggcaga ggatcagcgt
     2941 cagcgcgacg gggctcacgc gcgaatcgta cgcgccgcgc acgggtacca ttgcctgagg
     3001 ggttccgacc cgatatagtc cgcgcgacgc tattacccga gcagaggggg agtgcccgcc
     3061 gtccggcacc atcgcgtgcc gttttctcct cgacgaccca ccgccggcgc cccgtgcccg
     3121 gcttccggcc gacgaacgcc cagacagctg cccgctgtgc ccgcccggac cccacgaccg
     3181 tgcgtcctcg tgcagcctcc tgtgcccaag ggggatctga ttgcggcccg ccccaccgtg
     3241 aacagaaggg acgccagaac ggtcatgttt cgccccaggt ctctttcgtc catcgtgttg
     3301 atgggcatgc tcctggccgc gtgcggcggc caggcgccgg cagtatccac gccggcgagc
     3361 accgcaccat cggcctccgc ggccgccagt gattcggcat cgggcgccgt ctcgagcctc
     3421 gacgccgtca agaccgccac catccagatc gaggctgagg gaaccttcca ggatcccgag
     3481 gtcggcacgg tctacaacgc cgccggtcgt gggtccggct tcatcatcga tccgtccggg
     3541 atcgcggtca ccaacaacca cgtcgtgacg ggctcggccc tgctcaaggt gtgggtcggc
     3601 ggtgagagcg agccgcgcaa cgccaagatc ctcggcgtca gcgagtgctc cgacctcgcc
     3661 gtcatcgaca tcgacggcga cggctaccca ttcctcgact ggtacgacgg gtcggtctcg
     3721 actggcctcg acgtgtacgc cgcgggcttc ccgctggggg accccgagtt caccctgacg
     3781 cgtgggatcg tcagcaagga gaacgcggac ggcgagacgt cgtgggccag cgtcgacgag
     3841 gtggtcgagc acgacgccac catcaacccc ggcaactccg gcggcccgct ggtgacggcg
     3901 gacggccagg tcgtgggcgt caactacgcc ggcgccagtg agaccaacca gtactacgcc
     3961 atcaacggca gcgccgcaca ggacatcgtg gcgcagctgg aggccggctc ggacgtgcag
     4021 tccatcggcg tcaacggcca ggcggtcaat gacggggaga cgatcagcgg catctgggtc
     4081 tcatcggtga agtccggctc tccggctgac gcggccggcg tccagggcgg cgacatcatc
     4141 accaccatgg agggcctcgt cctctcgacc gacgggacca tggcggacta ctgcgacatc
     4201 ctgcgcagcc acgacgccga ggacaccctg gccatcgagg tcctgcgatt cgcctccgag
     4261 gaggtcctcg agggccagct caacggccgg gccctcgaga cgagcttcag cttcgcgcag
     4321 gagctcggcg acgaggtcga cgagaacggc gggggcggcg gatcgaccgg ctactccgac
     4381 ttcgtcgagg tgcaggacga cagcggcgcg atcggcatgg tggtaccggc cgagtgggcg
     4441 gaggtcgacg ggtcggcgtg gatcgccggt gagtcgaacg tcggtcccgc gatcatcgcc
     4501 acgacgagca tggacggctg gaacaacggc tgggacgtgc cgggcatctt cttcggtgcc
     4561 tcgtcgtccc tgcgctcgtc gtacgacgtc cagggcatgc tcgacgcgaa cgacttctcg
     4621 gacacctgca cgtacgagtc gcggagcgac tacgaggacc cgctgtacac cggggcctac
     4681 gacgtctacc gcgactgcgg cggaagctcg aacaccttcg tggtgctcgc ggcggagccc
     4741 gaggacgggt cgttcatcat gctcctgcag gtggtgctgg tcagcgaggc cgatgcgcag
     4801 gcgctcgacc agatcctgaa caccttccag gtgatcgggt cgctctaggc tcgaacgtcg
     4861 tcacccgaga ggcccgcccg ggatgtgccg ggcgggcctt cttcgtgggg agcgcagcgc
     4921 gcctacggcg cgtccaactg gcccggctcc ggcggcggga tgagcgggtc gctgggcggg
     4981 aggggcggtg gggcgaccga cggggtcgcc gccgagcgct gcgtgtccat gccgctggcg
     5041 ctcgcgccac cctgggccgt cgcggcggcc gcggtcaccg acgtcgcctc ggccgtccag
     5101 ggaccgtcga gcgcgaccca gtagccggcc agcggaccgt cgacgacgag tcctgcccgc
     5161 tgcccgttaa tgcgcgctga gctgctgtag cgccacgcgc tcgagctgct cggggcggtg
     5221 agcgtctccg cgatggtgcc gtcgtcgttg aagcggtagc cggtcacgtc acccgccggg
     5281 acggcgaagt tgcggcccca gtagaagtcg tagcggtcga cgaagccgcg cacgaacgcc
     5341 atgggcgtct cgcgcaccca cgtcccggcg agcggcccct cggtcaggta catgcgcacg
     5401 ccgttgcccg ggcggaccca gccgtacggc acgttgcgct gggcatacgc cacctcggtc
     5461 ggttcggtga tcaccgtgtt cgcggcggcc gactccatcc cggcgctgtc gaagcttgcg
     5521 ccgacgtgcg tgcccggcat gaggaccacc gtcccggagt ccttgaagta gggcgtgtcg
     5581 gtcacgaagg catccgccat gcgcgcgaag tcctcgtccc gcgtgtaccg gaacagctgg
     5641 atcatctggt tcgtgacgac caggtggtaa tcgctgttca cgacgttccg gtccaggcag
     5701 ctctgcgcga tgcagtagcg catcacgccg cccgggacgc gcacgatgct gagcgcgcgc
     5761 ttcgacgtcg tgaggccggc catgatcagc tgctgggcct gggagctgcc cgtcagacgc
     5821 cagtagtcgt acaggccgta gatcgcccag ttgtgcccgt tgtaggtgtg gtcgttcggc
     5881 gtccaggggt agagctcgaa ccacagcagc ccgttgtccg tcgaggtgac ccacggaccc
     5941 ccggagtcac gcccgaccag gaacgaggcg tacgtctcgt tcgcggcctg cagccaccgc
     6001 cggtcgtggg tgatctggta cagccgcacg aaaccgctga gcgcgatgcc ctgcgccatg
     6061 ccggagtacc acggcgcgcg catggtgccg cgtccgtgca ggtagaagtc gaagccgtac
     6121 gggaagaaga ccgcaccctc gtgccggacg gcgcgctcca gcagccgact tgcgttcgcg
     6181 atcgcgagat cgaggtattc ctgcttcgga tccagccggt agttgcgcag catgttgacg
     6241 acgtactgcg cctggccgac cgggtggtcg acccacgtcc cgttgtagtt ccacttgtag
     6301 acgccgttct cgagctggcc atatggcgcc acgatggtgt cgccgttgta cgccaggtac
     6361 gcatccggga cggtctcgag cgagaaggcg tcccagcgga aggcgtgggg accgatgagg
     6421 gtcagcccgc tcgccgccac ggcctggtcg atgacggcct tgctcaggac ggtcgggcca
     6481 ccgagcacga ccgtgtggtt cggacgcagg cgcgcgatct cggccgtcgt cgccgacggc
     6541 agcccggcgg tggtggacag cagcagcggg ccgcgggtgc gggcggctgc cggaacggcc
     6601 gcgacggcat cggcgaagcc caggccggtg gcgatgtggg ccgagggtgc cgacgcgaag
     6661 gccgctcggc tgatggccgc ggccgtcccg aaccgatccg cgccccacag ccgggtggcg
     6721 ccccccggcg ccatggcgtt cagctgcgcc agcaccccgt cgctcaccac ggccgcgccg
     6781 ccggcgacca cgatgcgctg cggctggagt cgtgccagct cggcgcgcac ggcgtccggg
     6841 atggaggcgg tatcgaccag gaggagtggc ccgccctggg cggcagccgc ggcgcccgcg
     6901 gcgagtgcgt ccgggaacgt ccggccggtc gccacgtacg cgaccggcac gccggccggg
     6961 aaggtccgac tgatggcgac ggccgtctcg tagcgagtgg gcccgaacag gcgcgacgtt
     7021 ggcgcgtagg tgcccagcgc ggcggccacg gcgtcggaga ccacgcccgg cccgccgagc
     7081 acgacgatgg acctgggctg caggcgggac agtgccgagc gggtggcgtc cgggagccgc
     7141 gacgcggtgg cgagcaagat cggggcgccg aggcgcgcgg ccgcgggacc cccggcgagc
     7201 gagtccggga agttctggcc ggtggcgacg aagacggccg ggaggccggg cgcgaagtcc
     7261 tcgctgacgg ccgcggccgt ggcgtaccga tcggcgccgt aggtgcgctc gatggtcgcc
     7321 tccgatgcgc gcgccggagc gaccggcacg gcggacgcga tcaggacgac gaggatgagc
     7381 agtgcgcggg cacgccggcg gcccgcctcc ccccggtgag gcatgtggtg agttcccctc
     7441 tgtgctcggt ggtgaccgcg accaccatac cgcgacgcga ggggacggcc atgggactaa
     7501 tggcacgaag tgtcgcggtg cgacctccgg cacgcccggt gcaggtgccg cgcgcatgac
     7561 ccatcgcgac gacgaccggc ctgcacccga ctcgctgcgt gggaagctcg acgacctgtt
     7621 cgacgaggac gaggacacgt cgtccgagga gacggagtcg agcctggacg acgaggcgat
     7681 cgccgacggt tcgctgccga agcgcccgac cgatccaccg ttctagcgcc ggcgcgcagc
     7741 ggtgcccggg ggtgcgtccg ctaccctgtg cccggcaggt ccgtgccgga gtggcggaat
     7801 ggcagacgca gccggcttaa acccggcggc cgcaaggcgt gggggttcga atccctcctc
     7861 cggtaccagc actcaggccg gtcgccgttc cccatcgacg atcggctcca tcgcatcctc
     7921 acccggtgga tcctcgcccg gctgcgcgac acgcggatcg gcgtagtcgg agggatcggt
     7981 ctcgctcccg agctgggctg ggtggcggtc ctcgggtgct ttgccggcgt gccgctcgta
     8041 ctcctcggtc atgcccggct cagcgcacga ggcgtgccgg agcgtcagcg acggccgaac
     8101 aggccgcgtc gctgttgccg gggctgtccc gccggcgcga cgtcgacgcc gatcacggcc
     8161 tgcgccacgc gccggagggc gagcgaggcg ggggaggccg ggcgccccat caccagcggg
     8221 acgccctcgt tgacggagcg gatcatctcc acctcggtgt aggggatctc cgccgccggc
     8281 ttggtccgga gcaggttctc gacgtcgcgg gtcttgagca gctccttcgg gaagacatgg
     8341 ttgacgacga tgtgcgtgcg cgcctgcagc ggggtggtct ccgccaggaa ggcgagcagc
     8401 aggctcatcg agcgcagggc cgcgatctcc gggaagagga cgaagacgtg cgcgtcggac
     8461 tgctccagca cccacagagc tcgcggatcg aggcggctgc ccaggtcgac cacgatctgg
     8521 tcgaaccccg gtcgcaggac ctcgacgatg cgctgaagct ggtcgagcgt cacccggaag
     8581 tcggcctcgg gacgcgcggg cgccgcgaga accgcgagcc cggactcgtg cgtggtcagg
     8641 tagctgcgcg ccagatcggg atcggccagg gcctgctcgt cgctcgccag gccggcgatg
     8701 tcgtaccgcg gcgtgacgtt gagatgcgtc gcgacctggc cgaactggag gtcgaggtcg
     8761 atcaggagga cccgggaggc ggggagcgtc ggggcacccg tggccgcggc ggcctgcggg
     8821 agcggggccc cgccggccag gaggaccgcc acgttgacgg cgagcgtcgt cgtcccgacg
     8881 ccgcccttgg ggctgaagaa cgccacgagc tccgccgtgg tccctcgcgc cgagacctcg
     8941 ggccggactc ggccggcacg gatgagcagc gccatgaccc gcgcctcgag ctcgttgcgg
     9001 ccgaacgagg cgggcaccac gtcgtcggcg ccggcctcga ggaacgcgat ccgtgcctcc
     9061 acgtcgtggg ccggtgcgac gaccaggacc ggcgtgacgc cgctgccggt cgcggttcgc
     9121 agggatccga tcgccccgga cgccgcgagc ccttccgcca cgaccaggtc cggcggcgac
     9181 ccgcgcatgg cggccaccgc gtcggccggc gtgctgaccg acgtggtgct gtagccggcg
     9241 ccgccgagcg cggtctcgag ctcggttcgc agctcggagc cgcgggcgac gatgaggatc
     9301 gggttcgagg gcatcaacgc tcgcactgtg ggtcgggtgg gccgcagtat acggtccggg
     9361 gtgatcggtg gcgccgagcg cctccgaggt ctatactcgc cccgcgcctc cggcacccgg
     9421 gggcgtggtt tctgtcagtc gaagaaccac ctttccgccg cgcgacgccc gcccggggcg
     9481 ccagcgccgc aaccccagag agaggagaca cgacgcacat gcgccgttac gagctcatgc
     9541 tcctgctccg ccccgacctg gaggacgatg ccctccagtc ggcggtcgag aaggttaccc
     9601 gcgccatcgt caacgccggc ggcagcctca ccaaggtctc gccgtggggc aagcgccgac
     9661 tcgcctacga catcaagcac ttccgtgacg ccagctactt cctgattcac ttcgacatcg
     9721 agccggcggc catccgcgag ctcgagcgcg ggatgctgat cagcgaggac atcctgcgtc
     9781 acctggtaac cgtcatcgag gaccgcgggc cggcggagga gctggcgccc gccgcggcac
     9841 ctcgcggagc caccggaggt gccggcgacg acgaggtcga ggaggccgag gaggtcgagg
     9901 acgctgacga cgagacgtcc gacgacacct cggacgatac ttccgaggac caggaggcgc
     9961 ccgcagagga cgccgccgat acggacgccg tcccgacgtc cgacgaggag cgcggcgagg
    10021 gctgaccgcc cggagccgat ggaggagccg cgatgacgct caacaagatg atgatcatcg
    10081 gcaacctggg cgccgatccc gagctgcgct acacgcccag cgggaaggcc gtcaccgacc
    10141 tccgggtcgc cgtcaacaac aaccagcgtg gcccggacgg ggagtgggtc gaggagaccc
    10201 tctggttccg ggtcagcgtc tgggagcagc aggccgagcg tctcgccgag cagctgcgca
    10261 agggcaacaa ggtgtacgcc gagggccagc tgcgcgtccg ggagttcgag ggccgcgacg
    10321 gcgagaagcg ccagtcgctg gagctcgcct tcgcccgcgt cgtcaacctc gagcgccgca
    10381 gccgcgagga cggcgagggc ggctcgtacg gcggtggtgg cggcggcggc ggatcctacg
    10441 gcgatgctcc gccgtcggtt cgcccgcgcg gtgcgcagca gcggccggga tcgtcgccgg
    10501 gcgacgagat ggacgtcgac gacatcccct tctagatcac gaggaccgat tcacatggca
    10561 agcccccgtt cccgccgcga gacggcggac tactaccgag atcgccggcg ccgcaaggtc
    10621 tgcaacttct gcgtggacaa ggcggagagc atcgactaca aggaggtcaa ccgcctccgc
    10681 cggtacctga gcgagcgcgc gaagatcgag ccgcggcgga agaccggaac ctgcgcccgc
    10741 caccagcgga tgctgaccac ggcactcaag cgagcgcgcc aggtcgcgct gctgccgtac
    10801 gcgccgcagc acctccgccc ctgaccgacc gatgacccgg gccgccgatg aggcggcccg
    10861 atcggttcct cgcccgtgct gaccgccctc ctcctcttcc tcggtggcct gggtgccggg
    10921 ctattcgggt cgctcctcgg gctggggggc gggatcctgc tcgtgccgct gctcacggtc
    10981 gggttcggat actcgatcgt gagtgccgtg ggcaccagcc tcgtgtgcgt ggtggccacg
    11041 tccgccggag cggcgtcgcg ctacgtcagg accgggcagg ccgacgttcg gctcggcatc
    11101 accctcgagg tggcgaccgc gaccggcgcc ctggccggcg gcttcatcgc cggcgtcatc
    11161 cccgagcggc tgctggccgg gttcttcgcg ctcctgctgc tgtacacggc ctacaccctg
    11221 ggccgccgag cgctcgaccc ggccccgacg ggcacggccg ccgaggcgcg ctcctgggcg
    11281 cggactggcg aagaggcgcc cgcgtaccgg acgacccggc tgccggccgc catggcgggg
    11341 tccctcggcg ccggcgtgat ctcggcgctg ctcggcatcg gcggcgggat cgtcaaagtg
    11401 cccatcgtcc acctggtgat gggggcgccg atgcacgtct cggtcgcgac gagcaacttc
    11461 atcatcggcg tcaccgccgc gaccggcgcg tacgtttacc tcttccgcgg cgagatcagc
    11521 cccgccgtgg caggaccggt cgtgctcggt gtggcgctgg gcgccgccgc cggcgcccgc
    11581 atctcgggtc gcgtcagcgg acgctggctg tcgatcatct tcctggcggt cctgctgtac
    11641 accgcgttcc agatggcgca gcgcgcgctg ggtgcggggt gagcgcgccc gccgcgccac
    11701 ccgacatgac gagggccgga cccctgcgcc ggtgggtcgc gaccgtcctg accatcggcg
    11761 tgtggagctc ggtcgcgctc gtcgcggtcg gtctgggctg ggccgtcgtc tcgggccggc
    11821 cgctcgaggt cgcccgcggc tcgctgctcg aggagctgac ggccggtgag ccgggcagcg
    11881 tcgtcctgct ggggatcctg ctcctcgtgc tgacgccgat cgcgcagctg gtggccgcgg
    11941 ccgccgcctt cgcccgcaac cgtgagcgcg ggtacgtgat cgtgacgctg gttgtgctcg
    12001 gcgtgctcgt cttcagcctg gtgctggccg ccctcacggg cgggggcgcc gagtcggcgc
    12061 gcgccgccgt cgtttgacgc gggcgcccgg ggtcggcgac actccaccgg cccgaggggg
    12121 agtacccctg acaggtgtca tcgtcaggac ggcgggacgg atcccgtccg gtggcaccgg
    12181 ccgatcgcag tggtcgggag agaccttcgg cgatcggtag gaggctgcac tggtgcccga
    12241 actcgtctcg tggtcgatct tcgcgatcgc catcttcggc ctgctcgccc tcgacctgtt
    12301 cgtcttccac cgcgaggcga agccggtcag catgcgcgag gcatcggtct gggtcaccgt
    12361 gtgggtgtcc ctgggccttg ccttcggcgc cttcatcttc ctgacgcgcg gcgccaccag
    12421 cggcggcgag tacctggcgg ggtacctcat cgagtactcg ctctcgatgg acaacgtctt
    12481 cgtcttcgcc gtcctcttcg cctacttcgc cgtgccggcg cagtaccagc atcggctgct
    12541 cttctggggc gtgctgggcg cgatcgtgtt ccgtgccgtg tttatcctgg ccggtaccgc
    12601 gctgctggag gcgttccacg tcgtggtcta cgtgttcggc gcgctgctgc tgttcaccgc
    12661 gtggcgcatg gtcacggcgg ggaccgagaa cgtggacccc aacgcgaacc cgttcctgcg
    12721 gctcatgcgc cgcgtcatcc cggtgacgga gggttaccag ggccagcgct tcttcgtgcg
    12781 ccgtgacggc cgtctgtggg cgacgccgct gttcgcggcg ctggtggtca tcgagtcgag
    12841 cgacatcatg ttcgccatcg acagcgtgcc cgccatcctc tccatcacga ccgatacgtt
    12901 catcgtcttc acctcgaacg ccttcgccat catgggcctg cggtcgctct acttcctgct
    12961 cgccgggctc atcgaccggt tcgaatacct caagtacggc ctggccgcat tgctggcctt
    13021 cgccggcgtg aagatgctgc tgagcgacgt gatccacctc gatgtctggg tctcgctcgg
    13081 catcatcgtc gcgatcctgg gcagctccat cgtgatctcg ctgatggcga cgcgatccgt
    13141 ggggagcggc gccccgcgtg gccaggtccg cgtcggttcc tccctcaccg tggcgctggt
    13201 ggtcggcgcg ctcatcgccg cgagcgcgtt gctctggctg gtgtgacggg gagcgatgga
    13261 cgtcgtcctg ctcgtcgtcg cgctgggcgt gatcctcggc tcggccgagc tgttcaccaa
    13321 cggaatcgag tggttcgggc ataagctcga cctggccgag ggtgccgtgg gcagcgtcct
    13381 ggcggcggtc gccacggcaa tgccggagac gcttatcccg accatcgcca tcgtcggtcc
    13441 ggccctgagc ggccgcgatc cccaggcgtc gcacgagatc ggcgtcggcg cgatcctggg
    13501 cgccccgttc atgctgtcga ccgtcgcgat gttcgtcacg ggggccgctg tcctggcgtt
    13561 cgcgcgccgt ggccgccgag ccgaggcgat ggacgtctcc acgccggtgc tggcgcgcga
    13621 cgtcggcttc ttcgtggtgg gctacacggt cgccatcggc acggcgttcc tgccggccgt
    13681 cgcgtggatc cggtggtcgg ccgccgccct gctgctcggc atgtacctgt actacgtccg
    13741 gctgcacttc gccgacgatc gggaggaggg ggacgcgtcg gatctcaacc ggctccacgc
    13801 cacccggctg ccgggcatcc gccgtcggtc ggcgctcgag gtccagttcc tgccggacgt
    13861 ggcggaggac gagggcgaca cgaccccacg gctcggtcac gagccacgca tgcggatcgt
    13921 ggtcggacag gtgctcgcgg ccatg
//
LOCUS       DASTYP010000191        16014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_16871, whole genome shotgun sequence.
ACCESSION   DASTYP010000191 DASTYP010000000
VERSION     DASTYP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16014
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16871"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="VFV59_08565"
     CDS             complement(<1..1748)
                     /locus_tag="VFV59_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU5325917.1"
                     /translation="MHPALQRRRRHLMMTRRTTRRGQRRRGLATFLLVTLGVFGLTFV
                     GSIMGTAGGMFAAYSYFASDLPDPNVLDGIEPAQSTYVYDRTGQTLLARFECQNREAV
                     KFAELPDIVWQATISSEDRTFWENNGVDFQGTVRAALANLEAGEIVQGASTITQQVID
                     YARVLQEEGQSTQVDEGATPAPSAIEVDPDAPAVENPEEAPQEQTDVCQPPEPKNSTD
                     FQDKIRENILAMQVTEAYPGRAGKERILETYLNLIYYGNGSYGIKAAAANYFGITDLS
                     QMSVAQAAFLAALPQAPSFLDPYQNPNGEPGSEAAAADALRERDLVIGAMQQEGYITT
                     AQAQEARRTSWLDMEPRRLTSLLLEPQFSFRVQKEAERILASLGVEDPAAAVRTGGYR
                     ITTTLDYPLQQEAKRLVAAHVHALNTSTAEEPGCLTPQGSRCQDFNVNNGALVSINSM
                     TGEIVAYVGSVDYYNREDPRVQGQFDVAGLGKRQPGSAFKPITYSSAFKSRDATASTM
                     FVDAMTEFGLGDTSYRPTNADIKEHGPVLALDALHFSLNIPSVMMQYLVGSDTTARFA
                     ESLGIASADYIMGQDPG"
     gene            complement(1797..2435)
                     /locus_tag="VFV59_08570"
     CDS             complement(1797..2435)
                     /locus_tag="VFV59_08570"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEU5325918.1"
                     /translation="MTPISIAVGLAFAVIGAASERLASVWPPDEAQRRPTGWRTVLLA
                     AASALAAVLVVLRSELPAWATGGYLVLLALLVVLTATDLEQRRLPHLVLDPLIVLAAA
                     FVPFNPAVEPAMAIIGAVAAVAFLGALALVVRGGLALGDLYLVAPIGLMLGWPVIFTA
                     LFAAAFLAAGSSLALLATRRVGMRSYIPFGPFLVGGAVLALLLDDRVLTVVR"
     gene            2637..2834
                     /locus_tag="VFV59_08575"
     CDS             2637..2834
                     /locus_tag="VFV59_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU5325919.1"
                     /translation="MDSDRIEGPLKEAGGKVKEEWGDLTDNPQTEAEGQADQAEGKLQ
                     NTWGEAKDTVRDAVDGDDDNV"
     gene            complement(2908..4383)
                     /locus_tag="VFV59_08580"
     CDS             complement(2908..4383)
                     /locus_tag="VFV59_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5325920.1"
                     /translation="MRLRATADLPATVVADVLAVPIYREDAELTGDLAELDAASGGAI
                     RAAIEWGEFNALEHPSALVDAGDLAVDRLLLLNAGARGRGPFRARRLAAVATRRLNGR
                     GARTLALWLRDGEDDDAWAAAAAGAVAGTYRSTAVYGRVRDTEAMKRSVDEVLFVGGA
                     SQDALDRGAHIGEGIAFGRDLANRSANDLSPERTAEVARELEADGCTVEILEPDDMER
                     LGMGLLLGVGQGAAHPPRLIAIKLPGWESGGDRRLAIVGKGVCFDSGGISIKPAEHMG
                     DMKHDKSGAAAVIAAARTVARLAPDTPLMAVAPMVENMPGGNAQRPGDVVKAMNGMTV
                     EVTNTDAEGRLILADAMTWAETQGATHVVDVATLTGAASVAFGDTVSAYFARPREWGE
                     RVGGAAERAGEWFWEMPLVTEYRAQYDSPHADIVNSGTRDGSLIKSAIFLSEFVTVPW
                     VHVDIAGTAYHSSDKPYGPKGATGASVATLVELALDFARKP"
     gene            complement(4434..5783)
                     /locus_tag="VFV59_08585"
     CDS             complement(4434..5783)
                     /locus_tag="VFV59_08585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5325921.1"
                     /translation="MTGPAGDPLIGRRLVDRYRVDALIARGGMARVYRARDERLDRDV
                     AVKVLSAPYADDPAFVDHFLEEARAAASLSHPSLVHVYDSGSDGGSHFFVMELLDRHR
                     TLRTAIDEQGPLPPSEVVRIGRELLAGLRVVHGRALVHCDVKTSNVMLGPGPAKLIDF
                     GIARPPDDGNDDEISLGSLHAMSPEQLRGEALTPASDLFSLGVVLYEALTGRRPFDGR
                     TPDEIGRQQDAGSVRPPSTLVAGVPGRLDAAILQALRRDPAARFHSAEAMDRALEAAS
                     RDRAVAPDDETRVVPAQRGYVPPSVPARAPAATPSPARPAPAPTPRRPSPRRRTGAFR
                     WLGTAAVLGAAALVVLLVVVPLLELGRGGGSGSPSPTPVATAPPSVEVGVVPDTVGLS
                     TPDAIELARAAGLKWTVRCNEDQTKPAGIIDQEPPAGTPASPGSAFTMYSARISDCR"
     gene            complement(5780..6628)
                     /locus_tag="VFV59_08590"
     CDS             complement(5780..6628)
                     /locus_tag="VFV59_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325922.1"
                     /translation="MRPAVAIAATVAACCVGLSADVPEAARSMDAVRLMHDRAVVVVR
                     LLDTVEARLVEATDAARAGAARTVTGEEPPGPSLEDAARLTEDAEADAAALRAAIDRL
                     RGAIQLADAGALVEQPIDRRELASVASQLRATAAAADAFATMRQRTERIPQTLDDALG
                     ALSRGDLDEAERLADAARTDHDAVSAWEVDLVTLPIWIDTTDAMIADVETIVRATRDG
                     DRAAAEAAAQAFAGRAGEATTADRALRIAIGEGGSAVTGTPLRRLADLLTRLAAARDV
                     VQGLVP"
     gene            6644..7684
                     /gene="add"
                     /locus_tag="VFV59_08595"
     CDS             6644..7684
                     /gene="add"
                     /locus_tag="VFV59_08595"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU5325923.1"
                     /translation="MSRTDALRALPKAELHQHLDGSVRPETAVELAAAVGMELTVDEA
                     RRRMAGPERCRDQAELLSYFDLPIALLQTADALERVTRELIEDLAADGIRYAEIRWAP
                     RLHLERGLSVPAVLEAVAQGVAEAALVHGPAGPRVGLIVTAIRSHPPAANVALAEAAA
                     DFGLPIVGFDLAGPESAWPAPPHAAAFRAARDGGLALTCHAGEIAGAERVREVLDFGV
                     RRIAHGVTAIADPTLVDLLRERDVTLDLCPTSNVQAGIVAELAEHPIAALHRAGVSVT
                     INTDDRTVTGTTLTDEMTRTAAAVGLSDGELASIAVNAFRRAFAPRDVVAPVLDEAVR
                     AWEAWRGGGAIS"
     gene            complement(7677..8543)
                     /locus_tag="VFV59_08600"
     CDS             complement(7677..8543)
                     /locus_tag="VFV59_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEU5325924.1"
                     /translation="MSPLPERVRLVVFDLDGVIYRGTEPIAGAPDLVGWLHERGVAVR
                     FATNNSMVERRGYVTRLAAMGIPTVEDEIVTSTSASIEHLRRHVPDVRRILAIGAAGM
                     RTELLEAGYDAVMADEVARDAAKEGIPDGGPLTDRYDAVLVGLDPQVDYARLAVAMSA
                     VEAGARLIATNADTRYPTPAGFLPGAGSIVAALATATGVTPEVIGKPAPAMFTAIFEA
                     SGVDAAHAVVVGDNPDADVAGAHRAGCAAILVLTGVASAEAADALEGERRPDAVAAGP
                     AEVRRLLEDRLS"
     gene            8735..9883
                     /locus_tag="VFV59_08605"
     CDS             8735..9883
                     /locus_tag="VFV59_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5325925.1"
                     /translation="MAKHTRAVMAFLAVLSLILAACSGGGSSPSASASAQESNPASES
                     AAPSESAAPSESAAAIDYKVCMVSDTGGFDDNSFNENGLAGLERARDELGVEIATLQS
                     NSAEDYERNINQFISDGCNMIVTVGFLLGDATKAAAEANPDVRFAIVDFAYDPPLDNV
                     EGLVYATAEAAMLAGYASASWTKTGVMGTFGGINIGPGVTDFMDGFIAGMNYWNEQKG
                     DDVRMLGGRDPNDAASGTFTGNFDSTDDAKNVTLGFLQEGADVILPVGGPIGQGTFAA
                     IREQGAEAVGLGVDVDWTQTVPDYADLMLVSIIKRIDNSVFQAIERAVNDEAPEATFL
                     SNLENEGVGISDFHDYDDQISQETKDEIQTLADAIIAGDISPSDYYGE"
     gene            9970..11550
                     /locus_tag="VFV59_08610"
     CDS             9970..11550
                     /locus_tag="VFV59_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325926.1"
                     /translation="MKLELRGITKRFPGVVANEGVDLVVNPGEIVGLLGENGAGKTTL
                     MNVLYGLYQPDEGEILIDDKPVRFAGPSDAIAAGIGMVHQHFMLVPVFTVTENVMLGV
                     ESTRPPFGRLDEPAARRRIMDISERHGLKVDPDAVVEDLPVGVRQRVEIIKTLYRFSD
                     VLILDEPSAVLTPQETEDLFGVMRSLIEHGTGIIFITHKLDEVLTVADRISVLRRGKV
                     VGETTPAETSEEELAAMMVGRSVELVVHKEPAQPKAPVLEINDLVVRDERGHAAVNEL
                     DLTVRAGEILAVAGVQGNGQTELVQAITGLRPRDSGSIRIDGQEMDGTPREIYKAGVA
                     HVPEDRLEDGLVVDFSVAENLVLNNYWTDTFANGLNLDRDAIASAADERVRDFDIRTP
                     SIFNPVGNLSGGNQQKVIAAREFSRPGVKLVVASQPTRGLDVGSIEYIHKRIVERRDA
                     GAAVLIVSSELDEVTALGDRIAVMYQGKIVATLSPEEATFERLGLLMGGAHPEDQVAS
                     PELAAEVLAAPAGDARHD"
     gene            11543..12853
                     /locus_tag="VFV59_08615"
     CDS             11543..12853
                     /locus_tag="VFV59_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325927.1"
                     /translation="MTDDTFRRQPDQEEPAPEQPMLASEPDQAVAPEPERGGRGGMRV
                     LRALLLPVLAVFSAFVVGAIIIIFSDAQTLSLLGTDLGAAVGRAWSIVMQSYGALLSG
                     SIGDPVRLLSALFAGDWVEVKRAFGPLSETIVSATPLIFTGLSVALAFRVGLFNIGAE
                     GQMYIGALIAIIAGFSFVGLPWFIHAPLAVLAGFIGGALWGFIPGLLKARTGAHEVIV
                     TIMLNFVAYRTVDYALKTAFVQRPGRSDPISKVAEPGSWLMPIVEGLRAHWGIAIAVL
                     AAVAVWWLLFRSRWGFEFRAVGLNPRAARYAGMSPGWSTVMSMVIAGGLAGLAGASLM
                     LGGSSKSLSPGFSPGYGFDGITVALVGATRPAGVVAAAFLFGALRAGATPMEAATGTP
                     RDLVVVIQALVILFIAAPALVRAIYRIRTQRTLGTEVFAKGWGS"
     gene            12850..14130
                     /locus_tag="VFV59_08620"
     CDS             12850..14130
                     /locus_tag="VFV59_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325928.1"
                     /translation="MTAAVATPRTLTLAERQARTRQRVLGVIYLIAAVVMTIGFALPV
                     AGDAVSSFNLNLGGSPIQLPPLDLPAKGTGFLLAGAVAFAGAIQLTRGFGARWALVLG
                     LVATAFVFGFLVWVAAGQSMNLTGVLQSTLQRAVPIVFGALAGVLCERSGVVNIAIEG
                     MLLTGAFVAAVIGSVTGNSWIGMLAAVLAGGLMAGILAVLAIRYRVDQIIAGFFINIF
                     ATGLTSFLAVRWLSPYQDALNRPEVLGAIPIPFLADIPILGPIFFRHNIFVYMLFGLI
                     IAIHLGLFYTRWGLRVRAVGEHPKAADTVGINVYWVRYSNVILGGMVAGLGGAFFTIG
                     STGGFEREMSAGRGYIGLAAMIFGGWNPIGAFLAGLVFGFADAVQSRLSILNIGIPSE
                     FLLMVPYVVTIIVVAGLVGRTRAPAADGKPYIKE"
     gene            14130..15077
                     /locus_tag="VFV59_08625"
     CDS             14130..15077
                     /locus_tag="VFV59_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU5325929.1"
                     /translation="MPAARRRVVLDCDPGHDDAIAILLAAASPSVDLEAIITVAGNQT
                     VDKTTLNARRVCSVAGLTDVPIAAGCDRPLVRERIVAGEIHGASGLDGVDWDEPTVGT
                     DPRDGVDLLIELARRQDPRPLTVVAVGPLTNVATALQRAPDIAGRIDRISIMGGAIGL
                     GNWTPSAEFNIYADPEAADVVVNAGVPVTLVPLEVTHLALATEPVLDRIAALDTPVAR
                     MSDALLRFFTDTYREVFGFPAPAVHDPCAVAAVIDPSIVPTRHLNVAIDTASALSAGR
                     TVVDVYGTTGREPNADVAVGLEVERFWDLVLEALATYAG"
     gene            complement(15065..15841)
                     /locus_tag="VFV59_08630"
     CDS             complement(15065..15841)
                     /locus_tag="VFV59_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5325930.1"
                     /translation="MRIAVMGDVHANLPALRAVIDDVVRIGADALYCVGDVVGRGPHP
                     NEVVDELRRLEIPTIQGNWDEAVGMDRDQNGAAWTTIEAEADGNRSMRWTAETLTDEN
                     RAWLRQLPATLRLTLAGRSLYFFHGSPLRQGEYLWNDRPSRVFARIASDEADDVFCFG
                     HTHEAWHRVVGQAHFVACGSVGCGTEGDARARYAVVYLGEPDIAVGFRSVDYDHVAVV
                     RDLAATGCSVDLLRQPPSRHPHVPSEAAPAWRERPGAQPA"
BASE COUNT         2240 a         5766 c         5710 g         2298 t
ORIGIN      
        1 cccgggtcct ggcccatgat gtagtcggcg gacgcgatac cgagcgactc ggcgaagcgc
       61 gccgtcgtat cggatccgac caggtactgc atcatcaccg aggggatgtt cagcgagaag
      121 tgcagcgcgt cgagcgccag caccggcccg tgctccttga tgtcggcgtt cgtgggccgg
      181 tagctcgtgt cgccgaggcc gaactcggtc atggcgtcga cgaacatcgt cgaggccgtc
      241 gcgtcacggg acttgaaggc cgatgagtac gtgatcggct tgaaggcgga tcccggctga
      301 cgcttgccca ggccggcaac gtcgaactgg ccctgcaccc gcgggtcctc gcggttgtag
      361 tagtcgacgg agccgacgta cgcgacgatc tcgccggtca tcgagttgat cgaaacgagc
      421 gccccattgt tgacgttgaa gtcctggcag cggcttccct ggggcgtgag gcagccgggc
      481 tcctcggcgg tgctcgtgtt cagcgcatgg acgtgcgcgg ccaccagccg cttggcctcc
      541 tgctgcagcg ggtagtccag cgtggtcgtg atccgatacc cgccggtgcg caccgccgcc
      601 gcgggatcct cgacgcccag gctggccagg atgcgctccg cctccttctg aacccggaag
      661 ctgaactgcg gctcgagcag gaggctcgtc aggcggcggg gctccatgtc gagccagctc
      721 gtccgacgtg cctcctgtgc ctgggccgtc gtgatgtagc cctcctgctg catggcgccg
      781 atgaccaggt cgcgctcgcg cagcgcatcg gccgcggccg cctcgctgcc gggctcgccg
      841 ttcgggttct ggtacggatc caggaaggac ggcgcctgcg gcagggcggc caggaaggcg
      901 gcctgcgcca ccgacatctg gctcaggtcg gtgatgccga agtagttggc cgccgccgcc
      961 ttgatgccgt acgagccgtt gccgtagtag atgaggttca ggtaggtctc gaggatccgc
     1021 tccttgccgg cgcggcccgg gtaggcctcg gtcacctgca tggccaggat gttctcgcgg
     1081 atcttgtcct ggaagtcggt cgagttcttg ggctccggcg gctggcagac atcggtctgc
     1141 tcctgggggg cctcctccgg gttctcgacc gccggcgcgt ccgggtcgac ctcgatggcg
     1201 ctgggtgcgg gtgtcgcgcc ctcgtccacc tgggtcgact gcccctcctc ctggaggacg
     1261 cgggcgtagt cgatgacctg ctgcgtgatg gtcgaggcgc cctgcacgat ctcccccgcc
     1321 tccaggttcg ccagcgccgc gcgcaccgtg ccctggaagt cgacgccgtt gttctcccag
     1381 aaggtgcggt cctcgctgga gatcgtcgcc tgccacacga tgtcgggcag ctcggcgaac
     1441 ttcacggcct cgcggttctg gcactcgaag cgggcgagca gggtctggcc ggtgcggtcg
     1501 tagacgtagg tcgactgcgc cggctcgatg ccgtcgagga cgttgggatc aggcaggtcc
     1561 gaggcgaagt agctgtaggc ggcgaacatc ccgcccgccg ttcccatgat cgagccgacg
     1621 aaggtcaggc cgaagacgcc cagggtgacg agcaggaagg tcgcgaggcc ccgccgacgc
     1681 tgcccgcgac gcgtggtgcg ccgggtcatc atgaggtggc gacgccgacg ctggagagcc
     1741 ggatgcatcg ggcgcagggt accagcgaac gggtgttcgg tcgaatgcgg ttccgatcag
     1801 cgaaccacgg tcagcacgcg gtcgtcgagc agcagcgcca ggacggcgcc ccccacgagg
     1861 aacgggccga atgggatgta gctgcgcatc ccgacccgac gcgtggcgag cagggccagg
     1921 ctgctgccag ccgccaggaa cgcggcggcg aagagggccg tgaagatcac cggccagccg
     1981 agcatgaggc cgatgggcgc caccaggtac aggtccccca gcgccagccc accgcgcacg
     2041 acgagcgcca gcgcacccag gaatgccacg gccgccacgg cgccgatgat ggccatggcc
     2101 ggctcgaccg ccggattgaa cggcacgaac gccgcggcaa gcacgatgag cgggtccagg
     2161 acgaggtgcg gaagtcgacg ttgctcgagg tccgtggccg tcagcacgac caacagggcg
     2221 agcagcacga ggtagccgcc ggtcgcccac gcgggcagct ccgaccggag cacgacgagc
     2281 acggcggcga gggcgctggc agcggcgagc aggacggtgc gccagccggt cggacggcgc
     2341 tgtgcctcgt cgggcggcca gacactcgcc aggcgctcgg acgctgcgcc gatgacggcg
     2401 aaggccaggc ccacggcgat gctgatcggc gtcacgctgt cctcccggga cctcctcgct
     2461 cgggccgggt cgtcgcagcc gtggtggtgc gtgccggcac cgggatgcta cttcggacgc
     2521 ccggctcgac cataggccga tcgtcccgcg gcttcggcgg cgtgctggca tgcgacgtgc
     2581 gttttcggag cagcactgcc ggcaccggcc ggcgtgatac cgcggaggtt gaatccatgg
     2641 acagtgatcg catcgagggc ccgctcaagg aagcgggtgg caaggtcaag gaagagtggg
     2701 gcgacctgac ggacaacccc cagaccgagg ccgagggcca ggcggaccag gccgagggca
     2761 agctccagaa cacgtggggc gaggccaagg acaccgtccg cgacgccgtc gacggcgacg
     2821 acgacaacgt ctgacctctc gtcaccagca ggccccggcc gtcacggtcg gggccttttc
     2881 tgttgcgtgc gtcggcgcag gcggccctca gggcttacgc gcgaagtcca gggccagctc
     2941 caccagcgtc gcgaccgatg cgccggtggc gcccttcggc ccgtacggct tgtcgctgga
     3001 gtggtacgcc gtgccggcga tgtcgacgtg cacccagggc accgtgacga actcgctcag
     3061 gaagatcgcc gacttgatca gcgacccgtc ccgggtcccg gagttcacga tgtcggcgtg
     3121 gggtgagtcg tactgggcgc ggtactcggt gaccagcggc atctcccaga accactcccc
     3181 cgctcgctcg gccgcaccgc ccacccgctc gccccactca cgcgggcgcg cgaagtaggc
     3241 gctgaccgtg tcgccgaagg cgaccgaggc cgcgccggtg agcgtcgcga cgtctacgac
     3301 gtgagtggcg ccctgcgtct cggcccaggt catggcatcg gccaggatga ggcggccctc
     3361 ggcgtcggtg ttcgtcacct cgacggtcat gccgttcatg gccttcacca cgtcacccgg
     3421 ccgctgcgcg ttgccgccgg gcatgttctc gaccatgggc gcgacggcca tgagcggggt
     3481 gtcgggcgcc aggcgcgcca cggtgcgcgc cgcggcgatg accgcggcag cgcccgactt
     3541 gtcgtgcttc atgtcgccca tgtgctcggc cggcttgatg ctgatcccgc cggagtcgaa
     3601 gcacacgccc ttgccgacaa tcgccagccg tcgatcgccg cccgactccc agcccggcag
     3661 cttgatggcg atgagacgcg gcgggtgtgc cgcgccctgg ccgacaccca ggagcaggcc
     3721 catcccgagc cgctccatgt cgtcgggttc gaggatctcg accgtgcagc cgtcggcctc
     3781 cagctccctg gcgacctcgg cggtccgctc cggcgagagg tcgttcgccg accgattggc
     3841 caggtcccgc ccgaaggcga tcccctcgcc gatgtgcgcg ccgcggtcga gcgcgtcctg
     3901 ggaggcgccg cccacgaaaa ggacctcgtc gaccgatcgc ttcatggcct cggtgtcgcg
     3961 gacacgcccg tagacggcgg tcgaccgata cgtcccggcg acggcaccgg cggcggcagc
     4021 cgcccaggcg tcgtcgtcct cgccgtcgcg cagccacagc gccagggtgc gggcaccacg
     4081 accgttcaga cgccgcgtgg cgacagccgc gagccggcgg gcgcggaacg gtccgcgacc
     4141 gcgggcaccg gcgttgagga gcagcagccg gtcgaccgcg agatcgccgg cgtccaccag
     4201 cgcggagggg tgctccagcg cgttgaactc tccccactcg atggccgcgc ggattgcgcc
     4261 gccggacgcg gcgtccagct cggcgaggtc accggtcagc tcggcgtcct cgcggtagat
     4321 cggtaccgcg agcacgtcgg cgacgacggt ggcgggaagg tcggcggtcg cgcgcaggcg
     4381 catgcttcga tgagctcctt cgtcacgggt gggacgcgcc gagtatagga gcgtcagcgg
     4441 cagtcggaga tgcgcgccga gtacatcgtg aacgcgctgc ccggcgacgc aggcgtgccg
     4501 gccggtggct cctggtcgat gatgccggcg ggcttcgtct gatcctcgtt gcagcgcacc
     4561 gtccacttca gccctgccgc gcgcgccagc tcgatggcgt cgggcgtcga gagccccacc
     4621 gtgtcgggga cgacgcccac ctccacggac ggaggagcgg tggccacggg cgtcggcgac
     4681 ggcgagccgc tcccaccgcc gcgacccagc tcgagcagcg gcacgaccac cagcagcacg
     4741 acgagcgcgg cggcaccgag gaccgccgcc gtcccgagcc agcggaaggc gccggtccgt
     4801 cggcgcggcg agggtcgtct cggggtcggc gccggagcgg ggcgggcagg tgacggcgtg
     4861 gcggccggcg ccctcgcggg cacggacggc ggcacgtacc cgcgctgggc gggcaccacc
     4921 cgggtctcgt cgtccggcgc cacggcccga tcgcgcgacg ccgcctcgag ggcgcggtcc
     4981 atggcctccg cgctgtggaa ccgggcggcc ggatcacgac gcagcgcctg gaggatcgcg
     5041 gcgtccagcc gtccggggac tcccgccacc agcgtggatg gcggtcgaac cgagcccgcg
     5101 tcctgctggc ggccgatctc gtcaggcgtg cggccgtcga acggccgccg tcccgtgagc
     5161 gcctcgtaca gcaccacgcc caggctgaag aggtcgctgg ccggcgtcag cgcctcgccg
     5221 cgcagctgtt cgggcgacat cgcgtgcagc gagcccaggc tgatctcgtc gtcgttcccg
     5281 tcgtcgggcg gccgggcgat gccgaagtcg atgagcttcg ccgggcccgg acccagcatc
     5341 acgttcgacg tcttcacgtc gcagtgcacc agcgcgcgac cgtgcaccac gcgcaggccc
     5401 gcgagcagct cacgaccgat gcgaaccacc tccgacggcg gcagcggccc ctgctcgtcg
     5461 atcgccgtcc gcagggtgcg gtgacgatcg agcagctcca tgacaaagaa gtgggagccg
     5521 ccgtcgctgc ccgagtcgta gacgtggacc agggacgggt gggagaggga tgctgcggcg
     5581 cgcgcctcct ccaggaagtg atccacgaag gccggatcgt cggcgtacgg tgccgagagg
     5641 accttcacgg ccacgtcccg atccagtcgc tcgtcgcgcg cccggtacac gcgggccatg
     5701 ccgccgcggg cgatcagggc gtcgacgcgg tagcggtcga ccaggcgccg cccgatcagc
     5761 ggatcccccg ccgggccggt cacggcacca gcccctgcac gacgtcccgg gcggcggcaa
     5821 ggcgcgtgag caggtcggcg agccgccgca gcggcgtccc ggtgacggca ctgccaccct
     5881 cgccaatcgc gatacgcagc gcccggtcag ccgtcgtggc ctccccggcg cgcccggcga
     5941 acgcctgtgc cgcggcctcg gctgcggcgc gatcaccgtc gcgcgtggcg cgcacgatcg
     6001 tctcgacgtc ggcgatcatc gcgtccgtcg tgtcgatcca gatcggcagg gtcacgaggt
     6061 cgacctccca ggcggagacg gcatcatgat cggtccgtgc cgcatcggcc agccgctcgg
     6121 cctcgtccag gtcgccgcgg ctgagggcgc cgagcgcgtc gtcgagcgtc tgtgggatcc
     6181 gttccgtgcg ttggcgcatc gtggcgaagg cgtcagcggc ggccgcggtg gcccgcagct
     6241 gcgacgcgac ggacgcgagc tcgcggcggt cgatcggctg ctcgacgagg gcgccggcgt
     6301 cggccagttg gatcgcgcct cgcaggcggt cgatggctgc gcggagcgcg gccgcatcgg
     6361 cctcggcgtc ctcggtcagg cgcgccgcgt cctccaggct gggccccggc ggctcctcgc
     6421 cggtgaccgt gcgcgccgcc ccggcccgag cggcgtcggt cgcctccacg agacgcgcct
     6481 ccacggtgtc cagcaaacgc acgacgacga cggcgcggtc gtgcatgagc cggacggcgt
     6541 ccatggatcg ggccgcctcc gggacgtcgg cggacagccc gacgcagcag gcggcaacgg
     6601 tggccgcgat ggcgaccgcc gggcgcatcc ctatcatggg ccgatgtccc gcaccgatgc
     6661 gctgcgcgcc ctccccaagg ccgagctgca ccagcacctg gatgggtccg tgcggccgga
     6721 gacggccgtc gagctcgccg ccgccgtggg catggagctc accgtggacg aggcgcgccg
     6781 ccggatggcg gggcccgagc gctgccgcga ccaggcggag ctgctgtcgt acttcgacct
     6841 gccgatcgcc ctgctgcaga ccgccgacgc gctcgagcgc gtgacgcgcg agctgatcga
     6901 ggacctcgcc gcggacggca tccggtacgc ggagatccgg tgggcgccac gcctgcacct
     6961 cgagcgcggg ctctccgtcc cggcggtcct cgaggcggtg gcgcaggggg tggcggaggc
     7021 ggcgctggtc cacggtccgg ccgggccacg ggtcgggctg atcgtgaccg ccatccgttc
     7081 ccacccgccg gccgccaacg tggccctggc ggaggccgcg gccgacttcg gcctgccgat
     7141 cgtcggcttc gacctcgccg gcccggagtc ggcctggccg gcaccgccac acgcggccgc
     7201 gttccgcgcc gcgcgcgatg gcggcctggc gctcacctgc cacgccggtg agatcgccgg
     7261 cgccgagcgc gtgcgcgagg tcctggactt cggcgtgcgc cgcatcgcgc acggcgtgac
     7321 cgccatcgcg gaccccaccc tggtcgacct gctgcgggag cgcgacgtga cgctcgacct
     7381 gtgcccgacc tccaacgtcc aggccgggat cgtcgctgag ctggcggagc atccgatcgc
     7441 ggcgctgcac cgcgccggcg tgtcggtcac gatcaacacc gacgatcgaa ccgtcaccgg
     7501 caccaccctg accgacgaga tgacccggac ggcagcagcg gtcggcctgt cggacggcga
     7561 gctggcgtcg atcgcggtca acgcgttccg gcgcgcgttc gcgccgcgag atgtcgtggc
     7621 accggtgctg gatgaggccg tccgtgcgtg ggaggcgtgg cgcggcggcg gcgccatcag
     7681 ctgagccggt cctcgagcag ccggcgcacc tcggcggggc cggcggccac ggcatcgggg
     7741 cgtcgctcgc cctccagcgc atcggccgcc tcggcgctcg cgacgcccgt cagcaccagg
     7801 atcgcggcac agcccgcgcg gtgcgctccg gccacgtcgg catccgggtt gtcgcccacg
     7861 accaccgcgt gcgcagcgtc gacgccgctc gcctcgaaga tggccgtgaa catcgcgggc
     7921 gccggcttcc cgatcacctc gggcgtgacg ccggtcgccg tggccagcgc cgcgacgatc
     7981 gagccggcgc cgggcaggaa ccccgccggc gtcgggtagc gcgtatcggc gttcgtggcg
     8041 atcaggcggg cgcctgcctc gaccgcgctc atcgcgaccg ccagccgcgc gtagtcgacc
     8101 tgcgggtcga ggccgacgag gacggcgtcg tatcggtccg tcagcggtcc gccgtccggg
     8161 atcccctcct tggcggcatc gcgcgccacc tcgtccgcca tgacggcgtc gtaccccgcc
     8221 tccagcagct cggtccgcat ccccgcggcg ccgatggcga ggatgcggcg cacgtccggc
     8281 acgtggcgcc gcaggtgctc gatggaggcg gaggtcgagg tcacgatctc gtcctcgacg
     8341 gtcgggatgc ccatggcggc gagacgcgtg acgtagccgc gacgctcgac catcgagttg
     8401 ttggtcgcga agcggaccgc cacgccgcgc tcgtggagcc acccgaccag gtcgggggcg
     8461 cccgcgatcg gctccgtccc tcggtagatg accccgtcca ggtcgaagac gacgaggcgg
     8521 acgcgttccg gcagcggact cacgcggccg atcataggcc cgccgccggc cgagacggag
     8581 accgggggcg accggaccgt gccgaaagac ccttgaggac gggtaaagcg cttcagtaca
     8641 atgccgcgga gtctgcgcca ggtgcccact ggcccgagcg catctgtgcg ccgcgatcga
     8701 cgcggctcgc accggacaat ggtgtggagg agaaatggcg aagcacactc gggcagtgat
     8761 ggccttcctg gccgtgctgt ccctcatcct cgcggcgtgc agcggcggcg ggtcgagccc
     8821 gtcggccagc gcgagcgcgc aggagtcgaa cccggccagc gagagcgcgg caccgagcga
     8881 gagcgcggca ccgagcgaga gcgccgcggc catcgactac aaggtctgca tggtgtccga
     8941 caccggcggc ttcgacgaca acagcttcaa cgagaacggc cttgccggcc tcgagcgcgc
     9001 gcgcgatgag ctgggcgtcg agatcgcgac gctgcagtcg aacagcgccg aggactacga
     9061 gcgcaacatc aaccagttca tcagcgacgg ctgcaacatg atcgtgaccg tcggcttcct
     9121 cctcggcgac gccaccaagg cggccgcgga ggccaacccc gacgtccgct tcgcgatcgt
     9181 cgacttcgcc tacgacccgc cgctcgacaa cgtcgagggc ctcgtctacg cgacggccga
     9241 ggcggccatg ctggccggct acgcgtcggc gtcgtggacc aagaccggcg tcatgggcac
     9301 gttcggcggc atcaacatcg gccccggcgt caccgacttc atggacggct tcatcgccgg
     9361 catgaactac tggaacgagc agaagggcga cgacgttcgc atgctcggcg gccgcgaccc
     9421 gaacgatgcg gcgtccggca ccttcaccgg caacttcgac agcaccgacg acgccaagaa
     9481 cgtcaccctg ggcttcctcc aggagggcgc tgacgtcatc ctgcccgtcg gtggtccgat
     9541 cggtcagggc accttcgccg ccatccgcga gcagggtgcc gaggcggtcg gcctcggcgt
     9601 ggacgtcgac tggacccaga ccgtccccga ctacgccgac ctgatgctcg tcagcatcat
     9661 caagcgcatc gacaactcgg tcttccaggc catcgagcgc gccgtcaacg acgaggcgcc
     9721 cgaggccacc ttcctctcga acctcgagaa cgagggcgtc ggcatcagcg acttccacga
     9781 ctacgacgac cagatctcgc aggagaccaa ggacgagatc cagacgctgg ccgatgcgat
     9841 catcgccggc gacatctccc cgtccgacta ctacggcgag taacggcttc gacccgatcg
     9901 ggttgagtat gctcagcggg cggcgtcctc cgcggacgcc gcccgttcgg taccacgcgt
     9961 gaggcgtccg tgaagctcga gctgcggggg atcaccaagc ggttccctgg cgtcgtcgcc
    10021 aacgagggcg tcgacctcgt cgtcaacccc ggcgagatcg tcggcctgct cggcgagaac
    10081 ggcgccggca agacgacgct catgaacgtc ctctacggcc tctaccagcc ggacgagggc
    10141 gagatcctga tcgacgacaa gccggtgcgc ttcgccggtc cgtcggacgc gatcgcggcc
    10201 ggcatcggca tggtccatca gcacttcatg ctcgtgcccg tcttcacggt caccgagaac
    10261 gtcatgctcg gcgtcgagtc gacgcggccg cccttcgggc gcctggacga acccgccgcc
    10321 cggcgccgca tcatggacat cagcgagcgc cacggcctca aggtcgaccc ggacgcggtg
    10381 gtcgaggacc tgcccgtcgg cgtgcggcag cgcgtggaga tcatcaagac cctctaccgc
    10441 ttctcggacg tcctgatcct ggacgagcca tccgcggtgc tcacaccgca ggagaccgag
    10501 gacctgttcg gcgtcatgcg ctcgctgatc gagcacggca cgggcatcat cttcatcacc
    10561 cacaagctcg acgaggtgct caccgtcgcc gaccggatca gcgtcctgcg ccgtggcaag
    10621 gtcgtcggcg agacgacgcc ggccgagacc agcgaggagg agctggcggc gatgatggtc
    10681 ggtcgcagcg tcgagctggt ggtccacaag gagccggcgc agccgaaggc gcccgtgctc
    10741 gagatcaacg acctggtggt ccgtgacgag cgcggccacg ccgcggtcaa cgagctcgac
    10801 ctgaccgtgc gggccggcga gatcctggcc gtggccggcg tgcagggcaa cgggcagacg
    10861 gagctggtcc aggccatcac cggcctccgg ccgcgtgact ccgggtcgat ccggatcgac
    10921 gggcaggaga tggacggcac cccgcgcgag atctacaagg ccggcgtcgc acacgtcccg
    10981 gaggatcggc tcgaggacgg cctggtcgtc gacttctcgg tggcggagaa cctggtcctg
    11041 aacaactact ggaccgatac cttcgcgaac ggcctcaacc tcgaccgcga cgcgatcgcg
    11101 tcggcggccg acgagcgcgt gcgcgacttc gacatccgga cgccgtcgat cttcaatccg
    11161 gtcggcaacc tgtccggcgg caaccagcag aaggtcatcg ccgcccgcga gttctcgcga
    11221 ccgggcgtga agctggtcgt cgcctcgcag ccgacgcgcg gcctcgacgt cggctcgatc
    11281 gagtacatcc acaagcgcat cgtcgagcgg cgcgacgccg gagccgccgt cctcatcgtc
    11341 tcgagcgagc tggacgaggt cacggccctc ggcgaccgca tcgcggtcat gtaccagggc
    11401 aagatcgtcg cgacgctgtc gcccgaggag gcgaccttcg agcggctcgg cctgctgatg
    11461 ggcggcgcgc acccggagga ccaggtcgcg tcgcccgagc tcgccgccga ggtcctcgcc
    11521 gctccggccg gagacgcacg ccatgactga cgacacgttc cggcgtcagc cagaccagga
    11581 ggagccggca ccggagcagc cgatgctggc cagtgagccg gaccaggcgg tggccccgga
    11641 gccggagcga ggcggacgtg gcggcatgcg cgtcctgcgg gcgctgctcc tccccgtcct
    11701 ggccgtcttc tcggccttcg tcgtgggcgc gatcatcatc atcttcagcg acgcgcagac
    11761 gctgagcctg ctcggcaccg atctgggggc ggcggtcgga cgcgcctgga gcatcgtgat
    11821 gcagtcgtac ggagcgctgc tgagcggctc catcggcgac ccggtgcggc tgctgagcgc
    11881 gctcttcgcc ggcgactggg tcgaggtcaa gcgggccttc ggtccgctgt cggagaccat
    11941 cgtgtcggcg acgccgctga tcttcaccgg cctctcggtc gcgctggcgt tccgcgtcgg
    12001 cctcttcaac atcggcgccg aaggccagat gtacatcggc gcgctgatcg cgatcatcgc
    12061 cggcttctcg ttcgtgggcc tgccgtggtt catccacgcg ccgctcgccg tcctcgcggg
    12121 cttcatcggc ggcgccctgt gggggttcat ccccggcctg ctgaaggccc ggaccggtgc
    12181 ccacgaggtg atcgtcacga tcatgctcaa cttcgtcgcc taccggacgg tcgactacgc
    12241 gctgaagacg gccttcgtgc agcgccccgg ccgctccgac ccgatcagca aggtcgcgga
    12301 gcccgggtcg tggctgatgc cgatcgtcga agggctgcgc gcccactggg gcatcgccat
    12361 cgccgtcctc gcggccgtgg ccgtctggtg gctgctgttc cgctcgcgct ggggcttcga
    12421 gttccgggcc gtcggcctca acccccgtgc cgctcgctac gccggcatga gccccggctg
    12481 gtccacggtg atgtcgatgg tcatcgccgg cggcctggcg gggctggcgg gcgcgagcct
    12541 gatgctcggt ggctcgtcga agagcctgag ccccggcttc tcaccgggct acggcttcga
    12601 cggcatcacc gtcgccctgg tcggcgccac gcggcccgcg ggcgtcgtgg ccgcggcctt
    12661 cctcttcggg gcgctgcgcg ccggggcgac gcccatggaa gccgccaccg gcaccccgcg
    12721 tgacctggtg gtcgtgatcc aggcgctggt catcctcttc atcgcggcac cggcgctggt
    12781 gcgcgcgatc taccgcatcc gcacccagcg gacgctcggg accgaggtgt tcgccaaggg
    12841 gtggggctcg tgaccgccgc cgttgccacg ccgcgcacgc tgacgctggc cgagcgccag
    12901 gctcgcaccc ggcagcgggt gctgggggtc atctacctca tcgccgccgt cgtcatgacc
    12961 atcggcttcg cgctcccggt cgccggcgac gcggtctcct cgttcaacct caacctcggc
    13021 ggatcgccga tccaactgcc gccgctcgac ctgccggcca aggggaccgg cttcctgctc
    13081 gccggggcgg tcgccttcgc cggcgccatc cagctgaccc gcggcttcgg cgcgcgctgg
    13141 gcgctcgtcc tgggcctggt ggccacggcc ttcgtgttcg gcttcctggt gtgggtcgcc
    13201 gccgggcagt cgatgaacct gaccggggtg ctgcagagca cgctccagcg ggccgtcccc
    13261 atcgtcttcg gtgcgctcgc cggtgtgctg tgcgagcgct cgggcgtggt gaacatcgcc
    13321 atcgagggga tgctgctgac cggtgccttc gttgccgcgg tcatcggctc ggtcaccggc
    13381 aactcctgga tcggcatgct cgccgccgtc cttgccggcg gcctgatggc cggcatcctg
    13441 gccgtgctgg cgatccgcta ccgggtcgac cagatcatcg ccggcttctt catcaacatc
    13501 ttcgccaccg gcctgacctc gttcctggcc gtgcgctggc tctcgccgta ccaggacgcg
    13561 ctgaaccgtc ccgaggtcct cggcgccatc ccgatcccct tcctggcgga catcccgatc
    13621 ctcggcccga tcttcttccg gcacaacatc ttcgtgtaca tgctcttcgg gctgatcatc
    13681 gccatccacc tcgggctctt ctacacgcgc tggggcctgc gggtgcgcgc cgtgggcgag
    13741 catcccaagg cggccgatac ggtcggcatc aacgtctact gggtgcggta cagcaacgtg
    13801 atcctgggcg gcatggtcgc cggcctcggc ggcgccttct tcaccatcgg ctccaccggt
    13861 ggcttcgagc gcgagatgag cgccgggcgc ggctacatcg gcctggcggc catgatcttc
    13921 ggcggctgga atccgatcgg cgccttcctg gccggcctgg tcttcggatt cgccgatgcg
    13981 gtccagtcgc gcctctcgat cctgaacatc ggcatcccgt ccgaattcct gctgatggtg
    14041 ccgtacgtgg tgaccatcat cgtggtggcc gggctcgtcg ggcgcacccg cgcccccgcg
    14101 gccgacggca agccgtacat caaggagtga tgccagccgc gcgtcggcgg gtggtcctgg
    14161 actgcgatcc cggccacgac gacgccatcg ccatcctgct ggcggccgcc tcaccgtcgg
    14221 tcgacctgga ggcgatcatc acggtcgccg gcaaccagac cgtcgacaag acgacgctca
    14281 acgcgcggcg cgtgtgctcg gtggccggcc tcaccgacgt gcccatcgcc gccggctgcg
    14341 accggccact ggtgcgcgag cggatcgtgg cgggcgagat ccatggcgcc tcagggctgg
    14401 acggcgtcga ctgggacgag cccacggtcg ggacggaccc gcgcgacggc gtcgacctgc
    14461 tgatcgagct ggcccggcgc caggatccgc gtccgctgac cgtggtcgcc gtcgggccgc
    14521 tcacgaacgt cgccaccgca ctgcagcgag cgccggacat cgcgggccgc atcgaccgca
    14581 tctcgatcat gggcggggcg atcgggctgg ggaactggac gccgtcggcc gagttcaaca
    14641 tctacgcgga ccccgaggcg gcggacgtgg tggtcaacgc cggcgtgccg gtgaccctcg
    14701 tcccgctgga ggtgacgcac ctggccctcg ccaccgagcc cgtgctggac cgcatcgccg
    14761 cgctggacac gccggtcgcg cgcatgagcg acgcgctgct gcgcttcttc accgacacgt
    14821 accgcgaagt cttcggcttc ccggcgccgg ccgtccacga cccgtgcgcc gtggccgcgg
    14881 tcatcgatcc gtcgatcgtg ccgacccgcc acctgaacgt ggcgatcgac acggcctcgg
    14941 cgctcagcgc cgggcgcacc gtggtcgacg tgtacggcac cacgggacgg gagccgaatg
    15001 ccgacgtggc ggtcgggctt gaggtcgagc gcttctggga cctggtgctc gaggcgctgg
    15061 cgacctacgc gggctgagcc ccggggcgct cacgccaggc cggcgcggcc tcggacggga
    15121 cgtggggatg gcgcgagggc ggctgccgga ggaggtcgac ggagcagccg gtcgcggcga
    15181 ggtcgcgcac gaccgccacg tggtcgtagt cgaccgaccg aaagccgacg gcgatgtcgg
    15241 gctcacccag gtagacgacc gcgtaccgtg cgcgggcgtc accctccgtg ccgcagccga
    15301 ccgagccgca cgccacgaag tgcgcctgcc cgacgacgcg gtgccaggcc tcgtgcgtgt
    15361 ggccgaagca gaagacgtca tccgcctcgt ccgacgcgat gcgggcgaag acgcgcgacg
    15421 gtcggtcgtt ccagaggtac tcgccctgcc gcagcggtga gccgtggaag aagtacagcg
    15481 atcgcccggc cagggtgagc cgaagggtcg ccggcagctg gcgcagccag gcgcggttct
    15541 cgtcggtcag cgtctccgcg gtccagcgca tcgagcggtt gccgtccgcc tccgcctcga
    15601 tcgtcgtcca cgccgcgccg ttctggtcac ggtccatgcc gaccgcctcg tcccagttgc
    15661 cctggatcgt ggggatctcc agccgacgga gctcgtccac cacctcgttg gggtgtggcc
    15721 cgcgccccac cacgtcgccg acgcagtaga gcgcatcggc gccgatccgc acgacgtcgt
    15781 cgatcaccgc ccgcagcgcc ggcaggttcg cgtggacgtc ccccatcacc gcgatccgca
    15841 tgccgggatt ctacggtccc gtccaggtcc gacaatgggc cttccgggtg accatcgtct
    15901 catccggcac ggacctcgca ggcgtggagc gacgtggact ggagctggtt ctgggcgacc
    15961 gatgcgagcg tggcgcgcct cgtcatcgag cgcggcatcg ccctggtcta cctg
//
LOCUS       DASTYP010000192         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1802276, whole genome shotgun sequence.
ACCESSION   DASTYP010000192 DASTYP010000000
VERSION     DASTYP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802276"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1378
                     /gene="fabF"
                     /locus_tag="VFV59_08635"
     CDS             110..1378
                     /gene="fabF"
                     /locus_tag="VFV59_08635"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5325931.1"
                     /translation="MTTTRASRDPGRRAVVTGLGAITPIGNDVATFWQNLTDGVSGVA
                     EIASFDASALEVRIAAEVKGFEPKDWMDFKQARRMSRFAQLAVAAARQAIDDSGLQIG
                     DHNRDDVAVVINTGGGGLQEVAVGETTYLERGPDRVSPFMVPMMSPSMAACQVSIQNG
                     IRGPVITSVAACASGVQAFVEAQRLIERGEVDVVIAGGTESAIMPVSFAALANMGALS
                     KRNDDPTRASRPFDANRDGFVFGEAAGVVVVESAAHAAARGANVIAEVAGGALTGDAF
                     HISAPDPAGFGATLAMRRALRDSGLEPSEVDHVVAHGTSTPLNDATETKAIHAAFGDD
                     AARLAISSNKSMLGHTLGAAGAVSGLQAILAIRDGVVPPTINLETPDPACDLDYIPNV
                     ARRMPVNVAMINGFGFGGQNAVAVFRRFQA"
     gene            1456..1935
                     /locus_tag="VFV59_08640"
     CDS             1456..1935
                     /locus_tag="VFV59_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325932.1"
                     /translation="MTEVNGLRLAYRGFVAGLAGAYVWIAVAMLLSLPTGGPMAPVQA
                     LGSIGPEGARTSTSEALVLGLGLVQVAGAGIGMAFAYFFGRFFTVRATLGTAAVCVAI
                     LAWSLLGSRIGIAIGVNEVDLVTSLALLAATIGYGWVVGAWVPVRGEVLRYDSSPVT"
     gene            complement(1911..2501)
                     /locus_tag="VFV59_08645"
     CDS             complement(1911..2501)
                     /locus_tag="VFV59_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5325933.1"
                     /translation="MPERDASNTYPVTLGEEPEFCPACGERVEPRVLEAHHRPRLVCP
                     NGHVTWRNPRIVVGTLPVDGGRVFLARRGIEPALGRWSYPGGFLEVGEAAQEGARRET
                     EEETELRIEVGRLIGVYSRPYAAVVTIIYEATVVGGRPEPAAETTEVRGFSPDEIPWD
                     ELAFTTTESALRDWVASLPGRGPRFEPELYVTGDES"
BASE COUNT          369 a          981 c          924 g          379 t
ORIGIN      
        1 ccgcgtcgcc ctctcgaact cgatgggcct gggcggccac aacagcacgg tgatcctgcg
       61 ccgcttcgac gagtagggcc gagcgcccac cgaggggaga gccgccacca tgacgaccac
      121 ccgagcatcc cgagaccccg gccgccgcgc cgtcgtcacc ggcctgggcg ccatcacgcc
      181 catcggcaac gacgtcgcca ccttctggca gaacctgacc gatggcgtct ccggcgtcgc
      241 cgagatcgcc tccttcgacg cctccgccct cgaggtgcgg atcgccgcgg aggtcaaggg
      301 cttcgagccg aaggactgga tggacttcaa gcaggcgcgt cgcatgagcc gattcgcgca
      361 gctggcggtc gccgctgcgc gccaggcgat cgacgattcc ggactgcaga tcggcgacca
      421 caaccgcgac gacgtggcgg tggtgatcaa caccggcggc ggcgggctgc aggaggtcgc
      481 cgtgggcgaa acgacctacc tcgagcgggg accggatcgg gtcagcccgt tcatggtgcc
      541 gatgatgtcg ccgtccatgg ccgcctgcca ggtgagcatc cagaacggca tccgcgggcc
      601 ggtcatcacc agcgtggcgg cctgcgcctc cggcgtgcag gcgttcgtcg aggcgcagcg
      661 cctgatcgag cgcggcgagg tcgacgtcgt catcgccggc ggcacggaga gcgcgatcat
      721 gccggtcagc ttcgccgccc tcgccaacat gggcgcgctc tcgaagcgca acgacgaccc
      781 gacccgcgcc tcgcgaccct tcgacgccaa ccgtgacggc ttcgtcttcg gggaggccgc
      841 cggcgtggtc gtggtcgaat ccgccgcgca cgcggccgcc cggggtgcga acgtgatcgc
      901 cgaggtggcc ggcggtgcgc ttacgggcga tgcgttccac atctcggctc ccgatcccgc
      961 ggggttcggt gccacgctcg ccatgcggcg ggcattgcgc gacagcgggc tcgagccgtc
     1021 ggaggtcgac cacgtcgtcg cgcacggcac ctcgacgccg ctgaacgacg cgacggagac
     1081 caaggccatc cacgccgcct tcggtgacga cgccgcgcgg ctggccatca gcagcaacaa
     1141 gagcatgctc ggccacacgc tcggcgcggc cggcgccgtc tcggggctgc aggccatcct
     1201 ggccatccgc gacggtgtcg tgccgccgac gatcaacctc gagacacccg acccggcctg
     1261 cgacctcgac tacatcccga acgtcgcgcg ccgcatgccg gtgaacgtgg cgatgatcaa
     1321 cggcttcggg ttcggcggcc agaacgcggt cgccgtgttc cgccgcttcc aggcgtagcg
     1381 accggcgcgc gtaccccacg cccccggact tcggtccggt tcgtcctcga ggcgtccccc
     1441 catagcatcg ggcgcgtgac cgaagtgaac ggtctccgcc tggcctaccg cgggttcgtg
     1501 gccggcctgg cgggagccta cgtctggatc gcggtggcca tgctgctcag cctcccgacc
     1561 ggcggcccga tggcgccggt gcaggcgctg gggagcatcg gtccggaagg cgcgcgcacc
     1621 tcgacgagcg aggcgctcgt gctcggcctg gggctggtcc aggtggccgg cgccggcatc
     1681 ggcatggcct tcgcctactt cttcggtcgc ttcttcacgg tgcgggccac gctgggaacg
     1741 gccgccgtgt gcgtcgccat cctcgcctgg agcctgctcg gcagccggat cgggatcgcc
     1801 atcggcgtga acgaagtcga cctggtcacc tcactggcgc tgctcgccgc gaccatcggt
     1861 tacggctggg tcgtcggcgc ctgggtgccg gtccgcggcg aggtgctgcg ctacgactcg
     1921 tcgcccgtga cgtagagctc cggctcgaac cgcggcccgc gtccgggcag cgacgccacc
     1981 cagtcccgca gcgccgactc ggtcgtcgtg aaggccagct cgtcccacgg gatctcgtcg
     2041 ggtgagaagc cgcgcacctc ggtcgtctcg gcggcgggct ccggccggcc gccgaccacg
     2101 gtggcttcgt agatgatcgt cacgacggcg gcgtacggac gcgagtagac gccgatgagc
     2161 cgcccgacct cgatccgcag ctcggtctcc tcctccgtct cgcgacgcgc cccttcctgc
     2221 gcggcctccc cgacctccag gaagccgccc gggtagctcc agcgtccgag ggccggctcg
     2281 atcccccggc gcgccagaaa gacgcggccg ccgtcgacgg gcagcgtccc gacgacgatc
     2341 cgcgggttgc gccacgtgac gtgcccgttc gggcagacca ggcgcggacg atggtgcgcc
     2401 tcgagcactc gcggctccac gcgctcgccg caggccggac agaactcggg ctcctcgccc
     2461 agggtgacgg gatacgtgtt cgaggcgtcg cgctccggca tcggtccatc ctagtgggcg
     2521 ggtggtggtc gcggcccgcc cgatgtccgg acatccgatc gaaggcctca ccaccgcatc
     2581 ggtacaatgc ggctcccatg gcgaacgacc ttccgcaggt gatgctgcac aactcgctga
     2641 cgaggcggct gga
//
LOCUS       DASTYP010000193         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_52019, whole genome shotgun sequence.
ACCESSION   DASTYP010000193 DASTYP010000000
VERSION     DASTYP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52019"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFV59_08650"
     CDS             <1..441
                     /locus_tag="VFV59_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325934.1"
                     /translation="EKTFRVTSGAAMLRSRQALLAEIAKCDIVCANCHRLRTQRRHAE
                     RRPASRGSSVYLARKRAAWRAHAELLNRLRHVPCADCARSFPPCAMDFDHRRGVTKRV
                     AVTRMIGRAGVEAILEEVAKCDIVCANCHRLRTFTRRAGIATRE"
     gene            432..507
                     /locus_tag="VFV59_08655"
     tRNA            432..507
                     /locus_tag="VFV59_08655"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:465..467,aa:Gly,seq:tcc)
     gene            857..1285
                     /locus_tag="VFV59_08660"
     CDS             857..1285
                     /locus_tag="VFV59_08660"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEU5325935.1"
                     /translation="MVLFFTCTGGFDPQQPSGGWAARRVAVDATLEERMTAAMLGLLA
                     GPTEEERDAGLTSWFSSDTAGMLNDVSIEDGLATIDFADFSRIIPNASTSAGSAMLTS
                     ELSTTLYQFAEIGGFVVQFDGSCDAFWQWLQGGCQVITRG"
     gene            complement(1292..1615)
                     /locus_tag="VFV59_08665"
     CDS             complement(1292..1615)
                     /locus_tag="VFV59_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325936.1"
                     /translation="MIVLVLAALGAAAWYLLLRPGVEVASSVDPDVTITCPASASVSE
                     EGCRDWGDAVLAAGPPSHTFEMDDLARLSLVRGPFGGACEVSYFLQRYADDPAWTDAV
                     ACPSR"
     gene            1697..>2913
                     /gene="maeA"
                     /locus_tag="VFV59_08670"
     CDS             1697..>2913
                     /gene="maeA"
                     /locus_tag="VFV59_08670"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HEU5325937.1"
                     /translation="MRPLQPPFDDLAAGRPTTIDLAGATLLSNRLFNKDTAFTLDERE
                     AFELHGLLPDHVMDIEAQVQLEMEHFHRKTDPLERYIGMAALQDRNATLFYRMLAENL
                     EELLPIVYTPTVGLACQSYSHIVRRTRGAWITPRDCDRIPDVLRNTPYEDVRLIVVTD
                     NERILGLGDLGAGGMAIPIGKLALYTGACGIYPGLTLPVSLDVGTDNPTLLEDPLYIG
                     YRAPRLRGAEYDALVDRFVQGVQEVWPGCIIQWEDFKHHNALRILDRYRDRVPSFNDD
                     VQGTSAVVVAGTLAAVRHLGRPLAAHRILLVGAGAAGIGIARLLRAAMREEGMTDDEI
                     VRSLVLVDSRGLVHAGRTDLDPTKTDFALPSELTQACGIDGEKATDLVRIIECLEPTV
                     LVGTTGTAGTFHEG"
BASE COUNT          449 a         1050 c          997 g          417 t
ORIGIN      
        1 gagaagacgt tccgcgtcac gtcaggggcg gcgatgttgc ggagccgaca agcgctgctg
       61 gccgagattg ccaagtgcga catcgtctgc gcgaactgtc accggctccg gacgcaacga
      121 cgacacgctg aacgacgtcc ggcatcccgc ggctcgtccg tctacctggc ccggaagcga
      181 gccgcgtggc gagcgcacgc tgaactcctg aatcgcctgc gccacgtgcc ctgcgcggac
      241 tgcgctcgct cgtttccacc ttgtgcgatg gacttcgacc accggcgcgg cgtgacgaag
      301 cgggtcgcag tgacgcggat gattggtcgt gccggagtcg aggcgatctt ggaggaggtc
      361 gccaaatgcg atatcgtctg cgcgaactgc caccgcctgc gaacgttcac caggcgggcc
      421 ggcatagcga cgcgggagta gctcagctgg tagagcgcga cccttccaag gtcgacgtcg
      481 cgggttcgat ccccgtctcc cgctccacct caaccgtcgc tgcctcggga cgctccgaac
      541 gggccggggg caggtgacct tcggccctca tcggccgccg gctggctccg tagcgtcgtc
      601 atcggagggc cgatgcatgt cgccacggac acggacggga gggctcgccg ctgtggtgac
      661 gctgctgctt gtcgcgtgcg ggggtgtctc ctcgccctcg gccacccgca gctccgtgcc
      721 gtcgcgtgcg accccaacct cgagcgcgac cacgagagcg agcgcgtcgg acacggagcc
      781 agccgccatc atcccgtcgg ccgaaccgac cggatgcggc tggggcccgg cctcgcccac
      841 acccggaccg gacgcggtgg tcctgttctt cacctgcacg ggcggcttcg acccgcagca
      901 gcccagcggc gggtgggcgg cacgccgggt cgccgttgac gcgacgctcg aggagcggat
      961 gaccgccgcc atgctcgggc tcctcgccgg cccaacggag gaggagcgcg atgccgggct
     1021 cacgtcgtgg ttctcgtcgg acacggcggg gatgctgaac gacgtcagca tcgaggatgg
     1081 cctggcgacc atcgacttcg cggacttctc acgcatcatc ccgaacgcgt cgacatccgc
     1141 tggctcggcg atgctcacga gcgaactctc gaccactctg taccagttcg cggagatcgg
     1201 ggggttcgtc gtccagttcg acggcagctg cgatgcgttc tggcagtggc tccagggcgg
     1261 ctgccaggtg atcacccgtg gctagggggc gtcagcgcga gggacaggcc accgcatcgg
     1321 tccaggccgg gtcgtccgcg taccgctgca ggaagtacga cacctcgcac gccccgccga
     1381 acgggccacg caccagcgac agccgggcca ggtcgtccat ctcgaacgtg tgcgacggcg
     1441 gaccggccgc cagcaccgca tcgccccagt cgcgacaccc ctcctccgag accgatgcgg
     1501 acgccgggca ggtgatcgtc acgtccgggt cgacggacga ggcgacctcc acgccgggcc
     1561 gcagcaacag gtaccaggcg gccgcgccga gggccgccag aacgaggacg atcatggcca
     1621 tcattcgacc catcgcgcgg catcatgccc gatcgccacg ccggcgcgcg gccggcgccc
     1681 ggtatgctcg aagctgatgc gccccctcca gccccccttc gacgatctcg cggcgggacg
     1741 gccgacgacc atcgacctcg ccggcgccac gctgctgagc aaccgcctct tcaacaagga
     1801 caccgccttc acgctcgacg agcgcgaggc gttcgagctc cacggcctgc tgccggacca
     1861 cgtcatggac atcgaggcgc aggtccagct cgagatggag cacttccacc gcaagaccga
     1921 tccgctggag cggtacatcg gcatggcggc cctccaggac cggaacgcga cgctcttcta
     1981 ccgcatgctc gctgagaacc tcgaggagct gctgccgatc gtctacacgc ccaccgtcgg
     2041 cctcgcctgc cagtcgtact cgcacatcgt gcggcggacg cgcggggcgt ggatcacgcc
     2101 gcgcgattgc gaccgcatcc ccgacgtgct gcgcaacacg ccgtacgagg atgtgcggct
     2161 catcgtggtg accgacaacg agcggatcct ggggctgggc gacctcggtg ccggcgggat
     2221 ggcgatcccc atcggcaagc tggccctcta caccggcgcc tgcggcatct atccgggcct
     2281 cacgctgccg gtgagccttg acgtcgggac cgacaacccg acgctgctcg aggacccgct
     2341 gtacatcggc taccgcgcgc cgcggctgcg cggcgccgag tacgacgcgc tggtggatcg
     2401 gttcgtccag ggcgtccagg aggtctggcc gggctgcatc atccagtggg aggacttcaa
     2461 gcaccacaac gccctgcgca tcctggaccg ataccgcgac cgcgtcccct ccttcaacga
     2521 cgacgtccag ggcacgtcgg ccgtggtggt ggccggcacg ctggccgcgg tgcgccacct
     2581 gggccggccg ctggctgcgc accgcatcct gctggtcggt gccggcgccg cggggatcgg
     2641 gatcgcccgg ctgctgcgcg ccgcgatgcg cgaggagggg atgacggatg acgagatcgt
     2701 ccgctccctc gtgctggtcg actcgcgcgg gctcgtccac gccggccgca cggacctgga
     2761 tcccaccaag accgacttcg cgctgccgtc ggaactgacg caggcctgcg ggatcgacgg
     2821 ggagaaggcg acggacctgg tgcggatcat cgagtgcctg gagccgacag tcctggtggg
     2881 caccaccggc accgccggga ccttccacga ggg
//
LOCUS       DASTYP010000194         5400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_235189, whole genome shotgun sequence.
ACCESSION   DASTYP010000194 DASTYP010000000
VERSION     DASTYP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235189"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFV59_08675"
     CDS             complement(<1..261)
                     /locus_tag="VFV59_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325938.1"
                     /translation="MSSWFDRLLEELQRRQAEADARREGRPFEPRRGAEPRNVTPPPT
                     EVRRRTNGGDRTPPPTGRPPFPTAAGGGGEELPWRRWLLIGGL"
     gene            complement(729..2288)
                     /gene="guaA"
                     /locus_tag="VFV59_08680"
     CDS             complement(729..2288)
                     /gene="guaA"
                     /locus_tag="VFV59_08680"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5325939.1"
                     /translation="MDTEAPEQPDTVAVLDFGSQFAQLIARRVRELNVYSELLPHDTP
                     MAELERRGIKAVILSGGPSSVYDERAPKADPALWSGRLPVLGICYGLQLMAHELGGEV
                     VPSTKREYGPATVSVTREGGLFGGIEGEQPVWMSHGDSILRPPEGFHATAQTDSTPYA
                     GLADPSRNLYGIQFHPEVVHTPRGRDLLRNFVVDIAGVRPTWTAASFIDTNVADIRER
                     VGSGSVICALSGGVDSAVAATLVHRAIGDQLTCIYVDHGLMRKRESELLRVTFERDLG
                     MRLVMVDARDRFLRRLSGVEDPEEKRRIIGDEFIRVFEEEAAKLGQIDFLVQGTLYPD
                     VIESTTAETKTAQKIKTHHNVGGLPPDLRFRLIEPLRYLFKDEVRRVGTELGLPEAMV
                     QRQPFPGPGLAIRIIGEVTAERLDTLREADWIVIDEIKAAGLYRSLWQSFAILTPVRS
                     VGVMGDGRTYANVVAVRAVTSDDGMTADWARLPYEVLARISSRIVNEVPGVNRVVYDV
                     SSKPPATIEWE"
     gene            2515..3000
                     /locus_tag="VFV59_08685"
     CDS             2515..3000
                     /locus_tag="VFV59_08685"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU5325940.1"
                     /translation="MIDVQIREPMPTPTVAVRVQQPMSELDIGALFDEYLPNVAHHLA
                     DVGVEPAGPPYARYHEFGPERVDVEFGFPVDLPVSNVPDLADARPGEIASSVLPGGRL
                     AVAVHHGAYDGLPAVYERVQAWIREQGLSSGGPPWESYVDDPSEVAEADLRTEVCWPI
                     G"
     gene            complement(2997..3812)
                     /locus_tag="VFV59_08690"
     CDS             complement(2997..3812)
                     /locus_tag="VFV59_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU5325941.1"
                     /translation="MGSSDWSARRDRFRQLHEHGLFVMPNPWDIGSARILASMGFPAL
                     ATTSLGHAASLGKVDQTVTRDELVTHVAAISAAVDLPLNVDAERCFADDLAGVAETVG
                     LLAQAGASGCSIEDYDPARALIDTIAVAADRVAAAAEAAHREGMLLTARAENALYGID
                     DIEDTVTRLRAYREAGADVVYAPRLTDAGDIRRVVAATDAPVNVLAMRHGPSIPELAE
                     LGVRRVSTGGPLARAAYGALRRAAEELLGPGTSTYQDDALTSDDLERAFGTGH"
     gene            complement(3847..5070)
                     /locus_tag="VFV59_08695"
     CDS             complement(3847..5070)
                     /locus_tag="VFV59_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5325942.1"
                     /translation="MSPKRASAYRRPIYEEILERAHEPRGFIQVLAGPRQVGKTTLAR
                     QVMDAIGIPGHFASADAPDPEDVGWIYTQWQIGRQAARNGGRAGGLLVLDEVQKIRAW
                     SDVVKQLWDEDTASGVPLRVMLLGSAPLLVRQGLSDSLAGRFEVIRVTHWTFAEMRDA
                     FGWDLDTYLFYGGYPGAAALIKRHDRWEQYILDSIVETNLSRDILMLNRVEKPALLRQ
                     LFRLACSYSGQVLSYTKMLGTLTDAGNTVTLAHYLELLGGAGMVTGVGKYSGSVVRRR
                     ASSPKLLALNTALISATARTTFAQARADHEFWGRLVETAVGAHLWAHADPEELTYWRE
                     GNDEVDWVIRPGKLLRGRDEPVAIEVKTGHARGLPGTVAFTQTHRGSTALVVGAGGID
                     PEAFLSTHPREWLSV"
     gene            complement(5115..>5400)
                     /locus_tag="VFV59_08700"
     CDS             complement(5115..>5400)
                     /locus_tag="VFV59_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5325943.1"
                     /translation="NAPAPAYGDAFPIAWAETGRRLLSLVDGAVVPGHGDPFDVHFAE
                     RQVAEIAVVAELARDAVAGSIGLDEAIRRSPFPAEVTAEAIERARLEVGE"
BASE COUNT          759 a         1978 c         1864 g          799 t
ORIGIN      
        1 cagcccgccg atcagcagcc atcgacgcca gggaagctcc tcgccgccgc cgccggctgc
       61 ggtggggaag ggcggacgcc cggtgggggg cggggttcgg tcgccgccgt tcgtccggcg
      121 acgcacctcg gtcgggggcg gcgtcacgtt gcgcggctca gcaccacgcc gaggctcgaa
      181 cgggcgtcct tcgcgccgcg cgtcggcctc tgcctgtcgg cgctgcagct cctcgaggag
      241 cctgtcgaac cagctggaca tctgctgcgg gaatgctacc cgcttggccg ctcggcgtcc
      301 tccggcgcat cggttccgag ccagtcccgc ccgacgggac tatcgggcac ttcttccccg
      361 atgcggcccg cgcacgcaac gatcgacaac cttcagcctc gattcggccc cgagcctccc
      421 catccctgcc gccccgcgac acatccgacg actccctgcc cggtactccc ggcccacgca
      481 aaccggcgag gaacggcagc cgggacgtgc tcgagttgtc agccgtctgt cgtgacgacg
      541 ttaggcggct tccgcgcgac ccgagtcgtc agccgtctgt cgtggcggcg ttacgcgcgc
      601 cacgattagg tcgatgagcg tgagtcgcca cggtcgcccc gtggtcggtg cccggcaggc
      661 cgtctaccgt cgccaggatg cgcatgtggc aacttggcgc tcgcggcggg atccgtgcat
      721 ctgccgcgtc actcccactc gatggtcgcg ggcggcttcg agctgacgtc gtagacgacg
      781 cggttgaccc ccggcacctc gttcacgatc cgcgacgaga tgcgcgccag cacctcgtat
      841 ggcagccgcg cccagtcggc cgtcatcccg tcatccgacg tgacggcccg caccgccacc
      901 acgttcgcgt aggtccgccc gtcgcccatc acaccgaccg accgcaccgg cgtgaggatc
      961 gcgaacgact gccacagcga ccgatacagg cccgcggcct tgatctcgtc gatcacgatc
     1021 cagtcggcct cgcgcagcgt gtcgagccgc tcggccgtca cctccccgat gatccggatc
     1081 gcgagccccg gccccgggaa cggctggcgc tggaccatcg cctcgggcag gccgagctcc
     1141 gtgccgacgc ggcgcacctc gtccttgaag aggtagcgca gcggctcgat gagccggaag
     1201 cgcaggtccg gtggcagccc gccgacgttg tggtgggtct tgatcttctg cgccgtcttc
     1261 gtctcggcgg tggtcgactc gatgacatcg gggtagagcg tcccctgcac caggaagtcg
     1321 atctgcccga gcttcgcggc ctcctcctcg aagaccctga tgaactcgtc gccgatgatc
     1381 cgccgcttct cctcgggatc ctccacgccc gacagccggc ggaggaaccg atcccgagcg
     1441 tcgaccatca ccagccgcat gcccaggtcc cgctcgaagg tgacccgcag cagctccgac
     1501 tcgcgcttgc gcatcagccc gtggtcgacg tagatgcagg tcagctggtc gccgatggcc
     1561 cgatgcacca gggtcgcggc cacggccgag tcgacgccgc cggagagcgc gcagatgacg
     1621 ctccccgacc ccacccgctc tcggatgtcg gccacgttcg tgtcgatgaa gctggccgct
     1681 gtccaggtcg gccggacccc tgcgatgtcg accacgaagt tgcgcagcag gtcgcggccg
     1741 cgcggcgtgt gcacgacctc ggggtggaac tggatgccgt agaggttgcg ggacggatcg
     1801 gccaggccgg cgtatggggt cgaatcagtc tgggccgtgg cgtggaagcc ctccggcggc
     1861 cggaggatcg agtcgccatg gctcatccag accggctgct caccctcgat gccgccgaac
     1921 aacccgccct cgcgcgtcac gctgaccgtc gcgggaccgt actcgcgctt cgtcgacgga
     1981 accacctcgc cacccagctc gtgggccatc agctgcaggc cgtagcagat gccgaggacc
     2041 ggcaggcggc cggaccagag ggcgggatcg gccttcgggg cacgctcgtc gtagaccgac
     2101 gacggaccac ccgacaggat gacggccttg atgccgcgcc gctccagctc ggccatcggc
     2161 gtgtcgtgcg gcaggagctc ggagtagacg ttgagctcgc gcacgcgccg agcgatcagt
     2221 tgggcgaact ggctgccgaa gtccaggacg gccacggtgt cgggctgctc cggcgcctcg
     2281 gtgtcgatcc gctcgggtgg cggcgcgtgg agcgcctgct ccaggtacgc ctcgacctcg
     2341 gcgtcgtccg gggcgaggac gcggtgcccg gcgttggccg cctcgccgtg caccgtgcgg
     2401 ccgcgatcgg ccggttgtga cgttggactc tcgctcaatg ccgctccgtt cggcgcagga
     2461 tgatgtcgtc cgagtctacc gcccggcacg ccgcccgcag gaggtccgaa caccatgatc
     2521 gacgtccaga ttcgcgagcc gatgcccacc cccaccgtcg ccgtccgcgt ccagcagccg
     2581 atgagcgagc tggacatcgg cgccctcttc gacgagtacc tcccgaacgt cgcgcaccac
     2641 ctcgccgacg tcggcgtcga gccggccggc ccgccctacg cccgctacca cgagttcggc
     2701 cccgagcgcg tggacgtcga gttcggcttc ccggttgacc tcccggtctc gaacgtcccc
     2761 gacctggccg atgcgcgccc gggcgagatc gcctcctccg tgctgcccgg cgggcgactg
     2821 gctgttgcgg tccatcacgg cgcgtacgac gggctcccgg ccgtctacga gcgtgtccag
     2881 gcgtggatcc gggagcaggg gctgtcgtcc ggcgggccgc cctgggagtc ctacgtcgac
     2941 gatccgtcgg aggtggccga ggccgacctg cgcaccgagg tctgctggcc gatcggctag
     3001 tgcccggtgc cgaaggcgcg ctccaggtcg tcgctcgtga gcgcgtcgtc ctggtacgtg
     3061 gaggtgcccg gaccgagcag ctcctccgcc gcgcgccgca gggctccata cgccgcgcgc
     3121 gccagtgggc caccggtgga cacgcgccgc acgccgagct cggccagctc ggggatcgac
     3181 ggtccgtggc gcatggccag cacgttgacc ggcgcgtcgg tcgccgcgac cacgcgccgg
     3241 atgtcgccgg catcggtcag gcgaggcgcg tagacgacgt cggcaccggc ctcgcggtat
     3301 gcgcggagcc tcgtcacggt gtcctcgatg tcgtcgatcc cgtacagggc gttctcggca
     3361 cgcgccgtga ggagcatgcc ctcccgatgc gcggcctccg ccgccgcggc gacgcgatct
     3421 gctgccaccg cgatcgtgtc gatgagcgca cgggccgggt cgtagtcttc gatcgaacag
     3481 cctgaggcgc ccgcctgggc cagcaggccg accgtctccg cgacgccggc aaggtcgtcc
     3541 gcgaagcacc gctcggcgtc gacgttcagg gggaggtcga cggccgcgga gatcgccgcc
     3601 acgtgggtca ccagctcgtc gcgggtgacc gtctggtcca ccttgccgag ggaggcagca
     3661 tggccgaggc tcgtcgtagc cagcgcgggg aagcccatcg aggccaggat ccgggcggag
     3721 ccgatgtccc acgggttcgg catgacgaac aggccgtgct cgtggagctg acgaaaccga
     3781 tcgcggcgtg cggaccagtc ggatgagccc atcggcatca gcgtaccgcg agttcggcac
     3841 acgccgctag acgctcaacc actcgcgcgg gtgggtcgac aggaaggcct ccggatcgat
     3901 ccccccggcg cccaccacga gcgcggtcga gccgcggtgc gtctgggtga aggcgaccgt
     3961 cccggggagg ccgcgagcgt ggccggtctt gacctcgatc gccacgggct cgtcgcgccc
     4021 gcgcaggagc ttccccgggc ggatgaccca atcgacttcg tcgttgccct cgcgccagta
     4081 ggtcagctcc tccgggtcgg cgtgcgccca gagatgcgcg ccgacggcgg tctcgaccag
     4141 ccggccccag aactcgtggt cggcgcgagc ctgcgcgaag gtggtgcgcg ccgtggcgga
     4201 gatgagcgcc gtgttgaggg ccagcagctt cggcgacgat gcccggcgcc gcaccaccga
     4261 gcctgagtac ttccccaccc cggtcaccat cccggcgccg ccaaggagct cgagatagtg
     4321 ggcgagggtc accgtgttgc ccgcgtcggt cagggtgccg agcatcttcg tgtagctcag
     4381 gacctggccg gagtacgagc acgccagccg gaagagctgg cgcagcagcg ccggcttctc
     4441 cacccggttg agcatgagga tgtcgcgcga caggttcgtc tccacgatcg agtcgaggat
     4501 gtactgctcc caccggtcgt ggcgcttgat gagcgccgcg gcaccggggt agccgccgta
     4561 gaagaggtag gtgtcgaggt cccagccgaa ggcgtcgcgc atctcggcga aggtccagtg
     4621 cgtgacccgg atgacctcga accggccggc cagtgagtcc gacagcccct gccgcaccag
     4681 cagcggcgcg gacccgagca gcatcacgcg caacggcacc cccgacgcgg tgtcctcgtc
     4741 ccacagctgc ttcacgacgt cggaccaggc gcggatcttc tgcacctcgt cgaggacgag
     4801 cagcccaccg gcacgccccc cgttgcgcgc cgcctgccgc ccgatctgcc actgggtgta
     4861 gatccagccg acgtcctccg ggtcgggcgc atccgccgag gcgaagtgcc ccgggatgcc
     4921 gatggcgtcc atgacctgcc gcgccagcgt ggtcttgccc acctgtcggg gcccggccag
     4981 gacctggatg aagccgcgag gttcgtgggc ccgctccagg atctcttcgt agatcggtcg
     5041 ccggtacgcg ctggcgcgct tcgggctcat ctgctcgctc cgctgagtgg atttactcag
     5101 tgacgcgagt aactttactc accgacctcg agacgcgcgc gctcgatcgc ctcggcggtc
     5161 acttcggccg ggaacggcga gcgccggatc gcctcgtcca ggccgatgct gcccgccacc
     5221 gcgtcgcggg ccagctctgc caccaccgcg atctcggcga cctggcgctc ggcgaagtgg
     5281 acgtcgaacg gatcgccgtg accgggaacc accgccccgt cgaccaacga cagcagccgt
     5341 cggccggtct ccgcccaggc gatcgggaac gcgtcgccgt aggccggggc cggcgcattc
//
LOCUS       DASTYP010000195        13976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_380814, whole genome shotgun sequence.
ACCESSION   DASTYP010000195 DASTYP010000000
VERSION     DASTYP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380814"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2532
                     /locus_tag="VFV59_08705"
     CDS             <1..2532
                     /locus_tag="VFV59_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5325944.1"
                     /translation="ALAVWRRLGRDAANPPTEPIRPPRAAEAAPTTPDAAEAPLVATE
                     PTAPVAPAPRPPGRTLPADLEPHPTGIFAAVGRFDYRFRKVLPVIGLLIVAGLLVWSR
                     VDGGDLIQGGWVIDGSEEQQAEALLADRFGEPSTSMIVLFTDPEGDAASSAFQDRVAE
                     TLDPIRDDPLVTGITTYADSPVEQLLSRDGSKTLAIVTLNEELEASVDQAFELGQRIE
                     PPSGVEAEVTGIPQLYHEFNQKIEEDLVQAEMISLPIALVILLAVFGTLVGAVLPLLI
                     AAFALPAAFAVIGLLAGVTDMSIFVTNLASMIGLALAIDYSLFMVSRYREELRHHSVE
                     IAIERMMGSVGKAVAVSGVAVAIGLSSLTVFEAAALRSMGIGGIITVVSTLLFALTVL
                     PAILAMLGSRVNRLRIPLPGRLRLAEEDADAADVRQGHGLWGWIAGHVMRRPVLISLP
                     VLALLLLAGSPFLGIQLSTGQNLEDLPSTPARTAFMTITEDFPGGESDPIRVALTWED
                     ATPGEDGLSVEQQSAMQSYVDDLGALGDVTGVESVLDAPPGIPAEQYRQLLALPPSQR
                     PAEVRDALDEYVSEWLAGDTAEIQVRSRLLPDSDAGRALVDEVRAVDTPDETDALTAG
                     LPSRSRDFMDSFYDSVPVAVLIVVAVTGGILFLTFGSVFLPVKAVLMSLVSVSASFGA
                     LVWIFQEGHLSAILGVEPSGTTAAWLPIIMFAILFGLSMDYEVLLLSRIRERYLAHGD
                     NTRAVREGIGITGGIITGAALIMVAVFGAFSLSSIIFLKALGFSMALAVLVDATVVRG
                     ILVPAFMRLAGFVNWWAPRWIQRIVAKLGLYEGPDVEAAPAGSVP"
     gene            3056..3337
                     /locus_tag="VFV59_08710"
     CDS             3056..3337
                     /locus_tag="VFV59_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325945.1"
                     /translation="MTRDDRLQALTERWRARHDARRPSPERRQSAPSEREALAKRAFP
                     YRSTPPAEYVDAHGADMTAFTYDDERYADPDLDAWILEVGRLLRERSGR"
     gene            3334..3939
                     /locus_tag="VFV59_08715"
     CDS             3334..3939
                     /locus_tag="VFV59_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325946.1"
                     /translation="MSAPPWAAALVHQVCADAGVEPPRLLWRRRKDEHSTGVARRSEG
                     TIAVRAGRDPMDQRLTLLHELAHWLSPPARRRRGSRPAEHHGLAFYRIAFELYRRHGI
                     PAADAVRLESARYRSSLRHAAALGIDGAVDALRAHRATLRERGRREWRVLVPEHPIRL
                     ARDGRWSVCETCGQRVVGSNLARVRRARRPVRHVLLGAVAS"
     gene            complement(3936..4844)
                     /locus_tag="VFV59_08720"
     CDS             complement(3936..4844)
                     /locus_tag="VFV59_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5325947.1"
                     /translation="MKIGLMLPLGADETSGFADLRAMAIAAEESGLDSVWGADHLIFR
                     DEGETVGIHECWTVLTAVAAVTSRVEIGPLVLALPFRNPALVAKMAAELDEVSDGRLI
                     LGLGCGWHKPEFDAFGYPFDHRVSRFAEGMEIIAPLLRGETVTFEGRFHRAQDLVLLP
                     PPVRAGGPPILIAGKQPRMLELVAKHAAQWNAAWYGDPRTATDLHERLSNLDGALEAA
                     GRDPSTLERTAGIFVAFEGADEDTPERAIRGTTEEMADVLARYAEYGVTHLIAHIFPR
                     TTDAVARLGEAAALARERAGLVAQAS"
     gene            4895..6031
                     /locus_tag="VFV59_08725"
     CDS             4895..6031
                     /locus_tag="VFV59_08725"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5325948.1"
                     /translation="MTDPPPRLPLDGIRVIDVSRVLAGPFATMLLADLGADVVKIEPP
                     EGDETRTWGPPWWGDPADRRSAYFASVNRNKRSVVLDLRTAEGQARLHRLLAGADLLV
                     HNYRPATAARLGLETDALRARHPRLVVAHVGGFPAPDADRPAYDLLAQAVSGLMSVTG
                     TPNGPPLKVGVALLDLIAGLECAVGALAALLGRGRVREVEVGLVEAGVTSLANVLANH
                     LATGDQPPRHGNAHPNIVPYQAFAARDGDLVIAVGNDAQFARLLNVLGLVDADARFAT
                     NPRRLAARDELITRLTDAIAERGRAELLAALEAADVPAGPVNTVGEAVAAMGAGWVHQ
                     VDGIRLAPDPIRVNGAALPVRMPPPLLGQHTAELLEDASASAER"
     gene            6069..6362
                     /locus_tag="VFV59_08730"
     CDS             6069..6362
                     /locus_tag="VFV59_08730"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HEU5325949.1"
                     /translation="MDESPATGDQAASFSLDEWIERLRTALGSADPGIELGPEEREAI
                     LELTRVAAHASQRVAAPFTAFLVGAALDDLAEPARIERLRALTSMLSRAGTGE"
     gene            complement(6366..7625)
                     /gene="nrfD"
                     /locus_tag="VFV59_08735"
     CDS             complement(6366..7625)
                     /gene="nrfD"
                     /locus_tag="VFV59_08735"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5325950.1"
                     /translation="MTAHAEPRAPAGAQRRVGGFGLWLGLLALLMLVGFGAWVYQVSN
                     GLAVTNLRNTVMWGQYILFFMFFVGLSAGGLIVASSGRLFGATRFGPIVRLAVLEATV
                     AVMLAAIFLFPDIGRPERLWHLFRYPNLTSPMIWDIAIVTVYFALSALYVWLYTRADL
                     AAAGSRLALGTSASVEARARDDRLKGALAWVALPAAVLLHSITAWIFGLQISRGFWYT
                     AIMAPMFVASALVSGLALVILLALVVRRVGRLRFDDGLVAYLGGLLAVFISVEAFFVL
                     SEALTAAYPGAEFEGDPIARMLTGQFAPLFWFEVGVGLLVPFVMLARRGWRASPRVVA
                     IAAGIAIVGIYVHRLNLVLNGLAVSPIGLPPGVSIGTPQDGASFAMSNWYVPSAIEWL
                     IVIGVLAFGAFVFTMAARWLPLEPTDH"
     gene            complement(7622..8329)
                     /locus_tag="VFV59_08740"
     CDS             complement(7622..8329)
                     /locus_tag="VFV59_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5325951.1"
                     /translation="MPDRRSPVDLLSPASRAYGAIEPPSSSPRWGMVVDTERCIGCWS
                     CAVVCKSENNVPLGMWWNRILTEGEGLDLPTTEHGREEMSWTPLACQHCDNAPCVKVC
                     PVQATYRREDGVVMMDSARCIGCRYCMIACPYGVRTFNWGAPARPNGLETGMVEARPV
                     GTVEKCTLCVHRLEEGQVPSCVWSCPAGARIFGDLDDPDSRVSRLVRDRQGEMLLEEK
                     GTLPKVRYLPPRRRREL"
     gene            complement(8322..10850)
                     /locus_tag="VFV59_08745"
     CDS             complement(8322..10850)
                     /locus_tag="VFV59_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5325952.1"
                     /translation="MTVSRRRFLKGAAAVGATTAAAAGGAGFVLLGRDHPAVAASEGV
                     DRVVRTTCSPNCTGSCGQLAFVRDGKVVKVQQAADYPDTAYNPRGCMKGISYHTLIHG
                     RDRILTPLIRTGERGSGEFRRATWDEVLDRIAGDMKRIGERWGYDSIHVFGQVPGSGY
                     IQKGANYRASALLGLTHGTSFDFNGDLPMGMPITFGVQNAEHEAKDWANSRFLLLIGA
                     NPVETRIPDVHFLFDALERGAQMVVVDPNFSGTASKADTWLQIRPGTDAALGLGLARE
                     IVVRELADFDFLRTYTDAPLLVRLDTGVRLREADVVAGGSDARFAIWDEAAGQPAWVG
                     TERLGMPDGTRAAVSGTYRVRLADGSAVQVAPGFELVWRELERWTGDRTAEVTGISAE
                     MIGRLAHAYAATKPAAILMGGGANHWFHGDLTGRAMGLLASMTGNIGRSGGGFSVYVG
                     QYKVRVNVGPWNNPDGVKAHIVPSIYFVRGRSDTMHPDVPYPANGFHGLVVTFANMFV
                     QAPDVNRLHQTLAGLDLVVVVDHQMTETVRWADVVLPAATWYEKTDLTATPLHPFLQL
                     QQAAIDPVGESRTELWMWRELVRRIAPDQAAAMDVSEDEAIEMILAAGADGPTKGITL
                     ADLRAGPVRLNVPDPDIPFLKQIEDLEPFPPVSLPAPIEATAAFLPTRRIELYKEEQR
                     FRDLGETVPTYRDPHDDGVHDPAVYPLTFLTPHSKWRIHSTYGNNPWLAEIHGGRPKL
                     LIHPDDASRRGIEPGDLVEVYNTRGAFRAWAEVSEAAGIGSVTLPEGWWPRDFAAGKG
                     VNELTTSEVNPIHEVHFVANMWSPSTGWKDCRCDVRRIAGSSDA"
     gene            complement(10847..11086)
                     /locus_tag="VFV59_08750"
     CDS             complement(10847..11086)
                     /locus_tag="VFV59_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325953.1"
                     /translation="MDPLFVRALSRRRMLFAGAALVGAFGATGMARAAAAAGPSVLPT
                     LRPDATETSDARCAACGSPDHTMLSGACLPQRLRA"
     gene            complement(11255..11470)
                     /locus_tag="VFV59_08755"
     CDS             complement(11255..11470)
                     /locus_tag="VFV59_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325954.1"
                     /translation="MTVQQHAPRLEQVRAPVAISRATLIAAAVVVLVVLAIVAAWALL
                     QPTPLIDEFTVQDQQLFQLRDALGGFI"
     gene            11667..12713
                     /locus_tag="VFV59_08760"
     CDS             11667..12713
                     /locus_tag="VFV59_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5325955.1"
                     /translation="MQHAGTAGRPARLIARQRHTGRRILLSITTAAILGLAAYLVYVG
                     VEGSSRFVAAYASTTCTRPSDLPYGWTYEAVNYDSTLDAGSACAPVGARAGGEVLAAD
                     GTPIAGWYIPAAARSGDTATVVVVHGHDANKSHMLPIAAALHDRFDLLLVDLRATGYS
                     GGSQQTMGVLEQQDVRAMVDWLVETKAPPRIAALGVSGGAAATLAAMRTDERIGAFIG
                     DSMHARIGTLLAQQVDNAELWGISQPAYPGVWAAQVAIWLRTGGLDLASVDPIDSLAW
                     LGDRELLLIHGTADAEDVPAESVDANFEEARRLGIDVTRRDCTGGAHAGLATACATEY
                     GTWVSEFLDSAFAD"
     gene            complement(12710..13336)
                     /locus_tag="VFV59_08765"
     CDS             complement(12710..13336)
                     /locus_tag="VFV59_08765"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU5325956.1"
                     /translation="MTVAREPGRRHRERICGAVLVGGASLRMGRDKAGVVFGGRTLLS
                     RVAETLAEVADRVLVVARADQAVDAPSGTELVRDAIAGAGPLAGLDAALRASGSDLLI
                     VVAVDMPLVRRDVLELLIDAARADPAADAVMLVSDHGVEPLHAVYRPSATSVIASRLG
                     RGAFRLLDLIDDLEVVLIPPARWRALDPDGVSTTNVNTPEDLQRIDRR"
     gene            complement(13343..>13976)
                     /locus_tag="VFV59_08770"
     CDS             complement(13343..>13976)
                     /locus_tag="VFV59_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEU5325957.1"
                     /translation="DFALPSELTQACGIDGEKATDLVRIIECLEPTVLVGTTGTAGTF
                     HEGVIRAMADRCERPVVMPLSNPTAVTEARPGDVIGWTGGRALVATGSPFPPVTFGGE
                     THEIGQANNVFVFPGLGLGAIVAEATHITDAMVLAAARALAAAVTPERLACGALYPSV
                     RDLRSVSRSVAIAVGREAIESGHAGGSPETLEAEVDAAMWWPQYVPYQRG"
BASE COUNT         2008 a         5013 c         4993 g         1962 t
ORIGIN      
        1 gcgctggccg tgtggcggcg cctcggccgc gatgcggcca accctcccac tgagccgatc
       61 cggccgcccc gggcggccga ggcggcgccg accacgcccg atgccgccga ggcgccgctc
      121 gtggcgaccg agccgactgc gccggtcgcg ccggcgccac gcccgccggg ccgcacgctc
      181 cccgcggacc tcgagccgca tccaacgggc atcttcgccg ccgtcggacg cttcgactac
      241 cgattccgca aggttctgcc cgtcatcggc ctgctgatcg tggccgggct gttggtgtgg
      301 agccgcgtcg acggaggcga cctcatccag ggcggctggg tgatcgacgg atccgaggag
      361 cagcaggcgg aggcgctgct ggcggatcgg ttcggcgagc cctcgacctc gatgatcgtg
      421 ctgttcaccg atccggaggg tgacgcggcg tcgtccgcgt tccaggaccg ggtggccgag
      481 acgctcgatc ccatccggga cgacccgctc gtgaccggca tcaccaccta cgccgactcc
      541 ccggtcgagc agctgctcag ccgagacggg tccaagacgc tggcgatcgt gacgctcaac
      601 gaggagctgg aggcatcggt cgaccaggcg ttcgagctcg gccagcggat cgagccgccg
      661 tccggcgtgg aggcggaggt caccggcatc ccgcagctct accacgagtt caaccagaag
      721 atcgaggagg acctcgtgca ggcggagatg atcagcctcc cgatcgcgtt ggtcatcctg
      781 ctcgcggtct tcgggacgct ggtcggcgcg gtgctgcccc tgctcatcgc cgccttcgcg
      841 ctgccggccg ccttcgcggt gatcggcctg ctcgccggcg tgaccgacat gagcatcttc
      901 gtcaccaacc tggcctcgat gatcggcctg gcgctggcga tcgactactc gctcttcatg
      961 gtgagccgct accgcgagga gctgcgccac cactcggtcg agatcgcgat cgagcggatg
     1021 atgggcagcg tcggcaaggc ggtggccgtg tccggcgtgg cggtggccat cggcctctcg
     1081 tcgctcaccg tcttcgaggc cgccgcgctg cggagcatgg gcatcggcgg gatcatcacc
     1141 gtcgtctcga ccctgctctt cgccctgacc gtgctcccgg ccatcctggc catgctcggg
     1201 tcgcgcgtga accggctgcg catcccgctc cccgggcgcc tgcgcctcgc ggaggaggac
     1261 gccgatgcgg cggacgtgcg gcagggccac ggactctggg gctggatcgc cgggcacgtg
     1321 atgcggcggc cggtcctcat ctcgctgccg gtgctcgcgc tgctgctcct ggcggggtcg
     1381 ccgttcctcg gcatccagct ctcgaccggc cagaacctgg aggacctgcc gtcgacgccg
     1441 gcgcggacgg cgttcatgac catcaccgag gacttcccgg gcggcgagtc ggacccgatc
     1501 cgcgtcgcgc tcacctggga ggacgccacg ccgggcgagg acggtctgag cgtcgagcag
     1561 cagtcggcga tgcagtccta cgtggacgat ctcggcgcgc tgggcgacgt gaccggcgtc
     1621 gagagcgtgc tcgatgcccc acctggcatc ccggcggagc agtaccgtca gctgctggcg
     1681 ctgccgccgt cgcagcgccc ggccgaggtc cgcgatgcgc tcgacgagta cgtgtccgaa
     1741 tggctggccg gggacaccgc cgagatccag gtccgcagcc ggttgctgcc cgactccgat
     1801 gccggtcgcg ccctggtcga cgaggtgcgc gccgtcgaca cgccggacga gaccgatgcg
     1861 ctcaccgccg gccttccgtc ccggtcgcgc gacttcatgg acagcttcta cgactccgtg
     1921 ccggtggcgg tgctgatcgt ggtggccgtc accggcggga tcctcttcct gacgttcgga
     1981 tcggtcttcc tgcccgtcaa ggccgtgctc atgagcctgg tcagcgtctc cgcctcgttc
     2041 ggggcgctcg tatggatctt ccaggagggc cacctgtccg cgatcctggg cgtcgagccg
     2101 tccggcacga ccgcggcctg gctgccgatc atcatgttcg cgatcctctt cggcctctcc
     2161 atggactacg aggtgctgct gctctcgcgc atccgtgagc gctacctggc ccacggcgac
     2221 aacacgcgcg ccgtgcggga ggggatcggg atcacgggcg gcatcatcac cggcgccgcg
     2281 ctgatcatgg tcgccgtgtt cggcgccttc agcctgtcga gcatcatctt cctcaaggcg
     2341 ctcgggttca gcatggcgct ggcggtgctg gtcgacgcca cggtcgtgcg cggcattctg
     2401 gtgccggcct tcatgcgcct ggccgggttc gtgaactggt gggcgccgcg atggatccag
     2461 cggatcgtcg cgaagctggg gctgtacgag gggcccgacg tggaggcggc accggccggg
     2521 agcgttccgt aggggcgcgg tcagcggctg gagttgctcg gtggcggaac tagcggacac
     2581 gcaacgtcgc gaatcggccg cttcctcccc gcacattgcc gtgcggcgcg agccatggtg
     2641 acggagggag gcggaatccg cgcaacttcg ccgttagctt cgccaggaag caaccccgtc
     2701 gcctccggtt gccgcttgcc ggaactaacg gacacggaac gccccgaatc gcgggtttcg
     2761 ttgccgcccg ttgcccctcg gcgcacccca cggccacgca ggtggctgaa tcgcggggag
     2821 cttcgccgtt agttccgctg tggaacaacc gcgtccgctc cggttgccgc cgggcgggag
     2881 tatcggggac ttcctgcctg ttggtgccgt gacgcggaac gaacggggaa tctgcgtcgc
     2941 acatccgcgc caggcgtcgc catccttgcc gtggggcgac acgatcaggc acttcttcac
     3001 cgatacgctc gccacccggc aaccgtccgc gccccgcgac gctaggctga ctccgatgac
     3061 ccgcgacgac cggctccagg ccctcaccga gcggtggcgc gcccgccacg acgcgcgccg
     3121 gccgagcccg gaacgccgcc aatcggctcc gtcggagcgc gaggcactcg cgaagcgcgc
     3181 cttcccgtac cggagcacgc cgccggccga gtacgtcgac gcccatggcg ccgacatgac
     3241 ggccttcacc tacgacgatg agcgctacgc cgatccggac cttgacgcgt ggatcctcga
     3301 agtcgggcgg ctcctccggg aacgaagcgg ccgatgagcg caccgccctg ggcggccgcg
     3361 ctggtgcacc aggtgtgcgc cgatgccggc gtggaaccac ctcgcctgct ctggcggcgc
     3421 cgcaaggacg agcactcgac cggcgtcgcc cggcgctcgg agggcacgat cgccgtgcgg
     3481 gccggccgcg acccaatgga ccagcggctc acgctcctcc acgagctggc ccactggctg
     3541 agccctccgg cacggcgacg ccgtgggagc cggccggccg agcaccacgg gctggccttc
     3601 taccgaatcg ccttcgagct gtaccggcgc cacggcatcc cggccgccga cgccgtgcgc
     3661 ctggaatccg cgcgctaccg cagctcgctg cgccacgcgg ccgcgctcgg catcgatggc
     3721 gccgtcgacg cgcttcgtgc gcaccgcgcc acactgcggg aacggggaag gcgcgagtgg
     3781 cgcgtcctgg tccccgagca tcccatccgg ctcgcgaggg atggccgctg gtcggtgtgc
     3841 gagacgtgcg ggcagcgggt ggtgggaagc aacctggcgc gcgtccgtcg tgcccggcga
     3901 ccggtgcgcc acgtcctgct cggcgccgtc gcctcctagg acgcctgcgc gaccaacccc
     3961 gcgcgctcgc gcgccagcgc ggcggcctcg ccgagacggg cgacggcatc ggtggtgcgc
     4021 gggaagatgt gcgcgatcag gtgggtgacc ccgtactcgg cgtaccgcgc cagcacgtcc
     4081 gccatctcct ccgtcgtgcc gcggatcgcg cgctcgggcg tgtcctcgtc ggcgccctcg
     4141 aacgccacga agatgccggc cgtccgctcg agcgtcgacg gatcacggcc cgccgcctcc
     4201 agcgccccgt cgaggttgct cagccgctcg tggaggtcgg tcgcggtgcg gggatcgccg
     4261 taccaggcgg cgttccactg tgcggcgtgc ttcgcgacga gctccagcat gcgcggctgc
     4321 ttgccggcga tgaggatcgg cgggccgccg gcgcggacgg gcggcggcag gagcacgaga
     4381 tcctgcgctc ggtggaatcg gccctcgaag gtgactgtct caccgcgcag cagcggggcg
     4441 atgatctcca ttccctcggc gaagcggctg acgcggtggt cgaacgggta gccgaacgcg
     4501 tcgaactccg gcttgtgcca cccacacccc agccccagga tcagccgccc gtcactcacc
     4561 tcgtccagct cggccgccat cttcgccacc agcgccgggt tgcggaacgg cagggccaag
     4621 accagcggcc cgatctccac gcgcgaggtg acggcggcca cggcggtgag caccgtccag
     4681 cactcgtgga tgccgacggt ctcgccctcg tcgcggaaga tgagatggtc ggcgccccac
     4741 accgagtcca ggccgctctc ctccgcggcg atcgccatgg cgcgcaggtc ggcgaacccg
     4801 ctggtctcat cggccccgag gggcagcatc agtccgatct tcatgcgccg catcctagcg
     4861 gcgagcgtgc cagcactccg gctagggttg tgtcgtgacc gatccaccgc cgcggctccc
     4921 cctggacggc atccgcgtga tcgacgtcag ccgcgtgctg gcgggaccgt tcgcgaccat
     4981 gctcctggcc gatctcggcg ccgatgtggt caagatcgag ccgcccgagg gcgacgagac
     5041 ccgcacctgg ggaccgccat ggtggggcga tccggcggat cggcggagcg cgtacttcgc
     5101 atcggtcaat cgcaacaagc gcagcgtggt gctggacctg cggacggccg aggggcaggc
     5161 gcggctgcat cggctgctgg cgggggccga tctgctggtc cacaactacc gtccggcaac
     5221 ggctgcgcgc ctgggcctcg agaccgatgc gctccgcgcc cggcatcccc ggctggtggt
     5281 ggcgcacgtc ggcggcttcc cggcccctga cgccgaccgg cccgcgtacg acctcctggc
     5341 gcaggccgtg agcggcctga tgagcgtgac gggcacgccg aacggcccgc cgctcaaggt
     5401 cggcgtggcg ctgctggacc tcatcgccgg attggagtgc gcggtcggcg ccctggccgc
     5461 cctactcggc cgtggccggg tgcgcgaggt ggaggtcggg ctggtcgagg ccggcgtgac
     5521 gtcgctggcc aacgtgctgg ccaaccacct ggccaccggg gaccagccgc cgcggcacgg
     5581 caacgcgcac ccgaacatcg tgccgtacca ggccttcgcg gctcgggacg gcgacctcgt
     5641 catcgccgtc ggcaacgacg cgcagttcgc gcgcctgctg aacgtgctcg ggctcgtcga
     5701 cgccgacgcc cgctttgcca ccaacccgag acgcctcgcg gcgcgcgacg agctgatcac
     5761 ccggctcacc gatgccatcg cggagcgcgg acgcgccgag ctgctggccg cgctcgaggc
     5821 ggccgacgtg ccggccggtc cggtgaacac ggtcggcgag gcggtggccg ccatgggtgc
     5881 cggctgggtg caccaggtcg acgggatccg gctggccccg gacccgatcc gcgtcaacgg
     5941 ggccgcgctc ccggttcgga tgccgccgcc gctcctcggc cagcacaccg cggagctgct
     6001 cgaggacgcc tccgcctccg ccgagcgctg acggccggcc catggccgag cggtatgatg
     6061 ccgagcggat ggacgagtcc cccgcgaccg gcgaccaggc ggcctccttc tcgctcgacg
     6121 agtggatcga gcgcctgcgc acggccctcg gttccgcgga cccgggcatc gagctcgggc
     6181 cggaggagcg ggaggcgatc ctcgagctga cccgcgtggc ggcgcacgcc agtcagcggg
     6241 tggccgctcc gttcaccgcg ttcctcgtgg gcgccgcgct ggacgacctc gccgagccag
     6301 cccgcatcga gcgcctccga gcgctgacga gcatgctgtc gagggccggg accggcgagt
     6361 agcggtcagt ggtcggtggg ctccaggggc agccaccgcg ccgccatggt gaagacgaac
     6421 gcgccgaagg cgaggacgcc gatcacgatg agccactcga tggccgacgg gacgtaccag
     6481 ttggacatgg cgaaggaggc cccgtcctgc ggcgtgccga tcgagacgcc gggcggcagg
     6541 ccgatgggcg agacagccag gccgttgagc accaggttca gccggtgcac gtagatcccg
     6601 acgatcgcga tgccggcggc gatggccacc acccgcggtg acgcccgcca tccccgtcgc
     6661 gcgagcatga cgaacggcac gagcaggccg acgcccactt cgaaccagaa cagcggtgcg
     6721 aactgcccgg tcagcatgcg ggcgatcgga tcgccctcga actccgctcc gggataggcc
     6781 gcggtcagcg cttcggagag gacgaagaag gcctcgacgc tgatgaacac cgccagcagc
     6841 ccgcccaggt acgccaccag gccgtcgtcg aagcgaaggc gcccgacgcg gcgcaccacc
     6901 agcgccagca ggatcaccag cgccagcccg gagaccagcg ccgaggcgac gaacatcggc
     6961 gccatgatcg ccgtgtacca gaagccgcgg ctgatctgga gcccgaagat ccaggccgtg
     7021 atggagtgca gcagcacggc ggccggcagg gcgacccagg ccagcgcccc cttcagccgg
     7081 tcgtcccgtg cgcgtgcctc gaccgacgcc gacgtgccga gcgccagccg gctgccggcg
     7141 gcggcgagat cggcgcgggt gtagagccac acgtacagcg cggacagcgc gaagtacacc
     7201 gtgacgatgg cgatgtccca gatcatcggc gaggtcaggt tcgggtagcg gaacagatgc
     7261 cacaggcgct cggggcgccc gatgtcgggg aagaggaaga tcgcggccag catgaccgcg
     7321 acggtcgcct cgagcacggc cagccgcacg atcgggccga aacgggtcgc cccgaagagg
     7381 cgtcccgacg acgccacgat gagcccgccg gccgagaggc cgacgaagaa catgaagaac
     7441 aggatgtact ggccccacat caccgtgtta cgcaggttcg tgacggccag cccgttgctg
     7501 acctggtaga cccaggcgcc gaaaccgacc agcatgagga gcgccagcag gccgagccac
     7561 agtccgaagc cgccgacgcg ccgttgcgcg cctgccggag cccgcggttc ggcgtgcgcg
     7621 gtcacagctc tcgcctccgc cggggcggca ggtagcgcac cttgggcagc gtccccttct
     7681 cctcgagcag catctcgccc tgccggtcac gcaccaggcg agagacgcgg ctgtccgggt
     7741 cgtcgaggtc gccgaagatg cgcgccccgg ccgggcacga ccacacgcac gacggaacct
     7801 ggccctcctc caggcggtgg acgcacaggg tgcacttctc gacggtaccg acgggccgcg
     7861 cctccaccat gcccgtctcg agtccgttgg gacgggccgg cgccccccag ttgaaggtcc
     7921 gcacgccgta cgggcacgcg atcatgcagt agcgacagcc gatgcagcgc gcgctgtcca
     7981 tcatcaccac gccgtcctcg cgccggtacg tcgcctggac cgggcagacc ttgacgcagg
     8041 gcgcgttgtc gcagtgctgg cacgccaggg gcgtccagct catctcctcc cggccgtgct
     8101 cggtggtcgg caggtcgagc ccctcgccct cggtcaggat gcggttccac cacatgccca
     8161 gcgggacgtt gttctcgctc ttgcagacga cggcgcacga ccagcagccg atgcagcgct
     8221 cggtgtcgac gaccatgccc cagcgcgggc tcgagctcgg cggctcgatc gcgccatagg
     8281 cgcgcgaggc cggcgagagc aagtcgacgg gggagcggcg atcaggcatc ggagctcccc
     8341 gcgatgcggc gcacgtcgca tcggcagtcc ttccagccgg tcgatggcga ccacatgttc
     8401 gccacgaagt ggacctcgtg gatggggttg acctcggatg tcgtcagctc gttgacgccc
     8461 ttgccggcgg cgaagtcgcg tggccaccag ccctccggga gcgtgacgga gccgatgccg
     8521 gccgcctcgc tgacctcggc ccaggcgcgg aacgccccgc gcgtgttgta gacctcgacc
     8581 aggtcgccgg gctcgatccc gcgacgcgag gcatcgtcgg ggtggatgag gagcttcggg
     8641 cgtccgccgt ggatctccgc cagccacggg ttgttgccgt acgtggagtg gatgcgccac
     8701 ttcgagtgcg gggtgaggaa ggtgagcggg tagacggccg ggtcgtgcac gccgtcgtcg
     8761 tgcggatccc ggtacgtcgg gaccgtctcg cccaggtctc ggaagcgctg ctcctccttg
     8821 tacagctcga tgcggcgggt gggcaggaac gccgcggtcg cctcgatcgg cgccggcagc
     8881 gagaccggcg ggaagggctc gaggtcctcg atctgcttca ggaaggggat gtcggggtcc
     8941 gggacgttca ggcgcaccgg gccggcgcgc aggtcggcca gcgtgatgcc cttggtcggg
     9001 ccgtccgcgc ccgcggccag gatcatctcg atcgcctcgt cctcgctgac gtccatggcc
     9061 gccgcctggt cgggagcgat gcgacgcacg agctcgcgcc acatccacag ctccgtgcgc
     9121 gactcgccga ccggatcgat ggcggcctgc tgcagctgga ggaacgggtg gagcggggtg
     9181 gcggtcagat cggtcttctc gtaccaggtc gccgccggga gcacgacatc ggcccagcgc
     9241 accgtctccg tcatctggtg gtcgacgacg acgaccaggt cgagccccgc gagcgtctgg
     9301 tgcagccggt tcacgtcggg cgcctgcacg aacatgttcg cgaaggtgac gacgagtccg
     9361 tggaatccgt tcgccgggta cgggacgtcg gggtgcatgg tgtcggagcg gccgcgcacg
     9421 aagtagatgg acgggacgat gtgcgccttg acgccgtcgg ggttgttcca cgggcccacg
     9481 ttgacgcgga ccttgtactg cccgacgtag acgctgaagc cgccgccgga tcgcccgatg
     9541 ttgccggtca tggacgccag caggcccatg gcgcgccccg tgagatcccc gtggaaccag
     9601 tggttggccc cgccgcccat caggatggca gcgggcttcg tcgctgcgta ggcgtgcgcc
     9661 agccggccga tcatctctgc cgagatgccc gtcacctccg ccgtgcgatc gccggtccag
     9721 cgctccagct cccgccacac cagctcgaac cccggcgcga cctggaccgc ggatccgtcg
     9781 gccagccgca cgcggtaggt gccggatact gcggcccgcg tgccgtccgg catgccgagc
     9841 cgctcggtgc cgacccacgc gggctggccg gctgcctcgt cccagatcgc gaagcgcgcg
     9901 tcgctgccgc cggccacgac gtcggcctcg cggaggcgga caccggtgtc gaggcggacg
     9961 aggagcggcg catcggtgta ggtccgcagg aagtcgaagt cggccagctc gcggacgacg
    10021 atctcccgcg cgaggccgag gccgagcgcg gcatcggtgc cgggacggat ctggagccag
    10081 gtgtcggcct tcgacgccgt gcccgagaag ttcggatcga caacgaccat ctgcgcgccg
    10141 cgctccaggg catcgaagag gaagtggacg tccgggatgc gggtctcgac cgggttggcg
    10201 ccgatgagca gcaggaatcg gctgttcgcc cagtccttcg cctcgtgctc ggcgttctgg
    10261 acgccgaagg tgatcggcat ccccatcggc aggtcaccgt tgaagtcgaa gctcgtgccg
    10321 tgcgtcagcc ccagcagcgc cgatgcgcgg tagttggcac ccttctggat gtagcccgat
    10381 ccgggcacct ggccgaagac gtggatggag tcgtagcccc agcgctcgcc gatgcgcttc
    10441 atgtctccgg cgatccgatc gaggacctcg tcccacgtcg cccgacggaa ctcgccggag
    10501 ccacgctcgc cggtgcggat cagcggcgtg aggatgcggt ccctgccgtg gatcagcgtg
    10561 tggtagctga tgcccttcat gcatccccgc gggttgtacg cggtgtcggg gtagtcggcc
    10621 gcctgctgca ccttcacgac cttgccgtcc cgcacgaacg ccagctgccc gcagctgccg
    10681 gtgcagttcg gggagcaggt ggtccgcacc acgcggtcca ctccttctga cgccgccacc
    10741 gcgggatggt cacgaccgag caggacgaag cccgcgccac cggccgcggc cgcggtcgtc
    10801 gcgccgaccg cggcggctcc cttcaggaag cggcgccgcg agaccgtcat gcccgcaacc
    10861 gttgcggcag gcacgcaccg gagagcatcg tgtggtcggg gctgccgcac gcggcgcagc
    10921 gcgcgtcact ggtctccgta gcgtccggac gaagggtggg aaggaccgat gggcccgccg
    10981 ctgccgcggc acgggccatc ccggtcgcgc cgaacgcgcc gaccagcgcg gcacccgcga
    11041 acagcatgcg gcgacggctc agggcgcgca cgaacagcgg atccacggtg gcacctccgc
    11101 ccgcagtgtc cgggccggag tgcgaccctc gacagggccg gtcggcacgt cgccccggga
    11161 ccatggaccc gaacgacgcg tggtggccgt gcgggttgcg gataccacga ggcccggcgt
    11221 ggcgccgggc ctcgggtcgc gagtcgtcgc tgctctagat gaagccaccc agcgcgtcgc
    11281 gaagctggaa cagctgctga tcctggacgg tgaactcatc gatgagcggt gtcggctgga
    11341 gcagtgccca cgcggcaacg atggcaagga cgacgaggac gaccacggcg gcggcgatga
    11401 gcgtcgcacg cgagatcgcc actggcgccc gaacctgctc gagtcgaggc gcatgctgct
    11461 gaacggtcac ctgcgcgcct cctttcgccc ggacgtcccc cggacaacgg ctccattcgg
    11521 cgccgctggc gagcgctgcg caagagtcga acggcccctt cgacggccga gtccgggcgc
    11581 gcggcgataa cagtcggttt ggcactggtc gctcgcagtg gacgaccacg acgctcaccg
    11641 cctcgatcga cagaggggag agtcccatgc agcacgctgg aacggccggg cggccggcgc
    11701 ggttgatcgc gcggcagcgc cacaccggtc gccgcatcct gctcagcatc accacggcgg
    11761 ccatcttggg tttggcggcc tacctcgtgt acgtcggtgt cgagggttcg agtcggttcg
    11821 tcgcggcgta cgcgagcacc acgtgcacac gcccatccga cctgccctac gggtggacgt
    11881 acgaggcggt caactacgac tccacgctgg acgccggatc cgcgtgcgcc cccgttggcg
    11941 cacgcgcggg cggcgaggtc ttggctgcgg atggaacgcc catcgcgggc tggtacatcc
    12001 cggcggcggc ccggagcggc gacacggcca ccgtcgtcgt ggtgcacggc cacgacgcga
    12061 acaagagcca catgctgcca attgcggcgg cgctccacga ccgattcgac ctgctcctgg
    12121 tcgacctgcg tgcaacgggg tacagcggtg gctcgcagca gaccatgggc gtgctcgagc
    12181 agcaggacgt tcgcgcgatg gtggactggc tggtggagac gaaggcaccc cctcgcattg
    12241 ctgcgcttgg ggtgtcggga ggcgcggccg caacgctcgc ggccatgcga acggacgagc
    12301 gcatcggcgc cttcatcggc gactcgatgc acgcgcggat cggcacgctc ctggcccagc
    12361 aggtggacaa cgccgagctg tgggggatct cgcagccggc ctacccgggc gtctgggccg
    12421 cccaggtcgc aatctggctg cgcaccggcg gcctcgacct cgccagtgtc gatccgatcg
    12481 actcactcgc ctggctggga gaccgcgagc tgctgctgat tcacggcacc gcggacgccg
    12541 aggacgttcc cgctgagagc gtcgacgcga atttcgagga ggctcggcgt ctggggatcg
    12601 atgtcacccg gcgcgactgc acgggtggcg cccatgcggg gctggcgacc gcctgcgcga
    12661 ccgagtacgg aacgtgggtg tccgagttcc tcgacagcgc cttcgccgac tagcggcggt
    12721 cgatgcgctg caggtcctcc ggcgtgttca cgttcgtggt cgacacaccg tccgggtcga
    12781 gcgcgcgcca ccgcgcgggc gggatgagca cgacctcgag atcgtcgatg agatcaagga
    12841 gcctgaacgc gcccctcccg agacggctgg cgatgaccga agtcgcgctc ggccgataca
    12901 ccgcgtggag cggctcgacc ccgtggtccg aaacgagcat caccgcatcg gcagccgggt
    12961 ccgctcgagc ggcatcgatc aggagctcca ggacgtcccg acgaacgagc ggcatgtcga
    13021 ccgcgacgac gatgagcagg tcgctccccg acgcgcggag ggccgcgtcg aggcccgcga
    13081 ggggtcccgc gcctgcgatg gcatcccgca cgagttccgt gcctgatggc gcgtcgacgg
    13141 cctggtccgc gcgggcgacg accaggacgc gatcggcgac ttcggcgagc gtctccgcga
    13201 cccgggacaa cagcgtgcgg ccaccgaaca cgaccccggc cttgtccctg cccatgcgaa
    13261 ggctggcacc gccgaccagg acggcgccgc agatccgttc tcgatggcgc cgtcccggct
    13321 cacgggccac ggtcacggga gttcagccgc gctggtaggg cacgtactgc ggccaccaca
    13381 tcgccgcgtc gacctcggcc tccagcgtct cgggggaacc gccggcgtgg cccgactcga
    13441 tcgcctcccg cccgacggcg atcgccacgg accgagacac ggagcgcagg tcgcgcaccg
    13501 acgggtagag cgccccgcac gcgaggcgct cgggcgtgac ggcggccgcc agggcacggg
    13561 cggcggccag gaccatcgca tcggtgatgt gcgtcgcctc cgcgacgatc gcgccgagcc
    13621 cgagtcccgg gaagacgaag acgttgttcg cctgcccgat ctcgtgcgtc tcgccgccga
    13681 aggtcaccgg cgggaacggc gagccggtcg cgacgagcgc ccgcccaccg gtccagccga
    13741 tcacgtcgcc cggtcgcgcc tccgtcacgg ccgttgggtt ggagagcggc atgaccaccg
    13801 ggcgttcgca tcggtcggcc atcgcgcgga tcacgccctc gtggaaggtc ccggcggtgc
    13861 cggtggtgcc caccaggact gtcggctcca ggcactcgat gatccgcacc aggtccgtcg
    13921 ccttctcccc gtcgatcccg caggcctgcg tcagttccga cggcagcgcg aagtcg
//
LOCUS       DASTYP010000196         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_235546, whole genome shotgun sequence.
ACCESSION   DASTYP010000196 DASTYP010000000
VERSION     DASTYP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235546"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VFV59_08775"
     CDS             complement(<1..1409)
                     /locus_tag="VFV59_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU5325958.1"
                     /translation="MAVLRSTIDPDSDAFAANAAAMRSLVDDLRSRHRALAERGAAGD
                     DRSIARHRERGKLPVRERIDRLIDPGSAFLELSALAATDLYDGDAPGAGIVTGIGQVE
                     GVECVLVANDATVKGGTYYPMTVKKHLRAQEIALENRLPCLYLVDSGGAFLPLQDEVF
                     PDRDHFGRIFYHQAQLSARGIPQVALVMGSCTAGGAYVPAMSDETVIVRGTGTIFLGG
                     PPLVQAATGEVVSAEELGGAEVHTVRSGVADHYALDDEHALAIGRQIVRNLAWRKPDA
                     PWETAEPTEPSVSPDELSGVIPADPRQAYDVREVIARLVDGSEFHEFKERYGETLVCG
                     FARLWGYPVGIVANNGILFSASALKGAHFVELCSQRRIPLVFLQNITGFMVGREYEAG
                     GIARDGAKLVTAVSTTNVPKFTVIIGGSFGAGNYGMAGRAYGPRQLWMWPNARISVMG
                     GQQAARVLSTVRGGFATDA"
     gene            complement(1457..2023)
                     /locus_tag="VFV59_08780"
     CDS             complement(1457..2023)
                     /locus_tag="VFV59_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325959.1"
                     /translation="MSFWNWRRGPGGVVSHGTSAVAVEPDLAPIELYTADARIVAWIA
                     PNGQRVTDLLSTTDVLRLWRPSPGPLDGTDAARSSAGDNGEWEMLPTAQVILAMPPEW
                     RPSRQLRLHKRLRRAALTAGPFSVTGNVHLHPGVDVGLHVARQQPFIPLTDAYLLHNG
                     TPPFEHVVSVVIVNSAHVTQIVPLVTLA"
     gene            2197..2802
                     /locus_tag="VFV59_08785"
     CDS             2197..2802
                     /locus_tag="VFV59_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325960.1"
                     /translation="MTPMIRRILPLAGAMALALAVVVGTALAGGSAADHRTDRADQAR
                     ADAPGLAHAENVNADETEENADEADGAVDPQRFVDRLAAVGIEADRAAFAALVDRLGV
                     GGAVRAFAWASALSDAGTATTAEQVADAFEATPGWGRLARELDPDGTAELRPGIGWIM
                     RGDAGDDASDDAADDATGFGRPDDPGAHGRSRAAAAGADHP"
     gene            2799..3134
                     /locus_tag="VFV59_08790"
     CDS             2799..3134
                     /locus_tag="VFV59_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEU5325961.1"
                     /translation="MIEFEDFLRVDMRVGRVLSAEPFPEARKPSYKLRIDFGPELGER
                     VSSAQLTVTYPDPTTLIGRLVVGVVNFPPRRIAGFKSEVLVLGATGPRGEVDLLRPDP
                     ECEVGLRIG"
BASE COUNT          473 a         1203 c         1215 g          458 t
ORIGIN      
        1 tccgcatcgg tcgcgaagcc gccgcgcacg gtcgagagca cccgcgccgc ctgctgcccg
       61 cccatgacgc tgatgcgcgc gttcggccac atccacagct ggcgcggacc gtacgcgcgc
      121 cccgccatgc cgtagttccc ggcgccgaac gacccgccga tgatgaccgt gaacttcggc
      181 acgttggtgg tgctgaccgc ggtcacgagc ttggcgccgt cgcgcgcgat gccgcccgcc
      241 tcgtactcgc ggccgaccat gaagcccgtg atgttctgca ggaagaccag cgggatgcgg
      301 cgctgcgagc acagctccac gaagtgggcg cccttgagcg ccgaagcgct gaacaggatg
      361 ccgttgttgg ccacgatccc gactgggtag ccccacaggc gcgcgaagcc gcacaccaga
      421 gtctcgccgt agcgctcctt gaactcgtgg aactcgctgc cgtcgaccag ccgggcaatc
      481 acctcgcgga cgtcgtaggc ctggcgcgga tcggccggga tgaccccgga gagctcatcg
      541 ggggagaccg atggctccgt cggctccgcg gtctcccacg gcgcatcggg cttgcgccac
      601 gccaggttgc ggacgatctg gcgaccgatg gccagggcgt gctcgtcgtc cagcgcgtag
      661 tgatcggcca cacctgagcg gaccgtgtgc acctcggccc cgccgagctc ctcggcgctg
      721 accacctcgc cggtcgccgc ctgcaccagc ggcggtccgc cgagaaagat ggtgccggtg
      781 ccgcgcacga tgacggtctc atcgctcatg gccggcacgt aggcgccgcc ggcggtgcaa
      841 gagcccatga cgagcgcgac ctgcgggatg ccgcgcgcag agagctgggc ctggtggtag
      901 aagatgcgcc cgaagtggtc gcggtcgggg aagacctcgt cctggagcgg caggaaggcg
      961 ccaccggagt ccaccaggta gaggcacggc agccggttct ccagcgcgat ctcctgcgcc
     1021 cgcaggtgct tcttcaccgt catcgggtag tacgtgccgc ccttgaccgt cgcgtcgttg
     1081 gcgacgagca cgcactccac cccctccacc tggccgatac ccgtcacgat gcccgcgcct
     1141 ggcgcatcgc cgtcgtacag gtccgtggcg gccagggcgg agagctcgag gaacgcggag
     1201 ccgggatcga tcagccggtc gatgcgctcg cggacgggca gcttgccgcg ctcacggtgc
     1261 cgcgcgatgg atcgatcgtc accggccgct ccgcgctcgg cgagggcccg atgccggctt
     1321 cgcaggtcgt ccacgaggga gcgcatggcg gccgcgttcg ccgcgaacgc atcggagtcg
     1381 ggatcgatcg tggagcggag gacggccatc ggcgccgatt atggcgccgc tcggcggcga
     1441 gccggagccg ccgcagtcag gcgagggtga cgagcgggac gatctgggtg acgtgcgcgc
     1501 tgttgacgat caccacgctg accacgtgct cgaacggcgg cgtgccgttg tggagcaggt
     1561 acgcatcggt cagggggatg aagggctgct gacgcgcgac gtgcagtccg acgtcgacgc
     1621 cgggatgcag gtgcacgttg ccggtgaccg agaacggacc tgcggtgagg gccgcgcgac
     1681 ggagtcgctt gtgcagccgc agctggcggc tgggacgcca ctccggtggc atggccagga
     1741 tcacctgcgc cgtcggcagc atctcccact cgccgttgtc gccggccgac gagcgggcgg
     1801 catcggtccc gtccagcggt ccagggctgg ggcgccagag gcgcagcaca tcggtcgtgc
     1861 tgagcaggtc ggtcacgcgc tgcccgttcg gcgcgatcca cgcgacgatg cgggcatcgg
     1921 ccgtgtagag ctcgatgggc gcgaggtcgg gctcgaccgc gaccgccgag gtcccatgac
     1981 tgaccacgcc gccgggtccg cgacgccagt tccagaacga cacgccatgc ctccgatgag
     2041 ggtgggggcg cgcggggcac gtggcgcgcc ccccgatgcg aaccgtacgg accgacggga
     2101 cgcgcaccaa cggcccgata gtcctagtcg agggccggtg cgtggcggca cccaggcggc
     2161 tggaccggca ggccgaccga gggctatgct cgaccgatga cgccgatgat tcgccgcatc
     2221 ctccccctcg ccggcgccat ggcgttggcc ctcgccgtcg tcgtcgggac ggccctcgcc
     2281 ggcggcagcg ccgccgacca tcggaccgat cgtgccgacc aggcacgggc cgatgccccg
     2341 ggcctcgccc acgccgagaa cgtcaacgcg gacgagaccg aggagaacgc cgacgaggcc
     2401 gatggcgccg tcgacccgca gcggttcgtg gaccggctcg cggccgtcgg catcgaggcc
     2461 gatcgggcag ccttcgccgc gctggtcgat cggctgggtg tgggtggtgc ggtacgcgcg
     2521 ttcgcctggg cctcggccct cagcgacgcc gggaccgcca cgaccgccga gcaggtcgcc
     2581 gacgcgttcg aggcaacccc cggctggggt cgcctggccc gtgagctcga cccggacggc
     2641 accgcggagc tgcggccggg gatcggctgg atcatgcgag gcgacgccgg ggacgacgcc
     2701 tccgatgacg ccgccgacga cgcgactggc ttcggccgcc cggatgatcc gggcgcgcac
     2761 ggccgttccc gcgccgcggc ggccggcgcc gaccacccct gatcgagttc gaggacttcc
     2821 tgcgcgtcga catgcgcgtc ggccgcgtcc tgtccgccga gccgttcccg gaggcgcgca
     2881 agccgtcgta caagctgcgc atcgacttcg gccccgagct cggcgagcgc gtcagctcgg
     2941 cgcagctgac ggtcacctac ccggatccca ccacgctcat cggccgcctg gtcgtgggcg
     3001 tggtgaactt cccgccgcgg cgcatcgcgg ggttcaagag cgaggtgctc gtcctcggcg
     3061 ccacgggtcc ccggggagag gtggacctcc tccggcctga tccggaatgc gaggtgggcc
     3121 tgcgcatcgg ctgatccgac ggacccgcac ggtccgtaga ggtgggcaca accccgaatt
     3181 gcccactgga ggattcctct ccgatgcgaa cgctctcccg cctcgccggc atgctggccg
     3241 gcacggcgct ggttgccgcc ctcgcggttc ccgtggccgc agccgatgac gccatggtgc
     3301 gcgtcgtcca cgcctcgccc gacgccccga acgtcgacgt ctgggtcga
//
LOCUS       DASTYP010000197         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_818710, whole genome shotgun sequence.
ACCESSION   DASTYP010000197 DASTYP010000000
VERSION     DASTYP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818710"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VFV59_08795"
     CDS             complement(<1..981)
                     /locus_tag="VFV59_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5325962.1"
                     /translation="MPDSATTTPPPRPRLAPQEILDRLNPPQREAVSHVSGPLLILAG
                     AGSGKTRVLAHRVAYLIASGVKPWQIVAVTFTNKAAGEMRERIAGLIGEVEAREATIG
                     TFHAICARILRRDGAAIGLTRSFTIYDRADQVALVKSVLRRLDLDEKRFSPAGMLAWI
                     GQRKDELADVATAKRQAANYYDETAAGVYEAYQRQLGEDDAVDFDDLLMRTVFLFEQH
                     PDVLARYQGRWQQILVDEYQDTNRAQYLICRHLAAKHRNLAVVGDDDQSIYSWRGADL
                     RNILDFEADYPDAKVVKLEQNYRSTQTILDAAHAVVSRNVGRKDKKLWTDR"
     gene            1227..1745
                     /locus_tag="VFV59_08800"
     CDS             1227..1745
                     /locus_tag="VFV59_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325963.1"
                     /translation="MTDAARIRPIRPDEHDAVADLTVRAYRARFEDLGDYEGVLRRVG
                     HRASRGTVLVAVMAGQLVGTVTYVRGPGPYAEGDDPDAAWIRMLAVAPEQQGRGLGRA
                     LTIACIERARADGRRRIALHTGDTQLAAQRIYAGLGFVRRPALDELVDDEFWLRGWVL
                     ELEEEEEAQPRS"
     gene            complement(1730..2098)
                     /locus_tag="VFV59_08805"
     CDS             complement(1730..2098)
                     /locus_tag="VFV59_08805"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5325964.1"
                     /translation="MRSVLMNYVHPDDATAYYALTPEEQQADVERHIAWFRKHGEHIV
                     GGEELGEPQRVKTLRRGRQGEGVVVTDGPFVETKELLGGFVILETDTLEQALAIASEW
                     PSLTSQPNATVQLHPVYERG"
BASE COUNT          316 a          832 c          814 g          372 t
ORIGIN      
        1 ccgatcggtc cacagcttct tgtccttgcg tccgacgttg cggctcacga ccgcgtgggc
       61 ggcgtccagg atcgtctggg tcgagcggta gttctgctcg agcttcacga ccttcgcatc
      121 ggggtagtcg gcctcgaagt cgaggatgtt ccgcagatcg gcgccgcgcc acgagtagat
      181 gctctggtcg tcgtcgccca ccacggccag attgcggtgc ttcgcggcca ggtggcggca
      241 gatcaggtac tgcgcgcgat tggtgtcctg gtactcgtcg acgaggatct gctgccagcg
      301 tccctgatac ctcgcgagca cgtcggggtg ctgctcgaag aggaacaccg tccgcatgag
      361 gaggtcgtcg aagtcgacag catcgtcctc gcccagctga cgctggtacg cctcgtacac
      421 gccggccgcc gtctcgtcgt agtagttcgc ggcctgccgc ttcgccgtcg cgacgtcggc
      481 cagctcgtcc ttgcgttgcc cgatccaggc cagcatgccg gccgggctga agcgcttctc
      541 gtccaggtcg aggcggcgca gcacggactt gacgagcgcc acctggtcag cccggtcgta
      601 gatggtgaag ctgcgggtca gaccgatggc ggcgccgtcg cgccgcagga tgcgcgcgca
      661 gatggcgtgg aaggtgccga tggtcgcctc gcgcgcctcg acctcgccga tgagccccgc
      721 aatgcgctcc cgcatctcgc cggctgcctt gttcgtgaag gtgactgcca cgatctgcca
      781 tggcttgacg ccgctggcga tcagatacgc gacccgatgc gcgagcacgc gggtcttgcc
      841 gctccccgca ccggcgagga tgaggagcgg accggagacg tggctgaccg cctcccgctg
      901 cggcgggttc agacggtcga ggatctcctg gggcgcgagc cgtgggcgtg gcggcggcgt
      961 agtggtggcg gagtcgggca tctccccgat tctaggggcg cccggagtgg accgcagccg
     1021 ccggcccggt tctcagcgct ctcggtcgat gtggacgcgg atccgatccg gctcggcctg
     1081 ccggccgtgc tccagcacga cgccgacggc caggaggatc agggcaatgg cgagttccat
     1141 gcccctccca atgcagcgac cgtgccacgc ccgcccgaac gcgcgtctgg ccggtgcatc
     1201 ggcgcgacat cccgcatagt cgtgccatga ccgatgccgc ccggatccgc cccatccgcc
     1261 cggacgagca cgacgccgtc gccgacctga cggtgcgcgc ctaccgcgcg aggttcgagg
     1321 acctcggcga ctacgagggt gtgctgcgtc gggtcggcca tcgcgcatcg cgcggcacgg
     1381 tgctcgtggc ggtgatggcc ggccaactcg tcgggaccgt cacctacgtc cgaggcccgg
     1441 ggccgtacgc cgagggcgac gacccggacg cggcctggat ccgcatgctg gccgtcgccc
     1501 cggagcagca gggccggggg ctcgggcggg cgctgacgat cgcgtgcatc gagcgagcgc
     1561 gagccgacgg ccgccgtcgc atcgccctcc acacgggcga cacccagctc gccgcgcagc
     1621 gcatctacgc cggcctgggc ttcgtccgcc gtcccgccct cgacgagctg gtggacgacg
     1681 agttctggct gcgcggctgg gtcctcgagc tggaggagga ggaggaggct cagcctcgct
     1741 cgtagacggg gtggagctgc accgtcgcgt tcggctggct ggtcaacgac ggccactcgg
     1801 acgcgatcgc cagcgcctgc tccagcgtat cggtctcgag gatcacgaag ccgccaagca
     1861 gctccttggt ctcgacgaac ggcccgtcgg tgaccacgac gccctccccc tgccgccccc
     1921 gccgcagcgt cttcacccgc tgcggctcgc ccagctcctc gccgccgacg atgtgctcgc
     1981 catgcttgcg gaaccacgcg atgtggcgtt cgacatcggc ctgctgctcc tcgggcgtca
     2041 gggcgtagta cgccgtggcg tcgtcggggt ggacgtagtt catcaggacg ctgcgcatcg
     2101 gttcagtccc tcgtctgtgc ggcgtccagc ggacgccatt cgaaccctac gacgcggcgc
     2161 ggttgcggct ctcgacaaaa cgtcgacgcg gtaccgtgcc gtcatgaacg actgcgtctt
     2221 ctgcggtcag ccgatccagc cggaccagcc gacggccggc cgaccgccca tggccgccca
     2281 cgccacctgc gccgatgcgg ccctcgccga tgacggccac tgggacgcgg tcgc
//
LOCUS       DASTYP010000198         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_490919, whole genome shotgun sequence.
ACCESSION   DASTYP010000198 DASTYP010000000
VERSION     DASTYP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490919"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1412)
                     /locus_tag="VFV59_08810"
     CDS             complement(174..1412)
                     /locus_tag="VFV59_08810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU5325965.1"
                     /translation="MRDAVIVSAVRTAVGKAPRGTLRETPPSELGMVAVREALVRAPG
                     LDPAEVEDLILGCAMPEGAQGLNIARQVALGAGIPMSASAMTVNRFCASGLQALALAT
                     QAVQTGVHDVVVAGGMESMSYVPMIGHHFAPDPTMADTRPQVYLSMGLTAENVAEQYG
                     VSRDDQDAWGLRSHQRASAAQREGRFSDEVVPVAVRLTELDADGRPSVRETAFDEDEG
                     IRHDADAGQMAGLRPVFAAGGSVTAGNSSQMSDGAAAMVVMTGERAAALGLTPLGRLV
                     SFATAGVDPAIMGVGPVEAVPKALAKAGVSLDDIAVTEFNEAFAAQVVAVVRELGLNE
                     ATTNVNGGAIALGHPLGATGAKLTVQLLSELARRGARYGLVTMCVGGGQGAAGVIEWL
                     GGRQPARTAAAPTAHTERAA"
     gene            complement(1437..>2579)
                     /locus_tag="VFV59_08815"
     CDS             complement(1437..>2579)
                     /locus_tag="VFV59_08815"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5325966.1"
                     /translation="LGPFQLLDALGQDAERLLAELERPSPAYENGSSHSFATGALEPR
                     PARPGSVDLAAAQRAELPSNAAATTLDLGDGILGLELHGKLNIIGLDTIAMLERAVAL
                     ATERYDGLVIGTQAADFSAGANLALLLLEAEEGEWQELDRVVRRFQGAAMALRHSPIP
                     VVVAPRGRTLGGGAELVLAADRAQPLAETYIGLVELGVGLIPAGGGSTAMARRIAERL
                     PDGARGDLFPLYGHVFTAVASATVATGAFEARRYLFLRDADPITADPDRQWADAARTA
                     RTLAQNGYRPPGETRIPVLGRRGIAAAESLSYNQLVARQISEYDRRLVLELAHVMSGG
                     DVAEGTAVSEQHLLDLERQAFLRLLGERMTRDRIRHILKTGKPLRN"
BASE COUNT          331 a          937 c          960 g          351 t
ORIGIN      
        1 gcggccgaac ggtcgcaatt cacgcgaggt gtgcttcggc cgcgtggcgg gccggccgaa
       61 cggtcgcaat tcacgccgga tgtgaagaga ggcgcttcgc gccaccactc cgggcgcgga
      121 acgctgcgac ggagcgacgg atgcgcgcgc ggtgtgagtt cggacctttc gcgtcatgcg
      181 gctcgctccg tgtgcgccgt cggcgccgcg gccgtgcgcg ccggttgccg cccgccgagc
      241 cactcgatca ctccggccgc gccctggccc ccgcccacgc acatggtcac cagcccgtat
      301 cgcgccccgc ggcgggccag ctcggagagg agctgcacgg tcagcttggc gccggtggcg
      361 ccgaggggat ggccgagcgc gatggccccg ccgttcacgt tggtcgtcgc ctcgttcagc
      421 ccgagctcgc ggaccacggc cacgacctgg gcggcgaagg cctcgttgaa ctcagtgacc
      481 gcgatgtcgt cgaggctcac ccccgccttg gcgagggcct tgggaaccgc ctcgaccgga
      541 cccacgccca tgatggcggg gtcgacgccg gcggtcgcga agctcaccaa ccggcccagc
      601 ggcgtcagac ccagggccgc ggcgcgctcg ccggtcatga ccaccatggc ggccgcccca
      661 tcgctcatct gcgaggagtt gccggccgtg acgctcccgc cggcggcgaa gacggggcgc
      721 aagccggcca tctggccggc atcggcgtcg tggcggatgc cctcgtcctc gtcgaacgcc
      781 gtttcccgga ccgatggacg gccgtcggcg tcgagctcgg tgagccgcac ggcgaccggc
      841 acgacctcgt cgctgaaccg gccctcgcgc tgagcggccg aggcccgctg gtgggagcgc
      901 aggccccagg cgtcctggtc atcgcggctg acgccgtact gctcggcgac gttctcggcg
      961 gtgaggccca tcgagaggta gacctgcggc cgcgtgtcgg ccatcgtcgg gtcgggcgcg
     1021 aagtggtggc cgatcatcgg gacgtagctc atcgactcca tcccgccggc tacgaccacg
     1081 tcgtggacgc cggtctgcac cgcctgcgtg gcgagggcca gcgcctggag gccggaggcg
     1141 cagaaccggt tgacggtcat cgccgatgca ctcattggaa tcccggcccc cagcgccacc
     1201 tgtcgggcga tgttcaggcc ctgcgccccc tccggcatgg cgcagccgag gatcaggtcc
     1261 tcgacctcag ccgggtcgag gccgggagcc cgtacgagcg cctcccgcac ggcgaccatg
     1321 ccgagctccg acggtggcgt ctcccgcagc gtgccgcgcg gcgccttgcc gaccgcggtc
     1381 cggacggcgg agacgatgac ggcgtcacgc agctggttca tcggtccatc tcctcgtcag
     1441 ttccgcagcg gcttgccggt cttgaggatg tggcggatgc gatcgcgtgt catccgctcg
     1501 cccaggaggc gcaggaacgc ctggcgctcg aggtcgagca ggtgctgctc gctcaccgcc
     1561 gttccctcgg ccacgtcccc accgctcatc acgtgcgcca gctccaggac caggcgccgg
     1621 tcgtactcgc tgatctggcg ggcgaccagc tggttgtagg acagcgactc cgcggcggcg
     1681 atgccgcgac gaccgaggac cgggatgcgg gtctcgcccg ggggccggta gccgttctgc
     1741 gccagggtgc gggcggtgcg agccgcatcg gcccactggc ggtcggggtc cgcggtgatg
     1801 gggtcggcgt ccctcaggaa gaggtagcgg cgggcctcga aggcgccggt ggccacggtg
     1861 gccgaggcga ccgcggtgaa gacgtgcccg tacagcggga agaggtcgcc ccgcgccccg
     1921 tccggcagcc gctcggcgat ccgccgcgcc atggcggtgg agccgccgcc ggccgggatc
     1981 aggccgacgc ccagctcgac caggccgatg taggtctcgg ccagcggttg ggcccggtcc
     2041 gcagccagga ccagctccgc gccaccgccc agcgtgcggc cgcgcggcgc gaccaccacg
     2101 gggatcggcg agtgccggag cgccatggcc gcaccctgga agcggcgaac cacccggtcg
     2161 agctcctgcc actcgccctc ctcggcctcc aggagcagga gcgccaggtt ggcgccggcg
     2221 ctgaagtcgg ccgcctgcgt gccgatgacc aggccgtcgt agcgctcggt tgcgagggcg
     2281 acggcgcgtt ccagcatggc gatggtgtcg aggccgatga tgttcagctt tccgtgcagt
     2341 tccagcccca ggatcccgtc gccgaggtcg agggtcgtgg ccgcggcgtt ggagggcagc
     2401 tccgcccgtt gggcggccgc gaggtcgacc gatccggggc gcgccggccg cggctccagg
     2461 gcgccggtcg cgaacgaatg cgaggagccg ttctcgtagg ccggcgaggg tcgctcgagc
     2521 tccgcgagga gccgctccgc gtcctgcccg agtgcgtcca gcagctggaa cggcccgag
//
LOCUS       DASTYP010000199         7237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_926732, whole genome shotgun sequence.
ACCESSION   DASTYP010000199 DASTYP010000000
VERSION     DASTYP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926732"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..976)
                     /locus_tag="VFV59_08820"
     CDS             complement(47..976)
                     /locus_tag="VFV59_08820"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5325967.1"
                     /translation="MTLNFRGLIAATVLPMDADARVDEAALRAYIRWVADQGVVGVAI
                     NADTGESAHLAHAEKVRVLEVVREELPSNVMMVAGLGGPYTDVAVQQAKDYRAAGADA
                     LLVFPIPAYLSSPLDPEIPVRYHQAIAEVGVPLILFQLQPALGGVNYDRATLERLLAI
                     DGVAALKEASFDARRYMDTVAMVRASGRDVAMLTGNDNFIYESFLLGAEGALIGFGAV
                     MTREQVELIRLAHEHRYEEAAPLAARVQRLADAVFAPPVANYRARLKEALVMLGVLEN
                     ATVRTPLLPISETERRALREVLAEVGLLEAVAA"
     gene            1126..2190
                     /locus_tag="VFV59_08825"
     CDS             1126..2190
                     /locus_tag="VFV59_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5325968.1"
                     /translation="MASIVDVARLAGVSPATASRVLSGSTHAVSQVTRSRVEAAAAEL
                     DFVPNALARGLLKSRAPIVGVIVHDITDPYFSEIVRGIEDEAEASGYLVITSSSDRIP
                     AREESYVRLLRGMRATAVLFAGSGIRNAEFEERIGRHLVAMERYGAAVVHLSPSARGP
                     ALVGVDNVAAMAEVVRALVALGHRRIGYVGGPEWLYVAADRERGYRSALAEAGIGIDP
                     GLVVTASFDQDGGADGAERLLRADPRLTAIACANDLQALGALDRFAQLGVSVPDDVSV
                     TGFDDIPGASRTSPSLSTVHLPLRELGRSGFLQAEKILAGRRPHRRLLAHEIVLRRST
                     SKPRRAARVAVDVPAALVAS"
     gene            2187..3008
                     /locus_tag="VFV59_08830"
     CDS             2187..3008
                     /locus_tag="VFV59_08830"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325969.1"
                     /translation="MTAAATAGATAGRGRVAGHQIEIRNLSKRYRTTRSTQEALKDVS
                     LDIAAGEFVSLLGPSGCGKTTLLKIIGGLVTRSSGEVRIDGGSVDDALRARKFGVVFQ
                     DATLLPWRTVLGNARLLLDITGERDQAAPVDDLLRTVGLGGFEDFYPSQLSGGMRQRV
                     ALARALALSPEILLMDEPFAALDAITRDRMGEELLRIWDGSRTVVFVTHSIPEAVLLS
                     DRVVVLSARPGRIRADVTIDLPRPRSGQVRASPAFTRYEAELRQYIEETSNGTGD"
     gene            2992..3843
                     /locus_tag="VFV59_08835"
     CDS             2992..3843
                     /locus_tag="VFV59_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5325970.1"
                     /translation="MAQATDEVRVGAADLVDRDDAELLPEGRRPWQRMVLPVLLLVAI
                     VVVWELASRFTDVPAYILPAPTAIVERLVRSFPLLWEHSVVTLTEIGIGFALGVALGL
                     GLAIPIAYSAVVRNTLYPLIVASQAVPKIAIAPLLVLWLGFDLWPKIVVTALMVFFPV
                     TVTAAEGFSSVDRNLLDLLRSVHANQAQIFFKVRFPHALPQIFAGLKIGITLAVVGAV
                     VGEWVGADAGLGYLLVYANTLLDSTLLFASLILLIVIGVVLFLIVGVIERLLLPWHRD
                     ESTYLGT"
     gene            3950..5002
                     /locus_tag="VFV59_08840"
     CDS             3950..5002
                     /locus_tag="VFV59_08840"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5325971.1"
                     /translation="MKGMPRYLALLLGLMFVLAACGQGDESSAAATAEPSASTAPASD
                     APSDEATPEPTEVAMTSVDFRLNWVLAGNHAPFFLAKQEGFWEECGLDVSMAAGQGSG
                     DTAQQVANGSQEFGLTDAVSIVAGRTRGLEITALGVLYQTNPSAIVSKKSAGIEALED
                     VQGKTWGAVPGGSPYLLLNALFQENDITDVREVSVPAPGIAQLLADQVDFITFFGNEV
                     ANIDPNPDENLNVVPFSDYGQDIYGLAIASHPDYIAENPDQVACFVDGVRRGFEAARE
                     DPEAALDALEAANPETAERREVHAKLLEGAFEYAGDDLLVQSEEKWAETQDVLSSAGI
                     IESTVEPSDFFTNDYQ"
     gene            5074..5826
                     /gene="fabG"
                     /locus_tag="VFV59_08845"
     CDS             5074..5826
                     /gene="fabG"
                     /locus_tag="VFV59_08845"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEU5325972.1"
                     /translation="MTLLQDRVVLVTGSSRGIGAEVAVRAAAVGARVAIHCHSHPEGA
                     ERTLQRVREAGGEGAVFRADLADGGEAEELVAGVIGHFGRIDGLVNNAGRTQVGPFLD
                     LKPHEWDAVIATDLSAAFHTSRAALPSMVERGSGAIVNVASRLGQMGITQTAAYSAAK
                     AGLIGLTRSLAREFAPYGVRINAVAPGFTVTEMTADLAETETGRQRMRDIPLGRFGEA
                     GEVADAVVFLLSDAAALFVGQTLNPNGGSYMP"
     gene            5823..>7237
                     /locus_tag="VFV59_08850"
     CDS             5823..>7237
                     /locus_tag="VFV59_08850"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU5325973.1"
                     /translation="MTKIISVREAVGKVEDGKTVLIGGSGAGHAVPQRFIDELAAVFQ
                     EQGRPRDLTTVRIVGIGDFADRGFSQLGLPGLMRRTIGSNIGNEPGLGALVEAGQLEA
                     YSFPQGVLSQLTREIAAGRPGLVTQVGLGTYVDPRHTGGKQNAVTTEDLVEVVTLRGR
                     EWLFFHAFPIDVAVIRGTTADEDGNITMEGEAVQGEMLAMAMAARNSGGIVIAQVKRL
                     GRRGSLPLRDVKVPGALVDYVYVDDHQWQTYVTQDSPYYAGALRKPVTPEPPLPLDVR
                     KIIARRSLLEFPPGAICNLGFGISQLIGRVAWEEGITDQLTLTVEQGIFGGVPVAGNE
                     GGAGFNYQAMIDQPYMFDFYDGGGLDIASLSFAEVDAEGNVNVHAFEGRVRGPGGFPN
                     ISARTPRINFVGTLTAQGLELDIDDGVRIVSEGRLSKFVPKVREISFNGHLARERGQQ
                     VRYITDRAVFALTDDGLELIEV"
BASE COUNT         1046 a         2532 c         2571 g         1088 t
ORIGIN      
        1 cgcggaacgc cggcgtccag gatcgtggcg aggcgcatcg gcgccgtcag gcggccacgg
       61 cctcgagcag tccgacctcg gccagcacct cacggagggc acgacgctcg gtctcgctga
      121 tcggcagcag tggcgttcgg accgtcgcgt tctcgaggac gccgagcatg accagcgcct
      181 ccttgaggcg ggcgcggtag ttcgcgacgg gcggcgcgaa gacggcatcg gccaggcgct
      241 ggacccgtgc ggcgaggggc gcggcctcct cgtagcggtg ctcgtgggcg aggcggatca
      301 gctcgacctg ctcgcgcgtc atcaccgcgc cgaagccgat gagcgccccc tcggcgccga
      361 gcaggaagga ctcgtagatg aagttgtcgt tgccggtcag catcgcgacg tcgcggccgg
      421 aggcgcggac catcgccacg gtgtccatgt agcgacgcgc atcgaaggac gcctccttca
      481 gcgccgcgac cccgtcgatg gccagcagcc gctcgagcgt tgcgcggtcg tagttcacgc
      541 cgcccagcgc cggctgcagc tggaacagga tcagcggcac gccgacttcg gcgatggcct
      601 ggtggtagcg gaccgggatc tccgggtcca gaggcgagga gaggtaggcg gggatcggga
      661 agacgagcag cgcatccgcg cccgcggcgc ggtagtcctt cgcctgctgg accgcgacgt
      721 cggtgtaggg cccgccgagc cctgcgacca tcatcacgtt cgacggcagc tcctcgcgga
      781 cgacctccag cacgcggacc ttctcggcat gcgccaggtg cgcggactcg cccgtgtcgg
      841 cgttgatcgc cacgccgacg acgccctggt ccgcgaccca tcgaatgtac gcgcgcagcg
      901 cggcctcgtc gacgcgcgca tcggcgtcca tcggcaggac ggtggcagcg atcaggccgc
      961 ggaagttgag agtcatcgga cgcactccag cagtgatgtt gtggcacagg atactgcccc
     1021 ggccgcgcgg tggaaatcct tttctctcgg catgggcagg aatccttgac gctgccgcgg
     1081 cgcacgacct agcctactgc aaaccttttc ccacacgggg tgtccatggc ctcgatcgtc
     1141 gacgttgctc ggctggccgg tgtctcaccc gcgaccgcgt cgcgggtgct gtccgggtcc
     1201 acccatgccg tgagccaggt gacgcggagc cgggtcgagg ccgcggcggc ggagctcgac
     1261 ttcgtcccca acgcgctcgc ccgcggcctg ctcaagagcc gggccccgat cgtcggcgtg
     1321 atcgtccacg acatcaccga cccgtacttc tcggagatcg tgcggggcat cgaggatgag
     1381 gcggaggcga gcggctacct ggtcatcacg tcctcctcgg accggatccc ggcgcgcgag
     1441 gagtcgtacg tgcggctgct gcgcggcatg cgcgcgaccg ccgtcctctt cgccggcagc
     1501 gggatccgca acgccgaatt cgaggagcgg atcggccgcc acctcgtcgc catggaacgc
     1561 tacggagccg ccgtggtgca cctgtcgccc agcgcacgcg gtccggcgct cgtcggcgtc
     1621 gacaacgtcg ccgccatggc cgaggtcgtg cgggcgctcg tggcgctcgg ccatcggcgc
     1681 atcggctacg tcggagggcc ggagtggctg tacgtcgccg ccgatcgcga gcgtggctac
     1741 cggagcgcac tggccgaggc gggcatcggc atcgaccccg gcctcgtcgt cacggcctcg
     1801 ttcgaccagg acggtggcgc ggatggcgcc gaacgcctgt tgcgagccga cccgcgtctg
     1861 acggccatcg cctgcgccaa cgacctccag gcgctcggcg ccctcgaccg cttcgcgcag
     1921 ctcggcgtct cggtgcccga tgacgtgtcg gtgacgggct tcgatgacat cccgggcgcg
     1981 tcacgcacca gccccagcct ttcgacggtg cacctcccgc tgcgcgagct cggacgttcc
     2041 ggcttcctgc aggcggagaa gatcctggcc ggtcggcggc ctcaccgccg gctgctggcg
     2101 cacgagatcg tcctgcgccg ctcgacctcg aagccgcgtc gtgccgcacg ggtcgccgtc
     2161 gacgtcccgg cggcgctggt ggcgtcatga cggccgccgc caccgccgga gcgaccgcgg
     2221 ggcgcggccg cgtggcaggt caccagatcg agatccgcaa cctctcgaag cgctaccgga
     2281 cgacgcgctc cacgcaggag gcgctgaagg acgtctcgct cgatatcgcg gccggagagt
     2341 tcgtctcgct gctcggaccg tccggctgcg gcaagacgac gctgctgaag atcatcggcg
     2401 ggctcgtcac gcggagcagc ggggaggtcc gcatcgacgg cggaagcgtg gacgacgcgc
     2461 tccgggcccg gaagttcggg gtcgtcttcc aggacgcgac cctgctgccg tggcgcacgg
     2521 tcctgggcaa cgcgcggctg ctgctggaca tcaccggtga gcgggaccag gcggcgccgg
     2581 tcgacgacct gctgcgcacg gtcgggctcg gcggcttcga ggacttctac ccgtcgcagc
     2641 tgtccggcgg catgcgccag cgcgtcgcgc tcgcgcgggc gctcgcgctc agcccggaga
     2701 tcctgttgat ggacgagccg ttcgccgcgc tcgacgccat cacccgcgac cggatgggtg
     2761 aggagctgct ccgcatctgg gacggcagcc gcaccgtggt cttcgtcacc cactccatcc
     2821 ccgaggcggt gctgctgagc gaccgggtgg tcgtgctgtc cgcacgtccg gggcgcatcc
     2881 gcgcggacgt cacgatcgac ctgcctcgtc cgcggagcgg gcaggtgcgg gcgagcccgg
     2941 cgttcacccg ctacgaggcc gagcttcgcc agtacatcga ggagacgtcg aatggcacag
     3001 gcgactgacg aggtccgcgt cggtgccgcc gacctggtcg accgcgacga cgcggagctg
     3061 cttccggagg ggcggcggcc gtggcagcgc atggtcctgc cggtgctgct gctcgtcgcg
     3121 atcgtggtcg tctgggagct cgcgtcccgc ttcaccgacg tgccggcgta catcctcccg
     3181 gcgcccacgg cgatcgtcga gcggctggtc cggtcgttcc cgctgctgtg ggagcacagc
     3241 gtggtgacgc tgaccgagat cggcatcggc tttgcgctgg gcgtggcgct ggggctgggc
     3301 ctggccatcc cgatcgccta ctccgcggtg gtgcgtaaca cgctctaccc cctgatcgtg
     3361 gcgtcgcagg cggtgccgaa gatcgcgatc gcgccgctcc tcgtcctgtg gcttgggttc
     3421 gacctgtggc ccaagatcgt ggtcaccgcc ctgatggtct tcttcccggt caccgtcacc
     3481 gcggccgagg gcttctcctc ggtcgatcgc aacctgctgg acctgctgcg ttcggtccac
     3541 gccaaccagg cgcagatctt cttcaaggtc cggtttccac acgcgctgcc ccagatcttc
     3601 gcgggcctga agatcggcat caccctcgcc gtggtgggtg ccgtcgtggg cgaatgggtc
     3661 ggcgccgatg cggggctggg ctacctgctg gtgtacgcga acactctgct cgactcgacc
     3721 ctgctgttcg cgtcgctgat cctgttgatc gtgatcggcg tggtgctgtt cctcatcgtg
     3781 ggtgtcatcg aacggctgct gctgccgtgg caccgcgacg agtcgaccta cctcggaacc
     3841 tgaggccaac ggagacacgt tccatcgacg gcggagcatt ggaggtgagg aaaaggattt
     3901 ctagcccggt cgaccgcgaa ggattgatga acgaaggagg acacgggaga tgaaggggat
     3961 gccccgctac ctggcactgc tgctgggcct gatgttcgtg ctcgccgcgt gcgggcaggg
     4021 ggacgagtcg tcggccgcgg cgaccgcgga gccgtcggcg agcaccgccc cggccagcga
     4081 tgcgccgtcc gacgaggcca cgcccgagcc gaccgaggtc gccatgacca gcgtcgactt
     4141 ccggctcaac tgggtgctcg ccggcaatca cgcacccttc ttcctggcca agcaggaggg
     4201 cttctgggag gagtgcgggc tcgacgtgtc gatggccgcc gggcagggct cgggcgacac
     4261 ggcccagcag gtcgccaacg gctcgcagga gttcggcctg accgacgccg tctcgatcgt
     4321 ggcgggccgg acccggggcc tggagatcac cgcgctcggc gtcctgtacc agaccaaccc
     4381 ctcggccatc gtctccaaga agtcggcggg catcgaggcc ctcgaggacg tccagggcaa
     4441 gacgtggggc gccgttccgg gtggctcgcc gtacctgctc ctgaacgccc tgttccagga
     4501 gaacgacatc accgacgtgc gcgaggtcag cgtgccggcg ccgggcatcg cccagctcct
     4561 ggccgaccag gtcgacttca tcaccttctt cggcaacgag gtcgcgaaca tcgatcccaa
     4621 ccccgatgag aacctgaacg tggtcccgtt ctcggactac ggccaggaca tctacgggct
     4681 ggccatcgcc tctcaccccg actacatcgc cgagaacccc gaccaggtcg cgtgcttcgt
     4741 cgacggcgtg cggcgcgggt tcgaggcggc tcgcgaggat ccggaggcgg cgctcgacgc
     4801 gctcgaggcg gcgaacccgg agacggcgga gcgccgcgag gtgcatgcga agctgctcga
     4861 gggcgccttc gagtacgcgg gcgacgatct gctcgtgcag tcggaggaga agtgggccga
     4921 gacccaggac gtcctctcga gcgcggggat catcgagagc accgtcgagc catcggactt
     4981 cttcaccaac gactatcagt aggcaaccag tgagtgggcc gggtggcgcc gctggcgtcg
     5041 ctcggcccgg cacccatcga ggcacgtgac ggcatgacac ttctccagga ccgggtcgtc
     5101 ctcgtgacgg ggagcagccg cggcatcggt gccgaggtgg ccgttcgcgc ggccgccgtc
     5161 ggcgcgcgcg tcgccatcca ctgccactcg caccccgagg gcgcggagcg caccctgcaa
     5221 cgtgtgcgcg aggcgggcgg cgagggagcc gtcttccgcg ccgacctcgc cgacggcggc
     5281 gaggccgaag agctcgtggc gggcgtcatc ggccacttcg gacgaatcga cgggctggtg
     5341 aacaacgccg gccggaccca ggtcggcccg ttcctggacc tcaaacccca cgagtgggac
     5401 gcggtcatcg cgaccgacct gagcgccgcc ttccacacct cgcgcgccgc gctgccgagc
     5461 atggtcgaac gtgggagcgg cgccatcgtg aacgtcgcct cgcgcctggg ccagatgggc
     5521 atcacccaga cggcggcgta cagcgcggcg aaggccgggc tcatcggcct cacgcgctcg
     5581 ctcgcacgcg agttcgctcc gtacggggtg cgcatcaacg cggtcgctcc cggcttcacg
     5641 gtgaccgaga tgaccgccga cctggccgag accgagacgg gccggcagcg catgcgcgac
     5701 atcccgctcg gccggttcgg cgaggccggt gaggtcgccg atgcggtcgt cttcctcctg
     5761 tccgacgcgg cggcgctgtt cgtcggccag accctcaacc ccaacggtgg gagctacatg
     5821 ccgtgaccaa gatcatctcg gtccgggagg ccgtcgggaa ggtcgaggac ggcaagaccg
     5881 tcctgatcgg cggctccggg gccggccacg ccgttccgca gcgcttcatc gacgagctgg
     5941 cggcggtctt ccaggagcag ggacggccgc gtgacctgac caccgtccgc atcgtcggga
     6001 tcggcgactt cgccgatcgc ggcttcagcc agctcgggct gccgggcctc atgcgccgaa
     6061 ccatcggcag caacatcggc aacgagccgg ggctcggcgc cctggtggag gccggccagc
     6121 tggaggcgta ctcgttcccg cagggcgtgc tcagccagct gacccgcgag atcgccgcgg
     6181 ggcggccggg cctggtgacg caggtcgggc tcgggaccta cgtcgatccg cgccacaccg
     6241 gcggcaagca gaacgcggtg acgactgagg acttggtcga ggtcgtcacg ctgcgcggtc
     6301 gcgagtggct cttcttccac gccttcccca tcgacgtggc ggtgatccgc gggacgacgg
     6361 ccgacgagga cggcaacatc acgatggagg gagaggccgt ccagggcgag atgctggcga
     6421 tggccatggc ggcgcgcaac agcggcggca tcgtcatcgc gcaggtcaag cgcctcggcc
     6481 ggcgcggcag cctccccctg cgtgacgtca aggtgcccgg cgcgctcgtc gactacgtct
     6541 acgtggacga ccaccagtgg cagacctacg tcacgcagga ctcgccgtac tacgccgggg
     6601 cgttgcgcaa gcccgtcacg ccggagccgc cgcttccgct cgacgtgcgc aagatcatcg
     6661 cgcgccggtc gctcctggag ttcccgccag gggcgatctg caacctgggc ttcgggatca
     6721 gccagctgat cggccgcgtc gcctgggagg aggggatcac cgaccagctg accctgacgg
     6781 tcgagcaggg catcttcggt ggggtgccgg tcgccggcaa cgagggcggc gccggcttca
     6841 actaccaggc gatgatcgac cagccgtaca tgttcgactt ctacgacggc ggcgggctcg
     6901 acatcgcgtc gctctcgttc gccgaggtcg acgccgaggg caacgtcaac gtccacgcct
     6961 tcgagggccg cgtccgcggt cccggcggct tcccgaacat cagcgctcgc acgccgcgca
     7021 tcaacttcgt gggcacgctg actgcccagg gcctcgagct cgacatcgac gacggcgtgc
     7081 gcatcgtgtc cgaggggcgg ctctcgaagt tcgtgccgaa ggtccgggag atctccttca
     7141 acgggcacct cgcgcgcgag cgcggccagc aggtccggta catcaccgac cgcgcggtct
     7201 tcgccctgac cgatgacggc ctcgagctca tcgaggt
//
LOCUS       DASTYP010000200         5832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_673750, whole genome shotgun sequence.
ACCESSION   DASTYP010000200 DASTYP010000000
VERSION     DASTYP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673750"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /gene="vorB"
                     /locus_tag="VFV59_08855"
     CDS             complement(<1..1115)
                     /gene="vorB"
                     /locus_tag="VFV59_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEU5325974.1"
                     /translation="MTATTTATDSAAAALSPDERRGRTLMKGNEAIAEAAIQAGCEAY
                     FGYPITPQAELLEWMAKRMPELGRVFVQAESELAAINMALGAGATGARVLVSTSSPGM
                     SLMAEAMSYMAGSETPVVLVNVMRGGPGLGSIGPSQSDYLQATKGHGHGDYKVPVLAP
                     ASIAEAVELVPAAFELAERYRTPVILLADGIQGQAMEAVDLVFRNPERPAFDWALTGA
                     EGRAPRVVRSLHLHPEELEQHNTALQAKFARIAANEVRWSGERLEDADVVLVAYGTAA
                     RVAHSAMVLARDEGIRAGLFRPISLWPFPEAALRDLSAQVNGILVVELSAGQMIEDVR
                     LAVEGRCRIGFHGRTGGMVPTPAEVLTALRAQREEAAS"
     gene            complement(1112..1390)
                     /locus_tag="VFV59_08860"
     CDS             complement(1112..1390)
                     /locus_tag="VFV59_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5325975.1"
                     /translation="MTPVAELEPAASVAAAPWSPLLIAVDRCKGCELCISACPKHVLA
                     LDRSRVNALGYHPIELLDAAGCTSCAFCARVCPDAVFTVLAAPKGGPK"
     gene            1498..2439
                     /locus_tag="VFV59_08865"
     CDS             1498..2439
                     /locus_tag="VFV59_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5325976.1"
                     /translation="MQIVPFEPMHHEAAAALAAAAFRAGRRRNPLLPARWEQATTYLP
                     ELDAIQRHGSGVALVDGGRLRGYLVAFEVTRGDRRWTFTPEWAWAASGRGRTGVIQDL
                     YAAAADRWVADGSRTQHVSVHASDRVGLGAWSWLGFGHEVMDGIRDLTPIPTAATGVR
                     VRRARRSDAARLGDLEEGLRQHLAGTPVFFSLGAARTLEEQRRRIGRADAAVLLAEVG
                     GEIVGHLLVGPSSDDAATVIRDERTASIVGAFVRVEHRRAGVAETMLATAVAWARDGG
                     YERIAVDFETANLLATRFWTRYFRPVTYSLVRRLPAV"
     gene            complement(2436..2903)
                     /locus_tag="VFV59_08870"
     CDS             complement(2436..2903)
                     /locus_tag="VFV59_08870"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5325977.1"
                     /translation="MIDERHELLKTLRSTPLVVERLVRGLLDAQVRRRPSEGEWAIIE
                     VVAHLGDTDERTVERLRRMLAEDEPMLEPYDQAALAVERNYIGMDLAEQLGRFADVRA
                     EHVRLLAALDDAGWLRSGIHGEHGRITVQQLTAHSAGEDGDHLGQIARLVPGR"
     gene            complement(2900..3388)
                     /locus_tag="VFV59_08875"
     CDS             complement(2900..3388)
                     /locus_tag="VFV59_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5325978.1"
                     /translation="MTAPVRVIRRRPASAPLYLLDVSVERGDRTRAHGALWDGVLNAS
                     PEDTNDGPSPVEALLAAVAACFVRNLRWVADGSHVEFDRIDLHIAAERSDAPPAITAV
                     HVEVDLTTAAPGGRVAGIVERALKTGTITRTVARSTRFTLRLRVNAVEWPVEVESAMV
                     GR"
     gene            complement(3385..3561)
                     /locus_tag="VFV59_08880"
     CDS             complement(3385..3561)
                     /locus_tag="VFV59_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5325979.1"
                     /translation="MAAATGERENNVSNHLAKLRAGGLVRASRHQADARWVSYERDDD
                     ACARALAILGGLLT"
     gene            complement(3707..4066)
                     /locus_tag="VFV59_08885"
     CDS             complement(3707..4066)
                     /locus_tag="VFV59_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5325980.1"
                     /translation="MTGASGVGFIGFRTDRFDAMVALFRDRIGLEVIREAPGATWFRL
                     GTDAELHVYADTDRDHAFFTTGPVVGLRVADVEATRAALEADGLTMLTEVERTDSAAW
                     CHFRAPDGSVLEIIGPA"
     gene            complement(4063..5100)
                     /locus_tag="VFV59_08890"
     CDS             complement(4063..5100)
                     /locus_tag="VFV59_08890"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU5325981.1"
                     /translation="MSDSSWPIVGVAGARGVVGGVFCSILADAGLPAENLRPFGSGDG
                     NTLEYRGTSVPVTVLDDESARELDVLFLAVDGPQSREIMAGPGASVPLVVDNSSAFRL
                     ADDVPLVVPEANAHAARERSGNLIANPNCTTAAAVVALAPIRDVAGLASVDLASYQAV
                     SGAGRKGLDAFEAERGTEHRERADDSPFHGRIADSVVPQIDVLDETGWTGEEKKIHAE
                     LRKILELPDVDVAATTVRVPVHTGHSVALHIRTERDTTVEELEGALRAAPGLEYRPAD
                     GSERHPMPLDVEGRDPVLVGRLRAIPGRPRGFALWLSSDNLRKGAALNAIQSAAAADP
                     DRFGRLLPPAR"
     gene            complement(5097..>5832)
                     /locus_tag="VFV59_08895"
     CDS             complement(5097..>5832)
                     /locus_tag="VFV59_08895"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU5325982.1"
                     /translation="VELAGGLDGPLQWARAALDRGMPYVTANKALLATHGAELAELAE
                     RRAAALLGSASVGGGTPMIETVQHLAATGDIERVRGVVNATTTYILSAMGEGRSYADA
                     LREAQEAGYAEADPSFDVDGRDAAQKLAILASVAWGRWRPESDVETAGIRDLTVAAGD
                     TVRLVAEATPDQRRVAPTPVEGDDPVARAIGVESVLEVTVRDAGVFRVSGPGAGGRAT
                     AGAVYGDLARLVTGERPILFSAMVGR"
BASE COUNT          804 a         2107 c         2119 g          802 t
ORIGIN      
        1 gacgcggcct cctctcgctg tgcccggagc gccgtcagca cctccgcggg cgtcggcacc
       61 atccccccgg tgcgtccatg gaagccgatg cggcagcgtc cctccacggc caaccggacg
      121 tcctcgatca tctggcccgc cgacagctcc accacgagaa tgccgttgac ctgggccgag
      181 aggtcgcgga gcgccgcctc cgggaagggc cacaggctga tgggccggaa gagcccggca
      241 cggatgccct cgtcacgcgc gaggaccatg gcgctgtgcg cgacccgcgc cgccgtgccg
      301 tacgccacga ggacgacgtc cgcgtcctcg agccgctccc ccgaccaccg gacctcgttg
      361 gccgcgatcc gcgcgaactt ggcctggagg gccgtgttgt gctgctcgag ctcctcgggg
      421 tgcaggtgca gggagcggac gacgcgcggc gcgcggcctt cggccccggt cagggcccag
      481 tcgaaggccg gccgctcggg gttgcggaag acgaggtcca ccgcctccat ggcctggccc
      541 tggatgccgt cggccaggag gatgaccggc gtgcggtagc gctccgccag ctcgaacgcg
      601 gccggcacca gttcgacggc ctccgcgatg gacgccggcg cgagcaccgg gaccttgtag
      661 tcgccatgcc cgtggccctt cgtggcctgc aggtagtcgc tctgcgacgg accgatgctc
      721 cccagcccgg ggccgccacg catgacgttg accagcacga cgggggtctc ggagccggcc
      781 atgtagctca tcgcctcggc catcaggctc atgcccgggc tcgaggtgct gaccagcacc
      841 cgcgcgccgg tggcgccggc gcccagcgcc atgttgatcg ccgccagctc gctctcggcc
      901 tgtacgaaga cgcggccgag ctccggcatc cgcttcgcca tccactccag cagctcggcc
      961 tgcggggtga tgggatagcc gaagtacgcc tcgcagccgg cctggatggc ggcctcggca
     1021 atcgcctcgt tgcccttcat gagggtgcgg ccgcggcgct catccggcga gagcgctgcg
     1081 gccgcggaat cggtggctgt cgtggtcgcg gtcacttggg accgcccttg ggcgccgcca
     1141 ggacggtgaa taccgcatcg gggcagaccc gggcgcagaa ggcgcagctg gtgcacccgg
     1201 ccgcgtcgag cagctcgatc gggtgatacc cgagggcgtt gacgcgtgag cggtcgagcg
     1261 ccagcacgtg cttcgggcac gcggagatgc acagctcgca ccctttgcat cggtcgacgg
     1321 cgatgaggag cgggctccag ggagcggccg cgaccgaggc agccggctcc agctccgcga
     1381 cgggcgtcat gccgcgaacc tgcgcccggc acccaccccc gggtagggcc gaacggcccg
     1441 gcccgactgc tgcgatggtc accaggcgag tgcggcgggc gcgactagca tccgatcgtg
     1501 cagatcgtcc ccttcgagcc gatgcatcac gaggcggctg ccgcgctggc cgccgcggcg
     1561 ttccgcgctg ggcgtcggag gaatccgctg ctgccggccc ggtgggagca ggcaacgacc
     1621 tacctgccgg agctggacgc gatccagcgg cacggcagcg gcgttgcgct cgtggacggt
     1681 ggacggctgc gcggctacct cgtggcgttc gaggtgacgc gcggtgatcg tcgctggacc
     1741 ttcacgcccg agtgggcgtg ggcggcgtcg ggacggggcc gcaccggggt catccaggac
     1801 ctgtacgccg ccgccgcgga ccgatgggtg gccgacgggt cgcggacgca gcacgtcagc
     1861 gtccacgcca gcgaccgcgt gggcctcggc gcatggagct ggctgggctt cgggcacgag
     1921 gtgatggacg ggatccgcga cctgacgccg atccccacgg cggccaccgg cgtgcgcgtc
     1981 cgccgggccc ggcgctccga tgccgcgagg ctcggcgacc tggaggaggg gctgcgtcaa
     2041 cacctggccg gcacgccggt gttcttcagc ctcggggcgg cgcggacgct cgaggagcag
     2101 cgacgccgga tcgggcgcgc ggacgcggcg gtgctgctcg ccgaggtcgg tggcgagatc
     2161 gtcggccacc tgctcgtcgg cccgtcgtcg gacgatgccg cgacggtgat ccgcgacgaa
     2221 cggacggcga gcatcgtcgg ggcattcgtg cgggtggagc accgacgggc aggcgtcgcc
     2281 gagacgatgc tggcgacggc ggtcgcgtgg gctcgcgacg gcggctacga acgaatcgcg
     2341 gtcgacttcg agacggcgaa cctgctcgcc acgcgcttct ggacccgcta cttccggccg
     2401 gtgacgtaca gcctcgttcg gcgattgcca gccgtctagc gcccgggaac cagtctggcg
     2461 atctggccca ggtggtcgcc atcctcgcct gcgctgtggg cggtcagctg ctggacggtg
     2521 atcctgccgt gctcgccgtg gatgcccgac cgcagccagc cggcatcatc gagtgccgcc
     2581 agcaagcgca cgtgctccgc gcgaacgtcg gcgaagcgcc ccagctgctc ggcgagatcc
     2641 atgccgatgt agttgcgctc gaccgccagc gccgcctggt cgtacggctc gagcatcggc
     2701 tcatcctccg cgagcatccg ccggaggcgt tccaccgtcc gctcgtcggt gtcacccagg
     2761 tgggccacta cctcgatgat cgcccactcg ccctcggacg gacgtctgcg cacctgcgcg
     2821 tcaaggaggc cgcggaccag ccgctcgacc acgaggggcg tggagcgcag tgtcttcagg
     2881 agctcgtgtc tctcgtcgat catcggccga ccatagcgct ctccacctcg accggccact
     2941 ccacggcatt cacgcgcaac cgcagcgtga atctcgtaga ccgcgcgacg gtgcgagtga
     3001 tggtgccggt cttcagcgcc cgctcgacga tgccggcaac gcgcccgcct ggtgccgccg
     3061 tggtgagatc gacctcgacg tggaccgcag tgatcgccgg cggcgcgtcg ctccgctcgg
     3121 ctgcgatgtg caggtcgatg cggtcgaact cgacgtgcga gccatcggcc acccagcgga
     3181 ggttgcgcac gaagcaagcg gccacggctg ccagcagcgc ctcgaccggg ctcggtccgt
     3241 cgttggtgtc ctcgggcgag gcattgagca cgccatccca cagcgcaccg tgcgccctcg
     3301 tccgatcgcc gcgctcgacg ctgacgtcga gcaggtagag cggcgccgag gccggccggc
     3361 ggcggatgac gcggaccgga gccgtcatgt gaggagtcct ccgaggatcg cgagcgcccg
     3421 cgcacaggcg tcgtcgtcgc gctcgtagga cacccagcgc gcatcagcct gatggcgact
     3481 ggcgcgcacg agaccccctg cgcgcagctt cgccagatgg ttgctcacgt tgttctcccg
     3541 ctcaccggtg gcggcggcca tctcgcaccc acgggcgccg gacaagcgcg aggtaagcca
     3601 tcaacacgaa gaggtgtctc agtctgggct gagatgtccg gggccgaatg gcgtagatcg
     3661 accgtgctga gtgtcacctc gcgactccag ccagggtctt cggggtctac gcgggtccga
     3721 tgatctccag cacggagccg tccggcgcgc ggaagtggca ccacgcggcc gagtcggtgc
     3781 gctcgacctc ggtcagcatc gtcaggccat ccgcctcgag cgccgcacgc gtcgcctcga
     3841 cgtccgcgac acggagtccg accaccggcc cggtggtgaa gaaggcgtgg tcccggtccg
     3901 tatcggcgta gacgtgcagc tcggcgtccg tgccgagccg gaaccaggtg gcgccgggcg
     3961 cctcgcggat cacctcgagg ccgatgcggt cgcggaacag cgcgaccatg gcgtcgaacc
     4021 gatcggtgcg gaagccgatg aacccgacgc cggacgcgcc ggtcaccgag cgggcgggag
     4081 cagccgcccg aaacggtcgg gatcggcggc ggcagcactc tggatggcgt tcagcgccgc
     4141 gcccttgcgc aggttgtcgc tgctcagcca cagggcaaag ccgcgcgggc gcccggggat
     4201 ggcgcgcagg cggccgacga gcaccgggtc gcggccctcc acgtcgagcg gcatcgggtg
     4261 gcgctccgat ccgtccgccg gccggtactc caggccgggc gcggcccgca gcgcgccctc
     4321 cagctcctcg acggtcgtgt cgcgctcggt ccgaatgtgg agcgccaccg agtggccggt
     4381 gtggaccggg acgcggactg tggtggcggc cacgtccacg tccggcagct cgaggatctt
     4441 gcgcagctcg gcgtggatct tcttctcctc gccggtccat ccggtctcgt ccaggacgtc
     4501 gatctgcggc accacgctgt ccgcgatgcg gccgtggaac ggcgagtcgt cagcgcgctc
     4561 ccggtgctcg gtcccccgct ccgcctcgaa cgcgtcgagg cccttgcgac ccgcgccgct
     4621 gaccgcctgg taggacgcca ggtcgacgga cgccaggccc gcgacgtccc ggatcggcgc
     4681 cagggcgacg acggccgcgg cggtcgtgca gttcgggttg gcgatgaggt tgccggatcg
     4741 ctcgcgggcg gcgtgcgcgt tggcctcggg aacgacgagc ggaacgtcat cggccaggcg
     4801 gaaggcgctc gagttgtcga ccacgagcgg caccgatgca cccgggccgg ccatgatctc
     4861 gcggctctgc gggccatcga ccgccaggaa gagcacgtcc agctcgcgtg ccgactcgtc
     4921 gtcgagcacg gtcaccggca cggatgtccc gcggtactcg agcgtgttgc cgtcaccgct
     4981 gccgaagggg cggaggttct cggcgggcag gcccgcatcg gccaggatgg agcagaagac
     5041 gccaccgacg acgccacggg cacccgcgac accgacgatc ggccaggagg agtcgctcat
     5101 cgcccgacca tagccgagaa gaggatgggg cgctccccgg tcaccagccg cgcgaggtca
     5161 ccgtacacgg caccggccgt ggcccgtccg cccgcgccgg gtcccgagac gcggaagacg
     5221 ccggcgtcac ggacggtgac ttcgagaacg ctctcgacgc cgatggctcg ggcaacgggg
     5281 tcgtcgccct cgacgggcgt gggcgccacg cgacgctggt cgggggtcgc ttccgccacc
     5341 aggcgcaccg tgtcgccggc tgccacggtg agatcgcgga tgcccgccgt ctcgacatcg
     5401 gactccggcc gccagcggcc ccaggccacg gacgccagga tggcgagctt ctgggccgcg
     5461 tcgcggccgt cgacgtcgaa gctcggatcg gcctcggcgt agccggcctc ctgcgcctcg
     5521 cgcagtgcat cggcgtagct gcggccctcg cccatggccg agaggatgta ggtggtggtg
     5581 gcgttcacga cgccacgcac ccgctcgatg tcgccggtcg ccgccaggtg ctggaccgtc
     5641 tcgatcatcg gcgtgccgcc cccgaccgat gcgctcccca gcagcgccgc ggcgcgtcgc
     5701 tcggccagct ccgccagctc ggccccgtgc gtcgccaaga gggctttgtt cgccgtcacg
     5761 tacggcatcc cgcgatccag cgcggcacga gcccactgca gcgggccatc gaggccaccg
     5821 gcgagctcca cc
//
LOCUS       DASTYP010000201         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1292567, whole genome shotgun sequence.
ACCESSION   DASTYP010000201 DASTYP010000000
VERSION     DASTYP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292567"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFV59_08900"
     CDS             complement(<1..604)
                     /locus_tag="VFV59_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5325983.1"
                     /translation="MLERAVEAGLERILVPGWDVASSEAALELSARHPGLVDAAVGVH
                     PHHARHMDEAAWTRLEGLVGDPRTRAIGEIGIDHFRNLSPPDVQRAVFDRQLELAGRH
                     GLAVLVHDRDAHAEVTQALVAWARTSGRGTPGVIHAFSGDVAMAHRLIAAGFLVSFAL
                     PLTFRTAVGPRAAAPSLEAGTFLVETDSPYLGPDRDRVNEP"
     gene            complement(694..2256)
                     /gene="metG"
                     /locus_tag="VFV59_08905"
     CDS             complement(694..2256)
                     /gene="metG"
                     /locus_tag="VFV59_08905"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5325984.1"
                     /translation="MPDRFYLTTAIFYPSPRPALHSIFEAIGADMIARYHRLLGDDTR
                     FLTGLDEHSANVERDAREQGVDPHTLIDPWAETWERTFERYLISADRFIRTTDPDHAD
                     AAAEMVRRAQANGDVYQGVYSGWFCTGCNEFKTEQQLVDGRCPEHPTLDPQWLEESNY
                     FFRLSAYQERLERLYAENPAFCEPEHFRNEVLGWLREGLRDFSISRAGGTWGIPFPGD
                     PSHRIYVWFDALVNYLTGAGFPNHPDEVERWWPADLHVIGKNITRFHCLYWPAMLMSA
                     GLPLPKQVFAHGFMLERGEKMSKTRGNVRDAWETADTMGVDGVRYAVLREVPFDRDAD
                     VTWDGFVRRYNADLANDLGNLVNRTISMATRYLGGELPPVTDAGAAADLELRAAAERS
                     VAAYRAAMGRHHLDEALAAMMEVAGAANGYAESQAPWALNKAGERERVGQVLACMAEA
                     CRIVGHLLAPVAPVGARHIHEQLGVPAPYDERGAGGPGLEALVRWGAGPSSWRTGSAS
                     PIFPRLELEATA"
     gene            2312..3112
                     /locus_tag="VFV59_08910"
     CDS             2312..3112
                     /locus_tag="VFV59_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5325985.1"
                     /translation="MEILTEDRREISLESEHYNARLVRRVDETDDLGYFWVKFDGEPI
                     EFSPGQYMTIGVYADDKLVQRPYSVASAPRESGDGGYEFYVRLVPILRFTTLLWRLPI
                     GHGMRMIGPKGKFVLEPDDDRTHLYVSTGTGIAPFMSMIRDTMDRPRRTVMLHGCSYA
                     DELGYRDILEGWERGGGYPVQYVPTISRPGDPRNAAWSGRTGRVEQVVASVCEDLGLQ
                     PDRTVVYICGNPEMILNVEAVLMERGFPEFHVKKELYWPKGKQVTTVG"
BASE COUNT          483 a         1159 c         1172 g          477 t
ORIGIN      
        1 tcggctcgtt gacgcggtcc cggtcggggc cgaggtaggg cgagtcggtc tcgaccagga
       61 acgtgccggc ctcgagcgac ggcgccgcag cccgcggacc gacggcagtg cggaacgtca
      121 gcggcagggc gaacgacacc aggaagccgg cggcgatcag ccgatgcgcc atcgccacgt
      181 ctccggaaaa ggcatggatc acgcccggcg tgccgcgtcc cgaggtccga gcccacgcga
      241 ccagggcctg cgtgacctcc gcgtgcgcgt cacgatcgtg gacgagcacc gccagcccgt
      301 gccggcccgc cagctccagc tgccggtcga acacggcgcg ctggacgtcg ggcggcgaga
      361 ggttgcggaa gtgatcgatc ccgatctcgc cgatcgcgcg cgtccgcggg tcgcccacga
      421 gcccctccag gcgcgtccac gcggcctcgt ccatgtgacg agcgtggtgc ggatgcacgc
      481 cgacggccgc gtcgacgagg ccgggatggc gggcgctgag ctcgagcgcc gcctctgatg
      541 aggcgacgtc ccagccgggc accaggatcc gctccaggcc cgcctcgacg gcccgctcga
      601 gcacgggctc gaccgcgccc gcgaatcgct cgtgctggag atgggcatgc gagtcgacga
      661 gccccgggag tcgccgaggg ccgcggcggc ggctcaggcg gtcgcctcca gctcgagccg
      721 cgggaagatc gggctggcac tgcccgtccg ccaggacgac ggaccagcgc cccaacggac
      781 cagcgcctcg agcccgggcc cgccggcgcc gcgctcgtcg tacggcgccg gcacgcccag
      841 ctgctcgtgg atgtggcgtg cgcccacggg cgccacgggc gcaagcagat gcccgacgat
      901 gcggcacgct tccgccatgc acgccagcac ctggcccacg cgctctcgct cccccgcctt
      961 gttgagcgcc cacggcgcct gcgactccgc gtagccgttc gcggcgcccg cgacctccat
     1021 catcgcggcc agcgcctcgt cgaggtggtg ccgccccatg gcggcccgat aggccgccac
     1081 cgagcgctcg gcggcggcac gcagctccag gtcggccgcg gcgcccgcat cggtcaccgg
     1141 cggaagctcg ccacccaggt agcgcgtcgc catgctgatg gtgcggttga ccaggttgcc
     1201 gaggtcgttg gccaggtcgg cgttgtatcg gcgcacgaag ccgtcccagg tgacgtcggc
     1261 gtcgcggtcg aacgggacct cgcgcaggac ggcgtagcgg acgccgtcga cgcccatggt
     1321 gtccgccgtc tcccacgcgt cgcgcacgtt gccgcgcgtc ttgctcatct tttcgccgcg
     1381 ctcgagcatg aagccgtgcg cgaagacctg cttcggcagc ggcaggcccg cgctcatgag
     1441 catggccggc cagtagaggc agtggaagcg cgtgatgttc ttgccgatca cgtgcaggtc
     1501 cgccggccac cagcgctcga cctcgtcggg atggttgggg aagcccgcgc cggtgaggta
     1561 gttcaccagc gcgtcgaacc agacgtagat ccggtgcgac ggatcgcccg ggaacggaat
     1621 gccccacgtg ccaccggccc ggctgatgct gaagtcccgc agcccctcgc gcagccagcc
     1681 gaggacctcg ttgcggaagt gttccggctc gcagaacgcg gggttctccg catacaggcg
     1741 ctcgaggcgc tcctggtagg ccgagagacg gaagaagtag ttcgactcct ccagccactg
     1801 cggatcgagg gtcgggtgct cgggacatcg cccgtccacc agctgctgtt cggtcttgaa
     1861 ctcgttgcag ccggtgcaga accagcccga gtacacgccc tggtagacat cgccgttggc
     1921 ctgggcgcgt cggaccatct cggcggccgc gtcggcgtgg tcgggatccg tcgtgcggat
     1981 gaaccgatcc gcgctgatca ggtaacgctc gaaggtccgt tcccaggtct cggcccacgg
     2041 gtcgatcagc gtgtgcgggt ccactccctg ttcccgcgcg tcgcgctcca cgttggcgga
     2101 gtgctcgtcg agaccggtca ggaaccgcgt gtcgtcgccc agcaggcggt ggtagcgcgc
     2161 gatcatgtcg gcgccgatgg cctcgaagat cgagtgcagc gccggacgcg ggctggggta
     2221 gaagatcgcg gtggtcaggt agaagcggtc gggcatcggg acggcgattg tagcgaccgg
     2281 acgtccgggg ccggtgacta cactgatcgg cgtggagatc ctcaccgagg accgacgcga
     2341 gatctcgctg gagtccgagc attacaacgc gcgcctcgtg cgccgcgtgg acgagaccga
     2401 tgacctcggc tacttctggg tcaagttcga cggcgagccg atcgagttct cacccggcca
     2461 gtacatgacc atcggcgtct acgccgatga caagctggtc cagcgcccct attcggtcgc
     2521 ctcggcgccg cgcgagtctg gcgacggcgg ttacgagttc tacgtccgcc tcgtgccgat
     2581 cctgcgcttc acaaccctgc tgtggcggct gccgatcggc cacggcatgc ggatgatcgg
     2641 tccgaagggc aagttcgtgc tggagccgga cgacgaccgg acgcacctct acgtgtcgac
     2701 cgggacgggc atcgcgccgt tcatgtcgat gatccgcgac accatggacc gcccgcgccg
     2761 gacggtcatg ctgcacggct gctcgtacgc cgacgagctc ggctaccgcg acatcctgga
     2821 gggctgggag cgcggtggcg gctaccccgt ccagtacgtc ccgacgatct cgcgccccgg
     2881 cgacccgcgc aacgccgcct ggagcggtcg caccggccgc gtggagcagg tggtcgcgtc
     2941 ggtgtgcgag gacctggggc tgcagcccga ccgcaccgtc gtgtacatct gcggcaatcc
     3001 cgagatgatc ctgaacgtcg aggcggtcct catggagcgc ggcttcccgg agttccacgt
     3061 caagaaggag ctctactggc cgaaggggaa gcaggtcacc acggtcggct gacctgcagc
     3121 gcggctgccg cgccgcgttg tcccggcagt gcccgggcca aagggcccgc cagttggggc
     3181 ccactgaccg atgggatcgc gcgccggcgc gcggagcatc ggctcatgca tcgcatcgtc
     3241 gctcgcacgc agctgtctcc gaacgtgacc cggctggtgg tggaggcgcc g
//
LOCUS       DASTYP010000202         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1475061, whole genome shotgun sequence.
ACCESSION   DASTYP010000202 DASTYP010000000
VERSION     DASTYP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475061"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /gene="fabD"
                     /locus_tag="VFV59_08915"
     CDS             <1..994
                     /gene="fabD"
                     /locus_tag="VFV59_08915"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5325986.1"
                     /translation="NVTQPTDAPDAPRARTIAFVFPGQGSQYVGMGADLIERSPAAAA
                     AFERADQALGIPLTRLILEGPAEELDQTVNAQPAILATSVAYLDAMREEARQAGIDLV
                     PMAVAGHSAGQYAAAVAANALDYTDALRLVRERGRIMQEKGIAGGMGAVIGLSDEQVD
                     EVVEQAREHGEISVANANAPGQIVLSGVIPALVFALEMSRTVGARKAVRLTVSVASHS
                     PLMRRARDEFGRILARVPFRDPQIPMLGNVHASFIDTADGLRLELREHLVHGVQWTAT
                     VRRMAGSGVTDFVEVGPGRVLSGLIRRITPDAVTHQLDGVNPAAWLQSLRESPA"
     gene            991..2268
                     /gene="fabF"
                     /locus_tag="VFV59_08920"
     CDS             991..2268
                     /gene="fabF"
                     /locus_tag="VFV59_08920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5325987.1"
                     /translation="MTRRRAVITGYGVLSPVGIGADATWDALVSGRSGIDEITLFDSS
                     PFEVKVAGEVKGFDATQYMDAKDVRRHDRCTHFAVAATGEALRHAGLLGDDGRVSDVD
                     RDRFGMVFGTGIGGIGMLLEGQMTLVERGPRRVSPFLLPHMLPDSPSGQVAIQYGIRG
                     PNMAVVSACATGGHAIGEAMETIVRGQADIMLGAGAEAPLVPLAFAGFGQMRALGTPK
                     DPDTGEYDPRMASRPFDATRDGFVVAEGSAVLVLEELEHARARGARPIAEVIGYGSSA
                     DAYDMAAPAERGEGNQRAMRAALARAGIGPEAVDYINAHGTSTPLNDRYETMAIRDVF
                     GEHADKLAVSSTKSMTGHAMGAAGAIEGFACVKVLETGCIPPTINYRHPDPELTLDYV
                     PNEARAADVRVALSNSMGLGGHNSTVILRRFDE"
BASE COUNT          341 a          868 c          812 g          312 t
ORIGIN      
        1 caacgtgacg cagccaaccg acgcgccgga cgctccgcgc gcgcgcacga tcgccttcgt
       61 cttccccggc cagggctcgc agtacgtcgg catgggcgcc gacctcatcg agcgctcgcc
      121 ggcggccgcg gctgccttcg aacgcgccga ccaggcgttg ggcatccccc tcacccgcct
      181 catcctcgag ggaccggccg aggagctcga ccagacggtc aacgcccagc cggcgatcct
      241 ggcgaccagc gtcgcgtatc tcgacgccat gcgcgaggag gcacgtcagg ctggcatcga
      301 cctggtgccg atggcggtgg ccggccattc cgccggccag tacgccgccg ccgtcgccgc
      361 caacgccctc gactacaccg atgcgctgcg cctcgtccgc gagcgcggcc ggatcatgca
      421 ggagaagggc atcgccggtg gcatgggcgc ggtcatcggc ctctccgacg agcaggtgga
      481 cgaggtcgtc gagcaggcgc gcgagcatgg cgagatctcg gtggccaacg cgaacgctcc
      541 cggccagatc gtcctgtccg gcgtcatccc ggccctggtc ttcgcgctcg agatgagcag
      601 gacggtcggc gcccgcaagg cggtgcgact caccgtcagc gtggcgagcc actcacccct
      661 gatgcgtcgc gcccgggacg agttcgggcg catcctggcc cgcgtcccct tccgcgatcc
      721 ccagatcccg atgctcggca acgtccacgc cagcttcatc gacaccgccg acggcctccg
      781 tctcgagctg cgcgagcacc tcgtccacgg cgtgcagtgg accgccaccg tgcgccgcat
      841 ggcgggctcc ggtgtgacgg acttcgtcga ggtcggcccg ggccgcgtgc tctcgggcct
      901 catccgccgc atcacgccgg acgccgtcac gcaccagctc gatggcgtga atccggccgc
      961 ctggctgcag agcctgcgcg agagccccgc gtgacccgcc gccgcgcggt catcaccggc
     1021 tacggcgtcc tctcgccggt cgggatcggc gccgatgcca cctgggacgc gctcgtgtct
     1081 ggccgctcag gcatcgacga gatcacgctg ttcgactcgt cgccgttcga ggtgaaggtc
     1141 gcgggcgagg tcaagggctt cgacgcgact cagtacatgg atgccaagga cgtgcgccgc
     1201 cacgaccgat gcacccactt cgccgtcgcc gccaccggcg aggcgctgcg ccacgccggc
     1261 ctcctcggcg acgacggccg cgtcagcgac gtggaccgcg accggttcgg catggtgttc
     1321 gggaccggca tcggcgggat cgggatgctg ctggagggcc agatgacgct cgtcgaacgc
     1381 ggtccccggc gcgtctcccc gttcctgctg ccccacatgc tgccggactc gccctcgggc
     1441 caggtcgcga tccagtacgg catccgtggc ccgaacatgg cggtcgtcag cgcctgcgcg
     1501 accggcggcc atgccatcgg tgaggcgatg gagaccatcg tgcgcggcca ggccgacatc
     1561 atgctcgggg ccggtgcgga ggcgccgctg gtgccactgg ccttcgccgg cttcggacag
     1621 atgcgggcgc tcgggacgcc gaaggacccg gacaccggcg agtacgaccc gaggatggcc
     1681 tcccggccct tcgacgcgac gcgcgacggg ttcgtggtgg ccgagggttc ggcggtgctg
     1741 gtcctcgagg agctcgagca cgcgcgggcg cgcggggcac ggccgatcgc cgaggtcatc
     1801 ggctacggct cgtccgccga cgcgtacgac atggccgccc cggccgaacg cggcgagggc
     1861 aaccagcggg cgatgcgggc cgcgctggca cgcgccggca tcggtcctga agcggtcgac
     1921 tacatcaacg cgcacggcac gagcacgccg ctgaacgacc gatacgagac gatggcgatc
     1981 cgcgatgtct tcggcgagca cgcggacaag ctggccgtct cgagcacgaa gtcgatgacc
     2041 ggccacgcca tgggtgccgc cggcgcgatc gagggctttg cgtgcgtcaa ggtcctggag
     2101 accggctgca tcccgccgac gatcaactac cgccatccgg atcccgagct gaccctcgac
     2161 tacgtgccga acgaggcgcg ggccgcggac gtccgcgtcg ccctctcgaa ctcgatgggc
     2221 ctgggcggcc acaacagcac ggtgatcctg cgccgcttcg acgagtaggg ccgagcgccc
     2281 accgagggga gagccgccac catgacgacc acccgagcat cccgagaccc cgg
//
LOCUS       DASTYP010000203        17085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1000078, whole genome shotgun sequence.
ACCESSION   DASTYP010000203 DASTYP010000000
VERSION     DASTYP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 17085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17085
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000078"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..1841
                     /locus_tag="VFV59_08925"
     CDS             525..1841
                     /locus_tag="VFV59_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU5325988.1"
                     /translation="MQKYMRWSAILVVLMLVLAACQGGGGSSAGESAGESEPAASQPE
                     ASEGGGGGTGSAADCEADEFGCIELAEGDPLVIGTALVISGANEALGTDSQWGVEVAA
                     QFRPEIAGHEVEFNHQDDGCSAEGGTAAARALVSEENIAAVIGTSCSGAGVPAAEITS
                     AEGILMVSPSNTAPSLTAPETHEPFYARTAHNDNIQGAAMAQFACEVLGVGSAATIDD
                     GSPYADQLANVFARQFEEQCDGTVTAEEAVTVGQTDFSGVLENIAADSPELLYFPIFV
                     AEGALITQQAKDTAGLEDTILAGADGYFTPDWLEATGDAAEGHYLSGPDLAFSGNFYE
                     DEFLPAYREISGQDEPISVFHAHAFDAYNMIADAIEAVAFTDGGTTYIPRTALRDAFF
                     STSGYEGVTGTLTCDENGDCADAKISVSQVQDVDGELQFVRVWPEG"
     gene            1940..2992
                     /locus_tag="VFV59_08930"
     CDS             1940..2992
                     /locus_tag="VFV59_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU5325989.1"
                     /translation="MPGRPPRLNLVNLFLWLLGGGILVLAGVGAFLTLSQGRLSGDQW
                     ESLIVAGIALGSVYALIALGYTLVYGILFMINFAHGEVFMWGAFTAYFAATALDDAGM
                     MDANPILALFLTLLTAMLVSMVIAIILERVAYRPLRGAPRLVPLITAIGASLFLQYTA
                     RGFYGSGVRAYPSYGGLFEGTIDIGPLTMPIEDAVVVVAALALMLALYFFINNTRTGR
                     AMRAVSEDKDVAAMMGINVDRIIVTTFAIGGLLAGAAGILYTLLFNQVHFFMGFLPGI
                     KAFTAAVLGGIGNIIGAALGGLTLGILEQLGPNLFLTGYNIPAPNQLKDVLAFTVLVL
                     VLIFRPSGFLGRKENV"
     gene            2992..4704
                     /locus_tag="VFV59_08935"
     CDS             2992..4704
                     /locus_tag="VFV59_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine/isoleucine/valine transporter permease
                     subunit"
                     /protein_id="HEU5325990.1"
                     /translation="MPPLLRRAVITGFLGGAAGIHVSLVGMVEDFQGRDTIADIVSVG
                     TMLPILIAIVVGWSAGSRMRAPLIDPTPRESLLFGTLAGAVTGALMALFALFINTVDV
                     AWILSNAKPSLADLLWLDQGPVVGSLLLIIGGALFGAVGAVLHVLPGLISRSLAIGGA
                     VTIVVALMEPFLGAVLRNVGLRFLEGFLYASGGLTLPGFILVFALVAGGVYLWRTRGH
                     GTKERVEAMPADRRRIGKIVLYLAFIGLLLLLPQIVGSRLSEVVGTIGLYVLLGLGLN
                     IVVGFAGLLDLGYVAFYAVGAYTTAVLTSPASPLISPELPFWGALPFVIVAAAMIGLM
                     VGTPVLRLRGDYLAIVTLGFGEIARLIFLSEWAKPIVGGAQGILSIPPPLPFTNHPQV
                     IYYPILFFCILAAIAASRLAASRVGRAWNAMREDESVAEATGVNTTKYKLLAFGLGAA
                     FGCLSGAFFAAKIGTIFPNSFEILVSINALALIILGGMGNIYGVVVGAFVLVGLPELL
                     REFAEYRLLIYGVVLVVMMLLRPEGLLPSRSRRAELHEDQGDDEAMYEHEAGVDTGRP
                     VVTG"
     gene            4710..5540
                     /locus_tag="VFV59_08940"
     CDS             4710..5540
                     /locus_tag="VFV59_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325991.1"
                     /translation="MTETTAHSTPSTATAPEAGDLLRAEQVTMRFGGLIANKDVDFTI
                     PRGSIVSLIGPNGAGKTTFFNQLTGMLQPTSGAITFDGTSIIGLSPHEIVELGVGRTF
                     QNIRLFGDMTVLANVMVGRHVRMKSAWWQSLLGLPSVRAEEEAVRQRARELLDMVGLR
                     RGYEEEFAKNLPYGDQRRLELARALASDPKLLLLDEPTAGMNPGETARMTQLIRRLRD
                     ELQLTVLLIEHDMKVVMGISERITVLDHGELLAEGHPDEIRSNPQVIEAYLGKGAAGT
                     "
     gene            5537..6325
                     /locus_tag="VFV59_08945"
     CDS             5537..6325
                     /locus_tag="VFV59_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5325992.1"
                     /translation="MSDQARTDPVATATASTAPAADASGTAPLLELDDLHVFYGNIEA
                     LKGVSISVGRGEVVTLIGSNGAGKSTTLRAISGTHKPRQGSIRLGGRPIHQLPAYEVV
                     KLGIAHSPEGRKVFSQMTVFENLQMGAFSRRGEKIDDDVARVFDLFPRLKERRGQKAG
                     TMSGGEQQMLAMGRALMARPSILLLDEPSMGLSPILTEQIFRIITEINEQGTTVLLVE
                     QNALMALNVSSRGYVIQTGKIILADTAANLAANEQVRQAYLGEI"
     gene            6339..6491
                     /locus_tag="VFV59_08950"
     CDS             6339..6491
                     /locus_tag="VFV59_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325993.1"
                     /translation="MAEEPQPPPSRPKGPGDYRPDLRPMLILGVLLVAVVLAWILLSP
                     LILPST"
     gene            complement(6488..6916)
                     /locus_tag="VFV59_08955"
     CDS             complement(6488..6916)
                     /locus_tag="VFV59_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325994.1"
                     /translation="MDGGGRRFRLLVLLGSLAMTGSGFVPWWRSGGESVSGVQVPAAQ
                     GIGLEGPGLVIYGVAILALVLLDVGYMRGRWGFFLDSPWTYLVLGLVGGAALAYRGWE
                     LWSAGYLPLPQQSPGLALAAVGVALLLYGAGAGFGAPRRY"
     gene            complement(6920..7474)
                     /gene="hpt"
                     /locus_tag="VFV59_08960"
     CDS             complement(6920..7474)
                     /gene="hpt"
                     /locus_tag="VFV59_08960"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5325995.1"
                     /translation="MHDDVEEILLDRATVAARVSELGAQLATDYADRDPVLVSVLKGS
                     LIFLADLMRAMEIPVSVDLMEVSSYGSGTETSGQVRILKDLSGPIEGRHVIVVEDIID
                     TGLTLNYLLKYLADKQPASVEIVCLLDKPARRLAEIPIAYRGFTIPDRFVIGYGLDYD
                     ERYRNLPYIGVLKPSVYGGAPPEG"
     gene            complement(7477..7911)
                     /locus_tag="VFV59_08965"
     CDS             complement(7477..7911)
                     /locus_tag="VFV59_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5325996.1"
                     /translation="MTSDSPPRFAHESEAELARILDFYGVAWRYEPDVFPISWNADGA
                     VTESFAPDFYLPEIDLYVELTTLKQSLVRKKNRKLRWMRQLYPEVRVKLFYARDFKAL
                     MLKYGRLDFLTDVTAANGSTHGPNGTGNGNAAANGAVAVAAR"
     gene            8190..8951
                     /gene="rsmG"
                     /locus_tag="VFV59_08970"
     CDS             8190..8951
                     /gene="rsmG"
                     /locus_tag="VFV59_08970"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU5325997.1"
                     /translation="MSATPSRTVARAARDALEQLLATDAPALRRQLPPDFLDRAERYV
                     ALLLETNARMNLTRVVDPDAVARLHLLDALAALGLIDAVAPSLALDLGSGGGVPGLVL
                     AIARPNIRWVLVDSVRKKIDALRTFAAALGLRNVEVLAERAEILGRDPERRDRADLVT
                     ARACASLPVLLEYALPLLRVDGRLVAWKGPIGDDELSAGGRAAGELGGGVPWVEPTGV
                     EALGEHRFVLVEKVRPTPARFPRRPGEAARRPLGG"
     regulatory      9029..9091
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            9119..9493
                     /locus_tag="VFV59_08975"
     CDS             9119..9493
                     /locus_tag="VFV59_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEU5325998.1"
                     /translation="MLSLLLVGLGGFAGAVARYIVDARVSAWTGGTLPWGTLAVNVTG
                     SFLLGLLFALVIERAALPAGLRAPLMIGFIGSYTTFSTLALESWRMIEDGAWLTAGAN
                     LVGSILIGVVAVVAGVAIGRSM"
     gene            9490..9834
                     /locus_tag="VFV59_08980"
     CDS             9490..9834
                     /locus_tag="VFV59_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU5325999.1"
                     /translation="MTKLEGTGLLVRIYLGESDRWHGRPLYQAIVERLRERGLAGATV
                     LRGIEGFGAKQRLHSTRILSLSEDLPILIEAVDTEEKIRATLPELDEMVSDGLITIER
                     VEVITYRASPST"
     gene            9902..10636
                     /locus_tag="VFV59_08985"
     CDS             9902..10636
                     /locus_tag="VFV59_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU5326000.1"
                     /translation="MRIALLSDIHANLAALEAVVADLPAVDEVWVLGDTVGYGPQPNE
                     VIAMLQAMGARSVLGNHDGAAIGCVDADPFNPDARAAIDWTTTVLDENSRAYIATLPE
                     VRVDRAMTAVHGSPRDPIWEYITSASIAAANFSAFSSRLCLFGHTHLPAAYRAVDGRV
                     EGVPGMPGDVVRLHGARAMLNPGSVGQPRDGLPAAAYAILETNGDDEADAIEFRRVRY
                     DVDRTQRIMREVGLPPRLAERLSYGR"
     gene            10697..11188
                     /locus_tag="VFV59_08990"
     CDS             10697..11188
                     /locus_tag="VFV59_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5326001.1"
                     /translation="MDFFRRSEKRPSHFPYRRLLVPLLGDPDVDDPALRLAVILLSGR
                     EEAEVALLHVIEVPFERNLDAEDQRAIEAADEILARGEHLLTEHEIRTRTSTVQARAA
                     GPAIVDDANELGAELIVMGLRYKKRFGGQWDAGRTVPYVMRNSTAPVWCLRAETKELA
                     HTP"
     gene            11185..11784
                     /locus_tag="VFV59_08995"
     CDS             11185..11784
                     /locus_tag="VFV59_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5326002.1"
                     /translation="MRVIIVGCGRVGARVAAELDERGENVTVIDVIQRAFNRLPQGFG
                     GETVRGNGTDEDIMRSAGAEQADIVMALTEGDNRNALAAQLAKHTFGVPRVIVKINDP
                     VRAEAYRTLGLETICRTLMLGDALVTAAMEGAEATNGFVEAPTAEPLRGAPVPGSLAD
                     AEARAAMAADVDATARSVEPTADEPSGKVLPQGGVEGGM"
     gene            11785..12444
                     /locus_tag="VFV59_09000"
     CDS             11785..12444
                     /locus_tag="VFV59_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5326003.1"
                     /translation="MYVVVIGGGNVGYYLTKELLAAGHEVVVIEEDQHRARQIADELG
                     SIVIPNDGCEGRYQAEAGMARADVMAAVTGDDAVNLAACQIAKMRFNVPRSIARINDP
                     KNEKLFRQLAIDEVVSPTRSILGVIEHEIPIHDLLHLAELEGGEVQIVEAQLDGDSPA
                     IGRELREIELPEGSNIAVLIRDDRAMPARSDVRLRAGDKVLAVTSAEDEAELRSLLIG
                     E"
     gene            12441..13184
                     /locus_tag="VFV59_09005"
     CDS             12441..13184
                     /locus_tag="VFV59_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326004.1"
                     /translation="MTGPDPGRVLRLALLAWGLGHLAIGRRALGRGLLIAEIAGIGLV
                     AWLTASLADSSAYLLPFLAGITFLAVWAAQAIAAYREAHALQAARAPTPARSPAIAIG
                     WLSLPLLAWATGFWLIGARDANPSAVLDRFVTAWSTDGLDGAGWPRAVVGAANRAARD
                     LGTDADRFRDVRVRIVEQDDRRATAVAEAIHFERRATTLLGLFPGTELVPVADELVLE
                     LELRASPVELAGGGDIGAVHWELVAADAP"
     gene            13349..13825
                     /locus_tag="VFV59_09010"
     CDS             13349..13825
                     /locus_tag="VFV59_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5326005.1"
                     /translation="MANITRYDPLGEMVSLRQAMDRLFEDSFVSPLSWRTISGGESIT
                     PAIDVHETADDVVITAALPGVKPEDVDITMTGQSLTLKGEFKADESIDRDQYLYRERR
                     YGTFTRSLQLPVRVKGEGAEATFADGILTLRVPKAEEVKPRQIRINASGNGGQTQP"
     gene            13827..14213
                     /locus_tag="VFV59_09015"
     CDS             13827..14213
                     /locus_tag="VFV59_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5326006.1"
                     /translation="MDRSRRPAPHHGVPRRDDLTRPRYIISIAAELASCHPRTLRIYE
                     EEGLLAPQRRNNLRLYSEADIQRVRIIRFLTRRQRVNLAGVRVILQLEDLGKIRVYDL
                     FDEDDIERFAENDPDQPAEASAEGAT"
     gene            14214..16748
                     /gene="lon"
                     /locus_tag="VFV59_09020"
     CDS             14214..16748
                     /gene="lon"
                     /locus_tag="VFV59_09020"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5326007.1"
                     /translation="MATEDTRENNEMEATAEDATAAERIQVVPLVALRDTVIFPEMIV
                     PLQVGRDRSVKALDRAVRTSQPVALVTQRVNDQEEINAAEELYAVGTLAKIAQVIRLQ
                     DGTVRAIVQGQKRIRLLDLLQTDPYLEARIEAVDDSSEKTLEIEALMASIHSQIEQYV
                     NSGASVPPEVAVAARNITDGGLLADMTAYSPEMTTEQRQELLETIDVPDRLRLASSFL
                     AKQIEVLELKGRIQSEVKSEMDKTQREYILREQMKAIQKELGEDDPAVAEANELRERV
                     EQSAMPEEVKEKALKEVDRLSRIPSASPEQGVIRTYVDWLVSLPWGVETDDNLDLEEV
                     ERVLNEDHWGLEKIKERIIEYMAVRKLAEKIRSPILLFVGPPGVGKTSLGKSIARAMG
                     RKFVRMSLGGIHDEAEIRGHRRTYIGALPGRVIQNIKTAGSANPVFMLDEIDKVGMDF
                     RGDPSSALLEVLDPEQNFSFQDNYLEVPFDLSKVLFIATANMLDTIPPALRDRMEVIQ
                     LPGYTQLERVRIAERFLVPKQLEAHGLKDDQLVFEEPTLVRLIQAFTREAGVRNLERE
                     IANVARKVARRVATDADAKVTIHPEDLEEYLGPARFEYGELEAEDQIGMVTGLVVSDA
                     GGDIVQVEATRMEGKDEFILTGQLGSVMQESARAGMSYIRARTKELGIDPTAFEKTTV
                     HIHVPAGATPKDGPSAGVTMATAMASLFTGKPVRRDLAMTGEITLRGRVLPIGGLKQK
                     LLAAHLAGVKTVLIPMRNEKDLVDVPDEVREQLRIVPVENMDQVLGEALIDAPRSAAR
                     IKAERDERQKRAARRPRRSRKPATEEPPIAATGDVEQPQPPAGQPA"
     gene            16952..>17085
                     /locus_tag="VFV59_09025"
     CDS             16952..>17085
                     /locus_tag="VFV59_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU5326008.1"
                     /translation="MDYKDYYAILGVPKTATQAEIKKAYRKLARELHPDTNKDPDAEK
                     R"
BASE COUNT         2659 a         6070 c         5820 g         2536 t
ORIGIN      
        1 tcgagaccgg tgtaggcata ggcgcgaatc aggtcgcgcc ccttggtggc gtgcttcagc
       61 agggcgacgt agtcgacgtc gacgtcctgc ccgcgcgcgg cgtagtacat gttggcgacg
      121 tcgacgaaca tcgccacgtt cttgctcacg gtcgagctct tctcctgtgg tatgtgatgt
      181 ggccgacgcg gtggtggcgc tcggcgggcc tcgcatcgag gttgcctccg aggactcgcc
      241 cgcggctcgc gaaccgtgat gccctatggc atcggttcag cggttgcctg gcgcgtgcag
      301 cccttgtctg gtgccgaggc ggcgcggtgg gcgtcggatg ttccgcgcaa cccgcgtcgt
      361 cggcggtcgc cgcggccagc ttggcgctgg cgacccgcgt caggatacca cgcgccgcta
      421 ttgcgcccgc cgagcgagcg ccggtatgat gccgcgcacc tttgatacgc cgggccgcgt
      481 gcgcgcgttt ccccgagcaa tcccattgga ggagagatac ccggatgcag aagtacatgc
      541 gatggagcgc catcctcgtc gtgctgatgc tcgtcctcgc ggcctgccag ggcggcggcg
      601 gatcgagcgc gggcgagagc gccggcgaga gcgagccggc ggccagccag cccgaagcga
      661 gcgagggtgg cggtggtggc accggctcgg ccgccgactg cgaggccgac gagttcggct
      721 gcatcgagct ggccgagggc gatccgctcg tcatcggcac cgcgctggtg atcagcggcg
      781 ccaacgaggc gctcggcacc gacagccagt ggggcgtcga ggtcgccgcg cagttccggc
      841 ccgagatcgc cggccacgag gtcgagttca accaccagga cgacggctgc tcggctgagg
      901 gcggcaccgc cgctgctcgt gccctggtgt ccgaggagaa catcgcggcg gtcatcggca
      961 ccagctgctc cggcgccggc gtcccggctg ccgagatcac cagcgccgag ggcatcctga
     1021 tggtctcgcc ctccaacacc gcgccgtcgc tgaccgcgcc cgagacgcac gagcccttct
     1081 acgcccgcac ggcgcacaac gacaacatcc agggcgccgc catggcgcag ttcgcctgcg
     1141 aggtcctggg cgtcgggtcg gccgccacca tcgacgacgg ctcgccgtac gccgaccagc
     1201 tggcgaacgt cttcgcccgc cagttcgagg agcagtgtga cggcaccgtc acggccgagg
     1261 aggccgtcac ggtcggccag accgacttca gcggtgtgct ggagaacatc gcggccgatt
     1321 cgcccgagct cctgtacttc ccgatcttcg tggccgaggg agcgctgatc acgcagcagg
     1381 cgaaggacac cgcgggcctc gaggacacca tcctcgccgg cgccgacggc tacttcacgc
     1441 ccgactggct ggaggccacc ggtgatgcgg ccgaggggca ctacctctcg ggacctgacc
     1501 tcgccttcag tggcaacttc tacgaggacg agttcctccc ggcgtaccgg gagatctcgg
     1561 gccaggacga gccgatcagc gtgttccacg ctcatgcctt cgacgcctac aacatgatcg
     1621 ccgatgcgat cgaggcggtc gcgttcaccg acggtgggac gacctacatc ccgcgcaccg
     1681 cgctccggga tgcgttcttc tcgacctcgg ggtacgaggg cgtgacgggt acgctcacct
     1741 gcgacgagaa cggcgactgc gctgacgcga agatcagcgt cagccaggtc caggacgtcg
     1801 acggcgagct ccagttcgtc cgcgtctggc cggaaggctg acggttcgca ccagctgaac
     1861 cgatcctgag gatgcgggtg agccgacaac ggctcacccg catcggctaa ccgagaccaa
     1921 accagaacgg gagcaccaga tgcctggccg cccgccccgg ctcaatctcg tcaacctgtt
     1981 cctttggctg ctcgggggcg gcatcctggt gctggccggg gtcggcgcct tcctgacgct
     2041 ctcccagggc cggctctcgg gcgaccagtg ggagtcactg atcgtcgcgg gcatcgcgct
     2101 gggcagcgtc tacgccctca tcgcgctggg ctacacgctg gtctacggca tcctcttcat
     2161 gatcaacttc gcccacggcg aggtgttcat gtggggtgcc ttcaccgcct acttcgcggc
     2221 caccgccctc gacgacgcgg ggatgatgga cgcgaacccg atcctcgcgc tgttcctcac
     2281 cctgctcacc gccatgctcg tgagcatggt catcgccatc atcctggagc gcgtcgcgta
     2341 ccggccgctg cgcggggcgc cgcggctcgt gccgctcatc acggccatcg gcgcgtcgct
     2401 gttcctgcag tacaccgccc gcggcttcta cggatcgggc gtgcgggcgt accccagcta
     2461 cggtgggctc ttcgagggca ccatcgacat cggacccctg accatgccga tcgaggacgc
     2521 cgtcgtcgtg gtcgcagcgc tggccctcat gctggcgctg tacttcttca tcaacaacac
     2581 gcgcaccggg cgcgccatgc gcgccgtcag cgaggacaag gacgtcgcgg cgatgatggg
     2641 catcaacgtc gaccgcatca tcgtcacgac cttcgcgatc ggcggcctgc tggcgggcgc
     2701 ggccggcatc ctctacacgc tcctgttcaa ccaggtgcac ttcttcatgg gcttcctgcc
     2761 cggcatcaag gccttcaccg ccgccgtcct cggcggcatc ggcaacatca tcggcgcggc
     2821 actcggcgga ctgacgctcg gcatcctgga gcagctcggc cccaacctgt tcctgaccgg
     2881 ctacaacatc ccggcgccga accagctcaa ggacgtgctc gccttcaccg tgctcgtcct
     2941 cgtcctcatc ttccggccct ccggcttcct cggccgaaag gagaacgtgt gatgccgccc
     3001 ctcctccgac gcgcggtgat caccggcttc ctcggcggcg ccgccggcat ccacgtcagc
     3061 ctcgtcggca tggtcgagga cttccagggc cgcgacacca ttgccgacat cgtgagcgtc
     3121 ggcaccatgc tgccgatcct catcgccatc gtcgtcggat ggagcgcggg ctcccggatg
     3181 cgcgcgccgc tcatcgaccc cacgccgcgt gagtcgctcc tgttcggcac gctggccggc
     3241 gcggtgaccg gcgcactgat ggcgctgttc gcgctgttca tcaacaccgt cgacgtggcg
     3301 tggatcctct cgaacgccaa gccctccctc gccgatctgc tctggctgga ccagggcccg
     3361 gtggtcggct cgctgctgct gatcatcggt ggtgcgctct tcggcgcggt cggcgctgtg
     3421 ctgcacgtcc tccccggcct gatctcgcgc tcgctggcca tcggcggcgc cgtgaccatc
     3481 gtggtggcgc tgatggagcc gttcctgggc gcggtcctgc gcaacgtcgg gctgcgattc
     3541 ctcgagggct tcctctacgc cagcggcggc ctcacgctgc cgggcttcat cctcgtcttc
     3601 gcgctcgtgg cgggcggcgt ctacctgtgg cgcacgcgcg gacacgggac caaggagcgg
     3661 gtggaggcca tgccggcgga ccgccgtcgc atcggcaaga tcgtgctgta cctggccttc
     3721 atcggcctgc tgctgctgct cccgcagatc gtggggtccc ggctgtcgga ggtggtcggc
     3781 accatcgggc tgtacgtgct cctgggcctc ggcctcaaca tcgtggtcgg gttcgccgga
     3841 ctgctcgatc tcggctacgt ggcgttctac gcggtcgggg cctacaccac ggccgtcctg
     3901 acctccccgg cctcaccgct catcagcccc gagctgccct tctggggcgc gctcccattc
     3961 gtgatcgtgg ccgcggcgat gatcggcctg atggtcggga cgccggtcct ccgactgcgc
     4021 ggcgattacc tggcgatcgt cacgctgggc ttcggcgaga tcgcgcggct catcttcctg
     4081 tcggagtggg ccaagccgat cgtgggcggg gcgcagggca tcctctcgat cccgccgccc
     4141 ctgccgttca ccaaccaccc gcaggtcatc tactacccga tcctcttctt ctgcatcctc
     4201 gccgccatcg cggcatcgcg gctcgcggcc tcgcgcgtcg ggcgcgcgtg gaacgccatg
     4261 cgcgaggacg agtcggtggc ggaggccacc ggcgtgaaca ccacgaagta caagctgctg
     4321 gcgttcgggc tcggcgcggc gttcggctgc ctgtcgggcg ccttcttcgc ggccaagatc
     4381 ggcaccatct tcccgaacag cttcgagatc ctggtcagca tcaatgccct ggcgctgatc
     4441 atcctgggcg gcatgggcaa catctacggc gtggtcgtcg gcgcgttcgt gctggtcggt
     4501 ctgccggagc tgctgcgcga gttcgccgag taccggctgc tgatctacgg cgtggtgctg
     4561 gtggtcatga tgctgctgcg gccggagggc ctgctcccca gccgcagccg tcgcgcggag
     4621 ctgcacgagg accagggtga cgacgaggcg atgtacgagc acgaggccgg cgtcgacacc
     4681 gggcgaccgg tggtgacggg atgaccgaga tgaccgagac cacggcccat tcgacgccct
     4741 cgacagccac cgcccccgag gcgggcgacc tgctccgcgc ggagcaggtg accatgcgct
     4801 tcggcggcct gatcgccaac aaggacgtcg atttcaccat tccgcgcggc tcgatcgtct
     4861 cgctgatcgg gcccaacggg gcggggaaga cgaccttctt caaccagctc accgggatgc
     4921 tgcaaccgac gtcgggcgcc atcacgttcg acggcacctc catcatcggc ctgtcacccc
     4981 acgagatcgt ggagctcggc gtcggccgga cgttccagaa catccggctc ttcggggaca
     5041 tgacggtcct ggccaacgtg atggtcggcc ggcacgttcg catgaagtcg gcgtggtggc
     5101 agtcgctcct cggcctccca tccgtccgtg ccgaggagga ggcggtccga cagcgcgcgc
     5161 gagagctgct cgacatggtc gggctgcgcc gggggtacga ggaggagttc gccaagaacc
     5221 tgccgtacgg cgaccagcgc cggctggagc tggcccgggc cctcgccagc gacccgaagc
     5281 tgctgctgct ggacgagccg acggccggca tgaaccccgg cgagacggcg cgcatgaccc
     5341 agctcatccg ccggctgcgg gacgagctcc agctcacggt cctcctgatc gagcacgaca
     5401 tgaaggtggt gatgggcatc agcgagcgga tcacggtcct ggaccacggc gagctgctgg
     5461 ccgagggaca tccggacgag atccggtcga acccgcaggt catcgaggcc tacctgggca
     5521 agggagccgc gggcacatga gcgaccaggc tcggaccgat ccggtcgcca cggcgaccgc
     5581 ctccaccgct cccgccgccg atgccagcgg caccgcgccg ctcctcgagc tcgacgacct
     5641 ccacgtcttc tacggcaaca tcgaggcgct caagggcgtg tcgatcagcg tcggtcgtgg
     5701 cgaggtcgtg accctcatcg gctccaacgg cgccggcaag agcacgaccc tgcgcgccat
     5761 cagcggcacc cataagccgc gccagggcag catccgcctc ggcggacgcc ccatccacca
     5821 gctcccggcc tacgaggtgg tgaagctggg catcgcccac tcccccgagg gccgcaaggt
     5881 cttcagccag atgacggtct tcgagaacct gcagatgggg gcgttcagcc ggcgcggcga
     5941 gaagatcgat gacgacgtgg cgcgcgtctt cgacctcttc ccgcggctga aggagcggcg
     6001 tggccagaag gccgggacca tgtcgggcgg cgagcagcag atgctggcca tggggcgggc
     6061 cctcatggcg cgcccgtcga tcctgctgct cgacgaaccc tcgatggggc tctcgccgat
     6121 cctgaccgag cagatcttcc ggatcatcac cgagatcaac gagcagggga ccacggtgct
     6181 cctggtcgag cagaacgccc tcatggcgct caacgtctcg tcgcgcggtt acgtgatcca
     6241 gaccggcaag atcatcctgg ccgatacggc cgccaacctc gccgccaacg agcaggtccg
     6301 ccaggcctac ctcggcgaga tctgaccctt ggcgtcccgt ggctgaagaa ccgcagccgc
     6361 cgccgtcccg cccgaagggc ccgggcgact accgcccgga cctgcgcccg atgctgatcc
     6421 tcggcgtgct gctggtggcc gtggtcctcg cctggatcct gctgtcgccg ctgatcctgc
     6481 cctcgaccta gtagcgccgc ggcgccccga agccggcacc cgcgccgtag agcaggagcg
     6541 ccacccccac ggccgccagc gccagccccg gcgactgctg gggaagcggc agataccccg
     6601 ccgaccacag ctcccagccg cggtacgcca gcgccgcgcc gccgaccagc ccgaggacca
     6661 ggtacgtcca gggggagtcc aggaagaaac cccaccgccc gcgcatgtag ccgacgtcca
     6721 gcagcacgag cgccaggatg gcgacgccgt agatcacgag cccgggtccc tcgagcccga
     6781 tcccctgcgc cgcgggcacc tgcaccccgg acaccgactc gcccccggac cgccaccacg
     6841 gcacgaagcc cgatccggtc atggccaggc tgccgagcag gaccaggagg cggaagcggc
     6901 gcccaccccc gtccacggat cagccctcgg gcggtgcacc gccgtagacc gacggcttga
     6961 gcacgccgat gtacggcagg ttgcggtagc gctcgtcgta gtcaaggccg tagccgatga
     7021 cgaatcggtc cggaatcgtg aagccgcgat aggcgatcgg gatctcggcc aggcgacgcg
     7081 ccggcttgtc cagcaggcag acgatctcca cggatgcggg ctgcttgtcc gcgaggtact
     7141 tcagcaggta gttgagggtc agcccggtat cgatgatgtc ctccaccacg atcacgtggc
     7201 gcccctcgat cggtcccgac aggtccttca ggatgcgcac ctggccgctg gtctcggtcc
     7261 cggagccgta ggaggagacc tccatcaggt cgacgctgac cgggatctcc atggcgcgca
     7321 tcaggtcggc caggaagatg agcgatccct tcagcacgct gaccagcacc ggatcgcggt
     7381 cggcgtagtc ggtcgccagc tgcgccccca gctcgctcac ccgggccgcg accgtcgcgc
     7441 ggtcgagcag gatctcctcg acgtcgtcgt gcatggtcat cgcgccgcca ccgccaccgc
     7501 accgttggcg gcagcgttgc cattgccggt cccgttcggg ccgtgggtgc tcccgttggc
     7561 cgccgtcaca tcggtcagga agtccaggcg gccgtacttg agcatcaggg ccttgaagtc
     7621 acgggcgtag aacagcttca cgcgtacctc ggggtagagc tgccgcatcc agcgcagctt
     7681 gcggttcttc ttgcggacga gcgactgctt cagcgtcgtc agctcgacgt acaggtcgat
     7741 ctcgggcagg tagaagtcgg gcgcgaacga ctcggtgacc gcgccgtcgg cgttccagga
     7801 gatggggaag acgtcgggtt cgtatcgcca cgcgacaccg tagaagtcga ggatcctcgc
     7861 cagctccgcc tcgctctcgt gggcgaaacg gggtggcgag tccgaggtca tccgaaccgc
     7921 cacctgacct cgatcgtacc gtgcccccgg acctgcgacg ccatcgccgt cgagtatatc
     7981 cgccggagtt atccacattc gggccgggcg tgccaaacgt gtcgataact tccgttgtga
     8041 tccacaccgg gccggtgcta cggtgtccgt cctgcttctg cgggcgaccg agtacggtaa
     8101 ccgtggacgg tcggggccga tgcgaaagcc ggccgcgacg ccgaccatcc gcgggaagac
     8161 cgaaacgtcg ctcgacagga gcatatttct tgtccgccac gccctcccgg accgtcgcgc
     8221 gcgccgcgcg cgacgcgctc gagcagctgc tcgcgaccga tgctcccgcg ctgcgccggc
     8281 agctgccccc cgacttcctc gatcgcgccg agcgctacgt cgcgctgctc ctcgagacga
     8341 acgccaggat gaacctgacc cgggtcgtcg atcccgacgc cgtggcccga ctccacctgc
     8401 tcgacgcgct agcggcgctc gggctgatcg acgccgtcgc gccgtcgctc gcgctcgacc
     8461 tcgggtccgg cggcggcgtc ccgggcctgg tcctggccat cgcgcgtccg aatattcgct
     8521 gggtcctggt cgactcggtc cgcaagaaga tcgatgcgct gcgcaccttc gccgctgcgc
     8581 tgggcctccg gaacgtcgag gtgctggcgg agcgcgcgga gatcctcggc cgcgaccctg
     8641 aacgccgcga ccgtgcggac ctcgtgaccg cccgggcctg cgcatcgctc cccgtcctgc
     8701 tggagtacgc gctgccgctc ctgcgggtcg acggccggct cgtcgcgtgg aaggggccga
     8761 tcggcgacga cgagctctcg gcgggcgggc gggcggcggg cgagctcggg ggcggcgtgc
     8821 cgtgggtcga gccgaccggc gtcgaggcgc tcggggaaca tcggttcgtc ctggtcgaga
     8881 aggtgcgtcc gacgccggcg cggttcccac gccgaccggg tgaggcggcg cgccggccgc
     8941 tcggcgggta ggcggggtcc gctgccgcga gcgccgtgca ctgtgatcaa ttccacgcat
     9001 cggcctgatg agaagcgcgc gctatcctga cgcctccggt gatggggctc gccgtgaacc
     9061 gcccgctcgg gctgatggct cctaccgaac cgtcgctcga tcgctcacgg agaccgccat
     9121 gctctcgctc ctgctcgtcg gcctcggagg cttcgccggc gccgtggccc ggtacatcgt
     9181 ggacgcccgc gtctcggcgt ggaccggcgg cacgctgccg tgggggaccc tcgccgtcaa
     9241 cgtgaccggc tccttcctgc tcggtctgct cttcgcgctc gtcatcgagc gggcggcact
     9301 ccccgccggc ctgcgcgcgc cgctgatgat cggcttcatc ggctcgtaca ccaccttctc
     9361 gacgctcgcg ctcgagagct ggcgcatgat cgaggacggc gcatggttga ccgccggcgc
     9421 caacctggtc ggatccatcc tcatcggcgt cgtcgccgtg gtcgccggcg tcgcgatcgg
     9481 gaggtcgatg tgacgaagct cgagggcacg ggcctgctgg tccgcatcta cctgggtgag
     9541 agcgatcggt ggcacggcag gccgctgtac caggcgatcg tcgagcggct ccgtgagcgc
     9601 ggcctggccg gcgccaccgt gctgcgaggg atcgaggggt tcggggcgaa gcagcggctg
     9661 cactccacgc gcatcctctc cctctcggag gacctgccga tcctcatcga ggccgtcgac
     9721 accgaggaga agatccgcgc cacgctgccg gagctcgacg agatggtgag cgacgggctc
     9781 atcaccatcg agcgcgtcga ggtgatcacc taccgcgcgt cgccatccac ctgaggaccc
     9841 gccgtatata ccgatatacg cagtcgtcga cgaggggcgc ctcggtatac tccacgctcg
     9901 catgcgcatc gccctcctct cggacatcca cgccaacctg gcggccctcg aggcggtggt
     9961 cgccgatctt cccgccgtgg acgaggtctg ggtgctcggc gacaccgtgg gctacggccc
    10021 ccagccgaac gaggtgatcg ccatgctgca ggccatgggc gcgcgcagcg tcctgggcaa
    10081 ccacgacggg gcggccatcg gctgcgtcga cgccgatccg ttcaacccgg acgcgcgggc
    10141 ggccatcgac tggaccacca ccgtcctcga cgagaacagc cgcgcctaca tcgcgaccct
    10201 ccccgaggtc cgcgtcgacc gtgccatgac ggcggtccac ggctccccgc gcgacccgat
    10261 ctgggagtac atcaccagcg cctcgatcgc ggcggcgaac ttctccgcct tctcgtcgcg
    10321 actctgcctg ttcggccaca cgcacctgcc ggcggcctac cgcgccgtcg acggtcgggt
    10381 cgaaggcgtc cccggcatgc ccggcgacgt cgtccgcctc cacggcgcca gggccatgct
    10441 gaatcccggg agcgtcggcc agccgcgcga cggcctgccg gcggcggcgt acgccatcct
    10501 ggagacgaac ggggacgacg aggccgatgc gatcgagttc cggcgggtcc gctacgacgt
    10561 cgaccgcacg caacgcatca tgcgcgaggt cggcctgccg ccgcgcctcg ccgaacggct
    10621 gagctacggt cgctgagcgc ggcacgccgc gccgatgcgc cgcccgggcc atgggcgccg
    10681 taggatagcg ctcggcatgg acttctttcg acgctccgag aagcgcccgt cgcacttccc
    10741 ctaccgccgc ctgctggtgc cgctgctcgg cgacccagac gtcgacgacc ccgcgctgcg
    10801 gctggccgtc atcctgctca gcggccgcga ggaggcggag gtcgccctgc tccacgtgat
    10861 cgaggtgccg ttcgagcgca acctcgacgc ggaggaccag cgagccatcg aggcggcgga
    10921 cgagatcctg gcacgcggcg agcatctgct gacggagcac gagatcagaa cgcgcacgag
    10981 caccgtccag gcgcgggccg cgggaccggc catcgtcgat gacgccaacg agctcggcgc
    11041 ggagctgatc gtgatgggac ttcgctacaa gaagcggttc ggaggacaat gggacgcggg
    11101 acgcacggtc ccgtacgtca tgcgcaactc gaccgcgccg gtctggtgcc tgcgcgccga
    11161 gacgaaggag ctggcacaca cgccatgagg gtcatcatcg tcggctgcgg ccgggtcgga
    11221 gcccgcgtcg ccgcggagct cgacgagcgg ggcgagaacg tcaccgtcat cgacgtcatc
    11281 cagcgtgcct tcaaccgcct gccgcagggc ttcggcggcg agacggtgcg cggcaacggg
    11341 acggacgagg acatcatgcg ctcggccggg gccgagcagg ccgacatcgt catggccctc
    11401 accgagggcg acaaccgcaa cgcgctggcg gcccagctcg cgaagcacac gttcggcgtg
    11461 ccgcgcgtca tcgtgaagat caacgatccc gttcgggccg aggcataccg gacgctgggg
    11521 ctcgagacca tctgccgcac gctcatgctg ggcgacgcgc tggtcaccgc cgccatggag
    11581 ggcgccgagg cgaccaacgg gttcgtggag gcgccgaccg ccgagcccct gcgcggggct
    11641 ccggtcccgg gctcgctggc ggacgccgag gctcgggccg ccatggcggc cgatgtcgac
    11701 gccacggcgc ggtccgtgga gcccaccgcc gacgagccgt ccggcaaggt cctgccgcag
    11761 ggcggtgtcg agggaggcat gtagatgtac gtcgtggtga tcggcggcgg caacgtcggg
    11821 tactacctga ccaaggagct cctggcggcc ggccacgagg tggtggtcat cgaggaggac
    11881 cagcaccggg cgcgtcagat cgcggacgag ctggggagca tcgtcatccc gaacgacggt
    11941 tgcgagggcc gataccaggc cgaggccggc atggcccgcg ccgacgtgat ggccgccgtc
    12001 acgggtgacg acgcggtgaa cctggcggcg tgccagatcg caaagatgcg cttcaacgtg
    12061 ccgcgctcga tcgcgcgcat caacgatccg aagaacgaga agctgttccg gcagctggcc
    12121 atcgacgagg tcgtcagccc gacgcgctcg atcctcgggg tgatcgagca cgagatcccg
    12181 atccacgacc tcctgcacct cgcggagctg gagggtggcg aggtccagat cgtggaggcg
    12241 cagctggacg gtgactcgcc ggccatcggc cgcgagctgc gcgagatcga gctcccggag
    12301 ggcagcaaca tcgccgtcct catccgcgac gaccgcgcca tgccggcacg ctcggacgtc
    12361 cgtctgcggg ccggtgacaa ggtcctggcc gtgacctccg ccgaggacga ggccgagctg
    12421 cgcagcctgc tcatcggcga gtgaccggcc ccgatccggg gcgcgtcctt cgcctggcgc
    12481 tcctcgcctg gggcctcgga cacctcgcca tcggccggcg cgcgctgggc cgcgggctgc
    12541 tgatcgccga gatcgccggt atcgggctgg tggcctggct gaccgcgagc ctggccgact
    12601 ccagcgccta cctgctcccc ttcctggccg gcattacctt cctggcggtc tgggcggcac
    12661 aggccatcgc cgcctaccgc gaagcccatg cgctgcaggc cgctcgagcg ccgacgcccg
    12721 cgcgctcgcc ggccatcgcc atcggctggc tcagcctgcc gctgctggcc tgggcgaccg
    12781 gcttctggct catcggcgct cgcgacgcga acccctcggc cgtgctcgac cgcttcgtca
    12841 ccgcctggag caccgacggc ctcgacggcg caggctggcc acgagcggtc gtcggcgctg
    12901 cgaaccgcgc ggcgcgggat ctcgggaccg atgccgaccg gttccgcgac gttcgcgtcc
    12961 ggatcgtcga gcaggacgac cggcgcgcga ccgccgtggc ggaggcgatc cacttcgagc
    13021 gccgcgcgac cacgttgctc ggcctcttcc cgggcacgga gctggtcccg gtcgcggacg
    13081 agctggtgct ggagctcgag ctgcgcgcct cgcccgtcga gctggccggt ggcggcgaca
    13141 tcggcgccgt gcactgggag ctcgtcgccg ccgacgctcc ctgacgaacc gcccccgcac
    13201 ctccgggtgc cgcccagggc gacgcatcgg tcctgtcaca agggttgaca tcaccattgt
    13261 cagaccttat ggtttcgaca cgcaccgttt cacccgtgcg tttcgacaga cgaccgtgtg
    13321 ggatatcgcg tcaaggaggc aacagctcat ggcaaacatc acgcgttacg acccgcttgg
    13381 agagatggtc tcgctgcgac aggcgatgga ccgcctcttc gaggactcct tcgtcagccc
    13441 gctgagctgg cgcacgatct cgggcgggga gagcatcacg cccgccatcg acgtgcacga
    13501 gaccgcggat gacgtcgtca tcacggccgc cctgccgggt gtcaagccgg aggacgtcga
    13561 cattaccatg accggccaga gcctgaccct gaagggcgag ttcaaggccg acgagagcat
    13621 cgaccgcgac cagtacctct accgggagcg ccgatacggc accttcaccc gcagcctgca
    13681 gctgccggtt cgcgtcaagg gcgagggcgc cgaggcgacc ttcgccgatg gcatcctcac
    13741 ccttcgggtc ccgaaggccg aagaggtcaa gccgcgacag atccggatca atgccagcgg
    13801 caacggcggg cagacgcagc cgtaggatgg accgatcgcg gcgcccggcg ccacaccacg
    13861 gcgtcccgcg ccgcgacgat ctgacccggc cgcgctacat catcagcatc gccgcggagc
    13921 tggcgagctg tcatccacgg acgctgcgga tctacgagga ggagggcctc ctcgccccgc
    13981 agcgtcggaa caacctccgt ctctacagtg aggcggacat ccaacgcgtt cgtatcattc
    14041 gattcctcac acgccgccag cgagtgaacc tcgcgggtgt gcgggtcatc ctccagctcg
    14101 aggatctcgg gaagatccgg gtctacgacc tgttcgatga ggacgatatc gagcgattcg
    14161 ccgagaacga cccagatcaa ccggccgagg cctcggccga gggagcgacc tgagtggcta
    14221 ccgaagacac ccgagagaac aacgagatgg aggcgaccgc cgaggacgcc acggccgcgg
    14281 agcgcatcca ggtcgtgccc ctggtggcgc tccgcgacac cgtcatcttc cccgagatga
    14341 tcgtcccgct gcaggtcggc cgtgaccgca gcgtcaaggc gctcgaccgc gccgtccgaa
    14401 cctcacagcc ggtcgcgttg gtgacccagc gggtcaacga ccaggaggag atcaacgccg
    14461 ccgaggagct gtatgccgtc ggcacgctgg ccaagatcgc ccaggtcatc cggctgcagg
    14521 acggcaccgt ccgcgccatc gtgcagggcc agaagcgcat ccgcctgctg gatctgctcc
    14581 agaccgatcc gtacctcgag gcgcgcatcg aggcggtcga cgacagctcc gagaagacgc
    14641 tggagatcga ggccctgatg gcgtcgatcc acagccagat cgagcagtac gtcaacagcg
    14701 gcgcatcggt cccgccggag gtggcggtcg cggcgcgcaa catcaccgac ggcggcctgc
    14761 tggccgacat gacggcctac agccccgaga tgaccacgga gcagcgccag gagctgctcg
    14821 agaccatcga cgtgccggat cggctgcgcc tggcgagctc gttcctggcc aagcagatcg
    14881 aggtgctcga gctcaagggt cggatccaga gcgaggtcaa gtccgagatg gacaagaccc
    14941 agcgcgagta catcctgcgc gagcagatga aggcgatcca gaaggagctt ggcgaggatg
    15001 atccggccgt ggccgaggcc aacgagctcc gcgagcgagt cgagcagtcg gccatgcccg
    15061 aggaggtcaa ggagaaggcc ctcaaggagg tcgatcgcct gagccggatc ccgagcgcca
    15121 gcccggagca gggcgtcatc cgcacctacg tcgactggct ggtgagcctg ccatggggcg
    15181 tcgagaccga tgacaacctg gacctcgagg aggtcgagcg ggtcctgaac gaggaccact
    15241 ggggcctcga gaagatcaag gagcggatca tcgagtacat ggccgtgcgc aagctggccg
    15301 agaagatccg cagccccatc ctcctcttcg tcggcccgcc cggcgtgggc aagaccagcc
    15361 tgggcaagtc gatcgcgcgc gccatgggac gcaagttcgt ccgcatgagc ctgggcggca
    15421 tccacgacga ggcggagatc cgcggccatc ggcgcacgta catcggcgcg ctgcccggcc
    15481 gggtgatcca gaacatcaag accgccggtt cggcgaaccc cgtgttcatg ctcgacgaga
    15541 tcgacaaggt cggcatggac ttccgcggcg acccgtcgtc ggcgcttctc gaggtgctcg
    15601 acccggagca gaacttcagc ttccaggaca actacctgga ggtgcccttc gacctgtcga
    15661 aggtgctgtt catcgccacg gccaacatgc tcgacaccat cccgccggcc ctgcgcgacc
    15721 ggatggaggt catccagctg cccggctaca cccagctgga gcgggtccgc atcgcggagc
    15781 gcttcctggt gccgaagcag ctcgaggcgc acggcctcaa ggacgaccag ctcgtcttcg
    15841 aggagccaac cctggtccgg ctgatccagg ccttcacgcg tgaggccggc gtccgcaacc
    15901 tggagcgcga gattgccaac gtggcgcgca aggtcgcccg tcgcgtcgcg accgacgccg
    15961 atgcgaaggt caccatccac cccgaggacc tcgaggagta cctcggcccg gctcgcttcg
    16021 agtacggcga gctcgaggcg gaggaccaga tcgggatggt gacggggctc gtcgtcagcg
    16081 acgcgggcgg cgacatcgtc caggtcgagg cgacgcgcat ggagggcaag gacgagttca
    16141 tcctcaccgg ccagctcggc agcgtgatgc aggagtcggc acgggccggg atgagctaca
    16201 tccgggcgcg cacgaaggag ctcgggatcg acccgaccgc cttcgagaag acgaccgtcc
    16261 acatccacgt gccggccggc gcgacgccca aggacgggcc gagcgcgggc gtgaccatgg
    16321 ccacggccat ggcgagcctc ttcaccggca agccggtccg tcgcgatctg gccatgaccg
    16381 gcgagatcac gctccgtggc cgcgtcctcc cgatcggtgg cctgaagcag aagctgctgg
    16441 ccgcccacct ggcgggcgtc aagacggtgc tgatcccgat gcggaacgag aaggacctgg
    16501 tcgacgtgcc cgacgaggtc cgcgagcagc tgcggatcgt gccggtcgag aacatggacc
    16561 aggtcttggg cgaggcgctc atcgacgcgc cgcggtcggc ggcccggatc aaggcggagc
    16621 gcgacgagcg gcagaagcgc gccgcgcgac gtcctcgccg gtcccgcaag cccgccaccg
    16681 aggagcctcc catcgccgcg accggcgacg tggagcagcc gcagccaccg gcggggcagc
    16741 cggcctgagg gcgctcatgc gcgactcgat gtatcgagac cgatccgcag gaccggcccg
    16801 ctccgctgcg gatcctcggt acatcgagtc gtgggctgca cgagcggcgg acggttggcc
    16861 cgcccgcgat gccaccgtca cgatggatcg cgagaacggc gccacacacg ccggtcgcgt
    16921 gatcgtgtct cgattcatcg aggcctgacc gatggactac aaggactact acgcgatcct
    16981 gggcgtcccg aagacggcga cgcaggccga gatcaagaag gcctatcgca agctggcccg
    17041 tgagctccac ccggacacga acaaggaccc ggacgcggag aagcg
//
LOCUS       DASTYP010000204         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_528570, whole genome shotgun sequence.
ACCESSION   DASTYP010000204 DASTYP010000000
VERSION     DASTYP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528570"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFV59_09030"
     CDS             complement(<1..749)
                     /locus_tag="VFV59_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326009.1"
                     /translation="MTRTTRKGLLARIRRPVRREGAEGYLFVTLVSFATTVIATRWFL
                     SLTGFPRIGGGDLHIAHALWGGVALFVAALLPILLAGRFVYFASAALSGIGIGLFIDE
                     IGKLITADNDYFYPAAAPIIYATFLLAVLVWLRVRGARQVEEDPRTQLLTALELLEES
                     VEGDLQQSERETLRERLTGAASRAQGGEQRRLAAELLAFVEAEETRISPDPVDRLLAV
                     RRWWSAREARWLGGRGLRLAIVAALVVTGVV"
     gene            861..1598
                     /locus_tag="VFV59_09035"
     CDS             861..1598
                     /locus_tag="VFV59_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEU5326010.1"
                     /translation="MRPRAHRTSAVVLSALFLTALPLPASAARPPKPPSGPSTISWSG
                     HDWDVKEYSRKIGPGPNFFSSSNVRVDPDGNLHLAITKSGSKWTTAEVILKDSFGYGT
                     YTWVLRTPAPLDPNVVLGLFTWNDDPAENHREIDIEFAKWGNASDPNDAQFVVQPYDS
                     VGNEHRFVRPAGGALTTLSFTWASDGVTFRASSDGVAQTWTYGGADIPLPGGENPRMN
                     LWLFRGAAPTDGRSVEVVFESFTFTRP"
     gene            complement(1818..2282)
                     /locus_tag="VFV59_09040"
     CDS             complement(1818..2282)
                     /locus_tag="VFV59_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326011.1"
                     /translation="MTDDAAALSRDLPGEEEAWERLHHVIDQVTPETAEVPGYFEEGW
                     TAKDAIAHLGTWMARGATVLRQIAAGTYQEGEIDVDAENARYLDAMRDIPLETVHLQA
                     AAAHGELLRAWRELPEVTPAAAYWVRKAGPEHYAEHLPRLESWVEQLSTPGR"
BASE COUNT          375 a          925 c          890 g          354 t
ORIGIN      
        1 tgcacgacgc cggtcacgac cagcgcggcg acgatcgcca gccgcaggcc gcggccgccc
       61 aaccaccgcg cctcgcgcgc ggaccaccag cgacgcaccg cgagcaggcg atccaccgga
      121 tcgggcgaga tccgcgtctc ctccgcctcg acgaacgcca gcagctcggc ggcgagccgg
      181 cgctgctcgc caccctgcgc gcggctggcc gctccggtca gccgttcccg aagcgtctcg
      241 cgctccgact gctggaggtc gccctcgacc gactcctcga gcagctccag ggcagtgagc
      301 agctgcgtgc gcgggtcctc ctcgacctgg cgcgcgcccc gaacccgcag ccagaccagc
      361 acggcgagga ggaaggtcgc gtagatgatc ggcgccgcgg ccgggtagaa gtagtcgttg
      421 tcggccgtga tgagctttcc gatctcgtcg atgaacaggc cgatgccgat gccggacagc
      481 gcggccgacg cgaagtagac gaaccggccc gccagcagga tcggcagcag cgcggcgacg
      541 aagagggcga cgccgcccca tagggcgtgc gcgatgtgca ggtcgccacc cccgatgcgc
      601 gggaaaccgg tcagcgacag gaaccaccgc gtcgcgatga cggtggtcgc gaaggacacc
      661 agcgtgacga acaggtagcc ctcggcgccc tcccggcgca cgggtcgccg gatgcgggcg
      721 agcaggcctt ttcgggtcgt gcgggtcacg gcggcgacgg tagcagttgc cctcgcgcaa
      781 cgggagctgg gcccccggtc tggacagcgg tgggtcccgt cctgcagcct atcggccgcc
      841 cgtacgaagg tactgcaccg atgcgacccc gagcccaccg caccagcgcc gtcgtcctca
      901 gcgcgctgtt cctcaccgcg ctccccctcc ccgcgtccgc ggcgcggcct ccgaagcccc
      961 cttccgggcc gtcgaccatc tcgtggtcgg gtcacgactg ggacgtgaag gagtacagcc
     1021 gcaagatcgg cccggggccg aacttcttct cctcatccaa cgtccgcgtc gatcccgacg
     1081 gcaatctcca cctggcgatc acgaagtccg gcagcaagtg gacgaccgcc gaggtgattc
     1141 tcaaggacag cttcggctac ggcacgtaca cctgggtgct gcgcacaccc gcgccgctcg
     1201 acccgaacgt ggtcctgggg ctcttcacct ggaacgacga ccccgcggag aaccaccggg
     1261 agatcgacat cgagttcgcg aagtggggca acgcgtcgga cccgaacgac gcgcagttcg
     1321 tcgtccagcc gtacgacagc gtgggcaacg agcatcggtt cgtccgtccg gccggcggtg
     1381 cgctcacgac cctgtcgttc acctgggcgt cggacggggt gacgttccgc gccagctccg
     1441 acggcgttgc ccaaacgtgg acgtacggag gcgccgacat cccgctcccc ggcggcgaga
     1501 acccgcgcat gaacctgtgg ctcttccggg gcgccgcgcc gacggacggg aggagcgtcg
     1561 aggtcgtgtt cgagtcgttc acgttcaccc ggccctgatg ctcgcgactc ggccagctac
     1621 gccctggagg gcgtggccgg tcgcgtcgtg gcgttcgacc gtgcgagccc acgccgggga
     1681 ggcgtgaaac gggttctttt ggcaggctgg cgacgatcac cggtgccagc cacgcctcgg
     1741 gggcgtacct ggcaggcttg gccacgggac ggggctgcgg cgatggcgcc ccgtccagca
     1801 gaccggcgcg gatcggctca gcggccgggg gtgctcagct gctcgaccca ggactcgagg
     1861 cgcggaagat gctcggcgta gtgctccggg ccggccttgc gcacccagta cgcggccgcc
     1921 ggggtcacct ccggtaactc gcgccacgcg cggagcagct cgccgtgggc ggccgcggcc
     1981 tggaggtgaa cggtctcgag cgggatgtcg cgcatggcat ccaggtagcg cgcgttctcc
     2041 gcgtcgacgt cgatttcgcc ctcctggtac gtcccggccg cgatctgccg cagcacggtg
     2101 gccccccgcg ccatccacgt cccgaggtgg gcgatggcgt ccttggcggt ccagccctcc
     2161 tcgaagtagc ccggcacctc ggccgtctcg ggcgtgacct ggtcgatcac gtggtgaagg
     2221 cgctcccacg cctcctcctc ccccggcagg tcgcggctga gcgcggcggc gtcatcggtc
     2281 atggcggtga tgctacgacc tcgacggccg ggtgcgaacc cctgttcagc ggcccgttgg
     2341 acgtgcacga tgaatgtttc gaacccctgg tgcgtcggta tcggcatgac gggcagaagc
     2401 ccggcacggg gaggcggagc tgagcgagga agcccgagcc atcgcgtccg atgccgtggc
     2461 ggcacgaacg gccgccttcg agcggctggt cgccgagcgc ctcgacggca gctaccgcct
     2521 tgcccgcctc atcctccgcg acgc
//
LOCUS       DASTYP010000205        21025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_91027, whole genome shotgun sequence.
ACCESSION   DASTYP010000205 DASTYP010000000
VERSION     DASTYP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 21025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21025
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91027"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VFV59_09045"
     CDS             complement(<1..915)
                     /locus_tag="VFV59_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326012.1"
                     /translation="MKHEIRYGKADVKVYRTYAEPLTGVTPIPESPFAGRDNTLAAAE
                     IEVVVHGTEFLDAYTTGDNRKVVATDTMKNFIHATSLRARARTLEEWLLEVGTAFLDT
                     YPHMERLTMHGRELPFPAAVVPADDRDGFAASDRLFSRDRCDRGTARLELARDADGAV
                     RITDHESGREGLQLIKITGSAFADFARDEHTTLPERRDRPLYIWLDISWRYTDASHAV
                     LAEPARYVAAEQVADLAAAVVPADDRDGFAASDRLFSRDRRDRGTARLELARDADGAV
                     RITDHESGREGLQLIKITGSAFADFARDE"
     gene            complement(976..2013)
                     /locus_tag="VFV59_09050"
     CDS             complement(976..2013)
                     /locus_tag="VFV59_09050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5326013.1"
                     /translation="MPLDVRDFAPEDRAWAAALLGAHGGGVPHVARSGELLDPLEHEG
                     IVAELDGRPVALLTVSESEKGLEVLSLHSEVRGVGAGTRLLETALRVAVASEAPRLWL
                     VTTNDNVDAIRWYLRRGMHVAAVHSGGVDADRSAIKPEIPRENPENGLPVRDYVELEL
                     RTDAGDRLPFAPFSDIADLDALPAEPAADLLRTLFEDAPRFLDRLAAARPFVSDAGLI
                     ACAQELARTVPEDEQVELLNAHPRIGADPAAVSVLSHAEQGYDQPDAPPTPPWVAEEL
                     EALNDAYERVFGFRFVVFVAGRSREEILPLLERALRDEREGELRRGLDDVVDIAADRL
                     STLRGQASMPG"
     gene            complement(2019..2366)
                     /gene="uraH"
                     /locus_tag="VFV59_09055"
     CDS             complement(2019..2366)
                     /gene="uraH"
                     /locus_tag="VFV59_09055"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEU5326014.1"
                     /translation="MPAARPTLSTHVLDLATGDPAEGIAVTLFRLDPSGTPELVSELE
                     TDADGRIRDLLDGAELVAGEYQLAFDVGGYADEEDDFFQSVALALRVVEIDRSYHVPL
                     LLSPFGLTTYRGS"
     gene            2396..2977
                     /locus_tag="VFV59_09060"
     CDS             2396..2977
                     /locus_tag="VFV59_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5326015.1"
                     /translation="MTDAPVGRDARVDLLLTGSLRPRVRSSCTLVREGRVAAVVDPGL
                     APSAAAILDPLRALGLTPGDVTAVVLSHHHPDHTLNAALFPNAEVHDHWAVYRGDDWV
                     TRECEGAQLSPSVRLLRTPGHAAEELSTAIGTPDGVIVATHLWWTADGPADDPYAPDR
                     DQLRRSRERVLAIADRIIPGHGAPFRPGDATPR"
     gene            3000..3413
                     /locus_tag="VFV59_09065"
     CDS             3000..3413
                     /locus_tag="VFV59_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEU5326016.1"
                     /translation="MADLHIRVGDLAFTADWEADAPRTRDALRTWLPIRSKLIHCRWS
                     GESTWIPFGDRRPDVGYENHTSHPAPGQLLIYTGEISECEILFPYGACMFSSKLGQLA
                     GNHFATLVSGQEQLAELGRRVLWEGAQEIEISETA"
     gene            3410..4765
                     /gene="hydA"
                     /locus_tag="VFV59_09070"
     CDS             3410..4765
                     /gene="hydA"
                     /locus_tag="VFV59_09070"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEU5326017.1"
                     /translation="MTDLVVRGGTVVTAEGSVAADVAVTDGRIVAVGRGAGAGAFRVM
                     DAAGLLVLPGAIDVHTHLRLPVPERPDRFRADTLAAARGGTTTVVSFNNPGTGISEEG
                     AASPLAGLREFRERTAGESAVDYALSAVIGSHQPDPLREIPELIASGVPTFKAFMVYD
                     FALPDDQIRELMGLAARHGGMLQLHCEEPAIIEPLIADALARGDTGPRHHAHTRPPHA
                     EAAAVQKAIAWARQAEAPLYIVHLSSAAALEHVADAKGRGEPVYAETCPHYLTATDDV
                     YADPDDREVMKRVISPPLRSQADVEALWAGLRDGVLDVIGSDHVPDRLDTEKRLPAPP
                     FPEISNGGPGVETLLSVVWSEGVAARRISPARLVQLLARNPSRLFGLPSKGAIQVGRD
                     ADLVVWDPEARRTIRQADLHHSSDFTPFEGLEVVGAPVEVLLRGEPVAEARGRFLERR
                     L"
     gene            complement(4835..5155)
                     /locus_tag="VFV59_09075"
     CDS             complement(4835..5155)
                     /locus_tag="VFV59_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEU5326018.1"
                     /translation="MTFQDKTLNCQDCGMDFTFTARDQEFYAERQFSEPRRCPACRAS
                     RKAQRGDSAGGYGGGGGGYGGGYSDRPREMFSATCDSCGKEARVPFRPTGSKPVYCSD
                     CFRR"
     gene            complement(5298..5918)
                     /gene="lepB"
                     /locus_tag="VFV59_09080"
     CDS             complement(5298..5918)
                     /gene="lepB"
                     /locus_tag="VFV59_09080"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5326019.1"
                     /translation="MIRPPEPNPVPEPLVPGWPADIRRWVLEVARTVLFVAALYLVLN
                     TFVVQPVEVEMRSMQPLLVEGDHLLVDRLSLRWDAPDRGEVVVFDAPSPYGDDGVPYV
                     KRVIGLPGETIQIENGRIYVSGPDAPPFRLDEPYLTDGTVTLPQGPAGSREWRVPEGS
                     VFVLGDNRSDSLDSRTFGPIGLDRLIGRALVRYLPLDRIGFVSSRD"
     gene            6006..6428
                     /locus_tag="VFV59_09085"
     CDS             6006..6428
                     /locus_tag="VFV59_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5326020.1"
                     /translation="MARDEKLDHLRRVPLFSRMSSREIERLGQLADEVTVGMDQVLAQ
                     EGRYAEEFFIVLDGRVTVLDGGRPIAELGPGDFFGEIGLLDGHRRTATVRADGITRLL
                     VIGHREFNALMDEFPSVREAVLLAVEDRVARSRGTAAS"
     gene            6510..7679
                     /locus_tag="VFV59_09090"
     CDS             6510..7679
                     /locus_tag="VFV59_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5326021.1"
                     /translation="MAGTPNVLVIGSGSTGSAVAHDLALRGVAVTVVERGEIASGTTG
                     RNHGLLHSGGRYAVKDAESAIECINERDILAAIAPEVLELNGGLFVALDEDDLAYREP
                     FLQACATCGIPTRELSTQEARELEPALSARVLAAIEVPDGVFDPLHLCLAFLATAQGN
                     GARVRAYTEVEELIVRGSSVEGAIVRDRRTGERRRVDADLVVNATGPWADRIAAMAGL
                     DVPVVPTAGVMVAMAGRFVDRVINRLNKPSDGDIVLPQRRTVVIGTSSWQVENPDLVT
                     IPADHVALMAERGGEMVPSVRTAPMRGVFAASRPLIGRPGDTDGGRELSRTFECFDHA
                     REGVSGFVTISGGKTTTARAMAEKTSDVVCEQLGVSAACRTETVPLRSYRDFYRG"
     gene            7672..8409
                     /locus_tag="VFV59_09095"
     CDS             7672..8409
                     /locus_tag="VFV59_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5326022.1"
                     /translation="MAEQTVTARVWRQAGPGARGGFRSYEVGVDPAADSVLDVIERIW
                     AERDHSLVFRHACHHASCGTCAVVVDGRERLPCITPLSEVWDGRSPITLEPLRNVELV
                     ADLAIDPALALQRLAELPLPYVRTVEAAVRAASGVERDPDVGAAERFEDCLECLACVS
                     ACPVAAGDPEYLGPAVLAAAHRAVEEPRNGGTARILALVDTDHGVWQCRSIWACSAVC
                     PSAVDPAQRIMDLRRRLWGRRREAAHG"
     gene            8402..8794
                     /locus_tag="VFV59_09100"
     CDS             8402..8794
                     /locus_tag="VFV59_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326023.1"
                     /translation="MAERGMPVRAHRPFAWLQPRWRDAGWWAFVLNRLTGLILVAYLL
                     AHFMVLSLLTQGARGWNQLLELFGSTPFLVGDVLLISAVAFHGLNGLRVIGLTFGLGA
                     RHPTLTVFVVLGISAVLGALVGWLVLFA"
     gene            8791..9243
                     /locus_tag="VFV59_09105"
     CDS             8791..9243
                     /locus_tag="VFV59_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326024.1"
                     /translation="MSAPIPVRRFPGRPDAAPPGFVPGAERLEPTIEELRAPTRAGTW
                     WLVKAASGVLLVIFLGMHLVAQHFLVPGGLRDFASVVSYLRNPIALVAELGLLAAVVT
                     HAALGVRAVLVDVLPSAVAVRRASWVLGGAAIAAFAYGIGLTFAVIGS"
     gene            complement(9253..10071)
                     /locus_tag="VFV59_09110"
     CDS             complement(9253..10071)
                     /locus_tag="VFV59_09110"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5326025.1"
                     /translation="MTAAEEHDQGPRTWHHGLVARWWAEFNRADPDELAYFVAAVRRF
                     GEPALDLACGTGRILVPLLEAGLDVDGIDVSADMLRHAAAAAQAAGMRPTLLRQALHE
                     LQPPRRYRTIIICDSWGIGGSRSRDREALRRIHAALEPGGALVLSHDLPYEDAETWQR
                     WLPGSRSGLPDAWPTSGAPRPMADGTALDLRIRLEALDPAEQRLTLGIQARHWRDAEL
                     LSLEEMRIDIALYFRAELVEAIERAGFHDVEVQGWYTGQPALPDDARIVVVARA"
     gene            10127..10714
                     /locus_tag="VFV59_09115"
     CDS             10127..10714
                     /locus_tag="VFV59_09115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009342921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU5326026.1"
                     /translation="MGQMTTKGLELRPEQPGEEGAVRAVVADAFGGELEARLVDALRA
                     SPDWIDGLSFVAAVDRDVVGHVLFTRGLLDAPRRLVDVLVLAPVSVASAWQGRGVGSA
                     LIRHALTQVRDRAEPLVFLEGSPRFYPRFGFRAAGPLGFRRPSLRIPEPAFQVVRLPA
                     YEPWMTGTLVYSRVFWDLDCVGLREVSPGADTPAG"
     gene            complement(10683..12263)
                     /gene="aceB"
                     /locus_tag="VFV59_09120"
     CDS             complement(10683..12263)
                     /gene="aceB"
                     /locus_tag="VFV59_09120"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU5326027.1"
                     /translation="MTDTPPGVTLTRPTEHADEILTPDALAFVAELHRRFDPRRRELL
                     AARAERQARFDAGELPDFLVETASVREDDGWQVAPAPGDLLDRRVEITGPVEPKMMIN
                     ALNSGARVFMGDFEDALSPTWSNVVTGQWAVREAVRRRLTFETDEKAYRLNPEIATLV
                     IRPRGWHLDEANVLVDGSPISASLFEFGLVLFHNAREQLDRGSGPYLYLPKLESHLEA
                     RLWNEVFVWAQERIGIPHGSIRATVLIETILAAFEMDEILYELRDHAAGLNAGRWDYI
                     FSVIKKFRSRPDFVLADRAAVTMTVPFMRAYTELLVRTCHRRGAHAMGGMAAFIPSRR
                     DPEVNERAMAAVRADKERESRDGFDGTWVAHPDLVPLATEVFDAVVGDRPHQKDRLRP
                     EVHVSARALLDVRVPDGSVTDAGVRANVSVGLAYLASWLAGNGAAAINNLMEDAATAE
                     ISRSQLWQWRTHGVPLDDGRPFDADRYAAIRDEELAPLRRRYPEAPWTDAANLLDQLV
                     LADDFVEFLTLPAYPLLG"
     gene            12397..12648
                     /locus_tag="VFV59_09125"
     CDS             12397..12648
                     /locus_tag="VFV59_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEU5326028.1"
                     /translation="MPLFLDEHEHIPGLTAAALADAHARDLEVGPRYGVSYLRYWYDE
                     ATGKVFCLVDAPTPEAANDVHREAHGLVADRLVAVTEGA"
     gene            complement(12658..15414)
                     /locus_tag="VFV59_09130"
     CDS             complement(12658..15414)
                     /locus_tag="VFV59_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5326029.1"
                     /translation="MNDAQLPSGTVTLLFTDIERSTQLLEELGDAYGSLLVEHHRIVD
                     AAARQHGGRRVDAAGDGLFYSFGTAGGAIAAAVEGQRELATHQWPGGADVRVRMGLHT
                     GEPMVAGTGYVGIDVHRASRICSAGHGGQILVSAALRALVGGQLPDGIALHDLGEHRL
                     RGLAGAERLFQVMGDGLPESFPPVRSLETLPNNLPRQLSSFVGRDREIDEAAERLGAT
                     TTLTLTGVGGVGKTRLALEIGARLVHAYPGGVWLVELASIDEGTLVADAIATALGLKQ
                     RAAMSPLATVAEAIGERTTLLILDNCEHLLDAVVATADGLLRSCPRLKLLATSREALG
                     LPGESLMPVPSLSLPPGRPGADPADALAEYDAVRLFVDRARAALPDFALTEENAAAVS
                     QICRRLDGMPLAVELAAARIRSLPPQQIAARLDDRFRLLTGGSRTALPRHRTLRAAFD
                     WSYDLLTPEEQALVPRLSVFNGSFSLEAAEEVCSGGIVEREAMLDLLGRLIERSLLVG
                     DEGGVEARYRMLETIRDYAQERLAEQENAAEYHVRHLQWFVGLVEQASPAFFSGPVKA
                     EWVERLARDEENLRAALRWADEDPNGADAELALVSGLWRFWEVRGELLEGRTWLERGL
                     ARLGGEVSQRRSTALTGAGVLAAHRGDYAAAAAFHDASLLIQREIGNPWAVAAACSNS
                     ANVAVERGEFERARQLFEEALVLTRRAGDEHGAAFIITNLADVAARQGQPDEAERLYA
                     ESITAHDALGDRFGVAHATAQLAGLVRRRGDRDRASALLRQALAIHSETGDRRAEARI
                     HQNLGDLAAEAGDYATAEREYQWSLAIRATLGARVGIAAALERLAGVAEDRPERAALL
                     LGAAGALRDEIGTPLGAENRRRVEEFVAQLMTARGEAPIRRALAEGRMSPLAVVLDRA
                     ARRD"
     gene            complement(15568..16176)
                     /locus_tag="VFV59_09135"
     CDS             complement(15568..16176)
                     /locus_tag="VFV59_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEU5326030.1"
                     /translation="MIGSHLDARIFASVADFRAWLADHHDRARELWLGFYKKGVPKQA
                     MTYVEAVEEALCWGWIDGLTRGLDDVTRAIRFTPRRRTSNWSASNLERVARLAAAGRM
                     QPAGRRAFEERDPRRDATYSYEQAPADLPDDLLERFRADERAWEAWSTSAPSYRRTAI
                     HWVTSAKRPETRERRFAQLLDAHRAGTRPRPFLVSREQRGAS"
     gene            complement(16173..16385)
                     /locus_tag="VFV59_09140"
     CDS             complement(16173..16385)
                     /locus_tag="VFV59_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326031.1"
                     /translation="MSWGRFLAVGVLTMLLLIPLTPVVSAVSSPFDEPARTVVIVAVA
                     VAVFAAINQLLTWGLAAWERRERRQP"
     gene            complement(16391..17260)
                     /locus_tag="VFV59_09145"
     CDS             complement(16391..17260)
                     /locus_tag="VFV59_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5326032.1"
                     /translation="MPNAVEPISASPTRLADAYALLAEEGPAWRPIAGGTDVMVQLAG
                     EIGEPPDRLLDLWALDELRGIRVDGDALVIGALTTYTELRRSPVVARTLPVLADVAAT
                     IGAAQIQNRGTIGGNVMNASPAGDTLPLLLAVDAELVLGSSGGERHVAAAEFWPSYRT
                     TAARPDELLLRIRIGLESDRQVRFRKVGTRRAQAISKVAMALSWRADGTSGPWGDVHV
                     ALGSMAATPVRAPRTESAIEGRPPTRETADAAAAALLAEVSPIDDVRSTADYRRAVAA
                     RVLHRLVRDAGGW"
     gene            complement(17279..17416)
                     /locus_tag="VFV59_09150"
     CDS             complement(17279..17416)
                     /locus_tag="VFV59_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326033.1"
                     /translation="MTEHPESLHEAIEDRDVTLAMTPGQLLLLVVGVWLLLRFLRGLR
                     R"
     gene            complement(17413..17880)
                     /locus_tag="VFV59_09155"
     CDS             complement(17413..17880)
                     /locus_tag="VFV59_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5326034.1"
                     /translation="MTFRFTVNGAAVEVEAAGSRRLLDVLREDLALTGTKEGCGEGEC
                     GACSVLLDGAVVDSCLVPVSQANGASIQTVEGLAPDGRLGTLQQAFLETGGAQCGICT
                     PGMLIAGHAFLVSGAAPTEEAIREAIAGNLCRCTGYTKIIDAIALAAERSRTP"
     gene            complement(17877..20273)
                     /locus_tag="VFV59_09160"
     CDS             complement(17877..20273)
                     /locus_tag="VFV59_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5326035.1"
                     /translation="MERPSLTVRTPAPGVASPDAPAVLTGTAPVVGRARPAAPLRREG
                     PAKVTGEAKYADDLVVPGAWYGATIRSSEPHARLLGVELDDAFEWKRVVVVTADDIPG
                     DNVVSLIDDDQPVLVPIGGEIRHQAEPCVLIAAPDRATLREARLHVTLRTERLPAVFD
                     PLESDVEFAHYTVAQGDPDTAMAAADLVIEGTYRVGHQEQLYIENQAMIAVPRDDGGM
                     TVHGSLQCPYYIHKAMKRALNMGDELAVVVQAETGGGFGGKEEYPSIIALHASLLALK
                     CHKPVRMIYDRHEDISATTKRHPAVIRSRWGVSADGELLAQEIEVIMDGGAYCTLTPV
                     VLSRGTLHAGGPYRCPNVRITGRAMATNTPPNGAFRGFGAPQTEFAAEMQVNRLAEAL
                     DVSPLDLRRRWVYREGDTTPTGQVLRESVGGIDVLEAAASAAGFDRVEAQTRAMREQR
                     TDGARTATGIGLALAWHGAGFTGSGEVHLASVASVELTDDGRIRILTASTEMGQGTKT
                     IFPQLVADTLGVEYDEVEIAPQDTSIVPDSGPTVASRTAMVVGGLLIRAAEKLRAAVE
                     GRTGRPFRESYRDAGPLRVDEQFTPYPGEPFDDKRYRGDAYPAFGWAAAVVKVDVDLD
                     TGDVAVREYTGAADIGRVIHPVLAEGQVEGGSLQAVGYATIEEMKLVDGRYLNDRLAT
                     YLIPTSLDAPRIQSILVEKPFSGAPHGAKGVGELPMDVGAPAVVAAIHAATGAWIHEL
                     PATSERILAALTGGPMPPPPGVSLEAPAPRTPPIEDRPPGGDEIPLRAPTDPPQDFRP
                     "
     gene            20332..20781
                     /locus_tag="VFV59_09165"
     CDS             20332..20781
                     /locus_tag="VFV59_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326036.1"
                     /translation="MSFFEKARQTATQGLSQLTGGEGDSESRVTAESVTASVRELASH
                     AKRGVVTVIEKIDPDTLADLIIKATAIQEKANAALARKKAAYRIGDLSITATIPPQIG
                     FSIVRIGDVEEAIDEAALLESSQLEERIPTAEEAVISLDGETVPTDG"
     gene            complement(20791..>21025)
                     /locus_tag="VFV59_09170"
     CDS             complement(20791..>21025)
                     /locus_tag="VFV59_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU5326037.1"
                     /translation="TSAPAQRIGLVDRGIVSDGAVADLVIFDPDAVRATCTYEEPCSY
                     PEGIGWVIVNGEVVVREGVHTGARPGRVLRRGR"
BASE COUNT         2837 a         7508 c         7700 g         2980 t
ORIGIN      
        1 ctcgtcgcgg gcgaagtcgg cgaacgcgga gccggtgatc ttgatgagtt gcagcccttc
       61 gcgcccggac tcgtggtcgg tgatgcggac ggccccgtcg gcgtcgcggg cgagctccag
      121 gcgggcggtg ccgcggtcgc gtcggtcccg gctgaagagc cgatcggagg cggcgaagcc
      181 gtcccggtcg tcggccggca cgacggccgc ggccaggtcc gccacctgct cggcggccac
      241 gtagcgcgcg ggctcggcca gcaccgcgtg cgacgcatcg gtatagcgcc agctgatgtc
      301 cagccagatg tagagcggtc ggtcgcggcg ctcgggcagg gtggtgtgct cgtcgcgggc
      361 gaagtcggcg aacgcggagc cggtgatctt gatgagttgc agcccttcgc gcccggactc
      421 gtggtcggtg atgcggacgg ccccgtcggc gtcgcgggcg agctccaggc gggcggtgcc
      481 gcggtcgcat cggtcccggc tgaagagccg atcggaggcg gcgaagccgt cccggtcgtc
      541 ggccggcacg acggccgccg ggaagggcag ctcgcggccg tgcatggtca ggcgctccat
      601 gtgcgggtag gtgtccagga aggcggtgcc gacctcgagc agccactcct cgagcgtccg
      661 ggctcgggcg cggagcgacg tggcgtggat gaagttcttc atcgtgtcgg tcgccacgac
      721 cttccggttg tcgcccgtcg tgtaggcgtc caggaactcc gtgccatgga cgacgacctc
      781 gatctccgcg gcggccaggg tgttgtcgcg ccccgcgaac ggcgactcgg ggatcggcgt
      841 cacgccggtc agcggctcgg cgtaggtgcg atacaccttc acgtcagcct tgccgtaacg
      901 gatctcgtgc ttcatgggcc gggagtgtac cggccgattg tccggcccgg gccattcgtg
      961 cagcggtgga tccgctcagc ccggcatgga agcctggcca cgcagcgtcg acagccgatc
     1021 ggcggcgatg tccaccacgt cgtccagccc gcgccgcagc tcgccctccc gctcgtcgcg
     1081 cagcgcgcgc tccaggagcg gcaggatctc ctcccgcgag cggccggcga cgaacaccac
     1141 gaagcggaag ccgaagaccc gctcgtaggc gtcgttcagg gcttcgagct cctcggccac
     1201 ccagggcggc gtgggtggcg catcgggctg gtcgtagccc tgctcggcgt gtgagaggac
     1261 gctcaccgcc gcggggtccg ccccgatccg tgggtgtgcg ttcagcagct ccacctgctc
     1321 gtcctcgggg acggtgcgtg cgagctcctg ggcgcaggcg atcaggccgg cgtcgctcac
     1381 gaacgggcga gcggcggcca ggcggtcgag gaacctgggg gcgtcctcga acagcgtgcg
     1441 aagcaggtcg gccgccggct cggcgggcag cgcatcgagg tccgcgatgt cggaaaacgg
     1501 cgcgaacgga aggcgatcgc ccgcatcggt gcgaagctcg agctcgacgt agtcgcgcac
     1561 gggaaggccg ttctcggggt tctcgcgcgg gatctccggc ttgatggcgg agcggtccgc
     1621 gtcgacaccg ccggagtgca ccgcggcgac gtgcatgccg cgacgcaggt accagcggat
     1681 ggcgtcgacg ttgtcgttcg tcgtcaccag ccagagtcgg ggagcctccg acgccacggc
     1741 gacgcgcagc gccgtttcga gcaggcgcgt gcccgcaccg acgccgcgca cctcgctgtg
     1801 caggctgagc acctccaggc ccttctcgga ctcgctcacc gtgagcagcg cgaccggccg
     1861 gccgtccagc tccgccacga tcccctcgtg ctccagcggg tcgaggagct cgccggagcg
     1921 ggccacgtgg ggcacgccgc caccatgcgc gccgaggagc gcggcggccc aggcgcggtc
     1981 ctcgggcgcg aagtcccgga cgtcgagggg caccggcgtc agctgccgcg gtaggtcgtc
     2041 aggccgaagg ggctgagcag caacggaacg tggtacgacc gatcgatctc gacgacgcgc
     2101 agggccagcg ccacgctctg gaagaagtcg tcctcctcgt cggcgtagcc gccgacgtcg
     2161 aacgccagct ggtactcgcc ggccaccagc tccgcgccgt ccagcagatc gcgaatgcgg
     2221 ccgtcggcat cggtctcgag ctcgctcacg agctcgggcg tgcccgaggg gtccaggcgg
     2281 aacagggtca cggcaatccc ctcggcgggg tcgccggtcg cgagatcgag gacgtgcgtg
     2341 ctgagggtgg ggcgcgccgc tggcatcggt tcctccgcta tgctcgccac ccatgatgac
     2401 ggatgcgccc gttggtcgcg acgcccgtgt cgatctgctg ctgaccgggt cgctgcgtcc
     2461 ccgcgtgcga agcagctgca cgctggttcg cgaaggcagg gtggccgcgg tcgtggaccc
     2521 cggccttgcc ccgtcggccg ccgccatcct ggacccgctg cgagccctcg ggctgacccc
     2581 cggcgacgtc accgccgtgg tgctctcgca ccaccacccg gaccacacgc tcaacgccgc
     2641 gctcttcccg aacgcggagg tccacgacca ctgggcggtc tatcgcggcg acgactgggt
     2701 gacgcgggag tgcgagggcg cgcagctcag cccgtccgtc aggctgctcc gcacgcccgg
     2761 gcatgccgcg gaagagctgt cgaccgcgat cggaacgccc gacggcgtga tcgtcgccac
     2821 ccacctgtgg tggacggccg acggaccggc cgatgacccg tacgctcccg accgcgacca
     2881 gcttcgacgt tctcgcgaac gcgtgctcgc catcgccgac cggatcattc ccggccacgg
     2941 cgcaccgttc cgccccggag acgccacccc gcgctgacgg gcggcgctat cctccgccga
     3001 tggcagacct gcacatccgc gtcggtgacc tggccttcac cgccgactgg gaggccgatg
     3061 cgccgcgcac gcgcgacgcc ctccgcacgt ggcttccgat tcgctcgaag ctgatccact
     3121 gccgctggag tggcgagtcg acctggatcc cgttcggcga ccgccggccg gacgtggggt
     3181 acgagaacca cacctcccat cccgcgccgg ggcaattgct gatctacacc ggcgagatct
     3241 ccgagtgcga gatcctcttc ccgtacggcg cctgcatgtt cagctcgaag ctcggccagc
     3301 tggcgggcaa ccatttcgcg acgctggtga gcgggcagga gcagctcgcc gagctggggc
     3361 ggcgcgtgct gtgggagggc gcgcaggaga tcgagatctc cgagacggcg tgaccgatct
     3421 cgtcgtccgg ggcggcaccg tcgtcacggc cgagggcagc gtcgcggctg acgttgccgt
     3481 caccgacggc cgcatcgtcg ccgtcggccg gggcgccggc gcgggtgcgt tccgcgtgat
     3541 ggatgccgcc ggcctcctcg ttctgccggg cgccatcgac gtccataccc acctgcggct
     3601 gccggtgccg gagcgccccg accgcttccg ggccgatacg ctcgccgcgg ctcgcggtgg
     3661 gacgaccacg gtcgtctcct tcaacaatcc ggggaccggc atcagcgagg agggcgcggc
     3721 gtcgccgctc gccgggctcc gcgagttccg cgaacgcacg gcgggggagt cggccgtcga
     3781 ctacgccctc tcggccgtca tcggctcgca ccagccggac ccgctgcggg agatcccgga
     3841 gctgatcgcc tcgggcgttc cgaccttcaa ggccttcatg gtctacgact tcgccctgcc
     3901 cgatgaccag attcgggagc tgatgggcct cgccgcacgc cacggcggga tgctgcagct
     3961 gcactgcgag gagccagcga tcatcgagcc gctcatcgcc gatgcgctgg cgcgcggcga
     4021 caccggtcca cgacaccacg cccacacgcg gccgccgcac gcggaggcgg cagcggtgca
     4081 gaaggcgatc gcgtgggcgc gtcaggcgga ggcgccgctg tacatcgtgc acctctcctc
     4141 ggcggcggcc ctcgagcacg tggcggacgc caagggccgg ggggagccgg tgtatgccga
     4201 gacgtgcccg cactacctca ccgcaacgga cgacgtctac gccgatccgg acgaccggga
     4261 agtgatgaag cgcgtgatct ccccaccgct gcgctcccag gccgacgtgg aggcgctgtg
     4321 ggcggggctc cgcgacggcg tcctcgacgt gatcggcagc gaccacgtgc cggatcggct
     4381 cgacaccgag aagcggctgc cggccccacc gttccccgag atcagcaacg gcgggccggg
     4441 ggtcgagaca ctgctctccg tcgtctggtc cgaaggcgtc gccgcgcgcc ggatctcgcc
     4501 cgcccggctg gtccagctgc tcgcgaggaa cccctcgcgg ctattcggcc tgccgtccaa
     4561 gggcgcgatc caggtgggcc gcgacgccga cctggtcgtg tgggaccccg aggcgcgccg
     4621 cacgatccgc caggcggacc tccaccactc cagcgacttc acgcccttcg aggggctcga
     4681 ggtggtcggc gcgccggtcg aggtgctgct gcgcggtgag ccggtcgcgg aggctcgagg
     4741 ccggttcctc gaacgtcggc tgtgagggcg caggaatgac gaacgacccg gtggccttgg
     4801 ccaccgggtc gtatcggttc gcgagtggtc gcgcctagcg gcggaagcag tcgctgcagt
     4861 agacgggctt gctgccggtc gggcggaagg gcactcgggc ctccttgccg cagctgtcgc
     4921 aagtggcgct gaacatctcg cgcgggcggt cgctgtagcc accgccgtag ccgccgccgc
     4981 cgccaccgta gccgccggca ctgtcaccgc gctgggcctt gcgggaggcg cggcaggccg
     5041 ggcatcggcg cggctcgctg aactggcgct cggcgtagaa ctcctggtcg cgggcggtga
     5101 acgtgaagtc catgccgcag tcctggcagt tcagcgtctt gtcctggaag gtcaacaaag
     5161 gaaaacactc cttctcgtgc ggcccgagcg agccatctcg ccgaagaccg tgagaggagc
     5221 gttgctgtgc cgcggttggt tgtggcccca cgtggggcat ctgtcgcagg ggacgatacc
     5281 acggattcgg tggcgcgtca gtcccttgac gagacgaagc cgattcggtc gagtggcagg
     5341 tatcgaacca gggcacgccc gatgagccga tcgagcccga tcggtccgaa cgtccgactg
     5401 tccaggctgt cgctacggtt gtccccgagc acgaacaccg aaccttccgg gactcgccac
     5461 tcgcggctcc cggccggccc ctgcggcagg gtcaccgtgc cgtcggtgag gtacggctcg
     5521 tccaggcgaa acggtggcgc gtcggggccg ctgacgtaga tgcggccgtt ctcgatctgg
     5581 atggtctcac cgggcaggcc gatcacgcgc ttcacgtacg gcacgccatc gtcaccgtac
     5641 ggggatggcg cgtcgaacac gacgacctca ccgcggtccg gcgcgtccca gcgcagcgac
     5701 agccggtcga cgagaaggtg gtccccctcc accagcagcg gctgcatcga gcgcatctcg
     5761 acctcgaccg gctgcacgac gaaggtattg agcacgaggt acagcgccgc cacgaacagg
     5821 acggtgcgcg caacctcgag cacccagcgc cggatgtccg ccggccatcc gggaaccagc
     5881 ggctcgggaa ccgggttcgg ctcgggcggg cgaatcatcg gtcgtggatg tgacgcccgt
     5941 ggtgtcggcc gcgttgcgct cgtcgagact gaagcggctc ttgaatgccc gcgtatgctg
     6001 agcgcatggc acgcgacgag aagctcgacc acctgcgacg ggtcccgctc ttctcacgca
     6061 tgagctcgcg ggagatcgag cgactcgggc agctcgccga cgaagtgacc gttggcatgg
     6121 accaggtcct cgcgcaggag ggacggtacg ccgaggagtt cttcatcgtc cttgacgggc
     6181 gggtcaccgt gctcgacggc ggacgtccca tcgcggagct cggcccgggc gacttcttcg
     6241 gcgagatcgg gctgctggac gggcatcgtc gcacggccac cgtccgcgcc gacggcatca
     6301 cgcgcctcct cgtcatcggc caccgcgagt tcaacgcgct gatggacgag ttcccctcgg
     6361 tgcgcgaggc cgtgctgctc gcggtcgagg accgcgtcgc gcgctctcga gggaccgccg
     6421 cgagctgacg ccggtggccg aatagaccat cccaaacggt cgggatggcc cgtcccccgg
     6481 accgccacga gcccggatgc ttggcgtcga tggcaggaac gccgaacgtg ctggtgatcg
     6541 ggtccggctc gaccgggtcg gcggtcgcgc acgacctcgc gctgcgcggc gtcgcggtga
     6601 ccgtggtgga gcgcggcgag atcgcgtccg gcaccacggg tcgcaaccac ggcctgctgc
     6661 attccggcgg gcgctacgcg gtcaaggacg ccgagtcggc gatcgagtgc atcaacgagc
     6721 gggacatcct cgccgccatc gcccccgagg tgctcgagct gaacggcggc ctgttcgtcg
     6781 cgctcgacga ggacgacctc gcctatcgcg agccgttcct tcaggcctgc gccacgtgcg
     6841 gcatcccgac ccgcgagctc agcacgcagg aagcgcgcga gctcgagccg gcgctcagcg
     6901 ctcgcgttct cgccgcgatc gaggtcccgg acggcgtctt cgacccgctg cacctgtgcc
     6961 tcgccttcct cgccacggcc cagggcaacg gcgcccgggt gcgtgcctac accgaggtgg
     7021 aggagctgat cgtgcggggc tcgagcgtcg agggcgcgat cgtccgagat cgccggacgg
     7081 gtgagcgccg acgcgtggat gccgacctgg tggtgaacgc cacggggccc tgggctgacc
     7141 ggatcgctgc catggcgggc ctcgacgtgc cggtcgtccc gacggcgggc gtgatggtcg
     7201 cgatggccgg ccgcttcgtg gaccgcgtca tcaaccggct caacaagccg tcggacggcg
     7261 acatcgtcct gccccagcgg cggaccgtcg tcatcgggac ctcgtcgtgg caggtcgaga
     7321 accccgacct ggtcaccatt ccggccgacc acgtggcgct catggccgag cgagggggcg
     7381 agatggtccc gagcgtgcgc acggcgccga tgcgaggcgt cttcgccgcc tcgcgaccgc
     7441 tcatcgggcg gcccggggac accgacggcg ggcgggagct gtcgcgcacc ttcgagtgct
     7501 tcgaccacgc ccgcgagggc gtcagcgggt tcgtgaccat cagcggcggc aagacgacca
     7561 cggcccgggc gatggccgag aagacctcgg acgtcgtctg cgagcagctc ggcgtctcgg
     7621 ccgcctgtcg cacggaaacg gttcccctgc gctcataccg ggacttctac cgtggctgag
     7681 cagacggtga ccgcccgcgt ctggcggcag gcgggtcccg gcgcccgcgg agggttccgg
     7741 tcgtacgagg tcggggtgga tccggcggcc gactcggtgc tcgacgtgat cgagcggatc
     7801 tgggccgagc gcgaccacag cctggtcttc cgccacgcct gtcaccacgc gtcgtgcggg
     7861 acctgcgccg tggtggtcga tggacgcgag cgactgccgt gcatcacgcc gctgtcggag
     7921 gtgtgggacg gccgcagccc gatcacgctc gagccgttgc gcaacgtcga gctcgtcgcc
     7981 gatctcgcga tcgatcccgc gctcgcgctc cagcgcctgg ccgaactgcc cctgccgtac
     8041 gtccggaccg tcgaggccgc cgtgcgcgct gcctccggcg tggagcgcga tccggatgtc
     8101 ggggcggccg agcgcttcga ggactgcctg gagtgcctgg cctgcgtctc cgcctgcccc
     8161 gtggcggcgg gggatccgga gtacctgggg cccgcggtgc tggcggccgc gcaccgggcc
     8221 gtcgaggagc cccgcaacgg tggaaccgca cgcatcctgg ccctggtcga cacggaccat
     8281 ggcgtgtggc agtgccggtc gatctgggcc tgcagcgccg tgtgcccgtc ggcggtcgat
     8341 ccggcgcagc ggatcatgga cctccgccgc cgcctgtggg gccggcgccg ggaggcggcg
     8401 catggctgag cgcgggatgc cggtgcgcgc gcatcggccc ttcgcctggc tccagccgag
     8461 gtggcgcgac gcgggctggt gggccttcgt gctcaaccgg ctgaccgggc tgatcctggt
     8521 ggcctacctg ctcgcccact tcatggtgct gtcgctgctg acccagggtg cccggggatg
     8581 gaaccagctg ctggagctct tcggctcgac gccgttcctg gtcggtgacg tcctcctcat
     8641 ctcggccgtc gcgttccacg gcctcaacgg cctgcgggtc atcggcctca ccttcgggct
     8701 cggcgcgcgc cacccgaccc tgacggtctt cgtcgtcctg ggcatcagcg cggtgctcgg
     8761 cgcgctggtg ggctggctgg tgctgttcgc atgagcgcgc cgattcccgt ccgccgcttc
     8821 ccgggccgtc cggacgcggc cccgccgggc ttcgtccccg gggccgagcg tctcgagccc
     8881 accatcgagg agctgcgcgc acccacccgt gctggaacgt ggtggctggt gaaggcggcg
     8941 agcggcgtgc tgctggtcat cttcctcggc atgcacctcg tggcgcagca cttcctggtc
     9001 cccggcggcc tgcgggactt cgcgtcggtc gtgtcgtacc tgcgcaaccc catcgccctc
     9061 gtcgcggagc tcggcctgct cgccgcggtg gtgacccacg ccgccctcgg ggtccgcgcg
     9121 gtgctggtcg acgtcctgcc gtcggcggtc gccgtccggc gggccagctg ggtgctgggc
     9181 ggtgcagcca tcgcggcgtt cgcctacggg atcgggctga ccttcgccgt catcggttcc
     9241 tgatccgctc cgtcacgcac gggccacgac gacgatcctg gcgtcgtcgg ggagcgcggg
     9301 ctgccccgtg taccagccct ggacctcgac gtcgtgaaat cccgcccgct cgatggcctc
     9361 gaccagctcg gcccggaagt agagcgcgat gtcgatgcgc atctcctcga ggctgagcag
     9421 ctcggcgtcg cgccagtgcc gcgcctggat gcccagcgtc agccgctgtt ccgccggatc
     9481 gagcgcctcg agccggatcc ggagatccaa cgcggtaccg tcggccatcg ggcgcggcgc
     9541 accgctcgtc ggccacgcat ccggcaggcc cgaccggctg ccggggagcc accgctgcca
     9601 cgtctcggcg tcctcgtacg gaagatcgtg gctcaggacc agagcgccgc cgggctccag
     9661 cgcggcatgg atccggcgca gggcttcccg gtctcgcgat cgactgccgc cgatgcccca
     9721 ggagtcgcag atgatgatgg tccgatagcg tcgcggcggc tgcagctcgt gcagggcctg
     9781 ccgaaggagc gtcgggcgca tgccagccgc ctgtgcggcg gcggccgcgt ggcgcagcat
     9841 gtcggccgag acatcgatgc cgtcgacgtc gagcccggcc tcgagcagtg ggaccaggat
     9901 gcgccccgtc ccgcaggcca ggtccagcgc gggctctccg aagcgccgga cggccgcgac
     9961 gaagtaggcg agctcgtcgg ggtcggcgcg attgaactcg gcccaccagc gggccaccag
    10021 gccgtggtgc cacgtgcgcg ggccctggtc gtgctcctcc gccgctgtca cgtgccgatc
    10081 ctaggagcag ccgaccccgc gcgtcgagcc tgtcacactg gcgtccatgg gtcagatgac
    10141 caccaagggg ctcgagcttc gcccggagca gccgggcgag gaaggagcgg ttcgcgcggt
    10201 cgtcgccgat gccttcggtg gcgagctgga ggcccgattg gtcgacgcgc tgcgggcctc
    10261 ccccgactgg atcgacgggc tgtcgttcgt ggctgcggtg gaccgcgacg tggtggggca
    10321 cgtcctcttc acccgcggat tgctcgacgc tccccggcga ctggtcgacg tcctggtgct
    10381 ggccccggta tcggtggcga gtgcctggca gggacgtggc gtcggcagcg cgctgatccg
    10441 ccacgccctg acgcaggtgc gggaccgtgc ggagccgctc gtgttcctcg aaggatcccc
    10501 gcgcttctac ccgcgattcg ggttccgggc cgccggtccg ctggggttcc gccgcccgtc
    10561 gctccgcatc cccgagccgg cattccaggt cgtgcgcctg ccggcatacg agccatggat
    10621 gacggggacg ctggtgtact cgcgcgtctt ctgggacctc gactgcgtcg gtctgcgcga
    10681 ggtcagccca ggagcggata cgccggcagg gtgaggaact cgacgaagtc gtcggcaagc
    10741 acgagctggt cgagcaggtt ggcagcgtcc gtccacggtg cctcggggta gcggcgccgc
    10801 agcggggcca gctcctcgtc gcggatggcg gcatagcggt cggcgtcgaa cggccggccg
    10861 tcgtcgagcg gcacgccatg ggtgcgccac tgccacagct gggagcggct gatctcggcg
    10921 gtggcggcat cctccatcag gttgttgatg gccgccgccc cgttgccggc cagccaggag
    10981 gcgaggtacg ccaggccgac cgagacgttg gcccgcacgc cggcatcggt gacggagccg
    11041 tcggggacgc ggacgtccag gagcgcccgg gccgagacgt ggacctcggg cctcagccgg
    11101 tccttctggt gcgggcggtc gccgacgacg gcgtcgaaga cctcggtcgc caggggcacc
    11161 aggtccgggt gcgcgaccca ggtgccgtcg aaaccatcgc gcgactcgcg ctccttgtcg
    11221 gcgcggaccg cggccatggc gcgctcgttg acctcggggt cgcggcgcga ggggatgaac
    11281 gccgccatgc cgcccatggc gtgcgcgccg cggcggtggc aggtgcgcac cagcagctcg
    11341 gtgtaggcgc gcatgaacgg gacggtcatg gtcaccgccg cacggtcagc gagcacgaag
    11401 tcgggccgcg agcggaactt cttgatgacg ctgaagatgt agtcccagcg gccggcgttc
    11461 aggccggcgg cgtggtcgcg cagctcgtag aggatctcgt ccatctcgaa ggcggccagg
    11521 atcgtctcga tcaggacggt cgcccggatc gagccgtgcg ggatgccgat gcgctcctgc
    11581 gcccagacga acacctcgtt ccacaggcgc gcctcgaggt gcgattcgag cttcggtagg
    11641 tagaggtacg ggccgctgcc tcggtccagt tgctcacgcg cgttgtggaa cagcacgagg
    11701 ccgaattcga agaggctggc cgagatgggc gagccgtcga ccagcacgtt cgcctcgtcg
    11761 aggtgccagc cacgcggccg gatgaccagc gtcgcgatct ccgggttcag gcggtaggcc
    11821 ttctcatcgg tctcgaaggt caggcgacgg cggacggcct cccggaccgc ccactggccg
    11881 gtcacgacgt tggaccaggt gggggagagg gcatcctcga agtcgcccat gaagacccgc
    11941 gcgccggagt tgagggcgtt gatcatcatc ttgggctcga ccgggccggt gatctcgacc
    12001 ctacggtcca gcaggtcccc cggggcgggc gccacctgcc acccgtcgtc ctcgcggacc
    12061 gatgcggtct cgaccaggaa gtccggcagc tcgccggcat cgaagcgcgc ctgcctctcg
    12121 gcgcgtgccg ccagcagctc acgtcggcgc ggatcgaagc gccggtgcag ctcggccacg
    12181 aaggcgaggg cgtcgggcgt caggatctcg tcggcgtgct cggtcggccg ggtcagggtg
    12241 actccggggg gcgtatcggt catgcgcgaa gggtagtcgg cggatccggc gcacgcccgg
    12301 caggtttcgg tgtcgcatat gcgacaccat ctcttgcgcg cggggactac gatcgggtcc
    12361 attgatcggc gccgcacggg catggaggtc tcacgcatgc cgctcttcct ggacgagcac
    12421 gagcacatcc ccgggctcac cgcggccgcg ctggccgacg cgcacgcccg cgacctggag
    12481 gtcggacccc gctacggggt cagctacctg cgctactggt acgacgaggc gacgggcaag
    12541 gtcttctgcc tggtcgacgc gccgacgccg gaggccgcga acgacgtcca ccgcgaggcc
    12601 cacggcctcg tcgccgatcg cctggttgcc gttaccgaag gcgcgtaggc cccgccccta
    12661 gtcgcgcctg gcggcgcggt cgaggacgac agccagcggc gacatccgcc cctcggccag
    12721 cgcccggcgg atcggggcct cgccccgggc cgtcatgagc tgcgcgacga actcctccac
    12781 gcggcgtcgg ttctcggcgc ccagcggcgt cccgatctcg tccctcagcg ctcccgcggc
    12841 gccgagcagc agcgccgccc gctcgggacg gtcctccgcg acgcccgcca ggcgctcgag
    12901 cgcagccgcg atccccaccc tggcgccgag cgttgcccgg atggccaggc tccactggta
    12961 ctcgcgctcc gcggtggcgt agtcgccggc ctcagccgcg aggtcgccca ggttctggtg
    13021 gatccgggcc tcggcgcgcc ggtcgccggt ctcgctgtgg atggccagcg cctggcgcag
    13081 gagcgcgctc gcgcggtccc ggtcgccacg gcgccggacc aggccggcca gctgcgcggt
    13141 ggcgtgcgcg acgccgaaac ggtcgccgag cgcgtcgtgc gcggtgatgc tctcggcgta
    13201 gagccgttcg gcctcgtcgg gctgcccctg ccgggcggcg acgtcggcga ggttggtgat
    13261 gatgaacgcc gcgccatgct cgtccccggc acgacgggtg agaacgagcg cctcctcgaa
    13321 cagctggcgc gcccgctcga actcgccgcg ctcgacggcg acgttcgcgc tgttgctgca
    13381 ggccgcggcc acggcccacg ggttgccgat ctcgcgctgg atgagcaggc tggcgtcgtg
    13441 gaacgcggcc gccgccgcgt agtcgccacg gtgcgccgcg agcacgcccg cgccggtgag
    13501 ggccgtcgag cgccgctgcg acacctcgcc gccgaggcgc gccagtccgc gctcgagcca
    13561 ggtgcgtcct tccagcagct cgccgcgcac ctcccagaat cgccagagtc cggacacgag
    13621 ggcgagctcg gcgtccgcgc cgttcggatc ctcgtcggcc cagcgcaggg cggcacgcag
    13681 gttctcctcg tcgcgagcca ggcgctcgac ccactcggcc ttgaccggcc cggagaagaa
    13741 cgccgggctt gcctgctcga ccagccctac gaaccactgc aggtgccgga cgtggtactc
    13801 ggcggcgttc tcctgctcgg cgagacgctc ctgggcgtag tcgcggatgg tctccagcat
    13861 ccggtatcgg gcctcgacgc ccccctcgtc accgacgagc agcgagcgct cgatgaggcg
    13921 accgaggagg tcaagcatgg cctcgcgctc cacgatcccg ccgctgcaca cctcctcggc
    13981 agcctccagc gagaaggacc cgttgaacac cgagaggcgc ggcacgagcg cctgctcctc
    14041 gggcgtcagc aggtcgtagc tccagtcgaa cgccgcgcgc agcgttcgat ggcgggggag
    14101 cgcagtccga ctgccgccgg tgaggagccg gaagcggtcg tcgaggcgcg cggcgatctg
    14161 ctggggtggc agtgagcgga tgcgcgccgc ggcgagctcc acggccagcg gcatcccgtc
    14221 caggcgacgg cagatctggc tgacggccgc cgcgttctcc tcggtcagcg cgaagtcggg
    14281 cagcgcggcc cgtgcacgat cgacgaagag tcgcacggcg tcgtactccg cgagcgcatc
    14341 ggccggatcg gcacccgggc gtccgggtgg cagcgagagc gatggcacgg gcatcaggct
    14401 ctcgccgggc aggcccagcg cctcgcggct ggtggcgagg agcttgaggc gggggcagct
    14461 gcgcagcagc ccatcggccg tcgcgaccac ggcgtcgagc aggtgctcgc agttgtcgag
    14521 gatcagcagc gtggtgcgct caccgatcgc ctccgccacc gtggccagtg ggctcatggc
    14581 ggcgcgctgc ttgagaccga gggcggtggc aatggcatcg gcgacgagcg tgccctcgtc
    14641 gatcgaggcc agttcgacca gccacacgcc gccgggatag gcgtggacga gacgcgcgcc
    14701 gatctccagc gcaagccggg tcttgccgac gccgcccacg ccggtcaggg tcagcgtcgt
    14761 cgtcgcgccc aggcgctccg ctgcttcgtc gatctcgcgg tcgcggccca cgaagctcga
    14821 gagctggcgc gggaggttgt tcggcagcgt ctccagcgac ctgacgggtg ggaagctctc
    14881 cggcaggccg tcgcccatga cctggaagag ccgctccgcg ccggccagcc cgcgcagccg
    14941 atgctcgccc aggtcgtgga gcgcgatgcc atcgggcagc tggccgccga ccagcgccct
    15001 gagcgccgcc gagaccagga tctggccgcc gtggcccgcg gagcagatcc gcgaggcccg
    15061 gtgcacgtcg atgccgacgt acccggtgcc ggccaccatc ggctcgcccg tgtggaggcc
    15121 catcctgacg cgcacgtccg cgccacccgg ccactggtgg gtggcgagct cacgctgccc
    15181 ctcgacggcc gcggcgatgg caccgcccgc cgtgccgaac gaatagaaga gcccgtcgcc
    15241 cgcggcatcc acccgccgtc caccgtgctg gcgcgccgcc gcgtcgacga tgcgatggtg
    15301 ctcgacgagc agcgacccat aggcgtcgcc gagctcctcc agcagctgcg tcgatcgctc
    15361 gatgtcggtg aagagcagcg tgaccgtgcc gctcggcagc tgggcgtcgt tcacgtcgag
    15421 gggacggccg cgatgccgtg gggggcaggg aaccacggaa cgcggagcgg cgggttcacg
    15481 agcgacctcc gatcggggga cggatggggc agcagcatac gacctgcgcc atcaaccgcg
    15541 gccgcgagcg cagccgccaa cgcccggtca ggaggcgccg cgttgctcgc gcgagaccag
    15601 gaagggccgc ggccgcgttc cggcgcggtg agcgtcgagg agctgggcga accgacgctc
    15661 tcgcgtctcc ggccgcttgg cggaggtgac ccagtggatg gccgtgcgcc ggtaggacgg
    15721 agcggaggtg ctccatgcct cccaggctcg ctcgtccgcg cggaaccgct cgagaaggtc
    15781 gtcgggaagg tcggcgggcg cctgctcgta cgagtaggtc gcgtcgcgcc gtggatcgcg
    15841 ttcctcgaag gcacgccggc cggccggctg catgcggccc gcggccgcca ggcgcgcgac
    15901 gcgctcgagg ttcgacgcgc tccagttcga ggtgcggcgt cgcggcgtga accggatggc
    15961 gcgcgtcacg tcgtccagcc cacgggtgag gccatcgatc cagccccagc acagtgcctc
    16021 ctcaaccgcc tcgacgtacg tcatcgcctg cttcggcacg cccttcttgt agaaccccag
    16081 ccacagctcc ctggcccggt catggtgatc cgcgagccac gctcggaagt cggcgaccga
    16141 ggcgaagatg cgcgcgtcga ggtgggatcc gatcatggct gccggcgctc gcggcgttcc
    16201 caggcggcca gaccccaggt cagcagctgg ttgatggcgg cgaacaccgc cacggcgacc
    16261 gcaacgatga ccacggttcg cgccggctcg tcgaagggcg acgacacggc cgagacgacg
    16321 ggcgtgaggg gaatcagcag cagcatggtc agcacgccca ccgccaggaa ccggccccag
    16381 gacacggcgg ctaccagcct ccggcgtcgc ggaccagccg atgcagcacg cgagccgcca
    16441 cggcacgacg gtagtcggcc gtcgagcgca cgtcgtcgat gggggagacc tcggccagca
    16501 gcgccgccgc cgcggcgtcg gccgtctcgc gcgtgggcgg ccggccctcg atggcggact
    16561 ccgtgcgcgg tgcgcggacg ggcgtcgccg ccatcgagcc cagggcgacg tgcacgtcgc
    16621 cccacggacc gctcgtcccg tcggcacgcc acgacagcgc catggcgacc ttgctgatgg
    16681 cctgggcacg gcgcgtcccg accttgcgga atcgcacctg acggtcgctc tcgagcccga
    16741 tgcggatccg cagcagcagc tcgtcggggc gcgccgccgt ggtccggtac gacggccaga
    16801 actcggcggc cgccacgtgc cgctccccgc cggatgatcc gagcacgagc tcggcgtcga
    16861 cggccagcag caggggcagc gtgtcgccgg ccggcgacgc gttcatgacg ttcccgccga
    16921 tggtgccgcg gttctggatc tgggcggcgc cgatggtggc ggccacgtcg gcgaggaccg
    16981 gcagcgtgcg ggccacgacc ggcgagcggc gcagctcggt gtacgtggtc agggcgccga
    17041 tgaccagggc gtccccgtcg acgcggatcc cccgcagctc gtccagcgcc cacaggtcca
    17101 gcagccgatc cggcggctcg ccgatctcgc cggccagctg caccatcacg tcggtaccgc
    17161 cggcgatcgg ccgccacgcc ggtccttcct cggcgagcag ggcgtaggcg tcagccaggc
    17221 gggtggggga ggcgctgatc ggctcgacgg cgttcggcat cggtccggga tccgcggctc
    17281 agcgccgcag gccgcgcagg aagcgcagca gcagccacac gccgacgacc agcaggagca
    17341 gctgccccgg cgtcatggcc agcgtcacgt cgcgatcctc gatggcctcg tgcagggact
    17401 ccgggtgctc cgtcatggcg tgcggctccg ttccgcggct aacgcgatgg catcgatgat
    17461 cttggtgtaa ccggtgcagc ggcacaggtt gccggcgatc gcctcgcgga tggcctcctc
    17521 cgtcggcgcc gcgccggaga cgaggaacgc gtgccccgcg atcagcatgc cgggcgtgca
    17581 gatgccgcac tgcgcgccac ccgtctcgag gaaggcctgc tggagggtcc cgagcctgcc
    17641 gtctggcgcc agcccttcca cggtctgaat gctggcgccg ttggcctgcg agacggggac
    17701 caggcacgag tcgaccaccg cgccgtccag cagcacgctg caggcgccgc actccccctc
    17761 gccgcagccc tccttggttc cggtcagggc caggtcctcg cgaaggacgt ccaggagacg
    17821 ccgagagccg gcggcctcga cctcgaccgc cgcgccgttg acggtgaacc ggaaggtcac
    17881 ggccggaaat cctgcggcgg atccgtcggt gcacgcagcg ggatctcgtc accacccggc
    17941 ggccggtcct cgatcggggg cgtcctgggc gcaggggcct ccagggagac gccgggtggc
    18001 ggcggcatcg gtccgccggt gagcgccgcg aggatccgtt ccgacgtcgc cgggagctcg
    18061 tggatccagg cgccggtggc ggcgtggatg gcggcgacga cggccggggc gccgacgtcc
    18121 atcggcagct cgccgacacc ctttgcgccg tgcggcgcgc ccgagaacgg cttctcgacc
    18181 aggatcgact gaatgcgcgg cgcgtcgagc gaggtgggga tcaggtaggt ggccaggcgg
    18241 tcgttcaggt agcgcccgtc gaccagcttc atctcctcga tggtggcgta gccgaccgcc
    18301 tggagggagc cgccctcgac ctggccctcg gccaggaccg ggtggatcac gcggccgatg
    18361 tctgcggcgc cggtgtactc gcgcacggca acatcgccgg tgtccaggtc cacgtcgacc
    18421 ttgaccacgg cggcggccca cccgaaggcc gggtacgcgt cgccgcggta gcgcttgtcg
    18481 tcgaagggct cgccggggta cggcgtgaac tgctcgtcga cccgcagcgg cccggcgtcg
    18541 cggtaggact cgcggaacgg gcgaccggtc cgcccctcga ccgcggctcg cagcttctcg
    18601 gcggcgcgaa tgagcagccc gccgacgacc atggcggtcc gtgacgcgac cgtcgggccc
    18661 gagtcgggaa cgatcgacgt gtcctgcggg gcgatctcca cctcgtcgta ttcgacgccc
    18721 agcgtgtcgg cgaccagctg cgggaagatc gtcttggtgc cttggcccat ctccgtcgat
    18781 gcggtcagga tccggatgcg gccgtcgtcg gtcagctcga cgctcgccac ggaggccagg
    18841 tgcacctcgc cggagccggt gaaccccgcg ccgtgccagg ccagcgccag cccgatgccc
    18901 gtcgcggttc gggcgccgtc ggtgcgctgc tcccgcatgg cccgcgtctg cgcctcgacc
    18961 cggtcgaagc cggcagctga tgcggcggct tcgagcacgt cgatcccgcc gaccgattcg
    19021 cgcagcacct ggcccgtcgg ggtggtgtcg ccctcgcggt acacccagcg acggcgcagg
    19081 tcgagcgggc tgacgtccag cgcctccgcg agacggttga cctgcatctc ggcggcgaac
    19141 tcggtctggg gcgccccgaa gccccggaac gcgccgttcg gcggcgtgtt ggtggccatg
    19201 gcgcggccgg tgatgcggac gttcgggcat cggtacgggc cgccggcgtg cagggtcccg
    19261 cggctgagga cgacgggcgt cagcgtgcaa tatgcgccgc cgtccatgat cacctcgatc
    19321 tcctgggcca gcagctcgcc gtcggcgctg acgccccagc gcgagcggat gacggcgggg
    19381 tgccgcttgg tggtggcgct gatgtcctcg tggcggtcgt agatcatgcg caccggcttg
    19441 tggcacttca gggccagcag cgaggcgtga agcgcgatga tcgacgggta ctcctccttc
    19501 ccgccgaagc cgccaccggt ctcagcctgg accacgaccg ccagctcgtc gcccatgttc
    19561 agcgcccgct tcatggcctt gtggatgtag tacgggcact gcagcgagcc gtggacggtc
    19621 atcccgccgt cgtcgcgcgg gacggcgatc atggcctggt tctcgatgta cagctgctcc
    19681 tggtggccca cgcggtaggt gccctcgatc accaggtccg ccgcggccat ggcggtgtcc
    19741 ggatcgccct gcgccaccgt gtagtgcgcg aactcgacgt ccgactcgag cggatcgaag
    19801 acggccggca gccgctcggt gcgcagcgtc acgtggagcc gtgcctcgcg cagcgtggcc
    19861 cggtcggggg ccgcgatcag gacgcacggc tcggcctggt gacggatctc gccgccgatc
    19921 ggcacgagca ccggctggtc gtcgtcgatg aggctgacga cgttgtcgcc ggggatgtcg
    19981 tccgcggtca cgaccaccac gcgcttccac tcgaaggcgt cgtccagctc gaccccgagc
    20041 aggcgggcgt ggggctcgct ggagcggatg gtggcgccgt accaggcgcc cgggacgacc
    20101 aggtcgtcgg cgtacttcgc ctcaccggtg accttggcgg ggccctcgcg ccgcagcggc
    20161 gcggcggggc gggctcgtcc gaccacgggc gccgtgcccg tcaagacggc cggcgcatcg
    20221 gggctggcga cgccgggggc aggcgtgcgc acggtcaggc tcgggcgctc catcggctca
    20281 tgctagcgtg ccgcaccggg gccgattgac gtcctgcgta cagtggcgcc gatgagcttc
    20341 ttcgagaagg cgcgccagac cgcgacgcag ggcctgagcc agctgaccgg cggcgagggc
    20401 gacagcgaga gtcgcgtgac cgccgaatcc gtcacggcct cggtccgcga gctggccagc
    20461 cacgccaagc gcggcgtggt gacggtcatc gagaagatcg accccgatac gctggccgat
    20521 ctcatcatca aggcgacggc catccaggag aaggccaacg cggcgctcgc ccgcaagaag
    20581 gccgcctacc ggatcggcga cctgagcatc accgccacca tcccaccgca gatcgggttc
    20641 tcgatcgtgc gcatcggcga cgtcgaggag gcgatcgacg aggccgcgct gctcgagtcg
    20701 agccagctcg aggagcggat cccgacggcc gaggaggccg tcatctcgct ggacggcgag
    20761 accgtcccga ccgacggctg acgggaccgc tcagcgaccc cgccgcagga cgcgccccgg
    20821 ccgcgcgccg gtgtgcacgc cctcgcgcac gacgacctcc ccgttgacga tcacccagcc
    20881 gatgccctcg gggtacgagc acggctcctc gtaggtgcac gtcgcgcgga ccgcgtcggg
    20941 atcgaagatg accaggtccg ccacggcgcc gtcgctgacg atcccccggt cgaccaggcc
    21001 gatgcgctgc gcgggcgccg aggtc
//
LOCUS       DASTYP010000206        13236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1328436, whole genome shotgun sequence.
ACCESSION   DASTYP010000206 DASTYP010000000
VERSION     DASTYP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328436"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..363
                     /locus_tag="VFV59_09175"
     tRNA            287..363
                     /locus_tag="VFV59_09175"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:321..323,aa:Val,seq:gac)
     gene            749..1258
                     /locus_tag="VFV59_09180"
     CDS             749..1258
                     /locus_tag="VFV59_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5326038.1"
                     /translation="MRGDTAAFEELTVAYSPEMFRLAAAMVGPDDAADMVQEAFVSAW
                     RELPRLRDADRWSSWLRSILMNRCRNALRTRTRRPQLVLLDPDGHATPRQVAHEPMRD
                     VDRRWAMHDALGALRPEDRAVIVLHYLADLPLRQVASVLGLRLGTVKSRLNAGLRALR
                     AAGHDEELA"
     gene            1255..2358
                     /locus_tag="VFV59_09185"
     CDS             1255..2358
                     /locus_tag="VFV59_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326039.1"
                     /translation="MTALPPDAQRYLGRMRSLEPPVELVDSIMAEVEATPQVRSGSDL
                     RAMSTFVLAAAAVLLAIAVLLRIGAQDVGPAPTPVPLDQLPSAGSVQARISIEASDVP
                     AAFGHEFLWLTNATTGELVRMDPANGSIASPLQVTRAGGEVAIAISESSVWVADRRDA
                     TVVELDPATLEERRRIPVTATVSAVATLGADLWLLDTGAGQLVRFDTADAGAALTVPV
                     DGPSALLIHDGAVWVADELGELVRIDPASGDETGRVDLGFAVMRLVPDGGSLLAVGGA
                     ADLVARIDVGSMRVSARGVRAPAIAVEGGRVWAVTASGYLVSLDPATLQPLAASALEL
                     DASGTLAFGGGLLWTTGVDAAGDAYLLKVVPQP"
     gene            2451..3536
                     /locus_tag="VFV59_09190"
     CDS             2451..3536
                     /locus_tag="VFV59_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326040.1"
                     /translation="MRPSHAIVLAFLISGCSAAADATAAASASASMTVSAQSSPSPSA
                     GATEPDWSALPRLDESVTARVPITGGWSVLMGAAPVAYGSLWISNGSEAGEPPRLRRL
                     DRETMEVTATIDLGGAADVFPPDAYGAFYSAHGIWVPLMSQKAVVLVDPTTNSVSGRI
                     EVDATPYGLLEDGDDLWITDFGNSEVLRVDIPTGEERLRVSIPEPTGMASGPEGLWVI
                     EHETGFVTRLDPVTGEELARVQVGGRPGIVLGLGSVWASSSDEKTVSRIDPATNAVIA
                     TIVLPSHPSGAAIAGGSLWLTVGPQRGACERTSYLVRIDPATNEVDGILDVPCAGSPV
                     SDGARVWVNSGEEDAVWILSVEAGGRP"
     gene            3738..4514
                     /gene="yaaA"
                     /locus_tag="VFV59_09195"
     CDS             3738..4514
                     /gene="yaaA"
                     /locus_tag="VFV59_09195"
                     /inference="COORDINATES: protein motif:HMM:NF015820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU5326041.1"
                     /translation="MLIILPPSETKRPAPDEGSPLDLDALSFPELKPMRERVILALSA
                     TSQAPDALRRLFVGSTLAPEVARNKLMRHLPTRRAVDTYSGPLYVGLDAGSWSPEVEA
                     RAAHHVVIVSALWGALRPADLIPPYRLHVCARLVGMDRLEPAWRPLLEPILTDAAGRS
                     GPVLDLRSRLYQAVGRPTGLDDQTVTLRVRPAPGGPAHIGDVIAKRMRGEAAAHLLAS
                     ATLPSDPLDIADLLAERWSVEVDPPSVLDRSWTVTLRAAA"
     gene            complement(4446..5720)
                     /locus_tag="VFV59_09200"
     CDS             complement(4446..5720)
                     /locus_tag="VFV59_09200"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5326042.1"
                     /translation="MDLTLDDLALRAGVPVARIERLVEIGVIAPDAAGRFRPADVQRV
                     EIAGAYERGGISLDDLGGALREGRVSFAYSERIYPEGSVASGRTVADVAGDLRIDPTT
                     IADLFVALGLPFPEADHELREADVTAITRFVTAWGGEGMAADARDRAARLVGDAARRA
                     TEGWVNLFMEAIGLSIEERAEMRADELRTRLFEPGARVAEAFEPTMLWLLRRHMEHAL
                     NALNVESMERVLEHKGVRPTHAHDARGVVFADLTGYTRLTEEGGDAQAARHAATLAEV
                     ATGLAARHRGRLVKQLGDGVMLVFGDAGAAVAASADLMGRAGDRGLPPLHIGVSAGPV
                     VERDGDYFGRTVNLAARLSGIAGPGEVIVSGSVADDVDADRLEAIGSRELKGFEARIP
                     VYRLRLQPRRSGGGPEGHGPAAVEDARRIDLD"
     gene            5967..7799
                     /locus_tag="VFV59_09205"
     CDS             5967..7799
                     /locus_tag="VFV59_09205"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEU5326043.1"
                     /translation="MSQRRTRFMLPLVAAMVFTMVLVPSASAETLLDQSQEQATASSS
                     YIPNDEANAGLAQTFKAGLAGILHSIDLKLYKVNTPTAGLKVELHTGPPTGAVLATSD
                     EVGADKVGTDPAGAWISFRFATPATLVAGTEYSLVLAPAAADTNSADPTYVWFRSGAD
                     AYAGGTAWAASTSAAPIPWLQLSPAVDFAFHTYVTTAAPTCDVALALVDATDLVDDEL
                     SVVAGQKFEAWGFDYPPLTAVTVTLTPTSGTAITQAVSTDAYGEFAVEMPALDAAKVG
                     AWTATALPATGCPSTADAVTLVVRAARTVDRIFGADRFETAKKISAAWFAPATTDAAF
                     IATGMRFPDALAGGPAAAWEGAPILLSTQTALPTPTRDELLRLKPATIYVLGGTGAVS
                     AGVQTQLAALTRDGKATSVVRIGGADRYATAALISQTFFPKDAPAVMIATGRNYPDAL
                     AGAAAGARYGIPILLVTGDSIPAATAAELIRLNPDEIYVLGQTGAVTAGVQTQLAAYT
                     SSKTPASVIRLGGATRYDTAVAISQHFFPAGLSPVFVATGVNFPDALAAGPLGRAVLL
                     VPGTSVPINIANEVRRITPTDVHLLGGPLVVSDGVATTLKAVLP"
     gene            complement(7848..8303)
                     /locus_tag="VFV59_09210"
     CDS             complement(7848..8303)
                     /locus_tag="VFV59_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU5326044.1"
                     /translation="MDAIALLKDDHDRIKKLLAEGEETTERAEKTRTQLFDTIKEEML
                     IHERIEEEIFYPALKQHPKAKEIVLEGFEEHHVVDEVMGELEATGVTDERWAAKFTVM
                     KENIEHHIEEEEGDMFKTARQVFSNEELEQLGAKMGELKKLGQQVGAAD"
     gene            complement(8395..9306)
                     /locus_tag="VFV59_09215"
     CDS             complement(8395..9306)
                     /locus_tag="VFV59_09215"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEU5326045.1"
                     /translation="MAATAGTVCVVGSINVDLVVVAERLPRPGETVLGGRFETHDGGK
                     GANQAVAAARAGASVAFIGAVGTDANGDRALDALVAEGVDVSRVRRHATDPTGVALIA
                     VGPRGENQIVVAPGVNASLELADADLDTIATADVLLTNHEVPPAVVRAALDAARRHER
                     LSILNPAPARALPADVLRLGPILTPNEHELVVGIGRDTAEAALDELVARHAGPVVVTQ
                     GPAGALLARGDERTRFDGFVAPSVVDTTGAGDAFNGVLAAWLAFGASLEDAIVAANAA
                     GALSVAAAGARGGLPSRAEIEELVGLR"
     gene            9361..11583
                     /locus_tag="VFV59_09220"
     CDS             9361..11583
                     /locus_tag="VFV59_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEU5326046.1"
                     /translation="MRWGAGALTVVLTALVLGGCLPAHAAGTLAPTLAMVDVAGGTIA
                     QQNGIPVPSFEWQARPRIDLDGPWRVEREPMDQALTMRDRSDALPTIEEEAAGRQAPG
                     YDDSAWATLAVPGTTNPPPDGDETGAWYRRTFELPLEWTGRAVTLRFASANYVADVWV
                     NGTWVGYHEGGSTPFAFEVGHLLRAGAQNLVAVRVHTVGLGTRADIVPWGLIDWWNYG
                     GLTGSVWLEATPPASTVRADVVPHLDAVEVDVVVAHTAALVGTRTSPGDVVTPGGNAT
                     VTATILPATVRPSNLLSPDPRDLLAPSASVQLETSQEVQVPSTGEATVVPLSLSFGDA
                     DLWSPQRPALYVLRVELRWNDRPLDAREARQGDVFLTTFGIRHLAVDESRVLLNGTPI
                     FLRGVGVHDESLAFGPDGALIGGSPHETPQQVRDMLGDASRIGANFLRAGHQPASPTL
                     LTLADRLGFAVWEEIPLYHATPYVFERVMARGIPQQMLREMALRDMNHPSVLFHGFAN
                     ESTGQDERQAALAELHDIDRAIDGTRLTGQAAYGWQPDDPTHAPLDVAGFTFYHGVFY
                     GADAADGTRRALGIAHETHPDKPIMVLEFGRWADTLADQAAQHRIFEETYPMLQRFRA
                     DAPGGFLAGATWWTLRDFATQIAGIEVEDFGLITPTGELRPAGQAAADAFHAPAGEGA
                     QQAIEPELERPRSSAAAGLGEWRLAAYLGYATLTSLVMLGAVTFALTRRGGRALGRRR
                     "
     gene            11580..12650
                     /locus_tag="VFV59_09225"
     CDS             11580..12650
                     /locus_tag="VFV59_09225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5326047.1"
                     /translation="MRYVRGRLAGVSVGASFSAGLWVGAAIGIAIGAVLGALVSWFAG
                     TVLGWQRDLAFTLGVARTLLPLGDQVAALRWLSNTWYLVIPITALIVAVFVGVLGGFI
                     GALLAAAYNRSPRHASVVVELPDEGQATAAGILPEMSTRLPPPAAVVFDLDGTLVDTV
                     DARIEAWRAVFAEEGIESTREELAPLIGIDGRRLAREVAGDRGRTLSDADAERIDHRA
                     GELFDERNREPRALPGAAGVLARLDVAGVTWAIATSSREEQVHASVAALGLDEPPRVV
                     NGSRVAHAKPAPDLLLLAASELGVDPTATWYVGDSTWDMQAARAAGMMAVGVLAGAAV
                     GPEALEEAGATLVIETLDDLVL"
     gene            12653..>13236
                     /locus_tag="VFV59_09230"
     CDS             12653..>13236
                     /locus_tag="VFV59_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5326048.1"
                     /translation="MPLEEYRRKRDFGATPEPAPSDVLERSGRFTIQRHRATALHYDT
                     RLEVGGVLVSWAVPKGPTLDPKARRRAVHVENHPIEYADFEGGITHGEYGGGDVIVWD
                     RGVWQPVEDDPARAIEDGTLHFDLDGEKLAGRFVLVRTRRDTGGKEQWFLLHKQDEHA
                     QAGWDAEDHPQSVKSGRTNDEVAAAPDALWHGDRP"
BASE COUNT         1836 a         4760 c         4675 g         1965 t
ORIGIN      
        1 cgcgctctga gatgacgcgc ttgacggtac cggtggtcat gagatgaagc ccttctctcc
       61 tcgaactgtc gtgcggcgct cggccggttc gagaagaacc tcatcgacca ggcgaagact
      121 gccgagatgc ggcaggaccg ccagcgtacc acgctctgcc cagcggaacc tgtgcagggc
      181 ctggggccag ggtcgtgctg gcacgaccga tctgctagca tcgtcggatc gcgaggcacc
      241 agcagccgcc gagaggctca cgcaggtggc aaccgcgaca tcggtcgggc ggctagctca
      301 gctggttaga gcacttgctt gacatgcaag aggtcactgg ttcgagtcca gtgtcgccca
      361 ccatctccca cggccctgct cgcctacacc tcttgcccgg aggatcgtgc ttccggaagt
      421 agggtcgggc tacgattaca ccacttgcgc agccgcgcca ccgttccgat cggcgccacc
      481 gtgccgcctg cccatcacgc tgttgaccag tatgaagcga cgagagcgct cagcacggcg
      541 acggagcgaa gcaggcggcg cagcggccag tcctccgatt gttgatcggg cgctcgcatt
      601 agcgcggcac tcactgccca tctgacaccg gcgcgggtcg tttggctccg gccacgcggt
      661 ccttacgctt gcgccgcgaa cctatcgttc gtccacggtg tcaggtggac atggggcacg
      721 acctctgagc gcgctcgtcg agcggtcgat gcgaggcgac acggcggcct tcgaggagtt
      781 gaccgtggcg tactcgcctg agatgttccg cctggcggcc gcgatggtcg ggcccgacga
      841 cgccgcggac atggtgcagg aggcattcgt atcggcatgg cgcgagctgc ctcggctgcg
      901 cgacgcggat cgatggtcgt cgtggctacg cagcatcctc atgaaccgat gccgcaacgc
      961 cctccgcacc cggacgcgcc ggccacagct tgtgctgctg gacccggacg gccacgcaac
     1021 gcctcgtcag gtggcgcatg agccgatgcg ggatgtcgac cgccgatggg ctatgcatga
     1081 tgcgctcggt gcgctgcgtc ccgaggaccg cgcggtgatc gtgctgcact acctcgccga
     1141 cctgccgctg cgccaggttg caagcgtgct cgggctgcga ctcggcaccg tgaagtctcg
     1201 gctcaacgcc ggcctgcgcg ccctgcgcgc ggccggtcat gatgaggagc tggcatgacg
     1261 gcgctgcccc ccgacgccca gcgctatcta gggcgaatgc gatcgctcga gcccccggtc
     1321 gagctggtcg acagcatcat ggccgaggtg gaggcgaccc cacaggttcg atccgggtcg
     1381 gacctgcgcg ccatgtccac gttcgttctg gccgcggccg cggtacttct cgccatcgcg
     1441 gtgctgttgc gaattggagc gcaggacgtt ggacctgcgc cgacgcccgt gccactcgac
     1501 cagctgccgt cggccgggtc ggtccaggcc cggatctcga tcgaggccag cgatgtgccg
     1561 gccgccttcg gccatgaatt cctgtggctg acgaatgcca cgaccggcga gctcgtgcgc
     1621 atggacccgg ccaacggcag cattgccagc ccgctccaag tcacacgcgc cggtggcgag
     1681 gtggcgatcg cgatcagcga gagctcggtc tgggtcgcgg atcggcgcga tgcgaccgtg
     1741 gtggaactgg atccggcgac gctggaggag cggcgccgca tcccggtaac cgccactgtc
     1801 agtgccgtgg ctaccctcgg cgccgacctc tggctgctcg acaccggggc gggacagctg
     1861 gtccggttcg atacggccga cgccggcgcg gcgctgaccg tcccggtcga cggcccttcg
     1921 gcgctgctca tccacgatgg cgcagtctgg gtcgcggacg agctcggtga gctggttcgc
     1981 atcgatcccg cctccggcga cgagaccggg cgtgtcgatc tcggcttcgc cgtcatgcgc
     2041 ctcgttcccg acggcggctc gctacttgcc gtcggcgggg cggccgatct cgtcgcccgg
     2101 atcgacgtcg gctccatgcg ggtgtcggcc cgcggcgtcc gcgccccggc gatcgccgtc
     2161 gagggagggc gggtctgggc cgtgacggca tccggctacc tggtcagcct ggatccggcg
     2221 accctgcagc cgctcgcggc aagcgccctc gagctggacg catccgggac gctggcgttc
     2281 ggcggcggct tgctctggac gacaggcgtc gacgccgccg gcgacgcgta cttgctgaag
     2341 gtggtcccgc agccgtgatt ggcgacatgc gaacctttcc ggctgccgcg gtgtcagctg
     2401 agcagcggcg gggcgcacat gcccagccga aagcctgaga ggagccttcc gtgcgcccga
     2461 gccacgcgat cgttctggcc ttcctgatct ccggctgctc ggccgcggcc gatgcgaccg
     2521 ccgcggcatc tgcgtccgcc tccatgacgg tatcggccca gtcatcgccg tcgccctcgg
     2581 ctggtgcgac cgagccggat tggtcggcac tacccaggct cgacgagagc gtgacagcta
     2641 gggtgcccat aaccggcggc tggtccgtcc tcatgggtgc tgcgccagtc gcgtacggga
     2701 gcctctggat ctccaatggc agcgaggctg gtgagccgcc tcggctgcgc cggctcgacc
     2761 gtgagaccat ggaagtcacg gccaccatcg atctcggcgg tgcggcggac gtgtttccgc
     2821 ccgacgccta cggcgctttt tactcggcgc atgggatctg ggtcccgctc atgtctcaga
     2881 aagcggtcgt tctggtcgat ccgaccacga acagcgtctc ggggcgaatc gaggttgacg
     2941 ctacgccgta cggcctgctt gaggacggcg acgacctgtg gatcaccgac ttcggcaact
     3001 cggaagtgct gcgcgtggac attccgaccg gcgaggaacg gctccgcgtc agcatcccgg
     3061 agcccaccgg gatggcgagc ggtcccgaag gcctttgggt gattgagcac gaaaccggct
     3121 tcgtcacgcg gctggacccg gtcaccggcg aggagctggc tcgcgtgcag gtcggtggac
     3181 ggccgggcat cgtcctcggt cttggcagcg tctgggcgag cagcagcgac gaaaagacgg
     3241 tgtcgcgtat cgacccggcg acaaacgccg tgatcgcgac aatcgtcctg ccgtcccacc
     3301 cgagcggcgc agccatcgcg ggcggctcac tgtggctcac tgtgggtccg cagcgcggcg
     3361 cctgcgagcg gacaagctac ctggtgcgaa tcgatcccgc cacgaatgaa gtcgacggca
     3421 tcctagatgt gccatgcgcc ggcagccccg tctcagacgg tgcgcgcgtg tgggtcaaca
     3481 gcggcgagga ggacgcggtc tggatcctga gcgttgaggc cggcgggcgg ccatgaagct
     3541 gagcccacct tggcagggct aacgcttgcg gcgtccgcat ccgctggccc actgtagccg
     3601 cgccggaggc cgcagtgtct cagtcccctg gtgttggcct ggctccagtg ccaccacctc
     3661 cgcttcctga gctagaccgg accgccacga gggcggtcag tgtgttcggt tgcacccggg
     3721 cacgtaggat ggcagccgtg ctgatcatcc tcccgccctc cgagaccaag cggccggcgc
     3781 cggacgaggg atcgccgctg gacctggacg cgctgtcgtt cccggagctc aagccgatgc
     3841 gcgagcgcgt gatcttggct ctgagcgcca ccagccaggc tcccgatgcc ctccggcgcc
     3901 tgttcgtcgg atcgaccctg gcgcccgagg tcgcccgcaa caagctcatg cgtcatctgc
     3961 cgacgaggcg cgccgtggac acgtactcgg ggccgctgta cgtcggcttg gatgcgggat
     4021 cgtggtcgcc ggaggtcgag gcacgggcgg cgcatcacgt cgtcatcgtg tccgcgctgt
     4081 ggggagcgct gcgtccggcg gacctcattc ctccgtaccg cctccacgtc tgcgcgcggc
     4141 tggtgggcat ggaccgcctg gagccggcct ggcgcccgct cctggagccg atcttgaccg
     4201 atgccgccgg acgctcagga ccggtcctcg atctgcggtc gcgcctctac caggccgtgg
     4261 gccgccccac cggcctggac gaccagaccg tcacgctgcg cgttcgcccg gctccgggtg
     4321 gtccggcgca catcggcgac gtcatcgcga agcggatgcg cggtgaggcg gccgcccacc
     4381 tcctcgcgtc ggcaacccta ccgagcgacc cgctggacat cgccgacctg ctggcggagc
     4441 gatggtcagt cgaggtcgat ccgccgagcg tcctcgaccg cagctggacc gtgaccctcc
     4501 gggccgccgc ctgaccgacg gggctgcagg cgcagccggt acaccgggat gcgcgcctcg
     4561 aaccccttga gctccctcga tccgatcgcc tcgagcctgt cggcatcgac gtcgtcggcg
     4621 actgatccgc tcacgatcac ctccccaggc cccgcgatgc ccgagagccg tgcggcgagg
     4681 ttgacggttc gcccgaaata gtcgccgtcc cgttcgacca ccggaccggc ggagacgccg
     4741 atgtgcagcg gaggcagtcc gcgatcgccc gccctgccca tgaggtcggc cgacgccgcc
     4801 acggccgcgc cggcatcgcc gaagacgagc atgaccccat cgccgagctg cttgacgagg
     4861 cgaccgcgat gccgagcggc caggccggtg gcgacctccg ccagcgtggc ggcgtggcgc
     4921 gcggcctggg catctccgcc ctcctcggtc agccgcgtgt acccggtgag atcggcgaag
     4981 acgacgccgc gcgcgtcgtg ggcatgggtc ggtcggacgc ccttgtgctc gaggacccgt
     5041 tccatcgact cgacgttgag cgcgttgagg gcgtgctcca tgtggcgtcg cagcagccag
     5101 agcatcgtcg gctcgaaggc ctcggcgacc cgtgcgcccg gctcgaacag gcgcgtgcgc
     5161 agctcatcgg cccgcatctc cgcgcgttcc tcgatggaca ggccgatggc ctccatgaac
     5221 aggttgaccc acccctcggt tgcccgacgg gcggcgtcgc cgacgaggcg ggcggcgcgg
     5281 tcgcgagcat cggcagccat gccctccccg ccccaggccg tgacgaatcg ggtgatggcc
     5341 gtgacgtccg cctcgcgcag ctcatgatcc gcctcgggga acgggagtcc cagggcgacg
     5401 aacagatcgg cgatcgtggt cggatcgatg cgcaggtcgc ccgcgacgtc ggcgacggtg
     5461 cgacccgacg ccacgctgcc ctccgggtag atccgctcgc tgtaggcgaa ggagacgcgc
     5521 ccctcgcgaa gggcaccgcc caggtcgtcg agggagatgc cgccccgctc gtaggccccc
     5581 gcaatctcga cccgctgcac gtccgcgggc cggaaccgac cggcggcgtc cggcgcgatg
     5641 accccgatct cgacgaggcg ctcgatgcgc gcgaccggga cgccggcccg aagcgcgagg
     5701 tcgtcgagcg tcaggtccat cggcgccatt gtccgtgacg gccccgtccg cgccaagacg
     5761 gtgaccccgg cggccgcgca tgacgttcgg cccttgctcg gcatgtggcg cggtccgagc
     5821 gtacgggttc tcaggtgggg gaggcgtgcc ccttcctggt ggtggatcgc catcgccccg
     5881 gctccgcagc ggagcacgcg ccggcgatgc gagaggcggg acccgcccgc gcgggtgtcg
     5941 acctgcagac gaggggagag tctgagatgt cccaacgacg gacccggttc atgttgccgt
     6001 tggtcgcagc catggtgttc acgatggtgc tggtgccatc ggcctcggct gagacgctgc
     6061 tcgaccagag ccaggagcag gcgaccgcca gctcctcgta catcccgaac gacgaggcga
     6121 acgccggcct ggcgcagacg ttcaaggccg gcctggccgg catcctccac tccatcgacc
     6181 tgaagctcta caaggtcaac acgccgaccg cggggctcaa ggtcgagctg cacaccggac
     6241 caccgaccgg cgccgtgctg gcgacgagcg acgaggtcgg cgcggacaag gtcggcaccg
     6301 atccggccgg agcctggatc tcgttccggt tcgcgacacc ggcgacgctg gtggccggca
     6361 ccgagtactc gctggtcctc gcgcccgcgg cagccgatac gaactcggcg gacccgacct
     6421 acgtgtggtt ccgcagtggt gcggacgcct atgccggagg gacggcgtgg gcggcctcga
     6481 cgtcggccgc gccgatcccg tggctgcagc tgagccccgc ggtcgacttc gcgttccaca
     6541 cctacgtcac gacggcggcc ccgacctgtg acgtggcgct ggccctggtc gacgcgaccg
     6601 acctggtcga cgacgagctg tccgtcgtgg ccggtcagaa gttcgaggcc tggggcttcg
     6661 actacccgcc gctcacggcc gtgacggtca ccctcacgcc gacgagcgga acggcgatca
     6721 cgcaggccgt cagcacggac gcctacggtg agttcgcggt cgagatgccg gccctcgacg
     6781 cggccaaggt cggcgcctgg acggcaaccg cactgccggc gaccgggtgt ccctcgaccg
     6841 cggacgccgt gacgcttgtg gtccgtgcgg cccggaccgt ggaccgcatc ttcggagccg
     6901 accgcttcga gacggcgaag aagatctccg cggcctggtt cgccccggcg accaccgacg
     6961 cggcgttcat cgcgaccggc atgcgcttcc ccgatgccct ggcgggtgga cccgcagccg
     7021 cctgggaggg cgcaccgatc ctgctcagca cgcagaccgc gctcccgacc ccgacacgcg
     7081 atgagctgct gcggctcaag ccggccacga tctacgtgct cggcggaacc ggcgccgtca
     7141 gcgccggggt gcagacgcag ctcgccgcgc tcacgaggga cggcaaggcg acgtcggtcg
     7201 tccggatcgg tggtgctgac cgatacgcca cggcggcgct gatcagccag acgttcttcc
     7261 ccaaggacgc gccggcggtg atgatcgcga ccggacgcaa ctatccggat gcccttgccg
     7321 gcgccgcggc cggtgcccgg tacgggatcc cgatcctgct ggtgaccggg gattcgatcc
     7381 ccgccgcgac ggcagccgag ctgatccgcc tcaacccgga cgagatctac gtcctgggcc
     7441 agacgggcgc ggtgacggca ggggtgcaga cgcagctcgc cgcctacacg tcgagcaaga
     7501 cgcccgcatc ggtcattcgg ctgggcggtg cgacccggta tgacaccgcg gtcgccatca
     7561 gccagcactt cttcccggcc ggcctgtcgc ccgtcttcgt ggcgaccggc gtgaacttcc
     7621 cggatgcgct ggcggcgggc ccgctgggcc gcgccgtgct gctcgtgccc gggacgtcgg
     7681 tgcccatcaa catcgcgaac gaggtccgac gcatcacgcc gaccgacgtg cacctgctcg
     7741 gcggtccgct ggtcgtcagc gacggcgtcg cgacgaccct gaaggcggtt ctcccgtagc
     7801 ggcgaacgct gcggcccggc accctgccga gccgcagcgc atcctgatca gtcggcggcg
     7861 ccgacctgct ggccgagctt cttcagctcg cccatcttcg cgcccagctg ctcgagctcc
     7921 tcgttggaga agacctggcg ggcggtcttg aacatgtcgc cctcctcctc ctcgatgtgg
     7981 tgctcgatgt tctccttcat gacggtgaac ttcgcggccc atcgctcgtc ggtgacgccg
     8041 gtcgcctcga gctcgcccat cacctcgtcg acgacgtggt gctcctcgaa gccctcgagc
     8101 acgatctcct tggccttcgg gtgctgcttg agcgccgggt agaagatctc ttcctcgatg
     8161 cgctcgtgga tgagcatctc ctccttgatc gtgtcgaaca gctgggtgcg ggtcttctcg
     8221 gcccgctcgg tcgtctcctc gccctcggcg agcagcttct tgatccggtc gtggtcgtcc
     8281 ttgagcagcg cgatggcgtc catgcatcct cctcgagtcg ggctcggtgc ggcgacgtct
     8341 gtcgttcgcc gatgcctccg gaaaggcacg tggcgtgccg gttccggcgc atcatcagcg
     8401 caacccgacg agctcctcga tttcggctcg actgggcagc ccgccacgag cgcccgcggc
     8461 ggcgacggac agtgcaccgg ctgcgttggc ggccacgatc gcgtcctcga gcgatgcccc
     8521 gaaggccagc caggcggcga gcacgccgtt gaacgcgtcg cccgcgccgg tcgtgtcgac
     8581 gaccgacggg gcgacgaagc cgtcgaaccg cgtccgctcg tctccacgcg ccagcagcgc
     8641 cccagccgga ccctgcgtca ccaccaccgg tcccgcgtga cgggcgacga gctcgtccag
     8701 cgccgcctcg gcggtgtcac gcccgatgcc gaccacgagc tcgtgctcgt tcggcgtcag
     8761 gatcgggccc aggcgcagca cgtcggccgg gagggcgcgg gccggcgcgg ggttgaggat
     8821 cgagagccgt tcgtggcgtc gggcggcgtc cagcgccgcg cgcacgaccg ccggcggcac
     8881 ctcgtggttg gtgaggagca cgtcggccgt cgcaatcgtg tccaagtccg catccgccag
     8941 ctcgaggctg gcgtttacgc cgggggcgac cacgatctgg ttctccccgc gcgggccgac
     9001 ggcgatcagt gccacgccgg tcggatcggt ggcgtgacgt cgaacccggg acacgtcgac
     9061 gccctcggca accagggcat cgagagcgcg atcaccgttg gcatccgtcc cgaccgcgcc
     9121 gatgaacgcc acggacgcgc cggcccgcgc cgcggcgacc gcctggttcg cgcccttgcc
     9181 cccgtcgtgc gtctcgaagc gaccgccgag caccgtctcg ccgggtcgcg gaagccgctc
     9241 cgccaccacg accaggtcga cgttgatgga cccgacgacg cagaccgtcc cagccgttgc
     9301 tgccatggtg gtcgagtatg cctcagcgcg tggcacgccg gacgctgcca tcatctgacc
     9361 atgcggtggg gagccggtgc gctgaccgtg gtcctgacgg cgctcgtcct cggcgggtgc
     9421 ctccccgcgc atgccgccgg cacgctcgcg ccgaccctcg ccatggtcga cgtggcgggt
     9481 ggaaccatcg cgcagcagaa cggcatcccc gtgcccagct tcgagtggca ggcgcggccg
     9541 cggatcgacc tcgacgggcc gtggcgggtc gagcgcgagc ccatggacca ggcgctgacc
     9601 atgcgcgatc ggagcgacgc cctgccgacg atcgaggagg aggccgcggg acgccaggcg
     9661 ccggggtacg acgacagcgc atgggccacg ctcgccgtcc ccggaacgac caacccgccc
     9721 cccgatggcg atgagaccgg cgcctggtac cgccgcacct ttgagctgcc gctcgagtgg
     9781 accgggcgcg ccgtgacgct gcggttcgca tcggccaact acgtggccga tgtgtgggtc
     9841 aacggcacct gggtcggcta ccacgagggt ggctccacgc cgttcgcctt cgaggtcggc
     9901 cacctgctcc gggccggggc ccagaacctg gtcgcggtgc gcgtccacac cgtcggtctc
     9961 gggacgcggg ccgacatcgt gccgtggggc ctcatcgact ggtggaacta cggcggcctg
    10021 accggctccg tgtggctgga ggcgacaccg ccggcatcca cggtgcgcgc cgacgtcgtg
    10081 ccgcacctgg acgcggtcga ggtcgacgtc gtggtcgcgc acaccgccgc gctggtgggg
    10141 acccggacct cgccgggcga cgtcgtcacg cccgggggca acgccaccgt cacggcgacc
    10201 atcctgccgg ccaccgtgcg cccctcgaac ctgctctcgc cggatccacg cgacctgctg
    10261 gcgcccagcg cgtcggtcca gctggagacg agccaggagg tccaggtccc gtcgaccggc
    10321 gaggcgaccg tcgtaccgct cagcctgtcg ttcggcgacg ccgacctgtg gagcccgcag
    10381 cgcccggccc tctacgtcct gcgcgtcgag ctgcgatgga acgaccgacc gctggacgcg
    10441 cgcgaggccc ggcaggggga cgtcttcctg acgacgttcg gcatccgcca cctggcggtc
    10501 gacgagtcgc gggtgctcct caacggcacg ccgatcttcc tccggggcgt cggggtgcac
    10561 gacgaatcgc ttgccttcgg ccccgacggc gccctgatcg gcggatcgcc gcacgaaaca
    10621 ccgcagcagg tgcgggacat gctgggcgac gcgtcccgga tcggcgcgaa cttcctgcgc
    10681 gcggggcacc agccggcatc gcccacgctg ctgaccctgg cggatcggct gggattcgcg
    10741 gtctgggagg agatcccgct ctaccacgcc actccctacg tcttcgagcg ggtcatggcg
    10801 cgcggcatcc cgcagcagat gctgcgggag atggccctgc gtgacatgaa ccacccgtcc
    10861 gtcctgttcc acggcttcgc caacgagtcg acgggccagg acgagcgcca ggcggcgctg
    10921 gcggagctgc acgacatcga ccgcgccatc gacggcacgc gcctcacggg acaggccgcg
    10981 tacgggtggc agcccgatga tccgacgcac gcaccgctcg acgtcgccgg cttcacgttc
    11041 taccacggtg tcttctacgg cgcggatgcg gccgacggca cgcgtcgtgc gctcggcatc
    11101 gcgcacgaga cgcaccctga caagccgatc atggtgctcg agttcgggcg ctgggccgat
    11161 acgctggccg atcaggcggc gcagcatcgc atcttcgagg agacctaccc gatgctccaa
    11221 cgcttccgcg ccgatgcgcc cggcggcttc ctggccgggg ccacgtggtg gacgctgcgt
    11281 gacttcgcca cgcagatcgc cgggatcgag gtcgaggact tcggcctcat cacgcccacc
    11341 ggcgagctgc gccccgccgg ccaggcggcc gccgatgcgt tccacgcacc ggcgggcgag
    11401 ggcgcgcagc aggcgatcga gccggagctg gagcgaccgc gcagctccgc ggccgccggt
    11461 ctgggcgagt ggcgcctcgc cgcgtacctg ggatacgcca cgctgacgtc gctcgtgatg
    11521 ctcggggcgg tcacattcgc gctgacccgt cgcggcggtc gcgcgctggg caggcgtcga
    11581 tgagatacgt tcgcgggcgg ctggccggcg tctcggtcgg tgcgtcgttc tcggccggcc
    11641 tgtgggtcgg cgcggccatc ggcattgcca tcggcgccgt gctgggggcg ctcgtctcgt
    11701 ggttcgcggg cacggtcctg ggctggcagc gcgacctcgc cttcacgctg ggcgtggcgc
    11761 ggaccctcct gccgctcggc gaccaggtcg cggccctgcg ctggctgtcg aacacctggt
    11821 acctggtgat cccgatcacc gcgctcatcg tggccgtctt cgtgggcgtg ctcggtggat
    11881 tcatcggcgc gctgctggcc gccgcgtaca accgctcgcc ccgccacgcc tcggtggtgg
    11941 tcgagctgcc tgacgaagga caggcgacgg cggccggtat cctgcccgag atgtccacgc
    12001 gcctcccgcc gcctgccgcc gtcgtgttcg acctggacgg aacgctggtc gacacggtcg
    12061 acgcgcgcat cgaggcctgg cgggcggtct ttgccgagga ggggatcgag agcacgcgcg
    12121 aggagctggc acccctcatc gggatcgacg gccggcgcct ggcccgcgag gtggccggtg
    12181 accgtggccg gacgctcagc gacgccgacg ccgagcggat cgatcatcgt gccggcgagc
    12241 tgttcgacga acgcaaccgg gagccgcgcg cgctccccgg ggcggccggc gtcctggcgc
    12301 gcctggacgt ggccggcgtc acctgggcca tcgccaccag ctcacgggag gagcaggtcc
    12361 atgcgtccgt cgcggcgctc gggctcgacg agccgccccg ggtggtgaac ggcagtcgcg
    12421 tggcgcacgc caagccggcg cccgacctcc tgctgctggc agcgagcgag ctgggggtgg
    12481 atccgaccgc gacctggtac gtcggcgact cgacctggga catgcaggcg gcacgggccg
    12541 ccgggatgat ggcggtcggg gtcctggcgg gcgccgccgt cggccccgag gccctggagg
    12601 aggccggggc gacgctggtc atcgagacgc tggacgacct ggtgctctag gcgtgccgct
    12661 cgaggagtac cggcgcaagc gcgacttcgg cgcgacgccg gagcccgcgc ccagcgatgt
    12721 cctcgagcgc agcggccgct tcaccatcca gcgtcaccgc gcgaccgcgc tgcactacga
    12781 cacgcgcctc gaggtcggcg gcgtgctggt cagctgggcg gtgccgaagg gacccacgct
    12841 ggacccgaag gcccgcaggc gggccgtgca cgtcgagaac cacccgatcg agtacgccga
    12901 cttcgagggc ggcatcacgc acggcgagta cggcggcggc gacgtcatcg tctgggaccg
    12961 cggggtgtgg cagccggtcg aggacgaccc ggcccgcgcg atcgaggacg gcacgctgca
    13021 cttcgacctc gacggcgaga agctcgccgg gcggttcgtc ctggtccgca cccgccgcga
    13081 caccggcggc aaggagcagt ggttcctgct gcacaagcag gacgagcacg cccaggccgg
    13141 ctgggacgcc gaggaccacc cgcagtcggt gaagagcggc cgcaccaacg acgaggtcgc
    13201 ggccgccccc gacgccctct ggcacggcga ccggcc
//
LOCUS       DASTYP010000207         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1328560, whole genome shotgun sequence.
ACCESSION   DASTYP010000207 DASTYP010000000
VERSION     DASTYP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328560"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VFV59_09235"
     CDS             complement(<1..1131)
                     /locus_tag="VFV59_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5326049.1"
                     /translation="MRVLLSTWGSRGDVEPLAALALRLRELGAEVRVCAPPDDEFQAL
                     FDRVGVPVIPLGPSLASLRTRFTVPSPDDAFRLAAELVAARFEVLPVAADGCDVLVAT
                     GLMPAGAPDVAEHLGLRYVLATFHIFGLPSAHFPPAARPGKQSPADETDNRVLWQQDA
                     ERVNALYAEPLNRHRAAIGLPPIDNVRDHVMSGQVLLAADPLLAPWKDLTDLDVVQTG
                     AWILPDERPLPADLARFLDAGAPPVYVGFGSLPMRASPDIARIAIEAVRVHGGRVVLA
                     RGAAGLEPIDDAGDCVVVGEVNQQALFRRVAAVVHHGGAGTTTTAAIAGAPQVIVPQL
                     VDQPHWARRVAELGIGAAHEGPVLTVESLAAAVATALDEETRE"
     gene            complement(1362..1988)
                     /locus_tag="VFV59_09240"
     CDS             complement(1362..1988)
                     /locus_tag="VFV59_09240"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HEU5326050.1"
                     /translation="MSGQARSIVAVTAGLSQPSSTRLLADRLVAATERSLSDRGVEVT
                     TEVVELRDLAQDLVNNLLTGFPSERLRETIERVLAADGLVAVSPIFSASYSGLFKMFF
                     DVVERDRLAGMPVLLAATGGTARHSLALEHALRPLFAYFGAATVPTAVFAAAEDWGQA
                     DRPADIGLRERIERASSELAAAVAARSAPAIRDEFEDPIPFEEMLRGG"
     gene            complement(1985..>2534)
                     /locus_tag="VFV59_09245"
     CDS             complement(1985..>2534)
                     /locus_tag="VFV59_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5,10-methylene tetrahydromethanopterin
                     reductase"
                     /protein_id="HEU5326051.1"
                     /translation="GFFHNNIFWPGFHVRRMVGLYRRRFEHYGHGPADTAIVGLGGQV
                     FMRRNSQDAVREFRPYFDHAPVYGGVSSMEEYADTTPLTVGSPEQVIERTLGFREFAG
                     DYQRQLFLVDHAGLPLKTVLEQIEMLGEIVPVMRREFEAMRPAHVPSDPPTHTSLLAA
                     RKADAAAAAAPAPEPTVAGARR"
BASE COUNT          375 a          900 c          945 g          314 t
ORIGIN      
        1 ctcgcgggtc tcttcgtcca gcgccgttgc caccgccgcg gcgagcgact cgacggtcag
       61 gaccggtcct tcgtgcgcgg ccccgatgcc gagctccgcg acgcggcgtg cccagtgcgg
      121 ctggtccacg agctgcggga cgatcacctg gggtgcgccg gcgatcgcgg ccgtggtcgt
      181 cgtccccgcc ccgccgtggt ggactaccgc cgcaacgcgg cggaagagcg cctgctggtt
      241 gacctcccca acgaccacgc agtcgcccgc gtcgtcgatc ggctcgagtc cggcagcgcc
      301 acgcgccagc acgacccgac cgccgtgcac gcggaccgcc tcgatcgcga tgcgcgcgat
      361 gtcgggactt gcgcgcatcg ggaggctgcc aaagccgacg tagaccggcg gcgcgcccgc
      421 gtcgaggaag cgcgccagat cagccggcaa ggggcgctcg tccggcagga tccacgcccc
      481 ggtctggacg acgtcgaggt ccgtcaggtc cttccacggg gccagcagcg ggtccgcggc
      541 gagcaggacc tggccgctca tgacgtgatc gcggacgttg tcgatcggag gcaggccgat
      601 cgccgcccga tgccggttca gcggctccgc gtagagcgcg ttcacccgtt cggcgtcctg
      661 ctgccacagg acccggttgt cggtctcgtc cgctggggac tgcttgccgg ggcgagccgc
      721 cggcgggaag tgggccgagg gcaggccgaa aatgtggaac gtggcgagca cgtagcgcag
      781 gccgaggtgc tcagcgacgt cgggcgcgcc ggccggcatg aggccggtgg ccacgagcac
      841 gtcgcagccg tcggcggcaa cgggcagcac ctcgaaccga gcggcgacca gctccgcggc
      901 caggcggaag gcatcgtccg gcgacgggac ggtgaaccgc gtgcgcagcg atgccagcga
      961 tggcccgagc gggatgactg gcacgcccac gcgatcgaac agcgcctgga actcgtcgtc
     1021 cggcggagcg cagacgcgga cctcggcacc gagctcgcgg agccgaagcg cgagcgcggc
     1081 gagtggctcc acgtcaccgc gcgatcccca ggtcgacagc agtacccgca cgtcgtgact
     1141 cccgtgccgt ttgcgagacg aagcgggcga ctctagcgca tctcgctccg ggccgagtct
     1201 cgccgcgaga ccgagagccg cgccgcggga accgagggcc gggaccgcgg tgcgccgcgc
     1261 tgcggcagca gatgacccgc ggggcggctc aaccgagggc tgaggtcacg acgcggcggg
     1321 ctccggcagc agatgacccg tcgggcgggt caacgcacct cctacccgcc gcggagcatc
     1381 tcctcgaacg ggatcgggtc ctcgaactcg tcgcggatgg ccggtgccga gcgcgcggcc
     1441 accgccgcgg cgagttccga gctcgcccgc tcgatgcgct cgcgcaggcc gatgtccgcc
     1501 ggccgatccg cctgccccca gtcctcggcc gcggcgaaga ccgccgtcgg cacggtggcg
     1561 gccccgaagt acgcgaagag cggccggaga gcgtgctcga gcgccagcga gtggcgcgcg
     1621 gtgccacccg tggcggcgag cagcacgggc atgcccgcca gccggtcgcg ctcgaccacg
     1681 tcgaagaaca tcttgaacag ccccgaatac gaggcgctga agatcgggct gaccgcgacg
     1741 agcccatcag cggccagcac gcgctcgatc gtctcgcgca gacgctcgct cgggaagccg
     1801 gtgagcaggt tgttgaccag gtcctgcgcc aggtcgcgca gctctacgac ctccgtggtc
     1861 acctcgacgc cgcggtcgct cagcgaacgc tcggtcgcgg ccaccagccg gtcggcgagc
     1921 aggcgggtcg aagacggctg gctcaacccg gccgtcacgg cgacgatcga gcgggcctgg
     1981 ccgctcaccg tcgggctcct gcgacggtcg gctcgggagc gggagcggcg gcggcggccg
     2041 catcggcctt gcgtgcggcc agcaggctgg tgtgggtcgg cgggtcggag ggcacgtgcg
     2101 ccgggcgcat ggcctcgaac tcgcgccgca tgacggggac gatctcgccc agcatctcga
     2161 tctgctccag cacggtcttg agcggaaggc cggcgtggtc gaccaggaag agctggcgct
     2221 ggtagtcgcc ggcgaactcc cggaagccca gggtccgttc gatcacctgc tccgggctgc
     2281 cgacggtcag cggcgtcgta tcggcgtact cctccatcga cgagacgccg ccgtagaccg
     2341 gggcgtggtc gaagtagggc cggaactcgc gcacggcgtc ctgcgagttg cggcgcatga
     2401 acacctggcc gccgagcccg acgatggcgg tatcggccgg tccgtgcccg tagtgctcga
     2461 agcggcgccg atacagcccg accatgcgcc ggacgtggaa cccgggccag aagatgttgt
     2521 tgtggaagaa gccg
//
LOCUS       DASTYP010000208         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1730829, whole genome shotgun sequence.
ACCESSION   DASTYP010000208 DASTYP010000000
VERSION     DASTYP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730829"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..2760
                     /locus_tag="VFV59_09250"
     CDS             19..2760
                     /locus_tag="VFV59_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5326052.1"
                     /translation="MSRSALVAPSFATLAGDSATPEDRTANARTLRIAAAFVVVTVVA
                     AFLPVPAGNESLAAAGIRLVAAIGALLVNAHGWRAATRPEDAAFRSLILRITIVWLAL
                     ETLKLGAVLSGVASIGPLEGLLLGAVVVMAMRAWPLLLRDRLDHAELVAVVLDSAAVF
                     VGLAAVVLFGLATAGIGTLTAAGAELRGILFMGLAGAVGVLYLSIAPVRGIHGWSMVI
                     PGLVLLGGGNLLAAVAPDVGPIPVTVLVSLGVLVTSYGTATWSGVHDPDEGFQRLAAR
                     IRRLLPIGAVAMASVIMVASELRPHEHGGLMALLISATLASVLVVGVIRQTILLNDRD
                     AMTRRAMEAVEREREALRDLQLSEERFRALLKHSSDVFLILATDGTVLYQSPAVQSVL
                     GYAPDERLGRSILELTHPDDIPYVRTTIGELIAQPGGMRTIELRTKHADGSWRILEAS
                     GWNLTDDPSVAGIVVNYRDITERKTLEQQLVHDAFHDALTGLANRALFVDRTQHALAR
                     RPGSAGVAVLIMDVDDFKTINDSLGHSAGDQAMVAVAERLRACLRPEDTVSRVGGDEF
                     AILLEDADEELAASVSERILDTLRRPFSLGGIEVHLSGSIGVAFSNADTRAADELLRN
                     ADVAMYTAKNRGKGRAERFESSMHAAALARLELRTDLERAVERGELRLRYQPIYALRD
                     GALSGFEALLRWRHPTRGEIMPNDFIPLAEETGLIVRIGNWVLEQACRQAVAWSETAG
                     RPIRISVNVSPRQVREPSMAEWVRLALVSTGLAPDQLIVELTESSLMQDDENQLHELK
                     ALGVQLALDDFGTGYSSLSYLARFPIDQLKVDQSFVAQLEHGLDVPPLVRSVVQLARS
                     LKLATVAEGVEEEAQVDLLRSMGVTYGQGYLFARPMDAIAATRLVSLDEPMRCRAAS"
     gene            2809..>4734
                     /locus_tag="VFV59_09255"
     CDS             2809..>4734
                     /locus_tag="VFV59_09255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU5326053.1"
                     /translation="MLYDYAAVTPDSVRAETDAAIADADALVAGAVASAPDPSFDATL
                     LPLELAGARLVEAYGRGAFMGQVHPDEAVRDAGTDAEERLNKWRVAVVFRRDLYEAVR
                     AFAATDAASALEGERARLLEHWLRDFRRAGHELSDEGRDELERLRTRLVEVEVAFQRN
                     INEYRDGIDVTREQLEGLPDDYVERLTNGSTPGTYRVSLDYPELNPFMEQAHDRGLRR
                     TLFLKHWNRAVEVNRPLLAEALELRQRIARLMGVPTWAHHGMELKMAQTPERVRALYD
                     ELAPAMAAKIRDELAVMAERLHADGHEGPIMEWDRRYYGELLRRTEFGVDQNRVSEYL
                     PLERVMDGMFALTGDVFGLDYRSVDDAPVWHDSVRVYEILDRASGEHLAHFYADLFPR
                     EGKFGHAAAFPLVIGHRTADGPYVAPVSAIAANFTPPSADRPALLRHTEVETLFHEFG
                     HVLHMSLTRAEFARFSGAETEWDFVEAPSQIMEHWTWEPSVLGRFARHHATDEPIPPD
                     LVEQMRRARWLNVGLHLGVQAFYGGIDLALHGEPVVPDLDEALRRTYAVTGMPYPDGT
                     FMLTGFGHLLGGYDAGYYGYLWAEVIGDDMFGRFAREGVVSPSVGADYRREILEPNGS
                     RDGDALVEAFLGRPFSNA"
BASE COUNT          648 a         1741 c         1638 g          707 t
ORIGIN      
        1 tccgtacgct tcgaggcgat gagccgatca gcgctggtgg cgccgtcctt cgcgacgctc
       61 gccggggaca gcgcaacgcc cgaggaccgg accgccaacg cgcgcaccct gcgcatcgcg
      121 gcggcgttcg tcgtcgtcac ggtggtggcc gccttcctcc ccgtgccggc cgggaacgag
      181 agcctggcgg ccgccggcat ccgcctggtc gccgccatcg gggcgctgct cgtcaacgcg
      241 cacggctggc gggcggcgac acgccccgag gacgccgcct tccgctcgct gatcttgcgc
      301 atcaccatcg tctggctggc gctcgagacc ctcaagctcg gtgccgtgct gtccggcgtg
      361 gcgagcatcg gcccactcga agggctcctg cttggcgcgg tggtggtgat ggcgatgcgc
      421 gcctggccgc tgctgctgcg cgatcggctc gaccacgccg agctggtggc cgtcgtgctc
      481 gactcggcgg cggtgttcgt gggccttgcc gcggtggtgc tgttcgggct ggccaccgcc
      541 ggcatcggga cgctcaccgc cgccggtgcc gagctgcggg gcatcctgtt catgggcctg
      601 gccggcgccg tcggcgtgct ctacctgtcc atcgccccgg tccgtggtat ccacggctgg
      661 tcgatggtga tccccgggct cgtgctgctc ggcggcggga acctgctcgc ggccgtcgcg
      721 ccggacgtcg gccccatccc cgtcaccgtg ctcgtctccc tcggggtgct ggtcacgtcg
      781 tacggcacgg cgacgtggag cggcgtccac gatccggacg agggattcca gcgccttgcc
      841 gcccggattc ggcggctgct gcccatcggc gcggtcgcca tggcctcggt gatcatggtc
      901 gcgagcgagc tccggcccca cgagcacggc gggctcatgg cgctcctcat cagcgcgacg
      961 ctggcgagcg tcctggtcgt cggcgtcatc cgccagacca tcctgctcaa cgaccgcgac
     1021 gcgatgacgc gcagggcgat ggaggccgtc gagcgcgagc gggaggcgct gcgcgacctc
     1081 cagctcagcg aggagcgttt ccgtgccctg ctgaagcact cctcggacgt gttcctgatc
     1141 ctggcgacgg atgggacggt gctctaccag agccccgcgg tccagtccgt cctcggctac
     1201 gcgccggacg agcgcctggg ccgctcgatc ctcgagctca cccatcccga cgacatcccg
     1261 tacgtgcgca ccaccatcgg ggagctgatc gcgcagcccg gcgggatgcg aacgatcgag
     1321 ctgcggacga agcacgcgga cggctcgtgg cggatcctgg aggcgtcggg ctggaacctg
     1381 accgacgacc cgtcggtcgc cggcatcgtc gtgaactacc gcgacatcac cgagcgcaag
     1441 acgctggagc agcagctggt ccacgacgcg ttccacgacg cgctgacggg cctcgccaac
     1501 cgcgccctct tcgtcgatcg gacccagcac gcgctggcgc ggcgcccggg cagcgccggc
     1561 gtggccgtgc tgatcatgga cgttgacgac ttcaagacca tcaacgacag cctcggccac
     1621 tccgccgggg accaggccat ggtggccgtc gccgagcggc tgcgcgcctg cctgcggccc
     1681 gaggacacgg tgtcccgcgt cggaggcgac gagttcgcca tcctcctcga ggatgccgac
     1741 gaggaactgg ccgcatcggt cagcgagcgc atcctggaca ccctgcgccg gccgttctcc
     1801 ctgggcggca tcgaggtcca cctgtccggc tccatcggcg tggccttctc caacgccgat
     1861 acccgcgccg ccgacgagct gttgcgcaac gccgacgtgg ccatgtacac ggcgaagaac
     1921 cgcggcaagg gtcgcgccga gcggttcgag tcgagcatgc acgccgccgc cctcgcccga
     1981 ctggagctgc gcacggatct cgagcgcgcc gtcgagcgtg gcgagctgcg cctccgctac
     2041 cagccgatct acgccctccg cgacggcgct ctctccggct tcgaggcgct gctgcgctgg
     2101 cggcacccga cccgcggcga gatcatgccg aacgacttca tcccgctggc cgaggagacg
     2161 gggctcatcg tccgcatcgg caactgggtg ctggagcagg catgccgcca ggccgttgcc
     2221 tggagcgaga cggccggacg gcccatccgc atcagcgtca acgtctcgcc acgccaggtg
     2281 cgggagccct ccatggctga gtgggtccgt cttgccctgg tctcgaccgg cctggcgccg
     2341 gaccagctga tcgtcgagct gacggagtcg agcctgatgc aggacgacga gaaccagctg
     2401 cacgagctca aggccctggg cgtgcagctg gcgctggacg acttcggcac cggctacagc
     2461 tcgctcagct acctcgcccg cttccccatc gaccagctca aggtggacca gagcttcgtc
     2521 gcgcaactcg agcacgggct cgatgtcccg ccgttggtcc gctcggtcgt gcagctcgcc
     2581 cgaagcctga agctcgccac ggtggccgag ggcgtggagg aggaggccca ggtcgacctc
     2641 ctgcgcagca tgggcgtcac ctacgggcag ggctacctct tcgcccgacc gatggacgcc
     2701 atcgccgcca cccgcctcgt cagcctggat gagccgatgc gctgccgcgc cgcttcctga
     2761 gaccttcgcg cttcgcgccg gggtgccccc gcgcgtagga tgcgcgcgat gctgtacgac
     2821 tacgccgccg tcaccccgga ctcggttcgc gccgagaccg atgccgcgat cgccgacgcc
     2881 gatgccctcg tcgcgggtgc ggtcgcctcc gcccccgatc cgtcgttcga cgccaccctg
     2941 ctgcctctcg agctcgctgg ggcgcgcctg gtcgaggcct acggacgcgg cgccttcatg
     3001 ggccaggtcc atcccgacga ggcggtccgc gacgcgggga ccgatgccga agagcgcctc
     3061 aacaagtggc gcgtggcggt ggtcttccga cgcgacctgt acgaggcggt ccgcgccttc
     3121 gcggccaccg atgccgcgtc ggcgctcgag ggcgagcgag cgcggctgct ggagcactgg
     3181 ctccgggact tccggcgcgc cggccacgag ctgtccgacg aaggacgcga cgagctcgag
     3241 cgactgcgca cccggctggt ggaggtcgag gtcgcgttcc agcgcaacat caacgagtac
     3301 cgcgacggca tcgacgtgac gcgggagcaa ctcgagggcc tccccgacga ctacgtcgag
     3361 cggctgacga acggctccac gcccgggacg tatcgggtca gcctggatta ccccgagctc
     3421 aacccgttca tggagcaggc gcacgaccgc gggctgcggc gcacgctgtt cctcaagcat
     3481 tggaaccgcg cggtcgaggt caaccgtccg ctcctggccg aggcgctcga gctccggcag
     3541 cggatcgccc ggctgatggg cgtgccgacg tgggcgcacc acggcatgga gctgaagatg
     3601 gcgcagacgc ccgagcgggt gcgtgcgctc tacgacgagc tggcgcccgc gatggcggcg
     3661 aagatccggg acgagctggc cgtgatggcg gagcgcctcc acgccgacgg ccacgagggg
     3721 ccgatcatgg agtgggaccg gcgctactac ggcgagctcc tccggcgcac cgagttcggc
     3781 gtcgaccaga accgggtcag cgagtacctg cccctggagc gcgtcatgga cggcatgttc
     3841 gcgctcaccg gcgacgtctt cggcctggac taccgctcgg tcgacgatgc gccggtctgg
     3901 cacgacagcg tccgcgtcta cgagatcctc gaccgcgcca gcggagagca cctcgcccac
     3961 ttctacgccg acctcttccc gcgcgagggc aagttcggcc atgccgccgc cttccccctc
     4021 gtcatcggtc accggacggc cgacggtcca tacgttgcgc cggtcagcgc catcgcggcg
     4081 aatttcacgc cgccatcggc ggatcggcct gcgctgctcc gccacaccga ggtcgagacg
     4141 ctcttccacg agttcggcca cgtcctgcac atgagcctca cccgcgccga gttcgcgcgc
     4201 ttcagcggcg ccgagaccga gtgggacttc gtggaggcgc cgtcgcagat catggagcac
     4261 tggacctggg agccgtccgt cctcggccgc ttcgcgcggc accacgcgac cgacgagccg
     4321 atcccgcccg acctggtgga gcagatgcgt cgtgcgcggt ggctcaacgt cggcctgcac
     4381 ctgggcgtcc aggcgttcta cggcgggatc gacctggcgc tccacggcga gccggtcgtc
     4441 ccggacctcg acgaggccct gcgtcgcacc tacgcggtca ccgggatgcc ctatccggac
     4501 ggcaccttca tgctcacggg cttcggccac ctgctcggcg gctacgacgc cggctactac
     4561 gggtacctgt gggcggaggt catcggcgac gacatgttcg gccgattcgc ccgcgagggc
     4621 gtcgtctcgc cctcggtcgg cgccgactac cgacgggaga tcctggagcc caacggctcc
     4681 cgtgacggcg acgcgctggt cgaggcgttc ctgggccggc ccttctcgaa cgcg
//
LOCUS       DASTYP010000209         4736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1476428, whole genome shotgun sequence.
ACCESSION   DASTYP010000209 DASTYP010000000
VERSION     DASTYP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476428"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFV59_09260"
     CDS             <1..522
                     /locus_tag="VFV59_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326054.1"
                     /translation="QPTPRWHIDVEAPVAETIAPRAGWQRWSAPDSAWPVTLLPPIEA
                     VQAAKLQSLEASEALDRGLRRALYAESRCISLRHVILEVATEADVVDVAALAAALDDG
                     RARSSVIADWHRAQGDEVRGSAHVFAPDGTNDENPGIGVGWEEDGSPAGRYWIEHDDP
                     RAIDELVRRAAQA"
     gene            553..1026
                     /locus_tag="VFV59_09265"
     CDS             553..1026
                     /locus_tag="VFV59_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5326055.1"
                     /translation="MSVHLPPFETLIDAHAAELHRFLVALVGPTDAEDCLQETYMSAL
                     RAYPRLRHGDNLRAWLYTIAQRKATDTVRRSARRPTRELDGVEVAAPASADVADDGLW
                     HAVQRLPAKQRAAVVHRYVLDLAYAEIGARMGISEEAARQNVSAGLRRLRREVPA"
     gene            1023..1664
                     /locus_tag="VFV59_09270"
     CDS             1023..1664
                     /locus_tag="VFV59_09270"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5326056.1"
                     /translation="MTDELERRLGSAPAPALDVAGLRRRLARRADEEGLLDVAYGTYD
                     SPLGPLTVVVTPRGLVRLAYPGEPVEDELADIAARISPRVLEAPERTDEVRRELDAYF
                     AGHRRGFDVPIDWRLVRGFAGEVLRATARIPFGQVSTYRDIAAEAGSPNAYRAAGNAL
                     GSNPVPIVVPCHRVLHAGGGLGGYTGGLDRKRYLLRLEGVLDDEGTRRPVPAS"
     gene            1696..2079
                     /locus_tag="VFV59_09275"
     CDS             1696..2079
                     /locus_tag="VFV59_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326057.1"
                     /translation="MRALTLALLLVLAACGTDAGASPLPSADGGTGDPVLVIVQGDPG
                     NAGISVPEALGHQATDDIVAVSGALFVAADESVLLCELIAESFPPQCGGARITVEGLD
                     LGTVALESANGVRWAESVTLLGSVE"
     gene            complement(2080..2706)
                     /locus_tag="VFV59_09280"
     CDS             complement(2080..2706)
                     /locus_tag="VFV59_09280"
                     /inference="COORDINATES: protein motif:HMM:NF040123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HEU5326058.1"
                     /translation="MAPRLGWMALISIGLGVFSVVGDAIPVLAAPANAVGPWIVAAYA
                     IGAVTGRARSGAVAGAAGLVVAVVTYYVGVRLVWGEAFVDPLRATAVWGVVAVAVGVP
                     LGLAGGAWAARDARWRVTSVALLGGLLLAEALTRFVEVEGWTGIDLGRTALQVCAYDT
                     LGAILAPLLLLERRQLPSAYAASVAVGLAGAVLLALTMSLIRQSAAGA"
     gene            2786..4324
                     /locus_tag="VFV59_09285"
     CDS             2786..4324
                     /locus_tag="VFV59_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5326059.1"
                     /translation="MTLTKKGESRPAPASWGFGAGDVPWEYAPAPEATDHVRIEPEYG
                     LFIGNEFRPSRPRKTFTVINPATEEPLAKVAHASKADVDRAVSTAREAHRSSWARLPG
                     RERAKYLYRIARILQERSREFAVVESMNSGKPIKESRDVDVPLAAAHFFYYAGWADKL
                     EYAYPNRDPRPLGVAAQIIPWNFPLLMLAWKIAPALAAGNTVVLKPASTTPLTAYLFA
                     DVVRQADLPPGVVNIITGPGEIGMELVKHPDVDKVAFTGSTAVGKMIARAVAGTSKKV
                     TLELGGKAANIVFDDAPLDQAIEGIVNGIYFNQGEVCCAGSRLFVQESVYEPLIDKLK
                     ARLSTLRVGDPLDKNTDVGAINSAGQLAKITELVESGIEEGAEIFQPDCELPDRGFFF
                     RPTLFTGVSQSHRIAQEEIFGPVLSVLTFRTPEEAIEKANNTPYGLSAGVWTEKGSRI
                     FKMVNAMRAGVVWANTFNRFDPTSPFGGYKESGYGREGGIHGLGAYLDLSPNGTNGSG
                     ANGR"
     gene            4321..>4736
                     /locus_tag="VFV59_09290"
     CDS             4321..>4736
                     /locus_tag="VFV59_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5326060.1"
                     /translation="MSPSARTKTATATRAKRASVLDARAAQLPATTDGASPRGERIGV
                     RKTYKLYIGGAFPRTESGRAYEVFGAKGELLANACRGTRKDIREAVRAARKAVPGWAS
                     RTAYNRSQILYRIAELMEGRRDQFIAEVVAAEGVSRA"
BASE COUNT          709 a         1736 c         1697 g          594 t
ORIGIN      
        1 cagccgaccc cgcgctggca catcgacgtc gaggccccgg tggcggagac gattgcgccg
       61 cgagcgggct ggcagcggtg gtcggcgccc gactcggcct ggccggtgac gctcctgccg
      121 ccgatcgagg cggtccaggc ggcgaagctg cagtcgctcg aggcgtccga ggcgctcgat
      181 cgaggtctcc gacgcgcgct gtatgccgag agccggtgca tcagcctgcg ccacgtcatc
      241 ctggaggtcg cgaccgaggc cgatgtcgtc gacgtggccg ccctggccgc cgcgctcgac
      301 gacggaaggg cgcgctcgtc cgtgatcgcc gactggcacc gcgcacaggg cgatgaggtc
      361 cgcggctccg cccacgtctt cgcgccggac gggaccaacg acgagaaccc cggcatcggc
      421 gtcggctggg aggaagacgg cagcccggcc ggacgctact ggatcgagca cgacgatcct
      481 cgggcgatcg acgagctcgt ccgacgtgcc gcccaggcct gatctcacga acccgggtgg
      541 cgtgacgttc gaatgagcgt gcacctgccg ccgttcgaga cgctcatcga cgcccacgcc
      601 gcggagctgc atcggttcct cgtcgcgctg gtcgggccga ccgacgccga ggactgcctc
      661 caggagacct acatgtccgc gctccgcgcc tacccccgac tgcgacacgg cgacaacctc
      721 cgagcctggc tctacaccat cgcccagcga aaggcgaccg atacggtccg gcgctcggcg
      781 cgccgtccga cgcgtgagct ggacggcgtg gaggtggccg ccccggcgtc cgccgacgtg
      841 gccgatgacg gtctgtggca tgcggtgcaa cgcctccccg ccaagcagcg ggcggcggtc
      901 gtgcaccgct acgtgctgga ccttgcctac gccgagatcg gcgcgcggat gggcatcagt
      961 gaggaggccg cacgccagaa cgtgagcgcc ggtctgcgac ggctgcgacg ggaggtgccg
     1021 gcatgaccga cgagctcgag cggcgcctgg gcagcgcccc cgctcccgcc ctggacgtcg
     1081 ccgggctgcg gcgacggctg gcccgtcgcg ccgacgagga gggcctgctg gacgtggcgt
     1141 acggcacgta cgactcgccg ctcgggccgc tgaccgtggt cgtcacccca cgcggcctgg
     1201 tccggctggc gtacccgggc gagccggtcg aggacgagct ggcggacatc gcggcccgca
     1261 tctcgccgcg cgtcctggag gcgccggagc ggaccgacga ggtccggcgc gagctcgacg
     1321 cgtacttcgc cggccaccgc cgcggcttcg acgtgccgat cgactggcgc ctggttcgcg
     1381 gcttcgccgg cgaggtgctg cgcgccaccg cgcggatccc gttcggccag gtctcgacct
     1441 accgcgacat cgcggcggag gccggctcgc cgaacgccta ccgcgcagcg gggaacgcgt
     1501 tgggctccaa ccccgtgccg atcgtggtgc cgtgccaccg cgtcctgcat gccggtggcg
     1561 gcctgggtgg gtacaccggc ggcctggacc gcaagcggta cctgctgcgc ctggagggcg
     1621 tgctggacga cgagggaacg cggcgccccg tcccggcgtc ctgacggcga cacccacccg
     1681 cccgaggttc ccccgatgcg cgcgctgacg ctcgccctcc tcctcgtcct ggccgcctgc
     1741 ggcaccgatg ccggcgcctc gccgctcccg agcgcggacg gcggaaccgg tgacccggtc
     1801 ctcgtcatcg tccagggcga tccaggcaac gccggcatca gcgtgcccga ggcgctcggg
     1861 caccaggcga ccgatgacat cgtcgccgtg agcggcgcgc tgttcgtcgc cgccgacgaa
     1921 tcggtgctgc tgtgcgagct gatcgcggag tcgttcccgc cccagtgcgg tggtgcgagg
     1981 atcaccgtcg agggcctcga tctcggcacg gtggcgctcg agtcggcgaa cggcgtgcgc
     2041 tgggccgaat cggtcacgct gctgggatcc gtcgagtagt caggcccccg cggccgattg
     2101 ccggatcagc gacatcgtca gggccagcag gacggcgccg gccaggccga cggcgacgct
     2161 cgccgcgtac gccgacggga gctgacggcg ctcgagcagc aggagcgggg cgaggatcgc
     2221 gccgagcgtg tcgtacgcac agacctggag cgccgtccgg ccgaggtcga tgccggtcca
     2281 cccctccacc tcgacgaagc gcgtcaacgc ctccgccagc agcagcccgc ccagcaacgc
     2341 aacgctcgtg acgcgccagc gggcgtcccg cgcggcccag gcgccgccgg cgagcccgag
     2401 cgggacgccc accgcgaccg ccaccacgcc ccagaccgcg gtggcgcgga gcggatcgac
     2461 gaaggcctcg ccccagacga gccggacgcc aacgtagtag gtcacgaccg ccacgaccag
     2521 cccggccgcg cccgccaccg ccccggaccg cgctcgcccg gtgacggcgc cgatggcgta
     2581 tgccgcgacg atccacggcc cgacggcatt cgccggcgcg gcgagcaccg ggatggcgtc
     2641 gcccacgacg ctgaagaccc ccaacccgat gctgatgagg gccatccacc cgagccgcgg
     2701 cgccatcggg gcgatcgtac cggctgcgga cgccactgac gcctgggcgg tgcgcctccc
     2761 atccgggtgc gataatcggc cggtcatgac gctcaccaag aagggcgaga gccgccccgc
     2821 tcccgcctcg tggggcttcg gcgccggcga cgtgccctgg gagtacgcgc ccgcgcccga
     2881 ggcgaccgac cacgtccgca tcgagcccga gtacggcctc ttcatcggca acgagttccg
     2941 gccgtcgagg ccgcgcaaga cgttcaccgt catcaacccg gcaaccgagg agccgctggc
     3001 caaggtcgct cacgcgtcga aggcggacgt ggaccgggcg gtgagcacgg cgcgcgaggc
     3061 gcatcggtcc agctgggcga ggctgccggg ccgtgagcgc gccaagtacc tgtaccggat
     3121 cgcgcggatc ctccaggagc gcagccgcga gttcgccgtg gtcgagagca tgaactccgg
     3181 caagccgatc aaggagtcgc gcgacgtgga cgtgccgctg gcggcggcgc acttcttcta
     3241 ctacgcgggc tgggccgaca agctcgagta cgcctacccg aaccgcgatc cgcggccgct
     3301 gggcgtcgcc gcgcagatca tcccgtggaa cttcccgctg ctgatgctgg cctggaagat
     3361 cgcgcccgcg ctggcggcgg ggaacacggt cgtcctgaag ccggcgtcga cgacgccgct
     3421 gacggcctac ctgtttgcgg acgtggtgcg gcaggccgac ctcccgccgg gcgtcgtcaa
     3481 catcatcacc ggcccgggcg agatcgggat ggagctggtg aagcaccccg acgtcgacaa
     3541 ggtcgccttc accggctcga ccgcggtcgg gaagatgatc gcgcgtgccg tggccgggac
     3601 gagcaagaag gtcacgctgg agctgggcgg caaggcggca aacatcgtct tcgacgacgc
     3661 cccgctcgac caggcgatcg agggcatcgt caacggcatc tacttcaacc agggcgaggt
     3721 ctgctgcgcc ggctcgcgcc tcttcgtgca ggagagcgtc tacgagccgc tgatcgacaa
     3781 gctcaaggcc cgcctctcga cgctccgcgt cggcgacccg ctcgacaaga acaccgatgt
     3841 cggcgccatc aactcggccg gccagctggc caagatcacg gagctggtcg agtcggggat
     3901 cgaggagggg gcagagatct tccagcccga ctgcgagctg ccggaccgcg gcttcttctt
     3961 ccggccgacg ctcttcaccg gcgtcagcca gtcacatcgg atcgcgcagg aggagatctt
     4021 cggcccggtg ctgtcggtcc tcaccttccg gacgcctgag gaggcgatcg agaaggcgaa
     4081 caacacgccg tacggcctgt cggctggcgt gtggacggag aagggcagcc gcatcttcaa
     4141 gatggtcaac gccatgcgcg cgggcgtcgt gtgggccaac accttcaacc gcttcgaccc
     4201 gacctcgccc ttcggcggct acaaggagtc cggctacggc cgcgagggcg gcatccacgg
     4261 cctcggcgcc tacctggacc tgtcgccgaa tggcacgaac ggcagcggag cgaacggccg
     4321 atgagcccgt ccgcacgcac caagacggcg acggcgacgc gcgcgaagcg cgcatcggtc
     4381 ctggacgccc gtgcagcaca gctcccggcg acgaccgacg gcgcgagccc gcgcggcgag
     4441 cgcatcgggg tgcgcaagac gtacaagctc tacatcggcg gcgcattccc gcgcaccgag
     4501 tccggccgcg cctacgaggt cttcggcgcc aagggcgagc tgctggcgaa cgcgtgccgc
     4561 gggacccgca aggacatccg cgaggcggtg cgtgccgccc ggaaggccgt tccgggctgg
     4621 gcctcgcgca ccgcgtacaa ccgctcgcag atcctctacc gcatcgccga gctgatggag
     4681 gggcgccggg accagttcat cgccgaggtc gtggcggccg agggcgtgtc gcgagc
//
LOCUS       DASTYP010000210         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1658737, whole genome shotgun sequence.
ACCESSION   DASTYP010000210 DASTYP010000000
VERSION     DASTYP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658737"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFV59_09295"
     CDS             <1..294
                     /locus_tag="VFV59_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5326061.1"
                     /translation="GDQMVFVIQSIILLFSGVYYETTALPDWMQFISQFSPGTYVLDG
                     VRAGLIYNKPVTELWNDVWPLLLMGLILIPVGVRVFGMAERYAKRTGKLKRVG"
     gene            321..1337
                     /locus_tag="VFV59_09300"
     CDS             321..1337
                     /locus_tag="VFV59_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5326062.1"
                     /translation="MQTDTAAHAATLPGLVVRPFAGEADLAEIARIQNAEWEADGVRG
                     RTTPEELAAWLSHPSEQFDARRDVSLTEVDGRVVAVTQVDWIDATDGVREYRSRCWLD
                     PVYRRRGIGTMLLLRSEQRRRQMAAGHDTDRPKVLGVGTAETNLGGNALFRRFGYEPV
                     RWFFDMERAIDGGLPEIPALPAGIEVRPVGREHARQVWEADHDAFRDHWGGADASDAS
                     FQRWLDSPEFDPSLQVVAWDGDEIAGAVLNAIYPEENEALGVRRGWLDSVFTRRAWRR
                     RGLARALIVRSLHLLRERGMEGAVLGVDADNPSGALGLYESVGFAATERFVAYRRPLE
                     VPSP"
     gene            1334..2335
                     /locus_tag="VFV59_09305"
     CDS             1334..2335
                     /locus_tag="VFV59_09305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5326063.1"
                     /translation="MSVVLTAAPAIPGLRFRPYAGPQDHPEMHRVHQAVAAANGIEEV
                     ISLEQFDLSYANHVNCDHDRDIVLAEVDGNLVAYSRVFWSELLEGGRSYENFGFVHPE
                     WRRRGIGTAMHRHNEARLREIAGGHAGVGPKWLSSEAVDGDPGAAALLRADGYEPVRW
                     FFEMVAPSLEDVPSVGVPDGVEVRPVTREQYRRIWDAMDEAFRDHWGATPGTEEEWRT
                     FEGDPDNADPRYWRVAWDGDEVAGVVVTVPQTEANARHQRRRVYVASVSVRRPWRRRG
                     LARALLAGSLIGAREDGFTSASLGVDAENPTGALALYESLGFERHRSEAAYRKPLEG"
     gene            complement(2336..3517)
                     /locus_tag="VFV59_09310"
     CDS             complement(2336..3517)
                     /locus_tag="VFV59_09310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5326064.1"
                     /translation="MTSLATPPVAEPAGRTRAPLRVGIVSPYGYPHPGGVNEHVRFTY
                     EAMRRLGHDVWIITSKYGRERESEGHIIRLGTGWAWPTNGSVGRVTLGLRFKKQAREI
                     LQAHRFDILHFHEPLVPFLSPTVLDASETVNIATFHAFGGFSPSYWIGKRFASRLADK
                     LDGRIAVSGAARHFISRYFPGDYRIIPNGVDLDRFAQAEPFEELRDGTLNILFVGRFE
                     ERKGLIHLLKAYHRLRKRKVDARLLVVGGGPKEREYRRFIGLRGIRDVEFLGRVSDED
                     KVRYFASADIFCAPNTGQESFGIVLLEAMAAGIPIVASDIHGFKNVVQRNVQGILVEP
                     RNPRALAAGLYALARDPDLRHEMGEAGRARAPEYTWDRVTEQIVDYYHEVRDRVLASR
                     G"
     gene            complement(3514..4125)
                     /locus_tag="VFV59_09315"
     CDS             complement(3514..4125)
                     /locus_tag="VFV59_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326065.1"
                     /translation="MAESDRRWRVAGWVVNGALRAAIVGFVVEAATASAGDPRFAGKG
                     IPVRNFVFGGGALTLAFPLLHRSVRPWDRYPVWADALFLSILALDMAGNSLDLYERGW
                     RFDLIPHTYGPAAGVATLRVVGVELLPGALLVNATHLLLEIQEALGDALFGTHNVHGW
                     WDTISDLGAGVVGSVGIPLLVQRVRRHRARGRLAPRHRSYSKA"
BASE COUNT          608 a         1452 c         1586 g          596 t
ORIGIN      
        1 ggggaccaga tggtgttcgt gatccagtcg atcatcctgc tgttcagcgg cgtctactac
       61 gagaccaccg ccctccccga ctggatgcag ttcatctcgc agttcagccc gggcacctac
      121 gtgctcgacg gcgtgcgagc cgggctgatc tacaacaagc cggtgacgga gctgtggaac
      181 gacgtgtggc cgctgctgct gatgggcctc atcctcatcc cggtcggcgt ccgggtgttc
      241 ggcatggccg agcgctacgc gaagcgcacc gggaagctca agcgcgtcgg atgaccgcgg
      301 cgcgaccgga gacgaccgac atgcagaccg atacggccgc gcacgcggcc acccttcccg
      361 gcctcgtcgt gcgccccttc gccggggagg ccgacctggc cgagatcgcg cgcatccaga
      421 acgccgagtg ggaggccgac ggcgtgcgcg gccgcaccac gcccgaggag ctcgccgcct
      481 ggctgagcca cccctcggag cagttcgacg cgcggcgcga cgtcagcctc accgaggtcg
      541 acggccgcgt cgtggccgtg acgcaggtgg actggatcga cgccaccgac ggcgtgcgcg
      601 agtaccgctc gcgctgctgg ctggaccccg tgtaccgacg ccgggggatc ggcaccatgc
      661 tcctgctgcg cagcgagcag cgacgccggc agatggccgc tgggcacgac acggatcggc
      721 cgaaggtgct cggcgtcgga accgccgaga cgaacctcgg cggcaacgcg ctgttccggc
      781 gcttcggcta cgagccagtt cgctggttct tcgacatgga gcgggcgatc gacggcgggc
      841 tgccggagat cccggccctc cctgcgggga tcgaggtgcg gccggtcggc cgggagcacg
      901 cccgacaggt gtgggaggcg gaccacgatg ccttccgcga tcactggggc ggcgccgacg
      961 cctcggacgc gagcttccag cgctggctgg actcgccgga gttcgacccg tcgctgcagg
     1021 tcgtggcgtg ggacggcgac gagatcgcgg gcgcggtgct gaacgccatc taccccgagg
     1081 agaacgaggc gctgggcgtc cgccgtggct ggctcgactc ggtcttcacc aggcgagcgt
     1141 ggcgacgccg cggcctggcg cgcgcgctga tcgtccgctc gctgcacctg cttcgcgagc
     1201 gcgggatgga gggcgccgtc ctgggcgtcg acgcggacaa cccgtccggc gcgctgggcc
     1261 tgtacgagtc ggtcggcttc gccgcaaccg agcggttcgt ggcgtaccgg cggccgctgg
     1321 aggtgccgtc cccgtgagcg tggtcctgac ggcggccccg gccatcccgg ggctgcgctt
     1381 ccgcccctac gccggacccc aggaccaccc ggagatgcac cgggtgcacc aggccgtggc
     1441 cgccgcgaac ggcatcgagg aggtcatctc gctggagcag ttcgacctgt cctacgccaa
     1501 tcacgtgaac tgcgaccacg accgcgacat cgtgctggcc gaggtcgacg gcaacctggt
     1561 ggcgtacagc cgggtcttct ggtccgagtt gctcgagggc ggccgatcct acgagaactt
     1621 cgggttcgtc caccccgagt ggcggcgacg ggggatcggc acggccatgc atcggcacaa
     1681 cgaggcgcgg ctccgggaga tcgccggcgg ccacgccggc gtgggtccga agtggctgtc
     1741 gtccgaggcc gtcgacggcg accccggcgc ggccgcgctg ctccgggccg acggctacga
     1801 gcccgtgcgc tggttcttcg agatggtggc gccctcgctg gaggacgtgc cctcggttgg
     1861 cgtgcctgac ggagtcgagg tgcgaccggt gacgcgcgag cagtaccgcc ggatctggga
     1921 cgccatggac gaggccttcc gcgaccactg gggcgcgact ccggggaccg aggaggagtg
     1981 gcgcacgttc gagggcgatc ccgacaacgc cgacccgcgc tactggcgcg tggcgtggga
     2041 tggcgacgag gtcgccggcg tggtggtcac ggtgccccag acggaggcga acgcgcggca
     2101 tcagcggcgg cgggtgtacg tcgcctcggt ctcggtgcgg cgtccctggc gacggcgcgg
     2161 gctggcgcgg gcgctgctcg ccggctcgct gatcggggcg cgcgaggacg ggttcacctc
     2221 ggccagcctg ggcgtcgacg cggagaaccc gaccggggcg ctggcgctgt acgaatcgct
     2281 cggcttcgag cggcaccgtt ccgaggcggc gtaccggaag ccactcgagg gctgatcagc
     2341 cccgactggc gaggacccgg tcgcggacct cgtggtagta gtcgacgatc tgctcggtga
     2401 cccgatccca ggtgtactcg ggtgcgcgag cgcgacccgc ctcgcccatc tcgtggcgca
     2461 ggtcggggtc acgcgccagg gcgtagaggc ccgcggccag cgcccgcggg ttgcgcggct
     2521 cgaccaggat gccctggacg ttgcgctgca ccacgttctt gaagccgtgg atgtcggagg
     2581 cgacgatcgg gatgccggcg gccatcgcct ccagcaggac gatgccgaac gactcctgtc
     2641 ccgtgttcgg cgcgcagaag atgtccgcgc tggcgaagta gcggaccttg tcctcgtcgc
     2701 tgacgcgtcc gaggaactcg acgtcgcgga tgccgcgcag cccgatgaac cgccgatact
     2761 cccgctcctt cgggccgccg cccacgacca gcagtcgcgc atcgaccttg cgcttgcgca
     2821 ggcggtggta ggccttgagc aggtggatca ggcccttgcg ctcctcgaag cggccgacga
     2881 agaggatgtt gagcgtgccg tcgcgcagct cctcgaacgg ctcggcctgc gcgaatcggt
     2941 cgaggtcgac gccgttgggg atgatccggt agtcgccggg gaagtatcgg ctgatgaagt
     3001 ggcgggcggc gccggagacg gcgatccggc cgtccagctt gtccgccagc cggctcgcga
     3061 accgcttgcc gatccagtag ctggggctga acccgccgaa ggcgtggaag gtggcgatgt
     3121 ttaccgtctc ggacgcgtcc agcacggtcg gggagaggaa gggcaccagt ggctcgtgga
     3181 agtgcaggat gtcgaaccga tgcgcctgca ggatctcgcg cgcctgcttc ttgaaccgca
     3241 gacccagggt gacgcggccg acgctgccgt tggtcggcca cgcccagccg gtgccgaggc
     3301 ggatgatgtg gccctcgctc tcgcgctccc ggccgtactt gctggtgatg atccagacgt
     3361 cgtgacccaa ccgccgcatc gcctcgtagg tgaagcgcac gtgctcgttg acgccgcccg
     3421 gatgcgggta gccgtagggg ctgacgatgc cgacgcgcag gggagcgcgc gtacgtcccg
     3481 ccggctcggc gacgggcggc gtggcgagcg aggtcacgcc ttcgagtatg accgatgccg
     3541 cggcgcgagt cgcccgcgcg cccggtggcg ccgcacccgc tgcacgagca gcgggattcc
     3601 gaccgagccc acgaccccgg cgccgaggtc cgagatcgtg tcccaccagc cgtggacgtt
     3661 gtgcgtgccg aacagcgcgt cgccgagcgc ctcctggatc tcgagcagca ggtgcgtggc
     3721 gttgaccagc agggctcccg ggagcagctc cacgcccacg acgcgcaggg tcgcgacgcc
     3781 ggcggccggc ccgtaggtgt gcgggatcag gtcgaaacgc cagccccgct cgtacaggtc
     3841 cagcgagttg ccggccatgt cgagcgcgag gatcgagagg aagagggcat cggcccagac
     3901 cggatagcga tcccagggtc gaacggaccg atgcagcagc gggaaggcga gcgtgagcgc
     3961 cccgccgccg aagacgaagt tccggaccgg aatccccttc ccggcgaagc gggggtcacc
     4021 ggccgacgcc gtcgctgcct cgacgacgaa gccgacgatg gcggcgcgga gggcgccgtt
     4081 gaccacccag ccggccacgc gccagcgacg atcggactcg gccatgcccg gatcgtcgcg
     4141 ttcgcctgcg cggccggcga gggccgaatg ggccgttcgg atcggtcctc gcggaccggc
     4201 tcagcgccag tcgtcgtggc ccggcttgac cgtgtgccag ac
//
LOCUS       DASTYP010000211         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1184830, whole genome shotgun sequence.
ACCESSION   DASTYP010000211 DASTYP010000000
VERSION     DASTYP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184830"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFV59_09320"
     CDS             complement(<1..656)
                     /locus_tag="VFV59_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5326066.1"
                     /translation="MTGVWTEVGDRVWVRRYAALDQTIGVIGGHPGLLVIDTRATHQA
                     ADELRAELRQLPGDVVGVINTHGHWDHVFGNARFLPAPIHGHVRCAEMVTLTGEEQRR
                     RVMERYPPAVAEQLAEVVLTPPTELFEETAAIALEDRDVELRYLGRGHTDNDIVVRVL
                     DASVLFTGDLLENAPAPAYGDAFPIAWAETGRRLLSLVDGAVVPGHGDPFDVHFAERQ
                     V"
     gene            complement(653..973)
                     /locus_tag="VFV59_09325"
     CDS             complement(653..973)
                     /locus_tag="VFV59_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018301401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU5326067.1"
                     /translation="MIQTTTPTVDGRTITEYLGVVTGEAILGANIVRDLFAGIRDIVG
                     GRSGAYEEELRNAREIALREMEAEAASRGADAVVGIDLDYETVGQGSMLMVTASGTAV
                     RLGA"
     gene            complement(991..1368)
                     /locus_tag="VFV59_09330"
     CDS             complement(991..1368)
                     /locus_tag="VFV59_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5326068.1"
                     /translation="MNRSDVQAWLDRYVAAWAANDPDLIGDLFTEDVVYRYRPWDSDR
                     TTVRGRDAVVASWLEEPDEPGTWNAHYEPFAVEGDRAVAVGISDYPGREGGETYHNTF
                     LMRFGPDGRCAEFTEFWILQPKS"
     gene            complement(1378..>2326)
                     /locus_tag="VFV59_09335"
     CDS             complement(1378..>2326)
                     /locus_tag="VFV59_09335"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5326069.1"
                     /translation="IGGLQTQNARSGYIGLWSRLEGLRKADYTAALNERRLIQGWLMR
                     VTIHTVTADDYWPMAIAVRQPRRAWWLRTWGGGADDAAVRRVADAVAEELADGPLRQR
                     ELQARLAERGFPKELINGAALWVDLVRVPPQGTWDRPRADNYGLAADWLAPIEMSEEA
                     AQERLVGRYLAAYGPAAMSDVAGWTGIPVKALRPILERLDLRRFRDESDHELIDVLDA
                     PLPEPETPAPPRFIGSFDPILMVHARRTGILPEEYRTIVYALNAPQSFHTFLLDGKVA
                     GTWRFEDGEIRLAPFRDLSALERTALEEEAHRLGAFHAE"
BASE COUNT          323 a          841 c          821 g          341 t
ORIGIN      
        1 acctggcgct cggcgaagtg gacgtcgaac ggatcgccgt gaccgggaac caccgccccg
       61 tcgaccaacg acagcagccg tcggccggtc tccgcccagg cgatcgggaa cgcgtcgccg
      121 taggccgggg ccggcgcatt ctcgagcagg tcgccggtga acaggaccga tgcgtccagc
      181 acccgcacca cgatgtcgtt gtcggtgtgc ccgcggccca ggtagcggag ctccacgtcg
      241 cggtcctcga gcgcgatggc ggcggtctcc tcgaacagct cggtcggggg cgtgagcacg
      301 acctcggcca gctgctcggc aaccgctggt gggtagcgct ccatgacccg ccgccgctgc
      361 tcctcgccgg tgagggtgac catctcggcg catcggacat ggccgtggat gggcgccggc
      421 aggaagcgag cgttgccgaa gacgtgatcc cagtgcccgt gggtgttgat gacgccgacc
      481 acgtcgcctg ggagctgccg cagctcggca cgcagctcat cggccgcctg gtgggtcgcc
      541 cgcgtgtcga tgaccagcag cccagggtgc ccgccgatga cgccgatggt ctggtccagc
      601 gccgcgtagc gccggaccca gacgcggtcg ccgacctccg tccagacgcc ggtcacgcgc
      661 cgagtcgcac ggccgttccc gacgcggtga ccatcagcat gctgccctgg cccaccgtct
      721 cgtaatcgag gtcgatgccg accacggcat cggcgccgcg cgatgccgcc tcggcctcca
      781 tctcgcgcag cgcgatctcc cgcgcgttgc gcagctcctc ctcgtacgcg ccggagcgcc
      841 cgccgacgat gtcgcggatg cccgcgaaca ggtcgcgcac gatgttggcg cccaggatcg
      901 cctcgccggt caccacgccg aggtactcgg tgatggtccg cccgtcgacg gtcggggtgg
      961 tggtctggat catcggtctc gctccttcgg tcaggacttc ggctggagga tccagaactc
     1021 ggtgaactcc gcgcacctgc cgtccggccc gaaccgcatc aggaaggtgt tgtggtaggt
     1081 ctcgccgccc tcccggcccg ggtagtcgct gatccccacc gccaccgcgc ggtcaccctc
     1141 gacggcaaag ggctcgtagt gggcgttcca cgtccccggt tcgtcgggct cctcgagcca
     1201 ggaggcgacc acggcatcgc ggccacggac cgtggtgcgg tcgctgtccc acggccggta
     1261 tcggtagacg acgtcctcgg tgaagaggtc gccgatcagg tcggggtcgt tcgcggccca
     1321 ggccgcgacg tatcggtcca gccaggcctg gacgtccgag cggttcatcc gctgatgcta
     1381 ctcggcgtgg aaagcgccga gccgatgcgc ctcctcctcg agcgccgttc gctcgagggc
     1441 cgacaggtcg cggaacggcg ccagccggat ctcgccgtcc tcgaagcgcc aggtcccggc
     1501 caccttccca tccaacagga aggtgtggaa cgactgcggc gcgttgaggg cgtacacgat
     1561 ggtgcggtac tcctccggca ggatcccggt acgtcgcgcg tggaccatga ggatggggtc
     1621 gaacgagccg atgaagcgag gcggcgccgg cgtctcgggc tccggaagcg gggcgtccag
     1681 gacgtcgatg agctcgtggt cgctctcatc acggaagcga cggaggtcca ggcgttccag
     1741 gatcggccga agcgctttca ccgggatgcc ggtccagccg gcgacgtcgc tcatcgcggc
     1801 cggaccgtag gccgcgaggt agcggccgac gaggcgctcc tgcgcggctt cctccgacat
     1861 ctcgatcggc gcgagccagt cggccgcgag cccgtagttg tcggcgcggg ggcgatccca
     1921 ggtgccctgg ggcggcacgc gcaccaggtc gacccacagc gcggcgccgt tgatcagctc
     1981 cttcgggaac ccgcgctccg cgaggcgcgc ctggagctcg cgctgccgga gcgggccgtc
     2041 cgcgagctcc tcggcgacgg catccgcgac ccgccgaacc gccgcgtcgt cggcgccgcc
     2101 accccaggtg cgcagccacc aggcccgccg gggctgccgg acggcgatgg ccatcggcca
     2161 gtagtcgtcg gccgtcacgg tgtggatggt gacccgcatg agccaaccct ggatcagccg
     2221 gcgctcgttc agcgccgcgg tgtagtccgc cttgcggagg ccctcgagcc gcgaccacag
     2281 cccgatgtag cccgatcgcg cgttctgcgt ctgcagcccg ccgatc
//
LOCUS       DASTYP010000212         5054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_457102, whole genome shotgun sequence.
ACCESSION   DASTYP010000212 DASTYP010000000
VERSION     DASTYP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457102"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1258
                     /gene="ispG"
                     /locus_tag="VFV59_09340"
                     /gene_synonym="gcpE"
     CDS             5..1258
                     /gene="ispG"
                     /locus_tag="VFV59_09340"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5326070.1"
                     /translation="MTLPVRRPTTTVLVGGVPVGSTHPIVVQSMTNTDTADADATAIQ
                     VAQLAHAGSEIVRITVNNDEAARAVPTIREKLDRLGVGVPLIGDFHYNGHLLLTKYPE
                     MAAALAKYRINPGNVGTKRRDEQFAQIVEVAIANGKPVRIGVNWGSLDQQLLTEMMDA
                     NAASPEPVDSREVMIEAMIESALRSARLAEETGLPHDRIILSAKVSGVQDLVEVYARL
                     ADRCDYPLHLGLTEAGMGAKGIITSTAGLAILLQRGIGDTIRVSLTPEPGGDRTQEVQ
                     VAQQVLQSMGLRSFLPQVSACPGCGRTTSTFFQEMARDIQAYLVEQMPHWRETHPGVE
                     ELRVAVMGCVVNGPGESKHADIGISLPGTFEEPVAPVFVDGEKATTLRGDDIVPRFLA
                     ILDDYVDRRYPAPGSGVPRPAEPVG"
     gene            complement(1268..1663)
                     /locus_tag="VFV59_09345"
     CDS             complement(1268..1663)
                     /locus_tag="VFV59_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326071.1"
                     /translation="MPVAVGPGGPDPAGPPPPQIGPTLVLQLTNRSENALAVGYAFEG
                     NNASGGGEGSIEPCQAVAMGFGEVAGHYRVLVGGEAVGEADVPPGFGRFVFLIVDVSI
                     DADGTVETRPHRISRVQPPMMPRAVARCG"
     gene            complement(1741..2481)
                     /locus_tag="VFV59_09350"
     CDS             complement(1741..2481)
                     /locus_tag="VFV59_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5326072.1"
                     /translation="MNGVALALRQVAYENRAFWRNPAAAFFTFAFPLLFMFIFNVLFG
                     EGAGRFFTPAIIVFAVVTATFTNLAMTITIARDEGVLKRIRGTPLPTWAYLAGRIGHA
                     VAIAFLLVVIVAGFGALLYDVPVPWDALPALLLVLVVAAVTFSALGLALSGVIPNADA
                     GPAIVNAIVLPALFISNVFIQMSAAPDWLDAISHVLPTRHFADAMISLYGVGGSVGVP
                     WTEIGVIAAWGVAGVLLALRFFSWEPRR"
     gene            complement(2478..3431)
                     /locus_tag="VFV59_09355"
     CDS             complement(2478..3431)
                     /locus_tag="VFV59_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5326073.1"
                     /translation="MTEPAIEVRGLRKSYGALEAVRGIDLRVERGEVFALLGPNGAGK
                     TTTVEILEGHRTRSAGDVSVLGHDPGQHDRAYRERIGIVLQQTGVDEYLTVREVVSMV
                     AGYYPKPRDVDEAIELAGLTEQANRRVRKLSGGQKRRVDLAVALAGDPELLFLDEPTT
                     GFDPSARRQAWETVRGLTELGKTIFLTTHFMDEAQALAHRLAIIADGRIVEVGSADEL
                     IGARSHASHVRFRLPDGVSPPERFGARPQASASAWELETEEPTRTLNDLTAWALAAGT
                     ELADLEVRRRSLEDVYLQLTSEAAEGAADAPAPAAPAEAGA"
     gene            complement(3452..3643)
                     /locus_tag="VFV59_09360"
     CDS             complement(3452..3643)
                     /locus_tag="VFV59_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326074.1"
                     /translation="MDSNLPATRAEAPVEVAPGVASEPVPVVEHRRPVSVAFLPFALI
                     VFVVLAFVVAGWTFFTAAY"
     gene            complement(3646..4215)
                     /locus_tag="VFV59_09365"
     CDS             complement(3646..4215)
                     /locus_tag="VFV59_09365"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5326075.1"
                     /translation="MTAGVDTGRRPRAWRLPLPYLVVAAVLPIVLLAVLGAVLLTRLP
                     TLTPAAEGQPAPDFALADLDGNPVRLEDLRGRPVVLNFWASWCGPCVEEFPLLRAAAD
                     QHASDGLAIVGIVYDDRSEAARAFMKHNGATWPAAMDPGGRVAAAYGVYAPPQTYFIG
                     RDGTVVARQIGQFSQASLNEKLAAIIDEE"
     gene            complement(4223..4570)
                     /locus_tag="VFV59_09370"
     CDS             complement(4223..4570)
                     /locus_tag="VFV59_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326076.1"
                     /translation="MSDSAIGLIILGTAMAVVLVAVFVIASRTARPVPESAPPRGVHM
                     PSPSLLPVIMSLGAILLGAGLAFRGDAEIANPWVAIPGLVILISGIVGWVRAANREWT
                     GVEHGSHDDGAAH"
     gene            complement(4584..>5054)
                     /locus_tag="VFV59_09375"
     CDS             complement(4584..>5054)
                     /locus_tag="VFV59_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU5326077.1"
                     /translation="YFNARAVTGVENWHAPEGAHLDIPLAAALTAVLVASSFTMQFGV
                     WAIRRGDTGGLRMWTAITLVLGILFLAGQLYDYSQLGFGISDGVFGTTFYTLTGFHGA
                     HVFGGVVGLTIVLARTLSGQFSARNHVAVEAVSMYWHFVDVVWIAVFTTIYILG"
BASE COUNT          809 a         1836 c         1751 g          658 t
ORIGIN      
        1 caccatgacg ctccccgtgc gacggccgac cacgaccgtc ctcgtcggcg gcgtgccggt
       61 cggctcgacc cacccgatcg tcgtgcagtc gatgaccaac accgatacgg ccgatgccga
      121 cgccaccgcc atccaggtcg cgcagctcgc ccacgccggc tccgagatcg tgcgcatcac
      181 ggtcaacaac gacgaggccg cgcgcgccgt cccgaccatt cgcgagaagc tggaccgcct
      241 gggcgtcggc gtgcccctca tcggtgactt tcactacaac ggccacctgc tgctgacgaa
      301 gtaccccgag atggcggcgg ccctcgccaa gtaccgcatc aacccgggca acgtcggcac
      361 gaagcgtcgc gacgagcagt tcgcgcagat cgtcgaggtc gcgatcgcga acgggaagcc
      421 ggtgcgcatc ggcgtcaact ggggatcgct ggaccagcag ctgctgaccg agatgatgga
      481 cgccaacgcg gcgtcgccgg agccggtgga ctcgcgcgaa gtgatgatcg aggcgatgat
      541 cgaatcggcc ctgcgctccg cgcggctggc cgaggagacc ggcctgccac acgaccggat
      601 catcctctcg gccaaggtgt cgggggtgca ggacctggtc gaggtctacg cgcgcctggc
      661 tgaccgatgc gactatccgc tgcatctcgg cctcaccgag gccggcatgg gcgccaaggg
      721 catcatcacg tcgacggccg gcctggcgat cctgctgcag cgcggcatcg gcgacaccat
      781 tcgcgtctcg ctgacgccgg agccgggcgg cgaccgcacc caggaggtgc aggtcgcgca
      841 gcaggtcctc cagtcgatgg gcctgcgctc gttcctgccc caggtctcgg cctgtccggg
      901 ctgcggccgc acgacctcga ccttcttcca ggagatggcg cgcgacatcc aggcctatct
      961 cgtcgagcag atgccccact ggcgtgagac ccatccgggc gtcgaggagc tgcgcgtcgc
     1021 cgtgatgggc tgcgtggtca acggtccggg cgagtcgaag cacgccgaca tcggcatcag
     1081 cctgcccggc acgttcgagg agcctgtggc gccggtcttc gtcgacggcg agaaggccac
     1141 cacgttgcgt ggcgacgaca tcgtcccgcg cttcctggcc atcctcgacg actacgtcga
     1201 tcggcggtac cccgcccccg gctctggcgt gccacgtccc gccgagccgg tcggctgacg
     1261 gccgaaatca gccgcagcgc gcgacagctc tgggcatcat cggcggttgg accctcgaga
     1321 tccggtgcgg gcgtgtctcg accgttccgt cggcatcgat cgagacgtcg acgatgagga
     1381 acacgaaccg tccaaagccg ggtggaacgt ccgcttcccc caccgcctcg ccgccgacca
     1441 gcacgcggta atgtcccgca acctcgccaa accccatagc cacggcctgg cagggctcga
     1501 tggagccctc gccaccgccg gaggcgttat tgccctcgaa cgcatagccg actgcgaggg
     1561 cgttctccga tcggttcgtg agctggagca ccagcgtcgg accgatctgc ggcggcggcg
     1621 ggcctgcggg gtctggccct cccgggccga cggcgacggg catgcagccg gcggcgaggc
     1681 cgaggaccgc cgcgagcgac gccatcgggc cgacccaccc gcctcgcccc ccgaacacgg
     1741 ttagcgcctg ggctcccagc tgaagaagcg aagcgccagc aggaccccgg ctacgcccca
     1801 cgccgcgatg acgccgatct cggtccacgg cacgccgacg gagccgccga cgccgtagag
     1861 cgagatcatc gcgtcggcga agtgccgcgt cgggaggacg tgcgagatgg catccagcca
     1921 gtcgggcgcc gcagacatct ggatgaagac gttgctgatg aagagcgccg gcagcacgat
     1981 cgcgttcacg atggcgggac cggcatcggc gttggggatg acgcccgaga gcgcgagtcc
     2041 cagcgcgctg aaggtgaccg cggccaccac cagcaccagg agcagcgcgg ggagcgcgtc
     2101 ccagggcacc ggcacgtcgt agaggagcgc cccgaacccg gcaacgatga ccaccagcag
     2161 gaacgcaatc gccacggcat gaccgatgcg ccccgccagg taggcccagg tcggcagtgg
     2221 cgtgccgcgg atccgcttca gcaccccctc gtcgcgcgcg atggtgatcg tcatggcgag
     2281 gttggtgaac gtcgccgtca cgacggcgaa gacgatgatg gccggcgtga agaaccggcc
     2341 ggcgccctcg ccgaagagga cgttgaagat gaacatgaac agcagcggga acgcgaaggt
     2401 gaagaacgcc gccgcggggt tgcgccagaa ggcacggttc tcgtaggcga cctggcgcag
     2461 ggccagcgcg acgccgttca tgcgcccgcc tctgccggcg ccgcgggcgc cggcgcatcg
     2521 gctgcgcctt cggcggcctc actggtgagc tgcaggtaca cgtcctcgag cgaccgacgc
     2581 cgcacctcca ggtccgccag ctccgtcccg gccgccagcg cccatgccgt caggtcgttg
     2641 agcgtccgtg tcggctcctc ggtctccagc tcccacgccg aagccgatgc ctgcggtcgc
     2701 gcgccgaagc gctcgggcgg cgacacgccg tcgggcaggc ggaaccgcac gtgggaggcg
     2761 tgcgatcgcg ccccgatcag ctcgtcggcc gagccgacct ccacgatccg gccgtcggcg
     2821 atgatcgcca gccgatgggc cagcgcctgc gcctcgtcca tgaagtgcgt ggtcaggaag
     2881 atcgtcttgc ccagctcggt gagcccgcgc accgtctccc acgcctgcct gcgggcggac
     2941 gggtcgaagc cagtcgtcgg ctcgtccagg aagaggagct ccgggtcgcc ggccagcgcc
     3001 acggccaggt ccacgcgccg cttctgcccg ccggacagct tccgcacgcg acggttcgcc
     3061 tgctcggtga ggccggccag ctcgatcgcc tcgtcgacgt cgcgcggctt cgggtagtag
     3121 cccgcgacca tcgagacgac ctcccggacg gtgaggtact cgtccacgcc ggtctgctgc
     3181 agcacgatcc cgatgcgctc gcggtacgca cggtcgtgct gaccggggtc gtgccccagc
     3241 acgctcacgt cgccggccga gcgcgtgcgg tgaccctcca ggatctcgac ggtggtcgtc
     3301 ttgccggcgc cgttcgggcc cagcagcgcg aacacctcgc cgcgctccac gcgcaggtcg
     3361 atcccgcgca cggcctccag cgcgccgtac gacttgcgca gcccccgcac ctcgatcgcc
     3421 ggctcggtca cggcggctgc cctcctcggc gtcagtaggc ggcggtgaag aaggtccagc
     3481 ccgcgaccac gaaggccagc accacgaaga cgatcagcgc gaagggcagg aaggccacgc
     3541 tgaccgggcg gcggtgctcc accacgggca ccggctcgct tgcgacgccc ggcgcgacct
     3601 cgaccggcgc ctcggcccgg gtggcgggaa ggttcgagtc catggtcact cctcgtcgat
     3661 gatggcggcc agcttctcgt tgagcgacgc ctgcgagaac tggccgatct ggcgagccac
     3721 gacggtcccg tcgcgaccga tgaagtaggt ctggggtgga gcgtacacgc cgtacgccgc
     3781 cgcgacacgc cccccgggat ccatggccgc cggccacgtc gcgccgttgt gcttcatgaa
     3841 ggcgcgggcg gcctcggatc gatcgtcgta cacgatcccc acgatcgcca gcccgtcgct
     3901 cgcgtgctgg tccgccgcgg cgcgaagcag ggggaactcc tcgacgcacg gcccgcacca
     3961 gctggcccag aagttcagca cgaccggacg gccacgcagg tcctcgagcc gaacggggtt
     4021 gccgtccagg tccgccagcg cgaagtccgg cgcgggctgg ccctccgccg ccggcgtcag
     4081 ggtcggcagg cgcgttagca gcaccgcccc gagcaccgcc agcaggacga tggggaggac
     4141 cgccgccacg accaggtatg gcagtggcag gcgccacgcg cgcggccggc gtccggtgtc
     4201 gacgccggcc gtcatcggtc cctcagtgcg cagcgccgtc gtcgtgggat ccgtgctcca
     4261 cgccggtcca ctcgcggttc gcggcgcgaa cccagccgac gatgcccgag atcaggatca
     4321 ccagcccggg gatggcgacc cacgggttgg cgatctcggc atccccgcgg aacgccagcc
     4381 cggcgccgag gaggatcgcg cccagggaca tgatcaccgg gagcaggctg ggcgacggca
     4441 tgtgcacgcc gcgcggcggg gcgctctccg gcacgggccg cgcggtgcgc gacgcgatga
     4501 cgaacaccgc caccagcacc accgccatgg cggtgcccag gatgatcagg ccgatcgcgg
     4561 agtcggacat gcggatcgct cccctagccc aggatgtaga tcgtcgtgaa gacggcgatc
     4621 cacaccacgt cgacgaagtg ccagtacatg ctgaccgcct cgaccgccac gtggttgcga
     4681 gcgctgaact ggccgctcag cgtcctggcc aggacgatcg tcagacccac aacgccgccg
     4741 aacacgtggg cgccgtggaa gccggtcagc gtgtagaagg tcgtcccgaa gacgccgtcc
     4801 gagatgccga agcccagctg gctgtagtcg tacagctggc cggccaggaa caggatgccc
     4861 aggacgagcg tgatcgccgt ccacatccgc agcccgccgg tgtcgccgcg gcggatggcc
     4921 cacacgccga actgcatcgt gaagctggag gcaacgagga ccgcggtcag cgcggcggcg
     4981 agggggatgt ccaggtgcgc gccctcaggc gcgtgccagt tctcgacgcc ggtgacggcc
     5041 cgcgcgttga agta
//
LOCUS       DASTYP010000213         7030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1294639, whole genome shotgun sequence.
ACCESSION   DASTYP010000213 DASTYP010000000
VERSION     DASTYP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294639"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VFV59_09380"
     CDS             <1..1206
                     /locus_tag="VFV59_09380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5326078.1"
                     /translation="FAERWNRRKAREAEQRAAEAETEEAAVETETDDETLPERIRKAP
                     PEGRTETREPLYDEGDVDTLMGQMRGVRYDEEIEVADGIRAIFHDAGHILGSAIIELR
                     LTDGDASRTIVFSGDLGRDGSPILRDPAPLTHADHVVVESTYGNREHAPHDEAIDELV
                     AAVGEVANDEGVLLVPAFAIGRTQELIWVLDELVRQDRIPRVPLYLDSPMASKATTVY
                     TDHPEVYDDETRDLLTSGDSPLNYPGQEFTDSVEQSKAIRHARRPIMVVAASGMLTGG
                     RIMHHLKDFLPDPTCTLLFIGYQGEGTLGRHLQNGGKTARIDGQEIQVRCRVRSISGF
                     SAHADQHELEAWLGHFGRAGNASDGRPKRVFVVHGDPDAAEAFATRIRDELGMDPHIA
                     RHRERVSLT"
     gene            1275..2114
                     /locus_tag="VFV59_09385"
     CDS             1275..2114
                     /locus_tag="VFV59_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU5326079.1"
                     /translation="MTDARPVIGFLTDFGLDGAAATCRAVMLSICRDAQIIDIAHTIR
                     KYAIRDGAFLMRFALPYAPVGVHCGVVDPGVGTQRRPIGIRTGRGDVLIGPDNGLLPP
                     VADALGGAVEARELTNRSLWLPTTTTTFHGRDLFAPVTAHLANGDASFEEVGPTLPLE
                     ELVRLPEPAAQAARGRIDTVVTYVDSFGNVRLAGGRAELEAALGSPSDGRPLVVEFEA
                     APGAPAVREETRFGSTFGAVEQGASVVYLDSLGNLALADNQGNLAARLGIGPDRRVRV
                     TVP"
     gene            2111..2923
                     /locus_tag="VFV59_09390"
     CDS             2111..2923
                     /locus_tag="VFV59_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU5326080.1"
                     /translation="MSGPFVSFLTDFGDTAPATCRGVIWTISPDARINDLSHGGRQFG
                     IRDGAFLLWSAVPYQPVGVHLAVVDPGVGTHRRAIALQSARGDRFVGPDNGLLLPAAE
                     RLGGIVAAHVLENRALWRAEVSNTFHGRDIFSPVAAHLANGTPIHEVGPPIAVEDLVP
                     LSFPEPHHSDGALKTSVLYIDAFGNCRLAGVASDLERLTGRLEPGDRFLVSMGRKRVD
                     LPWATTFGAVNLGEAILYDDADYAGLAVGVNQGSAAERFGLAHDHPVRIEPA"
     gene            2926..4617
                     /locus_tag="VFV59_09395"
     CDS             2926..4617
                     /locus_tag="VFV59_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5326081.1"
                     /translation="MPAGPLVEVDGLTFRYRRGPAPVVRDISMAVHPGEVILVAGPSG
                     CGKSTLLRAINGLVPHAYSGELEGAVRIAGRLTTEMRLREISEVVGTLLQDPAKQIVG
                     ATVESELAFGPENMGIAPADIRRRMAEVMAETEIGHLGGRDTAHLSGGERQLLAMAGA
                     LMLRPRCYVVDEPLANLDPASATRLLDLLRRLADAGHAVVIVEHRVEEALQLAPDRVV
                     AMAEGRVTYDGPVDGFFRVADPDAVKLPFPVVLDRLRGGGVASRAVEPTRVPQQRAAD
                     EARVTFDDVHVTLGERQVLRGVDSTLGRRETVAVLGPNGSGKTTLFRTAMGLVQPEAG
                     SVTVDGRDVASTPVAELARSIGYVFQSPSQMLFARTVREELLFGPRNLGLEIGDGDAL
                     AIEALTRVGLADEPDILDRPPMTLSFGQQKRLALAIGLALRPRTLILDEPSAGQDYRT
                     AEAFMREVARIPGLESVYFITHDVDLALTHADRILVLRDGRIVADGPPATVVEDAENW
                     HASNLQRTSLMDANLAWDGARGARFLDAHALAAALIARERPTSEGEEEGRRSIDS"
     gene            4653..5222
                     /locus_tag="VFV59_09400"
     CDS             4653..5222
                     /locus_tag="VFV59_09400"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5326082.1"
                     /translation="MTATTMREEAGAGMWEVTTRVIVYAAIGAALYGVLAFFSILIPG
                     TANVAVRPAFALVTFFGFAFGPIVGFFTGLVGNMIADQISGWGLLTSWNWSVANGLVG
                     LLTGVFGVYLARTIPNRLLLAAVASALAIVIGFLFVITDIWLGTADDLGVALSANYFP
                     VIIANLIASVILTPLLVAAWDPVRESMGR"
     gene            5251..6102
                     /locus_tag="VFV59_09405"
     CDS             5251..6102
                     /locus_tag="VFV59_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU5326083.1"
                     /translation="MNVAPRYLGRGSWLSRRDPRLLLVALALFVFTVIQVWDLRILAV
                     LAAIAAIYYRSARIPFRAVRIQWFYVLFFITFVVVVNTLLTGGELRGYTEDELHVFFR
                     LPLLGTPISAESVTYAIAQYARFLSMAAFGLPIAYAIAPGDIGPAFARLGVPYKFAYG
                     MELTFRFIPSLGADMQTTIDAQRLRGYEWEKRGRTPIGKLTRTVPLITPVTINAIVGA
                     EDTIDAMDLRGFGTQRRTWLRELAFDRTDRLVLLAFVALLGVVTVLSFSGQTQLWVPP
                     VLVDLAG"
     gene            6348..>7030
                     /locus_tag="VFV59_09410"
     CDS             6348..>7030
                     /locus_tag="VFV59_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326084.1"
                     /translation="MTRDAYPPLRPQPPVRPARRRRRSTPPPSTLAGAVVLLVFIVVV
                     AALLWSNRGDGGTAVDPSASVPVPSGGAVPTGGASIPATPLPSSSAVASGTPAPGFAA
                     DSLVTLTADALTLRAEPGIGAAVLARLPAGQTAFVLAGPQITDGLPWYHLSGLGLPYG
                     SGCTEPAPGETLTCPAWIGWVAAADEAGAAWMEPAPAPACPEGPPTIVSVTEMAYTMR
                     LICYDAQDMT"
BASE COUNT          955 a         2561 c         2446 g         1068 t
ORIGIN      
        1 ttcgccgagc gctggaaccg tcgcaaggcc cgcgaggcgg agcagcgagc cgcggaggcc
       61 gagacggagg aggccgcggt cgagaccgag accgatgacg agacgcttcc cgagcgcatc
      121 cgcaaagcgc cgcccgaggg ccgtaccgag acgcgcgagc cgctgtacga cgagggcgac
      181 gtcgacacgc tgatggggca gatgcgcggc gtccgctacg acgaggagat cgaggtcgcc
      241 gacggcattc gcgccatctt ccacgacgcc ggccacatcc tgggctccgc catcatcgag
      301 ctgcgcctga ccgatggcga cgcgtcgcgc accatcgtct tctccggcga tctcggacgc
      361 gacggatcgc ccatcctgcg cgacccggcg ccgctgaccc acgcggacca cgtcgtggtc
      421 gagtccacgt acggcaaccg cgagcacgcg ccgcacgacg aggcgatcga cgagctggtg
      481 gccgcggtcg gcgaggtcgc caacgacgag ggcgtgctgc tggtccccgc cttcgccatc
      541 ggtcgcacgc aggagctcat ctgggtgctg gatgagctgg tgcggcagga ccgcatcccg
      601 cgcgtgccgc tctacctcga ctcaccgatg gcctcgaagg cgacgaccgt ctacaccgat
      661 caccccgagg tctacgacga cgagacccgc gacctgctca cctccggcga ctcgccgctg
      721 aactaccccg ggcaggagtt caccgattcg gtcgagcagt cgaaggccat ccgccatgcg
      781 aggcggccga tcatggtcgt cgccgcgtcg gggatgctca ccggcggccg gatcatgcac
      841 cacctgaagg acttcctccc cgaccccacc tgcacgctgc tgttcatcgg ttaccagggc
      901 gagggcacgc tggggcgtca cctccagaac ggcgggaaga cggcgcgcat cgacggccag
      961 gagatccagg tgcgctgccg ggtccgatcc atcagcggct tctcggccca cgccgaccag
     1021 cacgagctgg aggcctggct cggccacttc gggcgggccg ggaacgcctc ggacggacgg
     1081 ccgaagcggg tcttcgtcgt ccacggcgac ccggacgccg cggaggcgtt cgccacccgc
     1141 atccgcgacg agctcgggat ggacccgcac atcgcccgcc accgggagcg cgtgtcgctc
     1201 acctgagact ccgttcgacg cctcgcggcg gggcggctgg ttcgcggccc tcgccgcacc
     1261 ggtaggatgc gccgatgacc gacgctcgcc ctgtcatcgg cttcctcacc gacttcggcc
     1321 tcgacggcgc ggccgcaacc tgtcgggccg tgatgctgtc catctgtcgc gacgcgcaga
     1381 tcatcgatat cgcgcacacg atccggaagt acgcgatccg cgacggcgcc ttcctgatgc
     1441 gcttcgccct gccgtacgcc ccggtcgggg tgcactgtgg cgtggtggac cccggcgtcg
     1501 ggacccagcg tcggccgatc ggcattcgga ccggccgcgg agacgtgctc atcgggccgg
     1561 acaacggcct gcttccgccg gtcgccgacg cgctcggcgg tgccgtcgag gcccgcgagc
     1621 tgacgaaccg ctcgctgtgg ctgcccacga ccacgaccac gttccacggt cgcgacctgt
     1681 tcgcgccggt cacggcgcat ctcgcgaacg gcgacgcgtc gttcgaggag gtgggcccca
     1741 cgctgccgct ggaggagctc gttcgcctgc ccgaaccggc ggcacaggcg gcgcggggcc
     1801 ggatcgacac ggtggtcacc tacgtggaca gcttcggaaa cgtgcggctg gccggcggtc
     1861 gcgccgagct cgaggcggcg ctcggctcgc ccagcgacgg ccggccgctc gtggtcgagt
     1921 tcgaggcggc gcccggcgcg ccggcggttc gcgaggagac gcggttcggg tccaccttcg
     1981 gcgccgtgga gcagggcgca tcggtcgtgt acctggactc gctcgggaac ctggccttgg
     2041 ccgacaacca ggggaacctg gccgcgcgcc tcgggatcgg tccggaccgc cgcgtgcggg
     2101 tgaccgtgcc gtgagcggcc cgttcgtcag cttcctgacc gacttcggcg acacggcgcc
     2161 ggcgacctgt cgcggcgtca tctggacgat cagcccggac gcgcgcatca acgacttgag
     2221 ccacggcggt cgccagttcg ggattcggga cggcgcgttc ctgctgtggt cggccgtgcc
     2281 gtaccagccg gtgggcgtgc acctggccgt cgtcgatccg ggggtcggca cgcatcgccg
     2341 cgccatcgcc ctccagtcgg cgcggggcga ccggttcgtc gggccggaca acggactgct
     2401 cctgccggcc gccgagcggc tgggcgggat cgtcgcggcg cacgtcctgg agaaccgggc
     2461 gctgtggcgt gcggaggtgt ccaatacgtt ccacggccgt gacatcttct cgcccgtcgc
     2521 cgcgcacctg gcgaacggca cgcccatcca cgaagtcggg cctcccatcg cggtcgagga
     2581 cctcgtgccc ctctcgttcc ccgagcccca ccacagcgac ggggcgctca agacgagcgt
     2641 gctgtacatc gacgccttcg gaaactgccg cctggccggg gtggccagcg acctggagcg
     2701 gctgaccggc cggctggagc ccggcgatcg gttcctcgtc agcatgggac ggaaacgcgt
     2761 cgatctgccc tgggcgacga ccttcggcgc cgtcaacctg ggtgaggcca tcctctacga
     2821 cgacgcggac tacgccgggc tcgccgtggg cgtcaaccag ggatcggcgg ccgagcggtt
     2881 cgggctcgcc cacgaccacc ccgttcggat cgagcccgcc tgacgatgcc cgccggcccc
     2941 ctcgtcgagg tcgacggact gacgttccgc taccggcgcg gaccggcgcc ggtggtccgc
     3001 gacatctcga tggcggtcca ccccggcgag gtcatcctgg tcgccggccc ctcgggctgc
     3061 ggcaagagca cgctgctgcg cgccatcaac ggcctggtgc cgcatgccta ctcgggtgag
     3121 ctcgagggcg cagttcggat cgcggggcgg ctgacgaccg agatgcggct gcgcgagatc
     3181 agcgaggtgg tcggcacgct gctgcaggac ccggcgaagc agatcgtcgg agccacggtc
     3241 gagtccgagc tggcgttcgg cccggagaac atggggatcg cgccggcgga cattcgacga
     3301 cggatggccg aggtgatggc cgagaccgag atcggccacc tcggcggccg cgacaccgca
     3361 cacctgtccg gcggcgagcg tcagctgctc gcgatggcgg gggcactgat gctccggccg
     3421 cgctgctacg tcgtcgacga accgctcgcc aacctcgatc cggccagcgc gacccgcctg
     3481 ctcgacctgc tccgccgcct ggccgatgcg ggtcatgccg tggtcatcgt ggagcaccgc
     3541 gtcgaggagg ccctccagtt ggccccggac cgcgtggtgg ccatggccga gggccgcgtg
     3601 acgtacgacg gccccgtcga cggcttcttc cgcgtcgcgg acccggacgc cgtcaagctg
     3661 ccgttccccg tcgtcctgga tcggctgcgc ggcggcggcg tggccagccg ggccgtcgag
     3721 ccgacgcgcg tgccgcagca gcgggccgcg gacgaggcgc gcgtgacgtt cgacgacgtg
     3781 cacgtgacgc tcggcgagcg gcaggtcctt cgcggcgttg acagcacgct cggacgccgc
     3841 gagacggtcg cggtgctcgg cccgaacggg tccggcaaga ccacgctctt tcgcacggcg
     3901 atggggctcg tccagcccga ggccggctcg gtgacggtcg acgggcggga cgtcgcgtcc
     3961 acgccggtcg cggagctcgc ccgcagcatc ggctacgtct tccagagccc gagccagatg
     4021 ctgttcgcgc gcaccgtccg ggaggagctg ttgttcggac cccgcaacct gggcctggag
     4081 atcggcgacg gcgacgcgct cgccatcgag gcgctgaccc gcgtcgggct cgccgatgaa
     4141 cccgacatcc tcgatcgccc gccgatgacc ctcagcttcg ggcagcagaa gcgcctggcc
     4201 ctggcgatcg gactcgccct cagaccgcgc acgctgatcc tggacgagcc gtcggcaggg
     4261 caggactacc gcaccgctga ggccttcatg cgcgaggttg ctcgcatccc cgggctcgag
     4321 agcgtctact tcatcacgca cgacgtggac cttgcgctga cgcacgccga tcgtatcctt
     4381 gtcctccgtg acggccggat cgtcgccgat ggaccgcccg ccaccgtggt cgaggatgcc
     4441 gagaactggc acgcgagcaa cctccagcgc acgtcgctga tggacgcgaa cctggcgtgg
     4501 gacggcgctc gtggcgcacg cttcctcgac gcgcatgccc tggccgccgc gctcatcgcg
     4561 cgcgagcggc cgacatcgga gggggaagag gagggtcgtc ggtcgatcga tagctgacgc
     4621 agccaacgga ccgacacaca cggagggttt tcatgacagc caccaccatg cgagaggaag
     4681 caggcgccgg catgtgggag gtcaccaccc gggtgatcgt ctacgccgcg atcggtgcgg
     4741 cgctctacgg cgtgctcgcc ttcttcagca tcctgattcc cgggacggcg aacgtggccg
     4801 tccgcccggc gttcgcgctg gtcaccttct tcggcttcgc cttcgggccc atcgtcggct
     4861 tcttcaccgg actggtgggc aacatgattg ccgaccagat cagcggctgg ggcctcctga
     4921 cgagctggaa ctggagtgtc gccaacggcc tggtcggtct cctgacgggc gtgttcggcg
     4981 tgtacctggc gcgcacgatt ccgaaccgcc tgctgctggc ggccgtggcg tcggcgctgg
     5041 cgatcgtcat cggcttcctg ttcgtcatca ccgacatctg gctcggcacg gccgatgacc
     5101 tgggcgtggc cctctcggcc aactacttcc cggtcatcat cgccaacctc atcgcctcgg
     5161 tgatcctcac cccgctcctc gttgcggcgt gggatccggt tcgggagtcg atgggccgct
     5221 gaagagcctg ctgaacggga cggcgtcgcg gtgaacgtcg cccctcgcta cctcggacga
     5281 gggtcgtggc tgtcacgccg cgaccctcgt ctgctgctgg tcgcgctggc cctcttcgtc
     5341 ttcaccgtga tccaggtgtg ggacctgcgc atcctcgccg tgctcgccgc gatcgcggcg
     5401 atctactacc gatccgcgcg gatccccttc cgtgcggtgc gcatccagtg gttctacgtc
     5461 ctcttcttca tcaccttcgt cgtggtggtg aacacgctcc tcacgggggg cgagctgcgt
     5521 ggctacaccg aggacgagct gcacgtcttc ttccgtctcc cgctgctcgg aacgccgatc
     5581 agcgccgagt cggtgaccta cgccattgcc cagtacgcgc gattcctgtc gatggccgcc
     5641 ttcggcctgc cgatcgccta cgccatcgcc ccgggcgaca tcggcccggc cttcgcgcgg
     5701 ctcggcgtgc cgtacaagtt tgcgtacggc atggagctca ccttccgctt catcccgtcc
     5761 ctcggcgccg acatgcagac gaccatcgac gcgcagcggc tgcgcgggta cgagtgggag
     5821 aagcgcggcc gcacgcccat cggcaagctg acgcgcacgg tgccgctcat caccccggtg
     5881 acgatcaatg ccatcgtcgg cgccgaggac acgatcgacg cgatggacct ccgcggtttc
     5941 ggcacccagc ggcgcacgtg gctgcgtgag ctcgccttcg accggaccga tcgcctggtg
     6001 ctgctggcgt tcgtggcgct gctcggcgtc gtgacggtgc tgagcttctc gggccagacg
     6061 cagctctggg ttccgcccgt cctggtcgac ctcgccggct gaacgaggtt cgcgcagcaa
     6121 tcggctgcgc ggaggcgccg catcggttca cactgtggat ccatggtgga taaccgccgg
     6181 atggatcacg aatctcgtgg acaaccgggc ttggcaccgc gggccggcgc gccgtatcgt
     6241 catcgacgtc ccggaggagt ccgtggactc ggagattgca tcaacgggtc ccgtggttcc
     6301 ttcgggacgt tcttcgtgtc cggcgaatgc gccatcggag ggggcacgtg accagggacg
     6361 cctacccgcc gctgcggccg cagccgcccg tccgtcccgc gcgccggcgc cgccgctcca
     6421 ccccgccgcc cagcaccctc gccggcgccg tggtgctgct cgtcttcatc gtcgtggtcg
     6481 cggcgctgct gtggtccaac cgtggtgacg ggggcaccgc cgtcgaccca tccgcctcgg
     6541 tcccggttcc gtcgggtggt gccgtgccga ccggtggcgc cagcatcccg gccacgccgc
     6601 tccccagctc ctccgcggtg gcctcgggaa cccccgcccc cggcttcgcc gcggacagcc
     6661 tggtcacgct gaccgccgat gcgctgaccc tgcgcgccga gcccggcatc ggggcggccg
     6721 tcctcgcccg gctgcccgcc ggccagacgg cgttcgtcct cgccggaccc cagatcaccg
     6781 acgggctgcc gtggtaccac ctgagcgggc tcggactgcc ctacgggtcg ggttgcacgg
     6841 aaccggcgcc gggcgagacg ctcacctgtc cggcctggat cggctgggtc gccgccgcgg
     6901 acgaggccgg cgcggcgtgg atggagccgg cgccggcacc ggcgtgcccg gaagggccgc
     6961 cgacgatcgt ctccgtcacg gagatggcgt acacgatgcg cctcatctgc tacgacgcgc
     7021 aggacatgac
//
LOCUS       DASTYP010000214         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1767896, whole genome shotgun sequence.
ACCESSION   DASTYP010000214 DASTYP010000000
VERSION     DASTYP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767896"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..916
                     /locus_tag="VFV59_09415"
     CDS             113..916
                     /locus_tag="VFV59_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326085.1"
                     /translation="MRTDAAAVSPSLASALETLRARGLDLRRGTQPMQQAQPRPSIPS
                     GHGALDVALATGGWPRGALALLDAPAGGGATTLALGSLAAAQAGGGLAAWLDVDGTFD
                     PAVAARLGVQLDWLLIAHPTDAGEAVELAAWLGRSGLLDAVVLDLGERSGSVDRRALD
                     RLGALLARAGATSVLTVAPTARATVGAVAGVRVALRRTAWLALGRDLVGQRVHATVER
                     HRWALAGGSAELDLWFAEGRRIDALAPRLAQPQNPATTEERPALRVVGA"
     gene            952..1815
                     /locus_tag="VFV59_09420"
     CDS             952..1815
                     /locus_tag="VFV59_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5326086.1"
                     /translation="MRYAISIPNFGEWADPRTFIGLAQDAEAAGWDGFFAWDHIRFSA
                     TPLPVHDPWALLAGIATVTERLVIGPMVTPLPRRRPWVVARQAVTIDHLSNGRMMLGV
                     GIGEPADIELAAFGEATDLKVRAEMLDEGLAILDGLWSGEEFSFDGKHYHLAPMTFLP
                     RPIQRPRIPIIVAGYWPHRGPIRRAARWDGMNALFPYPLEGWEEKARALVAELRSLRA
                     DPTGPFEYFDGGHTPDDGPDADDPVLSADIGATWWIESLDPWRFTWDGSAEGWPSVEL
                     ARDRIRLGPPR"
     gene            1820..3022
                     /locus_tag="VFV59_09425"
     CDS             1820..3022
                     /locus_tag="VFV59_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326087.1"
                     /translation="MPRLLALVWPELRDPDAEVDARPWDRMLDALDDLSPRVEAVDLG
                     VALVDITGLEPMFGPERRVAARAVSLARTVAPLRVRCGIGENRWLASLAARLARFERP
                     EAPAALYILDRAELPDLPLSLLTADPKVRQRFALFGLTTMGQLANLPRSAVGAQLGPT
                     GERLQALARGHDPRPLVPRRRPERLEARDTFEPPIEGVGGVALTLRRLAADLCDRLRT
                     RHLAPGRATLTLVLEDAPALRVAQPFPQPALEPDWIARLLLSRVEAAARLRSCRGAEL
                     TDTKAPNSARTSSPVADESTWRPRRNPNFAVSSARGQPATEPAQEPEEAEPRVASVRL
                     TFDRLADPAATQLPAFEAQAARWEELRWSLERIRHRFGEGRLWRAVVDRPMAALPEHR
                     SRLVDIGG"
BASE COUNT          378 a         1142 c         1128 g          437 t
ORIGIN      
        1 atgcgaacgc ggtgctctcc cagctgagct acatccccgg gccgcgggga gtatagcaaa
       61 ggccaagcgg gccttcccca actagaacat ctgttctatc atcggttgtc cgatgaggac
      121 cgatgccgcc gccgtctcgc catccctggc gagcgccctc gagacgctcc gcgcacgcgg
      181 gctcgacctg cgtcgcggca ctcagccgat gcagcaggcg cagccacggc cctcgatccc
      241 gagcggccac ggggcgctcg atgtggctct cgccaccggc ggctggccgc gcggcgccct
      301 cgccctgctc gatgcgccag cgggcggggg agcgacgacg ctcgcgctcg gcagcctcgc
      361 cgcggcccag gccggtgggg gcctcgcggc ctggctcgac gtcgatggca ccttcgaccc
      421 cgccgtcgcg gcgcgcctcg gcgtgcagct cgactggctg ctgatcgccc accccaccga
      481 tgcgggcgag gccgtcgagc tggccgcctg gctcggacgc tcggggctgc tggacgccgt
      541 cgtcctcgac ctcggcgagc ggtcggggag cgtcgaccgg cgtgccctgg atcggctcgg
      601 cgcactcctc gcccgtgcgg gcgcgacgtc ggtgctgacc gttgcgccaa cggcccgtgc
      661 gacggtcggg gcggtggccg gcgtgcgggt cgcgctgcga cgcacggcgt ggctggcgct
      721 cgggcgcgac ctggtggggc aacgggtgca cgccaccgtc gagcggcacc gctgggcgct
      781 ggcgggcggg tccgcggagc tggacctgtg gttcgccgag ggtcgccgga tcgatgcgct
      841 ggccccgcgc ctggcgcagc cacagaaccc tgcgacgacc gaggagcgac cggcgctgcg
      901 ggtcgtgggc gcatgatcgg catcgggcct gaagacttgg aaggagacgg catgcggtac
      961 gcgatcagca tcccgaactt cggcgagtgg gccgacccgc gcacattcat cggtctcgca
     1021 caggatgccg aggccgccgg ctgggacggc ttcttcgcgt gggaccacat ccgcttcagc
     1081 gcgacgccac ttccggtcca cgacccgtgg gccctccttg cggggattgc cacggtgacg
     1141 gagcgcctgg tgatcggccc gatggtcacg ccgcttccgc gccgccggcc atgggtggtg
     1201 gcgcgccagg ccgtgaccat cgaccacctg tcgaacgggc ggatgatgct cggcgtcggc
     1261 atcggtgagc cggccgacat cgagctcgcc gccttcgggg aggcgaccga cctgaaggtc
     1321 cgcgccgaga tgctcgacga aggactggca attctcgacg gcctgtggag cggcgaggag
     1381 ttcagcttcg acgggaagca ctaccacctg gccccgatga ccttccttcc gcgtccgatc
     1441 cagcgcccgc ggatcccgat catcgtcgcc ggctactggc cgcaccgagg cccgattcgt
     1501 cgcgcggcgc gctgggacgg catgaacgcc ctcttcccgt acccgctcga gggctgggag
     1561 gagaaggcgc gtgccctcgt cgccgagctc cggtcgctgc gagcggaccc gaccggcccg
     1621 ttcgagtact tcgacggtgg ccacaccccg gacgacgggc cggacgccga tgacccggtc
     1681 ctttccgcgg acatcggtgc gacctggtgg atcgagagcc tcgatccgtg gcggttcacg
     1741 tgggacggtt ccgccgaggg ctggccgtcg gtcgagctcg cgcgggaccg catccggctc
     1801 ggtccgccgc ggtgacgtga tgccccgcct cctcgccctc gtctggccgg agctgcgtga
     1861 cccggacgcc gaggtcgacg cgcggccgtg ggaccgcatg ctcgacgcgc tcgatgacct
     1921 ctccccgcgg gtcgaggcgg tcgacctggg tgtcgccctc gtcgacatca ccggcctcga
     1981 gccgatgttc ggcccggaac gtcgggtcgc ggcacgcgcc gtgtcgctgg cccggaccgt
     2041 tgccccgctg cgcgtccgct gcgggatcgg cgagaaccgg tggctggcgt cgctggccgc
     2101 gcggctggcg cgcttcgagc gccccgaggc accggccgcg ctgtacatcc tcgaccgcgc
     2161 cgagctgccc gacctgccgc tctccctgct gaccgccgat ccgaaggtcc gccagcgctt
     2221 cgcgctgttc ggactgacga ccatggggca gctggccaac ctgccgcgct cggccgtcgg
     2281 cgcgcagctc gggccgaccg gggagcgtct tcaggcgctg gcccgcggcc acgacccccg
     2341 gccgctggtt ccgcggcggc gcccggaacg gctggaggcg cgcgacacgt tcgagccgcc
     2401 gatcgagggc gtgggcgggg tcgcgctgac gctgcgccgc ctggccgccg acctctgcga
     2461 ccggctgcgc acccggcacc tcgcgccggg tcgcgcgacc ctgaccctgg tcctcgagga
     2521 cgctccggcg ctgcgcgtcg cgcagccctt cccgcagccg gccctcgagc ccgattggat
     2581 cgcgcgcctc ctcctcagcc gcgtcgaggc ggccgcccgc ctccgtagtt gtcgtggagc
     2641 ggagctaacg gacacgaagg cgccgaattc ggcccgtacc tcgtcaccgg ttgcggacga
     2701 gagcacctgg cggccacgaa ggaaccccaa cttcgccgtt agttccgctc gggggcaacc
     2761 ggccacggaa cccgcccaag aaccggagga agctgagccg cgcgtcgcat cggtccggct
     2821 caccttcgat cggctggcgg atccggcggc gacccagctg cccgccttcg aggcccaggc
     2881 ggcgcgatgg gaggagctgc gctggtcgct ggagcgcatc cggcatcgat tcggggaggg
     2941 gcggctgtgg cgggccgtcg tggatcggcc gatggcggcg ctgccggagc atcggtcgcg
     3001 gctggtggac atcggcggat gacgcgctgc tacaccgacc acccgctggt ccaggtcgac
     3061 accgatgccg acggcgtccc cgttc
//
LOCUS       DASTYP010000215         5868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1222327, whole genome shotgun sequence.
ACCESSION   DASTYP010000215 DASTYP010000000
VERSION     DASTYP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222327"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFV59_09430"
     CDS             <1..691
                     /locus_tag="VFV59_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5326088.1"
                     /translation="SSFPSSASSASRRRYARWAHEPMSAGWVDELEPDAPDVVAVWRV
                     GDYSLAGLKAMRRELRARVGPGVAPRILAQQEAVANALESVLRSLSEDLLRAPGGEED
                     WNVAQAFAHTTAARRFLSTWAALDVDGSWPEHRPPAVAPSVPGRPDATRDELLVLLDK
                     SRRAVREAALKIEGHETNRCRMDHPLVGHLRCGEWLLFIGVHDLMHLEQLHAMADGSD
                     RGATAGPVGNA"
     gene            684..1820
                     /locus_tag="VFV59_09435"
     CDS             684..1820
                     /locus_tag="VFV59_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326089.1"
                     /translation="MPERPAPLVFVAAPREGQPFGSHAAIAADGQRLLATELSHRLGA
                     LGAAVLPIPAATSEDVPFHWGRWYVRAVRAALSASGEGQRVDAVGYAGAGSLALLSDG
                     VLDDLLSPIPGEVVANNRFSADAFVVAGDLARALEVLEACETDNAAPRRLSEAGFAWR
                     DLGAAPWSRFDVDTTLDLALLRLATRLDGMRPVHASVRAYLEMARLPGGGTLEVPHLE
                     ALGAVIRDRQAELVVAGRVAGATFAELETEAACRVRAFVEERGMRSARVAPHPPRSLL
                     ASLLERSSPAELVAELARMGDAVVLDTRVLMAALGGSSDASAWPPEEERLASDFGDWG
                     RVGTSWLRELTRAAIEGPVPVLLGGHALVSDGLRLILRAAWQGR"
     gene            2066..2425
                     /locus_tag="VFV59_09440"
     CDS             2066..2425
                     /locus_tag="VFV59_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5326090.1"
                     /translation="MDHTGLIYVIAFGAAATIFVFGTILFSWLITHRRRRPQKGLPYE
                     SGVDTIGDTWSRFGLSFYLYALLFVAFDVEIIFVYLWAITVRALEMQGIILVGIFLAI
                     LMFGELYAWKKGDLRWQ"
     gene            2530..3072
                     /locus_tag="VFV59_09445"
     CDS             2530..3072
                     /locus_tag="VFV59_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEU5326091.1"
                     /translation="MEEHPQGGFHGLLEVIPTKADYVLDLIRANSLWPLLSGLSCCAI
                     EMMSTATSVNDIDRFGMFPFRASPRQADVLIVAGTLTTKMAGPLVRLWEQMPEPKWVV
                     AMGTCTTSGGRFKRSYSVVQGVDRVMPVDVYVPGCPPRPEGLIYGMLKLQELVKQRRG
                     EWSTYVDRGVQRTRWEGDVR"
     gene            3012..3710
                     /locus_tag="VFV59_09450"
     CDS             3012..3710
                     /locus_tag="VFV59_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5326092.1"
                     /translation="MVDVRRSRRAADALGRRRPLTVPVAAGALVRLLDERLGSDLREL
                     WTDEDTVEFQVDGARAVDVLRTLRDDPDLGFFFLADAAAVDYGPKAHFEVVYHLYSDK
                     HPAWVRVRARVDRADPRIPSVTGLWEGAMFHEREMYDMMGIVFEGNRDLRRIYMSPDY
                     RSFPQRRDFVLPDDAADSPAAGVIPLERPETWDLARFRDAPAVTFPPSSEREEAPAES
                     VEGGETLADKSRPR"
     gene            3707..4993
                     /locus_tag="VFV59_09455"
     CDS             3707..4993
                     /locus_tag="VFV59_09455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU5326093.1"
                     /translation="MTRIDPDIQIPDPIDLDRQEEERLRRVAGLAPAPRTEGAEYAGF
                     EIVGIPDRPPQTEREREMYSLDEGEMLVNMGPQHPSTHGVMRLVVKVRGEIVVDIDPV
                     LGYLHRGIEKLCEHATYTTVLTYVDPMDYLSAMHNEHAPAIAFEKLYDIRAPKRAEYI
                     RALVVELNRVFSHGLMMGFLALDMGGLTPILYSFINRDEVVDLLSAISGQRMLFNFFR
                     VGGVNWDVNDEFLSRLGTWRSNVLKNIEMNERMITENEIFTARTKGMGVLTGQEAIDL
                     GVSGPNLRASGVPFDVRKAHPYSIYHELEFDVITQQSGDSYARYLQRIAEIKQSIHLI
                     DQIIDRMPHGPVQAQVPGIIRPKPGRAFGAVESPRGLYSVYAISDGGPTPYRFRMRDP
                     SFISLQAIPKLMPGRLIADTMAIMASFDPVMGGVDK"
     gene            4997..>5868
                     /locus_tag="VFV59_09460"
     CDS             4997..>5868
                     /locus_tag="VFV59_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEU5326094.1"
                     /translation="MDFELLLGIGRFLLATTLLLLLTVPTAFIIIQMEMKIIAQLALR
                     YGPNRIGPKGIFQSTIHGFKVLAKEDTVPDQADRVAFTMAPWMVYMAAAMSMLVIPFA
                     PGAIAADFNIGIVYFFAVLGLSVVGLLIAGWASYNKYSLLGGLRSAAQMVSYEIPLTL
                     SIVGAVVLAGTLNFGELIAWQRDHGWLLVWQPIGLGIFYISSLAEVNRTPFDMVEADS
                     ELVAGPFTEYSGMRFGFFFFAEYVALFIMSGILVSLFFGGWLAPWPFPAELGGFAGTL
                     YGIFWFFLKTYIFVA"
BASE COUNT          836 a         2081 c         2023 g          928 t
ORIGIN      
        1 ttcgtccttc ccgtcgagcg cttcgagcgc ctctaggcgc cgatacgccc gctgggcgca
       61 cgagccgatg agtgccggct gggtcgacga gctcgagccc gatgcgccgg acgtcgtcgc
      121 cgtctggcgg gtgggtgact actccctcgc cggcctgaag gccatgcgcc gcgagctccg
      181 cgcccgcgtc gggccggggg tggcgccgcg gatcctcgcg cagcaggagg cggtcgcgaa
      241 cgcgctcgag tcggtgctgc gctcgctttc agaggacctc ctgcgcgcgc cgggcggcga
      301 ggaggactgg aacgtggcgc aggccttcgc ccacaccacc gcagcgcgac ggttcctctc
      361 gacctgggcg gcgctcgacg tcgacgggtc gtggcccgag caccggccgc ctgccgtcgc
      421 gccgtccgtg cccgggcggc cggatgcgac gcgtgacgag ctgctggtcc tcctcgacaa
      481 gtcgcgacgg gccgtccgcg aggcggcact gaagatcgag gggcacgaga cgaaccgatg
      541 ccggatggac cacccgctcg tcggccacct gcgctgcggg gagtggctgc tgttcatcgg
      601 cgtgcacgac ctgatgcacc tcgagcagct gcacgcgatg gccgacggct cggaccgtgg
      661 agcaaccgcc ggaccggttg gcaatgcctg agcggccggc gccgctcgtc ttcgtggcgg
      721 ccccgcgcga ggggcagccg ttcggatccc acgccgcgat cgcggcggac ggacagcgcc
      781 tgctggcgac ggagctgagc catcgactcg gggcgctggg tgcggccgtc ctgccgatcc
      841 cggccgcgac gagcgaggac gtgccgttcc actggggccg gtggtacgtg cgcgctgtgc
      901 gcgcggccct ctccgccagc ggcgagggtc agcgagtgga cgccgtcggg tacgccggtg
      961 ccgggtcgct ggcgcttctc tcggacggcg tcctggacga cctgctgtcg ccgatcccgg
     1021 gtgaggtggt cgcgaacaac cggttcagcg ccgacgcgtt cgtggtcgcc ggggacctgg
     1081 cgcgcgcact cgaggtgctg gaggcctgcg agacggacaa cgcggcgccg cggcggctga
     1141 gcgaggccgg gttcgcgtgg cgcgatctgg gcgcggcacc ctggtcccgc ttcgacgtcg
     1201 acacgacgct ggacctggcg cttctgcgcc tcgcgacgcg gctcgacggc atgcggccgg
     1261 tgcatgcctc cgtccgcgcg tacctcgaga tggcccggct gccgggaggc ggcacgctcg
     1321 aggtgccgca cctggaggcc ctgggcgccg tcatccgcga ccgccaggcg gagctggtgg
     1381 tcgccggacg cgtggccggg gccaccttcg ccgagctcga gaccgaggcc gcctgccggg
     1441 tccgtgcgtt cgtcgaggag cgaggcatgc gatccgcgcg cgtggctccg catccgccgc
     1501 gctcgctgct ggcgtccctg ctcgagcgct cctcgccggc ggagctcgtc gctgagctgg
     1561 cccgcatggg cgacgccgtc gtgctcgaca cgcgggtgct gatggccgcc ctcggcggca
     1621 gctccgatgc ctccgcctgg ccgccggagg aggagcgtct ggcgtcggac ttcggcgatt
     1681 gggggcgggt cgggacgtcg tggctgcgcg agttgacgcg ggccgccatc gagggcccgg
     1741 tgccggtgct gctgggcggg cacgccctgg tcagcgacgg actgcggctc atcctccgcg
     1801 ccgcctggca ggggcgctag cggtccgagg gcgagggcgc ggcgtacgcg catcggcacg
     1861 gccggtggat cggccgcgga gcggctgctg ccgccttccc ctccgaccac gctcaacgga
     1921 gcggctgcta gactgtctca tcgaggcgag cgcctcgtgc gcgaccctca cccgacgaac
     1981 ccaaacgcgg gccccaccag gcgcccgatc gcaacgctac gcaacgcaaa cgcaccagca
     2041 caccgcgcgt gcgagctgag gtgagatgga tcacaccggg ctcatctacg tcatcgcgtt
     2101 cggggcggcc gcgacgatct tcgtcttcgg cacgatcctg ttcagctggc tgatcacgca
     2161 tcggcggcgg cgccctcaga agggcctgcc gtacgagtcg ggcgtggaca ccatcggtga
     2221 cacctggagc cggttcggcc tctcgttcta cctctacgcc ctgctcttcg tggccttcga
     2281 cgtcgagatc atcttcgtct acctgtgggc catcacggtc cgggccctcg agatgcaggg
     2341 catcatcctg gtcggcatct tccttgcgat cctgatgttc ggcgagctgt acgcgtggaa
     2401 gaagggtgat ctccgatggc agtagaacga ccgaacaccg cactcgacgc cggcggcgcg
     2461 ggcaccggcc aacccccgat catcattccc gaccaggccg gtgacgacga gctcctgcgt
     2521 gagttccgga tggaggagca cccgcagggc ggcttccacg gcctgctcga ggtcatcccg
     2581 accaaggccg actacgtcct ggacctgatc cgggccaact cgctgtggcc gctcctctcg
     2641 ggcctctcgt gctgtgccat cgagatgatg agcaccgcca ccagcgtcaa cgacatcgac
     2701 cgcttcggga tgttcccctt ccgcgccagc ccgcgccagg cggacgtgct gatcgtggcg
     2761 ggcacgctga ccaccaagat ggcagggccc ctggtccgcc tctgggagca gatgcccgag
     2821 ccgaagtggg tcgtggccat gggcacctgc accacgtcgg gcggccgctt caagcgctcg
     2881 tacagcgtgg tccagggcgt cgaccgcgtc atgcccgtcg acgtctacgt gccgggctgt
     2941 ccgccgcgtc ccgagggcct gatctacggc atgctcaagc tgcaggagct ggtgaagcag
     3001 cggcgcggcg agtggtcgac gtacgtcgat cgcggcgtgc agcggacgcg ctgggaagga
     3061 gacgtccgct gaccgtcccc gtcgccgccg gggcgctcgt ccgcctgctg gacgagcgcc
     3121 tgggttccga cctccgcgag ctgtggaccg acgaggacac cgtcgagttc caggtcgacg
     3181 gtgcccgcgc cgtcgacgtg ctccgcacgc tgcgcgacga ccccgacctc ggcttcttct
     3241 tcctggccga tgcggccgcc gtggactacg gccccaaggc gcacttcgag gtcgtctacc
     3301 acctgtacag cgacaagcac ccggcctggg tccgcgtccg cgcccgcgtc gaccgcgccg
     3361 atccccgcat cccgagcgtg accgggctgt gggagggagc gatgttccac gagcgcgaga
     3421 tgtacgacat gatgggcatc gtgttcgagg gcaaccgcga cctgcgccgc atctacatga
     3481 gccccgacta ccgcagcttc ccgcagcgca gggacttcgt cctgcccgac gacgccgccg
     3541 actcgccggc cgccggcgtg atcccgctgg agcgacccga gacctgggac ctggcccgct
     3601 tccgcgatgc cccggccgtc accttcccgc cctcctcgga gcgggaggag gcaccggccg
     3661 agagcgtgga gggcggcgag accctcgccg acaagagccg accgcgatga ccaggatcga
     3721 tcccgacatc cagatccccg acccgatcga cctcgaccgg caggaggagg agcgcctgcg
     3781 gcgcgtcgcc ggcctggcgc ccgcgccccg caccgagggc gccgagtacg ccggcttcga
     3841 gatcgtcggc atcccggacc gaccgcccca gaccgagcgc gaacgcgaga tgtactcgct
     3901 cgacgagggc gagatgctcg tcaacatggg gccgcagcac ccctcgaccc acggcgtgat
     3961 gcgcctggtg gtcaaggtgc gcggcgagat cgtggtggac atcgacccgg tgctcggcta
     4021 cctgcaccgc ggcatcgaga agctgtgcga gcacgcgact tacaccacgg tcctcaccta
     4081 cgtcgacccg atggactacc tgagcgcgat gcacaacgag cacgcgccgg ccatcgcctt
     4141 cgagaagctg tacgacatcc gggcgccgaa gcgggccgag tacatccgcg cgctggtggt
     4201 ggagctgaac cgggtgttca gccacggcct gatgatgggc ttcctggcgc tcgacatggg
     4261 tggcctgacg ccgatcctgt acagcttcat caaccgcgac gaggtcgtcg atctgctctc
     4321 tgccatcagc ggtcagcgca tgctgttcaa cttcttccgc gtcggcggcg tgaactggga
     4381 cgtcaacgac gagttcctct cacggctcgg cacgtggcgc tcgaacgtgc tgaagaacat
     4441 cgagatgaac gagcggatga tcaccgagaa cgagatcttc accgctcgca ccaagggcat
     4501 gggcgtgctc accgggcagg aggcgatcga cctcggcgtc tccggcccga acctgcgcgc
     4561 ttccggggtg cccttcgacg tccgcaaggc gcacccgtac tcgatctacc acgagctgga
     4621 gttcgacgtc atcacccagc agtcgggcga ctcgtacgcg cggtacctgc agcgcatcgc
     4681 cgagatcaag cagagcatcc acctgatcga ccagatcatc gacaggatgc cgcacgggcc
     4741 ggtgcaggcc caggtgcccg gcatcatccg gccgaagccg ggacgagcgt tcggggcggt
     4801 cgagtcgccg cgtggcctct acagcgtcta cgccatcagc gacggcggcc ctaccccgta
     4861 tcggttccgc atgcgcgacc ccagcttcat cagcctgcag gccattccga agctcatgcc
     4921 cggccgcctc atcgccgata cgatggcgat catggcgagc ttcgacccgg tgatgggcgg
     4981 cgtggacaag tagccgatgg acttcgagct gctgcttggc atcggtcggt tcctgctggc
     5041 cacgaccctg ctcctgctgc tgaccgtccc gaccgccttc atcatcatcc agatggagat
     5101 gaagatcatc gcgcagctgg cgctgcgcta cgggccgaac cgcatcgggc cgaagggcat
     5161 cttccagtcc acgattcacg gcttcaaggt gctggccaag gaggacacgg tccccgacca
     5221 ggccgatcgc gtggcgttca ccatggcccc gtggatggtc tacatggcgg cggccatgag
     5281 catgctggtc atcccgttcg cgccgggcgc catcgccgcg gacttcaaca tcggcatcgt
     5341 gtacttcttc gcggtgctcg gcctctcggt ggtcggcctg ctcatcgccg gctgggcgag
     5401 ctacaacaag tactcgctgc tcggcggcct gcgctccgcc gcgcagatgg tgagctacga
     5461 gatcccgctg acgctctcga tcgtcggcgc cgtcgtgctg gccggcacgc tgaacttcgg
     5521 cgagctgatc gcctggcagc gtgaccacgg atggctgctg gtctggcagc ccatcggcct
     5581 gggcatcttc tacatcagct cgctggccga ggtgaaccgc acgccgttcg acatggtcga
     5641 ggccgactcg gagctggtgg ccggcccgtt caccgagtac tcgggcatgc gcttcggctt
     5701 cttcttcttc gccgagtacg tggcgctgtt catcatgagc ggcatcctgg tcagcctctt
     5761 cttcggcggc tggctggccc cctggccgtt ccccgcggag ctcggcggct tcgccggcac
     5821 gctgtacggc atcttctggt tcttcctgaa gacctacatc ttcgtggc
//
LOCUS       DASTYP010000216         6666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_128789, whole genome shotgun sequence.
ACCESSION   DASTYP010000216 DASTYP010000000
VERSION     DASTYP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128789"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..398)
                     /locus_tag="VFV59_09465"
     CDS             complement(183..398)
                     /locus_tag="VFV59_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEU5326095.1"
                     /translation="MSPELGRVLLVIGIVLVVVGGLAMAGVRLPFGRLPGDIAIEGER
                     GGFYFPIVTMLVISIVLTVLVNLFLRR"
     gene            complement(403..1461)
                     /gene="ruvB"
                     /locus_tag="VFV59_09470"
     CDS             complement(403..1461)
                     /gene="ruvB"
                     /locus_tag="VFV59_09470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5326096.1"
                     /translation="MTIERITSAEIAEEELALESTVRPRRLDEFAGQDRVKENLAILI
                     DAAQHRSEPVDHVLLYGPPGLGKTTLANIVAAEMGAQIRTTSGPAIERAGDLAAVLTS
                     LGKGDVLFIDEIHRLSHAVEEILYPAMEDFALDVVLGKGPGARTVRLQIEHVTVIGAT
                     TRVGSITGPLRDRFGATYRLDYYTQGELERILHRSADILGISLTDAAATLVAGRSRGT
                     PRIANRLLKRVRDYAQVRGGGDGLVTDALAADALGLLEIDERGLDAMDRQLLRAMAEH
                     HAGGPVGLATMAATVSEPIETIEDVVEPYLMQIGLLARTSRGRQLTPSGWEHLKLTPP
                     PNSAAASVQAQQPGLFDG"
     gene            complement(1501..2547)
                     /locus_tag="VFV59_09475"
     CDS             complement(1501..2547)
                     /locus_tag="VFV59_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEU5326097.1"
                     /translation="MQVAAAVLAAGASTRFGAPKHEVLLAGEPMYVMAARTAHAAGLE
                     PVLVVVPSETVVPPELVPVVNDDPDAGLSRSVQLGVAAVPPECDAVVLLLGDEPTISG
                     DDLAAVVAAATGERSVVATRAGDRIGPPVLLMRDAFDLVEGLRGDEGLRSLLRGDPAE
                     VTVVERPVPLADIDTPADLEALAPRCPGCGVRVPGPADGPTHEYIESSPGCWAVFNEL
                     LAREFGDASYGRVHRHTVDVYAVQHPGTNDRRQRQSVALHLIGLCHWLEHDLEQDRLN
                     ATTQRLATQDGDWPWLEPPSTYDMTVVDVLRARSGAEHRELARAWAQSTWDAWAEHHA
                     QVRAWAADALHHAG"
     gene            2595..3257
                     /locus_tag="VFV59_09480"
     CDS             2595..3257
                     /locus_tag="VFV59_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326098.1"
                     /translation="MRRLLGSSLALLLVACGGPSPSPGGPTATESADAASPGASSEGD
                     DVAELPAPGGEIPPGTYTRSAFAPRVTFELGPGWTARQLAAGFFDVEQDAGSVDVIAV
                     QFGQVVGHDRAQAAATAIAENVNLVVQSGPEEVALGGNSGIQVTVDTVDPPDTQPPVF
                     RQVVSVAAGPLSIASGRRLQLTFVDTDAGLLAVLVGGSIRNWDATLEAVTPVLDSLTI
                     EG"
     gene            3282..4880
                     /locus_tag="VFV59_09485"
     CDS             3282..4880
                     /locus_tag="VFV59_09485"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU5326099.1"
                     /translation="MSARYDLLIENGTVVNGSGERGFAAAVGVRHGRLEVVYGDTSAL
                     QADERIDASGQVVAPGFVDLHSHSGLTLFTAPTHEPKVLQGVTTEVIGVDGLSYAPFR
                     DAGDLRALVEMNAGLDGRPDLAYDWSTVTSYLDRLDALHPSVNLAFLVGNTALRVAAV
                     GWDELEATPAQVADMRAMLREAMEEGAIGLSSGLDYPPGSYATTDELAELTNEAGRHG
                     GFYHTHVRYPLGDRYLDPYREAIEIGRRGEGPAHITHFYHKQIFPEGPGPMLALVDDA
                     RTEGLDVTFDTYPYEWASTRLLIMIPPWAQSGGPMRTKERLADRSVRDRIRRELAERG
                     VLFAGRRAWDDVRLGAFTRPDLLRWEGRTLGDVIADRGDDPVDVLCDLLLAEDLRLNQ
                     VTPGPWSESLREFVRHPVGMIGTDSTFVGAKPSPRSYGSFPRVLGQFVREEALLSLEE
                     AVRSMTSAPAQRIGLSDRGVIRDGAVADLVVLDPDAVRATCTYDEPCSYPEGIGWVIV
                     NGDVVVRDGAHTGARPGRVLRRGG"
     gene            complement(4889..5338)
                     /locus_tag="VFV59_09490"
     CDS             complement(4889..5338)
                     /locus_tag="VFV59_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326100.1"
                     /translation="MSFFEKARQTASQGLSQLTGAESEGESRVTAESVTASVRELAGH
                     AKRGVVTVIEKIDPDTLADLIIKATAIQEKANAALARKKAAYRIGDLSITATIPPQIG
                     FSIARIGDIEEAIDETALLESSQLEDRIPTADEAVISLDGETVPTEA"
     gene            5397..>6666
                     /locus_tag="VFV59_09495"
     CDS             5397..>6666
                     /locus_tag="VFV59_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEU5326101.1"
                     /translation="MERPSLTVRTPAPGVANPDMPAVLTGTAPVVGRARPAAPLRREG
                     PAKVTGEAKYADDLVVPGAWYGATIRSTEPHARLLGLELDDAFDWKRVVVVTADDIPG
                     ENIVSLIDDDQPVLVPIGGEIRHQAEPCALIAAPDRATLREARRHVSLRTERLPAVFD
                     PLESDIEFAHYTVAQGDPEAAMAGADVVIEGTYRVGHQEQLYIENQAMIAVPRDDGGM
                     TVHGSLQCPYYIHKAMKRALNMGDELAVVVQAETGGGFGGKEEYPSIIALHASLLALK
                     CRKPVRMIYDRHEDISATTKRHPAVIRSRWGVGSDGELLAQEIEVIMDGGAYCTLTPV
                     VLSRGILHAGGPYRCPNVRITGRAMATNTPPNGAFRGFGAPQTEFAAEMQINRIAEAL
                     DASPLDLRRRWVYREGDTTATGQVLRESVGG"
BASE COUNT          954 a         2387 c         2368 g          957 t
ORIGIN      
        1 gtggcgctgc cgcgctcgac ctcggtacct ccgttgcctc tgcctccact cgggtcaccc
       61 tccgcgtctc cactgggcac ctggagcgca cgacaccgcg ccgggcactg gtgccgccgc
      121 gcagcctacg ccgatgcgcg cctcggtgag gcgcgttttc gcgcacgcgc gcggagagcc
      181 gatcagcgcc gcaggaacag gttgaccagc acggtcagca cgatgctgat gaccagcatc
      241 gtgacgatcg ggaagtagaa gccgccccgc tcgccctcga tggcgatgtc ccccggcagc
      301 cgaccgaacg gaagccggac gcccgccatc gccagcccgc cgacgaccac cagcacgatg
      361 ccgatcacca gcaggacgcg gccgagctcc ggggacaccg gatcagccgt cgaagaggcc
      421 gggctgctgc gcctggaccg atgccgccgc cgagttcggg ggcggcgtga gcttgaggtg
      481 ctcccagccc gacggcgtca gctggcggcc ccgggaggtt cgagccagca agccgatctg
      541 catgaggtat ggctcgacca cgtcctcgat cgtctcgatc ggctcactga cggtggccgc
      601 catggtggcg aggcccaccg ggccaccggc atggtgctcg gccatggcgc gaagcagctg
      661 gcggtccatg gcgtccaacc cgcgctcgtc gatctcgagc aggccgagcg catcggccgc
      721 cagcgcgtcg gtcacgaggc cgtctccccc accccgaacc tgcgcgtagt cgcgcacccg
      781 cttgagcagc cggttggcga tgcgaggggt gccgcgcgag cggccggcca caagcgtggc
      841 ggccgcatcg gtcagggaga tgccgaggat gtcggccgag cggtggagga ttcgctccag
      901 ctcgccctgc gtgtagtagt cgagccggta cgtggcgccg aaccggtcgc gcaacgggcc
      961 ggtgatgctg ccgacgcgcg tcgtcgcccc gatgacggtg acgtgctcga tctggagccg
     1021 caccgtgcgc gcgcccggcc ccttgccgag cacgacgtcg agggcgaagt cctccatggc
     1081 cgggtagaga atctcctcga cggcgtggct cagccggtgg atctcgtcga tgaacagcac
     1141 atcgcccttg cccaggctcg tgagcacggc ggcgaggtcg ccggcgcgct cgatcgcggg
     1201 gccggacgtg gtacggatct gcgcgcccat ctcggcggcc acgatgttgg ccagggtcgt
     1261 cttgccgagc cccggcgggc cgtagagcag gacgtgatcg accggctcgg accgatgctg
     1321 cgccgcgtcg atcaggatcg ccaggttctc cttcacgcgg tcctggccgg cgaactcgtc
     1381 caggcgccgc ggacggacgg tcgactcgag ggcaagctcc tcctcggcga tctcggcgct
     1441 ggtgatgcgc tcgatcgtca tcggtccgcg tccgtcgccg aatcgaacat gtgagcgatt
     1501 ctaccccgcg tggtgcagcg catcggccgc ccaggcgcgg acctgcgcgt ggtgttcggc
     1561 ccacgcgtcc caggtcgact gcgcccaggc tcgcgccagc tcccggtgct ccgcgccgct
     1621 gcgcgcccga agcacgtcca ccaccgtcat gtcgtacgtc gacggcggct cgagccaggg
     1681 ccagtcccca tcctgggtcg cgagccgttg ggtggtggcg ttgagccgat cctgttcgag
     1741 gtcgtgctcc agccagtggc acagaccgat caggtgcaga gcgaccgact gccgctgccg
     1801 ccggtcgttc gtcccgggat gctgcaccgc gtagacgtcg acggtgtgcc ggtgcacccg
     1861 cccgtacgac gcgtcgccga actcgcgagc gagcagctca ttgaagactg cccagcagcc
     1921 gggcgacgac tcgatgtact cgtgcgtcgg cccgtcggcc ggccccggca cgcgaacgcc
     1981 gcagccggga cagcgcggcg caagcgcttc caggtcggcc ggggtgtcga tgtccgcgag
     2041 cggcacgggc cgctcgacca ccgtcacctc agcgggatcc ccccggagga gcgaccgaag
     2101 accctcgtcg cctcggaggc cctcgacaag gtcgaaggca tcccgcatga ggagcaccgg
     2161 tggaccgatg cggtcgccgg cgcgcgtcgc cacgacggac cgctcccccg tcgccgcggc
     2221 cacgactgcg gcgagatcgt ctcccgagat ggtgggctca tcgccgagca ggaggaccac
     2281 cgcgtcacac tccggcggca ccgccgccac gccgagctgc acggagcggc tgagtcccgc
     2341 atcggggtcg tcgttcacca ccggaacgag ctccggcggc accaccgttt ccgacggcac
     2401 gacgacaagg accggctcca gtcccgccgc gtgcgcggtg cgggcggcca tgacgtacat
     2461 cggctcgccg gccagcagca cctcgtgctt cggggcgccg aagcgggtcg aggcaccggc
     2521 ggcgagcacc gcggcggcaa cctgcatcgg ctcattctga accccgagcc gagcggccga
     2581 gtatggtcag gcgaatgcgt cgcctgctcg ggtccagcct cgcgctcctg ctggtcgcct
     2641 gtggcggacc gagcccgtcc cctggcggcc cgactgccac ggagagcgcg gatgccgcgt
     2701 cgcccggagc gtcgagcgag ggtgatgacg tggccgagct tcccgctcca ggcggcgaga
     2761 tcccgcccgg gacgtacacg cggtcggcgt tcgcgcctcg cgtgaccttc gagctcggac
     2821 cgggatggac cgcccggcag ctggccgccg gcttcttcga tgtcgagcag gacgctggct
     2881 cggtggacgt catcgcggtc cagttcggcc aggtggttgg ccacgaccgc gcccaggcgg
     2941 cggcgaccgc gatcgccgag aacgtcaacc tcgtggtgca gtcaggaccg gaagaggtcg
     3001 ccctcggcgg caactctggc atccaggtga ccgtcgacac cgtcgatcca ccagacacgc
     3061 agccgccggt cttccgccag gtcgtcagcg tcgccgccgg cccgctgtcg atcgcatccg
     3121 gacgtcgcct gcagctcacc ttcgtcgaca ccgatgccgg tctgctcgcg gtcctggtcg
     3181 gcggctcgat ccgcaactgg gatgcgacgc tggaggcggt caccccggtg ctggactcgc
     3241 tgacgatcga gggctgacgg cggcgcgtac catgcgctgc catgtccgct cgctacgacc
     3301 tgctcatcga gaacgggacc gtcgtcaacg ggagcggcga acgggggttc gccgccgcgg
     3361 tgggcgtccg tcacgggcgc ctcgaggtcg tctacgggga tacgagcgcg ctccaagcgg
     3421 atgagcgcat cgacgcgagc ggccaggtcg tggctcccgg attcgtggac ctgcactcgc
     3481 actcggggct gacgctgttc acggcaccga cccacgagcc gaaggtgttg cagggcgtga
     3541 ccaccgaggt catcggcgtc gacggcctgt cctacgcgcc cttccgggat gccggcgacc
     3601 tccgagccct ggtcgagatg aacgcggggc tcgacgggcg acccgacctc gcctacgact
     3661 ggtcgaccgt cacctcgtac ctggatcggc tcgacgcgct ccatccatcg gtcaacctgg
     3721 cgttcctggt cgggaacacc gcgctgcgcg tcgcggccgt cggctgggac gaattggagg
     3781 caacgccggc gcaggtggcg gacatgcgcg ccatgctccg ggaggcgatg gaggaggggg
     3841 ctatcggcct ctcatccggc ctcgactacc cgccgggctc ctacgcgacc accgacgaac
     3901 tcgccgagct gacgaacgag gcggggcgcc acggcggctt ctaccacacg cacgtgcgct
     3961 atccgctggg cgaccggtac ctcgacccgt accgggaggc gatcgagatc gggcgacgcg
     4021 gcgaggggcc cgcccacatc acccacttct atcacaagca gatcttcccg gaaggtccgg
     4081 ggccgatgct ggcgctggtc gacgacgcgc gcacagaggg cctggacgtg accttcgaca
     4141 cctacccgta cgagtgggcc agcacccggc tgctgatcat gatcccgccc tgggcgcagt
     4201 cgggtggacc gatgcggacg aaggagcggc tggccgaccg ctcggtgcgc gaccgcatcc
     4261 gccgagagct ggcggaacgg ggggtcctct tcgccggtcg tcgggcgtgg gacgatgtgc
     4321 gcctgggcgc cttcacgcgc cccgacctgc tgcgctggga gggacgcacg ctgggcgacg
     4381 tgatcgcgga ccggggcgac gacccggtcg acgtcctgtg cgatctgctg ctggcggagg
     4441 acctgcgcct caaccaggtg acgcccggcc cgtggagcga gtcgctgcgc gagttcgtcc
     4501 gccacccggt cggcatgatc ggcacggact cgacgttcgt cggcgccaag ccctcgccgc
     4561 gcagctacgg ctcgttcccg cgcgtcctgg ggcagttcgt gcgcgaagag gcgctgctca
     4621 gcctggagga ggcggtgcga tcgatgacgt ccgcgcccgc gcagcgcatc ggcctctccg
     4681 accggggggt catccgcgac ggggccgtcg ccgacctcgt cgtcttggat ccggatgccg
     4741 tccgggccac gtgcacgtac gacgagccgt gctcgtaccc cgaggggatc ggctgggtga
     4801 tcgtcaacgg cgacgtggtg gtgcgcgacg gcgcgcacac cggcgcgcgt cccggtcgag
     4861 tcctgcgccg cggcggctga gcgctgcgtc aggcctcggt ggggaccgtc tcgccatcca
     4921 gggagatgac ggcctcgtcg gcggtcggga tccggtcctc gagctggctc gactcgagca
     4981 gcgccgtctc atcgatcgcc tcctcgatgt cgccgatgcg cgcgatcgag aagccgatct
     5041 ggggcgggat cgtggccgtg atgctcaggt cgccgatccg gtaggccgcc ttcttgcggg
     5101 ccagcgcggc gttcgccttc tcctggatgg cggtcgcctt gatgatcagg tccgcgagcg
     5161 tgtcgggatc gatcttctcg atgacggtca ccacgccgcg cttggcgtgg cccgccagct
     5221 cgcgcacgga cgccgtgacc gattcggcgg tcacgcgact ttcgccctcg ctctcggcgc
     5281 cggtgagctg gctgaggccc tgggacgcgg tctgtcgcgc cttctcgaag aagctcatcg
     5341 gcgcaactgt acgcagcacg tcaatcgccg caggtgcggc gcgctagcat gagccgatgg
     5401 agcgcccgag cctcaccgtg cgcacgcctg ctcccggcgt cgcgaacccc gatatgcccg
     5461 ctgtcctgac cggcacggct cccgtcgtcg ggcgcgcgcg acccgcggct ccgctgcgcc
     5521 gtgagggccc ggccaaggtc accggcgagg ccaagtacgc cgacgatctc gtcgtcccgg
     5581 gggcctggta tggcgcgacg atccgatcga ccgagcccca tgcacggctg ctgggcctcg
     5641 agctggatga tgcgttcgac tggaagcggg tcgtcgtggt caccgccgac gacatccccg
     5701 gcgagaacat cgtgagcctc atcgacgacg accagcccgt gctcgtgccg atcggcggcg
     5761 agatccgcca ccaggcggag ccgtgcgcgc tgatcgcggc gccggaccgc gcgaccctcc
     5821 gcgaggcgcg acgtcacgtg tcgctgcgca ccgagcggct ccccgccgtc ttcgacccgc
     5881 tcgagtccga catcgagttc gcgcactaca ccgtggccca gggcgatccc gaggcagcca
     5941 tggccggggc ggacgtcgtc atcgagggga cgtaccgcgt gggccaccag gagcagctgt
     6001 acatcgagaa ccaggcgatg atcgccgtgc cgcgcgacga cggcggcatg accgtccacg
     6061 ggtcgctgca gtgcccgtac tacatccaca aggcgatgaa gcgggcgctg aacatgggcg
     6121 acgagctggc ggtcgtggtc caggcggaga ccggtggcgg cttcggcggg aaggaggagt
     6181 acccgtcgat catcgcgctc cacgcctcgc tgctggccct gaagtgccgc aagccggtgc
     6241 ggatgatcta cgaccgccac gaggacatca gcgccaccac caagcggcac ccagcggtca
     6301 tccggtcgcg ctggggggtt ggctccgacg gcgagctgct ggcccaggag atcgaggtga
     6361 tcatggacgg cggcgcgtac tgcacgctca cgccggtcgt gctcagccgc ggcatccttc
     6421 acgccggcgg gccgtatcgc tgcccgaacg tccggatcac cgggcgtgcc atggcgacga
     6481 acacgccacc caacggcgcc ttccggggct tcggcgcgcc gcagaccgag ttcgctgccg
     6541 agatgcagat caaccgcatc gccgaggcgc tcgacgcctc cccgctcgac ctccggcgtc
     6601 ggtgggtgta ccgggagggc gacacgacgg cgaccggcca ggtgctgcgc gagtcggtcg
     6661 gaggca
//
LOCUS       DASTYP010000217         8240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1587069, whole genome shotgun sequence.
ACCESSION   DASTYP010000217 DASTYP010000000
VERSION     DASTYP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587069"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..546)
                     /locus_tag="VFV59_09500"
     CDS             complement(106..546)
                     /locus_tag="VFV59_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326102.1"
                     /translation="MRHRRLAALVLGLAVTGCATEAAETPEPRTTGTPVPTTEVRSTA
                     RSADAAAPPELAGTWRRVVQGEEVLLSLRDTGYRIQRAGEMGSGSITVEGEIVTFFAS
                     SRCDGSGRYTWAIVEDRLQLTEIDDPCSGRTDVLLFGTFGRVGE"
     gene            644..1414
                     /locus_tag="VFV59_09505"
     CDS             644..1414
                     /locus_tag="VFV59_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5326103.1"
                     /translation="MTAFLASTQATGDDALSPATLAGLCHVVGAEEAEYFELRRPDRA
                     VLALVESHEFESVPGTDEALAAYGHQNPVGWRRWGPADGPMRLSARVSRTALQRTEFY
                     NEGMRPNGLTDVLKVWLHRGAESIACVQLWLRGGTFTARQEDMLGVLQSHLIEMRQAP
                     SARAAPVGRVEGAMLTRREAEVLTWAVRGASDAEIASRLGLSVGTVGKHLEHAFAALG
                     VHSRAAALWQLQDLESRRSPASRHARPSVVGSPGDRPA"
     gene            1398..2828
                     /locus_tag="VFV59_09510"
     CDS             1398..2828
                     /locus_tag="VFV59_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5326104.1"
                     /translation="MTDLPDRPADTTLGELRAAGYRSRTVKEEMRANLLARLRRGDPF
                     LPGILGYEDTVLPQLENAILAGQDVILLGERGQAKSRIARSLVALLDPWLPYLAGTEL
                     HDDPLHPVSPLGRRIVEEEGDGASIGWWAAEDRYSEKLATPDISIADLIGEVDPVKVA
                     EGRYLSDELTIHYGLLPRTHRGVFSLNELPDLAERIQVGLLNVMEERDVQVRGYRVRL
                     DLDLFVIASANPEDYTSRGRIITPLKDRFGAQIRTHYPEETTTEIAIMEAEAARFDPE
                     GGDGGVPVEVPGFMKEVVAELSQLARRSSEVSQRSGVSVRVTIANHETLVAAALKRAV
                     RLDEASAAPRISDLGSVVASTAGKIELETLGDETREDRVVEKLVARAVVNVFNRHFAL
                     SELGPLLDRFEEESLSAEVGDLVPSSQHAALAGRVPELAAAVARLDAGESPAGVASAT
                     EFVLEGLHLNRRLNKERRAGGVRYAR"
     gene            2902..3540
                     /locus_tag="VFV59_09515"
     CDS             2902..3540
                     /locus_tag="VFV59_09515"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU5326105.1"
                     /translation="MTVLTQPRVSFETDDDLRRLASARRDRVRFVNVPAWRYLALDGT
                     EQPGSEAYQQAIGVLYGTAYRLHFALKARGVDAPVGGLEGLYWLTPEQLAAEGTTGSA
                     QDVEWRWRLIIAVPSEASEDEVDDAVRRGEPNDMLRRLYLDRWTEGPAAQILHVGPYD
                     AERPTLRRLEQAISAAGLRARGPHHEIYVNDPRRAGPERTKTVLRQGVEAAG"
     gene            complement(3608..3937)
                     /locus_tag="VFV59_09520"
     CDS             complement(3608..3937)
                     /locus_tag="VFV59_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326106.1"
                     /translation="MLDLLGWNVGMSGIAAIVLVTASILIGAIPQFIGEPRLAYEWVV
                     TAVGVLVGGWLGSEAFGAASTWGPVFDGLYIVPALIGGIVLGAIVDAVVRYVSGGSYT
                     HHAPRPI"
     gene            complement(4053..4979)
                     /locus_tag="VFV59_09525"
     CDS             complement(4053..4979)
                     /locus_tag="VFV59_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5326107.1"
                     /translation="MHPFRFAVHTGGGRLTHDFPALVRRTEELGYDALYVTDHLGRQL
                     SPIAALATAAALTSRIRIGAYVFANDFRHPLILAREAATLDVLSGGRLDLGLGAGWMR
                     SDYRRIGMPYDRPGLRIDRLEEALGLIKRLLSGEAVTHEGRFYRLEGARVGPLPVQRP
                     HPPIMLGGGGPRLLRMAAREAQIVGFAPQMGTAGRPMLRTTTESALADRVAVVRAAAG
                     PRFELLELNVFVADAGVVGDGQPLPASLSALVKSAGPAAVGGSPYLLYGTASQLRERL
                     LRRRERLGITAYGIPASAMEAFAPIVERLRDR"
     gene            complement(5004..5951)
                     /gene="arcC"
                     /locus_tag="VFV59_09530"
     CDS             complement(5004..5951)
                     /gene="arcC"
                     /locus_tag="VFV59_09530"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU5326108.1"
                     /translation="MSKSTLVVAIGGNSLIKDPQHMSVPDQYRALGETSGHIARIIKD
                     GWHVAIGHGNGPQVGFILRRSELSAHELHEVPLDVCDADTQGAIGYELQQNLGRDFRQ
                     LRIDRQAVTVVTQVEVDPADPAFANPSKPIGTFMDEAQARIREERDGWVVREDAGRGW
                     RRVVPSPRPIRIVEERAVKTLLREDFVVITVGGGGIPVVTNGQGDLEGVAAVIDKDFA
                     SALLATTLRADVFMISTAVDRVALDWGKPEQRWVDSLTLAEAKSYLAEGTHFASGSMA
                     PKIEACIEFLEHGGGEAIITSPDRMEAALAGEAGTRIVA"
     gene            complement(5958..6935)
                     /locus_tag="VFV59_09535"
     CDS             complement(5958..6935)
                     /locus_tag="VFV59_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5326109.1"
                     /translation="MQTGLRGRDLISLSELTLEEIETILDVAAQLKRERALGIGHPLL
                     RDKVLAMLFFFSSTRTRASFEAGMAQLGGHAQFIESRTTQIAHGDTAKEIGEILGRYN
                     DGIAIRHVDWNVGNEYIRGVAEASRVPVLNMQCDRFHPHQILADLLTMREKLGDLRGR
                     TLTLSWAYAASYQKPISVPQDLVTAATRFGMNVRLVHPPEFALEPDVVEEARLNARSS
                     GGSLELMEDFDAGFRDSDVIYAKSWGALLTAKDETEGAEIAQRYTDWITDERRIALAS
                     DHAIFMHPLPADRNVEVTDAVIDSPQSVVYDEAENRLHAQKAVMALTMR"
     gene            complement(6950..7903)
                     /gene="pyrB"
                     /locus_tag="VFV59_09540"
     CDS             complement(6950..7903)
                     /gene="pyrB"
                     /locus_tag="VFV59_09540"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEU5326110.1"
                     /translation="MRSFLGRDILGLKDFSREDFFRVFDAANELRPYAVNRRNGELLK
                     HKTLLTAFYQPSTRTRLSTEAAMHRLGGAVLGFSDAKMTRAGDFYQESMKDTILMLES
                     YGDVIAMRHYAEGAPAEAARYASVPIINCGDGWGQHPTQVLTDLYTILLERGTLDGLT
                     YLLIGDMRMRTMHSILHALSQFDAEAIVVSPPDMALKDEFKAELDAMGTVYREAPDVR
                     EVIGEADIIYLEPVVQADYTQSRVERSGEVGRTAAEYQVDAALMRSRAKRSSIVLHSL
                     PRMDELLPDVDETRHQRYWVEASNGVSLRMALLALVLGAME"
     gene            complement(7900..>8240)
                     /locus_tag="VFV59_09545"
     CDS             complement(7900..>8240)
                     /locus_tag="VFV59_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326111.1"
                     /translation="VLRRAGKHRSGYMWPWEHPEGSAAHGILDDETYDWLAVVRAMLL
                     TRGGPIVVDEATIANANALARTTTSIDVDPTGSAGLAGLIRLHRDGDLSPAETSLVLF
                     TGIRRTGDPR"
BASE COUNT         1186 a         2843 c         2981 g         1230 t
ORIGIN      
        1 gagctccagg agcgcggcac cttcctggag gagctggcgg agcgcgggat ctgcgatccc
       61 gcatcggtgg cgctggcggt ccgcgactac tccgcctgag cgcggctact cgcccacgcg
      121 gccgaacgtg ccgaagagca ggacgtcggt tcgtcccgaa cacggatcgt cgatctcggt
      181 gagctgaagc cggtcctcga cgatggccca ggtgtatcgg ccggacccgt cgcatcggct
      241 gctcgcgaag aaggtcacga tctcgccctc caccgtgatg ctgcccgagc ccatctcgcc
      301 cgcgcgctgg atccgatacc cggtgtcgcg caggctgagg aggacctcct cgccctggac
      361 gaccctccgc cacgtccccg ccagttccgg cggtgcggcg gcatccgcgg agcgcgccgt
      421 cgaccgcacc tcggtcgtcg gaacgggcgt cccggtcgtg cgaggttccg gggtctcggc
      481 ggcctcggtc gcgcatcccg tcacggcgag ccccagcacg agagcagcga gtcgacgatg
      541 ccgcatcacg acacgagcat gcccggcgcc aggccttgct gccatacggc gtgcggcgta
      601 gtttgtcgcg atgccgcgag ttacccagca ggacctggcc cggatgacgg cattcctggc
      661 gtcgacccag gcaaccggcg acgacgcgct gtcacccgcg acgctcgcgg gcctgtgtca
      721 cgtggtcggc gctgaggagg cagagtactt cgagctgcga cggccggatc gtgcggtgct
      781 cgccctggtg gagagccacg aattcgagtc ggtccccggc accgacgagg cgcttgcggc
      841 gtacgggcac cagaacccgg tggggtggcg ccgctggggt ccggcggatg gtccgatgcg
      901 cctctcggcc cgagtcagtc gtacggcgct gcaacggacc gagttctaca acgagggaat
      961 gcggcccaac ggcctcaccg atgtcctcaa ggtgtggctc caccgtggtg cggagtccat
     1021 cgcgtgcgtg cagctgtggc tgcggggcgg gacgttcacc gcgaggcagg aggacatgct
     1081 cggcgtcctc cagtcgcacc tcatcgagat gcgtcaggcg cccagcgcac gtgcggcgcc
     1141 ggtcgggcgc gtcgaaggcg cgatgctgac ccgtcgcgag gccgaggtgc tgacgtgggc
     1201 cgtccggggt gcgtcggacg ccgagatcgc ctcgcggctc gggctgtcgg tcgggaccgt
     1261 tggcaagcac ctcgagcacg ccttcgctgc gctcggcgtg cactcgcggg cggcggcact
     1321 gtggcaactg caggacctgg agtctcggcg gagtccggcc tcacggcacg cgcggccttc
     1381 ggtggtaggc tcacccggtg accgacctgc ctgatcgacc agccgatacc accctggggg
     1441 agttgcgcgc cgcggggtat cggtcacgga cggtcaagga ggagatgcgc gccaacctgc
     1501 tggcgcggct ccggcgcggc gatccgttcc tgccgggaat cctgggctac gaggacacgg
     1561 tcctgcccca gctggagaac gccatcctgg ccgggcagga cgtcatcctc ctcggcgagc
     1621 gtggccaggc caagagccgc atcgcgcgct cgttggtggc gctgctggac ccgtggctgc
     1681 cgtacctggc cgggacggag ctccacgacg atccgttgca cccggtctcg ccgctcggtc
     1741 ggcggatcgt cgaggaggag ggtgacggcg cgagcatcgg ctggtgggcc gcggaggatc
     1801 ggtacagcga gaagctggcg accccggaca tcagcatcgc cgacctcatc ggcgaggtgg
     1861 atccggtcaa ggtggccgag ggccgctacc tgtccgacga gctcaccatc cactacgggc
     1921 tgctgccgag gacgcatcgg ggcgtcttca gcctgaacga gctgccggac ctggccgagc
     1981 gcatccaggt cggcctgctc aacgtgatgg aggagcgcga cgtccaggtg cgcggctacc
     2041 gggtgcggct cgacctcgac ctgttcgtca tcgccagcgc caaccccgag gactacacca
     2101 gtcgcggtcg gatcatcacg cccctgaagg accgattcgg cgcccagatc cggacgcact
     2161 acccggagga gacgacgacc gagatcgcga tcatggaggc ggaggccgcg cgcttcgacc
     2221 cggagggcgg cgatggcggc gtgcccgtcg aggtgccggg cttcatgaag gaggtcgtcg
     2281 cggagctctc gcagctcgcg cggcgctcgt ccgaggtgtc gcagcgctcg ggggtgagcg
     2341 ttcgggtgac catcgcgaac cacgagacgc tggtcgcggc cgccctcaag cgggcggtgc
     2401 ggttggacga ggcgtcggcc gcgccacgca tcagcgacct ggggtccgtg gtcgcctcca
     2461 cggcgggaaa gatcgagctc gagacgctgg gcgacgagac gcgcgaggac cgcgtggtcg
     2521 agaagctggt cgcgcgggcg gtggtcaacg tcttcaaccg ccacttcgcg ctgagcgagc
     2581 tcggcccgct gttggatcgg ttcgaggagg agtcgctctc ggccgaggtg ggtgacctgg
     2641 ttccatcgag ccagcacgcg gccctggccg ggcgcgttcc ggagctggcc gccgccgttg
     2701 cccggctgga cgccggcgag tctccggcgg gtgtcgcctc ggccacggag ttcgtcctcg
     2761 aagggctgca cctcaaccga cggctcaaca aggagcgacg agcgggcggc gtgcggtacg
     2821 cccgctgacg aatcgggcgc aacggccctt cccgcgcagg tgatcgccgc ctgacgctgc
     2881 ctccgaacgg aggtgagtca gatgaccgtg ctgacgcaac cgcgggtcag cttcgagacc
     2941 gatgacgacc tgcgccgcct cgcgtcggcc agacgcgacc gggtgcggtt cgtgaacgtg
     3001 ccggcgtggc ggtacctggc gctcgacggg accgagcagc cgggcagcga ggcctatcag
     3061 caggcgatcg gcgtcctgta cgggacggcc taccggctgc acttcgccct gaaggcccgc
     3121 ggcgtcgatg cgccggtcgg tgggctcgaa gggctgtact ggctgacgcc ggagcagttg
     3181 gcggccgaag gcacgaccgg ctccgcgcag gacgtcgagt ggcggtggcg gctgatcatc
     3241 gccgtgcctt ccgaggccag cgaggatgag gttgacgatg ccgtgcggcg cggtgagccg
     3301 aacgacatgc tccgccgcct ctacctcgac cgctggacgg agggcccggc ggcccagatc
     3361 ctccacgtcg gcccgtacga cgccgagcgt ccgaccctgc gtcgcctgga gcaggcgatc
     3421 tccgctgccg gactccgggc ccgcggcccg caccacgaga tctacgtcaa cgacccgcgc
     3481 cgcgcgggcc cggagcggac gaagacggtc ctgcgccagg gggtcgaggc ggccggctga
     3541 cggaccgccc tcgacacgcg acagcggccg tcctgggacg gccgctgtcg ggtccgcgcc
     3601 gaggtgctca gatcggccgc ggcgcgtgat gcgtgtagct gcctccgctg acgtagcgga
     3661 ccacggcgtc gacgatggca ccgaggacga ttccgccgat cagcgccggc acgatgtaca
     3721 ggccgtcgaa gaccggaccc caggtgctcg cggcgccgaa tgcctcgctg ccgagccagc
     3781 cgccgacgag cacgccaacg gcggtcacga cccactcgta cgccagccgc ggctcaccga
     3841 tgaactgcgg gatcgcgccg atgaggatcg atgccgtgac gagcacgatc gcggcgatgc
     3901 ccgacatccc gacgttccat cccaacaggt cgagcatggt gtcacctccc tcgctcgaat
     3961 gtgacgtcga cgtcgagcat cggccggtgg tcggtgctcg cgcaagggcc atcggtcccg
     4021 actcgttcgt ggctgcaggc cggaccgcgc cctcagcggt cccggagccg ctcgacgatg
     4081 ggcgcgaagg cctccatggc gcttgccggg atgccatagg cggtgatgcc gagccgttcg
     4141 cgacgccgca gcaggcgctc gcggagctgc gaagccgtgc cgtagagcag gtagggcgac
     4201 ccgccgacgg ccgccggtcc ggcggacttc accagcgcgg acagtgacgc cggcagcggc
     4261 tggccgtcgc ccacgacccc ggcatcggcc acgaagacgt tcagctccag cagctcgaag
     4321 cggggacccg ccgccgcgcg caccaccgcc acccggtcgg cgagggctga ctcggtggtc
     4381 gtgcgaagca tcggtcgccc ggcggtgccc atctgcgggg cgaagccgac gatctgcgcc
     4441 tcgcgtgccg ccatgcgcag cagccgcggg ccgccacccc cgagcatgat cggcgggtgt
     4501 gggcgctgga ccgggagcgg gccgacccga gcgccctcca gccggtagaa gcggccctcg
     4561 tgcgtgaccg cttcgccgga cagcagccgc ttgatgaggc cgagcgcctc ctcgagccga
     4621 tcgatgcgca gccccggccg gtcgtacggc atcccgatgc gccggtagtc gctccgcatc
     4681 cagcccgcgc ccagtccgag gtccagccgt ccgccgctga gcacgtcgag tgtcgcagcc
     4741 tcacgcgcca ggatcagcgg atggcggaag tcgttggcga agacgtaggc gccgatgcgg
     4801 atgcggctcg tcagcgccgc tgcggtcgcc agcgcggcga tgggcgagag ctggcgtccg
     4861 aggtggtcgg tgacgtacag cgcgtcgtag ccgagctcct cggtccgccg gaccagggcc
     4921 gggaagtcgt gggtcagccg gcccccgccg gtgtggaccg cgaatcgaaa ggggtgcacg
     4981 cgcgaacggt actcgccgcc gactcaggcg acgatccggg ttccagcctc gcccgcgagc
     5041 gccgcctcca tccgatccgg gctggtgatg atggcctccc cgccgccgtg ctcgaggaac
     5101 tcgatgcacg cctcgatctt cggggccatg ctgcccgacg cgaaatgcgt gccctcggca
     5161 aggtaggact tcgcctccgc cagcgtgagg ctgtcgaccc agcgctgctc cggcttgccc
     5221 cagtccagcg ccacgcggtc gacggcggtg ctgatcatga agacgtcggc acgcaacgtg
     5281 gtcgccagca gcgccgatgc gaagtccttg tcgatgacgg ccgccacgcc ctccaggtcg
     5341 ccctggccgt tggtcacgac ggggatgcca ccaccgccga ccgtgatgac cacgaagtcc
     5401 tcgcgcagga gcgtcttcac ggcccgctcc tccacgatcc ggatgggccg cggtgagggg
     5461 acgacgcgac gccagccgcg gccggcgtcc tcgcgcacga cccagccgtc gcgctcctcg
     5521 cggatcctgg cctgcgcctc gtccatgaag gtgccgatcg gcttggaagg gttggcgaag
     5581 gcgggatcgg cgggatcgac ctcgacctgg gtcacgacgg tcaccgcctg ccgatcgatc
     5641 cggagctggc ggaagtcgcg gccgaggttc tgctggagct cgtagccgat ggcgccctgc
     5701 gtatcggcgt cgcagacgtc cagcggcacc tcgtgcagct cgtgcgcgga cagctcggag
     5761 cggcgcagga tgaagccgac ctgcggaccg ttgccgtggc cgatggcgac gtgccagccg
     5821 tccttgatga tccgcgcaat gtgcccgctc gtctcaccca gcgcccggta ctggtccggg
     5881 acgctcatgt gctgcgggtc cttgatcagg ctgttgccgc cgatcgcgac gaccagggtg
     5941 ctcttgctca tcggtcccta cctcatcgtc agcgccatga cggccttctg ggcgtggagc
     6001 cggttctcgg cctcgtcgta gacgacggac tgcggcgagt cgatgaccgc atcggtcacc
     6061 tcgacgttcc ggtcggccgg cagcgggtgc atgaagatgg cgtggtcgct cgccagggca
     6121 atgcgccgct cgtcggtgat ccagtcggtg tagcgctgtg cgatctcggc gccctccgtc
     6181 tcgtccttcg cggtcagcag cgcgccccag gacttggcgt agatgacgtc ggagtcccgg
     6241 aacccggcgt cgaagtcctc catcagctcc agcgagccac cggacgaccg ggcgttgagc
     6301 cgcgcctcct cgaccacgtc gggctccagc gcgaactccg gcggatggac caggcgcacg
     6361 ttcatcccga agcgggtggc cgccgtgacc aggtcctgcg ggacgctgat cggcttctgg
     6421 tagctggcgg cgtacgccca ggagagggtg agcgtgcggc ctcgcaggtc gcccagcttc
     6481 tcgcgcatgg tgagcaggtc ggccaggatc tggtgcgggt ggaagcggtc gcactgcatg
     6541 ttgagcacgg gcacgcgcga ggcctcggcc acgccgcgga tgtactcgtt gcccacgttc
     6601 cagtcgacgt ggcggatggc gatgccgtcg ttgtagcgac ccaggatctc gccgatctcc
     6661 ttggccgtgt cgccgtgcgc gatctgcgtc gtacggctct cgatgaactg ggcgtggccg
     6721 cccagctggg ccatgccggc ctcgaagctg gctcgcgttc gggtggagct gaagaagaag
     6781 agcatcgcga gcaccttgtc gcgcagcagc gggtgaccga tgcccagggc acgctcgcgc
     6841 ttcagctgcg cggcgacgtc caggatggtc tcgatctcct cgagcgtgag ctcggagagg
     6901 ctgatcaggt cgcgtccgcg gagtccggtc tgcatcggtc ctgtcctccc tactccatcg
     6961 cgccgaggac gagggccagg agcgccatgc gcagcgagac gccgttcgac gcctccaccc
     7021 agtagcgctg gtggcgcgtc tcgtcgacgt ccggcaggag ctcgtccatg cgcggcagcg
     7081 agtgcagcac gatcgacgac cgcttggcgc gcgaccgcat gagggctgcg tcgacctggt
     7141 actccgccgc cgtccgcccc acctcgccgc tgcgctcgac gcgcgactgg gtgtagtcgg
     7201 cctggaccac cggctcgagg tagatgatgt ccgcctcgcc gatgacctcg cggacgtccg
     7261 gcgcctcgcg gtagacggtg cccatcgcgt cgagctccgc cttgaactcg tccttgagcg
     7321 ccatgtccgg cggcgagacg acgatggcct ccgcgtcgaa ctgcgagagg gcgtggagga
     7381 tcgagtgcat cgtgcgcatc cgcatgtcgc cgatgagcag gtaggtcagc ccgtccagcg
     7441 tgccccgctc gagcaggatg gtgtacaggt cggtgagcac ctgggttggg tgctgccccc
     7501 agccgtcgcc gcagttgatg atcgggaccg atgcgtaccg ggccgcctcc gccggtgcgc
     7561 cctcggcgta gtgccgcatg gcgatcacat cgccgtagga ctccagcatc aggatggtgt
     7621 ccttcatcga ctcctggtag aagtcgccgg cgcgcgtcat cttggcgtcg ctgaagccca
     7681 ggacggcacc gccgagccga tgcatggcgg cctcggtcga cagccgggtc cgcgtcgagg
     7741 gctggtagaa ggccgtcagc agcgtcttgt gcttgagcag ctcgccgttc cgccggttca
     7801 cggcgtacgg gcgcagctcg ttggcggcgt cgaagacgcg gaagaagtcc tcccggctga
     7861 agtccttcag cccgaggatg tcgcgcccga ggaagcttct catcgtggat ctccagttcg
     7921 gcggatgcca gtgaagagga cgagcgacgt ctcggccggc gagaggtcgc cgtcccggtg
     7981 gagtcgaatc aggccggcca ggccggccga gccggtgggg tcgacgtcga tggacgtcgt
     8041 ggtgcgggcg agggcgttgg cattggcgat cgtcgcctcg tcgacgacga tcggaccgcc
     8101 gcgggtcagg agcatggcgc gcacgacggc cagccagtcg taggtctcgt cgtccaggat
     8161 cccgtgcgcg gcgctgccct ccgggtgctc ccacggccac atgtagccgg accgatgctt
     8221 cccggcccgt cgcagcacct
//
LOCUS       DASTYP010000218         6216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1732964, whole genome shotgun sequence.
ACCESSION   DASTYP010000218 DASTYP010000000
VERSION     DASTYP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732964"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..398
                     /locus_tag="VFV59_09550"
     CDS             189..398
                     /locus_tag="VFV59_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326112.1"
                     /translation="MSRDRIELQIRVIEVTFRSGRGDLEPLNMARVRARSRELLREVE
                     GAARQHPDLLARLERLRAELDDDEL"
     gene            complement(395..784)
                     /locus_tag="VFV59_09555"
     CDS             complement(395..784)
                     /locus_tag="VFV59_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5326113.1"
                     /translation="MTRITVVNDNPEFLALMRDILEDDRYVTVTVDGDQPDALERIRA
                     SRPDLLIIDLRLGTDGVHGWDIAQEVRRDPGLDALPVLVLSGDVATLREIEEDLAATQ
                     HTAGMAKPFGIDELERTIQRLLAEAAA"
     gene            1210..1380
                     /locus_tag="VFV59_09560"
     CDS             1210..1380
                     /locus_tag="VFV59_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326114.1"
                     /translation="MKAHLEMGESNLRYEDLKAAADENGRSVEETVDIMNRTIEIDRG
                     DHAQEYEGSAAG"
     gene            complement(1618..3195)
                     /locus_tag="VFV59_09565"
     CDS             complement(1618..3195)
                     /locus_tag="VFV59_09565"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5326115.1"
                     /translation="MLRRLCFDVPTLLSVLYAPLQPLVKRLRIVRRGDRRYQPNDVLL
                     PPGYGAEVVATGLTAPVHASFGPHGAMYVTESGHKSESPPRIFEVDPTSGSRRLVVEL
                     SGPHAQSTGAATGTAWHDGSLIVTATDAVLRVDPETGVTTELVDGLPGLGDHQTNHPV
                     IGPDGRVYWGQGCVTNMGVVGADNFGYEWLAKFRDRHDVPGGDIVLAGRNYEVPNVLG
                     NPLERLTTGAYVAFGTATEPNQRIPGSPKASGAVLSCLPDGSDLRVVAWGLRNPYGIA
                     VHPDGRLFVTEHGSDERGARWIVGDPDDLYEIREGAWYGWPDFASGIRLDDPHWGPGG
                     TGREPVLAEFPDPEPPKPVGSFEPHAAANGFDFSRDPAFGFEGQAFVALFGDLAPVTT
                     PRQVVPAGFKLVRVDPSTGAIHDFAVNRIQGPASKLFHGGFERPSHCVFGSDGALYVV
                     DFGEIKIAPEKGGIRMKQGTGSVWRISRVPDVPAGDVPPSATRVPFYPIQAGVVLALV
                     AGGVAAVVAVLRRLFRR"
     gene            complement(3195..4367)
                     /locus_tag="VFV59_09570"
     CDS             complement(3195..4367)
                     /locus_tag="VFV59_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5326116.1"
                     /translation="MRLLAGVSVGWLGISMLADGVPSLLVPHRVAATGGDASTLGLVT
                     LVAIGLAAVTQAFAGAWSDRIGRLPVVTAGALLAVAGLGLLLLPDALLPAAVLTLAGV
                     SVVQAGYQPLLPDRLAWSSRGRGAGAKGLFDVGGAFLGFALLAAILASGEATTATVVL
                     GAGLVGPLLLAFALLPTGRGPQQTAAPGPMHPGDRALLTRLVLARFLFLLGIYAVGRF
                     LLLFTAQRLGLDADAAAAQAGTALAILTLATAAAAVPSGWLADRIGRSAVMLAGGAIG
                     AVGVAALPLAGSLPAILVFGSLMAVGSAAFGAGSWAALADATAERSTGRLLGIANLGT
                     AGAAAAAGLFGPLIDAAGFGPAFALAGVLAAAGALVGWRAGASAPRPSLRPIEVFD"
     gene            complement(4368..4880)
                     /locus_tag="VFV59_09575"
     CDS             complement(4368..4880)
                     /locus_tag="VFV59_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014352401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU5326117.1"
                     /translation="MESRVKLLGHPVHPMLIVFPLGLLSTAVIFDVLYLVTGNDELAI
                     FAFWAIAAGIVGGLAAAIFGLIDWLAIPRDTRAKGVGLTHGIGNVVIVGLFAVSWLLR
                     LDNPVYGDNLLPFVLALIGAGLALFTAWLGGELVYRLRVAVDDGANLDASNSLTASGV
                     AEVTDARPRS"
     gene            complement(5285..5599)
                     /locus_tag="VFV59_09580"
     CDS             complement(5285..5599)
                     /locus_tag="VFV59_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326118.1"
                     /translation="MIQQRPATTDIARTGPWIALLLAAGLTVGFIAGQVTPDLLGPLG
                     GSSAEVVTTDTLTGADDYGIRHLTVAPPLTQADDYGTRHAGTAPLTPSDDYGIRHGAS
                     QP"
     gene            5746..>6216
                     /locus_tag="VFV59_09585"
     CDS             5746..>6216
                     /locus_tag="VFV59_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEU5326119.1"
                     /translation="METAAVTAGVAGTIEAIYRQEADRLWRALFAFAADAEIASDSVA
                     EAFAQAIRRGPAIRDARAWVWKAGFRIASGELKRRSRTTDAIPEGWYTEPEADAELLA
                     ALARLPDGQRAAVILHYYTDASVREIAQRTGMSQLSVRANLSRGRKRLKLLLGDR"
BASE COUNT          990 a         2071 c         2308 g          847 t
ORIGIN      
        1 ggcaggtcgc tgcgggtccc cacgcgcgga tcatgcacgc tcgagtggtc ggggcgccag
       61 aggaagggag ctgacttgct gtggcggcct atgcctccga cgagtgggag gcggccgggg
      121 ccctcctacc gcagtgggct cgtagcggcc acaggctggg cacgctgctt gcgcctgagc
      181 gccgtccaat gtcacgcgac cgcatcgagc tgcagatccg ggtcatcgag gtgaccttcc
      241 gctcaggtcg cggggatctc gagccactga atatggcgcg ggtgcgggct cggtcgcgcg
      301 aactgctgcg tgaggtcgag ggcgcggcgc gccagcatcc ggatctgctc gccaggctgg
      361 agcgcctgcg cgcggagttg gacgacgacg agctctaggc ggcggcctcg gcgaggagcc
      421 gctggatggt gcgctccagc tcatcgatgc cgaacggctt ggccatgccg gcggtgtgct
      481 gcgtggccgc caggtcctcc tcgatctcac ggagggtcgc gacgtccccg ctgaggacca
      541 gcaccggcag agcgtccagc ccggggtcgc gacgcacctc ctgggcgatg tcccaaccat
      601 ggacgccatc ggtcccaagg cggaggtcga tgatcagcag gtcgggcctc gaggcccgga
      661 tgcgctccag ggcgtccggc tggtcgccgt cgacggttac cgtcacgtag cggtcgtcct
      721 cgaggatgtc gcgcatcagg gcgaggaact cggggttgtc gttgacgacg gtgatacgtg
      781 tcacgggagc cggaccatcg tgttctacgc gtggacctcg ggaaggggtg ccgtcgcggg
      841 gacggcggcc gcttcgcgtt ccgcctgccg acaccgtcgg caatcgtcgt ggggttggct
      901 atggaggccg cgcatgtgtt ctgctgcgat cgtcaagagg cgcccgggct caggtaagac
      961 gttcttgttg tccataaagg gactccttcg catcatggtg cgaaggggcc atctcattgg
     1021 tggccggacg acgcagaagc cagtgtacgg cttcgtgtcc accacttctg ccgcgtccat
     1081 gcgctccgag cgatgggcac ccgcgaacgt acgcccccgg atgaggcggt atggcctgtc
     1141 ggatgcaacg tcgtccgtca acgtgccccc ggaagaggag ttgaatcgat gtgtctcacg
     1201 tgtggctgca tgaaggccca tctcgagatg ggcgaatcga acctgcgcta cgaggacctc
     1261 aaggccgccg ctgacgaaaa cggccggtcc gtcgaggaga cggtcgacat catgaaccga
     1321 acgatcgaga tcgaccgagg cgaccacgcg caggagtacg agggctccgc ggcgggctga
     1381 gggccgcacg attggcgggc aggcttggcc cgcaattggg accgcgccct tgctggggcg
     1441 ggaggcatgc gttctgggct gggttgggct ggagcggaac taacggcgaa gaaggcggct
     1501 ggcgatcggt ttgccgattc gtgaggttgc ggcgaggagc aaccggcggc gaagcatcgg
     1561 ctgcagatcg gcgcctgcgt gtccgttagt tccgctgggc ggcaactcca cgaagcgcta
     1621 ccggcggaag agccggcgga gcaccgccac caccgctgcg accccgccgg cgaccagcgc
     1681 gagcacgacg ccggcctgga tcgggtagaa cggcacgcgg gtggcggacg gcggcacgtc
     1741 accggcaggc acgtcgggca cgcgcgagat gcgccacacc gatccggtcc cctgcttcat
     1801 ccggatgccc cccttctcgg gcgcgatctt gatctcgccg aagtcgacga cgtacagcgc
     1861 gccatcggac ccgaagacgc agtggctggg ccgctcgaag ccgccgtgga acagcttcga
     1921 agcggggccc tggatgcggt tgaccgcgaa gtcgtggatg gcgccggtcg acggatccac
     1981 gcgcacgagc ttgaagccgg ccggcacgac ctggcgcggc gtggtgaccg gcgctaggtc
     2041 gccgaaaagc gcgacgaacg cctggccctc gaagccgaag gccggatcgc ggctgaaatc
     2101 gaagccgttg gccgccgcgt ggggctcgaa cgagccgacc ggcttgggcg gctcgggatc
     2161 cggaaactcg gccagaaccg gctcccggcc ggtcccgccc ggaccccagt gcggatcgtc
     2221 caaccggatg ccggaggcga aatccggcca gccgtaccag gcgccctcgc gaatctcgta
     2281 gaggtcgtcg gggtcgccga cgatccaccg ggcgccgcgc tcatcggacc cgtgctccgt
     2341 cacgaacagg cggccgtcgg ggtggacggc gatcccgtac gggttgcgca ggccccaggc
     2401 gacgacgcgc aggtccgatc catcgggcag gcagctgagc accgcgccgc tggccttcgg
     2461 gctgcccggg atgcgctggt tgggctccgt ggcggtaccg aacgccacgt acgcgccagt
     2521 cgtcaggcgc tcgagcgggt tgccgaggac gttcggcacc tcgtagttgc ggccggccag
     2581 cacgatgtcg ccgcccggaa cgtcgtgccg atcccggaac ttggccagcc actcgtagcc
     2641 gaagttgtcg gcgccgacga cgcccatgtt ggtgacgcag ccctggcccc agtagacgcg
     2701 gccgtcgggg ccgatgaccg ggtggttcgt ctggtggtcc ccgagccccg ggaggccatc
     2761 gacgagctcg gtggtgacgc ccgtctcggg gtcgacgcgc aggaccgcat cggtggcggt
     2821 gacgatcagc gaaccgtcgt gccacgcggt gccggttgcg gcccccgtcg actgcgcgtg
     2881 cgggccactg agctcgacca ccaggcgccg gctgccactc gtcggatcca cctcgaagat
     2941 gcgcggcggt gactccgact tgtgccccga ttcggtcacg tacatggcgc catggggacc
     3001 gaaggaggca tgcacgggag ccgtcaagcc ggtggcgacc acctcggcgc cgtagcccgg
     3061 cggcagcagg acgtcgttag gctggtatcg ccgatccccc cgccgcacga tgcggagccg
     3121 cttgaccagc ggctggagcg gcgcgtacag caccgagagc aatgtcggaa cgtcgaagca
     3181 gagacggcgg agcatcagtc gaagacctcg atgggacgga gcgacggtcg cggagccgat
     3241 gcgccggcgc gccacccgac gagcgcgcca gcggccgcca gcactcccgc gagggcgaag
     3301 gccgggccga acccggcagc atcgatcagc ggcccgaaca ggcccgcagc ggcggccgcg
     3361 ccggcggtgc ccaggttggc gatgccgagc agccggccgg tggagcgttc ggcggtcgca
     3421 tcggccaggg cggcccagct cccggcgccg aaggctgccg acccgacggc catgagcgag
     3481 ccgaagacca ggatcgccgg cagcgagccg gccagcggca gcgcggcgac gccgaccgcg
     3541 ccgatcgcac ccccggcgag catcaccgcc gatcgcccga tgcggtccgc cagccatccc
     3601 gacgggacgg cggcggcggc ggtcgcgagg gtgaggatcg ccagggcggt gccggcctgc
     3661 gccgcggccg catcggcgtc caggcccagg cgctgggcgg tgaacagcag gaggaatcgg
     3721 ccgaccgcgt agatgccgag caggaagagg aagcgcgcga ggaccaggcg cgtgagaagg
     3781 gcgcggtcgc cgggatgcat cggtcctgga gcggcggttt gctgtggccc tcggcccgtc
     3841 ggcagcagcg cgaaggccag gagcaggggc ccgaccaggc ctgcgccgag cacgacggtg
     3901 gcggtggtcg cttcgccgct ggcgaggatg gccgcgagca gggcgaagcc gaggaaggcg
     3961 ccaccgacgt cgaacaggcc cttggccccc gcccctcgcc cgcgggagga ccacgcgagc
     4021 cggtccggca ggaggggctg gtagccggcc tgcaccacgc tcaccccggc gagcgtcagg
     4081 acggcggcgg gcaggagggc atcggggagg aggagcaggc ccaggcccgc gacggcgagg
     4141 agcgcgccgg ccgtcacgac cggcaggcgt ccgatgcgat cgctccaggc gcccgcgaac
     4201 gcctgggtga ccgccgccag accgatcgcg accagcgtca cgagccccag cgtcgaggcg
     4261 tcaccgcccg tcgcggcgac ccggtgcgga accagcagcg acggaacccc atcggccagc
     4321 atgctgatgc ccagccatcc gacgctcacc cccgccagga ggcgcattca ggatcgcggg
     4381 cgcgcgtccg tgacctctgc cacgccgctg gccgtcagtg agttggaggc atccaggttg
     4441 gcgccatcgt cgaccgccac gcgcagccga tacaccagct cgccgcccag ccacgccgtg
     4501 aacagcgcca gtccggcacc gatcagcgcg aggacgaagg gcaacaggtt gtctccgtag
     4561 accgggttat caaggcgcag cagccagctc accgcgaaca gtcccacgat cacgacgttg
     4621 ccgatgccat gggtcaggcc cacgcccttg gcccgcgtgt cgcgcggaat ggcgagccag
     4681 tcgatcaagc cgaagatcgc cgcggccaga ccgccgacga tgcccgccgc gatggcccag
     4741 aacgcgaaga tggccagctc gtcgttgccg gtgacgaggt acagcacgtc gaagatgacg
     4801 gcggtgctga gcaggccgag cgggaagacg atgagcatcg ggtggaccgg atgcccgagc
     4861 agcttgacgc gagactccat caccctcacc tctgtgattg gcgtgtcctg tatgaacagc
     4921 cgccgtttgc gttacagcag aagccgtgcc ggtcgggccg attctccggt gcctcggtcg
     4981 attggcattg ccgtggggcg ggtgaatcgg gcaggaagtg gccgatcgtg ccaccgggtg
     5041 gcaacacgga gcggcgggtt ggtctgggcc ggaactaacg gcgaagaatc gcgccacaag
     5101 ggccactggt tggccaagag ttgcgaggtg gagcaacggg cgatgatgaa aggagcgggt
     5161 gccggccgct gcgtgtccgt tagttccgct ggggagcaac cgtgaggacg ggaaggggtc
     5221 caggccgggc tggagggatg caggtcccca gcccggccgg tgtcatcgac gccgaagcga
     5281 agccctaagg ctgcgacgcg ccgtggcgga tgccgtagtc gtccgacggc gtcagtgggg
     5341 cggtgccggc atggcgggtg ccgtagtcgt cggcctgcgt cagcggcggc gcgacggtca
     5401 ggtggcggat gccgtaatca tcggcgccgg tgagcgtatc ggtcgtcacg acttctgccg
     5461 agctgccgcc aagcgggccc aggaggtcgg gcgtcacctg tcccgcgatg aagccgacgg
     5521 tcaggccggc ggccagcagc agcgcaatcc aggggccggt gcgggcgata tcggtggtgg
     5581 ctggtcgttg ctggatcatc ggtctcggtc tcctgtcggt ctgcgttcga gtccctccgc
     5641 ctacacaggg acccgacggc ggccaaaggt gacatcgaat ttcgacctcg accaacgggc
     5701 cggcgtgtgt cacctttcag cccgattcgc tccctgtagg agcagatgga gactgcggca
     5761 gtcaccgctg gtgtggctgg cacgatcgag gcgatctacc gccaggaagc cgatcggctc
     5821 tggcgtgcgc tgttcgcctt cgccgccgat gccgagatcg ccagcgattc cgtcgcggag
     5881 gccttcgcgc aggccatccg ccgcggaccc gccattcgcg atgctcgcgc ctgggtctgg
     5941 aaggccggct tccgcatcgc ctcgggcgag ctgaagcgac ggtcgcgcac cacggacgcc
     6001 attccggagg gctggtacac cgagccggag gccgatgccg agctgctggc cgcgctcgcc
     6061 cggctgccgg acggccagcg cgccgccgtg atcctccact actacaccga tgcgtccgtg
     6121 cgcgagatcg cgcagcggac cggcatgtcg cagctctcgg tccgagccaa cctgagccgc
     6181 ggccgcaagc gcctgaagct gctgttgggt gaccgt
//
LOCUS       DASTYP010000219         5954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1660962, whole genome shotgun sequence.
ACCESSION   DASTYP010000219 DASTYP010000000
VERSION     DASTYP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660962"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFV59_09590"
     CDS             complement(<1..596)
                     /locus_tag="VFV59_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU5326120.1"
                     /translation="MSDEVLRRAAELASAFLRELPERHVGARADRSALMAAMSGPLPE
                     DGEDPLTVVEALASAADPGLIAMPGPRYFGFVIGGSLPSTVAAEWLTAAWDQNAGIYT
                     TSPAASVVEEVAAGWLLELLDLPATASVGFVTGGQMANFTCLAAARHAVLARAGWDVE
                     ADGLADAPRVTVIVGEEAHATALTALGYLGLGRERAIR"
     gene            681..1118
                     /locus_tag="VFV59_09595"
     CDS             681..1118
                     /locus_tag="VFV59_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326121.1"
                     /translation="MTARAQAVGEDRQALVRFVFGLTLGVGAMAALAGGLTGQPSVVV
                     AVPTVVLAVALWRWRGDGLPHPAAGWAGALVWLVLATLTRDDTVLVPLAMAAGCATIA
                     IGPERVLSWLGAQWMRDEPAAEPPASVEPPADPGWLEEDGRRL"
     gene            complement(1140..1925)
                     /locus_tag="VFV59_09600"
     CDS             complement(1140..1925)
                     /locus_tag="VFV59_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5326122.1"
                     /translation="MDLGLRGRTAVVGGATSGLGRASAEVLAAEGCDLFLWSRSAERL
                     EATAGALRAAHGVRVAWTAADAADPTAAATVAEAALEFAPIDILVSNAGGPPAVEPTQ
                     TDAEGWQRAFQLLASTPVDLATRLLPGMRERGWGRVVAILSYGVRQPINELVYSNAGR
                     GALAAWLKTTSRVVAADGVTVNGVLPGRLNTPRIRELDAGRAEREGTTEEAVRAAYLA
                     AIPAGRYGRPEELGALVGFLASDRASFITGQFVAVDGGLVAGY"
     gene            complement(1929..3008)
                     /locus_tag="VFV59_09605"
     CDS             complement(1929..3008)
                     /locus_tag="VFV59_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU5326123.1"
                     /translation="MFRPLQAIFAAPFIRRLGFHAKRIGGQMDRHFFTSLLTAVIGFV
                     VISAIAVTLLEDDKRSFAGFGSAFYWAVTTVIGSGDPSFVNSPGGFVIGWLLAFFGVA
                     IVAAMTGAVVGFVIDFLLKEGQGMGAAGYRDHIVVCGWNTTARDLIEELKGDEFQTKV
                     VVLHDSERNPAGDGVYYVRGDITSTEDLDRCGIREAASAIVFPADRTNEADMRSILAV
                     MAIESIAPEVRTVVEANNPSHVDHFKRAEADEILVTSRLASRLLARSALYPGLSELVT
                     DIVSGGEGSELYRVALPDDYVGLSIDELSAKLRGEHRATLLAVTRGGRSMTNPPADFR
                     LQAGDDAVVVAESLGELRPLELQHN"
     gene            3198..5243
                     /locus_tag="VFV59_09610"
     CDS             3198..5243
                     /locus_tag="VFV59_09610"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HEU5326124.1"
                     /translation="MPRSLVLAGSVATLLAALLVTALPALSPDVVRAADIDVRVDACS
                     LLEDSTVAELTGVATTYATDSRTGSSDSHCFWGSTRPGEPGYVELSIVRQHSLSGYSF
                     GDGCDVTTVDDLGEEAAFVDCPPDPQKKISLLAYERGAIVILLVNEPAAGLTADDLAT
                     VVRSVLQQLGSVAASEPPAASDDPVDACSLLTAAEVEAVTGLAAEPGVMKETETVGQV
                     GCGFGTVPYEVQVAVYPSLGGAAVDGLGDSGHPVEGVGDGAEWWWCEECLAVDASSST
                     NRLVVASGTWYVDIGFAPTTDQAADDSELVQATHLEWAVELVRLILPRLEGPVDRPEP
                     SADADPDSAPAGDSGEVGSTDGGSSRPFASSVPTAADVSTDPAVLLQSALLAALLVFL
                     MPFPSQLFNSTLETHEDEVRRWLRLDRVGGAASRIGAFWASWPGVIAFTLLATLLYAF
                     LDPAFGLDVGSLATFLGMLLGIILVTAAFSIPVALAHRRHGDRPSVKVVPISLLVGVA
                     CVLLSRLTGFQPGYLYGLLIGLAFARELSAAEEGRATAIGAALMLATAVACWLALGVV
                     PTGGAFALVVVRTALAALMVAGLEGVVFGLLPMRFLPGEPLYAWNRIAWAALLGMGAF
                     AFFHILINPASGYLSDTSRTPLFTVAALLLGFSLVSVGFWAWFRFRPDPTSPVESTH"
     gene            5288..5740
                     /locus_tag="VFV59_09615"
     CDS             5288..5740
                     /locus_tag="VFV59_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326125.1"
                     /translation="MIAFVSRSATVVLAVLVLAACGAGARATTSASGPAASEVPSPSA
                     NAPSQAATSTPSQAEPSEDGAPTACLDADVLAALEAYKDVEVPAEPSMDEVADAFEAL
                     ELEGRAAEYRDSLVEALRDDSETLDSMKLQSIMFSLLPLQSEVNLVEC"
BASE COUNT          797 a         2127 c         2130 g          900 t
ORIGIN      
        1 atgcggatgg cgcgctcgcg acccaggccc aggtagccga gggcggtcag cgcggtcgca
       61 tgggcctcct cgccgacgat caccgtcacg cgcggtgcgt cggcgagccc gtccgcctcg
      121 acgtcccacc ccgcgcgcgc cagcacggcg tgtcgggccg ccgcgaggca ggtgaagttc
      181 gccatctggc cgccggtcac gaagcccacg ctcgcggtgg ccggcaggtc gagcagctcg
      241 agcagccacc ccgccgcgac ctcctcgacg accgatgcgg ccggacttgt ggtgtagatc
      301 cccgcgttct ggtcccaggc ggcggtcagc cattccgcgg ccaccgtcga cggcagcgac
      361 ccaccgatca cgaagccgaa gtaccgcggt ccgggcatcg cgatcagccc gggatccgcc
      421 gccgatgcca gggcctcgac caccgtcagc gggtcctcgc cgtcctccgg cagcggcccg
      481 ctcatggcgg ccatcagggc ggaccgatcg gcgcgggcgc cgacgtggcg ctcgggcagc
      541 tcacgcagga aggcggaggc cagctcggcc gcccggcgca ggacctcgtc gctcatggcc
      601 accgatccta caccccggtc cgagcggctc tggtcgccgc gtccgcgccg gcgtagggtt
      661 gtcgagagcg aggtgacgcc atgacggctc gagcccaggc ggtcggggag gatcgccagg
      721 cgctggtccg gttcgtcttc gggctcacgc tcggtgtcgg cgccatggcg gcactggccg
      781 gcgggctgac cgggcagccg agcgtggtgg tggcggttcc gacggtcgtc ctggccgtcg
      841 cactgtggcg gtggcgcggc gacggcctgc cgcatcctgc cgccggttgg gccggagcac
      901 tggtctggct cgtgctcgcg acgttgacgc gtgacgacac ggtgctcgtc ccgctcgcca
      961 tggctgccgg gtgcgccacc atcgcgatcg gtccggagcg ggtcctctcc tggctgggcg
     1021 cgcagtggat gcgcgacgag ccagccgccg agccgccggc aagcgtggag ccacccgccg
     1081 atcccggctg gctcgaggag gacggccgtc gcctctgatc tcgtcgagtc ggcggaacgt
     1141 cagtagccgg cgacgaggcc cccgtcgacc gccacgaact ggccggtgat gaagctcgcg
     1201 cggtccgagg cgaggaagcc gaccagcgcg cccagctcct cgggccgccc gtagcggccc
     1261 gccgggatgg cggccaggta cgcggcgcgg accgcttcct cggtcgtgcc ctctcgctcg
     1321 gcgcggccgg cgtcgagctc ccggatgcgg ggagtgttca ggcgtcccgg caggacgccg
     1381 ttgaccgtca cgccatcggc ggcgacgacg cgcgacgtgg tcttgagcca ggcggccagg
     1441 gctccacggc cggcgttgga gtagaccagc tcgttgatcg gttggcgaac gccgtaggac
     1501 aggatcgcca cgacccggcc ccagccgcgc tcccgcatgc cgggcagaag ccgggtcgcg
     1561 aggtccacgg gcgtgctcgc cagcagctgg aaggcgcgct gccagccctc ggcgtcggtc
     1621 tgcgttggtt cgaccgccgg cggaccaccg gcattcgaga ccaggatgtc gatcggcgcg
     1681 aactcgagcg ccgcctcggc caccgttgcg gcggcggtcg ggtcggccgc atcggcggcc
     1741 gtccaggcca cgcgcacgcc gtgggccgcg cgcagcgcac cggccgtggc ttccaggcgc
     1801 tcagccgatc gcgaccagag gaagaggtca cagccctcgg ccgccagcac ctcggccgag
     1861 gcccggccga ggcccgacgt cgcgccaccg accaccgcgg tgcggccgcg cagtccgaga
     1921 tccatcggtc agttgtgctg gagctcgagg ggccggagct cgcccagcga ctcggccacg
     1981 accaccgcgt catcgccggc ctggagccgg aagtcggccg gtgggttcgt catggaccga
     2041 ccgccgcgcg tcacggccag gagcgtcgcc cggtgctcac cacgcagctt cgcggagagc
     2101 tcgtcgatcg agaggccgac gtagtcgtcc ggcagcgcga cgcggtacag ctccgacccc
     2161 tcaccacccg acacgatgtc cgtcaccagc tcggagaggc cgggatacag cgcggatcgg
     2221 gcgaggaggc gcgaggccag gcgagaggtg acgaggatct catccgcctc ggcgcgcttg
     2281 aagtggtcga cgtgggacgg gttgttggcc tcgaccaccg tgcggacctc gggcgcgatc
     2341 gactcgatgg ccatcacggc caggatggag cgcatgtccg cctcgttggt gcggtcggcc
     2401 gggaagacga tggccgacgc ggcctcgcgg atcccgcagc gatcgaggtc ctcggtcgac
     2461 gtgatgtcgc cgcgcacgta gtacacgccg tcccccgcgg ggttgcgctc gctgtcgtgg
     2521 aggacgacga ccttggtctg gaactcgtcg cccttcaact cctcgatcag gtcgcgggcc
     2581 gtggtgttcc agccgcagac gacgatgtgg tcccgatagc ccgcggcacc catgccctgg
     2641 ccctccttga gcagaaagtc gatgacgaag ccgacgaccg cgccggtcat ggcggccacg
     2701 atggcgacgc cgaagaacgc gagcagccag ccgatgacga agccgcccgg cgaattcacg
     2761 aagctggggt caccggagcc gatgacggtc gtgaccgccc agtagaacgc gctgccgaaa
     2821 ccggcgaagg agcgcttgtc gtcctcgagc agcgtgaccg cgatggccga gatcaccacg
     2881 aagccgatga cggccgtcag cagcgacgtg aagaagtggc ggtccatctg gccgccgatg
     2941 cgcttcgcgt ggaagcccag ccgccggatg aacggggcgg cgaagatggc ctgcagcggc
     3001 cggaacaatc gtggtcctcc gtggcagctc gatgccaccg cgatgcgcga cggcgcgcgc
     3061 attctgccat cggctcggcg tgccgggcat cggtcattgc gcatagatcg ccgcttcgaa
     3121 attggttctt gccaacccga cggcgcgtcg ccatgattcg cgcgattcgg tagtgcccgc
     3181 cgtctagggg gaactcggtg ccacggtcgc tcgtcctcgc cggctcggtg gccaccctcc
     3241 tcgccgcgtt gctcgtcacc gcgctccccg ccctatcgcc ggatgtggtc cgcgcagcgg
     3301 acatcgacgt gcgcgtggac gcgtgctcac tgctcgagga cagcacggtc gccgagctca
     3361 ccggtgtcgc gacgacctat gcgaccgaca gcaggaccgg ctcgtccgat tcgcactgct
     3421 tctggggctc gacgcggccc ggtgagccgg gatacgtgga gctgagcatc gttcggcagc
     3481 acagcctgtc cggctactcc ttcggcgacg gctgcgacgt cacgacggtg gacgacctgg
     3541 gcgaggaagc ggccttcgtg gactgccctc cggaccccca gaagaagatc tccctgctcg
     3601 cgtacgagcg cggcgcgatc gtcatccttc tcgtcaacga gccggcggct gggctcaccg
     3661 cggacgacct ggcaaccgtg gtgcgatcgg ttctgcaaca gctcggaagt gtggcggcca
     3721 gcgagccgcc ggcggcgtcg gacgaccctg tcgacgcgtg ctcgctcctg acggcggcgg
     3781 aggtcgaagc cgtcaccggg ctcgccgcgg agcccggtgt gatgaaggag accgagaccg
     3841 tcggacaggt cggctgtggc ttcgggaccg ttccgtacga ggtccaggtc gcggtctacc
     3901 cctcgctcgg cggagccgcg gtcgacgggc tcggagactc cgggcatccg gtggagggcg
     3961 tcggggacgg cgccgagtgg tggtggtgcg aggagtgcct ggcggtcgac gcgagcagca
     4021 gcacgaaccg gctcgtcgtc gccagcggga cgtggtacgt ggacatcggc ttcgccccga
     4081 cgacggatca ggcggcggac gattcggagc tcgtccaggc gacgcacctc gaatgggccg
     4141 tcgagctggt ccggttgatc ctcccgcggc tggagggacc ggtcgaccgc cccgagccga
     4201 gtgccgacgc cgaccccgac agtgctcctg ccggggattc gggcgaggtc ggctcaaccg
     4261 acggcggttc cagccggccc ttcgcttcct ccgtgccgac cgctgcggat gtctcgaccg
     4321 atcccgccgt cctgctccag agcgccctcc tcgccgccct cctcgtcttc ctgatgccat
     4381 tccccagcca gctgttcaac tcgaccctgg agacgcacga ggatgaggtg cggcgctggt
     4441 tgcggctcga ccgggtcggc ggagccgcga gccgcatcgg cgcgttctgg gcgtcgtggc
     4501 ccggcgtgat cgcgttcacg ctgctggcga cgctgctcta tgcgttcctc gacccagcct
     4561 tcgggctcga cgtcgggtcg ctcgccacat tcctcggcat gctgctcggc atcatcctcg
     4621 tcacagcggc gttctccatc ccggtcgcgc tggcgcatcg gcgccacggc gaccggccga
     4681 gcgtgaaggt ggtgccgatc tcgctgctcg ttggcgtcgc ctgcgtgctc ctgtcgcggc
     4741 tcaccgggtt ccagccgggg tacctctacg gtctcctcat cggcctggcc ttcgcgcgcg
     4801 agctgtcggc ggccgaggag ggtcgcgcca ccgccatcgg tgcggcgctg atgctcgcga
     4861 ccgcggtcgc ctgctggctg gcgctcggcg tggtgccaac cggcggcgcc ttcgcgctcg
     4921 tcgtcgtgcg cactgcgctg gcggcgttga tggtcgcggg gctcgagggc gtcgtcttcg
     4981 ggctcctgcc gatgcgcttc ctgccaggcg agccgctgta cgcctggaat cggatcgcct
     5041 gggcggcgct gctcggcatg ggcgccttcg ccttcttcca catcctcatc aatcccgcgt
     5101 ccggctacct gtccgatacg agccggactc cactcttcac ggtcgctgcg ctgctgctcg
     5161 gcttcagcct cgtctccgtc ggcttctggg cctggttccg gttccgaccg gatcccacgt
     5221 cgcccgtgga gtcgacgcat tgaccggcga ccatcgcggt cgccgcacga caggaggacc
     5281 tcgacccttg atcgccttcg tcagtcgttc cgccaccgtg gtactggccg tcctcgtcct
     5341 cgctgcctgt ggggcaggag ctcgagcgac cacaagcgcg tcgggtccgg ccgcgagcga
     5401 ggtcccgtcg ccgtccgcca acgccccatc gcaggcggcg acatcgacgc cgtcgcaggc
     5461 ggagccatcg gaggacgggg cgccaacggc ctgcctggac gcggacgtcc tcgcggcgct
     5521 ggaggcgtac aaggatgtcg aggtccctgc cgagccgtcg atggacgagg tcgctgatgc
     5581 cttcgaggcg ctggaactcg aaggccgtgc cgccgagtac cgcgacagcc tggtcgaggc
     5641 gcttcgcgac gactccgaga cgctggactc gatgaagctg cagtcgatca tgttctcgct
     5701 gctgcccctg cagtccgagg tcaacctcgt cgagtgctga gtctcccgtg ccctggccgc
     5761 agcatcggtc gattggcgga aggccgcgcg gcagtggttg gccgatgcat cgcccgcgcc
     5821 tccggcgacg gcccggcgac cggcaaccgc ggcagcgcgg cctgatccac ccgggacgcg
     5881 ccgtcgtatc gagacgtggg gccgattggc cctcgccgct gctctcgcgt gtgcggagca
     5941 tcggtgcaga acca
//
LOCUS       DASTYP010000220        13642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_277435, whole genome shotgun sequence.
ACCESSION   DASTYP010000220 DASTYP010000000
VERSION     DASTYP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13642
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277435"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1418
                     /locus_tag="VFV59_09620"
     CDS             84..1418
                     /locus_tag="VFV59_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5326126.1"
                     /translation="MAIETERGTMTGDEIVALTKRHTLFEWSAQSKVDPIPVAGAKGV
                     YFWTPEGKRFLDFNSQLMCTNVGHGHPKVVEAIKQQAETLAYANPFMATEPRARLGQK
                     LAELTPGDIDTFFFTNGGAEANENAIKLARFVTGKHKILARYRSYHGGTAGAISLTGD
                     PRRWAAEPGIPGVVHVLDPYHGIQRGWDSADEALANLEETIQLEGPNTIAAFILETVT
                     GTNGILVPPDGYLQGVREICDRYGILMIADEVMAGFGRTGKWFAIDHWDVVPDLITMA
                     KGLTSAYVQLGALGMRRKYAEHFENNVFYGGLTYNSHPLGCATALATIAVYEEENLIE
                     RAAQMGEVMRGHMEELAAKHPSVGAVRNIGLFGIVELVRNRETMEPMAPFNGTSDEMA
                     ALGKRFRSDGLYTLVRWNYFFTNPPLTISEEEMAEGFAIIDRALEITDAAVA"
     gene            1402..2274
                     /locus_tag="VFV59_09625"
     CDS             1402..2274
                     /locus_tag="VFV59_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5326127.1"
                     /translation="MPPSPERGWLRRALVFGVFLALLLLVWEGLKFIGGDPWRTETWT
                     WDPPLKIWFASDLLLPHVWDIFGALSNPVQRGTDQSLAGFLVGAAFYTWREAAIGFVV
                     GAAIGIGLAALFVHSRLLERALVPYVIASQTIPIVALAPMIVFAFGQEVISVVIIATY
                     LTFFPVTIAMIRGLRSPDPRAIELMRSYAASRWSVFWKLRMPASLPYLFTALKISATA
                     SIVGAIVGEGPGGIPDGLGRAIINFNSQYVSGPEKLWAAILACAVVGIAFFTIVRVAE
                     VLTLRGRGIRPEVA"
     gene            2297..3145
                     /locus_tag="VFV59_09630"
     CDS             2297..3145
                     /locus_tag="VFV59_09630"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5326128.1"
                     /translation="MTDSAPRASVVRIRGVDKQFTAGAGAVTALSGIDLGIEAGEFVS
                     LIGPSGCGKSTLLRLIGDLTPPTSGSVEVNGKPAHQARLGREYGMVFQAPVLFEWRTV
                     EDNVKLPLELLGMDRSERDARAREMLSLVELGDFARHHPYQLSGGMQQRVAIARALAL
                     QPDILLMDEPFGALDEMTRERMNSEVLRIWRQTGTTVVFVTHSIPEAVFLSTRVVVMS
                     ARPGRITRVVDVPLPQPRNEETREDPRYFRVVTEVREALRAGGGLGEEAPLASEERVA
                     AEGGIG"
     gene            3142..3996
                     /locus_tag="VFV59_09635"
     CDS             3142..3996
                     /locus_tag="VFV59_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5326129.1"
                     /translation="MTAAAPSVGVPVPPRPGRMLRFVRTLRYYVPALVVFVSVIAVWE
                     VATRALDVRAFILPPPSAIGTALVENWGAGRWPLFDAARTTLLEAVGGLLIGTLAGVV
                     VAFAVGRWMNARDAVLPFAVAANAIPIIAIAPIFNAWFGITNPVSKMMVAALLVFFPV
                     MINVTRGLTQVEPSALELMRSYAASEWTILRKVRIPNALPYFLTALKVATTLSLIGAI
                     VGEYFGGSTSVIGRVVVQSASSLRFDVTWAAILLGAIAGIVFYLAIVALERVLIPWHA
                     SVRGEEHG"
     gene            4108..5253
                     /locus_tag="VFV59_09640"
     CDS             4108..5253
                     /locus_tag="VFV59_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5326130.1"
                     /translation="MKRSRIWLSLAMSASLVLAACGTQSGGSAGASADESSAASDGGG
                     GGEMTSVRLQLQWAPQAQFAGYFAAQELGYYEDEGLDVQILDGGPDVIPQQVGSAADG
                     PEFTISWVPKVLEAREAGSDLVDIAQVFQRSGTLSVAWADSGISGPADYEGKKVGVWD
                     FGNEWEVIAAAKEAGLEQGTDFERVIQNFDMVAFLNREIDVAEAMIYNEYAQVLEAEN
                     PDTGELYQPEDLTVIDYNEVGTAMLQDAIFARASWLAEDGNEDVATRFLRASFRGWIH
                     CRENPDDCVEFTVNAGSTLGMGHQRWMMNEINPLIWPSPDGIGMLDTDLWQQTVEISM
                     DSGIITAEPGEDAYRTDLAQAALDGIEEDATGESFTKGSVEVTPGGE"
     gene            complement(5311..6549)
                     /locus_tag="VFV59_09645"
     CDS             complement(5311..6549)
                     /locus_tag="VFV59_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5326131.1"
                     /translation="MDHLAEILLQLFVLLLAAKIGDEIFKRLGQPTVVGEILAGVVVG
                     PALLGWYEINAETQLFAEIGVVLLLFQVGVETRLGDLLKVGGTAAAVGGLGVVVPLAG
                     GFALGSLLGYSIEVSVFLAAALVATSVGITSGVLGSLGALRTMSGRVILGAAVIDDVL
                     AMLILAVATGLAGGGVSVDRILTLVALAVAFIGIVVIGGTQVLRRRRSLLTDPEFAST
                     PFLPGMIIMLGLAALSAIIGLAAIIGAFLAGMVVGESSERHALEEEVAPVAAFFTPFF
                     FGSIGAQVDLAGLADWGVIALLLGVTAVAIVTKFAGALVGSLRLGMRRATLIAAGMVP
                     RGEVGIVVAGLGLQTGAISGSVYSVVVGMAILTTLIVPPFLPALVRRAEADAEPPPEA
                     GGPPAGTEDDGGLPPEARGA"
     gene            complement(6557..7825)
                     /locus_tag="VFV59_09650"
     CDS             complement(6557..7825)
                     /locus_tag="VFV59_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEU5326132.1"
                     /translation="MTLPVLVGLAIFIGTYALIATERMHKTLAAMAGGVAMVLLGIVS
                     QEQAFAEIDFNVIFLLTGMMILAGIIRKTGVFGWVAVRAARFAGGEPYRILVVLSLIT
                     AIASAVLDNVTTVVLVGPITLFLAAQLSMNPFPFLMSEIMASNIGGASTLIGDPPNLL
                     IGSAASLDFTSFLLNLTPLAALILAVYLLAARWLYRDLLVVDPERRAGLLELNERDMI
                     THPALLRRSLVVLGLTLVGFLVHGFLHLEPATVALTGAVALMLVARESPDDVLRDVEW
                     PTLFFFIGLFMLIAGVIEIGVIEAVAGFIADVTGGALAATSFLLLWVSGVLSGIIDNI
                     PYTATMIPVVQQLSDGQQHDVLWWSLAIGADLGGNATIIGASANVILAGMAAREGHPI
                     TFGRFLRYGLPVTFGSMLIATVYVWLRYLL"
     gene            complement(7822..8376)
                     /locus_tag="VFV59_09655"
     CDS             complement(7822..8376)
                     /locus_tag="VFV59_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5326133.1"
                     /translation="MTDGDQRQTPESITQATTIEEADRILEMRPVVVRASASLAEVAE
                     RAVDNPGCRLIAVVGDDDRLVGVLPTHALVNEVFQKIVPEEFLGEITDLEAALDYASR
                     IGARTAADIMSPPVSVHSDETVRTAFERMHVSSYQGLPITDREEHVLGYVDQLELLVV
                     WVHATGREGLLQPTDRSTAQEPEA"
     gene            8558..10219
                     /locus_tag="VFV59_09660"
     CDS             8558..10219
                     /locus_tag="VFV59_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5326134.1"
                     /translation="MTSLGLTLLVALVAALVLGAVAHRLRLPPMLGYIVAGIVVGPAT
                     PGVVANRADMLALADIGVALLMFSIGLQFSIGELRAVGPRILAGAPVQVLLTMALGTG
                     TGLALGWPLVEALFVGGAVAVCSTVVLVKLAGETALHVTSYGRTAIGVSIVHDLVTIL
                     LVVLLTASVAPSEASPLSVATGVTVAIAFVAVVVFAGSRLLPRLLAIVAQLRSRELFV
                     ISVAVIAIGTAFAAQALGVSVALGAFVAGLALADSDLTASVLGEIVPLRELFASFFFV
                     SIGILLEPAAIVGAWPVALALVVIITLGKAIPVAALALFDGQRPGSAARAGALVGQSG
                     EFSFVLASVGLSAGAVQPDIFSLAMGAVVVSILLAQPLYAVAGRIGDWANELVGRPYL
                     PGDEPGSGLRRHAVILGYGRVGRAVSRVLQSRGFQWVAVDGEYAVAREARAAGAPVIY
                     GEAGTPSVLDQARIAECHALVVAIPDPLATRQAVRYALSRNPRIEIVARAHSEADEAE
                     LRRMGVARVVVAERELGTQLVRHALRRFGVSDREVAAIIEGTRRG"
     gene            complement(10430..11077)
                     /locus_tag="VFV59_09665"
     CDS             complement(10430..11077)
                     /locus_tag="VFV59_09665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5326135.1"
                     /translation="MESHGRWVCLDVGETLIDETRIWSLWADELGVPRLTFLAALGAV
                     IERGGEHRDVFPIFDAEDWQLRLAAIEGAYGGFGIPDLYPDALPAMAALTDAGYRVAV
                     VANQPASRAEELRRIGIQADVLAMSEGMGVAKPDPRFFAHALELMGSPPPEAVAYVGD
                     RVDNDVLPAIQAGMRAVWIRRGPWGVIQRLPEGTRPALVVDSLGELVERIGEAWE"
     gene            11252..13030
                     /locus_tag="VFV59_09670"
     CDS             11252..13030
                     /locus_tag="VFV59_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEU5326136.1"
                     /translation="MSRHRRLAIPVSTILAFATLLSATSAAIAKDEKPPVLRDRSEVE
                     VQILALNDFHGNLLPPSGSSGRVGPSGTGTTAGGAEYLATHVRNLEQTNANTIVVSAG
                     DLIGASPLLSALFHDEPTIEAMNQIGLDINAVGNHEFDEGTAELLRMQNGGCHPTDGC
                     QDGTGFAGADFEFLAANVVNESTGQTLFAPYTVRNFNGARVAFIGMTLEGTPNIVSPS
                     GVAGFDFLDEADTVNALIPQIRRSGVEAIVVLLHEGATPAGSVNQTSINQCGAGNAGI
                     SGAVVDIANRLDGAVDLLVSGHTHQPYTCTINGLPVTSAYSFGRLLTDIDMRISRATK
                     DVTSIEINNLIVTRDVPADQGITDLIAHYDTFADPIANRPIGHITADITRTNDDSLEQ
                     PMGNVIADAQLDATDDPGFGDAVVSFMNPGGVREALLYASSPEGEGDGVVTYGELFTV
                     QPFGNSLVTMTLTGAQIEQALEQQFCGLNSPANGFFYKVLLPSDGFHYTWDASAAGAA
                     SCVGADAVDPAGITLNGVPLDLAASYRVTVNSFLADGGDGFPALAQGTNRLGGAVDTD
                     AFEAYLAAAEPTGISPAPTDRIDVVP"
     gene            complement(13106..>13642)
                     /locus_tag="VFV59_09675"
     CDS             complement(13106..>13642)
                     /locus_tag="VFV59_09675"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEU5326137.1"
                     /translation="QVSQRIGADEAKTRTAIHAAVPALMAAFAGEAQRGGGIKTAIRQ
                     DHDGSIIDQLSEYLNGTAQLSPRTTNGAGILDHTLGGEQEQVARAVSAKSGLDLGTVM
                     KLLPLLAPIVMGMLGKKSNASPGGGFSLDDLGSILGRETEDAKQSNPDLGDILGRLGS
                     GGGIGDVLGGLLGGGRDR"
BASE COUNT         2064 a         4869 c         4723 g         1986 t
ORIGIN      
        1 cgttgtcgcg tgtggtctaa tcggcgcttc gggagggcca ccaggggacg accaccgacc
       61 agcggaggac gagcggacac ctcatggcaa tcgagaccga gcgcggcacg atgaccggcg
      121 acgagatcgt cgccctgacc aagcggcaca ccctgttcga gtggtccgcg cagagcaagg
      181 tcgacccgat cccggtcgcc ggcgcgaagg gcgtctactt ctggacgccc gaggggaagc
      241 ggttcctgga cttcaactcg cagctgatgt gcaccaacgt cggccacggc cacccgaagg
      301 tcgtcgaggc gatcaagcag caggccgaga cgctcgccta tgccaacccg ttcatggcca
      361 ccgagccccg cgcgcggctg gggcagaagc tggccgagct gacgcccggt gacatcgaca
      421 ccttcttctt caccaacggc ggcgccgagg ccaatgagaa cgccatcaag ctggcccgat
      481 tcgtcaccgg caaacacaag atcctggccc gctaccgcag ctaccacggt ggcaccgccg
      541 gcgccatcag cctgaccggc gacccgcgcc gctgggcggc ggagccgggc atcccgggcg
      601 tggtccacgt cctggacccg taccacggca tccagcgcgg ctgggactcg gccgacgagg
      661 cgctcgccaa cctcgaggag acgatccagc tcgagggccc gaataccatc gcggccttca
      721 tcctggagac cgtgacgggc acgaacggca tcctggtgcc gccggacggc tacctgcagg
      781 gcgtgcgcga gatctgcgat cgctacggga tcctgatgat cgccgacgag gtcatggccg
      841 gattcgggcg caccgggaag tggttcgcca tcgaccactg ggacgtggtg cccgacctca
      901 tcaccatggc gaagggcctg accagcgcgt acgtgcagct cggagcgctg ggcatgcggc
      961 gcaagtatgc ggagcacttc gagaacaacg tcttctacgg cggcctcacc tacaactcgc
     1021 acccgctggg ctgcgccacc gccctggcga cgatcgcggt gtacgaggag gagaacctca
     1081 tcgagcgcgc cgcacagatg ggcgaggtca tgcgcggcca catggaggag ctcgccgcga
     1141 agcatccgag cgtcggcgcg gtccgcaaca tcgggctgtt cgggatcgtg gagctggtcc
     1201 gcaaccgcga gacgatggag ccgatggcgc cgttcaacgg cacgtccgac gagatggcgg
     1261 cgctgggcaa gcggttccgc tccgacggcc tctacacgct cgtccgctgg aactatttct
     1321 tcaccaaccc gccgctgacc atcagcgagg aggagatggc cgaagggttc gcgatcatcg
     1381 atcgcgccct cgagatcacc gatgccgccg tcgcctgagc gcggctggct gcgacgggcg
     1441 ctcgtcttcg gtgtcttcct ggcgctgctg ctcctggtct gggaggggct gaagttcatc
     1501 ggcggcgatc cctggcgcac cgagacgtgg acctgggacc cgccgctgaa gatctggttc
     1561 gcgtccgacc tgctgctgcc gcacgtgtgg gacatcttcg gcgcgctctc gaacccggtg
     1621 cagcgcggca cggaccagtc gctggccggc ttcctggtcg gggcggcgtt ctacacctgg
     1681 cgtgaggcgg ccatcggctt cgtggtcggg gcggccatcg gcatcggcct ggcggcgctc
     1741 ttcgtccact cgcggctgct cgagcgggcg ctggtgccct acgtgatcgc cagccagacc
     1801 atccccatcg tcgcgctggc gccgatgatc gtcttcgcct tcggccagga ggtcatcagc
     1861 gtcgtcatca tcgcgaccta cctcaccttc ttcccggtga ccatcgccat gatccgcggc
     1921 ctgcgctcgc cggatccacg ggccatcgag ctgatgcgct cgtacgccgc aagccgctgg
     1981 agcgtcttct ggaagctccg catgccagcg tcgctcccct acctcttcac cgccctgaag
     2041 atcagcgcca ccgcctccat cgtcggggcg atcgtgggcg agggccccgg cggcatcccg
     2101 gacggcctcg gtcgcgcgat catcaacttc aacagccagt acgtcagcgg acccgagaag
     2161 ctgtgggcgg cgatcctcgc ctgcgcggtg gtcgggattg cgttcttcac catcgtccgg
     2221 gtcgcggagg tgctcacgct ccgcggccgc ggcatccgcc ccgaggtcgc ctgaggcctc
     2281 agccatggag ccaccgatga ccgattccgc cccgcgagcg tccgttgtcc gcattcgcgg
     2341 cgtcgacaag cagttcaccg ccggtgcggg cgccgtcacc gcgctgtccg gcatcgacct
     2401 ggggatcgag gctggcgagt tcgtcagcct catcggtcca tccggctgcg gcaagagcac
     2461 gctgctgcgg ctcatcggcg acctgacgcc gccgaccagc ggcagcgtcg aggtcaacgg
     2521 caagcccgcc catcaggcgc gcctcggtcg cgagtacggc atggtcttcc aggctccggt
     2581 cctcttcgag tggcgcacgg tggaggacaa cgtcaagctg ccgctcgagc tgctcgggat
     2641 ggaccgatcc gagcgcgacg cgcgcgctcg ggagatgctc tcgctcgtcg agctcggcga
     2701 cttcgcgcga caccacccgt accagctgtc gggcggtatg cagcagcgcg tggccatcgc
     2761 ccgtgcgctc gcgctgcagc ccgacatcct gctcatggac gagccgttcg gggcgctgga
     2821 cgagatgacc cgcgagcgca tgaactccga ggtgctgcgc atctggcggc agacgggcac
     2881 gaccgtcgtc ttcgtcaccc actccatccc ggaggcggtc ttcctttcga cccgggtcgt
     2941 cgtgatgtcg gcgcggccgg gccgcatcac gcgcgtcgtc gacgtgccgc tgccccagcc
     3001 gcgcaacgag gagacgcgcg aggacccgcg ctacttccgc gtcgtgaccg aggtccgcga
     3061 ggcgctgcgc gccggcggtg ggctgggcga ggaggcgccg ctggcgtccg aggagcgggt
     3121 ggcggccgag ggcggcatcg gatgaccgcc gcggcgccgt ccgtcggcgt cccggtccct
     3181 ccgcgaccgg gacggatgct ccggttcgtc cgcacgctgc gctactacgt gccggcgctg
     3241 gtcgtgttcg tgtcggtcat cgccgtctgg gaggtggcga cccgggcgct cgacgtccgg
     3301 gccttcatcc tgcccccacc gtcggccatc gggacggcgc tggtcgagaa ctggggcgcg
     3361 gggcgctggc cgctcttcga cgccgctcgc accaccttgc tggaggccgt cggcgggctc
     3421 ctcatcggca cgctggccgg cgtggtcgtc gccttcgcgg tcgggcgctg gatgaacgcg
     3481 agggatgcgg tgctgccgtt tgccgtggcc gccaacgcca ttcccatcat cgccatcgcc
     3541 ccgatcttca acgcctggtt cggcatcacc aacccggtgt cgaagatgat ggtggctgcg
     3601 ctgctcgtct tcttcccggt gatgatcaac gtgacgcgcg gcctcaccca ggtcgagcca
     3661 tcggccctgg agctgatgcg ctcctacgcg gcgtccgagt ggaccatcct gcgcaaggtc
     3721 cggatcccga acgcgctgcc ctacttcctc accgccctca aggtcgccac caccctgagc
     3781 ctcatcggcg ccatcgtggg cgaatacttc ggcggatcga ccagcgtcat cggccgggtc
     3841 gtggtccagt ccgcgtcgtc gctgcggttc gacgtgacct gggccgccat cctgctcggg
     3901 gcgatcgccg ggatcgtctt ctacctcgcc atcgtggcgc tggagcgggt gctgatcccg
     3961 tggcacgcct cggtccgggg cgaggagcac ggctgacggt gcacgggccg gcgaaaaaag
     4021 atgtggtgcg cagggcaccc catccgtaga atccaccgat cagtgcggga catcacggcc
     4081 cggacacacc acggaggagc ccatcggatg aagcgatccc ggatctggtt gagcctcgcc
     4141 atgagcgcgt cgctcgtcct ggcggcgtgc ggcacgcagt cgggcggaag cgccggtgcc
     4201 agcgcggacg agagcagcgc ggcgagtgac ggcgggggcg gcggcgagat gacatcggtc
     4261 cggctgcagc tgcagtgggc gccgcaggcc cagttcgccg gctacttcgc ggcccaggag
     4321 ctgggctact acgaggacga gggtctcgac gtccagatcc tggacggcgg cccggacgtc
     4381 atcccgcagc aggtcgggtc ggcggccgac ggccccgagt tcaccatcag ctgggtgccc
     4441 aaggtcctcg aggcccgtga ggccggctcc gacctggtcg acatcgcgca ggtcttccag
     4501 cgctccggca cgctgtcggt cgcctgggcc gactccggca tcagcggacc ggcggactac
     4561 gagggcaaga aggtcggcgt ctgggacttc ggcaacgagt gggaggtcat cgccgcggcc
     4621 aaggaggcgg gactcgagca gggcaccgac ttcgagcgcg tcatccagaa cttcgacatg
     4681 gtcgccttcc tgaaccgcga gatcgacgtc gcggaggcga tgatctacaa cgagtacgcg
     4741 caggtgctcg aggcggagaa cccggacacc ggcgagctgt accagccgga ggacctgacc
     4801 gtcatcgact acaacgaggt cgggacggcc atgctgcagg acgccatctt cgcccgggcc
     4861 agctggctcg ccgaggacgg caacgaggac gtcgccaccc gcttcctgcg ggcgagcttc
     4921 cgcggctgga tccactgccg ggagaacccc gacgactgcg tcgagttcac cgtcaacgcc
     4981 ggctccaccc tcggcatggg ccaccagcgc tggatgatga acgagatcaa tccgctgatc
     5041 tggccctcgc ccgatggcat cggcatgctc gacaccgatc tgtggcagca gacggtcgag
     5101 atcagcatgg actccgggat catcaccgcg gagccgggtg aggacgcgta ccgcaccgat
     5161 ctcgcccagg cggcgctgga cgggatcgag gaggacgcca ccggcgagag cttcaccaag
     5221 ggcagcgtcg aggtcacccc gggcggcgaa tagaccgatc cggacctcgc gacttcacgc
     5281 ccctccccgg ttcgccgggg aggggcttcc tcatgccccg cgcgcctcgg gcggcagccc
     5341 gccgtcgtcc tcggtcccgg cgggtgggcc tccggcctcc ggcggcggct ccgcatcggc
     5401 ctcggcgcgg cgcacgagcg ccggcaggaa gggcgggacg atgagcgtgg tgaggatcgc
     5461 catcccgacg accacgctgt agaccgagcc gctgatggcg ccggtctgca ggcccaggcc
     5521 ggcgaccacg atgcccacct cgccgcgcgg caccatgccg gccgcgatga gcgtggcccg
     5581 tcgcatgccc aggcgcaggg aacccaccag cgcgccggcg aacttggtga cgatcgcgac
     5641 cgccgtgaca ccgaggagca gcgcgatgac gccccagtcg gccaggccgg cgagatcgac
     5701 ctgtgcgccg atcgagccga agaagaacgg ggtgaagaag gccgcgaccg gcgccacctc
     5761 ctcctcgagc gcgtggcgct cgctcgactc gccgacgacc atgccggcca ggaaggcgcc
     5821 gatgatggcg gccaggccga tgatcgccga gagcgccgcc aggccgagca tgatgatcat
     5881 gccgggcagg aacggcgtgc tcgcgaactc cgggtcggtc agcagcgagc ggcgccgtcg
     5941 cagcacctgc gtcccgccga tgaccacgat gccgatgaac gcgacggcga gcgcgaccag
     6001 ggtcaggatg cggtccacgc tcacgccgcc gccagcgagc ccggtggcga cggccaggat
     6061 cagcatcgcc aggacgtcgt cgatgacggc ggcgccgagg atgacgcggc cggacatcgt
     6121 ccgcagcgca cccagcgatc ccagcacgcc ggaggtgatg ccgaccgagg tggcgaccag
     6181 cgccgcggcc aggaacacgc tgacctcgat cgagtagccg agcagcgaac cgagggcgaa
     6241 gccgccggcg agcgggacca ccacgcccag cccgcccacc gcggcggcgg tgccgccgac
     6301 cttgagcagg tcgcccaggc gcgtctcgac gccgacctgg aagaggagca gcacgacgcc
     6361 gatctcggcg aacagctgcg tctcggcgtt gatctcgtac cagcccagca gcgcgggtcc
     6421 cacgacgacg cccgccagga tctcgccgac caccgtcggc tgacccaggc gcttgaagat
     6481 ctcgtcaccg atcttggcag ccaggaggag gacgaagagc tggagaagga tctcggcgag
     6541 gtggtccatc ggtccctcag agcaggtagc ggagccagac gtagacggtg gcgatcagca
     6601 tcgagccgaa cgtgaccggc agtccgtagc gaaggaatcg gccgaacgtg atcgggtggc
     6661 cctcgcgcgc cgccatgccg gccaggatca cgttggcgct ggcgccgatg atcgtggcgt
     6721 tgccccccag gtcggccccg atggccagcg accaccacag cacgtcgtgc tgctgcccgt
     6781 cggacagctg ctgcacgacc gggatcatgg tcgcggtgta cgggatgttg tcgatgatcc
     6841 cggacaggac gccggacacc cacagcagca ggaacgacgt cgcggccagg gcgccaccgg
     6901 tcacgtcggc gatgaagccg gccaccgcct cgatgacgcc gatctcgatc acgccggcga
     6961 tcagcatgaa caggccgatg aagaagaaga gcgtgggcca ctcgacgtcg cgcagcacgt
     7021 catccggtga ctcacgcgcg acgagcatga gggccacggc gccggtcagc gccacggtcg
     7081 cgggctcgag gtgcaggaag ccgtggacca ggaagccgac gagcgtcagc cccagcacca
     7141 ccagggaacg ccgcaggagt gccggatgcg tgatcatgtc gcgctcgttg agctcgagca
     7201 ggccggcacg tcgctcgggg tccacgacca ggaggtcgcg gtacagccac cgcgcggcca
     7261 gcaggtagac cgccaggatc agcgcggcca gcggcgtcag gttgagcagg aacgacgtga
     7321 agtcgaggct cgccgcgctg ccgatgagca ggttcggcgg gtcgccgatg agggtcgatg
     7381 cgccgccgat attcgaggcc atgatctcgc tcatcaggaa cgggaagggg ttcatggaca
     7441 gctgggccgc aaggaagagg gtgatgggcc cgaccaggac gacggtcgtg acgttgtcca
     7501 gcacggcgga ggcgatggcc gtgatgaggc tgaggaccac caggatccga tacggctcac
     7561 cgccggcgaa ccgggccgcg cgcacggcga cccagccgaa gacgccggtc ttcctgatga
     7621 tcccggccag gatcatcatc ccggtcagca ggaagatgac gttgaagtcg atctccgcga
     7681 acgcctgctc ctgggagacg atgccgagca gcaccatggc cacgccaccg gccatcgccg
     7741 ccagcgtctt gtgcatccgc tccgtcgcga tcagcgcgta cgtgccgatg aagatcgcca
     7801 gcccgaccag gacggggagg gtcacgcctc cggctcctgc gcggtcgatc gatcggtcgg
     7861 ctggagcagg ccctcgcgcc cggtggcgtg cacccacacc accagcagct ccagctggtc
     7921 cacgtacccg aggacgtgct cctcgcgatc ggtgatgggc aggccctggt agctgctgac
     7981 gtgcatccgc tcgaaggccg tgcgcaccgt ctcgtccgag tgcacgctga ccggcggcga
     8041 catgatgtcg gccgcggtgc gtgcgccgat gcgcgatgcg tagtcgaggg cggcctccag
     8101 gtccgtgatc tcgcccaaga actcctcggg cacgatcttc tggaagacct cgttgacgag
     8161 tgcatgggtc ggcaggacac ccaccagccg atcgtcgtcc ccgaccacgg cgatcaggcg
     8221 gcagcccgga ttgtcgaccg cgcgctccgc gacctcggcc aggctggcgg aggcgcggac
     8281 cacgaccggc cgcatctcga ggatgcgatc cgcctcctcg atggtggtcg cctgggtgat
     8341 cgattcaggg gtctgccgct gatcgccgtc ggtcatcagt tcgctccacg cagcacagga
     8401 ggccacgcca ttgcgcagcc tccacctcgt gcgccgtggg tacggatgcg gcgctcaccc
     8461 gctgagcggc gcccctccgg acggccgatc ggcccgccaa gtctatccga cgccgccccg
     8521 cccgtatgat cggcggcatg gcgcccaggg tccgacgatg accagcctcg gcctcaccct
     8581 gctcgtggcg ctcgtcgccg cgctggtgct gggcgccgtg gcgcatcggc tccggctgcc
     8641 gccgatgctc ggctacatcg tcgcgggcat cgtggtcggg ccggcgaccc ccggtgtcgt
     8701 ggccaaccgc gccgacatgc tggccttggc cgacatcggt gtcgcgctgc tgatgttcag
     8761 catcggcctg cagttcagca tcggcgagct gcgcgccgtc gggcctcgca tcctggccgg
     8821 agcaccggtc caggtgctcc tcaccatggc gctcggcacc ggaaccggac tggcgctcgg
     8881 ctggccgctg gtcgaggcgc tgttcgtcgg cggagcggtg gcggtgtgct cgaccgtggt
     8941 gctggtgaag ctcgccggcg agaccgccct ccacgtgacc agttacgggc gcacggccat
     9001 cggcgtgtcg atcgtgcacg acctcgtcac gatcctgctc gtcgtcctcc tgacggcgag
     9061 cgtggcgccg tccgaggcgt ccccgctgtc cgtggcgacc ggcgtgacgg tcgcgatcgc
     9121 cttcgtcgcc gtggtggtct tcgcgggctc gcggctgctg ccgcggctgc tggccatcgt
     9181 cgcccagctc cggtcccgcg agctgttcgt catcagcgtg gcggtcatcg ccatcggcac
     9241 cgccttcgcc gcccaggcgc tcggtgtcag cgtcgcgctg ggggccttcg tcgccgggct
     9301 ggccctcgcc gactccgacc tcaccgccag cgtcctgggc gagatcgtcc cgctccgaga
     9361 gctgttcgcg tcgttcttct tcgtctccat cggcatcctg ctcgagccgg ccgccatcgt
     9421 cggggcgtgg cccgtggcgc tcgcgctggt ggtgatcatc acgctcggca aggccatccc
     9481 cgtcgccgca ctggcgttgt tcgacgggca gcgcccggga tcggccgcgc gggcgggggc
     9541 actggtgggg cagagcgggg agttcagctt cgtgctcgcc tcggtcggcc tcagcgccgg
     9601 cgccgtgcag ccggacatct tcagcctggc gatgggcgcg gtggtcgtct cgatcctgct
     9661 cgcccagccg ctgtatgcgg tggcgggccg catcggcgac tgggcgaacg agctggtcgg
     9721 tcggccctac ctgcccggag acgagccggg cagcgggctc cgtcgccacg cggtgatcct
     9781 cggctacggg cgtgtcggcc gtgccgtctc acgcgtcctc cagtcgcgcg ggttccagtg
     9841 ggtcgccgtc gacggcgagt acgccgtggc acgggaggcg cgcgcggccg gtgcgccggt
     9901 gatctacggc gaggcgggca cgccgtcggt gctcgaccag gcgcggatcg ccgagtgcca
     9961 tgccctcgtg gtcgcgatcc cggatccgtt ggccacccgg caggcggtgc gctacgcgct
    10021 gtcgcggaac ccgcgcatcg agatcgtggc gcgcgcgcac tccgaggcgg acgaggccga
    10081 gctgcgccgc atgggcgtcg cccgggtcgt ggtcgccgag cgcgagctcg ggacgcagct
    10141 ggtgcgccac gccctccgcc gcttcggcgt cagcgaccgc gaggtggccg ccatcatcga
    10201 gggcacgcgg cgcggatagc gcgccgcggc gtctcgatca atcgaggcga cagcgcgcca
    10261 gcctggtcac gtgccgggtt ctcgcgattg agcgcggatg ccggagcgcg acggacccga
    10321 tcaatcggcg atcgtgtggg gcacgtcgcg atccatcgcg acacccgacg cgaaccagcc
    10381 cgcccgcgcg gaaccgtcgc gatcgatcgc gacggacgcg cagacactcc tactcccagg
    10441 cctcgccgat gcgctcgacc agctcgccga gcgagtcgac gaccagcgcc ggtcgcgtgc
    10501 cctcgggcag gcgctggatg acgccccatg ggccccgccg gatccacacg gcgcgcatcc
    10561 ccgcctggat ggccggcagc acgtcgttgt ccacgcgatc gccgacgtac gccacggcct
    10621 cgggcggggg cgatcccatc agctcgagcg cgtgcgcgaa gaagcgcggg tccggcttgg
    10681 cgacgcccat gccctcgctc atggccagca cgtccgcctg gatgccgatg cgccgcagct
    10741 cctcggcccg gctcgcgggc tggttggcga ccactgccac ccggtagccc gcatcggtca
    10801 gcgccgccat cgccggcagc gcgtcgggat acaggtccgg gatgccgaag ccgccgtacg
    10861 cgccctcgat cgccgcgagg cgcagctgcc agtcctcggc gtcgaaaatc gggaagacgt
    10921 cgcggtgctc gccgccgcgc tcaatgacgg cgccgagggc cgccaggaag gtcagccgcg
    10981 ggacgcccag ctcatcggcc cagagcgacc agatgcgggt ctcgtcgatg agggtctccc
    11041 cgacgtccag gcacacccac cggccgtgcg actccatgcg tcgatgctag ccgggcgcgc
    11101 cgatggtcgt gccgacggcg cgcgcagtgg tagcctgtcc cggtcgtcgc gtgcccttcg
    11161 gctctactgg tctgcgcctc ggacgtgaag gctcaccgcc atcgcgaccg gcggcgcggt
    11221 ccgccaccga gcaacgagct gagggaacca gatgagcaga caccgacgac tcgccattcc
    11281 cgtttcgacc atcctcgcgt tcgcgacgct gctgagcgcg acgtcggccg ccatcgccaa
    11341 ggacgagaaa ccgcccgtcc tgcgcgaccg atcggaggtc gaggtgcaga tcctcgccct
    11401 caacgacttc cacgggaacc tgctgccccc gagcggctcg agcggccgcg tcggtccgtc
    11461 cggcaccggg accaccgccg gcggcgccga gtacctcgcg acgcacgtcc gcaacctcga
    11521 gcagacgaac gcgaacacca tcgtggtgtc cgccggtgac ctcatcgggg ccagcccgct
    11581 gctgtccgcc ctgttccacg acgagccgac catcgaggcg atgaaccaga tcggcctcga
    11641 catcaacgcc gtgggcaacc atgagttcga cgagggcacg gccgagctgc tgcgcatgca
    11701 gaacggcggc tgccacccga ccgacggctg ccaggacggc accggcttcg ccggcgcgga
    11761 cttcgagttc ctcgccgcga acgtcgtgaa cgagtcgacc gggcagacgc tcttcgcgcc
    11821 ctacacggtc cggaacttca acggcgcgcg cgtcgcgttc atcggcatga ccctggaggg
    11881 gacgccgaac atcgtcagcc cgtccggcgt ggccggcttc gacttcctcg acgaggcaga
    11941 caccgtcaac gccctgatcc cgcagatccg tcggagcggc gtcgaagcga tcgtggtgct
    12001 gctgcacgag ggagccaccc cggccggctc ggtgaaccag acctcgatca accagtgcgg
    12061 tgcaggcaac gccggaatca gcggggcggt cgtggacatc gccaaccgcc tcgacggcgc
    12121 ggtggacctc ctcgtctcgg gccacacgca ccagccgtac acctgcacga tcaacggcct
    12181 gcccgtgacg agcgcttact cgttcggccg gctgctgacc gacatcgaca tgcgcatcag
    12241 ccgggccacc aaggacgtca cctcgatcga gatcaacaac ctgatcgtca cccgtgacgt
    12301 tccggccgac cagggcatca ccgacctgat cgcgcactac gacacgttcg ccgatccgat
    12361 cgccaaccgc cccatcgggc acatcacggc cgacatcacc cgcacgaacg acgactcgct
    12421 cgagcagccg atgggcaacg tcatcgcgga cgctcagctg gacgctaccg acgacccggg
    12481 cttcggcgac gcggtggtga gcttcatgaa ccccggcggc gtccgagagg ccctgctcta
    12541 cgcgagcagc cccgagggcg agggcgacgg cgtcgtgacc tacggcgagc tgttcacggt
    12601 ccagccgttc ggcaactcgc tcgtgacgat gacgctgacc ggcgcgcaga tcgaacaggc
    12661 gctcgaacag cagttctgcg gtctgaactc gccggccaac gggttcttct acaaggtgct
    12721 cctgccctcc gatgggttcc actacacgtg ggatgcctcg gccgccggag cagccagctg
    12781 cgtcggtgcc gatgcggtcg atcccgcggg catcaccctg aacggcgtgc cgctcgacct
    12841 cgcggcgagc taccgggtga cggtgaacag cttcctcgcc gatggcgggg acgggttccc
    12901 cgcgctcgcg cagggcacca accggctggg tggcgccgtg gacaccgatg cgttcgaggc
    12961 ctacctcgcc gcggcggagc cgaccggcat ctccccggct cccaccgacc ggatcgacgt
    13021 ggtcccctag gcaactgcgg acgcccccgc aacggaacgg gccgcctcgg cgaggcggcc
    13081 cgtttcctgg tgtcggcgct tcgcgctagc ggtcgcgacc tccgccgagg agcccgccga
    13141 gcacgtcgcc gatgcctccg ccgctcccca gccggcccag gatgtcgccg aggtccgggt
    13201 tgctctgctt cgcatcctcg gtctcgcgtc ccaggatgct gcccaggtcg tcgaggctga
    13261 agccgccccc gggcgatgcg ttgctcttct tgccgagcat gcccatcacg atcggcgcga
    13321 gaaggggcag gagcttcatg accgtgccaa ggtcgaggcc gctcttggcg ctcaccgcgc
    13381 gagcgacctg ttcctgctcg ccgcccaggg tgtggtcgag gatgccggcg ccgttcgtcg
    13441 ttcgcggcga cagctgtgcc gtgccgttca ggtactcgct gagctggtcg atgatcgacc
    13501 catcgtggtc ctggcggatg gccgtcttga tgccgccgcc tcgctgggcc tcgcccgcga
    13561 acgcggccat cagcgccggg acggcggcgt ggatcgcggt ccgcgtcttg gcctcatcgg
    13621 caccgatgcg ctggctgacc tg
//
LOCUS       DASTYP010000221         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_531945, whole genome shotgun sequence.
ACCESSION   DASTYP010000221 DASTYP010000000
VERSION     DASTYP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531945"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1708
                     /locus_tag="VFV59_09680"
     CDS             143..1708
                     /locus_tag="VFV59_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5326138.1"
                     /translation="MPPRATRREWIGLAVLTLAALVYAMDLTVLNLAIPIISADLRPS
                     SVQLLWMIDIYGFLVAGLLITMGTLGDRIGRRRLLLGGAAGFALASLLAAFSTSSEML
                     IASRAVMGVAGATVAPSTLSLIFTMFLDPKQRSTAIGVWIAAYSAGGAIGPVLGGILL
                     EWFWWGSVFLIGVPVMALLLLLGPRTLPEYRDPNARRLDLPSAAMSIFAILGGVFGLK
                     QIAQDGVSTLAVLAIVAGVAVGLAFVRRQLVLPSPVIDVRLFRIRAFSASLGTYFFGI
                     FVVVGYFLFISQYLQLVLRLSPIQAAFWSLPSAAGFIVGSTVAPKIIHRYRPSVIMGA
                     GMLIAAVGTAMLLGLTVDGAGSLPLIAGASVVISLGLAPVITLATELIVGSAPPEQAG
                     AATGMSETSGELGGALGIAILGSIGTAVYRAEVADTVPHGVPAAAVDAARDTLGGALA
                     IAESLPAALGDALRAAAQVAFVDALHVVAAVAVLGAAVTAIGAAVALWTVSPRTKQAA
                     EEPGSPLPVADRA"
     gene            complement(1807..2508)
                     /locus_tag="VFV59_09685"
     CDS             complement(1807..2508)
                     /locus_tag="VFV59_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003918879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEU5326139.1"
                     /translation="MRIVVVGGTGRIGARVVTLLRDQGHDVAAASRSTGVDALSGTGL
                     AEALSGAEVLVDVTNPRAPAPASPMEFFTVGTSNLVEAALRAGIGHYVVLSIVGIDLV
                     PDSAYYRAKVVQEREVTASSLPSSIVRATQFVEFTDAIVDSMTDGREVRVPDAVIQPI
                     PADDVATTLARVATGSPTDTILEIAGPSILTFEQMAREVLIRRGDATLRVVVDSAARY
                     FGAQLASRSLIPQPS"
     gene            2541..>2702
                     /locus_tag="VFV59_09690"
     CDS             2541..>2702
                     /locus_tag="VFV59_09690"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5326140.1"
                     /translation="MDERPRTDTDLAALLEATERRRLRALVDADLPTADALHADDYEL
                     ITPGGSSLSK"
BASE COUNT          362 a          986 c          929 g          425 t
ORIGIN      
        1 cgcgagccgt acgggttcga cgaggccccc gggtccctga tcgcgaccct tgccgatccg
       61 gacgacaact acttccagct ggtgagcccg atgggtcccg ccgacgcctg aagtgatgca
      121 ggagacgagg gaagcggcgg cggtgccacc gcgcgcgacg aggcgggagt ggatcgggct
      181 ggcggtcctc accttggcgg ccctggtcta cgccatggac ctcaccgtcc tcaacctggc
      241 catcccgatc atcagcgcgg acctgcggcc ttcgagcgtg cagctgctgt ggatgatcga
      301 catctacggc ttcctggtcg ccgggctgct catcaccatg gggaccctgg gcgaccggat
      361 cggccgtcgg cggctgctgc tcggcggggc ggccgggttc gcgctcgcgt cgctgctcgc
      421 ggccttctcg acgagctcgg agatgctcat cgccagccgc gcggtgatgg gcgtcgcggg
      481 cgccaccgtc gcgccgtcga cgctgtcgct catcttcacc atgttcctcg acccgaagca
      541 gcgctcgacc gccatcggcg tctggatcgc ggcctactcc gcgggtggcg cgatcgggcc
      601 cgtgctcggc gggatcctgc tggagtggtt ctggtggggc tcggtcttcc tcatcggcgt
      661 gccggtgatg gcgctgctcc tcctgctcgg tccacggacc ctgcccgagt accgcgaccc
      721 gaacgcccgg cgcctggacc tccccagcgc cgcgatgtcg atcttcgcca tcctcggcgg
      781 agtcttcggt ctgaagcaga tcgcccagga cggagtctcg acgctggcgg tcctggccat
      841 cgtcgccggg gtggcggtgg gcctcgcctt cgtgcggcgc cagctcgtgc tgccgtctcc
      901 ggtcatcgac gtgcgcctct tccggatccg cgcgttcagc gcgtcgctcg gcacctactt
      961 cttcgggatc ttcgtggtgg tcgggtactt cctgttcatc tcgcagtacc tgcagctggt
     1021 cctgcggctg tcacccatcc aggccgcctt ctggtcgctg ccctcggccg cgggcttcat
     1081 cgtgggctcg acggtcgcac cgaagatcat ccaccgctac cggccttcgg tgatcatggg
     1141 cgccggcatg ctcatcgcgg ccgtcgggac ggccatgctg ctgggactca ccgtcgacgg
     1201 tgctggaagc ctgccactca tcgccggcgc atccgtcgtc atctccctcg gcctggcccc
     1261 ggtgatcacg ctcgccacgg agctgatcgt gggaagcgcg ccgccggagc aggcgggggc
     1321 cgcgaccggc atgtctgaga cgagcggcga gctcggcggt gccctgggca tcgcgatcct
     1381 gggcagtatc ggcaccgccg tctaccgcgc cgaggtcgcc gacacggtgc cgcacggcgt
     1441 gccggccgcg gccgttgatg ccgctcgtga cacgctgggc ggcgcgctgg cgatcgcgga
     1501 gagcctgccc gccgcgctgg gggacgcgct ccgggcagcg gcccaggtcg cgttcgtcga
     1561 cgccctgcac gtcgtcgccg ccgtcgcggt tttgggggcc gcggtcaccg ccatcggcgc
     1621 cgccgtcgcg ctgtggacgg tgtcgccgcg cacaaagcag gcggccgagg agccggggtc
     1681 gccgcttccg gtcgctgatc gagcctgagg cgtcagccca ggacgtctcc gcccagccgc
     1741 gcgacgccca tcggatccgc gatggcctcg gcagcctggc gaccgtcagc ggcccaccgc
     1801 gtccggctac gacggttgcg gaatgaggct gcgagaggcc aattgcgcac cgaagtagcg
     1861 ggccgccgag tccacgacga cccggagcgt cgcatcgccc cgccggatca gcacctcgcg
     1921 agccatctgc tcgaaggtga ggatgctcgg tccggcgatc tccaggatcg tgtcggtggg
     1981 cgaacccgtc gccacgcgcg cgagggtggt cgcgacatcg tcggccggaa tcggctggat
     2041 cacggcatcc ggcacacgaa cctcgcgccc gtccgtcatc gagtcgacga tcgcatcggt
     2101 gaactcgacg aactgcgtcg cgcgaacgat cgaggagggg agcgacgacg ccgtcacctc
     2161 gcgctcctgc acgaccttgg cccggtagta ggcgctgtcg gggaccagat cgatgcccac
     2221 gatcgacagc accacgtagt ggccgatgcc ggcgcgcagc gccgcctcca ccaggttcga
     2281 ggtcccgacg gtgaagaact ccatcggcga tgccggcgcg ggcgcccggg gattcgtcac
     2341 gtccacgagc acctcggctc cggagagcgc ctcagccagg ccggtcccgg agagcgcatc
     2401 tacaccggtc gagcgcgacg cggccgcgac atcgtgaccc tggtcgcgca ggagggtgac
     2461 gacccgcgca ccgatgcgcc ccgttccgcc gacaaccacg atcctcacga cgacgaccat
     2521 accaacgcta ggatcagcgc gtggacgagc gccccaggac cgatacggat ctcgccgcac
     2581 tcctcgaggc caccgagcgc cgtcggctcc gggcactggt cgacgccgac ctccccacgg
     2641 ccgatgcgct ccatgccgat gactacgagc tcatcacgcc tggcggcagc tccctgtcga
     2701 ag
//
LOCUS       DASTYP010000222         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_678433, whole genome shotgun sequence.
ACCESSION   DASTYP010000222 DASTYP010000000
VERSION     DASTYP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678433"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFV59_09695"
     CDS             <1..292
                     /locus_tag="VFV59_09695"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEU5326141.1"
                     /translation="PRRFLDAMIELDADALCALDPSCDLEGIRAQLEMVRAVAAGTLE
                     RAPFATMTPSQRFHWLSTPRSTVVQPGPLHAGTTDDPAGTLAHLFETVVRRR"
     gene            289..1104
                     /locus_tag="VFV59_09700"
     CDS             289..1104
                     /locus_tag="VFV59_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326142.1"
                     /translation="MTERRWLLVVAVTGIALFGLSFLDAWILQVREVRGEGYRWVETS
                     YGAWRGIGMPVLTLAALLALVVGVAALARLSGRWWTPDLLLLAGSSAALGLVLASAWP
                     VTHRAHTTTVDLTPSWLLPVGALLGLLMGAGSIAVSRVGRRAVLSAAVLVLGVVAMGA
                     VGRWQLLAASEGTGRHWTEGRYTRAGTADQPTETLVLTETTYAVEGRWSGELEWSGWT
                     IIFDHDPACPGSRGTYHARDVGEEDIRFVKVVDTCLDGERAADLETGIWRRDP"
     gene            complement(1117..1674)
                     /locus_tag="VFV59_09705"
     CDS             complement(1117..1674)
                     /locus_tag="VFV59_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5326143.1"
                     /translation="MSTTLDPHATIRPLLRTRQVREFTDEPVDPAAVDALADAARWSG
                     SSQNTQPWRFVVLRKSETLARLAELGAPHTRSLRTAAAAVAIVLPAEPGRAVSHAFDE
                     GRAAERILVAASMLGLAAGIGWVRSEVRPAVGELLGLPEDRFVRTIVAIGRPTPQARK
                     PKKPRGEARLPREDTVFEERWPEPR"
     gene            complement(1732..2502)
                     /locus_tag="VFV59_09710"
     CDS             complement(1732..2502)
                     /locus_tag="VFV59_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5326144.1"
                     /translation="MSLGWWLSDTAQMIRRNLLHIRRTPELLLDVTVSPIMFVLLFTL
                     VFGGAIDVRTATYTNYLMAGIFVQTIAFAGVYTGVLLANDLKNGMIDRFRSLPMSQSA
                     VLTGRTLTDLLRALLAVLIMWIVGLLVGFRPAGGVVGTIGAIGLLLLFGYALSWLGVA
                     MGVAVRTPEALQGVIFAAVFPLTFVSSAFVPTDTMPDWLAWFADRQPMTLVINAVRSL
                     TLDGSGGPSFWPAVLWSAGILAVCFPLGMWLYRRRTTD"
     gene            complement(2499..3452)
                     /locus_tag="VFV59_09715"
     CDS             complement(2499..3452)
                     /locus_tag="VFV59_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018519091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5326145.1"
                     /translation="MIEAAGLHKSFGETHALRGLDLRADEGQILGVLGPNGAGKTTAV
                     RILTTLSRPDRGSATVAGVDVLRHPQEVRRRIGVAGQYASLDELLTGRENLRMIGRLL
                     RLDGRTAATRADELIDRFELTDAADRTVKTYSGGMRRRLDLAAALIAQPPVIFLDEPT
                     TGLDPKGRLSMWELIGELARGGTTVLMTTQYLEEADRLADDIVVIDHGRAIATGTALQ
                     LKQQVGGERLEVRIAEGSPLDTARSVLGAIGEGSVHVEADGRQLSVPVTSHDGVLTNA
                     IRELDRAGVRVDDIGFRRPTLDEVFLSLTGRPAEEPLEVTA"
BASE COUNT          481 a         1250 c         1308 g          485 t
ORIGIN      
        1 gccgcgacgg ttcctggacg cgatgatcga gctcgatgcc gatgcgctgt gcgcgctgga
       61 cccctcgtgc gacctcgagg gcatccgcgc ccagctcgag atggtccgcg ccgtcgcggc
      121 cggcaccctg gagcgtgcac cgttcgccac gatgacgccg tcgcagcgct tccactggct
      181 cagcacgccg cgcagcacgg tcgttcagcc cgggccgctg cacgccggga ccaccgatga
      241 cccggcgggc accctcgcgc acctgttcga gaccgtcgtc cgccgccgat gaccgagcga
      301 cgctggctgc tcgtggtggc cgtcaccggc atcgccctct tcgggctgtc gttcctggac
      361 gcgtggatcc tccaggtccg cgaggtccgc ggcgagggct accgctgggt ggagacgtcg
      421 tacggcgcgt ggcgcgggat cggcatgccg gtgctcaccc tggccgcgct gctggcgctg
      481 gtggtcgggg tcgccgcgct cgcgcggctg agtggacgat ggtggacgcc ggacctcctc
      541 ctgctcgccg gctcgtccgc ggccctgggc ctcgtgctgg cgagcgcgtg gccggtgacg
      601 catcgggctc acacgacgac cgtggatctg acgccgtcct ggctgctgcc cgtcggcgcg
      661 ctgctggggc tcctgatggg ggcggggtcc atcgcggtgt cgcgcgtcgg gcggcgcgcg
      721 gtgctgtccg ccgccgtgct ggtcttgggc gtggtcgcca tgggcgcggt ggggcgctgg
      781 cagctgctgg cggccagcga ggggaccggc cggcactgga ccgagggccg ctacacgcgt
      841 gcagggaccg cggaccagcc gaccgagacg ctggtcctga ccgagacgac ctacgcggtc
      901 gagggtcgct ggtcgggcga gctggagtgg tccggctgga ccatcatctt cgaccacgac
      961 cccgcctgcc cgggatcacg agggacctac cacgcgcgcg acgtcggcga ggaggacatc
     1021 cggttcgtca aggtcgtcga cacgtgcctc gacggcgagc gcgccgccga cctcgagacg
     1081 gggatctggc gccgggatcc ctgacgccgt cggtcgtcag cgtggctccg gccaacgctc
     1141 ctcgaagacc gtgtcctcgc gcggcagtcg cgcctcgccc cggggcttct tcggctttcg
     1201 cgcctgcggc gtcgggcggc cgatcgccac gatggtgcgc acgaagcgat cctccggcag
     1261 gccgagcagc tcgcccaccg cgggccggac ctcggagcga acccagccga tgcccgcggc
     1321 caggcccagc atcgaggctg cgaccaggat ccgctccgcg gcgcgtccct cgtcgaaggc
     1381 gtgggagacg gccctgcccg gctcggccgg cagcacgatc gcgacggccg ccgcagccgt
     1441 gcgcagggac cgcgtgtggg gcgcgccgag ctccgcgagc cgagcgaggg tctcggactt
     1501 ccgcaggacg acgaagcgcc acggctgcgt gttctggctc gagccggacc agcgggcggc
     1561 atcggcgagg gcgtcgaccg ccgccgggtc gaccggctcg tcggtgaact cgcggacctg
     1621 gcgcgttcgg agcaggggac ggatggtcgc gtgcggatcg agggtcgtgg acatggccca
     1681 agggtaggcc ggaggcgctg cgcgcgccgg cctgagccgg cgcatcggtc gtcagtcggt
     1741 cgtgcgtcgc cggtagagcc acatccccag cgggaagcag acggccagga tcccggcgga
     1801 ccacaggacg gccggccaga acgacgggcc acccgacccg tccagggtca ggctgcggac
     1861 cgcgttgatg accagggtca tcggctggcg atcggcgaac caggccagcc agtccggcat
     1921 ggtgtcggtc gggacgaagg cgctgctcac gaaggtcagc gggaagaccg ccgcgaagat
     1981 cacgccctgc agcgcctccg gcgtgcggac cgcgacgccc atcgccacgc cgagccacga
     2041 cagcgcgtag ccgaagagga gcaggaggcc gatggcaccg atggtgccca ccacgccccc
     2101 ggcgggccgg aacccgacca gcaggccgac gatccacatg atcaggacgg ccagcagcgc
     2161 gcgcaggaga tccgtcagcg tgcgaccggt caggacggcc gactgcgaca tcggcagcga
     2221 gcggaaccga tcgatcatcc cgttcttcag gtcgttggcg agcagcacgc ccgtgtagac
     2281 ccccgcgaac gcgatggtct gcacgaagat gccggccatc aggtagttcg tgtacgtcgc
     2341 cgtccggacg tcgatggcgc cgccgaagac gagggtgaag agcagcacga acatgatcgg
     2401 cgagaccgtc acatccagca gcagctccgg cgtgcgacgg atgtggagca ggttgcgccg
     2461 gatcatctgg gcggtgtcgc tcagccacca tccgaggctc atgcggtcac ctccaggggc
     2521 tcctcggccg gtcgcccggt cagcgacagg aacacctcgt ccagcgtcgg tcgccggaag
     2581 ccgatgtcgt cgacgcgaac gccggcgcga tccagctcgc gaatggcgtt ggtcagcacg
     2641 ccgtcgtgcg acgtcaccgg gacgctcagc tggcgcccgt cggcctcgac gtggacgctg
     2701 ccctcaccga tggcgccgag cacgctccgg gcggtgtcga gcggcgagcc ctcggcgatg
     2761 cgcacctcga gacgctcgcc gccgacctgc tgcttcagct ggagcgcggt gccggtggcg
     2821 atcgcccggc cgtggtcgat gacgacgata tcgtcggcca gccgatccgc ctcctccagg
     2881 tactgcgtgg tcatgagcac cgtcgtgccg ccgcgtgcca gctcgccgat gagctcccac
     2941 atgctcagcc ggcccttcgg atccaggccg gtggtcggct cgtccaggaa gatgaccggc
     3001 ggctgggcga tcagcgccgc cgccaggtcg agtcgccgcc gcatgccgcc ggagtacgtc
     3061 ttgaccgtgc ggtcggcggc atcggtcagc tcgaatcggt cgatcagctc gtcggcgcgc
     3121 gtcgccgcgg tgcggccgtc caggcgcagc agccggccga tcatgcgcag gttctcgcgg
     3181 ccggtgagca gctcgtccag cgacgcgtac tgcccggcca cgccgattcg gcgccgaacc
     3241 tcttgcggat gtcgcagcac gtcaacgccc gcgacggtcg cggagccgcg gtcgggacgg
     3301 ctgagcgttg tgaggatccg gacggcggtc gtcttgccgg ccccgttcgg ccccagcacg
     3361 cccaggatct ggccctcgtc ggcgcgcagg tcgagaccgc gcagggcatg ggtctcgccg
     3421 aaggacttgt ggaggccggc ggcctcgatc atggtgcggc tcaaggccgg cctcgtgctc
     3481 ggtgggggcc ggaatgatac cgcggctcgg ccgcccggtc ctgc
//
LOCUS       DASTYP010000223        11889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1371308, whole genome shotgun sequence.
ACCESSION   DASTYP010000223 DASTYP010000000
VERSION     DASTYP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11889
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371308"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFV59_09720"
     CDS             complement(<1..453)
                     /locus_tag="VFV59_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5326146.1"
                     /translation="MRRIGAALVSLAAFLVAWKMVTVIGGYPEFILPAPEAVVERAVR
                     ALSTGVLWEHAAVTLLEIVLGFAAGGTAAVLTGIALGKSVLVERVLSPYIVAAQAVPI
                     LALAPLLDIWFGGGLLARVVICALIVFFPIAIATMVGIRSADPLLDEML"
     gene            complement(450..1274)
                     /locus_tag="VFV59_09725"
     CDS             complement(450..1274)
                     /locus_tag="VFV59_09725"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5326147.1"
                     /translation="MGARTTSAGLDVRLDGVGVRFGEADSGLQALDGLTLEVPPGSFT
                     AIIGPNGCGKSTLLRLVAGLLGPTAGTVRVGGAEPRAGDGRVALAFQQPRLVPWRTTL
                     ANVALPLELDGLAPSDRQERAQLALERVGLAGAARLRPGELSGGMAQRAALARALITD
                     PPVLLLDEPFSALDALTRETFDTELQRLWMEQPRTVVLVTHSVVEAVALADRVVVMTP
                     RPGRVARIVEVDLERPRAPQLLGEPRAAELAADVRAGLAEGHALELRPWAEGEGAA"
     gene            complement(1571..2194)
                     /locus_tag="VFV59_09730"
     CDS             complement(1571..2194)
                     /locus_tag="VFV59_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU5326148.1"
                     /translation="MYDAVGYVGVALAVALETIVAPIPSEVILPMAGWKISQSATDPN
                     VLEPLTGLPWNFPLAILLATAGSLVGAVVGYYIGAWGGRPILDRYGKYVGIGADDLDR
                     ADRWFERWGSWAVFFGRMVPLVRTFVSYPAGISRMPMGRFLLFSALGSLPWNAALIFA
                     GFVVGENYPQIEATLKPYEYLIYVVVIGIVGYFVVRWWLGKRRAAAG"
     gene            complement(2243..3121)
                     /locus_tag="VFV59_09735"
     CDS             complement(2243..3121)
                     /locus_tag="VFV59_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5326149.1"
                     /translation="MRRLPDPTSRLVVEDDAALRLGGAVPSPAVAFLLSVIALPALFW
                     LVMLKRTTVYTAPEEWFMSNVVPAFHSWVAANLPPVEVLIIAISALGSGWFVALAFAA
                     LGLLALAKNRRDLAFLLALGTLCFPMEWALKYFTSAEAISIGDLVSAMFNVNNIGLDD
                     IADFPAGHALRATALYGLAAFCVARLAHDRRQGEIAYAVALVLIGAISATRIYLGAHY
                     PMDVLGGWMAGGALVAILVAVHVLRVDERARLREAAMRSGAGQRRVAVPRRTAPAAPD
                     AGAPASNTSSGEPVGG"
     gene            3142..4677
                     /gene="hutH"
                     /locus_tag="VFV59_09740"
     CDS             3142..4677
                     /gene="hutH"
                     /locus_tag="VFV59_09740"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU5326150.1"
                     /translation="MPPLLLTGRDLTIDNVVEVARGRRRVELTPEAADRMRASRAVIE
                     RLVADGGTVYGVTTGFGDLADVRIEPDQTADLQHNLVRSHAAGVGEPLPDDVVRAMLL
                     LRANALAVGLSGVRVEVVELLLGMLNAAVHPVVPARGSLGASGDLAPLAHLALVAIGE
                     GEATLDGAGPGPGGEVLSRAGLSPVALEAKEGLALLNGTQLMAAIGTLMHHDGLRLAL
                     TADVIGALSLEAMLGTAAAFDPRLIEARPHPGQVASARHLRELMADSEIWASHRSDSA
                     HRVQDAYSLRCMPQVHGAARDVLAELRRVLDVEINAATDNPLVFADGAVISGGNFHGE
                     PLALALDYATLAVAELASISERRIARLVDAHLSGGLPPFLTQRPGVESGLMIAHYTAA
                     ALVNELQSLSHPSSVDTIPTSANQEDHVSMGGNAALQLATAVDRCEQVLAIEALCAAQ
                     GIDFRAPLRPGEGLAVAHRRIREVVPHRDADAPPSPDIAAIRDLIHAGDLLANPATAR
                     AHA"
     gene            4670..5422
                     /locus_tag="VFV59_09745"
     CDS             4670..5422
                     /locus_tag="VFV59_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326151.1"
                     /translation="MPDLEFRLVDDEATFEAIPHPMAHGARCHTCDYWERLDGRRDAP
                     AEGATDAEARASLKRSRLRGDTARGGSLGMVGFDGADAVGYVQFGPLSAYPRAQNIRE
                     RYPELPESPAPWVVTCLQVPPATPAVERAGIGAALLTAVCDELDRRGITAVEAYPERA
                     ADPWIPSPGPASVYEAAGFERTAGDDRYPVYRRELSGETDANAWSDLLRASRPADDEE
                     GWPLPLPPKRDEDDFFRLPPEKPKRPNPFGED"
     gene            5427..6167
                     /locus_tag="VFV59_09750"
     CDS             5427..6167
                     /locus_tag="VFV59_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HEU5326152.1"
                     /translation="MLEAGLWGLAAASSLVLGAMAGVWLDVPRRLVALVMGFGAGALI
                     SALAFDLTEEAFNRGGTGWTAVGLALGALTFFIGDTLLERRAKRKAREPAGRGTVAPT
                     SGPAIVLGTLLDGLPESIVLGATLLGGAGVSVSFLAAVFLSNIPEGVAGGRDLSDEGH
                     SRRFIVTLWIGVAVASAIAAGVGNGVLAGMDTAVVGFVQAFAGGAILTMLADTMMPEA
                     FENGGDAVGLTTVLGFAIAFLLSATSAG"
     gene            6200..7882
                     /gene="hutU"
                     /locus_tag="VFV59_09755"
     CDS             6200..7882
                     /gene="hutU"
                     /locus_tag="VFV59_09755"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015593987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU5326153.1"
                     /translation="MTAVRPRSGPRTVRAPRGTELSCRGWQQEAVLRMLMNNLDPEVA
                     EDPDNLVVYGGTGRAARSWEAFDAIVDELRQLGDDETLLVQSGKPVGVLRTTRDSPRV
                     LIANANLVPHWATWEQFRELERAGLTMYGQMTAGSWIYIATQGIIQGTYETFAEAARQ
                     HFGGSLAGTATITAGLGGMGGAQPLAITMNDGAALVVEVDEHRARRRLEAGYVDELTD
                     SSDAAVALVDRWRRDRIGRSVALVANAADVLPELVRQGWKPDLVTDQTSAHDPLGGYV
                     PNGLSLADALALRANDPDDYVRRSTAAMAEHVRAMLAFQRAGAVTFDYGNNIRQVARD
                     AGVDDAFDFPGFVPAFIRPLFCEGKGPFRVAALSGDPHDIAVIDGALAELFPDDAELQ
                     RWLRLAAEKIPVQGLPARICWLGYRDRATAGLRINDLVASGEITAPIVIGRDHLDAGS
                     VASPNRETEAMRDGSDAIADWPILNALVNTAAGATWVSFHHGGGVGIGYSLHAGMVVV
                     ADGTPEAAARLGRVLTTDPAMGVLRHADAGYGRAIEVADERGLRIPMRDRGR"
     gene            7933..8739
                     /locus_tag="VFV59_09760"
     CDS             7933..8739
                     /locus_tag="VFV59_09760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEU5326154.1"
                     /translation="MRRPLLRAAAAVAALLALASPTLGITFGQLDATNQFANVGALIG
                     TYEGNEYLFCTGTLIQDADGGASNIFLTAGHCGDGDGRMSVTFDPTTDMDEATGVFVT
                     GDSVLYEGTFHAHPSFACCGTNDTYDIGVVVLDADVPGVTPAPVASLNLLGTMSKEQL
                     RNATFLTAGYGTVRDDKTRGFASFSFDGDRRFVSQTYQNLLKAWLDLSMQPSTGDGGT
                     CYGDSGGPHFLNGVVVSITSIGDIPCRATDKTYRVDTPVAQDFLAQYLDD"
     gene            8827..9993
                     /locus_tag="VFV59_09765"
     CDS             8827..9993
                     /locus_tag="VFV59_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5326155.1"
                     /translation="MPPPSGGALPSAPDPWAGSEMPPFRSRPPYLMTEMIAAEPALAE
                     RLARRLAGDPAVDALAEAVRTAAAARAPILTTGCGTSEHAAGAVAALLTDALGLTGGR
                     EVTAVQALDGLARPLERGSLLIGVSHEGRTLATNEALTAARAAGATTALITVSGRSPG
                     AEHADLLIETLEQDQSWCHTVGYLSPLIVGVVLAGRLSGRRPDVRALRALLDVTDDHR
                     AASSIASGFTGTDRILVAGWGVDAISARELALKIAEGARLPAAAHDVETLLHGHLAAA
                     NRWTGLVLIRTDQEGEPDVVAARADRLLRAARLLGMPAAAILGERASRAFGADLTPAG
                     RVVLPRGGRVPPVAASLLAPVIPLQLLAERLARARGVNPDTLGREDPAQASAHA"
     gene            complement(9995..11077)
                     /gene="tgt"
                     /locus_tag="VFV59_09770"
     CDS             complement(9995..11077)
                     /gene="tgt"
                     /locus_tag="VFV59_09770"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5326156.1"
                     /translation="MAEPLVTRRGTLPVPTFLPDATRAGVRGVTSDDLRDVGIEGVVV
                     NAFHLLRRPGARVVQAAGGLHRFMDWDGPILSDSGGFQVWSLIRQDPSRGVIRDNEVI
                     FREPETGEKWNLTPERVVNLQFQLGSDIVVCLDDCTDADAPEAEQERSVERTVRWARR
                     CREEFDRQVAQRRSGERPRIFAVVQGGGVEALRRQCATALAEIGFDGYGFGGWPLTGD
                     GALLTDPLRWVAESLPPDVPKHALGIGRADHVVAAFALGYSVFDCALPTRDARHGRLY
                     AFRPGWASRRPSPGDDFFRAVRIHDPGYRVAHEPIEDGCDCQVCTRLSAAYLHHLFKV
                     GDVSAERLATLHNLRFYVRLFDLLRG"
     gene            complement(11070..>11889)
                     /locus_tag="VFV59_09775"
     CDS             complement(11070..>11889)
                     /locus_tag="VFV59_09775"
                     /inference="COORDINATES: protein motif:HMM:NF018758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HEU5326157.1"
                     /translation="VTAEIVERVLRSARYRDVDRNLVERLAGEEVGRSRTSDEAVKRV
                     KRRLHQAVGAFRGRAPSDPLRAAREAWSGDMTSATFREACAHVLRSHASTRERTPFLD
                     GLWPAIWALTGTPRRLLDLGCGLGPVALPWMGLDRRAHYIAVDVDGRPLSVVDAFLDL
                     VAQPHEVREADLAGGLPEVSADVALLLKLVSTLDRQDAAAAARLLNGLDVHHAVVSFP
                     RRSLAGRGKGMEATYRERLDRLVGACGRVSAVAETSVPNELVFVVTLQPRQPRG"
BASE COUNT         1686 a         4424 c         4230 g         1549 t
ORIGIN      
        1 gagcatctcg tccagcagcg gatcggcgga gcggatcccg accatggtgg cgatggcgat
       61 cgggaagaag acgatgagcg cgcagatcac cacccgcgcc agcagcccgc cgccgaacca
      121 gatgtcgagc agcggcgcca gggccaggat cgggacggcc tgcgcggcga cgatgtaggg
      181 cgacaggacc cgctccacca gcacgctctt gccgagcgcg atcccggtga gcacggcggc
      241 ggtgccgccg gcagcgaagc ccagcacgat ctcgagcagc gtcactgccg cgtgctccca
      301 cagcaccccg gtgctgaggg cccgcaccgc tcgctcgacc accgcctcgg gcgccgggag
      361 gatgaactcg gggtatccgc cgatcaccgt caccatcttc caggcgacca ggaaggccgc
      421 caggctgacc agcgccgcac cgatgcgcct catgccgcgc cctccccctc ggcccatggc
      481 cgcagctcca gcgcatgccc ctcggccagc ccggctcgaa cgtcggcggc gagctccgcg
      541 gcgcgtggct ctccaagcag ctgtggcgcg cgaggacgct ccaggtccac ctccacgatg
      601 cgcgcgaccc ggccgggacg cggcgtcatg accaccacgc ggtcggcgag cgcgaccgcc
      661 tcgaccaccg agtgcgtgac cagcaccacg gtccgcggct gctccatcca caggcgctgg
      721 agctcggtgt cgaaggtctc gcgtgtcagc gcatcgagcg cgctgaacgg ctcgtcgagc
      781 aggaggaccg gcggatcggt gatgagcgcc cgcgccagcg cggcgcgctg cgccatcccg
      841 cccgacagct cgcccggccg cagccgtgcg gcaccggcga ggccgacacg ctcgagggcg
      901 agctgcgccc gctcctggcg gtcggacgga gccagcccgt ccagctcgag cggcagcgcc
      961 acgttggcga gggtcgtgcg ccacgggacc agccggggct gctggaaggc gagggcgacc
     1021 cggccgtcgc cggcccgtgg ttcggcgccg ccaacccgca ccgtgccggc cgtgggaccg
     1081 agcagcccgg cgaccaggcg cagcagcgtg ctcttgccgc agccattggg gccgatgatc
     1141 gcggtgaacg agccgggagg gacctcgagc gtcagcccat ccagcgcctg aagcccggag
     1201 tcggcctcgc cgaagcgcac gccgacgccg tcgagtcgaa cgtcgaggcc cgcgctggtg
     1261 gtcctggcac ccatggcttc gctccgatgg tcagccgccg tcggggcatc ggccgacgcg
     1321 acggcacagg gcaccacagc gcccgatgcg cgcacgcgcg gtcgtcttct cccttccgga
     1381 ctgtgaccgt cggccctgtc tgccccggcg agcgccggag gtcaggtcgg ccgcatcggt
     1441 ccttcgaccg atacggttcg cgggctggcc cagtggctgg gccgaccgcc ggtggggact
     1501 tccaccccgc cccgaagacg attggttgct tggtcgattg cgctgatcgt ggcaggtggc
     1561 cgggggccgg tcaacccgcg gcggcccgcc gcttgccgag ccaccagcgg acgacgaagt
     1621 acccaacgat cccgatgacg acgacgtaga tcaggtactc gtacggcttg agcgtggcct
     1681 cgatctgcgg atagttctcg cccacgacga agcccgcgaa gatcagtgcc gcgttccacg
     1741 gcagcgagcc gagcgcgctg aagagcagga agcggcccat gggcatgcgg ctgatcccgg
     1801 cggggtagct gacgaaggtg cgcaccagcg gaaccatgcg gccgaagaac acggcccagc
     1861 tgccccagcg ctcgaaccat cggtccgcac ggtccaggtc atccgcgccg atgccgacgt
     1921 acttcccgta ccgatccagg atcgggcggc cgccccacgc gccgatgtag tagccgacca
     1981 cggcacccac caggctgccg gccgtggcca gcaggatcgc cagcgggaag ttccacggca
     2041 gccccgtgag cggctcgagg acgttgggat ccgtggccga ctggctgatc ttccagcccg
     2101 ccatcggcag gatgacctcc gacgggatcg gcgccacgat ggtctccagc gccacggcca
     2161 gggcgacgcc gacgtagccg acggcgtcgt acatctggag gatgaacggg atgacctccc
     2221 ggtcgatgaa atcgagcagc ggtcagcctc cgaccggctc gccggacgag gtgttcgacg
     2281 ccggggctcc cgcgtccggt gccgcgggcg cggtccggcg cggcacggcg acccgtcgct
     2341 ggccggcgcc tgaacgcatg gctgcctccc gcaggcgggc gcgctcatcg acgcggagga
     2401 cgtgcaccgc caccaggatc gcgaccagcg cgccaccggc catccagccg cccagcacat
     2461 ccatcgggta gtgggcgccc aggtagatcc gggtggcgct gatggcgccg atgagcacca
     2521 gggcgacggc gtaggcgatc tcgccctgtc gacgatcgtg ggccaggcgg gcaacgcaga
     2581 acgcggcgag gccgtagagg gccgtcgccc gcagcgcgtg gccggccggg aagtcggcga
     2641 tgtcatccag gccgatgttg ttgacgttga acatcgcgct cacgaggtcg ccgatgctga
     2701 tcgcctcggc cgaggtgaag tacttcagcg cccactccat ggggaagcag agggtcccga
     2761 gggccagcag gaaggcgagg tcccggcggt tcttggcgag cgccagcaga ccgagggcgg
     2821 cgaaggcgag ggccacgaac cagccggagc cgagggcgct gatggcgatg atcagcacct
     2881 cgacgggcgg gaggtttgcc gccacccagc tgtggaaggc aggcaccacg ttcgacatga
     2941 accactcctc cggcgccgtg tagaccgtgg tgcgcttgag catcacgagc cagaagaggg
     3001 ccggcagggc gattaccgag aggaggaagg cgacggcggg gctggggacc gcgccaccca
     3061 ggcgcagggc cgcgtcgtcc tcgacgacga ggcggctggt ggggtccggc agccgtcgca
     3121 cggtgcgtag tatacggacc gatgccgccg ctcctgctca ccggccggga cctcaccatc
     3181 gacaacgtgg tcgaggtggc gcgcgggcgg cgccgcgtgg agctgacccc ggaggccgcc
     3241 gaccgcatgc gggccagccg cgcggtcatc gagcggctgg tggccgatgg cggcaccgtc
     3301 tacggcgtga cgaccggctt cggcgacctc gccgacgtgc gcatcgagcc cgaccagacg
     3361 gccgacctgc agcacaacct cgtccgcagc cacgccgccg gcgtcggtga gccactcccc
     3421 gacgacgtcg tgcgggccat gctcctgctg cgtgccaatg cgctggccgt cggcctgtcc
     3481 ggcgttcgcg tcgaggtcgt cgagctgctg ctcggcatgc tcaacgccgc cgtgcacccc
     3541 gtggtgccgg cgcgcggcag cctcggggcg tcgggcgacc tcgccccgct cgcccacctc
     3601 gcgctggtcg ccatcggcga gggcgaggcg acgctcgatg gcgcggggcc cggtcccgga
     3661 ggcgaggtgc tgtcgcgcgc cggcctgtca cccgtggcgc tggaggccaa ggagggcctg
     3721 gccctcctca acgggacgca gctgatggcc gccatcggca cattgatgca ccacgacggc
     3781 ctgcgcctgg cgctgaccgc cgacgtgatc ggggcactga gcctggaggc gatgctcggc
     3841 acggccgccg cgttcgaccc gcggctcatc gaggcgcgcc cacatccagg gcaggtggcc
     3901 agcgcccgcc acctgcggga gctcatggcg gacagcgaga tctgggcgtc gcatcggtcc
     3961 gattcggccc atcgcgtcca ggacgcctac agcctgcggt gcatgccgca ggtccacggc
     4021 gcggcgcgcg acgtgctcgc cgagctccgg cgcgtgctgg acgtcgagat caacgccgcg
     4081 accgacaacc cgctcgtctt cgccgacggt gccgtcatct cgggcggcaa cttccacggc
     4141 gagccgctgg ccctggcgct cgactacgcg acgctggcgg tggcggagct cgcctcgatc
     4201 tcggagcggc ggatcgcccg gctcgtcgac gcgcacctgt ccggcgggct gccgccgttc
     4261 ctgacccagc ggccgggcgt cgagagcggg ctgatgatcg cccactacac ggccgccgcg
     4321 ctggtcaacg agctccagtc cctgagccac ccctcgtcgg tcgacaccat cccgaccagc
     4381 gccaaccagg aggaccacgt cagcatgggt ggcaacgccg cactgcagct ggccaccgcg
     4441 gtggaccgat gcgagcaggt gctcgccatc gaggcgctgt gcgccgccca gggcatcgac
     4501 ttccgcgcgc ccctgcgtcc cggcgagggg ctggcggtgg cgcaccggcg catccgcgag
     4561 gtcgtgccgc atcgcgacgc cgatgccccg ccatcgccgg acattgccgc gatccgcgac
     4621 ctgatccacg ccggggatct gctggccaat cccgcgaccg cacgtgccca tgcctgacct
     4681 ggagttccgc ctggtggacg acgaggcgac cttcgaggcc atccctcacc cgatggcgca
     4741 cggcgcccga tgccacacct gcgactactg ggagcggctc gacggccgcc gcgacgcgcc
     4801 ggccgaggga gcgacggatg cggaggctcg cgcatccctc aagcgatcac gactgcgcgg
     4861 cgatacggcg cgcgggggca gcctcggcat ggtgggcttc gacggggccg acgccgtggg
     4921 ctatgtacag ttcggacccc tctcggccta cccccgggcg cagaacatcc gcgagcgcta
     4981 cccggagctc ccggagtcgc ccgcgccgtg ggtggtcacc tgcctgcagg tcccgcccgc
     5041 cacgccggcc gtcgaacgtg ccggcatcgg cgccgccctg ctgacggccg tctgcgacga
     5101 gctggatcgg cgtggcatca ccgcggtcga ggcgtacccc gagcgcgccg cggacccgtg
     5161 gataccgtcg ccgggtccgg catcagtcta cgaggcggcg gggttcgagc gcaccgcggg
     5221 cgacgatcgg taccccgtct accggcggga gctgtcgggc gagaccgatg cgaacgcctg
     5281 gtcggacctg ctgcgcgcgt cgcgccccgc ggacgacgag gagggctggc cgctcccgct
     5341 gccgccgaag cgcgacgagg acgacttctt ccgcctgccg cccgagaagc cgaagcgccc
     5401 gaacccgttc ggcgaggact gagggcgtgc tcgaagcggg tctctggggc ctggccgccg
     5461 cttcgtcgct ggtgctcggt gcgatggcgg gggtctggct cgacgttccc cggcgcctcg
     5521 tcgccctggt catgggattc ggggccggcg cgctcatcag cgccctcgcc ttcgacctga
     5581 ccgaggaggc cttcaaccgg ggcgggacgg gctggacggc ggtcggcctg gcgctcgggg
     5641 cgctgacctt cttcatcggc gacaccctgc tcgaacgccg cgccaagcgg aaggcgcgcg
     5701 agccggccgg ccggggcacc gtcgcgccga ccagcgggcc cgccatcgtc ctcggcacgc
     5761 tgctggacgg gctgcccgag tcgatcgtgc tcggggcgac gctgcttggc ggcgccggcg
     5821 tctcggtctc cttcctggcc gccgtcttcc tctcgaacat ccccgagggc gttgccggcg
     5881 gccgcgacct gtcggacgag gggcactcgc gccggttcat cgtcacgctg tggatcggcg
     5941 tggccgtcgc ctccgccatc gcggcgggcg tgggcaacgg ggtgctggcg ggcatggaca
     6001 ccgcggtcgt cggcttcgtg caggccttcg ccggcggcgc gatcctgacc atgctggccg
     6061 acacgatgat gcccgaggcg ttcgagaacg gcggggatgc ggtcgggctc accaccgtcc
     6121 tcgggttcgc catcgccttc ctgctgtcgg ccaccagcgc cggatgagcg cccgatgagg
     6181 gccggcgtat gatcggcgca tgacggccgt ccgtccgcgc agcggccccc gaaccgtccg
     6241 tgctccgcgc ggcaccgagc tctcctgccg cggctggcag caggaggccg tgctgcggat
     6301 gctcatgaac aacctcgacc ccgaggttgc cgaggacccg gacaacctcg tcgtgtacgg
     6361 cggcaccggt cgcgcggcac ggtcctggga ggcgttcgac gccatcgtcg acgagctgcg
     6421 ccagctgggc gacgacgaga cgctgctggt ccagtccggc aagccggtcg gggtgctacg
     6481 cacgacccgc gatagcccgc gcgtgctgat cgccaacgcc aacctggtgc cgcattgggc
     6541 cacctgggag cagttccgcg agctggagcg cgccggactg accatgtacg gccagatgac
     6601 cgccggctcg tggatctaca tcgcgaccca ggggatcatc cagggcacct acgagacctt
     6661 cgccgaagcc gcgcgccagc acttcggcgg ctccctggcc gggacggcca ccatcaccgc
     6721 cgggctgggc ggcatgggcg gggcccaacc gctggccatc accatgaacg acggcgccgc
     6781 cctggtcgtc gaagtggacg agcaccgggc gcggcgccgc ctggaggcgg ggtacgtcga
     6841 cgagctgacg gactcctcgg acgcggcggt cgccctggtc gaccgctggc gccgcgaccg
     6901 catcggccgt tcggtggcgc tcgtcgccaa cgccgccgac gtgctgccgg agctggttcg
     6961 gcaaggctgg aagccggacc tcgtcaccga ccagacctcc gcccatgacc cgctgggcgg
     7021 gtacgtcccc aacgggctct ccctcgccga tgcgctggcg ctgcgcgcca acgatcccga
     7081 cgactacgtc cgccgctcga ccgccgcgat ggcggagcac gtgcgcgcca tgctcgcctt
     7141 ccagcgcgcg ggggcggtga ccttcgacta cggcaacaac atccgccagg tcgcgcgcga
     7201 cgccggcgtc gacgacgcct tcgacttccc cggcttcgtg ccagccttca tccgcccgct
     7261 cttctgcgag ggcaagggtc cgttccgggt ggcggcgctg tccggcgatc cgcacgacat
     7321 cgcggtcatc gacggcgcgt tggccgagct gttccccgac gacgcggagt tgcagcgctg
     7381 gctgcgcctg gcggcggaga agatcccggt ccagggcctg ccggcgcgca tctgctggct
     7441 gggctaccgc gaccgcgcca cggccggcct gcgcatcaac gacctggtcg cgtcgggcga
     7501 gatcaccgcc ccgatcgtca tcggccgcga ccacctcgat gcaggcagcg tcgccagtcc
     7561 caaccgcgag accgaggcca tgcgcgacgg ctccgacgcc atcgctgact ggccgatcct
     7621 caacgccctc gtcaacaccg ccgccggtgc cacctgggtc agtttccacc acggtggggg
     7681 cgtcggcatc ggctactcgc tccacgccgg gatggtggtc gtcgccgacg ggacgcccga
     7741 ggccgcggca cgcctggggc gcgtgctgac cactgacccc gcgatgggcg tgctgcgcca
     7801 cgccgatgcc ggctacgggc gcgccatcga ggtcgcggac gagcgcggcc ttcgcatccc
     7861 gatgcgcgat cgaggccgtt gacgccccgg cgcatcatgg gtccccgccc aatcgaagac
     7921 tgagggaccc gcatgcgacg accacttctt cgcgccgcgg ctgccgtcgc ggcactgctc
     7981 gccctggcca gcccgaccct gggcatcacc ttcggccagc tcgacgcgac gaaccagttc
     8041 gccaatgtcg gcgcactcat cgggacgtac gaaggcaacg agtacctctt ctgcaccggc
     8101 accctcatcc aggacgcgga cggcggggcg tcgaacatct tcctcaccgc ggggcactgc
     8161 ggggacggcg acgggcggat gtccgtcacc ttcgatccga cgaccgacat ggatgaggcg
     8221 accggggtct tcgtgaccgg tgactccgtg ctgtacgagg ggacgttcca cgcccacccg
     8281 tcgttcgcct gctgcggcac gaacgacacg tacgacatcg gcgtcgtggt gctggacgcg
     8341 gacgtcccag gcgtcacccc ggccccggtc gcgagcctca acctcctcgg cacgatgtcg
     8401 aaggagcagc tgcgcaacgc gacgttcctc accgccggct acggcaccgt gcgcgacgac
     8461 aagacgcgcg gattcgcctc gttcagcttc gacggcgacc gtcggttcgt cagccagacc
     8521 taccagaacc tgttgaaggc gtggctcgac ctctcgatgc agccctcgac cggcgacggc
     8581 ggcacctgct acggcgacag cggcggcccg cacttcctga acggtgtcgt ggtctccatc
     8641 acgtcgatcg gcgacatccc gtgccgcgcc accgacaaga cctatcgcgt cgacacgccg
     8701 gtggcgcagg acttcctggc gcagtacctg gacgactgac cttcctgcag ctggaccgat
     8761 ccggccgccc ggacgcatgc gtccgcggcg gccgctcctc gtcggcggac gcggcatgat
     8821 gtgaccatgc ctccaccctc cggcggcgcg ctcccctcag ctcccgaccc atgggccggg
     8881 tccgagatgc cgcccttccg gtcgcgaccg ccgtacctca tgacggagat gatcgcggcc
     8941 gagccggccc tggccgaacg cctggcccga cggctcgcgg gcgaccccgc ggtggacgcc
     9001 ctggccgagg ccgttcggac ggccgccgcg gctcgcgcgc ccatcctgac gaccggctgc
     9061 ggaacgtccg agcacgccgc cggggcggtc gccgcgctcc tgaccgatgc cctggggctg
     9121 acgggcggcc gcgaagtgac ggcggtccag gcgctggacg gcctcgcccg gccgctggag
     9181 cgcggcagcc tgctgatcgg cgtgtcgcac gagggccgga ccctcgcgac gaacgaggcc
     9241 ctcaccgccg cgcgtgctgc gggggcgacg acggcgctga tcacggtcag tggccgctcg
     9301 ccgggcgccg agcacgcgga tctgctgatc gagacgctcg agcaggacca gagctggtgc
     9361 cacacggtcg gctacctgtc gccgctgatc gtcggggtcg tgctggccgg gcgcctctcc
     9421 ggccgccgtc ccgacgtccg ggcgttgcgc gcgctgctgg acgtgaccga cgaccaccgc
     9481 gccgcctcca gcatcgcgtc gggctttacc ggcaccgatc gcatcctcgt cgccggctgg
     9541 ggcgtcgacg ccatctccgc ccgcgagctt gccctgaaga tcgccgaggg ggcgcgcctc
     9601 cccgccgccg cccacgacgt cgagaccctg ctccacggcc acctggcggc ggcgaatcgc
     9661 tggaccggcc tcgtcctcat ccgaacggac caggagggtg aaccggacgt ggttgcggcc
     9721 cgtgccgacc gactcctgcg ggccgcgcgg ctcctgggga tgccggcggc cgccatcctg
     9781 ggggagaggg cgtcgcgcgc gttcggtgcc gacctcacgc cggcggggcg ggtggtgctg
     9841 ccgcgcggcg gacgcgtccc cccggtcgcc gcctcattgc tggcccccgt catcccgctg
     9901 caattgctgg ccgagcggct ggcccgcgcc cggggcgtga accccgacac cctcgggcgc
     9961 gaggatccgg cgcaggcatc ggcccacgcc tgagctaccc gcgcagcagg tcgaacagcc
    10021 ggacgtagaa gcgcaggttg tggagcgtgg cgagccgctc ggcggacacg tcgccgacct
    10081 tgaacaggtg gtgcaggtag gccgccgaca ggcgcgtgca gacctggcaa tcgcagccgt
    10141 cctcgatcgg ctcgtgcgcg acgcggtaac ccggatcgtg gatgcggacc gcgcggaaga
    10201 agtcgtcgcc gggtgacggt cgtcgggacg cccacccggg gcggaaggcg tacaaccggc
    10261 cgtggcgagc atcccgcgtg ggcagggcgc agtcgaacac cgagtacccc agcgcgaacg
    10321 cggccaccac gtggtccgcg cgcccgatcc cgagcgcgtg cttcggcaca tcgggcggca
    10381 gcgattcggc gacccagcgc agcggatcgg tgagcagggc gccgtcgccg gtgagcggcc
    10441 agccgccgaa gccgtagccg tcgaagccga tctcggcgag cgctgtcgca cactgccgcc
    10501 gcagcgcttc caccccgccg ccctgcacca ccgcgaagat ccgcggccgc tcaccggacc
    10561 ggcgctgcgc cacctgtcgg tcgaactcct ctcgacagcg acgggcccac cggacagtcc
    10621 gctcaacgga ccgctcctgc tcggcttcgg gggcgtccgc atcggtgcag tcgtccaggc
    10681 agaccacgat gtcggagccg agctggaact gcaggttgac cacccgctcc ggggtcaggt
    10741 tccacttctc gccggtctcg ggctcccgga agatgacctc gttgtcgcgg atgacgcccc
    10801 ggctggggtc ctggcggatc aggctccaca cctggaaccc gccggagtcg ctgaggattg
    10861 gcccatccca gtccatgaac cgatgcaggc cgcccgcggc ctggacgacg cgcgcccccg
    10921 ggcggcgcag caggtggaag gcgttgacga cgacgccctc gatgcccacg tcgcgcaggt
    10981 cgtcggaggt cacgccgcgc acgccagcgc gggtcgcgtc gggcaggaac gtcggcacgg
    11041 gcagcgtgcc gcgtcgcgtg acgagcggct cagccacgcg gctgcctcgg ctggagggtc
    11101 accacgaaga cgagctcgtt cgggaccgat gtctccgcga ccgcgctgac gcgaccgcac
    11161 gccccgacga gccggtcgag gcgttcacgg tatgtcgcct ccattccctt gccgcgacca
    11221 gccagcgaac ggcgcgggaa cgacaccacg gcgtggtgca cgtccagccc gttgagcagc
    11281 cgagcagcgg ctgcagcgtc ctggcggtcg agggtcgaga ccagcttgag gagcagcgca
    11341 acgtccgccg atacctccgg caggccacca gcgaggtccg cctcccgcac ctcgtgtggc
    11401 tgcgcgacga ggtcgaggaa ggcgtccacg acggacagcg gtcgcccgtc gacgtcgacc
    11461 gcgatgtagt gcgcccgtcg gtcgagcccc atccatggca acgccactgg accgagcccg
    11521 caccccaggt cgagcagtcg ccgcggcgtc ccggtcagcg cccagatggc cggccagagg
    11581 ccgtcgagga acggcgtccg ctcgcgggtc gaggcgtggc tgcgcaggac gtgggcgcag
    11641 gcctcacgaa acgtcgcgct cgtcatgtca ccggaccagg cctcccgcgc ggcgcggagc
    11701 ggatcggacg gcgcgcggcc acggaacgcg ccgaccgcct ggtgcaggcg tcgcttcacg
    11761 cgcttcaccg cctcgtcgct cgtgcgcgac cggcccacct cctcgccggc cagccgctcc
    11821 accaggttgc ggtccacgtc gcggtaacgg gcggagcgca gcacgcgctc gacgatctcg
    11881 gccgtcact
//
LOCUS       DASTYP010000224         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_932255, whole genome shotgun sequence.
ACCESSION   DASTYP010000224 DASTYP010000000
VERSION     DASTYP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932255"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFV59_09780"
     CDS             <1..687
                     /locus_tag="VFV59_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5326158.1"
                     /translation="ILPAWSDADADLHARLGDAIDPDRKVLAALERIMPLSGKRIADV
                     GTGIGHYPMLLARRTGRTYGVESDPELLAEARRRAAGSHQPNIRIVEGASTSLPLRDG
                     AVDLVLTSLIEPDDASLPAVREALRVLRPGGRLVVIGYYGRDDVGALLEPEVVAHVRD
                     ATQRRTGWWLRNGFKIKVVHARLDLGDADTAVELLPRLYGDRGRAFLMGPHRSSLRLN
                     LGLYHRGTDA"
     gene            954..2543
                     /locus_tag="VFV59_09785"
     CDS             954..2543
                     /locus_tag="VFV59_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5326159.1"
                     /translation="MASTTPRMKITYATLSADNEELQSAFDAALERARSSMGETHPMI
                     IGEEQRHAETTFEDRSPIDPDLVVARFPTGTRQDARDAIDAARAAYPGWRDTPWRDRL
                     AIMRRAADLISERQFDYAALMAIEVGKNRLEALGDVEETADLLRYYSDEFEKADGFVK
                     PMGSLSPMEHTRSVLRPYGVFAVISPFNFPMALSGGPSAGAMIAGNTVVLKPSSDAPL
                     LAWKFGEALRDAGLPPGVFNLVTGPGETVGAELEENPGVDGMVFTGSYEVGMRLHKGF
                     TRDFPRPIITEMGGKNPAIVTRHADLDLAAEGAMRSAFGYDGQKCSALSRVYVEKEVA
                     RDFVERLVERTKAIKVGDPTRRENWMGPVINQRALDRFRNAVDEAKADGGTIEIGGEV
                     LANERGYFPTPTVVTGLPLEHRLFRDELFVPFLVVGEVDSLDQALAEANGTAYGLTAG
                     IFSEDEAEVDRFLRAIEAGVVYVNRRAGATTGAWPGIQSFGGWKGSGSSGKGGLGPYY
                     VQQFLREQSQTIIGDEEPARL"
     gene            2540..2938
                     /locus_tag="VFV59_09790"
     CDS             2540..2938
                     /locus_tag="VFV59_09790"
                     /inference="COORDINATES: protein motif:HMM:NF025484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HEU5326160.1"
                     /translation="MNGDEDVVVEPSGWFAVQLRYAYLTGDDDAVSLVEDRVILVDGG
                     DEEAALGRAVELAPEYEDDYETEDGERGTIRFEGIVAIKELDDPPAAGTEVWHEFMSP
                     DPSRSAVDEDSEPLPPVYPEEMAFPRAHDD"
     gene            2949..>3379
                     /locus_tag="VFV59_09795"
     CDS             2949..>3379
                     /locus_tag="VFV59_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEU5326161.1"
                     /translation="MSEWRIAAYHAVRPLLFRVDPERIHALALRALALAGGSSPGRAL
                     CRFVAGVGDPADAPVELAGLRFRNRIGLGAGFDKDGVAIRGWAALGFGFVELGTVTPH
                     PQPGNPRPRLFRLPPDEALVNRMGFNNAGADALARRIAEARP"
BASE COUNT          504 a         1199 c         1214 g          462 t
ORIGIN      
        1 atccttccgg cctggtccga tgccgacgcc gatctccacg cccggctggg cgacgcgatc
       61 gaccccgatc gcaaggtgct cgccgcgctg gagcggatca tgccgctcag cgggaagcgc
      121 atcgccgacg tcgggaccgg catcgggcac tacccgatgc tgctggcgcg gcgcacgggc
      181 cgtacgtacg gcgtcgagtc ggatccggag ctgctggccg aagcgcgccg gcgagcggcc
      241 ggcagccacc agccgaacat ccggatcgtg gagggagcgt cgacgtcgct gccgctgcgc
      301 gacggggcgg tggacctggt gctcacctcg ctgatcgagc cggacgacgc ctcgctgccg
      361 gcggtccgcg aggccctgcg cgtgcttcgt ccgggcggcc ggctggtggt catcggctac
      421 tacggccggg acgacgtcgg cgccctgctg gagccggagg tcgtggcgca cgttcgcgac
      481 gcgacccagc gacggacggg ctggtggctg cgcaacgggt tcaagatcaa ggtcgtgcac
      541 gccaggctgg acctgggcga cgcggacacg gcggtcgagc tcttgccccg cctctacggg
      601 gaccgcggac gggcgttcct gatggggccg caccgctcgt ccctccggct caacctgggc
      661 ctgtaccacc gcggcacgga cgcctgaccc agtcccgatt ccggagaaaa cagagatctg
      721 cgtgcgaaat ccgtggattc catgcgggtt gcggccacgg atcagtctgg atatgatgcc
      781 gctgctgagg gccaggctct tctccccccg gagcctggcc ctcggcatgc ccggcgcgcg
      841 gtccaacgga cccccggtgc gataatggac gccttccgag actcgccgat cgggccgccg
      901 cgaccgcggc ccgcacgacc gaacacgcac aggacgcagg aggcggacgg acgatggcca
      961 gcaccacgcc gcggatgaag atcacctacg ccacgctctc ggcggacaac gaggagctcc
     1021 agtcggcctt cgacgcggcg ctggagcgcg cccggtcgtc gatgggcgag acccatccga
     1081 tgatcatcgg cgaggagcag cgccacgccg agacgacctt cgaggaccgg tcgcccatcg
     1141 atccggacct cgtcgtcgcc cgcttcccga ccggcacgcg acaggacgcc cgcgacgcca
     1201 tcgacgcggc gcgcgccgcg taccccggct ggagagacac cccatggcgc gatcggctgg
     1261 ccatcatgcg gcgcgccgcc gacctcatca gcgagcgcca gttcgactac gccgccctca
     1321 tggccatcga ggtcggcaag aaccgcctgg aggcgctcgg agacgtcgag gagaccgcgg
     1381 acctgctgcg ctactacagc gacgagttcg agaaggccga cggcttcgtg aagccgatgg
     1441 gctcgctctc gccgatggag cacacgcgct cagtcctgcg gccgtatggc gtcttcgccg
     1501 tcatcagccc cttcaacttc ccgatggcgc tctcgggcgg tccctcggcc ggcgccatga
     1561 tcgcgggcaa caccgtggtc ctcaagccga gctccgacgc gccgctgctg gcctggaagt
     1621 tcggcgaggc gctgcgcgac gccggcctgc cgcccggcgt cttcaacctg gtcaccggtc
     1681 ccggcgagac ggtcggggcc gagctggagg agaaccccgg cgtcgacgga atggtcttca
     1741 ccggctcgta cgaggtcggg atgcggctgc acaagggctt cacgcgcgac ttcccgcggc
     1801 cgatcatcac cgagatgggc gggaagaacc cggccatcgt caccaggcac gcggacctcg
     1861 acctcgccgc ggagggcgcg atgcgctcgg cgttcggcta cgacggccag aagtgctcgg
     1921 cgctgtcgcg cgtgtacgtc gagaaggagg tcgcgcggga cttcgtggag cggctcgtcg
     1981 agcgcacgaa ggccatcaag gtgggcgacc ccacccgacg cgagaactgg atgggccccg
     2041 tcatcaacca gcgggccctg gaccggttcc gcaacgcggt cgacgaggcg aaggccgatg
     2101 gcggcaccat cgagatcggc ggggaggtcc tcgccaacga gcgcggctac ttccccacgc
     2161 caaccgtcgt caccgggctg ccgctggagc atcggctctt ccgcgatgag ctgttcgtgc
     2221 cattcctggt ggtcggcgag gtcgactcgc tggaccaggc gctggcggag gccaacggca
     2281 cggcgtacgg gctgacggcc ggcatcttca gcgaggacga ggccgaggtc gatcggttcc
     2341 tgcgcgccat cgaggccggt gtcgtgtacg tcaaccggcg cgccggcgcg acgaccgggg
     2401 cgtggcccgg gatccagagc ttcggcgggt ggaagggatc cggctcgtcc ggcaagggcg
     2461 gcctgggccc ctactacgtg cagcagttcc tgcgcgagca gtcgcagacg atcatcggcg
     2521 acgaagagcc ggcacggctg tgaacggcga cgaagacgtc gtcgtcgagc cctcgggttg
     2581 gttcgccgtg cagctccgct acgcgtacct gacgggggac gacgacgcgg tctcgctggt
     2641 ggaggaccgc gtgatcctgg tggacggcgg cgacgaggag gccgcgctgg gacgcgccgt
     2701 cgagctggcg cccgagtacg aggacgacta cgagaccgag gacggggagc gcggcaccat
     2761 ccggttcgag gggatcgtgg cgatcaagga gctcgacgac ccgcccgccg ccgggaccga
     2821 ggtgtggcac gagttcatgt cacccgatcc gtcgcgctcg gccgtcgacg aggacagcga
     2881 gcctctgcct ccggtgtatc cggaggagat ggcgttcccc cgcgcccacg acgactgacc
     2941 ccggaccgat gagcgagtgg cggatcgcgg cgtaccacgc cgtccgtcca ctcctcttcc
     3001 gcgtcgatcc ggagcggatc cacgccctgg ccctgcgcgc gctggcactc gccggcggct
     3061 cgtcgcccgg acgagcgctg tgccgcttcg tcgccggcgt gggcgacccg gccgacgcgc
     3121 ccgtcgagct cgccgggctg cgattccgca accgcatcgg cctgggtgcc ggcttcgaca
     3181 aggatggcgt cgccatccgg ggatgggcgg ccctgggctt cggcttcgtg gagctcggga
     3241 cggtgacccc gcatccgcag ccgggcaatc cccgcccccg cctcttccgg cttccgcccg
     3301 acgaggccct ggtcaaccgc atgggcttca acaacgccgg agccgatgcg ctggcgcgcc
     3361 ggatcgccga ggcccggcc
//
LOCUS       DASTYP010000225         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_714303, whole genome shotgun sequence.
ACCESSION   DASTYP010000225 DASTYP010000000
VERSION     DASTYP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714303"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFV59_09800"
     CDS             <1..373
                     /locus_tag="VFV59_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU5326162.1"
                     /translation="MVFFEAPHRLEAALTAMADAFGDDRAAAVCRELTKTYEEVRRGG
                     LRELTTWAAAGVKGEVTVVVAGAEPATTPAAADLVAEVRALVDSGQRLKEATAEVAAA
                     HGASRRELYEAVLADRPASTS"
     gene            406..1581
                     /locus_tag="VFV59_09805"
     CDS             406..1581
                     /locus_tag="VFV59_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5326163.1"
                     /translation="MEFRRIPGLPPYVFTIIDGLKREARRAGRDVVDLGFGNPDLPSP
                     TIAVEKLAEAARNSRNHRYSASRGIPKLREAVAALYQRKFGVTLDPETEVLSTIGAKE
                     GFSHLMWVLLQPGDAALVPSPSYPIHIWGPYFAGADARQVPVGTGEDFVANVMEAWDL
                     GWPKPRVVVLSFPHNPTTATIELADLQRLVDWARDREVVLVHDFAYADVAFDGLKPPS
                     ILQCEGARECAVELYSMTKSFSMAGWRVAFLVGNSEVVAALTKLKSYLDYGTFQPIQI
                     AATVTMNEASDYPAEVNAVYESRRNALVDGLSRIGWEIERPRGTMFAWAPIPEPYRDL
                     GSIEFATLMVQDCDVAVSPGVGFGPGGDGHVRFALIENEQRIVQAVRQLKRGLPKLG"
     gene            1616..>2751
                     /gene="metG"
                     /locus_tag="VFV59_09810"
     CDS             1616..>2751
                     /gene="metG"
                     /locus_tag="VFV59_09810"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5326164.1"
                     /translation="MSRNVLTAVAWPYANGPRHIGHVSGFGVPSDVFSRYMRMAGHNV
                     LMVSGTDEHGTPIQVQADAEGMKTRELADRYNRIIVEDLARLGLSYDLFTRTTTVNHY
                     DVVQEMFATLHRNGYIVAKTTMGAISPSTGRTLPDRYIEGTCPICGYDGARGDQCDNC
                     GNQLDPIDLINPHSRINGETPDFVETEHFFLDLPALADALGAWLDERTDWRPNVLKFS
                     RNLLKDLQPRAITRDLDWGVPIPLDDWEARADKRIYVWFDAVIGYLSASIEWARRSGD
                     PEAWRRWWRDPDASSYYFMGKDNIVFHSVIWPAILLGYEGTGDKDGEPGALGALQLPT
                     ELVSSEFLTMEGRKFSSSRSVVIYVRDFLDRYDADALRYFIAVAG"
BASE COUNT          425 a          973 c          949 g          404 t
ORIGIN      
        1 gatggtcttc ttcgaggcgc cgcaccgcct ggaggcggcc ctgaccgcca tggccgacgc
       61 gttcggagac gatcgggcgg ccgccgtgtg ccgcgagctg accaagacct acgaggaggt
      121 acgccgcggc gggctgcgcg agctgacgac gtgggccgcg gctggggtca agggcgaggt
      181 cacggtcgtc gtcgcgggtg ccgagccggc gaccacgccg gccgcagctg acctggtggc
      241 cgaggtccgc gccctggtgg actcgggaca gcggctcaag gaggcgacgg ctgaggtcgc
      301 ggccgcccac ggggccagcc ggcgggagct ctacgaggcg gtgctggccg acaggccggc
      361 gtccacgtcc tgagacgggg cgccggacgt cactagcctg ggtgcatgga gttccgccgc
      421 atccctggcc tgccgccgta cgtcttcacc atcatcgacg gactcaagcg tgaggcgcgg
      481 cgggccggac gggacgttgt cgacctcggc ttcggcaacc cggacctccc gtcgccgacg
      541 atcgcggtgg agaagctcgc ggaggcggcg cggaacagcc gaaaccaccg ctactccgcc
      601 agccgcggca tccccaagct gcgcgaggcg gtcgccgccc tctaccagcg caagttcggg
      661 gtgaccctgg accccgagac cgaggtgctg tcgaccatcg gcgccaagga gggtttcagc
      721 cacctcatgt gggtgctgct ccagcccggc gacgcggcgc tcgtgccgtc accgtcctac
      781 ccgatccaca tctggggccc gtacttcgcc ggcgcggacg cccggcaggt gccggtcggg
      841 accggtgagg acttcgtcgc caacgtcatg gaggcgtggg acctgggctg gccgaagccg
      901 cgggtggtgg tgctgtcctt cccgcacaac ccgaccaccg cgaccatcga gctggccgac
      961 ctgcagcggc tggtggactg ggcccgtgat cgagaggtcg tgctggtgca cgacttcgcg
     1021 tacgccgacg tcgccttcga cggcttgaag ccaccgtcga tcctgcagtg cgaaggcgcg
     1081 agggagtgcg ccgtcgagct ctactcgatg acgaagtcgt tctcgatggc cggctggcgg
     1141 gtcgccttcc tcgtcggcaa cagcgaggtg gtcgccgcgc tgaccaagct caagagctac
     1201 ctcgactacg gcacgttcca gccgatccag atcgccgcga cggtcacgat gaacgaggcc
     1261 tccgactacc cggccgaggt caacgcggtc tacgagtcgc gccgcaacgc cctcgtggac
     1321 gggctgtccc ggatcggctg ggagatcgag cggccccgcg ggacgatgtt cgcctgggcg
     1381 ccgatccccg agccgtaccg cgacctcggc tcgatcgagt tcgcgacgct gatggtgcag
     1441 gactgtgacg tggccgtgtc gccgggcgtc ggtttcgggc cagggggcga cgggcacgtc
     1501 cggttcgcgc tgatcgagaa cgagcagcgg atcgtccaag ccgtccgcca gctcaagcgc
     1561 gggctgccca agctcggctg acaaaccggt tgggcggcgc ccgtaggctg gacgcatgtc
     1621 ccgcaacgtc ctcaccgctg tggcctggcc ctatgccaac ggcccgcggc acatcggtca
     1681 tgtctccggt ttcggggtcc cctccgacgt gttcagccgg tacatgcgga tggcggggca
     1741 caacgtcctc atggtcagcg ggaccgacga gcacggcacc ccgatccagg tgcaggccga
     1801 cgcggaaggc atgaagaccc gcgagctcgc cgaccggtac aaccggatca tcgtcgagga
     1861 cctcgcgcgg ctcggcctgt cctacgacct gttcacgcga acgaccacgg tcaaccacta
     1921 cgacgtcgtc caggagatgt tcgccaccct gcaccggaac ggctacatcg tcgcgaagac
     1981 cacgatgggc gcgatctcgc cgtccaccgg gcgcacgctg ccggaccgct acatcgaggg
     2041 cacctgcccg atctgcgggt acgacggggc gcgcggggac cagtgcgaca actgcgggaa
     2101 ccagctcgac ccgatcgacc tgatcaaccc gcacagccgg atcaacggag agacgccgga
     2161 cttcgtcgag accgagcact tcttccttga cctgcccgcg ctcgccgacg ccctgggcgc
     2221 ctggctcgac gagcgcaccg actggcgacc caacgtcctg aagttctccc ggaacctcct
     2281 caaggacctc cagccccggg ccatcacccg cgacctcgac tggggggtgc cgatcccgct
     2341 cgacgactgg gaggcccgcg ccgacaagcg catctacgtc tggttcgacg ccgtcatcgg
     2401 ctacctctcg gcgtcgatcg agtgggcccg gcgcagcggc gaccccgagg cctggcgccg
     2461 ctggtggcgg gacccggacg cctcgtcgta ctacttcatg ggcaaggaca acatcgtctt
     2521 ccactcggtg atctggccgg cgatcctcct cgggtacgag ggcacgggag acaaggacgg
     2581 cgagcccggt gcgctgggcg cactgcagct gccgaccgag ctcgtctcca gcgagttcct
     2641 cacgatggag ggccgcaagt tctcgtcctc ccggtcggtc gtcatctacg tccgcgactt
     2701 cctggaccgc tacgacgccg acgcgctgcg ctacttcatc gcggtggccg g
//
LOCUS       DASTYP010000226         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1005649, whole genome shotgun sequence.
ACCESSION   DASTYP010000226 DASTYP010000000
VERSION     DASTYP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005649"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFV59_09815"
     CDS             complement(<1..573)
                     /locus_tag="VFV59_09815"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEU5326165.1"
                     /translation="MNPSTSDADARFDRTVDRWFRDQLAMHPEWATALGLHDRDAELP
                     PGGRDAIDDEVAFHRRTIDEMSAFDPTELSPERALDRDLAIHESRLRLFHLAEYRAWT
                     GSSSAAADVGEALFPLFTRDFAPLRDRLESIAGRLQAAPRYLGESRERVTDPVRIWTE
                     IDIESTRQLPGFLDTILAAARAETGDDGLVA"
     gene            complement(634..1674)
                     /locus_tag="VFV59_09820"
     CDS             complement(634..1674)
                     /locus_tag="VFV59_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5326166.1"
                     /translation="MPYELPRDPSTARRERISRWIGFAFAAALVVLVAYFGYVGYEGS
                     RQLTDPPNRTGDCRTPADLGWAYRAINYDATGDATMVSPDAPADCDAARAEAGDQVKA
                     PDGIRLAGWYVPAANGDASAPTIVLAHGWGSSKSDMLDRAAPLHDRYDLVLFDFRNHG
                     QSDPATTTQGVAEASDLRAIIDWLVTASGEERIGVLGVSMGGASALAEGARDDRVDAF
                     AIESTHATIGDAAEARIARAGYPLALPGSWAVLLGSLLRTGQDISSVDPLVTITALDE
                     RPVLVVAGGRDDSMGADDPQRLEAAARGAGSPVELHVCEAAGHAESADACPQEYAAWV
                     VGFFDRVFPPSG"
     gene            1712..2203
                     /locus_tag="VFV59_09825"
     CDS             1712..2203
                     /locus_tag="VFV59_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326167.1"
                     /translation="MTSSRRSIPAASRAGAAGRGHHTLAPAHLGEATAAATVAVVVGG
                     IALVITAIGMIVMALTIGARYGADAPPNAGSLAVLPVIGGVVLLLLGIGLTAGGVAVL
                     AHVRRSRLATGILAAVAAALSALGTVVAMINPPADPVIAVALTVAALVFGVAALLLLR
                     PRR"
     gene            2226..2675
                     /locus_tag="VFV59_09830"
     CDS             2226..2675
                     /locus_tag="VFV59_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326168.1"
                     /translation="MARGILLAAAIAWFVAAIGGLVLALAGIDWLLPLLPPLTIDADA
                     VRGAIVTIAAGAGLVAVVHAWTLVGLRVRRRWASTLAILMSALLTVLLFALAVAAVTS
                     VAAQPSAAASLLPAAGAATGGAAAYAVALVRLVQERRAASVSLRGPR"
     gene            2719..2795
                     /locus_tag="VFV59_09835"
     tRNA            2719..2795
                     /locus_tag="VFV59_09835"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2753..2755,aa:Asp,seq:gtc)
BASE COUNT          325 a         1030 c         1031 g          411 t
ORIGIN      
        1 cgccacgagc ccgtcgtcgc cggtctcggc gcgggcggcg gccaggatgg tgtcgaggaa
       61 gccgggcagc tgccgcgtcg actcgatgtc gatctcggtc cagatgcgta cggggtcggt
      121 gacgcgctcg cgactctccc cgaggtaccg gggcgcggcc tgcagccggc cggcgatgct
      181 ctccagccga tcccgcagcg gggcgaagtc acgggtgaac agcgggaaca gggcctcgcc
      241 gacgtcggcc gcagcggacg acgaaccggt ccacgcccgg tactccgcca ggtggaacag
      301 gcgcagccgc gactcgtgga tggcgaggtc acggtcgagc gcacgctccg ggctcagctc
      361 ggtggggtcg aacgccgaca tctcgtcgat cgtccggcgg tggaacgcca cctcgtcgtc
      421 gatcgcgtca cggccgccgg gcggcagctc ggcgtcgcga tcgtggaggc ccagcgcggt
      481 cgcccactcc gggtgcatcg ccagctggtc gcggaaccag cggtcgaccg tccggtcgaa
      541 gcgggcatcg gcgtctgagg tcgacgggtt catcggtcgg cgggtctcct ggcgcgcccg
      601 cgggcgcgca tcggtctgcg gtcggaagcg tcgtcagccg gacggcggga agacgcggtc
      661 gaagaagcct accacccagg cggcgtactc ctgggggcac gcgtcggccg attcggcgtg
      721 gccggcagcc tcgcagacgt gcagctcgac gggcgagccg gcgccacggg cggccgcctc
      781 cagccgttgc gggtcgtcgg cgcccatgct gtcgtcccgg ccaccggcca cgacgaggac
      841 tggccgttcg tcgagggcgg tgatggtcac gagcgggtcc acgctggaga tgtcctgccc
      901 ggtccgcagc agcgagccga ggagcacggc ccagctgccg ggcagcgcca gcgggtagcc
      961 ggcccgggcg atccgcgcct cggccgcgtc gccgatggtg gcatgcgtcg actcgatggc
     1021 gaaggcatcc acgcggtcat cccgcgcgcc ctcggccagg gcggaggcac cgcccatcga
     1081 gacgcccagg actccgatgc gctcctcgcc gctggccgtc acgagccagt cgatgatggc
     1141 ccgcaggtcg ctcgcctcag ccacgccctg cgtggtcgtt gccgggtcgc tctggccgtg
     1201 gttgcggaag tcgaagagga ccaggtcgta ccgatcatgg aggggcgcgg cacgatcgag
     1261 catgtcgctc ttgctgctgc cccagccgtg cgccagcacg atcgtgggcg cggacgcgtc
     1321 gccgttggcc gctggcacgt accagccagc caggcggatc ccgtccggcg ctttgacctg
     1381 gtcgccggcc tcggcccgcg cggcgtcgca atcggccggc gcgtcgggcg agaccatcgt
     1441 cgcatcgccg gtcgcgtcgt agttgatggc ccggtaggcc cagccgaggt cggccggcgt
     1501 tcggcagtcg ccggtgcggt tcggcggatc ggtcagctgg cgcgagccct cgtagcccac
     1561 gtacccgaag taggcgacca ggacgacgag tgccgctgcg aaggcgaagc cgatccagcg
     1621 cgagatgcgc tcccgtcgcg cggtgctcgg atcacgcggc agctcgtacg gcacagcggg
     1681 ccgaggatac tccacgtaca atgcggcgcc gatgacctcc tcacgccgat ccatccccgc
     1741 cgcctcgcgc gccggtgccg ccggacgcgg ccaccacacg ctcgctcccg cgcacctggg
     1801 cgaggcgacc gccgccgcca ccgtggccgt ggtcgtcggc gggatcgcgc tcgtcatcac
     1861 cgcgatcggg atgatcgtca tggccctgac catcggcgct cgctacggcg ccgatgcccc
     1921 gcccaacgcc gggtcgctgg ccgtgctgcc ggtgatcggc ggcgtggtgc tcctgctcct
     1981 ggggatcggc ctcactgccg ggggcgtcgc ggtgctcgcc cacgtccggc gctcgcggct
     2041 cgcgacgggc atcctcgccg cggtcgccgc ggcactgtcg gccctcggca ccgtggtcgc
     2101 gatgatcaac ccgccggcgg acccggtcat cgccgtcgcc ctgaccgtgg ccgcgctcgt
     2161 cttcggcgtc gccgcgctcc tgctgctccg cccccgtcgc tgagcgctcc ctgagggagg
     2221 cccgagtggc gcgtggaatc ctcctcgccg ccgcgatcgc gtggttcgtc gcggccatcg
     2281 gcggcctcgt gcttgcgctc gccggcatcg actggctgct cccgctcctc cccccgctga
     2341 ctatcgacgc ggacgccgtc cgtggcgcga tcgtgacgat tgcggccggc gctggcctgg
     2401 tggccgtggt ccacgcgtgg acgctggtcg gactgcgggt tcggcgtcgc tgggcctcga
     2461 cgctcgcgat cctgatgtcc gcgctgctga cggtgctcct ctttgccctc gccgtggcag
     2521 cggtcacgag cgtcgccgcg cagccgtcgg ccgcggcctc gctgctgccg gccgccggcg
     2581 cggccacggg cggggcggct gcctatgcgg tggcgctcgt gcgactggtg caggagcgac
     2641 gggcggcatc ggtttcgctc agaggccctc ggtgaaggtc gaaccgatct ctgctatgct
     2701 ccgccaccgt cgcgccccgg gcccgtggtg tagcggccta acatgccagc ctgtcacgct
     2761 ggagatcgcc ggttcgaatc cggtcgggcc cgccatc
//
LOCUS       DASTYP010000227         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_862033, whole genome shotgun sequence.
ACCESSION   DASTYP010000227 DASTYP010000000
VERSION     DASTYP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862033"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFV59_09840"
     CDS             <1..550
                     /locus_tag="VFV59_09840"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEU5326169.1"
                     /translation="AFDARCTGIGPSASTSSPPSPDLTAPSPGGAEMAHDVIATCPVC
                     AGELTITRLHCRSCGTALEGEFGVGRFGRLDRDQMALLESFLRSRGNLKEMERELGIS
                     YPTVRGRVDALVRALGFGDQPSEMDLDADDFGTDFDATPEPAIDAEAVAAERRSILER
                     LARKEIGAEEAAAALRALGGTS"
     gene            552..1568
                     /locus_tag="VFV59_09845"
     CDS             552..1568
                     /locus_tag="VFV59_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5326170.1"
                     /translation="MPAFVRTQEIAHELGVAGQFSLRVTSPDVEIRAVEGTVARVRIE
                     YDVRASSEEEADEVLERASFRVRTGAGLLEVSEPKANESGLTSIARALGFGGARVEGR
                     VEAEIPAGTQLSYTGVSADLTAIGLTGAQEYRTVSGDLVLTDVGGRIRISGVSSDVSL
                     RARDPIWLQANTVSGDLSAIAPRIEESRVVTVSGDAELEGALGAGPQHRVETVSGDLM
                     LGLQGGLDLEVRGLSTDVSIGLAHRSEGSRDRRRYHIGDGRAQLLFSSRSGDVTVRPS
                     RRAAPSFPEPPEPPAPPSPPAPPSLDEEEQLRVLQALERGEIDVDEASRRLAGARGQE
                     QTDA"
     gene            1561..2073
                     /locus_tag="VFV59_09850"
     CDS             1561..2073
                     /locus_tag="VFV59_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326171.1"
                     /translation="MPEELETILRLVAEGKLSPDEASPIIEALTRAERRDAEEDLEDA
                     APDVPWRGVERVVRHATRHARRADRHIERAFARAERGLREAELRVGDIGDRLSPGHHL
                     RVRVTERGRQVVNLRVPIGFVESALRFVPGLGGDQAERIRDAVRSGAIGPILDVEDPD
                     GLGGVFISVE"
     gene            2086..>2931
                     /locus_tag="VFV59_09855"
     CDS             2086..>2931
                     /locus_tag="VFV59_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5326172.1"
                     /translation="MTLAIEAVGLTKDFEKGRRTIWQRLRREPDRRETFRAVDGIDLR
                     VEEGEIFGVLGPNGAGKTTTMRMLATLLEPTAGHGTVLGHDLVKDARAIRRRLGAVLS
                     GERSLYWKLTARENLEYFTALYHVPAAETKDRIAGVLETVKLTDRADDYVERFSTGMR
                     QRLVLARALLPNPSLLLLDEPTVGLDPQAARDLRERVLELRAEGRTVLLTTHYMEEAD
                     QLCDRIAIVDHGRIVALDTPEGLKRDLKATEVVHLELSAPDHGEEPAFVGRLNGAGTV
                     THRERV"
BASE COUNT          421 a         1057 c         1089 g          364 t
ORIGIN      
        1 cgcgttcgac gcccgctgca cgggcatcgg gccgagcgcg tctaccagct ccccaccgag
       61 ccccgatctg accgcaccat cgccgggcgg cgctgagatg gcccacgacg tcatcgccac
      121 ctgccccgtc tgcgcgggtg agctgaccat cacgcgactg cactgccgca gctgcggcac
      181 cgctctcgag ggcgagttcg gcgtcggccg ctttgggcgg ctggatcgcg accagatggc
      241 gctcctcgag agcttcctgc gttcgcgcgg caacctcaag gagatggagc gggagctggg
      301 catcagctac cccaccgtcc gcggtcgggt cgacgccctg gtccgcgcgc tcggcttcgg
      361 cgaccaaccg tccgagatgg acctcgatgc cgacgacttc ggcaccgatt tcgacgccac
      421 ccctgaaccg gcgatcgacg ccgaggcggt cgccgcggag cggcgcagca tcctcgagcg
      481 cctcgcgcgc aaggagatcg gcgcggagga ggccgccgcg gcgctgcgtg cgctgggcgg
      541 aacgagctga gatgcccgcc ttcgtccgca cccaggagat cgctcacgag ctcggcgtgg
      601 ccgggcagtt cagcctccgc gtcacgagtc ccgacgtcga gatccgcgcc gtggaaggca
      661 ccgttgcgcg ggtgcgcatc gagtacgacg tgcgcgcgtc ctccgaggag gaggccgacg
      721 aggtgctcga gcgcgccagc ttccgcgtca ggaccggcgc agggctgctc gaggtcagcg
      781 agccgaaggc caatgagagc ggcctcacct cgatcgcccg cgccctcgga tttggcggcg
      841 cacgcgtcga gggccgggtc gaggcggaga ttcccgccgg cacgcagctg agctacacgg
      901 gcgtcagcgc cgacctcacg gccatcggcc tgaccggcgc gcaggagtac cgaaccgtgt
      961 cgggtgacct ggtcctcacc gacgtcgggg ggcgcatccg gatcagcggc gtctcgagcg
     1021 acgtgagcct tcgcgccagg gatccgatct ggctgcaggc caacacggtc agcggcgacc
     1081 tgagcgccat cgcgccgcgc atcgaggaga gccgcgtcgt caccgtcagc ggtgacgccg
     1141 agctggaagg cgcgcttggc gccggcccgc agcaccgggt ggagacggtc agcggcgacc
     1201 tcatgctcgg gctgcagggc ggcctggacc tggaggtccg cggcctgtcg accgacgtct
     1261 ccatcggcct ggcgcaccgc tccgagggat cgcgcgaccg gcgccgctac cacatcggcg
     1321 atggccgggc gcagctgctg ttcagctcga ggtccggcga cgtcaccgtc cggccgtcgc
     1381 ggcgggccgc acccagcttc cccgagccgc cggagccgcc cgctcccccg tcgccgccgg
     1441 cgccaccgtc actcgacgag gaggagcagc tgcgcgtcct gcaggcgctg gagcgcggcg
     1501 agatcgacgt ggacgaggcg tcccgccggc tggccggcgc ccgagggcag gagcagaccg
     1561 atgcctgagg agctcgagac gatcctgcgc ctcgtggccg agggcaagct cagcccggac
     1621 gaggcgtcgc cgatcatcga ggcgctgacg cgcgccgagc gccgggacgc cgaggaggac
     1681 ctcgaggacg cggccccgga cgtcccctgg cggggggtgg agcgcgtggt gcgccacgcc
     1741 acgcggcacg cgcgacgcgc cgaccggcac atcgagcgtg ccttcgcgcg cgccgagcgc
     1801 gggctgcgcg aggcggagct gcgggtcggg gacatcggcg accggctgtc cccgggccat
     1861 caccttcggg tccgcgtcac cgagcgcggc cggcaggtcg tgaacctgcg cgtccccatc
     1921 ggcttcgtgg agtcggcgct gcggttcgtg cccggcctgg ggggcgacca ggcggagcgc
     1981 atccgcgacg ccgtccgctc gggcgccatc ggtcccatcc tggacgtcga ggaccccgac
     2041 ggcctcggcg gcgtcttcat cagcgttgag tgaggagccg gtcgcatgac cctggccatc
     2101 gaggccgtcg gcctgaccaa ggacttcgag aagggacgcc gcaccatctg gcagcggctg
     2161 cgacgcgagc ctgaccgccg cgagacgttc cgcgccgtcg acgggatcga cctgcgggtc
     2221 gaggagggcg agatcttcgg cgttctcggc ccgaacgggg cgggcaagac gaccaccatg
     2281 cggatgctcg ccacgctgct ggaacccacg gcgggccatg gcacggtgct ggggcacgac
     2341 ctggtgaagg acgcgagggc catccggcgc cggctggggg cggtgctgtc gggcgagcgc
     2401 agcctctact ggaagctgac ggcgcgcgag aacctcgagt acttcaccgc gctctaccac
     2461 gtgccggcgg ccgagacgaa ggaccggatc gccggcgtgc tcgagacggt caagctgacc
     2521 gatcgggcgg acgactacgt cgagcggttc agcaccggca tgcgccagcg gctggtgctg
     2581 gcccgagcgc tgctcccgaa cccctcgctg ctgctcctgg acgagccgac ggtcggcctg
     2641 gacccacagg cggcacgcga cctccgcgag cgcgtgctcg agctgcgcgc cgagggccgg
     2701 accgtgctcc tcaccacgca ctacatggag gaggcggacc agctctgcga ccggatcgcg
     2761 atcgtcgacc acgggcggat cgtcgcgctc gacacgcccg agggcctcaa gcgcgacctg
     2821 aaggccacgg aggtcgtcca cctggagctg tcggctcccg accacggcga ggagccggcg
     2881 ttcgtgggcc ggctgaacgg cgcgggcacg gtgacgcacc gcgagcgagt c
//
LOCUS       DASTYP010000228         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_168884, whole genome shotgun sequence.
ACCESSION   DASTYP010000228 DASTYP010000000
VERSION     DASTYP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168884"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFV59_09860"
     CDS             <1..217
                     /locus_tag="VFV59_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU5326173.1"
                     /translation="AASRWGVAVAVVHRYGVVPIGEGAVAIVTAAPHRAEAYEANRFV
                     IEAIKERLPIWKLERFPDGSEWKRPGA"
     gene            complement(214..747)
                     /locus_tag="VFV59_09865"
     CDS             complement(214..747)
                     /locus_tag="VFV59_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU5326174.1"
                     /translation="MQRINVVGTSCSGKTTLARAVAGRLDLPYVELDAHYWGPDWTPV
                     PEAAFHARVRDAVAGERWVIDGGYAPVRHLTWGRADTVVWLDYPLPLVLWRWLRRTVA
                     RVRTGEEFWPGTGNRETIRNVLRRDGLIWYILRTHRRRRRSMSAGLAARPDLRAVRLR
                     SPTEAERWLRSIETRRS"
     gene            941..1999
                     /locus_tag="VFV59_09870"
     CDS             941..1999
                     /locus_tag="VFV59_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326175.1"
                     /translation="MDRDELQDRIRARRSGAEPYRRGVPAEDEELGYQRFEPEPEDTR
                     ARRPRQPETRREDHFREAAPPPPPTSIPAEPPSLTRERDEWEDRPIGAGPSVPPTVVP
                     PAPTPTPVAPIPPMTAAEQPPYEQYEDPEYVDNFDDEAYEGEEYAYAEDWDDGRERRS
                     GAGAFAILGFLALGVIALLGGALLAGIFGGDGGTGQVDTSPSATASAAVSSSAQASQS
                     VMPSASASAGASASAPASGEPVVFPDGFTAEAQPCLPGSAQLDGCDSNASVNSGAVQI
                     WVGFQNGTNQDVIGAALIGPEGNTLGTGSIDLADIDCGSNCGGYTYFNFSNLGPGTYQ
                     VEITRNGQHASSTSFEVS"
BASE COUNT          336 a          799 c          804 g          295 t
ORIGIN      
        1 ggcggcgtcg cgctggggcg tcgcggtggc cgtggtgcat cggtacggcg tcgtgccgat
       61 cggggagggg gccgtggcca tcgtcacggc cgcgcctcac cgggcggagg cgtacgaggc
      121 gaatcggttc gtgatcgagg ccatcaagga gcggctgccg atctggaagc tggagcgctt
      181 cccggacggc agcgagtgga agcggcccgg cgcctagctt cgccgagtct cgatcgaccg
      241 cagccagcgc tcggcctcgg tcggtgaacg caaccggacg gctcggaggt ccggtcgggc
      301 ggcaaggccc gcgctcatgc tgcggcgacg ccgtcggtgg gttctcagga tgtaccagat
      361 caggccgtcg cgccgcagca cgttccggat cgtctcgcgg ttgccggtgc cgggccagaa
      421 ctcctcgccg gtacggactc gggcaaccgt gcggcgcagc cagcgccaca gcacgagcgg
      481 caggggatag tcgagccaca ccaccgtgtc ggcacgcccc cacgtcaggt ggcgcaccgg
      541 tgcgtagccg ccgtcgatca cccatcgctc gccggcgacg gcgtcccgga cccgtgcgtg
      601 gaaggcggcc tcggggactg gcgtccagtc cggtccccag tagtgcgcgt ccagctcgac
      661 gtacggcagg tccaggcggc ccgcgacggc gcgcgcgagg gtcgtcttcc cggagcagct
      721 ggtgcccacg acgttgatgc gctgcatggc gggatgcagc ctagcccgac gggcggggcg
      781 tgcacgcacc ggccgcgcca tgcacaatgc ccgcgatatg gcgacgccga agccgggcga
      841 gatccgctgc ccgacctgcc accgttccac gccgccggca gcgttctgca cgcagtgcgg
      901 ttcggcaatc cctcccgatg cgcgcatccg tccccggggc atggaccgcg acgagctgca
      961 ggaccggatc cgcgcgcgcc gctcgggcgc tgagccgtac cgtcgcggcg tcccggccga
     1021 ggacgaagag ctggggtacc agcgcttcga gcctgagccc gaggacacgc gggctcgtcg
     1081 tccgcggcag ccggagacga gacgcgagga tcatttccgc gaggcggcac caccaccgcc
     1141 gccgaccagc atccccgcgg agccaccgag cctgacccgc gagcgcgacg agtgggagga
     1201 ccgtccgatc ggcgccggcc cgtccgtgcc gccgacggtc gtgcccccgg cacccacgcc
     1261 aaccccggtc gcgccgattc ctccgatgac cgccgcagag cagccaccat acgagcagta
     1321 cgaggacccg gagtacgtcg acaacttcga cgacgaggcg tacgagggcg aggagtacgc
     1381 ctacgcggag gactgggacg acggccgcga gcgccgatcc ggcgccggcg ccttcgccat
     1441 cctcggcttc ctggccctcg gcgtcatcgc gctgctcggt ggtgccctgc tggcggggat
     1501 cttcggcggc gacggcggca ccggccaggt cgacacctcg ccctcggcga cggcgagtgc
     1561 ggctgtgagc tcatcggcgc aggcctccca gagcgtgatg ccgagcgcaa gcgccagtgc
     1621 gggcgccagc gcatcggctc cggccagtgg ggagccggtc gtcttcccgg acggcttcac
     1681 cgccgaagcg cagccttgtc tgcccggcag cgcccaactc gacggttgcg actcgaacgc
     1741 ttcagtgaac agtggtgccg tgcagatctg ggtcggcttc cagaacggca cgaaccagga
     1801 cgtcatcggc gccgcgctca tcgggccaga ggggaacacc ctcggaactg ggtcgatcga
     1861 cctcgcggac atcgactgcg gctccaactg cggcggctac acgtacttca acttctcgaa
     1921 cctcgggccg ggcacgtacc aggtggagat cacgcgcaac gggcagcacg cgagctccac
     1981 cagcttcgag gtttcctgac cgggccgact ccgccagcca cgcctggtgg gcgtgatcgg
     2041 ctcagtggag gccttcgtgt tcgccatccc accacccgcg gtggtgaggg ccgcaccggt
     2101 ggcagagaaa cgcggtcggc gaagcgtcgt tcacgcctcc cagacgtgaa tggcggtgtc
     2161 gcgggctggc gcttcgccgc ccaacggcga caatggtccg cagcgtgcag gtacgggggg
     2221 cgaacccatg accc
//
LOCUS       DASTYP010000229        10011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1663556, whole genome shotgun sequence.
ACCESSION   DASTYP010000229 DASTYP010000000
VERSION     DASTYP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663556"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VFV59_09875"
     CDS             <1..1366
                     /locus_tag="VFV59_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU5326176.1"
                     /translation="NVAMFVDVANMYYAARGQDVDVDYVALLKHATKGRDLIRAYAYT
                     GLDPENENQRKFIDFLGKNGYKPVVKDIRKFGDGRMKANLDIELVVDLFRLADRMDIA
                     VIVSGDGDFAPAIRALQDTGVRCEVVSFKPNTSSDLISVADEFTDIMKIASISRKEGR
                     AVRSMSAPEMPAKEVNPEFGFREASPSVAAAALAAIRSSSDGGRGTTNGRRRSTSRRT
                     EETEPMRADAPVAETDSASESAEETRRRRRRRGGRGRGRGRQESAGESVADETTAPTE
                     GEIGWEEFDDLGQLEGVAVAGEDADEEAVPGFQEADAATLAELGLTPEEIEEMSRGTE
                     AAAVASTEPVAAEATPKRRRSTRARKADEASEAGAATEEAETPKRRRSTRSAKKAEEG
                     ADAAPEAPAAEDAPKRRRSTRAKKSDEASEASATEGDGGEEQPQGIWGRFRSARTTRG
                     GSAS"
     gene            complement(1443..2213)
                     /locus_tag="VFV59_09880"
     CDS             complement(1443..2213)
                     /locus_tag="VFV59_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5326177.1"
                     /translation="MATAPAKTADDLADSIKQLVDRVVDAKFTQEMTKRGQELAGVIA
                     EAVQERGADVTEAAAEAWKDSRPMRRDAAKRMARATNDAAKWSDQTWRKQLRPLVRDL
                     WKRRTVAIGAAGAAVPAGRELVDTAAVRLGLKQREERHWSAFFLGLLIGAAAGAIAAL
                     LTTPKRGSEMRHELATRADEIATKAKDEWVPMFQREEGVGNGHAAESMASIGDASGSV
                     QEGATQGVEAASATADEAAMDTADAINESFDVDREPTA"
     gene            2326..3432
                     /locus_tag="VFV59_09885"
     CDS             2326..3432
                     /locus_tag="VFV59_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326178.1"
                     /translation="MIQSPPDAGFRSVGQATARRIVQLAVEREQLGRTLLVHGPSGAG
                     KDAFVDDLLALFFCTDADRAARPCNACRGCREGRTRRHPDLVLGSPEAWRERKSQGES
                     IVAAARRWLLDAAGAPVVAERRIVLIEHADRASEQIQNALLKSLEEPSDRHTFVLVAD
                     EPAALLPTIRSRCQPLRIGPVPRAELVAHLMDDRRLPQDLAESLARISDGLAGTAEAF
                     VEDRARLDWRRRTQLQLLELLERGRADRFGSVRDLMDETARGMSAPADGGDEDARTPA
                     AVQRVAATAIVDVWLSLARDLLVAGSGRPDLAPGAELSDQLEALAQRIGPGALLEFVA
                     MLERVHEGLAANAAPRLSLEAAMLGWPMLPRTAR"
     gene            3429..3713
                     /locus_tag="VFV59_09890"
     CDS             3429..3713
                     /locus_tag="VFV59_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU5326179.1"
                     /translation="MSGERMTARIRGRVQGVGFRWWVRQRAEALGLTGWVMNADDERS
                     VELVAEGEPTDLDELERLVNRGPQGARVEAVEARRSPATGEFDGFGIVRS"
     gene            3710..4057
                     /locus_tag="VFV59_09895"
     CDS             3710..4057
                     /locus_tag="VFV59_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326180.1"
                     /translation="MNVNWRALGCGLLAAVVFVVVGIFGISRALAPAECPDRLPYQPS
                     AYQPIGEPMSSPALPDTGQLVRGGSASFGLAAWSVWTPAGEAPQASGDPLPERIVLEC
                     GDGTYRAYARGTE"
     gene            4054..4971
                     /locus_tag="VFV59_09900"
     CDS             4054..4971
                     /locus_tag="VFV59_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5326181.1"
                     /translation="MTRRPILLLVNPIAGGKPGSGPGLADDPALLTADALRAALMERG
                     LEVTHRELAEGDDAGELARHAADTGHDVVVAGGDGTVSVAATALVGHADATLGILAMG
                     SFNNMARGFGVPVTLDEALDAIARGGTVSVDAAQVVRSGEEGRTFFEAAGVGLDAVGF
                     LAVEVAEKRGWWRALRALWRGLQLRRTPMRITIDGTAYRTGSPAVTVSNGPYHGMGFA
                     VASEADPTDGELDVAVFHGMGRWEVLRHFLAIARRRPRREPRVGYYRGRRVTVEGVRR
                     ALPAHADGVSIGMTPVTFEVRPGALRIFR"
     gene            5361..6392
                     /locus_tag="VFV59_09905"
     CDS             5361..6392
                     /locus_tag="VFV59_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326182.1"
                     /translation="MASDSTDAAPIPAVWRSNDGATWEEEPYALPTPANGVVEALYQV
                     VATATGFAGLGSAWYGVAMSEDGTTWSYESLGEGACPAAIAANRDVLVAVGSLGPCGI
                     GGVVGTPAIWVRESEGWTLLDTPAAAGWFTAVVATPSGFTAWGPITASADAWVCDIGC
                     RPDPSLEPYAGAPWSSADGRTWRRTNDPAPFAGATIEDIAATDQRTAALGWIWTPDGS
                     SARIALWSSEDGDTWSMVEDLPFEGAPAGPGAGIGATSDVVAVWARGFGAISAITTWT
                     SPDLANWEDDELVADLRRIVGLGDMLMAYGTVPRTSSGETRAPCTKDQVISGTCSNAG
                     AAWVYQVDR"
     gene            complement(6412..7431)
                     /locus_tag="VFV59_09910"
     CDS             complement(6412..7431)
                     /locus_tag="VFV59_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5326183.1"
                     /translation="MQHVGSAGALRRRDREDREAEEERLSPAATRSTQSRGRVTPEEP
                     DEFRTAFERDRDRIIHSKAFRRLKHKTQVFLNPEGDHFVTRLTHTMQVTQVARSLANA
                     LALNEPLTEAIALGHDVGHSPFGHTGEEALSPYFDPDEWHHAAQSVRIFEVLEDQNLT
                     WEVRDGIRAHSWKIDPPPHTPEAYCVRFADRIAYLAHDALDALRGGVLRREDFPPSVL
                     DRFGEPGRPWISSLINAVIDESLRTGAVRMDDETLGVMNELRDFMFAHVYQSAEQLRQ
                     QRRAIAVIRDLMDWHVEHPEEIPESYREHEAPVVVQAADYIAGMTDRYALATHDRLFR
                     PTLNL"
     gene            7718..8659
                     /locus_tag="VFV59_09915"
     CDS             7718..8659
                     /locus_tag="VFV59_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5326184.1"
                     /translation="MLTSPRTGRQHALAVLQALFVTFLWSTSWVLIKIGLDDLDLAPI
                     SFAGLRYALAALVLLPFGIRAVRARADAQRPPDARLFAAVAAYGLLFVAIAQGAQFAA
                     LSVLPATAVTLVLSSTPVWVALIALTAGSERASAMQAAGIGLLLVGAVVYFGPFEVPP
                     DAALGFLAAGVCVAAAATSAHVGRRLARDATLRLGGPVGLTASSMAVGGALLLIVGVL
                     LEGWPSLDLGAWLIVVWLAVVNTAFAFTLWNHTLRTLTAVESSVVNNTMTIQIAVLAV
                     VFLGEALGASQLLGLLLAAVGAAVVQVAPILRGRRRG"
     gene            complement(8656..9543)
                     /gene="phnE"
                     /locus_tag="VFV59_09920"
     CDS             complement(8656..9543)
                     /gene="phnE"
                     /locus_tag="VFV59_09920"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEU5326185.1"
                     /translation="MTAAVAAARPARPSHARRNLALLATAAVVAWSAVTVGIDVTRIV
                     DLPGGLARVFWEMFLKEGVEWSYLGDALGYMLESLQIAWVGTVIGAIISLPLGFFGAK
                     NVSSGLVSNVMRQVLNAIRAFPEIVLAIAIFIPIAGLGPVAGALAIGLHSVGTLGKLT
                     AEVVEGIDPGPVEAARAAGGRPLQVQRWGVVPQVLPEVIAFWLYRFEINIRAAAVLGV
                     VGAGGIGFVLQQAISFGRFPRAGMIILVVVVATILVDTVSGWVRRRIIEGEGAKRVTG
                     DEIEQPELTEDIAARIQRP"
     gene            complement(9540..>10011)
                     /locus_tag="VFV59_09925"
     CDS             complement(9540..>10011)
                     /locus_tag="VFV59_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5326186.1"
                     /translation="FLVDRSILSVVRAIPDILYALVFVAAVGIGPLPGIAALVLFNIG
                     VVAKLLSETVDGVDAGPIEASRAGGGNGVQTMRWAVLPQVLPNYVAYSLYTFELNIRA
                     STVIGIVGAGGIGNLLLGQYRFFAWSNVSVIVVELFVFVFLIEMVSIWLRRRLV"
BASE COUNT         1396 a         3523 c         3652 g         1440 t
ORIGIN      
        1 gaacgtggcg atgttcgtcg acgtcgccaa catgtactac gccgcgcgcg ggcaggacgt
       61 cgacgtcgac tacgtcgccc tgctgaagca cgccaccaag gggcgcgacc tgattcgcgc
      121 ctatgcctac accggtctcg acccggagaa cgagaaccag cgcaagttca tcgacttcct
      181 tggcaagaac ggctacaagc cggtcgtcaa ggacatccgc aagttcggcg acggccggat
      241 gaaggccaac ctcgacatcg aactggtcgt cgacctgttc cggctggcgg atcggatgga
      301 tatcgcggtc atcgtctcgg gtgacggcga cttcgccccc gccatccgcg cgctccagga
      361 caccggcgtc cgctgcgagg tcgtgagctt caagccgaac acctcctcgg acctcatctc
      421 ggtggccgat gagttcaccg acatcatgaa gatcgcctcc atcagccgga aggagggccg
      481 cgccgtgcgc agcatgagcg cgcccgagat gccggccaag gaggtcaacc ccgagttcgg
      541 cttccgcgag gcgtcgccgt cggtcgccgc cgcggccctg gccgccatcc ggtcgtcgtc
      601 ggacggtggc cgcgggacca cgaacggacg ccgtcgctcc acgagccgcc ggacggagga
      661 gaccgagccc atgcgcgccg atgcgcccgt cgcggagacg gactccgcct cggagtcggc
      721 cgaggagacc cgacgccgtc gccgccgccg cggcggacgg ggccgtggac gcggccggca
      781 ggaatcggct ggcgagtcgg tcgccgacga gaccaccgcg ccgaccgagg gcgagatcgg
      841 ctgggaggag ttcgacgatc tcggccagct cgagggcgtt gccgtggccg gggaggacgc
      901 ggacgaggag gccgtgcccg gcttccagga ggccgatgcc gcgacgctcg cggagctcgg
      961 gctgacgccc gaggagatcg aggagatgtc gcgcggaacc gaggcggccg cggtggcatc
     1021 gacggagccc gtcgctgccg aggcgacccc caagcgccgt cgctcgaccc gggccaggaa
     1081 ggccgacgag gcttcggagg caggcgccgc caccgaggag gcggagaccc ccaagcgccg
     1141 tcgctcgacc cggtcggcca agaaggccga agagggtgcg gacgccgcgc ccgaggcgcc
     1201 agccgccgag gacgcgccca agcgtcgtcg ctcgacccgg gccaagaagt ccgatgaggc
     1261 ttcggaggcg agcgccaccg agggtgacgg cggggaggag cagccgcagg gcatctgggg
     1321 ccggttccgg tccgcgcgaa cgactcgggg cggctccgcg tcctgaggac gcggccgccc
     1381 catcacgacg agagccggcc cgtgagggcc ggctcgttcg tgcatggagc tcgcggcgag
     1441 cgctaggcgg tcggctcgcg atccacgtcg aaggactcgt taatggcgtc cgcggtatcc
     1501 atcgcggctt cgtcggccgt cgccgaggcg gcctcgacgc cctgggtggc gccctcctgg
     1561 acgctgcccg aagcgtcacc gatggacgcc atcgactcgg cggcgtggcc gttgcccacg
     1621 ccctcctcgc gctggaacat cggcacccac tcgtccttcg ccttggtcgc gatctcatcg
     1681 gcacgcgtcg ccagctcgtg gcgcatctcc gagccgcgct tgggcgtggt cagcagcgcc
     1741 gcgatggccc ccgctgcggc tccgatgagc aggccgagga agaaagcgga ccagtggcgc
     1801 tcctcgcgct gcttgaggcc cagccgcacc gcggcggtgt cgaccagctc cctgcccgcc
     1861 gggaccgcgg caccggcggc gccgatcgcc acggtgcggc gcttccacag gtcgcggacc
     1921 agcggccgca gctgcttgcg ccacgtctga tcggaccact tggccgcgtc gttcgtcgcg
     1981 cgtgccatgc gcttggcggc gtcgcggcgc atcgggcgcg aatccttcca cgcctcggcc
     2041 gcggcttcgg tcacatcggc gccgcgctcc tgcacggcct ccgcgatcac gcccgcgagc
     2101 tcctggccgc gcttcgtcat ctcctgggtg aacttggcgt cgaccacacg gtcgaccagc
     2161 tgcttgatgg aatcggcaag atcgtcggcc gtcttcgccg gcgcagtcgc catggctacc
     2221 tcctctgact cggccgtctt caggtgcact tagtatgcca ccggcgatct cgccggccag
     2281 tgccgaaggt cgtggccgcc agggcgtccc acctatactc gggccgtgat ccagagccct
     2341 cctgacgccg ggttccgcag cgtcggacag gccaccgcca ggcgcatcgt gcagctcgcc
     2401 gtcgagcgcg agcagctcgg tcggacgctg ctcgtccacg gcccgtcggg cgccggcaag
     2461 gacgcattcg tggacgacct gctggccctc ttcttctgca ccgatgcgga ccgcgccgca
     2521 cgcccctgca acgcctgtcg aggctgccgc gagggtcgca cgcggaggca ccccgacctg
     2581 gtcctcgggt cgcccgaggc ctggcgcgag cgcaagtcgc agggcgagag catcgtggcg
     2641 gccgcgcgcc gctggctgct ggacgcggcc ggcgccccgg tcgtcgcgga gcgacggatc
     2701 gtgctgatcg agcatgccga ccgcgcgtcg gagcagatcc agaacgcgct cctcaagagc
     2761 ctggaggagc ccagcgaccg tcataccttc gtcctcgtcg cggatgagcc cgcggccctg
     2821 ctgcccacca tccgatcgag gtgccagccg ctccgcatcg gtcctgtgcc acgcgcggag
     2881 ctggtggcgc acctcatgga cgatcgacgc ctgccccagg acctcgcgga gtccctcgcc
     2941 cggatcagcg acggtctggc cgggaccgcg gaggcgttcg ttgaggatcg ggctcggctc
     3001 gactggcggc gacgcaccca gctgcagctg ctcgagctgc tcgagcgcgg ccgcgccgat
     3061 cggttcggat ccgtcaggga tctcatggac gagactgcgc gggggatgtc cgcgccggcc
     3121 gacggtggcg atgaggatgc ccgcaccccg gccgcggtgc agcgcgtggc ggcgacggcc
     3181 atcgtcgacg tctggctgtc actcgcccgc gacctcctgg tcgccggctc gggacgcccc
     3241 gacctggcgc cgggtgccga gctcagcgac cagctcgagg cgctcgcgca gcgcatcggc
     3301 ccaggggcgc tgctggagtt cgtcgcgatg ctcgagcgtg tgcacgaggg gctggcggcc
     3361 aacgcggccc cgcggctgag cctcgaggcg gcgatgttgg ggtggccgat gctcccccgg
     3421 acggcgcggt gagcggggag cgcatgacgg cccggatccg aggacgggtt cagggggtcg
     3481 gcttccgatg gtgggtccgg cagcgcgccg aggcgttggg actcaccggc tgggtcatga
     3541 acgcggacga cgagcgctcc gtcgagctgg tggccgaggg tgagccgacg gacctggacg
     3601 agctcgagcg actcgtcaac cgcgggccgc agggcgcgcg ggtcgaggcg gttgaggcgc
     3661 gacgcagtcc ggccacgggg gagttcgacg gcttcggcat cgtgcgttcg tgaacgtgaa
     3721 ctggcgcgcg ctcggctgtg gactgctggc cgccgtcgtc ttcgtcgtcg tcggcatctt
     3781 cggcatctcg cgggcgcttg cgcccgccga atgccccgac cggctgccgt accagccgtc
     3841 ggcctatcag ccgatcggcg agccgatgag ctcgcccgcc ctgccggata ccggccagct
     3901 cgttcgcggg ggttcggcgt cgttcggatt ggccgcgtgg agcgtctgga cgccggccgg
     3961 ggaggcgccg caggcgagcg gcgatccgct gccggagcga atcgtgctgg agtgcggcga
     4021 cggcacctac cgagcgtacg cacgaggcac cgaatgacac gtcgaccgat cctgctcctg
     4081 gtgaacccga tcgccggggg caagcccggc tccggaccgg gcctggccga tgatccggcg
     4141 ctgctcaccg ccgacgccct gcgcgcggcg ctgatggagc gcggcctcga ggtgacgcac
     4201 cgcgaactgg cggaggggga cgatgcgggc gagctggccc gccacgcggc cgacaccggc
     4261 cacgacgtgg tggtcgccgg cggcgacggg acggtcagcg tggcggccac ggcactcgtt
     4321 ggtcacgccg acgcgacgct ggggatcctc gccatgggca gcttcaacaa catggcgcgt
     4381 ggctttgggg tgccggtcac gctcgacgag gcgctggacg cgatcgcgcg cggcgggacg
     4441 gtcagcgtcg atgccgccca ggtcgtccgg tcgggcgagg agggccgcac cttcttcgag
     4501 gccgcgggcg tcggcctcga cgcggtcggc ttcctggccg tcgaggtcgc ggagaagcgt
     4561 ggctggtggc gggcgctgcg cgccctgtgg cgcgggctcc agctgcgacg aacgccgatg
     4621 cggatcacca tcgacggcac cgcctaccgg accggttcgc cggccgtcac cgtcagcaac
     4681 gggccgtacc acggcatggg cttcgcggtc gcgtccgagg ccgatccgac cgacggcgag
     4741 ctcgacgtcg cggtcttcca cggcatgggc cgctgggagg tgctgcgtca cttcctcgcc
     4801 atcgcgcggc gccggccccg tcgcgagcca cgggtcggct actaccgggg gcgccgagtg
     4861 acggtcgagg gggtgcggcg cgcgctgccc gctcacgccg acggcgtgag catcggcatg
     4921 acaccggtca ccttcgaggt tcgtccgggg gcgctccgca tcttccggta gcgcctccta
     4981 cgcggatctc ccgcctgcaa cggagccgat taccttcggc ccttgcccgg ccgaccggtg
     5041 cccgtacgtt gatccggccg cacgtgcagg gaggacaccg cgtgacccgc gtccgcgctg
     5101 cgaccatcgc gatcttcacc gggatctggc tcgcgggatg cgcgagtccg atggcttctc
     5161 cgctcaccac cgcgaaatcc tcacccagcg tcgccccacc gacgcgcgct ccgtctgact
     5221 ccccccgcgc gacccccacg gccagcccga cgcctgccgc cagtcttgct ccgacaggag
     5281 cgtggcggga gctcaccagc gagccgatcc ccttcctgta cgacgttacg gcgaccgcgt
     5341 ccgggttctt cggggtcgga atggcgtccg actcgaccga tgcggcgccg attccggccg
     5401 tgtggcgcag caacgacggg gccacgtggg aggaggaacc ctacgcgctg ccaacgccgg
     5461 cgaacggcgt cgtcgaagcg ctgtatcagg tggtggcaac cgcgaccggg ttcgccggtc
     5521 tcggttcggc gtggtacggc gtcgcgatgt ctgaggacgg cacgacctgg agctacgaat
     5581 cgctgggcga aggcgcatgc cccgcagcca tcgccgccaa ccgcgacgtg ctcgtcgcgg
     5641 ttgggtcgct cggcccgtgt ggtatcggcg gcgtcgttgg cacgcccgcc atctgggtcc
     5701 gcgagtccga gggatggacc ctgctcgaca ctccggcggc cgcgggatgg ttcaccgcgg
     5761 tggtcgcaac gccgagcgga ttcacggcct ggggcccgat cacggcgagc gccgatgcgt
     5821 gggtctgcga catcggctgc cgtccggatc catcgcttga gccctacgcc ggtgcgccgt
     5881 ggtcatcggc ggatggccgc acgtggaggc ggacgaacga cccagcgcca ttcgcgggcg
     5941 ccacgatcga ggacatcgcc gcgacggatc agcggaccgc cgccctcggc tggatctgga
     6001 cgcctgatgg gtcgtccgcc cggatcgccc tctggtcctc cgaggacggg gatacgtggt
     6061 cgatggtcga ggatctgccg tttgagggcg cgccggccgg gccgggcgcc ggaatcggcg
     6121 ccacgagcga cgtcgtcgcg gtatgggcac gtgggtttgg ggcgatctcg gcaatcacca
     6181 cctggacgtc gcccgacctc gccaactggg aggatgacga gctcgtggct gatctgcggc
     6241 ggatcgtcgg tctcggcgac atgctcatgg cctacgggac cgttccacga acatcgagcg
     6301 gggagacccg agcgccctgt acgaaggacc aggtcatctc gggaacgtgc agcaacgcgg
     6361 gcgcggcctg ggtgtaccag gtggatcgct gaggcgaccc gcggtcccgc ctcagaggtt
     6421 caacgtcggg cggaagaggc ggtcatgggt ggcgagggcg tatcggtccg tcatcccggc
     6481 gatgtagtcg gccgcctgca ccacgacggg cgcctcgtgc tcgcggtacg actcggggat
     6541 ctcctcgggg tgctcgacgt gccagtccat caggtcgcgg atgaccgcga tggcgcggcg
     6601 ctgctgccgg agctgctcgg ccgactggta gacgtgcgcg aacatgaagt cgcgcagctc
     6661 gttcatcacg ccgagcgtct cgtcgtccat gcggacggcg ccggtgcgca gtgactcgtc
     6721 gatcacggcg ttgatgaggc tgctgatcca cggtcggccc ggctcgccga agcggtcgag
     6781 gaccgatgga gggaagtcct cgcgacggag gacgccgccg cgcagcgcat ccagcgcgtc
     6841 gtgggcgaga taggcgatcc ggtcggcgaa gcggacgcag taggcctccg gcgtgtgcgg
     6901 cggtgggtcg atcttccacg agtgggcccg gatgccgtcg cgcacctccc aggtcaggtt
     6961 ctggtcctcg agcacctcga agatccgcac cgactgggcc gcgtggtgcc attcgtcggg
     7021 atcgaagtac ggggagagcg cctcctcgcc ggtgtggccg aacggcgagt ggccgacgtc
     7081 gtggcccagc gcgatggcct cggtcagtgg ctcgttgagg gccagcgcgt tggcgaggga
     7141 acgcgccacc tgcgtgacct gcatggtgtg cgtgagccgc gtgacgaagt ggtcgccctc
     7201 cgggttgagg aagacctggg tcttgtgctt caggcgccgg aacgccttgg agtggatgat
     7261 ccgatcgcgg tcgcgctcga aggcggtgcg gaactcgtct ggctcctcgg gggtcacgcg
     7321 gccgcgtgac tgcgtcgacc gcgtggccgc cggactgagg cgctcctctt ccgcctcccg
     7381 gtcctcgcga tcccggcggc gaagggcgcc cgcgctgccg acgtgctgca tgcggccgat
     7441 catgccatgg ggcgcggcgc cagattcccg gcaggcgggc acgtggcggg ttcacggccg
     7501 tccgcactgt cgagttcccg gcattcgggc acggtgacgc ccgtgccagc gccgagatcg
     7561 ccagattccc cgccgacggg tacctgatca cggcgccgcc cggatatcgg gaatctgatg
     7621 ggcgtgcgcc gccacgcacc cggcacggac tcgccacgca cccgccatcc gggaatccga
     7681 cggtgacgtg ctaaggtggc cggctttgtc aggatcgatg ctcacgagcc cccgcaccgg
     7741 ccgccagcac gcgctggcgg tgctgcaggc gctgttcgtc accttcctgt ggtcgacctc
     7801 gtgggtgctg atcaagatcg gcctggacga tctggacctg gcgcccatct ccttcgccgg
     7861 cctgcggtac gcactggcgg cgctggtgct cctgccgttc ggcatccgtg ccgtccgcgc
     7921 ccgcgccgat gcgcagcggc cgcccgacgc gcggctcttc gccgccgtcg ccgcgtacgg
     7981 gctgctgttc gtggccatcg cccagggcgc gcagttcgcg gccctcagcg tgctcccggc
     8041 cacggccgtg acgctggtcc tctcctcgac gccggtgtgg gtggcgctga tcgcgctcac
     8101 ggctggctcc gagcgcgcat cggccatgca ggcggccggc atcggcctgc tgctggtggg
     8161 ggcggtcgtc tacttcggcc ccttcgaggt gccacccgat gcggccctcg gcttcctggc
     8221 cgcaggcgtg tgcgtggcgg cagcggcgac ttccgcgcac gtcggacgcc ggctggcgcg
     8281 cgacgccacc ctccgcctcg gcggaccggt gggactcacc gcgagcagca tggccgtcgg
     8341 tggcgcgctg ctcctgatcg tcggggtgct cctcgagggg tggccctccc tcgacctcgg
     8401 ggcgtggctg atcgtggtgt ggctcgccgt ggtgaacacc gcgttcgcgt tcaccctgtg
     8461 gaaccacacg ctgcgcacgc tgacggcggt ggagtcgagc gtcgtcaaca acaccatgac
     8521 catccagatc gccgtcctgg cggtcgtctt cctcggcgag gccctgggtg cgtcccagct
     8581 gctgggcctg ctcctcgcgg cggtcggagc cgccgtggtc caggtggcgc cgatcctccg
     8641 aggccggagg cggggctagg gccgctggat ccgcgcggca atgtcctcgg tgagctccgg
     8701 ctgctcgatc tcgtcgcccg tgacgcgctt ggcgccctcg ccctcgatga tgcgacgccg
     8761 cacccaaccc gagacggtgt cgaccaggat ggtggcgacg acgaccacca ggatgatcat
     8821 gccggcgcgc gggaatcggc cgaagctgat cgcctgctgc agcacgaagc cgataccgcc
     8881 ggcgcccacg acgcccagca cggccgccgc gcggatgttg atctcgaagc ggtagagcca
     8941 gaaggcgatg acctcgggca gcacctgcgg cacgacgccc cagcgctgga cctggagcgg
     9001 ccggcctccg gcggcgcgcg ccgcctccac cgggccgggg tcgatcccct ccacgacctc
     9061 ggccgtcagc ttcccgaggg tgccgaccga atggaggccg atggcaagcg cgcccgccac
     9121 cggcccgagg ccggcgatcg ggatgaagat ggcgatggcc aggacgatct ccgggaaggc
     9181 ccggatggcg ttgagcacct ggcgcatcac gttcgagacc aggccgctcg agacgttctt
     9241 ggcgccgaag aagccgagcg gcagcgagat gatggcgccg atgacggtgc cgacccacgc
     9301 gatctgcagg gactcgagca tgtacccgag cgcgtcgccg aggtagctcc actcgacgcc
     9361 ctccttgagg aacatctccc agaagacgcg ggccaggccg ccgggaaggt cgacgatgcg
     9421 cgtgacatcg atcccgacgg tgaccgcgga ccaggcgacc accgcggccg tggccagcag
     9481 cgccaggttg cgcctcgcgt gcgagggtcg cgcgggtcgc gcggccgcga cggcggccgt
     9541 cacacgagcc tccgacgaag ccagatcgaa accatctcga tcaggaacac gaagacgaac
     9601 agctcgacga cgatgaccga cacgttgctc cacgcgaaga atcggtactg gccgagcagc
     9661 aggttgccga tgccgccggc cccgacgata ccgatgacgg tcgacgcccg gatgttcagc
     9721 tcgaaggtgt agagcgagta ggcgacgtag ttcggcagca cctggggcag caccgcccag
     9781 cgcatggtct ggacgccgtt gccaccgccg gctcggctcg cctcgatcgg gcccgcatcg
     9841 acgccgtcga cggtctccga cagcagcttc gcgacgacgc cgatgttgaa cagcaccagc
     9901 gcggcgatgc cgggcagcgg cccgataccc accgcggcga cgaagaccag cgcgtagagg
     9961 atgtccggga tggcgcggac gacgctgagg atggaccgat cgaccaggaa c
//
LOCUS       DASTYP010000230         4905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_388174, whole genome shotgun sequence.
ACCESSION   DASTYP010000230 DASTYP010000000
VERSION     DASTYP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388174"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1935
                     /locus_tag="VFV59_09930"
     CDS             175..1935
                     /locus_tag="VFV59_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5326187.1"
                     /translation="MSSCVTARPSRPFLAIGLAALLALTALLAGTPGFTNAAPTATLS
                     SSLSSWLATAADDATSPAIVTFHDRSGMSRLDTLGVGGTRLATLPMAMATLTAAQIRE
                     VASWPEVRSVWHDQEQEPVLDESVPLIGADRVHRGEGLRSPYTGAGIGVAVIDTGMDS
                     THPDLAGAVTYTVVGDPFAADPAVVVPTPTTDTYGHGTHVSSTIAGSGAASGGRFVGV
                     APGATVHSFKTDAGAVLLDGLILASFDYILSHPELGIRVSSNSWGCCDGSDYNPDDPV
                     NVATRAMYDAGITVVFAASNSGGPNTLNPYSASPWVLSVAAGTKDRALADFSSRGRIE
                     GPWDRRAAQRSDTGIYRPSVTAPGQEIEAAKSATATLMAPGVDPEYPMYTYADGTSMA
                     TPHVAGTIALMLDARPTLSPQNVIDIIEGTADNMPAYEMFEVGLGYLDAYESVASAEK
                     GKIHFPPSVNGRTPQFSLASEAAFGGTVLLPNSWALAECPDSTGLLNHHQFTVTSGTD
                     AVYVEIEWADPNQLIYLRLYDPNCQVAGESAALLDIGAVNHRALIVTSPMAGTWTVGV
                     YGRINIPTDYTGSFETYVQN"
     gene            complement(2141..>4905)
                     /locus_tag="VFV59_09935"
     CDS             complement(2141..>4905)
                     /locus_tag="VFV59_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEU5326188.1"
                     /translation="LAALIVLGLLGGAVTLITDVMWFDALGRRDVLVTRLTAQVGLFV
                     AGFVAMLIPALVSVWLARRIAPQAPVRRLGGMEMPDASRLIAIALTAVAILLALGSGA
                     AWSANWETVLLFLNGRGWGVEDPTLGRDVGFYVFDLPFWRFLLGWASTTLIVIGLLTL
                     GTYAARALRWQFHLSAPVRAHLSVIGALLLVVIAGGYQLDIADLAYSTRGVGASVHAA
                     MYTDMNAQLPAYVILTVVALASAALLLLNTWFRTLWLLLLAGGAWIVLSVVVGSLYPS
                     FVQTVQVNPNELNVERPYLVNHLEATRRAFDLDTIDTRRFTGEQELTRDVFDEDQATV
                     DNLRLWDYRPLLTTFGQQQILRRYYAFTDVDIDRYQIAGEQRQIMLSARELDVERLAE
                     PARTWTNERLVFTHGYGITAVPVDAVTPQGTPDYLVSGINREPQLPLQQPRIYFGEAT
                     DTYVVTGTTTAEFDYPIDTGEAGGGETEGAETTWNGTTGVSIGNPIARLLFALRMSDF
                     NLLISGQLTGESQILFDRSIYERVAKIAPFLAYDRDPYLVSADDRLLWVWDAYTVSDR
                     YPNAQPLPAGSRFAGANYVRNSVKVVIDAYDGTVDFYLADPDEPIVAAYARIFPDLFQ
                     PMDAMAEDLRAHLRFPEDLFSAQVEQYRLYHLPANDNGATTFYNQDDRWAIPEDVVAG
                     NDTPMEPYYVIMRIPGEEAAEFVLIQPLVPEGRPNMIAWVAARMDPGVYGERIAFQFP
                     TGTSIPGPVQVESRIAADDAISEQFTLWTNAGSRVVRGNLLVLPIGEDGLLYVEPIFL
                     QAEGAPFPEFVRVIMVDQNRVAFADTVEGAIRQLLGEAEPPPPEQPEPPGESPSPSPS
                     PSPGESPGESPGELPADAAGLIAEAQRLYDEAQAALDDGDLGTYQERIDELARVLDAL
                     EALTGE"
BASE COUNT          753 a         1767 c         1686 g          699 t
ORIGIN      
        1 ccaccgccga tgaccaggat gtccatcgcc ccggagtcta gagcgacgcc gaccggatcc
       61 gggcatgacg attgcccgcc cttcggtcga cccctcgggc acgctccttg cagccacgcg
      121 ggccgcgatc cgagctcccg agccgcggtc aacagacgca caggagttcg ccccatgtcc
      181 tcttgcgtta ccgcgcgtcc ctcgcggccg ttcctggcca tcggcctggc cgcactcctc
      241 gccctgacgg cgctcctggc cggcacgccc ggcttcacga acgcggcgcc gacggcgacc
      301 ctctcctcgt cgctctcgtc ctggttggcg acggccgccg acgacgcgac ctcaccggcc
      361 atcgtcacct tccacgaccg cagcggcatg agccggctcg acaccctcgg cgtcggcggg
      421 acccgcctgg cgaccctccc gatggcgatg gcgacgttga cggcggccca gatccgggag
      481 gtggcatcgt ggccggaggt ccgctcggtc tggcacgacc aggagcagga gccggtcctc
      541 gacgagagcg tcccgctcat cggcgccgat cgggtgcacc gtggtgaggg tctccgttcg
      601 ccctacacgg gcgcgggcat cggcgtggcg gtgatcgaca ccggcatgga ctcgacgcac
      661 ccggacctcg ccggggcagt cacctacacc gtggtcggcg acccgttcgc cgccgatccg
      721 gcggtcgtgg ttccgacgcc gacgaccgac acgtacggcc atggcacgca cgtctccagc
      781 accatcgccg gctcgggcgc cgcgtccggc ggccgcttcg tcggcgtcgc ccccggcgcc
      841 accgtccaca gcttcaagac cgatgccggg gccgtcctcc tcgacggcct gatcctcgcc
      901 tcgttcgact acatcctgag ccacccggag ctcggtatcc gcgtcagcag caacagctgg
      961 ggctgctgcg acggatccga ctacaacccc gacgatcccg tgaacgtggc gacccgcgcc
     1021 atgtacgacg cggggatcac cgtcgtgttc gccgcctcca actcgggtgg gccgaacacc
     1081 ctcaacccgt actccgcgtc gccatgggtc ctcagcgtgg cggccgggac gaaggaccgc
     1141 gccctcgcgg acttcagctc ccgtggccga atcgagggac cgtgggatcg ccgcgcggcc
     1201 cagcggagcg acaccggcat ctaccgaccc tcggtcacgg ccccgggcca ggagatcgag
     1261 gcggccaaga gcgccacggc cacgctgatg gcacccggcg tggatccgga gtacccgatg
     1321 tacacctacg cggacggcac cagcatggcg accccgcacg tggccggaac gatcgccctg
     1381 atgctcgatg cgcgtccgac gctctcgccg cagaacgtca tcgacatcat cgagggcacg
     1441 gccgacaaca tgcccgccta cgagatgttc gaggtcggcc tcggctacct cgacgcctac
     1501 gagtcggtcg catcggccga gaagggcaag atccacttcc cgccctcggt caacggcagg
     1561 acgccgcagt tcagcctcgc cagcgaggcg gcgttcggcg ggaccgtgct gctccccaac
     1621 tcctgggcgc tggccgagtg ccccgactcg acgggcctgc tgaaccacca tcagttcacc
     1681 gtcacctcgg gcaccgatgc ggtctacgtc gagatcgagt gggccgatcc caaccagctg
     1741 atctacctgc ggctgtacga cccgaattgc caggtggccg gtgagtccgc cgcgctcctc
     1801 gacatcggcg cggtgaatca tcgggcgctc atcgtcacct ccccgatggc ggggacctgg
     1861 acggtcggcg tctatggccg catcaacatc ccgacggact acaccgggtc gttcgagacg
     1921 tacgtccaga actgatccgg gccgtcaccg cgcggccaac gtctcgcgca tcggccgtcg
     1981 ggccggggtc cacccccggc ctggcggcca ctccgttgcc tggggcttgc gccttccgag
     2041 gggctggttg cgtggcgcgc cgatcgtggc gcgagcggcc gagctccgag tggaaccacc
     2101 cgagagccgc gaatagtcgc gcctcgcgca accgccgcgg ctactccccg gtcagggcct
     2161 cgagcgcgtc gaggacccgc gccagctcgt cgatccgctc ctggtacgtg cccaggtccc
     2221 cgtcgtcgag cgccgcctgc gcctcgtcgt agagccgctg cgcctcggcg atgaggcccg
     2281 cggcatcggc cggcagctcg ccgggcgact ccccgggcga ctcgcccggc gacggtgacg
     2341 gcgagggcga gggcgactcg cccggcggct ccggctgctc gggcggcggc ggctcggcct
     2401 cgcccaggag ctgccggatg gcgccctcga ccgtatcggc gaaggcgacc cggttctggt
     2461 cgaccatgat cacgcgcacg aactcgggga agggggcgcc ctcggcctgc aggaagatcg
     2521 gctcgacgta cagcagcccg tcctcgccga tgggcagcac cagcaggttg ccgcgcacga
     2581 cacgagagcc ggcgttggtc cacagcgtga actgttcgct gatggcatcg tccgccgcga
     2641 tccgcgactc cacctggacc ggaccgggga tggaggtgcc ggtggggaac tggaacgcga
     2701 tccgctcgcc gtacacgccc gggtccatgc gcgccgcgac ccaggcgatc atgttgggcc
     2761 ggccctcggg gacgagcggc tggatgagga cgaactcagc tgcctcctcg ccggggatcc
     2821 gcatgatcac gtagtacggc tccatgggcg tgtcgttgcc ggcgaccacg tcctccggga
     2881 tggcccaccg gtcgtcctgg ttgtagaagg tcgtcgcgcc gttgtcgttg gccggcaggt
     2941 ggtagaggcg gtactgctcg acctgcgcgc tgaacaggtc ctccgggaag cggaggtggg
     3001 cgcgcaggtc ctccgccatc gcgtccatcg gctggaagag gtccgggaag atgcgggcgt
     3061 aggcggcgac gatcggctcg tccggatcgg ccaggtagaa gtcgacggtg ccgtcgtagg
     3121 cgtcgatcac gaccttcacg ctgttgcgca cgtagttcgc cccggcgaat cgcgatccgg
     3181 cgggcaacgg ctgcgcgttc gggtagcgat cgctgacggt gtaggcgtcc cacacccaca
     3241 gcaggcggtc gtcggcgctg accaggtacg gatcgcggtc ataggcgagg aagggcgcga
     3301 tcttcgcgac gcgctcgtag atggaccgat cgaagaggat ctgcgactcc ccggtcagct
     3361 gcccggagat gagcaggttg aagtcgctca tccgcagcgc gaacagcagc cgggcgatcg
     3421 ggttgccgat gctcacgcca gtcgtgccgt tccacgtcgt ctcggcgccc tcggtctcgc
     3481 cgccgccggc ctcaccggtg tcgatcgggt agtcgaactc cgcggtggtc gtgccggtca
     3541 cgacgtacgt atcggtcgcc tcgccgaagt agatgcgcgg ctgctgcagc ggaagctgcg
     3601 gctcgcggtt gatgccgctg accaggtagt ccggcgtgcc ctgcggcgtg acggcgtcga
     3661 ccggcaccgc ggtaatcccg tagccgtggg tgaagaccag ccgctcgttg gtccaggtcc
     3721 gggccggctc ggccagccgc tccacatcca gctcgcgggc gctgagcatg atctggcgct
     3781 gctcgcccgc gatctggtag cggtcgatgt cgacatcggt gaaggcgtag tagcgccgca
     3841 ggatctgctg ctgcccgaag gtcgtcagca gcgggcggta gtcccacagc cgcaggttgt
     3901 cgaccgtcgc ctggtcctcg tcgaagacgt cgcgggtcag ctcctgctca ccggtgaagc
     3961 ggcgcgtgtc gatggtgtcg aggtcgaagg cgcgacgcgt cgcctcgagg tgattgacca
     4021 ggtacggccg ctcgacgttg agctcgttcg ggttgacctg gaccgtctgc acgaacgacg
     4081 ggtagaggct cccgaccacc accgacagca cgatccacgc gccaccggcc agcagcagca
     4141 gccacagggt ccggaaccag gtgttgagca gcagcagcgc cgccgatgcg agcgccacca
     4201 cggtcaggat cacgtacgcc ggcagctgcg cgttcatgtc cgtgtacatg gccgcatgga
     4261 ccgatgcgcc gacgccccgc gtcgaatacg ccaggtccgc gatgtccagc tggtacccgc
     4321 ccgcgatgac gaccagcagc agcgcgccga tcacggacag gtgcgcccgc accggggcgc
     4381 tcaggtggaa ctgccagcgc agcgcccggg cggcgtaggt gcccagcgtg agcagaccga
     4441 tgacgatcag cgtcgtcgag gcccacccca gcaggaagcg ccagaacggc aggtcgaaga
     4501 cgtagaagcc gacgtcgcga ccaagggtcg gatcctcgac gccccagcca cggccgttga
     4561 ggaagaggag gacggtctcc cagttcgcgc tccacgccgc cccgctgccg agcgccagga
     4621 ggatggcgac ggcggtgagc gcgatcgcga tcagccgcga cgcgtccggc atctccatgc
     4681 cgcccagccg gcgcaccggc gcctgcggcg cgatccgccg ggccaaccag acgctcacca
     4741 gggcggggat cagcatcgcg acgaagccgg caacgaagag gccgacctgc gcggtcaggc
     4801 gcgtcaccag gacgtcgcgc cggccgaggg cgtcgaacca catcacgtcg gtgatgagcg
     4861 tcacggcacc gccgagcagg cccagcacga tgagcgcggc cagga
//
LOCUS       DASTYP010000231        23748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1446156, whole genome shotgun sequence.
ACCESSION   DASTYP010000231 DASTYP010000000
VERSION     DASTYP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 23748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23748
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446156"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..882)
                     /locus_tag="VFV59_09940"
     CDS             complement(88..882)
                     /locus_tag="VFV59_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU5326189.1"
                     /translation="MPRVRLDQLLTERGLARSRAEAQSLILAGEVELDGTRRLKPGQL
                     VSSDAQPTVLQRPRWASRAGAKLEHALDAFGIEPTGMAALDAGASTGGFTDVLLARGA
                     RIVYAVDVGRAQLVDRLRRDPRVVSMERTNLRDLRELPERMNVTTLDLSFIGLALVLP
                     AVRELLATDGEVVALVKPQFEAGKDDVPRGGVVRDPATHARVLRQVITDARRIGLEPK
                     AVERSPIAGADGNVEFLLHLVRGGEPTDLDARVTELTDSAGSEERA"
     gene            complement(954..2906)
                     /gene="dxs"
                     /locus_tag="VFV59_09945"
     CDS             complement(954..2906)
                     /gene="dxs"
                     /locus_tag="VFV59_09945"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU5326190.1"
                     /translation="MTLLETIREPTQLHDMTSDQLDQLAAELREKMIRTVHKTGGHLA
                     SSLGAVEITLALHRVMDSPRDRIVWDTGHQAYAHKLLTGRWDRFDTLRQVGGIGGFPR
                     RSESAHDVFDGGHAGTGVSIGVGLAAARDQKPSTDPARRQRIAVCVGDAAMGSGLTLE
                     ALNHLGHVKHPLLIVLNDNEMSISPSVGGLSTRLNKLRLSRAYQETKTATSRTLPRIP
                     LVGKPALEVLGWAKESFKRSWARVGFFEDIGITYIGVLDGHDREELEEAFEAAFQIDA
                     PVLVHVKTIKGRGYAPAEEDSQSFHGASLPPIDLCLIDPTEEPLPAEATPQRKPKTYT
                     QTFVEELVRLAEADTRITAITAGMPTGTGLSRFAEHFPRRFHDVGIAEQHSVTMAAGL
                     ALAGMRPVVALYSTFSQRAYDGLVHDVCQNDLPVLLALDRSGLVGEDGTSHQGMFMLP
                     AMRSLPNLAIGSPKDEQELRDLVVTALGHPGPMALLYPRDAGEDLDDRAGEALEVGRG
                     EVLREGSDVLLVGFGPIVQRLLAVADTLAADGVDVAVINARWAKPLDESLIAEHAADK
                     RLVVTAEESAGMGGFGDGVLDALNRAGIRAPVHKVALAEGFVHHGAVDDLRRQQRIDA
                     DGILAQVREALGSGESARPRPRVVAG"
     gene            complement(2976..3407)
                     /locus_tag="VFV59_09950"
     CDS             complement(2976..3407)
                     /locus_tag="VFV59_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5326191.1"
                     /translation="MTRIRPARPDDASAIAELTTQLGYPTSTEEAASRLVHLGDGAGV
                     VLVASDEGDTVIGWIQVGAVVTLEVGAHAVIHGLVVGEGHRNARIGAALLEAGEAWAR
                     EAGIAVMLVRSRETRERAHRFYERHGYELTKRSHVFRKPLV"
     gene            complement(3404..3673)
                     /gene="xseB"
                     /locus_tag="VFV59_09955"
     CDS             complement(3404..3673)
                     /gene="xseB"
                     /locus_tag="VFV59_09955"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU5326192.1"
                     /translation="MIDDATLDQLSDEELATRPFDELVAALQQTVQRLEAGSVGLEDS
                     IELYKRGLRLHAACDERLRQAELQITELGRRGVTGAEREPEATSG"
     gene            complement(3670..4962)
                     /gene="xseA"
                     /locus_tag="VFV59_09960"
     CDS             complement(3670..4962)
                     /gene="xseA"
                     /locus_tag="VFV59_09960"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5326193.1"
                     /translation="MSAEQPSLWDQPAADAPPEPPVARPSPRILRVTDLNRRVRSLLD
                     ADAVLADVWVEGEVSQPSFPPSGHCFFTLKDANSQIRCALFREELARVVVRPGHGMNV
                     IVHGRVRAYEPQGVYQLYVESLTPAGAGDLHARYEQLRSRLAAVGLFDDDRKRPIPRW
                     PRRIGVVTSPVGAVWRDINNVLRRRYPLVELVLSPTVVQGATSAPAIERALKRLYAQP
                     DIDTIILARGGGSLEDLWGFNEERVVRTVAESPVPIVVGVGHESDVTLADFAADLRAP
                     TPSAAAELATPDGTQLPAILARLRDRAGAALLGRAQERRRFVDAERRALAGLAPDIIS
                     ARQRAADLLDRGARALSDRAERRRLQLDAAAEGLRTLSPMATLDRGYAIARTADGAIL
                     RDASVVAAGDALQVVLGRGTVETRVERTRTDGTEELLS"
     gene            complement(4987..5547)
                     /gene="efp"
                     /locus_tag="VFV59_09965"
     CDS             complement(4987..5547)
                     /gene="efp"
                     /locus_tag="VFV59_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5326194.1"
                     /translation="MISTGDVKKGVIIELDGQLMKVLDWSHIKMARGSAQVRMKLQNV
                     RKGDIVERTFQAGTRWPRARVEQRKAQYLYSDGDTYHFMDSETYDQFAISGAMLGDDA
                     KYLLENTDVFVSTHESEVLGVDLPVTVDLRVTSTEPGFAGDTATGAKKAATVETGLVV
                     QVPLFVNEGDLLRIDTRTGEYVTRVQ"
     gene            complement(5657..6121)
                     /locus_tag="VFV59_09970"
     CDS             complement(5657..6121)
                     /locus_tag="VFV59_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEU5326195.1"
                     /translation="MNSLVIVGSRSGNTRTIAEAIADGLRAAGPVQITELEEAPTVVP
                     TGTTLLVIGAPTEGHGLYEPMEKYLERLSDEGLAGMEAAAFDTRLDWPRWLSGSAADR
                     IADALRDRGASVLSTESFLVTMKPELEDGQLERARAWGSSLADAVAAAAAAG"
     gene            complement(6237..7331)
                     /locus_tag="VFV59_09975"
     CDS             complement(6237..7331)
                     /locus_tag="VFV59_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5326196.1"
                     /translation="MGDRLSRLRALLDERALDGVLLSRSANKRYHSGFRLGDAEGPTS
                     GWAGTLLVTRDATLIFADSRYTEQAAIEAPDWQLIPTTGPMHVDLPPLLLEHEIAALG
                     MEAAVVSHADWASLAEAAPGVELHAIDDELVPLRILKAPEEVDAIGRACALGDACFAH
                     LVDWIRPGMTEREVAWELESFFRANGAEGLAFDTIALAGPRAAMPHGRPSDAIVEPGN
                     VLLLDFGCIVDGYRSDMTRTLFVGDVPDQIRHYHDAVREAQQAAIDALRPGVDGFDID
                     AIARQRIVDEGVEPYGHGLGHGIGLETHEPPSLRRTAHGSLQPGMVFSVEPGIYLPGV
                     TGIRIEDIVVLEESGPRRLTSALREPIRIG"
     gene            complement(7331..8707)
                     /locus_tag="VFV59_09980"
     CDS             complement(7331..8707)
                     /locus_tag="VFV59_09980"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5326197.1"
                     /translation="MTRRPVMSDYDERRNRRIRELREQREAPVRRRRRVQPLLLLAWI
                     AGSIALLGILIVFGFNVFFAPRVMAWVEQNPGAISNGIVEDFVRWYRPEELADEPASS
                     EIERVTVDVPQGASDAQISKLLYDAGLIKSQIAFHYQVIQAGREGTLNFGTYDLSPTL
                     TPSQIIAAMKGEAVETTTVTIREGLRLEEVVAAVGATDPPMTVNLEELAALLEAPPAD
                     LLNQYDFLADLPAGRSLEGYLSPDTYNMDITWSETKIAQTLLDHFGEQLTQEIRDGIA
                     AKGLTIDEAVIIASIVEREAVIDDERPLIAAVYINRYLNPDNPQTVGLLNADPTLQYG
                     LTTSELRPEGHPLLQGTEGSSLPVDSWGSADWWPMLQTTGGDVALPDSLAGFQTYLNP
                     GIPPMPIANPRPGSIAAVANAPLDQGYLFFVAGCPGGTRDGSHYFAATLDEHNANIAR
                     ANEECAGQ"
     gene            complement(8704..9144)
                     /gene="ruvX"
                     /locus_tag="VFV59_09985"
     CDS             complement(8704..9144)
                     /gene="ruvX"
                     /locus_tag="VFV59_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU5326198.1"
                     /translation="MSRVIALDHGERRIGVAMADRETGMAFARPAIRRRRLEAILTEV
                     AELCAREGAELVVIGLPRNMDGSEGVQAQRARAFGEALAARGLRVVYEDERLSSWDAT
                     QRLAAAGRRPSRASGELDSAAARLILQQYLDARGEGPRHPEETE"
     gene            complement(9141..10814)
                     /locus_tag="VFV59_09990"
     CDS             complement(9141..10814)
                     /locus_tag="VFV59_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEU5326199.1"
                     /translation="MNDDAPALLYLEADDEITAVLRRVRAADPGRVILVAPGRSRATS
                     SVVALRLLAGEGRDLAVVGDALTRSLAAEAGLAAYATVDDARRAAPGEAPPAPEQRQA
                     AIHVVRGDRHDDTAPTLAAAPLVNPEVGEETRPVALSRPPRPQGTVRTPAPPPRRRTR
                     GAPPRRSALIAVLGLLAAFILVASVVGAMVLPAATVTIAPRTEVIGPTTYTIAIDAPR
                     QESGTVTADATVTATGTYAVQQAASGTVVFFNWTFFPVTVPAGTLVAAGEQAFATQAE
                     ITVERGRLTGQGTIRAGEEAVAVVAAAPGAAGNVPAEAINQVLDAETDARLRGFPENT
                     RPTVTNPEPTSGGLDSSGPEFAQADLDAATTSLTDELRQLAAEALPEPGDQMVLPPAT
                     APEPVLTVPEGLVGTRDQPEATISGTLDWSVWLVDRAAVEDEARNRFIGDGASLPDGL
                     QLLSDQTQVDVGDARLLEGDRVEVDVTVTGVATSEIDPAEVIARIRGLGPDEAQLALA
                     DLGPARVDLWPGWVTTVPDMEWRIEVRVEGADLPSGGPSPSDAAASASP"
     gene            complement(10811..12100)
                     /locus_tag="VFV59_09995"
     CDS             complement(10811..12100)
                     /locus_tag="VFV59_09995"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEU5326200.1"
                     /translation="MTAVQGLWSRVTDRVRSAARGRGASPAIASLDIGTEFAKALILT
                     VERDPEGRLVGVVRGSGRQRQGLAHMQSGTVTDIDGVVANCRAALDEAREAAGRRPMA
                     AVIGIAGELVKGTTSKLATRRDDPRRPLDDAELERLVSRAQDRALRDAEERIRWESGV
                     DRLEVRLVHAAIVEMTIDGYPVSNPIGFTGAHVEISVFNAFAPMVHLGALQSVANALG
                     LELLGVIAEPYAVATCLDPGELGDAGAVFVDIGGGTTDVALVRGGGISGTKMLALGGR
                     AFTKGLAERLGLSFADAEAAKLAAADDERPEVAETLLEDARIWRDGVALMIGDLAGDD
                     LLPGRILLCGGGADLPQVRQVLDEGDWWRTLPFARRPQVRAMRTDEVVGLRDATGTLG
                     SRQDVTPMALAHQALIIDAGASAVDRAMRGVVREMRL"
     gene            complement(12111..14738)
                     /gene="alaS"
                     /locus_tag="VFV59_10000"
     CDS             complement(12111..14738)
                     /gene="alaS"
                     /locus_tag="VFV59_10000"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5326201.1"
                     /translation="MQPMSAAEIRERFQRFFEERGHTRVPPAPLLARDDPTLLFLNSG
                     MAQFKRVLTGEEKRDYVRAVDAQPVLRVAGRHNDFEEVGRTPRHNTLFEMLGNWSFGD
                     YFKREAIHWAWEFLTGDLGIPAERLAATVYTDDDVAHDIWASEIGLPPDRLVRWGNIA
                     EGNEQNFWRMGDTGPCGPCSELHFDRGAHLSEGPGCIPDHSETCPRWLEIWNLVFMEF
                     DRAADGTLTPLPFRSVDTGMGLERIAAAIQGVDSNYRTDLFAPIIERLAQFIGHDPET
                     VESERFSYQVVADHSRAMTFLLSEGVTPSNEGAGYVLRRIMRRAVRHGRLLGIERPFL
                     RETCGVVIDLMGGAYPTLAERRDEILDAIETEEDKFARTLEAGSARLAELVDAGGEIS
                     GADAFRLHDTFGFPIDLTIEMAAERGVTVDRAGFDSAMAEQRERSRGEKRGALRLDPE
                     VAALRSEFIGYPNETSADDLRVLALVEAEPMGIVLDRTPFYAEGGGQIGDRGELIGVG
                     GRFRVDDTQRVGDAIVHLGEFVDGTLRPGEAVRGEVDAGRRWASARNHTGTHLLHRAL
                     RDVLGDQAKQAGSWVGPEGLRFDFPAQSATPREALRQVERLVNHQIRRDAPVHPEWMP
                     MQQAQALGADMFFGEKYVPESVRVVRVDGYSKELCGGTHVASTGQIGQLVITGESSIG
                     AGLRRIEAVTGEAATELVADRLEALRSVAQLLGEREENVPQRVEALLSRLREAEKAAK
                     APRATTARLDAAGALHAAQQAGNAKVVVQRYDDADAGALRGLVDDIRGMTGRFVAVLG
                     GVAGDKPSLLVAASRDLAAEGFDSGAIVRAAAGLIGGGGGGRPELAQAGGRDASGLDA
                     ALHEATRLALESLHHLESR"
     gene            complement(14833..15075)
                     /locus_tag="VFV59_10005"
     CDS             complement(14833..15075)
                     /locus_tag="VFV59_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5326202.1"
                     /translation="MKDTGFSSFLAGVVIGGLIGAAIGLLLAPQTGEELREQVGEFVD
                     SKKATFDEAVNEGRAAAEQARAEMVSEHQPAEGQAL"
     gene            complement(15072..15443)
                     /locus_tag="VFV59_10010"
     CDS             complement(15072..15443)
                     /locus_tag="VFV59_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU5326203.1"
                     /translation="MDLALSIFNIGAGLGILAAGGALVYLAWQLTPLIRDSRALTTDL
                     RRLAKTTETELRPILAHTRELTSNAEVLSEDVAVKLDRLTDLMNALQHSLDEGRLTLA
                     SGRPTERPVESWETREDPSEA"
     gene            complement(15462..15740)
                     /locus_tag="VFV59_10015"
     CDS             complement(15462..15740)
                     /locus_tag="VFV59_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326204.1"
                     /translation="MTLGPWLVWVPLLALVNLFVFVAVRGRWGRIVWVLLAASALGVA
                     LGDRIAEATGLEILRIGDMHVVGASVVAQLCMLAVVLLSALGPIRVEE"
     gene            complement(15746..16831)
                     /gene="mnmA"
                     /locus_tag="VFV59_10020"
     CDS             complement(15746..16831)
                     /gene="mnmA"
                     /locus_tag="VFV59_10020"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5326205.1"
                     /translation="MTRIVAAMSGGVDSSVAAALLALERGDDEVIGVWMRTHGDLPEG
                     YELSRSCCSPDAADDARRVAQLLDIPFFILNVEREFGARVIDAFVDAYLDGATPNPCQ
                     ACNQYIKFDELLKRGLAAYGADAVATGHYARLEHRDGRWRLMRAADADKDQTYFLWVL
                     DQAQLARTRFPLGELTKPEVRAIAEKLTLPTAHKPESQEICFVPLGDYRDLLEERRGY
                     AGEPGPIVDGEGQRVGTHTGYAHYTVGQRHGLGVALGEAVYVREVLPSTNTVVIGRRD
                     EVAQRTFSVDGARFTAGEPPAERFRASVRIRHRAPDVPAEVTMLGRDRFLVKTDEPVW
                     APAPGQAAVLYDGEECLGGGRIARTEA"
     gene            complement(16828..17982)
                     /locus_tag="VFV59_10025"
     CDS             complement(16828..17982)
                     /locus_tag="VFV59_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEU5326206.1"
                     /translation="MAIYLDHAATSPLRPEVLEAMLPYLTEHHGNPSSIHGSGRRARQ
                     GLDEARERIAAWMGARAREIVFTSGGTESDNLALKGVAWAASARGRHIVTSPVEHKAV
                     LNAAAVLERQNFEVTVVPVDRYGRVDPADVAAAITDRTTLVSVQAANNEVGTVQPIVE
                     IGALCRERGVLFHVDAVQSAVFAPPAPDAWQAALVSLSAHKLGGPKGVGALYVRQGTA
                     MLPQVNGGSQERQRRAGTENVAGIVGFAAATMASLPVVEVAALRDRLVAGLTALGGVQ
                     LTGHPTERLPNSASVVIDGIEGGDLVAALDLEGIEASTGSACTTGSVEPSHVLLAMGI
                     DAQLAHGSLRLTIGRETTAADVERTLQVTASVVGRMRGAREATAKAVTTA"
     gene            18244..18627
                     /locus_tag="VFV59_10030"
     CDS             18244..18627
                     /locus_tag="VFV59_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEU5326207.1"
                     /translation="MIIRTLEEITGSPNDVGGEGWRARRHFVRADGLGFSMSETTVEA
                     GAEMQLWYKHHNEACYVIEGSAEITERDTGAVHRIGHGDAYAPQHDRHTIRVLEALRL
                     VCVFSPALSGHETHDADGSYEPSRD"
     gene            complement(18807..19244)
                     /locus_tag="VFV59_10035"
     CDS             complement(18807..19244)
                     /locus_tag="VFV59_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326208.1"
                     /translation="MRRLAAALLVGATLAGCGSVIPAGTPHDATAVFRPASMVADRET
                     AAPGDVVELGFPDQMVRGIMFVLEREVGSTWVYRYLLISDANGGEPQWYPADRDDVAV
                     EDVGIAGPGPDRVVIPDVAEPGSWRLCTGNAGENVCVRLEISG"
     gene            complement(19248..21032)
                     /gene="aspS"
                     /locus_tag="VFV59_10040"
     CDS             complement(19248..21032)
                     /gene="aspS"
                     /locus_tag="VFV59_10040"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU5326209.1"
                     /translation="MSTAFRTHTCGELRAADVGQRVTLAGWVHRRRDHGHLAFFDLRD
                     RHGITQVVTNAQEGPEAHAAAEPARNEWVVQAEGVVRHRPDGTTNAELATGEVEVAVD
                     RFTVLNPSKVPPFYINEEQPGLDEGLRLRYRYLDLRRPPMQDRIVLRARLSGAIRRTL
                     EADGFVDVETPTLIRSTPEGARDFVVPSRLQPGEFYALPQSPQQLKQLLMVAGYDRYY
                     QLARCYRDEDLRGDRQPEFTQLDLEMSFVQEADVMAAVERAVIAVSREVTPERPILVE
                     PFPRLTYDEAMARYGSDKPDIRFGMELVELNDAVRGVDFRVFAEPIAAGGAVLGIRAP
                     GCGEYTRKQLDELTDLAKRGGAKGLVHVAVQPDGALHGPAAKFLGSAEASVREALGGA
                     PGDLLLIVADADRIAAAEALGRLRLEMGERLGLRDPSVLAYVWVHRFPMFTWDPEGQR
                     WDATHNPFSAPVPDDVERIESDPGSVRAQQYDLALNGWELGGGSVRIHQRPLLERAFA
                     LMGHSIEGMRDEFGALLDALEYGAPPHGGIALGIDRWAALMANADNIREVMAFPKTQS
                     GSDLMMRAPSPVAPVQLDELHLRVVEED"
     gene            21083..21886
                     /locus_tag="VFV59_10045"
     CDS             21083..21886
                     /locus_tag="VFV59_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326210.1"
                     /translation="MTTMSRAECLQALPRALRSQQSFGPLSLPVTLGLLAGFVASFLA
                     DAFLFDQLRTPWRQAVDGVVFAVVAAPVWIGVQRRDVRSAIDALTWLNGWETERWQRE
                     LGRRLGAVPRATPEILDHLPDTMGLRPLRIELLGVRGELDEAWQRLDELPTDTPWQRF
                     ERAWLNEWLLWLSGGPSQVTEMEAAAGTIPTDDDRALVARTMLAAQQSRRAAVSGGDA
                     VAPLASVRPALGNRPGRYTLGYRTGVIVSVLMVSVVAVAAVMVTSAIIR"
     gene            complement(21876..23162)
                     /gene="hisS"
                     /locus_tag="VFV59_10050"
     CDS             complement(21876..23162)
                     /gene="hisS"
                     /locus_tag="VFV59_10050"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5326211.1"
                     /translation="MARTISAPRGTRDLLPEHTAAWSHVEGVARDLSRRYAFDRLEIP
                     LFERVELFARGLGESSDAVEKEMFRVGGATGSDEERAEWALRPEPTAGIVRAYIEHGL
                     HIRPGPLRVTMIGPMFRYDRPQAGRYRQFTQWDVEVLGDPGPVVDAELIELAHRFYAE
                     VGLHDVVAHVNSIGDAACRPAYRQALIDYFSQHADALSEDARRRLDVNPLRVLDDKQL
                     DDELAAGAPKSTDYLCDACRAHFRTVLDLIDALGLRHVVDHRLVRGLDYYTRTTFEFV
                     IAGREGQQQALGGGGRYDGLAELLGGRPTAGIGFGVGVDRTVLAMEDQGVAVEAGTAL
                     VAVVGTSADDLVDRLRVAAALRDAGLSVRPDGSARKLGKQLESAGKVGARWAVIVGEE
                     TAAWRVGLKNLETGEQETVPVAEVASRVGGANRVSE"
     gene            complement(23173..>23748)
                     /locus_tag="VFV59_10055"
     CDS             complement(23173..>23748)
                     /locus_tag="VFV59_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5326212.1"
                     /translation="TAWPVSVMAEEPSREGRLRAVATGPYGRCVYHADNDVADHQVAT
                     LEFSNGVRATLMVNGLTADNTRTITLNGTRGELRGRLDTGEIEIRRFLPGGETPPGAW
                     DREDAGRSTLRGDETITLTAAPPTVDGHGGGDHAMLDEVIERLRARRDGEVGAAREGR
                     TSLATSLESHLMAFAAEVSRRERRLVDLRGR"
BASE COUNT         3178 a         8694 c         8423 g         3453 t
ORIGIN      
        1 gatgccctcg aagccgatgc ccagctgctc gacggttgga ccggacggtc ctcggcgctt
       61 cacagggctg atcgtagtac gcgcgtgcta cgcccgttcc tcgctcccgg cggaatcggt
      121 cagctcggtc acgcgagcgt ccagatcggt cggttcgccg ccgcgcacga ggtgcagcag
      181 gaactcgacg ttgccgtcag cgcccgcgat gggtgaccgc tcgacggcct tcggctcgag
      241 gccgatgcgt cgagcatcgg tgatgacctg gcgcagcacg cgggcgtgcg tcgctggatc
      301 gcgaacgacg cctccgcgcg gcacatcgtc cttgccggcc tcgaactgcg gcttgaccag
      361 cgccacgacc tcgccgtcgg tggcgagcag ctcgcggacg gccggcagga ccagcgccag
      421 gccgatgaag ctcaggtcca gggtcgttac gttcatccgc tcgggcagct cacgcaggtc
      481 gcgcaggttc gtgcgctcca tgctgaccac ccgcggatct cgcctgagcc ggtccacgag
      541 ctgggcccgg ccgacatcga ccgcgtagac gatgcgagcg ccgcgggcga gcaggacgtc
      601 agtgaagccg ccggtcgagg cgccggcatc cagcgcggcc atcccggtcg gctcgatgcc
      661 gaaggcgtcc agcgcgtgct cgagcttggc gcctgctcgg ctggcccagc gcggcctctg
      721 gaggaccgtc ggctgggcgt cgctgctgac gagctggcca ggcttcagcc ggcgcgtgcc
      781 gtccagctcc acctcaccgg ccaggatcag cgactgcgcc tcggcccgcg agcgtgccag
      841 cccgcgctcg gtgagcagct ggtccaggcg aacgcggggc atgcccgtga tgctagtcgg
      901 tgcggcggtg gagccgcggt cgggtcgttt ctgtgacgcg tatcggtcgc gcgctacccg
      961 gcaaccacgc gcggccgtgg tcgagcgctc tcgccgctgc cgagcgcctc acgaacctgg
     1021 gcgaggattc cgtcggcgtc gatccgctgc tggcgccgca ggtcgtcaac cgccccatgg
     1081 tgcacgaacc cttcggccag cgcgaccttg tggaccggcg cccggatccc ggcacggttc
     1141 agcgcgtcga gcaccccgtc gccgaagccg cccatcccgg ccgactcttc cgccgtcacc
     1201 accagccgct tgtccgcggc gtgctccgcg atcagcgact cgtcgagcgg ctttgcccat
     1261 cgcgcgttga tcaccgcgac gtcgacgcca tcggcggcca gcgtgtccgc cacggccaag
     1321 agccgctgga cgatcggccc gaagccgacc agcagaacat cagacccctc gcgcaggacc
     1381 tcgccgcgcc cgacctccag cgcctcgccc gcccggtcgt ccaggtcctc gcccgcgtcg
     1441 cgcggataga gcagcgccat cggccccgga tgtccgagcg ccgtgaccac gaggtcgcgc
     1501 agctcctgct cgtccttcgg cgagccgatc gccaggttcg gcagcgaccg catggcgggc
     1561 agcatgaaca tgccctggtg cgaggttccg tcctcgccga ccaggccgga tcggtccagc
     1621 gcgagcagca ccggcaggtc gttctggcag acgtcgtgga ccaggccgtc gtacgcgcgc
     1681 tggctgaagg tcgagtacag ggccaccacc gggcgcatgc cggccagcgc cagcccggcg
     1741 gccatggtga ccgagtgctg ctccgcgatc ccgacgtcgt ggaagcgtcg cgggaagtgc
     1801 tccgcgaagc gcgacaggcc ggtgccggtc ggcatgccgg ccgtgatggc ggtgatgcgc
     1861 gtatcggcct ccgccaggcg gacaagctct tcgacgaacg tctgcgtgta cgtcttcggc
     1921 ttgcgctgcg gcgtcgcctc cgcgggcagc ggctcctcgg tcgggtcgat caggcacagg
     1981 tcgatgggcg gcagcgacgc accgtggaag ctctgcgagt cctcctccgc gggcgcgtag
     2041 ccgcggccct tgatggtctt gacgtgcacg agcaccggcg cgtcgatctg gaaggccgcc
     2101 tcgaaggcct cttccaactc ctcgcgatcg tgtccgtcca gcaccccgat gtaggtgatg
     2161 ccgatgtcct cgaagaaccc gacccgcgcc cacgagcgct tgaagctctc cttggcccag
     2221 ccgagcacct cgagtgcggg ctttccgacc agcgggatgc gcggcagcgt gcgcgacgtg
     2281 gccgtcttcg tctcctggta ggcgcgcgac agccgcagct tgttgagccg cgtggagagg
     2341 ccgccgaccg acgggctgat gctcatctcg ttgtcgttga gcacgatcaa cagcgggtgc
     2401 ttgacgtggc cgaggtggtt gagcgcctcg agggtcaggc cggaccccat cgcggcgtcg
     2461 ccgacgcaca cggcgatgcg ctggcgtcgc gccggatcgg ttgacggctt ctggtcgcgg
     2521 gcggccgcca gcccgacccc gatgctgacg cccgtcccgg cgtggccgcc gtcgaagacg
     2581 tcgtgcgcgc tctcgctgcg gcgcgggaag ccgccgatgc cgccgacctg gcgcagcgtg
     2641 tcgaagcggt cccagcggcc cgtcagcagc ttgtgggcgt aggcctgatg cccggtgtcc
     2701 cacacgatcc ggtcgcgcgg cgagtccatc acgcggtgca gcgccagggt gatctcgacc
     2761 gcgcccagcg agctggcgag gtgcccgccc gtcttgtgga cggtccggat catcttctcg
     2821 cgcagctcgg ccgccagctg gtccagctgg tcggacgtca tgtcatggag ctgtgtcggc
     2881 tcgcgaatcg tttcgagcag cgtcaaggga accttgccct ccagagtcgc gggtgagtat
     2941 aggccgggag gcccgaattc gcgtcccggc gaccttcaga cgagcggctt gcggaagacg
     3001 tgactccgct tcgtcagctc gtacccatga cgctcgtaga accgatgcgc ccgctcgcgt
     3061 gtctcccgcg agcgcacgag catgacggcg atgccggcct cgcgcgccca cgcctctccc
     3121 gcctccagta gcgcggctcc gatgcgcgcg ttgcggtgcc cctcgccgac cacgaggccg
     3181 tggatcacgg cgtgggcgcc gacctccagg gtgacgacgg cgccgacctg gatccagccg
     3241 atgaccgtgt cgccctcgtc ggacgcgacc aggaccacac cggccccgtc gccgaggtgt
     3301 acgaggcggg aggccgcctc ttccgtcgag gtcgggtagc cgagctgagt ggtgagctcc
     3361 gcgatggcgg aggcatcgtc cggccgcgcc ggccgaatgc gcgtcatccc gacgtggctt
     3421 cgggctcgcg ttccgcgccg gtcacgccgc ggcgtcccag ctccgtgatc tggagctcgg
     3481 cctggcggag tcgctcgtcg cacgccgcgt gcagccggag cccgcgcttg tagagctcga
     3541 tgctgtcctc cagcccgacg ctgccagcct cgaggcgctg gaccgtctgc tgcagcgcgg
     3601 ccaccagctc gtcgaagggg cgtgtcgcca gctcctcgtc gctcagctgg tccagggtgg
     3661 cgtcatcgat cacgacagca gctcctcggt tccgtcggtg cgggtgcgct ccacgcgcgt
     3721 ctccacggta ccccgcccga gcaccacctg gagcgcgtca ccggcggcga ccaccgaggc
     3781 atcccggagg atggcgccat cggcggtgcg cgcgatcgcg tagccacggt cgagcgtcgc
     3841 catcggcgag agcgtgcgca gaccctccgc cgcggcgtcg agctgaagcc gccgccgctc
     3901 ggcccggtcg ctcagggcgc gggcgccgcg gtcgaggaga tcggcggcac gctggcgagc
     3961 gctgatgatg tccggtgcga ggcccgccag cgcccgccgc tcggcgtcga cgaagcggcg
     4021 ccgctcttgg gcgcgaccca gcagtgcggc tccggcgcgg tcgcgcaggc gggccaggat
     4081 cgcgggcagc tgggttccgt cgggcgtggc cagctcggcg gcggcgctgg gggtcggtgc
     4141 ccgcaggtcg gccgcgaagt cggcgagcgt cacgtccgac tcgtgtccga cgcccaccac
     4201 gatcgggacc ggtgactccg ccacggtgcg caccacccgc tcctcgttga acccccacag
     4261 gtcctccagc gagccgccgc cgcgcgccag gatgatcgtg tcgatgtcgg gctgggcgta
     4321 cagccgcttc agcgcccgct cgatggccgg tgccgatgtc gcgccctgca ccacggtcgg
     4381 cgagagcacc agctcgacca gcgggtagcg tcggcgcagg acgttgttga tgtcgcgcca
     4441 caccgcgccg acgggcgagg tcacgacgcc gatgcgccgc ggccagcgcg ggatcgggcg
     4501 cttgcggtcg tcatcgaaga ggcccaccgc ggccagccgg ctgcggagct gctcgtatcg
     4561 ggcgtgcagg tccccggcgc cggccggcgt gagcgactcg acgtacagct gatagacgcc
     4621 ctgcggctcg taggcgcgga cccggccgtg gacgatgacg ttcatgccgt gcccgggacg
     4681 caccaccacc cgcgccagct cctcgcggaa gagcgcgcat cggatctggc tgttcgcgtc
     4741 cttcaaagtg aagaagcagt gccccgatgg cgggaaggat ggctggctga cctcgccctc
     4801 gacccagaca tcggcgagga ccgcatcggc atcaagcagg gagcggacgc ggcggttcag
     4861 gtcggtgacg cgcaggatgc gcgggctcgg gcgcgcgacg ggaggctcgg gcggcgcatc
     4921 ggccgccggc tggtcccaga gcgagggctg ctcggcgctc atcggtccag cgtagcggac
     4981 ggccgcttac tggacgcggg tgacgtactc gccggttcgg gtgtcgatcc gcagcaggtc
     5041 gccctcgttc acgaacagtg gcacctggac caccaggccg gtctcgacgg tggcggcctt
     5101 cttggcaccg gtggccgtat ctcccgcgaa cccgggctcg gtcgacgtga cgcgcaggtc
     5161 gacggtgacg ggaaggtcga cgcccaggac ctcgctctcg tgggtgctga cgaagacgtc
     5221 ggtgttctcg agcaggtact tcgcgtcgtc acccagcatg gcgccgctga tcgcgaactg
     5281 gtcgtacgtc tccgaatcca tgaagtggta cgtgtcgccg tcgctgtaca ggtactgcgc
     5341 cttgcgctgc tcgacgcgcg ctcgcggcca gcgggtgccg gcctggaagg tgcgctcgac
     5401 gatgtcgccc ttgcgcacgt tctgcagctt catgcgcacc tgcgccgaac cgcgggccat
     5461 cttgatgtgg ctccagtcca ggaccttcat gagctgcccg tcgagctcga tgatgacgcc
     5521 cttcttgacg tcgcccgtgc tgatcaatcg cgtctcctcg tggggtggcc gatgcggccc
     5581 acaggatagc gcggcgctcc ccccggaggc ggtgcgtata tcggtatata cgagggcgtg
     5641 cgcgacggcg cgcccgtcag ccggccgctg cggccgccgc cacggcatcg gccagggagc
     5701 tgccccacgc tcgtgcccgc tccagctgcc cgtcctcgag ctccggcttc atcgtgacca
     5761 ggaagctctc ggtggacagg acggatgcgc cgcggtcgcg cagggcgtcg gcgatgcgat
     5821 ccgccgcgga gcccgacagc cagcgtggcc agtcgagccg cgtatcgaac gcggccgcct
     5881 ccatgcccgc cagcccttcg tcgctcaacc gctcgagata cttctccatc ggctcgtaga
     5941 ggccgtggcc ctcggtcggg gcaccgatca ccagcagcgt ggtgccggtt ggcacgaccg
     6001 ttggcgcctc ctcgagctcg gtgatctgga ccgggccggc ggcgcgcagg ccgtcggcga
     6061 tggcctcggc gatcgtgcgc gtattcccgg agcgcgaccc gacgatgacg agactgttca
     6121 ttgctgctgc acctcctgcg tctgcgagcg tccacgtccg gacgtcgcgg tggcagggcc
     6181 ggagggaccg atcgggacgg gccgtgcgtc ccgggccgcg cggtcgagac ctggcgtcag
     6241 ccgatgcgaa tgggctcgcg cagtgcgctc gtcaggcgtc gggggccgga ctcctcgagc
     6301 acgacgatgt cctcgatccg aatcccggtg actcccggca ggtagatccc cggctcgacg
     6361 ctgaacacca tccccggctg caggctcccg tgcgccgttc gccgcagcga gggcggctcg
     6421 tgggtctcga ggccgatgcc gtggccgagc ccgtgcccgt agggctccac gccctcgtcc
     6481 acgatccgct gacgggcgat ggcgtcgatg tcgaagccgt cgaccccggg ccggagcgcg
     6541 tcgatggccg cctgctgggc ctcgcgcacg gcgtcgtggt agtggcgtat ctggtccggc
     6601 acatcgccca cgaacagggt gcgggtcatg tcggaccggt acccgtcgac gatgcagccg
     6661 aagtcgagta gcaggacgtt cccgggctcc acgatcgcgt cggatgggcg gccgtgcggc
     6721 atggcggctc ggggtccggc cagcgcgatc gtgtcgaagg ccagcccttc ggcgccgttg
     6781 gcgcggaaga acgactccag ctcccaggcc acctcgcgct cggtcatgcc ggggcggatc
     6841 cagtcgacca ggtgcgcgaa gcaggcgtcg cccagcgcac aggcgcgccc gatcgcatcc
     6901 acctcctcgg gcgccttgag gatccgcagc gggaccagct cgtcgtcgat ggcgtggagc
     6961 tcgacgccgg gtgccgcctc ggccagcgac gcccaatcgg cgtgcgagac gacggccgcc
     7021 tccatgccga gcgccgcgat ctcgtgctcc aggaggagcg gcgggaggtc gacgtgcatc
     7081 ggtcccgtgg tcgggatgag ctgccagtcc ggtgcctcga tcgccgcctg ctcggtgtag
     7141 cgggagtcgg cgaagatcag cgtcgcgtcg cgcgtgacga gcagcgtgcc ggcccagccc
     7201 gaggtcggac cctcggcatc gccgagccgg aagccgctgt ggtagcgctt gttggcgctg
     7261 cggctcagca gcacgccatc cagcgcgcgc tcgtcgagga gcgcccggag gcgcgacagc
     7321 cgatccccca tcactggccg gcgcactcct cgttggcccg tgcgatgttc gcgttgtgct
     7381 cgtcgagcgt tgccgcgaag tagtgcgagc cgtcgcgcgt gccgcccggg cagccggcca
     7441 cgaagaacag gtagccctga tcgagcggtg cgttggccac ggccgcgatc gagccgggac
     7501 gcgggttggc gatgggcatc ggcgggatgc ccgggttgag gtacgtctgg aagccggcca
     7561 gggagtccgg gagcgcgacg tcgccgccgg tggtctggag catgggccac cagtcggcgc
     7621 tgccccacga atcgaccggc agggagctgc cttcggtgcc ctgcaggagc ggatggccct
     7681 ccggccgcag ctcggacgtc gtcagcccgt actgcagcgt cgggtccgcg ttgagcaggc
     7741 ccaccgtctg cgggttgtcc gggttgaggt accggttgat gtagacggcg gcgatcagcg
     7801 gtcgctcgtc gtcgatcacc gcctcacgct cgacgatgct ggcgatgatc accgcctcgt
     7861 cgatggtcag gcccttggcc gcgattccat cgcggatctc ctgcgtcagc tgctcgccga
     7921 agtggtcgag gagggtctgc gcgatcttcg tctccgacca ggtgatgtcc atgttgtacg
     7981 tgtcgggcga gaggtagccc tccagcgacc ggccggcggg cagatcggcc aggaagtcgt
     8041 actggttcag caggtccgct ggcggcgcct ccagcagggc ggcaagctcc tcgaggttga
     8101 cggtcatcgg cggatcggtc gcgccgacgg cggcgaccac ctcctccagt cgcaacccct
     8161 cgcggatggt gacggtcgtc gtctccaccg cctctccctt catggccgcg atgatctggc
     8221 tgggcgtcaa cgtcggcgac aggtcgtagg tgccgaagtt gagcgtgccc tcgcggccgg
     8281 cctggatgac ctggtagtgg aacgcgatct ggctcttgat caggcccgcg tcatacagca
     8341 gcttcgagat ctgcgcgtcc gatgcgccct gcggcacgtc cacggtgaca cgctcgatct
     8401 ccgagctggc gggctcgtcg gcgagctcct cggggcgata ccagcgcacg aagtcctcga
     8461 cgatgccgtt gctgatggcg cccgggttct gctcgaccca cgccatcacc cgcggggcga
     8521 agaacacgtt gaagccgaac acgatgagga tgccgaggag cgcgatgctg ccggcgatcc
     8581 aggccagcag caggagcggc tgcacgcgcc ggcgacgtcg gacgggcgcc tcgcgctgct
     8641 cgcgcagctc gcggatgcgc cggttgcggc gctcgtcgta gtcgctcatg acggggcgtc
     8701 gggtcattcg gtctcctcag ggtgccgcgg gccctcgcca cgggcgtcca ggtattgttg
     8761 caggatcagc cgggccgccg ccgagtccag ctcgccgctg gcgcggctcg gccggcgacc
     8821 tgcggcagcc agccgttggg tcgcgtccca cgagctgaga cgctcgtcct cgtacaccac
     8881 gcgcaaccca cgggccgcca gcgcctcccc gaacgcgcgg gcccgctgcg cctgcacccc
     8941 ctccgagccg tccatgttcc gcggcagtcc gatgacgacg agctccgccc cctcccgggc
     9001 gcacagctcg gccacctcgg tgaggatggc ctccagccgg cgccgtcgga tcgcgggtct
     9061 ggcaaacgcc atgccggttt cgcggtcggc catcgccaca ccgatgcgcc gctcgccgtg
     9121 gtcgagcgcg atcacccggc tcacggcgat gcgctggcgg cggcgtccga gggggacggg
     9181 ccgccgctcg gcagatcggc gccctccacg cgcacctcga tccgccactc catgtccggg
     9241 acggtcgtga cccagccggg ccacaggtcg acccgcgccg gtccgaggtc ggccagggcc
     9301 agctgcgcct cgtccggccc caggccgcgg atgcgcgcga tgacctcggc cggatcgatc
     9361 tcggaggtcg ccaccccggt gacggtcacg tcgacctcga cgcggtcgcc ctcgaggagc
     9421 cgggcgtcac ccacgtcgac ctgcgtctgg tcggacagga gctgcaggcc gtccggcagc
     9481 gacgcgccgt cgccgatgaa ccgattgcgc gcttcgtcct ccacggcggc acggtcgacc
     9541 agccagaccg accagtcgag cgtgccggag atggtcgcct cgggctggtc gcgcgtgccc
     9601 accaggccct ccggcacggt gaggaccggc tcgggtgcgg tggctggcgg gagcaccatc
     9661 tggtcgccgg gttcgggaag cgcctccgcg gcaagctgcc ggagctcatc ggtcagcgac
     9721 gtcgtggcgg cgtcgagatc ggcctgcgcg aactccgggc cgctgctgtc gagcccgccg
     9781 gacgttggtt ccgggttcgt caccgtcggg cgcgtgttct ccgggaagcc acgcaggcgc
     9841 gcatcggtct cggcgtcgag cacctggttg atggcctcgg ccggcacgtt gccggccgcc
     9901 ccgggtgcgg ccgcaaccac cgccaccgcc tcctcaccgg cgcggatggt gccctgtccg
     9961 gtcagccgtc cacgctccac ggtgatctcc gcctgcgtgg cgaacgcctg ctcccccgcc
    10021 gccaccagcg ttccggccgg caccgtgacc ggaaagaagg tccagttgaa gaagacgacc
    10081 gtgccggacg ccgcctgctg aacggcgtac gtcccggtgg cggtgaccgt cgcgtccgcc
    10141 gtgaccgtgc cggactcctg gcgcggcgcg tcgatggcga tggtgtaggt ggtgggtccg
    10201 atcacctccg tccgcggggc gatggtgacc gtggccgccg gcaagaccat ggcgccgacc
    10261 acgctggcga ccaagatgaa ggcggccagc agcccgagca ccgcgatcag cgccgagcga
    10321 cgcggaggcg cgccacgcgt ccgccgtcga ggaggtggtg cgggcgtgcg caccgtcccc
    10381 tgtggccgcg gcgggcgact cagcgcaacc ggacgggtct cctcccccac ctccgggttg
    10441 acgagcggcg cggcggcgag cgtcggcgcc gtgtcgtcgt ggcggtcgcc tcggacgacg
    10501 tggatggccg cctgccgctg ctcgggcgcg ggcggggcct cgccgggcgc cgcgcggcgt
    10561 gcgtcgtcga cggtggcgta ggccgccagc ccggcctcgg ccgccaggga gcgggtcagc
    10621 gcgtcgccca ccaccgccag gtcgcgccct tcgccggcga ggagccggag ggcgaccacg
    10681 ctggaggtcg cgcgcgaccg gcccggcgcg accaggatga cgcggccggg atcggccgcc
    10741 cggacgcgcc gcaggacggc cgtgatctcg tcgtcggcct cgaggtagag cagggccggg
    10801 gcgtcgtcgt tcacagccgc atctcgcgca cgacgccgcg catcgcgcgg tccaccgctg
    10861 acgcgccggc gtcgatgatc agggcctggt gggccagtgc catgggcgtc acgtcctggc
    10921 ggctgccgag ggtgccggtg gcgtcgcgca ggccgaccac ctcatcggtc cgcatggcgc
    10981 gcacctgcgg acggcgggcg aacggcaggg tccgccacca gtcgccctcg tcgagcacct
    11041 ggcgcacctg cggcaggtcc gcaccgccac cgcacagcag gatccttccc ggcagcagat
    11101 cgtcgccggc gaggtcgccg atcatcagcg ccaccccgtc gcgccagatg cgggcgtcct
    11161 ccagcagggt ctcggccacc tcgggtcgct cgtcgtcggc cgcggccagc ttggccgcct
    11221 ccgcatcggc gaaggagagg cccaggcgtt cggcgaggcc cttggtgaag gcgcgcccgc
    11281 cgagcgccag catcttcgtt ccgctgatgc cgccgccgcg caccagcgcc acgtccgtcg
    11341 tcccgccgcc gatgtccacg aacacggcgc ccgcatcgcc cagctcaccg ggatccaggc
    11401 aggtggcgac ggcgtacggc tcggcgatga cgccgagcag ctcgaggccg agggcgttgg
    11461 ccaccgactg cagcgcaccc aggtggacca tcggcgcgaa ggcgttgaag acgctgatct
    11521 cgacgtgcgc gcccgtgaag ccgatggggt tgctgaccgg gtatccgtcg atggtcatct
    11581 cgacgatggc ggcgtggacg agccgcacct cgagccgatc cacgcccgac tcccagcgga
    11641 tgcgctcctc ggcgtcgcgc agggctcggt cctgggctct cgagaccagg cgctcgagct
    11701 cggcatcgtc cagggggcgc cgggggtcgt cacgccgcgt ggcgagcttc gaggtcgtgc
    11761 ccttgaccag ctcgcccgcg atgccgatga ccgccgccat gggacgtcgg ccggcggcct
    11821 cgcgcgcctc gtcgagcgcc gcccggcagt tcgccaccac gccgtcgatg tccgtcaccg
    11881 tgccggattg catgtgcgcc agcccctggc gctgccggcc ggagccgcgc acgacgccga
    11941 ccagcctccc ctcgggatcg cgctcgaccg tgaggatgag cgccttggcg aactcggtcc
    12001 cgatgtcgag cgacgcgatc gccggggatg cgccacgtcc gcgggcggcg ctccgcaccc
    12061 ggtcggtgac gcgactccag aggccctgaa ccgcggtcac gacccgctgg tcagcgtgac
    12121 tcgaggtggt ggagcgactc cagcgccagc cgggtcgcct catgcagcgc cgcgtcgagc
    12181 cccgaggcgt cgcgcccgcc cgcctgggcc agctccggcc gtccgccgcc accgccgccg
    12241 atcaatcctg ccgccgcgcg tacgatggcg ccgctgtcga agccctccgc ggccaggtcg
    12301 cgcgaggcgg cgacgaggag cgacggcttg tcgccggcca cgccgcccag gaccgccacg
    12361 aaccgtccgg tcatgccccg gatgtcgtcg acgagcccgc gcagcgcgcc cgcatcggcg
    12421 tcgtcgtagc gctggacgac gaccttcgcg ttgcccgcct gctgcgccgc gtgcagcgcg
    12481 cccgccgcgt ccagccgggc ggtggtcgcc cggggcgcct tggccgcctt ctccgcctcg
    12541 cgcagccggg agagcagcgc ctcgacacgc tgggggacgt tctcctcccg ctcgccgagc
    12601 agctgggcca cgctccgcag cgcctcgagc cggtccgcca cgagctcggt ggccgcctcg
    12661 ccggtcaccg cctcgatccg gcgcaggccg gcgccgatgc tcgactcgcc ggtgatgacc
    12721 agctgcccga tctgccccgt cgaggcgacg tgcgttccgc cgcacagctc cttggagtac
    12781 ccgtcgaccc ggacgacgcg cacggactcg gggacgtact tctcgccgaa gaacatgtcg
    12841 gcgccgagcg cctgggcctg ctgcatcggc atccactcgg ggtggacggg ggcgtcgcgc
    12901 cggatctggt ggttgacgag ccgctcgacc tgacggagcg cctcgcgagg cgtcgccgac
    12961 tgggccggga agtcgaaccg cagcccctcg gggccgaccc acgagccggc ctgcttggcc
    13021 tgatccccca ggacgtcgcg cagcgcccga tgcagcagat gcgtgcccgt gtggttgcgc
    13081 gccgacgccc agcgccgccc ggcgtcaacc tcgccgcgca cggcctcgcc gggccgaagc
    13141 gtcccgtcca cgaactcacc caggtgcacg atcgcgtcgc ccacgcgctg cgtgtcgtcg
    13201 acccggaagc gcccgccgac gccgatcagc tccccgcggt cgccgatctg cccgccgccc
    13261 tcggcgtaga acggggtgcg gtcgagcacg atccccatcg gctcagcttc gaccagggcg
    13321 aggacgcgca ggtcgtcggc actggtctcg ttcgggtagc cgatgaactc gctgcgcagg
    13381 gcggcgacct cagggtccag ccgcagggcg ccgcgcttct cgccgcgcga gcgctcgcgc
    13441 tgctcggcca tcgcgctatc gaagccggca cggtcgaccg tgacgccgcg ctcggcggcc
    13501 atctcgatcg tcaggtcgat cgggaagccg aaggtgtcgt gcaggcggaa cgcatcggcc
    13561 ccgctgatct cgcccccggc gtcgaccagc tcggccaggc gtgcactgcc ggcctccagc
    13621 gtccgcgcga acttgtcctc ctcggtctcg atcgcatcga ggatctcgtc gcgccgctcg
    13681 gccagcgtcg ggtaggcgcc gcccatcagg tcgatgacca cgccgcaggt ctcgcgcagg
    13741 aacggccgct cgatgccgag cagtcggccg tggcggaccg cgcggcgcat gatgcggcgc
    13801 agcacgtagc ccgcgccctc gttcgagggc gtgacgccct cggacagcag gaacgtcatg
    13861 gcgcgcgagt ggtcggccac cacctggtag ctgaagcgct ccgactccac ggtctccggg
    13921 tcgtgaccga tgaactgcgc caggcgctcg atgatcgggg cgaacaggtc ggtccggtag
    13981 ttggagtcga ccccctggat ggccgcggcg atgcgctcga gccccatccc ggtgtcgacg
    14041 ctcctgaacg gcagcggcgt gagcgtgccg tcggccgcgc ggtcgaactc catgaagacc
    14101 aggttccaga tctccagcca gcgggggcac gtctccgagt ggtcagggat gcagcccggt
    14161 ccctcggaca ggtgggcgcc gcggtcgaag tgcagctcac tgcacgggcc gcaggggccg
    14221 gtgtcgccca tgcgccagaa gttctgctcg ttgccctccg cgatgttccc ccagcgcacc
    14281 agccgatccg gcggcaggcc gatctcggag gcccagatgt cgtgggcgac gtcgtcatcg
    14341 gtatagacgg tcgcggccag gcgttcggcc gggatgccca ggtcgccggt caggaactcc
    14401 caggcccagt ggatcgcctc gcgcttgaag tagtcgccga agctccagtt gcccagcatc
    14461 tcgaacagcg tgttgtgccg cggcgtacgg ccgacttcct cgaagtcgtt gtgccggccc
    14521 gcgacacgga ggacgggctg ggcatcgacg gcccggacgt agtcgcgctt ctcctcgccg
    14581 gtcagcacgc gcttgaactg cgccatgccg gagttgagga agaggagcgt ggggtcgtcg
    14641 cgggcgagca gcggcgccgg aggaacgcgc gtatggccgc gctcctcgaa gaatcgctgg
    14701 aatcgctcgc ggatctcggc cgcgctcatc ggctgcatcg ggtgtctcgg ttcgtccatc
    14761 cgttgatcac gacgagagcc ggcgacccac cggctctcga ggctgctctg gtaacgggtc
    14821 ggttgtgggt gcctacaggg cctgaccctc ggccggctga tgctcggaca ccatctcggc
    14881 gcgcgcctgc tcggcagctg cccggccctc gttgaccgcc tcgtcgaagg tcgccttctt
    14941 gctgtccacg aactcgccga cctgctcgcg cagctcctcg cccgtctgcg gcgccagcag
    15001 cagcccgatg gcagcgccga tcagtccgcc gatgaccacg cccgccagga agctgctgaa
    15061 tccggtgtcc ttcatgcctc ggaggggtcc tctcgcgttt cccacgattc taccggccgc
    15121 tcggtcggcc gtcccgatgc gagggtcaga cgcccctcgt ccaggctgtg ctggagcgcg
    15181 ttcatgaggt cggtgagccg gtcgagcttg acggccacgt cctcgctcag gacctcggcg
    15241 ttgctggtca gctcgcgggt gtgggccagg atcgggcgca gctcggtctc ggtcgtcttc
    15301 gccagtcgcc gcaggtcggt cgtcagggcg cgcgagtcgc ggatgagcgg cgtcagctgc
    15361 caggccaggt aaaccagcgc gccaccggcg gccaggatgc cgagcccggc gccgatgttg
    15421 aagatggaca gggcgagatc catggccgcg actctacccc cttactcctc cacgcggatc
    15481 ggcccgagcg cgctcagcag cacgacggcc agcatgcaca gctgcgccac caccgatgcg
    15541 cccaccacgt gcatgtcacc gatgcgcagg atctcgaggc ccgtcgcttc cgcgatccga
    15601 tcgccgagcg ccacgcccag cgccgacgcg gcgagcagca cccagacgat gcgcccccac
    15661 cggccgcgga cggccacgaa cacgaacagg ttgacgagcg ccagcagcgg cacccacacg
    15721 agccacggcc cgagcgtcac cgccgtcagg cctcggtccg cgcgatgcga cccccgccga
    15781 ggcattcctc gccgtcgtac agcacggccg cctggccggg tgcgggcgcc cagaccggct
    15841 cgtcggtctt caccaggaag cggtcgcggc cgagcatggt gacctcggcc ggcacgtccg
    15901 gagctcggtg gcggatgcgg accgatgcgc ggaagcgctc ggccggtggc tcgccggccg
    15961 tgaaccgcgc gccgtcgacg ctgaacgtcc gttgcgcgac ctcgtcgcga cggccgatga
    16021 cgaccgtgtt cgtggacggc aagacctcgc ggacgtagac cgcctcaccg agcgcgaccc
    16081 ccagcccatg gcgctgcccg acggtgtagt gggcatagcc ggtgtgcgtg cccacccgct
    16141 gcccctcccc gtcgacgatc ggccccggct cgccggcgta cccgcgccgc tcctcgagca
    16201 ggtcgcggta gtcgcccagg gggacgaagc agatctcctg gctctcgggc ttgtgggcgg
    16261 tcggcagcgt cagcttctcg gcgatggcgc ggacctccgg cttggtgagc tcgcccagcg
    16321 ggaatcgggt gcgcgccagc tgcgcctggt cgagcaccca caggaagtag gtctggtcct
    16381 tgtcggcatc ggcggcgcgc atcaggcgcc agcggccgtc gcggtgctcg agacgggcgt
    16441 agtggccggt ggcaaccgca tcggcgccgt aggcggcaag gccgcgcttc aagagctcgt
    16501 cgaacttgat gtactggttg cacgcctggc acgggttcgg cgtcgctccg tccaggtagg
    16561 cgtccacgaa ggcgtcgatc acccgcgcgc cgaactcacg ctcgacgttg aggatgaaga
    16621 acgggatgtc gagcagctgc gccacccgac gcgcgtcatc cgcggcatcc ggcgagcagc
    16681 aggagcgcga cagttcatag ccctccggca ggtcgccgtg cgtgcgcatc cacacgccga
    16741 tgacctcgtc gtcaccgcgc tcgagagcca gcagcgccgc ggccacgctc gagtccacgc
    16801 cgccgctcat ggccgccacg attcgcgtca tgccgtggtc accgccttcg ccgtcgcctc
    16861 gcgcgccccg cgcatccgtc cgacgacgct cgcggtcacc tggagggtgc gctcgacgtc
    16921 cgcggccgtc gtctcgcgtc cgatggtcag gcgcagtgag ccgtgcgcca gctgggcgtc
    16981 gatgcccatg gccagcagca cgtgcgacgg ctcgacgctg cccgtcgtgc aggcgctgcc
    17041 cgtgctggcc tcgatcccct cgaggtccag cgccgcgacc aggtcaccgc cctcgatccc
    17101 gtcgatcacg accgaggcgc tgttcggcag ccgctccgtc gggtgtcccg tcagctgaac
    17161 gccaccgagc gcggtcagcc ccgccaccag ccgatcgcgg agcgcggcga cttcgaccac
    17221 cggcagcgag gccatcgtgg cggcggcgaa cccgacgatg cctgccacgt tctcggtccc
    17281 cgctcgccgc tgccgctcct gcgatccccc gttgacctgg ggcagcatgg cggtgccctg
    17341 gcggacgtac agcgcgccga cccccttcgg gcccccgagc ttgtgggccg acaggctcac
    17401 cagggccgcc tgccacgcgt cgggcgccgg cggcgcgaag acggccgact gcaccgcatc
    17461 gacgtggaac agcacgccgc gctcccggca cagcgcgccg atctcgacga tcggctgcac
    17521 ggtgccgacc tcgttgttgg cggcctgcac gctcaccagg gtggtgcggt ccgtgatggc
    17581 cgccgccacg tcggccgggt cgacccggcc gtatcggtcc accgggacga ccgtgacctc
    17641 gaagttctgc cgctcgagca ccgccgcggc gttcagcacg gccttgtgct ccaccggcga
    17701 tgtgacgatg tggcggccgc gcgcgctggc ggcccaggca acgcccttca gcgccaggtt
    17761 gtcggactcg gtcccgccgg acgtgaagac gatctcgcgc gcgcgggcac ccatccatgc
    17821 cgcgatccgc tcgcgcgctt cgtccaggcc ctggcgcgca cggcgccccg agccgtggat
    17881 gctcgaaggg ttgccgtgat gctcggtcag gtacgggagc atggcctcga gcacctcggg
    17941 tcgcagcggc gaggtggccg cgtgatcgag gtagatcgcc atggcgccga tgatagcggc
    18001 ggaccgatcc gtaggccgct tcccccgctg ggcgatccgc gttgtcgcct gtgcgatgct
    18061 cgcctgagga gcattgcgtc ggtcggctcc cacttccctg gtcggatggg tcctgcgagg
    18121 agccgttcgt ccggcgagag gcggtccagt gccccgactc ggttacccgt ccgacggaat
    18181 gcccgcccgg ggagcagggc ccgacgctaa ggttcggctg ccactcgcac cacaggttcc
    18241 cgcatgatca tccgtaccct cgaggagatc actggctcac cgaacgacgt cggcggggag
    18301 ggctggcgcg cccggcgcca cttcgtccgc gccgatggcc tgggcttctc gatgtcggag
    18361 accacggtcg aggcgggcgc cgagatgcag ctgtggtaca agcaccacaa cgaggcgtgt
    18421 tacgtgatcg aggggtcggc cgagatcacc gagcgcgaca cgggcgcggt gcatcgcatc
    18481 ggtcacggcg acgcgtacgc tccccagcac gatcgccaca ccatccgcgt cctcgaggcg
    18541 ctgcgactgg tctgcgtctt ctccccggcg ctgagcggcc acgagaccca cgacgccgat
    18601 gggagctacg agccctcccg agactgagcg cgttctgtgc tcgtggttga cccgtgggac
    18661 gggtcaacgg accctcgaac ggctccatgc ggacgctccg gtagcagatg acccgttgca
    18721 cgggtcaacg gaccccccat cggtccgatg ccgacactct ggtcgcggat gacccgcggg
    18781 acgggtcgcg cgagacagcc gccgagtcag ccgctgatct ccaggcgcac gcagacgttc
    18841 tctccggcgt tccccgtgca cagccgccac gagccgggct cggcgacgtc cgggatgacg
    18901 acccggtccg gccccggccc ggcgatcccc acgtcctcga ccgccacgtc gtcgcggtcc
    18961 gccgggtacc actgcggctc gccaccattg gcatccgaga tgagcaggta gcggtagacc
    19021 caggtcgagc ccacctcccg ctcgaggacg aacatgatcc cgcgcaccat ctggtcgggg
    19081 aacccgagct cgacgacgtc accgggcgcg gccgtctcgc ggtcggcgac catcgacgcc
    19141 gggcggaaga ccgccgtcgc atcgtgcggt gtgccggccg ggatcacgga gccgcagccg
    19201 gccagggtcg caccaacgag cagcgcggcg gccagccgcc gcatcggtca gtcctcctcg
    19261 acgacccgga ggtgcagctc gtccagctgc accggggcga ccggcgaagg ggcgcgcatc
    19321 atcaggtccg acccggactg cgtcttgggg aaggccatca cctcgcggat gttgtccgcg
    19381 ttggccatca gtgcagccca tcggtcgatc cccagcgcga tgccgccgtg gggcggcgcc
    19441 ccgtactcga gtgcgtccag caacgcgccg aactcgtcgc gcatgccctc gatcgagtgc
    19501 cccatcagcg cgaaggcccg ttcgagcagc ggccgctggt ggatccggac cgatccgccg
    19561 ccgagctccc agccgttgag ggccaggtcg tactgctgcg cgcgcaccga gccggggtcc
    19621 gattcgatcc gctccacgtc atcggggacg ggagcgctga acgggttgtg ggtggcgtcc
    19681 cagcgctggc cttcggggtc ccaggtgaac atcgggaagc ggtggaccca gacgtaggcg
    19741 aggaccgacg ggtcgcgcag gccgagccgc tcccccatct ccaggcgcag gcgacccagc
    19801 gcctcggccg cggcaatgcg gtccgcgtcg gcgacgatca gcagcagatc gccgggcgcg
    19861 ccgccgagcg cctcccgcac ggaagcttcg gcggagccga ggaacttggc cgcggggccg
    19921 tgcaacgcgc cgtcgggctg gaccgcgacg tggacgaggc cctttgcccc gccccgcttc
    19981 gccaggtcgg tgagctcgtc cagctgcttg cgcgtgtact cgccgcagcc cggcgcgcgg
    20041 atgccgagca ccgcgccgcc ggcggcgatc ggctcggcga acacccggaa gtcgacgccc
    20101 cgaacggcat cattgagctc gacgagctcc atgccgaagc ggatgtcggg cttgtcggag
    20161 ccgtagcggg ccatcgcctc gtcgtaggtc agccgcggga acggctcgac caggatcggg
    20221 cgctccggcg tcacctcccg ggacaccgcg atgaccgcgc gctcgaccgc ggccatcacg
    20281 tcggcctcct ggacgaagct catctccagg tcgagctgcg tgaactccgg ctggcggtcc
    20341 ccgcgcaggt cctcgtcgcg atagcagcgg gcgagctggt agtagcggtc gtagcccgca
    20401 accatgagca gctgcttgag ctgctgcgga gactgcggca gcgcgtagaa ctcgcccggc
    20461 tgcaggcgag acgggaccac gaagtcacgc gccccctccg gcgtcgagcg gatcagggtc
    20521 ggcgtctcga cgtcgacgaa gccgtcggcc tcgagcgtgc gacggatggc gcccgagagg
    20581 cgggcgcgca ggacgatgcg gtcctgcatc ggtggacggc gcaggtccag gtagcggtag
    20641 cgcaggcgca gcccctcgtc gagtccgggc tgctcctcgt tgatgtagaa cggcggcacc
    20701 ttcgacggat tgagcacggt gaaccgatcc accgccacct cgacctcgcc ggtcgccagc
    20761 tcggcgttgg tcgtgccgtc cggccgatgc cgcaccacgc cctcggcctg caccacccac
    20821 tcgttgcggg ccggctccgc ggccgcgtgc gcctccggcc cctcctgagc attcgtcacc
    20881 acctgggtga tgccgtggcg gtcgcgcagg tcgaagaagg cgaggtggcc gtggtcgcgg
    20941 cggcgatgga cccatccggc cagcgtcacg cgctggccga catcggccgc gcgcagctcg
    21001 ccgcaggtgt gcgttcggaa ggcggtgctc atgggagcca gcatcgtatc ggaaatccgt
    21061 cgcatggcat gcttcgcgac cgatgacgac gatgtcgcgt gccgagtgtc tccaggcgct
    21121 gccccgcgcc ctgcgctccc agcagtcgtt cggaccgctg tcgctgccgg tgacgctcgg
    21181 cctgctcgcc ggcttcgtcg cctccttcct ggccgatgcg ttccttttcg accagctgcg
    21241 caccccgtgg cgccaggcgg tcgacggcgt cgtcttcgcg gtggtcgcgg caccggtgtg
    21301 gatcggggtg cagcggcgcg acgtgcgatc cgcgatcgac gccctgacct ggctgaacgg
    21361 ctgggagacc gagcgctggc agcgcgagct cgggcggcgg ctcggcgcgg tcccgcgcgc
    21421 cacgccggag atcctggatc acctgcccga cacgatgggc ctgcgcccgc tgcggatcga
    21481 gctgctcggc gtgcgcggcg agctggacga ggcgtggcag cgactcgatg agcttccgac
    21541 ggacacgccc tggcagcggt tcgagcgcgc gtggctcaac gagtggctgc tctggctctc
    21601 cggtggaccg tcgcaggtga ccgagatgga ggcagcagcc ggcaccatcc cgacggacga
    21661 cgaccgggcg ctcgtcgcac gcacgatgct cgcggcgcag caatcgcggc gagcggcagt
    21721 gtcgggtggc gacgcggtcg caccactggc gagcgttcgg ccggcgctcg gcaaccgccc
    21781 cggccgctac acgctgggct atcggaccgg cgtgatcgtg tccgtgctga tggtgagcgt
    21841 cgtcgcggtc gcggcggtga tggtgacctc cgcgatcatt cgctgacgcg gttggcgccg
    21901 ccgacccgcg acgccacttc ggccaccggc accgtctcct gttccccggt ttccaggttc
    21961 ttcaggccga cccgccacgc cgccgtctcc tcgccgacga tgaccgccca cctcgcgccg
    22021 accttgccgg cggactccag ctgcttgccg agcttccgtg ccgagccatc cggacggacc
    22081 gacaggccag cgtcgcgcag cgcggccgcc acccgcaggc gatcgacgag atcgtccgcc
    22141 gacgttccga ccacggccac cagggcggtt ccggcctcga cggccacgcc ctggtcctcc
    22201 atggccagca cggtccggtc gacgccgacg ccgaacccga tcccggccgt gggacgtccg
    22261 ccgagcagct cggccaggcc gtcgtagcgg ccgccaccac ccagcgcctg ctgctggccc
    22321 tcgcgcccgg cgatgacgaa ctcgaacgtc gtgcgggtgt agtagtcgag cccgcggacc
    22381 agccggtgat cgaccacgtg gcgcaaaccc agggcgtcga tcaggtcgag caccgtccgg
    22441 aagtgggcgc ggcaggcgtc gcacaggtag tcggtcgact tcggcgcgcc cgcggcgagc
    22501 tcgtcgtcga gctgcttgtc gtccaggacg cgcagcggat tgacatccag gcggcggcga
    22561 gcgtcctcgc tcagcgcatc ggcatgctgg ctgaagtagt cgatcagcgc ctgccggtag
    22621 gcgggacggc acgccgcgtc accgatcgag ttcacgtgcg cgaccacgtc gtgcaggccg
    22681 acctccgcgt agaaccgatg cgccagctcg atcagctccg cgtcgacgac cggccccgga
    22741 tcaccgagca cttcgacgtc ccactgcgtg aactgccggt accgtccggc ctgcggccgg
    22801 tcgtagcgga acatcggccc gatcatggtc acgcgcagcg ggccgggacg gatgtgcagg
    22861 ccgtgctcga tgtaggcccg gacgatgccc gccgtcggct cggggcgcag cgcccactcg
    22921 gcccgctcct cgtcgctgcc cgtggcaccg ccgacccgga acatctcctt ctcgacggcg
    22981 tcgctggact ccccgaggcc gcgagcgaag agctcgacgc gctcgaagag cgggatctcg
    23041 agccgatcga atgcgtagcg ccgcgacaga tcccgcgcca cgccctcgac gtggctccac
    23101 gccgcggtgt gctcggggag caggtcgcgc gtgccacgcg gggcgctgat cgtcctggcc
    23161 atgcggtgga gtctatcggc cccgcaggtc gaccagccgg cgctcgcggc ggctcacctc
    23221 cgcggcgaac gccatgaggt ggctctcgag cgaggtcgcc agcgaggtcc ggccctcgcg
    23281 tgccgcgccc acctcgccgt cacgacgcgc ccgcaggcgc tcgatgacct cgtccagcat
    23341 ggcgtggtcg ccgccgccgt gcccgtccac cgttggcggc gcggcggtga gcgtgatcgt
    23401 ctcgtcgccg cgcagcgtgc tgcggccggc atcctcgcga tcccacgccc ccggaggcgt
    23461 ctcgccgccg ggaaggaagc ggcggatctc gatctcgccg gtgtcgaggc ggccgcgcag
    23521 ctcgccgcgc gtgccgttca gggtgatggt gcgcgtgttg tccgccgtga ggccgttcac
    23581 catcagcgtg gcccgcacgc cgttcgagaa ctccagggtc gccacctggt gatccgcgac
    23641 gtcgttgtcc gcgtggtaga cgcagcgccc gtacggcccg gtggccaccg cccgcagccg
    23701 gccctcacgc gagggctcct ccgccatcac cgatacgggc cacgccgt
//
LOCUS       DASTYP010000232         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1190860, whole genome shotgun sequence.
ACCESSION   DASTYP010000232 DASTYP010000000
VERSION     DASTYP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190860"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VFV59_10060"
     CDS             <1..1204
                     /locus_tag="VFV59_10060"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5326213.1"
                     /translation="GAYRAGKISLEELREIEDLSCPGPGACGGQFTANTMSTALEFLG
                     ISPGGLNAIPATDPAKLDAAYRSGELAMELVNGGIRPSSIMTRAAIDNAIASVAATGG
                     STNGVLHLLGIAHELGIPLELEDFDRVAARTPIVASLQPGGRFTALDIHEAGGVGLVA
                     RELLKRDLLDGSTRNVDGRTLAEVAAAAVETPGQEVVVSIDHPIKPTGGLAILRGSLA
                     PDGCVIKLAGHERRFHRGPARVFDSEAACYDAVRAQRIGPGDVVVIRYEGPVGGPGMQ
                     EMLSVTAALVGEGLGGDVALLTDGRFSGGTHGLMIGHVAPEAALGGPIALVDEGDEIV
                     IDVDARRLDLVVDEDELARRRAGWSPPEPRYAGGVLAKYAALVSSASDGAVTTGARMA
                     ANLRRHQG"
     gene            complement(1239..2036)
                     /locus_tag="VFV59_10065"
     CDS             complement(1239..2036)
                     /locus_tag="VFV59_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020108011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5326214.1"
                     /translation="MDPEYADVGGNRIAYRRVGHGPLIVLVHGAVCDGRVWSRQLLTL
                     ADLFTVVAWDAPGCGESSDPPESFRLPEYADALAGLIATLGGGEAHVVGHSFGGALAL
                     EVARRHPDVVASMTLVGAYAGWAGSLTSDEVQRRLSFALDVADRLPGGFDPTSMPGLF
                     TPAMDRAVADELAGIMSQIRPAATRAMAHALAEADLRGSLERIDVPVLLLYGDADKRS
                     PLHIAEYLRTHIPGARLVVLSGAGHYLHLEDPAPFEAALRQFLSDVA"
BASE COUNT          354 a          805 c          837 g          329 t
ORIGIN      
        1 cggcgcctat cgagcgggga agatctcgct cgaggagctg cgggagatcg aggatctgtc
       61 gtgcccgggc cctggcgcgt gcggcggcca gttcacggcc aacaccatga gcaccgcgct
      121 cgagttcctc ggcatctcgc caggcggcct gaacgccatc ccggccaccg acccggccaa
      181 gctcgacgcg gcgtatcggt ccggtgagct cgccatggag ctcgtcaacg gcggcatccg
      241 cccctcgtcg atcatgacgc gcgccgccat cgacaacgcg atcgcttcgg ttgccgcgac
      301 cggcggctcg acgaacggcg tgctgcacct gctcgggatc gcgcacgagc tggggatccc
      361 gctggagctc gaggacttcg accgcgtcgc cgcgcggacc ccgatcgtcg ccagcctcca
      421 gcccggcggc cgcttcaccg ccctggacat ccacgaggcc ggcggcgtcg gcctggtcgc
      481 ccgcgagctg ctgaagcgcg acctgctcga tggctcgacg cgaaatgtcg atgggcgcac
      541 gctcgcggag gtggccgccg cggccgtcga gacgccgggc caagaggtcg tcgtctcgat
      601 cgatcacccc atcaagccga ctggcggcct cgccatcctg cgtggatcgc tggcgccgga
      661 cgggtgcgtc atcaagctcg cgggccacga gcgccggttc catcgcggcc cggcgcgcgt
      721 cttcgactcg gaggcggcct gctacgacgc ggtgcgcgcg cagcgcatcg gtccgggcga
      781 cgtcgtcgtc atccgctacg agggccccgt cggcggcccc gggatgcagg agatgctctc
      841 ggtcaccgcc gcgctcgtgg gggagggcct ggggggcgat gtggcgctcc tcaccgacgg
      901 ccggttcagc ggcgggaccc acggcctgat gatcggtcac gtggcgccgg aggcggccct
      961 gggtggaccg atcgcgctgg tcgacgaggg cgacgagatc gtcatcgacg tggacgcgcg
     1021 gcggctggac ctggtggtgg atgaggacga gctcgcgcgg cggcgcgccg gatggtcacc
     1081 gccggagccg cgctacgccg gcggcgtgct ggccaagtac gccgcgctcg tgtcgtcggc
     1141 ctcggacggg gcggtcacca ccggggcgcg gatggcggcc aaccttcgcc ggcaccaggg
     1201 ctgattgcgg ctggtcggtg atcgagatcc gggcgaggtc aggcgacgtc gctcaggaac
     1261 tgacgcaggg ccgcctcgaa gggagccgga tcttcgaggt ggaggtaatg cccggccccc
     1321 gacagcacga cgagtctcgc gcccgggatg tgcgtgcgca gatactccgc gatgtggagc
     1381 ggggatcgct tgtcggcgtc gccgtacagc agcagcactg gaacgtcgat gcgctcgagc
     1441 gaaccgcgca ggtcagcttc cgcaagggcg tgcgccatcg ctcgcgtcgc ggccgggcgg
     1501 atctgcgaca tgatgcctgc gagctcatcc gccaccgcgc gatccatcgc cggcgtgaag
     1561 aggcccggca tggaggttgg atcgaatccg ccgggcagac ggtcggcgac atccagagcg
     1621 aagctcaggc gccgctggac ttcgtcggaa gtgagggagc ccgcccagcc cgcgtaggcg
     1681 ccgacaagcg tcatcgaggc cacgacatcg ggatggcgcc gggcaacctc cagcgccagg
     1741 gcaccaccga aggaatggcc gacgacgtgc gcctcgccgc ccccgagggt cgcgatgagg
     1801 cctgccagag cgtcggcgta ctcggggagc cgaaacgatt cgggaggatc cgacgactcg
     1861 ccacagcccg gtgcatccca agcaacgacc gtgaacaggt ctgccagcgt cagcagctgt
     1921 cgactccaca ccctcccgtc gcagaccgct ccgtggacga gaacgatgag cgggccgtga
     1981 cccacccgtc gatacgcgat gcgatttccg ccgacgtcgg cgtactccgg gtccatgtct
     2041 gggcccatgg ttgcacacgt cttccatacc ggtgacggca agggttgaca cggctgctag
     2101 ggtcgcccca tgtcagagga gcgcggcctc agcgccgcca gcccgaacgt gcgaccggcg
     2161 accgccggcg tgctcggcat gcttctcgta ctctcgattc ttcggctcgt cattctcctt
     2221 ggatggcggg atggaggacg cgccagcgga tagccgagag gcgaaccgaa cgcagcgaac
     2281 ccgagtcccg ccagccgaaa ggcccggcgg gatttctcgt ttctt
//
LOCUS       DASTYP010000233         5294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1190966, whole genome shotgun sequence.
ACCESSION   DASTYP010000233 DASTYP010000000
VERSION     DASTYP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190966"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFV59_10070"
     CDS             <1..789
                     /locus_tag="VFV59_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010638191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5326215.1"
                     /translation="VSFTGSTEVGRHVGSLAGGLLKKVSLELGGNNAFIVCDDADLDA
                     AASAGAYSSFQFQGQVCFAVGRHIVHRSVVDEYVSLLSERARRLRTGDPYREDVDLGP
                     IVNEQQLARVDGIVRRSVEGGARVAEGGTHEGLFYRPTVLADVTTDLPAWTDEIFGPV
                     APVVTFETDDEAIALANSSQYGLAGGVYSRSISRGLSIANRVRAGMVHVNDQTINDEA
                     IIPFGGMGLSGNGGRFGGEANYETFTEWQWITIRDEPKSFPHPY"
     gene            799..1902
                     /locus_tag="VFV59_10075"
     CDS             799..1902
                     /locus_tag="VFV59_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5326216.1"
                     /translation="MTSIAEVARRAGVSTATASRVLAASDYPVSAATRQRVLDAASAL
                     DYVPNALARGLLKRQVPVVGVIVHDITDPYFNEVVRGVEDAASAAGYLVITCSSDRDP
                     AQENSYVRLLRSMRAAAVIFAGSGIDDAALNEELGRHLVGIRSNGSAVVHLSPHAFGV
                     PEVGVDNVGAMVAMVAALVRLGHRRIGFLAGPARLYVARERLVGYRDALRAAQIGFDP
                     ALVVESGFDQRGGMTGVDALLEAAPDVTAVCCANDLLAIGALARLAERGIDVPGRLSV
                     SGVDDVSIAAMTSPALTTVRIPLRELGQRAFAVAVAQMSGTAPPPVVLPTEVVLRAST
                     GPPPELAMAGSDASRSVGAASASVSAEGAARAG"
     gene            complement(1871..3058)
                     /locus_tag="VFV59_10080"
     CDS             complement(1871..3058)
                     /locus_tag="VFV59_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5326217.1"
                     /translation="MQRPAELASALGPSGASGLSPARALAALFVAALALRPQLVGLGP
                     LLPAIQEDLGIPHGVAGLLTALPVLCMALFALPAVRIAHRIGLRMAMTVALAAVVVFG
                     ALRPIVPSYPVVLLATIGIGVGIGMGGALLPRATTLLVPSRVAIGTGLFSSGIQTGAT
                     VAAGTAVPLALIGDWRFALWVFAAAGALLLLAWLAATGPVADLTTDDVWPRFPWGSTV
                     AWRMVGIFALNSLVFYGLVTWLPSAYVDRGWSEVDAGRLVGIMNLAGIPVTLLIPWLS
                     DRSFPRHRTVLAAGVATVVALAGMLSAPELSLLWVPLAGASLGALFPLSMLLPNDIGP
                     HDIAGHAAMMLTGGYAVAAIAPALLGVARDTTGGFAVPFWILLLGGVVLCALSLRELR
                     PRH"
     gene            complement(3067..3924)
                     /locus_tag="VFV59_10085"
     CDS             complement(3067..3924)
                     /locus_tag="VFV59_10085"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU5326218.1"
                     /translation="MTQRVALLGKPLRRRHSVVMHNAAFDAAGIDARYELLEIEPEDV
                     PAAVEAARGDGWLGLGVTAPYKRVVAELVDEVEDAAAEIGAVNNVIRTDDGLLVGINT
                     DAPGFRAGVELAMGRPLDGARVVVAGAGGAAHAVVHACLTAGARHVTVANRTLASAVV
                     LVERFASVGGGTASAVALDDPVFRAALAEADVAVNATTVGMVDLGATIDVVALPPHAT
                     VFDLVYVPPETPVVAAARARGLRAANGGEMLIQQAAIAFGRWTGVAGMDGVMRRAVEP
                     LLLDPSLTA"
     gene            complement(3921..4967)
                     /locus_tag="VFV59_10090"
     CDS             complement(3921..4967)
                     /locus_tag="VFV59_10090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU5326219.1"
                     /translation="MADVGRFGVFLPSYIWEGDGPERARGIKTFARQVEDLGFDSLFI
                     TDHLLAAKRFYSVNWLEPLTTLAVAAGATERVRLGTSILIMPLRHPVILAKELATLQF
                     LSDNRVILGAGVGWNEAEYEAVGVKKSERGKRTDEMLDIMMPMLEGETVTYHGTYYSV
                     DEMYIEPRTEQRPLLWIGGGSQLADPKSPDVPRFVESVKQRTVKADGWIPRPTCPPQD
                     IARDWQELQEAVTEAGGDPKDMLVAHENFLHLVLTNDPAKAREEQHRAFLKVMSNERG
                     PQYLESVYLFGTPDEIIASLQARVDAGVEYFFLHTMTPDPAQLQHWVDEIIPNVNFPE
                     SAGPVRRPPRRWVR"
     gene            complement(4992..>5294)
                     /locus_tag="VFV59_10095"
     CDS             complement(4992..>5294)
                     /locus_tag="VFV59_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5326220.1"
                     /translation="LILTGRILDAEEAERIGLVSRVVPAGRAVAAAHEIGEEIAARGP
                     VAVREAKRLVALATDVDIAAGLAAELDASDRVFASEDMLEGARSFFEKREPKYRGR"
BASE COUNT          765 a         1944 c         1877 g          708 t
ORIGIN      
        1 gtctcgttca ccggctcgac cgaggtcggc cgacacgtcg gttcgctcgc cggcgggctc
       61 ctcaagaagg tgtcgctcga gctgggcggc aacaacgcct tcatcgtctg cgacgacgcc
      121 gatctggacg cggccgcatc ggccggtgcc tactcgtcgt tccagttcca gggccaggtc
      181 tgcttcgcgg tcggccgtca catcgtccac cgctcggtgg tggacgagta cgtctcgctg
      241 ctgtcggagc gggcgcggcg cctgcggacg ggcgacccgt accgcgaaga cgtcgatctc
      301 ggaccgatcg tgaacgagca gcagctggcc cgcgtcgacg ggatcgtgcg ccgctcggtc
      361 gagggcggtg cccgcgtggc ggagggcggc acgcacgaag ggctgttcta ccgcccgacg
      421 gtgctggcgg acgtcaccac cgacctgccg gcctggaccg acgagatctt cggcccggtc
      481 gccccggtcg tgaccttcga gaccgatgac gaggcgatcg ccctggccaa ctcgagccag
      541 tatgggctcg ccggcggcgt ctactcgcga tcgatctcca ggggcctgtc catcgccaac
      601 cgggtccgcg cgggcatggt tcacgtcaac gaccagacca tcaatgacga ggccatcatc
      661 ccgttcggcg gcatgggcct gtcgggcaac ggtggccggt tcggcggcga ggccaactac
      721 gagaccttca cggaatggca gtggatcacc atccgtgacg agcccaagtc cttcccgcat
      781 ccgtactgag cggcgacggt gacgtcgatc gccgaggtcg cgcggcgcgc cggggtctcc
      841 acggccacgg cgtcccgcgt cctggcggcg tccgactatc ccgtcagcgc cgcgacgcgg
      901 cagcgcgtgc tcgacgccgc ctcggcgctc gactacgtgc cgaacgccct cgcccgaggg
      961 ctgctgaagc ggcaggtccc ggtggtcggc gtcatcgtcc acgacatcac cgacccctac
     1021 ttcaacgagg tcgtgcgcgg cgtcgaggac gcggcctcgg ccgcgggcta cctggtcatc
     1081 acctgcagct cggaccggga tccggcgcag gagaactcgt acgtccggct gctccggtcc
     1141 atgcgcgcgg ccgccgtgat cttcgccggg agcggcatcg acgacgcggc cctgaacgag
     1201 gagctcggcc ggcacctggt cgggatccgg tccaacggct ccgcggtggt ccacctctcc
     1261 ccacacgcgt tcggcgtccc ggaggtcggc gtcgacaacg tcggcgccat ggtcgccatg
     1321 gtcgcggccc tcgtgcggct gggacaccgg cgcatcggct tcctcgccgg ccccgcccgg
     1381 ctgtacgtgg cgcgtgagcg cctggtcggt taccgcgacg ccctgcgcgc cgcccagatc
     1441 ggcttcgacc cggccctcgt ggtggagagc gggttcgacc agcggggcgg catgaccggc
     1501 gtggatgccc tcctggaggc ggcgcccgac gtcacggccg tgtgctgcgc caacgacctg
     1561 ctcgccatcg gcgcgctggc gcgcctggcg gaacgcggga tcgacgtgcc gggacgactg
     1621 tcggtcagcg gcgtggacga cgtgtcgatt gcggccatga cgtcgccggc gctcaccacg
     1681 gtccgcatcc cgctgcgcga gctcggccag cgtgccttcg ccgtcgccgt cgcccagatg
     1741 agcgggacgg cgccgccgcc cgtcgtgctg ccgacggagg tggtgctgcg ggcctcgacc
     1801 ggcccgccgc ccgagctcgc catggccggc tcggacgcct cgcgctcggt gggagccgcg
     1861 agcgcatcgg tcagtgccga gggcgcagct cgcgcaggct gagcgcgcac aggacgacgc
     1921 cgccgaggag caggatccag aacgggacgg cgaagccgcc ggtcgtgtcc cgcgcgacgc
     1981 ccagcaacgc cggcgcgatg gcagccaccg cgtacccacc ggtcagcatc atcgccgcgt
     2041 ggccggcgat gtcgtgcgga ccgatgtcgt tgggcagcag catcgacagc gggaagaggg
     2101 cccccaggga cgccccggcg agcggcaccc acagcagcga gagctcgggc gcgctgagca
     2161 tgccggccag agccacgacc gtggccacgc cggccgccag caccgtgcgg tgccgcggga
     2221 acgaccggtc cgacagccac gggatgagca gggtcaccgg gatcccggcc aggttcatga
     2281 tcccgaccag ccggcccgcg tccacctcgc tccagccccg gtcgacgtac gcgctcggca
     2341 gccaggtcac caggccgtag aagacgagcg agttcagggc gaagatcccg accatgcgcc
     2401 aggcgaccgt cgagccccac gggaagcgtg gccagacgtc gtcggtggtg aggtccgcga
     2461 ccgggccggt ggcggcgagc cacgccagca gcagcagcgc gccggcggcc gcgaagaccc
     2521 acagcgcgaa gcgccagtcg ccgatgaggg ccagtggcac cgccgttccg gcggcgacgg
     2581 ttgcgccagt ctggatcccg ctgctgaaca gtcccgtgcc gatggccacg cgggacggga
     2641 cgagcagcgt cgtggcccgt gggagcaacg ccccgcccat gccgatcccg accccgatgc
     2701 cgatcgtggc cagcaggacg accggatacg agggcacgat cggtcggagc gcgccgaaga
     2761 cgacgacggc ggcgagggcg accgtcatcg ccatgcgcaa gccgatgcgg tgcgcgatgc
     2821 ggaccgcggg cagggcgaac agcgccatgc acagcaccgg aagcgccgtc agcagccccg
     2881 cgacgccatg cgggatgccg agatcctcct ggatcgcggg cagcagcgga ccgagcccga
     2941 cgagctgcgg gcgcagggcg agggcggcga cgaagagcgc ggccagcgcg cgcgcgggcg
     3001 acagcccact ggctccggat ggcccgaggg ccgacgccag ctccgcagga cgctgcatcc
     3061 ccggcgtcag gcggtcaggg agggatcgag aaggagtggc tccacggcgc gccgcatgac
     3121 gccgtccatg ccggccacgc cggtccagcg cccgaaggcg atggccgcct gctggatgag
     3181 catctcgccg ccgttcgcgg cgcgaagccc gcgcgcccgc gccgctgcca ccaccggcgt
     3241 ctccggtggg acgtagacga ggtcgaacac cgtcgcgtgc ggcgggagtg cgacgacgtc
     3301 gatcgtcgcg cccaggtcca ccatcccgac ggtcgtggcg ttgaccgcca cgtcagcctc
     3361 ggccagcgcc gcccggaaca ccgggtcatc gagggccacc gcgctcgccg tccctccacc
     3421 gacgctggcg aaccgctcga ccaggaccac cgcggacgcg agcgtgcggt tggcgaccgt
     3481 gacgtgacga gccccggccg tcaggcaggc gtgcacgacg gcgtgcgcgg cgcccccggc
     3541 accggccacc accacgcggg caccgtcgag cggccggccc atcgccagct cgacgcccgc
     3601 ccggaagccc ggcgcatcgg tattgatgcc gacgaggagg ccgtcgtcgg tgcggatcac
     3661 gttgttcacc gccccgatct cggcggccgc gtcctcgacc tcgtcgacga gctcggcgac
     3721 gacgcgcttg tacggcgccg tcaccccgag ccccagccag ccgtcaccgc gcgcggcctc
     3781 gacggccgcg gggacgtcct cgggctcgat ctccagcagc tcgtaccgcg cgtcgatgcc
     3841 ggcggcgtcg aaggccgcgt tgtgcatcac gaccgagtga cgccgccgga ggggcttgcc
     3901 gaggagcgcg acccgctgcg tcaccggacc cagcggcgcg ggggacgacg aaccggcccg
     3961 gcgctctcgg ggaagttgac gttcgggatg atctcgtcga cccagtgctg gagctgcgcc
     4021 ggatcgggcg tcatggtgtg gaggaagaag tactccaccc cggcgtcgac gcgcgcctgg
     4081 agcgacgcga tgatctcgtc cggcgtcccg aacaggtaga ccgactcgag gtactgtggg
     4141 ccgcgctcgt tgctcatcac cttcaggaag gcgcggtgct gctcctctcg ggccttggcc
     4201 ggatcgttgg tcaggacgag gtgcaggaag ttctcgtgcg cgacgagcat gtccttcgga
     4261 tcaccacccg cctccgtgac cgcctcctgc agctcctgcc agtcccgggc gatgtcctgc
     4321 ggcggacagg ttggccgtgg gatccagccg tcggccttca ccgtgcgctg cttcaccgac
     4381 tccacgaagc gcgggacgtc gggcgacttc gggtcggcga gctgcgaccc gcccccgatc
     4441 cagagcagcg gccgctgctc ggtgcgcggc tcgatgtaca tctcgtcgac cgagtagtac
     4501 gtgccgtggt aggtgacggt ctccccctcc agcatcggca tcatgatgtc gagcatctcg
     4561 tcggtcctct tgccgcgctc gctcttcttg acgccgaccg cctcgtactc ggcctcgttc
     4621 caaccgacgc ccgcgcccag gatcacgcgg ttgtccgaga ggaactggag cgtcgccagc
     4681 tccttggcca ggatcaccgg gtggcgcagg ggcatgatca ggatcgaggt gcccaggcga
     4741 acccgctccg tggcgccggc cgccaccgcc agcgtggtca gcggctccag ccagttgacc
     4801 gagtagaacc gcttcgccgc cagcaggtgg tcggtgatga agaggctgtc gaagccgaga
     4861 tcctcgacct gtcgggcgaa cgtcttgatc ccccgagccc gttccggacc gtcgccctcc
     4921 cagatgtacg acggcaggaa caccccgaac cgcccgacgt cagccatgtg cttcctccag
     4981 ctagggcggc ctcagcggcc gcggtacttc ggctctcgtt tctcgaagaa ggatcgggcg
     5041 ccctccagca tgtcctcgct ggcgaagacc cgatccgatg cgtccagttc ggcggccaac
     5101 cccgccgcga tgtcgacgtc ggtcgccagg gcgaccagtc gcttcgcctc gcgcacggcg
     5161 accgggccgc gcgcggcgat ctcctccccg atctcgtggg cggcggcgac ggcccggccg
     5221 gccggcacca cgcggctcac gagcccgatc cgctcggcct cctccgcatc caggatgcga
     5281 ccggtgagga tcag
//
LOCUS       DASTYP010000234         5094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1627996, whole genome shotgun sequence.
ACCESSION   DASTYP010000234 DASTYP010000000
VERSION     DASTYP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627996"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFV59_10100"
     CDS             complement(<1..353)
                     /locus_tag="VFV59_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEU5326221.1"
                     /translation="MTDAIRGAIESASAYLSEHPEEARYTDSVARATVVDGLRVRVEG
                     AAGEVLETDMPAGVGGRGEAPSPGWYFRAALAACVTSLATMRAAQLGWDGFRCSVEVD
                     SESDDRGILGLDRSVP"
     gene            complement(350..2428)
                     /gene="ligA"
                     /locus_tag="VFV59_10105"
     CDS             complement(350..2428)
                     /gene="ligA"
                     /locus_tag="VFV59_10105"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5326222.1"
                     /translation="MAQTDPAERAADLRARLNDANYRYHVLDAPTISDREYDLLFREL
                     VDLEAANPELIAPDSPTQRVGAPVEGGFPAVRHQVPMLSLGNAFGADELREFDARVRR
                     GLGRAESDAPVRYVCELKIDGLAISLRYEGRRFVRGATRGDGTTGEDVTPNLRTIRAI
                     PITLREDPPGDTLEVRGEVYMPRGAFAELNRQLEAEGKPLYANARNTTAGTVRQKDPR
                     VTASRRLSLWTYQLVGAEGLDSHSESLELLQRLGFPVNPHVRVVEGIDAVVAYTEDWA
                     TARRELDYETDGVVVKVDALADQRTLGFVSRAPRWAIAYKFPAEQVTTTLEEIDVYVG
                     RTGALTPVAHVTPVFVGGTTVRNATLHNIDEIRRKDLRVGDTVVLQRAGDVIPEVVSA
                     VVDARTGDETVWEMPTSCPACGTEVVREEGEAVTRCPNPYCPAQRIGGLLHFAGRGSM
                     DVDGLGERIMAQLIERDLVREPADIFRLDAETLEGLERLGRKSAENLAASIEGARHRP
                     LSRILNGLGIRHVGEQTAIDLAAWLGRELPRVDDETDAAWTRRAADRLRDASVEELTE
                     VYGVGRVVAEAIERFFDDEHTRDLLHHLVDAGVAAEAPAPGASAEPTEGPLSGKTLVV
                     TGTLPGFSRQEAEEAIRAAGGHAASSVSAKTDYLVAGDKAGSKLAKAEQLRVPVLDED
                     GFRRLLEGNS"
     gene            complement(2468..2968)
                     /locus_tag="VFV59_10110"
     CDS             complement(2468..2968)
                     /locus_tag="VFV59_10110"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5326223.1"
                     /translation="MPEVRIPALLAELDAARDAFSEALSGADAELLVAPGLVGDWSAR
                     ELIAHLAHWDRWAATCLEHPSTDGLGSLMTGEWDVDAQNAEVAARAAGMSLDRVRDDE
                     AEAFAAFRERLAALDGSALDVEAPWGGTLETIVRENGPDHYRPHAEEVAAWFAVDDEA
                     PDDDVE"
     gene            complement(2961..3437)
                     /locus_tag="VFV59_10115"
     CDS             complement(2961..3437)
                     /locus_tag="VFV59_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5326224.1"
                     /translation="MRIDELTAELTAERATFGDVVAGIAGASLTTPGLIGQWSAREVI
                     AHLGYWAGRATEAIHEVELGLAAEEDEVEEAGVDAINETVARVARETDVATVRRREEA
                     SYEALLERLRTLDPALLSTVLPDGSTLEETIREDGALHYREHADELRAALAGGAHA"
     gene            complement(3448..>5094)
                     /locus_tag="VFV59_10120"
     CDS             complement(3448..>5094)
                     /locus_tag="VFV59_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5326225.1"
                     /translation="DFEADYPDAKVVKLEQNYRSTQTILDAAHAVVSRNVGRKDKKLW
                     TDRGGGTQITLFDAYNEYEEAEFVARQIEKLVGAGRRGGMAALLTSRAGDEDGTLKYG
                     DVAVTYRINAQSRVLEESLMRFGIPYQLVGGTRFYERREVKDALAYVRLARNPADRVA
                     LLRIINVPARGIGAKTVEELNAWSESRDVSVWEAVQAAGENPNLAPRARAQLATFAEL
                     VRGLIGMAGAEPPSAIFDAALERSGLQSAIQDGSDEGEERWANVVELRNHAAEFDEIA
                     PPEGLARFLEEVALVSDQDELEDAPDRVTLITLHAAKGLEFPVVFIVGMEEGLLPHRR
                     ALEDERELEEERRLAYVGMTRAKDRLYLVHAHHRSTYGVGAQSDPSRFLAELPEDLLA
                     AERSGSPFRRGGDRWAGTDDEGGWLPGGYRSPTRRVREPLRPVSLPDLGAPSPPPPPR
                     PMGRQLDAARERVAAARYEAGTGVDLGSGTFSAAGAGDALGAEEEPSWKAGDRVKHRR
                     FGDGIVVSSRIVKGDEEVTVAFVGEGVKRLIAAYAGLERA"
BASE COUNT          654 a         1942 c         1738 g          760 t
ORIGIN      
        1 ggcacggagc ggtcgagccc caggatcccg cggtcatcgg actcgctgtc gacctccacg
       61 ctgcagcgga agccgtccca gccgagctga gcggcacgca tggtggcgag cgacgtcaca
      121 caggctgcca gcgcggcgcg gaagtaccag ccggggctcg gcgcctcgcc gcgcccaccc
      181 accccggccg gcatatcggt ctcgagcacc tccccggctg cgccctccac gcgcactcgc
      241 agtccgtcca ccacggtcgc gcgagcgacc gaatcggtgt agcgagcctc ctcgggatgc
      301 tcgctcaggt aggcgctcgc gctctcgatc gcgccgcgga tcgcatcggt catgaattgc
      361 cctccagcag tctgcgaaac ccatcctcgt ccagcaccgg cacccggagc tgctccgcct
      421 tcgccagctt gctgccggcc ttgtcaccgg ccaccaggta gtccgtcttg gctgagaccg
      481 atgacgccgc gtgccccccg gccgcccgga tggcctcctc cgcctcctgg cggctgaacc
      541 ccgggagcgt gccggtcacc accagggtct tgccgctcaa cggtccctcc gtcggttcag
      601 ccgatgcgcc gggcgccggc gcctcggcgg ccacgccggc gtccaccagg tggtgcagga
      661 gatcccgggt gtgctcgtcg tcgaagaagc gttcgatcgc ctccgccacc acgcgtccca
      721 cgccgtacac ctcggtcagc tcctcgaccg atgcgtcgcg cagccggtcg gccgcgcgcc
      781 gggtccaggc ggcatccgtc tcgtcgtcga ctcgcggcag ctcacgcccg agccacgccg
      841 ccaggtcgat ggccgtctgc tcgccgacgt gccggatgcc gaggccgttg aggatccggc
      901 tcagcgggcg gtgtcgtgcg ccctcgatgg aggcggccag gttctccgcg gacttgcggc
      961 ccagacgctc caggccctcc agcgtctcgg catccaggcg gaagatgtcg gccggctccc
     1021 gcaccaggtc gcgctcgatg agctgggcca tgatccgctc gccgagcccg tcgacgtcca
     1081 tgctcccgcg acccgcgaag tgcagcagcc cgccgatgcg ctgcgccggg cagtacgggt
     1141 tcgggcaacg cgtgaccgcc tcgccctcct ctcgcaccac ctcggtgccg caggccgggc
     1201 agctcgtcgg catctcccac accgtctcat cgcccgtccg tgcgtcgacc acggcgctga
     1261 cgacctccgg gatcacgtcc ccggcccgct ggagcaccac cgtgtccccc acgcgcaggt
     1321 ccttgcgccg gatctcgtcg atgttgtgca gcgtcgcgtt ccgcaccgtg gtgccgccga
     1381 cgaacaccgg cgtcacgtgt gccacggggg tgagcgcgcc ggtgcgaccg acgtagacgt
     1441 cgatctcctc gagcgtggtc gtcacctgct ccgcggggaa cttgtaggcg atggcccagc
     1501 gcggcgcgcg agagacgaag ccgagcgtcc gctggtcggc gagcgcgtcg accttcacga
     1561 ccaccccatc ggtctcgtag tccagctcgc gtcgggcggt cgcccagtcc tcggtgtacg
     1621 cgaccaccgc gtcgatgccc tcgaccacgc ggacgtgggg gttcaccggg aagccgagcc
     1681 gctggagcag ctccagtgac tcggagtggc tatcgaggcc ctccgcgccc acgagctggt
     1741 aggtccacag cgagagccga cgactggcgg tcacgcgggg gtccttctgc cgcaccgtcc
     1801 ccgccgtcgt gttccgggcg ttggcgtaga gcggcttccc ctcagcctcc agctggcggt
     1861 tgagctccgc gaaggcgccg cgcggcatgt acacctcgcc gcgaacctcg agcgtgtccc
     1921 ccggcggatc ctcgcgcagc gtgatcggga tggcgcggat ggtccgcagg ttcggcgtga
     1981 catcctcgcc cgtcgtcccg tcaccgcggg tcgccccgcg cacgaagcgg cggccctcgt
     2041 agcgaagact gatggcgagg ccatcgatct tgagctcgca gacgtaccgg acgggggcgt
     2101 cgctctcagc ccgaccgagg ccccggcgca cgcgtgcgtc gaactcgcgg agctcgtccg
     2161 cgccgaaggc gttgccaagc gagagcatcg gcacctggtg gcggacggcc gggaaaccgc
     2221 cctcgaccgg cgcgccgacc cgctgcgtgg gcgagtccgg ggcgatcagc tcggggttcg
     2281 cggcctccag gtccacgagc tcgcggaaca gcaggtcgta ctcgcggtcg ctgatcgtcg
     2341 gcgcgtcgag gacgtggtag cggtagttgg cgtcgttgag ccgagcgcgc aggtcggcgg
     2401 cgcgctccgc gggatcggtc tgggccatgg accgcgattc tagggtgcgg ccgccgggcc
     2461 gcggccgtta ctcgacgtcg tcgtcaggcg cctcgtcgtc caccgcgaac catgccgcca
     2521 cctcctcggc gtgcggccgg tagtgatcag ggccgttctc gcgcacgatg gtctccagcg
     2581 tcccgcccca cggcgcctcc acgtcgagcg cagagccatc cagcgccgcc agccgctcgc
     2641 ggaacgcggc gaaggcctcc gcctcgtcgt cgcgcacccg gtccagcgac atgccggccg
     2701 cgcgcgccgc cacctccgcg ttctgcgcgt cgacgtccca ctctcccgtc atcagcgagc
     2761 cgagcccgtc ggtcgagggg tgctccaggc aggtcgccgc ccatcggtcc cagtgcgcga
     2821 ggtgggcgat cagctcgcga gcgctccagt cgccgaccag gccgggcgcc accagcagct
     2881 ccgcatcggc tccggaaagg gcctccgaga aggcgtcacg tgcggcgtcg agctcggcga
     2941 ggagcgcggg gatgcgtacc tcaggcatgg gcgccacccg ccagcgcggc ccggagctcg
     3001 tccgcgtgct cgcggtagtg cagggcgccg tcctcgcgga tcgtctcctc cagcgtcgag
     3061 ccgtcgggca gcacggtgga cagcagcgcg ggatccaggg tgcgcagccg ctcgaggagc
     3121 gcctcgtacg acgcctcctc gcgtcgtcgg acggtggcga cgtccgtctc gcgcgcgacg
     3181 cgcgccaccg tctcgttgat cgcatccacg ccggcttcct cgacctcgtc ctcctcggcc
     3241 gccagcccca gctcgacctc gtggatggcc tcggtcgcgc gtcccgccca gtagcccagg
     3301 tgcgcgatca cttcccgcgc gctccactgc ccgatcaggc ccggtgtcgt cagcgaggct
     3361 ccagcgatgc cggcgacgac gtccccgaac gtggcccgtt ccgccgtgag ctcggcggtc
     3421 agctcgtcga tgcgcatcgg ttgctcctca ggcccgttcc agcccggcgt aggcggcgat
     3481 caatcgcttc acgccctcgc cgacgaaggc gacggtcacc tcctcgtcgc ccttgacgat
     3541 tcgactgctg accacgatcc cgtcgccgaa gcgccgatgc ttgacgcggt cgccggcctt
     3601 ccagctcggc tcctcctcgg cccccagcgc gtcccccgca ccggccgccg agaacgtgcc
     3661 ggagcccagg tccacgccgg tcccggcctc gtaccgggcg gccgcaacgc gttcgcgggc
     3721 ggcgtccagc tgccggccca tcggccgggg tggcggaggt ggcgagggcg cgccgagatc
     3781 cgggagcgag acgggccgaa gcggctcgcg gacgcgacgg gttggcgatc ggtagccacc
     3841 cggcagccag ccgccttcgt catcggtgcc cgcccagcgg tcgccgccac gccggaaggg
     3901 gctgccggag cgctccgcgg ccagcaggtc ctcgggcagc tccgccagga agcgggacgg
     3961 atcggactgg gcgcccaccc cgtaggtcga ccggtggtgc gcgtgcacca ggtagagccg
     4021 gtccttggcg cgcgtcatcc cgacgtacgc gaggcgtcgt tcctcttcca gctcgcgctc
     4081 gtcctcgagc gcgcgacggt gcggcagcag tccttcctcc atgcccacga tgaacaccac
     4141 ggggaactcg aggcccttcg ccgcgtggag ggtgatgagc gtgacgcggt cgggggcgtc
     4201 ctccagctcg tcctggtcac tgaccagcgc tacctcttcc aggaagcggg cgaggccttc
     4261 cggcggcgcg atctcgtcga actccgccgc gtggttgcgc agctcgacga cgttggccca
     4321 gcgctcctcg ccttcgtcgc tgccgtcctg gatggccgac tgcaggccgg atcgctcgag
     4381 cgcggcgtcg aagatggccg acggcggctc cgcgcccgcc atgccgatca acccgcgcac
     4441 cagctccgcg aaggtggcca gctgggcgcg cgcgcgaggc gccaggttcg ggttctcgcc
     4501 ggcggcctgc acggcctccc agacgctgac gtcccgtgat tccgaccagg cgttgagctc
     4561 ctcgaccgtc ttagcgccga tcccgcgcgc cggcacgttg atgatccgca gcagggccac
     4621 acggtcggcc gggttgcggg ccaggcggac gtaggcgagc gcgtccttca cctcccgccg
     4681 ctcgtagaag cgggtaccgc cgaccagctg gtacgggatg ccgaaccgca tcagcgactc
     4741 ctcgagcacg cggctctgcg cgttgatgcg gtacgtgacg gcgacgtccc cgtacttgag
     4801 cgtcccgtcc tcgtcaccag cccgcgaggt cagcagcgcg gccattccgc cgcgacgacc
     4861 ggcgcccacg agcttctcga tctggcgcgc gacgaactcg gcctcctcgt actcgttgta
     4921 cgcgtcgaag agggtgatct gcgtgccacc gccccgatcg gtccacagct tcttgtcctt
     4981 gcgtccgacg ttgcggctca cgaccgcgtg ggcggcgtcc aggatcgtct gggtcgagcg
     5041 gtagttctgc tcgagcttca cgaccttcgc atcggggtag tcggcctcga agtc
//
LOCUS       DASTYP010000235         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1628232, whole genome shotgun sequence.
ACCESSION   DASTYP010000235 DASTYP010000000
VERSION     DASTYP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628232"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..489)
                     /locus_tag="VFV59_10125"
     CDS             complement(73..489)
                     /locus_tag="VFV59_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5326226.1"
                     /translation="METDRPMILAKKPTRLSLLAGALVLVLAACGTTAADDQGSTVDP
                     ADADATVTAADMAFDPGTVTVAGGGSFTLAFVNEDAMPHNIAIYTDSSKTEKLFEGEV
                     VTDGTTVYEIPALDAGEYFFDCSLHPNMTGTLVVEG"
     gene            complement(563..994)
                     /locus_tag="VFV59_10130"
     CDS             complement(563..994)
                     /locus_tag="VFV59_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326227.1"
                     /translation="MSNFLRTRAAGFVVTAAIVGLTGATAYIHLGLGGLLFTLNGLGY
                     LGLAGLIVIGAAAPFAIVERFSWAPRLALMGYTLATIGGYLVMGPYFTLGFIAKGIEV
                     VLITFLVVDVIRVYGSPMGMVRAALTSVAPILPARFRSLAA"
     gene            1223..>4044
                     /locus_tag="VFV59_10135"
     CDS             1223..>4044
                     /locus_tag="VFV59_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5326228.1"
                     /translation="MTRAPARPTSAATTLTPVSPDDAETHALLARAVEEAARLLRADG
                     AMVYLVDSDGERLRFAVDAGIRDGVARQLIRDLRLPLGTGLFGHAVTTRELVTTPDYR
                     EDTRFPHSPVADRIVETAEMRSMAAAPLIAGDEVLGALGAFSSRSGGFSDAELALLRA
                     LSDHAASSIANQRLIARLARSQEELAHRVEAQRTLAGITAAIASIRDPNVVLQEVVDA
                     ARRLLGSDGAHIALMESGGHELRPSVMAGGTDERLRRWFSRLRFPVGGGINGLAARDG
                     CTVATEDYLVDPRIPHEDEDQRVAKRMGLRGMAAAPMRAPGGEVIGTLAVSFEQPHTF
                     SEDDLELLGGLSDQGAIAIANARLVESLSDSEARFRHLVTSSPDLVWETDATGRFTFL
                     SPRLLELTGWEPRELVGQPWSALVEDESMATARKTWEALQRDPDAVEKSRFLLRRKDG
                     DPIPAEVYAIGSQRDGTFVGAHGSIRDLSDAERLTEQLRRQADELADRVDAQRTLAEM
                     AAQLTTLRDPADALKQTLKAAVRLLKGHGGQIGMITTDPDGTLRWGDGHSLVRDQLTP
                     FTKDDRTPVDEGVSGRAVRERRASWTDDYLSDESFPHEPNSDRIARRLSIRSVMAAPL
                     IADDQPIGAIGVYAETVGAFSAESAELLRLLADQAAIVLTNARLYAEAEVTQAQLAHR
                     VEAQRTLGEIAASITSLRDPSAVLSRTVDEARRLLNAENVVIHQVRTGTRELADYREV
                     FNAGPDAKPVDEITVSIGQGIAGRAVAQQQVSWTGDYLADESFQHTAKADAWIRRNGY
                     RSQMSAPLIGEDGALGAISAYSARPGAFTDDDAQLLGALASQAAIVLGNARLYEELER
                     RVEAQRSLGEIATRITALRDPGDVLQRTLDEAVRLLDADGGRIELVAEGGGLHWAYGH
                     SAIDLPIERDEAIDPVQADEGVSGRAV"
BASE COUNT          608 a         1445 c         1470 g          521 t
ORIGIN      
        1 acgagagagc gccccgtccc ggaccggacg gggcgcccat ggtgacgcga ccaggggtcg
       61 gggagggtcg ggtcagccct ccacgaccag ggtgccggtc atgttcgggt ggagcgagca
      121 gtcgaagaag tactcgccgg catcgagggc cgggatctcg tagacggtcg ttccatcggt
      181 gacgacctcg ccctcgaaga gcttctcggt cttcgaggag tcggtgtaga tggcgatgtt
      241 gtgcggcatc gcgtcctcgt tgacgaaggc gagggtgaac gagccgccac cggccaccgt
      301 gacggtgccc gggtcgaagg ccatgtcggc agcggtgacc gtcgcgtcgg catcggcggg
      361 gtcgaccgtc gagccctgat catcggccgc ggtggtccca caggcagcca ggacgaggac
      421 gagcgcaccg gcgaggagcg agagccgcgt gggcttcttg gcgaggatca tcggtctatc
      481 ggtctccatg gttcgcatgc tgaaggcggg ccgagggccc ggcccgccca gcgttcgaag
      541 gtgtcggtcg gattggcggt ggctaggcgg ccagcgagcg gaagcgggcc ggcaggatcg
      601 gggcgacgga ggtcagcgcg gcgcggacca tgcccatggg gctgccgtag acgcggatca
      661 cgtcgacgac caggaaggtg atgagcacga cctcgatgcc cttggcgatg aagccgagcg
      721 tgaagtaggg gcccatgacc aggtagccgc cgatggtcgc gagcgtgtag cccatcagcg
      781 ccaggcgcgg agcccagctg aagcgctcca cgatggcgaa cggcgccgcg gcaccgatca
      841 cgatcaggcc ggccaaaccg aggtagccga ggccgttgag ggtgaagagc aggccgccga
      901 ggccgaggtg gatgtacgcc gtggcaccgg tcaggccgac gatggcggcg gtgacgacga
      961 agccggcggc gcgagtgcgc aggaagttgg acaccgtggg ttgcctcctg atgactgggg
     1021 tcgacgccgt gtccggcatc ggctgtcgtt gattccgatg cgccgaagtc tcgacgccgc
     1081 cagcccgtac cgaatccgcc ggatgcggga cctgcgcccc cgacatgcgc gccgcgagaa
     1141 gctcggtcgg atgacgtaga cggccccggc cgtacgccgc atgcggtcgc gcgcgcgtga
     1201 tggcgtatgc tccgcagcgc cgatgacccg agcccccgcg cgcccgacgt ccgccgccac
     1261 gaccctgacg cccgtctcac cggatgacgc cgagacgcat gccctcctcg ctcgcgccgt
     1321 cgaggaggcc gcccgcctgc tgcgcgccga cggggcgatg gtctacctgg tcgacagcga
     1381 cggcgagcgc ctgcgctttg ccgtcgacgc cggcatccgc gacggcgtcg cgcgccagct
     1441 gatccgcgat ctgcggctgc cgctcgggac cggcctcttc ggccacgccg tcacgacgcg
     1501 tgagctcgtc accacccccg actaccgcga ggacacgcgc ttcccgcact cgccggtggc
     1561 cgaccggatc gtggagacgg ccgagatgcg ctcgatggca gccgcaccgc tcatcgccgg
     1621 cgacgaggtg ctgggcgccc tgggcgcctt ctcgtcgagg tccggcggct tcagcgacgc
     1681 cgagctggcc ctcctgcgcg ccctctcgga ccacgccgcc tcgagcatcg ccaaccagcg
     1741 actcatcgcg cggctggcgc gctcgcagga ggagctggcc caccgggtcg aggcccagcg
     1801 cacgctggcc ggcatcaccg ccgcgatcgc cagcatccgc gatcccaacg tggtcctgca
     1861 ggaggtggtg gacgcggcgc gacggctgct gggctcggac ggcgcacaca tcgcgctgat
     1921 ggagagcggc gggcacgagc tgcgacccag cgtcatggcc ggcgggaccg acgagcggct
     1981 gcgtcgctgg ttcagccggt tgcggttccc ggtcggcggc gggatcaacg ggctcgcggc
     2041 ccgcgacggc tgcaccgtcg ccaccgagga ctacctggtc gatccgcgga tcccgcacga
     2101 ggacgaggac cagcgcgtcg cgaagcgcat ggggctgcgc ggcatggcgg ccgcgccgat
     2161 gcgtgcgccg ggcggcgagg tgatcggcac cctggccgtc tcgttcgagc agccccacac
     2221 gttctccgag gacgacctcg agctgctggg cggcctgtcc gaccagggcg ccatcgccat
     2281 cgccaacgcc cgcctggtgg agagcctgtc cgactccgag gcacggttcc ggcacctggt
     2341 gacctcgtca ccggacctgg tgtgggagac cgatgcgacc ggccgcttca cgttcctctc
     2401 cccgcgcctg ctcgagctga ccggctggga gccgagggag ctggtggggc agccgtggtc
     2461 ggccctcgtc gaggacgagt cgatggcgac cgcacgcaag acgtgggagg cgctccagcg
     2521 ggaccccgac gcggtcgaga agagccgctt cctgctgcgc cgcaaggacg gcgacccgat
     2581 cccggccgag gtctatgcca tcgggagcca gcgggacggc actttcgtcg gcgcccacgg
     2641 ctccatccgc gacctgagcg acgccgagcg cctcacggag cagctgcggc gccaggccga
     2701 cgagctggcc gaccgcgtgg acgcgcagcg cacgctggcc gagatggccg cccagctgac
     2761 cacgctgcgt gacccggccg acgccctgaa gcagaccctc aaggccgcgg tgcgcctcct
     2821 caaggggcac ggcggccaga tcggcatgat caccaccgat ccagacggaa cgctgcgctg
     2881 gggcgatggc cactccctgg ttcgggacca gctcacgccg ttcaccaagg acgatcggac
     2941 accggtcgac gaaggcgtct cgggacgtgc ggtccgcgag cggcgggcct cctggaccga
     3001 cgactacctg tcggacgaga gcttccccca cgagccgaac tccgaccgca tcgcgcgccg
     3061 cctctcgatc cgctcggtga tggcggcgcc gctgatcgcc gacgaccagc ccatcggcgc
     3121 catcggcgtc tacgccgaga cggtcggcgc cttcagcgcc gagtcggccg agctgctgcg
     3181 gctgctggcc gaccaggcgg ccatcgtgct gaccaacgct cgcctctacg ccgaggccga
     3241 ggtgacgcag gcccagctgg cgcaccgggt cgaggcccag cgcaccctcg gcgagatcgc
     3301 cgcctccatc acctcgctgc gcgatccgtc ggccgtcctc agccggaccg tggacgaggc
     3361 gcgccgcctc ctgaacgccg agaacgtggt gatccaccag gtgcgcaccg gcacgcgcga
     3421 gctggcagat taccgcgagg tgttcaacgc cggtcccgac gccaagccgg tcgacgaaat
     3481 cacggtcagc atcggccagg gcatcgccgg gcgcgccgtc gcgcagcagc aggtctcgtg
     3541 gaccggcgac tacctcgcgg acgagagctt ccagcacacc gccaaggccg atgcgtggat
     3601 ccgccgcaac ggctaccgct cgcagatgag cgctccgctg atcggcgagg atggcgccct
     3661 cggcgccatc agcgcctact ccgcccgccc cggggccttc accgacgacg acgcccagct
     3721 gctcggcgcg ctggccagcc aggccgccat cgtgctgggc aacgctcgcc tgtacgagga
     3781 gctcgagcgt cgggtcgagg cgcagcgctc cctcggcgag atcgcgacgc gcatcacggc
     3841 gctgcgcgat cccggcgacg tcctgcagcg cacgctggac gaggccgtcc ggctgctgga
     3901 cgccgacggt ggacggatcg agctggtggc cgagggcggc gggctgcact gggcgtacgg
     3961 ccactccgcc atcgacctgc cgatcgagcg cgacgaagcg atcgacccgg tccaggccga
     4021 cgagggcgtc tccggtcgcg cggt
//
LOCUS       DASTYP010000236         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1447524, whole genome shotgun sequence.
ACCESSION   DASTYP010000236 DASTYP010000000
VERSION     DASTYP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447524"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFV59_10140"
     CDS             <1..367
                     /locus_tag="VFV59_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326229.1"
                     /translation="FHDFDARMKEIVTGWQVREIDGEQALNDHTDPAYDAEILDQLAR
                     LHDELAAWMQPLADAFHRYAAYRARLGSSLERARAGDQRFVASPRVDSYHSVWFELHE
                     DLIRLAGRKRADEAAAGRA"
     gene            complement(369..1157)
                     /locus_tag="VFV59_10145"
     CDS             complement(369..1157)
                     /locus_tag="VFV59_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5326230.1"
                     /translation="MSRFDGRAVLITGSTGMAASAARAIAAEGGRVFVASRTADHARA
                     LGDELGGRHGWFAAELSQEDGAEAAVAAARDRLGRLDAVYAVAGISGRRFGDGPLHEA
                     TLEGWETVIGSNLTSLFLVSRAAVRAMLEQEPDADSQRGTILAMSSGLSRHPSAEYFA
                     THAYAASKGAIEAFVRATAAFYAPRGIRVNAIAPALVSTPMSRRAQDDPAILAYLRGK
                     QPLASGPIDPDSVTAVALHLLSRDARMVTGQVVDVDAGWSVSEG"
     gene            complement(1154..1795)
                     /locus_tag="VFV59_10150"
     CDS             complement(1154..1795)
                     /locus_tag="VFV59_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU5326231.1"
                     /translation="MSEALRSGDVADAVRRHRLIAVLRQVQPRSTLLALVEELADAGV
                     RVFEITLDAPDGADDLVAVRALLASRQSEPSIVGAGTILTSTQLEAAVAAGADFGVAP
                     VLDPQLLHAARASGLPFVPGAMTPTEIRTAWEAGAAFVKLFPASAVGPAMVRELRGPL
                     PDVELIPTGGIDATNAAAYLAAGAVAVGIGSSLTRADAAGRRALIDAVREAPR"
     gene            complement(1792..>2362)
                     /locus_tag="VFV59_10155"
     CDS             complement(1792..>2362)
                     /locus_tag="VFV59_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5326232.1"
                     /translation="DATDPEQLTRAEIDGRRQVREYARFLRDRVPGFESSVVVATSPA
                     IGVRESRRVHGDYVLTRDDVLYAARFDDEIALCGAPIEDHHAGGDTEWQYVPGGEVYG
                     IPYRALLPAGLDGLLVAGRCFSASHDAHASARSMATCMAMGQAAGTAAALAGRARCSP
                     REVHGGALRSRLADDGALLEPLAPAEVAP"
BASE COUNT          336 a          858 c          885 g          283 t
ORIGIN      
        1 gttccacgac ttcgacgcgc ggatgaagga gatcgtgacg gggtggcagg tgcgggagat
       61 cgacggggag caggcgctca acgaccacac cgatccggcg tacgacgccg agatcctcga
      121 ccagctcgcg cggctgcacg acgagctcgc ggcgtggatg caaccattgg cggacgcgtt
      181 ccaccggtac gcggcctacc gagcgcgcct gggatcgtcg ctcgagcggg cacgtgccgg
      241 ggatcagcga ttcgtcgcct caccgcgcgt cgacagctac cactccgtct ggttcgagct
      301 gcacgaggac ctcatccggc tcgcgggccg gaagcgcgcg gacgaggcgg cggccggtcg
      361 ggcgtgactc agccctcgct gacgctccaa ccggcgtcga cgtcgaccac ctggccggtg
      421 accatgcggg cgtcccgact gagcaggtgg agggcgacgg cagtcaccga atccgggtcg
      481 atcgggccgg aggcgagtgg ctgcttcccg cgcaggtagg cgaggatggc cggatcgtcc
      541 tgcgcccgcc ggctcatcgg cgtcgagacc agggccggcg cgatcgcgtt gacgcggatg
      601 ccgcgcggcg cgtagaaggc cgcggtggcg cggacgaacg cctcgatcgc gcccttcgac
      661 gcggcatagg cgtgggtcgc gaagtactcg gcggacgggt ggcgcgagag cccgctcgac
      721 atggccagga tcgtcccgcg ttggctatcg gcatccggtt cctgctcgag catcgcgcgg
      781 accgccgccc gggacaccag gaagaggctc gtcaggttcg acccgatgac cgtctcccag
      841 ccctcgagcg tggcctcgtg cagcggaccg tcgccgaagc gacgcccgga gatgccggcc
      901 accgcgtaga ccgcgtccag gcgtccgagg cgatcccgcg ccgcggcgac ggcggcctcg
      961 gcgccgtcct cctgggacag ctcggcggcg aaccagccgt gacgtccgcc cagctcgtcg
     1021 cccagtgctc gagcgtggtc ggcggtccgt gatgcaacga acacccgccc gccctccgcg
     1081 gcgatggctc gagccgcgga tgccgccatc cccgtcgagc cggtgatcag cacggcccgt
     1141 ccgtcgaagc ggctcaccgc ggcgcctcgc gcaccgcgtc gatcagggca cgtcggccgg
     1201 ccgcatcggc cctggtgagg gagctgccga tgcccacggc gaccgcgccc gcggcgaggt
     1261 aggcggccgc gttggtggcg tcgatgccgc cggtcgggat gagctcgacg tccggcagcg
     1321 gcccgcgcag ctcgcgcacc atggccggac cgaccgcgga ggcggggaag agcttcacga
     1381 acgcggcacc cgcctcccag gccgttcgaa tctcggtcgg cgtcatcgcg cccgggacga
     1441 acggaaggcc gctggcgcgc gccgcgtgca gcagctgagg gtcgaggacc ggtgccacgc
     1501 cgaagtcggc gcctgccgcg acggccgcct cgagctgcgt cgaggtgagg atcgtcccgg
     1561 cgccgacgat ggagggctcg ctctgacgcg aggccaggag cgcgcggacg gcgaccaggt
     1621 catcggcgcc atccggcgcg tcgagcgtga tctcgaacac gcggacgccg gcgtccgcaa
     1681 gctcctcgac cagcgccagc agcgtcgagc gcggctgcac ctgacgcagg accgcgatga
     1741 gccgatgccg ccgcaccgca tcggcgacgt cgccggaccg cagcgcctcg ctcacggcgc
     1801 gacctccgcg ggcgccaggg gctcgagcag tgcgccgtca tcggccaggc gcgagcgcag
     1861 cgcgccgcca tggacctcgc gcggcgaaca gcgggctcga ccagccagcg cggcggcggt
     1921 tccggccgcc tggcccatgg ccatgcaggt ggccatcgac cgtgcggagg cgtgggcgtc
     1981 gtggctggcc gagaagcatc ggcccgcgac cagcagcccg tcgagaccgg ccggcagcag
     2041 cgcgcggtag gggatgccgt acacctcgcc gccaggcacg tactgccact cggtgtcgcc
     2101 gccggcgtgg tggtcctcga tcggcgcgcc gcacaacgcg atctcgtcgt cgaagcgcgc
     2161 ggcgtacagc acgtcgtcgc gggtcagcac gtaatcgccg tggacgcgcc gcgactcgcg
     2221 gacgccgatg gcgggcgacg tggcgaccac caccgaggac tcgaagcccg gcacccggtc
     2281 gcgcaggaag cgcgcgtact cgcggacctg tcgccggccg tcgatctccg cgcgggtcag
     2341 ctgctcggga tcggtggcgt cg
//
LOCUS       DASTYP010000237         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_754552, whole genome shotgun sequence.
ACCESSION   DASTYP010000237 DASTYP010000000
VERSION     DASTYP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754552"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..731
                     /locus_tag="VFV59_10160"
     CDS             6..731
                     /locus_tag="VFV59_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEU5326233.1"
                     /translation="MCYTDAYARDVDATVVAVDPEAHAALLDRTVFYPGGGGQPADAG
                     ELVAAAGGSWVVTGAKKRGDDIWHTIEEEAELPAVDTQVTASIDWERRHRLMRTHSAL
                     HVLCGVVWRDHQASVTGGNMEPLSGRMDFEFETMRAELVAEIERRVNEEIAADREIRV
                     AILPREEAFAIPDLIRTKINLLPEGISEVRTIEIVGLDLQADGGTHVARTSEIGRVKV
                     TGYESKGRINKRIRIEVTDATDA"
     gene            724..1203
                     /locus_tag="VFV59_10165"
     CDS             724..1203
                     /locus_tag="VFV59_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU5326234.1"
                     /translation="MPDDPRTPAIVAVEAAGIPHRVVGTQPARNPEESAELQGIPLGA
                     LLRTIVVRRGADDYVFVLVPGGRRFDWPKLRAHLGVNRLSLPDADEARAVTGYERGAI
                     TPFGASRAWPVIADASALKPETVAIGGGARGVNLHLDPRVLVEHLHAEIADVTVAER"
     gene            1331..1768
                     /locus_tag="VFV59_10170"
     CDS             1331..1768
                     /locus_tag="VFV59_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEU5326235.1"
                     /translation="MRLTSEAFQDGGAIPARYTCDGEDVSPPLAWDGVPDGTQALAIL
                     VEDPDARGFVHWVLADIPGEALAMAEGEGDAVGTPGRNDFRRSGWGGPCPPSGEHRYV
                     FTLYALSQPVGLPEGTDAGAVRSRMEGHVMATATLTGVYRRNR"
     gene            1829..>2147
                     /locus_tag="VFV59_10175"
     CDS             1829..>2147
                     /locus_tag="VFV59_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEU5326236.1"
                     /translation="MPARDELPGTLRRSPRKAQDTWSETHDSAVESYGEGERAHRTAY
                     ASLKHSFEKIGDHWEPKGERGPSDPRAAKGGAAARRGEGETFGGVDVLGNTKADLYER
                     ARKL"
BASE COUNT          338 a          737 c          803 g          269 t
ORIGIN      
        1 agaacatctg ctacaccgat gcttacgccc gcgatgtcga cgcgaccgtc gtcgcggtcg
       61 accccgaggc gcacgcggcc ctgctggacc ggaccgtctt ctacccgggt ggcggcggcc
      121 agcccgcgga tgccggcgag ctcgtggccg cggccggcgg ctcgtgggtg gtcaccggcg
      181 cgaagaagcg gggcgacgac atctggcaca ccatcgagga ggaggcggag ctgccggcgg
      241 tcgacacgca ggtcacggcg agcatcgact gggagcgtcg gcaccggctg atgcgcacgc
      301 actccgccct ccacgtgctg tgcggcgtcg tgtggcgcga ccaccaggcg agcgtcaccg
      361 gcggcaacat ggagccactg tccggccgca tggacttcga gttcgagacg atgcgggccg
      421 agctggtggc cgagatcgag cgacgcgtga acgaggagat cgccgcggat cgcgagatcc
      481 gggtcgccat cctgccgcgc gaggaggcct tcgcgattcc ggacctgatc cgcaccaaga
      541 tcaacctgct gcccgagggc atcagcgagg tgcgaacgat cgagatcgtc gggctcgacc
      601 tccaggccga tggcgggacg cacgtggcac ggacctcgga gatcggccgc gtgaaggtca
      661 ccggctacga gtcgaagggc cggatcaaca agcggatccg catcgaggtg accgacgcga
      721 ccgatgcctg acgatccgcg cacgccggcg atcgtggcgg tggaggcggc cggcatcccc
      781 caccgcgtcg tcgggacgca gccggcgcgc aacccggagg agagcgcgga gctgcagggc
      841 atcccgctcg gggcgctgct gcgaaccatc gtcgtgcggc gcggtgccga cgactacgtc
      901 ttcgtcctgg tccccggcgg acgccgcttc gactggccga agctgcgcgc gcacctcggc
      961 gtcaaccgcc tctccctgcc cgatgcggac gaggctcgcg ccgtcacggg ctacgagcgc
     1021 ggcgccatca cgcccttcgg cgccagccgg gcctggccgg tgatcgcgga cgcctcggcg
     1081 ctgaagccgg agaccgtggc gatcggtggc ggcgcgcgcg gggtcaacct ccacctcgac
     1141 cctcgcgtgc tggtcgaaca ccttcacgcc gagatcgccg acgtgacggt cgcggagcgg
     1201 tgatgcgatg cgaggccggc ctggactgat ccagacggct ctcgccatcg gcctctccct
     1261 cgcggcatgc tcggctggcc cgagcgatgc agccacgacc tcgggcgatg ctcaggaggg
     1321 accgacgacc atgcgactca ccagcgaggc cttccaggac ggcggggcca tcccggcgcg
     1381 gtacacctgc gacggcgagg acgtctcccc gcccttggcc tgggatggcg tacccgacgg
     1441 gacgcaggcc ctcgccatcc tggtcgagga cccggacgcg cgcggcttcg tgcactgggt
     1501 cctggcggac attcccggcg aggcgcttgc catggccgaa ggcgagggcg acgcagtcgg
     1561 gacgccgggg cgcaacgact tccgccgcag cggctggggc gggccgtgcc cgccgtccgg
     1621 ggagcatcgg tatgtcttca cgctgtacgc cttgtcccag ccagtcggcc tcccggaagg
     1681 gaccgatgcg ggcgcggtgc gctcgcggat ggaggggcac gtcatggcca ccgccacgct
     1741 gaccggggtg taccggcgga atcggtgagg cgaaccggca gcgaacctgc atcggttcga
     1801 agcggaacga accgatggag gtaccgccat gccggcacgt gatgagctcc cgggcacgct
     1861 gagacgctcg cccaggaagg cgcaggacac ctggagcgag acccacgaca gcgccgtcga
     1921 gagctacggc gagggcgagc gggcgcatcg gacggcgtac gcgagcctga agcactcctt
     1981 cgagaagatc ggcgaccact gggagccgaa gggcgagcgc gggccgtccg accctcgggc
     2041 cgccaagggc ggagccgcgg ctcgtcgtgg tgagggcgag acgttcggcg gtgtggacgt
     2101 cctcggcaac acgaaggcgg acctgtacga gcgggcgcgg aagctgg
//
LOCUS       DASTYP010000238         4801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_899505, whole genome shotgun sequence.
ACCESSION   DASTYP010000238 DASTYP010000000
VERSION     DASTYP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899505"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFV59_10180"
     CDS             complement(<1..976)
                     /locus_tag="VFV59_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEU5326237.1"
                     /translation="MSHTRVDGGDGREAIALEERSPLQLTAATATDIWNDSCAVDELE
                     YAIAHGAVGATANPTIVYDTWKKDPVRWGSRVRELAREHPRATEADLAWLIVEEMSAE
                     GATLLLPAYEHHAGRQGRLSMQTNPQFWRSAEPMLAQGVHFTSVAPNIIVKFPATSVG
                     IGVMEEATYRGVSVNCTVSFSVAQAVAAAEAVERGLERREREGLPTGDMGPVITIMVG
                     RIEDWLRVQAERDRISVHPSALPWSGVAVFKRAYQLFRERGYRARLLAAAIRHHLHWS
                     EFIGGDVVITLPSAWQKRFNASGVEVLERMDEPVDPAYVDELVRRFDDF"
     gene            complement(973..2775)
                     /locus_tag="VFV59_10185"
     CDS             complement(973..2775)
                     /locus_tag="VFV59_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEU5326238.1"
                     /translation="MPGPRRKAMAVDAQEQVLDQFLGSDEFPIEWRSEREKQLFWVFD
                     DLHCPHPLSPMFYEIGGWWLTCDHMFRRFGTPFACDWVTKNVNGYLYTAAVPADADWA
                     VDAMEYGYRYGARVPVGGDYAETIGSYLDAALPVYGEHFVDWWRGRLVPEMERNFAFL
                     EGELARQDDMSVMELAVLLEDAIDVHDRHWKIHWMLNFAQLSATLKLRAVMEKTHGRV
                     DEALLGRLQNSASDRNWDSIKALWEMKEEAKADPELAKAFSHDTAGEIVAALDGNERG
                     RRFVAERVKPYQQEFGWHAVWSHEFVFPTVYEKMEPVIELVRGYLETDYDYPSTMGAL
                     KADIEAASREILEGLEGEALDEMRAANETNLQMAPLTPDHHFYIDQGANAHVRLVLIA
                     IGRKLVERGTLDEPDDVIFLRYNELRVVMADASRIDARALVKQRREERERAYTFRPPA
                     WIGTATQSQLDFPYLTNWGFPEKFHRKPGEASDRVTGIGGSPGVVEGVARVVLREDQF
                     DEVRAGDILVCHMTNPAWVVLFTKIAGLVTNAGGLTAHPAVLSREFGIPAVVGTQDGT
                     QRINTGDRLRVDGGKGVVEIVEAAAQPKNDLFTA"
     gene            2868..>4801
                     /locus_tag="VFV59_10190"
     CDS             2868..>4801
                     /locus_tag="VFV59_10190"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEU5326239.1"
                     /translation="MHASSSRYVFAFDDPPAADADARLRLLGGKGAGLVTMSTDLGLP
                     VPPGFTITTEACREYLAGRWPESLDAEVRSAMLRLGDQVGRRFGDPDDPLLVSVRSGA
                     PVSMPGMMDTILNLGLTADGVRGLAAATGDERFAADCLLRLRDGYRAVIGADEPPDDP
                     WEQLREAIEAVFRSWNSERARAYRRREGIDESLGTAVTVQAMVFGNRGTDSGTGVLFT
                     RDPSTGVAEPYGDVMFDAQGEDVVAGAHEPQPIAALDERLPAVAEELRRHAARLERHV
                     ADLCDIEFTIEQGRLWMLQVRAGKRSPRAALRMAIEMASDPEFPLTREDAVRRVLRHL
                     ADPPRVFVGAGDRPTPLTTGLPASPGVASGEIAVSSSEAEAAAADGRPVILVRAETSP
                     EDVPGMARSVGVLTTRGGLASHAAVVARGWGIPAVVGASEVRIGDGVVRVGDVELGAG
                     ARLTIDGSTGEVHAGDLEGSWEVAPEAATLLAWAQELGIEVPASPVEDGAPVSPAKQV
                     ELGPTDLTADEVLRVAMIKGGGSVDQLAEALLVESDAGRPLVDELIRNGAAEDAGAAI
                     RLTSSGKLAALDAFARDRAQFGERRADEALAGFHDFDARMKEIVTGWQVREIDGEQAL
                     NDHTDPAYDAEILDQLARLH"
BASE COUNT          679 a         1727 c         1668 g          727 t
ORIGIN      
        1 ggaaatcgtc gaagcgtcgc accagctcat ccacgtacgc ggggtcgacc ggctcgtcca
       61 tgcgctccag cacctcgaca ccggacgcgt tgaagcgctt ctgccaggcc gatggcagcg
      121 tgatgacgac gtcgccgccg atgaactcgg accagtgaag gtggtgccgg atggcggcgg
      181 ccagcagccg ggcgcgatag ccgcgctcac ggaacagctg ataggcgcgc ttgaagacgg
      241 cgacgccgga ccatggcagc gccgatgggt gcacgctgat gcggtcgcgc tcggcctgca
      301 cgcgcagcca gtcctcgatc cggcccacca tgatggtgat caccggtccc atgtctccgg
      361 tgggcagccc ctcgcgctcg cgccgctcca ggccgcgctc gaccgcctcg gccgcggcca
      421 ccgcctgcgc caccgagaac gagacggtgc agttgaccga gacgccccgg tacgtcgcct
      481 cctccatcac gccgatgccg acggacgtgg ccgggaactt cacgatgatg ttcggcgcga
      541 ccgaggtgaa gtggacgccc tgcgccagca tcggctccgc gctgcgccag aactgagggt
      601 tggtctgcat cgacagccgc ccctggcgac cagcatggtg ctcgtaggcc ggcaacagca
      661 gcgtcgcgcc ctcggcggac atctcctcca cgatcagcca ggcgaggtcc gcctcggtgg
      721 cgcgcgggtg ctcgcgcgcc agctcgcgca cgcgtgagcc ccatcggacc gggtccttct
      781 tccaggtgtc gtatacgatc gtggggttcg cggtcgcgcc gaccgccccg tgggcgatcg
      841 cgtactcgag ctcgtccacg gcgcacgagt cgttccagat gtcggtggcc gtggccgcgg
      901 tgagctgcag cgggctgcgc tcctcgagtg cgatcgcctc ccgcccgtcc ccgccgtcga
      961 ccctcgtgtg gctcatgccg tgaagaggtc gttcttcggc tgcgcggctg cctccacgat
     1021 ctcgacgacg cccttgcccc cgtcgacgcg gaggcggtcg ccggtgttga tccgctgcgt
     1081 cccgtcctgc gtgccgacga cggccgggat cccgaactcg cggctcagga ccgccgggtg
     1141 ggcggtgagc ccgcccgcgt tggtgacgag gcccgcgatc ttcgtgaaga gcaccaccca
     1201 cgcggggttg gtcatgtggc agaccaggat gtcgcccgcc cgcacctcgt cgaattggtc
     1261 ctcgcgcagg acgacccgcg cgacgccctc caccaccccc ggggatccgc cgatgccggt
     1321 cacccggtcg gaagcctcgc ccggcttgcg gtggaacttc tccgggaagc cccagttcgt
     1381 caggtacggg aagtccagct ggctctgcgt cgccgtaccg atccaggccg gcggtcggaa
     1441 ggtgtacgcg cgctcccgct cctcgcgccg ctgcttcacc agggctcgag cgtcgatccg
     1501 ggacgcatcg gccatgacga cgcgcagctc gttgtagcgc aggaagatca cgtcgtcggg
     1561 ctcgtccagc gtgccgcgct ccaccagctt ccggccgatg gcgatcagca ccagccgcac
     1621 gtgcgcgttg gccccctggt cgatgtagaa gtggtggtcg ggcgtgagcg gagccatctg
     1681 caggttcgtc tcgttcgcgg cgcgcatctc gtcgagcgcc tcgccctcga ggccctccag
     1741 gatctcgcgc gaagcggcct cgatgtcggc cttgagcgct cccatggtcg acggatagtc
     1801 gtaatcggtc tccaggtagc cgcggaccag ctcgatcacc ggctccatct tctcgtacac
     1861 cgtcgggaag acgaactcgt ggctccagac cgcgtgccag ccgaactcct gctggtaggg
     1921 cttcacccgt tcggcgacga accgtcgccc gcgctcgttc ccgtcgagcg ccgccacgat
     1981 ctcgccggcg gtgtcatggc tgaaggcctt cgccagctcc ggatcggcct tcgcctcctc
     2041 cttcatctcc cacagcgcct tgatcgaatc ccagttgcgg tcgctggccg agttctgcag
     2101 ccgtccgagc agcgcctcgt ccacacgacc gtgggtcttc tccatcacgg cgcgcagctt
     2161 cagcgtggcc gacagctggg cgaagttgag catccagtgg atcttccagt gccggtcgtg
     2221 gacgtcgatc gcgtcctcca gcaggacggc gagctccatg acgctcatgt cgtcctgccg
     2281 cgccagctca ccctccagga aggcgaagtt gcgctccatc tccggcacca gccggccgcg
     2341 ccaccagtca acgaaatgct cgccgtacac ggggagcgcg gcgtccagat agctgccgat
     2401 ggtctcggcg tagtcgccgc ccaccggcac gcgggcgccg tatcggtagc cgtactccat
     2461 ggcgtcgacc gcccaatccg cgtcggccgg gacggcggcg gtgtagaggt agccgttgac
     2521 gttcttggtg acccagtcgc acgcgaacgg cgtcccgaag cgacggaaca tgtggtcgca
     2581 cgtcaaccac cagccgccga tctcgtagaa catcgggctc agcggatggg ggcagtggag
     2641 gtcgtcgaag acccagaaca gctgcttctc gcgctccgag cgccactcga tcgggaactc
     2701 gtcgctgcca aggaactggt ccagcacctg ctcctgcgca tccaccgcca tcgccttcct
     2761 ccgtgggccg ggcatcctat cgatgatcga tcataatgac cgatagctcc catcgccgcc
     2821 agtatccgac gccggcggcg cgcacgcgcc aggagtctcc cagaccgatg cacgccagct
     2881 cttcgcgata cgtcttcgcc ttcgacgatc ccccggcagc cgacgccgat gcgcggctcc
     2941 ggttgctcgg cgggaagggc gccggcctcg tcaccatgtc gaccgacctc ggcctgccgg
     3001 tcccgcctgg attcaccatc accacggagg cgtgccgcga gtacctggcc ggacgctggc
     3061 cagagagcct cgatgcggag gtacgctccg ccatgctgcg cctgggcgac caggtcgggc
     3121 gccggttcgg cgacccggac gatccgctgc tggtcagcgt gcgctccgga gcgccggtgt
     3181 cgatgcccgg catgatggac acgatcctga acctcggcct caccgcggac ggcgtgcgcg
     3241 ggctcgcggc cgccacgggc gacgagcggt tcgcggccga ctgcctgctg cggctgcgcg
     3301 acgggtaccg cgccgtcatc ggcgcggacg agcctcccga cgacccgtgg gagcagctgc
     3361 gggaagcgat cgaagcggtg ttccgctcct ggaacagcga gcgggcccgt gcctaccgcc
     3421 gtcgcgaggg gatcgacgag tcgctcggga ccgcggtgac ggtccaggcc atggtcttcg
     3481 gcaaccgcgg cacggactcg gggacgggcg tcctcttcac ccgcgacccg tcgaccggcg
     3541 tcgcggaacc gtacggcgac gtcatgttcg acgcccaggg cgaggacgtg gtggccggcg
     3601 cccacgagcc gcagcccatc gccgcgttgg acgagcggct gccggccgtc gccgaggagc
     3661 tgcgccggca cgcggcgcgg ctggaacgtc acgtcgccga cctgtgcgac atcgagttca
     3721 ccatcgagca gggccggctc tggatgctcc aggtgcgcgc cggaaagcgc agcccgcgtg
     3781 ccgcgctgcg catggcgatc gagatggcct cggatcccga gttcccgctc acgcgcgagg
     3841 acgccgtccg ccgcgtgctg cgccacctgg ccgatccgcc gagggtcttc gtcggcgccg
     3901 gcgaccggcc gacgccgctg acgaccggcc ttcctgcgtc gcccggcgtc gcgagtggcg
     3961 agatcgccgt ctcgtcgtcc gaggccgagg cggccgccgc cgacggccgg ccggtcatcc
     4021 tggtccgtgc cgagacctcg cccgaggacg tgccgggcat ggcgcgctcg gtcggcgtcc
     4081 tcacgacgcg cgggggattg gccagccacg cggccgtcgt ggcgcgcggc tggggcatcc
     4141 cggccgtggt cggggcgtcc gaggtgcgca tcggtgacgg cgtggttcgc gtcggcgacg
     4201 ttgagctggg cgctggcgct cggctcacca tcgacggcag cacgggcgag gtccacgccg
     4261 gcgacctgga ggggagctgg gaggtcgcgc ccgaggcggc gacgctgctg gcctgggcgc
     4321 aggagctcgg catcgaggtc ccggcgtcgc cagtcgagga tggggcgcca gtgtcacccg
     4381 ccaagcaagt cgaactcgga ccgacggacc tgaccgcgga cgaggtcctt cgggtggcca
     4441 tgatcaaggg cggcgggtcc gtggatcagc tcgccgaggc cctgctcgtc gaatcggatg
     4501 cgggccgtcc gctcgtggac gagctgattc gcaacggtgc ggcagaggat gccggggcgg
     4561 cgatccgcct cacctcgtcg ggaaagctcg cggccctcga cgccttcgcc cgtgaccgcg
     4621 cgcagttcgg cgagcggcgc gccgacgagg cgctggcagg gttccacgac ttcgacgcgc
     4681 ggatgaagga gatcgtgacg gggtggcagg tgcgggagat cgacggggag caggcgctca
     4741 acgaccacac cgatccggcg tacgacgccg agatcctcga ccagctcgcg cggctgcacg
     4801 a
//
LOCUS       DASTYP010000239         7163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1665686, whole genome shotgun sequence.
ACCESSION   DASTYP010000239 DASTYP010000000
VERSION     DASTYP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665686"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFV59_10195"
     CDS             complement(<1..855)
                     /locus_tag="VFV59_10195"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5326240.1"
                     /translation="MPRRPKTPDVTEPLFEAGTAPDAPLAARMRPRTIEEFVGQAHLV
                     GPEGALTRVVRPGHLPSFVLWGPPGSGKTTLARLLAERSGGSWRPLSAVTSGVADIRS
                     LVAEAKALRSAGGRTVAFIDELHRFNKSQQDALLPHVEDGTIALIGATTENPYYEINS
                     PLLSRMRVYRLEPLDADALGDIVDRALTDADRGLGGRVTLTDEGRATLLDLAGGDARQ
                     ALNVLESAAVTAGEGGRLGPDEIAEAAQHRLLAYDRVGDQHYEAASAFIKSMRGNDPD
                     AALYWMASM"
     gene            904..2172
                     /locus_tag="VFV59_10200"
     CDS             904..2172
                     /locus_tag="VFV59_10200"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5326241.1"
                     /translation="MTFAAGWDQRDRRTLTFVDADLERAFQAAAADPARRRLVLNAVA
                     NIPVWIIGSVAAGLVIQGIYDRPIWLMALGQCLGLAAGGVWALRASRSRRAVDLVYLA
                     LDLSGGLVLTVMAVVSGLFESYGAFLLLFTAVNALAVQRVSFIPSVIAIMGQLAMFTV
                     AALAIGMAALPFQFVLLASALAVLVAGNYMVEDSERREFAQARLIAALHDQVDDLLHR
                     YLEPGVVDSIIAEPSRGELGGEQMEVSVMFADLTGFTDYSDRVEPAAAMALLNESFAA
                     AVPVVRAAGGTIVQFAGDAMMVIWNAPRAQPDHACLAARAAIGLQRATAHFAEDPAHP
                     RFRVGLNTGPALVGNIGTAELKDFTAIGDTTNLAARLQSIAAPGSIVIGPYTRELLGD
                     RATVHDLGLHAVKGKATSVRLYELVDMTEA"
     gene            2202..3359
                     /locus_tag="VFV59_10205"
     CDS             2202..3359
                     /locus_tag="VFV59_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU5326242.1"
                     /translation="MEQNLRTPGPTPLPPSVREAQAAQMINHRGPEFAEMLHEISSGI
                     RDLIGASGDVLLLTGSGSGAMEAAIVNTLSPGDRVLAVSIGSFGERFAHIASAFGAAV
                     DVLEFEWGTAADPDVLRDRLASSEPYRAVLITHNETSTGVANPLEALARVVREAPGDP
                     LLLVDGISGLGAMPFEMDAWGVDLVVSASQKAWMASPGIAIAAVGERARAAGASARMP
                     RVYWDFEEARRWAAKGQTPWTPAISVLYGLRVGVSSLVAEGRERTWDRHRAVAEGVAA
                     GLDRLGLSLVAPTGHRSPTVTAAWVPEGVEWAAVNAALLARGLVLAGGQGKWTGRILR
                     FGHMGEISIHEMADGLELLGEELTALGHQSDGAAAADAARSAFEAAVLATR"
     gene            3356..4030
                     /locus_tag="VFV59_10210"
     CDS             3356..4030
                     /locus_tag="VFV59_10210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5326243.1"
                     /translation="MTAARLVIVRHGVTAWNREGRFQGHLDPPLSTAGRREAQLVARR
                     IAEDDDLRPTRIVSSSLARAAETAESIASVCAVSVERDDRLMEIGQGRWEGRTHDEIA
                     AEDPARYQRWRTATGLKRPPGGETAASATGRLASFVANLAEGGPGDETVCVVSHGGSI
                     RILAALLFDIGLERAWALDVDNASLSVASRGTRGWRVERWNDTHHLLGLEATHVDEAD
                     GRPLAL"
     gene            complement(4052..6355)
                     /locus_tag="VFV59_10215"
     CDS             complement(4052..6355)
                     /locus_tag="VFV59_10215"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEU5326244.1"
                     /translation="MPDLRERILYPAAGWLSLGLLAVMVLALGWSVQGAGWLDQLGFL
                     VPVGLWALAIGAVLGVLGWTIVLALPLAAVSGAAIILWTIGSEYYPVLGQGDRLLALR
                     TDALDWLAILLDTGYPTQMAPYAIGLGVLMFATAFMAGYAVYRYHRVLDAILLLGTAL
                     IVNMSATYTDLFGHLLLFVIAALLLWLRAALVTRREGWQRRRVNENLEVPAAIMRSGI
                     VFAAASVALAWVLTSVAVAAPLTDAWRSFDGVWTGVKEQFDGVFGSLTNPQSRITGTS
                     FGPGFAVRGEWFSNNEEALVLAASRPLYLRTATYDVYTGRGWERSDGPKRQVAAGEQL
                     FDVATSERPTVRESVEVETITIQMRQALGRNLFTAGSPLKVYAPVVIHESGGQPVLGA
                     IDAANAFGAGEAYQVSVAISEATEAELAVAGTDYPASVRALYLSTPGITDRVRQLAED
                     LTADAANPYEKAKALARYLRSDDSFHYETRGAIPKSGQDLVDFFLFDEAANRTGYCEY
                     YASAMVVMARAVGLPARVAVGFAPGEETEDGTYLVRESNAHAWAEIYFPGYGWQIFEA
                     TKTINPRFTRPTGDPTTVGPPVRGRELDPLAEWELGQGDDAVTTLPSFDPIAGGFDAT
                     QGQPKPEAEQAREGNAALILALVALGGLAVWWRVRTLERRWRLLPAGDRAWRQLTAAA
                     QRAGVGPRPSETIYEYAGWLEEQLPSQREPIRTVADGKVLQAYSGRRMTRTAGDRLDS
                     AMARLRLPLMGLAVRRGVRRLLRRDAG"
     gene            complement(6348..>7163)
                     /locus_tag="VFV59_10220"
     CDS             complement(6348..>7163)
                     /locus_tag="VFV59_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5326245.1"
                     /translation="FGLFSAEMAVGQPTGVVVFPEVVPLPHWRLPPSPIDGTTRTKRR
                     YEAATPLVAGVRPYTHGDAINRIHWLSSARHGELHVKEFDLEQAADLWILLDLDRAVH
                     AGVGTDASVETAVTIAASIAMRTLSDNRSVAMTASARRLQLHQPDRGPRVEQKLLHLL
                     ANVQADGSTPLAEVLNTTLTQLRRGMTLCIVTGSTDRTWVRALAALRRRGVGALVILL
                     DRASYIGVDPDDRARSELMAVRHALAEYDVEHRLVRRGDDIAEAIGGRVRGRA"
BASE COUNT          996 a         2597 c         2553 g         1017 t
ORIGIN      
        1 catcgacgcc atccagtaca gcgccgcgtc aggatcgttg ccccgcatgc tcttgatgaa
       61 ggccgatgcg gcctcgtagt gctgatcccc cacccggtcg taggccagca gccgatgctg
      121 cgctgcttcg gcgatctcgt ccggccccag ccggccgccc tccccggccg tgacggcggc
      181 ggactccagg acgttcagcg cctggcgggc atcgccgccc gccaggtcca ggagcgtcgc
      241 ccgaccctca tcggtcagcg tgacgcgccc gcctaggccg cgatcggcat cggtcagcgc
      301 ccggtcgacg atgtcgccga gcgcatcggc gtcgagcggc tccaggcgat agacgcgcat
      361 ccgggacagc agcggcgaat tgatctcgta gtacgggttt tccgtcgtgg cgccgatgag
      421 cgcgatggtg ccgtcctcga cgtgcgggag cagggcgtcc tgctggctct tgttgaatcg
      481 atgcagctcg tcgataaagg cgaccgtccg cccgccggcc gatcggagcg ccttcgcctc
      541 ggccaccagg gagcggatgt cggcgacgcc gctcgtcacc gcggacaatg gacgccacga
      601 tccgcccgag cgctcggcca gcagccgcgc caacgtcgtc ttgcccgagc ccggcggtcc
      661 ccacagcacg aacgagggca ggtgtcccgg tcgcaccacc cgcgtcagcg ctccctcggg
      721 cccgacgagg tgcgcctgcc cgacgaactc ctcgatggtc cgcggacgca tccgcgcggc
      781 cagcggggca tccggggcgg tgcccgcctc gaacagcggc tccgtcacgt ccggggtctt
      841 ggggcgtcgc ggcatgctgg aattatggtc cgaggccccg cggcgacgcc gtagagtccg
      901 cccgtgacgt ttgccgcagg ttgggatcag cgcgaccgcc gcacgctgac cttcgtcgat
      961 gcggacctcg agcgcgcctt ccaggcggcg gcggcggacc ccgcgcggcg ccgcctggtc
     1021 ctgaatgccg tcgccaacat cccggtctgg atcatcggat cggtcgccgc gggcctggtc
     1081 atccagggca tctacgatcg tccgatctgg ctgatggccc tcgggcagtg cctgggcctc
     1141 gcggcgggtg gagtctgggc ccttcgggca tctcgttcgc gacgggcggt ggacctggtg
     1201 tacctggcgc tggacctgtc gggcggactc gtgctgaccg tgatggccgt cgtgtccggc
     1261 ctgttcgagt cctacggcgc gttcctgctc ctcttcacgg ccgtcaacgc gctggccgtc
     1321 cagcgcgtgt cgttcatccc gtcagtcatc gccatcatgg gccagctcgc catgttcacc
     1381 gtggcggctc tggccatcgg catggcggcg ttgccctttc agttcgtgct gctggcctct
     1441 gccctggcgg tgctggtcgc cggcaactac atggtcgagg acagcgagcg gcgagagttc
     1501 gcgcaggccc gcctgatcgc ggcgctgcat gaccaggtgg acgacctcct gcaccgctac
     1561 ctggagccgg gcgtggtcga ctcgatcatc gccgagccgt cgcgcggtga gctgggcggc
     1621 gagcagatgg aggtgagcgt catgttcgcc gatctgacgg gcttcaccga ctacagcgat
     1681 cgggtcgagc ctgcggcggc gatggccctg ctcaacgagt cgttcgccgc cgcggtcccg
     1741 gtggttcgcg ccgcgggtgg cacgatcgtg cagttcgccg gcgacgccat gatggtgatc
     1801 tggaacgccc cgcgcgcgca gcccgatcac gcctgcctgg cggcgcgcgc cgccatcggc
     1861 ctgcaacgag caaccgccca cttcgccgag gatcccgccc atccccgatt ccgggtcggg
     1921 ctcaacaccg ggccggcgct ggtcggcaat atcggcacgg ccgagctcaa ggacttcacc
     1981 gccatcggtg ataccacgaa cctggccgcc cggctccaat cgattgccgc gccgggcagc
     2041 atcgtcatcg gcccgtacac ccgcgagctc cttggcgacc gggcgaccgt ccacgacctc
     2101 ggcctgcacg cggtgaaggg caaggcgacc tcggttcggc tgtacgagct ggtggacatg
     2161 accgaggcct gatccgttcc gcgccgacta tgatcgcgcg catggaacag aacctccgca
     2221 ccccgggtcc gacgccactg ccgccgtccg tgcgggaggc gcaggcggcg cagatgatca
     2281 accaccgcgg gccggagttc gcggagatgc tccacgagat ctcgtccggc atccgcgacc
     2341 tgatcggcgc gagtggcgac gtgctgctgc tcacgggctc cggctcgggc gccatggagg
     2401 cggccatcgt caacaccctc tcccccgggg atcgtgttct ggcggtgagc atcggctcct
     2461 tcggggagcg gttcgcccac atcgcgtcgg cgttcggcgc ggccgtcgac gtcctcgagt
     2521 tcgagtgggg caccgccgcg gatcccgacg tgctccgcga ccgcctcgcc agctcggaac
     2581 cctaccgggc ggtgctcatc acccacaacg agacctcgac cggagtcgcc aacccgctcg
     2641 aggcactcgc gcgcgtcgtg cgcgaggcgc ccggggatcc gctgctcctc gtcgacggca
     2701 tcagcggcct cggggccatg ccgttcgaga tggacgcgtg gggcgtcgac ctggtggtga
     2761 gtgccagcca gaaggcctgg atggcgtcac cgggcatcgc gattgcggcg gttggcgagc
     2821 gcgcgcgcgc cgcgggcgca tcggcccgca tgccgcgcgt gtactgggac ttcgaggagg
     2881 cgcgacgctg ggcggccaag ggacagacgc cgtggactcc ggccatcagc gtgctctacg
     2941 gcctgcgcgt cggggtcagc agcctcgtcg ccgagggacg ggaacgaacg tgggatcggc
     3001 atcgcgccgt ggccgagggc gtggccgccg gcctcgaccg gctggggctg tcgctcgtcg
     3061 ccccgaccgg ccaccgctcg ccgaccgtca ccgcggcatg ggtccccgag ggggtcgagt
     3121 gggcggcagt caacgccgcg ctgctggcgc gagggctggt gctggccggc ggacagggca
     3181 agtggaccgg acgcatcctg cgcttcggcc acatgggcga gatcagcatc catgagatgg
     3241 ctgatgggct cgagctgctc ggcgaggagc tgaccgcgct cggtcaccag tccgacggcg
     3301 cggccgcggc cgatgccgcc cgctccgcct tcgaggcggc ggtcctcgcc acccggtgac
     3361 ggcggcgcgg ctggtcatcg tccgccacgg cgtcacggcc tggaaccgcg agggtcgctt
     3421 ccagggccac ctcgacccgc cgctcagcac cgccggccgc cgggaggccc agctggtcgc
     3481 ccgacgcatc gccgaggacg acgacctgcg gccgacgcgg atcgtctcgt cgtcactggc
     3541 gcgagccgcc gagaccgcgg aatccatcgc gagcgtatgc gcggtctcgg tcgagcgcga
     3601 cgaccgcctc atggagatcg ggcagggccg ctgggagggt cggacgcacg acgagatcgc
     3661 agccgaggac ccggcccgct accaacgctg gcgcacggcc acggggctca agcgtccgcc
     3721 gggcggtgag accgccgcat cggccaccgg gcgcctggcg tcgttcgtcg cgaacctcgc
     3781 cgaaggtggg ccgggcgatg agacggtgtg cgtcgtgagc catggcggct cgatccggat
     3841 cctggccgcg ctcctgttcg acatcggcct cgagcgggcg tgggcgctcg acgtcgacaa
     3901 cgcctcactc agcgttgcct cgcgcggcac gcggggctgg cgcgtggaac gctggaacga
     3961 cacgcaccac ctgctcggtc tcgaggcgac ccatgtcgac gaggccgatg ggcgtcccct
     4021 cgccctctga cgtcagccga gccgcgggtc cctagcccgc gtcgcgccga agcagtcggc
     4081 gcacgccccg ccgcacggcg aggcccatca gcggcagccg caggcgcgcc atcgcggagt
     4141 cgagccgatc gccggcggtt cgcgtcatgc ggcgcccgga gtacgcctgg agcaccttgc
     4201 cgtccgcgac cgtccggatc ggctcccgct ggctgggcag ctgctcctcg agccagccgg
     4261 cgtactcgta gatcgtctcg gacggtcgtg gaccgacccc cgcgcgttgc gcggccgccg
     4321 tcagctgacg ccacgcccga tcaccggccg gaaggagccg ccagcgccgc tcgagggtcc
     4381 gcacccgcca ccacacggcc agcccgccga gcgcgaccag cgcgaggatg agcgccgcgt
     4441 tcccctcgcg ggcctgctcg gcttcgggct tcggctgccc ctgcgtcgcg tcgaagccac
     4501 cggcgatcgg gtcgaacgac ggcagcgtcg tgacggcatc gtcgccctgg cccagctccc
     4561 actccgccag cgggtcgagc tcgcggccgc gaaccggcgg accgacggtc gtcggatcgc
     4621 ccgtcgggcg cgtgaagcgc gggttgatcg tcttggtcgc ctcgaagatc tgccagccgt
     4681 agcccgggaa gtagatctcc gcccaggcgt gggcgttgga ctcgcgcacc aggtaggtgc
     4741 cgtcctcggt ctcctcgccc ggcgcgaacc cgacggccac gcgggcgggc aggcccacgg
     4801 cgcgggccat cacgaccatg gcgctggcgt agtactcgca gtaaccggtg cggttcgcgg
     4861 cctcatcgaa gaggaagaag tcgaccaggt cctgcccgct cttggggatg gcgccccggg
     4921 tctcgtagtg gaacgagtca tcggatctca ggtagcgcgc cagcgccttg gccttctcgt
     4981 acgggttggc cgcgtcggcc gtgaggtcct cggccagctg ccgcacgcgg tcggtgatgc
     5041 ccggggtcga caggtacagc gcccgcaccg atgccgggta gtcggtgccc gccaccgcca
     5101 gctcggcctc ggtcgcctcg gagatggcga cgctcacctg gtacgcctcg ccggccccga
     5161 acgcgttggc ggcgtcgatg gcgcccagga ccggctggcc acccgactcg tggatcacga
     5221 ccggcgcata gaccttcagc ggtgagccgg ccgtgaacag gttgcgcccg agggcctggc
     5281 gcatctggat ggtgatggtc tcgacctcga ccgactcgcg cacggtgggc cgctcggagg
     5341 tggcgacgtc gaagagctgc tccccggccg cgacctggcg cttcggacca tccgatcgct
     5401 cccagccgcg accggtgtag acgtcgtacg tcgccgtccg gaggtagagc ggccgcgacg
     5461 cggcgagcac cagcgcctcc tcgttgttgc tgaaccactc gccgcggacc gcgaagcccg
     5521 gaccgaagct ggtgcccgtg atccgcgact gcgggttggt gagactgccg aagacgccgt
     5581 cgaactgctc cttcacgccg gtccacacac cgtcgaagct ccgccacgca tcggtcagcg
     5641 gagccgcgac agcgacgctc gtcagcaccc acgccagcgc gacggacgcg gcggcgaaga
     5701 cgatccccga ccgcatgatg gccgccggca cctccaggtt ctcgtttacc cgccgccgct
     5761 gccagccctc gcggcgcgtc accagggcgg cccgcaacca cagcagcagg gccgcgatga
     5821 cgaacagcag caggtggccg aagagatccg tgtaggtcgc cgacatgttg acgatcagcg
     5881 ccgtgccgag cagcaggatc gcgtcgagca cgcggtggta gcggtagacc gcgtagcccg
     5941 ccatgaaggc ggtcgcgaac atcagcacgc cgaggccgat cgcgtacggc gccatctgcg
     6001 tggggtatcc cgtgtcgaga aggatggcca gccagtcgag cgcatcggtg cgcagggcca
     6061 gcaggcggtc gccctggccg aggaccgggt agtactcgct gccgatggtc cacagaatga
     6121 tcgcggcgcc gctcacggcg gccagcggca gcgcgaggac gatcgtccag cccagcacgc
     6181 cgagcacggc gccgatggcc agcgcccaca agcccaccgg gaccaggaat cccagctgat
     6241 cgagccatcc ggctccctgg acactccagc ccagggcgag gaccatcacg gcgagcaggc
     6301 ccaggctgag ccagccggcc gccgggtaca ggatgcgctc gcgcagatca ggcacggccc
     6361 cggaccctcc cgccgatcgc ctccgcgatg tcgtcaccgc gacggaccag ccggtgctcg
     6421 acgtcgtact cggccagcgc gtggcgcacc gccatgagct cgctccgcgc gcggtcgtcg
     6481 ggatcgacgc cgatgtacga cgcccggtcc agcaggatga ccagcgcgcc caccccgcgg
     6541 cgccgcagcg cggccagcgc ccgcacccag gtccgatcgg tggagccggt gacgatgcac
     6601 agggtcatgc cgcgtcgcag ctgggtgagg gtggtgttga gcacctccgc cagcggcgtc
     6661 gagccgtcgg cctggacgtt cgccagcagg tggagcagct tctgctcgac cctggggccg
     6721 cggtcgggct ggtggagctg gaggcgccgc gcggaggcgg tcatggccac cgaccggttg
     6781 tcgctcagcg tccgcatggc gatggaggcg gcgatggtca cggccgtctc gaccgacgca
     6841 tccgtcccga cgcctgcgtg gaccgcgcgg tccaggtcga gcaggatcca caggtcggcc
     6901 gcctgctcga gatcgaactc cttgacgtgc agctccccgt ggcgcgcgct ggacagccag
     6961 tggatgcggt tgatggcgtc gccgtgggtg tacggccgga ccccggccac cagcggcgtc
     7021 gccgcctcgt acctgcgctt ggtgcgcgtg gtgccgtcga tcggtgatgg cggcagacgc
     7081 cagtgcggca gcgggaccac ctcgggaaag acgacgacgc cggtcggctg gccgacggcc
     7141 atctcggcgc tgaagaggcc gaa
//
LOCUS       DASTYP010000240         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_244776, whole genome shotgun sequence.
ACCESSION   DASTYP010000240 DASTYP010000000
VERSION     DASTYP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244776"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..747)
                     /locus_tag="VFV59_10225"
     CDS             complement(178..747)
                     /locus_tag="VFV59_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326246.1"
                     /translation="MRGAAGPILGSAVALLLVACGTAAPTESPIDDVRCPEVPDASAP
                     PNANRDPELAARIPAEIGGQPFPVQTWCASEQSDGLGANTSPDFLATVGVDLTDVTMA
                     AGPGPEIGASAQPPSLTAFRYRGAEEATLRDAFLEVLAESGAAAETGTIGGKEVTVQL
                     GSVIYVQDDTIYLVSGDGPSVEELLNGLP"
     gene            complement(757..2145)
                     /gene="hydA"
                     /locus_tag="VFV59_10230"
     CDS             complement(757..2145)
                     /gene="hydA"
                     /locus_tag="VFV59_10230"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004939424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEU5326247.1"
                     /translation="MRTLIKGGTVVNADATTRADVLVDGERIALIGETLDVEADRTID
                     ASGKWVIPGAIDAHTHMELPFGGTFAKDTFETGTRAAAFGGTTTIVDFAVQSKGASLR
                     EGLDTWMAKAEGKACIDYAFHMIVSDVRDDVLAEMDTLVSEGITSFKLFTAYPGVFFS
                     DDGAIFRAMQQTAKNGGLIMMHAENGLAIDVVAGQTAEAGNTDPYYHGVSRSPVLEGE
                     ATNRVIRLAEVAGVPVYIVHLSAKHALEEVRQARDAGLPAYAETCPQYLFLTLDDMKN
                     GFEGAKFVCSPPLRPGDHADELWKGLAKDDLQVVSTDHCPFDFHGQKELGRGDFRKVP
                     NGLPGVEDRVNLLHSGGVAGGHLTPNRWVEVISTAPARMFGLDSKGAVAVGKDADIVV
                     YDPNAKHTISASTHHMDVDYSCYEGWEVTGRADQVLSRGKVIVDGDEWLGATGDGRFL
                     KRSATGELLART"
     gene            2240..2809
                     /locus_tag="VFV59_10235"
     CDS             2240..2809
                     /locus_tag="VFV59_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEU5326248.1"
                     /translation="MADDRLHYTGDDAADRLLVTDPMALLIGFALDQQVPVQKAFSGP
                     KVLRDRLGTLDAHELATMDTAKLEAAAKGPPAIHRFPSAMAKRVRELAAYVDDTYGGD
                     AARLWTDASDGRDLQRRIAALPGFGEMKVASLSAVLAKRLGVRPKGIDAVLPSHPTLG
                     DVDSPEALAEYQAQKRARKAAARGASGSF"
     gene            2815..3444
                     /locus_tag="VFV59_10240"
     CDS             2815..3444
                     /locus_tag="VFV59_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEU5326249.1"
                     /translation="MAAALLAAAAVVALVDWWAVGTGRRPVELLSKPLVIVLLLGAAA
                     TLTPEADAQRWLVVGALGLSLAGDVALLAPPRWFVVGLAAFLVAHLVYVVALLGEPGG
                     GESPAFGIVLAGIGAAIVGVPIVRAAAARQGAAFAAAVSAYLLAISATVVAAGMSGDA
                     VARIGAVLLYASDGVLGWNRFVRPLPQGRLLTRVPYHAGQALMVLSLLA"
BASE COUNT          434 a         1244 c         1212 g          561 t
ORIGIN      
        1 tgcagacgag ccctggccgc tcgagcaggc ggccagcacg atggccagca gcccgacgag
       61 gaagatcgta attcgcttca cgattgagat tctccgtgtg tacttcggca gcgcgtcgag
      121 tctagccgcc gatgccggaa aacgcgcatg ggccatcggt caggtcgtgg ccgcggttca
      181 gggaaggccg ttgagcagct cctcgaccga tggcccgtcg ccgctgacca ggtagatggt
      241 gtcgtcctgg acgtagatca cacttccgag ctgcacggtc acctccttcc cgccgatcgt
      301 gccggtctcg gcggctgcgc cggactcggc gagcacctcg aggaaggcgt cgcgcagcgt
      361 cgcctcctcc gccccccggt accggaacgc cgtcagggac ggcggctgcg cggatgcgcc
      421 gatctccggc cccgggcccg cggccatagt gacgtcggtg aggtccacgc caacggtcgc
      481 caggaagtcc gggctcgtgt tcgcgcccaa cccgtcggac tgctcgctgg cgcaccacgt
      541 ctggaccggg aagggctggc cgccgatctc cgctgggatc cgagccgcga gctccggatc
      601 gcggttcgcg ttcggcggcg cggacgcgtc cggcacctcg gggcacctga cgtcgtcgat
      661 cgggctttcc gtcggcgccg ccgtgccaca cgcgacgagg agcagtgcga cggcgcttcc
      721 gaggatcggt ccggcagccc cccgcatcgg ctcctcctac gtcctggcga gaagctcgcc
      781 ggtggcgctg cgcttgagga accgcccgtc gcccgtggcg cccagccact cgtcgccgtc
      841 cacgatcacc ttgccgcgcg acagcacctg gtccgcgcgc ccggtgacct cccagccctc
      901 gtagcaggag tagtcgacgt ccatgtgatg ggtcgaggca ctgatcgtgt gcttcgcgtt
      961 cgggtcgtag accacgatgt ccgcgtcctt gccgacggcc accgcgccct tggagtcgag
     1021 cccgaacatg cgcgccggtg cggtgctgat gacctcgacc catcggttcg gcgtcaggtg
     1081 gccgcccgcg acgccgcctg agtgcagcag gttcacccga tcctcgacgc cgggaaggcc
     1141 gttcggcacc ttgcggaagt cgccgcggcc cagctccttc tgcccgtgga agtcaaaggg
     1201 gcagtggtcg gtgctgacaa cctggaggtc gtccttggcg aggcccttcc acagctcgtc
     1261 ggcgtgatcg cccggtcgca gtggtgggga gcagacgaac ttcgcgccct cgaacccgtt
     1321 cttcatgtcg tccagcgtca ggaagaggta ctgcgggcac gtctcggcgt acgcggggag
     1381 gccggcgtca cgcgcctggc gcacctcctc gagcgcgtgc ttcgcggata ggtggacgat
     1441 gtacaccggc acgccggcca cctccgccag gcggatgacc cggttcgtcg cctcgccctc
     1501 caggaccggc gagcggctga cgccgtggta gtacggatcg gtgttgcccg cctcggcggt
     1561 ctggccggcg acgacgtcga tggccaggcc gttctcggcg tgcatcatga tcaggccgcc
     1621 gttcttcgcc gtctgctgca tggcccggaa gatggcgccg tcatcggaga agaagacccc
     1681 ggggtaggcc gtgaacagct tgaagctggt gatcccctcc gagacgagcg tgtccatctc
     1741 ggccagcacg tcgtcgcgga cgtccgagac gatcatgtgg aaggcgtagt cgatgcatgc
     1801 cttcccctcc gccttcgcca tccaggtgtc gaggccctcg cggaggctcg cgcccttcga
     1861 ctgcacggcg aagtcgacga tcgtcgtggt gccgccgaac gccgcggcgc gggtgccggt
     1921 ctcgaacgtg tccttggcga aggtgccgcc gaacggcagc tccatgtgcg tgtgggcgtc
     1981 gatggcgccg gggatgaccc acttgcccga tgcgtcgatg gttcggtccg cctcaacgtc
     2041 cagcgtctcc ccgatcaggg cgatccgctc cccgtcgacg agcacgtcgg cgcgcgtcgt
     2101 ggcgtccgcg ttgaccaccg tcccaccctt gatcagcgtg cgcatcggtt cttcggttcc
     2161 tccgtggcgt gtcgaagtcg agggcgccca tcctaccgcc atggccgccg ccgctggtcc
     2221 ctcgtgctac cgtggcggca tggccgatga tcgcctccac tacaccggcg acgacgccgc
     2281 cgaccggcta ctcgtcaccg atcccatggc gctgctgatc ggcttcgcgc tggaccagca
     2341 ggtgccggtg cagaaggcct tcagcgggcc gaaggtgctc agggaccggc tcgggacgct
     2401 cgacgcgcac gagctggcca ccatggacac ggcgaaactc gaggccgcgg ccaagggacc
     2461 gccggcgatc catcgcttcc cgtccgcgat ggccaagcgc gtccgcgagc tggccgcgta
     2521 cgtggacgac acctacggcg gtgacgcggc ccggctctgg accgatgcgt ccgacgggcg
     2581 cgacctgcag cgacgcatcg ccgcactccc cggcttcggc gagatgaagg tcgcctctct
     2641 cagcgccgtg ctggccaagc ggctcggcgt gcggccgaag ggcatcgacg cggtgctgcc
     2701 gtcgcacccg accctcggcg acgtggactc gcccgaggcg ctggccgagt accaggcgca
     2761 gaagcgcgcc cgcaaggcgg ccgcgcgcgg ggcttcgggc tccttctgac ccccgtggcc
     2821 gcggcccttc tcgcggccgc ggccgtcgtg gcgctggtcg actggtgggc cgtcggcacc
     2881 ggccgccgcc ccgtcgagct cctctccaag ccgctcgtca tcgtcctgct gctcggggcc
     2941 gcggcgacgc tgacgccgga ggcggatgcg caacgctggc tcgtcgtcgg agcgctcggg
     3001 ctgtccctgg cgggcgacgt ggcgctgctc gcgccaccgc gctggttcgt cgtcggtctc
     3061 gccgcgttcc tggtggcgca cctggtgtac gtcgtcgcgc tcctgggcga gccgggcggt
     3121 ggcgagtcgc cggccttcgg gatcgtgctg gccgggatcg gggcggcgat cgtcggcgtg
     3181 cccatcgtcc gtgccgccgc cgcgcgccag ggcgcggcgt tcgcggcagc ggtgagcgcc
     3241 tacctgctcg ccatctcggc gaccgtggtg gcggcgggga tgtcgggcga cgccgtcgcc
     3301 cgcatcggtg cggtgctcct gtatgcctcg gacggcgtgc tgggctggaa ccggttcgtc
     3361 cgccccctgc cccagggacg gctgctcact cgcgtcccct accacgccgg gcaggcgctg
     3421 atggtgctct cgctcctcgc ctagctgtcg g
//
LOCUS       DASTYP010000241         8058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_974594, whole genome shotgun sequence.
ACCESSION   DASTYP010000241 DASTYP010000000
VERSION     DASTYP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974594"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VFV59_10245"
     CDS             complement(<1..1139)
                     /locus_tag="VFV59_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU5326250.1"
                     /translation="MEQPIREIFDQFKQQLPDIDPVETQEWLDSLDSVVEEAGRDRAR
                     FIIYKLLKRARQLQVGLPSLTQTRYINTISPEQEPYFPGDEGMELRIRRMIRWNALAM
                     VIRANSKYEGIGGHLSTYASAASLYEVGFNHFFRGSDDGPGDQIFFQGHAAPGIYARA
                     FLEGRLSEDNLDHFRREALTPELGLSSYPHPRLMPEFWQFPTVSMGLGPMAAIYQARF
                     NRYLNARGVADTSKARVWAFLGDGEMDEPEALGALPVAAREGLDNLTFVVNCNLQRLD
                     GPVRGNGKIIQELEGVFSGAGWNVIKVIWGPEWDELFARDHEGVLRRHLNAIVDGQWQ
                     RMTTASGEYVRENFFGLDPRMLELVKHIPDDELGKLRRGGHSQRKV"
     gene            1499..2869
                     /locus_tag="VFV59_10250"
     CDS             1499..2869
                     /locus_tag="VFV59_10250"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5326251.1"
                     /translation="MRTIYRSEPPWPHRILILGGGFAGVETARHVEALTKRRDDVEVW
                     VVNRENFTLFTPLLPEVCSGQLEARHCVTALRAQLRAPSSWAVTADVQGIDVDGKVVT
                     VLGGDGDTHRLHYDSLVIALGGETATFGIKGIREYAVGMKTLADAFALRNRIIEMLER
                     AELETDPDHRQAHLSFVVGGAGFSGVETAGEVEDFVRRVRRRYYPKIREDELRFHIVE
                     LKDRVLPEMPVEMGEYAATKLRKRGYQIHLGTPMKEVREDGVIVGDGDWIPARTVIWT
                     GGVQPSPVVRDAGIDVDKAGRAIVDPTMQTSRAGVWAIGDCALIPKAEEEGYHAPTAQ
                     NAVREAKRLARNVLATIDNRPDLLEPFRYDVIGTLASIGHHTGVGVVFGIRVRGWLAW
                     FMWRGYYWSRVPGIGGKVRVGFEWLLNALFGSDPVQLKVDYPGEGGMGPSGRRRPTPR
                     ATDPRA"
     gene            2893..3222
                     /locus_tag="VFV59_10255"
     CDS             2893..3222
                     /locus_tag="VFV59_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326252.1"
                     /translation="MSREERRQYQRMMRNMERGPALPPAARARAERNAARRERIRGRG
                     STAGGLTRSFWVRSVAIALILGFVAFSLQWAEGMPFALYVGLAVSAVVLVLAAGFRFL
                     QSRAAAR"
     gene            3293..3646
                     /locus_tag="VFV59_10260"
     CDS             3293..3646
                     /locus_tag="VFV59_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326253.1"
                     /translation="MSEHPHHHGDEPDLPEHEEHGRFSEGQEQEGETLEKEHRGDFAE
                     GLSETHDTVHNPDRGDFARGQEWEHEHAHERGPDYARGLEGEDDTSEGTSEGTSEGTS
                     EGTSEGTSEDAGASF"
     gene            3892..4629
                     /locus_tag="VFV59_10265"
     CDS             3892..4629
                     /locus_tag="VFV59_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEU5326254.1"
                     /translation="MLTDGFSIFIAETVGTGLLILFGDGVVAGVLLARSKAQNAGWIV
                     ITTGWALAVAVGVYAIGSLTGAHLNPAVTIGFAVAGTTDWGLVPWYIAGEMLGAFLGA
                     VLVYFHYLPHWAETEDPGLKLAVFSTGPAIRNTVSNLISEIIGTFALVFGVYAIVANE
                     LAPGFAALLVGFLVWGIGLSLGGPTGYAINPARDLGPRIAHAILPIAGKGSSDWEYSW
                     IPVVGPIIGGILGAVAYQALFTNWLLA"
     gene            4718..5716
                     /gene="dhaK"
                     /locus_tag="VFV59_10270"
     CDS             4718..5716
                     /gene="dhaK"
                     /locus_tag="VFV59_10270"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEU5326255.1"
                     /translation="MKKLINAPEQVVDEALAGMAAAHADLIKVIEPNVVVRVDAPRQG
                     KVGVISGGGSGHEPMHGGFVGMGMLDAACPGAVFTSPVPDQMLAATKAVDGGAGVLHI
                     VKNYTGDVMNFELAAEMAQAEGIEVTSVVIADDVAVQDSLYTAGRRGVGGTVLAEKIV
                     GAAAEEGQDLAAVTALCQRVQDNVRSMGMALTSCTVPHAGKPTFEIGDDEMEIGIGIH
                     GEPGRERRKLTSADEITEALMTPILDDLPFSSGDRVLAFVNGMGGTPLVELYVVYRKV
                     AELCEARGVTIERNLVGNYITSLEMAGCSITLLKLDDEMVRLWDAPVKTPGLRWGV"
     gene            5730..6362
                     /gene="dhaL"
                     /locus_tag="VFV59_10275"
     CDS             5730..6362
                     /gene="dhaL"
                     /locus_tag="VFV59_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEU5326256.1"
                     /translation="MTDFDRAALRRFIDLYAARLHERRDELTDLDSAIGDADHGVNMD
                     RGFTAVTGKLADLDAADLGTVAKTVGMTLISTVGGAAGPLYGTFFLRLATAIGDAEAV
                     DAAGLGAALRAGLEGVVARGKAVLDDKTMVDAMSPAVDAYDAAAGDGIAAALAAAAEA
                     AATGRDRVTPLVARKGRASYLGERSANHQDPGATSTTVLFESLRDAVAEA"
     gene            6405..7892
                     /gene="glpK"
                     /locus_tag="VFV59_10280"
     CDS             6405..7892
                     /gene="glpK"
                     /locus_tag="VFV59_10280"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU5326257.1"
                     /translation="MAKYVLALDQGTTSSRAILFNKDGTIHDVANQEFPQIYPSPGHV
                     EHDPEAIWDSQVAVAKQVIESAGASPEDIAAIGITNQRETTVVWDKNTGKPVQNAIVW
                     QSRITAGYCDELKAAGHEQLFRDKTGLPVDAYFSGTKIRHILKNNPGAQEKAEAGDLL
                     FGTVDSFLIWRLTEGRVHVVDYSNASRTLLFNIHTLDWDDELLGIMGVPRAMMPTPKP
                     SSEVYGETTLFGGSIKIAGDAGDQQAATFGQACFEPGMAKQTYGTGAFMLINTGEEAV
                     PSKNGLLTTIAWGYGGKVIYALEGSVFIAGAAVQWIRDGLRVITNSADVEKLAGKVSD
                     TEGVYMVPAFVGLGAPYWDQYARGAIVGITRGTTIEHIARATVDSMAYQTREVLEARE
                     ADSGVQLSELKVDGGAVVNNDLMQFQADILGVPVERPKVSETTALGAAYLAGLAVGFW
                     ADTNDVVNNWALDRRFEPQMDEEKREKLFRGWKKAVQRALDWEEH"
BASE COUNT         1295 a         2706 c         2860 g         1197 t
ORIGIN      
        1 accttgcgct ggctgtgacc accgcggcgc agcttgccga gctcatcgtc agggatgtgc
       61 ttgacgagct cgagcatgcg cggatcgaga ccgaagaagt tctcgcgcac gtactcgccg
      121 gaggcggtcg tcatgcgctg ccactgaccg tcgacgatgg cgttcaggtg acggcgcagc
      181 acgccctcgt ggtcgcgagc gaagagctcg tcccactccg ggccccagat caccttgatc
      241 acgttccagc cggcgcccga gaagactccc tcgagctcct ggatgatctt accgttgccg
      301 cgcaccggac cgtcgagccg ctgcaggttg cagttgacga cgaaggtcag gttgtcgagc
      361 ccctcgcggg cggcgacggg cagcgcgccg agcgcctccg gctcgtccat ctcgccgtcg
      421 cccaggaagg cccagacccg ggccttgctg gtatcggcga cgccgcgcgc gttcaggtag
      481 cggttgaagc gcgcctggta gatggccgcc atcggcccca ggcccatgct gacggtcggg
      541 aactgccaga actccggcat gaggcgtggg tgcgggtagc tggagaggcc gagctccggg
      601 gtcagcgcct cgcgccggaa gtggtcgagg ttgtcctcgc tcagccggcc ctcgaggaag
      661 gcgcgggcgt agatgccggg agcggcgtgg ccctggaaga agatctggtc gccggggccg
      721 tcatccgagc cgcggaagaa gtggttgaag ccgacctcgt acaggctggc ggcgcttgcg
      781 taggtcgaga ggtgtccgcc gatgccctcg tacttcgagt tggcgcggat caccatggcc
      841 agcgcgttcc agcggatcat gcgccggatc cgcagctcca tcccctcgtc gcccgggaag
      901 tagggctcct gttccgggct gatggtgttg atgtagcgcg tctgggtcag gctgggcagc
      961 ccgacctgga gctgccgcgc gcgctttagc agcttgtaga tgatgaaccg ggcgcggtcc
     1021 cggcccgcct cctcgaccac cgagtccagc gaatcgagcc actcctgggt ctcgacggga
     1081 tcgatgtccg ggagctgctg cttgaactgg tcgaagatct cgcggatcgg ctgctccata
     1141 ggacttccta tggtagccgc cgattcggtg acgtgggatt cggggatccg ggcgcgcccg
     1201 ggagtcgcgg tgagtcgtct gcggtgcgtt ccgcaggacg acgggccgcg tttcgcggat
     1261 ctgccgcata tgaggcagta gccggacgga tcccgagcgc accctcgccg atgagcgtgg
     1321 atcgatcccc gcagcggtgt accgctcgcc tattgcggcg gattcggcgc attccgcgaa
     1381 acgacggcta cggccgatga cgaggtacgc cggtggtggt ccgcatcggc tccgtcacgc
     1441 ctttcggcga ggaaatgtgg cgcgtcctgc ggcacagccc gtgcgatcat cggaggcgat
     1501 gcgcacgatc tatcgatccg agccgccctg gccgcatcgg atcctcatcc tgggcggggg
     1561 attcgcgggg gtcgagacgg cgcgccacgt cgaggcgctg acgaagcggc gggacgacgt
     1621 cgaggtgtgg gtcgtcaacc gcgagaattt cacgctgttc acgccgctgc tgcccgaggt
     1681 ctgctccggg cagctggagg cgcggcattg cgtcaccgcc ctccgcgccc agctgcgcgc
     1741 gccatcgagc tgggcggtca cggccgacgt gcagggcatc gacgtggacg ggaaggtcgt
     1801 caccgtcctg ggtggcgacg gcgacacgca tcggctccat tacgactcgc tggtcatcgc
     1861 cctgggcggc gagaccgcca cgttcgggat caagggcatt cgcgagtacg cggtcggcat
     1921 gaagacactg gccgatgcgt tcgccctgcg caaccggatc atcgagatgc tcgaacgcgc
     1981 cgagctcgag accgatcccg accaccgcca ggcgcacctc agcttcgtcg tcggcggtgc
     2041 cggcttcagc ggcgtcgaga ccgctggcga ggtcgaggac ttcgtgcggc gggtgcgccg
     2101 gcgctactac ccgaagatcc gcgaggacga gctgcgcttc cacatcgtcg agctcaagga
     2161 tcgcgtgctg cccgagatgc cggtcgagat gggggagtac gcggcgacga agctccgcaa
     2221 gcggggctac cagatccacc tcggcacgcc gatgaaggag gtgcgcgagg acggcgtcat
     2281 cgtgggggat ggcgactgga tcccggctcg gaccgtgatc tggaccggcg gggtgcagcc
     2341 gtcccccgtg gtccgggacg ccgggatcga cgtcgacaag gccggtcgcg ccatcgtcga
     2401 cccgaccatg cagaccagcc gcgcgggcgt ctgggccatc ggtgactgcg cgctgatccc
     2461 gaaggccgag gaggagggct accacgcccc gaccgcgcag aacgccgttc gcgaggcgaa
     2521 gcggctggcg cgcaacgtcc tcgccaccat cgacaaccgt ccggacctgc tggagccgtt
     2581 ccgttacgac gtcatcggga ccctggcgag catcgggcat cacacgggtg tcggcgtggt
     2641 cttcggcatc cgggtgcgcg gttggctggc ctggttcatg tggcgcggct actactggag
     2701 ccgcgtgccc ggcatcggcg gcaaggtgcg cgtcgggttc gaatggctgc tgaacgccct
     2761 cttcggatcc gatccggtgc agctcaaggt cgactacccg ggcgaggggg gcatgggacc
     2821 atccggccgt cgtcggccga ctccgcgcgc gaccgatccc cgcgcttgag cgcgccggta
     2881 gactgaacga cgatgtcccg cgaggaacga cgccagtacc agcgcatgat gcgcaacatg
     2941 gagcgcggcc ccgcgctccc tccggccgcc cgtgcccgcg ccgagcgcaa cgcggcccgc
     3001 cgagagcgca tccgcggccg cggctcgacc gccggcggcc tcacgcgcag cttctgggtc
     3061 cgctccgtcg ccatcgcctt gatcctggga ttcgtggcct tcagcctgca gtgggcggaa
     3121 ggcatgccat tcgcgctgta cgtcgggttg gccgtcagcg cggtggtcct cgtgttggca
     3181 gccgggttcc gcttcctgca gtcccgggcc gcggcacggt aaaaacgggc cactcggacc
     3241 tgtctgggtg gcgcaacata cggtcaacct ccagcgctgg gagaggcatc acatgagcga
     3301 gcatccccac catcacggag acgaaccgga ccttcccgag cacgaggagc acggacgctt
     3361 ctcggagggc caggagcaag agggcgagac cctcgagaag gagcaccgcg gcgatttcgc
     3421 cgaggggctg agcgagaccc acgacaccgt gcacaaccct gatcgcggcg acttcgctcg
     3481 cgggcaggag tgggagcacg agcacgctca cgagcgcggc cccgactacg cccgcggcct
     3541 cgagggcgag gacgacacgt cggagggaac ctccgaaggg acgtccgaag ggacgtccga
     3601 gggaacctcg gaaggtacga gcgaagacgc gggcgccagt ttctgacccg gccgtcccat
     3661 ggcgccgggg agagctgagc gcgcatcagg acaggagagg aggtgccccg cgaggagatg
     3721 acgaacgtgg ctccctgggg agcccgtgtc ctcggccggg ccgatgagca gctgatcagt
     3781 cgcccaagac gtggccgccc gtgggcggcg ccacgtccgc aaccctagcc gtgcccaccc
     3841 gagctgcgcg gccggcccta tcggattccc gagtatcgaa gggacaaggg tatgctcact
     3901 gacgggttct caatcttcat cgcggagacc gtcggcacgg gtctcctgat cctgttcggc
     3961 gatggcgtcg tcgccggcgt gctcctggca cgctcgaagg cgcagaacgc cggctggatc
     4021 gtcatcacga ccggatgggc cctggcggtt gccgtcggcg tctatgccat cggcagcctc
     4081 accggcgccc atctcaatcc ggcggtgacc atcggcttcg cggtcgctgg cacgacggac
     4141 tggggcctgg tgccgtggta catcgcgggc gagatgctcg gcgccttcct cggcgcggtg
     4201 ctcgtgtact tccactatct gcctcattgg gccgagacgg aagatcccgg actgaagctg
     4261 gccgtcttct cgaccggtcc cgccatccgg aacacggtgt cgaacctcat cagcgagatc
     4321 atcggcacgt tcgccctggt gttcggcgtc tacgcgatcg ttgcgaacga gctggccccc
     4381 ggcttcgcgg cgctgctcgt cggcttcctg gtgtggggca tcggcctctc gctcggcggc
     4441 ccgaccgggt acgcgatcaa ccccgcccgt gacctgggcc cgcgcatcgc gcacgccatc
     4501 ctgccgatcg ccggcaaggg tagctccgac tgggagtaca gctggatccc ggtcgtcggc
     4561 ccgatcatcg gcggcatcct cggagcggtc gcctaccagg cgctgttcac caactggctg
     4621 ctggcctagc ccagcctgcc gtccggacgc ggcgggacga atcccgccgc gttcgcatcg
     4681 gtcgcgggag ggctgagcga aaggagcgac ggaatcgatg aagaagctga tcaacgcgcc
     4741 cgagcaggtg gtcgacgagg cgctggcggg aatggcggcg gcgcacgcgg acctcatcaa
     4801 ggtcatcgag ccgaacgtcg tggtgcgcgt ggacgcgcct cgccagggca aggtcggcgt
     4861 catctcgggc ggcgggtcgg ggcatgagcc gatgcacggc ggattcgtcg gcatgggcat
     4921 gctcgacgcg gcctgccccg gtgccgtgtt cacctcgccc gtcccggatc agatgctggc
     4981 tgccaccaag gcggtcgatg gtggagccgg cgtgctccac atcgtcaaga actacaccgg
     5041 cgacgtcatg aacttcgagc tggcggccga gatggcccag gccgagggga tcgaggtcac
     5101 cagcgtcgtc atcgccgacg acgtcgccgt ccaggactcc ctctacaccg cggggcgtcg
     5161 cggcgtcggc ggcaccgtgc tggccgagaa gatcgtgggc gctgcggccg aggaggggca
     5221 ggacctggcg gcggtgaccg cgctctgcca gcgggtgcag gacaacgtgc gcagcatggg
     5281 catggcgctc accagctgca ccgtgccgca cgccggcaag ccgacgttcg agatcggtga
     5341 cgacgagatg gagatcggca tcggcatcca cggtgagccg ggtcgcgagc ggcgcaagct
     5401 gaccagcgcg gacgagatca ccgaggcgct gatgacgccg atcctggacg acctgccgtt
     5461 ctcgtcgggc gaccgcgtgc tggccttcgt caacggcatg ggcggcacgc cgctcgtgga
     5521 gctgtacgtg gtctaccgca aggtcgccga gctgtgcgag gcgcgcggcg tcacgatcga
     5581 gcgcaacctc gtcggcaact acatcaccag cctcgagatg gccggctgca gcatcacgct
     5641 gctcaagctg gacgacgaga tggtgcgcct ctgggatgcg ccggtgaaga cgccgggcct
     5701 ccgctggggc gtctgagcga catgcaccga tgaccgactt cgaccgcgcg gcgctccgcc
     5761 gcttcatcga cctgtacgcc gcgcggctgc acgaacgacg cgacgagctg accgacctcg
     5821 actccgccat cggcgacgcc gatcacgggg tcaacatgga ccgcggcttc accgcggtca
     5881 cgggcaagct cgccgacctg gatgccgcgg acctgggcac cgtcgccaag acggtcggga
     5941 tgacgctgat ctcgaccgtt ggcggagcgg cgggtccgct gtacgggacc ttcttcctgc
     6001 ggttggccac ggccatcggc gatgccgagg cggtggacgc cgctgggctc ggggccgcgc
     6061 tccgcgccgg gctggagggc gtcgtcgcgc gcggcaaggc ggtcctcgat gacaaaacca
     6121 tggtcgacgc gatgtcgccc gccgttgacg cgtacgacgc ggcggccggc gacggcatcg
     6181 cggcggccct ggcagcggcc gcggaggcgg ccgccaccgg gcgcgatcgg gtgacgccgc
     6241 tcgtcgcacg aaagggtcgg gccagctacc tgggagagcg gtcagcgaac caccaggacc
     6301 ccggcgcgac ctccaccacg gtcctgttcg aatccctccg cgacgccgtc gcggaggcct
     6361 gaccaaggcc cgagatccac accgaaagga aggacgcgat cgacatggcc aagtacgtcc
     6421 tggcactcga ccaggggacg acctcgagcc gagccatcct gttcaacaag gacggcacga
     6481 tccacgacgt cgcgaaccag gagttcccgc agatctaccc gtcgcccgga cacgtcgagc
     6541 acgaccccga ggcgatctgg gattcgcagg tcgccgtcgc caagcaggtg atcgagagcg
     6601 ccggcgcctc gcccgaggac atcgcggcga tcgggatcac caaccagcgc gagaccaccg
     6661 tcgtctggga caagaacacc ggcaagccgg tccagaacgc gatcgtctgg cagagccgca
     6721 tcaccgccgg ctactgcgac gagctgaagg ccgccgggca cgagcagctg ttccgcgaca
     6781 agacgggcct gccggtcgac gcctacttca gcggcaccaa gatccgccac atcctgaaga
     6841 acaaccccgg ggcgcaggag aaggcggagg ccggtgacct gctgttcggc accgtcgaca
     6901 gcttcctcat ctggcggctg accgagggtc gcgtccacgt ggtggactac tcgaacgcca
     6961 gccggacgct gctgttcaac atccacacgc tcgactggga tgacgagctg ctggggatca
     7021 tgggcgttcc gcgcgccatg atgccgacgc cgaagccctc cagcgaggtc tacggcgaga
     7081 cgaccctctt cggcgggtcg atcaagatcg ccggcgacgc cggtgaccag caggcggcca
     7141 ccttcgggca ggcctgcttt gagccgggca tggccaagca gacctacggc accggcgcct
     7201 tcatgctgat caacaccggc gaggaggcgg ttccgtccaa gaacggcctg ctcaccacga
     7261 tcgcgtgggg ctacggcggc aaggtgatct acgcgctcga gggatcggtg ttcatcgccg
     7321 gcgccgcggt ccagtggatc cgcgacggcc tgcgggtgat caccaactcg gccgacgtcg
     7381 agaagctcgc cggcaaggtg tccgacaccg agggcgtgta catggtgccg gccttcgtgg
     7441 gcctcggcgc gccgtactgg gatcagtacg cgcgcggcgc catcgtgggc atcacccgtg
     7501 gcacgaccat cgagcacatc gccagggcga cggtcgactc gatggcctac cagacccgcg
     7561 aggtgctgga ggcgagggag gccgactccg gcgtccagct ctccgagctg aaggtcgacg
     7621 gcggcgcggt ggtcaacaac gacctgatgc agttccaggc cgacatcctc ggggtgccgg
     7681 tggagcggcc gaaggtctcg gagacgacgg cgctgggtgc cgcctacctg gccggcctgg
     7741 cggtcggctt ctgggcggac accaatgacg tcgtgaacaa ctgggccctc gatcgtcgct
     7801 tcgagcccca gatggacgag gagaagcgcg agaagctgtt ccgcggctgg aagaaggccg
     7861 tgcagcgcgc gctggactgg gaagagcact agtctgtgcc tgaccacgga ccgatcgccc
     7921 ggagccgccc ggctccgggc ctcgatccgt cctctcggag actgtgcgca tgaccgatga
     7981 gacgcccgcc acggtcggca tcgtggtcgt gtcgcactca tcggagctgg ccgaggcgac
     8041 cgtgcagctc gtcgcccg
//
LOCUS       DASTYP010000242         7949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_937354, whole genome shotgun sequence.
ACCESSION   DASTYP010000242 DASTYP010000000
VERSION     DASTYP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937354"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VFV59_10285"
     CDS             <1..910
                     /locus_tag="VFV59_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU5326258.1"
                     /translation="ASSWRKGVTFAIPPTQLIQLEIDEREIGKNYPVAVPLVGDARAG
                     LRDLLDALGAGEPATDYGEEIDRLRTDWDAQIEVKSGSDATPMTMARAVREVQSATRE
                     DAIVVTGAGLPQGMVKQRWVTRQPRTHITSGGFSTMGFTLPAAIGAQLARPDAQVLAI
                     CGDGDFLQTMQELATAAMLGTPVCTVVLDNSGWISIKGGQQAHFGRTAATDFLRDGAP
                     YSPDFAAIGRAFGIHAEAATEPAEVATAVARALATGGPSLVHIPVERDLTVAGPDKTG
                     WWDVPVPEGRPAYDDQAKGRREQQHR"
     gene            907..1812
                     /locus_tag="VFV59_10290"
     CDS             907..1812
                     /locus_tag="VFV59_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU5326259.1"
                     /translation="MSGLTNAPIGCVPIVWNNADLPDLAPETGPDEILDTLARLGFQG
                     CQHGRGFEEGEALRAQLARRGVRLAEWYAPLPATDEGPAPDALEVGRGFLSRLVAGGG
                     EVLVVAIDGSPERNAFSGRVPDGAPRWPDSGFDGLAAILRELADVAPEGVRIAFHPHT
                     ATWVEAPDEVDALAARLRGSRAGLCLDVGHYVVGGGDPVEAIRRHAALISHVHMKDVD
                     PEVLRRLRARELDGFGDAIRARLFTELGRGALDVGGVVEALRDIEYGSWLMVEQDSSW
                     LPPAEAAEVGLRTLRSALTSAGVPR"
     gene            1809..2813
                     /locus_tag="VFV59_10295"
     CDS             1809..2813
                     /locus_tag="VFV59_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5326260.1"
                     /translation="MKVAVLGAGSMGGMHARLLGAMPDVEVLVVDADAGRAAGVATDC
                     GGAPAEWSAALATANAVVIALPPELHAAALEEIVERDLPALCEKPLTEQVGTSRRLVE
                     LVESRAAHVEMGFQRRHDPAFASARASIADGSAGRLHLVRLTALDPRGDHRPPESWPV
                     TEAAPIFLHSSVHDFDVARWLTGAEVVEVTADGSTRDGRRPDDPRGIETATVGMRFSN
                     GTLATLDASWLHPGGYDIRAEILADRLHATVGLGPRTPARHLDWTDAGVPATGSWIGY
                     LQRFEEAYRLELQAFLAAVRGERPPSSTVRDGLEALRVAVAATRSFVERRTVSLDEVA
                     "
     gene            2810..3808
                     /locus_tag="VFV59_10300"
     CDS             2810..3808
                     /locus_tag="VFV59_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5326261.1"
                     /translation="MTATIAEVAARAGVSTATVSRVLSGSAAARPGTRDRVLAAAREL
                     DYRPSAIARALKRRETRTLGLLVTDVGNPFYPQVVAAVEAAAHELGYGIVLANGGNDV
                     TRELQHLELLAERRVDGLIVASAHVTGDHLGRVERARLPVVLVNADVPTAGVASVSTD
                     HRLGARLATAHLVELGHRRIGHISAPTSHAAAQARLEGVRDETPSTNPPVVVEGNADI
                     AGGAAAVAPMLEAGVTAIVAYNDLTAIGALRALRAAGRRVPEQVSVVGFDDIEMAAWT
                     DPPLTTVRQPIADLGRWAVERLVAGMRGAAAEAAAEHARLAPTLVVRASTGPAPPR"
     gene            complement(3805..4650)
                     /locus_tag="VFV59_10305"
     CDS             complement(3805..4650)
                     /locus_tag="VFV59_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEU5326262.1"
                     /translation="MPERPGPLLPLLAVLLVGCALGVPAPSAELPSATPSVSPVASVA
                     SPIPSSTGVASASADASSGASASEAPGTGFDLQVIGCNGGVVLDWTPLAVPAFHHYTA
                     LRSPNREIATEWPPIAPAVDWGDTYITDPYVTSAVDASIIPSATRWSYLVVAYDAEDR
                     PIAASPVRQARMEEVDALDRLLVQPGEDGATTLRWAPFDGFSECFSSYRVLATPAGGA
                     TSVIAVMSNQALTELETDALHAGTTYRLEVQAVRTATQGSFVVGRAAPVTYLVPPVEQ
                     PSASP"
     gene            4756..5166
                     /locus_tag="VFV59_10310"
     CDS             4756..5166
                     /locus_tag="VFV59_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326263.1"
                     /translation="MSDAPAVIAVVDDDAAFGELVLDIVASEGLVGMAVSPRGSVVDR
                     LREVRAALIIMDWRLGEGQEGPTAEPLLRDIADDPELRATPVLVCSADMAALREHVPL
                     LPTELSVTVLEKPFGVEAFIGVVHEALARSGVTR"
     gene            5218..6015
                     /locus_tag="VFV59_10315"
     CDS             5218..6015
                     /locus_tag="VFV59_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEU5326264.1"
                     /translation="MSGVTDLIGWPGVPLLILAALLLAAAGLWLASLRLRDASIVDTF
                     WGPFFLLQAALAAALLDGFEGRRLLLLGLVAVWSVRLATHIASRHAGRGEDERYARWR
                     TQHGDAWPLRSLFQVFVLQAVLAWIIGLPLVAIMGSDAGWGWLDVLGVALWGIGFVFE
                     AVGDVQLTAFIRNPSNRGTTLRTGLWRYTRHPNYFGDAAQWWGFWLLATAAGAWWTIF
                     APAIMTFLLVRVSGVGLLERTIAERREGYAEYMRTTSAFIPLPPKDR"
     gene            6025..6621
                     /locus_tag="VFV59_10320"
     CDS             6025..6621
                     /locus_tag="VFV59_10320"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5326265.1"
                     /translation="MSFSLAPLDVILVRHAEPVPVGTEGVTDDDRPLTDAGRTAAREL
                     AAELDDWEITAIYSSPYERAHETARELAARRGLRVQLLDDLRERRLATEPHDGWRESL
                     EQAWADADFAMPGGESSRDAQRRAVALLDLLRVRHPDGGRLVVASHGNLISLILHALE
                     PGVDFDFHMAMPMPAVYRLTHDGLRWRIMGGHGFAPVD"
     gene            6677..7183
                     /locus_tag="VFV59_10325"
     CDS             6677..7183
                     /locus_tag="VFV59_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326266.1"
                     /translation="MATYQSPLAVIIKGALAGAAGTAVMSAFMERAPELMDRAGMPLP
                     QPPGPPADPDAPSSPTEQLAERVTEGQLPADQRAAAGEAIHWAYGAGWGAYYGVMQST
                     FRLPSVIHGTFLGGLLTFVAVRFVPRLGLTPPPRSREQLVMSTSSHVVYGVTTAIVYQ
                     LLNLGRRG"
     gene            complement(7161..>7949)
                     /locus_tag="VFV59_10330"
     CDS             complement(7161..>7949)
                     /locus_tag="VFV59_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU5326267.1"
                     /translation="RIGLRVNPAVDAHTHPHLATGAASSKFGIALQEVPEAFGRVRRA
                     GRALASIGAHIGSAISDPAPFAALAQRIRGLADQAGVDTIDLGGGLDGAPDAWAQAVR
                     PHLAGRRLIVEPGRSVVADAGWLVTRVVRVQSRGHLVADAGMTDLIRPMLYGARHPVT
                     LLRPGAETPAGRWTLAGPVCEAGDLLADDLDLGGRAGEDALLAIERVGAYGAAMASTY
                     NGRPRAAEAVIEDRRVRLSRRRETLEDLLARDVEASEVSRGARG"
BASE COUNT         1039 a         2909 c         2937 g         1064 t
ORIGIN      
        1 cgcgagctcg tggcggaagg gcgtgacctt cgcgatcccg ccgacgcagc tcatccagct
       61 cgagatcgac gagcgggaga tcggcaagaa ctacccggtc gccgtccctc tggtgggcga
      121 tgcccgcgcg ggcctgcgcg acctgctcga cgccctgggg gccggcgagc cggcgaccga
      181 ctacggcgag gagatcgacc ggctgcgcac cgactgggac gcgcagatcg aggtgaagtc
      241 gggatccgat gcgacgccga tgacgatggc ccgcgccgtc cgcgaggtcc agtccgcgac
      301 gcgcgaggac gcgatcgtgg tgaccggcgc cggcctgccg cagggcatgg tcaagcagcg
      361 ctgggtgacc cgccagcctc ggacccacat cacctcgggc ggcttttcga ccatgggctt
      421 cacgctgcca gcggccatcg gcgcgcagct cgctcgaccg gacgcgcagg tcctcgccat
      481 ctgcggcgat ggcgacttcc tgcagacgat gcaggagctc gcgacggcgg ccatgctcgg
      541 cacgccggtc tgcaccgtcg tgctcgacaa ctcggggtgg atcagcatca agggagggca
      601 gcaggcgcac ttcggtcgca cggcggccac cgacttcttg cgcgacgggg cgccgtactc
      661 gccggacttc gccgccatcg gccgtgcctt cggcatccac gccgaggcgg ccacggagcc
      721 ggccgaggtc gcgaccgcgg tcgcgcgggc cctcgcgacc gggggcccct cgctagtgca
      781 catcccggtg gagcgcgacc tcacggtggc gggacccgac aagaccggct ggtgggacgt
      841 gccggtcccc gagggccgac cggcctacga cgatcaagcc aagggacgac gggagcagca
      901 gcaccgatga gcggactgac caatgcgccg atcggctgcg tgccgatcgt ctggaacaac
      961 gccgatctcc ccgacctcgc gcccgagacc ggtcccgacg agatcctcga cacgttggcg
     1021 cggctcgggt tccagggctg ccagcatgga cgggggttcg aggagggtga ggcgctgcgc
     1081 gcgcagctgg cgcgccgcgg cgtgcggctg gccgagtggt acgcgccgct gcccgccacc
     1141 gacgaaggcc cggcacccga tgccctggag gtcggccgtg gtttcctcag ccgattggtc
     1201 gcgggcggtg gcgaggtgct ggtggtggcc atcgacggct cgccggagcg caacgcgttc
     1261 agcggccgcg tcccggatgg tgcgccgcgc tggccggact cgggcttcga cgggctggcc
     1321 gcgatcctgc gggagctggc ggacgttgcg cccgagggcg tgcgcatcgc cttccacccc
     1381 cacaccgcca cctgggtcga ggccccggac gaggtcgacg cgctggccgc ccgcctccgc
     1441 ggctcccgcg ccggcctgtg cctggacgtc ggccactacg tggtcggcgg cggcgacccg
     1501 gtcgaggcga tccggcgcca cgcagcgctc atctcccacg tgcacatgaa ggatgtcgac
     1561 cccgaggtgc ttcgccgcct gcgggcgcgc gagctggacg gcttcggcga cgccatccgc
     1621 gcccggctct tcacggagct cggccgcggc gccctggacg tgggcggcgt ggtggaggca
     1681 ctccgcgaca tcgagtacgg aagctggctg atggtggagc aggactcgtc gtggctccca
     1741 ccggccgagg cggccgaggt cggactgcgg acgctgcgct cggcgctgac gagcgccggc
     1801 gtcccacgat gaaggtcgcc gttctcggcg ccggcagcat gggcggcatg cacgcccggc
     1861 tgctcggggc catgccggac gtcgaggtcc tcgtcgtcga cgccgatgcg gggcgggcgg
     1921 ccggcgtcgc gacggactgc ggcggggcgc ccgccgagtg gtcggcggcg ctcgcaacgg
     1981 cgaacgcggt ggtcatcgcg ctgccgcccg agctccacgc cgccgccctc gaggagatcg
     2041 tcgagcgcga tctcccggcg ctgtgcgaga agccgctcac cgagcaggtg gggacctcgc
     2101 ggcgcctcgt cgagctggtc gagtcgcgtg ccgcgcatgt cgagatggga ttccagcggc
     2161 gtcacgaccc ggccttcgca tcggcccggg cgtcgatcgc cgacggcagt gccgggcgtc
     2221 tgcacctggt gcggctgacc gccctggacc cgcgcgggga tcatcggcca ccggagagct
     2281 ggccggtgac cgaggcggcg ccgatcttcc tgcactcgtc ggtgcacgac ttcgacgttg
     2341 cgcgctggct caccggggcg gaggtggtcg aggtgaccgc cgacggcagc acgcgcgacg
     2401 gccggcgacc cgacgatcca cgcggcatcg agaccgcgac cgtgggcatg cgcttctcga
     2461 acggcacgct cgcgacgctc gatgcctcgt ggctgcatcc gggcggctac gacatccgag
     2521 ccgagatcct cgccgaccgg ctccacgcca ccgtcgggct cggcccgcgc accccggcgc
     2581 gccacctgga ctggaccgat gccggcgtcc cggccacggg ctcctggatc ggctacctgc
     2641 agcgcttcga ggaggcgtac cgcctcgagc tccaggcgtt cctcgccgcg gtccgcggtg
     2701 agcggccgcc gtcatccacg gtgcgcgacg gcctcgaggc gctgcgcgtg gcggtggccg
     2761 cgacacgctc cttcgtggag cgccggaccg tgagcctcga cgaggtggcg tgacggccac
     2821 catcgccgag gtggcggcgc gcgccggcgt ctccacggcg acggtcagcc gcgtgctctc
     2881 ggggagcgcg gccgcccggc ctgggacccg cgaccgcgtc ctggccgcgg cgcgcgagct
     2941 cgactaccgg ccgtcggcca tcgcccgcgc cctcaagcgg cgggagacgc gcacgctggg
     3001 actcctggtc accgacgtcg gcaacccgtt ctacccgcag gtcgtggccg cggtcgaggc
     3061 ggccgcccac gagctgggct acgggatcgt gctggccaac ggtggcaacg acgtcacgcg
     3121 ggagctgcag cacctcgagt tgctggccga gcgacgggtc gacgggctca tcgtggcgtc
     3181 ggcccacgtg accggggatc acctcggccg cgtggagcgc gcaaggctgc cggtggtgct
     3241 cgtgaacgcc gacgtgccaa cggccggcgt cgcatcggtc agcaccgatc accggctcgg
     3301 cgcacggctc gcgaccgcgc acctggtcga gctggggcac cgccgcatcg gtcacatcag
     3361 cgcaccgacc tcccacgcgg ccgcgcaggc gcgcctcgag ggcgtccgcg acgagacgcc
     3421 ctcgacgaac cctcctgtag tggtcgaggg caacgccgac atcgcggggg gagccgccgc
     3481 ggtggcgccc atgctcgagg cgggcgtcac cgccatcgtg gcctacaacg acctgaccgc
     3541 catcggggcg ctgcgggcgc tgcgggcggc cgggcgccgg gtcccggagc aggtgtcggt
     3601 ggtcggcttc gacgacatcg agatggcggc atggaccgat cctccgctca ccaccgtgcg
     3661 gcagccgatc gccgacctcg ggcgctgggc cgtcgagcgc ctcgtggcgg ggatgcgggg
     3721 cgccgcagcg gaggcggccg ccgagcacgc ccgcctggcg ccgacgctcg tcgtacgggc
     3781 ctcgaccgga cccgcgcctc cccgctaggg cgacgcgctc ggctgctcca ccggcgggac
     3841 gaggtacgtg acgggcgccg cgcggccgac cacgaagctc ccctgcgtgg cggtgcgcac
     3901 cgcctggacc tcgagccggt acgtggtgcc ggcgtgcagc gcatcggtct ccagctcggt
     3961 gagcgcctgg ttcgacatca ccgcgatgac cgaggtcgcc ccgccggccg gcgtggccag
     4021 gacgcggtag ctgctgaagc attcggagaa gccgtcgaag ggcgcccagc gcagggtggt
     4081 cgcgccgtcc tcaccgggct ggaccagcag ccggtcgagt gcgtcgacct cctccatgcg
     4141 tgcctggcga acgggcgagg cggcgatcgg tcggtcctcg gcgtcgtacg cgaccaccag
     4201 gtacgaccag cgcgtcgccg aggggatgat gctggcatcc acggccgagg tcacgtacgg
     4261 atcggtgatg taggtgtcgc cccaatcgac cgccggcgcg atgggtggcc actcggtggc
     4321 gatctcgcgg ttcgggctgc gcagcgccgt gtagtggtgg aacgccggca cggccagggg
     4381 ggtccagtcg agcacgacgc cgccattaca gccgatgacc tggagatcga acccggtccc
     4441 cggagcctca ctggccgatg cgccggacga cgcgtccgcc gacgccgatg ccacacccgt
     4501 cgaggacgga atcggtgagg cgaccgacgc cacgggcgag accgatggcg tggcggacgg
     4561 gagctccgcc gacggcgccg gcacgccgag ggcacacccg acgaggagca cggcgagcag
     4621 gggcagcaga ggaccgggac gctcgggcat cggcgagacg ctaccacctc cgccgagcaa
     4681 gcacgcgcgg cgaaggtgcc atacttgccc gcgttggctg tccgcccgag cggatgatcg
     4741 gcgaacggag gagagatgtc ggacgcaccg gcggtcatcg ccgtggtcga cgacgacgcc
     4801 gcctttggtg agctcgtcct cgacatcgtc gcgtcggagg gtctcgtcgg catggccgtc
     4861 tcgccgcgcg ggagcgtggt ggaccggctg cgcgaggtgc gcgccgcgct gatcatcatg
     4921 gactggcgcc tgggcgaggg acaggagggt ccgacggccg agccgttgct gcgcgatatc
     4981 gcggacgatc ccgagctgcg cgcaacgccg gtcctcgtct gctccgccga catggcggcg
     5041 ctccgcgagc acgtgccgct gcttcccact gagctgtccg tcacggtcct cgagaagccg
     5101 ttcggcgtgg aggcgttcat cggggtcgtc cacgaggccc tcgccaggtc cggcgtcacg
     5161 cgctgacgtc cgggtctgcc gtcgcggtgc gtcatccgag gacgagaatc gcgacgtatg
     5221 agcggtgtga cggacctcat cggctggccg ggcgtgccgc tgctcatcct ggctgcgctg
     5281 ctcctggccg cggccgggct gtggctggcg agcctccgcc tgcgcgacgc cagcatcgtc
     5341 gacaccttct gggggccgtt cttcctgctc caggccgcgc tggctgccgc gctgctcgac
     5401 gggttcgagg ggcgacgtct cctcctcctg ggcctcgtcg ccgtgtggag cgtccggctc
     5461 gccacgcaca tcgcgtcacg ccacgccgga cggggcgagg acgagcgcta cgcccgctgg
     5521 cgcacgcagc acggcgacgc gtggccgttg cgcagcctct tccaggtgtt cgtgctgcag
     5581 gcggtgctgg cctggatcat cggcctgccc ctggtggcga tcatgggcag cgacgccggc
     5641 tggggctggc tcgacgtcct cggggtcgcc ctgtggggca tcggcttcgt gttcgaagcg
     5701 gtcggggacg tgcagctcac cgccttcatc cgcaacccgt ccaacagggg aacgacgctc
     5761 cgcaccgggc tgtggcgcta cacgcgccac ccgaactact tcggtgacgc cgcccagtgg
     5821 tggggcttct ggctcctggc gacggcggcg ggcgcctggt ggacgatctt cgcgccggcg
     5881 atcatgacct tcctcctcgt ccgcgtctcg ggcgtcggac tgctggagcg cacgatcgcc
     5941 gagcgacgcg aggggtacgc ggagtacatg cgcaccacgt cggccttcat cccgctgcca
     6001 ccgaaggacc gatagactcg ccgggtgagc ttctccctgg cgcccctgga cgtcatcctg
     6061 gtccgccacg ccgagcccgt cccggtcggc accgagggcg tcaccgatga cgaccgaccg
     6121 ctgaccgatg cgggccgcac ggcggcccgc gagctcgccg cggagctcga cgactgggag
     6181 atcaccgcca tctactcctc gccctacgag cgcgcgcacg agacggcgcg cgagctggcc
     6241 gcacgccgcg gcctgcgggt gcagctgctg gacgacctgc gcgagcggcg gctggcgacc
     6301 gagccgcatg acggctggcg cgagagcctg gagcaggcct gggccgatgc ggacttcgcc
     6361 atgccgggcg gcgagtcgag ccgcgacgcc cagcgccgcg cggtcgcgct gctggatctg
     6421 ctccgcgttc gacaccccga tggcggacgc ctggtggtcg ccagccacgg caacctgatc
     6481 agcctcatcc tccacgccct cgagccgggc gtggacttcg acttccacat ggccatgccg
     6541 atgccggcgg tgtatcggct cacccacgac ggcctgcgct ggcggatcat gggcggccac
     6601 ggcttcgcgc cggtcgactg acctcccccg gctggcatcg gccttgccct tgcgatcggt
     6661 cgcaaggagg agaccgatgg caacctacca atcgccgctg gccgtgatca tcaagggcgc
     6721 gctggccggc gcagccggga ccgcggtgat gagcgccttc atggaacgcg cgccggagct
     6781 gatggaccgt gccggcatgc cgcttcccca gccgcccggg ccgcctgccg atcccgacgc
     6841 gcccagctca ccgaccgagc agctcgccga gcgcgtcacc gagggccagc tgccggccga
     6901 ccagcgcgcg gccgccggcg aggcgatcca ctgggcgtac ggcgcgggct ggggtgccta
     6961 ctacggcgtc atgcagtcga ccttccgcct gccgtccgtc atccacggca cgttcctggg
     7021 cgggctgctc accttcgtag cggtgcgctt cgtgccgcgc ctgggcctca ccccgccccc
     7081 gcggagccgc gagcaactcg tgatgagcac ctcgtcacac gtcgtctacg gcgtcacgac
     7141 ggcgatcgtc taccagctcc tcaacctcgg gcgccgcggc tgacctcgct ggcctcgacg
     7201 tcccgggcaa gcaggtcctc gagggtttcg cggcggcgac tcagccggac ccggcggtcc
     7261 tcgatcaccg cctccgcggc ccgagggcgg ccgttgtacg tcgaggccat cgcggcgccg
     7321 taggctccga cccgctcgat cgccagcagc gcgtcctccc ctgcgcgtcc tcccaggtcg
     7381 aggtcgtcgg ccagcagatc gccggcctcg cacacgggcc cggccagtgt ccagcggcct
     7441 gcgggcgtct cagcgccggg gcgcaacagc gtgacgggat ggcgggcgcc gtagagcatc
     7501 gggcgaatca ggtccgtcat ccccgcgtcg gccacgagat ggccacggga ctggacgcgc
     7561 acgacgcgcg tgacgagcca gcccgcgtcg gcgaccacgc tgcgacccgg ctccacgatc
     7621 agccgccgcc cggccaggtg cgggcgcact gcctgggccc aggcgtcggg cgcgccgtcg
     7681 aggccgccgc cgaggtcgat cgtgtcgacg ccggcctggt cggccagtcc gcgaatccgc
     7741 tgggccaggg cggcaaaggg cgccgggtcg ctgatcgccg atccgatgtg cgcgccgatc
     7801 gacgcgaggg cacgacccgc tcgacgcacg cgcccgaacg cttcggggac ctcctggagc
     7861 gcgatcccga acttggacga cgccgcgccg gtcgcgaggt gcggatgcgt gtgcgcgtcg
     7921 acggccgggt tcacccgcag gccgatccg
//
LOCUS       DASTYP010000243        42564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_573790, whole genome shotgun sequence.
ACCESSION   DASTYP010000243 DASTYP010000000
VERSION     DASTYP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 42564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42564
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573790"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFV59_10335"
     CDS             complement(<1..558)
                     /locus_tag="VFV59_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5326268.1"
                     /translation="MRLKTLGVLLVTALAAFTMIYWITDEPRRETVATSQEEELLHFG
                     EIIFSNNPAEPAAAGCAQCHGADGTGGEPGAAVIGPNLHSRSLADKLKANPNYVHLAV
                     SYGGVVVSGNVNSQMPAWSSEVGGPLNQQQIDAVVALVESWAAEAANQPPEDVPNTIE
                     AGAEVYASAGCASCHGPDLAGTAAGP"
     gene            complement(562..1221)
                     /locus_tag="VFV59_10340"
     CDS             complement(562..1221)
                     /locus_tag="VFV59_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU5326269.1"
                     /translation="MTTQHASYGAVQGPPTGEITRRNFMRRVLGVGIGLLSLEFLGGT
                     LAFMWPNLTSGLGSDITLGTADDINTAFPEWAEGVPYVYNEANLFFVNVPASKARVEG
                     NGSVASPIPDPGANVDDATAPVDRSVLALWRKCPHLGCQIPQLCDQSEWFECLCHGSK
                     YTVLGEKRDGPAPRGMDRFSVTVQDGAYVVVTREVISGPPVGTVTFDDRAPTDMAHCT
                     G"
     gene            complement(1218..1838)
                     /locus_tag="VFV59_10345"
     CDS             complement(1218..1838)
                     /locus_tag="VFV59_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEU5326270.1"
                     /translation="MTIEKRPNATADKNAPTQAPERAPIAAPAAGGPKLPGPPGVPGT
                     ERFRLLAYVKQESMVRVDKRPDETVLTWPHLLRAEFVMAMLITGLLTVTAVFISAPLE
                     EMANPSVTPHVAKAPWYFLGLQELLSYFNPTVAGVLVIPVYIGGLLIIPFVDRTPYKA
                     ARDRKLMWVFFLCLMIGGLIVTFIGSFFRGPGWNWVWPWEGLFFTL"
     gene            complement(1857..2603)
                     /gene="extP"
                     /locus_tag="VFV59_10350"
     CDS             complement(1857..2603)
                     /gene="extP"
                     /locus_tag="VFV59_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEU5326271.1"
                     /translation="MSNGQPGAGGNAVTRWIKESAVWRSLFRQPYPTTSRTRALAVMN
                     NVFLHLHPVRVKRHAVRYTYTFCLGGISFFLFLALTVTGLYLMFFYVPSVTRAYQDIQ
                     AIEASVAFGAFVRNMHRWAAHLMVLTVFLHMIRVFYHGAYKPPREFNWVVGVLLLFLT
                     LALSFTGYLLPWDQIAFWAITVGTQMAQYAPLLGSESSFMLLGGIQVGQGTLLRFYVA
                     HVIMFPLLAAIFMIVHFWRIRKDGGISGPV"
     gene            complement(2600..4573)
                     /locus_tag="VFV59_10355"
     CDS             complement(2600..4573)
                     /locus_tag="VFV59_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5326272.1"
                     /translation="MISDTAIPLRFQTAEVGVDWLQCNIECQEGCPVNTNCRGYLMLA
                     AEGRFEEGYILARDPNPVAAICGYVCSAPCEKACRRADIDKPLSIRAMKRFLVDWHYG
                     NNMPDNIVVQPPTGRSIGIVGAGPAGLSTAKELASWGHEVHIYEALPSAGGTTLIGVP
                     AFRLPRDVIELDANWVAKHGVQFHYNVEIGRDVTLEQLRDRHDAVVVATGCMYPVAMN
                     VAGEELDGVIYGVDFLKRANLGQPQWVGEHVVIIGGGYTAMDSARTALRLGAKTSTIV
                     YRRGPEEMVVDEEEIHETRFEGVGFEYFASPEEVLADENGHVRGMRFVRTRLGPPDAT
                     GRRSAEPIPGSEFEIACDMVVPCTSQAADNNVLGEYGAPLNRHVGVTDTRTVVDGGSL
                     SSWRTPPMNGSTPKDADGRPMNSLKFDFKRGYVSVNRDTFGTDADGVFAVGDFITGPA
                     TIIEAAGLGRRCARSVDRWLAGLRGEELEELPVINRAVITQSLEHDMPRFYEATRRQE
                     IPMAPPELRRDFTSLVEVGYDTRSAIAEGSRCLQCNHNINIDGPRCILCGLCADVCPE
                     GVIYMIDKAQVGGDDPEQEFFQSWPRGIAMIIDEERCIRCNLCVERCPTNCITMDRLE
                     LERFTPDGKVLLESYKDNVLGQVGQQAGMAVER"
     gene            complement(4903..6942)
                     /locus_tag="VFV59_10360"
     CDS             complement(4903..6942)
                     /locus_tag="VFV59_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5326273.1"
                     /translation="MTRARLAALAGLVLVLAIGLAACSAADPEPTPVPTTYPRAPEEA
                     ASAVVDGYLAAWASADYDAMYASLDAATRARVTLDQFRELHATFADLSLLTSMTTSAG
                     APEVTALPPEARPPEFAAPSPTPVPTTAPSGDPNATPTPQPTPAPSFDPTAPVAGPVP
                     GLAVPVTLDVATDRFGEVDLERRMTLVEDRDGWAIRWSPELLFPELGDAGSFRLDRSI
                     GDRGSILGGDGLVWAQTRDDGIRVYPQEWLAGQVIGYVSEVTAEDLDRLGPASGYRAG
                     DVIGRSGLEAGAEDLLRGIPGWTLVAIGENGVETPVLQTEMVPGADISITIRPTIQAT
                     AQDALAPYGAAATAVIDPNSGDVWALASTPAFNPNAMTLGTTLDGQPLNAAGQGQIFN
                     KAVLGAYPAGSSFKPFTLAVALKTGVVTPQSLVTCPPTWQYGSFTFRNYEEHSLPGLV
                     SLAQAMAFSCNTTYMPLALSVYQADETALTDLLKEFGFGQASGIGYLVEETGIVPDDQ
                     WLSENGRGGYSGFEQVQLAIGQGAFLGTPLQLANAYATIGNGGTLWRPRILTTATLPD
                     GTVVESVEPEPIRQVSVSREDLAYVTDTLQAVVTLPYGTGTAAFAGFGVPVAGKSGTA
                     ETGTPDPHAWFPAYAPADAPTISVATILTYIPLGTGGSDAAPLVRRVMVAHFAGG"
     gene            complement(6939..7658)
                     /locus_tag="VFV59_10365"
     CDS             complement(6939..7658)
                     /locus_tag="VFV59_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEU5326274.1"
                     /translation="MPDDKLPTPERVLVVAAHPDDIEFGAAGTVARWVAEGARVRYLL
                     VTRGDKGTDDPAADLARIARLREEEQRAAAAEIGVEGVDFLDEPDGQVEPSLRLRERL
                     TYAIRAFQPEIVMTHDPTVLFVNNEWVNHPDHRAVGMTTVDAVFPTARDPLNFHEHLQ
                     AGLGPWKVAELYLWSTNEANQLVDIGATLDAKIAALARHASQFHAFDEIARWVRRRNE
                     ELGERAGYRAAEGFRRVTLAR"
     gene            complement(7699..10683)
                     /locus_tag="VFV59_10370"
     CDS             complement(7699..10683)
                     /locus_tag="VFV59_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5326275.1"
                     /translation="MRIERLELDGFGRFHDAHWDLGDGLTVLLGANEAGKTTLLNAVR
                     ALLFGFESSRDGRAWYPALAGGRRGGRLQLRTASGERWTVERHGERGGGGALAVRAPN
                     GNQGGQETLDRLLHGADKDLFNNIFAFGLGELQTLASLSADAVRGRIYGAGAGLGGTS
                     AIDLERRLRQQLDAVFLPQGSKRPLNQLLARAEELRREIADLAKQPQEHAEASRERDE
                     LRARADALRDELRTLRERLARLAALRRAAPLAVELGQLEAELQAGDPTLDAFPDDGLV
                     VLERRTADLEQARAVLATLDEQLDTACRRRAALTVDEPILAVADELVTLSAEATRRTA
                     ADGRARDLDAALQRHGAAIAEQLARVGGWDEATLVALDDSIGAVEATREHERALSAAR
                     TALGEAEQRRRAAAEELEVRLREVGGGVEIGDDEALGARSQLVAAFDDLRERRAAAAA
                     VAAHGRAAAFRTGALAGMAALVGAAVFLAAWLAGAPLPGIALGGATAALTMAVLRRLV
                     ARTSVPADPTALDREREDLLRRLGLPLDALDGAVRSLAEELAQQRARHARVREQSSAL
                     DARRADVERRLHDVEAAREQAATAEQAWATWLADHGLPAAQTPEAVRHVLAAAGIARR
                     AAAERDAALQELETLRSEERDFASRADELIVRVTGARPADGVRRHATIVTLAQRLDQA
                     RADARHAAELDAGIAQATERRQVAAAAVEEREAALRAHLEATGCLDPDGLRRRATEAQ
                     TRRRLRETIRARRNELAVLAGNDEAVPELVAEARASDGASLEAAEAEARDALERLEAQ
                     EREANSRIGALDARMRQLEAAEELGARRQELAIVEGRAAAMARAWAVRAVALRLLEET
                     RRRYERERQPDVVRSAEAHFERITGGRYPRIIAPPGDASVRVEMVGGTMRATDELSRG
                     TSEQLYLALRFGLIEQFAIHAEPLPVVMDDILVNFDAERAARAAAAIRDLADRHQVLY
                     FTCHAWTAEVLDPGGARTLALG"
     gene            complement(10680..12008)
                     /locus_tag="VFV59_10375"
     CDS             complement(10680..12008)
                     /locus_tag="VFV59_10375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEU5326276.1"
                     /translation="MSSIEPFRFIHAGDLHLDSPVEGLSSEAPPEVLTLLRGATTEAW
                     RAIVRTAIAERVDLVVIAGDVFEVSSPSLLGQTRFRDGLAELAAAGIPSFVVHGNHDP
                     LDGRSWAPSLRFPDMVHRFGTRAVEAVPVLRDGREIARVHGRSYPRPAVTENYAAGFR
                     AEPGAPFSIGLLHTNVGDRPGHHNYAPCSVEDLRAAGMDYWALGHIHQPGRVLDDPPA
                     YYSGIPQGRDPGELGARGCWLVEVDAARRVTPRFVVTDVVRWQPLDVDTGDLADDEAL
                     RRACVSAMAAALDAADGRSLIVRLRLTGRGPLHANLGRAGYVDDLRLLLNEEAGATPP
                     FAWVESLTDATRPDVDVTARREAPDFVGDFLRTVAAARRSGTTTDPEEHERWLGELRS
                     ALAPVFDDSPRGRRHLAEGRPDDAVLVGELLDEAETLGLDLLLAAEEDRG"
     gene            complement(12148..12294)
                     /locus_tag="VFV59_10380"
     CDS             complement(12148..12294)
                     /locus_tag="VFV59_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEU5326277.1"
                     /translation="MLWTIIVILLVLWLLGLIGNIGGGLIWLLLVIAAIVLVFQLLSG
                     RRTV"
     gene            complement(12375..12524)
                     /locus_tag="VFV59_10385"
     CDS             complement(12375..12524)
                     /locus_tag="VFV59_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326278.1"
                     /translation="MIWVLTAAALLLWLAGVILQLGQWVNLFLLAAAVLLVYQVIEER
                     RPHGG"
     gene            complement(12628..13092)
                     /gene="bcp"
                     /locus_tag="VFV59_10390"
     CDS             complement(12628..13092)
                     /gene="bcp"
                     /locus_tag="VFV59_10390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEU5326279.1"
                     /translation="MSRLTAGDRAPDFSLTADDGSTVSSESLAGSRYVLYFYPKDDTA
                     GCTAQACGLRDRWTDVSATGVAIYGVSPDNAKSHGKFRAKYDLPFPLLVDEEHRLADA
                     YGVWIEKNFMGRNYFGNERTTFVVGADGRIEHVLPQVKPTEHTDQLLEVLAA"
     gene            complement(13104..14069)
                     /locus_tag="VFV59_10395"
     CDS             complement(13104..14069)
                     /locus_tag="VFV59_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5326280.1"
                     /translation="MPRSDDLRFRAGSLMLAGTLTLPDGVEGRAPWALLVGSWLPRDR
                     DGAWDRAGHPGWFARPATDDPHLLARLADALAARGVASLRYDPRGCGESEGDWAATDL
                     FTRIDDARDALGAMRSRRDLDLRRTAIVGHGEGVAIALSVAIADPAVGAVGLVGASAR
                     SFRDVLRRGVATRSRTGTDLRHPIVVALDRRSEELIERAQRREPSMTIDVDGPVQLML
                     APYEQAFHTPPLALGTMLRRNVVLAHGSEDAWAAPDETDLLADALRAGGNDPLVQRIP
                     GAGHDLAEVDDGAIGAFADALVSRMEPRELPPVLVAIEEIGDAGG"
     gene            14096..15418
                     /locus_tag="VFV59_10400"
     CDS             14096..15418
                     /locus_tag="VFV59_10400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5326281.1"
                     /translation="MTPPLPTLEPSFAPAPEPVPFTPDPRTALPPAAEVVVVGAGMVG
                     ASAAYHLARAGMRPLVIEANAPAWGASGRNAGMALAGLGGHFPRVTRLVQEAGGRSIL
                     DYTMRSLDMLEVWDHELGGHGIEWDRFGSLDLAVTEAEEAHIRHMAGIQAAEGLEVEV
                     IGADALRELAPGLAVEEVRAAKWTPRDAKLNPFRLCYALLEDAMAHGATVATGVRVDA
                     LRAHAGRMAGVETSHGPVDAGAVLVATNAYTPALVPSIAANLTPIRETVCVTEMLPFS
                     IGRPGFETNQCNEYWRQMRTGEVVIGGFAVADEAMGIGSYSITVRPQVPPLLAGLLAR
                     LHPALTDARIVRCWAGLLDFASLEMPMAGALPASDGTAVPGGYVAAGLTGHGHPYAPI
                     LGLLLAELITEGEARTLSLAPFDPRRYVGERHEPTWLEPFRGETPIER"
     gene            complement(15436..16239)
                     /locus_tag="VFV59_10405"
     CDS             complement(15436..16239)
                     /locus_tag="VFV59_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEU5326282.1"
                     /translation="MQLLTPLVPDPAAPLARANPVAKLAAALVLLVALFASLDHVTAL
                     VILAGLAIALGMSGLSPSALASRAWLVALAAVSIAVFNVVFAAEQVGPTAVSLGPLRI
                     GAGTLVDGLGLALRLLAIAGAGILATATTQPTELAHALVQQLRVSPRFAIGALAAVRL
                     LPLLAQEWQTLSMARRARGVDSGRNPVAAVRLFAGQLMALLVGAIRRGTRMAAAMEAR
                     GFGAQDCRTVARPQRMTAGDWGWIAAAAALAAVAIGVSLALGTWRPLIG"
     gene            complement(16239..17705)
                     /locus_tag="VFV59_10410"
     CDS             complement(16239..17705)
                     /locus_tag="VFV59_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5326283.1"
                     /translation="MTDGPTGAAGFEARGWGLRYPGRRAWAVRGLDLVVEPGERVLLL
                     GPSGAGKSTLLAALAGLIDPAGGAETEGALLVDGRDPGDARDRTGLLFQDPEAQIVMG
                     RAGDDVAFGLENRCVPTPEIWPRVDAALAAVDFPYGRDRPTHALSGGEQQRLALAGTL
                     ALRPGLMMLDEPTANLDPDGAAEVRRAVARILERTGVTLLLVEHRLDDVLPLVDRVVV
                     LQAGGGVVADGPPVEVFRAEGSRLAEAGVWVPDHPPAPPVRREAPGPEPLVIAERAMF
                     RYPGADRDALRPTDVQLRAGEVLGVVGPNGSGKSTLAMLLAGLLRPSAGSVRATEALA
                     PGRGMEPLADWPARELARHVGTVFQDPEHQFLTGSVREELLLGPGRIGVSEAAAGRRA
                     DELLERLHLAALARANPYTLSGGEKRRLSVATALATAPAALVLDEPTFGQDRRTWAEL
                     LHLLAGLRDEGRGICVVSHDPPFIRALADRVITLEAPG"
     gene            complement(17731..18324)
                     /locus_tag="VFV59_10415"
     CDS             complement(17731..18324)
                     /locus_tag="VFV59_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU5326284.1"
                     /translation="MTTTTSSAGGWRIVDIVVGAVVAVAFGVVFLAWNAIYTATDPIF
                     AALKPAQAILYGVWLVPGVLVGLIVRRPGAAFFGGFVSAAVSVLLGSPWGVDVLISGA
                     IQGGAAELAFALGMYRNWNLPFAMLAGALAGIGAAIHDIAVYYPDLGVDFWVIFAVAT
                     VISGILIAGIGSWYLLRALVATGVLAEFAVGREQREV"
     gene            complement(18321..18974)
                     /locus_tag="VFV59_10420"
     CDS             complement(18321..18974)
                     /locus_tag="VFV59_10420"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU5326285.1"
                     /translation="MRVVVVASGAIEPADDAWLDSAELVIAADGGASSLARMARVPDR
                     LVGDLDSTDAELVERLRARGVGIERHPADKDASDTELALEAAIASGATELILLGALGG
                     QRLDHELANLLLLADPAFAKVDLRLVRGTTTVRVLHGDSVLALEGAPGAIVSLLPLAA
                     DAIGVTTDGLRWPLADATLRAGRSRGLSNEVVAAPASVRLREGTLLVVESSTEGANP"
     regulatory      complement(19055..19162)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            19204..19704
                     /locus_tag="VFV59_10425"
     CDS             19204..19704
                     /locus_tag="VFV59_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU5326286.1"
                     /translation="MQPTDGSAHPNVERVVAAARNAGVDIAVQRFPEGTRTAQDAAAA
                     VGCEVGQIVKSLVFVADGRPVVALVSGTNRLDTTRLASTLGATAVRRADGDEARAATG
                     FAIGGVPPFGHSQPLDVVMDHDLATWERVWAAAGLPDAVFDISPGELERASGARLADI
                     AERPGG"
     gene            19725..20474
                     /locus_tag="VFV59_10430"
     CDS             19725..20474
                     /locus_tag="VFV59_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326287.1"
                     /translation="MPLGTVFAVLLAAGLLLVAGCAALIRIGGAQASLARRLAGPAEV
                     KVGRLLDADELPQRPVRVVGRIRCRDPLNAGDGERLVAYHRDVEVRIGGRWRSIERLR
                     ETRSFDLWDHDGSVPVDPARAAEPLITIPKVWRGSPAELEEPHASAVARLAERHGPAT
                     EARAVTRTVNVTDRLLVIARPMRDDAGRLALEPPEGGFLVSTLDLPDAMRLLGGRHNR
                     LSALAVAGMGVGIAVAAIGLVGLVAASLIGA"
     gene            20606..23977
                     /locus_tag="VFV59_10435"
     CDS             20606..23977
                     /locus_tag="VFV59_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5326288.1"
                     /translation="MPSDQTDPGDSLLSVAQAAAVLGVHPNTIRTWTDAGRLTAYRIN
                     ARGDRRVRRRDVERLLVEDGIAAMEPTAPAIAVDGAVHDRVTATIARLAQKLAASQSP
                     AGLARATVEAVRADLGARAAAVYLAADDGFTLAAHAGFDAPPAPHRSLDGAPPPHEPT
                     AGLLEAPITGSRSVLGLVIADAGGSRDVELLLRAVAATAGATLQHLRLLGRARRQLQR
                     SRALRSVTKDLTGTLDLNSVLGDAVELTRSLFEAEKAGLWLVNDAKHPFRLAAQHGLS
                     PAFLGHVADLRLSDETLGVRAAREGRSFWLSNVDTDAGAGSMREIYAAEGIVTACLVP
                     LVGRDETIGVIALYHTSSRIWSDDSLELVQAFADQAAVAIQNARLYRSVADQAARMRS
                     IQDLSARLNRLTDVRAIAQAIVAEAKTLAEYHDIRVYRVDWETGWCEPIAFTREMLVG
                     DPADAESLLRVEIGQGFTGWVAENAEPLLINDAVDDPRGQTIEGTDDVPESMLVVPMI
                     YEGRTTGVIVLSQLGYERFSADDLQTMTIFAGYAAQAMANASAYEQLRDQSAELARRA
                     DSQRRLLEINERLLSTLDQAGVLETIADGLRDVVRYDNLSIYRTDPQQQAMLPVLTRE
                     RHAEQVSRYIIPFGRGLMGWAVQHAQPILANDALSDPRALQIPGTPEDPEAVVVVPLI
                     ADGEVLGALNVSRVGGPEVHFSESDFELVQLFAAQASIALRNADTHHAVSRLAETDAL
                     TGLGNHGAFQRDLGRLIEARGTAATRGGGLTLLMMDLDSFKAFNDVHGHPAGDALLHR
                     VAMAIYGAARSEDRVYRYGGDEFALILPATTAAHGARIGERIRTAVAALTAHDPTPVT
                     ITVGVATLTPDVADRAALVAAADTALYYGKRAGEDRIVRFDRMPPDVGDLRGTLDDLA
                     AAALRDGDDARVVEHLVERASQLGAAAHHDAAASVRDALLTVARAVDGRGRTNRGHAD
                     RVGRLAGALAMTLHLDEDVRRTVELAGRLHALDDRGVAEISSVPSLHDVSALIVGARR
                     LADEGARRARRTSRMRGRLGAHIVAVANGYDELVSGVGGPRVGRREALARLASHPSSY
                     RDEVLSALAGIVGERRDSGRRRRVSDSAGEVPGAA"
     gene            24009..24791
                     /locus_tag="VFV59_10440"
     CDS             24009..24791
                     /locus_tag="VFV59_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326289.1"
                     /translation="MTHPRLALPRTSGAVGDGRARTRRLGAGLLILGTLLAACSTAEV
                     QPTPTGTAAPTSATASASPTPTATPSPTPEPTPRYTNEPDEDLAALIPTEAAGVPVVV
                     APFDEFALTPGDVAIAYGELGNRFASLVVAYVEQPRTTLYAMRLDGEPAVTEDLELFL
                     ATAGRYVGIAGLDPDPWTLDDSAGYRVWVRPEDNATAAGTMVYTWAAEDYVFLLIGVD
                     DTVNRAIIEQLPTEPAPTPRPTASPSASAGSSAPASASPSGG"
     gene            24796..26130
                     /locus_tag="VFV59_10445"
     CDS             24796..26130
                     /locus_tag="VFV59_10445"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEU5326290.1"
                     /translation="MRIRELAERVRHSLWFIPALAVVIAVVVALGMLALSDRLDASGA
                     HLPLIFGAGPDGARAMLGAIGGSVITVAGTIFSITIVALQLTSSQFSPRVLRNFLRDR
                     PNQLVLGVFIGTFTYCLLVLRSIRAEATDRAAFVPNVAVTGALLLAFLSIGMLIYFIH
                     HVSVRIQVTSILASVASDTLHTIETVADWLEDRPERAWRSAGLLDRVPPAPPTGAIPA
                     DRARVRIAAHHSGYLQFLDIGRLVRAAAEAEGRLEVLVAPGGWVQRDAPVATFAGPDD
                     ADTERLGDAVNGALSLAAERSTQQDVAFGIQQIVDIGAKALSPGVNDPTTAINALDRL
                     LQILVAAGVAQDPPQAFADADGVTRLDVPMPGLHELVELALDQLRHYGASTPAVALHM
                     ARVLSQLRDAVGERAAPAIRRQADLLVEAAARIPLASERAAIMAAVAPLRSA"
     gene            26190..27269
                     /gene="cax"
                     /locus_tag="VFV59_10450"
     CDS             26190..27269
                     /gene="cax"
                     /locus_tag="VFV59_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEU5326291.1"
                     /translation="MFAKVAYGLAIGGTILTLALRVFGADETLTFGVSAITILGLAYV
                     LGHATEQLGMATGPRIGGIMNATVGNIGEIIIAAFLILDDKIEIVHASITGSIVGNLL
                     LVLGASLLVGGLKNGVQEYDEQLTGMNAASLILATIGLIVPATFAFVIGGGEGTAPGD
                     AEFFQIEALTVGVAVLLILTYAAQTWFFLRAPEAPAAARHEEEEVPEWSWKVALGVLG
                     ASAAALTFASEVLVQTLEPAVASLGISEFFIGIILIPLIGNLAEHVVGVTLAYKNKMD
                     FSLITSIGSATQIALFAVPVLIFFGLIVGHPVTLVFTPLEVVAVAVGVFIAGFIALDG
                     RSNWVEGLQLISVYSILAIAFFFLT"
     gene            27319..28170
                     /locus_tag="VFV59_10455"
     CDS             27319..28170
                     /locus_tag="VFV59_10455"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5326292.1"
                     /translation="MLTLPGPVVDVDWLAAHRADPSLRVADVRWSLAGPAGRELYARG
                     HVPGAVFLDAETELSSPGEGPGRHPVPAADRLARVLGQRGIGDEHVVVAYDDAGGSIA
                     ARLWWLFRHYGHDGQAAVLDGGIGAWTSTGLPLETDPPAYPPATWTVRHVADDLVGTD
                     EVVGMLDGETLLLDARARERYRGETEPIDPRAGHIPGAVSAPHADNLGADGRFLPADA
                     LRRRFRALGVADDRPVVAYCGSSLTATHDLLALELAGLPGGRLYEGSWSHWSSDPSRP
                     AVTGAAP"
     gene            28167..29204
                     /locus_tag="VFV59_10460"
     CDS             28167..29204
                     /locus_tag="VFV59_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEU5326293.1"
                     /translation="MTGSHDEWDGWDEGAAEDRIGRKRDRLARLLSVASILYSRGSGE
                     NGVAVSEIAKLTGMTTRTVYRDINALDEELGVPVFQAGRGRYGIDRKFFLPPLRLSVP
                     EAIVLFLASRLIARWSDQYDASVVSAFTKLADTLPQPIARHVAASMLTIGAADPNEPF
                     SRSFSAVARGWAEGRVVEIDYDPGTAPAKRTRVHPYFLEPDAALRSVYLIAYDEPAGG
                     MRTYKVERIRSATLTQDRYEIPEDFDPDAWLANSWGIWSPDSTPPVHVRLRFESEVAH
                     RVREAVWHRSQELTELPEGRVELAVTVNGIVEIRPWILSWGESVEVLEPPELREAIAA
                     SVRGAAARYAD"
     gene            29194..29841
                     /locus_tag="VFV59_10465"
     CDS             29194..29841
                     /locus_tag="VFV59_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU5326294.1"
                     /translation="MPTEVEARFAADGPLPLEALTRAARLGPAVLGRPRTADETDRYL
                     DTDDGALATARWACRLRTRDGLTRISLKGPPEAGDRDDWHHRRPELEGPATEELDPSA
                     WPPSSARDLLDRLRGGAPLRERLRLVQRRTERSVTLDGIPFGTLTCDQVRVEREGSAA
                     GDLHVVELELHDEGGADARAALETLAGALDFMPGLRPEPRTKLERALDLLGESRP"
     gene            29838..32078
                     /locus_tag="VFV59_10470"
     CDS             29838..32078
                     /locus_tag="VFV59_10470"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU5326295.1"
                     /translation="MTAVASWVRRRTEAEWVALAAAAATFGYVGWDGALWDARFQLLL
                     HLLAIGAIAALGVLAWKGGSLPRTRIDAPLLALVAAFALATTSALNVGMSLRAMGAIV
                     ATALMLPVALVALRHRASWVGLIVAVPVVVLSVPALVVLLGRRLEWVLAGAPGLPPIR
                     LPGEGTPFGSVAVPPFVIWPAWAIAGAIEDDRLRWWVRAALVAVGVPLTILSGSRSAW
                     LAIGVTLLVAGLPWAWQRRHRLDLKRGWTPRRIAVAAGGLVVVAGLAAVLLPRVTAVT
                     SLLYRASLWRDTLRAWSTDPLFGIGPGFMPYARQAAAPDFSFPVRQPHSHNLPLGVLG
                     DAGLVGLAAAIVVVVALALVAGPWRSRTPVGRAASLTLLGLGIGGLFEDLTFLPNFNL
                     LAVLLVALALTDAGAVDWVRLPRPPLRRVALGLGTLAVAAPLVTAMVIADAGAVAYRA
                     GVDTAASARWTDATRWFGRATELDPWHPANPKALAVAADGAGETALARRAAEDATERN
                     PGDAPSWVNLALLCRELADETCQSRATEQAVATAGFLRPELVNAAFSYESLGFTDEAD
                     DAYRRSLLSQLLTAFATDWPRPIGIGDATIEDQAGAQFELNRLVAWWAMDEPIRPELV
                     TDPATRALAHAMRGERDQAEEWLSVAIDDHPDQIPTWDVALVLRRHWGEPIDDVLRIE
                     EAVRGRPLTERDAPPPDPNASPGLTFDIGSFRAYPRDGLIGGAIRLVTRPPYPWILEV
                     LLPPSG"
     gene            complement(32117..32740)
                     /locus_tag="VFV59_10475"
     CDS             complement(32117..32740)
                     /locus_tag="VFV59_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326296.1"
                     /translation="MQPTEGASPFDDPGYLFEPWWPGVRAQAWVENRTLMRLRAEGLA
                     DALDAFAELGDELPERLIEDGVVLDGWLLTLDDGGWLDTSLLHRRLTGDRRAGRPAYV
                     ATDLLWTDGRSLQRRRFSARRQHLEAVLLDGDRCVVSHAVRGEGTLLAEALGRFGLVS
                     ISARRLDARYRAGPAGDAWQRLPITPAAVPQRPRLSLIQRLPFPELG"
     gene            complement(32827..33684)
                     /locus_tag="VFV59_10480"
     CDS             complement(32827..33684)
                     /locus_tag="VFV59_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU5326297.1"
                     /translation="MARSMWRGAIQFGLVTIPVKLYLATEQGGIGFNLLHASCLNRIQ
                     MKVYCPYHDEVVPRSETVRGYEWSKGKYVVVTEEDIDSVPLKTMRAIEIEMFVDASRD
                     NAGTQFVKQAYYLEPEQIGAKAFYLLKSVLAEQNKSAICKIVLKDREQLAALNPFSST
                     MLLTTLHWPDEVRAIDELNLPEDQIEIKPAEKKMAEQLVASMTGDFSADEYHDDYRQA
                     LMAVIERKVAGEKPEPAQRTEPTNITDLMAALEASVAAARSERRAAVEEPAAEATAKA
                     PRQRRRKTA"
     gene            33872..34261
                     /locus_tag="VFV59_10485"
     CDS             33872..34261
                     /locus_tag="VFV59_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5326298.1"
                     /translation="MNDDTALRAYVLEDDPDIAALEADLLRDMGFAVVTCTRIVELGD
                     LMELALPHLLVVDVMLPDGDGGDITELLRSAWPGIPVVIVSAASRERLAELAPIGPVV
                     AKPFDLESFRNAVGEAIEGSRELRSVS"
     gene            complement(34274..34753)
                     /locus_tag="VFV59_10490"
     CDS             complement(34274..34753)
                     /locus_tag="VFV59_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5326299.1"
                     /translation="MSESIHAIVDPTGCRTEARTPKGQPITLDADPPEGRDSAAGPKE
                     TVLAALAGCAAADVASILRKKRQSAATYEIDVRGESAGEHPRVFTAITIEHQVTGEVE
                     PEALRRSIELSATRYCPVNAMLSASVEIEHRYRLTAADGSEHAALVARTGPSGTRVE"
     gene            34783..35541
                     /locus_tag="VFV59_10495"
     CDS             34783..35541
                     /locus_tag="VFV59_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326300.1"
                     /translation="MRVIRPSALAGNGRRRTLLLSATMLAVGVLAAQLTLLTPASTAT
                     GVLAASAALGLGVGTAWLLRALRPNRARSAAEALAGLLAPTLDDSYTLLLAPTLPVRD
                     AARLDGVLVGPAGVRVLTVRDWDGRYRVRGRGWEFDARGRRGWITCRTNPSFEAASLS
                     EGFSRWASDAGLVLPDVALRPAIAFPNRRSRIVLEEPIDEVITTDNAPWWANTIGRVQ
                     RLDPSSVARVVAAVMEAAEARSAVGRRPAAQDAG"
     gene            complement(35543..36172)
                     /locus_tag="VFV59_10500"
     CDS             complement(35543..36172)
                     /locus_tag="VFV59_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326301.1"
                     /translation="MSGFGFEGFGTVHDVAIEAYTSAYRVSGHMKTRFTRVADILNQN
                     TGAHLVIDEATIGEHGDPAPPQRVPQALVPLNEVLVVLLTDQPGEGAVQGSGDHMRIP
                     KRGVRAQLGLPPFRLTGTIYVPQGSRPMDGLLNVPDRFLPMTDAAITSAAHPQLNRES
                     AVVALARDRAQVLIVADDERPDELLAEVLDEATAEAWLRSGQGHPGAEG"
     gene            complement(36225..37973)
                     /locus_tag="VFV59_10505"
     CDS             complement(36225..37973)
                     /locus_tag="VFV59_10505"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU5326302.1"
                     /translation="MPPRAASTPFARLAATADAVGATRSKNAKRDLLAGYLRDLPAGD
                     LVAATTFFAGRPLTDPTAKLGMGWVQQGAALAAASGADEERLRTAYLRHSDFGDAAAE
                     LLGERAPSGGAPLTVADVADGFASIAAAGSAEERVTRFTDLFRRATGDEARFIGRISS
                     RETRIGLREGLLEEAVAAAFDRDLDAVRRAHMLTGDIGETALLARDDRLAGAALHVGR
                     PIRFMLASPVADAAEVMRRVGDEAWIEDKYDGIRAQLHLEADGTVRLFSRDLNDVTRS
                     FPEIAQAGAALTGDGPLVLDGELVPWRAGSVLDFASLQTRLGRVRPSQALLEEVPVVL
                     VAFDLLHHGGRDLLEVPLRERRGELERLGLPEVTGERVLLSHLATAASAKEVDRHFDD
                     ARERHNEGLMVKDPASVYQPGRRGLGWLKLKKALATLDCVVVGVEWGHGKRRGVLSDY
                     TFAVRETDAPDARLLTIGKAYTGLTDAEIAEMTEHFKAITLRDFGRYRTVVPEVVVEI
                     AFDRIMRSGRHRSGFAMRFPRIVRIRDDKTPADIDTLATVEALFGEQASGRILLATGA
                     RGETEEIAATGEAPPE"
     gene            38034..38648
                     /locus_tag="VFV59_10510"
     CDS             38034..38648
                     /locus_tag="VFV59_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEU5326303.1"
                     /translation="MLPRMVFPWQKRNVPDELAARVPPGQVLTEKFPVLHFGRVPDYG
                     DLSGWDFRVWGEVDEPFRLNWAEFRNLPVAELTTDIHCVTRWSKLDTRWEGVAFSHVA
                     EVAVVRPSARFVIFHAEGGFTANVPLETAMRPECILAWSYDGQPLTAEHGYPLRAIVP
                     GTYFWKSAKWLRGVEFTTDDRLGFWERNGYHNDADPWKEQRYSR"
     gene            complement(38636..38953)
                     /locus_tag="VFV59_10515"
     CDS             complement(38636..38953)
                     /locus_tag="VFV59_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326304.1"
                     /translation="MAKRGSAEQNGSNTVNTGGTAAAGKAGGNMSVREAGRKGGKTTR
                     ARYGPEFFQEIGAKGGKAVSERYSNEHFREIGRKGGRRVAELIARAKTMPGEPGEDSG
                     QRE"
     gene            39091..39402
                     /locus_tag="VFV59_10520"
     CDS             39091..39402
                     /locus_tag="VFV59_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326305.1"
                     /translation="MHPMLRNTIIGIVGLIIAGALVALALLGDDSDLSVLAMLVAGIV
                     AVAIGLFVYAQGWVWGSRAARQRATGQSLLIAVGGGIMVLIAGVAMAGLVILVLLFYL
                     G"
     gene            39465..39541
                     /locus_tag="VFV59_10525"
     tRNA            39465..39541
                     /locus_tag="VFV59_10525"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39499..39501,aa:Glu,seq:ttc)
     gene            complement(39546..39725)
                     /locus_tag="VFV59_10530"
     CDS             complement(39546..39725)
                     /locus_tag="VFV59_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326306.1"
                     /translation="MGRQNVPRDQWLDLGSECPRCGEQITEANVYSWRPLPEDPKLLL
                     LWCPNCGDRVEINHI"
     gene            complement(39725..40168)
                     /locus_tag="VFV59_10535"
     CDS             complement(39725..40168)
                     /locus_tag="VFV59_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326307.1"
                     /translation="MFKSLVRTFKGPSRDPRMAYISRPLPTSLPDEQVVEALDLAFAE
                     NPNPAYLVETLPPALRHVTGRDFQVLDRSVRDVTGAFTKTMVMIRDGDVGSWPGYAAM
                     SYPLIRPSAKAEETRAAIVAADSPDGSEPSGPKPGWAPPPPPERR"
     gene            40383..41249
                     /gene="galU"
                     /locus_tag="VFV59_10540"
     CDS             40383..41249
                     /gene="galU"
                     /locus_tag="VFV59_10540"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU5326308.1"
                     /translation="MRKAVLPAAGFGTRFLPASKAVPKEMMPLVDRPIIQYAVEEAVA
                     SGIEQMIIVIATGRSAIEDHFDSNSGLERWLEDRGDIEMLRQIRRISEFGTVAFVHQR
                     EQLGLGHAVLMAKDLVGDEPFAVLLSDDVMLNPGGEPVTRQLIDAYLAHRGSVIAVQR
                     VDRQDVSRYGIVDPLHEEGRLVEIRNLVEKPTVEEAPSELAVLGRYILTPKIFDVLEH
                     TTRGAGGEIQLTDAIRTLIGEQPVFGYQFEGTRHDAGTRLGWLKANVAVALESDEADE
                     FRTYVRGLDLRD"
     gene            41260..>42564
                     /locus_tag="VFV59_10545"
     CDS             41260..>42564
                     /locus_tag="VFV59_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5326309.1"
                     /translation="MIGRLISGRYRLIAPLGEGGMATIWRAVDEQLDREVAVKILRPQ
                     FSADPGFAARFKQEARAAGGLSHPNIVSVYDYGTDGADGEQYIVMELVEGRDLASIIS
                     ERGALSVDDSVRVAIAVASALEAAHRKGIVHRDVKPGNILITDHGDVKVTDFGIARAV
                     SEASMTVTGTTLGSVHYFSPEQARGDEVTGASDVYALGIVLFEMLTGRRPFEGDSAAA
                     VALKRLNEDAPTPSGIGRPVPAGLEAILARALAREPIDRFPDAGALAEALRVWRRDPD
                     AVPGVVPASASTMAGAAAAAPVPAGTPPAGEPTVYVPPRVARPADRVPVAPPPRRADV
                     YQRPRDEGTPWWMWLLAVLAVFLLGAIGFLAVQLLGSGTPAATPSPTAATVIVPTCDD
                     VSLATLRQQADELGVTVDTESEPSEDIDEGRVIRCDPETGQPV"
BASE COUNT         5649 a        15648 c        15370 g         5897 t
ORIGIN      
        1 cggccccgcg gccgtcccgg ccaggtcggg accgtggcac gacgcgcagc cggcgctcgc
       61 gtacacctcg gcgcccgcct cgatcgtgtt cgggacgtcc tccggcggct ggttggccgc
      121 ctccgccgcc caggactcga ccagcgcgac gacggcgtcg atctgctgct ggttgagcgg
      181 tccgcccact tccgagctcc aggccggcat ctgcgagttg acgttgcccg acaccaccac
      241 gccgccgtag ctcacggcca ggtgcacgta gttcgggttc gccttcagct tgtccgccag
      301 gctgcgggaa tgcaggttcg gaccgatgac ggccgcgccc ggctcgccac ccgtgccgtc
      361 cgcgccgtgg cactgcgcgc agcccgcggc cgccggctcg gccgggttgt tggagaagat
      421 gatctcgccg aagtggagga gctcctcctc ctggctggtg gcaaccgtct cacgacgcgg
      481 ctcgtcggtg atccagtaga tcatcgtgaa ggcggcgagg gcagtgacga gcagcacccc
      541 cagcgtcttg agtcgcatcg gctaccccgt gcagtgcgcc atgtcggtcg gcgctcgatc
      601 gtcgaaggtc acggtgccga ccggtgggcc ggagatgacc tcgcgggtga cgaccacgta
      661 ggcgccgtcc tggacggtga ccgagaagcg gtccatgccg cgcggtgcgg gaccgtcgcg
      721 cttctccccc aagaccgtgt acttcgagcc gtggcagagg cactcgaacc actcgctctg
      781 gtcgcagagc tgcgggatct ggcagccgag gtgcgggcac ttgcgccaca gggccaggac
      841 cgatcggtcc accggcgcgg tggcgtcgtc cacgttggcg cccggatccg ggatcggcga
      901 cgcgaccgag ccgttgccct cgacccgtgc cttggacgcc ggcacgttga cgaagaacag
      961 gttggcctcg ttgtagacgt acggcacgcc ctcggcccac tccgggaagg cggtgttgat
     1021 gtcgtcggcc gtgccgagcg tgatgtcgga ccccaggccc gaggtcaggt tcggccacat
     1081 gaacgccagc gtgccgccca ggaactccag gctgagcaga ccgatgccca cgcccagcac
     1141 gcgccgcatg aaattgcgcc gcgtgatctc gccggtcggc gggccctgga ccgcgccgta
     1201 ggaagcgtgc tgcgtcgtca tagcgtgaag aagaggccct cccacggcca gacccagttc
     1261 cacccgggac cgcggaagaa ggagccgatg aaggtcacga tcaggccgcc gatcatgagg
     1321 cacaggaaga agacccacat cagcttgcga tcacgcgccg ccttgtacgg ggtccggtcc
     1381 acgaacggga tgatcagcag gccgccgatg tagacgggga taaccagcac gccggccacc
     1441 gtcgggttga agtagctcag cagctcctgc agcccgagga agtaccacgg cgccttcgcg
     1501 acgtggggcg tcaccgacgg gttcgccatc tcctccagcg gggcgctgat gaacacggcc
     1561 gtgaccgtga gcaggcccgt gatgagcatg gccatcacga actcggcgcg cagcaggtgc
     1621 ggccaggtca ggacggtctc gtccggccgc ttgtccaccc ggaccatcga ctcctgcttg
     1681 acgtaggcga ggagccggaa gcgctcggtg cccgggacgc ccggcgggcc ggggagcttc
     1741 gggccgcctg ccgccggggc ggcgatcggc gcgcgctcgg gcgcctgggt cggggcgttc
     1801 ttatcggccg tggcgttcgg ccgcttctcg atcgtcatcg gtcagcgcac cttaccctac
     1861 accggaccgg agatgccgcc gtccttccgg atcctccaga agtggacgat catgaagatg
     1921 gcggccagca gcgggaacat gatcacgtgc gcgacgtaga agcgcagcag cgtcccctgc
     1981 ccgacctgga tcccgcccag gagcatgaag ctcgactccg agccgagcag tggcgcgtac
     2041 tgcgccatct gcgtcccgac cgtgatggcc cagaacgcga tctggtccca gggcagcagg
     2101 tagccggtga agctcagcgc cagcgtcagg aacagcagca gcacgccgac gacccagttg
     2161 aactcgcgcg gcggcttgta ggcgccgtgg tagaagaccc ggatcatgtg caggaagacg
     2221 gtcagcacca tcaggtgcgc cgcccaccgg tgcatgttgc gcacgaaggc gccgaaggcg
     2281 acggatgcct cgatggcctg gatgtcctgg taggcgcgcg tcacgctcgg cacgtagaag
     2341 aacatcaggt acaggccggt gacggtcagc gccaggaaga ggaagaagct gatcccgccc
     2401 aggcagaacg tgtacgtgta gcgcaccgcg tggcgcttga cccgcaccgg atgcaggtgc
     2461 aggaacacgt tgttcatcac ggccagcgcg cgggtgcggc tggtggtcgg gtacggctgg
     2521 cggaagaggc tgcgccagac ggccgactcc ttgatccagc gagtcacggc gttgccgccg
     2581 gcaccgggct gtccgttgct catcgctcca ccgccatgcc ggcctgctgc cccacctgcc
     2641 ccagcacgtt gtccttgtag ctctccaaca gaaccttccc gtcaggcgtg aaccgctcga
     2701 gctcgagccg atccatcgtg atgcagttgg tcggacaccg ctcgacgcag aggttgcagc
     2761 ggatgcagcg ctcctcgtcg atgatcatgg cgatcccgcg cggccacgac tggaagaact
     2821 cctgctccgg gtcgtcgcca ccgacctgcg ccttgtcgat catgtagatc acgccctcgg
     2881 ggcacacgtc ggcgcagagt ccgcagagga tacaacgggg accgtcgatg ttgatgttgt
     2941 ggttgcactg gaggcagcgc gagccctccg cgatggcgct gcgcgtgtcg tagcccacct
     3001 cgaccaggct ggtgaagtcg cggcgcagct ccggcggcgc catcgggatc tcctggcgcc
     3061 gcgtcgcctc gtagaagcgc ggcatgtcgt gctcgaggct ctgggtgatg accgcgcggt
     3121 tgatgaccgg cagctcctcg agctcctcgc cgcgcagccc ggccagccag cgatcgacgc
     3181 ttcgggcgca tcggcggccc aggccggcgg cctcgatgat cgtggccggc ccggtgatga
     3241 agtcgccgac cgcgaagacg ccatcggcgt cggtgccgaa ggtgtcgcgg ttcacgctga
     3301 cgtagccgcg cttgaagtcg aacttgagcg agttcatggg gcggccgtcg gcatccttgg
     3361 gggtcgagcc gttcatcggc ggcgtgcgcc aactggagag gctgccgccg tcgaccaccg
     3421 tgcgcgtgtc cgtcacgccg acgtggcggt tgagcggggc gccgtactcg ccgagcacgt
     3481 tgttgtccgc tgcctggctg gtgcagggca cgaccatgtc gcacgcgatc tcgaactcgc
     3541 tgcccgggat cggctccgcg ctgcggcggc cggtggcgtc cggcggcccg agacgggtgc
     3601 ggacgaagcg catgccgcgc acgtgaccgt tctcgtcagc cagcacctcc tcgggggagg
     3661 cgaagtactc gaagccgacg ccctcgaagc gcgtctcgtg gatctcctcc tcatccacga
     3721 ccatctcctc ggggccgcgg cggtagacga tggtgctcgt cttggcgccc aggcggaggg
     3781 ccgtgcgggc ggagtccatg gcggtgtagc cgccgccgat gatgacgacg tgctcgccga
     3841 cccactgcgg ctgccccagg ttggcgcgct tgaggaagtc gacgccgtag atcacgccgt
     3901 cgagctcctc gccggccacg ttcatggcga cggggtacat gcagccggtg gcgaccacga
     3961 ccgcgtcgtg ccggtcacgg agctgctcca gggtgacgtc gcggccgatc tcgacgttgt
     4021 agtggaactg cacgccatgc ttcgcgaccc agttggcgtc gagctcgatg acgtcacgcg
     4081 gcaggcggaa ggccgggacg ccgatgaggg tcgtgccccc cgccgaggga agcgcctcgt
     4141 agatgtggac ctcgtggccc cagctggcaa gctccttggc ggtcgagagg cccgcgggac
     4201 cggccccgac gatgccgatg ctccgacccg tcggcggctg caccacgatg ttgtccggca
     4261 tgttgttgcc gtagtgccag tcgaccagga agcgcttcat ggcgcggatg gacagcggct
     4321 tgtcgatgtc ggcccggcgg caggccttct cgcacggcgc gctgcacacg tagccgcaga
     4381 tcgcggcgac ggggttcggg tcgcgggcca ggatgtagcc ctcctcgaac cgcccctccg
     4441 cggccagcat caggtagccc cggcagttcg tgttcaccgg gcagccttcc tggcactcga
     4501 tgttgcactg gagccagtcg acgccgactt cggcggtctg gaacctgagg ggtatcgcgg
     4561 tatcgctgat cacgacaggt ggagactctc ccgcggcgtg gttcgaccaa gggcttgaag
     4621 gagcggcccg ccgatgctac ccgctccgcg accggccgcg caaggcgagc gatgggcgag
     4681 gcgagcgcgc aggaacggtt cgcgcgtctc gacgttgcgc gattctgtca tccacgcctg
     4741 ggggtggtga cgggccgggt cgcggcgcgc gacgttgcca aggcacgcca gccaggcgtg
     4801 aacgcggccc ggatggcgga gtcgagcggt ctcggcgcgc ccatcaccac ggggaggcgt
     4861 gaccggcaat cggggcgacc gaggcggccg ggcgcacccc ggtcatccac cggcgaagtg
     4921 ggccaccatc acccgccgga cgagcggggc ggcgtcggag ccgccggtgc cgagcgggat
     4981 gtaggtcagg atcgtcgcga cgctgatggt cggcgcatcg gccggggcgt aggcggggaa
     5041 ccaggcgtgc ggatcggggg tgccggtctc ggccgtgccg gacttgccgg cgacggggac
     5101 gccgaagccg gcgaacgccg ccgtgccggt gccgtacggg agggtgacca cggcctggag
     5161 cgtatcggtc acgtaggcga ggtcctcgcg ggagacgctg acctggcgga tcggctccgg
     5221 ctcgacggac tccacgacgg tcccgtccgg cagggtggcc gtagtcagaa tccgcggtcg
     5281 ccacagggtg ccgccgttgc cgatggtggc gtaggcgttc gccagctgca gcggtgttcc
     5341 caggaacgcg ccctggccga tggccagctg gacctgctcg aagccggagt agccgccgcg
     5401 gccgttctcg gacagccact ggtcgtccgg cacgatcccc gtctcctcga ccaggtagcc
     5461 gatgccgctt gcctggccga acccgaactc cttgagcaga tcggtgagcg ccgtctcatc
     5521 ggcctggtag accgacagcg ccaggggcat gtaggtggtg ttgcagctga aggccatggc
     5581 ctgcgccagg ctgacgaggc cgggcagcga gtgctcctcg tagttgcgga aggtgaagct
     5641 gccgtactgc caggtcggcg ggcaggtcac cagcgactgg ggcgtgacga cgccggtctt
     5701 gagcgcgacg gcgagcgtga acggcttgaa cgaggagccg gccgggtagg cgccgagcac
     5761 cgccttgttg aagatctgtc cctggccggc ggcattcagc ggctgcccgt cgagggtggt
     5821 gcccagcgtc atggcgttcg ggttgaaggc cggcgtgctg gccagggccc acacgtcgcc
     5881 ggagttcgga tcgatgaccg cggtcgcggc ggctccgtac ggggcgagcg catcctgggc
     5941 ggtggcctgg atggtcggcc ggatggtgat ggagatgtcg gcgcccggga ccatctcggt
     6001 ctggagcacg ggcgtctcga cgccgttctc gccgatggcc accagcgtcc agcccggaat
     6061 gccgcggagg aggtcctcgg ccccggcctc caggccgctt cgaccgatga cgtccccggc
     6121 gcggtacccg ctggcaggac ccagccgatc gaggtcctcg gccgtcacct cgctgacgta
     6181 gccgatgacc tggccggcca gccactcctg cgggtacacg cggatgccgt cgtcgcgcgt
     6241 ctgggcccag accaggccgt ccccaccgag gatgctgccg cggtcgccga tcgaccggtc
     6301 aagccggaag ctgccggcgt cgccgagctc ggggaagagc agctcgggcg accagcggat
     6361 cgcccatccg tcgcggtcct cgacgagcgt catccgccgc tcgaggtcga cctcgccgaa
     6421 ccggtcggtc gcgacgtcga gcgtgaccgg gaccgccagg ccggggacgg ggccggcgac
     6481 gggggcggtc gggtcgaagg agggtgccgg cgtcggctgc ggcgtgggcg tggcgttcgg
     6541 gtcgcccgat ggcgcggtgg tcggcacggg agtgggcgag ggggcggcga actccggggg
     6601 acgggcctcc ggcggcaggg cggtgacttc cggagcgcct gccgaggtcg tcatgctggt
     6661 gagcagcgac aggtccgcga aggtcgcgtg cagctcgcgg aactggtcga gcgtcacgcg
     6721 tgcgcgcgtc gcggcatcga gcgacgcgta catcgcgtcg tagtccgccg acgcccacgc
     6781 cgcaaggtac ccgtcgacca ccgcggacgc cgcttcctcc ggcgcgcgcg gataggtcgt
     6841 gggcaccggc gtcggctcgg gatcggcggc ggagcatgcg gcgagcccga tggcgaggac
     6901 gaggacgagg ccggcgagtg cggcgagccg cgcgcgcgtc atcgggccag ggtcacccgc
     6961 cggaaacctt ccgccgcccg gtagccggcc cgctcaccga gctcctcgtt ccggcggcgc
     7021 acccagcggg cgatctcgtc gaaggcgtgg aactggctcg cgtgacgggc cagcgccgcg
     7081 atcttggcat ccagcgtggc gccgatgtcg accagctggt tcgcctcgtt ggtgctccac
     7141 aggtacagct ccgccacctt ccatgggccc aggcccgcct ggaggtgctc gtggaagttc
     7201 agcgggtcac gcgcggtcgg gaagacggcg tcgaccgtgg tcatgcccac cgcgcggtgg
     7261 tcggggtggt tgacccactc gttgttgacg aagaggacgg tcggatcgtg ggtcatgacg
     7321 atctcgggct ggaaggcccg gatggcgtac gtcagccgtt cccgcagcct gagcgacggc
     7381 tccacctggc cgtccggctc gtcgaggaag tccacgccct cgacgccgat ctccgcggcg
     7441 gcggcccgct gctcctcctc gcggagccgg gcgatgcgcg ccaggtccgc ggccggatcg
     7501 tcggtgccct tgtcgccgcg cgtgaccagc aggtagcgga cgcgggcgcc ctcggccacc
     7561 cagcgcgcca cggtgcccgc ggcgccgaac tcgatgtcgt cggggtgcgc ggcgacgacg
     7621 aggacgcgtt cgggggtggg cagcttgtcg tctggcatgg gctgcggccg atcctagcag
     7681 tcagccgggc ggcagccgtc agcccagcgc cagggtccgg gcgccgcccg gatccagcac
     7741 ctcggccgtc cacgcatggc aggtgaagta gaggacctgg tggcggtcgg ccaggtcgcg
     7801 gatggcggcg gccgcgcgcg ctgcgcgttc ggcgtcgaag ttgaccagga tgtcgtccat
     7861 gaccaccggg agcggctcgg cgtggatggc gaactgctcg atgaggccga agcgcagcgc
     7921 caggtacagc tgctcgctcg tcccgcgcga cagctcgtcc gttgcgcgca tggtgccgcc
     7981 gaccatctcg acgcggacgc tggcgtcacc gggcggggcg atgattcgcg ggtatcggcc
     8041 gccggtgatc cgctcgaaat gcgcttcggc ggaccgcacc acgtccggct ggcgctcgcg
     8101 ttcgtagcgt ctcctcgtct cctcgagcag tcgcagggcg actgcgcgga ctgcccaagc
     8161 gcgagccatg gcggccgccc gcccctcgac gatggcgagc tcctgccgcc gcgctccgag
     8221 ctcctcggcc gcctcgagct gccgcatgcg ggcgtcgaga gccccgatcc ggctgttcgc
     8281 ctcgcgctcc tgcgcctcga ggcgttccag cgcgtcacgc gcctccgcct cggccgcctc
     8341 cagactggcg ccgtccgagg cgcgcgcctc ggccacgagc tccggtacgg cttcgtcgtt
     8401 gcccgcgagg accgcgagct cgttgcgccg cgcccggatg gtctcgcgca ggcggcggcg
     8461 ggtctgggct tccgtcgcgc gccggcggag gccgtcggga tcgaggcagc cggtcgcctc
     8521 gaggtgggcg cgcagcgcgg cctctcgctc ctcgactgcc gccgccgcaa cctggcgtcg
     8581 ctcggtggcc tgagcgatcc cggcgtccag ctccgcggcg tggcgcgcgt ccgcgcgcgc
     8641 ctggtccagc cgctgcgcga gggtgacgat ggtggcgtgg cgccgcacgc catcggccgg
     8701 ccgcgcgccg gtgacgcgca cgatcagctc gtccgctcgg gacgcgaagt cgcgctcctc
     8761 ggaccggagc gtctcgagct cctgcagggc ggcgtcccgc tcggccgcgg cgcgtcgcgc
     8821 gatgccggcg gctgccagca cgtggcggac cgcctcgggc gtctgcgccg ccggcaggcc
     8881 gtggtccgcc agccacgtcg cccaggcttg ctccgccgtc gcggcctgct cgcgcgccgc
     8941 ctccacgtcg tggagccgcc gctcgacgtc cgcgcggcgc gcgtcgaggg cgctcgactg
     9001 ctcccgaacg cgcgcgtgtc gggcgcgctg ctgcgcgagc tcctcggcca gcgatcgcac
     9061 ggcgccgtcg agtgcgtcga gcggcaggcc cagccggcgg aggaggtcct cgcgctcgcg
     9121 atccagcgcc gtgggatcgg ccggcacgga agtgcgggcg acgagccgac gcaggacggc
     9181 catggtcagc gccgcggtcg ctcccccgag cgcgatcccg ggaagcggtg ccccggccag
     9241 ccaggccgcc aggaagaccg ccgcgccgac cagggccgcc atgccggcca gggcgccggt
     9301 gcggaatgcg gcggcccgac cgtgcgcggc gaccgcagcg gcggcggcgc gccgctcgcg
     9361 gagatcgtcg aaggcggcga ccagctgcga tcgtgcgccc agggcctcgt cgtcgccgat
     9421 ctcgacaccc ccgccgacct ctcgcaggcg cacctccagc tcctcggccg cggcgcggcg
     9481 gcgctgttcc gcctcaccga gcgcggtgcg cgccgcggac agcgcccgtt cgtgctcgcg
     9541 ggtcgcctcc accgcaccga tggagtcgtc gagggcgacg agggtcgcct cgtcccaacc
     9601 gccaacgcgg gccagctgct ccgcgatcgc tgcgccgtga cgctggagcg cggcgtccag
     9661 gtcgcgggcc cggccgtcgg ccgcggtacg ccgcgtggcc tcggcgctga gcgtgacgag
     9721 ctcgtcggcg acggcgagga tcggctcgtc gaccgtcaac gccgcgcgcc gccggcaggc
     9781 ggtgtcgagc tgctcgtcca gcgtggccag caccgcccgc gcctgctcca ggtccgcggt
     9841 gcggcgctcg aggaccacga ggccgtcatc cgggaaggcg tcgagcgtcg ggtcgccggc
     9901 ctgcagctcc gcctcgagct ggccgagctc cacggccagc ggcgcggctc gacgcagcgc
     9961 cgccaggcgc gccagccgct cgcggagcgt gcggagctcg tcgcgcagcg catcggcccg
    10021 ggcacgcagc tcgtcacgct cgcggctcgc ctcggcgtgc tcctggggct gcttcgccag
    10081 gtcggcgatc tcccggcgca gctcctcggc gcgcgcgagc agctggttga gcggccgctt
    10141 gctgccctgc ggcaggaaga cggcgtccag ctgctgccgc agccgtcgct cgagatcgat
    10201 ggcactggtg ccgccgaggc cggcgcccgc gccgtagatg cggccgcgga ccgcatcggc
    10261 ggagaggctg gccagggtct ggagctcgcc cagcccgaag gcgaagatgt tgttgaagag
    10321 gtccttgtcg gccccgtgca gcaggcggtc cagcgtctcc tggccgccct ggttgccgtt
    10381 cggcgccctg accgccaggg ccccgcctcc tccgcgctcg ccgtggcgct ccaccgtcca
    10441 gcgttcgccg gacgccgtcc gcagttggag gcgaccgcca cggcggccac cggccagcgc
    10501 cgggtaccag gcgcggccgt cgcgggacga ttcgaagccg aagagcaggg cccggacggc
    10561 gttcagcagc gtcgtcttgc ccgcctcgtt cgcgccgagc agcaccgtca ggccgtcacc
    10621 caggtcccag tgggcgtcgt ggaagcggcc gaagccgtcg agctccaggc gctcgatcct
    10681 cacccccggt cctcctcggc ggccagcagc aggtcgaggc caagcgtctc cgcctcgtcc
    10741 agcagctcgc cgaccagcac ggcgtcgtcg gggcggccct ccgccaggtg gcggcgaccg
    10801 cggggtgaat cgtcgaagac cggcgcgagc gccgaccgca gctcgcccag ccagcgctcg
    10861 tgctcctccg gatccgtcgt cgtgccgctg cgccgggcgg cggccaccgt ccgcaggaag
    10921 tcgccgacga agtccggcgc ctcgcgccgg gcggtgacgt cgacgtcggg acgggtcgca
    10981 tcggtcagcg actcgaccca ggcgaacggc ggggtcgcgc ccgcctcctc gttgagcagg
    11041 aggcgaaggt cgtccacgta gcccgcgcgc cccaggttcg cgtggagcgg gccgcggccg
    11101 gtgaggcgca gacggacaat gagcgagcgg ccatccgccg catccagcgc agccgccatg
    11161 gcgctcacac acgcgcggcg cagcgcctcg tcatcggcga ggtcgccggt gtccacgtcg
    11221 agcggctgcc agcgcacgac gtcggtgacc acgaagcgcg gcgtgacgcg gcgggcggcg
    11281 tcgacctcca ccagccagca gccccgcgcg ccgagctcgc cggggtcgcg gccctgaggg
    11341 atgcccgagt agtaggcggg cggatcgtcg aggacgcggc ccggctggtg gatgtggccc
    11401 agcgcccagt agtccatgcc ggcggcccga agatcctcga ccgagcacgg cgcgtagttg
    11461 tggtgcccgg gccggtcgcc gacgttcgtg tgcagcagac cgatcgagaa cggcgcaccc
    11521 ggctccgcgc ggaagcccgc ggcgtagttc tccgtgacgg cgggccgcgg gtacgaccgg
    11581 ccgtggacgc gcgcgatctc gcgcccgtcg cgcaggaccg ggacggcctc cacggcgcgg
    11641 gtgccgaagc ggtggaccat atccgggaag cggagcgacg gcgcccagct gcgaccgtcg
    11701 agcggatcgt ggttgccgtg gaccacgaag ctcgggatgc ccgcggccgc cagctcggcc
    11761 aggccgtcgc ggaagcgggt ctggccaagc agtgatgggc tcgagacctc gaacacgtcc
    11821 ccggcgatca cgacgaggtc cacccgctcg gcgatggccg tgcgaacgat ggcgcgccag
    11881 gcctcggtgg tcgcgccacg cagcaaggtc aggacctcgg gcggcgcctc cgagctcagt
    11941 ccctcgacgg ggctgtcgag gtggaggtca ccggcatgga tgaagcggaa gggctcgatc
    12001 gaggacacgg cccggcaagc gtagccgaag gccgtgacag ctcatcggtc acgaggtgga
    12061 ccgatgcggt ggcgggtacg acgacgccgg ccgatccccc ctgagggttc tcggccggcg
    12121 tctgcgcaaa cggagcgtcg ggctcggcta gaccgtgcgc cgaccgctca gcagctggaa
    12181 gaccagcacg atggccgcga tgaccagcag cagccagatg aggccaccac cgatgttgcc
    12241 gatcaggcca aggagccaga ggaccagcag gatgacgatg atcgtccaga gcatccggtt
    12301 ctcctctttg ttgtcagatc ccctccgtgg ccccctcgta tcgcatgatg cgtgccagca
    12361 cgctcgccga ccgctcaacc gccgtgcggc cgacgctcct cgatcacctg gtacacgagc
    12421 agcaccgccg cggcgagcag gaacaggttc acccactggc cgagctgcag gatcacgccc
    12481 gccagccaca gcagcagcgc ggccgcggtg agcacccaga tcacgggacc gggttgaaca
    12541 gcacggtgat ggcgagcacc gccaggatga ccagcaggag gaccgatgcc acgatcaccg
    12601 tggtggcgag gtccacggcg cccggcctca cgccgcgagg acctcgagca gctggtcggt
    12661 gtgctcggtc ggcttgacct gcggcagcac gtgctcgatg cggccgtccg ccccgaccac
    12721 gaaggtcgtc cgctcgttcc cgaagtagtt gcggcccatg aagttcttct cgatccagac
    12781 gccgtaggca tcggccaggc ggtgctcctc gtcgaccagc agcgggaagg gcaggtcgta
    12841 cttggcgcgg aacttcccat ggctcttcgc gttgtccggc gagaccccgt agatggccac
    12901 cccggtcgcc gagacatcgg tccagcggtc gcgcaggccg cacgcctgcg cggtgcagcc
    12961 ggcagtgtcg tccttgggat agaagtagag gacgtagcgc gaaccggcca ggctctcgct
    13021 ggacaccgtg ctgccgtcgt ccgcggtgag ggagaagtcc ggcgcgcggt cgccggcggt
    13081 caatcggctc atgggaggag agtctagcct ccggcgtcgc cgatctcctc gatggcgacg
    13141 agcaccggcg gcagctcccg gggctccatc cggctcacca gcgcatcggc gaaggcgccg
    13201 atggcgccgt catcgacctc cgccagatcg tggccggcgc ccgggatccg ctgcaccagc
    13261 gggtcgttcc cgccagcgcg cagcgcatcg gccagcaggt cggtctcgtc cggtgccgcc
    13321 cacgcgtcct cgctcccgtg ggccaggacc acgttgcggc gcagcatggt gccgagggcc
    13381 agcggcggcg tgtggaacgc ctgctcgtac ggcgccaaca tcagctgcac cggcccgtcc
    13441 acgtcgatgg tcatgctcgg ctcgcggcgc tgcgctcgct cgatcagctc ctccgatcgc
    13501 cgatcgaggg cgacgacgat cgggtgccgg aggtcggtcc ccgtgcggga gcgcgtcgcc
    13561 acgccgcggc gcaggacgtc gcggaacgaa cgcgccgagg cgccgaccag gcccacggcg
    13621 ccgaccgccg ggtcggcgat ggccacgctg agcgcgatcg cgaccccctc gccgtgcccg
    13681 acgatggcgg tgcgccgcag gtcgaggtcc cgtcgcgagc gcatggcgcc gagcgcatcg
    13741 cgcgcgtcgt cgatgcgggt gaagaggtcc gtggcagccc agtcgccctc cgactcgccg
    13801 cacccgcggg ggtcgtagcg caagctcgcc acgccccgcg ctgccagcgc atccgccagc
    13861 cgggccagca gatgggggtc gtcggtcgcc ggccgcgcga accagccggg gtggccggcc
    13921 cggtcccacg caccgtcacg gtcgcgcgga agccaggagc cgaccagcaa tgcccacggc
    13981 gcgcgaccct cgacgccgtc gggcagggtg agcgtgccgg ccagcatcag gctgccggcg
    14041 cggaagcgga ggtcgtcgga gcggggcatc gggccgatgg tacgctcgcg gcccagtgac
    14101 cccgccgctc cccaccctcg agccgtcgtt cgcgcccgcg cccgagccgg tgcccttcac
    14161 gccggacccg cggacggcgc tgccgcccgc cgcggaggtc gtcgtcgtcg gcgccgggat
    14221 ggtcggggca tcggccgcgt accacctggc ccgcgccggc atgcggccgc tggtgatcga
    14281 ggccaacgcg cccgcctggg gtgcctccgg gcgcaacgcc gggatggccc tggccgggct
    14341 gggcggccac ttcccacggg tgacgcggct ggtgcaggag gccggcggcc ggtcgatcct
    14401 cgactacacc atgcgctcgc tcgacatgct cgaggtttgg gatcacgagc tgggcggcca
    14461 cgggatcgag tgggaccgct tcgggtcgct ggacctggcc gtcaccgagg ccgaggaggc
    14521 gcacatccgc cacatggccg ggatccaggc cgcggagggc ctggaggtcg aggtcatcgg
    14581 cgccgatgcg ctgcgcgagc tggcgcccgg cctcgccgtc gaggaggtgc gcgccgcgaa
    14641 gtggacgccg cgcgacgcga agctcaaccc gttccgcctc tgctacgcgc tgctcgagga
    14701 cgccatggcc cacggcgcga cggtcgccac cggcgtgcgg gtcgacgcgc tccgggcgca
    14761 cgcgggccgc atggcgggcg tcgagacgtc ccacggcccg gtcgacgcgg gtgcggtgct
    14821 ggtggcgacc aacgcctaca cgccggcgct ggtgccgtcc atcgccgcca acctgacgcc
    14881 catccgcgag acggtgtgcg tcaccgagat gctgccgttc tccatcggcc gacccggctt
    14941 cgagaccaac cagtgcaacg agtactggcg ccagatgcgc accggcgagg tcgtcatcgg
    15001 cggcttcgcg gtcgccgacg aggcgatggg catcggctcc tactcgatca ccgtgcgccc
    15061 gcaggtcccg ccgctgctcg ccggcctgct ggcgcgcctg cacccggcct tgaccgatgc
    15121 gcgcatcgtg cgctgctggg caggactgct cgacttcgcg tcgctggaga tgccgatggc
    15181 gggggctctg ccggcgtccg acggcacggc cgtgccgggc gggtacgtcg cggccgggct
    15241 gaccggccac ggccacccgt acgcgcccat cctgggcctc ctcctggccg agctcatcac
    15301 cgagggcgag gcgcgaacgc tgtccctggc gcccttcgat ccgcgccgct acgtcggcga
    15361 gcggcacgaa ccaacgtggc tggagccctt ccggggcgag acgccgatcg agcgctgaat
    15421 tccggcggca gcgtcctagc cgatgagcgg tcgccaggtc ccgagcgcca ggctgacacc
    15481 gatcgcgacg gccgccagcg ccgccgcggc ggcgatccag ccccagtcgc ccgccgtcat
    15541 ccgctggggc cgagcgacgg ttcgacagtc ctgcgccccg aagccgcgcg cctccatcgc
    15601 ggccgccatc cgcgtgccgc ggcggatggc cccgaccagc agggccatga gctgcccggc
    15661 gaagagccgc accgcggcga cggggtttcg acccgagtcc acgccgcgcg cgcgccgtgc
    15721 catcgacagc gtctgccact cctgcgcgag gagcggcagc agcctgacgg cggccagtgc
    15781 cccgatcgcg aagcgcggcg agacgcgcag ctgctggacc agcgcatggg ccagctccgt
    15841 cggctgcgtg gtcgcggtgg ccaggatgcc cgccccggcg atggccagca gccgcagcgc
    15901 gaggccgagg ccgtcgacca gcgtgccggc gccgatgcgc agcggcccga gcgaaacggc
    15961 cgtcggcccc acctgctcgg cggcgaacac cacgttgaag accgcgatcg ataccgcggc
    16021 gagcgccacc agccacgccc gcgaggcgag ggccgacggc gagagacccg acatgccgag
    16081 cgcgatggcc aggcccgcca ggatgacgag cgccgtgacg tggtcgagcg atgcgaacag
    16141 cgcgaccagc agcacgaggg cggcggcgag cttggcaacc ggattcgcgc gcgccagcgg
    16201 cgccgcggga tcggggacca gcggtgtgag cagctgcatc agcccggagc ctcgagcgtg
    16261 atcacccgat cggcgagggc gcggatgaac ggcgggtcgt ggctgacgac gcagatgccc
    16321 cgcccctcgt cccgcagccc ggccagcagg tgcaggagct cggcccacgt ccggcggtcc
    16381 tggccgaagg tcggctcgtc cagcaccagc gcggcgggtg cggtcgccag tgccgtggcc
    16441 accgagaggc ggcgcttctc cccgcccgac agggtgtacg gattggcgcg ggccagcgcg
    16501 gccaggtgga gtcgctccag cagctcgtcg gcacgcctgc cggccgccgc ttcgctcacc
    16561 ccgatccgcc cgggcccgag cagcagctcc tcgcgcacgg agccagtgag gaactggtgc
    16621 tccgggtcct ggaagaccgt ccccacgtgc cgggcgagct cgcgggccgg ccagtcggcc
    16681 agcggttcca tgccgcggcc gggagccagc gcctcggtgg cgcgaaccga cccggcgctc
    16741 ggccgcagca ggccggccag cagcatggcc aacgtggact tgccgctgcc attcggcccg
    16801 accacgccaa gcacctcacc ggcgcgcagc tgcacgtccg tcggtcgcag cgcgtcacgg
    16861 tcggcgccgg ggtagcggaa catcgcgcgc tccgcgatga ccagcggctc gggcccgggc
    16921 gcttcgcgtc gaacgggcgg cgccggcgga tggtccggca cccagacgcc cgcctccgcc
    16981 agccgggaac cctccgcgcg gaacacctcg acgggcggcc cgtcggcaac cacgcctccg
    17041 cccgcctgga gcaccacgac ccggtcgacc agcggcagca cgtcgtccag ccggtgctcg
    17101 accaggagca gcgtcacccc ggttcgctcg aggatgcggg cgacggcgcg acggacctcc
    17161 gccgcaccgt cggggtccag gttcgccgtc ggctcgtcga gcatcatgag gccgggacgc
    17221 agcgcgagcg tcccggccag cgccagccgc tgctgctcgc cgccggagag ggcgtgcgtc
    17281 gggcggtcgc ggccgtacgg gaagtcgacg gccgcgagcg ctgcatcgac gcgcggccag
    17341 atctcggggg tcgggacgca tcggttctcg aggccgaagg ccacgtcgtc gccggcgcgg
    17401 cccatgacga tctgcgcctc agggtcctgg aagagcagcc ccgtgcggtc tctcgcgtcg
    17461 cccgggtcac ggccgtcgac gagaagggcg ccctcggtct cggcgccccc ggcgggatcg
    17521 atcaggccgg cgagcgcggc cagcagcgtc gacttcccgg cgcccgatgg ccccagcagc
    17581 agcacacgct cgcccggctc gacgacgagg tcgagcccgc gcaccgccca cgcccggcgc
    17641 cccgggtagc gcagccccca gccacgcgcc tcgaacccgg ccgcccccgt gggcccatcg
    17701 gtcacggacg cgagagcggg ggcagcacgc tcagacctcg cgctgctcgc ggccgacggc
    17761 gaactcggcg agcacacccg tggccaccag ggctcgaagc aggtaccacg acccgattcc
    17821 ggcgatcagg atgccgctga tgaccgtggc gaccgcgaag atgacccaga agtcgacgcc
    17881 cagatcgggg tagtagacgg cgatgtcgtg gatggcggca ccgatgccgg ccagcgcacc
    17941 ggccagcatc gcgaagggca ggttccagtt gcggtacatc cccagggcga aggcgagctc
    18001 cgccgcccca ccctggatcg cacccgagat caggacgtcg actccccagg gtgagccgag
    18061 caggaccgat acggccgccg acacgaagcc gccgaagaag gcggcccccg gacggcggac
    18121 gatcagcccg acgagcacgc ccggcaccag ccacacgccg tagagaatgg cctgggccgg
    18181 cttcagggcc gcgaagatcg ggtccgtcgc ggtgtagatg gcgttccagg cgaggaagac
    18241 gacgccgaag gcgacggcga cgacggcgcc gacgacgatg tcgacgatgc gccagccgcc
    18301 ggccgagctc gtcgtggtgg tcatggattg gctccttccg tgctgctctc gacgaccagg
    18361 agcgtcccct cccgcagccg gaccgatgcg ggcgccgcca cgacctcgtt cgagaggccg
    18421 cgggagcggc ctgcccgcag ggtcgcatcg gccagcggcc agcgcaggcc gtcggtcgtg
    18481 acgccaatgg cgtcagcggc gagtgggagg aggctgacga tcgcgccggg cgcgccctcg
    18541 agggccagca ccgagtcccc gtgcagcacc cgcacggtcg tcgtcccgcg gaccagccgc
    18601 aggtcgacct tcgcgaacgc cggatcggcg agcagcagca ggttggcgag ctcgtggtcg
    18661 agccgctggc cacccagggc gcccaggagg atcagctccg tcgcgccgga ggcgatcgcg
    18721 gcctcgaggg ccagctccgt gtccgaggcg tccttgtcgg ccgggtggcg ttcgatcccg
    18781 acgccccggg cccgaagacg ctcgacgagc tccgcatcgg tcgagtcgag gtcgccgacc
    18841 aggcggtcgg ggacgcgcgc catccgggcc agcgacgacg cgccgccgtc ggccgcgatg
    18901 accagctcgg ccgaatccag ccaggcgtcg tccgccggct cgatcgcgcc cgaggccacc
    18961 accaccactc gcatcggctc ctcatcgcgt gctgtgctgc cgaaacgacg tgcggcgcgc
    19021 tcgaacccag ctcgggattc ggggcgcgcc gcggacgagt gctgcgtcga cttccttcgc
    19081 cggtatgacc cggatcaggt tcgagggtct gcggccacgt gagccgcact ctcagcgctg
    19141 tcgcgctccc ctgtcgtttc gtggattgtc ggatcagcct agcacgggcc gtatgatcgg
    19201 tcgatgcagc cgaccgacgg atccgcgcac cccaacgtcg agcgcgtcgt ggccgcggcg
    19261 cggaacgccg gcgtcgacat cgccgtccag cgcttccccg aagggacgcg gacggcacag
    19321 gacgcggcgg cggccgtggg ctgcgaggtc gggcagatcg tgaagtcgct ggtcttcgtc
    19381 gccgacggtc ggccggtggt cgccctcgtg tcggggacca accggttgga caccacgcgg
    19441 ctggcgtcga cgctgggcgc cacggccgtg cgccgcgcgg acggtgacga ggcgcgcgcc
    19501 gccacgggct tcgccatcgg cggggtcccg ccgttcggcc acagccagcc gctggacgtg
    19561 gtgatggatc acgacctggc gacgtgggag cgggtctggg cggccgcggg gctgcccgac
    19621 gccgtgttcg acatcagccc gggggagctg gagcgggcgt ccggagcccg cctggcggac
    19681 atcgcggagc ggcccggggg ctgacgcccg ctatcctccg cccgatgccc ctcggaaccg
    19741 tcttcgccgt cctgctcgcc gccggcctcc tgctcgtcgc cggctgtgcc gcgctcatcc
    19801 ggatcggtgg ggcgcaggcc tccctggcac gacgcctggc cggccccgcc gaggtcaagg
    19861 tcggccgtct gctcgacgcg gacgagctgc cgcagcgtcc ggtccgggtg gtcggccgca
    19921 tccgctgccg cgatccgctg aacgccggcg atggggagcg cctggtcgcg taccaccgcg
    19981 acgtcgaggt gcgcatcggc ggccgctggc gctcgatcga acggctgcgc gagacgcgct
    20041 cgttcgacct gtgggaccac gacggttcgg tgccggtcga tcccgcccgc gcggcggagc
    20101 cgctgatcac gattccgaag gtgtggcggg gcagcccggc ggagctcgag gagccccacg
    20161 cgtcggccgt cgcgcggctg gccgagcgcc acggtccggc gacggaggca cgcgcggtca
    20221 cgcgcaccgt caacgtcacg gaccggctgc tggtgatcgc gcgaccgatg cgtgacgacg
    20281 ccgggcgcct cgctctggag ccaccggagg gcggcttcct ggtgtcgacg ctcgacctgc
    20341 cggacgcgat gcgcctcctc ggcggccgcc acaaccggct cagcgccctc gccgtcgccg
    20401 gcatgggggt cgggatcgcg gtcgccgcga tcggcctggt cgggctcgtc gccgcctcgc
    20461 tgatcggggc ctgacgccgc ggcggggcgg ccgcatcggt acggggtcct tcgggaccat
    20521 cgtggcatcg tcgcggtgag ggacggtttc tagcatcggc tgcacgagac ctaccagaag
    20581 ctccatccgg accagtggag attccatgcc gagcgaccag accgatcccg gcgactccct
    20641 cctcagcgtg gcccaggcgg ccgcggtgct cggcgtgcac cccaacacga tccggacgtg
    20701 gacggatgcc ggacggctga ccgcctaccg catcaacgcc cgcggcgacc gccgcgtccg
    20761 ccgtcgcgac gtcgagcggc tgctcgtgga ggatgggatc gccgccatgg agccgacggc
    20821 tccggcgatc gcggtcgacg gcgccgtcca cgaccgcgtc accgccacca tcgcgcgcct
    20881 ggcgcagaag ctcgccgcct cgcagtcgcc ggcgggcctc gcccgggcca cggtcgaggc
    20941 ggtccgcgcc gatctcgggg ctcgggccgc ggccgtgtac ctcgccgccg acgacggctt
    21001 caccctggcg gcccacgcgg ggttcgacgc tcccccggcg ccgcaccgat ccctcgacgg
    21061 cgcaccgcct ccgcacgagc cgaccgctgg cctgctggag gcaccgatca cggggagccg
    21121 aagcgtcctg ggcctcgtca tcgccgatgc cggtggctcc cgcgacgtcg agctcctgct
    21181 gcgggccgtc gcggccaccg ccggcgcgac gctccaacac ctgcgcctcc tgggtcgggc
    21241 gcgccggcag ctgcagcgct cgcgcgcgct gcgatccgtg accaaggacc tcaccggcac
    21301 cctggatctc aactccgtcc tcggcgacgc cgtcgagctc acccgctcgc tgttcgaggc
    21361 cgagaaggcc ggcctgtggc tggtgaacga cgcgaagcac ccgttccggc tggcggcgca
    21421 gcacgggctc tcgccggcgt tcctgggcca cgtcgcggac ctgcggctga gcgacgagac
    21481 gctcggcgtc cgggccgcgc gcgagggccg ctccttctgg ctcagcaacg tcgataccga
    21541 cgcgggcgcg gggagcatgc gcgagatcta cgccgcggag ggcatcgtca ctgcctgcct
    21601 ggtgccactg gtcgggcgcg acgagacgat cggcgtgatc gcgctgtacc acacgagctc
    21661 ccggatctgg agcgacgact cgctcgagct ggtccaggcc ttcgccgacc aggccgccgt
    21721 cgccatccag aacgcccgcc tctaccgctc cgtggctgac caggccgccc gcatgcgctc
    21781 gatccaggac ctctcggcgc ggctcaaccg gctcaccgac gtccgcgcca tcgcgcaggc
    21841 catcgtcgcc gaggcgaaga cgctggccga gtaccacgac atccgggtct accgggtcga
    21901 ctgggagacg ggctggtgcg agccgatcgc cttcacccgc gagatgctgg tcggtgatcc
    21961 ggcggacgcc gagtcgctgc tgcgcgtgga gatcggtcag ggcttcaccg gctgggtcgc
    22021 cgagaacgcg gagccgctcc tcatcaatga cgccgtcgac gacccgcgcg ggcagaccat
    22081 cgaggggacc gacgacgtcc ccgagtccat gctggtcgtg cccatgatct acgaggggcg
    22141 caccaccggc gtcatcgtcc tcagccagct cggctacgag cgcttcagcg ccgatgacct
    22201 ccagaccatg acgatcttcg ccggctacgc cgcccaggcg atggcgaacg catcggccta
    22261 cgagcagctc cgcgaccagt cggcggagct cgcgcgccgg gcggactccc agcgccgact
    22321 gctggagatc aacgagcggc tgctgtcgac cctcgaccag gccggcgtgc tggagaccat
    22381 cgccgatggc ctgcgcgacg tcgtgcgcta cgacaacctg tccatctacc gcaccgaccc
    22441 gcagcagcag gcgatgctgc cggtgctcac ccgcgagcgc cacgccgagc aggtcagccg
    22501 ctacatcatt ccgttcggcc ggggcctcat gggctgggcc gtccagcacg cgcagccgat
    22561 cctcgccaac gacgccctca gcgacccgcg ggcgctccag atccccggga cgccggagga
    22621 cccggaggcg gtcgtggtcg tgccgctcat cgccgacggc gaggtgctcg gcgccctcaa
    22681 cgtctcgcgc gtcggcggtc cggaggtgca cttcagcgag agcgacttcg agctggtgca
    22741 gctgttcgcc gcccaggcct ccatcgccct ccgcaatgcc gatacgcacc acgcggtcag
    22801 ccgcctggcc gagaccgatg cgctcaccgg cctcgggaac cacggcgcct tccagcgcga
    22861 tctcggccgc ctgatcgagg cacgcggcac cgccgcgaca cggggcggag gcctgaccct
    22921 gctgatgatg gacctggaca gcttcaaggc cttcaacgac gtgcacggcc accctgcggg
    22981 cgacgcgctg ctgcaccgcg tggcgatggc gatctacggc gcggctcgca gcgaggatcg
    23041 cgtctaccgc tacggtggcg acgagttcgc gctcatcctg cccgcgacca cggccgcgca
    23101 cggcgcgcgc atcggcgagc gcatccggac ggccgtggca gcgctgacgg cccacgaccc
    23161 gacgccggtc accatcacgg tcggagtggc gacgctgacg cccgacgtgg cggaccgagc
    23221 cgcgctcgtc gcggcggccg acacggccct ctactacggg aagcgggcgg gggaggaccg
    23281 gatcgtgcgc ttcgatcgga tgccacccga cgtcggcgac ctgcgaggaa cgctcgacga
    23341 cctggccgcg gcggcgctcc gagacggcga cgatgcccgc gtggtcgagc acctggtcga
    23401 gcgcgcctcg cagctgggtg cggccgcaca ccacgatgcg gccgcgtcgg tacgcgacgc
    23461 gctgctgacc gtcgcccgcg ccgtcgacgg ccgcggccgc accaaccgcg gccacgccga
    23521 tcgggtcggg cggctggccg gtgccctcgc catgacgctc cacctcgacg aggacgtgcg
    23581 ccgcaccgtg gagctggccg gacggctgca cgcgctcgac gaccgcggtg tcgccgagat
    23641 cagcagcgtc ccgtcgctgc acgacgtctc ggcgctcatc gtcggcgccc ggcgcctcgc
    23701 cgacgaggga gcgcggcgcg cccgtcggac gtcccggatg cgcggccgcc tgggcgcgca
    23761 catcgtggcc gtcgccaacg gctacgacga gctcgtctcc ggcgtcggtg gccctcgcgt
    23821 cggccgtcgc gaggcgctgg cgcggctggc atcccacccg agcagctacc gcgacgaggt
    23881 gctctcggcc ctggccggca tcgtgggcga gcgccgtgac tcgggacgtc gccgccgagt
    23941 ctccgattcc gccggtgagg ttccgggagc cgcctgaccg accagcgcac tgataccatg
    24001 gcgcgccgat gacccacccg cgcctcgccc tgccccgcac gtcgggagcc gtgggcgatg
    24061 gtcgcgcgag gaccaggagg ctcggcgccg gcctcctgat cctcggaacc ctgctcgccg
    24121 cctgctccac cgccgaggtc cagcccacgc cgaccggcac ggcggcaccc acctccgcca
    24181 ccgccagcgc ctccccaaca ccgacggcca ccccctcccc gaccccggag ccgacgccgc
    24241 gctacacgaa cgagccggac gaggacctcg cggccctcat cccgaccgag gcggccggtg
    24301 tgccggtcgt ggtcgccccg ttcgacgagt tcgcgttgac gccgggagac gtggccatcg
    24361 cctacggcga gctgggcaac cgcttcgcgt cgctggtggt cgcctacgtc gagcagccgc
    24421 gcacgacgct gtacgccatg cgcctggacg gtgagccggc cgtcacggag gacctcgagc
    24481 tgttcctggc gacggccggc cgctacgtcg gcatcgcggg cctcgacccg gatccgtgga
    24541 cgctggacga cagcgccggg taccgggtct gggtccgccc cgaggacaac gccacggcgg
    24601 ccggcaccat ggtctacacg tgggccgcgg aggactacgt cttcctgctc atcggcgtcg
    24661 acgacaccgt caaccgcgcg atcatcgagc agctgccgac ggagcccgcg ccgacccccc
    24721 gtccgaccgc ctcaccgtcc gcatcggcgg ggagcagcgc gcccgccagc gccagtccct
    24781 cgggcggctg agccgatgcg gatccgcgag ctggccgagc gcgtccgcca cagtctctgg
    24841 ttcatcccgg ccctcgcggt cgtcatcgcc gtcgtcgtcg cgctcggcat gctggcgctg
    24901 tcggaccggc ttgacgccag tggtgcacac ctgcccctga tcttcggtgc cgggcccgac
    24961 ggcgcgcgtg cgatgctggg agcgatcggt gggtcggtca tcacggtcgc cggcaccatc
    25021 ttctcgatca cgatcgtggc gctgcagctg acgagctcgc agttctcacc ccgcgtcctg
    25081 cgcaacttcc tgcgcgaccg accgaaccag ctggtgctcg gggtcttcat cgggaccttc
    25141 acgtactgcc tgctcgtgct gcggtcgatc cgcgccgagg cgaccgaccg cgccgccttc
    25201 gtgccgaacg tggcggtcac cggggccctg ttgctggcct tcctctcgat cgggatgctc
    25261 atctacttca tccaccacgt cagcgtgcgg atccaggtga cctcgattct cgccagcgtg
    25321 gcgagcgaca ccctgcacac catcgagacc gtcgctgact ggctcgagga tcggcccgag
    25381 cgcgcgtggc gctcggccgg attgctggac cgcgtcccgc ccgccccacc gaccggcgcc
    25441 atcccggccg accgggcgcg ggtccggatc gcggcgcatc acagcggcta cctgcagttc
    25501 ctggacatcg ggcggctggt tcgcgccgcc gccgaggccg agggccggct cgaggtcctg
    25561 gtggcgccgg gcggctgggt gcagcgcgac gcgccggtcg ccaccttcgc gggacccgac
    25621 gacgccgaca ccgaacgcct gggcgacgcc gtcaacggcg ccctctcgct ggccgccgag
    25681 cgctcgacgc agcaggacgt ggccttcggc atccagcaga tcgtcgacat cggcgcgaag
    25741 gcgctctcgc ccggcgtcaa cgacccgacc accgcgatca acgcgctcga tcgtctgctc
    25801 cagatcctgg tggccgcggg tgtggcacag gacccgcccc aggcgttcgc ggacgcggat
    25861 ggcgtgaccc gcctggacgt gccgatgccc ggccttcacg agctggtgga gctggcgctc
    25921 gaccagctgc gccactacgg cgcctccacg ccggcggtcg ccctgcacat ggcgcgggtg
    25981 ctcagccagc tgcgtgacgc cgtcggggag cgtgccgccc cggccatcag gcgtcaggcc
    26041 gacctcctgg tcgaggccgc cgcccggatc ccgctcgcga gcgagcgggc ggcgatcatg
    26101 gccgccgtcg cgcccctgcg atccgcctga cgaccgtcga tcgcggctga tcgcgccgcg
    26161 cggcacgcgg ggtgcgtacg atgcccggca tgttcgccaa ggtcgcgtac gggctcgcca
    26221 tcggcggcac gatcctcacc ctcgcgctcc gcgtcttcgg ggcggacgag acgctgacct
    26281 tcggcgtcag cgccatcacc atcctggggc tggcctacgt gctcgggcac gccaccgagc
    26341 agctggggat ggcgaccggc ccgcgcatcg gcggcatcat gaacgcgacc gtcggaaaca
    26401 tcggcgagat catcatcgcc gccttcctga tcctggacga caagatcgag atcgtccacg
    26461 cctcgatcac cgggtcgatc gtcgggaacc tgctgctcgt gctgggcgcg tcgctgctgg
    26521 tcggcggcct gaagaacggg gtgcaggagt acgacgagca gctgaccggc atgaacgccg
    26581 cgtcgctgat cctggcgacc atcggcctca tcgtcccggc gaccttcgcg ttcgtgatcg
    26641 gcggcggcga ggggaccgcg cccggcgacg ccgagttctt ccagatcgag gccctcaccg
    26701 tcggcgtggc ggtcctgctg atccttacct acgccgccca gacctggttc ttcctgcggg
    26761 cgccggaagc gccggcggcg gcgcggcacg aagaggagga ggtccccgag tggagctgga
    26821 aggtcgccct cggcgtgctc ggtgcgtccg cggcggcgct gacgttcgcc tccgaggtgc
    26881 tggtccagac cctcgagccg gccgtcgcgt cgctgggcat cagcgaattc ttcatcggca
    26941 tcattctgat cccgctgatc ggcaacctgg ccgagcacgt ggtcggcgtg acgctggcct
    27001 ataagaacaa gatggacttc agcctgatca cctcgatcgg gtcggcgacg cagatcgcgc
    27061 tgttcgccgt cccggtgctg atcttcttcg ggctcatcgt cggccatccc gtgacgctgg
    27121 tgttcacccc gctcgaggtg gtcgcggtcg cggtcggcgt gttcatcgcc ggcttcatcg
    27181 cgctcgacgg gcgctcgaac tgggtcgagg ggctgcagct gatcagcgtg tactcgatcc
    27241 tggccatcgc cttcttcttc ctcacgtagc tcggcacccg acgggccgcc ttcccgatcc
    27301 gcggcctaga atcgggccgt gttgaccctt ccgggacccg tcgtcgacgt cgactggctg
    27361 gccgcccacc gcgccgatcc gtcgttgcgg gtggcggacg tccggtggtc gctggccggt
    27421 ccggcggggc gcgagctcta tgcgcgcggc cacgttcccg gcgcggtgtt cctggatgcc
    27481 gagaccgagc tgtcgtcccc cggcgagggc cccggccgcc atccggtgcc cgccgccgac
    27541 aggctggcgc gcgtcctcgg ccagcggggc atcggcgacg agcacgtcgt ggtcgcctac
    27601 gacgatgccg gcggcagcat cgcggcccgc ctgtggtggc tcttccggca ctacggccat
    27661 gacgggcagg ccgccgtcct ggacgggggc atcggtgcct ggacctcgac cggccttccg
    27721 ctcgagaccg atccgccggc ctacccgccg gcgacctgga ccgtccggca cgtcgccgat
    27781 gacctggtcg gaaccgatga ggtggtcggg atgctggacg gcgagacgct gctcctcgac
    27841 gcgcgggcgc gggagcgata ccgcggcgag accgagccga tcgatccgcg cgccgggcac
    27901 atccccggcg cggtcagcgc gccgcacgcg gacaacctgg gcgccgacgg acgcttcctg
    27961 ccggccgatg cgctgcgccg gcgattccgc gcgctgggcg tggcggacga ccgaccggtg
    28021 gtcgcgtact gtggctcgtc gctgaccgcg acccatgacc tgctggcgct cgagctcgcg
    28081 ggcctgcccg gcggtcgctt gtacgaggga tcgtggagcc actggtcgtc cgatccgagt
    28141 cgtcccgccg tgacgggagc agcgccatga ccggttcgca cgacgagtgg gatggctggg
    28201 acgagggggc ggccgaggac cgcatcggcc gcaagcgcga ccgcctcgcg cggctgctga
    28261 gcgtcgcctc catcctctac tcgcgtggct ccggcgagaa cggcgtcgcg gtcagcgaga
    28321 tcgcgaagct gaccgggatg accacgcgca ccgtgtaccg cgacatcaac gcgctcgacg
    28381 aggagctggg ggtcccggtc ttccaggccg ggcgcggccg ctacggcatc gatcgcaagt
    28441 tcttcctgcc gccgctgcgc ctgagcgtcc cggaggccat cgtcctgttc ctggcgtccc
    28501 gcctgatcgc gcgctggagc gaccagtacg acgcctcggt cgtcagcgcg ttcaccaagc
    28561 tggccgacac cttgccgcag ccgatcgcgc ggcacgtcgc cgccagcatg ctcaccatcg
    28621 gcgccgccga cccgaacgag ccgttcagcc gctccttctc ggccgtcgcg cgcggctggg
    28681 ccgagggccg ggtcgtcgag atcgactacg acccggggac ggcgccggcc aagcgcacgc
    28741 gggtgcaccc ctacttcctg gaaccggatg ccgcgctgcg cagcgtctac ctcatcgcgt
    28801 acgacgagcc ggccggcggg atgcgcacct acaaggtcga gcgcatccgc tccgccaccc
    28861 tcacgcagga ccgatacgag atccccgagg acttcgaccc cgacgcctgg ctcgccaact
    28921 cgtggggcat ctggtccccc gattcgacgc cgccggtcca cgtccggctt cggttcgagt
    28981 ccgaggtagc gcaccgcgtc cgcgaggcgg tgtggcatcg gtcgcaggag ctgaccgagc
    29041 tgccggaggg tcgcgtggag ctggcggtga cggtcaacgg catcgtcgag atccggccgt
    29101 ggatcctgtc gtggggcgag tcggtggagg tgctcgagcc gccggagctg cgcgaggcca
    29161 tcgccgcctc cgtccgtggc gcggcggcgc gctatgccga ctgaggtcga ggcgcgcttt
    29221 gcggccgacg gcccgctgcc cctcgaggcg ctcacgcgcg ccgcgcgcct tggcccggcg
    29281 gtgctcgggc ggccacgcac ggccgacgag accgaccgct acctggacac cgacgacgga
    29341 gccctggcga cggcccgctg ggcctgccgc ctgcgcaccc gcgacggcct gacccgcatc
    29401 tcactcaagg gcccgccgga ggctggcgac cgagatgact ggcaccaccg ccgtcccgag
    29461 ctggaggggc cggcgaccga ggagctggac ccctccgcct ggcctcccag ctctgcgcgc
    29521 gacctgctcg accggttgcg tggcggcgct ccgctgcgcg agcggctgcg cctggtccag
    29581 cgccgcaccg agcgctcggt cacgctcgac ggcatcccgt tcggcaccct gacctgcgat
    29641 caggtgcgcg tcgagcggga gggctcagcc gcgggcgacc tccacgtcgt ggagctcgag
    29701 ctccacgacg aaggcggggc agacgcccgc gccgcgctgg agaccctggc cggcgccctt
    29761 gacttcatgc ccggcctccg gcctgagccg cggacgaagt tggagcgggc gctggacctc
    29821 ctcggcgagt cgcggccgtg acggcggtcg cgtcctgggt ccggcgccgg accgaggccg
    29881 aatgggtggc gctggccgcc gcggctgcga ccttcggcta cgtcgggtgg gacggcgcgc
    29941 tgtgggacgc gcgcttccag ctgctgctcc acctgctggc catcggcgcg atcgcggctc
    30001 tcggcgtgct cgcctggaag ggcggcagcc tgccgcgcac ccgcatcgac gcgccgctgc
    30061 tcgcgctggt cgccgcgttc gcgctggcga cgacgtcggc gctcaacgtc gggatgagcc
    30121 tgcgcgccat gggggcgatc gtggcgacgg ccctgatgct cccggtggcg ctcgtcgcgc
    30181 tccgacaccg ggcgagctgg gtcggcctga tcgtggccgt cccggtcgtc gtgctctcgg
    30241 tccccgccct cgtcgtgctg ctgggccgtc gcctggagtg ggtgctggcc ggcgcgccgg
    30301 gcctgccacc catccggctg ccgggcgagg gcacgccgtt cggctcggtg gccgtgccgc
    30361 cgttcgtcat ctggccggcg tgggcgatcg cgggcgccat cgaggacgac cgcctgcggt
    30421 ggtgggtccg cgcggccctg gtggccgtcg gcgtcccgct caccatcctc tcgggctcgc
    30481 gctcggcgtg gctggcgatc ggcgtcaccc tgctggtcgc cggcctcccg tgggcgtggc
    30541 agcgccgcca tcggctcgac ctcaaacgcg gctggactcc gcggcggatc gccgtcgccg
    30601 ccggtggcct cgtcgtggtc gccggactcg cggcggtgct cctgccccgc gtaacggcgg
    30661 tcacctcgct gctctaccgc gccagcctgt ggcgcgacac gctgcgcgcc tggtcgaccg
    30721 atccgctctt cggcatcggc ccgggcttca tgccgtacgc gcggcaggcc gccgcgcccg
    30781 acttcagctt cccggtccgc cagccgcact cccacaacct gccgctcggc gtgctgggcg
    30841 acgcggggct ggtcggcctc gcggcggcga tcgtggtcgt cgtggcgctg gccctggtgg
    30901 ccggcccgtg gcgcagcagg acgccggtcg gacgtgccgc ctcgctcacg ctgctcggcc
    30961 tcggcatcgg gggcctgttc gaggacctca ccttcctgcc gaacttcaac ctgctggcgg
    31021 tcctgctggt cgccctcgcc ctgaccgatg ccggcgccgt cgactgggtg cggcttccgc
    31081 gcccgccgct gcgtcgggtg gcgctggggc tcgggactct ggcggtggcg gcgccgctcg
    31141 tcacggcgat ggtgatcgcg gacgcgggcg cggtcgccta tcgggcgggg gtggacacgg
    31201 ccgcatccgc gcgctggacc gacgcgacac gctggttcgg acgggccacc gagctcgacc
    31261 cgtggcatcc cgccaacccc aaggcgctgg ccgtcgccgc ggacggggcc ggggagacgg
    31321 cgctcgctcg gcgtgccgcg gaggacgcga cggagcgcaa ccccggcgac gcgccctcgt
    31381 gggtgaacct cgccctcctc tgccgcgagc tggccgacga gacgtgccag agccgcgcga
    31441 cggagcaggc cgttgcgacc gcgggcttcc tgcgccccga gctggtcaac gcggcgttca
    31501 gctacgagtc actcgggttc accgacgagg ccgatgacgc ctaccgccgc tcgctgctga
    31561 gccagctgct caccgccttc gcgaccgact ggccccgccc gatcggcatc ggtgacgcga
    31621 ccatcgaaga ccaggccggc gcgcagttcg agctgaaccg cctcgtcgcg tggtgggcga
    31681 tggacgaacc gatccgcccc gagctcgtca ccgaccccgc cacgcgcgcg ctggcgcacg
    31741 ccatgcgcgg ggagcgtgac caggccgagg agtggctgag cgtggccatc gacgaccatc
    31801 ctgaccagat ccccacctgg gacgtggccc tcgtcctgcg ccggcactgg ggcgagccga
    31861 tcgacgacgt cctgcgcatc gaggaggccg tccgcggccg gccgctcacg gagcgcgacg
    31921 ctccgccccc ggacccgaac gcgtcgcccg ggctgacctt cgacatcggc tcgttccgcg
    31981 cctacccgcg ggacgggttg atcgggggtg ccatccggct cgtcacccgg ccgccgtacc
    32041 cgtggatcct cgaggtgctg ctgccgccgt cgggctgacg ccgtgactcg gcgcagcgac
    32101 gcgtcgctcg acgccctcag cccagctccg ggaacggcag ccgctggatc agcgacagcc
    32161 gcggccgctg cgggaccgcg gccggcgtga tcggcagtcg ctgccacgcg tcgcccgccg
    32221 gacccgcgcg gtaacgggcg tccagccggc gggccgagat cgagacgagg ccgaagcgtc
    32281 ccagcgcctc ggccaggagc gtcccctcgc cgcgcaccgc gtgcgagacg acgcagcggt
    32341 cgccgtcgag gagcaccgcc tcgaggtgct gacgccgcgc cgagaaccgg cggcgctgca
    32401 acgaccgccc atcggtccag agcaggtccg tggcgacgta ggccggccgg ccggcgcgcc
    32461 ggtctccggt gaggcgccgg tgcagcaggc tcgtgtccag ccagccgccg tcgtccaggg
    32521 tcagcagcca gccgtcgagc accacgccgt cctcgatgag ccgctccggc agctcatcgc
    32581 ccagctcggc gaaggcgtcg agcgcatcgg ccaggccctc cgcgcggagg cgcatcaggg
    32641 ttcggttctc cacccatgcc tgggcgcgca cgccgggcca ccacggctcg aagaggtagc
    32701 cggggtcgtc gaagggcgag gcgccctcgg tcggctgcat cggatcgatc cgagacggca
    32761 gcgccgagcg cggctcgggc gggatcggga ggtcgagctg ctctccgggc acggcccgtg
    32821 gcgaggtcag gccgtcttgc gacggcgctg acgcggtgcc ttggcggtcg cctccgccgc
    32881 gggctcctcg acggcggccc ggcgctcgga gcgcgcggcc gcgacggacg cctcgagcgc
    32941 ggccatgagg tcggtgatgt tcgtcggctc ggtccgctgg gccggctcag gcttctcgcc
    33001 cgcgaccttg cgctcgatca ccgccatgag ggcctggcgg tagtcgtcgt ggtactcatc
    33061 ggcgctgaag tcgcccgtca tgctcgccac cagctgctcg gccatcttct tctcggccgg
    33121 cttgatctcg atctggtcct cggggaggtt gagctcgtcg atcgcccgga cctcatcggg
    33181 ccagtggagc gtggtcagca gcatggtcga gctgaagggg ttgagcgcgg cgagctgctc
    33241 gcgatccttc aggacgatct tgcagatggc gctcttgttc tgctcggcca gcaccgactt
    33301 gagcaggtag aaggccttcg cgccgatctg ctcgggctcg aggtagtagg cctgcttcac
    33361 gaactgcgtc ccggcgttgt cccgcgacgc gtcgacgaac atctcgatct cgatggcccg
    33421 catcgtcttc agggggaccg agtcgatgtc ctcctcggtg acgacgacgt acttgccctt
    33481 gctccactcg tagccgcgaa cggtctctga gcgcggcacc acctcgtcgt ggtacgggca
    33541 gtagaccttc atctggatcc ggttcaggca gctggcatgg agcaggttga agccgatgcc
    33601 accctgctcg gtcgccaggt acagcttgac cgggatcgtc accagcccga actggatcgc
    33661 cccccgccac atcgatcgcg ccatgaagtc ggtctctccg tgcgccgcgt tccgcgccgg
    33721 ccgtgccctg ccagcgttcc cgatcgcggg cggtgatggt acaccggcct cgcaagcgac
    33781 ccgcacctgc catccgtact gcacgagtac cagccacccc ggcccatacg agccatggac
    33841 cgatgcgcgc gcgcccgcga cactcgcagc gatgaacgac gacaccgcgc ttcgcgccta
    33901 cgtcctcgag gacgaccccg acatcgccgc cctcgaggcg gacctcctgc gtgacatggg
    33961 gttcgccgtg gtcacctgca cgcggatcgt cgagctcggc gacctgatgg agctcgcgct
    34021 gccgcacctg ctcgtcgtcg acgtgatgct gcccgacggc gatggcggcg acatcacgga
    34081 gctgcttcga tccgcgtggc ccggcatccc ggtcgtcatc gtctcggccg ccagccgcga
    34141 gcggctggcc gagctggcgc cgatcggccc ggtcgtcgcc aagccgttcg acctcgagtc
    34201 cttccggaac gccgtcggcg aggcgatcga gggctcgcgg gagctgcgca gcgtctcgtg
    34261 acgcgggggc cggctactcg acccgggtcc cggacgggcc ggtgcgggcc accagcgccg
    34321 cgtgctcgct gccgtccgcg gccgtgagcc ggtaacgatg ctcgatctcc accgacgccg
    34381 acagcatcgc gttgaccggg cagtagcggg tggccgacag ctcgatcgag cggcgcagcg
    34441 cctccggctc gacctcaccg gtgacctggt gctcgatcgt gatggccgtg aagaccctgg
    34501 ggtgttcgcc ggccgattcg ccgcgcacgt cgatctcgta ggtcgcggcc gactggcgct
    34561 tcttgcgcag gatgctcgcc acgtcggcgg cggcgcaccc ggcgagcgcg gccaggaccg
    34621 tctccttggg tccggcggcc gagtcgcggc cctccggcgg atcggcgtcc agggtgatcg
    34681 gctgcccctt gggcgttcgc gcctcggtcc ggcagccggt cggatcgacg atcgcgtgga
    34741 tggattcgct catgcggcca gcctaccgtg ggggcccggc cgatgcgcgt gatccggccg
    34801 tccgcgctgg ccggcaacgg ccggcgtcgc acgctgctgc tgtccgcgac catgctggcg
    34861 gtgggcgtcc tggcggccca gctcacgctg ctgacccccg cctccacggc caccggcgtg
    34921 ctcgccgcct ccgccgcgct cgggctgggc gtcggcaccg cgtggctgct ccgagctctc
    34981 cgcccgaacc gtgcccgctc cgcggccgag gcgctcgccg gcctgctggc cccgacgctc
    35041 gacgacagct acaccctgct cctcgccccg acgcttccgg tgcgcgacgc cgcgcggctc
    35101 gacggggtgc tcgtgggccc tgccggcgtc cgcgtcctca ccgtccgcga ctgggacggt
    35161 cgctaccgcg tccgcgggcg gggctgggag ttcgacgcgc gcggccgacg cggctggatc
    35221 acgtgccgca ccaacccgag cttcgaggcc gcgtcgctgt ccgaggggtt ctcacgctgg
    35281 gcgagcgacg ccggcctggt cctccccgac gtcgcgctgc gaccggccat cgccttcccg
    35341 aaccgtcgat cgcggatcgt cctggaggag ccgatcgacg aggtcatcac caccgacaac
    35401 gccccgtggt gggcgaacac catcggccgc gtgcagcggc tggacccctc ctcggtcgcc
    35461 cgggtggtcg cggcggtcat ggaggccgcc gaggcgcgct cggccgtcgg ccgtcggccg
    35521 gccgcgcagg acgccggctg agctaaccct ccgcgccggg gtggccctgg ccgctgcgca
    35581 gccaggcctc ggccgtcgcc tcgtcgagga cctcggccag cagctcgtcg ggccgctcgt
    35641 cgtcggcgac gatcagcacc tgcgcccggt cgcgcgccag cgccaccacc gcgctctcgc
    35701 ggttcagctg cgggtgcgcg gcggacgtga tggccgcgtc cgtcatgggc aggaatcggt
    35761 cgggtacgtt caggaggccg tccatcggcc ggctgccctg cgggacgtag atcgtgccgg
    35821 tcagacggaa cggcggcagg cccagctgcg cgcgcacgcc gcgcttgggg atccgcatgt
    35881 ggtcgccact cccctgcacg gcaccctcgc ccggctggtc ggtgagcagg acgacgagga
    35941 cctcgttcag gggcaccagg gcctggggga cgcgctgcgg cggggcgggg tcaccgtgct
    36001 cgccgatggt cgcctcgtcg atcaccaggt gcgcgccggt gttctggttg aggatgtcgg
    36061 ccacgcgcgt gaagcgggtc ttcatgtggc ccgacacccg gtaggcgctg gtgtacgcct
    36121 cgatcgccac gtcgtggacg gtcccgaacc cctcgaaccc gaagccactc atggccgatc
    36181 tcctccgcga tgctgacgtc ctgtcgcgcc gatcctaccc gccgctactc gggcggcgcc
    36241 tccccggtcg ccgcgatctc ctccgtctcg ccgcgggcgc cggtcgccag caggatccgt
    36301 ccggatgcct gctcgccgaa gagggcctcc acggtcgcca acgtgtcgat gtcggcgggc
    36361 gtcttgtcgt cgcggatgcg gacgatccgc gggaagcgca tggcgaagcc ggaccggtgc
    36421 cgccccgaac gcatgatccg gtcgaaggcg atctcgacca ccacctcggg caccacggtc
    36481 cggtagcggc cgaagtcgcg cagggtgatg gccttgaagt gctcggtcat ctcggcgatc
    36541 tccgcatcgg tcaacccggt gtaggccttg ccgatggtga gcagccgcgc gtccggcgca
    36601 tcggtctcgc gcacggcgaa ggtgtagtcc gagaggacac cgcgacgctt gccatggccc
    36661 cactcgacgc cgaccacgac gcagtccagc gtggcgagcg ccttcttcag cttcagccac
    36721 ccgagccctc gacgccccgg ctggtagacg gaggcggggt ccttgaccat cagcccctcg
    36781 ttgtggcgct cgcgcgcgtc gtcgaaatgc cggtccacct ccttcgcgga ggcggcggtg
    36841 gccaggtgcg agagcagcac gcgctcgccg gtcacctcgg gaaggcccag gcgctcgagc
    36901 tcgccacgtc gctcgcggag cggcacctcg agcaggtcgc ggccgccgtg gtgaagcagg
    36961 tcgaaggcga ccagcaccac cgggacctcc tccaggagcg cctgcgacgg gcggacccgc
    37021 cccagccgcg tctggaggga cgcgaagtcc aggacggatc ctgcccgcca cgggaccagc
    37081 tcgccgtcga gcaccagcgg accgtcgccc gtgagcgcgg cgccggcctg cgcgatctcc
    37141 gggaacgagc gggtcacgtc gttgaggtca cggctgaaga ggcggacggt gccgtcagcc
    37201 tcgagatgga gctgcgcgcg gatgccgtcg tacttgtcct cgatccacgc ctcgtcaccg
    37261 acgcgacgca tgacctcggc cgcgtcggcc acgggcgagg cgagcatgaa gcggatgggg
    37321 cggcccacgt gcagcgcggc gccggccagg cggtcgtcgc gggccagcag cgcggtctcg
    37381 ccgatgtcgc cggtgagcat gtgcgcccgg cgaaccgcgt cgaggtcccg gtcgaacgcg
    37441 gccgccaccg cctcctccag cagcccctcg cgcagcccga tgcgcgtctc gcgcgagctg
    37501 atgcggccga tgaagcgggc ctcgtctccg gtggcccggc ggaacaggtc ggtgaagcgg
    37561 gtgacccgct cctcggccga tcccgccgcc gcgatggagg cgaacccgtc cgccacgtcc
    37621 gccacggtca gcggcgcgcc gcccgatggt gcgcgctcgc cgagcagctc cgcggcggcg
    37681 tcgccgaagt cggagtggcg caggtaggcg gtacgcagcc gctcctcgtc ggcgccgctg
    37741 gccgccgcga gcgccgcccc ctgctggacc cagcccatgc cgagcttggc ggtcgggtcg
    37801 gtgagcgggc ggccggcaaa gaaggtcgtc gccgccacca ggtcgcccgc cggcaggtca
    37861 cgcaggtagc cggccagcag gtcgcgcttg gcgttcttgc tgcgggtggc gccgaccgcg
    37921 tcggccgtcg cggccaggcg agcgaacggc gtggaggcgg cgcgcggcgg catcggccca
    37981 gtctacgcag cgcccggcca cggccgggct tgacccggag gcagccgggg cggatgctgc
    38041 cgcgcatggt cttcccgtgg cagaagcgca acgtgccgga cgagctcgcc gcccgcgtcc
    38101 caccggggca ggtgctgacc gagaagttcc cggtcctgca tttcgggcgc gttccggact
    38161 atggcgacct gtcgggctgg gacttccggg tgtggggtga ggtggacgag ccgttccgcc
    38221 tcaactgggc ggagttccgc aaccttccgg tggcggagct gacgaccgac atccactgcg
    38281 tcacgcgctg gtcgaagctc gacacgcggt gggagggcgt cgccttcagc catgtcgccg
    38341 aggtggccgt cgtgcgcccg agcgcccgct tcgtgatctt ccacgccgaa ggcggcttca
    38401 ccgccaacgt gccgctcgag acggccatgc gccccgaatg catcctggcg tggtcctacg
    38461 acgggcaacc gctgacggcg gagcacggct acccgctgcg ggcgatcgtc cccgggacct
    38521 acttctggaa gagcgcgaag tggctgcgcg gcgtcgagtt caccacggac gaccgcctcg
    38581 gcttctggga gcgcaacggg taccacaacg acgcggaccc gtggaaggag cagcgctact
    38641 cgcgctgacc ggagtcctcg ccgggctcgc cgggcatggt cttggcgcga gcgatgagct
    38701 cagcgacccg acgtccaccc ttgcggccga tctcgcggaa gtgctcgttc gagtaccgct
    38761 cgctgaccgc cttgccgccc ttggcgccga tctcctggaa gaactccggc ccgtagcggg
    38821 ctcgcgtggt cttgccaccc ttccgccccg cctcccgaac gctcatgttg ccgccggcct
    38881 tgccggccgc ggccgtcccg cccgtgttga cggtgttgct gccgttctgc tcggcgctgc
    38941 cgcgcttcgc catctggcgc tcctgttctg cgtgcttggt gctccgatcc ccccgctccc
    39001 cagccgaagc aagttgcgta cctgccgtcc ccacgggcga gcggcacgcg tcgagggccg
    39061 ggagcgcgac cgggctagaa tcagccgccc atgcacccga tgctccgcaa caccatcatc
    39121 ggcatcgtcg gcctgatcat cgccggcgcg ctggtggccc tcgccctcct cggcgacgat
    39181 tccgacctgt ccgtcctcgc catgctcgtg gccggcatcg tggccgtcgc catcggcctg
    39241 ttcgtgtacg cccagggctg ggtatggggc tcgcgcgccg cgcggcagcg cgccacgggc
    39301 cagtcgctcc tcatcgccgt cggcggcgga atcatggtgc tgatcgccgg cgtcgccatg
    39361 gccggcctgg tcatcctggt cctgctgttc tacctcggct gaccgccacg cctggccgat
    39421 cggccggccg cgacgccgct ggtaccatgc cgatgcgcgc gcgtgccccc atcgtctaga
    39481 ggcctaggac gccacccttt caaggtggag atcacgggtt cgaatcccgt tgggggcacc
    39541 actcctcaga tgtggttgat ctcgacccgg tccccgcagt tcgggcacca cagcaggagc
    39601 agcttcggat cctccggcaa cgggcgccac gagtagacgt tggcctcggt gatctgctcg
    39661 ccgcagcggg ggcactccga gccgaggtcc agccactgat cgcgcggcac gttctgccgg
    39721 cccatcagcg acgctccggt ggtggcggtg gcgcccatcc tggcttcggg ccggatggct
    39781 ccgacccgtc cggcgagtcg gccgcgacga tggcggcacg cgtctcctcc gccttcgccg
    39841 acggcctgat gagggggtac gacatggcgg cgtagccggg ccagctgccg acgtccccgt
    39901 cgcggatcat caccatcgtc ttggtgaacg cgccggtaac gtcgcgcacg gagcggtcca
    39961 ggacctggaa gtcgcgaccc gtcacgtggc gcagggccgg cggcagcgtc tcgaccaggt
    40021 aggcggggtt cgggttctcg gcgaacgcga gatccagcgc ctcgacgacc tgctcgtcgg
    40081 gcagcgaggt cggcagcggc cggctgatgt aggccatgcg cggatcgcgg ctgggtccct
    40141 tgaaggtgcg gacgagtgac ttgaacatcg gcgcggccga ttgtagcgcc ctcgaccgct
    40201 ccacccggcc ggcgccagcc ggccagcgcg ggcctggccc gcgcatcggt ccgtgcaacg
    40261 ccggttggta tactggcgtc ccctgtgagg aacccaaccg cggccccgcc gcgcgcagcg
    40321 atttggcgcg ccatcgccgc gcattcgacc ccgtaccaag gagcccatcg atcatcgtgc
    40381 gcgtgagaaa ggccgtcctg ccggcggcag gattcggcac ccgcttcctc ccagccagca
    40441 aggccgtccc caaggagatg atgccgctgg tcgaccgccc gatcatccag tacgcggtcg
    40501 aggaggcggt cgcctcgggc atcgagcaga tgatcatcgt catcgcgacg gggcgcagcg
    40561 cgatcgagga ccacttcgac tccaactcgg gcctcgagcg ctggctggag gaccgcggcg
    40621 acatcgagat gctgcgtcag atccggcgga tcagcgagtt cggcacggtc gcgttcgtcc
    40681 accagcgcga gcagctcggg cttggccatg cggtgctcat ggccaaggac ctggtcggcg
    40741 acgagccgtt cgccgtcctc ctctcggacg acgtgatgct caaccccggc ggagaaccgg
    40801 tcacgcgaca gctgatcgac gcgtacctgg cgcatcgcgg ctcggtgatc gccgtccagc
    40861 gggtcgatcg ccaggacgtc agccgctacg ggatcgtgga cccgctccac gaggaggggc
    40921 ggctggtcga gatccgcaac ctggtcgaga agccgaccgt cgaggaggcg ccatccgagc
    40981 tggcggtgct gggccgctac atcctcaccc cgaagatctt cgacgtgctg gagcacacca
    41041 cccgcggcgc gggcggcgag atccagctga ccgatgcgat ccggacgctg atcggcgaac
    41101 agcccgtgtt cggctaccag ttcgagggca cgcggcacga tgccggcacg cggctcgggt
    41161 ggctgaaggc caacgtggcg gtcgcgctcg agtccgacga ggccgacgaa ttccgaacct
    41221 acgtgcgcgg tctcgacctg cgagactgac gtcgtggaga tgatcggcag gctcatcagc
    41281 ggccggtacc gcctcatcgc cccgctcggc gagggcggca tggcgacgat ctggcgcgcc
    41341 gtcgacgagc agctcgaccg cgaggtggcc gtcaagatcc tgcggccgca gttcagcgcc
    41401 gatcccggct tcgccgcgcg cttcaagcag gaggcgcgcg ccgcaggtgg cctcagccac
    41461 ccgaacatcg tcagcgtcta cgactacggc accgacggcg ccgatggcga gcagtacatc
    41521 gtcatggagc tggtcgaggg ccgcgacctg gcctcgatca tctccgagcg cggcgcactg
    41581 tccgtcgacg actcggtgcg cgtcgcgatc gccgtggcca gcgcgctgga ggccgcgcac
    41641 cgcaagggca tcgtccaccg cgacgtgaag ccgggaaaca tcctgatcac cgatcacggt
    41701 gacgtgaagg tcaccgactt cgggatcgcc cgcgcggtca gcgaggcgtc gatgacggtc
    41761 accggcacga cgctcggatc ggtccactac ttcagcccgg agcaggcgcg cggggacgag
    41821 gtcaccggcg cgtccgacgt ctacgccctc ggcatcgtgc tgttcgagat gctgaccggc
    41881 cgccgtccct tcgagggcga ctcggccgcg gccgtggccc tcaagcggct caacgaggac
    41941 gcgccgaccc cctcgggcat cggccgcccg gtcccggccg gcctggaggc gatcctggcc
    42001 cgcgcgctgg cgcgcgagcc gatcgatcgg ttcccggacg ccggagccct ggcggaagcg
    42061 ctgcgcgtgt ggcgccgcga ccccgatgcc gtcccgggcg tcgtcccggc cagcgcgagc
    42121 accatggcgg gcgccgcggc cgccgcaccg gttccggccg gcacgccgcc cgccggcgag
    42181 ccgaccgtgt acgtcccgcc gcgcgttgcg cgacctgccg accgcgtccc ggtcgcgcca
    42241 ccgccgcgtc gcgccgacgt ctaccagcgg ccgcgcgacg aggggacgcc gtggtggatg
    42301 tggctgctgg cggtgctcgc cgtcttcctg ctcggcgcca tcggcttcct cgccgtccag
    42361 ctgctgggca gcggcacccc ggcggccacg cccagcccga ctgcggccac ggtcatcgtt
    42421 ccgacctgcg acgacgtgtc cctggccacc ctgcggcagc aggccgatga gctgggcgtg
    42481 accgtcgaca ccgagtcgga gccctccgag gacatcgacg agggccgcgt catccgctgc
    42541 gatcccgaga ccggccagcc ggtc
//
LOCUS       DASTYP010000244         6925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1085452, whole genome shotgun sequence.
ACCESSION   DASTYP010000244 DASTYP010000000
VERSION     DASTYP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085452"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFV59_10550"
     CDS             complement(<1..873)
                     /locus_tag="VFV59_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5326310.1"
                     /translation="MTDDQRWREHQDRWARERLDPALERAPERRPRFITQSGVEIERV
                     YSPADLHDPANDPDAGRPRYRFSPPPSEAAGWDEIDDLGLPGEPPFTRGIHPTGHRGR
                     LWTMRMFAGFGTAEDTNDRFKKLIAAGGTGLSIAYDMPTLYGYDHDDPMAEGEFGTCG
                     VAVSSLADMEILLDGLPVGEISTSMTINSPAAMIWAMYITAAEKMGVPRAQLAGTLQN
                     DILKEFIAQKEYIFPPAPSLKLVTDTVEWGTRELPRWNTISISGYHIREAGSTAVQEL
                     AFTIADGIAYVEDAL"
     gene            957..2981
                     /locus_tag="VFV59_10555"
     CDS             957..2981
                     /locus_tag="VFV59_10555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5326311.1"
                     /translation="MSEAFDFDAYLRLPRLSGLRVSPDGSRLVVTLGRPAPDGKKMHS
                     SIWELDATGTDRPRRLTRSAPGESAAAFARDGSLLFTSSRPDPDRKPDDDDEEVSGLW
                     LLPGNGGEARLLARPSGGVDAVVAARASDVIVYAGSIFPGADANADKERHDARKKAGV
                     EALLFDTFPIRHWDAYHGPRERHLFAAELPPGEDRIEERRDLTPDAGLVSLVEQQFDI
                     TPDGTTVVTGVLTPDDIGRTDFGIDVIDRSSGDRRRLTADDAWYSDPAISPDGRSVVC
                     VRMQKDTPERIGDHTLWLIDVASGEGRDLTPDLDLWPHGAVWAPDGSAVYFVTDYRGG
                     GAVHRWDAATGDVTRLADDGSFTDLAVGSDGTVYALRSSYAAPPHPVRIGSTANAGSE
                     PIRSFAELDPEALADLPGRVERLSANAADGIEIGSWLVEPTEASAESPVPLVVWVHGG
                     PVGSWNAWSWRWNPHLLAAQGYAVLLPDPAISTGYGHAFIQRGWGRWGAEPYTDVMAA
                     VDSALQRPELDAEKTALMGGSFGGYMANWVAGNTDRFRGIVTHASLWELRGFHGTTDY
                     GPDWEREFGDPYADPSRYEEWSPSRLLSKIRTPMLVIHGERDLRVPISEALRLWTDLR
                     RHDVPARFLYFPDENHWILKPQNARLWYATVLSFLGEVLRGEEFTRPPLL"
     gene            complement(3173..4117)
                     /locus_tag="VFV59_10560"
     CDS             complement(3173..4117)
                     /locus_tag="VFV59_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEU5326312.1"
                     /translation="MTTTTSPATPARDLLAELEPAAERLMNRHLGMAREWFPHDYIPY
                     SLGRDYDKEPWTPDQPRLSGVAQIAFEVNLLTEDNLPSYHREIHRMFGRGDGAWINWV
                     NRWTAEEGRHAIVLRDYLVVTRNIDPYALERSRMNQLQIAYDHDDGGNVLRGFAYVAF
                     QELATRISHRNTGRYSSDPVADRIMARIAADENLHMVFYRDILAEAMALEPSAAVCAI
                     ADEIMAFEMPGAGMVNFTRKAAQMAKAGIYDLRIHHDEVVWPLLRHWGVFEATGLNAE
                     AERRRSELRTFLDSLDAQASRFEARRAQGLQREAERAG"
     gene            complement(4233..4646)
                     /locus_tag="VFV59_10565"
     CDS             complement(4233..4646)
                     /locus_tag="VFV59_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326313.1"
                     /translation="MNEQFPLRSDRPWRWPLRLIGVRRGRAQVELTEDGRLRATFGRL
                     SVETAVDNVCGYRITGPYHWWKAIGPRGSLVDKGFTFGTSARDGVCLCFREWVPTRYV
                     RGGRMEALTVTVDDPEALAAALERRGIRGEDQRRR"
     gene            complement(4688..5947)
                     /locus_tag="VFV59_10570"
     CDS             complement(4688..5947)
                     /locus_tag="VFV59_10570"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5326314.1"
                     /translation="MGIGSRTGSLWATTQNELDLAAERLQLDDAMHRVLRLPKRELTV
                     NFPVTHDDGHVDVYTGYRVHHNVNRGPANGGIRYVPNLELDDVRALAMLNSWKAALVR
                     IPFGGAAGGVRVDPRRLSINERQGLTRRYATEIAPLLGRERDIPAPDVNTGSQTMAWM
                     MDTFSMHSGHTTPASVVGKPLAVGGTRGRRSATARGAFRCIVAAARAGDVALDGARVA
                     IQGFGRVGMTVADELVRAGARVVALADDRDAVAAPGGIEIGRAVDWMREQDAVRGMPN
                     TEPMGKADIFALDVDVLVLAGLEGELTDANAGSVRARIVAEIANGATTPDADAILDAA
                     RIALIPDILCTAGGTVLGYFEWVQDMQAFFWSTDEVTRQLDRVMDEAMSGVQAMADAQ
                     KVDLRSAATMVAVARVAEATSLRGLYP"
     gene            6081..>6925
                     /locus_tag="VFV59_10575"
     CDS             6081..>6925
                     /locus_tag="VFV59_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="phosphotriesterase homology protein; PhP; YhfV;
                     member of a family of proteins related to
                     phosphotriesterase (PTE); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HEU5326315.1"
                     /translation="MAPRVMTVSGPIPPEKLGFTLPHEHVGIALWHVDRRWDYWELTP
                     DDDLILEELRELRRRGGGTLVDLTLDGVGRDPHRLRRLASATGLNIVMGAGWYRGAHY
                     PAELLVERRTVDELAGLIVREFEEGVGDTGIRPGIIGEIGTDKPWVSPAEERVHRAAA
                     RASLRTGLAITTHGVQSPVGLSQLKIFTEEGVDPSRVVIGHADSYHDLDFYLRVLDAG
                     ANLEFDFLGHRFGVEEHLEPRLVETIVELLEREYGPQLLLSQDVCHNRQLKANGGFGY
                     VYLHQ"
BASE COUNT          952 a         2428 c         2509 g         1036 t
ORIGIN      
        1 cagggcgtcc tcgacgtagg cgatcccgtc ggcgatggtg aaggcgagct cctggaccgc
       61 ggtggagccg gcctcgcgga tgtggtagcc cgagatcgag atggtgttcc agcgcggcag
      121 ctcgcgcgtg ccccactcga ccgtatcggt caccagcttc agggacggcg ccggcgggaa
      181 gatgtactcc ttctgggcga tgaactcctt caggatgtcg ttctggagtg tccccgccag
      241 ctgggcgcgc ggcacgccca tcttctcggc ggccgtgatg tacatcgccc agatcatggc
      301 ggcgggggag ttgatggtca tcgaggtgga gatctcgccg acgggcagtc cgtccagcag
      361 gatctccatg tcggccagcg aggaaaccgc gacgccgcag gtcccgaact cgccctcggc
      421 catcgggtcg tcgtggtcgt agccgtagag cgtcggcatg tcgtaggcga tcgacagccc
      481 ggtgcccccg gccgcgatca gcttcttgaa gcgatcgttc gtgtcctcgg ccgtgccgaa
      541 gcccgcgaac atgcgcatgg tccagaggcg gccgcgatgg ccggtcgggt ggattccgcg
      601 cgtgaagggc ggctcgcccg gcagccccag gtcatcgatc tcgtcccagc cagcggcctc
      661 ggacggcggg gggctgaacc gatagcgagg ccggccagca tcggggtcgt tggccggatc
      721 gtgcaggtcg gccggcgagt acacccgctc gatctccacg cccgactggg tgatgaagcg
      781 cggacgccgc tccggcgcgc gctcgagggc ggggtcgagg cgctctcgcg cccatcggtc
      841 ctggtgctcg cgccagcgct gatcgtcggt catggcgcgc agtgtgcggg caagaccgat
      901 gccgcggcaa ctcggcgcgg ctcggcggcg agcgcccgcg gcgtaggatg cgggccatga
      961 gcgaagcctt cgatttcgac gcctacctgc gtctgccccg cctctcgggc ctccgcgtct
     1021 caccggatgg cagccggctc gtcgtcacgc tgggacgccc cgcgccggac ggcaagaaga
     1081 tgcacagctc gatctgggag ctggacgcca ccggcaccga tcggccgcga cggctcactc
     1141 ggtccgcacc gggcgagagc gccgccgcgt tcgcgcggga cgggtcgctg ctcttcacct
     1201 cgtcccggcc ggaccccgac cgcaagcctg acgatgacga cgaggaggtg agcgggctct
     1261 ggctgctgcc cggcaacggt ggcgaggcac ggctgctggc gcggccgtcc ggcggcgtgg
     1321 acgccgtggt ggcggcgcgc gcctccgacg tcatcgtcta cgccggctcg atcttccctg
     1381 gcgccgacgc gaacgccgac aaggagcgtc acgacgcgcg caagaaggcc ggcgtggagg
     1441 ccctgctgtt cgacaccttc ccgattcgac actgggacgc gtaccacggt ccgcgcgagc
     1501 ggcacctgtt cgcggccgag ctgccgccgg gggaggaccg catcgaggag cgtcgcgacc
     1561 tgacgcccga tgccgggctt gtgtcgctgg tcgagcagca gttcgacatc acgcccgatg
     1621 gcacgaccgt cgtcaccggt gtcctgacgc cggacgacat cgggcgcacc gatttcggca
     1681 tcgatgtcat tgatcgatcg agcggtgacc gtcggcggct gaccgctgac gacgcctggt
     1741 actccgaccc ggccatcagc cccgacgggc ggtcggtcgt ctgcgtgcgg atgcagaagg
     1801 acacgccgga gcgcatcggc gaccacacgc tgtggttgat cgacgtggcc agcggcgagg
     1861 ggcgcgacct gaccccggac ctggacctct ggccgcatgg ggcggtgtgg gcgccggacg
     1921 ggtcggcggt ctacttcgtc accgactacc gcggtggcgg agcggtgcat cgctgggacg
     1981 cggcgaccgg cgacgtcacg cgcctcgccg acgacggctc cttcaccgat ctcgccgttg
     2041 gctcggacgg gacggtgtac gcgctgcgct cgtcgtacgc cgcgccgccg cacccggtgc
     2101 gcatcggttc gacggcgaac gccggcagtg agccgattcg ctccttcgcc gagctcgatc
     2161 ccgaggcgct cgccgacctc ccgggtcgcg tcgagcgcct gagcgcgaac gcggccgacg
     2221 gcatcgagat cggttcctgg ctggtcgagc ccacggaggc gtcggcggag tcaccggtcc
     2281 cgctggtggt ctgggtccac ggcgggccgg tgggcagctg gaacgcgtgg agctggcgct
     2341 ggaacccgca cctgctcgcg gcccagggct acgccgtgct gctgccggac ccggccattt
     2401 cgaccggcta cggccatgcc ttcatccagc gcggctgggg tcgatggggc gccgagccgt
     2461 acaccgacgt catggccgca gtcgactcgg cgctgcagcg cccggagctg gacgccgaga
     2521 agacggcgct gatgggcggc tcgttcggcg gctacatggc caactgggtc gcgggcaaca
     2581 ccgaccggtt caggggcatc gtcacccacg ccagcctgtg ggagctgcgc ggcttccacg
     2641 ggaccaccga ctacgggcct gactgggagc gtgagttcgg cgacccgtac gccgatccat
     2701 cccgctacga ggagtggtcg ccgtcacgcc tgttgtcgaa gatccgcacg ccgatgctgg
     2761 tcatccacgg cgagcgcgac ctgcgcgtgc cgatcagcga ggcgctccgg ctctggacgg
     2821 acctgcgccg gcatgacgtg ccggcccggt tcctctattt cccggacgag aaccactgga
     2881 tcctgaagcc gcagaacgcc cgcctctggt acgccaccgt gctgtcgttc cttggcgaag
     2941 tgctgcgcgg cgaggagttc acgcgcccac cgctgctctg accgacccgg gctcgcggcg
     3001 cgtggcgcgg cacgcggcgt gcggaaggtg acgacatgct tgcgctgatt cgtcgcttcg
     3061 tcgtcggctg gcttctcctg cgcctcgtgc ggcggctcgc cgggcggagc gatgggcggc
     3121 gacggccgac gcgctgaggg gacccgagcg tcagcaggac ggtgcagcca ggttagcccg
     3181 cgcgctcggc ctcgcgctgc aggccctggg cgcgccgtgc ctcgaaccgc gacgcctggg
     3241 catcgagtga gtcgaggaag gtccgcagct cgctgcggcg tcgctccgcc tcggcgttca
     3301 ggccggtggc ctcgaagacg ccccagtggc gcagcagtgg ccacacgacc tcgtcgtggt
     3361 gaatgcgcag gtcgtagatc ccggccttcg ccatctgcgc ggccttgcgc gtgaagttga
     3421 ccatcccggc gcccggcatc tcgaaggcca tgatctcgtc ggcgatggcg cacaccgcgg
     3481 cggagggctc gagagccatc gcctcggcga ggatgtcgcg gtagaacacc atgtgcaggt
     3541 tctcgtcggc cgcgatgcgg gccatgatcc gatcggccac cgggtcgctg gagtagcggc
     3601 cggtgttgcg gtggctgatg cgcgtcgcga gctcctggaa ggcgacgtag gcgaacccgc
     3661 gcaggacgtt gccgccatcg tcgtggtcgt aggcgatctg gagctggttc atgcgggacc
     3721 gctccagcgc atacggatcg atgttgcggg tgacgaccag gtagtcgcgc agcacgatcg
     3781 cgtggcgccc ctcctcggcg gtccatcgat tcacccagtt gatccacgcc ccgtcgccgc
     3841 gcccgaacat gcggtggatc tcgcggtggt agctggggag gttgtcctcg gtcagcaggt
     3901 tgacctcgaa cgcgatctgg gcgacccccg agagacgcgg ctggtccggc gtccacggct
     3961 ccttgtcgta gtcccggccc agcgagtagg ggatgtagtc atgcgggaac cactcgcggg
     4021 ccatgcccag gtggcggttc atcaggcgct ccgcggccgg ctccagctca gccaggaggt
     4081 cgcgtgccgg ggtcgccggc gaggtggtcg tcgtcatggt gctccgagta cgtccaggtg
     4141 gcgcaccgcg gatgacgacg cgcgaggaag gtgcccccgc gtggacatgg ttgcacgaac
     4201 cgggccgcgc cgcccccgcg gtgccctcga cctcagcggc ggcgctggtc ctcgccgcgg
     4261 atgccgcgcc gctccagcgc cgcggcgagg gcctcaggat cgtcgaccgt cacggtgagc
     4321 gcctccatcc ggccgcctcg cacataacgg gtcggcaccc actcgcggaa gcagaggcag
     4381 acgccgtcgc gggcgctggt cccgaaggtg aagcccttgt ccaccagcga cccgcgaggc
     4441 ccgatggcct tccaccagtg gtacgggccg gtgatgcggt agccgcagac gttgtcgacg
     4501 gccgtctcga cggagaggcg cccgaaggtg gcgcgcaggc ggccgtcctc ggtcagctcg
     4561 acctgcgccc gaccccgtcg cacgccgatc agccgcaacg gccagcgcca cggccggtcg
     4621 gatcggagag ggaactgctc gttcacggcg tggggtacgg gcagaccgct cggtcggacc
     4681 agcccgacta ggggtacagc cctcgcagcg aggttgcctc ggcaacccgc gcgaccgcga
     4741 ccatggtggc cgcgctgcgg aggtcgacct tctgcgcgtc ggccatcgcc tggacgccac
     4801 tcatggcctc gtccatcacc cggtcgagct gccgcgtgac ctcgtcggtg gaccagaaga
     4861 aggcctgcat gtcctgcacc cactcgaagt agccgaggac cgtgccgccc gccgtgcaca
     4921 ggatgtcggg gatgagggcg atgcgggccg cgtcgaggat cgcatccgca tcgggggtcg
     4981 tggcaccgtt cgcgatctcg gccacgatcc gcgcccggac cgaccccgcg ttcgcatcgg
     5041 tcagctcgcc ctccaggccc gcgaggacca ggacgtccac gtccagcgcg aagatgtcgg
     5101 ccttgcccat cggctcggtg ttcggcatgc cgcgaacggc gtcctgctcg cgcatccagt
     5161 ccaccgcacg tccgatctcg atgccgcccg gcgcggccac cgcgtctcgg tcgtccgcca
     5221 gcgccaccac ccgcgcgccg gctcggacga gctcgtcggc gaccgtcatg cccacgcggc
     5281 cgaacccctg gatcgcgacc cgcgcgccgt ccagcgcgac gtcgcccgcc cgggccgccg
     5341 cgacgatgca gcggaaggcg ccgcgcgcgg tggcgctgcg tcggccgcgc gtcccgccga
     5401 cggcgagcgg cttgccgacc accgacgcgg gcgtcgtgtg gcccgagtgc atcgagaagg
     5461 tgtccatcat ccaggccatg gtctgcgagc cggtgttcac gtcgggcgcg gggatgtccc
     5521 gctcgcggcc cagcagcggc gcgatctccg tggcgtatcg gcgcgtgagc ccctggcgct
     5581 cgttgatgct gagccggcgc gggtcgacgc ggacgccgcc ggccgcgccg ccgaacggga
     5641 tgcggaccag cgccgccttc cacgagttca gcatggcgag ggcacgcacg tcgtcaagct
     5701 ccaggttcgg gacgtagcgg atgccgccgt tggccgggcc gcgattgacg ttgtggtgga
     5761 cgcggtagcc ggtgtagacg tccacgtggc cgtcgtcgtg ggtcaccggg aagttcaccg
     5821 tcagctcccg cttcggcagc cgaagcacgc ggtgcatcgc gtcgtcgagt tgcagccgtt
     5881 cggcggcgag gtcgagctcg ttctgggtcg tggcccacag gctgccggtg cgagagccga
     5941 ttcccatgcg cggatgctac cccgccaccg ccatgcgacg aggcgacggt ttgcccgcgt
     6001 ggcaacatgg cgtcctacgc atcaccgatg cgccggcgtc ccgatgaacg gcgaccggcg
     6061 ccgaccgagg aggacccgcg atggcgcccc gcgtcatgac cgtctccgga ccgatccctc
     6121 ccgagaagct cggcttcacg ctcccgcacg agcacgtcgg catcgcgctc tggcacgtcg
     6181 accggcgctg ggactactgg gagctgactc ccgacgacga cctcatcctc gaggagctgc
     6241 gcgaactgcg gcgccgcggg ggcgggacgc tggtcgacct cacgctcgac ggcgtcggcc
     6301 gcgatccgca tcggctccgg cgtctcgcca gtgcgaccgg cctgaacatc gtgatgggcg
     6361 cgggctggta ccgcggcgcg cactacccgg cggagctcct cgtggagcgg cggaccgtcg
     6421 acgagctcgc cggcctgatc gtgcgggagt tcgaggaggg ggtgggggac accggcatcc
     6481 ggcccgggat catcggcgag atcggtaccg acaagccgtg ggtctcgccc gccgaggagc
     6541 gcgtgcatcg cgcggcggcg cgcgcctcgc tgcggaccgg cctcgccatc accacccacg
     6601 gcgtccagtc gccggtcggg ttgtcgcagt tgaagatctt caccgaggag ggcgtcgatc
     6661 cgtcgcgtgt cgtcatcggt catgccgact cgtaccacga cctcgacttc tacctgcggg
     6721 tgctggatgc cggcgccaac ctggagttcg acttcctggg gcatcggttc ggggtcgagg
     6781 agcacctgga gccgcggctg gtggagacga tcgtggagct gctggagcgc gagtacgggc
     6841 cgcagctgct gctctcccag gacgtctgcc acaaccggca gctcaaggcc aacggcgggt
     6901 tcgggtacgt ctacctgcac cagca
//
LOCUS       DASTYP010000245         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1011399, whole genome shotgun sequence.
ACCESSION   DASTYP010000245 DASTYP010000000
VERSION     DASTYP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011399"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="ftsZ"
                     /locus_tag="VFV59_10580"
     CDS             complement(<1..716)
                     /gene="ftsZ"
                     /locus_tag="VFV59_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5326316.1"
                     /translation="MPLRSDSENFALIKVIGVGGGGSNAVNRMIRAEMMGVEFISCNT
                     DAQALLQSDAPHKIRIGDKITRGLGAGGDSAIGARAAEEDSEKIAQALADSDMVFITA
                     GLGGGTGSGAAPVVAQLAKEAGALTIGVVTKPFTFEGAKRKLVAEKAAEELKAQVDTL
                     ITIPNDRLKDVVQKNTSIIDAFRVVDDVLRQGVQGISDIITMPGLINLDFADVRAIMK
                     DAGSALMGIGRGSGENRAVEA"
     gene            925..1389
                     /locus_tag="VFV59_10585"
     CDS             925..1389
                     /locus_tag="VFV59_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5326317.1"
                     /translation="MSELTNEEVARRYFEAATRNDLDALEALRADDWELRWPATGELV
                     RSSAAYRAIHQRFPGGYPHIDALRIIGSEDRYVVTPANTVVRLAGSGDVWIGQGRLRY
                     GDGSEWYFVKLVELRDGRIHRETDYWSPVTDPPAWRAGMADRLPTTDDPHRA"
     gene            1539..1943
                     /locus_tag="VFV59_10590"
     CDS             1539..1943
                     /locus_tag="VFV59_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326318.1"
                     /translation="MVVDDRAEALELLEEILSLEHGMRVVQRLSATVDDLAAEQPDLL
                     VLDLWVGPAQRANGWSLACAARRDPRLRDVGIVLTSADRRSLDRFADPAQRNDVVLLS
                     KPFGLDDLHHAVSSALARRSHLSRVAPGAPGP"
     gene            1948..2301
                     /locus_tag="VFV59_10595"
     CDS             1948..2301
                     /locus_tag="VFV59_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326319.1"
                     /translation="MAEDSIADLVRRIRRAALDARTLSVTLESSLATLHEIPSMSGDE
                     RMDARRRWIGEVRADLDRVERLSRKHHRLVEDIAARCGPLPVEAKARLDAELADADAR
                     IHAARAHLQRAANHG"
     gene            complement(2318..3559)
                     /gene="ftsA"
                     /locus_tag="VFV59_10600"
     CDS             complement(2318..3559)
                     /gene="ftsA"
                     /locus_tag="VFV59_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU5326320.1"
                     /translation="MEREAVLVGIDAGTTKVTTLIGEVGRTGDVNVIGYGIAPSVGMK
                     KGMVANIDQTVNSIASSVEKAERLSGYKISSAFVGIGGSHISSQNSRGVVAVSGHKRE
                     VSREDVARATEAAKAVQVPSNREILHVVPRGYIVDGQEGIKDPLGMSAVRLEVETHIV
                     AGAATSVQNLSKCVSSAGVQIDEMVISSLAAAEATLTDTEKELGVLVADIGGGTTDIA
                     IFVDGAVYHTAVLPVGGINVTNDVAIGLRTSLNLAEEIKIKHGTANLAEVEPEELLNI
                     AVLGEAAGQTIQRRKLCEIIEARMGEIYSLIREEVKRSGHAGMLPAGAVLTGGGARIA
                     GADVLARETLEMPVRIGSPQGVGGLMDQIGNPAFSSSIGLLLWGAHHLGDEPIGYGGG
                     GGNGVNPLSRIGEWIRNLFPG"
     gene            complement(3611..3997)
                     /locus_tag="VFV59_10605"
     CDS             complement(3611..3997)
                     /locus_tag="VFV59_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEU5326321.1"
                     /translation="MTSTPIGGPWPTEILASGPSHMWLPLVGLALGIALGLVLNVNVS
                     PDLARYSAVAILAGLDSVLGAIRAELDAHYDNRIFLSGFVTNALVAVALTFVGDRLGI
                     DLYLVALFAFGLRIFQNVALIRRHFL"
     gene            complement(3994..>4580)
                     /locus_tag="VFV59_10610"
     CDS             complement(3994..>4580)
                     /locus_tag="VFV59_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326322.1"
                     /translation="TDALQRRLERIPSIADATVRASITGRLEATVIEREAAFVWATNF
                     GRFLGAADGTVFASIPNADPMPDALVGLPLITDNRFAARVVSVGDRIPDALLTTALRL
                     EALDPALLGSSATSFSVSVDDDYGFRLTSAEPAWEIALGVYGLDPTETREEMDARIER
                     QVTAVRTLFASRPEAEIGWVDVRNPGKVYFRAKG"
BASE COUNT          662 a         1635 c         1582 g          701 t
ORIGIN      
        1 gcctcgaccg cgcggttctc gccgctccct cggccaatcc ccatgagcgc cgatcccgcg
       61 tccttcatga tcgcgcggac gtcggcgaag tcgaggttga tgagtccggg catcgtgatg
      121 atgtcgctga tcccctggac gccctggcgc aggacatcgt cgacgacccg gaaggcgtcg
      181 atgatcgagg tgttcttctg gacgacgtcc ttcaggcgat cgttcgggat cgtgatcagg
      241 gtgtcgacct gggccttcag ctcctcggcc gccttctcgg cgaccagctt gcgcttggcg
      301 ccctcgaacg tgaacggctt ggtcacgacg ccgatcgtga gggcgccggc ctccttggcc
      361 agctgcgcga cgacgggggc cgcgccggag ccggtcccgc cgcccagccc ggcggtgatg
      421 aacaccatgt ccgagtccgc gagggcctgg gcgatcttct ccgagtcctc ctcggccgcc
      481 cgggctccga tcgcgctgtc cccgccggca ccgaggccgc gggtgatctt gtcgccgatc
      541 cggatcttgt ggggcgcgtc ggactggagg agggcctggg cgtccgtgtt gcaggagatg
      601 aactccacgc ccatcatctc ggcccggatc atccggttga cggcgttgct gccgccacca
      661 ccgacgccga tgaccttgat gagcgcgaag ttctcggagt ccgagcggag cggcatgtcg
      721 ggtgtcccct tgacgagcag agtcggtgtg ctggttgatc aacccgaggg cttcgcgggt
      781 ttgaccgatt ctaccgatcc gcgtatgacg ccgtgcgacg ttttccacaa tcggaggggc
      841 acttatccac aggtcggcaa cgccgacgtg gacgatcgcg acctctgtcc accgggagga
      901 tgcgggtgta cgctcgcccg cagcatgagc gagctgacga acgaggaggt cgcgcgccga
      961 tacttcgagg ccgcgacccg gaacgacctc gatgcgctcg aggcactgcg cgccgacgac
     1021 tgggagctgc gctggccggc cacgggcgag ctggtccgct cgagtgccgc ctaccgagcc
     1081 atccaccagc ggtttcccgg cggctacccg cacatcgacg cgctgcggat catcggctcg
     1141 gaggaccgct acgtcgtcac gccggcgaac acggtggttc ggctggcggg cagtggcgat
     1201 gtctggatcg gccagggacg cctgcgctac ggcgacggct ccgagtggta cttcgtcaag
     1261 ctcgtcgagc tgcgcgacgg ccgcattcac cgcgagaccg actactggtc acccgtcacg
     1321 gatccaccgg cctggcgcgc cgggatggcg gaccggctgc cgaccacgga cgacccgcac
     1381 cgcgcctgac cgaccgcgtc cccgcttccc ggcgacgcac ctgcggcggc ggcttggacc
     1441 ggaacttgcg gcgtccgggc ggtcgaagga ggtcgaggac ggagaacgag atgcgcgacg
     1501 caccggtgcc gccaggatca tcggggaggc gccgagccgt ggtcgtcgac gaccgcgccg
     1561 aggccctcga gctcctcgag gagatcctct ccctggagca tgggatgcgc gtcgtccagc
     1621 gcctgagcgc caccgtcgac gatcttgccg ccgagcagcc ggacctcctg gtcctcgacc
     1681 tgtgggtcgg gccggcgcag cgcgccaacg gatggtccct ggcctgcgcc gcacgacggg
     1741 acccgcgcct gcgggacgtc ggcatcgtcc tcacctcggc cgaccggcgc agcctcgacc
     1801 ggttcgccga tcccgcgcag cgtaacgacg tggtcctgct gtccaagccc ttcggcctgg
     1861 acgatctgca ccacgcggtc agcagcgcgc ttgcccgacg ctcgcacctg agccgggtgg
     1921 cgccgggcgc gcccggcccc tgaggccatg gccgaggact cgatcgccga cctggtccgt
     1981 cgtatccgcc gcgccgccct cgatgcccgc accctgtccg tcacgctcga gtcgagcctg
     2041 gcgacgctcc acgagatccc cagcatgtcc ggcgacgagc gcatggacgc gcgccgtcgg
     2101 tggatcgggg aggtgcgggc cgacctggac cgcgtggagc gcctgagccg caagcatcac
     2161 cgcctggtgg aggacatcgc cgcccgctgc gggccgctgc cggtggaggc aaaggcacgc
     2221 ctggacgcgg agctggccga cgccgatgcg cgcatccacg cggcacgcgc gcacctgcaa
     2281 cgcgccgcga accacggcta gcggggcagc cggccgatca gccggggaac aggttccgga
     2341 tccactctcc gatgcgcgac agcgggttga cgccgttgcc gcccccgccg ccgtagccga
     2401 tcggctcgtc gcccaggtgg tgcgcgcccc acagcagcag gccgatggag ctcgagaagg
     2461 ccgggttgcc gatctggtcc atcaggccgc ccaccccctg cggtgagccg atgcggacgg
     2521 gcatctccag cgtctcgcgc gccaggacgt ccgccccggc gatgcgcgct ccgccgcccg
     2581 tcagcaccgc accggcgggg agcatcccgg catggccgga gcgcttcacc tcctcgcgga
     2641 tgaggctgta gatctccccc atccgggcct cgatgatctc gcacagcttg cggcgctgga
     2701 tggtctggcc cgcggcctcg cccaggaccg cgatgttcag cagctcctcg ggctccacct
     2761 cggccaggtt cgccgtgccg tgcttgatct tgatctcctc ggccaggttc aggctggtcc
     2821 gcagcccgat ggccacgtcg ttggtgacgt tgatgccgcc gacgggcagg acggcggtgt
     2881 ggtacacggc gccgtccacg aagatcgcga tgtcggtggt gcccccgccg atgtcggcga
     2941 ccagcacgcc gagctccttc tcggtatcgg tcagggtcgc ctcggccgcg gccaacgagc
     3001 tgatgaccat ctcgtcgatc tgcacgccgg cgctcgagac gcacttgctc aggttctgca
     3061 ccgaggtggc cgcaccggcc acgatgtgcg tctccacctc gaggcggacc gcgctcatgc
     3121 ccagcgggtc cttgatgccc tcctgcccgt ccacgatgta gccgcgcggc acgacgtgca
     3181 ggatctcccg gttggagggc acctggaccg ccttggccgc ctcggtggcg cgggcgacgt
     3241 cctcgcggct cacctcgcgc ttgtgaccgg acacggcgac cacgccgcgc gagttctggc
     3301 tgctgatgtg gctgccgccg atgccgacga acgccgagct gatcttgtag ccggacagcc
     3361 gctcggcctt ctcgacgctc gacgcgatcg agttcacggt ctggtcgatg ttggcgacca
     3421 tgcccttctt catgccgacc gacggcgcga tgccatagcc gatgacgttc acgtcaccgg
     3481 tgcggcccac ctcgccgatg agcgtcgtga ccttggtcgt ccccgcgtcg atgccgacga
     3541 ggaccgcttc gcgttccaag ctacctcctg tgagcggcgc cccggagcgc gccatgagtg
     3601 ccgtcggagc ctacaggaag tgacgccgga tgagggccac gttctggaag atgcgcaggc
     3661 cgaaggcgaa cagcgcgacc aggtagagat cgatccccag ccggtcgccg acgaacgtca
     3721 gcgcgaccgc gaccagggcg ttggtgacga atcccgagag gaagatccgg ttgtcgtagt
     3781 gcgcgtcgag ctccgctcgg atggccccca gcaccgagtc caggccggcc aggatggcca
     3841 cggccgagta gcgcgccagg tccgggctca cgttgacgtt gagcaccagc ccgagcgcga
     3901 tcccgagcgc aaggccgacg agcggtagcc acatgtggga gggtcccgaa gcgaggatct
     3961 cggtgggcca ggggccgccg atcggcgtgg acgtcacccc ttggcacgga aatatacctt
     4021 tccggggttg cgcacatcca cccacccgat ttcggcttcg ggccggctgg cgaacagcgt
     4081 ccgcaccgcg gtcacctggc gttcgatccg cgcgtccatc tcctcgcgcg tctcggtcgg
     4141 atccagtccg tagacgccga gcgcgatctc ccaggcgggc tccgccgacg tcaggcggaa
     4201 gccgtagtcg tcgtcgacgc tcacgctgaa cgaggtcgcc gaggatccga gcagcgccgg
     4261 gtccagcgcc tccaggcgca gcgccgtggt caggagggca tcggggattc gatcgccgac
     4321 cgatacgaca cgcgccgcga accggttgtc ggtgatcagc ggcaggccga cgagggcgtc
     4381 gggcatcgga tccgcgttcg ggatcgacgc gaacaccgtg ccgtccgccg caccgaggaa
     4441 ccggccgaag ttcgtggccc acacgaacgc cgcctcgcgc tcgatgacgg tggcctcgag
     4501 ccgcccggtg atgctggcgc gcaccgtcgc atcggcgatg gacgggatgc gctccagtcg
     4561 ccgctgcagc gcgtcggtgt
//
LOCUS       DASTYP010000246        11178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_64284, whole genome shotgun sequence.
ACCESSION   DASTYP010000246 DASTYP010000000
VERSION     DASTYP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64284"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFV59_10615"
     CDS             <1..261
                     /locus_tag="VFV59_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5326323.1"
                     /translation="KNVAEGINASLSPEMGVLLITHYQRMLNYVKPQYVHVLMNGRIV
                     MSGGEELSHQLEANGYDWVREQVGLPAGIGDEAEAPEPVGSH"
     gene            295..1551
                     /locus_tag="VFV59_10620"
     CDS             295..1551
                     /locus_tag="VFV59_10620"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEU5326324.1"
                     /translation="MTVDQAPAGLEGATLRADFPVFDRPHRSGRPLVFLDSAASAPKP
                     RVVIEALADTYAHHYANVHRGLYELSEDATARYEAARRAVARFIGAPSEREIVFVRNA
                     TEAINLVAYSWARNNLGPGDLVVSTQMEHHANLVPWQQVAAATGATLAHVRITDDGRL
                     DRDDLRAQLARGPRLLAFAHVSNALGTINDVAEITRLAHEAGALVLLDAAQSVPHLPV
                     SVAELDVDFAVLSGHKMLGPSGIGALWARRELLDGMPPFLTGGSMITRVSLTEAEWNE
                     VPWKFEAGTPAIAEAVGLHAAIGYLEALGMERVRDHERHLFTRAWEALGKLPGVRRFG
                     PSDPDQHAGLISFDVAGVHPHDVATILDAEGVAVRAGHHCAQPVMERFDVPATTRASF
                     NVFNDVDDIDRLTDAIRAVQRIFGVA"
     gene            1551..1985
                     /locus_tag="VFV59_10625"
     CDS             1551..1985
                     /locus_tag="VFV59_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU5326325.1"
                     /translation="MDNLYRDFILEHYRNPHNRGYLHPHDLHFADANPTCGDEMSMTL
                     RLDADHQQVTDVAFEGRGCAISQASASLLTDELRGQSLDELRAMDPRDVLDSLGVPIG
                     PARFKCALLSYKVLQGALRGAEVQWPEEAEVLGVANEGGSAP"
     gene            1982..3055
                     /gene="moeB"
                     /locus_tag="VFV59_10630"
     CDS             1982..3055
                     /gene="moeB"
                     /locus_tag="VFV59_10630"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEU5326326.1"
                     /translation="MTWREVGAEEAVSLAQAGTTVIDVREATEWRAGHVAGAVHVPLG
                     EVGDRVPAIVPDRDAPLLVYCAVGVRSAHAAEWLSRAGYSRVASLRASLDDWRRAGGG
                     WVAEPSMLDEHARRRYARQIPIPEVGEEGQRRLLDSRVLLVGAGGLGSPVALYLAAAG
                     VGTIGLVDDDVVDASNLQRQVVHSTDRIGLSKTESAERTLAGLNPDVRVVRHDERMVA
                     DNVDALIAGYDVIVDGSDNLETRYLLNDAMLRARIPVVHGSVYRWDGQVTTFLPFDGP
                     CYRCLHPEPPPPELAPACDVAGVMGVLPGIIGLLQATEALKVLLGVGRSLSGRLLMVD
                     ALEARFEEVAFARDPGCPACGRG"
     gene            3040..3459
                     /locus_tag="VFV59_10635"
     CDS             3040..3459
                     /locus_tag="VFV59_10635"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEU5326327.1"
                     /translation="MRQGLTLPRDLADALRRHAEAAAPREACGLLGAIPGSAVATTFR
                     PARNVAEVAYLYRMDPGEVVRLVDQMTAAGEGLLAIVHSHTASPATPSPTDRREAAWP
                     DAIHVIVSLDHRVPEPERIRAWQIRDAAAEEVSLDLG"
     gene            complement(3470..4177)
                     /locus_tag="VFV59_10640"
     CDS             complement(3470..4177)
                     /locus_tag="VFV59_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326328.1"
                     /translation="MSPRRALAALVGAAVLLAACAASVSPQPTAEERTPAATASAAAS
                     ASATAAESATQVPGPGWPQAGLPYDGAEILDAMRSSRRPGGVPDALETDPVAQAVAER
                     LWTIDGTAWDTIAASGSCTSATACRLELSGSRSGWLGEDVWVLDIDPSVPTISVADTT
                     LGSVPPGVATDLEGLAASLVDLEGLGLTSVRWTPPPDDERFELSYRDAGEEGGCTVEL
                     ALDAVAGTILDEQRSGC"
     gene            complement(4174..7107)
                     /locus_tag="VFV59_10645"
     CDS             complement(4174..7107)
                     /locus_tag="VFV59_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEU5326329.1"
                     /translation="MRHPGTGPGRRGAVLLALLIGATLTLPLPTPARAGSPLADAAAE
                     LAATYGGAPGDYSLLYERTASVPLSGAAVWAGKFLDRRSGEIRLVYRDLATGRTDGPR
                     LLGQQRAEAVAAVTALEAKGDAELRGAVTGRGPDERVPVGLWLDVDLAAAEQAVIAAH
                     PELTWDGNRPLVADLDTMRRIRAELWQARAAVLAPALDAFSRQVAALGGSIGYASTSA
                     PLAYVDLPAGRVPELASMPEVASLGLERTWRTSMGSAGPAVQANWSGGSEDQGNGVRV
                     AVVEYHNVRNSSDLAGRVAASYSASGSLAYTGSGTFDHPTWVAGAIAGQGSYPGVAPG
                     ALIISASTGGGGASLTRDRQIIAAADWASNPAGGDADVINASIGQDTATGSEEARRYF
                     DAFVDQGGHLAVAAAGNYTTFGHWDILSPGTAYNVLTVGGLDDRNTSDRWDDGIWYTS
                     SNGSNYRDRTDAPWNTHGDYNKPNVSAPAASVVTANGLGASGTSVASPIVAGIAAQLI
                     ARLPSLALRPEAARAIIMAGAVNHGAMPDGSLNADHEGTGSASAMWANRLLNAGDGTY
                     GGYQMGSMMAGQTVTQDVTVTAGQRVKVVVTWNSRAAAGSDDLLADLDLVVTQPDGSR
                     VGSYTIDNNYEWVEFTASGSGSARIEVRQSRFDGGSERYGLAWSKVNLGTPYRVAGPD
                     RYATAATISSWHFAPGVPVAYVATGRNFPDALAVGPVAGRQGGPVLLTDPSSIPDATR
                     NELARLRPQRIVIVGGEGVVSPGVAQQLQAFTGQPVARLAGADRYATAAGVARASFGP
                     GVPAVFVATGRAFPDALTAGPAAAALGGPVLLVDPTSIPQATADALAWLQPRKVYVVG
                     GPGVVSDGVAAALQGYSAEPVARLWGADRFATAASVAQAFFGGPRFAVYLATGRNFPD
                     ALTAVPAASVNAAPLLLLTQTEVPSATANELLRIRPARTYVAGGSGVVTDGVLGGITW
                     LLGKP"
     gene            7227..8363
                     /locus_tag="VFV59_10650"
     CDS             7227..8363
                     /locus_tag="VFV59_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5326330.1"
                     /translation="MTPASSAALRFSPGVHRDRLLRASRAAAERDIDSLLITPSPDYT
                     YLLGYQAPALERLTCLVVPAEGRPALVVPRLEAPLARHELGELVDEVELYAWDETDDP
                     FRLVQRLVGASMRVAVQDQMWARFSLALRAALDPAEMVVAGPAMASLRRIKAPEEVER
                     LRAAASAADSAMRAITGERLSGRTEAEVSARIRGLLLDAGHQSADFAIVASGPNAASA
                     HHVPGDRVIADGDAVVLDIGGTRDGYCSDTTRTAFVGQPAGEFAALYSVLRDAQRTAC
                     DAVRPGVAAADIDAAARNVITDAGYGEAFIHRTGHGIGMETHEEPYLVATNRQPLEEG
                     NAFSVEPGIYVEDTWGARIEDIVVCTADGGERLNTTSTELYLVE"
     gene            complement(8386..9051)
                     /locus_tag="VFV59_10655"
     CDS             complement(8386..9051)
                     /locus_tag="VFV59_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5326331.1"
                     /translation="MPARPPAGDPPPEPDDPWLPLGAASRLVGVGPDTLRRWADTGKV
                     QSYQTPGGHRRFLRSSLEAMINAPRRTRYGMDRLQGSTQTMAGELHRRVQRTGFAGAP
                     WQARLSLEQREEFRRWGQRTFQLVIDYVAATKKAERDLLLHEAERMGALYGAEASAAG
                     LSLAETVEAFLFFRSPVIDAITGHLRRRAAEVSEVTAAFREASAAIDQVLVAVVSSHR
                     ERR"
     gene            complement(9126..10553)
                     /locus_tag="VFV59_10660"
     CDS             complement(9126..10553)
                     /locus_tag="VFV59_10660"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5326332.1"
                     /translation="MTSQIDSDRMTDAALAYLREHDAEHLAQLDDFLRIESVSADPER
                     NGEVRRAAEWIVAQMESLGIERATIHETSAHPIVTGEWMHAGEAAPTVLVYCHYDVQP
                     ADPLDEWVRPPFEPRHEDERVYARGAGDDKGQLFMHLKAVEAWMRTAGRLPVNLRFFF
                     EGDEEVGSEPIEAFIAANPDLLRADVCVVSDGDTFDDDGTPAIGYGLRGIAYWEIKVR
                     GPRQDVHSGQYGGGVENPANVLVRLLASLVDQDGRVTLPGFYDDVRDLSEEERAAYAE
                     LPFDERAWADETGVPEPMSGERGYTLKERLSARPTFDVNGIWGGYQGEGAKTIIPAWA
                     AAKISTRLVPDQDYRTIEQAMKAHLEAIAPPTVRTEVRIIHGGAPAITPLDHPGIAPA
                     RRALTAGFGREPLFQRSGGSVPVVAALDAQLGLKTLMLGFANPTGNFHAPNEWMSLLN
                     LRSGLASLVQLWGELGGMDARDLRA"
     gene            complement(10593..10865)
                     /locus_tag="VFV59_10665"
     CDS             complement(10593..10865)
                     /locus_tag="VFV59_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326333.1"
                     /translation="MLPIVWTLFAVLAVGGFLMIAAYWLDVQDRPDLTFRGRIAWSVG
                     VLLFPLTIPAYAFAGGADWPPFLRAASLVPIVAVALFLGFVFGVFA"
     gene            complement(10878..>11178)
                     /locus_tag="VFV59_10670"
     CDS             complement(10878..>11178)
                     /locus_tag="VFV59_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326334.1"
                     /translation="AFAMASGVPIVPCAVVGTTELWLRKRIEVRFGPPIRPEPNLRGH
                     AGRNALEARLREAIIALLPAGEPRRPRRRPLARLLTDLLNGADDVARRRSELAER"
BASE COUNT         1508 a         4043 c         4112 g         1515 t
ORIGIN      
        1 aagaacgtgg ccgagggcat caacgccagc ctctcgccgg agatgggcgt gctgctgatc
       61 acccactacc agcgcatgct caactacgtg aagccgcagt acgtgcatgt gctgatgaac
      121 ggacggatcg tgatgagcgg cggcgaggag ctgagccacc agctggaggc gaacggctac
      181 gactgggtcc gcgagcaggt cgggctgccc gccgggatcg gtgacgaggc cgaggcgccg
      241 gagcccgtcg gctcccacta ggccacgacc acgagcggag gcggagggcg agcggtgacc
      301 gtcgatcagg cacctgccgg gctcgagggc gccaccctcc gcgccgactt ccccgtcttc
      361 gaccggccgc accggtcggg gcggccgctg gtcttcctgg actccgccgc gtcggcgccg
      421 aagccgcgtg tcgtgatcga ggcgctggcc gacacctacg cgcaccacta cgccaacgtc
      481 catcgtggcc tgtacgagct gagcgaggac gccacggccc gttacgaggc cgcccgccgg
      541 gcggtggcgc gcttcatcgg cgcgccgagc gaacgtgaga tcgtcttcgt gcgcaacgcg
      601 accgaggcca tcaacctggt ggcgtacagc tgggcgcgca acaatctcgg ccctggggac
      661 ctggtcgtct cgacccagat ggagcatcac gccaacctgg tgccgtggca gcaggtcgcg
      721 gccgccaccg gcgcgacgct ggcccacgtg cgcatcaccg acgacggccg cctcgaccgc
      781 gacgacctgc gcgcgcaact ggcccgtggt ccgaggctgc tggccttcgc ccacgtcagc
      841 aatgccctcg gcaccatcaa cgacgtggcg gagatcacgc gcctggccca cgaggccggc
      901 gccctcgtgc tgctggacgc ggcgcagagc gttccgcacc tgccggtctc ggtggcggag
      961 ctggacgtcg acttcgccgt gctctccggc cacaagatgc tcggcccgtc cggcatcggt
     1021 gcgctgtggg cgcgccgcga gctgctggac ggtatgccgc cgttcctgac cggcggcagc
     1081 atgatcaccc gcgtctccct gaccgaggcg gagtggaacg aggtgccctg gaagttcgag
     1141 gccggcaccc cggccatcgc cgaggcggtg ggcctgcacg cggccatcgg ctatctcgag
     1201 gcgctgggca tggagcgcgt tcgtgaccac gagcggcacc tgttcacgcg cgcctgggag
     1261 gccctgggta agctccccgg cgtgcgccga ttcggcccat ccgacccgga tcagcacgcg
     1321 ggcctgatca gcttcgacgt cgcgggcgtg cacccgcacg acgtcgccac catcctggac
     1381 gcggaggggg tggccgtgcg tgccggtcac cactgcgcac agccggtaat ggagcgtttc
     1441 gacgtgccgg cgaccacacg cgcgagcttc aacgtgttca atgacgtgga cgacatcgac
     1501 cgcctgaccg atgccattcg agccgtccag cgcatcttcg gggtcgcctg atggacaacc
     1561 tgtaccgcga cttcatcctg gagcactacc gcaaccccca caaccgcggc tacctgcacc
     1621 cgcacgacct gcacttcgcc gatgcgaacc ccacgtgcgg cgacgagatg tcgatgacgc
     1681 tccggctaga cgcggaccac cagcaggtca ccgacgtcgc gttcgaggga cgcggctgcg
     1741 ccatcagcca ggcatcggcc tcgctgctga ccgatgagct gcgcggccag agcctggacg
     1801 agctgcgcgc catggacccg cgcgacgttc tcgacagcct gggcgtgccg atcggcccgg
     1861 cgcgcttcaa gtgcgcgctc ctgagctaca aggtcctcca gggagcgctt cgcggcgccg
     1921 aggtgcagtg gcccgaggag gccgaggtgc tgggcgtggc gaacgagggc ggctcggcgc
     1981 catgacctgg cgcgaggtcg gcgccgagga ggcggtgtcg ctcgcgcagg ccggcaccac
     2041 ggtgatcgac gtccgcgagg cgaccgagtg gcgcgccggg cacgtggccg gcgccgtcca
     2101 tgtgccgctg ggggaggtgg gcgaccgcgt tcccgccatc gttcccgatc gcgacgcgcc
     2161 gctgctggtg tattgcgccg tcggcgtccg ctccgcccac gccgccgagt ggctgtcgcg
     2221 ggccggctac tcgagggtcg ccagcctgcg ggcatcgctg gacgactggc gccgcgcggg
     2281 cggcggctgg gtcgcggagc cgtcgatgct ggacgagcac gcgcggcggc ggtacgcgcg
     2341 tcagatcccg atccccgagg tcggcgagga ggggcagcgc cgactcctgg actcgcgcgt
     2401 cctgctggtc ggcgccggcg gcctcggttc gcccgtggcg ctgtacctgg cggcggccgg
     2461 cgtggggacc atcggcctcg tcgacgacga cgtggtggac gcgtcgaacc tccagcgcca
     2521 ggtggttcac tcgaccgatc gcatcggcct gtccaagacg gagtccgccg aacggacgct
     2581 ggcggggctc aatcccgacg tgcgcgtggt ccggcacgac gagcgcatgg tcgccgacaa
     2641 cgtcgacgcg ctgatcgcgg ggtacgacgt gatcgttgac ggcagcgaca acctcgagac
     2701 gcgctacctg ctcaacgacg ccatgctccg cgcccgcatc ccggtggtcc acggctcggt
     2761 gtaccgatgg gatggccagg tcacgacgtt cctccccttc gacggtccct gctaccgatg
     2821 cctgcacccg gagccgccgc cgcccgagct ggcgcccgcc tgcgacgtgg ccggcgtcat
     2881 gggcgtcctg cccgggatca tcggtctgct gcaggcaacg gaggcgctga aggtgctcct
     2941 cggcgtcggc cgctcgctgt ccgggcgcct gctgatggtg gacgcgctcg aggcgcggtt
     3001 cgaggaggtc gccttcgccc gcgaccccgg ctgcccggcg tgcggcaggg gctgacgctt
     3061 cctcgcgatc tggcggacgc gctgcggcgc catgccgagg ccgcggcccc gcgcgaggcg
     3121 tgcgggcttc tcggcgccat ccccggttcg gcggtggcga ccacgttccg gcccgcgcgc
     3181 aacgttgccg aggtcgcgta cctctaccgc atggatcccg gcgaggtcgt gcgcctggtc
     3241 gaccagatga cggccgcggg cgaggggctg ctggcgatcg tccactcgca caccgcgtcc
     3301 ccggcgaccc cgtcgcccac cgaccggcgc gaggcggcct ggcccgacgc catccacgtc
     3361 atcgtctcgc tcgaccaccg ggtcccggag ccggagcgca tccgggcgtg gcagatccgc
     3421 gacgccgcgg cggaggaggt ctccctggac ctcggctgag gccgcggggt cagcagccgc
     3481 tgcgctgctc gtcgaggatc gtgcccgcca ccgcgtcgag cgcgagctcg acggtgcagc
     3541 cgccctcctc gccggcatcc cggtagctga gctcgaatcg ctcgtcatcg ggcggcggcg
     3601 tccagcgcac gctggtcagg cccaggccct ccaggtcgac gagtgacgcg gccaggccct
     3661 cgaggtcggt cgcgacgccc ggcgggacgg atccgagggt ggtgtcggcg acgctgatgg
     3721 tcgggaccga gggatcgatg tcgaggaccc acacgtcctc gccgagccac ccgctgcggg
     3781 atcccgacag ctcgagacgg caggccgtcg ccgaggtgca gctgccgctg gccgcgatcg
     3841 tgtcccaggc ggtgccgtcg atggtccaca gccgctcggc cacggcctgg gcgaccgggt
     3901 cggtctcgag ggcgtcggga acgccgcccg gtcgtcggga cgagcgcatg gcatccagga
     3961 tctcggcacc gtcgtacgga agtcccgcct gcggccaccc ggggccgggc acctgggtgg
     4021 ccgattcagc cgcggtcgcc gacgccgatg cggccgcgga tgccgtggcc gcaggagtcc
     4081 gctcctcagc cgtgggctgt ggcgagacgc tcgccgcgca cgccgcgagc agcaccgccg
     4141 ccccgaccag cgcggccagc gcccgccgcg ggctcatggc ttgcccagga gccaggtgat
     4201 cccgcccagg acgccgtcgg tcacgacgcc ggatcctccg gcgacgtagg tccgggccgg
     4261 gcggatccgc agcagctcgt tcgccgtggc ggacgggacc tcggtctggg tcagcaacag
     4321 cagcggcgcc gcgttgaccg atgccgccgg cacggcggtc agcgcatcgg ggaagttgcg
     4381 gccggtcgcg aggtagaccg cgaagcgggg gccgccgaag aacgcctgcg ccaccgatgc
     4441 ggccgtcgcg aagcggtcgg cgccccagag gcgggcaacg ggctccgcgc tgtagccctg
     4501 gagcgcggcc gcgacgccgt cactcacgac gcccgggccg ccgacgacgt agaccttccg
     4561 cggctggagc cacgccagcg cgtccgcggt cgcctgcggg atggaggtcg ggtcgaccag
     4621 cagcacgggc cctcccagcg ccgcggcggc cggaccggcc gtcagggcat cggggaaggc
     4681 gcgaccggtc gccacgaaca ccgcgggcac ccccggcccg aagctggcgc gcgcgacccc
     4741 ggccgcggtc gcgtagcggt cggcacccgc gagccgggcg accggctggc cggtgaaggc
     4801 ctgcagctgc tgcgcgacgc cgggcgacac gacgccctcg ccaccgacga tcacgatccg
     4861 ctgcggccgc agcctggcca gctcgttgcg cgtcgcgtcc gggatgctgg acggatcggt
     4921 cagcagcacc gggccgccct ggcgcccggc gacggggccg accgccagcg catcggggaa
     4981 gttgcggccg gtggcgacgt acgcgaccgg cacgccggga gcgaagtgcc acgagctgat
     5041 cgtggccgcc gtggcatatc ggtccgggcc ggccacgcgg tacggcgtgc cgaggttgac
     5101 cttcgaccag gccagtccat agcgctcgct accgccgtcg aagcgcgact gccgcacctc
     5161 gatccgagcc gatccggagc cggacgcggt gaactcgacc cactcgtagt tgttgtcgat
     5221 ggtgtacgag ccgacgcggc tgccgtcggg ctgggtgacg acgagatcga gatcggccag
     5281 caggtcgtca ctgcctgccg ccgcgcggct gttccaggtc accacgacct tcacgcgctg
     5341 cccggcggtc accgtcacgt cctgggtcac cgtctggccg gccatcatcg atcccatctg
     5401 gtacccgccg taggtgccgt cgcccgcgtt gagcagtcgg ttggcccaca tggcggacgc
     5461 cgagccggtg ccctcgtggt cggcgttgag cgagccatcc ggcatcgcgc cgtggttgac
     5521 cgcgccggcc atgatgatcg cccgcgccgc ctcgggacgg agggccagcg agggcagccg
     5581 cgcgatcagc tgggcggcga tcccggcgac gatcggcgac gccacgctcg tgccgctggc
     5641 gccgaggccg ttggcggtca ccaccgaagc ggctggcgcg gacacgttgg gcttgttgta
     5701 gtcgccgtgg gtgttccacg gcgcatcggt ccgatcccgg tagttgctgc cgttcgagct
     5761 cgtgtaccag atgccgtcgt cccagcggtc cgacgtgttg cggtcatcga ggccgccgac
     5821 ggtgagcacg ttgtaggcgg tcccgggcga gaggatgtcc cagtgcccga aggtcgtgta
     5881 gttcccggcc gccgccaccg ccaggtgccc gccctggtcg acgaaggcgt cgaagtagcg
     5941 ccgcgcctcc tccgagccgg tcgccgtgtc ctggccgatg ctggcgttga tgacgtcggc
     6001 atcgccaccg gccgggttcg aggcccagtc ggccgccgcg atgatctgcc ggtcccgggt
     6061 gagcgatgcg ccgcccccgc cggtcgaggc gctgatgatg agcgcgcccg gcgccacgcc
     6121 cgggtagctg ccctgccccg cgatcgcgcc ggccacccag gtcgggtggt cgaaggtccc
     6181 gctgccggtg tacgccaggc ttccgctggc gctgtacgag gcggcaaccc gtcccgccag
     6241 gtcgctcgag ttgcgcacgt tgtggtactc gaccacggcg acgcgaacgc cgttgccctg
     6301 gtcctcggat ccaccgctcc agttggcctg cacggcgggt ccggcgctcc ccatgctcgt
     6361 gcgccaggtc cgctcgagcc ccagggacgc cacctccggc atcgaggcca gctccggcac
     6421 gcgtcccgcc ggcaggtcca cgtaggccag gggtgcactg gtcgacgcat agccgatgct
     6481 gccgcccagg gccgcgacct gtcgcgagaa ggcatccagg gcgggcgcca ggacggcggc
     6541 ccgagcctgc cacagctcgg cccggatgcg acgcatggtg tcgaggtcgg cgacgagcgg
     6601 ccgattgccg tcccaggtga gctcggggtg agcggcgatg actgcctgct cggccgcggc
     6661 gaggtccacg tcgagccaca ggccgaccgg gacgcgctcg tccggaccgc gtccggtcac
     6721 ggcgccgcgc agctcggcgt cgcccttggc ctcgagcgcc gtcacggcgg cgacggcctc
     6781 ggcgcgctgc tggcccagca gccgcgggcc gtcggttcgc ccggtggcga ggtcgcggta
     6841 caccaggcgg atctccccgc tgcgccggtc gaggaacttg ccggcccata cggcggctcc
     6901 cgagagcggc acgctggccg ttcgctcgta cagcagcgag tagtcgcccg gtgcgccgcc
     6961 gtaggtcgcc gcgagctccg cggctgcgtc cgcgagcgga gagccggcgc gcgccggagt
     7021 ggggagcggc agcgtgaggg tcgcgccgat cagcagggca aggaggacgg cgccgcgccg
     7081 tcccggcccg gtccctggat gtcgcactcc cctcctcctc ccgcgtatga cggcgtatga
     7141 cagcggatga cagtgtgaca cgccgcgctt ccagcctgcg ctcgacccag gccgaccgca
     7201 atgggtcatc ggtgtagcct tctttcatga ctcctgcctc gagcgcggcg ttgcgttttt
     7261 caccgggcgt ccaccgcgac cggctgctgc gcgcgtcgcg cgcggcggcg gagcgcgaca
     7321 tcgactcgct gctgatcacc ccgtcgccgg actacacgta cctcctcggc taccaggcac
     7381 cggccttgga gcggctgacg tgcctggtcg tgccggccga gggccggccg gcgctcgtcg
     7441 tccctcgcct ggaggcgccg ctcgcgcgcc acgagctggg tgagctggtc gatgaggtcg
     7501 agctgtacgc ctgggacgag accgacgacc cgttccggct ggttcagcgc ctggtcggcg
     7561 cctccatgcg cgtcgcggtc caggaccaga tgtgggcgcg gttctcgctg gccctgcggg
     7621 ccgcgctgga cccggccgag atggtcgtgg ccggccccgc catggcgtcc ctgcgacgca
     7681 tcaaggcacc cgaggaggtc gaacgcctgc gcgccgcggc ttcggccgcc gactcggcca
     7741 tgcgcgccat caccggcgag cggctatccg gacggacgga ggccgaggtc agcgcgcgca
     7801 tccgtggtct gctgctggac gcggggcacc agtccgccga cttcgccatc gtggcgtccg
     7861 gcccgaacgc ggcgagcgcg caccacgtcc ccggcgaccg cgtcatcgcg gacggcgacg
     7921 ccgtcgtcct ggacatcggc ggcacacgcg acgggtactg cagcgacacg acgcgcaccg
     7981 ccttcgtggg gcagccggcc ggcgagttcg cggcgctgta ctcggtcctg cgcgatgcgc
     8041 agcggacggc ctgcgacgcg gtgcgtcccg gcgtagcggc cgctgacatc gacgccgccg
     8101 ctcggaacgt catcaccgac gccggctacg gcgaggcgtt catccaccgc accgggcacg
     8161 gcatcggcat ggaaacccac gaggagccgt acctggtggc gaccaaccgg cagccgctgg
     8221 aggagggcaa cgccttcagc gtggagcccg gcatctacgt cgaggacacc tggggcgcgc
     8281 gcatcgagga catcgtggtg tgcaccgccg atggcgggga gcgcctgaac acgacctcga
     8341 ccgagctcta cctggtcgag tagcgcgcgt cggcgcgccg gggcgtcagc gccgctcgcg
     8401 gtgggagctg acgacggcga ccagcacctg gtcgatggcc gcgctcgcct cgcggaaggc
     8461 ggcggtcacc tcgctgacct ccgcggcccg gcggcgcagg tggccggtga tcgcgtcgat
     8521 caccggggag cggaagaaga ggaacgcctc gaccgtctcg gccaggctca gcccggcggc
     8581 cgaggcctcg gcgccgtaca gcgcccccat ccgctcggcc tcgtgcagca ggaggtcgcg
     8641 ctccgccttc ttcgtggcgg cgacgtagtc gatgaccagc tggaacgtcc gctgacccca
     8701 gcggcggaac tcctctcgct gctccaggct gagccgcgcc tgccagggcg caccggcgaa
     8761 cccggtgcgc tggacgcgtc gatgcagctc gcccgccatc gtctgcgtcg aaccctggag
     8821 gcggtccatg ccgtaccggg tgcgccgcgg cgcattgatc atggcctcga ggctggagcg
     8881 caggaagcga cggtggccgc cgggcgtctg gtagctctgc accttgcccg tatcggccca
     8941 gcggcgcagg gtgtcggggc cgacaccgac gaggcgcgat gcggcaccga gcggcagcca
     9001 ggggtcgtcg ggttccggcg gcggatcgcc ggccggcggt cgtgcaggca cgggctggtc
     9061 caaatcagcg gaaatctcgg ccgaaggtag tcgcgcgccc gcgggcgcgc aagtgatgaa
     9121 cggccctagg cgcgcaggtc gcgcgcgtcc atgccgccca gctcacccca gagctgaacg
     9181 agggatgcca gcccgctgcg aaggttcagc aggctcatcc actcgttcgg cgcgtggaag
     9241 ttgccggtcg ggttggcgaa gccgagcatc agcgtcttga ggcccagctg cgcgtccagg
     9301 gcagccacga ccggcaccga tccacccgat cgctggaaca ggggctcgcg gccgaagccg
     9361 gcggtcaggg cccggcgcgc cggcgcgatg ccgggatggt cgagtggcgt gatggccgga
     9421 gcgccgccgt ggatgatgcg gacctccgtt cgcaccgtcg gcggcgcgat cgcctcgagg
     9481 tgcgccttca tggcctgctc gatggtgcgg tagtcctggt cgggcaccag ccgggtcgag
     9541 atcttcgcgg cggcccaggc ggggatgatc gtcttggcac cctcgccctg gtacccgccc
     9601 cagatgccgt tgacgtcgaa cgtcgggcgt gccgagaggc gctccttcag ggtgtagccg
     9661 cgctcgccgc tcatcggctc ggggacgccc gtctcgtcgg cccaggcgcg ctcgtcgaag
     9721 ggcagctcgg cgtaggcggc gcgttcctcc tcggagaggt cgcggacgtc gtcgtagaag
     9781 cccggcagcg tgacgcggcc gtcctggtcg accagcgacg ccagcagccg aaccaggacg
     9841 ttggccgggt tctcgacgcc gccgccgtac tgcccggagt ggacgtcctg ccgcggcccg
     9901 cggaccttga tctcccagta cgcgatgccg cgcaggccgt agccgatggc gggggtcccg
     9961 tcgtcgtcga aggtgtcgcc gtcgctgacc acgcagacgt cggcccgcag caggtcgggg
    10021 ttggcggcga tgaacgcctc gatcggctcg gagccgacct cctcgtcgcc ctcgaagaag
    10081 aaccgcaggt tgactggcag ccggccggcg gtgcgcatcc aggcctcgac cgccttgaga
    10141 tgcatgaaca gctggccctt gtcgtcgccg gcgccgcggg cgtacacccg ctcgtcctcg
    10201 tggcgcggct cgaacggggg acggacccac tcgtccagcg gatcggcggg ctggacgtcg
    10261 tagtggcagt acaccagcac ggtcggtgcc gcctcaccgg cgtgcatcca ctcgccggtc
    10321 acgatcggat gggcgctggt ctcgtggatc gtggcccgct cgatcccgag cgactccatc
    10381 tgcgccacga tccactccgc cgcgcgccgg acctcgccgt tgcgctccgg atcggccgag
    10441 acgctctcga tgcgcaggaa gtcgtcgagc tgcgcgagat gctcggcatc gtgctcgcgg
    10501 aggtaggcga gggccgcatc ggtcatgcgg tcgctgtcga tctggctcgt catggagccg
    10561 atgataggcc gatgcggccg caccggccgc cgtcaggcga agacgccgaa gacgaagccc
    10621 aggaagaggg ccaccgcgac gatgggcacc aggctggccg cacgcaggaa cggtggccag
    10681 tcggccccgc ccgcgaaggc gtaggccggg atggtcagcg ggaagagcag cacgcccacc
    10741 gaccaggcga tgcgcccgcg gaacgtcagg tcgggacggt cctgcacgtc gagccagtac
    10801 gcggcgatca tcaggaagcc gcccacggcg agcacggcga agagggtcca gacgatgggg
    10861 agcatgccgg accgatgcta ccgctcggcg agctcgctgc ggcggcgcgc cacgtcgtcc
    10921 gcgccgttca acaggtcggt cagcagccgc gcgagcggac ggcggcgagg gcggcgcggc
    10981 tcgccggccg gcagcagcgc gatgatggcc tcgcgcaggc gcgcctcgag cgcatttcgg
    11041 cccgcgtgcc cgcggagatt gggttccggc cggatgggcg ggccgaagcg cacctcgatc
    11101 cgcttgcgca accacagctc ggtcgtcccg accacggcgc acggcacgat cggcacgccg
    11161 ctggccatgg cgaaggcg
//
LOCUS       DASTYP010000247        15871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1050390, whole genome shotgun sequence.
ACCESSION   DASTYP010000247 DASTYP010000000
VERSION     DASTYP010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050390"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFV59_10675"
     CDS             <1..273
                     /locus_tag="VFV59_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEU5326335.1"
                     /translation="VVGTHTHVPTADARVLPNGTAYISDIGMTGPRDSVIGFALDTVL
                     PRFTRHLPTRFQVASGPVSFNAVVITADRATGRASSIQQVQRLVEV"
     gene            270..1205
                     /gene="miaA"
                     /locus_tag="VFV59_10680"
     CDS             270..1205
                     /gene="miaA"
                     /locus_tag="VFV59_10680"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU5326336.1"
                     /translation="MSRTDSPLVAIVGPTASGKTDLGLALARHLPVEILVADSRQVYR
                     GMDIGTAKPDAAARKAVPHHLLDLVGPDEPFTVAQWVDAARMLIPDIAARGRLPLVVG
                     GTGLYVAALIEGHDYAAQAWSPDVRRRLTDELEGGGLEPLAERLRVLDPGAAAQTDLR
                     NPRRVLRALERAEAGGGGVRPRVEPYAGRLALIGLDRPREVLYRRIDERAAWLFANGL
                     LDEVRALRDRGYGPDLRPMTGHGYGEALRVLAGEWSLAQAIEVTARRTRQYAKRQLTW
                     FRRDPRIVWLPAGDRPADDPALVARAVDLVRRMVA"
     gene            1232..2554
                     /gene="hflX"
                     /locus_tag="VFV59_10685"
     CDS             1232..2554
                     /gene="hflX"
                     /locus_tag="VFV59_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU5326337.1"
                     /translation="MSERERMIDLTTPEERAFLIGLDDPSDRRWPVQRSLAELAALAE
                     TAGATVVGSAFQRRPQPDPVWYFGKGRAAELVDEKAASDFNVLVVDDELDPNQQRSLE
                     KLLDCKVIDRSALIIDIFARHAKTREGRLQVELAALEYHLPRLTRMWTHLSRTGGGIG
                     TRGPGESQLETDRRLIREKIKKVKAELTEVKRHRATASRQRDRHEVATVALVGYTNAG
                     KSTLLNALANADVYAADMLFATLDPTSRQVTLPTGRQIVVSDTVGFINKLPHDLVDAF
                     RATLEEVMRADLLLEVVDASDPDFVGQQVAVQSVLDELGAGDKPRITVYNKIDLLPTD
                     GDLPPASEQAAFVSAVTGQGLPELLERVGDVLRRQMVAVDAVVPYERGELVARAKHSG
                     DVEEQYEEHGVRVSGHLPQALASELTAAGRNGRSGRSGHAGRRSSVGS"
     gene            2565..3824
                     /locus_tag="VFV59_10690"
     CDS             2565..3824
                     /locus_tag="VFV59_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEU5326338.1"
                     /translation="MPRDTSLRDELLAMADAELEAGSDSELRQRSELDAPRLDAIVAE
                     HGWPGLRLVGADAADAAWLIAYHADADNAQRRGWLPLLAEAVATGDADPRHLATLTDR
                     IAATDRQPQVYGTILMLAADGEVELPVPAVEPARLDERRAEIGLPPVAADAAHLEGGD
                     IIPYRAERGSLPVAQWPMVLEGHVSVEAALEAGVRRVHRVWAVRPGDRRLGRLRALAR
                     ERGVLIEQVERQTIDELASGRTHGGVVALVGPRQERSVEQLFDEVGEGSLVVMLDGIE
                     DPFNYGQAVRALYAAGIDGLVGRRSWETAVSTVTRASAGATELLPAASAASAEDAARV
                     ARSRGLRIACAVADADAVELSEADLTGSLLLYVGGERRGVTRSFVEQADVLVRIGYGR
                     ERAPELGAAASAAIIGFEALRQRRLAT"
     gene            complement(3821..4066)
                     /locus_tag="VFV59_10695"
     CDS             complement(3821..4066)
                     /locus_tag="VFV59_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326339.1"
                     /translation="MAHRLWSIGVAGLGLGSLAMVGGTVVGGDLGPLAAGYGLLVVLS
                     AAWLLGGMVIRERPWRRLAQPLRETTKLESLAGRRVF"
     gene            4264..4665
                     /locus_tag="VFV59_10700"
     CDS             4264..4665
                     /locus_tag="VFV59_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326340.1"
                     /translation="MATPIVYVDRSRITTSDIAALKAATRQLAAFVEAHEPQLLHYAI
                     FVDVQHASMSVVAVHPDSASLAVHLAIGGPEFRKVGAFIELERIDVFGQPSSDVVAKL
                     REKADALGRNAHVHVVTDGAGFSRLGSDPGV"
     gene            complement(4683..6116)
                     /locus_tag="VFV59_10705"
     CDS             complement(4683..6116)
                     /locus_tag="VFV59_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU5326341.1"
                     /translation="MLLHPAPPSSALRAGLLAVILLLAACGAAAAPSDSPPSATPDQA
                     APQPVVIDTDLGADDLIALTLLLREPALDVRAVTVAATGLVHCEAGRQHLSNLFHQLQ
                     VEAVPVGCGRDDPGADGRKFPPEWRTAADAAYGLRMTPVAVGGDRPEAVDVLTRAIED
                     AGAPVTVVALGPWTNLEDLFAASPDLAASIELLHAMAGTVDAPGNVYDEDGAPLEPSL
                     EWNVAADPSAFAAVQEIDVPIRLVSLDATDQVPIDQQLYERLDDDHTAAGANLAFELL
                     TRNAFMLEGGTFLWDELAVLALLDPTLIGWESTPLTVDDAGHLARGGQRSMEVSMEVD
                     PAAVTDALLATLTDGGPRADPFELDGILPVTWDGATCSMDADALAPGLYRLFFTNQSD
                     QAAAAVLATVAPTHEWSELVDFLPKAQTGEEPPSWLAVTIYAGAEASSSASGIGEVGE
                     GTAGPVCLTGDGTDVRAVAGDPIEIAP"
     gene            complement(6246..7337)
                     /locus_tag="VFV59_10710"
     CDS             complement(6246..7337)
                     /locus_tag="VFV59_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5326342.1"
                     /translation="MSDEVGIALVVDDSRVNRLVLTRQLAGLGLEVLEAENGIEALEQ
                     LRATPSAIDVVLLDVMMPELDGYATLEAMKADQAVRHIPVIMVSGVEELDSVVRCIEL
                     GATDYLTKPINARILAARINASLAAKRLHDLQAEYLEQQRQLTATIERQKTELSRFLS
                     PQIAHLISSPEGEHLLAGHRRQITVAFCDLRGFTTFAEQADPEELFSVLGEYHRMMGE
                     VIVEHGGTLEHFAGDGVMIFFNDPVLQEDHVERAVRMATAMRDRFQPMAERWKKRGYE
                     LGFGVGIAVGYATLGRIGFEGRHDYGAIGNVTILASRLSSQAGPNQILLSQRAVGMIE
                     EIVEVEPVGELQIKGLSRPVSASNVLAVR"
     gene            complement(7334..7708)
                     /locus_tag="VFV59_10715"
     CDS             complement(7334..7708)
                     /locus_tag="VFV59_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU5326343.1"
                     /translation="MTDADSPVLDASVLDELSASVGGDRAFVTDLIDAYLTDGAQQVE
                     QVEAAVAAGDPEALVRPAHTLKSSSATVGATRLAAMARELEFAGREGRVDDAQPSHAA
                     ALRAEWDAATSALRDWVSRGDA"
     gene            complement(7722..14255)
                     /locus_tag="VFV59_10720"
     CDS             complement(7722..14255)
                     /locus_tag="VFV59_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5326344.1"
                     /translation="MGPPHPRAVGDAPHRVRAQYARATDACPAPGAHRRAVPIPEPRG
                     SEPPPSVDGQGRQVLGAARRRITELEERAAALERSERIQAALYRIAETASAARDMPAF
                     YEEIHRIVGELMYADNLFIALYDERRRMMNYPFYRDEVDLDVPDPNVWEPLGEGQAAG
                     FTGWVLRTGRPVLLSLGELEAMVERGEVTMLGEPSADWMAAPLQADGHTIGVVVVQSY
                     DEERRHTPEDLELLTFVAQHIATALTRARALEETRQRNAELAVVNEIGTALARQLDFN
                     AIIDLVGERVLSIFGVRGGGIALYDEETRLLSFPFNVDNGERLAMEPVEVGTGLTGKV
                     ISSRQPIRIGSMADARDRGAIFIGDGEQNESWLGVPILAGDRVLGVISIDRMEPNGFS
                     ESDERLLSTLASSMGVALENARLFDETRRLLTETEQRNAELAVVNEIGSALAQQLEFD
                     AVIQLVGERIRGIFKADSIFIGIVDEEAKTVAFPYSIEFGAPTPTDSINIGEGLTSRV
                     LTTGRPLRLSASAEGQELGAVVQGSASESWLGVPIMAGERAIGILGLESAVPNAFSDA
                     DERLLGTLASSMGVALQNARLFDETKRLLAETEQRNGELAVVNEIGSALAQQLDFNAI
                     IELVGNRLSTMFSAVDMYIGLYDEAANEISFPFELEHGRRVHGPPIEFGLGLSSIVLR
                     ERRPLRFGTSEAQTAAGGLIGTYEEESTGRVSDSWIGIPIMAGHRAIGIIAFGAEERD
                     AYSESDERVLSTVASSMGVALENARLFDETKRLLADADERAAELAVVNSVQQGLAANL
                     DMQAMYELVGEKIHEIFDAQVVDIGIIDEAEGVVRYPYSIERGVRFPDEPSPLAASKL
                     TQAMLAAGGPLIVNDVESWEREHGVLPVLQGEPSKSVLLAPLVAGGATRGRISLQNLD
                     RTDAFSEQDVRLLTTLAASLSVALENARLFDETRRLLTEADARAAELAVVNSVQQGLA
                     QNLDMQAMYDLIGDKIQEIFDAQVVDIGIVDHAQGVIHFPYTIERGVRFPDEAMPADA
                     GARGLVLARREPIVVNRDLRGWAEREGLEWHIPQGEAPQSALWVPLIVGDEPRGVISL
                     QNLDREDAFGDQDVRLLTTLAASLSVALENARLFDETRRLLAETDARAAELAIINSVQ
                     DKLAGELDIQSMYELVGERASDVFDTQVVDIAIFDRNTGMMSFPFSVERGQRFDIEPR
                     EVMGFRKHVVETRAPLLITHDLRARGQELGQPDHIVGEPARSAIFTPLIVEDEVLGVM
                     SLQNLDREYAFDEGDVSLLSTIAASLSVALRTGRLIDETRRRVSELATINDVGEAISA
                     QLEVEPLLALVGEKARDAFDADIAYVALVDEERGVIDFPYYVEGGAHEPQEPLQIGEG
                     LTSRVLDLKRPLLLNRDEDWARLGQRGRGTLARSWLGVPIMTGDRAIGVISIQSTRQE
                     GRFGESDERLLATIAANVGVAVQNARLYDEARRRAEEMAALADVGREMSATLELSVVL
                     ELIADRARTLLGSDTSAVFLPTEDTETLRATVALGEIAEQIRNDAITRGEGVIGDIAL
                     TGRPEVVNSTSSDSRSVTIPGTEDIDDRLMAAPLTVRGDVAGILAVWRGADSDPYTAA
                     DLEFLVGLSQQAAIAIQNARLFADAQESRQAAEQANEAKSSFLAAMSHEIRTPLNAVI
                     GMSGLLLDTELDTEQREFAETIRTSGDALLTIINDVLDFSKIEAGRVELEARPFVLRE
                     AIEASLDILAPSAAKKGLELVYAIEEELPANLVGDAGRLRQMLLNLLSNAVKFTERGE
                     VVVTVGGARMPAAGRKGSERWEVRIDVRDTGIGIPPDAMGKLFQSFSQVDASIARRYG
                     GTGLGLAISRRLAELMDGSLTAESSGVAGEGSVFHLAVRMPVAAPDAVASSRPMRIEA
                     DLSGRRVLIVDDNATNRRILVAQTARWGMVPRETGSPLEALGWLTAGERFDIALFDLL
                     MPDLDGLELSERIRALPNGDGRPPLPIVILSSIGLRERDDAPISAWLAKPVKPSALHD
                     TIATVLLGEAVARPAARQPAAPAGGRLGDRHPLRILLAEDNPVNQKLALRLLAQLAYE
                     PDVAGDGLQAIAALEEGEFDVVLMDVQMPELDGLEATRRIRGRWPGRPVWIVAMTANA
                     MAGDREACLAAGMNDYISKPIRPTELAAALERAPAGVAEPEAAEQ"
     gene            14254..14580
                     /locus_tag="VFV59_10725"
     CDS             14254..14580
                     /locus_tag="VFV59_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5326345.1"
                     /translation="MNDSYTYLDDLAAEASIPKRGIHSQTLSDADGVELVLFAMAAAE
                     RLSEHTSARSAIVHVLAGSGDLVVAGDAYRLSPGSWMRMRARTPHAITAGDEGLVFAL
                     YLLASS"
     gene            complement(14590..14985)
                     /locus_tag="VFV59_10730"
     CDS             complement(14590..14985)
                     /locus_tag="VFV59_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5326346.1"
                     /translation="MHPRRAIMALTLAILLGGCGGTAVAPGDAAVRQLPAAEAVAEVP
                     SRVLIDVRTPDETADGMIAGALNIDFEAADFRERISQLDRNGKYLLYCRTGNRSAQAA
                     AVMAELGFTDVIDAGGFSDLVAAGAPVEP"
     gene            complement(14994..15371)
                     /locus_tag="VFV59_10735"
     CDS             complement(14994..15371)
                     /locus_tag="VFV59_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326347.1"
                     /translation="MIGARAGVLGALLALAVALAYQAVVRLVDASGARDQPAVILILL
                     LSAFLGVLTAVATRMLRLPGATAPTVLVLGWTLVPVVLAAIPSQATQLFAGDAALSAG
                     QALALATATVAAAVTLGVPAERR"
     gene            complement(15368..>15871)
                     /locus_tag="VFV59_10740"
     CDS             complement(15368..>15871)
                     /locus_tag="VFV59_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEU5326348.1"
                     /translation="RLAMALETFAGAGRRMELIADTPRVTIYDDYGHHPTEVRAALSA
                     ARQKVGPTRRLWAVFEPHMYSRTALLMDEFSTAFTDANEVIIADIFASRDTPEAMAAT
                     SAADLADAIERASGIPALATGDVDATTAYVAEHLADGDAVLVMGAGKSYRIARGLRDR
                     LAGESGA"
BASE COUNT         2259 a         5548 c         5714 g         2350 t
ORIGIN      
        1 gtcgtcggca cgcacacgca tgtcccgacg gccgatgcgc gcgtcctgcc caacggcacc
       61 gcctacatca gcgacatcgg catgaccggg ccgcgcgact cggtcatcgg gttcgcactg
      121 gacaccgtgc tgccgcgctt cacgcggcac ctgccgaccc gcttccaggt cgcgtccggc
      181 ccggtctcgt tcaacgccgt cgtgatcacc gcggataggg ccaccggccg cgccagctcg
      241 atccagcagg tccagcgcct cgtcgaggtg tgagccggac ggattccccg ctcgtcgcga
      301 tcgtcgggcc gacggcatcc ggcaagaccg atctcgggct ggcgctggcg cgccacctcc
      361 cggtcgagat cctggtggcc gactcgcggc aggtgtaccg cggcatggac atcggtaccg
      421 ccaagcctga cgccgccgcg cgcaaggccg tgccgcacca cctgctggat ctggtcgggc
      481 cggacgagcc gttcacggtc gcccagtggg tggacgccgc gcggatgctg attcccgaca
      541 tcgcggcccg cgggcggctg ccgctcgtcg tgggcggcac cggcctctac gtcgcggcgc
      601 tgatcgaggg ccacgactac gccgcgcagg cgtggtcgcc cgacgtgcgg cgacgcctga
      661 ccgatgagct cgagggaggc ggcctcgagc cgctcgccga gcgcctccgc gtgctcgacc
      721 cgggcgccgc cgcgcagacc gacctccgca acccgaggcg ggtgctgcgc gccctggaac
      781 gcgccgaagc cggtggtggg ggagtgcgcc ctcgcgtcga gccgtacgcc ggacggctgg
      841 ccctcatcgg cctggatcgt ccgcgtgagg tgctctaccg gcggatcgac gagcgggcgg
      901 cgtggctgtt cgccaacgga ctgctcgacg aggtgcgtgc gctgcgcgac cgcgggtacg
      961 gaccggacct gcggccgatg accgggcacg gatacggtga ggcgctgcgc gtcctggcgg
     1021 gggagtggtc gctcgcgcag gccatcgagg taaccgcgcg ccgcacccgg cagtacgcca
     1081 agcggcagct cacctggttc cggcgtgacc cgcgcatcgt gtggctgccg gctggcgacc
     1141 gtcccgccga cgacccggcg ctggtggcgc gtgcggtcga cctggtgcgg cgaatggtgg
     1201 cctgaggcgc atcggctacc atgtcgaaca gatgtccgaa cgcgaacgca tgatcgatct
     1261 caccacgccc gaggagcggg cgttcctcat cggcctcgac gatccgagcg accgacgctg
     1321 gcccgtgcag cgctccctgg cggagctcgc agccctggcg gagacggcgg gcgccaccgt
     1381 ggtcggcagt gccttccagc ggcggcccca accggacccg gtctggtact tcggcaaggg
     1441 gcgcgcggcg gagctggtcg acgagaaggc ggccagcgat ttcaacgtgc tcgtcgtcga
     1501 cgacgagctg gatccgaacc agcagcggag cctcgagaag ctgctggact gcaaggtgat
     1561 cgatcgttcc gcgctgatca tcgacatctt cgcccggcat gcgaagactc gggagggccg
     1621 gctccaggtg gagctggccg cgctggagta ccacctgccg cggctgaccc gcatgtggac
     1681 ccacctgtcg cgcaccggcg gcgggatcgg gacccggggt cccggcgaga gccagctgga
     1741 gaccgaccgc cgcctcatcc gcgagaagat caagaaggtg aaggcggagc tgaccgaggt
     1801 gaaacgccac cgggccaccg cctctcgcca gcgcgatcgt cacgaggtcg caacggtggc
     1861 cctggtcggc tacaccaacg ccggtaagag cacgctgctc aatgccctcg cgaacgccga
     1921 tgtgtacgcc gccgacatgc tgttcgccac gctcgacccg accagccggc aggtgacgct
     1981 gccgaccggc cgccagatcg tggtcagcga cacggtcggg ttcatcaaca agctgccgca
     2041 cgacctggtc gacgccttcc gcgccacgct ggaggaggtc atgcgcgccg acctgctgct
     2101 cgaggtggtc gatgcctccg atcccgactt cgtgggccag caggtcgccg tccagtcggt
     2161 gctcgacgag ctcggcgccg gagacaagcc ccggatcacg gtctacaaca agatcgacct
     2221 gctcccgacg gacggtgacc ttcctccggc cagcgagcag gcggcgttcg tgagtgccgt
     2281 gacggggcag gggctgccgg agctgctcga gcgggtgggc gacgtcctgc ggcggcagat
     2341 ggtcgccgtg gatgccgtgg tgccgtacga gcgcggcgag ctggttgcgc gcgccaagca
     2401 ctccggcgac gtcgaggagc agtacgagga gcacggggtg cgggtcagcg gccacctgcc
     2461 gcaggcgctc gcctctgagc tcaccgccgc cggacgcaac ggacgcagcg gacgcagcgg
     2521 acacgccggc cggcgctcgt ccgtcggttc ctgaggccgc ctccgtgcca cgcgacacgt
     2581 cgctgcgcga cgagctgctg gccatggccg acgccgagct ggaggccggg agcgacagcg
     2641 agctgcgcca gcgcagcgag ctggacgccc cacgcctcga cgcgatcgtc gccgagcatg
     2701 gttggcccgg cctgcgcctg gtcggagccg atgcggccga tgccgcctgg ctgatcgcct
     2761 accacgcgga tgccgacaat gcgcaacgcc ggggctggct gcccctgctg gccgaggcgg
     2821 tcgccaccgg cgacgccgat ccgcgccacc tcgccacgct gaccgaccgc atcgcagcga
     2881 ccgatcgcca gccgcaggtg tacggcacga tcctcatgct ggccgccgac ggcgaggtcg
     2941 agctgcccgt tccggcggtg gaaccggccc gcctggacga gcgccgcgcc gagatcgggc
     3001 tgccgccggt ggcggcggat gcagcgcatc tcgagggcgg cgacatcatc ccgtaccgtg
     3061 ccgaacgcgg cagcctgccc gtggcgcagt ggccgatggt gctcgagggc cacgtctcag
     3121 tcgaggcggc gctggaggcc ggcgttcgtc gtgtccaccg ggtctgggcc gttcgtccgg
     3181 gcgatcggcg cctcgggcga ttgcgggcgc tggcccgcga gcggggcgtg ctcatcgagc
     3241 aggtcgaacg ccagaccatc gacgagctgg catcgggacg cacacacggc ggcgtcgtgg
     3301 cgctggtcgg gccgcgccag gagcgcagcg tcgaacagct gttcgatgag gtgggggagg
     3361 ggtcgctggt ggtgatgctc gacggcatcg aggacccgtt caactacggc caggccgtgc
     3421 gggcgctgta cgccgccggg atcgacggac tggtcggccg tcggagctgg gagaccgcgg
     3481 tcagcacggt gacgcgtgcc tcggcgggcg cgacggagct gctgccggcc gcgtcggcgg
     3541 cgtccgccga ggatgcggcg agggtggcgc ggtcacgcgg cctgcgcatc gcctgcgcgg
     3601 tggcggacgc ggacgccgtg gagttgtcgg aggccgatct caccggcagc ctgctgctgt
     3661 acgtgggcgg agaacgccgt ggggtcacgc gttcgttcgt cgagcaggcc gacgtgctgg
     3721 tgcggatcgg ctacggccgg gagcgtgcgc cggagctggg agcggcggct tccgcggcga
     3781 tcatcggctt cgaggcgctg cgacagcgcc ggctggcgac ctagaagacg cggcggccgg
     3841 cgaggctctc gagcttcgtc gtttcccgga gcggctgcgc caggcgtcgc caggggcgct
     3901 cgcggatcac cattccgccc agcagccagg cggccgagag gacgacgagc aggccgtagc
     3961 ctgcggcgag gggcccgagg tcgccgccga cgacggtgcc gccgaccatg gcgaggctgc
     4021 cgagcccgag ccctgcgacg ccgatcgacc acagccggtg tgccatgtgt gcagcgtacg
     4081 ggccgcatgc cgagaacccg ataggaccat cggggcaacc gggcaggact ctcgccgtcg
     4141 gctactcagt ccgctgagcg attactactt ccgcaaatgt atgttatgta acgtatgagg
     4201 tgaacgtggc tcagtgctgt cctccggggg cctgcggttg catgcgcctg caaccatgag
     4261 cgcatggcca cacccatcgt gtacgtcgac cgatcgagga tcaccaccag cgacattgcc
     4321 gcgctgaagg ccgccacgcg ccagctcgcg gcgttcgtcg aggcgcatga gccccagctg
     4381 ctgcactacg ccatcttcgt cgacgtgcag cacgccagca tgtccgtggt cgccgtgcac
     4441 cccgattcgg cgtcgctggc ggtgcacctg gcgatcggcg ggccggagtt ccgcaaggtc
     4501 ggtgcgttca tcgagctgga gcggatcgac gtcttcggtc agccatcgag cgacgtcgtc
     4561 gcgaaactgc gggagaaggc cgacgcgctc ggccgaaatg ctcacgtgca cgtggtgacg
     4621 gacggcgcgg gattcagtcg gctcggaagc gatccgggcg tctgacacgc cgcagcctgg
     4681 gcctacggag cgatctcgat cggatctccg gcgacggcac gcacgtcggt cccatcgccg
     4741 gtcaggcaaa ccggtccggc cgtgccctcc cccacctcgc cgatgccgga ggccgacgag
     4801 gaggcctccg cgccggcgta gatggtgacg gccagccatg acggcggctc ctcgccggtc
     4861 tgggccttcg gcaggaagtc cacgagctcc gaccactcgt gcgttggcgc gacggtggcc
     4921 agcacggccg cggccgcctg gtcggactgg ttggtgaaga agaggcggta gagcccgggc
     4981 gccagcgcat cggcgtccat gctgcacgtc gccccatccc atgtgacggg caggatgccg
     5041 tcgagctcga acggatcggc gcgtggtccg ccgtcggtca gcgtcgcgag gagcgcatcg
     5101 gtcacggcag ccggatcgac ctccatgctg acctccatcg agcgctggcc tccgcgcgcc
     5161 aggtggcccg cgtcgtcgac ggtgagcggt gtcgattccc agccgatcag cgtcgggtcg
     5221 aggagcgcga gcacggccag ctcgtcccac aggaacgtcc cgccctcgag catgaaggcg
     5281 ttgcgggtga gcagctcgaa ggccaggttc gcgccggccg cggtgtgatc gtcatccagg
     5341 cgctcgtaga gctgctggtc gatcgggacc tgatcggtcg cgtcgagcga gacgaggcgg
     5401 atcggcacgt cgatctcctg gacggcggcg aacgccgatg gatcggcggc gacgttccac
     5461 tcgagcgacg gctccagcgg cgcaccgtcc tcgtcgtata cgttgcccgg cgcgtccacg
     5521 gtgcccgcca tggcgtggag cagctcgatc gacgctgcca gatcgggcga ggcggcgaag
     5581 agatcctcga ggttggtcca tggcccgagg gccacgacgg tgaccggcgc gccggcgtcc
     5641 tcgatcgccc gggtgagcac gtcgacggcc tcaggacggt cgccgccgac cgcgacgggc
     5701 gtcatccgca gtccgtaggc cgcatccgcc gccgtgcgcc actccggcgg gaatttacga
     5761 ccatcggcgc ctggatcgtc acggccgcat cccaccggca cggcctccac ctggagctgg
     5821 tggaagaggt tgctcaggtg ctgccgtccc gcctcacagt gcacgagccc ggtggcggca
     5881 acggtgacgg cccgaacgtc gagtgcgggc tcgcgcagca ggagggtcag cgcgatgagg
     5941 tcgtccgccc cgaggtccgt gtcgatcacc accggctgag gagccgcttg gtcgggagtg
     6001 gctgacgggg gcgagtcgga gggagcggcg gcggcgccgc aggcggcgag caggaggatg
     6061 acggccagga ggccggcgcg cagcgccgac gatggcgggg cgggatggag cagcatgggt
     6121 ggctcggaac ctcggtggtg ggcgcgcggg tggcgccgca ggatggtatc gggcgcgcgg
     6181 agcatgggat cagggccgtc cggcccgggc ctgatggtcg gatgggtatg gggccggatc
     6241 ccgcctcagc gcacggccag gacgttactg gcgctcaccg ggcggctcag ccctttgatc
     6301 tggagctcac ccaccggctc gacctcgacg atctcctcga tcatcccgac ggcgcgctgg
     6361 ctcagcagga tctggttcgg gcctgcctgg ctgctcagcc gcgaggcgag gatggtgacg
     6421 ttgccgatgg caccgtagtc atgccgcccc tcgaagccga tgcgcccgag ggtcgcgtag
     6481 ccgaccgcga tccccacgcc gaagccgagc tcgtagccgc gcttcttcca acgctcggcc
     6541 atcggctgga accgatcgcg catggcggtc gccatgcgga ctgcccgctc gacgtggtcc
     6601 tcctgcagga ccgggtcgtt gaagaagatc atcacgccgt cgcccgcgaa gtgctcgagc
     6661 gttccgccgt gctcgacgat gacctccccc atcatccggt ggtactcgcc gaggacgctg
     6721 aacagctcct cggggtcggc ctgctccgcg aacgtcgtga agccccgaag gtcgcagaag
     6781 gcgacggtga tctggcgccg atgtcccgcc agcaggtgct ccccttccgg tgacgagatg
     6841 aggtgcgcga tctgcggcga gaggaatcgg ctcagctcgg tcttctggcg ctcgatggtc
     6901 gcggtcagct ggcgctgctg ctcgaggtac tcggcctgca ggtcgtgcag ccgcttcgcc
     6961 gccagagatg cgttgatgcg agcggccagg atgcgcgcgt tgatgggctt ggtcagataa
     7021 tccgtcgcgc cgagctcgat gcagcgcacc acgctgtcga gctcctccac gcccgagacc
     7081 atgatcaccg ggatgtgccg gacggcctgg tcggccttca tggcctcaag cgtggcgtag
     7141 ccgtccagct cgggcatcat gacgtccagc agcacgacgt cgatggcgga gggcgtggcg
     7201 cgcagctgct ccagcgcctc gatcccgttc tctgcctcca ggacctcgag gccaaggccg
     7261 gccagctgcc gcgtcaagac gagccggttg acccggctgt cgtcgacgac cagcgcgatg
     7321 ccgacctcgt cgctcatgcg tcgcctcggg agacccagtc gcggagcgcc gatgtcgccg
     7381 cgtcccactc ggcgcgcagt gccgccgcgt gcgaaggctg ggcgtcgtcg acgcgtccct
     7441 cacggccggc gaactcgagc tcgcgtgcca tcgccgcgag gcgtgtggcc cccaccgtgg
     7501 cgctgctgga cttcagggtg tgcgcgggcc tgacgagggc ttcgggatcg cctgcggcga
     7561 cggcggcctc cacctgctcc acctgctgcg cgccatccgt caggtaggcg tcgatcaggt
     7621 cggtcacgaa ggcgcggtcg cctcccaccg acgcactcag ctcgtcgagg acgcttgcgt
     7681 cgagcaccgg gctgtcggca tcagtcacgt gcgggctcct ttcactgctc ggcagcttcc
     7741 ggctctgcga cgccggccgg ggcgcgctcc agcgcggcgg ccagctccgt cgggcggatc
     7801 ggcttgctga tgtagtcgtt catcccggcc gcgagacacg cctcccggtc gccggccatg
     7861 gcgttggcgg tcatcgcgac gatccacacc ggccgtcccg gccaccggcc ccggatccgc
     7921 cgcgtggcct cgaggccgtc gagctccggc atctggacgt ccatcagcac gacgtcgaac
     7981 tctccctcct ccagcgcggc gatcgcctgc aggccgtcgc cggcgacatc gggctcgtag
     8041 gccagctgcg ccagcagccg cagggcgagc ttctggttca ccgggttgtc ctccgccagc
     8101 aggatgcgca gcgggtggcg atcccccagc cggccgccgg ccggcgccgc gggctgacgt
     8161 gctgccggtc gcgcgaccgc ctcgcccagg aggaccgtgg cgatcgtgtc gtggagcgcc
     8221 gacggcttca ccggcttcgc cagccaggcg gagatcgggg cgtcgtcgcg ctcgcgcagg
     8281 ccgatggagg agaggatgac gatcggcagc ggcggccggc cgtcgccgtt cggcagcgcg
     8341 cggatgcgct cagagagctc caggccgtcg agatccggca tcagcaggtc gaacagggcg
     8401 atgtcgaacc gctccccggc ggtgagccag cccagcgcct cgagcgggga gccggtctcg
     8461 cgcggaacca tcccccaccg cgccgtctgg gccaccagga tgcgccggtt ggtggcgttg
     8521 tcgtcgacga tgagcacgcg gcgaccgctg aggtcggcct cgatgcgcat cggccggctg
     8581 gaggccacgg catccggcgc ggccaccggc atgcggaccg cgaggtggaa gacgctcccc
     8641 tcccccgcca cgccgctgct ctcggcggtg agtgagccgt ccatgagctc ggcaaggcgg
     8701 cggctgatgg cgagcccgag cccggtgccg ccatagcgac gcgcgatcga ggcgtcgacc
     8761 tggctgaagg actggaacag cttgcccatc gcgtccgggg gaatgccgat accggtgtcg
     8821 cgcacgtcga tgcgcacctc ccagcgctcg ctcccctttc gtccggccgc cggcattcgg
     8881 gctccgccga cggtgaccac gacctcgccg cgctcggtga acttcacggc gttggacagc
     8941 aggttcagca gcatctggcg caggcggccg gcgtcgccca ccaggtttgc cggcagctcc
     9001 tcctcgatgg cgtagacaag ctccaggccc ttcttggccg cggagggcgc caggatgtcg
     9061 agcgaggcct cgatcgcctc gcgcaggacg aacggtctcg cctcgagctc cacccgcccg
     9121 gcctcgatct tcgagaagtc cagaacgtcg ttgatgatcg tcagcagggc atcgcccgag
     9181 gtgcggatgg tttccgcgaa ctcgcgctgc tcggtgtcga gctccgtgtc gagcaggagg
     9241 ccgctcatcc cgatgacggc gttgagcggc gtccgaatct cgtgactcat ggcggcgagg
     9301 aagctgctct tcgcctcgtt ggcctgctcg gccgcctgcc gggactcctg cgcgtcggcg
     9361 aagaggcgcg cgttctggat ggcgatggcg gcctgttgcg acagcccgac gaggaactcg
     9421 aggtcggcgg cggtgtatgg gtcgctgtcg gcgccgcgcc agacggcgag gatgccggcc
     9481 acatcgccgc gcaccgtgag cggcgcggcc atgagccggt cgtcgatgtc ctcggtcccg
     9541 ggaatcgtca ccgaacgcga atccgatgat gtgctgttga cgacctcggg gcgaccggtc
     9601 agggcaatgt ccccgatcac accctccccg cgggtgatgg cgtcgttgcg aatctgctcg
     9661 gcgatctccc caagggccac ggtcgcacgc aaggtctcgg tgtcctcggt cggcaagaag
     9721 acagcgctcg tatcggatcc caggagcgtt cgtgcgcgat cggcgatcag ctcgagcacg
     9781 acggacagct cgagcgtggc ggacatctcg cggccgacgt cggccagggc ggccatctcc
     9841 tcggcgcggc ggcgcgcctc gtcgtagagg cgggcgttct gcacggcgac gccgacgttc
     9901 gcggcgatgg tggccagcag gcgctcatcc gactcgccga agcggccctc ctgcctggtg
     9961 ctctggatgc tgatgacacc gatggcgcga tcgccggtca tgatcggcac accgagccac
    10021 gaccgcgcca gggtgccgcg gccccgctgg cccaggcggg cccagtcctc gtcgcggttg
    10081 agcagcagcg ggcgcttgag gtccagcacc cgggaggtga gcccttcgcc gatctggagc
    10141 ggctcctgcg gctcgtgcgc accgccctcg acgtagtacg ggaagtcgat gacgccccgc
    10201 tcttcgtcca ccagagcgac gtacgcgatg tcggcgtcga aggcgtcccg cgccttttcg
    10261 ccgaccaggg cgagcagggg ctcgacctcg agctgggcgc tgatggcttc gccgacgtcg
    10321 ttgatggtcg ccagctccga gacgcggcgt cgcgtctcgt cgatgaggcg gccggtgcgc
    10381 agcgcgacgc tcaggctggc cgcgatggtg ctcagcagcg agacgtcgcc ctcgtcgaac
    10441 gcgtactcgc ggtcgaggtt ctggagcgac atcacgccga gcacctcgtc ctcgacgatg
    10501 agcggcgtga agatcgcgga acgcgccggc tcgcccacga tgtggtcggg ctgaccgagc
    10561 tcctgtcccc gggcgcgcag atcgtgcgtg atcaggagtg gcgcgcgcgt ctcgaccacg
    10621 tgcttgcgga agcccatgac ctcgcgcggc tcgatgtcga atcgctggcc gcgctccacc
    10681 gagaacggga acgacatcat cccggtgttg cggtcgaaga tcgcgatgtc gacgacctgc
    10741 gtgtcgaaca cgtcgctcgc gcgctcgccg accagctcgt acatcgactg gatgtcgagc
    10801 tcgccggcca gcttgtcctg gacggagttg atgatcgcca gctcggcggc gcgcgcatcg
    10861 gtctccgcaa ggagacgccg ggtctcgtcg aacagcctcg cgttctcgag cgccacgctc
    10921 aggctggcgg ccagcgtggt cagcagccgc acgtcctggt ccccgaacgc gtcctcgcga
    10981 tccaggttct ggagggagat cacgccgcgc ggctcgtcac cgacgatcag cggcacccac
    11041 agcgcggact gcggtgcctc gccctgcgga atgtgccact cgaggccttc gcgctcggcc
    11101 cacccgcgca ggtcgcggtt gaccacgatg ggctctcgcc tggcgagcac cagcccgcgc
    11161 gcaccggcgt cggccggcat cgcttcgtcg gggaaccgca cgccccgctc gatggtgtag
    11221 gggaagtgga tcaccccctg cgcgtggtcg acgatgccga tgtcgaccac ctgggcgtcg
    11281 aagatctcct ggatcttgtc gccgatgagg tcgtacatgg cctgcatgtc caggttctgc
    11341 gcgaggccct gctggacgct gttgacgacc gcgagctccg ccgcacgcgc atcggcctcg
    11401 gtcagcaggc gccgggtctc gtcgaagagg cgcgcgttct cgagggccac gctcaggctg
    11461 gcggccagcg tcgtgagcag gcggacgtcc tgctcgctga acgcgtcggt ccgatccagg
    11521 ttctggagcg agatgcggcc ccgggtggcg ccgccggcga ccaggggcgc caacaggacc
    11581 gacttcgacg gctccccctg cagcacgggc aggacgccgt gctctcgctc ccaggactcg
    11641 acgtcgttga cgatcagcgg cccgcccgcc gcgagcatcg cctgcgtgag tttggacgcc
    11701 gccagcgggc tgggctcgtc ggggaaccgg acgccgcgct cgatggagta cgggtagcgg
    11761 accacgccct cggcctcgtc gatgatgccg atgtcgacga cctgggcgtc gaagatctcg
    11821 tggatcttct cccccaccag ttcgtacatg gcttgcatgt cgaggttcgc ggccaggccc
    11881 tgctggacgc tgttgacgac ggccagctcc gcggcgcgct cgtccgcatc ggccaggagg
    11941 cgcttggtct cgtcgaacag tcgggcgttc tccagggcca cgcccatgct cgaggcgacg
    12001 gtgctgagca cgcgttcatc gctctcgctg tacgcgtcgc gctcttctgc gccaaaggcg
    12061 atgatgccga tggcgcggtg gccggccatg atcgggatgc cgatccagga gtccgagacg
    12121 cgcccggtcg attcctcctc gtaggtgccg atgagcccgc cggccgcggt ctgggcctcg
    12181 gacgtgccga accggagggg gcgtcgctcc cgcaggacga tcgaggacag gcccagcccg
    12241 aactcgatgg gcgggccgtg cacccggcgg ccgtgctcga gctcgaaggg gaagctgatc
    12301 tcgttggcgg cctcgtcgta caggccgatg tacatgtcga ccgccgagaa catggtcgac
    12361 agccggttcc cgaccagctc gatgatggcg ttgaagtcga gctgctgggc gagcgcggag
    12421 ccgatctcgt tgacgaccgc cagctcgccg ttgcgctgtt ccgtctccgc gaggaggcgc
    12481 ttcgtctcgt cgaagagccg cgcgttctgg agcgcgacgc ccatgctgga ggccagcgtg
    12541 cccaacagcc gctcgtcggc atcggagaac gcgttgggaa cggcgctctc gaggccgaga
    12601 atgccgatcg cccgttcgcc ggccatgatc ggcacgccga gccagctctc ggacgccgat
    12661 ccctgcacga ccgccccgag ctcctggccc tccgccgacg cactcaggcg caggggtcgt
    12721 ccggtcgtca gcacgcgcga ggtcagtccc tcgccgatgt tgatgctgtc ggtcggggtc
    12781 ggcgcgccga actcgatgga atacgggaag gcgacggtct tcgcttcctc gtcgacgatg
    12841 ccgatgaaga tcgagtcggc cttgaagatg ccgcggatcc gctcccccac cagctggatc
    12901 accgcgtcga actcgagctg ctgcgcgagc gcggatccga tctcgttcac cacggccagc
    12961 tcggcgttgc gctgttccgt ctcggtcagc agccgcctgg tctcgtcgaa cagccgcgca
    13021 ttctcgaggg ccacgcccat ggacgaagca agggtcgaga ggagacgctc gtcggactcg
    13081 ctgaagccgt tgggttccat ccgatcgatc gagatgacgc cgaggacccg atcgccggcg
    13141 aggatcggca cccccagcca ggattcgttc tgctccccgt cgccgatgaa gatcgcgccg
    13201 cgatcccgcg catcggccat gctgccgatc cggatcggct gtcgcgacga gatcaccttc
    13261 ccggtgaggc cggtgcccac ctcgaccggc tccatggcga gccgttcgcc gttgtcgacg
    13321 ttgaagggga agctcaggag tcgggtctcc tcgtcgtaca gcgcgatccc cccgccgcgg
    13381 acgccgaaga tcgagaggac gcgctcaccg acgagatcga tgatcgcgtt gaagtcgagc
    13441 tgtcgcgcca gggccgtccc gatctcgttg acgaccgcaa gctccgcgtt gcgctgccgc
    13501 gtctcctcga gggcacgcgc acgggtgagc gccgtggcga tgtgctgggc cacgaaggtc
    13561 aggagctcga ggtcttcggg ggtgtggcgt cgctcctcgt cgtagctctg gaccaccacg
    13621 acgccgatcg tgtgtccgtc cgcctggagc ggagcggcca tccagtcggc gctgggttcg
    13681 ccgagcatgg tgacctcgcc gcgctccacc atggcctcga gttcaccgag ggagagcagc
    13741 accggacgcc cggtccgcag gacccagccg gtgaagccgg ccgcctgccc ttcgcccagg
    13801 ggctcccaga cgttcggatc ggggacgtcc aggtccacct cgtcccggta gaaggggtag
    13861 ttcatcatcc ggcgccgttc gtcgtacagc gcgatgaaca ggttgtcggc gtacatcagc
    13921 tcgccgacga tgcggtggat ctcctcgtag aaggccggca tgtcgcgagc ggcgcttgcg
    13981 gtctccgcga tccggtacag cgcggcctgg atccgctcag agcgctccag ggcggctgcg
    14041 cgctcctcga gctcggtgat gcgccgacgt gcggcgccca ggacctgccg gccctggccg
    14101 tcgacggacg gcggaggttc actccctcgc ggctcgggaa tcggaacggc cctccggtgg
    14161 gcgccaggcg ccgggcaggc gtcggtggcg cgcgcgtatt gtgcgcgaac acgatgcggc
    14221 gcgtcaccaa ccgcgcgcgg atgaggtgga cccatgaacg acagctacac gtacctggac
    14281 gacctggccg ccgaggcgag catcccgaag cgcgggatcc acagccagac cctctccgac
    14341 gccgacggcg tcgagctcgt gctgttcgcc atggcggccg ccgagcggct ctcggagcac
    14401 acctccgccc gctccgcgat cgtccacgtc ctcgccggct cgggcgacct cgtcgtggcc
    14461 ggcgacgcct accggctctc accgggctcg tggatgcgga tgcgggcgcg cacgccccat
    14521 gcgatcacgg ccggggacga agggctcgtc ttcgcgctgt acctcctggc ctcgtcctga
    14581 gccgaggcgt cagggttcga cgggcgcccc cgcggccacg aggtcggaga agccgcccgc
    14641 gtcgatgacg tcggtgaagc caagctccgc catgacggcc gcggcctgcg ccgaacggtt
    14701 tccggtgcgg cagtacagca ggtacttgcc gttccgatcg agctggctga tgcgctcgcg
    14761 gaagtcagcc gcctcgaagt cgatgttcag cgcgcccgcg atcatcccgt ccgcggtctc
    14821 gtcgggggtg cgcacgtcga tcaggacccg cgacggcacc tccgcgaccg cctccgcggc
    14881 cggcagctga cgcaccgcgg cgtcgcccgg cgcgacggcg gtcccgccac aaccgcccag
    14941 caggattgcc agggtcagcg ccatgatggc gcggcgtggg tgcatgtggc cgactacctc
    15001 cgttccgccg ggacgccgag ggtgacggcg gcggcgaccg tcgcggtggc gagcgcgagc
    15061 gcctgccccg cgctgagcgc cgcgtcgccc gcgaagagct gcgtcgcctg gctcgggatg
    15121 gcggccagca cgacgggcac gagcgtccag cccagcacca gcaccgtggg ggcggtggct
    15181 ccgggcagtc gcagcatccg cgtcgccacg gcggtgagga cgccgaggaa ggcggacagc
    15241 agcaggatga ggatcaccgc cggttggtcg cgggcgcccg aggcgtcgac cagtcgtacg
    15301 accgcctggt aggccagggc gaccgccagc gccaggaggg cgccgagcac cccggcccgc
    15361 gcaccgatca tgcgccggac tccccggcca ggcggtcccg gaggccccgg gcgatgcggt
    15421 acgacttccc ggcccccatc accagcaccg catcgccgtc cgcgaggtgc tcggcgacgt
    15481 aggcggtcgt cgcatcgacg tcgcccgttg ccagggccgg aatgccgctg gcccgctcga
    15541 tcgcatcggc gaggtcggcg gccgaggtgg cggccatcgc ctccggcgtg tcgcggctgg
    15601 cgaagatgtc ggcgatgatg acctcgttcg catcggtgaa ggcggtcgag aactcgtcca
    15661 tcagcagcgc cgttcgcgag tacatgtgcg gctcgaagac ggcccacagg cgacgtgtcg
    15721 gcccgacctt ctggcgagcg gcggagagcg ccgcgcgcac ctcggtggga tggtggccgt
    15781 agtcgtcgta gatcgtcacg cgcggagtat cggcgatcag ctccattcgc cgcccggctc
    15841 ccgcgaacgt ttcgagcgcc atcgccagcc g
//
LOCUS       DASTYP010000248         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1050735, whole genome shotgun sequence.
ACCESSION   DASTYP010000248 DASTYP010000000
VERSION     DASTYP010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050735"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFV59_10745"
     CDS             <1..814
                     /locus_tag="VFV59_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5326349.1"
                     /translation="EWTGDKESMIQKHEKYAREAAAQGAKVMCFQELFYGPYFCQVQD
                     PQYFSYTEHIPDGPTTKRMQELAKETGMVLIVPMYEEDEQTTGIYYNTAAVIDADGTY
                     LGKYRKTHIPHVKGFWEKYYFRPGNTGYPVFDTAVGKVGVYICYDRHFPEGARALGLN
                     GAEIVFIPSATSRGLSEYLWRVEQVSHALANGYFVGTINRVGIEPLGDDDFYGQSYFV
                     DPRGQFVGDVGDPHDEELIVRDLDLDKIEEVRQTWQFFRDRRPDAYEDLVRP"
     gene            825..1238
                     /locus_tag="VFV59_10750"
     CDS             825..1238
                     /locus_tag="VFV59_10750"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5326350.1"
                     /translation="MTQRIDPSVVRADARSAATNLPADSFGEEAFNHPGAWVGYLRLP
                     PGATSGWHHHGEWDSYACVLEGVLRWEHGPGGADAIEIAAGDTGHMPAWAVHRDVSAG
                     DTDLVMVLFRAGHGEITINVPGPDPADAGEGGAEA"
     gene            1235..>2012
                     /locus_tag="VFV59_10755"
     CDS             1235..>2012
                     /locus_tag="VFV59_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU5326351.1"
                     /translation="MKFGITLKPDHRFDRSVDLAKRAEANGFSYGWIFDSHILWRDPY
                     PLLTLIGAATERMRLGTCVTNPATRDVTVTASVLATLNEITDGRMDLGIGRGDSARRV
                     MGKKPTTVAYMEECCRIVRGLTAGEVVEIDGTPIQMPWVSHGPVPVWIAGYGPMALAA
                     AGRVADGIILQLADPDIIRWCTGFVRAAAEEAGRDPDAIEVMAAAPAYVSDDTDLARD
                     KVRWFPALVSNHVVDLINRYPKDDLPEALWKYVENREGYDY"
BASE COUNT          346 a          696 c          688 g          282 t
ORIGIN      
        1 ggagtggacc ggcgacaagg agtcgatgat ccagaagcac gagaagtacg cgcgcgaggc
       61 cgcggcccag ggcgcgaagg tgatgtgctt ccaggagctg ttctacggcc cctacttctg
      121 ccaggtccag gacccgcagt acttctcata caccgagcac atccccgacg gcccgacgac
      181 gaagcggatg caggagcttg cgaaggagac ggggatggtc ctcatcgtgc cgatgtacga
      241 ggaggacgag cagaccaccg ggatctacta caacaccgcc gcggtcatcg acgccgacgg
      301 gacgtacctg ggcaagtacc gcaagacgca catcccgcac gtgaagggct tctgggagaa
      361 gtactacttc cgcccgggga acacgggcta tcccgtgttc gacaccgcgg tcggcaaggt
      421 cggcgtctac atctgctacg accgccactt cccggagggc gcgcgcgcac tgggcctcaa
      481 cggcgccgag atcgtgttca tcccgtccgc cacgtcgcgc ggcttgagcg agtacctgtg
      541 gcgcgtcgag caggtcagcc acgcgctggc caacggctac ttcgtcggga ccatcaaccg
      601 cgtcgggatc gagccgctgg gcgacgacga cttctacggc cagtcctact tcgtcgatcc
      661 gcgtggccag ttcgtcggcg acgtgggcga cccgcacgac gaggagctga tcgtccgcga
      721 cctggacctc gacaagatcg aggaggtccg ccagacgtgg cagttcttcc gggatcggcg
      781 cccggacgcc tacgaagacc tcgttcgccc gtaggcgcgc cgtcgtgacc cagaggatcg
      841 acccgagcgt cgtccgcgcc gatgcgcgca gcgccgcgac gaacctgccg gccgacagct
      901 tcggcgagga ggccttcaac caccccgggg cgtgggtcgg ctacctcagg ctgccgcccg
      961 gcgccacgtc cggctggcat caccacggcg agtgggacag ctacgcctgt gtgctggagg
     1021 gggtcttgcg ctgggagcac ggccccggtg gcgccgatgc gatcgagatc gcggcggggg
     1081 acaccggaca catgcccgcc tgggccgtcc atcgcgacgt cagcgccggc gacacggacc
     1141 tggtgatggt cctcttccga gccggacacg gcgagatcac gatcaacgtc cctggccccg
     1201 acccggcgga cgcgggcgag ggaggcgccg aggcatgaag ttcggcatca cgctgaagcc
     1261 cgaccatcgg ttcgaccggt cggtcgacct ggcgaagcgt gccgaagcca acggcttcag
     1321 ctacggctgg atcttcgact cgcacatcct gtggcgcgat ccctacccgc tcctgacgct
     1381 catcggtgcg gcgacggagc gcatgcgcct gggcacctgc gtgacgaacc ccgccacccg
     1441 cgacgtgacg gtgacggcca gcgtcctggc cacgctcaac gagatcaccg acgggcggat
     1501 ggacctgggc atcggccgcg gcgacagcgc ccgacgcgtg atgggcaaga agccgacgac
     1561 cgtggcctac atggaggagt gctgccgcat cgtgcgcggc ctgacggcgg gcgaggtggt
     1621 cgaaatcgac ggcacgccga tccagatgcc gtgggtgtcg cacgggccgg tcccggtctg
     1681 gatcgccggc tacggaccga tggcgctggc ggcggcgggc cgcgtggccg acggcatcat
     1741 cctccagctc gcggacccgg acatcatccg ctggtgcacc ggcttcgtgc gcgccgccgc
     1801 cgaggaagcc ggtcgcgacc cggatgccat cgaggtcatg gccgccgccc ccgcgtacgt
     1861 atccgacgac accgacctcg cccgtgacaa ggtccgctgg ttcccggcgc tggtcagcaa
     1921 ccacgtcgtc gacctcatca accgctaccc caaggacgac ctgcccgagg cgctgtggaa
     1981 gtacgtcgag aaccgcgagg gctacgacta tt
//
LOCUS       DASTYP010000249         8440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1597959, whole genome shotgun sequence.
ACCESSION   DASTYP010000249 DASTYP010000000
VERSION     DASTYP010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597959"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFV59_10760"
     CDS             complement(<1..231)
                     /locus_tag="VFV59_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326352.1"
                     /translation="MEQAAILDSLIGLVQRQNVRVLGMRTDVLVGVLVRARQLPGRPV
                     PDALIVAASLGADALPLATFDRDQRRYGIATLD"
     gene            270..656
                     /locus_tag="VFV59_10765"
     CDS             270..656
                     /locus_tag="VFV59_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5326353.1"
                     /translation="MSIELNHTIVYSRDKHEGARFLTDLFGLPPASVWGPFATVELAN
                     SVTLDYLDAEGDIAAQHYCFLVAESEFDAIFGRITERGLPHWADPGHRQANEINTHDG
                     GRGVYFDDPSGHILEIITRPYGSERG"
     gene            complement(697..1095)
                     /locus_tag="VFV59_10770"
     CDS             complement(697..1095)
                     /locus_tag="VFV59_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5326354.1"
                     /translation="MKQRKAGVTRKPPEPSTSHAEIEEWMGSVMPALQPIVCSLDELI
                     RETIPAVRYAIKWKTPYYGLPERGWLIQLVAYDVSVNVVFFGGADFASPPPLGDTDRS
                     RYVKVTTLDEAQRPELREWVRQAAGVPGWT"
     gene            1212..1508
                     /locus_tag="VFV59_10775"
     CDS             1212..1508
                     /locus_tag="VFV59_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326355.1"
                     /translation="MTTPADAWNRAAAALPAGWRLEGLRCTSTGLDPSQRGDGWLAEA
                     CGPGDECIKVGARDPLDALVTLAERLLEPRQTGKHPRPNESERNSALLSHGQEH"
     gene            1505..2164
                     /locus_tag="VFV59_10780"
     CDS             1505..2164
                     /locus_tag="VFV59_10780"
                     /inference="COORDINATES: protein motif:HMM:NF040123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HEU5326356.1"
                     /translation="MTETVTRGSPGRPDWAVLALIVAGGAVSGVAAKLADESAITGMA
                     DLGTSPTIWVLSLAVIARFAPTEAQAALRAAAFFVAMSIGYYAWTSLVLGYPGSGPLL
                     LRWVGVAFTLVPLVAALVRWACHQSGAGSGLVLAAAASLALADPRVRQLWYAAVGDLP
                     SDFPLRPVQAVVSIATALVITLWLPRRAATRAWAGALLVPVTLLGSAALSGAPGNLLP
                     S"
     gene            complement(2218..2565)
                     /locus_tag="VFV59_10785"
     CDS             complement(2218..2565)
                     /locus_tag="VFV59_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326357.1"
                     /translation="MQEDQVRRNTQGMDDLDFDGWNKADWDGVFADHHTEDVLVDWKG
                     QPTTRGRQAHIDAMKAYVESAGGTPPQITSHPILFGSGEWTCAVGEFADGSRMVTVAK
                     WRDGAIVEEYIWA"
     gene            complement(2744..3436)
                     /locus_tag="VFV59_10790"
     CDS             complement(2744..3436)
                     /locus_tag="VFV59_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326358.1"
                     /translation="MSVSQRRVLRATAIAAAALLATAAAWNLLFQVRIVTPPLPSADN
                     AHYYAWWRPIEWQTFAIALLAAAGLGGVGVVGLSLRGAAGAVLGIGAIAAMVAQLAQA
                     GAQHAILAASTSAIDPGVLGTIGFVADGIVNGVSIGAYAGMALGVVGLAVSGEDALPG
                     HAAGRLTGALLGAGLVALSVFSLADPLDLLQPVLGIVGMVLTPAWLWQVGASLSASGA
                     RAGDRAPVAGAA"
     gene            3611..3964
                     /locus_tag="VFV59_10795"
     CDS             3611..3964
                     /locus_tag="VFV59_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5326359.1"
                     /translation="MRRTVLVVDDHAGFRASLRELLAIGPFEVVAEAADSEAALEAFD
                     RTRPDVVLLDVALPGTDGFGVAEVLARHDPPPAVVLISTRDAADYGPRIGAAPVRGFV
                     QKAALTVGALEVLVR"
     gene            3961..5736
                     /locus_tag="VFV59_10800"
     CDS             3961..5736
                     /locus_tag="VFV59_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5326360.1"
                     /translation="MRRQRRRVVPALTAVGLAAVVLAEWVSLGAYARVDGDNVAGQLI
                     HDALPPTVYLVAGIAAMHARPESRIGFWLWLAGVLTFTGNFGNALVPGLSQLSIGLQD
                     IYLVPMGIVILTYPMGRLDSPPVRWLIGLAVIQIVAVGLFVTASLDPADCAPGWCPAN
                     PFLLIRDGGTVAAVNVIGQVTGGALWLAMALIVARRYLVASGTARRLLLPVWIAGLLL
                     AASGVASLVVETVRGPAAQYAYDYWVGWVVSMVPPVVFLAGLLRQRLDRAGIATFVEE
                     LANGITVGSLRDAFARLVGDTTLVLAFRMDDGGYADAQGTLVDPSAEGEGRMVTSIER
                     GGREIAVAITDPALQTDPAMLRAAAAAAGLALDNERLTAEVRARVEAIRTSRARLVEA
                     ADAERVRIERMLHDGAQQRLVALALRVRALAAGTDEPAVSMRLDALGADLDEALAELR
                     DLARGIHPAVLVQAGLAAALAALAERSPLPVLVDVPDIRLPAPIESTAYYVAAEALTN
                     ATRHARATSVTISAWLDGATFHMTVLDDGVGGADPRHGSGLAGLEDRVAASAGSLTVG
                     GGPHGGTLVEIRLPIRQPTVAQPAS"
     gene            5733..6386
                     /locus_tag="VFV59_10805"
     CDS             5733..6386
                     /locus_tag="VFV59_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5326361.1"
                     /translation="MKIVIAEDSDLLRETLAGALREHGVEVAATASTESELVSAVEHH
                     LPDAAVVDIRLPPSFTVEGLRATATLRARRPRFPVLLLSQHVEGGIALKVLRDDPAGI
                     GYLLKDRVTRVSQLVAALERLVAGGSVIDPEIVSALLGRPRRHGPLDELTPRERDVLR
                     LMAEGRSNRGIGQELALEPKTVEHHVGQVFSKLGLEPAQHDHRRVLAVLAWLNEPAP"
     gene            6497..7561
                     /locus_tag="VFV59_10810"
     CDS             6497..7561
                     /locus_tag="VFV59_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HEU5326362.1"
                     /translation="MPTTVAAGGAPRWTGRLHADPTPWLLETSVPAVRHRALRELLDL
                     PADHQDVAAARAAAMRTDPIAAILAAQHPDGWWIKPGGGYSPKYTGTIWSLIFLDQLG
                     ADGSDPRVRAACGYVLDHAHAPSGGFGWSGGAAVPAPSTVAHCLNGNVLRALIGFGWL
                     DDERVQRSIDWQASAITGQGDVRWYRTATSGPGFECAVNERLPCGWGAAKSVLALARV
                     PPDRRSPAVTEALRAGVEFLLSVDPSTARYPMGWGNTKPNGSWFRLGFPSGYVADVLQ
                     VLEAVCEAGSGADARLAPAADWLLAQQDANGRWANRYPYAGKMHADIDRPGAPSRWVT
                     LRACRVLKRIDDAQAFRTRR"
     gene            complement(7592..7837)
                     /locus_tag="VFV59_10815"
     CDS             complement(7592..7837)
                     /locus_tag="VFV59_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326363.1"
                     /translation="MEPSEAADELNEVSVLVNVAHRLLVAIREDIESSPSLQGIVDDL
                     DAAHDQLDAALDRLGAAIDVADAEAGAQPREQRTPIH"
     gene            8017..>8440
                     /locus_tag="VFV59_10820"
     CDS             8017..>8440
                     /locus_tag="VFV59_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5326364.1"
                     /translation="MNPGGPDAVSRRRGATRGFLFADLRGYTAYLERRGANEAAELLD
                     RYRKLVRAAVDEHDGAEIRTEGDSFYVVFPDASGAVACGLAIAAAAADASTATPEAPI
                     RVGIGVHAGETVETQEGYVGTAVNLAARLCAAAPAGDVL"
BASE COUNT         1157 a         3018 c         2968 g         1297 t
ORIGIN      
        1 gtccagcgtc gcgatcccgt atcgccgctg gtctcggtcg aacgtggcca gcggcagtgc
       61 atcggcgccc aggctcgcgg ccacgatgag agcgtccgga accgggcgcc cgggaagctg
      121 acgcgcgcgg acgaggacgc ctacgaggac gtccgtccgc atgccgagca ctcgcacgtt
      181 ctggcgctgc accaggccga tgaggctgtc gaggatggcg gcctgctcca ccccgtactg
      241 cgttcaggcg gcctcaacgg agggcgacga tgtcgatcga gctcaaccac acgatcgtgt
      301 actcccgcga caagcacgag ggggccaggt tcctgaccga cctgttcggc ctcccgcccg
      361 catccgtctg ggggccgttc gcgaccgtcg agctggcgaa cagcgtcacc ctggattacc
      421 tcgacgccga gggcgacatc gccgctcagc actactgctt cttggtcgcc gagtccgagt
      481 tcgatgccat cttcggccgc atcaccgagc gcgggctccc ccactgggcc gatcccggcc
      541 accggcaggc caacgagatc aacacccacg acggtgggcg cggtgtgtac ttcgacgatc
      601 cgagtggcca catcctcgag atcatcaccc gcccgtacgg gtccgagcga ggctgaccga
      661 tccgtgatca gctgaggccg atacggtctc ccgcgactat gtccacccgg ggacgccggc
      721 ggcctgtcgc acccattcgc gcagctcggg tcgctgcgcc tcgtcgaggg tcgtgacctt
      781 gacgtatcgg ctgcgatccg tgtcgcccag cggcgggggc gaagcgaaat cggcgccgcc
      841 gaagaagacg acgttgaccg agacgtcgta cgccacgagc tggatcagcc agccacgctc
      901 gggcaggccg tagtacggcg tcttccactt gatcgcgtac cggactgcgg gaatggtctc
      961 gcggatcagc tcgtccaggc tgcagacgat cggctgcagc gccggcatca ccgatcccat
     1021 ccactcctcg atctcggcgt ggctggtcga gggctcaggc ggcttccgcg tcactcctgc
     1081 cttgcgctgc ttcatcgtca acgacggcct cctccatccg gtccctggtt ggacacttga
     1141 gtcattgtcg accagcgggc cccgacaccg ccgtctacaa ctgaaccgtc tggatgcgag
     1201 acaatggagg catgactacc ccggccgacg cctggaaccg cgcagcggct gcgctgcccg
     1261 cgggttggcg gctggagggc cttcgatgca cctcgaccgg cctggatccc tcgcagcgtg
     1321 gcgacggctg gctggctgag gcctgcggtc ctggggacga gtgcatcaag gtgggcgctc
     1381 gtgatccgct cgatgcgctg gtgacgctcg cggaaaggct tcttgagcct cggcagacgg
     1441 gcaagcaccc gcgcccaaac gagtcggaac gcaattcggc cctgttgtca cacggccaag
     1501 aacactgacc gagacggtca cgcgcggcag tccggggcgc ccggactggg ctgtgctcgc
     1561 gctgatcgtg gccggcggcg ccgtcagcgg agtggctgcc aagctcgcag acgagtcggc
     1621 catcaccggg atggccgacc tggggacgtc accgacgatc tgggtgctgt ccttggcggt
     1681 catcgcccgc ttcgccccga ccgaggcgca ggccgccctc cgcgcggctg ccttcttcgt
     1741 cgcgatgagc atcggctact acgcctggac gtccctcgtg ctcggatatc ccggcagtgg
     1801 cccgctgctg ctccgctggg tgggggtcgc attcacgttg gtgcccctcg tcgctgcgct
     1861 cgtccggtgg gcctgccacc aatcgggagc gggctccggc ctggtgctgg cagctgcggc
     1921 aagcctcgcc ctcgccgatc cccgggtccg tcagctctgg tatgccgcgg tcggcgacct
     1981 gccgtccgac ttcccgctcc gtcccgtcca ggcggtggtc agcatcgcca ccgcgctggt
     2041 catcaccctc tggctacccc ggcgcgccgc gactcgagcc tgggccggag ccctgctcgt
     2101 gccggtgaca ctcctcggct ccgccgcgct gagcggcgcg cccggcaacc tgctcccgag
     2161 ctgagatcgg gcccgccgga tgcggcccca tcccggctgg gcacgggcca ggggtggcta
     2221 ggcccagatg tattcctcga cgatcgcacc gtcgcgccac ttggcgacgg tgaccatgcg
     2281 gctgccgtcc gcgaactcac cgaccgcgca ggtccactcg cccgacccga acaggatcgg
     2341 gtgcgaggta atctggggtg gcgtgccgcc ggcggactcg acataggcct tcatggcatc
     2401 gatgtgcgcc tgccgtccgc gggtcgtcgg ctgtcccttc cagtcgacca ggacatcctc
     2461 cgtgtggtga tcggcgaaca cgccgtccca gtcggccttg ttccagccgt cgaagtcgag
     2521 atcgtccatc ccctgcgtgt tgcgtcgaac ctggtcttcc tgcacgagag tgtctccttc
     2581 cgggcgccgt tcggagccgc gccgcgctca gcggcgggcg cgggatgcag tgcacgccgc
     2641 gtacctcgcg ccctccccgc ccagtcggag gcctcgctcg gtggacctcg tgccgggcgt
     2701 cgacccgccg gatagggagc tgctccgcga ggcgccctgg cgctcacgcg gccccggcca
     2761 ccggtgcacg atccccggcc cgcgcgccgg atgcggagag ggaggcgccg acctgccaaa
     2821 gccaggccgg cgtcaggacc atgcccacga tcccgagcac gggctgcagc agatcgaggg
     2881 gatccgcaag cgagaagacg ctcagggcga ccagccccgc ccccagcagg gccccggtca
     2941 gccgcccggc cgcatgaccc ggcagagcgt cctctccgct caccgccagc cccacgacac
     3001 cgagcgccat gccggcgtag gcgccgatgg agacgccgtt gacgatcccg tcggcgacga
     3061 agccgatcgt ccccagcaca cctggatcga tggccgacgt cgaggccgca aggatcgcgt
     3121 gctgcgcgcc ggcctgcgcc agttgcgcga ccatcgcggc gatcgcgccg atgccgagta
     3181 ccgcaccggc ggcaccccgg agggacagtc cgacgacgcc gacacccccg aggccggcgg
     3241 cggccagcag tgcgatggcg aaggtctgcc actcgatcgg ccgccaccac gcgtagtagt
     3301 gcgcgttgtc ggctgaaggc aggggtgggg tcacgatgcg cacctggaac aggaggttcc
     3361 aggccgcggc cgtggccagg agcgcggccg ctgcaatggc ggtcgcgcga agaactcgtc
     3421 gctgtgagac ggacatgaga acgctccttc cctgatcccg gcggctggcc gccattgcgc
     3481 aggcgccgcc atggccatga gggtgagcga ccgatccacg cgggtcgccg gggcagaccc
     3541 gcagccgcgg cacaggaagt cgagggtttt ccccgaggcc gccccttgcg gcatgcggga
     3601 cgatgtcggc atgcgccgga cagtgctggt cgtggacgac cacgccggct ttcgcgcgtc
     3661 gcttcgcgag ctgctcgcca tcggaccgtt cgaggtggtc gccgaggccg ccgattcgga
     3721 ggcggccctc gaggccttcg acaggaccag gcccgacgtg gtgctgctcg atgtggccct
     3781 tccgggcacc gatggcttcg gcgtcgccga ggtgctggcc cgccacgacc cgccgccggc
     3841 cgtcgtcctg atctcgaccc gggatgccgc cgactacggg ccgcgcatcg gcgcggcgcc
     3901 cgtgcgtggg ttcgtccaga aggcagcgct gacggtcgga gcgctggagg tcctggtgcg
     3961 gtgaggcggc agcggcgtcg tgtggtcccc gccctgacgg ccgtggggct cgcggcggtc
     4021 gtgctagccg aatgggtgtc gctcggcgcg tacgcgcggg tcgacgggga caacgtcgcc
     4081 gggcagctga tccacgacgc gctgcccccc accgtctacc tggtcgccgg catcgcggcc
     4141 atgcacgcga ggcccgagtc tcgcatcggc ttctggctct ggctggccgg cgtcttgacg
     4201 ttcaccggga acttcggcaa cgccctggtg cccggcctga gccagctgtc gatcgggctc
     4261 caggacatct acctcgtgcc gatgggcatc gtcatcctga cgtaccccat gggccggctc
     4321 gactcccctc cggtccgctg gctgattggt ctcgccgtca tccagatcgt ggccgtcggc
     4381 ctgttcgtga cggcgtccct cgatccggct gattgtgctc ccggctggtg cccggcaaac
     4441 ccgttcctgc tgatccgcga cggtgggacg gtggcggcgg tcaacgtgat cggccaggtc
     4501 acgggcggcg ccctgtggct ggcgatggcg ctgatcgtcg cgaggcgcta cctggtcgcc
     4561 agcgggaccg cacggcgcct cctgttgccg gtgtggatcg ccggcctcct gctggccgcc
     4621 tccggcgtgg cgtcgctcgt cgtcgagacg gtccgcgggc cggcggcgca gtacgcgtac
     4681 gactactggg tcggctgggt ggtgtcgatg gtgccacccg tggtgttcct ggcgggactc
     4741 cttcgtcagc gcctggatcg ggcggggatc gcgacgttcg tggaggagct cgcgaacggg
     4801 atcacggtcg gaagcctgcg tgacgcattc gcgcgcctgg tcggagatac gacgctggtg
     4861 ctggcgttcc gcatggacga cggcggctac gccgatgcgc agggcacgct cgtcgatcct
     4921 tccgcggagg gcgagggacg catggtgacg agcattgagc gcggcggccg tgagatcgcg
     4981 gtcgccatca ccgacccggc actccagacc gatccggcga tgctgcgggc agccgcggcg
     5041 gcggctgggc tggcgttgga caacgagcga ttgaccgcgg aggtgcgcgc acgagtcgag
     5101 gcgattcgga catcacgagc tcggttggtg gaggcggccg acgccgagcg ggtccggatc
     5161 gagcgaatgc tgcacgatgg cgcgcagcaa cgactggtgg cactggcgct ccgtgtccgg
     5221 gccctcgccg ccggaacgga cgaaccggcg gtcagcatgc gcctcgatgc gctcggcgcc
     5281 gacctcgacg aggcactcgc cgagctgcgt gacctggcgc gcgggatcca tcccgccgtc
     5341 ctcgtccagg ccgggttagc cgctgcgctc gccgcactcg ccgagcgcag cccgctcccc
     5401 gtcctcgtcg acgtcccgga catccggctg cccgcgccga tcgagtcgac cgcctactac
     5461 gtcgccgccg aggcgctgac gaatgccacg cggcatgcgc gtgcgacgtc ggtgacgatc
     5521 tccgcatggc tggacggcgc gaccttccac atgaccgtgc tcgacgatgg cgtcggtggt
     5581 gccgacccgc gccacggttc cgggctcgcc ggcctcgagg atcgcgtcgc cgcatccgcc
     5641 gggtcgctca ccgtcggtgg cggtccgcac ggcggtaccc tcgtggagat caggctgcca
     5701 atccggcagc cgaccgtagc gcagccagcg tcgtgaagat cgtcatcgcc gaggactccg
     5761 acctgctccg ggagacgctt gccggcgccc tccgcgagca tggcgtggag gtcgcggcga
     5821 cggcctcgac ggagtcggag ctcgtgtcgg ccgtcgagca tcacctcccc gatgccgcgg
     5881 tcgtcgacat ccggctaccg ccctcgttca cagtcgaagg gctgcgagcc acggccacgc
     5941 tccgcgcgcg gcggccacgg ttcccggtcc tcctcctgtc gcagcacgtc gaaggaggca
     6001 tcgccctcaa ggtgcttcgc gacgatccag ccggcatcgg ctacctgctc aaggaccgcg
     6061 tgacccgtgt gtcccagctg gtggccgcac tggaacgcct ggtcgcgggc ggctcggtca
     6121 tcgatcccga gatcgtctcc gcgttgctgg ggcgaccacg ccgccacggg cccctcgacg
     6181 agctgacgcc acgggagcgc gatgtgctgc ggctcatggc cgagggtcga tcgaaccgag
     6241 gcatcggcca ggagctcgcg ttggagccga agaccgtcga gcatcacgtc ggccaggtct
     6301 tctccaagct tgggctcgag cccgcccaac acgaccatcg tcgcgtgctg gccgtcctgg
     6361 cctggctcaa tgagccggcg ccgtgacccc gcatcgcggg acgacatcga catgggcgtc
     6421 acgttcgcgc aaccggcttt ccgccgcacg acatctggca ctggctcctc gcggggtcgg
     6481 ccaccagcat cggaccatgc ccacgaccgt ggctgcaggc ggcgccccgc gatggacggg
     6541 ccgactccat gccgatccga cgccctggct cctcgagacg tccgtgccgg cggtccggca
     6601 tcgcgcgctc cgcgagttgc tcgaccttcc cgcggatcac caggacgtgg cggccgcgcg
     6661 ggccgcggcc atgcgcaccg acccgatcgc ggcgatcctc gcggcgcagc atcccgacgg
     6721 ctggtggatc aagcccggag gcggctacag cccgaagtac accggcacca tctggtcgct
     6781 catcttcctg gaccagctcg gcgccgatgg gtccgaccct cgcgtccggg cggcctgcgg
     6841 gtacgtgctg gaccatgccc acgcgcccag cggcggcttc ggctggtccg gcggggcggc
     6901 ggttccggca ccgtcgacgg tcgcccactg cctgaacggg aacgtgctgc gggcgctcat
     6961 cggcttcgga tggctcgacg acgagcgcgt ccagcgctcg atcgactggc aggcgtccgc
     7021 gatcacgggt cagggcgacg tccgctggta ccgcaccgcc acgtccggac cgggcttcga
     7081 gtgcgccgtg aacgagcgcc tcccctgcgg ctggggcgcg gccaagtcgg tgctcgcgct
     7141 ggctcgtgtg cctcccgacc gccggtcgcc tgcggtgacc gaggcgctcc gcgcaggggt
     7201 cgagttcctc ctctcggtcg acccatcgac ggcccggtac ccgatgggct ggggcaacac
     7261 gaagccgaac ggcagctggt tccgcctggg cttcccgagc ggctacgtgg ccgacgtgct
     7321 ccaggtgctg gaggcggtgt gcgaagccgg ctcaggagcc gatgcacgcc tggcgccggc
     7381 ggccgactgg ctgctggcgc agcaggacgc gaacggccga tgggcgaacc ggtacccgta
     7441 tgccgggaag atgcacgccg acatcgatcg gccgggagca ccgagccgat gggtcacgct
     7501 gcgcgcctgc cgcgtcctga agaggatcga cgacgcccag gccttccgga cgcggcggta
     7561 gcctcgccga agaggaccga gcgccccgag ctcagtggat tggcgtacgc tgctcgcgtg
     7621 gctgggcgcc ggcctccgca tcggcgacgt cgatcgctgc cccgagccga tccagcgccg
     7681 cgtccagctg gtcgtgggcg gcgtccaggt cgtcgacgat gccctgcagg gacggcgagg
     7741 actcgatgtc ctcgcggatg gcgacgagta gccgatgcgc gacgttgacg agcaccgaca
     7801 cctcgttcag ctcatcggcg gcttccgacg gttccattgg gacgttcctc cattggtctg
     7861 aggtgacgga ctcgaagcgc tgattagtct gcatcaccga tcggcccatc gaggtcggta
     7921 ggcggatcga ggctggtccg ggttgcaatg ggaagaggct catcggcacc gtgtcgtcac
     7981 gttgggcgac cttgaccccg ctgcgatcat tcggccatga atcccggagg gcctgacgcg
     8041 gtctcccggc gccggggggc gactcgggga ttcctcttcg ccgacctgag gggctacacc
     8101 gcgtaccttg agcgccgtgg cgccaacgag gccgccgagc tgcttgatcg ctaccgaaag
     8161 ctcgtgcgcg ccgcggtcga cgagcacgac ggcgccgaga tccgcactga gggcgactcg
     8221 ttctacgtcg tcttccccga cgcgagcggg gcggtggcgt gcggtctcgc gatcgcggcc
     8281 gcagcggccg atgccagtac cgcgacaccg gaggccccga tccgcgtcgg aatcggtgtc
     8341 cacgccgggg agaccgtcga gacgcaggag ggctatgtcg gtaccgcggt gaaccttgcg
     8401 gcccgtctgt gtgccgcggc gcctgccggc gacgtcctcg
//
LOCUS       DASTYP010000250        18901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_721956, whole genome shotgun sequence.
ACCESSION   DASTYP010000250 DASTYP010000000
VERSION     DASTYP010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 18901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18901
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721956"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VFV59_10825"
     CDS             complement(<1..1271)
                     /locus_tag="VFV59_10825"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEU5326365.1"
                     /translation="MTTITMPRRLLRPMGSPPARRSMLIGFAGTLLLGLAMLAGTAVA
                     IGAANAGAVMPGVSVAGVDLAGLTQDDARARLADELPALDRGTVRVTVGDREASVAFA
                     DLGRGYDLDRMAAAAFDVGRDDNPFLAGLERLRSVVHRAALPVAVHPYDPEALDAAAG
                     DVADELSVAPVDAALVQDGARFTFTPSRDGEVVDAEAVKAALARAADTTRPGDVAIQL
                     SATTTAPAITTDEARAAAVDATASVRPLDLTIPGAAEDEAPIAWSSETLAGLLDVTTD
                     AETVQVAVDTAALTDLVSGLVEQIDQAATNARITVASGGGLGGIVPGAPGRALDVDAS
                     VASLVDAFTRRADGEEVRSVPLVVDVTEPQFTTAQAEAVLPRMQMVSSWTTYYVPGDG
                     NGWGNNINIPARDIDGRNLYPGEWFSFWDSIG"
     gene            1552..1830
                     /gene="rpsT"
                     /locus_tag="VFV59_10830"
                     /pseudo
     CDS             1552..1830
                     /gene="rpsT"
                     /locus_tag="VFV59_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
     gene            complement(2158..3447)
                     /gene="eno"
                     /locus_tag="VFV59_10835"
     CDS             complement(2158..3447)
                     /gene="eno"
                     /locus_tag="VFV59_10835"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU5326366.1"
                     /translation="MTSSLIEDVIGREVLDSRGNPTVEVDVLLEDGSVGTAIVPSGAS
                     TGAHEAVELRDGDGDRYGGKGTLTAVKNVNDTLRPELIGVDAVDQVSVDRLLIALDGT
                     DNKGKLGANALLGVSMAVAHAAAESVGLPLYRYLGGAGARTLPVPLVNILNGGKHAIN
                     STDFQEFMIAPLGAPSFREALRWAAETFHALGKILHDEGFATTVGDEGGYAPSLGSNE
                     DAIAAVLAAIERAGYTPGEQIAIALDPATTELYGDGHYTLAREDRSMTSEELVEFWAG
                     WVERYPIVSLEDGLAEDDWDGWRALTERVGERIQIVGDDLLVTNTERLARGIEQRAAN
                     SILVKLNQIGTVTETIEAIEMAQRAGWTAVISHRSGETEDTTIADLAVALNTGQIKTG
                     SISRSERIAKYNRLLRIEEELADAAVFAGRGAFRQRR"
     gene            complement(3444..4652)
                     /locus_tag="VFV59_10840"
     CDS             complement(3444..4652)
                     /locus_tag="VFV59_10840"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5326367.1"
                     /translation="MDYKTVRDVDVDGKRVFLRADLNVPLDDGRITDDTRIRASLPTI
                     VHLLDRGATVILASHLGRPKGKVNDALRLKPVADRLSQLLGRPVRMTGDALGPGVQVA
                     VDKLRPGDMILLENLRFHAEEEANDPEFAKSLAGLADLYVNDAFGAAHRAHASTEGIT
                     HYLPSVAGLLMEREVDALQTLLERPAKPFHAVIGGAKVSGKLEVLEALLSRCQAILVG
                     GGMANTFLASKGLALGKSLVEEEQLDNATRIVEEARRKRVRLMLPTDAVIAPQIHHRA
                     KTKVVPVDAVPKDQMIVDIGPETVAAYTEHLAKAKTVFWNGPMGVFEHPQFAEGTRAI
                     ARSLATLTDQGAVTVVGGGDSVAAVEQLELTDRMTHVSTGGGASLEFLEGKTLPGVAA
                     LLRREAEVPA"
     gene            complement(4671..5681)
                     /gene="gap"
                     /locus_tag="VFV59_10845"
     CDS             complement(4671..5681)
                     /gene="gap"
                     /locus_tag="VFV59_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5326368.1"
                     /translation="MTTRIGINGFGRIGRQSLRAILERHPDELEVVAVNDLAPTRTNA
                     HLFKYDSTYGTFEGDVTAGENTITVDGHEIRAFSEKDPAALPWKDLQVDIVIESTGIF
                     TDATKARAHVEAGARKVIISAPAKNEDVTLVLGVNEDKYDPEQHHIVSNASCTTNGLA
                     LPAKVVFDTFGIERGLMTTIHSYTNDQNVLDVFHKDLRRARSAGQNIIPTTTGAAKAL
                     ALVIPELKGKFDGFSLRVPTPTVSVIDFVAITEKPVTAESANDALRAAAEGPMQGLLG
                     YTTEELVSMDFKGDSRSSIIDAASTMVTGDRLLKVIAWYDNEWGYSCRVADLAAFMAE
                     RL"
     gene            complement(5678..6724)
                     /locus_tag="VFV59_10850"
     CDS             complement(5678..6724)
                     /locus_tag="VFV59_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5326369.1"
                     /translation="MELAAERPVLAVDLGGTQIRAALITPDREVRHRRAVKTRDEDGV
                     TAVLDRIVEIAAAVRDDARRAGDPEPVGLGISAPGPLDRWRGIVLAAPNLHGWENVSV
                     VDHVAPRIGLATYLERDTNVAVMAEWRYGAGRGAQNVIYITVSTGIGGGIVLDGRPII
                     GPDGSAGEVGHMTVNLDGPICGCGGVGHAEAIGSGTALAREARALLDAGRAPVLARLA
                     ADGREPDAELLAAAAHAGDDDAGQVLERAWEAIGALCASLVNVLDPEVIVLGGSIAQH
                     QPRLFEVIDAELERRAFAFLRHRVRIERAALGGDVSLIGLLPIVNDRIHDAAFRAAAS
                     RPPSAASQQGASPS"
     gene            complement(6880..7023)
                     /locus_tag="VFV59_10855"
     CDS             complement(6880..7023)
                     /locus_tag="VFV59_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEU5326370.1"
                     /translation="MATEAYCVKCKTKREMQNAEKITMKNGKPATQGVCPVCGTKMFK
                     IGG"
     gene            complement(7051..7923)
                     /locus_tag="VFV59_10860"
     CDS             complement(7051..7923)
                     /locus_tag="VFV59_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU5326371.1"
                     /translation="MTGVTQADAPMTGTRQYHIALERGELAEYILLVGDPGRVAKVAE
                     RWDGVEVERSNREITTATGTYRDMRVSCMSTGMGTDNVEIVLAEVMEITDRPTLIRIG
                     SSGALQPEIALGDLIVSTGAVRLENTTDFYVHPGFPAIAHRDVVWALEQACRDQGVRY
                     HVGLTATASGFYAPQGRAMRTLPVRYPALADELRRQRVANLEMESSALFVLAHLAGLR
                     SGTVCAAYAQRTDGTFLEGAAKEAAEARCIDAGLAGIHLLWQADTDAGDTLASRRPSG
                     TGQEGSSQAPTATE"
     gene            complement(7987..11004)
                     /locus_tag="VFV59_10865"
     CDS             complement(7987..11004)
                     /locus_tag="VFV59_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326372.1"
                     /translation="MATVPAARSPGFVGLLVSVLVVATLPLATLTAVAAAPDHLLISE
                     VVTGGASASDELIEIYNPTATPLPLEGLELVYVSASGATVSRRAAWALGAPPVEPQAH
                     LLIANEAGVYASIADVTYATGMAATGGSVALRIQGATTAIDAVGWGTATGTWLEGVPA
                     PAPAAGSSIERLPGGALGSTQDTDDNATDFALLTVPDPQNRASLPTPAPGGSPTATPS
                     STPTAEPTPEPSGTPSATTSPTPDPTPTSTPPPTPTPTSTPTPSGSPSPSPAPVVDVA
                     VARGLPDGTSVTIEGVALTDSAFSDGGGYVADASAGIAVLVSGGAISRGAHVRVTGSL
                     DDRYAQRTIRADALDVVTLGTGDAPPATAVASGQIGEAFEGSLVRVTATVVDGPTMLS
                     SGPAYDVDDGTGVARVLVATSTGIDTTTWSVGTSLDLVGVVGQRDSSGTGAAGYRLQP
                     RDPADVVSVTPAPSPIPTPSPSPTSGATATPGGSAVPTPAPGTMSIAEARQASKNAAV
                     RVRGVVTLPSGLVDDSTAVVQDATGAIVLRLGDDAGALTRGRLVDVSGARSTKGGMET
                     VRVTVPPSDLGPATEPAPRAVRTGDVGEPLEAQLVLVRGAVSSPRHSSNGTVSFDVDD
                     GTGPLRVVATSAVGVDAELNAGDWIEVVGVVGQETTGAQPLRGYRLWPRDPSDLRVTA
                     SAGDADGADGASGSNGDGRGGGGADRDATSRGGTVSLRSLDRAAAPHDLEIGATLVAA
                     RWPELGVAGLLWDGERLVAIDDQAAAAIARSLPRVTVPLPLRLTGLSGAGRHEATGIA
                     IVTLGSGHVASGGALAAPVTQLPGAGEAPRWVTVVGRLIRGATDDRAAVMLAGRGPVT
                     VVNACAARTLHAGDRGQVSVTGIGSASPAAITVGCDGIRTAPVLRAGPSLALPGPSDV
                     AVLSTSTAPDASGSGADDSRLAAVLVLLVGLFLVVAAAIARALRLGGEGPELSTTDAD
                     TTHDDDDEDATELEPAVRVPALTLVAVPRERGP"
     gene            11136..11783
                     /locus_tag="VFV59_10870"
     CDS             11136..11783
                     /locus_tag="VFV59_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326373.1"
                     /translation="MSNRTPDDRAASRAEARRRARLAARGELSETEASAEEPAPAASG
                     SGGGFLSRIFPPAPALPGRPDPLAGFDRSGPLRPVRERLFLLRSNLVAWLVTGLVAFI
                     GFYASFAYAGSILGIVGTFVLFGALIGAGWFGWQRPTLFGTAAALVSFVIGAALLIVA
                     FSAADSTIQTADIVASLVPQALYQAVLGFIGGWYGGYLRRRQTQISQQTRPSRRR"
     gene            complement(12022..14829)
                     /gene="carB"
                     /locus_tag="VFV59_10875"
     CDS             complement(12022..14829)
                     /gene="carB"
                     /locus_tag="VFV59_10875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5326374.1"
                     /translation="MTTTTRPPRKVLVIGSGPILIGQAAEFDYAGVQACLALRAAGVE
                     TVLVNSNPATVMTDPDVGGTVIIGPLTLDHVTRVIEQHRPDALLPTLGGQTGLNLAVA
                     LDDAGVLDRYGVRVLGTPLDAVRAAEDRGGFRDLVMRIGEPVPESAVIESVEDGLRFV
                     EMLGAPVVVRPAFTLGGGGGGFAATPDEARDRIAAGLAASPIHQVLVERSLLGWYEIE
                     FEVLRDAADTTIAICGMENLDPMGVHTGDSIVVAPIQTLPDPVVQRLRRCALNIVRAL
                     KLEGGCNVQLAVSPDHADYRVIEVNPRVSRSSALASKATGYPIARIAAQIALGRLLHE
                     MPNPATGGNSAAFEPAVDYVVVKLPRWPFDKFPTADRSLGIQMKATGEAMAIDREFGP
                     ALLKALRSLEPRGRGWLWEDPAWALDERAPDDLEAFLAPSDTRLWRMVALLRHGLADA
                     ADISAATGVAPWFTERIAELVAAEATVVGAPLVDAKRTGFGDADIGQLSNVPAVAIRR
                     ARVRAGIGPAYRRVDTCAGEFPAETPYFYSSYAEPEVAPPAERQSVIVVGSGPIRIGQ
                     GIEFDYCSVRAAWAIRDMGLDAVVVNNNPETVSTDYDASTRLYFEPLDTESVLDVIDH
                     ERALTGTPPAVVLTFGGQTAIDLAKDLAYAHVPIAGLTAEAIEITEDRERFAQLLNQL
                     GLAGPRGSLASDEGSLREAIGALGGLPVIVRPSWVIGGRGIAVLRTDQDVREYLATDV
                     GWPLRVDEMLEGIELDVDAISDGEAWSVPGILEQVDPPGVHSGDSVAVLPPQRLPREQ
                     QERAAEAAGRIALALGVRGILNVQMVVVGERVVVIEANPRASRTVPIVAKATGLDVVA
                     AAVRCALGADLGSVGLSPGLHADGRLVAVKAPVGSLARLPGVSRDLGPEMRSTGEVLG
                     LAPVAADAHRLALEAASAHAS"
     gene            complement(14826..16004)
                     /gene="carA"
                     /locus_tag="VFV59_10880"
     CDS             complement(14826..16004)
                     /gene="carA"
                     /locus_tag="VFV59_10880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5326375.1"
                     /translation="MQTAHHLDLATDSTPSAAPRVASSSSSEPRPGRLALEGGLVLEG
                     RLHGGPAPASGELVFTTASTGWGEILTDPSYAGQIVVLTHPMAGNYRIDRSELESDHV
                     RARALVVTRLVEPPPGAGASLSELLTEGGIPAISGVDTRALTLRLRNGAARRALIVDR
                     AVAADDAVEGARDSAPWDSVDHVGVVATSRPYVVAPQGEVRGRATLVDYGVKRSLLRA
                     LTQRGLVVTVLPPDATAADVRTTRPDLVVLSPGPGDPSRMAPQIATVRTLAAAAADGG
                     TPILGICLGHQLLALAAGAETHRMPVGHHGGNHAVLDVAAGRVDIGAHNHEVAVLDGP
                     ALAAAGYGISHRDLNDDTVEGMVHASGRIASVQFHPEGAPGPIDAARVFDLATSGATV
                     "
     gene            complement(16066..16983)
                     /locus_tag="VFV59_10885"
     CDS             complement(16066..16983)
                     /locus_tag="VFV59_10885"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEU5326376.1"
                     /translation="MTRRLFALLAVAALLVACGAQGGADFGDVGQPVVPGASAAAGGA
                     DGEAAPDEDRGVAGGGADDGSGSYAVPIEQRIVKTGEVTLEVASVAETLGRVRALVTQ
                     LSGYVGGSQAGTLDDSATITARIPAASFDDALARLHELDGEVIAEATREENVTTQIVD
                     LGARIDNLEASEASYRELVARAEKIEDILAVQSRLDEVRGQIEQLTAQLKALEGQADL
                     STLTITLVPKAEPISQQAETWDPGAQLNAALAALVGIGQGLLNGLIWFGIVWLPILLI
                     LALLVVIALRGVLEVRRRMPAPRRDDAPA"
     gene            complement(17073..17246)
                     /locus_tag="VFV59_10890"
     CDS             complement(17073..17246)
                     /locus_tag="VFV59_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326377.1"
                     /translation="MSRMNFAPRLPTIIVALALVLVGLLGTFGSVLPDAVGVWAFLLA
                     AAALIIGMVVEGL"
     gene            complement(17250..17477)
                     /locus_tag="VFV59_10895"
     CDS             complement(17250..17477)
                     /locus_tag="VFV59_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326378.1"
                     /translation="MLHPNPPRLITVGIAVFLLLVGISATIFPIDIVNEALGMVQAQV
                     GTNIEVTSEIAWLCLLAGDALLIAGSLLPGI"
     gene            complement(17524..18507)
                     /locus_tag="VFV59_10900"
     CDS             complement(17524..18507)
                     /locus_tag="VFV59_10900"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5326379.1"
                     /translation="MATVSEPETTAAGRARTERWLLLAVAGYAAIVVVMMFAGGLRIT
                     PDVLVVALGLVAVLIGRTKLFLRDWIPFVVIFLAYELMRGIADDAGFPVHVEDVAALE
                     RAIFGGIVPTAWLQERMAPPSGVGAAAIVATVFYMLHFALPVVTGFVLWVWRRPRYHD
                     YLAAFILLSFGGFVTFLLLPVAPPWMAAQRGVLDGPDGQPILHYLKPDAFAAIAEFLG
                     YPDGHELSSYVFYGVNPNGVAAFPSLHAGYPFLSFLVLRQAFGRIGWLALLYAIGVWW
                     SIVFTGDHYVVDVLGGILYAAAAYLAAPRLAHWLAAVVRRLGGRSADPTPA"
     gene            complement(18539..>18901)
                     /locus_tag="VFV59_10905"
     CDS             complement(18539..>18901)
                     /locus_tag="VFV59_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU5326380.1"
                     /translation="GRLASRGVLTIEEVARRRDRSVVRLAGLAVSVQHPMTAKNFVFI
                     ALEDETGMINVTLRPNVYQAHRALLHRHPLLVIEGRLQVEGSVLNVVATRLRSVDEML
                     DAPAMARDALSRQQRMFR"
BASE COUNT         2622 a         6813 c         6777 g         2689 t
ORIGIN      
        1 ccgatgctgt cccagaagct gaaccactcg ccgggataga ggttgcggcc atcgatgtcg
       61 cgcgccggga tgttgatgtt gttgccccac ccgttgccgt ccccggggac gtagtacgtg
      121 gtccacgacg acaccatctg catccgcggc agcacggcct cggcctgtgc ggtcgtgaac
      181 tgcggctcgg tgacgtcgac gacgagcggc accgagcgga cctcctcgcc gtcggcccgc
      241 cgcgtgaagg cgtcgacgag cgatgcgacg ctcgcgtcga cgtccagcgc gcgacccggc
      301 gcgcccggaa cgatgccgcc gaggccgccg cccgaggcga cggtgatccg cgcgttcgtt
      361 gcggcctggt cgatctgctc gaccaggccg ctgaccaggt cggtcagcgc ggccgtgtcg
      421 acggcgacct gcaccgtctc cgcgtccgtc gtgacgtcga gcaggcctgc cagcgtttcg
      481 ctcgaccagg cgatgggcgc ctcgtcctcg gccgcgcccg gaatggtcag atcgagcggg
      541 cgaacactcg cggtcgcgtc gaccgccgcg gcgcgcgcct catcggtggt gatcgccggg
      601 gccgtggtcg tcgccgacag ctggatcgcg acgtcgcccg gccgcgtcgt gtcggcggcg
      661 cgcgccaggg ctgccttgac cgcctcggca tcgacgacct cgccgtcgcg cgacggcgtg
      721 aaggtgaagc gcgcgccgtc ctggacgagc gcggcgtcca ccggcgcgac gctcagctcg
      781 tccgcgacat cgcccgcggc cgcgtcgagc gcctcgggat cgtacgggtg gacggcgacc
      841 gggagtgctg cgcggtgcac gacggaccgc agacgctcga ggcccgccag gaaggggttg
      901 tcgtctcggc ccacatcgaa cgccgcggcc gccatccggt ccaggtcgta gccgcggccg
      961 aggtcggcga acgccacgga cgcctcgcgg tcgccgaccg tgacccggac cgtcccgcga
     1021 tccagtgctg gcagctcgtc cgccaggcgc gcgcgcgcat cgtcctgggt gagcccagcg
     1081 aggtcgacgc cggcgacgct gacgccgggc atcacggcgc cggcgttggc cgccccgatg
     1141 gcgacggcgg tgccggcgag catggcgaga ccgagcagca gcgtgcccgc gaagccgatg
     1201 agcatggagc ggcgcgcggg cggcgagccc atcgggcgca gaaggcgccg gggcatggtg
     1261 atcgtcgtca tggtgtctgg caggagacgc tctcgtatcg ccattcgttg cgatccgccg
     1321 ggcagcggcc gcatcgggcg ttttgctcgg tctccgccgt ggttcctccc tgggctcggt
     1381 tcgccgccgt gcgccgggcc tgggcgcgtt cggcgagatg ccgattgtcg cgggtcagcg
     1441 ccgatctgtc gagtggctcg atcggggcgg gactgctata ctcccggctc cgcacggcac
     1501 tccggagagt gctgttgttg tgtgccgatc acactcacct gaggacacca cgtggcaaga
     1561 acgcctcgat cctggaaggg cgcgcgcacg ccctccgccc tcaagcgcgt gcgccagtcg
     1621 cttcgccgcc gcgctgtgaa ccgtcgcacg cgcagcgagg cgaagacgct cgtccagaac
     1681 gcggcggcga tcgccctcgg ccgcgtcgac ggcgacggcc cggaggccgt gactgcggcc
     1741 atcagcgcgc tcgacaaggc cgccgagaag ggcatcatcc acccgaacaa cgccgcgcgc
     1801 cgcaagagcc gcctgatggc caaggtcaac gccgcgcacc tgctcgacga ggcgcagacg
     1861 gccgccggtg ccgcgccgaa gaagcgcacg gccgccagca agacggcgca gcgcaagaag
     1921 gtccaggccg ccaaggcgtc caaggccgcc gcgggcaagg ccgcggaccg gccgcgcacc
     1981 gccgccggca aggcgaaggt ggccgtgacg cgggccagcc gcgagtccgc cgcggcccga
     2041 cgtcgcaagc aggccggcga gggatccgac gagagctgag ctcacccacg cacaggcaac
     2101 gaccccggat ccgagcgatc cggggtcgtt ctggttccgg ggtgctgatc gccgcgttca
     2161 gcggcgctga cggaaggctc cgcggccggc gaagacggcc gcatcggcca gctcctcctc
     2221 gattcgcagc agccggttgt acttggcgat ccgctcggat cggctgatgg agccggtctt
     2281 gatctgaccg gtgttgaggg caaccgccag gtcggcgatg gtggtgtcct cggtctcgcc
     2341 cgagcggtgg ctgatgaccg cggtccagcc ggcccgctgc gccatctcga tggcctcgat
     2401 cgtctcggtc accgtgccga tctggttgag cttgaccagg atgctgttcg cggcgcgctg
     2461 ctcgattccg cgcgccaggc gctcggtgtt cgtcaccagc aggtcgtccc cgacgatctg
     2521 gatgcgctcg ccgacgcgct cggtcagcgc gcgccagccg tcccagtcgt cttcggcaag
     2581 gccgtcctcg aggctcacga tcgggtagcg ctcgacccat cctgcccaga actcgaccag
     2641 ctcctcgctg gtcatgctcc ggtcctcgcg agccagcgtg tagtggccgt cgccgtacag
     2701 ctcggtggtc gctggatcga gcgcgatggc gatctgctcg cccggcgtgt atccggcgcg
     2761 ctcgatggcc gccaggacgg cggcgatggc gtcctcgttg ctgccgaggc tcggggcata
     2821 gccgccctcg tcccccaccg tggtggcgaa gccctcgtcg tgaaggatct tgcccagggc
     2881 gtggaacgtc tccgccgccc agcgcagcgc ctcccggaag ctcggcgcgc cgagtggcgc
     2941 gatcatgaac tcctggaagt ccgtcgagtt gatggcgtgc ttgccgccgt tgaggatgtt
     3001 cacgagcggg accggcagcg tccgcgcccc tgccccaccc aggtatcggt acagcggcag
     3061 cccgaccgac tccgcggcgg cgtgggccac cgccatgctc acgccgagca gcgcgttggc
     3121 gcccagcttc cccttgttgt ccgtgccgtc gagcgcgatc agcagccggt cgacgctcac
     3181 ctggtccacc gcatcgacac cgatcagctc gggacgcagg gtgtcgttga cgttcttgac
     3241 agcggtcagg gtgcccttgc cgccgtaccg gtccccgtcg ccgtcgcgca gctcgaccgc
     3301 ctcgtgggcg ccggtggacg cgccggacgg cacgatggcc gtcccgaccg agccgtcctc
     3361 gagcagcacg tcgacctcga cggtcgggtt gccgcgggaa tcgaggacct cccgtccgat
     3421 gacgtcctcg atcagcgagc tggtcatgcc ggcacctccg cctcgcgccg cagcagcgcg
     3481 gccacgccgg gcagcgtctt gccctcgagg aactcgagcg acgcgccgcc gcccgtgctc
     3541 acgtgggtca tccggtcggt cagctccagc tgctccacgg ccgccaccga gtcgccaccg
     3601 ccgaccacgg tcacggcgcc ctggtcggtg agcgtcgcca ggctgcgcgc gatggccctg
     3661 gtgccctccg cgaactgcgg gtgctcgaag acgcccatcg gtccgttcca gaagacggtc
     3721 ttcgccttcg ccaggtgctc ggtgtacgcg gcgaccgtct cggggccgat gtccacgatc
     3781 atctggtcct tgggcaccgc gtccaccggg accaccttcg tcttggcgcg gtggtggatt
     3841 tgcggcgcga tgaccgcatc ggtcggaagc atcaggcgga cgcgcttgcg gcgcgcctcc
     3901 tcgacgatcc gcgtggcgtt gtcgagctgc tcctcctcca ccaggctctt gccgagggcc
     3961 aggcccttgg acgccaggaa ggtgttggcc atgccgccgc cgaccaggat cgcctggcag
     4021 cgcgacagca gcgcctcgag cacctcgagc ttgccggaga ccttggcccc gccgatgacg
     4081 gcgtggaacg gcttggccgg tcgctcgagc agcgtctgga gcgcatcgac ctcgcgctcc
     4141 atcaggaggc ccgccacgga cggcaggtag tgggtgatgc cctcggtcga ggcgtgcgcc
     4201 cggtgggcgg cgccgaaggc gtcgttgacg tacaggtcgg ccaggccggc gagcgacttc
     4261 gcgaactccg ggtcgttggc ctcctcctcg gcgtggaacc ggaggttctc gagcaggatc
     4321 atgtcgccgg gccgcagctt gtcgaccgcg acctgcaccc ccggccccag cgcatcgccg
     4381 gtcatgcgca ccgggcgccc gagcagctgg ctgagccgat ccgcgaccgg cttgaggcgc
     4441 agcgcgtcgt tcaccttgcc cttcggccgg ccgaggtggc tcgccaggat gaccgtggcg
     4501 ccgcggtcca ggagatggac gatggtcggc aggctggccc ggatgcgcgt gtcatcggtg
     4561 atcctgccgt catcgagcgg gacgttcagg tcggcgcgca ggaagacgcg cttcccgtcg
     4621 acgtcgacgt cccgaacggt cttgtagtcc atcggtgcat tcgcccccgt ctagagccgc
     4681 tcggccatga acgcggcgag gtccgccacg cggcacgagt agccccactc gttgtcgtac
     4741 caggcgatca ccttcagcag ccggtcgccg gtgaccatgg tcgaggccgc gtcgatgatg
     4801 ctcgagcgcg agtcgccctt gaagtccatg ctgaccagct cctcggtggt gtagccgagc
     4861 aggccctgca tcggcccttc cgccgccgcg cgcagcgcat cgttggccga ttcggccgtc
     4921 accggcttct cggtgatggc cacgaagtcg atgacgctca cggtgggagt cggcacgcgc
     4981 agcgagaagc cgtcgaactt gcccttgagc tccgggatga ccagggccag ggccttcgcc
     5041 gcgcccgtcg tggtcgggat gatgttctgg ccggcgctgc gggcgcgccg caggtccttg
     5101 tggaagacgt cgaggacgtt ctggtcgttg gtgtagctgt ggatggtcgt catcaggcca
     5161 cgctcgatgc cgaacgtgtc gaagacgacc ttcgccggca gcgccaggcc gttcgtcgtg
     5221 cagcttgcgt tgctgacgat gtggtgctgc tccgggtcgt acttgtcctc gttcacgccg
     5281 agcacgaggg tcacgtcctc gttcttggcc ggcgccgaga tgatcacctt ccgggcaccg
     5341 gcctcgacgt gggcccgggc cttggtcgca tcggtgaaga tgccggtcga ctcgatgacg
     5401 atgtcgacct ggaggtcctt ccacggcagc gccgccgggt ccttctcgct gaaggcgcgg
     5461 atctcgtggc cgtcgaccgt gatggtgttc tcgccggcgg tgacgtcgcc ctcgaacgtc
     5521 ccgtacgtcg agtcgtactt gaacaggtgc gcgttcgtcc tggtcggcgc caggtcgttg
     5581 acggcaacca cctcgagctc gtcgggatgg cgctccagga tggcgcgcag gctctggcga
     5641 ccgatgcggc cgaagccgtt gataccgatg cgggtcgtca tgagggtgaa gctccttgct
     5701 gtgaggccgc cgatggcggc cggctcgccg ccgcccggaa ggcggcgtcg tggatacggt
     5761 cattgacgat cggcaggagt ccgatcagtg agacgtcgcc acccagcgcg gcgcgctcga
     5821 tgcgcacgcg gtggcggagg aaggcgaagg cgcgacgctc gagctcggcg tcgatgacct
     5881 cgaagagccg tggttggtgc tgggcgatcg atccgcccag gacgatcacc tcgggatcca
     5941 ggacgttgac cagcgacgcg cacagcgcac cgatggcctc ccacgcgcgc tccaggacct
     6001 gtcccgcgtc gtcatcgccc gcgtgggcgg ccgccgcgag cagctccgcg tcgggctcgc
     6061 gaccatcggc ggccaggcgg gcaagcacgg gagcgcgtcc cgcgtcgagc agggcccgcg
     6121 cctctcgcgc gagggccgtg ccggacccga tggcctcggc gtggccgacg ccaccgcagc
     6181 cgcagatcgg gccgtcgagg ttgaccgtca tgtgcccgac ctcgccggcc gaaccgtcgg
     6241 ggccgatgat cggacgcccg tccagcacga tcccgccgcc gatgcccgtc gagacggtga
     6301 tgtagatgac gttctgcgcc ccacgcccgg cgccgtagcg ccactcggcc atgacggcaa
     6361 cgttggtgtc gcgctcgagg taggtggcca ggccgatgcg cggggcgacg tgatcgacga
     6421 cggacacgtt ctcccacccg tgcaggttcg gggccgccag cacgatgccg cgccagcggt
     6481 ccagcggacc cggcgccgag atgccgaggc ccaccggctc ggggtcgccg gcacggcgcg
     6541 cgtcgtcacg gaccgcggcg gcgatctcca cgatccggtc gagcaccgcg gtcacgccgt
     6601 cctcgtcgcg cgtcttgacg gcgcggcggt ggcggacctc ccggtccggg gtgatcagtg
     6661 ctgcgcggat ctgggtgccg ccgaggtcga ccgcgaggac gggtcgttcg gctgcgagct
     6721 ccacatccct gattgtaggg aggccgtcgc ggaacgaccg gtgaaggtgg cccccagatg
     6781 cgtgacgaac ggcacccgga acgcgcgggg caaaacgaag gacgcgccgg cgggtgccgg
     6841 cgcgtcctgt cgaacggtgt tcgacgttcg ggtggcgggt cagccgccga tcttgaacat
     6901 cttggtgccg cagaccgggc acacgccctg ggtcgccggc ttgccgttct tcatcgtgat
     6961 cttctcagcg ttctgcatct cgcgcttggt cttgcacttg acgcagtagg cctcagtcgc
     7021 catcggttcg ttgcctccct gttaggaccg tcactcggtt gcggtcggtg cctgtgagct
     7081 tccttcctgc ccggtgcccg acggtcttcg gctggcgagc gtgtcgccag catcggtgtc
     7141 ggcctgccac agcaggtgga tgccggccag tccggcatcg atgcagcgtg cctccgcggc
     7201 ctccttcgcc gcgccttcga ggaacgtgcc gtccgtccgc tgcgcgtagg ccgcgcagac
     7261 cgtgccggac cgcagtcccg caagatgcgc cagcacgaac agtgccgacg attccatctc
     7321 gaggttcgcc acacgctggc ggcgaagctc atctgcgagc gcaggatagc gaaccgggag
     7381 cgtgcgcatc gcacgtccct gcggagcgta gaacccgctt gccgtggccg tgagccccac
     7441 gtggtaccgc acgccctggt ctcggcacgc ctgctccagg gcccagacca cgtcgcgatg
     7501 cgcgatcgcc gggaagccgg gatggacgta gaagtccgtc gtgttctcca gtcgcacggc
     7561 cccggtcgac acgatcaggt cgccgagggc gatctcgggc tggagggcgc cggatgaacc
     7621 gatgcgaatg agcgttggcc gatccgtgat ctccatcacc tcggccagga cgatctccac
     7681 gttgtcggta cccatgcccg tcgacatgca cgacacgcgc atgtcgcgat acgtcccggt
     7741 tgcggtggtg atctcgcggt tgctgcgctc gacctccacg ccatcccagc gctcggcaac
     7801 cttcgcgacg cgacccggat cgccgaccag caggatgtac tccgccagct ccccccgctc
     7861 gagggcgatg tggtactggc gggtcccggt catcggtgcg tccgcctggg tcaccccggt
     7921 cacgtcgcgg cgtctcctcc tgctcgtccg gcgtcgaacg ggcgggagta tacgctcggc
     7981 ttcccctcat ggaccacgct cgcgcggcac ggcgacgagc gtgagggccg gcaccctgac
     8041 cgccggctcc aactccgtgg cgtcctcgtc gtcgtcgtcg tgggtggtgt cggcgtcggt
     8101 agtgctcagc tcagggccct cacccccgag ccgaagcgcc cgcgcgatgg ccgccgccac
     8161 caccaggaac aggccgacca ggaggacgag cacggccgcc aggcgagagt cgtccgcccc
     8221 cgagccggac gcatcgggtg ctgtcgacgt gctgaggacg gccacgtccg agggacccgg
     8281 cagggcgagg gacgggccgg ctcgcagtac cggcgcggtg cggatgccgt cgcagccgac
     8341 ggtgatcgct gccggggacg cgctgccgat gccggtcacg ctcacctggc cgcggtcgcc
     8401 cgcatgcagc gtgcgggcgg cgcacgcgtt gacgacggtg accgggccac ggcccgcgag
     8461 catgaccgcc gcgcggtcgt ccgtggcacc gcggatcagg cgaccgacga cagtgaccca
     8521 gcgcggggcc tcgcccgcgc ccggcagctg cgtcaccgga gcggcgagtg ccccgccgct
     8581 cgccacgtgg ccgctgccga gggtgacgat cgcgatgccg gtcgcctcgt gccggccggc
     8641 gccgctcagc ccggtgagcc gcagcggcag cggcaccgtg acacgtggca gcgatcgcgc
     8701 gatcgcggcg gccgcctgat cgtcgatggc gaccagccgt tcgccgtccc agagaaggcc
     8761 cgcgacgccc agctccggcc atcgcgcggc caccagggtc gcgccgatct cgaggtcgtg
     8821 cggggcagcg gcccggtcga gggacctgag gctgacggtt ccgccgcggc tggttgcgtc
     8881 ccgatccgca ccgcccccgc cacggccatc gccgttggag cccgatgcgc cgtcggcacc
     8941 gtcagcatcg ccggcggacg cggtcacgcg aaggtcgctc gggtcgcgcg gccagagccg
     9001 gtagccgcgc agcggttgtg cgccggtcgt ctcctgcccc acgaccccga ccacctcgat
     9061 ccagtcgccc gcgttcagct cggcgtcgac gccgacggcc gaggtggcga cgacccgcaa
     9121 cggcccagtg ccgtcatcca cgtcgaagct gaccgttccg ttcgacgagt gccgtggcga
     9181 cgacaccgcg ccgcgcacca gcaccagctg cgcctccagc ggctctccga cgtcgcccgt
     9241 ccgcacggcc cgcggcgccg gctcagtggc cggacccagg tcggacggcg gcaccgtgac
     9301 gcgcaccgtc tccatgccgc ccttggtcga ccgcgccccg ctcacatcga ccaggcggcc
     9361 acgagtcagg gctcccgcgt cgtcaccgag ccgcagcacg atggcgccgg tcgcgtcctg
     9421 cacgacggcg gtgctgtcgt cgacaaggcc gctggggagc gtcacgacgc cgcgaaccct
     9481 gacggcggcg ttcttcgacg cctggcgtgc ctcggcgatc gacatggtcc cgggggcggg
     9541 cgttggaacc gccgagcccc cgggcgtcgc cgtcgccccg gacgtgggcg agggcgaggg
     9601 cgtggggatc gggctcggcg cgggcgtcac cgagaccacg tcggccgggt cacgtggctg
     9661 gagtcgatac ccggccgcgc ccgtcccgct cgagtcgcgc tgcccgacca cgccgaccag
     9721 gtcgagcgac gtcccgacgc tccacgtcgt cgtgtcgatg ccggtcgacg tggcgacgag
     9781 cacccgcgcg acgccggtcc cgtcgtcgac gtcgtacgcc ggaccgctgc tcagcatcgt
     9841 cggaccgtcg acgacggtgg cggtcacgcg gaccagggat ccctcgaacg cctcgccgat
     9901 ctgcccgctg gcgacggcgg tcgccggcgg ggcgtcgccg gtgccgagcg tgaccacgtc
     9961 gagcgcatcg gcgcggatgg tccgctgggc gtagcggtcg tccaggctgc ccgtgactcg
    10021 cacatgcgca ccacgcgaga tcgcgccgcc gctgaccagc accgcgatgc cggcgctcgc
    10081 atcggccacg tatccgcctc cgtcggagaa cgccgagtcg gtgagcgcca cgccctcgat
    10141 ggtgacgctg gtgccgtccg gcagcccgcg cgcgaccgcg acgtcgacga ccggcgcggg
    10201 cgagggactg ggcgacccgc tcggggtggg cgtggacgtg ggcgtgggcg tcggaggtgg
    10261 cgttgacgtc ggcgtggggt ccggtgtcgg ggacgttgtc gcggaaggcg tcccgctcgg
    10321 ctccggcgtc ggctccgcgg tcggcgtcga cgacggtgtg gccgtggggg acccaccggg
    10381 cgcgggcgtc ggcagcgatg cgcggttctg gggatcgggc accgtcagca gcgcgaagtc
    10441 ggtggcgttg tcgtcggtgt cctgcgtgga gcccagcgca ccaccgggca ggcgctcgat
    10501 gctcgatccc gccgccggcg ctggcgccgg caccccctcg agccaagtcc cggtggcggt
    10561 accccagccg acggcgtcga tggcggtcgt cgcgccctgg atgcgcagcg cgacgctgcc
    10621 ccccgtcgcg gccatcccgg tggcgtaggt cacatcggcg atcgaggcgt agaccccggc
    10681 ctcgttcgcg atgagcagat gcgcctgcgg ctcgacgggc ggtgccccga gcgcccacgc
    10741 cgcgcgacgg ctcaccgtcg ccccgctggc gctgacgtag accagctcga gcccttccag
    10801 gggcaacggc gtcgcggtcg ggttgtagat ctcgatcagc tcgtcgctgg cgctcgcccc
    10861 gccggtgacg acctcgctga tgaggaggtg gtcgggcgcc gcggcgacgg cggtgagggt
    10921 cgccagcggc agggtcgcga cgacgagcac ggaaacgagc agtccgacga agcccgggga
    10981 gcgggcggcc ggcaccgtgg ccatggagaa acgccgagcg gacacgaagc acctcgcagc
    11041 tcggaggcgc cgcaggcgga gcggaggatg ctccccggcc gcttaccggc cggttagtgc
    11101 acgcttaccg gtcggcatcg gtacaatcgc gcccgatgtc caaccgcacc cccgacgacc
    11161 gcgcggcatc ccgcgccgag gcacgacgtc gcgctcgcct ggccgcgcgc ggcgaactca
    11221 gcgaaaccga agcatcggcc gaggagccgg ctcccgccgc gtccggctcg ggcggcggct
    11281 tcctgagccg catcttcccg ccggccccgg cgctgcccgg tcggcccgac ccgctggccg
    11341 gcttcgaccg cagcgggccg ctgcgtccgg ttcgcgaacg tctcttcctg ctgcgctcga
    11401 acctggtggc ctggctggtg accggcctcg tcgccttcat cggcttctac gcgtcgttcg
    11461 cgtacgccgg cagcatcctg gggatcgtcg gcaccttcgt cctgttcggc gcgttgatcg
    11521 gcgcggggtg gttcggctgg cagcgtccca cgctgttcgg cacggccgcc gccctcgtga
    11581 gtttcgtgat cggcgcggca cttctgatcg tcgccttcag cgccgccgac tccaccatcc
    11641 agaccgccga catcgtggcg tcgctcgtcc cgcaggccct ctaccaggcg gtgctcggct
    11701 tcatcggcgg ctggtatggc ggctacctcc gacgccggca gacgcagatc agccagcaga
    11761 cgcgcccgtc ccgacgccgc tgatccacgc ggaaccgccg gcgccgcggc acggttcttg
    11821 ctcctctggc gcgacgtaga catcgagcct tccgggtcat ccccggacga acgaagcctc
    11881 gttgcgaacg gaagctcggg ccgaacgcga ccttgtgcgg cttcgctcgt tcgatgacca
    11941 ggttcgtgtc ttccgggccg tcggttccag gaccctccgt actggagccg acggccctcc
    12001 ttcgtgcctg ggaccggtcg ctcaggacgc gtgagccgat gcggcctcga gcgccagccg
    12061 atgtgcatcg gcggcgaccg gcgcgagccc cagcacttcg ccggtggagc gcatctccgg
    12121 gccgagatct cggctgaccc ccggcagccg ggccagcgac ccgaccggag ccttgaccgc
    12181 gacgagccga ccgtcggcgt gcagccccgg cgacaacccg acggacccca ggtcggcgcc
    12241 cagggcgcag cgcaccgcgg ccgccaccac gtcgagcccg gtggccttgg cgacgatcgg
    12301 caccgtgcgg ctggcgcgcg ggttggcctc gatgacgacg acgcgctcgc ccacgacgac
    12361 catctgcacg ttgaggatgc cgcgaacccc gagcgccagc gcgatccgtc cggcggcttc
    12421 ggccgctcgc tcctgctgct cgcgcggcag tcgctgtggt ggcagcaccg cgacgctgtc
    12481 gcctgagtgc acgccgggtg ggtcgacctg ctcgaggatg cccggcaccg accacgcctc
    12541 gccatcgctg atggcatcca cgtccagctc gatgccctcg agcatctcgt ccacgcgcag
    12601 cggccagccg acgtcggtgg ccaggtactc gcgaacgtcc tggtccgtgc ggaggaccgc
    12661 gatcccccgt cctccgatca cccacgacgg ccgcacgatc accggcaggc cgccgagcgc
    12721 gccgatcgcc tcgcgcaggg agccctcgtc ggacgcaagg gacccgcgcg gaccggcgag
    12781 gccgagctgg ttcagcagct gcgcgaagcg ctcgcggtcc tcggtgatct cgatcgcctc
    12841 ggccgtcagc ccggcgatcg gcacgtgggc gtacgccagg tccttggcca gatcgatggc
    12901 cgtctggccg ccgaaggtca gcaccacggc cgggggcgtg ccggtcaggg cccgctcgtg
    12961 atcgatcacg tccaggaccg actcggtgtc gagtggctcg aagtagagcc gcgtgctggc
    13021 gtcgtaatcg gtgctgacgg tctcggggtt gttgttgaca acgacggcat cgagccccat
    13081 gtcccggatg gcccacgccg cccgcacgct gcagtagtcg aactcgatcc cctgcccgat
    13141 ccggatcggt cccgacccca ccacgatgac gctctgccgc tcggccggcg gcgccacctc
    13201 gggctcggcg tagctcgagt agaagtacgg cgtctccgcc ggaaactcgc cggcgcaggt
    13261 atcgacccgc cggtaggccg gaccgatgcc ggcgcgcacc cgagcgcgcc ggatggcgac
    13321 tgccgggacg ttcgagagct gcccgatgtc cgcgtcgccg aatcccgtgc gcttcgcgtc
    13381 gacgagtggg gcgcccacga ccgtggcttc ggcggcgacg agctccgcga tgcgctccgt
    13441 gaaccacggc gcaacaccgg tggccgccga gatgtcggcg gcatcggcca aaccatggcg
    13501 cagcagggcc accatccgcc acagccgcgt gtcggatggc gcgaggaagg cctcgaggtc
    13561 gtccggggcg cgttcgtcca gcgcccaggc cgggtcctcc cacagccagc cgcggccgcg
    13621 cggctcgagc gagcgcagcg ccttcagcag ggccggaccg aactcgcggt cgatggccat
    13681 cgcctcaccg gtggccttca tctggatgcc cagggagcga tcggcggtgg ggaacttgtc
    13741 gaacggccag cgcggcagct tgaccacgac gtaatccacg gccggttcga acgccgcgct
    13801 gttgccgccg gtcgccgggt tgggcatctc gtgcagcagc cgccccaggg cgatctgggc
    13861 ggcgatccgg gcgatcgggt agccggtcgc cttggacgcg agggccgacg agcggctcac
    13921 gcgcggattg acctcgatga cccggtagtc ggcatggtcc ggtgagacgg cgagctggac
    13981 gttgcagccg ccctcgagct tgagggcacg aacgatgttc agcgcgcatc ggcgcaggcg
    14041 ctggaccacc gggtcgggca gggtctggat cggcgccacc acgatcgagt cgccggtgtg
    14101 cacccccatc gggtccaggt tctccatgcc gcagatggcg atggtcgtgt cggccgcgtc
    14161 gcgcaggacc tcgaactcga tctcgtacca cccgagcagc gagcgctcga ccagcacctg
    14221 gtggatggga ctggccgcca ggccagccgc gatgcggtcc cgcgcctcat cgggcgtcgc
    14281 cgcgaacccg ccaccacccc caccaagcgt gaacgccggc cgcaccacga ccggcgcgcc
    14341 gagcatctcc acgaagcgga gaccgtcctc gacggactcg atcacggcgg actcgggcac
    14401 cggctcaccg atccgcatca ccaggtcgcg gaacccaccc cgatcctccg cggcgcgcac
    14461 cgcatcgagc ggcgtgccca ggacgcggac gccgtatcgg tcgagcacac cggcgtcgtc
    14521 cagggcgacg gccaggttga ggcccgtctg tccgcccagc gtcggcagca gcgcgtcagg
    14581 ccggtgctgc tcgatcaccc tggtgacgtg gtcgagcgtc agcgggccga tgatcacggt
    14641 gccgccgacg tccggatcgg tcatcaccgt ggcggggttc gagttgacca ggaccgtctc
    14701 cacgccggcc gcccgcagcg ccaggcaggc ctgcacgccc gcatagtcga actcggccgc
    14761 ctggccgatg aggatcggcc cggagccgat gaccaagacc ttgcgcggcg gcctcgtggt
    14821 ggtcgtcata ccgtcgcccc gctcgtggcg aggtcgaaga cgcgtgccgc gtcgatgggg
    14881 cctggcgcgc cctcggggtg gaactgcacc gatgcgatgc ggccgctggc gtggaccatt
    14941 ccctcgacgg tgtcgtcgtt caggtcgcgg tggctgatgc cgtacccggc ggcggcgagg
    15001 gccggcccgt ccagcaccgc gacctcgtgg ttgtgcgccc cgatgtccac gcgaccggcg
    15061 gccacgtcca ggaccgcgtg gttgccgccg tggtgtccga ccggcatgcg gtgcgtctcc
    15121 gcgccggcgg cgagggccag cagctggtgg cccagacaga ttccgagaat gggcgtcccg
    15181 ccatctgccg cggccgccgc cagcgtccgc accgtcgcga tctggggcgc catgcggctc
    15241 ggatcacccg gcccgggcga gaggacgacg aggtcggggc gcgtggtgcg aacgtccgcg
    15301 gcggtggcgt cgggcggcag gaccgtgacc accagcccgc gctgcgtcag ggcccgcagc
    15361 agcgagcgct tgacgccgta atccaccagc gtcgcacggc cgcggacctc gccctgcggc
    15421 gccacgacgt acggccgcga cgtcgccacc acgccaacgt ggtcgacgga gtcccagggc
    15481 gcgctgtcac gcgcaccttc cacggcgtcg tcggctgcca ccgcacggtc gacgatgagc
    15541 gcgcggcgag cggcaccgtt gcggagccgc agcgtcagcg cccgggtgtc gacgcccgag
    15601 atggccggga tgccaccctc ggtcaggagc tccgagaggg aggcgcccgc accgggcgga
    15661 ggctcgacga ggcgggtcac gaccagcgcg cgggcacgaa cgtggtcgga ctccagctcg
    15721 gagcgatcga tgcggtagtt ccccgccatc gggtgggtca ggacgacgat ctgcccggcg
    15781 taggacggat cggtcaggat ctccccccat cccgtcgagg cggtggtgaa gacgagctcg
    15841 ccgctggcgg gcgctggtcc accgtgcagc cgaccctcga gcaccagccc accctccagc
    15901 gccagtcggc cggggcgagg ctcggaggaa gacgacgacg ccacccgcgg tgctgcggac
    15961 ggcgtcgaat cggttgcgag gtcgagatga tgtgcggtct gcacgcgcgc tcctttccgg
    16021 cctcactggg ccgggcttaa aggattcgcg cgccagtgta cccgatcagg cgggcgcgtc
    16081 gtcgcggcgc ggagccggca tgcggcgccg gacctccagg acgccgcgca gggcgatgac
    16141 gaccagcagg gccaggatga gcaggatcgg cagccacacg atcccgaacc agatcaggcc
    16201 gttgagcagc ccctgaccga tgccaaccag cgccgccagc gccgcattca gctgcgcccc
    16261 gggatcccac gtctcggcct gctggctgat cggctcggcc ttggggacca gggtgatcgt
    16321 gagcgtcgac aggtctgcct ggccctcgag cgccttgagt tgagccgtca gctgctcgat
    16381 ctggccgcgg acctcgtcga ggcgcgattg cacggccagg atgtcctcga tcttctcggc
    16441 ccgcgccacg agctcgcggt agctggcctc ggacgcctcc aggttgtcga tccgtgcgcc
    16501 gaggtcgacg atctgggtcg tgacgttctc ctcgcgcgtc gcctcggcaa tcacttcgcc
    16561 gtcaagctcg tgtagccggg cgagcgcgtc gtcgaacgag gcagccggga tgcgggccgt
    16621 gatcgtcgcg ctgtcgtcga gcgtgccggc ctgcgagccg ccgacgtagc cgctcagctg
    16681 ggtgacgagc gctcgcacgc gcccgagtgt ctcggcgacg ctggccacct cgagggtcac
    16741 ctcgccggtc ttgacgatgc gctgctcgat ggggaccgcg tacgatcccg agccgtcgtc
    16801 ggcgccgcca cctgcgacgc cgcgatcctc gtcgggtgcc gcctcgccgt ccgctccgcc
    16861 agcggcggcg ctggcaccgg ggacgaccgg ctggccgaca tcgccgaagt ccgcgccgcc
    16921 ctgcgcaccg caggcgacga gcagcgccgc gacggccagc agcgcgaaga gtcgtctggt
    16981 catggttcca acctccatcc gtgggcgtga acgggacgcc cgggaggccg gatgggttcc
    17041 gacgccggat cagcgccggc gatgccctcg gctcagaggc cctcgaccac catcccgatg
    17101 atcagcgcgg cagcggcgag caggaaggcc cacacgccga cggcgtcggg cagcacgctg
    17161 ccaaaggtcc cgagcagccc gaccaggacg agggcgagcg cgacgatgat ggtcggcagg
    17221 cgcggggcga aattcatgcg gctcatgggt cagatcccgg gcagcagcga gccggcgatc
    17281 aggagtgcgt caccggcgag gaggcagagc caggcgatct cgctggtcac ctcgatattc
    17341 gtcccgacct gcgcctggac catgcccagc gcctcgttga cgatgtcgat cgggaagatg
    17401 gtggccgaga tgccgaccaa cagcaggaag acggcgatgc cgacggtgat gagccgcggc
    17461 ggattcggat gcagcacggc cgggagtata cggagtgacc gatgccgccg agggctccgc
    17521 gcgtcaggcg ggagtcgggt ccgccgaccg cccacccagt cggcgcacga ccgccgcgag
    17581 ccaatgcgcc agccgcggag cagcgaggta cgccgcggcg gcgtagagga tgccgccgag
    17641 gacatcgacg acgtagtggt cgccggtgaa gacgatcgac caccagacgc cgatggcgta
    17701 gaggagcgcc agccagccga tgcgaccgaa ggcctggcgc agcaccagga agctgaggaa
    17761 cgggtagccg gcgtggagcg acgggaaggc cgcgacgccg ttgggattga cgccgtagaa
    17821 cacgtacgag ctgagctcgt ggccgtcggg gtagccgagg aactcggcga tggccgcgaa
    17881 ggcatcgggc ttgaggtagt gcaggatcgg ctggccgtcg ggaccgtcca gcacgccgcg
    17941 ctgggccgcc atccacggtg gcgcgacggg gagcagcagg aacgtcacga agccgccgaa
    18001 gctcagcagg atgaacgccg ccaggtagtc gtggtagcgc ggccgccgcc acacccacag
    18061 cacgaagccg gtcaccaccg gcagggcgaa gtggagcatg tagaagaccg tcgccacgat
    18121 ggccgccgcg cccacgccgc tgggcggcgc catccgctcc tgcagccagg cggtcgggac
    18181 gatcccgccg aagatggcgc gctcgagcgc ggcgacgtcc tcgacgtgca ccgggaatcc
    18241 cgcgtcgtcg gcgatgccgc gcatcagctc gtacgccagg aagatgacca cgaacgggat
    18301 ccagtcgcgc aggaacagct tcgtgcgacc gatgagcacg gcgaccagcc cgagcgccac
    18361 caccagcacg tccggcgtga tgcgcaggcc gccggcgaac atcatgacca cgacgatcgc
    18421 cgcgtagccg gcgaccgcca gcagcagcca acgctcggtt cgcgcgcggc cggctgccgt
    18481 ggtctcgggt tcgctcacgg tggccatcgc ggccgatggt accgagccga gggctgggtc
    18541 agcggaacat gcgctgttgc cgcgagagcg cgtccctggc catggccgga gcgtcgagca
    18601 tctcgtccac gctgcggagc cgcgtggcga cgacgttcag cacgctgccc tcgacctgca
    18661 gccgtccctc gatcacgagc agtgggtggc ggtgcagcag cgcccggtgc gcctggtaca
    18721 cgttgggccg cagcgtgacg ttgatcattc ccgtctcgtc ctcgagcgcg atgaagacga
    18781 agttcttggc cgtcatgggg tgctggacgc tgaccgcgag gcccgccagg cgcaccacgg
    18841 accgatcgcg acgccgtgcc acctcctcga tggtgagcac cccgcgcgac gccagccggc
    18901 c
//
LOCUS       DASTYP010000251         4627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_689314, whole genome shotgun sequence.
ACCESSION   DASTYP010000251 DASTYP010000000
VERSION     DASTYP010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689314"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFV59_10910"
     CDS             complement(<1..195)
                     /locus_tag="VFV59_10910"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5326381.1"
                     /translation="MKVIGLTGNIGCGKSTVAGMLREHGVATIDADVVAREIRHNDAD
                     ARRAIEARFGTYEAAELGRIV"
     gene            complement(192..1106)
                     /gene="mutM"
                     /locus_tag="VFV59_10915"
     CDS             complement(192..1106)
                     /gene="mutM"
                     /locus_tag="VFV59_10915"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU5326382.1"
                     /translation="MPELPEVETVARDLQQWVAGATIREATVTWDRTIRHPQPPERFV
                     AEIAGATIQRVTRRAKTVLIHLTDGRVMTVALRMTGALIVTPPGAPPDPYARVVFELS
                     DGRELRYRDVRKFGRIGLWPGGGLRSVGGGRGARSRKVKEGSRRYRIGEVFSGHGPEP
                     LSHGFTARRFAERLARRSARLKTLLLDQSFIAGVGNIYADEALWRARLHPLRAADTLT
                     EAEVRRLHRTVRQVLREGIANRGASFSDYVDARGEEGTNQERLAVYRRTDEPCYRCGR
                     PIRRIVVGQRSTHFCPRCQAESVASAAE"
     gene            complement(1221..4097)
                     /gene="polA"
                     /locus_tag="VFV59_10920"
     CDS             complement(1221..4097)
                     /gene="polA"
                     /locus_tag="VFV59_10920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5326383.1"
                     /translation="MSERRPLLMLIDGPSLVYRGYFALPPLTLSDGTLVNAVFGFLQI
                     VLRGMQDVKPDYAMLSFDVGKPQFRFDAHPEYKAGRPSMPDDLRAQFPIVREIAQMMG
                     MPIRELQGYEADDVIGTLSKKATAQGVDTMIVSGDLDGLQLVDDHVRLLTTRMGVAAT
                     VIYDEAKVMERYGLRPDQMLDYKALKGDTSDNIPGVPGVGEKTAISLLQQFGTLDGIY
                     ERLDEVKGKLRDRLAEHRASAFMSREIGRIVCDLPVELDLEEARTGRYDRRAVAQRMR
                     ELEFRSLIDRLPPSTIAPTGYDGPEETKGGLQLSLDLLGGAAPSGGPAAAPSDEPRSR
                     PDPLADPAGAIEHVDPRIVRTDADRAELETWMAAHEGGIGIGWSAGAGRPLERPLFGV
                     ALAGGDGSSWYVPWTEDRPLPGWFGREDRDLVGHDLKQLMVMLGSRDVPLAGACFDTL
                     VAGYMVNPALRAQTLDDVAATRFGGQLPPKPLTPETGAEEVGCARRAAGEALTALLSK
                     PALESELADAALTNLFHEVEMPLVPVLARMELAGIEIDLDALAAMQEEFGAQLTGLEA
                     RIYDLVGRPFNIGSPKQLEQILFHELGLPATKRTRTGYSTDASVLEELRDKHEVIGLI
                     LEHRQVSKLKSTYVDALPGLVDANRRLHTTYQQAVAATGRLSSTDPNLQNIPIRTPLG
                     RRIRRAFVAPEGKLLLGADYSQQELRILAHVSADPGLRADFASHSDIHLAAAARVLGL
                     APDQVGPKERSVAKMINYGIAYGLSDFGLAERLKIPREEAQRFIADYFAAYEGIRRYT
                     VEMKILARDQGYVTTLLGRRRYLPELTARNGALRSAGERMAINMPIQGTAADGMKIAM
                     VRVDQALRERDLRSKMLLQVHDELVFETDEDELPELAGLVKEIMESALPLDVPLEVDL
                     KVGRNWEQMDRYLRSDAGEWRRVPKTAVDVAREEADAEVAADLV"
     gene            4152..>4627
                     /locus_tag="VFV59_10925"
     CDS             4152..>4627
                     /locus_tag="VFV59_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU5326384.1"
                     /translation="MHSRPSIDPYAVLGVGREATPLQVARAHRRLAKAHHPDLNEGAS
                     EASERMRRINEAWSILSNPTRRAEYDRSHPTAGRPPGSHWAGTRSPIQPASPTSTRTW
                     ASWRATADQTRAAPRTVRAPGERPVPRTRRPPRPDPMPTTFRDSPWAAILAAALIVG"
BASE COUNT          616 a         1723 c         1597 g          691 t
ORIGIN      
        1 gacgatgcgg cccagctccg cggcctcgta cgtcccgaag cgcgcctcga tcgcgcggcg
       61 cgcatcggcg tcgttatggc ggatctcgcg cgcgaccacg tccgcgtcga tcgttgccac
      121 gccgtgctcg cgcagcatgc cagcgaccgt cgacttcccg cagccgatgt tgccggtcag
      181 cccgatgacc ttcattcggc tgccgatgct accgactcgg cctggcagcg cgggcagaag
      241 tgcgtgctgc gctgcccgac cacgatccgt cggatgggcc gcccgcaccg gtagcatggc
      301 tcgtccgtcc ggcggtagac cgccagccgc tcctggttcg tgccctcctc gccgcgggca
      361 tcgacgtagt cgctgaagct cgccccccgg ttcgcgatgc cctctcgcag cacctgccgc
      421 acggtccgat ggagtcggcg cacctcggcc tccgtcagcg tgtcggcggc tcgcagcggg
      481 tggaggcgtg cgcgccacag cgcctcgtcg gcgtagatgt tgccgacgcc ggcgatgaag
      541 ctctggtcga gcagcagcgt cttgagccgc gccgagcgac gggccagccg ttccgcgaag
      601 cgccgggccg tgaagccgtg gctcagcggc tccggtccat gcccgctgaa gacctcgccg
      661 atccggtagc gccggctccc ctccttcacc ttccgcgagc gcgctccgcg cccgccgcca
      721 acgctccgca ggcccccgcc gggccacagc ccgatccggc cgaacttccg gacgtcgcgg
      781 tagcgcagct cgcgcccgtc gctgagctcg aagacgactc tcgcgtacgg atccggcggc
      841 gccccgggtg gcgtgacgat cagcgccccc gtcatccgca gcgcgacggt catgacccga
      901 ccgtcggtga gatggatcag caccgtcttc gcccgccgcg tcacccgctg gatggtggcg
      961 ccggcgatct ccgccacgaa ccgctcgggg ggctgtgggt ggcggatggt gcggtcccag
     1021 gtgacggtcg cctcccggat ggtggcgccg gccacccact gctgcaggtc gcgggcgacc
     1081 gtctcgacct cgggaagctc cggcatgccg acgagtgtgc cgcatccgcc cgccacgcgc
     1141 gcagcgcctt acctgtcgcc tggcgaccac ggcagatgag acaggtacgt cctcgctgcg
     1201 gcggcggcaa ccgtccgcgc ctagacgagg tcggcggcga cctcggcgtc ggcctcctcg
     1261 cgggccacgt ccacggcggt cttcgggacg cgccgccact caccggcgtc ggagcggagg
     1321 tatcggtcca tctgctccca gttgcgtccg accttcaggt cgacctcgag cgggacgtcc
     1381 agcgggaggg cgctctccat gatctccttg acgagcccgg cgagctcggg aagctcgtcc
     1441 tcatcggtct cgaacacgag ctcgtcgtgc acctggagca gcatcttcga gcgcaggtcg
     1501 cgctcgcgca gcgcctggtc gacgcgaacc atcgcgatct tcatgccgtc ggccgcggtg
     1561 ccctggatcg gcatgttgat cgccatccgc tcgccggcgg agcgcagcgc gccgttgcgc
     1621 gcggtgagct ccggcaggta ccggcgccgt ccgaggagcg tggtgacgta cccctggtcg
     1681 cgggccagga tcttcatctc gacggtgtag cggcggatgc cctcgtaggc cgcgaagtag
     1741 tccgcgatga accgctgcgc ctcctcgcgc gggatcttga gccgctccgc caggccgaag
     1801 tcggacagcc cgtacgcgat cccgtagttg atcatcttcg ccacgctccg ctccttgggt
     1861 ccgacctggt cgggcgcaag cccgagcacg cgggcggcgg cggcgaggtg gatgtccgag
     1921 tgcgaggcga agtcggcgcg cagcccgggg tcggcggaga cgtgggccag gatccggagc
     1981 tcctgctgcg agtagtccgc gccgagcagc agcttgccct ccggcgcgac gaacgcgcgc
     2041 cggatgcgcc gccccagcgg cgtgcggatc gggatgttct gcaggttcgg atcggtgctc
     2101 gagaggcggc cggtggcggc caccgcctgc tggtaggtcg tgtgcagccg tcggttcgcg
     2161 tccaccaggc cgggcagcgc gtcgacgtac gtcgacttga gcttcgagac ctggcgatgc
     2221 tcgaggatca ggccgatgac ctcgtgtttg tcgcgaagct cctccaggac ggatgcatcg
     2281 gtcgagtagc ccgtgcgcgt ccgcttcgtg gccggcagcc ccagctcgtg gaagagaatc
     2341 tgctccagct gcttgggcga gccgatgttg aaggggcgcc ccacgaggtc gtagatgcgc
     2401 gcctccagac cggtcagctg cgcgccgaac tcctcctgca tggcggcgag cgcgtccagg
     2461 tcgatctcga tcccagccag ctccatgcgg gcgagcaccg ggaccagcgg catctcgacc
     2521 tcgtggaaca ggttcgtgag cgccgcgtcc gccagctccg actcgagcgc gggcttcgac
     2581 aggagcgcag tgagggcctc gccagcggcc cgacgcgcgc acccaacctc ctcggccccg
     2641 gtctcgggcg tgagcggctt cggcggcagc tggccgccga atcgggtcgc ggcaacgtca
     2701 tcgagggtct gcgcccgcag cgccgggttg accatgtagc ccgccacgag ggtgtcgaag
     2761 caggcgccgg ccagcgggac gtcgcgcgac ccgagcatca ccatcagctg tttcaggtcg
     2821 tgcccgacga ggtcgcggtc ctcgcgtccg aaccagccgg gcaacggccg gtcctcggtc
     2881 cacggcacgt accaggagct cccgtcgccg ccggccagcg cgaccccgaa cagcggccgc
     2941 tccagcggcc gccccgcccc ggccgaccac ccgatcccga tcccgccctc gtgtgcggcc
     3001 atccacgtct cgagctcggc gcggtcggcg tccgtccgca cgatcctcgg atcgacgtgc
     3061 tcgatcgcgc cggccgggtc cgcgaggggg tcgggtcgcg accgcggctc gtcgctcgga
     3121 gcggccgccg gcccgccgga cggcgcggca ccgcccagca ggtccaggct cagctggagc
     3181 ccgcccttcg tctcctccgg gccgtcgtag ccggtcggcg cgatggtcga tggcggcaga
     3241 cgatcgatca gcgatcggaa ctcgagctcc cgcatgcgct gggccaccgc gcggcggtcg
     3301 tatcggcccg tccgtgcctc ctcgaggtcc agctcgaccg gcaggtcgca cacgatccgg
     3361 ccgatctcgc gcgacatgaa ggcggacgcc cgatgctccg ccagccggtc gcggagcttg
     3421 cccttcacct cgtccaggcg ctcgtagatg ccgtcgaggg tgccgaactg ctggaggagg
     3481 ctgatcgccg tcttctcccc cacgcccggg acgccgggga tgttgtcgga cgtgtcgccc
     3541 ttgagggcct tgtagtcgag catctggtcc gggcgcaggc cgtagcgctc catgaccttc
     3601 gcctcgtcgt agatgaccgt ggcggcgacg cccatgcgtg tcgtcagcag ccgcacgtgg
     3661 tcgtcgacga gctgcagccc gtcgaggtcc ccggacacga tcatcgtgtc cacgccctgc
     3721 gctgtcgcct tcttgctcag ggtgccgatg acgtcgtcgg cctcgtagcc ctggagctcg
     3781 cggatcggca tgcccatcat ctgcgcgatc tcgcgcacga tcgggaactg cgcccgaagg
     3841 tcgtccggca tgctcggccg cccggccttg tactcggggt gcgcgtcgaa ccggaactgg
     3901 ggcttgccca cgtcgaagct gagcatcgcg tagtccggct tcacgtcctg catgcctcgc
     3961 agcacgatct gcaggaaccc gaagaccgcg ttcaccaggg tgccatccga cagcgtcaac
     4021 ggcggcaggg cgaagtaacc ccggtacacg aggctcggcc cgtcgatcag catcaggagg
     4081 ggtcggcgtt cgctcatcgg tcgcctgagt ctagaccgat acggccctcc gggccgccgt
     4141 agaatcggcc gatgcacagc cggccgagca tcgatccgta cgccgtcctg ggcgtcggtc
     4201 gcgaggcgac gccgctccag gtggcgcggg cgcaccgccg gctggccaag gcgcaccacc
     4261 ctgacctcaa cgagggtgcc agcgaggcct cggagcggat gcggcgcatc aacgaggcgt
     4321 ggtccatcct ctcgaatccc acccgtcgcg ccgagtacga ccgatcgcat cccaccgccg
     4381 gacggccgcc gggatcccac tgggccggca cccgctcccc catccagccc gcgtcaccga
     4441 cctcgacgcg gacctgggcc tcgtggcgcg cgaccgccga tcagacgcgg gcggccccgc
     4501 ggacggtgcg cgcaccgggg gagcggccgg tcccgcgcac gcggcgtcca ccgcgccccg
     4561 atccgatgcc cacgaccttc cgcgactcgc catgggcggc catcctcgcg gccgcactca
     4621 tcgtggg
//
LOCUS       DASTYP010000252         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1235374, whole genome shotgun sequence.
ACCESSION   DASTYP010000252 DASTYP010000000
VERSION     DASTYP010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235374"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFV59_10930"
     CDS             <1..1200
                     /locus_tag="VFV59_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326385.1"
                     /translation="TRLAQAEADEDAEPATSTAEQAVPWLIGVILLLAGMVIVLLALI
                     FAGDASLAGGGVEPSGSALAVVDPGGSGEAAATPSPTPVATRTPAPSVSTAPTPTPLA
                     MPDYGPLEMVYQGRSAALAAIYLLHRDFTTEDEPEVLAQDATLDVRRFAWSPDGRHGA
                     GLLADVLVSIEPGADKRRLGDGIETIVFGADPATLYAVRVTQDGANDVAAVLAIDFES
                     GDTTELASVTYARPSLEDEAAVNEAQFADDGGAVRLYWMHDGTLRLWALGAGSWAIDP
                     DAGEATELDTEDVPWLWDSDGRHRITYSASEGTTTISLVERNGETQATTTVEGLVSHL
                     RWSPDGKRVVFTVGRSTSGGGVLQDLFLWDLGDGEAPVQITNTGAAFGAQWLGTMDRF
                     QEASPDA"
     gene            1193..1537
                     /locus_tag="VFV59_10935"
     CDS             1193..1537
                     /locus_tag="VFV59_10935"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEU5326386.1"
                     /translation="MPDFVNPIGVELDETARRMRIRWDDGHLGEWSWLRLRRACPCAL
                     CAGEGNLPGVVTLETVFSEPQQTLTEVRWIGRYALAPIWADGHDTGLFTYGKLRELCE
                     CPEHAGAGAGGT"
     gene            1541..2404
                     /locus_tag="VFV59_10940"
     CDS             1541..2404
                     /locus_tag="VFV59_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5326387.1"
                     /translation="MHRSLHDGFTLALGGGGARGWAHIGVARALESLDLRPARIVGTS
                     MGAIIGAGLAAGRSADEMEADALRTPVLERMTRPARLAWYDARALIRGVVAELGNPRI
                     EELPTPLGITTFDLVTGRASLITTGPLDEALVRSIAVPFMLPPRCEGDAVWCDAGPWE
                     GVPVSHARAWDPEHPVVGVLADVTKPGVLSSRVGARLLRTAAHRLHREGSTTGRLTAR
                     RYLALVLERYADPVVDEAPDLLIRPRLGMMSALSFSHVQPAIDIGERDAREALRSYLA
                     IGAAEASVHAA"
     gene            2394..3377
                     /locus_tag="VFV59_10945"
     CDS             2394..3377
                     /locus_tag="VFV59_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU5326388.1"
                     /translation="MPPEAREHGGVVFVHAHPDDESMGSGGTIARLVAEGHRVDLVTC
                     TDGAEGEIHDPTLNHEEARPRLAEIRRDELACSIDALGDGKVKLHLLGYRDSGMMGTE
                     PNGHPEAFWNADLEAATRRLVEIVRRVRPAAIVSYDENGNYGHPDHINAARIAREAYA
                     ASGDSTRFPEAGTPCEVDRFYEIAFHRESWFGLMRAMQERGIALPWDLETAMADAPPA
                     DELNPTNAAALGQVAEAIEAGDEDRWAGFGRSDAEVTTRVDVSAYVEQKRRSMDCHRT
                     QRQDMGWLLDLPSDLAAAALSTEHFVLRWLGGLEVPSDYRETWLLPSEATA"
BASE COUNT          503 a         1241 c         1309 g          476 t
ORIGIN      
        1 acgcgcctgg cgcaggccga agcggacgag gatgcggagc cggcgacgtc gacggccgag
       61 caggcggtgc cgtggctgat cggcgtcatt ttgctgctgg ccggcatggt gatcgtgctg
      121 ctggcgctga tcttcgccgg cgatgcgtcg ctcgctgggg gtggcgtcga gccctcgggg
      181 agtgccctcg ccgtcgtgga ccccggtggc tccggggaag cggccgccac cccatcaccg
      241 acgccggtgg cgacacggac ccccgcgcca tcggtctcga ccgcgccgac accgaccccg
      301 ctggccatgc cggactacgg cccgctcgaa atggtctacc agggtcggag cgccgccctg
      361 gcggcgatct acctgctgca ccgcgacttc accaccgagg atgagccgga ggtgctggcg
      421 caggacgcga cgctcgacgt gcgccgcttc gcgtggtcgc ccgacggccg tcacggggcg
      481 ggcctgctgg ccgacgtgct cgtctcgatc gagcccgggg cggacaagcg gcggctgggc
      541 gacgggatcg agaccatcgt gttcggcgcc gatcctgcga cgctctacgc cgtgcgcgtg
      601 acccaggatg gcgccaacga cgtcgccgcg gtgctggcca tcgacttcga gagcggcgac
      661 accacggagc tcgcatcggt cacctatgcg cgaccgagcc tggaggatga ggcggcggtc
      721 aacgaggcgc agttcgcgga cgatgggggc gctgtacggc tgtactggat gcacgacggc
      781 acgctgcggc tgtgggcgct cggcgccgga tcctgggcga tcgatcccga cgcgggcgag
      841 gcgaccgagc tggacaccga ggacgtgcca tggctctggg actcggacgg tcgtcaccgc
      901 atcacgtact cggccagcga gggcaccacg acgatctcgc tggtcgaacg caacggcgag
      961 acgcaggcga cgacgaccgt cgagggcctg gtcagccacc ttcgctggtc gccggacggc
     1021 aagcgcgtgg tcttcaccgt gggtcgctcg acgtccggcg gcggcgtgct ccaggacctg
     1081 ttcctgtggg atctcgggga cggcgaggca ccggtccaga tcacgaacac cggcgccgcg
     1141 ttcggcgcgc aatggctcgg caccatggac cgattccagg aggcctcgcc cgatgcctga
     1201 cttcgtcaac ccgatcggcg tggagctcga cgagaccgcg cggcggatgc gcatccgctg
     1261 ggacgacggg cacctgggtg agtggtcgtg gctccggctc cgtcgggcct gcccgtgcgc
     1321 gctgtgcgcc ggcgagggca acctccccgg cgtcgtcacc ctggagacgg tcttcagcga
     1381 accccagcag acgctgaccg aggttcgctg gatcggccga tacgccctgg ccccgatctg
     1441 ggccgatggg cacgacaccg gcctcttcac ctacggcaag ctgcgcgagc tgtgcgagtg
     1501 ccccgagcac gccggagccg gcgcgggagg cacctgaccg atgcatcgct cgctgcacga
     1561 cggcttcacg cttgccctcg ggggcggagg ggcgcgaggc tgggcgcaca tcggcgtcgc
     1621 ccgagcgctc gagtcgctcg accttcgccc cgcccggatc gtcgggacga gcatgggcgc
     1681 catcatcggc gcgggcctgg ccgcaggtcg ctcggccgac gagatggagg cggacgcgct
     1741 gcgcacgccg gtgctggagc gcatgacgcg tcccgcgcgg ctggcctggt acgacgcgcg
     1801 agccctgatc cgcggcgtgg tggccgagct cggcaacccg cggatcgagg agctgccgac
     1861 gccgctcggc atcaccacct tcgacctggt gaccggccgc gcgagcctga tcaccacggg
     1921 gccgctggac gaagcgctgg tccggagcat cgccgtgccg ttcatgctgc cgccgcggtg
     1981 cgagggcgac gcggtatggt gcgacgccgg cccgtgggag ggggtgcccg tctcgcatgc
     2041 gcgcgcctgg gatcccgagc atccggtggt cggggtgctc gccgacgtca ccaagcccgg
     2101 cgtgctgagc agccgcgtcg gcgcgcgcct cctccgcacc gcggcgcatc ggctgcaccg
     2161 ggagggttcg acgaccggac ggctcacggc gcgccgctac ctcgcgctgg tcctcgagcg
     2221 ctatgcggat ccggtggtcg acgaagcacc ggacctgctg atccggccac ggctcggcat
     2281 gatgagcgcc ctctcgttca gccacgtcca gccggccatc gacatcgggg agcgcgacgc
     2341 gcgcgaggcg ttgaggtcgt acctggcgat cggcgcagcg gaggcatcgg tccatgccgc
     2401 ctgaggcccg cgagcacggc ggcgtcgtgt tcgtgcacgc ccaccccgac gacgagtcga
     2461 tgggcagcgg cgggacgatc gcgcggctcg tggcggaggg ccatcgcgtc gacctggtca
     2521 cctgcaccga cggcgcggag ggtgagattc acgatccgac cctgaatcac gaggaggcgc
     2581 gcccgcgcct ggcggagatc cgccgggacg agctggcgtg ctcgatcgac gcgctcggcg
     2641 acggcaaggt caagctgcac ctgctcgggt accgtgactc cgggatgatg ggcaccgagc
     2701 ccaacggcca ccccgaagcg ttctggaacg cggacctcga ggcggcgacg cgacgcctgg
     2761 tcgagatcgt gcgccgggtt cggccggcgg ccatcgtgag ctacgacgag aacggcaact
     2821 acgggcaccc tgaccacatc aacgcggcgc ggatcgcgcg cgaggcgtac gcggcatccg
     2881 gcgactcgac ccgcttcccg gaggccggga cgccgtgcga ggtggaccgc ttctacgaga
     2941 tcgccttcca tcgcgagtcg tggttcggac tgatgcgcgc catgcaggag cgcggcatcg
     3001 ccctgccgtg ggacctggag acggccatgg ccgatgcgcc acccgccgac gagctcaacc
     3061 ccaccaacgc ggccgcgctg gggcaggtcg ccgaggcgat cgaggcgggg gacgaggacc
     3121 gctgggcagg cttcggccga tccgacgccg aggtgaccac gcgggtcgac gtgtccgcct
     3181 acgtcgagca gaagcgccgc agcatggact gccaccgcac gcagcgccag gacatgggat
     3241 ggctcctcga cctgccgtcg gaccttgcgg ctgccgcgct gtcgacggag cacttcgtcc
     3301 tgcgctggct gggcgggctg gaggtcccct ccgactaccg cgagacctgg ctgctcccgt
     3361 cggaggcgac ggcgtgagca gcgaccccct tcccatgacc gatgcgctgc tggactacgt
     3421 ccgcagcgtg ggcgtccgcg aacacccggt cctggccgag ctccgcgagg agacgcagcg
     3481 cctgccgcag gccagcatgc agatcgcgcc cgagcagggc gagttcatg
//
LOCUS       DASTYP010000253         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_615906, whole genome shotgun sequence.
ACCESSION   DASTYP010000253 DASTYP010000000
VERSION     DASTYP010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615906"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /gene="rpsO"
                     /locus_tag="VFV59_10950"
     CDS             <1..223
                     /gene="rpsO"
                     /locus_tag="VFV59_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU5326389.1"
                     /translation="THEGDTGSPEVQIALLTERIKSLTEHLRSFPKDNHSRRGLLKLV
                     GQRRRLLAYLVRNDVDRYRAVISSLGLRR"
     gene            427..2991
                     /gene="pnp"
                     /locus_tag="VFV59_10955"
     CDS             427..2991
                     /gene="pnp"
                     /locus_tag="VFV59_10955"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU5326390.1"
                     /translation="MEAGKLAEQADGAVIVRYGDTVVLATAVASKEPREGADFFPLTV
                     DYEERMYAAGKIPGGFIKRESRPSEAAILAMRLTDRPIRPLFPKGYFNDVQIVLTVLS
                     TDMENDPDILALNGASAALSISHIPFMGPVGAVRVGRLNGEFVTNPTNTQLDESDLDL
                     VVAGTREAVMMVEAGAKILPESVMADAIAYGHAELQKSIDLQEKLVASAGTPKKMPFV
                     GPKAASIVKLGKRLADGQTEFVVVDLETTAMKPEHGYIVDIAALRVRDGKVVDRFESL
                     VNPGRPIVGHQVHGITTDDVANAPIASEVLGRLVEWIGDAPVVAHNIAFDLPYILRHL
                     PPEVKWEPKELYDTLELGYQLYPDAGVWKLGDLVRFVFGRDHAAAHRAMPDAEATAEL
                     FIHFTQGLAERLDSIREDIAGEIRRGRENYNRSEQGERLEDIRRRHGIGSALMDVMTK
                     STVRELVLSEDIRIDGRDTKTIRPISVEVGVLPRTHGSAIFTRGQTQALSIATLGPSS
                     AVQRMDTISPETEKRYLHHYNMPPYSVGEARFMRGPGRREIGHGALAERALLPVLPTQ
                     DEFPYVIRVVSEVVSSNGSTSMASTCGSTLALMDAGVPIKAPVAGAAMGLITDPETGR
                     YAVLTDINGKEDAYGDMDFKVTGTAEGVTALQMDIKVAGITTEIMRDALEQARVARLF
                     ILEKMTNVISASREELSPFAPRITTIKIPVDKIRDVIGSGGKVIRQITAETGTEINIE
                     DDGTIQIAATSGEAAQKAIKWIEGLTKDVEVGKEYLGKVTRIMNFGAFVEILPGKEGL
                     VHISQLADYRVPRVEDVVSIGDELMVVVTEIDRMGRVNLSRRAAMERHMAKAGTSS"
     gene            3011..3712
                     /locus_tag="VFV59_10960"
     CDS             3011..3712
                     /locus_tag="VFV59_10960"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5326391.1"
                     /translation="MGTASEPAGSAAALAQVADEVRGCTRCPLAARRTHTVPGEGNVL
                     SDVLLVGEGPGAREDATGRPFVGPAGQLLDELLGSIGWRRDDVFITNVVKCRPPGNRD
                     PEPGEIAACGGYLARQEAALEPAVVVTLGRHSLQRYLPGSRIGAVHGGLRRSYSGLHV
                     FPMYHPAAALHQASLRETLFHDMRGLPAALLAARQALDEERAAAVQAEAAVTPGEPRP
                     VSVDDAQQMTLFQEH"
     gene            3713..>4373
                     /locus_tag="VFV59_10965"
     CDS             3713..>4373
                     /locus_tag="VFV59_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5326392.1"
                     /translation="MSGSLRIIPLGGLGEVGKNITAIEVDGEILVVDCGLAFPEPEML
                     GIDLVIPDATYLAEHRDQVRGIVLTHGHEDHTGSLPYVLPKIPGTPIYATRLTQGLVQ
                     GKLREHKLLDSTPLHVVEPGVEFTVGSFRITPFRVNHSIPDGVGYAIETAYGTIVHTG
                     DFKFDHTPPDGRRADLGLIAAIGNRGVDFLLSDSTRAEKEGYTLSESTVGESLHRLVG
                     EA"
BASE COUNT          736 a         1562 c         1460 g          615 t
ORIGIN      
        1 cacgcacgag ggcgacactg gatcacccga ggtgcagatt gcgcttctca ccgagcggat
       61 caagtcgctg acggagcacc tgcgcagctt ccccaaggac aaccactcgc gccgcgggct
      121 gctcaagctc gtcgggcagc gtcgacggct gctggcctac ctggtccgca acgacgtgga
      181 tcggtaccgc gccgtcatca gcagcctggg gctgcgccgc taacaccgct cgcgatcgac
      241 ccaggccccc gcacgggacc tgggtctcgc acatctgctc acacacaccc tgagcggcat
      301 tcgaggaacc acctgacgcg gcgctccgag atggagcgcg ccagctggac tcgcgtgccg
      361 cgacacacac tggaggattc agtggccatc aagcacgagg ccgaaatcgg cggccaggtc
      421 ttcagcatgg aggccggcaa gctcgccgag caggccgacg gcgcggtcat cgtccgctac
      481 ggcgataccg tcgtcctggc gaccgccgtc gccagcaagg agccccgcga gggcgccgac
      541 ttcttcccgc tcaccgtcga ctacgaggag cggatgtacg ccgcgggcaa gattcccggc
      601 ggattcatca agcgcgagtc gcgcccgtcg gaggcggcca tcctggccat gcgcctcacc
      661 gaccgcccga ttcgcccact cttcccgaag ggctacttca acgacgtcca gatcgtcctg
      721 accgtcctct ccaccgacat ggagaacgat ccggacatcc tggcgctcaa cggcgcctcc
      781 gcggcgctgt ccatcagcca catcccgttc atggggccgg tcggcgcggt gcgcgtcggc
      841 cgcctgaacg gcgagttcgt gacgaacccg accaacaccc agctggacga gagcgatctc
      901 gacctggtcg tcgccgggac ccgcgaggcg gtgatgatgg tcgaggcggg tgccaagatc
      961 ctgccggagt cggtcatggc cgatgcgatc gcctacggcc acgccgaact ccagaagtcc
     1021 atcgacctgc aggagaagct ggtcgccagc gccggtacgc ccaagaagat gccgttcgtc
     1081 ggcccgaagg ccgcgtccat cgtcaagctg ggcaagcgcc tcgccgacgg ccagaccgag
     1141 ttcgtggtgg tcgacctcga gaccaccgcg atgaagccgg agcacggcta catcgtcgac
     1201 atcgccgcgc tgcgggtgcg cgacggcaag gtggtggatc ggttcgagtc gctcgtgaac
     1261 cccggccgcc cgatcgtcgg gcaccaggtc cacggcatca cgaccgatga cgtcgccaac
     1321 gcgcccatcg cctcggaggt cctcggccgc ctggtcgagt ggatcggcga tgcgccggtg
     1381 gtcgcccaca acatcgcctt cgacctgccc tacatccttc gccacctgcc gcccgaggtg
     1441 aagtgggagc cgaaggagct gtacgacacc ctcgagctcg gctaccagct ctaccccgac
     1501 gcgggcgtct ggaagctggg tgacctggtg cggttcgtct tcgggcgcga ccacgccgcc
     1561 gcgcaccgcg cgatgcccga cgcggaggcc accgccgagc tgttcatcca cttcacgcag
     1621 ggcctcgccg agcggctcga ctcgatccgc gaggacatcg ccggcgagat tcggcgcggc
     1681 cgggagaact acaaccggtc ggagcagggt gagcgcctgg aggacatccg gcggcgccac
     1741 ggcatcgggt cggccctcat ggacgtgatg accaagtcga ccgtgcgcga gctcgtgctg
     1801 agcgaggaca tccggatcga cggccgcgat accaagacca ttcggccgat cagcgtggag
     1861 gtcggggtcc tcccgcgcac ccacggctcg gccatcttca cccgcggcca gacgcaggcg
     1921 ctctccatcg cgaccctggg gccgtccagc gcggtccagc gcatggacac gatcagtccc
     1981 gagaccgaga agcggtacct gcaccactac aacatgccgc cgtactccgt cggcgaggcg
     2041 cgcttcatgc gtggccccgg ccgtcgcgag atcggccatg gcgccctcgc ggagcgggcc
     2101 ctgctgccgg tgctgccgac gcaggacgag ttcccgtacg tcatccgcgt cgtgagcgag
     2161 gtggtgagct ccaacggctc gacctccatg gcctcgacct gtggctcgac gctggccctg
     2221 atggacgccg gtgtgccgat caaggcaccg gtcgccggcg cggcgatggg cctgatcacc
     2281 gatcccgaga ccggccgcta cgccgtcctg accgacatca acggcaagga ggacgcgtac
     2341 ggcgacatgg acttcaaggt gaccggcacg gccgagggcg tgacggcatt gcagatggac
     2401 atcaaggtcg ccggcatcac gaccgagatc atgcgtgacg cgctcgagca ggcgcgcgtg
     2461 gcccggctct tcatcctgga gaagatgacc aacgtcatca gcgccagccg cgaggagctc
     2521 agcccgttcg ccccgcgcat cacgaccatc aagatcccgg tcgacaagat ccgcgatgtc
     2581 atcggctccg gcggcaaggt catccgccag atcacggccg agacgggcac cgagatcaac
     2641 atcgaggacg acggcacgat ccagattgcc gcgacgagcg gcgaggccgc ccagaaggcg
     2701 atcaagtgga tcgagggcct gaccaaggac gtcgaggtcg gcaaggagta cctcggcaag
     2761 gtgacccgga tcatgaactt cggtgccttc gtcgagatcc tgcccggcaa ggaggggctg
     2821 gtccacatca gccagctcgc cgactaccgg gtgccgcgcg tcgaggacgt ggtgagcatc
     2881 ggcgacgagc tgatggtcgt cgtgaccgag atcgatcgca tggggcgcgt gaacctgagc
     2941 cgccgcgcgg cgatggagcg tcacatggcc aaggccggca cgagcagctg aaccgcatcg
     3001 gccgcgcgcg atgggcaccg cgagcgagcc ggcaggaagc gcggcggcgc tggcgcaggt
     3061 cgcggacgag gtccgtggct gcacgcgctg tccgctcgcc gcgcgccgca cccatacggt
     3121 cccgggcgag gggaacgtcc tgagtgacgt cctcctcgtc ggggagggcc cgggcgctcg
     3181 agaggacgcg accggccggc cgttcgtcgg accggccggg cagctgctcg acgagctgct
     3241 ggggtccatc ggctggcggc gggacgacgt gttcatcacg aacgtcgtga agtgccgtcc
     3301 accgggcaac cgtgacccgg agcccggcga gatcgcggcg tgtggaggct acctcgcgcg
     3361 ccaggaggcg gcgctcgagc ccgcggtggt cgtcacgctc ggtcgccact cgctccagcg
     3421 ctaccttccc ggctcgcgga tcggcgccgt ccatggtggg ctgcgtcgct cctactcggg
     3481 cctccacgtc ttcccgatgt accatcctgc tgcggcactg caccaggcct cgctgcgtga
     3541 aacgctgttc cacgacatgc gagggctgcc cgcggcgctg ctggcggccc gccaggcgct
     3601 cgacgaggag cgcgcggcgg ctgtccaggc agaagcggcg gtgaccccgg gcgaaccccg
     3661 tccggtctcc gtcgacgacg cccaacagat gaccctcttc caggagcact gaatgtccgg
     3721 atctctgcgc atcatcccgc tcggcgggct cggcgaggtc ggcaagaaca tcaccgccat
     3781 cgaggtcgac ggcgagatcc tcgtcgtcga ctgcggcctc gcgttccccg agccggagat
     3841 gctcgggatc gacctcgtca tccccgacgc gacgtacctc gcggagcatc gcgaccaggt
     3901 gcgcggcatc gtgctgaccc acggacacga ggaccacacc ggctcgctgc cgtacgtgct
     3961 gcccaagatc cccggcacgc cgatctacgc cacccgcctc acccagggac tcgtccaggg
     4021 caagctccgc gagcacaagc tcctcgactc cacgccactc cacgtggtcg agcccggcgt
     4081 cgagttcacg gtcggctcgt tccgcatcac gccgttccgc gtgaaccact cgatcccgga
     4141 cggggtcggg tatgccatcg agacggcgta cggcacgatc gtgcacaccg gcgacttcaa
     4201 gttcgaccac accccgcccg atggacggcg cgccgacctc ggcctcatcg ccgccatcgg
     4261 caaccggggc gtcgacttcc tgctctccga ttcgacccgc gcggagaagg agggctacac
     4321 gctctccgag tcgacggtgg gggagagcct gcatcgcctg gtgggcgaag cgc
//
LOCUS       DASTYP010000254        13120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1271909, whole genome shotgun sequence.
ACCESSION   DASTYP010000254 DASTYP010000000
VERSION     DASTYP010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13120
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271909"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..2745
                     /gene="ligD"
                     /locus_tag="VFV59_10970"
     CDS             133..2745
                     /gene="ligD"
                     /locus_tag="VFV59_10970"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEU5326393.1"
                     /translation="MPLEAYRSKRDFGRTAEPAGGEPGGDGRRFTVQRHRATALHYDF
                     RLEIDGVLVSWAVPKGPTLDPGERRLAMRTEDHPIEYLDFEGVIPGGQYGAGDVIVWD
                     WGHFEPDPETPDPAAAIRAGELKFSLHGEKMRGRFTIVRTDGRNRDPDREQWLLIHKR
                     DAHAVDGWTAEDHPASVKTGRTNDEVRDGIPPRFEAAPPAPVGEIDLSRADEEPMPDF
                     LPPMRATLASEPFDDPDWLFEVKWDGYRVEAVIRDGHARLWTRNRQDAAAYFPDLAGP
                     AAWIDAREAIVDGEVVALDEEGRPRFSLLQDRTGVRTGRAPGTRRKGTPAPIVFEAFD
                     LLHLDGRSLLHLPLEDRKRLLRSRLREHPLVRYAGHVEADGRAFFAAARQQELEGIVA
                     KLRRSPYEPGARSRSWLKLKARREQEAVVVGWLQGQGSHADLGSLILAVRSGDRWVHA
                     GQVGSGLDARTRRELRTQLDELARDDSPLDPAPRIRGAHWVDPRLVVRVEFAEWTADD
                     LLRQAAFKGVEVGRDPRDVRREREVSTASATAAAEAAIPQPPRSDTAPSATADELAAL
                     DALGSEGTWTVGGREVRVTNLDKVLFPPRPGDEQITKRDLLRYQLLIAPTILPYLAGR
                     GVTVHRYPNGVEHKGFWQKDLPSHTPAWVTRWTYDHGREGRKDYPVVDHPATLAWLAQ
                     EAAIELHPWTSPTDAPDRPSYALIDIDPGDDTSWEEVLVLARLYRTALGHIGVRGHPK
                     VTGQRGIQVWIGIRRGPTFDDTRDWVERLSRAVGSTVPDLVSWEWVKRARQGRARLDY
                     TQNAVNKTLVAPYSPRPAAGAPVSVPITWDELDDPALRPDRWTIRDVLHRIGDAGDPF
                     RPLLDDEQELPPLG"
     gene            complement(2805..3323)
                     /locus_tag="VFV59_10975"
     CDS             complement(2805..3323)
                     /locus_tag="VFV59_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326394.1"
                     /translation="MRRALGILPITALLLAACARGTGAGGSDASMAPPMSSSGASSAS
                     ASAAASVGESPAAGRIDIGALVGDAASYEGQDVTILGRVDSVLADGQAFTTSANGDDD
                     NILVIVGDNATIEKDITEKAVVFITGTVVPFTGDDLAAAGAAIGADQLSDYQGDYAIV
                     ATDIADPLNSGS"
     gene            3524..4108
                     /locus_tag="VFV59_10980"
     CDS             3524..4108
                     /locus_tag="VFV59_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU5326395.1"
                     /translation="MIETLLTVTPDARQKIDGVRTFNNFPEAVLRVRILGKDGPRFRY
                     EIALEDPRDRTAEDLSLDVDGLQVVVDATSAADLAGASITLDDAITGGALRVENPNEG
                     WGDPVAQAVQDVIDRQINPGVGSHGGMVSLVEVREGTAYLRFGGGCQGCAAVNVTLRQ
                     GVEQAILGSVPQVTAIADVTDHGAGENPYYRHGH"
     gene            complement(4152..4277)
                     /locus_tag="VFV59_10985"
     CDS             complement(4152..4277)
                     /locus_tag="VFV59_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326396.1"
                     /translation="MRTKRIIRSAMIAGTAAALSILSLVSTVMAVTGGGDFPLRR"
     gene            4444..5742
                     /locus_tag="VFV59_10990"
     CDS             4444..5742
                     /locus_tag="VFV59_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5326397.1"
                     /translation="MPRTRTAARATDKDRELAQRIGVRIREARHRAGLTQQQLAGERY
                     TKAYVSALETGIARPSMVALSYLSRRLGLPASHFIDEAHPAWTRLEVDMRLAAGEWTQ
                     AADGYRALLDDATDDMVRADLLRGLAEALARLDRGRETITAASESARLYAEAGRESDA
                     ALARYWLAYGLYQSDNEPDARSLLLSLLEQVRGGLAVEPDFEMRLLTALAAVESRSGQ
                     PARSLTYLEEARGLASDLDDRRRAAFLFNLAISYREVGDFEAAIRAGTQGMALYRAAG
                     AEFESARIENDLALAYLATGQVSRARDLAADARRQIEESGDERWLAAVIDTEAQVALA
                     DGDAEGALRLSALAREHATRSGNELARLAAVITEARALRRSGRDPEAEDRFRTAAEIA
                     RTSRVPSRLKDVLREWADLRAEAGDHRGAYELTSEALAVN"
     gene            complement(5875..6465)
                     /locus_tag="VFV59_10995"
     CDS             complement(5875..6465)
                     /locus_tag="VFV59_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326398.1"
                     /translation="MRQRLTGRPREQPMARRPEDARRLSIPPAARGLVGWAAVALLLV
                     AIAFGARLIGTAGDEAVALQSASPSATAGPSAPAIAFGTAIDAVTGAVAADSATTTFG
                     AGDDFAYSVRPALPPPPTIYVEVLRTGSDPATVQPASPQTLPDGAALIAFVVPADALI
                     ADFGPGDYVMRIFAQPTEAPIAIGTFTLAGAAQPSS"
     gene            6552..7079
                     /locus_tag="VFV59_11000"
     CDS             6552..7079
                     /locus_tag="VFV59_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEU5326399.1"
                     /translation="MARRLFILDDPDRFVAGTVGEPGDRAFYLQARKGSTVVSVGLEK
                     AQVAALADRLDDLLDAVEAPPRPTATDEGDLDRPIVEMFRVGAMALAWDAASESVVIE
                     AQTPTEDGEYAELPDDAEDGPDLLRVRIDPGTARAFVRRAEALVSAGRPLCPFCGQPL
                     DPQGHFCARSNGHLN"
     gene            7085..7648
                     /locus_tag="VFV59_11005"
     CDS             7085..7648
                     /locus_tag="VFV59_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HEU5326400.1"
                     /translation="MAEPLDPPLRVDWIEAAELGDGRPGRLGLTFLPGKRGASLRYPG
                     LVYRRELASDVRTLLEDGVTRLVLLVEDTELERWGDPDIVQAAAAAGLDVRRFPMPDG
                     EPPTSIEEMDAILAALDEARVDGDAAVACMGGVGRTGTVAACALVRAGVPPRDAIERV
                     RDVRHPEAVETRSQERFVAAYAARRHA"
     gene            7668..8231
                     /locus_tag="VFV59_11010"
     CDS             7668..8231
                     /locus_tag="VFV59_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU5326401.1"
                     /translation="MRPERVLGIALVLIVVAAAAVGVWIALGSGTNAVPADGGTGPML
                     ASPPAGEPARIERVIDGDTVVVDLDGRRERVRYIGVDAPELANAEAGIAAECWGQEAR
                     QANERLVDDAEVLLERDVSDRDRFGRLLRHVWVSEDGGWRLAAEELVAIGAVEARAYP
                     PDTARDGELDRAERAARRDGRGLWGGC"
     gene            complement(8228..11746)
                     /locus_tag="VFV59_11015"
     CDS             complement(8228..11746)
                     /locus_tag="VFV59_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEU5326402.1"
                     /translation="MQLIDGTPVFSATDLVGYLACEHLTALERSVLGHPELKPHRVDR
                     ELDLIRRHGELHEARYLEQLEADGRRVVRIEKDAGEGRDRLERQARATVDAMASGADV
                     IYQATFFDGRWLGYADFLLRVEADDRDRASVWGPYHYEVADTKLARHVKASAVLQICS
                     YVEQLSAIQGVQPERLRVVLGGGRDPVELRVDDYMAYYRAARRRFEAAFELPPGTTYP
                     EPVDHCDVCRWWQVCADRRREDDHLSLVAGISAGQRRALTAGGIDTVVKLANAPIPLP
                     ELDGASAAALDRIQRQAAIQVRGRAVAPQLLYELITPVEAERGLAVLPEPDPGDLFLD
                     LEGDPYAFGDSFDYLFGLLDVDGAFTAIWSRDPDRPEEFSLAGEKAAFERVMDLITER
                     LERYPNMHVYHYAAYEPTALKRLMGRHATREEAVDRLLRGGVLVDLFRAVRQGVRASV
                     ESYSIKRLEPLYGFERAIPLKDATSSIVAFEEWLQLADTERPSSAILDEIAAYNRDDV
                     VSTLRLRDWLEARRRELETMTGAPVPRPVPQSPEPAEKLSEVAARVEEVGARLTDGVP
                     ADEAERSAEQQARWLLAQLLSWHRREMKATYWEFFHRIGMTSDELVTDDVALGRLRLI
                     GPDGEPYRPTPKSKWRQDWRYAMPPQEHDIGDRSELYDPALQQVFPDEMKRWRIKGKL
                     KEIDDRRESIVLTWNVDVEPQHPTAIVPLNVIGDNDHRAALLRLGEWVADHGVDADGP
                     ARAARDLLLRRPPRCGQAAGAGLKLPGESDLEAACRLALALDHSTLAIQGPPGSGKTF
                     TGARMIVALLRAGKRVGISATSHKVITHFLEEVLCAADAVGGVDVRAAQKVSDGVTGL
                     DDPRVRVEGEAGPIRQGLADGELNLVAGTSWLWVPETSDGLLDVLFVDEAGQISLANV
                     VAMGGAADSLVLLGDPQQLDQPLQGSHPPGADRSALAHLLGEQQTMPPHLGLFLEDTW
                     RMHPDITAFTSSAFYERRLESRPQLATQWLRAPAPVGGTGVRLITAEHAGADNASPEE
                     AQQVADLVRALVQSGSAWTDADGEQHPLRWNDVLIVAPYNAQVGEIQRLLPDARVGTV
                     DKFQGQQAPISIYSMTTSSPELAPRGMDFLYSRNRLNVATSRARCVAVVVASPELLRV
                     RARTPEQMRLANALCQFVELAEGLVD"
     gene            complement(11807..12604)
                     /locus_tag="VFV59_11020"
     CDS             complement(11807..12604)
                     /locus_tag="VFV59_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5326403.1"
                     /translation="MGTRARAASLCPLVLLTACTFAPLPTPTPTPSPTPTPTPSPSPT
                     PTPVPTPTPEPTPDVGAMPRIPGGEVATTTIDGLRVRQRPGTSTVVLTGLMPLGSEVG
                     VVMGPVLVDGQGWFLVTDVDPAEPAFSEGWIASGLGPEPYLAATGRAVEGSPVLASYA
                     ELGDAEYGPIDLGDGAHAVRWIAVDPEGRRCQFSVAMTDTDSTDVPAIRATVGNDVVP
                     GTLQPQSFAALGITGLAFVTITSDCDWALAVVRVPDETPQPSASAAP"
     gene            complement(12622..>13120)
                     /locus_tag="VFV59_11025"
     CDS             complement(12622..>13120)
                     /locus_tag="VFV59_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU5326404.1"
                     /translation="FLQYPRWFFPTSESREEIRRAKVRLGDTPFAVELRHASWFNEKN
                     LERTIRFFEDERIPFVMVDAPPGTKSSLPPMTVVTSPKLAVVRFHGRRTETWEKSGIP
                     VVERFRYLYGPEELGEWVPRIRETAKQVPEVHVLMNNCYANYGTTNAREIADLLASEV
                     PRLDR"
BASE COUNT         1839 a         4583 c         4841 g         1857 t
ORIGIN      
        1 accgcggcgg gcatgctgcc gatcgccgtc atggcgggcg ctgcggtcga gcgcgaggtc
       61 ctcgtggcgg ctggcgcgcg cgctgtgctc gagacgctcg acgagctgac cgatcgcctg
      121 cccgcctgac cgatgccgct cgaggcctat cggtccaagc gcgacttcgg caggaccgcc
      181 gaaccggccg gtggcgagcc tggaggagac ggccgccgct tcaccgtgca gcgtcaccgt
      241 gcgacggcgc tccactatga cttccggctc gagatcgatg gcgtgctggt cagctgggcg
      301 gtgccgaagg gtccgaccct cgaccccggc gagcgccggc tggccatgcg gaccgaggat
      361 cacccgatcg agtacctgga cttcgagggc gtcatcccgg gcggacagta cggggcgggc
      421 gacgtcatcg tgtgggattg gggtcacttc gagcccgatc ccgaaacgcc cgatcccgca
      481 gcggcgatcc gggccggcga gctgaagttc agcctgcacg gcgagaagat gcgcgggcgc
      541 ttcaccatcg ttcgcaccga tggacgcaac cgcgaccccg accgtgagca gtggctgctg
      601 atccacaagc gtgacgcgca cgcggtcgac ggctggacgg ccgaggacca cccggccagc
      661 gtcaagaccg gtcgcaccaa cgacgaggtg cgcgacggca tcccgcctcg cttcgaggcc
      721 gcacctcccg caccggtggg cgagatcgac ctgtcgcgcg cggatgagga gccgatgccc
      781 gacttcctcc cacccatgcg cgccaccctc gccagcgagc cgttcgacga tccggactgg
      841 ctgttcgagg tcaagtggga cggctaccgc gtggaggccg tcatccggga cggtcatgcc
      901 cggctctgga cgcgcaaccg gcaggacgcc gcggcctact tcccggacct ggccggtccc
      961 gcagcctgga tcgacgcccg cgaggcgatc gtggatggcg aggtggtggc gctggacgag
     1021 gagggacggc cacgcttcag cctgctccag gaccgaacgg gcgtgcggac cggtcgcgcc
     1081 ccgggcacac ggcgcaaggg aaccccggcg ccgatcgtgt tcgaggcgtt cgacctgctg
     1141 cacctcgacg gccgcagcct gctccacctg ccgctggagg atcgaaagcg gctgctccga
     1201 tcacggttgc gcgagcatcc gctggtccgc tacgccggcc acgtcgaggc cgatgggcgc
     1261 gccttcttcg ctgccgcgcg gcagcaggag ctggagggca tcgtggccaa gctgcgccgg
     1321 tcgccgtacg agcccggcgc gcgcagccga tcgtggctga agctcaaggc ccgccgcgag
     1381 caggaggccg tcgtcgtcgg ctggctgcag ggacagggat cccacgccga cctgggttcg
     1441 ctcatcctcg ccgtgcgctc cggcgatcgc tgggttcatg ccgggcaggt cggcagcggg
     1501 ctggacgccc gcacgcgccg cgagctgcgc acccagctcg acgagctggc tcgtgacgac
     1561 tcgccgctcg atccggcgcc gcgcatccgc ggtgcgcact gggtcgatcc tcggctcgtc
     1621 gtgcgcgtcg agttcgcgga gtggacggcg gacgacctgc tgcgacaggc ggcgttcaag
     1681 ggcgtcgagg tcggacggga tccgcgcgac gtgcggcggg agcgggaagt gtccacggcg
     1741 agcgcgaccg cggcggccga ggcggcgatc ccgcagccgc cccgctcgga cacggcgccg
     1801 tccgcgaccg cggacgagct ggccgccctc gacgcgctcg gctcggaggg gacctggacg
     1861 gtgggcgggc gcgaggtgcg cgtcacgaac ctcgacaagg tgctcttccc gccgcgcccc
     1921 ggtgacgagc agatcaccaa gcgcgacctc ctgcgctacc agctcctgat cgcgccgacc
     1981 atcctgccgt acctcgcggg ccggggtgtg accgtgcacc gctatccgaa cggcgtcgag
     2041 cacaagggct tctggcagaa ggacctgccg tcgcacacgc cggcgtgggt gacccgctgg
     2101 acctacgacc acggtcgcga gggccgcaag gactacccgg tggtggacca cccagccacc
     2161 ctcgcgtggc tggcccagga ggccgcgatc gagcttcacc cctggaccag cccgaccgat
     2221 gcgcccgacc ggccgtccta cgccctgatc gacatcgacc cgggcgacga cacctcgtgg
     2281 gaggaggtcc tggtgctcgc ccgcctgtat cggaccgcgc tgggccacat cggcgtccgc
     2341 ggccacccca aggtcaccgg acagcggggg atccaggtct ggatcgggat ccggcgcgga
     2401 ccgacgttcg acgacacccg cgactgggtc gagcggctgt cgcgggccgt cgggtcgacg
     2461 gtgccggacc tggtcagctg ggagtgggtc aagcgtgcgc gacagggacg ggcacggctc
     2521 gactacaccc agaacgcggt caacaagacg ctcgtggcgc cgtacagccc acgtccggca
     2581 gccggagccc cggtcagcgt cccgatcacg tgggacgagc tggacgaccc cgcgctgcgt
     2641 cccgaccggt ggaccattcg cgacgtgctg caccgcatcg gcgacgccgg cgatccgttc
     2701 cgcccactgc tcgacgacga gcaggagctg ccgccactgg gatgacacga cgacgccgcc
     2761 cttcgcgtcg gcgagaggcg gcgtcgcatg ggaaggctgg gcgcttagct gcccgagttc
     2821 agcgggtcgg cgatgtcggt cgccacgatg gcgtagtcgc cctggtagtc gctcagctgg
     2881 tctgcgccga tggcggcgcc agccgctgcg agatcgtctc cggtgaaggg cacgaccgtg
     2941 cccgtgatga agacgacggc cttctcggtg atgtctttct cgatcgtcgc gttgtcccca
     3001 acgatcacga ggatgttgtc gtcgtcgccg ttcgcggacg tcgtgaacgc ctggccatcg
     3061 gccagaacgg agtccacgcg gccgaggatc gtgacgtcct ggccctcgta ggaggcggca
     3121 tcgccgacga gagcgccgat gtcgatgcga ccggcagcgg gtgactcacc cacgctcgcc
     3181 gcggcgctgg cggaggcgct ggacgcgccg ctggatgaca tcggcggcgc catcgaggcg
     3241 tcgcttccgc ccgcgccggt gcctcgcgcg caggcggcca ggaggagggc ggtgatgggg
     3301 aggatgccga gtgctcggcg catccgggcc atggcaggta ccaatccttt cgttcggtgc
     3361 ggtcgtgacg tgtctcgtgt gttcgtcacg cctccgggcc gcaccgcccg tgccgggcac
     3421 gccccttccc cgggatcgcg aaggaaactt cacgcccgcc tttcgcgcgc ttcaccatgt
     3481 cgcccagtgg gcgatcgcga agcactcggg taggatgtcg gggatgatcg agacgctgct
     3541 gaccgtgacc ccggacgccc gccagaagat cgacggcgtt cgcaccttca acaacttccc
     3601 cgaggcggtc ctgcgcgtcc gtatcctggg caaggacggg ccgcgattcc gctacgagat
     3661 cgccctcgag gatccgcgcg accgaaccgc ggaggacctc tcactggacg tcgacggcct
     3721 gcaggtcgtg gtggacgcga ccagcgcggc cgatctcgcc ggtgcgagca tcaccctgga
     3781 cgacgcgatc accggcggcg cgctgcgcgt ggagaacccg aacgaggggt ggggcgaccc
     3841 ggtggcgcag gcggtccagg acgtcatcga ccgccagatc aaccccggcg tcgggtcaca
     3901 cggcggcatg gtgtcgctcg tcgaggtgcg cgaggggacg gcctaccttc ggttcggcgg
     3961 cggttgccag ggttgtgcgg ccgtcaacgt caccctgcgc cagggtgtcg agcaggcgat
     4021 cctcggctcc gtgccgcagg tgaccgccat cgcggacgtc accgatcacg gggcaggcga
     4081 aaacccctac taccgccacg ggcactaggg gatctcgcgc tcaggtgcgg cccctggggg
     4141 gcctgttacg cctagcggcg gagcgggaag tcgccgccgc cggtaaccgc catcacggtc
     4201 gagacgaggc tgaggatgct cagcgccgcc gccgtgccgg cgatcatcgc ggatcggatg
     4261 attcgcttgg tgcgcatcgg tcgggctcct tcgaggtgcg gccaagcggc cgtccccgcc
     4321 accctctcgc cgatccgccc atcggacaat gacccgtcgg tcctgcccgg actggcgccg
     4381 tggagtactt tcggcgggca cctcggtcaa gtgacgcttg ccatggcggt gtacagtgca
     4441 tggatgccgc gtacacgcac tgctgctcgc gccaccgaca aggatcgcga gctggcgcag
     4501 cgcatcggcg ttcggatccg cgaggcgcgg catcgtgccg ggctgaccca gcagcagctg
     4561 gccggcgagc ggtacacgaa ggcctacgtc agcgccctcg agaccgggat cgcgcgaccg
     4621 tcgatggtgg cgctcagcta cctgtcacga cggctcggct tgccggccag ccacttcatc
     4681 gacgaggcgc atccggcctg gacccggctc gaggtcgaca tgcgcctcgc ggcgggggag
     4741 tggacccagg ccgccgatgg ctaccgcgcg ctgctcgacg acgccaccga cgacatggtc
     4801 cgcgcggatc tgctgcgcgg cctggcggag gcgctggcca ggctcgaccg cggacgcgag
     4861 acgatcaccg ccgcctcgga gtctgcccgc ctgtacgcgg aggcgggtcg cgagagcgat
     4921 gccgcgttgg cgcgctactg gctcgcctac gggctgtacc agtcagacaa cgagccagat
     4981 gcgcgcagcc tcctcctctc cctgctcgag caggtgcgcg gcggccttgc ggtcgagcca
     5041 gacttcgaga tgcgcctgct gaccgcgctt gccgcggtcg aatcgcgctc ggggcagccg
     5101 gcgcgctcgc tcacgtacct cgaggaggcc cgcgggctcg cctcggatct cgacgaccgc
     5161 aggcgggcgg cgttcctctt caacctcgcc atcagctatc gcgaggtcgg cgacttcgag
     5221 gcggcgatcc gtgccggcac gcagggcatg gccctgtacc gggcggccgg cgccgaattc
     5281 gagtcggcgc gcatcgaaaa cgacctggcg ctggcgtacc tggccaccgg ccaggtgagc
     5341 cgggcccgcg atcttgcggc cgacgcgcgt cgccagatcg aggagagcgg cgacgagcgg
     5401 tggctcgcgg cggtcatcga caccgaggcg caggtcgcgc tggcggacgg tgatgccgag
     5461 ggcgcgctgc gactctcggc cctggcccgc gagcacgcca cccgcagcgg caacgagctg
     5521 gcacgtctcg ccgccgtcat caccgaggct cgggcgctgc gccgcagcgg acgcgacccg
     5581 gaagccgagg atcgcttccg caccgccgcc gagatcgcgc gaaccagccg cgtgccatcg
     5641 cgcctgaagg acgtgctgcg cgagtgggcc gacctccggg cggaggccgg cgaccatcgc
     5701 ggcgcgtacg agctcaccag cgaggcgctg gctgtcaatt aggtgttgac aggggcggtg
     5761 gcggaccgta cgctgccgcc gtgacgagtc ggatgggcac gccgacgtcg ccagtgggat
     5821 cggagatggg gcacgtctcc gatccctctc tgtgtcgctg gcctcgctgg cgagtcagct
     5881 gctgggttgg gcggcgcccg cgagcgtgaa ggtgccgatc gcgatgggtg cctccgtcgg
     5941 ctgcgcgaag atccgcatca cgtagtcacc cggcccgaag tcggcgatga gtgcatcggc
     6001 cggcacgacg aaggcgatca gggccgcacc atccggaagc gtctgtggcg acgccggctg
     6061 gaccgtcgcc gggtcgctgc cggtgcgcag gacctcgacg tagatggtcg gcgggggcgg
     6121 gagcgctggg cgcaccgagt aggcgaagtc gtcgccggcg ccgaaggtcg tggtcgcgga
     6181 gtctgcggcg accgcgcccg tcacggcgtc gatggcggtg ccgaaggcga tggcgggtgc
     6241 cgaaggcccg gcagtggccg atggcgatgc gctctgcagg gcaaccgcct cgtctccggc
     6301 ggtgccgatg agccgcgctc cgaacgcgat ggctacgagc agcagcgcga ccgcggccca
     6361 gccgaccagg cctcgcgcgg cggggggaat gctcagccgg cgagcgtctt cagggcgccg
     6421 ggccatcggc tgctcgcgcg gccggcccgt cagtcgttgc cgcactcgcg ccgtccctcc
     6481 gccggtcgtc gcgcccgggt ccccatctgc cgttccatcc tagcgatcgc cgtcggggta
     6541 ggatgggatc gatggcacgc cgtctcttca tcctcgacga cccggaccga ttcgtcgccg
     6601 gcacggtcgg tgagcccggc gatcgggcgt tctacctcca ggcccgcaag gggagcacgg
     6661 tggtcagcgt cggcctggag aaggcccagg tggccgccct cgccgaccgt ctcgatgacc
     6721 tcctcgatgc cgtcgaggca ccgccgcgcc cgaccgccac ggacgagggc gatctggatc
     6781 gaccgatcgt cgagatgttc cgcgtcggcg cgatggcgct cgcctgggac gcggccagcg
     6841 agtcggtggt gatcgaggcc cagaccccga ccgaggacgg cgagtacgcc gagctgcccg
     6901 acgatgccga ggacggaccg gacctgctgc gcgtgcgcat cgatcccgga acggcgcgcg
     6961 cgttcgtgcg ccgcgctgag gccttggtgt ccgccgggcg gcccctttgt ccgttctgcg
     7021 ggcagcccct ggacccccag ggccacttct gcgcgcgctc gaacggtcac ctcaactgac
     7081 ctcggtggcc gagccgctgg atccgccact gcgcgtcgac tggatcgagg cggcggagct
     7141 cggtgacggc cgaccgggtc ggctgggcct gacgttcctc cccggcaagc gcggcgcctc
     7201 gctccggtac ccggggctcg tgtaccgccg cgagctcgcg tccgacgtcc ggacgttgct
     7261 cgaggatggc gtcacgcggc tcgtgctcct cgtcgaggac accgagctgg agcgctgggg
     7321 cgacccggac atcgtccagg ccgcggcggc cgcgggcctc gacgtgcggc gcttccccat
     7381 gccggacggg gagccgccga cctcgatcga ggagatggac gcgatcctcg cggcgctgga
     7441 cgaggctcga gtcgacggcg acgcggcggt ggcctgcatg ggcggggtcg ggcggaccgg
     7501 gacggtcgcg gcatgcgcgc tggtccgggc cggggtcccg ccgcgcgacg cgatcgagcg
     7561 ggtgcgcgac gtccggcatc cggaggcggt cgagacgcgc agccaggagc ggttcgtcgc
     7621 cgcctacgcc gcgcgacggc acgcctgaga cgatcctgag acgcggcatg cggcccgagc
     7681 gcgttctggg catcgcgctc gtgctgatcg tggtggccgc ggccgcggtg ggcgtgtgga
     7741 tcgcgctcgg cagcggaacc aacgcggtgc ccgccgacgg cggcacagga ccgatgctgg
     7801 cgtcgccacc cgccggcgag cccgcacgga tcgagcgtgt catcgatggc gacacggtcg
     7861 tggtcgacct cgacggccgg cgggagcgcg tccgctatat cggcgtggat gcgcccgagc
     7921 tcgccaacgc ggaagccggg atcgctgccg aatgctgggg ccaggaggcg cgccaggcga
     7981 acgagcggct ggtcgacgac gccgaggtgc tgctggagcg cgacgtgtcc gaccgcgacc
     8041 gcttcggccg gctgctgcgc cacgtctggg tctcggagga tggagggtgg cggctcgcgg
     8101 ccgaggagct cgtggccatc ggcgcggtcg aggcgcgggc ctatccgccg gacaccgctc
     8161 gagacggcga gctcgaccgg gccgaacggg ccgcgcgccg ggacggccgc ggactgtggg
     8221 gcggctgcta gtccaccagg ccttcggcaa gctcgacgaa ctggcacaac gcgttggcga
     8281 gccgcatctg ctcgggagtt cgagctcgga cgcgcagcag ctccggggac gcgacgacca
     8341 ccgcgacgca gcgtgcccga gacgtcgcca cgttcagacg gttgcgcgag tacaggaagt
     8401 ccatgccccg cggggcgagc tccggcgagg aggtcgtcat cgagtagatg ctgatcggcg
     8461 cctgctggcc ctggaacttg tcgacggttc cgaccctggc gtcggggagg agccgctgga
     8521 tctcgccgac ctgggcgttg tacggggcga cgatgaggac gtcgttccac cggagcggat
     8581 gctgctcgcc gtccgcatcg gtccacgccg aaccgctctg gacgagggcg cggacgaggt
     8641 cggccacctg ctgcgcctcc tcgggtgacg cgttgtcggc acccgcgtgc tcggctgtga
     8701 tcaggcgcac gcccgtaccg cccacggggg ccggcgcccg cagccactgg gtggcgagct
     8761 gtggccgcga ctcgaggcgc cgctcgtaga aggcgctcga ggtgaaggcc gtgatgtcgg
     8821 ggtgcatgcg ccaggtgtcc tcgaggaaga ggccgaggtg gggcggcatg gtctgctgct
     8881 cgccgaggag gtgggcgagc gcggaacggt ccgcaccggg aggatgggag ccctgcagcg
     8941 gctggtcgag ctgttgcgga tcgcccagca gcaccagcga gtcggccgcc ccgcccatcg
     9001 ccaccacgtt cgcgagggag atctgccctg cctcgtcgac gaacagcacg tcgagcagcc
     9061 cgtcgctcgt ctccgggacc cacagccacg acgtccccgc caccaggttc agctccccat
     9121 ccgcgagacc ctgccggatg gggccggctt cgccttcgac gcggacgcgt gggtcgtcca
     9181 gcccggtgac tccatcgctc accttctggg ctgcgcgaac gtcgacaccg cccacggcgt
     9241 cggcggcgca cagcacctcc tccaggaagt gcgtgatcac cttgtggctc gtggcgctga
     9301 tgccgacgcg ctttccggcc cgcagcagcg caacgatcat ccgcgccccg gtgaacgtct
     9361 tccccgagcc tggcggtccc tggatggcca gggtggagtg gtcgagggcg agcgccagtc
     9421 ggcacgccgc ctcgaggtca ctctcgccgg gcagcttcag ccccgctccc gccgcctggc
     9481 cgcagcgggg tggccggcgc agcagcaggt cccgggctgc cctcgccgga ccgtcggcgt
     9541 cgacgccgtg gtcggcgacc cactcgccca ggcgcagcaa cgcggcgcga tggtcgttgt
     9601 cgccgatgac gttgagcggc acgatcgccg tcggatgctg cggctccacg tcgacgttcc
     9661 aggtcaggac gatcgactcg cggcggtcgt cgatctcctt gagcttgccc ttgatgcgcc
     9721 agcgtttcat ctcgtcgggg aatacctgct ggagcgccgg gtcgtacagc tcggaccggt
     9781 cgccgatgtc gtgctcctgc ggtggcatgg cgtaccgcca gtcctgccgc cacttcgact
     9841 tcggcgtggg ccgatacggc tcgccgtcgg gaccgatcag ccgcagccgc cccagcgcga
     9901 cgtcgtcggt gacgagctcg tccgacgtca taccgatgcg gtggaagaac tcccaatagg
     9961 tcgccttcat ctcccggcgg tgccaggaga gcagctgcgc cagcagccac cgggcctgct
    10021 gctccgctga gcgttcggcc tcgtccgccg gcacgccatc ggtcaggcgt gcgccgactt
    10081 cctcgacgcg cgccgcgacc tcggacagct tctcggccgg ctcgggagac tgcggcacgg
    10141 gtcgcggcac gggcgcgccg gtcatcgtct cgagctcccg gcggcgcgcc tccagccagt
    10201 cacgcaggcg cagcgtcgag accacgtcgt cgcggttgta ggcggcgatc tcgtccagga
    10261 tggccgacga cggccgctcg gtatcggcca gctggagcca ctcctcgaac gccacgatgc
    10321 tcgaggtcgc gtccttgagc gggatggcgc gctcgaagcc gtacagcggc tccagccgct
    10381 tgatcgagta gctctcgacg gaggcgcgaa cgccctggcg gacggcgcgg aacaggtcca
    10441 cgagcacgcc gccgcgcagc agccgatcga ccgcctcctc ccgggtcgcg tggcggccca
    10501 tcagccgctt gagggcggtc ggctcgtagg cggcgtagtg gtagacgtgc atgttcgggt
    10561 agcgctcgag gcgttcggtg atgaggtcca tcacccgctc gaaggcggcc ttctcaccgg
    10621 caaggctgaa ctcctccggc cggtccgggt cgcgtgacca gatggccgtg aaagcgccgt
    10681 cgacatcgag caggccgaag aggtagtcga agctgtcacc gaacgcgtac gggtcgccct
    10741 cgagatccag gaagagatcc cccggatcag gctccggcag cacggcgagg ccgcgctccg
    10801 cctcaacggg cgtgatcagc tcgtacagca gctgcggggc gaccgcgcgc ccgcgaacct
    10861 ggatcgcggc ctggcgctgg atgcggtcca gggccgcggc gctcgcgccg tccagctcgg
    10921 gcagcgggat gggcgcgttc gccagcttca cgacggtgtc gatcccgccg gcggtcagcg
    10981 cgcgtcgttg gccggcactg atccccgcca ccaggctgag gtggtcgtcc tcacgccgcc
    11041 ggtcggcgca gacctgccac cagcggcaca cgtcgcaatg atcgaccggc tcgggatacg
    11101 tcgttccggg cggcagctcg aatgccgcct cgaagcggcg cctggcggcc cggtagtagg
    11161 ccatgtagtc atccacccgc agctcgaccg ggtcgcgtcc gccgccgagg acgacgcgga
    11221 ggcgttccgg ctgcacgccc tggatggcgc tcagctgctc gacgtaggag cagatctgca
    11281 gcacggcgct ggccttgacg tgtcgcgcca gcttggtgtc cgccacctcg tagtggtacg
    11341 ggccccagac cgatgcgcga tcgcggtcat cagcttcaac gcgcaggagg aagtccgcgt
    11401 agccgagcca gcgtccgtcg aagaaggtcg cctggtagat gacgtcggca ccggatgcca
    11461 tggcgtcgac cgtcgcgcgt gcctggcgct cgagccggtc ccggccctcg ccggcgtcct
    11521 tctcgatccg gacgacccgg cggccgtcgg cctcgagctg ctcgaggtag cgcgcttcgt
    11581 gcagctcccc gtgacggcgg atgaggtcca gctcccggtc cacgcgatgt ggcttgagct
    11641 cgggatggcc cagcaccgag cgctccaggg cggtgaggtg ctcgcaggcc aggtagccga
    11701 cgaggtcggt agcggagaag acgggcgtgc cgtcgatgag ctgcacggtg atctcaggct
    11761 cgcacgggca cgcgacacct cgtcggtcgc gatcgaccag cgccggtcac ggggcggcgc
    11821 tcgcgctggg ctgcggcgtc tcgtcgggga cgcgcaccac cgccagcgcc cagtcgcagt
    11881 cgctggtgat ggtcacgaag gcgaggcccg tgatgccgag ggccgcgaac gattgcggct
    11941 gcagggttcc gggcacgacg tcgttcccga ccgtggcgcg gatggcgggc acgtcggtgg
    12001 agtccgtatc ggtcatggcg accgagaact ggcatcggcg gccctcgggg tcgacggcga
    12061 tccagcgcac ggcgtgggca ccgtcgccca ggtcgatcgg tccgtactcc gcgtcaccga
    12121 gctcggcgta cgaagcgagc accgggctgc cctcgacggc acggcccgtc gcggcgaggt
    12181 agggctccgg gccgagaccg ctggcgatcc agccctcgct gaaggccggc tcggcgggat
    12241 cgacgtccgt gacgaggaac cagccctggc cgtcgaccag gaccggtccc atgacgacgc
    12301 cgacctccga gccgagcggc atgaggccgg tcagcacgac ggtggacgtc cccggacgct
    12361 gtcgcacgcg cagtccgtcg atggttgtcg tcgcgacctc gccacccggg atgcgcggca
    12421 tggcgccgac atccggtgtc ggctccgggg tgggcgtcgg gaccggcgtg ggcgtcggtg
    12481 acggcgaggg cgtcggcgtg ggagtggggg agggcgtggg cgtcggggtc ggcaggggtg
    12541 cgaaggtgca cgccgtgagc agcacgaggg ggcacaggct ggcagcgcgc gctcgggtcc
    12601 ccaccgcgca agggtagcgg gtcagcggtc caggcgcggg acctcggacg ccaggaggtc
    12661 ggcgatctcg cgcgcgttcg tggtcccgta gttggcatag cagttgttca tgaggacgtg
    12721 cacttccggc acctgcttcg ccgtctcgcg gatgcgcggc acccattcgc ccagctcctc
    12781 aggcccgtac aggtagcgga accgctcgac gaccgggatg ccggacttct cccatgtttc
    12841 ggtgcgccgc ccgtggaagc ggacgacagc gagctttggt gacgtgacga ccgtcatcgg
    12901 tggcagcgag gacttggtcc cgggtggcgc gtcgaccatc acgaagggga tccgctcgtc
    12961 ctcgaagaag cggatcgtcc gctcgaggtt cttctcgttg aaccagctcg cgtgccgcag
    13021 ctcgacggcg aacggcgtgt cgcccagccg caccttcgcg cgccggatct cctcgcgtga
    13081 ctcggaggtg gggaagaacc agcgcgggta ctggaggaag
//
LOCUS       DASTYP010000255         9550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_507935, whole genome shotgun sequence.
ACCESSION   DASTYP010000255 DASTYP010000000
VERSION     DASTYP010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507935"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFV59_11030"
     CDS             <1..232
                     /locus_tag="VFV59_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326405.1"
                     /translation="TAQSKLDGLREHYAGTVDEGAQAYFRVHAVETDEPAMLAERIDR
                     LTDEEFARTRTACAVFSAAAWGALDGVFYLDA"
     gene            complement(320..1537)
                     /gene="chrA"
                     /locus_tag="VFV59_11035"
     CDS             complement(320..1537)
                     /gene="chrA"
                     /locus_tag="VFV59_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEU5326406.1"
                     /translation="MNAENGTAGPIHTGTPLEVLRVALRLGLTSFGGPIAHLGYFRRE
                     YVERRRWIDEHTFADLVALTQSLPGPASSQLGIAIGTRRAGMLGGLAAWLGFTLPSAV
                     ALVLFGLLATSPDVADAGWIHGLKLAAVAVVAQAVSAMSRSLTPDWPRRALAAAATAV
                     ALLWPSPLTQVAIIGGGAVIGRLMLAPPSARPTGPEPSPVPRRIGLMALVAFFVLLAG
                     LPVLRLAVDAQGLHLFEGFYRAGALVFGGGHVVLPLLHSTVVDPGWVSNDAFLAGYGA
                     AQAVPGPLFTFSAYLGTVSSVAPNGIVGAGIALAAIFLPAFLLVFGTLPFWDALRTSD
                     GFRRALSGTNAAVVGILLAALVTPVWTGAVTGPLDVVIVAAGLAMLLTGRVSPIIVVA
                     LSAVLAQVIAASG"
     gene            1678..2148
                     /locus_tag="VFV59_11040"
     CDS             1678..2148
                     /locus_tag="VFV59_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326407.1"
                     /translation="MALLAAAALAVTATACGGGGGSPSAAASSGGGGGDATSVDITLQ
                     EWAVVVGSESAPAGQVTFNITNDGPEDVHEFVIFRTDLDPGSLPTDEHGAVDEEGEGL
                     EVIDEVEDVAVGATEQLTVDMDPGSYVLVCNIYSEDEDEAHYQMGMRSPFEVTD"
     gene            2174..3337
                     /gene="solA"
                     /locus_tag="VFV59_11045"
     CDS             2174..3337
                     /gene="solA"
                     /locus_tag="VFV59_11045"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013878929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEU5326408.1"
                     /translation="MTERRDRFDAIVIGLGGMGAAAAFHLARRGQRVLGLEQYDLLHD
                     RGSSHGLTRIIRLAYHEHPSYVPLLRRAYDLWHDLEAEASERLLVTTGSVEGGPADGP
                     MFRGALEAAELHDIPHEVLDGAEVRRRYPAYAGFDEAMRVVWQPDGGFLLAERTILAH
                     VNGALRRGADLRFREPVLRWGATDGGGVSVETERGRYEADRLVIAAGPWASRLVPRLA
                     TLAVPERQVLAWLQPMRPELFTPDRFPVFILDVPEGSYYGFPVHDVPGFKIGWYHHFR
                     EPIDPDAPDRAARPDDEAALRSFAERYFPDGAGPTVMLKACIFTNSPDEHFIIDRLPD
                     VPQVAIAAGFSGHGYKFCSVVGEILADLSMHDETGHDIGLFRLGRFDGANLGS"
     gene            3499..3843
                     /locus_tag="VFV59_11050"
     CDS             3499..3843
                     /locus_tag="VFV59_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326409.1"
                     /translation="MTQRSPVPDATAKSAAHGEPPPADVAGFIRFCHRRRGATWPELY
                     DEMCAVAARREYRGWDHGALAERGLTFSLLEMPRLANWSRAVLAGMAEERETDGHRGA
                     TTARLEARPVLA"
     gene            complement(3917..4216)
                     /locus_tag="VFV59_11055"
     CDS             complement(3917..4216)
                     /locus_tag="VFV59_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326410.1"
                     /translation="MADEPIFTKVQLQRTQMDVDRELLRDEDLLNEEHLRRMDQVLLR
                     RNLDHARMFSRKLSLELQQYVHLYTPEANELRRRITKYMVMERMIEREMARRHTY"
     gene            complement(4283..4447)
                     /locus_tag="VFV59_11060"
     CDS             complement(4283..4447)
                     /locus_tag="VFV59_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326411.1"
                     /translation="MDRGRIRRRLWAPLDPAWDSDTIGRFVFGPPTPAHLLAGLLIGL
                     LIAILFVVVG"
     gene            4622..5488
                     /locus_tag="VFV59_11065"
     CDS             4622..5488
                     /locus_tag="VFV59_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU5326412.1"
                     /translation="MTDQPQVLLDGFGMGESPRWHDGRLWFSDWGTDEIVAVDLDGRR
                     EVIGPGGGGAGWAVGWLPAGRMLVTGPQLVRQEADGSRVRHADLSSVLPYGCSELTVD
                     GRGNVYVNSINFDFADFGGVMARGEAPGKIALIAHDGAVREVTDGLAFPNGMVVTPDN
                     ATLIVAESFARRLTAFDIAADGSLSNRRVWADVTGDGICLDAEGAVWTSDVQPGDRGV
                     VRRVAEGGEVLDEIELDRPCYACMLGGDDGRTLFMVAAKWFGPDRMDELIAAGTGQVL
                     SVRVDVPHAGWP"
     gene            5535..5610
                     /locus_tag="VFV59_11070"
     tRNA            5535..5610
                     /locus_tag="VFV59_11070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5568..5570,aa:Arg,seq:tct)
     gene            complement(5614..6828)
                     /locus_tag="VFV59_11075"
     CDS             complement(5614..6828)
                     /locus_tag="VFV59_11075"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5326413.1"
                     /translation="MRVALATVGTMGDVLPFVLLARELATRGHEVTAITWPVHATAFD
                     LPGVRVEPAGPHADPERIAAVAAEAASRGPMDQVAVLRDFHLADGEAHERRLRELLPG
                     HDLVVLHGIHALAHAAALDENLPWVTAVFDPVLLPTATGPPPGMPNLGPLNRALWWML
                     DRALARTSRPLDGVLERSGNAQRGLPLFRARSPRLHLIGCSPGIMRIPTDLPPNTRIT
                     GAWLDHDAPSPLPDGVEAFLADGAPPIVMTFGSMHGIDATVLDAAIEGILESGRRVIL
                     QGRTEVTSPGLLSIGAVDHRSLFPRAALVVHHGGAGTTNAACAAGVPSLVVPHVGDQR
                     YWADRLHRLGVAPAPQPVSKLTADGLVSAALAAASDHDMHGRARALGQRVRAEDGVTT
                     AADAIEGVAPAG"
     gene            complement(6834..7730)
                     /locus_tag="VFV59_11080"
     CDS             complement(6834..7730)
                     /locus_tag="VFV59_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEU5326414.1"
                     /translation="MIEASVSLGRPLDLVTTLAPLRHGAGDRTVLLAAAEAWLAWREP
                     NGPAAIRLRVTTQRSLHATAWGDGADAALARVPALIGEADDPDALVACHPIVRELQRR
                     HSGLRLPRSGRVFDALVPSILEQKVTGTEAFRAYAALQRRYAERAPGPMRQLRLPVTP
                     EVLAALPYHAFHPLGVERRRADVIRRAARRAGWLDASVDATQAARRLRSLPGVGPWTT
                     AEVVRSAFGDPDAVSIGDFHIPHSVAWALAGEPRGTDARMLELLEPYRGQRGRVQRLL
                     EVGRITAPRYGPRLAPRSIAAI"
     gene            complement(7727..8731)
                     /locus_tag="VFV59_11085"
     CDS             complement(7727..8731)
                     /locus_tag="VFV59_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326415.1"
                     /translation="MTRAAVAFPGRGAYTPSSLGSLAPDHPWVTRADDLRREAGLPTV
                     TELDGARSFDPSVHLRPTNAWPLTFLAGLLDAERIADDHEVVVVVASSSGWYTALAAA
                     GALPFDDAFRLVAAMAEAAEAPLPDGERPADLIYPLADATWMPDPELRAAVDGAVASS
                     DGRLELALDLGAFAILSGTTAAIEDAATDLPEREVAGRTYPLRLGGGDAWHTSLRTAA
                     VAQAIADLTGLTWQAPNVALVDGHGRRHTPFSSVPAVLAEHTLREQPVTTYDFAASFR
                     VALREYAPDVILLPGPGSSLGAACAQLVVREGYRGLRSRADLEAAQRSPNPILLSMRR
                     "
     gene            complement(8728..>9550)
                     /locus_tag="VFV59_11090"
     CDS             complement(8728..>9550)
                     /locus_tag="VFV59_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5326416.1"
                     /translation="EIQYLAYIHNALDQLRGEAASLQFFSSGQFRNPMVVRVAGLAYQ
                     KGFGGHFHNDNSIGALRDIPGLVLAVPSRGDDAARILRGAIAMAAEDGRIVVFLEPIA
                     LYHEKDLYADGDAGWLVDYPAPPSVLLPGEVGLHGATDTDADLLIVTYANGVRLSLQA
                     ARRLEENHDLRVRVLDLRWLNPLPFESIRSHAADIGNVLVVDEARATGGGVADAVIAD
                     LAERRVGRRLASVRAVDSFVPLGPATSAVLVGVDQIVASGVAAVGRRRSAQAARA"
BASE COUNT         1409 a         3295 c         3575 g         1271 t
ORIGIN      
        1 caccgcccag agcaagctcg acgggttgcg cgagcactac gccggaacgg tggacgaggg
       61 cgcgcaggcg tacttccgcg tccacgccgt ggagaccgac gagccggcga tgctcgcgga
      121 gcggatcgac cgactcaccg acgaggagtt cgccaggacg cgcacggcgt gcgccgtctt
      181 cagcgcggcg gcgtggggtg cactcgacgg agtcttctac ctcgacgcct gatcgatggc
      241 ggtcggcgtt cgggctcgcc aggtcgagaa ggacctgcgc gtgccgacgg aggccgccga
      301 cgagggctgc gcgggctgct cagccagagg cggcaatgac ctgggcgagc acggctgaca
      361 gtgcgaccac gatgatcggg gagacccgtc ccgtcagcag catcgcgagg ccggcggcga
      421 cgatcaccac gtcgagcggt cctgtcacgg cacccgtcca gactggcgtc acgagcgcgg
      481 ccagcaggat gccgaccacc gccgcgttgg tcccggagag cgcccggcgg aacccgtccg
      541 aggtgcgcag ggcgtcccag aatggcagcg tgccgaacac cagcaggaac gccggcagga
      601 agatggcggc cagcgcgatc ccggcgccga cgatcccatt cggcgccacc gacgacaccg
      661 tgcccaggta cgcggagaag gtgaacagcg gcccgggaac ggcctgcgcg gcaccgtagc
      721 ccgccagaaa ggcatcgttg ctgacccacc ccgggtcgac gaccgtcgag tggagcagcg
      781 gcagcacgac gtggccgccg ccgaacacga gggcgccggc gcggtagaag ccctcgaaca
      841 ggtggagccc ctgtgcgtcg acggcgagcc gcagcaccgg cagcccggcc aggaggacga
      901 agaacgcgac gagcgccatc aggccgatgc gtcgtgggac cgggcttggc tccgggccag
      961 tcggtcgggc ggacggcggc gccagcatca gccggccgat gacggcgcct cccccgatga
     1021 tcgcgacctg ggtgagcggc gagggccaca ggagcgcaac ggcggtcgcg gcggccgcca
     1081 gggctcggcg cggccagtcg ggcgtgaggc tgcgcgacat ggcggacacg gcctgcgcga
     1141 cgacggccac cgccgcgagc ttgaggccgt ggatccagcc cgcatcggcg acgtcgggcg
     1201 aggtggcgag caacccgaac aggacgaggg ccactgccga cggcagcgtg aacccaagcc
     1261 acgcggcgag gccgccgagc atgccggctc gccgcgtccc gatggcgatg ccgagctgcg
     1321 aactcgccgg acctgggagg gactgggtca gcgcgacgag atcggcgaac gtgtgctcgt
     1381 cgatccagcg gcgccgctcc acgtactcgc ggcggaagta gcccaggtgg gcgatcggtc
     1441 caccgaacga ggtgaggccg aggcggaggg cgacccgcag gacctcgagc ggcgtgccgg
     1501 tgtgtatggg cccagcggtc ccgttctcgg cgttcacgcg gcgcaatgct aggcgagagg
     1561 ccgctcggcc cagcgctcgt ccgtgacgga gccttgtgta ccggtcgttc acaggactac
     1621 tgttcccaga attaccaact caataccggg agggaactca tgctgcgtgg gaaacgattg
     1681 gccctgcttg cagcagccgc gctcgccgtc acggcgacag cctgcggcgg aggtggcggc
     1741 tcgccctcgg cggccgcgtc gtcgggcggc ggtggcgggg acgccacgag cgttgacatc
     1801 accctccagg aatgggccgt cgtcgtcggc tccgagtccg cgcccgcggg ccaggtgacc
     1861 ttcaacatca ccaacgacgg gcccgaggac gtccacgagt tcgtgatctt cagaacggac
     1921 ctcgatccgg gttcccttcc caccgacgag cacggtgcgg tcgacgagga gggggagggc
     1981 ctggaggtga tcgacgaggt cgaggacgtc gcggtcggcg ccacggagca gctgacggtc
     2041 gacatggatc cgggcagcta cgtgctcgtg tgcaacatct actcggagga cgaggacgaa
     2101 gcgcactacc agatggggat gcgcagtccc ttcgaggtga ccgactgaga acgtcggacc
     2161 gtagactccg ccgatgaccg agcgcaggga ccgcttcgac gccatcgtca tcggcctggg
     2221 cgggatgggg gccgctgccg ccttccacct cgcccggcgc ggccagcgtg tgctcggcct
     2281 cgagcagtac gacctgctcc acgaccgcgg ctcctcgcac ggcctgaccc gcatcatccg
     2341 gctggcttac cacgagcacc cgtcgtacgt gccgctgctg cgtcgtgcgt acgacctctg
     2401 gcacgacctg gaggccgaag ccagcgagcg cctgctggtc accaccggca gcgtcgaggg
     2461 tggcccggcc gacggaccga tgttccgcgg ggcgctggag gcggccgagc tccacgacat
     2521 cccgcacgag gtcctcgacg gggccgaggt gcgccggcgc tatccggcgt acgccggctt
     2581 cgacgaggcg atgcgggtgg tgtggcagcc cgacggcgga ttcctcctgg ccgagcggac
     2641 gatcctggcc cacgtcaacg gcgccctccg gcgcggcgcc gacctccgct tccgcgagcc
     2701 ggtgctgcgc tggggagcga ccgatggcgg cggcgtatcg gtcgagaccg aacggggccg
     2761 ctacgaggcg gatcggctgg tcatcgcggc cggaccctgg gcatcacgcc tggtgccgcg
     2821 gctggccact ctggccgtcc cggagcgaca ggtgctcgcc tggcttcagc cgatgcggcc
     2881 ggaactgttc acgccggatc ggttcccggt cttcatcctg gacgtgcccg agggcagcta
     2941 ctacggcttc ccggtgcacg atgtcccggg cttcaagatc ggctggtacc accacttccg
     3001 cgagccgatc gaccccgatg cgcccgaccg cgccgcccgt cccgatgacg aggcggcgct
     3061 gcgctccttc gccgagcgct acttccccga cggcgccgga ccgacggtca tgctcaaggc
     3121 ctgcatcttc accaactcgc cggacgagca cttcatcatc gatcggctgc ctgatgtccc
     3181 gcaggtcgcc atcgcggccg ggttcagcgg ccacggctac aagttctgca gcgtcgtcgg
     3241 cgagatcctg gccgacctgt cgatgcatga cgagaccggc cacgacatcg gtctgttccg
     3301 tctcggacgg ttcgacggag ccaatctggg cagctgagcg tggtgtgcag agaatgtgga
     3361 caacttggtg gatgaaccgg tttcggtggc cgcgaacgca ggcctacgat cccggccggt
     3421 catgcatcct ttcggccggc gcatgaccca ccgtcatcat ccaattcacg tgtccaaact
     3481 cctggaggga gccatcgaat gacccagcgg tcgccggtgc ccgacgccac cgcgaagtcc
     3541 gcagcccacg gcgagccacc gcccgccgac gttgccggct tcatccgctt ctgccaccgc
     3601 cgccgtggag cgacctggcc cgagctgtac gacgagatgt gcgccgttgc cgcgcgtcgc
     3661 gagtaccggg gctgggatca cggcgcactg gcggagcgcg gcctcacctt ctcactgctc
     3721 gagatgccac ggctcgccaa ttggagccgt gccgtgctgg ccgggatggc cgaggagcgg
     3781 gagacggacg gccaccgcgg ggccacgacc gcacgactcg aggcgcggcc ggtgctcgcc
     3841 tgagccgcgt ccgcccgcac atggcatcgc cccggtccag ctcggaggac cggggcgtct
     3901 tcatgtccgg cgcggatcag taggtgtggc ggcgcgccat ctcccgctcg atcatgcgct
     3961 ccatcaccat gtacttggtg atgcggcgtc gcagctcgtt ggcctcgggc gtgtagagat
     4021 ggacgtactg ctggagctcg aggctcagct tgcggctgaa catgcgggcg tggtcgaggt
     4081 tgcgccgcag caggacctgg tccatgcggc ggaggtgctc ctcgttgagg aggtcctcgt
     4141 cgcgcagcag ctcgcgatcg acgtccatct gcgtgcgctg cagctggacc ttggtgaaga
     4201 tcggttcgtc agccatcgcc cgtgagtcta gcgcgccgga cctgaggcgc gtccgacgcc
     4261 gtcctcggcg gttctcggcg ggtcagccca cgacgacgaa caggatggcg atcagcaagc
     4321 cgatcagcag tccggcgagc aggtgtgccg gcgtcggcgg cccgaagacg aagcgaccga
     4381 tcgtgtcgct gtcccaggcc gggtcgagcg gcgcccacag tctccgtcgg atgcgcccgc
     4441 gatccatcgg tgtctcccct cggcgtcggc gaatcgtcga atcggggacg tggctgggcg
     4501 agtgccgaag gtcccgaagc gcccgggagc gcgggttcgg gggcctgctg cgacaccgtc
     4561 tgcggtgagt tttcgcctcc gccgcggtcg aatcggtgaa ggcagcgatg agaggaacac
     4621 gatgacggat cagccgcagg ttctgctcga cggattcggc atgggcgagt cgccgcgatg
     4681 gcacgacgga cggctgtggt tctccgactg gggcaccgac gagatcgtgg ccgtcgatct
     4741 cgacggccgt cgcgaggtca tcggacctgg cggcggggga gccggctggg cggtcggctg
     4801 gcttccggct ggtcggatgc tggtcaccgg gccgcagctg gtgcgccagg aggccgatgg
     4861 gtcccgggtg cggcacgccg atctcagctc cgtcctcccg tacgggtgca gcgagctgac
     4921 cgtggacggc cgcggcaacg tctacgtcaa ctccatcaac ttcgacttcg cggacttcgg
     4981 cggcgtcatg gcccgcggcg aggcgcccgg gaagatcgcc ctgatcgctc acgacggtgc
     5041 ggtccgcgag gtcaccgatg gcctggcctt cccgaacggc atggtcgtca cgcccgacaa
     5101 cgcgacgctg atcgtggcgg agtcgttcgc caggcggctg acggcgttcg acatcgcggc
     5161 cgatggcagc ctgtcgaacc gccgcgtgtg ggcggacgtg accggcgacg gcatctgcct
     5221 cgatgccgag ggcgcggtgt ggaccagcga cgtccagccg ggagatcggg gcgtggtgcg
     5281 gcgggtcgcc gagggcggcg aggtgctcga cgagatcgag ctggatcggc cctgctacgc
     5341 gtgcatgctc ggcggcgacg acgggcggac gctgttcatg gtggcggcga agtggttcgg
     5401 gccggatcgc atggacgagc tgatcgcggc gggaacgggc caggtgctca gcgtccgtgt
     5461 cgatgtgccg cacgccgggt ggccgtgatc cgcgacgtcg gtggctgcag aacggacggt
     5521 atgcttgggc tgccgtcccc gtagctcagt ggacagagca gcggtcttct aaaccgccgg
     5581 tcgcaggttc gaatcctgcc ggggacgcca gcgtcagccg gcgggcgcga cgccctcgat
     5641 ggcgtcggcg gccgtggtca cgccatcctc cgcacggacc cgctgtccga gcgcgcgcgc
     5701 acgtccgtgc atgtcatggt ccgaggcggc cgccagggcg gccgagacga gcccatccgc
     5761 cgtcagcttc gacaccggct gcggcgccgg agccaccccc agccgatgca gccgatcggc
     5821 ccagtagcgc tggtcgccga catgcggcac gaccagtgag ggcacgccgg cggcacacgc
     5881 cgcgttcgtc gtcccggccc cgccgtggtg gaccacgagt gcggcgcgcg ggaagaggga
     5941 gcggtggtcg accgcgccga tgctcagcag tccgggcgac gtgacctcgg tccggccctg
     6001 gaggatgacg cggcggccgg actccaggat gccctcgatg gcggcgtcga ggaccgtggc
     6061 gtcgatgccg tgcatcgagc cgaaggtcat gacgatgggc ggcgcgccgt cggcaaggaa
     6121 cgcctcgacg ccatccggca gcggtgacgg cgcgtcgtgg tcgagccacg cgccggtgat
     6181 gcgcgtgttc ggcggcaggt cggtggggat ccgcatgatc cccggtgagc agccgatcag
     6241 gtgcaggcga ggcgatcggg cgcggaacag cggcagcccg cgctgcgcgt tgccggaccg
     6301 ctccaggaca ccgtccagtg gtcgcgacgt tctcgccagt gcgcggtcga gcatccacca
     6361 cagggcccgg ttcagcggcc cgaggttcgg catccctggt ggcgggcccg ttgcggtcgg
     6421 cagcagcacc gggtcgaaga ccgcggtgac ccacggcagg ttctcgtcga gcgcggccgc
     6481 gtgggccagc gcatggatgc cgtgcaggac gaccaggtcg tggcccggca gcagctcgcg
     6541 gagccggcgc tcgtgggcct cgccatccgc gaggtggaag tcccgcagca ccgcgacctg
     6601 gtccatcggt ccgcggctcg ccgcctcggc agcgacggcg gcgatgcgtt ccggatcggc
     6661 gtgggggccg gcgggctcga cccgtacgcc cggcaggtcg aaggcggtgg cgtgcacggg
     6721 ccaggtgatg gccgtgacct cgtgcccgcg ggtggccagc tcacgggcca gcagcacgaa
     6781 cggcaggacg tcccccatgg tgccgaccgt ggccagcgcg acccgcaccg gcgtcagatg
     6841 gccgcgatcg accgcggggc gagccgcggg ccgtagcggg gcgcggtgat gcgtccgacc
     6901 tcgagcaggc gctgcacgcg gccgcgctgg ccccggtacg gctccaacag ctcgagcatg
     6961 cgggcgtccg tcccgcgcgg ctcgccggcc agggcccagg ccacgctgtg cggaatgtgg
     7021 aagtcgccga tgctcaccgc gtcgggatcg ccgaacgcgg aacggacgac ctcggccgtc
     7081 gtccacggcc cgacgccggg aagcgaccgg aggcgtcggg cggcctgcgt ggcgtccacg
     7141 gacgcatcca gccagccggc gcgccgtgcg gcgcggcgga tgacgtcggc acgacgccgc
     7201 tcgacgccga gcgggtggaa ggcgtggtag gggagtgccg cgaggacctc cggcgtgacg
     7261 ggcaggcgaa gctggcgcat cggtcccggg gcgcgttcgg cgtaccgacg ctggagcgcc
     7321 gcgtaggcgc ggaacgcctc ggtgccggtc accttctgct ccaggatgct tggcaccagc
     7381 gcgtcgaaca cccgtccgga gcgggggagg cgcagcccgg agtggcggcg ctgcagctcg
     7441 cggacgatcg ggtggcacgc gaccagcgcg tcggggtcgt cggcctcccc gatgagcgcc
     7501 gggacgcggg ccagcgcggc atcggctccg tcgccccagg cggtggcatg cagcgatcgc
     7561 tgcgtcgtca cccgcagccg gatcgctgcc gggccgttcg gctcgcgcca ggcaagccag
     7621 gcctcggcgg ccgccaggag cacggtacgg tcaccggcgc catggcggag cggagcgagg
     7681 gtggtgacga ggtcgagcgg tcgaccgagg gagaccgatg cctcgatcat cggcgcatcg
     7741 acagcaggat cgggttcggg ctgcgctgcg cggcttccag gtcggcacga ctccgcaggc
     7801 cgcggtagcc ctcgcggacc acgagctgcg cgcacgcggc cccgagggag cttcccggtc
     7861 cgggcaggag gatgacgtcc ggcgcgtact cgcgcagcgc gacgcggaac gacgccgcga
     7921 agtcgtaggt cgtgaccggc tgctcgcgca gggtgtgctc ggcgaggacg gcagggacgc
     7981 tcgagaacgg cgtgtgccgc cggccgtgtc cgtccacgag cgcgacgttc ggcgcctgcc
     8041 acgtgagccc ggtgaggtcg gcgatcgcct gcgccaccgc cgccgtccgc agcgaggtgt
     8101 gccacgcgtc gccgccgccc aggcgcagcg gataggtccg gcccgccacc tcgcgctccg
     8161 ggagatcggt tgcggcgtcc tcgatggcgg ccgtcgtgcc ggagaggatc gcgaaggcac
     8221 ccaggtccag cgcgagctcg agacggccgt cggaggaggc caccgcgccg tccacggccg
     8281 cgcgcagctc cgggtctggc atccaggtcg catcggcgag gggatagatc aggtccgcgg
     8341 gacgctcgcc atccggcagt ggcgcttccg cggcttccgc catggccgcg accagccgga
     8401 atgcgtcgtc gaacggcagc gcgccggccg ccgcgagcgc ggtgtaccag ccgctggagc
     8461 tggcaaccac gaccaccacc tcgtggtcgt ccgcgatgcg ctcggcgtcc agcagcccgg
     8521 ccaggaacgt cagcggccac gcgttcgtcg gccgcaggtg gacggacgga tcgaagcttc
     8581 gggcgccgtc gagctcggtg acggtcggaa ggccggcctc gcgccgaagg tcatcggcgc
     8641 gggtgaccca cggatggtcc ggtgccagcg agccgagcga gctgggggtg taggcgcccc
     8701 gacccgggaa cgcgacagcc gcgcgcgtca tgcgcgagcc gcctgcgcgg accgccggcg
     8761 gccgacggcg gcgaccccgg acgccacgat ctggtcgacc ccgaccagca ccgccgacgt
     8821 ggcaggcccc agcgggacga acgagtcgac ggcacgcacc gacgccagcc ggcgcccgac
     8881 gcggcgctcc gccaggtcgg cgatgaccgc gtcggcgacc ccaccaccgg tcgcccgtgc
     8941 ctcgtcgacg accagcacgt tgccgatgtc cgcggcatgg ctgcggatcg actcgaacgg
     9001 gagcgggttg agccatcgca ggtccagcac gcggacccgc aggtcgtggt tctcctcgag
     9061 acggcgcgcc gcctggaggc tcagccgcac gccgttggcg tacgtcacga tcaggaggtc
     9121 cgcatcggta tcggtcgcac catgcaggcc gacctcgccg ggcaggagca ccgatggcgg
     9181 cgccggatag tcgaccagcc agcccgcgtc accatcggcg tacaggtcct tctcgtggta
     9241 gagggcgatc ggctcgagga acacgacgat ccgtccgtcc tcggccgcca tcgcgatcgc
     9301 gccgcgcagt atccgcgccg cgtcgtcgcc acgcgagggg acggcgagca ccaggccggg
     9361 gatgtcgcgc agcgcaccga tgctgttgtc gttgtggaag tgcccgccaa agcccttctg
     9421 gtacgccagg ccggcgaccc gcacgaccat cgggttgcgg aactggccgg atgagaagaa
     9481 ctgcagcgaa gcggcctcgc cccgcagctg gtcgagcgcg ttgtggatgt aggccaggta
     9541 ctggatctcc
//
LOCUS       DASTYP010000256         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_141720, whole genome shotgun sequence.
ACCESSION   DASTYP010000256 DASTYP010000000
VERSION     DASTYP010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141720"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFV59_11095"
     CDS             complement(<1..248)
                     /locus_tag="VFV59_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009748023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5326417.1"
                     /translation="MTAPALEVDVVVVGGGATGAGVLRDLAMRGLTALLVERGDFASG
                     TSGRYHGLLHSGARYIQSDPVSARHCAEENAVLRRIAPA"
     gene            complement(245..850)
                     /gene="pgsA"
                     /locus_tag="VFV59_11100"
     CDS             complement(245..850)
                     /gene="pgsA"
                     /locus_tag="VFV59_11100"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU5326418.1"
                     /translation="MADAAPRPARIGSTANLLGIARILATPVVIVLLLIGTPGTGIVA
                     FVVFCLAAITDFLDGWIARRRGEVSALGVFMDLTADKVLVAGVLVVLVEVGLLPTWIV
                     ATILIRELVIAGVRQMAAAENVVIAARALGKSKTMTTLIGMAVLLLAFDAMTGGLVPS
                     GAADALELTGFWIMVVATVLTLVSGWDYLRGALPMLLGHRP"
     gene            complement(843..1475)
                     /gene="plsY"
                     /locus_tag="VFV59_11105"
     CDS             complement(843..1475)
                     /gene="plsY"
                     /locus_tag="VFV59_11105"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU5326419.1"
                     /translation="MNLLLAAALGYLLGAIPFGLLIGRATRGIDIRQYGSHRTGATNA
                     LRTLGTRAAALVFVLDVAKGVAAVLLANALFGGGALGEWAGAVAGFAAIVGHNWSVFI
                     GFTGGRGVATSSGGLGAMSIWTILILAPIVVGIIWRTRFVSLGSISGGLLAPVITAVL
                     WAFGAATVPAIVYALASGLLVTAAHADNIGRLRAGTERKLGQKEAVEPRG"
     gene            complement(1472..1912)
                     /gene="dhaM"
                     /locus_tag="VFV59_11110"
     CDS             complement(1472..1912)
                     /gene="dhaM"
                     /locus_tag="VFV59_11110"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEU5326420.1"
                     /translation="MTDETPATVGIVVVSHSSELAEATVQLVARLANLQPDQPPLLAA
                     GGMEDGSIGTDAVRIADALSAADRGAGVVVLADLGSAVLSTTTAIDDILEPQLASRVR
                     VSSGPLVEGTFIGAVQASLGDSLEDVLAAANDAGSMDKLGDAGS"
     gene            complement(1990..>3448)
                     /gene="glpK"
                     /locus_tag="VFV59_11115"
     CDS             complement(1990..>3448)
                     /gene="glpK"
                     /locus_tag="VFV59_11115"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU5326421.1"
                     /translation="GTTSSRALLFNKDGTIHDVANQEFPQIYPSPGHVEHDPEAIWDS
                     QVAVAKQVIQSAGASPEDIAAIGITNQRETTVVWDKNTGKPVQNAIVWQSRITAGYCD
                     ELKAAGHEQLFRDKTGLPIDAYFSGTKIRHILKNNPGAQEKAERGELLFGTVDSFLIW
                     RLTEGRVHVVDYSNASRTLLFNIHTLDWDDELLGIMGVPRAMMPTPKPSSEVYGETTL
                     FGGSIKIAGDAGDQQAATFGQACFEPGMAKQTYGTGAFMLINTGEEAVPSKNGLLTTI
                     AWGYGGKVIYALEGSVFIAGAAVQWIRDGLRVITNSADVEKLAGKVADTEGVYMVPAF
                     VGLGAPYWDQYARGAIVGITRGTTIEHIAKATVDSMAYQTREVLEAMEADSGVQLSEL
                     KVDGGAVVNNDLMQFQADILGVPVERPKVSETTALGAAYLAGLAVGFWADTNDVTNNW
                     ALDRRFEPQMDEEKREKLFRGWKKAVQRALDWEEH"
BASE COUNT          518 a         1222 c         1212 g          496 t
ORIGIN      
        1 gcgggtgcga tccggcgcag caccgcgttc tcctcggcgc agtgccgcgc cgacacgggg
       61 tcgctctgga tgtagcgggc acccgagtgc agcaggccgt ggtatcggcc gctggtgccc
      121 gaggcgaagt cgccgcgctc caccagcagc gcggtcaacc cgcgcatcgc caggtcgcgc
      181 agcacgccag cgccggtcgc gccgccgccg acgacgacca cgtcgacctc gagggcgggg
      241 gcggtcacgg ccggtgtccg aggagcatcg gcaatgcgcc acgcaggtag tcccagccgg
      301 acaccagcgt gaggacggtc gccacgacca tgatccagaa gccggtgagc tcgagcgcat
      361 cggcggcgcc cgacggcacg aggccgccgg tcatggcgtc gaaggccagc agcagcacgg
      421 ccatgccgat cagcgtcgtc atcgtcttgc tcttgcccag ggcgcgcgcc gcgatcacca
      481 cgttctcggc ggcggccatc tgccgcacgc cggcgatgac cagctcgcgg atcaggatgg
      541 tcgcgacgat ccaggtgggc aacagcccga cctcgaccag gaccaccagc acgccggcga
      601 cgaggacctt gtccgcggtc aggtccatga agacgcccag cgccgacacc tcgccgcggc
      661 ggcgcgcaat ccagccgtcg aggaagtccg tgatcgccgc caggcagaac accacgaacg
      721 cgacgatccc ggtccccggc gtcccgatca gcagcaggac gatgaccacc ggcgtggcca
      781 ggatccgcgc gatcccgagc aggttcgcgg tcgagccgat gcgcgcgggg cgcggtgctg
      841 cgtcagccac gcggctccac ggcctccttc tggcccagct tgcgctccgt gccggcacgc
      901 aggcgcccga tgttgtcggc atgcgccgcg gtcacgagca gccccgatgc cagcgcgtag
      961 acgatggccg ggaccgtggc cgcgccgaac gcccacagca cggcggtgat caccggcgcc
     1021 agcaggccgc cactgatcga gccgagcgag acgaagcgcg tgcgccagat gatgcccacg
     1081 acgatcggcg ccaggatgag gatggtccag atcgacatgg cgcccaggcc gcccgacgag
     1141 gtcgccacgc cgcggccgcc ggtgaagccg atgaagacgg accagttgtg gccgacgatg
     1201 gcggcgaagc cggcgacggc gcctgcccac tcaccaagcg caccaccgcc gaacagtgcg
     1261 ttggccagca gcacggcggc cacgcccttg gccacgtcca ggacgaagac gagcgccgcc
     1321 gcccgcgtcc cgagcgtgcg cagcgcattg gtggcgccgg tccggtgcga cccgtactgg
     1381 cggatgtcga tgccgcgcgt cgcgcggccg atcagcaatc cgaaggggat ggcgcccagc
     1441 aggtagccga gggcggcggc cagcagcagg ttcacgagcc ggcgtccccc agcttgtcca
     1501 tcgagccggc gtcgttcgcg gcggccagca cgtcctcgag cgagtcgccc agcgaggcct
     1561 ggaccgcccc gatgaacgtc ccctcgacca gcggcccgct cgagacgcgc acgcgcgacg
     1621 cgagctgcgg ctccaggatg tcgtcgatgg ccgtcgtggt ggagaggacc gcgctgccga
     1681 ggtcggccag caccacgacg cccgcgccgc gatcggcggc ggaaagcgca tcggcgatgc
     1741 ggacggcgtc ggtcccgatg ctgccgtcct ccatgccgcc agccgccagg agcggcggct
     1801 ggtcgggctg gaggttggcg agccgggcga ccagctgaac ggtcgcctcg gccagctccg
     1861 atgagtgcga cacgaccacg ataccgaccg tggcgggcgt ctcatcggtc atgcgctggg
     1921 tctccgagag gacggatcga ggcccggagc cggacggctc cgggcgatcg gtccgtggtc
     1981 aggcacagac tagtgctctt cccagtccag cgcgcgctgc acggccttct tccagccgcg
     2041 gaacagcttc tcgcgcttct cctcgtccat ctggggctcg aaccgtcggt cgagggccca
     2101 gttgttggtg acgtcgttgg tgtccgccca gaagccgacc gccaggccgg ccaggtaggc
     2161 ggcgcccagc gccgtcgtct ccgacacctt cggtcgctcc accggaacgc ccaggatgtc
     2221 ggcctggaac tgcatcaggt cgttgttgac gacggcgccg ccgtccacct tcagctcgga
     2281 cagctggacg ccggagtcag cctccatcgc ctccagcacc tcgcgggtct ggtacgccat
     2341 cgagtccacc gtcgccttgg cgatgtgctc gatggtcgtg ccgcgggtga tgccgacgat
     2401 ggcaccgcgc gcgtactgat cccagtacgg agcgccgagg cccacgaagg cgggcaccat
     2461 gtacacgccc tcggtgtcgg cgaccttgcc ggccagcttc tcgacatcgg ccgagttggt
     2521 gatcacgcgc aggccgtcgc ggatccactg caccgccgcg ccggcgatga agacggagcc
     2581 ctcgagggcg tagatcacct tgccgccata gccccacgcg atcgtggtga gcagcccgtt
     2641 cttcgacgga accgcctcct cgccggtgtt gatgagcatg aaggcgccgg tgccgtaggt
     2701 ctgcttggcc atgccgggct cgaagcaggc ctggccgaag gtggccgcct gctggtcgcc
     2761 ggcgtcgccg gcgatcttga tcgagccgcc gaagagcgtc gtctcgccgt acacctcgct
     2821 cgagggcttc ggcgtcggca tcatggcgcg cggaacgccc atgatcccga gcagctcgtc
     2881 gtcccagtcg agcgtgtgga tgttgaacag cagcgtccgg ctggcgttcg agtagtccac
     2941 gacgtggacg cggccctcgg tgaggcgcca gatcaggaag ctgtcgaccg tgccgaacag
     3001 gagctcgccg cgctcggcct tctcctgcgc gcccgggttg ttcttcagga tgtggcggat
     3061 cttggtgccg ctgaagtagg cgtcgatcgg caggcccgtc ttgtcgcgga acagctgctc
     3121 gtgcccggcg gccttcagct cgtcgcagta gcccgccgtg atgcggctct gccagacgat
     3181 ggcgttctgc accggcttgc cggtgttctt gtcccagacg acggtcgtct cgcgctggtt
     3241 ggtgatgccg atggcggcga tgtcctccgg cgaggcgccc gcgctctgaa tcacctgctt
     3301 ggcgacggcg acctgcgagt cccagatcgc ctcggggtcg tgctcgacgt gtccgggcga
     3361 cgggtagatc tgcgggaact cctggttcgc gacatcgtgg atcgtgccgt ccttgttgaa
     3421 gaggagggca cggctcgagg tcgtcccc
//
LOCUS       DASTYP010000257         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_435004, whole genome shotgun sequence.
ACCESSION   DASTYP010000257 DASTYP010000000
VERSION     DASTYP010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435004"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFV59_11120"
     CDS             complement(<1..395)
                     /locus_tag="VFV59_11120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5326422.1"
                     /translation="MATLNRLPGGVGASVSAVTSLLDGRPSTVLDIGCGFGDFARRLR
                     RVHHASIVAADASPEVLAIARDRLVGIEGVEVRHADVRALPLEDGALDVAHASLLLHH
                     LDAADAVTALREMRRVARGGVVVNDLRRGP"
     gene            715..2157
                     /gene="gtfA"
                     /locus_tag="VFV59_11125"
     CDS             715..2157
                     /gene="gtfA"
                     /locus_tag="VFV59_11125"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU5326423.1"
                     /translation="MPADRRAIRNAPQLITYPDSLGGTLAGVAELLEGPLRASFGGVH
                     VLPPFPSSGDRGFAPIDVRSIDPAFGGWDDLRRIGHRFDLALDLIVNHVSRRSAPFVD
                     FERHGRRSPYADLFLTLDKVWPAGEPPPQDVARVFKRRPRPYSDYRIADTGELERVWT
                     TFGPDEPSEQVDIDVRSPLARRMIADLLAFFAEQSVRLVRLDAVGYVVKRAGTSCFMV
                     EPEIGDVLRWIAGVAHEHGLELLTEVHGDVPMQRRLAEDSWAYDFALPGLVLHAVLTG
                     SADHLVRHLRTAPRRIVTTLDSHDGIPLQPDLAGALPLDGVRLLASAAVQAGGNLSRV
                     YTADGLPDPAFDAHQANCTWYGAAGGDDDAFLLSRAIQLFAPGIPQVYYVGLLAGDND
                     PAAVARTGEGRAINRHDYTAEEIADALQRPVVRRQLELLELRAAHPAFEGALSVEGGD
                     GRLRLAWENGDARCVLDADLQRRRWLLDAS"
     gene            complement(2154..2924)
                     /locus_tag="VFV59_11130"
     CDS             complement(2154..2924)
                     /locus_tag="VFV59_11130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU5326424.1"
                     /translation="MSATPFTKAEMMIVAAARQLAGKRVCFVGVGLPNIAVNLARRTV
                     APDLELVYEAGVFGARPARLPLSIGDPTIVTGATASVSMFDLFAFYLQGGLVDVGFLG
                     GAQIDRFGNLNTTVIGDYERPAVRLPGSGGACEIVINAREIFVIMRQGRRSFVETLDF
                     QTSPGSPKARPADALAGTRRPGAGPTVVVTDLAVYGFTDGEMTVATLHPGVTRDDVRA
                     AMAWEPRWAADAGETEPPSDEELRLIRQELDPDGVYAG"
     gene            complement(2921..3823)
                     /locus_tag="VFV59_11135"
     CDS             complement(2921..3823)
                     /locus_tag="VFV59_11135"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU5326425.1"
                     /translation="MRDAIAELVRDGDTVAIEGFTHLIGFAAGHEIIRQGRRDLTLAR
                     LTPDLVYDQMVAAGVASKLVFSWLGNPGVGSLHAIRRRVEADAPADGRLALEEYSHFG
                     MVARYTAGASNLPFFPLRSYIDSDLPRANPMIRPIASPYGDETLYAVPPLKPDVTIVH
                     AQRASAAGDTQVWGLVGCQKEAAFAAERVIVVVEELVDDAVIRADPNRTLIPGLIVGA
                     VVVEPWGAHPSYAQGHYDRDNAFYLAWDEISRDEDRLQGWLSEWVHGVAGRGEYVEKL
                     GTERLASLRPGPAPSGEVDYGSYR"
BASE COUNT          542 a         1457 c         1366 g          541 t
ORIGIN      
        1 ggtccgcggc gcaggtcgtt caccaccaca ccacccctgg ccacgcgacg catctcacgg
       61 agggcggtca ccgcgtcggc ggcgtccagg tggtggagca gcagggaggc gtgcgcgacg
      121 tccagcgcgc cgtcctcgag cggcagcgcc cgcacgtcgg cgtggcggac ctcgacgccc
      181 tcgatgccga cgagccgatc gcgcgcgatg gccagcacct cgggactcgc atcggccgca
      241 acgatcgagg cgtggtgcac gcgccgcagc cggcgggcga aatcgccgaa gccacagccg
      301 atatccagca ccgtggacgg gcggccgtcg aggagggacg tgacggcact cacgctcgcg
      361 ccgacgccac cgggcagccg gttgagggtc gccatctccc gaaggttccg gaacagctcg
      421 cgcgcgtcga gcccgggtgc gtcgagcagc tcggtctcgg tggcgcggcg tcggagcagg
      481 aggtcggcga tgcccctgga tcagcactcg tcgtgccacg tggacggcgg acgggccaac
      541 ctcatcgccc gtctggcacg ccggctgctc aagcgacgcg cacagccgga cctccctcgt
      601 cggcgcgggc gcctcctcct ccgcgccgat gggggcgcag gtccggggcg gcagcccatc
      661 cacccggcac gccacccacc ctgtggtctc acgaccgcgc catactcacc gaccatgcct
      721 gccgaccgcc gcgccatccg gaacgcgccg cagctcatca cctacccaga ctcgctgggc
      781 ggcacgctcg ccggggtggc cgagctgctc gaaggaccgc tccgcgcgtc gttcggcggc
      841 gtgcacgtgc tgccgccgtt cccctcctcg ggcgaccgcg gcttcgcgcc catcgacgtc
      901 cggtccatcg accccgcctt cggtggctgg gatgacctgc ggcgcatcgg tcatcggttc
      961 gacctggcgc tcgacctgat cgtcaaccac gtctcgcggc gctccgcgcc gttcgtggac
     1021 ttcgagcgac acgggcgccg ttcgccgtac gcggacctgt tcctcaccct cgacaaggtg
     1081 tggcccgctg gcgagccgcc cccgcaggac gtggcccgcg tcttcaagcg gcggccgcgg
     1141 ccgtactcgg actaccgcat cgccgacacg ggagagctcg agcgggtgtg gacgaccttc
     1201 gggcctgacg agccctcgga gcaggtggac atcgacgtcc gctcaccgct cgcccgccgc
     1261 atgatcgccg atctgctcgc cttcttcgcc gagcagtcgg tgcgcctggt acgcctcgac
     1321 gcggtgggct acgtcgtgaa gcgggccggc accagctgct tcatggtgga gccggagatc
     1381 ggcgacgtcc tgcgctggat cgccggcgtg gcgcacgagc acggcctcga gctgctgacc
     1441 gaggtccacg gcgacgtccc gatgcagcgg cgcctggccg aggacagctg ggcgtacgac
     1501 ttcgcgctgc ccggcctggt gctgcacgcc gtcctgacgg gcagcgccga tcacctggtg
     1561 cggcaccttc ggacggcgcc gcgccgcatc gtgacgaccc tcgacagcca cgatggcatc
     1621 ccactgcagc ccgacctggc cggcgccctc ccgctggacg gagtgcgcct cctggcgtcg
     1681 gcggcggtgc aggcgggcgg gaacctgagc cgcgtctaca ccgccgatgg cctgcccgac
     1741 ccggcgttcg acgcacacca ggcgaactgc acctggtacg gggccgctgg aggggacgac
     1801 gacgcgttcc tgctcagccg tgcgatccag ttgttcgcgc cgggcatccc gcaggtctac
     1861 tacgtcgggc tgctggccgg cgacaacgac cccgccgccg tcgcgcgcac cggcgagggc
     1921 cgggcgatca accgccacga ctacaccgcc gaggagatcg ccgatgccct ccagcggccg
     1981 gtcgtgcgcc gtcagctgga gctcctcgag ctgcgcgcgg cgcatcccgc cttcgagggt
     2041 gccctgagcg tcgagggcgg cgatgggcgc cttcggctgg cgtgggagaa cggcgacgcg
     2101 cggtgcgtgc tggacgccga cctgcagcga cgtcgctggc tgctcgacgc cagctagccg
     2161 gcgtagaccc cgtccggatc gagctcctgg cgaatcaggc gaagctcctc atcggacggc
     2221 ggctcggtct cgccggcatc ggctgcccag cgtggctccc aggccatggc ggcccgcacg
     2281 tcgtcgcgcg tcacgcccgg atgcagcgtg gcgaccgtca tctcgccatc ggtgaagccg
     2341 tagaccgcta ggtcggtgac cacgacggtc ggccccgcgc ccggtcgtcg cgtgccggcc
     2401 agcgcatcgg ccggacgcgc cttcggactc cccggcgagg tctggaagtc cagcgtctcg
     2461 acgaagctgc gccggccctg gcgcatgatc acgaagatct cgcgggcgtt gataacgatc
     2521 tcgcacgcgc cacccgatcc cggcaggcgg accgctggtc gctcgtagtc gccgatgacg
     2581 gtcgtgttca ggttcccgaa ccgatcgatc tgcgccccgc ccaggaaccc cacgtccacg
     2641 agcccgccct gcaggtagaa cgcgaagagg tcgaacatgc tgaccgatgc ggtcgcgccg
     2701 gtgacgatcg tcggatcgcc gatcgacagc ggcagccggg cgggccgagc gccgaacacg
     2761 ccggcctcgt agaccagctc gaggtccggc gcgaccgtcc ggcgcgccag gttgaccgcg
     2821 atgttcggca gcccgacgcc gacgaagcag acgcgcttgc cggcgagctg ccgcgccgcg
     2881 gccacgatca tcatctcggc cttggtgaag ggcgtggcgc tcatcggtac gacccgtagt
     2941 cgacctcgcc cgacggcgct ggccccggtc gcagcgacgc caggcgttcg gtgcccagct
     3001 tctcgacgta ctcgccgcgc cccgccacgc cgtggaccca ctcgctcagc cagccctgca
     3061 gccggtcctc gtcgcggctg atctcgtccc acgccaggta gaacgcgttg tcgcgatcgt
     3121 agtggccctg cgcgtacgat gggtgcgcgc cccacggctc gacgaccacg gcgccaacga
     3181 tcagccccgg gatcagggtg cggttgggat cggcgcggat gacggcgtcg tcgaccagct
     3241 cctcgaccac cacgatcacc cgctcggcgg caaaggccgc ctccttctgg cagccgacca
     3301 gcccccagac ctgcgtgtcg cccgcggctg atgcgcgctg cgcgtggacg atggtcacgt
     3361 ccggcttgag cggcggtacg gcgtaaaggg tctcgtcgcc gtacggggag gcgatcggcc
     3421 ggatcatcgg gttggcgcgc ggcaggtcgg agtcgatgta cgagcgcagc gggaagaagg
     3481 gaaggttgga cgcgccggcc gtgtagcgcg cgaccatgcc gaagtgcgag tactcctcca
     3541 gcgccagccg cccgtcggct ggcgcatcgg cctcgacgcg acgccggatg gcgtgcaggc
     3601 tcccgactcc ggggttgccc agccagctga agaccagctt cgaggcgacg ccggcggcca
     3661 ccatctggtc gtagaccagg tccggcgtca ggcgggcgag cgtcagatcg cggcggccct
     3721 gccggatgat ctcgtggccg gccgcgaagc cgatgaggtg ggtgaagccc tcgatcgcca
     3781 ccgtgtcgcc gtcgcgcacc agctcggcga tcgcgtcgcg catggaggcg actttcgacg
     3841 tctgcgtcac gaccgcagtg tgatgcctcc gcccgacgct cgccccggca tcccgaacgc
     3901 tccgct
//
LOCUS       DASTYP010000258         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_726907, whole genome shotgun sequence.
ACCESSION   DASTYP010000258 DASTYP010000000
VERSION     DASTYP010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726907"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFV59_11140"
     CDS             <1..765
                     /locus_tag="VFV59_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU5326426.1"
                     /translation="RLRQRLAEAAERGADLAVLPELPLNPWSPAFEDANDDDAEATGG
                     PRAKALSEAAAEAQIGVVGGVIHRAEDGTRTNRSLIFDRQGNLVATYDKLHVPEEPGF
                     WETRHYEPGVAAPDVIEAFGLPIGVQICSDTNRPEGTYILGAKGAMAVVIPRSTEERT
                     YQRWKTVFRANALTACLYVLSVNRPEPEHDVPIGGPSLAFDPDGQLLVETTDRLALVT
                     LEASEVLAARRGYPGHLPIRARLYADAWDDVARRDG"
     gene            complement(767..1453)
                     /locus_tag="VFV59_11145"
     CDS             complement(767..1453)
                     /locus_tag="VFV59_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326427.1"
                     /translation="MTDETVECFNCGASNPEWAQVCRSCGVALRHGEARIVPAERIPT
                     DRDSLISIAAVIGTILVALLVGLFIAGLNPTEPTVGRATPTPSPTLAPTLEPTPTPIP
                     TDTPAPTPAPTPALPGTVTFGTALAPDGTVSEPTDTFTPSANMAYSISMPETFGASQF
                     ENEVVRADGSEVVLPREAISVDPSATVFGYTIGNAASLISAWGPGEYVWQVYVNDAVV
                     ARGTFRFAEG"
     gene            1733..1909
                     /locus_tag="VFV59_11150"
     CDS             1733..1909
                     /locus_tag="VFV59_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326428.1"
                     /translation="MNTTMRRLLGRSVQLTVLATPSSSLADAIAEARRRHAAAAQWLA
                     NAATPAVRATAQPA"
     gene            2063..2488
                     /locus_tag="VFV59_11155"
     CDS             2063..2488
                     /locus_tag="VFV59_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326429.1"
                     /translation="MDQLVQHAAELTLDEAADLYRAYAAHILIQGSDAERQALARARA
                     RAARSQRLDEYEHARHAAAAAWRTALPFTSGPWLAVGRAIANAAGALVVESSLDDRSY
                     QVLYGPWRQAIGSLTPVGPGIGNGRPGSAMLIGGSRRRT"
     gene            complement(2902..3288)
                     /locus_tag="VFV59_11160"
     CDS             complement(2902..3288)
                     /locus_tag="VFV59_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326430.1"
                     /translation="MAAALGIDSLAPREELDPTSENLCRYLDPASGDQIVMVSVVAEE
                     TTAEEAKARTSLIVADHSPDVRAIRGPSGAAVEATTIERCRDRCTTSVFVSLPPYYFA
                     VHLTKDYGDADDARRITELVLDPGFQ"
     gene            complement(3533..3619)
                     /locus_tag="VFV59_11165"
     tRNA            complement(3533..3619)
                     /locus_tag="VFV59_11165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3583..3585),aa:Leu,seq:caa)
     gene            3647..4258
                     /locus_tag="VFV59_11170"
     CDS             3647..4258
                     /locus_tag="VFV59_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5326431.1"
                     /translation="MSDASETTKGTRTPPLPDEAAVIARAATGDRTAFAQLMEHYQSA
                     CYGLAFRLLSDPDQAADATQDAFVHAYRAIASYRGGVFRSWLLRITANASYDILRRAQ
                     RRPSSPLPDAEEGAPELPDLAAVNPVVEATRSELYRHLERALAMLPTDQRTAVVLCDV
                     YGMDYNEVASMTQSALGTVKSRIHRGRLRLRELLMEHRELFTG"
     gene            4278..4901
                     /locus_tag="VFV59_11175"
     CDS             4278..4901
                     /locus_tag="VFV59_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326432.1"
                     /translation="MLNSNHPADERLSALAGHDPEAADDAALVEHVTSCDPCTELIHE
                     LGALRASLAELPDLAPPRPLRLLPPVDEAPAHDGWATWVRRLFAPALTAGAALALVGA
                     VGTTGFGIPSPQSAGAQPEAFQSVSEALASEAAGAGGAAAMEDDDQPRASAGAEIMAG
                     GDDSRASAAPADQLPAQRSPWPMVLFTGVALIVAAALLRWIVVPRAG"
     gene            complement(4914..>5097)
                     /locus_tag="VFV59_11180"
     CDS             complement(4914..>5097)
                     /locus_tag="VFV59_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326433.1"
                     /translation="RAAPRTVRAPGERPVPRTRRPPRPDPMPTTFRDSPWAAILAAAL
                     IVGMLAGAIVVGRLAF"
BASE COUNT          752 a         1804 c         1832 g          709 t
ORIGIN      
        1 cggctcaggc agcgcctggc tgaagcggcc gaacggggag ccgatctcgc cgtcctgccg
       61 gagctgccgc tgaacccctg gagcccggcc ttcgaggacg ccaacgacga tgacgccgag
      121 gcgaccggcg gcccacgggc gaaggcattg tccgaggcgg ccgccgaggc gcagatcggc
      181 gtggtcggcg gcgtcatcca tcgggcggag gacggaacgc ggacgaatcg gtccttgatc
      241 ttcgaccgcc agggcaacct cgtggcgacc tacgacaagc tgcacgtccc cgaggagccg
      301 gggttctggg agacgcgtca ctacgagccc ggtgtcgccg cgccggacgt gatcgaggcg
      361 ttcgggctcc cgatcggcgt ccagatctgc tccgacacga accggccgga gggcacgtac
      421 atcctcggcg cgaagggcgc catggccgtg gtgatccctc gctcgaccga ggagcggacg
      481 taccagcgct ggaagacggt gttccgcgcg aacgcgctga cggcctgcct ctacgtgctg
      541 agcgtcaacc ggccggagcc ggagcacgac gtccccatcg gcgggccgag cctcgcgttc
      601 gacccggacg gtcagctgct ggtggagacg acggatcgcc tggcgctcgt caccctggag
      661 gcgtcggagg tgctcgccgc gcgacgcggg tatcccgggc acctgcccat ccgggcgcgg
      721 ctgtacgccg acgcctggga cgacgtggcg cgccgggacg gctagctcag ccctcggcga
      781 agcggaacgt gccgcgggcg acgacggcgt cgttgacgta cacctgccag acgtactcgc
      841 cggggcccca tgcggaaatc aggctggccg cgttgccgat cgtgtagccg aagaccgtgg
      901 cggagggatc gaccgagatc gcctcgcgcg ggagcacgac ctcgctgccg tcggcacgca
      961 cgacctcgtt ctcgaactgc gaggcgccga acgtctcggg catcgagatc gagtacgcca
     1021 tgttggccga cggcgtgaag gtgtcggtcg gttccgacac ggtgccgtcg ggcgcgagcg
     1081 ccgtcccgaa cgtgaccgtg ccgggcagtg ccggggtcgg tgccggcgtg ggcgccggcg
     1141 tatcggtggg aatcggcgtg ggcgtcggct ccagcgtcgg ggcgagggtg ggggacggcg
     1201 tcggggtagc gcggccgacg gtcggctccg tggggttcag cccggcgatg aacagcccga
     1261 ccagcagcgc gacgaggatg gtgccgatga cggcggcgat cgagatcagc gagtcacggt
     1321 cggtcgggat ccgctcggcc gggacgatcc gcgcctcacc gtgcctcagc gcgacgccac
     1381 acgaccgaca gacctgcgcc cactccgggt tcgaggcgcc gcagttgaag cattcgacgg
     1441 tctcgtcggt catcgggcgt tgcagtgctc ctccgtggct gccagtgggg tagcgccgtg
     1501 gcgggcgaaa gcctagcacg cagaaggccg ccggcggtga gaacgccgac ggccaaacgg
     1561 ccgtcctcgc aacaggtcgg ccgcggccag tctacgtcat ccggccgcgg ctcctccgtc
     1621 gctcctccat ggaccgatgc cgacgggcgt cggatgccaa cggttctcct ggcggccacg
     1681 atgaggccat cgctcagcca cctgcagccc tctcggaacc ccaggagaga ccatgaacac
     1741 gacgatgcgg cgactgctcg gacgctccgt gcagctcacc gtcctggcga caccatccag
     1801 cagcctggcc gatgcgatcg ccgaggcgcg ccgccgccac gcggccgccg cccagtggct
     1861 ggccaacgcc gcgacccctg ccgtccgcgc caccgcgcag cccgcctgag cgctcccgcg
     1921 ctgcggcccg ccggcgggct ggggacaagt gcccttcagc actggtgcac gcgggcgccc
     1981 gacgtaggct ctgccacagg cccaacatcc gcgatcccgg aacgttgata gaagtgtggg
     2041 gacgatggtc ggccgcggtc ccgtggacca actggtccag cacgccgcgg agctgacgct
     2101 ggacgaggca gcggatctgt accgcgccta cgccgcgcac atcctgatcc agggatcgga
     2161 cgccgagcgc caggcgctcg ctcgcgcccg ggcacgtgcc gcgcggtcgc agcgcctcga
     2221 cgagtacgag cacgcccgtc acgccgcggc cgccgcctgg cggacggcgc tgccattcac
     2281 ctccggaccg tggctcgccg tcggacgggc catcgcgaac gcggccgggg ccttggtcgt
     2341 cgagtcgagc ctggacgatc ggtcgtacca ggtgctgtac ggaccatggc gtcaggcgat
     2401 cggctcgctg acgccggtcg gcccgggtat cggtaatggc cgtcccggct cagcgatgct
     2461 catcggtgga tcccggcgca ggacgtaagc cgaggcgttc gtacgtcgca tggccggtgt
     2521 gcgacgcggt gcggccgcgc accgtggtcg gatcatgtgc acccgagact ttcagcgaac
     2581 cgcgagtgtc gcgcgctccg cggacgcggg gtggtcccac gccggctacg tcgtccggct
     2641 tggcgccgac gggaagttcc gacgcgagtt cgtggactgc gacgcggccc atggacgccc
     2701 ggcgacagcc gtccggatgc tcccgttaca gaagacggcg ccgcgccgcg cgggttgggg
     2761 ctggagcgga ctgcgaagct ctccggccgg cgcttgaggg ccgcggcctg agtcgagcgc
     2821 ccaggcgcgg gatcgggtga tttgggctgg gatgccgtac ggcacttcgc gagaagcgct
     2881 gaccgcacgg cgtcgctccg gtcactggaa gcctgggtcg agcacgagct cggtgatacg
     2941 ccgagcgtcg tcggcgtcgc catagtcctt ggtcaggtgc accgcgaagt aatacggcgg
     3001 gaggctcacg aacacggagg tcgtgcacct gtcacggcac cgttcgatcg tcgtcgcctc
     3061 caccgcggcg cccgacggac cgcgaatggc ccgaacatcg ggcgagtgat cggcgacgat
     3121 gagggacgtt cgggccttcg cttcttcggc ggtcgtttcc tcagccacga ccgacaccat
     3181 gacgatctgg tcgcccgacg ccgggtccag gtagcggcag aggttctcgc tggtcgggtc
     3241 gagctcttcg cgtggtgcga ggctgtcaat tccgagtgcc gccgccatgg ccgaaggctc
     3301 gacaaaggcg cagggatgat cgcgaggcgg tccggccggt gtgatcgcac aggctgcagc
     3361 gatgagcgaa agcccgatcg tcgcccacag gatacggcgc ccactcgcca ctcggtcatg
     3421 tctcccgtct gggtgtattc gactacgccg cgctccatgt cgaagtgcta ccagaacgcg
     3481 cacgccaacc cggcgatggg cgccagagtc ggccgagccg tgctcaagga cgtggtaccg
     3541 gaggtggggg tcgaacccac acgggctttc gcccatcggt gtttgagacc gacgcgtctg
     3601 ccgattccgc cactccggca acgggaggct tcattatagg aagccgatgt ccgacgcctc
     3661 cgagacgacc aagggcacca gaacgccgcc gcttcccgac gaagcggcgg tcatcgcgcg
     3721 tgccgccacg ggagaccgca cggcgttcgc gcagctcatg gagcactacc agtccgcctg
     3781 ctacggactg gccttccggc tcctgagcga ccctgaccag gccgccgatg ccacccagga
     3841 cgcgttcgtc catgcctacc gcgccatcgc ctcgtaccgc ggtggcgtct tccggtcctg
     3901 gctgctgagg atcacggcca acgcgagcta cgacatcctc cgtcgagccc aacgccgacc
     3961 gtcatcccca ttgcccgatg ccgaggaggg ggcacccgag cttcccgatc tggcggccgt
     4021 caacccggtg gtcgaggcga cgcgcagcga gctctaccgc cacctggagc gcgcgctggc
     4081 gatgctgccc acggaccagc gcacggccgt cgtgctctgc gacgtctacg gaatggacta
     4141 caacgaggtg gcatccatga cgcagtcggc gctggggacc gtcaagtcac gcatccaccg
     4201 cggccgactg cggttgcgag agctcctcat ggagcaccgg gaactgttca ccggatgagc
     4261 gcgtctggaa ccacggcatg ctgaactcga accaccccgc cgacgagcgg ctctcggccc
     4321 ttgccgggca cgacccggag gccgcggacg acgccgccct cgtcgagcac gtcacctcct
     4381 gcgacccatg caccgagctc atccacgagc tcggtgcgct gcgtgcgtcg ctggccgagc
     4441 tgccggatct cgcgccgcca cggccgctcc gactcctccc acccgtcgac gaggccccgg
     4501 cccacgacgg gtgggcgacc tgggtccggc gcctcttcgc gccggcgctg acggcggggg
     4561 cggcgctcgc gctcgtcggt gcggtcggga cgaccgggtt cggtattccg agtccacaga
     4621 gcgccggcgc ccagccggag gccttccagt cggtctcgga ggcactggcc agcgaagcag
     4681 ccggggccgg cggggcggcc gccatggagg acgacgacca gccgcgtgcg tccgctggtg
     4741 ccgagatcat ggccggcggc gatgacagcc gcgcgtcggc ggctcccgcc gaccagcttc
     4801 cggcgcagcg ctctccgtgg ccgatggtgc tgttcacggg cgtcgcgctg atcgtcgcgg
     4861 ccgcgctcct gcgctggatc gtggtgccgc gcgccggata gcggcgacgc tcgctagaac
     4921 gccagccggc ccaccacgat cgcgccggcc agcatcccca cgatgagtgc ggccgcgagg
     4981 atggccgccc atggcgagtc gcggaaggtc gtgggcatcg gatcggggcg cggtggacgc
     5041 cgcgtgcgcg ggaccggccg ctcccccggt gcgcgcaccg tccgcggggc cgcccgc
//
LOCUS       DASTYP010000259         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U2445
            k141_1347884, whole genome shotgun sequence.
ACCESSION   DASTYP010000259 DASTYP010000000
VERSION     DASTYP010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960903
            Sequence Read Archive: SRR25158459
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:17:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,239
            CDSs (total)                      :: 2,201
            Genes (coding)                    :: 2,189
            CDSs (with protein)               :: 2,189
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 12 of 12
            Pseudo Genes (internal stop)      :: 0 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347884"
                     /isolate="SMAG_U2445"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.64527778 N 117.4222222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFV59_11185"
     CDS             <1..597
                     /locus_tag="VFV59_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5326434.1"
                     /translation="LGRTGRPIPARHLAATEGLFVGTAPPYEMVRIGLGWYGELGIGV
                     TPTPELAGIAAELRPALRVVARPVRLETVAAGSSVGYGGEWTAGRASRIATLPIGYAD
                     GWSRASWPGGVALAGGRRVPIAGRVSMDSVCVDVTDVEGMGMDDEVVLLGAQGDERIT
                     ANEVAALRRTIPNEVLSSLGARLPRVFVGSDQFAGAAR"
     gene            723..2333
                     /locus_tag="VFV59_11190"
     CDS             723..2333
                     /locus_tag="VFV59_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5326435.1"
                     /translation="MAVEPADVLIVGAGASGGVVAKRLAEAGFDVVCLEQGDWRDKGE
                     YPGVRLDWELSARKEWATSPNIRGLPEDYPIVEDSPVSPLMFNGVGGSMLIFAGAWPR
                     MLPSDFRVRSLDGVADDWPLTYDELLPYFRQTDRDFGVSGLAGDPAYPPDPEDPPMPP
                     LPIGAAGMKVARAHTRLGWHWWPEFNSINSVPYDGRRPCVQRSTCQSGCNEGAKASTD
                     LTHWPKAIAKGARLITGARVRKIETNAQGLATGANWIDRDGNEHFQPAKVVVLAANAI
                     GTARLLLLSADSRHPDGLANSSGLVGKRLMMHPFANVAGLFDEPLTSWQGQFGDLIES
                     LEFYETDERRGFVRGARWGLAPTGGPINTALPSRAGTQVWGPDHHLHVRSHLGHGANW
                     GLFAEDLPDEANQVSLSQEVTDSSGIPAPEIHYSMGDNARRMLDFHIERATESLLEAG
                     AHTIEVDRLMRYSGWHLLGTARMGDDPTTSVLDRWNRAHDVPNLYVVDGSCFVTSSGV
                     NPTSTIVALALRAAEHMVETRFEQQVPA"
     gene            2330..>2710
                     /locus_tag="VFV59_11195"
     CDS             2330..>2710
                     /locus_tag="VFV59_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5326436.1"
                     /translation="MSEHETGAPTDVTAGMPVTDETVRHGDGDAAAEDALAAFDDGRR
                     RTFAAIADTLIPEAHGMPSAASVVTEQRLRFVFRSRPDLAEPFLAALRPDLGEDLRGR
                     LDALERDEPTNLAALQLVLVGAYYT"
BASE COUNT          392 a          985 c          957 g          376 t
ORIGIN      
        1 ctgggccgga ccggccgccc gattcccgcg cgtcacctgg ccgcgaccga gggcctcttc
       61 gtcggcaccg ccccgccgta cgagatggtc cgcatcggcc tcggctggta cggcgagctg
      121 gggatcggcg tcacgcccac cccggagctc gcgggaatcg ctgccgagct gcggccggcg
      181 ctgcgggtcg tggcgcggcc ggtgcgcctg gagacggtcg ccgccgggag ctcggtcggt
      241 tacggcggcg aatggacggc gggtcgagcc tcgcgcatcg cgacgctgcc catcggctac
      301 gccgacggct ggtcgcgcgc gtcgtggccg ggcggcgttg cgctcgccgg cggccgacgg
      361 gtaccgatcg ccggccgcgt ctcgatggac tccgtctgcg tcgacgtcac cgacgtggag
      421 ggcatgggga tggacgacga ggtcgtgctg ctgggcgcgc agggtgacga acggatcacc
      481 gccaacgagg tcgccgccct ccggcgcacc atcccgaacg aggtgctctc ctccctcggg
      541 gcacggctgc cgcgcgtctt cgtcggctcc gaccaattcg ccggcgcggc ccgctgacgc
      601 atcggttcgc gccccttctg ctcggcatag agaacgttta ctgtaaggtc gagcgaagcg
      661 gccgggacga cgtcgacgag gcgatgaccg ccggcccgcg gcgaatccga aaggagtgat
      721 cgttggccgt cgaaccagca gatgtcctca tcgtcggcgc cggggcgtcc ggcggcgtcg
      781 tggcgaagcg cttggccgaa gcgggcttcg acgtcgtctg cctggagcag ggcgactggc
      841 gggacaaggg cgagtacccc ggggtgcggc tcgactggga gctctccgcg cgcaaggaat
      901 gggcgaccag cccgaacatc cgcggcctgc cggaggacta ccccatcgtc gaggactctc
      961 ccgtctcccc gctgatgttc aacggtgtcg gcggctcgat gctgatcttc gccggcgcgt
     1021 ggccacggat gctgccgtca gacttccggg tccgatcgct cgacggcgtg gccgacgact
     1081 ggccgctgac ctacgacgag ctcctcccct acttccgcca gaccgatcgc gacttcgggg
     1141 tctccggcct cgccggcgat ccggcctatc caccggatcc ggaggatcca ccgatgcccc
     1201 cgctacccat cggcgcggcc ggcatgaagg tggcgcgagc gcacacgcgg ctgggatggc
     1261 actggtggcc cgagttcaac tccatcaact cggtccccta cgacgggcgc cggccatgcg
     1321 tccagcgcag cacctgccag tcgggctgca acgagggggc aaaggcgtcc accgacctca
     1381 cgcactggcc gaaggccatc gcgaagggtg cccgcctcat caccggcgcg cgggtccgga
     1441 agatcgagac caacgcgcag ggactggcga ccggagcgaa ctggatcgac cgcgacggca
     1501 acgagcactt ccagccggcg aaggtcgtgg tgctggccgc gaacgccatc ggcaccgccc
     1561 gactgcttct cctgtccgcc gactcccggc atcccgacgg gctcgccaat tcgtccggcc
     1621 tggtcgggaa gcggctgatg atgcacccgt tcgccaacgt ggcagggctc ttcgacgagc
     1681 ccctgaccag ctggcagggc cagttcggcg acctgatcga gtcgctggag ttctacgaga
     1741 ccgatgagcg ccgcggcttc gtgcgtggcg cgcgctgggg actggcgccg accggcggac
     1801 cgatcaacac cgcgctcccc agccgagccg ggacgcaggt ctggggtccc gaccaccacc
     1861 tgcacgtccg ctcgcacctc ggccacggtg ccaactgggg cctcttcgcg gaggacctgc
     1921 cggacgaggc caaccaggtg agcctgtcgc aggaggtcac cgactcctcg ggcatccccg
     1981 cgccggagat ccactactcg atgggcgaca acgcgcgacg catgctcgac ttccacatcg
     2041 agcgcgccac cgagtcgctg ctcgaggcgg gggcgcacac catcgaggtc gaccggctga
     2101 tgcgctactc gggctggcac ctcctgggca cggcgcgcat gggcgacgat ccgacgacat
     2161 cggtcctcga tcgctggaac cgcgcccacg acgtgccgaa cctgtacgtc gtcgacggca
     2221 gctgcttcgt gacgtcgagc ggcgtcaacc cgacctccac catcgtcgcg ctggcgctgc
     2281 gggcggcgga gcacatggtc gagacccggt tcgagcagca ggtgccggca tgagcgagca
     2341 cgagaccggg gccccgaccg acgtcaccgc ggggatgccc gtgaccgacg agaccgtccg
     2401 gcacggcgac ggagacgcgg cggccgagga cgcgctggcg gccttcgacg acggccgtcg
     2461 gcggaccttc gccgccatcg cggacacgct gatccccgag gcgcatggca tgccctccgc
     2521 ggcatcggtc gtgaccgagc agcggctgcg cttcgtgttc cgttcgcggc ccgacctggc
     2581 ggagccgttc ctggccgccc tccggcccga cctgggagag gacctccggg gccgcctgga
     2641 cgccctcgag cgggacgagc ccacgaacct ggccgcgctg cagctggtgc tcgtcggcgc
     2701 ctactacacg
//
